LOCUS       DAGCYL010000001        53789 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.001, whole genome shotgun sequence.
ACCESSION   DAGCYL010000001 DAGCYL010000000
VERSION     DAGCYL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.001"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            64..762
                     /locus_tag="MB216_000001"
     CDS             64..762
                     /locus_tag="MB216_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBT4524868.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(930..3569)
                     /locus_tag="MB216_000002"
     CDS             complement(930..3569)
                     /locus_tag="MB216_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBT4524869.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(4188..5219)
                     /gene="queA"
                     /locus_tag="MB216_000003"
     CDS             complement(4188..5219)
                     /gene="queA"
                     /locus_tag="MB216_000003"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBT4524870.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            5412..6587
                     /locus_tag="MB216_000004"
     CDS             5412..6587
                     /locus_tag="MB216_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBT4524871.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            6591..7232
                     /locus_tag="MB216_000005"
     CDS             6591..7232
                     /locus_tag="MB216_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4524872.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            7229..8146
                     /locus_tag="MB216_000006"
     CDS             7229..8146
                     /locus_tag="MB216_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4524873.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            8143..8814
                     /locus_tag="MB216_000007"
     CDS             8143..8814
                     /locus_tag="MB216_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4524874.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(9355..9843)
                     /locus_tag="MB216_000008"
     CDS             complement(9355..9843)
                     /locus_tag="MB216_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBT4524875.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(10044..10310)
                     /locus_tag="MB216_000009"
     CDS             complement(10044..10310)
                     /locus_tag="MB216_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBT4524876.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(10310..10573)
                     /locus_tag="MB216_000010"
     CDS             complement(10310..10573)
                     /locus_tag="MB216_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBT4524877.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            11049..11234
                     /locus_tag="MB216_000011"
     CDS             11049..11234
                     /locus_tag="MB216_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4524878.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(11376..13199)
                     /locus_tag="MB216_000012"
     CDS             complement(11376..13199)
                     /locus_tag="MB216_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBT4524879.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(13797..16502)
                     /locus_tag="MB216_000013"
     CDS             complement(13797..16502)
                     /locus_tag="MB216_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBT4524880.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(16808..17755)
                     /locus_tag="MB216_000014"
     CDS             complement(16808..17755)
                     /locus_tag="MB216_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBT4524881.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            17945..18214
                     /gene="rpsN"
                     /locus_tag="MB216_000015"
     CDS             17945..18214
                     /gene="rpsN"
                     /locus_tag="MB216_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBT4524882.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(18390..18752)
                     /locus_tag="MB216_000016"
     CDS             complement(18390..18752)
                     /locus_tag="MB216_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBT4524883.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(18763..19884)
                     /locus_tag="MB216_000017"
     CDS             complement(18763..19884)
                     /locus_tag="MB216_000017"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBT4524884.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(19900..20250)
                     /locus_tag="MB216_000018"
     CDS             complement(19900..20250)
                     /locus_tag="MB216_000018"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBT4524885.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(20380..21891)
                     /locus_tag="MB216_000019"
     CDS             complement(20380..21891)
                     /locus_tag="MB216_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBT4524886.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(22213..23565)
                     /locus_tag="MB216_000020"
     CDS             complement(22213..23565)
                     /locus_tag="MB216_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBT4524887.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(23718..24794)
                     /locus_tag="MB216_000021"
     CDS             complement(23718..24794)
                     /locus_tag="MB216_000021"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBT4524888.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            24990..26735
                     /locus_tag="MB216_000022"
     CDS             24990..26735
                     /locus_tag="MB216_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBT4524889.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(27015..27452)
                     /locus_tag="MB216_000023"
     CDS             complement(27015..27452)
                     /locus_tag="MB216_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBT4524890.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(27436..28809)
                     /locus_tag="MB216_000024"
     CDS             complement(27436..28809)
                     /locus_tag="MB216_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4524891.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(29131..29619)
                     /locus_tag="MB216_000025"
     CDS             complement(29131..29619)
                     /locus_tag="MB216_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4524892.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(29641..30243)
                     /locus_tag="MB216_000026"
     CDS             complement(29641..30243)
                     /locus_tag="MB216_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBT4524893.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(30747..31376)
                     /gene="udk"
                     /locus_tag="MB216_000027"
     CDS             complement(30747..31376)
                     /gene="udk"
                     /locus_tag="MB216_000027"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBT4524894.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(31460..32155)
                     /locus_tag="MB216_000028"
     CDS             complement(31460..32155)
                     /locus_tag="MB216_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBT4524895.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(32168..32884)
                     /locus_tag="MB216_000029"
     CDS             complement(32168..32884)
                     /locus_tag="MB216_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBT4524896.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            33214..33819
                     /locus_tag="MB216_000030"
     CDS             33214..33819
                     /locus_tag="MB216_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBT4524897.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            34144..34467
                     /locus_tag="MB216_000031"
     CDS             34144..34467
                     /locus_tag="MB216_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBT4524898.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            34649..36019
                     /locus_tag="MB216_000032"
     CDS             34649..36019
                     /locus_tag="MB216_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBT4524899.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(36149..37450)
                     /locus_tag="MB216_000033"
     CDS             complement(36149..37450)
                     /locus_tag="MB216_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBT4524900.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(37680..39638)
                     /locus_tag="MB216_000034"
     CDS             complement(37680..39638)
                     /locus_tag="MB216_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBT4524901.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(39815..40081)
                     /locus_tag="MB216_000035"
     CDS             complement(39815..40081)
                     /locus_tag="MB216_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBT4524902.1"
                     /translation="MLNYLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(40591..42717)
                     /gene="pnp"
                     /locus_tag="MB216_000036"
     CDS             complement(40591..42717)
                     /gene="pnp"
                     /locus_tag="MB216_000036"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBT4524903.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(42905..43174)
                     /gene="rpsO"
                     /locus_tag="MB216_000037"
     CDS             complement(42905..43174)
                     /gene="rpsO"
                     /locus_tag="MB216_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBT4524904.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            43425..43988
                     /locus_tag="MB216_000038"
     CDS             43425..43988
                     /locus_tag="MB216_000038"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBT4524905.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(44241..44762)
                     /locus_tag="MB216_000039"
     CDS             complement(44241..44762)
                     /locus_tag="MB216_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBT4524906.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(44765..45607)
                     /locus_tag="MB216_000040"
     CDS             complement(44765..45607)
                     /locus_tag="MB216_000040"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBT4524907.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(45600..46325)
                     /locus_tag="MB216_000041"
     CDS             complement(45600..46325)
                     /locus_tag="MB216_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBT4524908.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            46501..46797
                     /locus_tag="MB216_000042"
     CDS             46501..46797
                     /locus_tag="MB216_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4524909.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            47365..48957
                     /locus_tag="MB216_000043"
     CDS             47365..48957
                     /locus_tag="MB216_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBT4524910.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            48970..49710
                     /locus_tag="MB216_000044"
     CDS             48970..49710
                     /locus_tag="MB216_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBT4524911.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            49878..51227
                     /locus_tag="MB216_000045"
     CDS             49878..51227
                     /locus_tag="MB216_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4524912.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(51355..51945)
                     /locus_tag="MB216_000046"
     CDS             complement(51355..51945)
                     /locus_tag="MB216_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBT4524913.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            52092..53444
                     /gene="brnQ"
                     /locus_tag="MB216_000047"
     CDS             52092..53444
                     /gene="brnQ"
                     /locus_tag="MB216_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBT4524914.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT        15401 a        11203 c        10115 g        17070 t
ORIGIN      
        1 atgacagaaa tattttttga tacatggtat attggtgtta ttaaagaaaa ggagggaaaa
       61 atcatgcaat cttctgcaga attaaaacgc caagcaaaag agagtctaaa aggacgttgg
      121 ggacaagctg ttttactaaa tctaatccct acattgattg gtattgctac tattcttctt
      181 attatgattc ctattatctt gtttgccacc accatatatg atggatcagc gagcgtctca
      241 gaagcgacaa cttcaggagg cggtagttca ggaggcggtg gcggaattat ttccagcatc
      301 attagcgctt tgttcatgag cggtatttct tggacttatc ttgatatttt acgtggacaa
      361 aaagatcaga tcgaaccatt caaagatgca tttcgtggat tcaaaggttt gtttatcggc
      421 ggagtgatca gcttagctgt gctgatctcg atctttacaa cattatgggg tttgctatta
      481 gttattcctg gaatcattaa aagctattct tattctcaaa gctattttat ttactacgat
      541 atcgtccaag aaacaggaca aacacctaaa gtactggata cgatcactgc aagtcgaaaa
      601 ttgatggatg gttataaagg acaacttttc tggttagatc tttcatttat cggctggcat
      661 atcttagcat tagcaactgc tggtattggt tatctatggc tcagcccata catttctgca
      721 acaaaagcag cattttatga acaattgcca aaagatattt aaaagagagt gtgacttgtg
      781 accgaggggg tggcaggaga acaccgttta tcgagaggca gtaaaagaaa cattcagccc
      841 attagcaaaa aggaggtgtg acaacattca tgtcacacct cctttttgta tagttccagt
      901 aaaaagaaaa tcaacttaga aacagttaat cattcctctt cttctgcttc cggtaatcga
      961 tggaacttga tcagttcatt caccgcgatt ggtacgatag ataaaatccc taccaagaac
     1021 cagtgattca aactgatgtg tactaggccg aatagttctt gcgtaaatgg cagcaaagca
     1081 acagccgttg taatagctaa tgccagtaac gccatttcta ccaaagcttt attggtcgct
     1141 aaattgactt tgaagatcga ttggctgctg cgtacattga acacatgcag aatcgatgaa
     1201 taagctaaga tcaagaatgc aacagtttga ccgacatgct gacttgcgtc tacccatggg
     1261 ctgatagtat cgacataact acctaaataa taaccaaaca aggttacgat ggtgaagacc
     1321 cctgcattta tggcaatttt ttgcaataaa cctcgagcaa agatcccttc atttttcgga
     1381 atgggtggct gatccatgat gccgtgttcc gctggctctc ttcctaaagc aaatcctgga
     1441 agtccatcag caactacatt gacaaacagt agttgcacag ctgtaaatgg tgctccccat
     1501 cctaacagca tcgcaatcaa aacaataaag atctccgaaa tattacagct tagcaaaaag
     1561 ttgatagctt tacgaatatt ctgataaacc gaacgtcctt gactgacagc gtctacgatc
     1621 gtcgcaaagt tgtcatccgt caaaatcata tctgcggcac tttgcgccac atctgttcct
     1681 gtgatcccca tggcacaccc aacgttactt gctttcaacg ctggtgcatc attcacacca
     1741 tccccagtca tcgctacgac tgcacccgta cgttgccaag atttgacgat ccggatcttg
     1801 tcttctggcg ttacccgagc atagacagag agagctttga cacgcgcatc taattcttcg
     1861 tctgataatt gatgcagttc tgatcctgaa agtgcttctg acggatcatt caagatacct
     1921 aattctttcg ctatggcact ggcagtcacc acatgatctc cagtgatcat gactgttttg
     1981 atgccagcct ttttcgctcg cttgattgct cctttacttt ctggacgtgg tggatcgatc
     2041 attccgatca gaccaatcaa acgtaagtct ttttccagtg cttcagaaga aatatcttgc
     2101 ggttcttcgt cataagtagc ataaccaaca gcaatcactc tcagtgcccg tttaccaaag
     2161 cgatcattaa cgatcgccgc ttgttctaca tcgcctgaac taaagcgcgg agcaagaaca
     2221 tcgaatgctc ctttggtgat tgagatgtat ttttcgccca tttggtgaac tgttgtcatc
     2281 atttttcgtt cagaatcaaa aggaagctcg tttactcgtg gatatttttc ttctaattct
     2341 gcctttgtat gataattctc ttcaacagcc cttacgatgg ctacttctgt tggattacct
     2401 ttgataacaa ggtcgtcatc ttcttgctcg accgtcacat ctgtacatag tgcagccatt
     2461 ttcaatattt ccatcgcgct gttcgtcatt gaatcttcga tattcgttac ttcatcttct
     2521 ttgctccata cccggcggac gcgcatttca ttttgtgtca gcgttcctgt tttgtctgag
     2581 cagataacat tagcggttcc tagcgtttca actgctggca actgtcggat gatcgcatgt
     2641 ttcctcgcca tcttctgcac accgtatgca agtgtcaacg taacaataac agtcaaggtt
     2701 tccggtacag ctgcaacagc caaggaaatc gaagtcatga acatctcaag gagtggttct
     2761 ccttgcagtt caccaatcac aaatactaaa gccgcagccg ctaaagcgat catactgatt
     2821 cgtttgccta gctgattcaa tcggacctgt aaaggtgttt tttgcatagt attttcgttc
     2881 aaaagatccg cgatcttacc catttctgta gccatgccaa tggcagtgac gatcgctttc
     2941 ccacgacctg cagcaaccgt acatccttta aataccatat taagttggtc acctaatgac
     3001 tcatcttctt cagcaataaa tgctgcatct ttttcaactg cttcactttc tccagtcaac
     3061 gccgattctt ctacacgtaa ttgtgatgct tcgatcaaac gggcatcggc agaaatagca
     3121 tcaccggact ctaaaacaag aatatcaccc actaccagtt gctctgactc aacagtggtc
     3181 actttgccgt ctcgtatgac agttgccgtt tgtttattca tatcttgcaa ggattccagt
     3241 gctttttctg cgtttccttc ttggacaatc gccaataccg agttgatcac aacgatagcg
     3301 atgatcagca gtccttcaaa taaatcccca tgttctgtag caaatgcggt ataaaaggaa
     3361 atcaccgctg ctacaagaag gatgatcgtt gtaaaatcgg acaagcttcg aaagaatttt
     3421 ttgatcatcg attctttcgg cgcctcatcg aatttattca atccctgctt ttttcgccgt
     3481 tcatccactt cttgtgaaga caacccagta tctggatctg cttctgttac cttctttact
     3541 tcttctattg tctgcttgta ttcttccatc ctattcctcc tttaaaatct tcgtacaaaa
     3601 aaaggtactt attttactat aacatccttt ttttatttac ttcaaataaa gggcttatcg
     3661 attatttgtt aagaaagcgt aaaatttaaa gtgcttatat ttgcaaacgc taccaaagga
     3721 tgctattata tagatgtaag gaaggaggat accatcaaat agacagaaga atttctcgat
     3781 agaacagtgt aagcaaacaa agttgttaga agtacaggtt tcacccagac atgcgtgtag
     3841 agtacaaacc tataaatagt gaatgcgtat ggatgtttgg atcacgaagt atccgaaaca
     3901 agaagtaatg cgatacgtaa caattgaata acgtaaacgt ctgaaaattg aataggaaca
     3961 agagatgttt caaatgttga agatgtaacg aaaacaaaat ttttgtttaa catgaagaaa
     4021 ttcaacaccg tgctttgcat gtatgatgga aagttacaat catttctgtt tatttgaatc
     4081 cttccgaggt acaagaaagc cgagcttatc gagagctcgg ctttttcttt gtcataaaat
     4141 ctttgtaaag cttttttgtt attcaaaact agcggctttt cataacctta tttcacgaac
     4201 atcgcatctc caaaactaaa gaaacgatag cgctcttcaa tcgcatgctg gtaagcagat
     4261 aaagtcaatt ctcttcctgc aaaggcacta actaacatga ctaaagtcga ttttggcaaa
     4321 tggaaattag ttgagaaagc ttctaccact ttgaattcat agccaggcgt aatgaaaata
     4381 tcagtccatc cgctgtctgc tttgatttct ccattaaatt tcgttccaat cgtttctaat
     4441 gttcgaattg aagttgttcc gacagccacg attttcccgc catttttacg tacttggttc
     4501 aatcgctcag ccgcttcttc cgtcaaacgg tagaattcac tatgcatatg gtgttcttca
     4561 attttatcta cgctgacagg tcggaatgta cctaacccta catgtagtgt cagatagact
     4621 aattcaacac cctttgcttc gatctcatca agtaattctt tcgtaaaatg caatccagct
     4681 gttggcgctg cagcagaacc attttctttc gcatagacag tttgatagcg atctgggtct
     4741 tccagacgtt ctttgatata tggaggcaat ggcatttctc ccaatgattc aagaatctct
     4801 agaaaaattc cttcatactc aaagtcaatg atccgtccgc cgtgttccaa ttcttctttc
     4861 actactgctt gcaatctgcc atcaccaaaa gagattcttg tcccaacttt ggcacgtttc
     4921 gcaggtttga tcaatgtctc ccacgtatct ccttgtgtat tcgttagaag aaggacttct
     4981 aaatgcgcac cagtatctgg tttttctcca tataaacgag caggcagaac tcgtgtatta
     5041 ttcataacta gcgcatcacc aggctgtaac tcttcaatga tttcatggaa atgtttatct
     5101 tccatctctc cagttttccg atccaaaaca agtaaacgag aatgatcgcg gtcttttaat
     5161 ggtgtttgtg cgattaattc ttccggcaag tcaaaatcaa aatcttctgt tgttaacata
     5221 tcgtcactca ctttctatca tgtgcttcat cattctatca tgtttttcgt tctgttggca
     5281 aaatcagcac tttttcactt ctattttttg ttttcctaaa taaaaaaact accctatttg
     5341 tattttttca ttgttttagg catactagta atagatagtt cttcattgaa aatagaaaaa
     5401 gaggtggcac catgattgaa tttcaaaatg tgtcaaaaat ttataaaggt ggaaaagtcg
     5461 ctgtagagga cgtcaatctt tcatttgaaa agggtgagtt catttgtttt atcggtacga
     5521 gcggaagcgg aaaaacgacg acgatgcgga tgatcaaccg aatgactgat ccaacacaag
     5581 ggaaaatttt gattgatgga aaatccatcc aagaaattga tccagtggaa ttgcgacgtc
     5641 agatcggcta tgttatccaa agtattggat tgatgcctca catgacgatc cgtgaaaata
     5701 tcactcttgt ccaaaaattg ttgaaagtca gtcaagaaga acggaataaa acagctgaaa
     5761 aaatgattga tcttgtggaa ttgccccgtg agatgttaga tcgctaccca catgaattat
     5821 ctggtggtca gcagcagcgt attggagtcg ttcgtgccct tgcagcgaat caagatatca
     5881 tcttgatgga cgagccattc ggtgcgcttg atccaatcac tcgtgattcc ttgcaagacc
     5941 ttgtcaaaga tttacaagag cgtttgggaa aaacgatcgt atttgtaacc catgacatgg
     6001 atgaagcgat taaactagca agccgtatcg ccatcatgag tgaaggccgt gtgatccaat
     6061 ttgatacacc agaaaatatt ttacgtcatc ctgcaaacga tttcgtagaa gagttgattg
     6121 gagaagatcg cctactacaa gccaagccaa atgcgacaag agtcggcgaa gttatgctaa
     6181 atacagcaat cacgattaca ccggaaaaat cattacaaga agcaatcaaa ctgatgaggg
     6241 aaaaacgtgt agatacgttg ttagttaccg ataattcgaa tgtattaaaa ggatttatcg
     6301 atgtagaaac gttgaataaa aaacgaggaa aagtatccag tgtcggcgac attttgaata
     6361 aacatgtttt ttacgtaaaa gaatccgcct atcttcgtga taccttgcaa cgcattttaa
     6421 aacgcgggct gaaatacgtg ccggtcgttg atgaacagaa caaagtcgta ggtatcctga
     6481 ctcgtgcatc attagtggat atcgtctacg atgtgatttg gggagaagat cccgcaacta
     6541 ctgatgttgc agagtcagct actcctgaag aatctaagga ggtctgacct atgcagcagt
     6601 tttttcatca gcacggttca gaaattctct caaaaggttt tgaacatctt tatatttcag
     6661 gacttgcttt gatcattggt atcatcattg ctgtaccgct tggtgtctta ctgacacgtt
     6721 ttccaaaagc tgccacgatt gtgattggct taaccagtgc cttgcagaca atcccttctc
     6781 ttgctttatt ggcattgatg atcccgctat ttggcgtagg taaattacct gcgattatcg
     6841 cactctttat ttattcatta ttgccgattt tacgtaatac gtacattggg atgaaaaatg
     6901 tcgattggaa ttatcgggat gtggcaaaag gcatggggat gactgagttc caatccattt
     6961 tttccgttga attgcccatt gctatgccaa cgatcatggc tggtattcgt ttagctgccg
     7021 tttacgtgat tgcatgggct acacttgcct cttatatcgg cgctggtggt ttaggcgatt
     7081 tgatttttag tggactgaat aattaccaac cagatctcat tttggcaggt acgatccctg
     7141 taacaatttt agcactattg gctgattggc ttctaggtct cttggaacac cggctgacac
     7201 cgatcgcact tagagaggag aatgaagaat gaaacgtata ctaaaaatga ccatggttct
     7261 tttctctctt cttatacttg cgagttgttc cttccctggt cttgccagca atacagatga
     7321 agacaccatt tcgatcacag gtggaatcac ttcagaagca cagatcttag caagtctagt
     7381 tgccggaatg gttgaacatt atacagataa aaataccgca atcatcaaca atctggctac
     7441 tacaactatc aaccatcaag cgatgatgaa cggagatgct tcaatttcag cggctcgcta
     7501 cacaggaaca gatttaacga ctacattgaa ccttcctcct gaaaaagatc cgaaaaaagc
     7561 gtttgctacc gtgaaagatg aatttgagaa aagatacggt caaacgtggt tcccgtccta
     7621 tggttttgaa aatacgtatg tctttcttgt tcgaaaagac accgcacaaa aataccatct
     7681 atctaaagtc agcgatctga aaaatgtagc ggatgaatta gtagctggtg tagatacttc
     7741 ttggatcaat cgtaaaggag acggttatga cggcttccaa gaaacttatg gcttctcctt
     7801 caattcgata ttacctatgc agattggact tgtttatgac gcagtcgaag caggaaaaat
     7861 ggatattgtt cttggctact ctacggatgg acggatcgcc agttatgatc tagtcatgtt
     7921 ggaagatgat cgtcaattct tcccaccata tgatgctgct ccagttgtag acaacaagat
     7981 attagctgat acgcctcatt taaaagaagc cttaaataag ttaggtcata ccatcagtac
     8041 ggaaaaaatg caggaattga actacgaagc agataataat ctagtggaac catcagtagt
     8101 agcacaacgt ttcttacaag aaaaccacta ttttgaaggg aagtgaagca attggataaa
     8161 atgaatgtct ttcaacaatt tttctattat ttccaggaaa atggctccta tatctttgca
     8221 caatttatcc gtcacttcct gatttctatc tatggtgtcc tctttgcagc agttgtcggg
     8281 attccggttg ggatcatgat ttctaggaga cgtaaattag caaactgggt gattcgtttg
     8341 gcgaatatca tccagacgat cccttctcta gccatgatct ccattttaat cattggatta
     8401 ggcctgggag tcaatgtcgt gattgttact gtatttttgt attccctctt accgatcatc
     8461 aagaatacgt ataccgggat gatccaagta gacaaaaaca tccttgatgt cggcaaaggc
     8521 atggggatga ctgctagaca acgactattc atggtagagt taccactctc tgtttctgtt
     8581 atcatggcag gcataagaaa cgcgttagtt gttgccatcg ggataaccgc gatcggtgca
     8641 ttcgtcggtg ctggtggtct tggagatatc attatccgag gaaccaatgc aacagagggc
     8701 acatcgatta ttttagcagg tgcattgcca actgcattaa tggcgatcat cacggactgg
     8761 cttttaggta ttttggaaag acggctagat ccagccagcc gtacatcacg ctgatgttat
     8821 tgtaaaaaac aaaagcttct gcgaccattt gttggtcgca aaggcttttt tagttctgtc
     8881 atttttaagg caataatcca ctctgattct tccctcctta tgggcaaaga gttgcttttc
     8941 ttagctattt atccattccg tatactccaa cttcatactg ccaacgggtt catatctgca
     9001 tccaccgact aagttttttt cttatccgct aaggcatttt gccaatccat tttcccatca
     9061 ctcgtcatgg aattcatacc acatgatctc taaatactcg tctgcttgtt ctttgaattg
     9121 ggcatcaatt cttacatcct ttggcgtggc tttcatttaa gccttttcca tcagtcctcg
     9181 attgttcgat gccttcagct gcatcatcaa aactggtgac tgccgtatcg gtaacttcga
     9241 tcatctttta tacaatcggg aacttgccat cctcatcctt ggctaaaaac atatctagcc
     9301 attccagctt ctcgccatgg gaggtttgcg gttcctaaaa ccggtacacg attcttatgg
     9361 actgcatccg tcgcaactag acttggcgat acgatgatcc ttcaccagaa gagcaccccc
     9421 gtaaaccaat tgatcgatat attgccaata agaaaagaca ttggcgtaaa agatatcgat
     9481 ccctcgtgat gcattgccgt tgatactgct attcatggcc gaaagcgcca cgactttaac
     9541 ttcttccgtt tgagtgacat tcatgttcgg caatgtcgct ttggataccc gctctgctaa
     9601 aggaacgaca ctggtattgt atttcgtatc tgtatctttt ccaaaagacc atgccaacga
     9661 atcctctggg aaccaataag acgactccgg ttgattgtcc attgcttttt tcaacatact
     9721 tttggctgtt tctcgataaa cgacatttcc aattgtcttc gcctcacttt ggcgataatc
     9781 gatggtcgtt gccactgcca acactaacaa cgatgctcca attttactga ttttccttgt
     9841 cacacttctt cctccttgtt ttacataaac ccgtcaacat tccattccta aactcaaaca
     9901 atgacccttc aaaggtggag atataggaac aaattaaaga aatcatgcaa taaatccaag
     9961 aaggttggtt ttagaacaaa aaaagagtct cctttttggg gagactcttt gttaactatc
    10021 taaaaaggtt ggctcttctc acttcactca taatgtgtcc aggcagaata aatttctaca
    10081 actttatttt cctcatcaac tttgtaaacc actcgatgct gtctattcag cctgcgtgag
    10141 tatctgcctt catgtgtagg tctcagcttt tcaaaagact gcgtgggcat atatggatct
    10201 tccttcaatg tctgaatcac ttcttcaaat tgtttcttta aattcgtttg cttgattttt
    10261 cttaaatcca ctttcgcaga gtttttgatt gcgactgtat agttactcat caaagagctt
    10321 cccaatcaat atcatcgata ttcgtaaagc tactgtcgtc tttttcgcgg tctcgtactt
    10381 tatctaaagt acctgtttgc tcaagaaaaa gggtttcttg aatcgctctc cagtcttcca
    10441 gcccaatcaa tacagcattg tttccactac gatcactaat aatctcgatt tcagtatgat
    10501 tttcatttac ctctttcaat aactgataaa aatttttacg agcagtgcta ggattcatcg
    10561 ttgtattagc cattgtctct cctccttttt ctgatataat tatagcgtac ttgattaagt
    10621 acgtcaaaaa aatacaataa agcacttaaa ttcagcaaac ttttcctctt ttttacatag
    10681 agaaaaagac ccctttttag ggaggatctt tgtccacgct cacttcttac tcctttacga
    10741 ctagcggtag ccaaacgtaa agtaattggt aacaacgtat catctttagc aaaggtcgaa
    10801 tccaaagcct gtgagtctat gcaacaacac caatacacga aggttcctcg ttgcagtgtg
    10861 acttgattga tatcttgaat aacctgagga tacgcacgaa agatttgtaa cggttattca
    10921 aaactgattt tggccgtcaa gtcttctact gcctcaaaaa tgattgcctc tttcgtctcc
    10981 tgtcattcat gttcactatc gatttgatac ttgcttagac cgttgggttt gtgaatcacc
    11041 aattaaaaat ggcactgagt tatggtaaca aaaaatggtc acttgacgag gctgagattc
    11101 ctacttatga agacacatgg cacttaagag tcgaggtaaa ggaagaagct gaaaatatag
    11161 gaggctgtac attatttggc aaaggattcg gaaattatga tcaagacatc aaaatcaaaa
    11221 tgttttacag ctaaatagga agaataataa catttcatac gctgttccca tctttaatgg
    11281 cttatagagg gcacctttag ctgagaataa gacaaaacag accctctagc ttcaattaag
    11341 agctagaggg tctgtttttg attttaaaac ttggtttatt tcttcaaatg gtagctatag
    11401 gtgctgacaa cgatattttc acgccaagac aatcgcaatc ttaaccgcaa acttgtttgg
    11461 ttatggagaa tattctgcca gcgtctgttt ggtacaaatt gaggaatcaa cacagtagtt
    11521 gtatagtttt gtttcgctgc atttttactt acgatatcta cataacggat aattggattt
    11581 tcaatcgagc gataagaaga gtggacgatt gaaaaacgga catctgggaa atgcttttta
    11641 aattctgctt cgatttcttt ttctttttcg acatcttcgt ctagcgaaac atgcatcgca
    11701 acgacataat caccgatcga acgtgcgtaa ttcaacgcac cgatatttac ccgtgtcaca
    11761 ttcccgacta atacaagaac ggtattcccg tcataatcat gtagttttgc atcattttct
    11821 aaacgaagct gttctgctac ttttttataa tgatcatgga tcttataaaa aatgaacata
    11881 acgattggca taatgatgaa gaaaggccaa atgtccccta accgatagac aaacaaaatc
    11941 gcaatgattg catatgagat gaacgcaccc gtaatattgg caatcgattt gcttaaccat
    12001 ctttttcctt cttttttcca tttgacgacc atccctgttt gagataaagc aaatggaatg
    12061 aacacgccga tagagtataa cggaatcaag cgttctgttg agccatggaa aataaacagc
    12121 aatacgatcg aacctagcgc tagtgtgata atcccattgg aatagcctag tcgatcccca
    12181 cgatcttgat acatgtgagg catgaattta tcttttgcca gattgtatgc aagaactgga
    12241 aaagcagaaa aaccggtgtt agcagcaact gctaaaatca gcgctgtagc aaattgaagg
    12301 acgtaataca aaataccatg tccaaagaca gctttaccaa tttgagacaa aacagtgact
    12361 tctttttcag gaacgatccc ataccaatag ttgataaatg tgatccccac gaagaagaaa
    12421 cctaagatca atgccatcat cgtcaatgta gcagcggcat tttttgctct tggcttctta
    12481 aagaatggta cagcattact aatcgcctcc accccagtta aagaggaaga accagaagaa
    12541 aatgctcgta aaatcaaagc gatagaaaca cccggcacag ccgctcctgg aagtgcagta
    12601 gcttgcaatg gcacagcacc tgtcacgata ttatataaac caacaacgat cagtaacgag
    12661 atcacaagga taaatgtgta caccggaaat aataggaacg atgcagattc cctcagccca
    12721 cgaagattca gcatcatcag tagcaaaacg attgtcacag aaatagcgac ttgatgaccg
    12781 tagagtgctg gcaccgctga tgtgatcgct tcagcacctg ctgagacaga tactgctact
    12841 gtcaacatgt agtcgatcaa taaagagcca ccggaaatca agccagcatt ctttccaaga
    12901 ttctcactgc tgacgacata tgctcccccg ccatgaggat aagcatggat gatttgacga
    12961 tatgatagcg ttaatgaaat caataaaata ataacaaaag cggcaatggg tagcgaatac
    13021 cagatggctg cagcagacaa agcgactaaa acgacaacga tttgttccgt accatatgca
    13081 atagatgata acgcatcaga cgacaataat gccaaagcag caaatcgggt taatttgtgc
    13141 tcatcatttt cagcagattt taaaggctta cctactaaca acctttttaa gtaatccaat
    13201 ttctaaactc ctttacacac aattttactt caagttttat agtcgaaaat ttatagtaaa
    13261 caagatgtta tagtacgctc actgacctta aaagtcaata aactttaaga aaataattgg
    13321 aaaaaaacag aaaaatatca agcgcttttt caaacgcttt aatacagcaa aaatctaaca
    13381 tttttttatg ggctttttct ttgaattcta taataaaagg atagaacttg acttagaaaa
    13441 tcaagttcta tcctttttga tatcttaact tttggaagag agatgtaagg ctgctttcta
    13501 cccatcattc ttactactcg aagcatagcg cagttgtcac aacctcttga taaacggtgt
    13561 ttaaaagctt gcatctgcgg taataagctc aaccaaatta aaaacataag aagctgtttt
    13621 tatttggtcg ttcttattac ttgatgcaga gcagcttttt tacaacctct tggtacacgg
    13681 tgttcaatca gctgaccctc ggtattagct caaaaaaacg gcaaaatatg aggaactatt
    13741 tttccatttt tttctctaat actcaggtca aaacactgat ttcacaacct ctttttttaa
    13801 tgaaaaatat ggctctcatg cgccgcttct ttgaatttcg tcattttttc ttcgatgaac
    13861 ggatagattg ccgttcctac gattccaaag acaacaaaca aaccgatcat gatccagatt
    13921 gctttcgtag catttggcca ataaattcct cctgcagatt cccgtaacaa gtttaccgca
    13981 tgagtaaatg gcaagaacgg attgatcatt tggaagaatt tccctgagac ttggatcgga
    14041 tagttcccgc ctccgccaga gatcgacagt actaagatga taatagcgat ccctttaccg
    14101 acattatcga ataatgcagc caagacatag acgatcatca tgaacgctaa ggcgatcaag
    14161 acagcaaatg acacgctgta gaccggattt tggacatcta ctcctagtaa gaaaatattc
    14221 cctaatgtaa cgatcaagct ttgcgcaatc gccattgtca aaaatgtcag catacgagca
    14281 acgaattgtt ctcgtttcgt aaaattgttt cggtctttct ttcctaaata gtattctgtc
    14341 gtagcaacac ttgaaagaag aacagcgccg acccacagac atagtgcagt ataaaacggg
    14401 gtgctagctg aaccgttatt tgcgattgga taaagcgcat tggtttgtag ctcaacaggt
    14461 tgtgtaaaga agtcactttc ttttgttgcg tctagtttca ataatttgat gacttcgcct
    14521 agatcgactt cttgttcgcc ttttcggatc gcgtctgctg ctttttggat accagctttg
    14581 atatttggcc agtcattgtt gatcaagtcc gcagcagcgt tcacagctgt ttcgacttct
    14641 ggcatttttt catttactgt tgtcaatgta ccggtgattt cattttttag tgctggccaa
    14701 tcattttgga taaaatcagc ggccaatcct aatttttgct gaatgatcgg taattcattg
    14761 ttgtataggt ctgccccttc attgatgcca ttgacgatgg tatccatatt tccattcagc
    14821 attgtattgg catcatggat ttcttgtcca attgcaggca tttctttttg atatttttct
    14881 aaaattgcca ctgcattcgt tacagttgct ttcgttgagc tgagaagcga atcaaagtcg
    14941 atctggtccg ctttgctcag tgcgccttgt gcagtcccaa tcgtatcgat gacttggtct
    15001 agtagagaag tgacagtgga actgatttga cctgtattga taccagcaat gatgccattg
    15061 atattattaa caacggcatc aatttgatct agcaattcat caatagcctt cgtgtcttca
    15121 ttttgaacag ctgtgttcaa agcagatagg cggttttgca gattgcttag caagggtttt
    15181 aattgattta gctgatcaat aattggctgt aatttcgtat ttccagcact tgtttgcaat
    15241 ttagtcagga actcgaccag tttatcgatt gcattttgtt gttgagatat tccgtcaata
    15301 aagccgttca atacattgct gatgtttttc ttttcatcat cagtcagctt accatcttca
    15361 atagctgttc ttacagtatc tattaacgaa cctgtcgttg ttgccacttg tccaattgac
    15421 ttcaatgtaa catctacact gtttgtgatg cttggtatcg catctttcag ctgagaggca
    15481 cctgattttg tttggtttgc tagatcactt gcctgatttt ctaaattttt cacatctggc
    15541 aagatatctt gtacttgttg gatgatcgtc aagccttgtt ttgcttcatc gattccttgg
    15601 ttcattgtct cttggatcga agcaaaatcg ccgtcgatct ctgctagttg cttaccggca
    15661 ttttggatct ctgggatttt ttcttgcaaa tcaaggatga tttttgcttg atctttcaac
    15721 tcaggcattt tgtcattcaa tgcaacgact ttttcaccca tctcatctac tttagggatg
    15781 taatccacga attcatttgc tttattcagt ttttccttga tttcaggtag ttgactttgt
    15841 aattcgagga cttgttttgt gtagccgtcg atcgtgtcca aattttcatc tgttgataaa
    15901 atcatatctt taactttatt gatacttact aggttcgtat cgatgtcgta acctatctca
    15961 ttgaatactt tcaataaagt cgaactggct gttttgataa attcatttgt gatttgcgat
    16021 tggatcgaac ttgctccttt atcggtgatt ttcggtgcaa tcgcattgat tttttcattt
    16081 accgtatatt cgatttttgg tttcttgata tcaccactag taaaactcag cagatcttct
    16141 gagaaatcct ttggcaagta aatacctgcg taatactttc cggaacggac tccgtcttct
    16201 aattgttctt ttgaatcaac gaattgccat cctaattgtt tatttttatg aagcgattcg
    16261 atgacttgct ccccaatggc cacttcttta ccttgaaact ctgctggctt gtctgcactg
    16321 taaacagcaa ttggcagctc tccagtattt ccatatggat cccataatgc tttgatgtta
    16381 aaccacgcat atagcgaagg gatgatcatg atcgcgatga tcagaagtgt tgcaatcggg
    16441 tttttgaaaa tccgtttcca gtcaagcaaa tataatcgaa gcgtattttt gattgactgc
    16501 atagttctcc cccttcttct tttttcagct gaattacttt accatgtatt tcatactttt
    16561 ttcaatcggc ttttttcaaa aaagtcgaat ttttggacat atcacggcaa ttgtctcatt
    16621 gtaatctctt acatattgag aaataattaa ggattacagc ctttacttta tcatgaaata
    16681 aaagggaaag ataattttat gccaaagttt ttatctatta tacgtatacg caaaaaagct
    16741 aaaacatttt ttgtctttgc ccctacaaaa atgtttcagc ttttttcgtt ttttattcct
    16801 gtatttctta tttttcgaat gacaggatga ttttcccagc aggaggatgt ccttcaacta
    16861 gctgatgtgc ttggatgaca tgcttaagtg aagcagacag attcattgaa ataaaaacat
    16921 gaaaggctcc tttttggtag gctttgatta tgccagcaaa ggcttctgca tctagatatt
    16981 cctttcttgg aacaaacgat tcataaaagc cttccttttt ttgccgcaaa tctagatctg
    17041 ggaggtcatt taatgcaaca tacctgccgc ctggtttcat gatctggatg ccggtttctc
    17101 ctttgatact tcctttcgtc gcatcgatta caaggtctgc ctgatcagca aattgcagac
    17161 ctggatttgt tttatcataa gcagcaaatg ctgaagctcc taattttttt accttttcct
    17221 catttttact ggatgcactg gttaagattc ggattccctt ttcatggaga agctggatca
    17281 gacttgaccc gaccgcacct gatgctccta gtatcattac agtatctgtc ggctggattt
    17341 ccgtcaaatg gttgattaga ttataagccg taattcctgg cgtgaccatt ccggctgctt
    17401 cttcccaact cattttatct ggaatttttg ctacttttgc tgatggcaac acgacttctt
    17461 ctccgtaagt cccgccgact gcatggacag ctactcgatc acctacatga acagttgtga
    17521 catctgaacc aatctctgtc acaataccag cgccatcatt acctggtaca taagggaatt
    17581 tcaatgttcg tatctctttc atttctccta aacgcaaggc aacatcataa ggattcacac
    17641 tgaatgcttt tatttctaca cgaacatgtc cagaaccgac ttctctggga tgagcatcga
    17701 tttcttcaaa aacatcttgt gcttctccgt aaccgttaat tgcaaatctt cgcatggtca
    17761 ccatctccct ttttctttaa gtttacgttt atgtctttat tttgacaaaa aaaatgtctt
    17821 ttcgttttgg cgcatgattc aaaacaaaat agttgtcttt tttcgcgtaa cgtgttaaat
    17881 tatttatcta aaagcgtaaa gattacgatt tacgctaata aaaaataatt ggaggaattt
    17941 gttgatggct aaaaaatcaa agattgccaa agcaaaaaaa cagcaagaaa cgattgaacg
    18001 ctatgctgct ttacgccagg aattaaaagc gcaaaaagat tacgaaggct tgcgtaaact
    18061 tcctttagat gctcgtccag aacgattgaa aaatcgtgat ttaatcgatg gacgccctcg
    18121 cggatacatg cgtaaattcg ggatgtcccg tataaaattc cgtgaattag cacataaagg
    18181 tttgattcca ggagtaaaaa aagccagctg gtaatccaag tctggctgta atgtaaagga
    18241 ggagggcgca tggaccactc cttggatcat caaaaaagat acgttcgctt cttctagcag
    18301 aaatgctggt actagaagca gacgtatctt tttttacgca cagctggtcc ttttttatat
    18361 gtaaaggctg catggttact tcgctatatt tatttagtaa caggagtcag tgcttcgata
    18421 cccacactaa ttttcaatgc tcggtcaact tctgccataa tgggtcgatc caaatggcag
    18481 accttttctt tcaatcttga tttatcgatc gttctgatct gctccaacag gataacggag
    18541 tctctttcta ttcctgttcc agtagctttg atcccaatgt gagtcggaag cttaggttta
    18601 gccatttttg ccgttatggc cgcaacaatg atcgtcgggc taaaatggtt acctagatta
    18661 ttttgaataa ccaaaactgg gcgtgttccg ccttgctccg atcccacgac tggagaaaga
    18721 tcagcaaaat aaatatctcc tctttttacc ataggatcct ccttattctt gatattctct
    18781 tggtatccgc tgtcctaatc cgcaagccac ttcatagtgg atcgtaccta attgatctgc
    18841 tatatcctgc atagtgattt ctttgtctgc atttttgccg ataagcgtca cttttgttcc
    18901 gacaggaaat tcttgaggca atcggatcat cagttgatcc atacatacac gcccaatcgt
    18961 ttcacagtag ttcccttcta ccaatagaga aaaaccctgc atctttcgtg gccagccatc
    19021 tgcataaccg atcggtatcg ttccgatcca ctctgcttca ggagtgatat atgtttctcc
    19081 atagccgatt ccttcacctg ctgggagttt tttgacttga atcaattcag aaaccagctc
    19141 aagtgctggc tgcaatggat aaacttccgg tagtgcgtgc ccagagggat taagtccgta
    19201 cattgctact ccataacgaa tcatattgcc gattgtttca tcatgccata aagccgttgc
    19261 actattgctg acatgtacat atcgaggaag ttcgggaagt tttttcagca cttctataaa
    19321 gcgttctttt tgcagattcc aatatgtatc atctgcctga tctgctgtag aaaaatgggt
    19381 aaataaacct tcccattcta atacacgact ttctttgatg aatgcagctg cctcttttac
    19441 ttcttcaggt gtacaaaatc cgatacgacc catcccagtg tctgctttta gatgaatcga
    19501 aagaggcgtc tctagcttac ttttctctaa aacagcagct gctgcttgca accattcccg
    19561 tgtacctgca gttactgata aatcatgagc aacagctaca gtcagtgcgt cgtaaggaac
    19621 catattcaag ataaggatgg gctgagtaaa tccagcttct ctcaactcga ctccttcatc
    19681 taaatttgaa acacaaaagc ccgaagctcc acctgcaact gctgcctcag ctgtttcaat
    19741 agctccatga ccgtatccgt tcgcttttac tactgcaaac aattcttttc cttgcggaag
    19801 tcttgcaact tcatttgcta cattttcagt tatagctttt ttatgtatca ctgctttcgt
    19861 tgggcggtgc caagcgacaa ccatcgtttt tccttctttc tagtcttcca aaataatttg
    19921 tgcaaaagct gtttcatttg tatgagtaat actgacccat acattgcctt catgaggtga
    19981 acgcgtcaca acaggctgac cattttcatt ttttaaaatc tcgacatctt gaaaggtaac
    20041 ttttccgatc ccagttcccc aagctttaga gaaagcctct ttgcaagcgt aacgcccgcc
    20101 taaaaattct acttggcgat gaaaaggcaa ggactggaat agtttcattt catcagaggt
    20161 caagattcgt gcaatgaatt ttggtttttc ttcgatcagc cgagtgatcc taggaagttc
    20221 gactgcatcg atcccaattc cttttatcat atgcttttct cttttcaata aatttagaac
    20281 actctcattc taccaaatta taaccatttt gccacaaata agtccacaaa taacaaaaat
    20341 gaaagtttct cttaaaaaag agaaactttc attttatgtt taatcgttat ttgaacggat
    20401 tacaaatccg cgttttttat cattgtgacg tttaccgcca tctttttggc tgttattatt
    20461 tttgctataa ccgccttttg atttgtttcc acgataatta ccgccgtcac gacgattacg
    20521 gttgtttccg ccaccacgtt tgccattttt attgtatcct tttttctgca ttggcaatgg
    20581 gcgttctggt gtgatcttca ctggcactgc atcagctgga tcttttgctg tcgtttttag
    20641 caaaagtgca actaaatctt gtgcagaata ttcttctaat aatttatctg ctgtttgcag
    20701 gtatttatct agaccatttt ctgcaagttt tgtttcgatt tgttcaactg cagcacctaa
    20761 ttgacctttg aatgcttctt tttcggttgg tggacgtaaa gtcgtcatcc gttttttcgt
    20821 caagttttca atcacatgca agtagtccat ttcatttggt gtaacgaaag tcacagacat
    20881 accgcctttt ccggcacggc ctgtacgtcc gatacggtga acgtagcttt ctggatcttg
    20941 agggatatca tagttgtaca catgagtgac acctgaaata tctaatccac gtgcagcaac
    21001 gtctgtcgct acaaggatat ctaaatgacc gctcttaaat gaacgcaata cgctcattcg
    21061 tttttgttga gacaaatcgc catggattcc ttctgcacgg taaccgcgtg cttctaatcc
    21121 gcgtgccaat tcgtctacac ggcgttttgt acgtccgaag acgattgtta attcaggtgt
    21181 ttgaacatca aacaagcgag tcatgatatc gaatttttcg tattcttttg cgcggacata
    21241 gtattgatcg atcaagtcag ctgtcatttc tttcgctttg atcttcacat gatgtggatt
    21301 tttcatgaat ttaacgccaa tattcttgat agctggcggc atcgttgctg agaaaagtaa
    21361 tgtttgtctt tgatcaggaa cttgtgaaat gattttttcg atatcttcca agaagcccat
    21421 attcaacatt tcatctgctt catccaaaac taatgtttga acggttccaa gtttcaatgt
    21481 gtggcggtta atgtggtcaa gcatacgtcc tggtgtaccc acaacgatat gtggacgatc
    21541 tttcaagcca cggatctgac ggccaatatc tgctccgccg taaacggctt gtacacgtac
    21601 ttttttgtcg cgccctaaac gatataattc ttcttgtgtt tggatagcta attcacgtgt
    21661 tggtgcgata actaaacctt gtaattcgtg acgatctgga tcgatttttt ctaacattgg
    21721 taatccaaat gcagctgttt tccctgtacc tgtctgtgct tgtccaataa cgtcttttcc
    21781 agctaatgct agtggaatcg tttctgcttg gataggcgtt gcttcttcaa aaccagcgcg
    21841 ttcgactgat ttcaacaatt ctggtgataa ttctagttct ttaaatttca aatgaatcct
    21901 cctatatggt tgttaactga ggatgtagct tttttgctac cccgtcgatt tgttcggtca
    21961 aagtgtattg tttctttttg acagtggcca cgcacgtttt tatccattct tagcccctgt
    22021 ggcaccaccg aaaaatcact ttgcgaagtt taggcattag aaaagccaaa cgggtcgcgt
    22081 tcagctttat cattattatg acatctggct tcaacaagtt gtttctatgg caaaaccagt
    22141 ccaatttctc ttcatctttc ggcaataggt tattctaaca tagtttttcg ttttctgcaa
    22201 gtttacagat ttttatttca tttgctgaag ttgttctacc acttcaaata agcccatgct
    22261 gttgctgcct tttagcataa tactatctgt tgctgcaaga ttttgtttga tagcatcaat
    22321 caatttattc ttttcttttt caaaatagaa cacaggtagt tgggggtatt tttggttcaa
    22381 ttcttctttt agtgcatgca tttgttcccc ataaaggaaa atcatggcga atttctcagg
    22441 atcgatatgt tcactcattt gcgcatgcat atttaaagac tctttcccta attccagcat
    22501 atctgctaac acagcgattc tgcgtccttt tgttggcaga tttttaaaag tatctaatac
    22561 cagtcccatg gctgttggat ttgcattgta gacatcgctc aaaatagctg caccatttcc
    22621 tgccgttagc cattcggttc tgttttgagt gatagaaaca tgggcgagcc cttggcgtat
    22681 ctcaggtatg gacagtccaa agaagcgccc gatcgtatac gcaatcaaag cattcgttac
    22741 attataactt ccaggcagcg ggatcgtaaa tgtttctcct tctactacaa aatcggtttg
    22801 tgcttttcct tttgctgtca cttccgcatg aacatcgcca tttccaatac cgaatgttgt
    22861 gactgtttgt actaatgttg ccgttaaagg tgtcagcaac ggttcatctg cgggcacaac
    22921 gagtaatccg tctgatacaa gtccgtcagt gatttccatt ttggctttag cgattccttc
    22981 tcgcgaccca agattttcta tgtgtgcttc accgatcatt gtgattgctg cagcttttgg
    23041 ttgagccata agtgaaagtt ccgtgatttc attggcgtga tccatgccca tttccaaaac
    23101 gatcttttct gtatcttggg gcatgtgcaa aatcgtgtaa ggaagaccaa tattattgtt
    23161 gtaatttcct tgcgtcttat atgttttgta tttttcagac aagacagacg cggttagatc
    23221 tttcgtggtg gttttaccat tgcttcctgt aattgcaacg acttctgcgc ctactttttc
    23281 caagtaatag gttgccaatt gctgcatggc tttcaaagga tctgctacag gaataactgg
    23341 aaaatttttt ggagcttgtt caacaggcaa gctccaaaat gaagcagatg ctccgtttgt
    23401 gatcgcttgc tggataaatt catgtccatc tcgcgtacca gcaagaggaa cgaataaact
    23461 tccttcggtt atcttccggc tgtcaaattc gacattggta atctttgtca gtgcagagca
    23521 ttcgctgccc acggcttgtg caatttcttg tattgttaga ttcattgaga tctctcctaa
    23581 aaaaggttgt gaaaaaagcg tttggcctaa acggcgggtt cgcatgactc tcacttccaa
    23641 gtaaatttct ctattcgtat ttagaagcta gaatgaacgc ggttacgcaa tcactccagc
    23701 ctcttcttta tttctcttta ttcatttttt tcaaaaaaag attgacgctg atggtatcga
    23761 ttcattccta actggatcag ttcttcaatc aaatcaccgt atttcaagcc catattttcc
    23821 cataagagtg ggtacatact gaactccgta aatcctggca tagagtttaa ttcattcagg
    23881 aataattcat ttttatttgt caaaaagaaa tcgcaccggc tcaatccgct tccacctaac
    23941 atcgtgtaag ctaacttcgc gtactcttgc gctttttgat aaacttcttc tggcacttcg
    24001 gctggaatct gcatttcgat tttattattg atatattttg cttcataatc atagaatgct
    24061 acgtctttta cgacttcacc aggcaaagtc gtccgaacgt cttcatttcc taatacagca
    24121 acttcgattt cgcgcgcttc aattccttgt tcaacgattg ctcgagaatc atactgatag
    24181 gctgttgcta aagcattttg cagctcttct cggttttctg cttttgtaat gccgacacta
    24241 gaacccatat tcgccggttt gacaaacatc ggataaagca aagaaccttc acattgatca
    24301 aatacttttt taggattttc tttccattga ttcttaagta ctggtacata aggaacttgc
    24361 ggcacaccag cagcttgtaa aatatacttg gtcatgattt tatccatggc acatgcactg
    24421 gtcaatacgc ctgcgccgac ataaggcata ttcaatgtct ctaagaagcc ttggatcgtt
    24481 ccatcttccc cgtttggccc atgtaaaact ggaaaaacga tggctccttc ttctttgatt
    24541 tcgcccggat tgatcacttt tcctgtaaag ccttcctctg tctgtccact tgggtcccat
    24601 gaaagatgca agacatcttt gctggcaggt ttttctgtta gtaatggacc tttgacccat
    24661 tgtccttctt ttgtaataaa tacgagttga acttggtaat aattataata aatggcattt
    24721 aaaactgaaa atgcggaaag aatggacact tcatgctctg cgctgcgtcc gccatatagt
    24781 aaagtaatct tcaaaagatt gtcctcctgt attctaaatc gttcagtgat ttactctcat
    24841 attttagcac aaaacaaaaa taaacgaata acttatttat aaaaaaacga gccagcttct
    24901 aaaagaacga gcgtcattta ttttttgtca gctagattat ttgtcccgtt ttttataaaa
    24961 agttatgata aagaaaagga gggatgatga tggcgcaaac aaatccacgc agccgcttgg
    25021 cagaaatcat tcgagtgttc atcaattaca atgtagtgcc aaactttgta cagcaaaaaa
    25081 atcccgaaca ggtaaaaaaa gcctttgaag aactaggccc caccttcatc aagataggac
    25141 agatgctttc tgttcgagaa gatttgcttt cttctgcctt cactcaaaca ttcaaaacat
    25201 tacaagatag tgtacctagc gacacatttt caacggtcaa aaagacgatc gaaacagaac
    25261 tttctctttc attatcagat atattcgatg atttttcaaa atcgcctttt gcctctgctt
    25321 ctatggggca ggctcatcgt gccaagctga agaacggtga cgcggtagtc gttaagatcc
    25381 aacaccctaa catcgccgaa gagatccgac ttgatcttca actatttgaa cgagcgatcc
    25441 ctttgatcaa atacattcct gaaactagcg tagttgatct aaaaggggta ctccaagaag
    25501 taaaacgttc cttgatcaat gaaatggact tcttaaaaga aagtcaaaat ggcgaacaat
    25561 tctatcaaaa gaacaatggc tggaaagaga tccgcagccc caagatttat gacgcttttt
    25621 gttcgaaaaa agtgatcgtg atggaagaaa tgtctggtaa aaacttgaat catttaatga
    25681 acgccgaaaa caaaactgaa acttttatca ctggaattca aaacaagcag ctgaaacaag
    25741 aagttgcaaa gcttttagtt gagaacttta tgaaacaggt gtttgatgat ggttttttcc
    25801 atgctgatcc tcatccaggg aatctgctat tccatgtact aacaaaagaa gaacagacac
    25861 aagctagccg aaaaactgaa acagtccatg aaaaagagtt cggttccttt gcttttcgag
    25921 cctccacttc tgcagaagac cctgttgcgc catataccat caactacatc gattttggta
    25981 tgatggggca tctttctgca ggacttaggc aaaaactcac acaagccgtt ttagcgctgt
    26041 atacgaaaga tgcttatcga atcgaaaaag cggtccttcg actttgtcag caagaaggct
    26101 cgtttgatga aagtcggttc caccaagaac tgacaagttt tcttgaacag tattatgatt
    26161 ctcctattga tgaaatcaac ttgcaagaag tattcacaca tgtcgtgacg atttgtcatc
    26221 aaaataatct gcagttcgat cgtgatatca cgcttttgct gaaagcattt gggacattag
    26281 aaggggtgat ccgcgtcctc gatcctgaag tttccctcat ggaagtcgct tcgccttttg
    26341 ctcaacatta ttttctcact catctggatg tagaagatac attgaaacaa agcggattag
    26401 acttattgga agggatgaag gctgcgccta agatccctca acagctccac catctattag
    26461 aaatgtggac ttcgggacaa ggaaaagtca atttggaatt gaaaaagcaa gacaaattat
    26521 tgagcaggat cgaaagtatg atcaatcggc ttgttttcgg tatgatttta gctgcattga
    26581 tcgtagggtc ctccctgctt gtccaagcag cacctgtaga aaacgcagag gtcgtttcct
    26641 tattagggat atttacgtat gctattgccg cattcgtcat tatttttttg gcaatcgatg
    26701 cattgatcca aatgtataag aagaggaaaa agtagaaaaa ggttgtgaca gaaagaatca
    26761 gcttcaagaa ataagaaggg gttttacata aattgtttct caaatttttg tgaaaattcg
    26821 gcttattttt cgaagaggct acttctgaaa caccgagaat aaaaaatgtg aaaaagctgt
    26881 tctgcaccca gtaataagaa caatcaaaaa gggaacatgg aacacattta tcctgtttcc
    26941 ctgttccctt tttgatgtat tcgagcataa ataaaatacc ttcgttacct actatcttct
    27001 gtacttggta aaccttagtc aattgccttt tttaacgctt gacgaaaaga catatgatct
    27061 ttctccgtaa agggttttgg accctttgtc cttatgccag cttttcttaa ctttccacta
    27121 tgataacgtt gttctagaag cgtgccgata ttcgcttcgt caaattcttt tccagagtga
    27181 tggaacactc gcacaccctt cggcaataca agtgaagcta aaccataatc ttgcgtgatc
    27241 aaaaagtcat tcttcttaac cataccgacg attttataat ccgcactatc agctccgcgg
    27301 tctacgtaga cgatttggac atgattagcg tattctttgg tcgtataatg gtccacactg
    27361 gtcacgataa ccacgtcaag accataggat tctcctaatt ttatgacatc ttcttttact
    27421 ggagaaccat ctccatcaat gaatagcctc attctttttc gccttcttcc ataaatgata
    27481 aacagttact aatccgctca gtaaaagacc aacattgatc cattttgcca agataagcag
    27541 ttgtacactt tctatcggca ctgattcatg gttcagcgtg atcaataata gatggattgg
    27601 ctctcccatt tcttttgcta aaacaaacaa aagcgtcatc aataaaaact ggtggatcac
    27661 actcccttta ttttgagtag tatacttgac aacgatccgg cgatccattg gaatagctgt
    27721 atcagtttga taatattcct tatttagcca gctgcctgtt actacgtgcc agaaactaag
    27781 cagaaagaca cctatcgcaa aaccatttgg aatcaagagg accaataaag aagaactcag
    27841 tccaagcaat tgcatagcca atggagacca tgttaaacgt ttcaaaatcc atggtccgac
    27901 tatttttgct gcaatcaagc ctgctgcata aggcaaatac ataaaaatcg aaagtttttc
    27961 taatccgtac agtatgccaa cataaaaact gctaaataaa aaaaggaaat tccctagcat
    28021 tccatttaga aaagtaaaca gatttagcca gcttggcaac ttccattcct tcttctggtg
    28081 tcctaaaaca aacacgaaca agatgaacag cagcaagaaa ccataaatag caagatcaaa
    28141 aagcctttca tcgaataaca atcgggcatt tctcaaaaga aacaataagc caaagaatat
    28201 caaaaataaa acaagctctc gcgtagctac cagatttctt ttgatgtctt tgaaatccag
    28261 ttcgtaacgc ggataatgac taaccgtatg gtaggccatg acgtaaaaaa gtgtataaat
    28321 agtaaagctt gctgcaatct gagtcggtgc aggcaactca atcgcttgat acaggagaaa
    28381 tagccagata gctgcaaata agtacttttt cccatccata ttgataaaac cttgcgtctt
    28441 ttcgtgaaaa ttgaccgtgg tattagcagg tacaagccat gctgcactca gccctagcaa
    28501 aacacttgaa aaataaaata aaggaaaata aaatacgcct aatattccaa gcaatgaacc
    28561 agcaccacca atcagcagag cgatcatcag caacgtgtaa ctatctagac caaatcgtag
    28621 actgttgact aaaaatagtc cagtcatccg aaaggcataa aacagcgtaa atggcaaaat
    28681 cgtcagccaa ttatgcgagt ctgttagtcc caaaaataaa agataaggaa taaaaataac
    28741 cgtgttactt aagataaagg gcgttgtttg tgtaaaatta cgcccaaatc gtttgagttt
    28801 ttcgaacatg acaatggctc cttaaacagt cttataacta atctgctctt cgattttagc
    28861 attaaagggg aaataaacca attttacaga atatattctt gtaaaaaaaa atacagaaag
    28921 aagcataagc ttctttctgc atttttacgg tacttgatta ctcaagcaga caagcccttc
    28981 tccacaaaag tcttgcccga cacccctgca tccggcaggt gataaagata gaaatcaatt
    29041 atcctgtgcc tccaaaatca attaggagct ctgtaaacca gagtccttct cgacccgtcg
    29101 cataaattta ctttaacaca gttcctatgt ttatgcgaga gcttttttca ttttataatc
    29161 gaccatttga aatccttgac cgatatctga aataagttct tctgtaatct caaatccttc
    29221 atgcttataa acctcgattg cttgatgatt cccctgattc actcgcaaaa agatctctct
    29281 ttcctttgtt tgcgcagcaa gttcttcata ccagcgaaaa actgaagaag tcaatccttg
    29341 accacgtact ttatttagca aatataattt acttaaataa atttttccat ttatctcttc
    29401 ataagcagtg taaccgacag tcttttcttc gctttttaat aaaaagtagg acacaccttc
    29461 tgtcagttct ttttgaatct gtttttttga ctgataagtt ttcagcatgt aatccacttg
    29521 tttttgacca atgatcggca caaaagcttc cggccatact tcttgtatga ctgccatcag
    29581 ctcatccagt tcattttctg ttacttttat tttttccata cacccacgtc cttttttaca
    29641 ttataaatga ccttgcgctt gatggaataa aaagagcatg atcatgctat acacacccac
    29701 cgaccatggt ttcaagaaaa tcacaatctg cataaattcc ctaaaagata atttcgtcaa
    29761 accagctact agacagatca agtcgtctgg cgcaaaggga agcagcaagg taacaactag
    29821 tactttttta atatctttcg tttttcttgt cagcaattca tcaaatttct gatacttttt
    29881 tggcgataaa ataagctgga caaaggctac gccatagtaa cggacaagca aaaatccgat
    29941 tgcttcccca atcactaggc caatacaact atataaaagc ccaaatccat tgccaaacat
    30001 cagcatgccc gctactgagg agatccctcc cggcaaaata ggaatgatca cttggatcac
    30061 ttgcaacaga acaaatatca tcactgcata cttgccaaat tgctggataa acgcttgcaa
    30121 agacgtgctc gatcgaaaaa tcccatgatg ataagcataa agaatcaaca caaagaaaaa
    30181 aaacagacca ataatgggca tgatctgaag acatttttgt aaaactgatc cttttttaga
    30241 catctatatt ccccacatct tttttcattt attaaaactg cctccttagg gggtgcgaca
    30301 aaagagcttc ttcaaaaaaa taagtcgaaa tctcacacaa attcgagaaa taattttcgt
    30361 aagtttcttc ttatttattg catctgacca cttctgttac aacctctcgt taaacggtgt
    30421 tcaaaagctt gcatctacga taataagccc aacccaacga aaaacgtgag gagctgtttt
    30481 atttggatgc tcttatcgct tgatgcaaag acagcttttt tacaacctct acttcacggt
    30541 gctcaatcag ctgaccttcg gtattagctc aaaaaacggt aaaatataaa cagctatttt
    30601 ccgtttcttc tttttaatac ttaggtcaag atgctgtttt cacaacctct tgttataaat
    30661 aaaaaaacag ttggctggtg actgttcaaa aagcagaatc cttgcttttc aaacggtcgc
    30721 agtccgttca actgtttcgc tgcgcactat ttatggttta agaaactagc tacttttgtt
    30781 gtaatcaaat caatagcaac gtggttttct ccgccttctg gaacaatgat atccgcatag
    30841 cgcttcgttg gttcgataaa ttgatgatac atcggtttaa caactgtcaa atattgttcg
    30901 atcacggagt ccaatgtgcg tccccgttct tccatatccc gtttgatacg acgaatgata
    30961 cgaatgtcat catctgtatc tacgtatact ttgatatcca tcaagtcacg cagacgttcg
    31021 tcttctaaaa tcaggattcc ttctagaatg atcacttctt ttggttcctg aatgatcgtt
    31081 gcctgacttc ttgtatgcgc tacatagtca tacacgggtt tttcaatggc tttatagttc
    31141 agtaagtcgc ctacatgctg aatcagcaga tcattatcaa aggcaaatgg gtgatcgtaa
    31201 tttgtgttca atcgttcttc aaaactcaaa tggctctgat ccttgtaata agaatcttgt
    31261 tcaagcatca tgatggaatg gtccgggaag ttgttgaaaa tcgcacggct gacacttgtc
    31321 tttccgcttc cggatccacc cgtcacacca atgataattg gcttgctttt tgtcattgac
    31381 taaaacctct ttcgtatccg ttttttcatt ctaatctatt atagcgaaaa aacacgaaaa
    31441 tgctatattt ttctgaaaat taatcctttt caatttccaa taatgaataa agttcctcta
    31501 atgaatcgat tcgataagta ggctggattt cttcgatttc tggtaattta tttctattga
    31561 accaaatcgt atcgatccct gccatatttc ctcctttgat atcagaagtc aaagaatcac
    31621 caatgatcac agtttgcttt ttatcaaaat taggaatgtg ggaaaagaca tgatcaaaaa
    31681 attctttcat cggcttttgc gctccgacag cttctgagac aaagatatcc ttgaagtaat
    31741 caagcattcc tgcatctgtt aatcgccgat gttgcgtgtg cgacacaccg ttcgtcacaa
    31801 tataaagatc agcatgcgga tggacctttt tcaggatttc tagacttcct ggaacaagat
    31861 catgaccttg gttcaagtag tggcggtagc gtttttccat ttgatcacta tctactgttt
    31921 tcccaaattg ctggaaaagt aacccaaaac gactgcctat tatttcttct cgtgatagtc
    31981 tgcctttttc aaactcctgc cataaatgat gattcaaagc cttatagctg gtttcgattt
    32041 ctggtgtcaa tgtgattcct tgttctgcaa acaaagcact taaagcattg gcttcagtta
    32101 attgaaaatc taacaatgta tcatctacat cgaacaataa agttttatat ttcatctttt
    32161 tgacctccta tttaaaataa atacttaact ttttcatctc tgctaatgtc aacgatcgat
    32221 actcgcctac tcctagctgt tcatctaatt ccaatggtcc catgcttagc cgtttcaatg
    32281 cggtcacttt ctttccttgg gctaagaaca tctttttgac ttgatggaat tttccttctt
    32341 ggatcgtcaa taaaaccgta ctttggtgct tgtctgcttt gagtattttt aaatcagccg
    32401 gcttacattg atacccatca gcgaatacga ttccattttc aaatgctgtt acgtctgccg
    32461 gtgtcaccct ttcgttcaca actacttgat aagtctttga aacctttttt tctggaagta
    32521 gcatttcata tcccaattgt ccattcgaag tgatcagcaa tagtcctgtt gtatctctat
    32581 ctagtcttcc cactggatat aatggttgga aacgatcggc acttgcaatc agatcgatta
    32641 ctgtcgtctt ttctggatct gatacagctg taacagcttt ttcaggttta ttgagaagat
    32701 aataggactc atttgtttct aagtgttcac ctgctacttc gatttgatgc agtaaactat
    32761 ctacatttcg attttgtcga aattcgactg tgccatctat catcacttgc ttcaaagcaa
    32821 acagccgctt catttccttg cgtgtcgttt ctaatttttc ttctattaat ttatctaaac
    32881 gcatgctgtt tcctttctta ttactactaa ttaattatac cttgaatatt cttgcttgca
    32941 aatacatcat catagttatt ccttgactta attaacagtt cacgttacgg ttaaatagta
    33001 aagtgaattg gatttctcat acacagcgta atgacctggt ggagaaaagt tgaatcctga
    33061 ctttctaagg ggagatgcgc gcatctctcc ttttgcgctt ttttaagcgc cctgccttcg
    33121 atgatgtgca cgagattagc ctttagaatt cacttaaata aaatctgtcc gttaccaaag
    33181 cagacagtca cattttaagg aggaattttg gttatgtcag tatcattaga agtaagtaaa
    33241 agagaagtac gtccccgttc attaagaaac aaacttcgtc acgaagggaa agttccagct
    33301 gttgtttatg ggtataaagt agaaagtacc cctatctcat tcgatggttt gacactaagc
    33361 aaattgttgc gtgaacatgg agcgaacaca gtcatcacga tggacatcga tggacaaaaa
    33421 gtcaatacat tggttcacaa gatccaaacg aacactttta ccaatcattt cgaacatgca
    33481 gaatttctat ctgtaaacat gtcggaagaa acagaagtcg aaacagaagt cgttcttgtt
    33541 ggcgaatcag tgggtgctaa atctggtgga gtgttagctc aaaacttgta tacagtagtc
    33601 gtatctgcta caccagacaa attgcctgaa cgaattgaag ttgacgtgac aaacttggaa
    33661 ttaggtgatt ctattacagt agccgatctt ccaaaaaacg acgattacaa attcgtgacc
    33721 gacagtgaag agcaaatcgc tgctgtagtc gctcaaagtg aagaagaaag cacaggtgaa
    33781 ggtggcgaac cagaagtgat cggttcaaca gaagaataat actgaaacaa tcaggtggtg
    33841 ttagaagcgg cgggtcagca gcttttaaca cgcctttttt atttctaatc gtttattttc
    33901 atatggggct tccacttttg taaatggctt tacaaacgaa aaatcctaat ttaccaagct
    33961 tttcctcgca tacttttatc gtacctttat aaaaatttat actttgaaaa aaagtattca
    34021 attattgttg catttttcct gattctttat tagaatgaac cctcgttgaa gtaagtattt
    34081 ctagtttatc ctaggattaa ttataaaaat aaaacattcg ttgattcgaa ggaggttaaa
    34141 cagatgtttg aaagttttga tagcagccgc aaccgttacg cttcagttgg tgtcgtatca
    34201 agtttgccag atgaattgat tgacagtata tggtttatta ttgacctaga tcttaaggga
    34261 gtcattccat tagaaaatat attaaccttt gatttgatcg aaaatcgtgg aaaagtgacg
    34321 atgcattttt ctcaagcagg aagcaatgtg gaaatggata ttgatttgcc gtttgcctat
    34381 tcgaaccaat ttcctgctca agtatacgcc tatgatgacg gtacgagaga aacgatcctt
    34441 ttaccaagtg agattagaca atattaaggt gatagataaa cgtggatgtc ccttatttta
    34501 cagaccaggc caagttgttg attctttgac tcaattcttg gcttcttttt atggagtttc
    34561 ataaattatc attgatttct aattggtatt ctcaggataa acgctatata ctactaacta
    34621 aattttaaag atataaggag tttaaaagat gcgcgattta acaaaaggaa gccccgctaa
    34681 actgattttg atgttcactg ttcctttact gatcggaaat gtttttcagc agttctacaa
    34741 tatgatcgat atgatcatcg tgggtcagac gctaggtaaa aatgcgctgg cagcggttgg
    34801 tgcaactgga agcctgacat ttttgatcat tggctttgcc caaggtttaa cagctggttt
    34861 agccattatt accgcacaac gttacggtgc aaaagattat cggggtctga agaaaagttt
    34921 tgctgctagt gttgtcatta gtcttgtcgt cactgtgatt ttaactgtcc taagtctcct
    34981 gtttattcgt ccgatgcttc aattgatgca aactcctcct gaaatacttg atcaagcaca
    35041 aacatttatt tcaattattt tattagggat ctttgcatca gtcagtttca atttgctttc
    35101 taatgtcatt cgcgcacttg gtgacagccg cacaccacta ttctttctga tcattgcggt
    35161 catcatcaac gtcgtacttg atttgatctt tattatctat tttggcatgg gtgtggaagg
    35221 tgcagctatt gctacagtaa tcgcacaagt ttcttccagc gtactgtgtc tagtgtatat
    35281 caagaaaaaa atgcctttgt tacagttacg caaaaaagat ttcaaatttg aaaaagaaga
    35341 gtttgctgta cacctaaatg ctgctttgcc aatggcgttt caatcttcga tcatcgctgt
    35401 tggtgctatc gtgttacaag cagcactaaa tagtctaggg acagatgtcg tagctgctca
    35461 agccgctgct ggtcgtatcg accaatttgc aaatcagccg atgatgtcat ttggtattgc
    35521 aatggcaact ttcactgcac aaaactatgg tgcgaaagaa tatggccgaa tcttgcaagg
    35581 ggtacgtcaa actttactga tgagtgtcgg atttagtctc ttagcaggtg ctatcgtcat
    35641 tttcttcggt catttctttg ttcaattgtt tgttagccca tcagaaacac gtgtatttga
    35701 attagcacaa acctacttca atatcaatgg gtctctttac tggatcttag cagtcctgtt
    35761 cattttacgc tacacacttc aagggcttgg acaaagcaag atccctacga tcgccggtat
    35821 gatggaattg cttatgcgtt cttttgcagc tatcgttctt acaggaatgt ggggataccc
    35881 aggagcagca gcagcttctc ctttagcttg gatcggttct gttgccgtgt tgctttactc
    35941 ttatatgcgt tcgatgaaac aattgaaaca catgggtgaa gaggaacatt tcacattaga
    36001 acatgcagaa gcaagatagc cttgattaaa agaaatcgtt gcggctaaaa atgaaaactt
    36061 ttattgaaaa tatcttcttc gtttaccgaa aacaggtttt ttagataaca aaaaggctgt
    36121 gacaaaaaat gtcacagcct tttttctttt attctatttt tctttccgtg gactctactt
    36181 tgaaataatc gatatgtgca tgctggtatt ctaataccgt gaaacggaat gatgcaatct
    36241 caatgattgt tcctgctttt atttctttat atcggctaga agcaaagccc gcaagtgtag
    36301 taaaattcgt ttcttcaaac tctggaatct ctacatgaaa ataacgctgg aaatcatcta
    36361 atgttagttt gccggagatg atataagagc catctgactg ttccgctatc aatgcttcgt
    36421 ccaaatctgt ctcatctcgg atttccccga cgatttcttc taaaatatct tcaatcgtca
    36481 ctaagccgct tgtccctccg tattcatctt ttaagatagc aatatgcttt cccttttttc
    36541 gcatgatcgt caataatttt tttataggga tcgtttcagt tgttacgatc ggctcttcaa
    36601 tcaactgttc tactgtcagc tggtcatctt tataagattg cttgatcaaa tctggcaagg
    36661 tgacataacc taaaacattg tcttttgatt ctaagataac cggatagcga gtatgtcctt
    36721 gttcaatcga agcttttatc gcttctttga cggtttctga agcttcgaat acttgcatgg
    36781 acgtacggtc tacttgtata tcttttgcca aggtatcatc aaattcaaac acgttttcca
    36841 aataatgata ttcttctttg gtcaactctc ctttttcata agaattcaat gcgactaatg
    36901 tcagctcttc ttgagacaat gtctcttctc cttcagatac gagacgcaca cctaatagct
    36961 tccctagccc tgcagctgaa tgattcaata cccaaataaa aggaaacatt actttataaa
    37021 aataatgaag tggtttaact acgaacaaga cgaccttctc cgtattgacg atactatagc
    37081 tcttaggaag cagttcgcca ataaccacat cgatatacgt aactaaaata aatcccagcg
    37141 ccacagagat tgtactgcta atagccgaac taaaaggtag tttaccgatc actggttcta
    37201 gtaaagccgc gacagatcct tcaccgaccc acccgatgat caaaccggtt agcgtaattc
    37261 ctaattgact agctgataaa tagttatcta aatggtgtgt aacatgaaga gctagcttgg
    37321 cgttctttac attttccttt tctagttgtt ccaatcttga tcgtctgatt ttcactaaag
    37381 caaattcact tgctacaaaa aatgaagtaa ttgcaatcat caaaaataaa ataatgaaat
    37441 tgataaccat attctcccgc ctttctcttt ttctattgta ccctttttct acaaaaagat
    37501 tgaaaataat gcgtattcat atttaggaat aaaaaaggag atgcggcatt tttcagccac
    37561 atctccttat cacttcaaaa tcattgcgtt acttgttgct aaacagcttt ttcatgacct
    37621 ctgataaacg gtgttcaagt agctgacatt cgacattagt tcaggtcaaa gcgctacttt
    37681 cacaaccgct ctttcaacca tgttttccga gtatttccaa tgataagcgg aacagtttca
    37741 tgttttactt cgctgtactc tagcccaaac catgtttctg gcgaggtagc gatttttttg
    37801 attgccagtt ttgcctgcat gatttgacgt tgccattttc ctagagttgt cacattggaa
    37861 actacttcat caatcaggac aaattggaaa tcgccaactt ctcgccctgg tgtcagagag
    37921 tatctttgtg gctgttttgg caaacggcct tgtttcatca aatcatggat gacttggcga
    37981 acatatacgt tgatttcctg ttggaccctg aaacctaaga acagattcac ttggacaata
    38041 aaatcagtgt ccatcatatc tacttgatat tctttcgtat atggctcatc cgtcacttcc
    38101 acatgaacaa accaatagac tcgtgctctt ttaggccgtt tatctaaaat cgaatagatg
    38161 aactgtcggc caactttatc ttcagccata tcaggaacca agaagaccac atttgtctga
    38221 tagattggta aagaagtatc atcacgtaat tctctaagct gcgggatata atctctcaaa
    38281 gacacatcct cggttgacga ttcgcgaata atgttccctt gttcccaaat aaacatcacg
    38341 actaagatga tcaaaccaat cagaacggct acaaaaccgc ctcggaagaa tttgacaaca
    38401 cttgagataa agaaaacgac ttctatcgca ccgaagaaaa aggtaactaa atatgcaagc
    38461 caacgagaat gcccattttg gataagataa aagtaaagca gtgctgttgt catcaacatc
    38521 gtcacagtga tcgatagacc gtaagcagct tccatatgat gagacgttcg gaatgtgaca
    38581 accactagag aacaggcgat ccataaaatc agattgactg ctgggatata catttgcccg
    38641 atactgttcc caggatacat gatctttaac cgaggcagca gtttcaactt gattgcttct
    38701 gaaactagcg taaacgaacc tgtaatcaat gcttgcgaag caatgactgc agcgactgtt
    38761 gcaaaagcaa cgccgatcac gatccaactt tgaggcatca tttggaagaa tggattcagg
    38821 ttcttgattt gctgtgcttc agggttttga taaacattca acaaccacgc agcctgccca
    38881 aaataattga taaccaaaca aattttgacg taaggccagc tcagacgaat atttcgtttg
    38941 ccgacatgtc caagatcgga ataaagcgcc tctgcacctg tcgtagccag gaaaatattt
    39001 ccaagaatga acaagcctaa tttgttatct gcactaaaaa gcagctggat agcataataa
    39061 ggattcagtg cacggataac tgtccagtct tgactgaagt tcattaatcc cattacgcct
    39121 aaaaaagtaa accaagcgaa catgattggt ccgaatgctt ttccgacaat atctgtacca
    39181 aagcgttgga ccatgaataa aacaaaaata ataactaaag tgataatgac gatcacgttc
    39241 tggttattac cgaaccggtc aaaaaactgc ggaattcctc gcaagccttc aatcgcagtc
    39301 gttaccgtca cagcaggggt caatacccca tctgctagta aggcagctcc tccgatcatt
    39361 gcaggaatga tcaaatattt cccacctttt cggacaagtg tgtagagtga gaaaattcca
    39421 ccttcaccat ggttatctgc attcaatgca atcacgacat atttgattgt tgtcagtaga
    39481 gtcaatgtcc aaaaaatcaa tgagactgat ccgataacaa aatcaggatt gattcctgac
    39541 aatccgccat tatcttcaac aatcgatttc ataacgtaaa gcggacttgt cccgatgtct
    39601 ccgtagacga ctcccatcgc tacaagcagg ccagccattg atatcttttt ggtattgtgc
    39661 gttgttgcca cgtttttccc tcatttcttg ttatatggat atcctttagg taagcttgaa
    39721 tgatagatga tattcttatc catgacaccc gctttcctaa attgcttcta attatccaag
    39781 taacacactg aagtgtcaac ttatttgaat aaaattattc tttctttgga cgttctctga
    39841 tacgtcttgc cacaaatgaa taaagagcaa ggcaaaaaat tgcacccaat aatgctggta
    39901 tcaaataaaa accgccgagt tccggtccca gtggtcctag taaaaattcg ccgagccaac
    39961 tacctaaaaa accgataatg atatttccag atacgcctcc cgggacttcc tttcctaaaa
    40021 ttgcgccagc tatcaaacct aatataccgc cgataaataa cgaccataaa taattcagca
    40081 tctcctttcc ctcctttatc catatttctc acttttattt tacgctttat tcagaaggac
    40141 tccaaaaata tgcgccaaga aactccagaa aaaaagaagt tgtaacaaaa gtgttcagct
    40201 tatttccgta gaagctgctt ctggaacacc gtgaatcaga ggttgtgaaa tcagcggttt
    40261 gacctgagta ttagagaaac aaacggaaaa atagttcctc atattttgcc gttttttgag
    40321 ctaatatcga gggacagctg attgaacatc gtttattgga gaggttgtaa aaaagctgtt
    40381 ctgcatcaag taataagaac agccaagtaa aaacagctcc taatgttttt aacttggctg
    40441 gacttattac cgcagatgca agcttttgaa caccgtttat tcgaaaaaaa aggactgtga
    40501 cacggctttt gtcacagtcc ccttttttcg aataattatt gtccccctgt caccttcacg
    40561 atcacaagta cattattcct atgaattaaa ttatttttct tcatttttcg ttgaaccatc
    40621 ttcgttcaac attgcttttc ttgacagatt tacgcgtcct tgtttatcga tttcagttac
    40681 tttcacaagg atctcatcac ctagtttaac aacatcttcg actttattga cacgatcgtt
    40741 tgctaattgg gaaatatgga caagtccatc ttttcctttg atcaagttga cgaatgcacc
    40801 gaatttttcg atacggacaa ctttccctag ataaacttca ccaactttga cttctttggt
    40861 caaatcttcg atgatcttga tagctttttt gatcatttct gcatcagctg aagcaatact
    40921 tacttttcca tcttgatcga tatcgatttt cacaccagtt tcatcgatga ttccgttgat
    40981 cgtatcgccg cctttaccga taacatcttt gattttagca ggttcgatct ggatcatttc
    41041 gatttttggt gcatattggc ttaattcttc acgtggagca gcaatcgttg aagtcaattc
    41101 ttctaagatt tccatacgtg cttgttttgc ttgagttaat gcttcagtta ggatttgttc
    41161 tgtgattcct tgaatcttga tatccatctg taaagcagtg ataccatctt ttgtaccggc
    41221 aactttaaag tccatatcgc caagatgatc ttccagccct tggatatcag ttaggatcgt
    41281 atagttttca ccatcagaaa caagtcccat cgcaataccg gcgactggcg ctttgattgg
    41341 tacacctgca tccatcaatg ctaatgtgcc agcacagata cttgcttggg aagaagaacc
    41401 atttgattcc aatacttctg ctactaaacg gatcgtataa ggaaattctt cttctgaagg
    41461 gattacttgt gctaacgcac gttcacctaa tgcgccatgt ccgatttccc gacgccctgg
    41521 agagcctgca cgtcctgtag aaccaacaga aaattgaggg aaattgtagt ggtggatgaa
    41581 tcgtttgctg acttccaccc ctaaaccatc aatgatttgg tgctctccta gaggtgcgag
    41641 tgtacaagct gataaagctt gtgtttgtcc tcgtgtgaac aaacctgatc catgtacacg
    41701 tggcaataag ctgacctctg atgacaatgg acggatttca tccaatttac gcccgtcagg
    41761 acggatctta tcgatggtga tcaattcacg tacgacatct ttttcaagat cttctgtgat
    41821 ttgtttaact tctttcaaca agtgatcttc atcttcatga cctgcaaatt tctcagcata
    41881 agcttcgcgg atatcgatct tcacctgctc gatattatct tcgcgagcaa gtttttcttc
    41941 agtcattact gctgctttca ttgtagcgta agaagcatca aagatttctt ttttcaatac
    42001 ttcatcaact tgcagtaaag tcacttccat tttttctttc cctactgctt gaacgatttc
    42061 ttcttggaaa gcaactaact ctttgatagc gtcaaaacca aacagcaatg caccaagcat
    42121 gtcttcttct gaaacttctt tcgcgccgct ttcaaccata ttgatggctt gtttcgttcc
    42181 ggcaacagtc aattcgatat ctgtttgttc tgcttgttct actgttggat tcaagacgta
    42241 ctcgccattt acacgaccaa cttctacgcc tgcgattggg ccgtcaaatg ggatatctga
    42301 gattgatagt gctaaagaag aacctagcat tgctgccata gctggcgagc agtctgtttc
    42361 tacactcatc acgatgttcg ttacttggac ttcattacgg aagccttctg caaacatcgg
    42421 gcgaattgga cggtcgatca aacgggcagt caatgttgct tccgtacttg gacggccttc
    42481 tcgtttgatg aaacctccag ggattttccc tgctgcatac attttttctt cataattgat
    42541 cgttaatggg aaaaagtctg tatcttttgc ttctttagag gccacagctg cacttaagac
    42601 tactgtatct ccataacgga ctaaaacggc tccgtttgct tgttttgcta gctgaccgac
    42661 ttcgatttgt aagggacggc cgccccatgt cgttttgaat acttgttttt ctgacataac
    42721 ttctcctttt ccttctcgtg aaagcaagac gctactaaac aaatgaaaac tgccgaaagc
    42781 cgtcttttga gcagctttca tttggttagt aggagtccat ctagcttgat cacggaattt
    42841 tacttaattg atatacgagt aaaaaaagtg agattcatgt gaatcccact ctttttgact
    42901 aaaattaacg acgcaagcct aaacgtttga tcaattcgcg gtaacgttga acgtcagttt
    42961 tacgtaagta agctaacaag ttacggcggt gaccaacttt tttcattagg ccgcggtatg
    43021 aatgatggtc ttttttgtgc acgcgtgcat gttcattcaa gtggttgatt tcttcagtta
    43081 atacagcgat ttgtacttct ggagaaccag tatccccttc atggcgagcg tattcgttga
    43141 tgatttcatt cttgcgttct tttgaaattg ccatttcttt cacctctttc gattattccc
    43201 ctgcgactga gtaaaacgtt ggtgattcgc ttaaccaagt ggcaggtcat gtgcctagag
    43261 cacacagcta tactttacct gaaaccgggt tgaaaatcaa gtagttaatc attaagaaaa
    43321 ggtagttttc aaacaatctt actactactt ttccattttt gtggtaaact ataatgcgaa
    43381 aatagacttg aaacacagtc ttttaagaaa ggggtacata ttccatgatt acaatggatg
    43441 atatcattcg tgaaggaaat ccgaccttac gcgaagttgc aaaagaagtt tctcttccat
    43501 taagcgaaga agatatctct ttaggaaaag agatgcttga gttcttaaaa aacagccaag
    43561 atccaatcaa agctgaagaa ctacacttac gaggcggtgt tggcttagct gctcctcaat
    43621 tagatatttc taaaagaatc attgctgttc acgtaccaag tcctgaccca gaggcagatg
    43681 gaccttctct aagtactgtt atgtataatc ctaaaatttt gagccattct gttcaagatg
    43741 cttgtttagg ggaaggtgaa ggctgtttat ctgttgatcg agaagtacca ggatatgttg
    43801 ttcgccatgc caaaatcact gtgtcttatt atgatatgaa tggtgaaaaa cacaagattc
    43861 gtttgaagaa ttatgaatcg atcgtcgttc aacatgaaat cgaccatatc aacggtgtga
    43921 tgttctatga ccacatcaac gatcaaaatc catttgcact aaaagaaggc gtattggtga
    43981 tcgagtagag aattggcagg ttccttcgat actatgctac aagaaagctt tataaaaaag
    44041 gggagtgctc aaaagctgca cttccctttt ttatgtttct ccactaatcg aaatttagat
    44101 agacatctag aaatcgcaat tccaaaaaat tctgtgtatt ggatacgcat cttttcttat
    44161 tttttcttat ttcttccata tgggtgttta tctcagagat gatagaatag tttctaggat
    44221 aaaaagtagc agctacgatg ttatccttcg tagaggattc ctgcttcagc tagtttttgt
    44281 ttgatgtttt tcagcatatg cttttctggg tagcggatcg tatgcagatg gatgccgcct
    44341 gttaattcgg ataacaaagt agcattactt ttttgataac gtctcataaa ggaatcgata
    44401 tcttgtggcg ttttgatgtg caaagttcct gctaactcac cgtaaagggg atgttccaca
    44461 attacatcaa tgatttcacc accattttca acgattagtc tcagttcttc ttctgtttga
    44521 tcttttccat gctggacagc gattttactg atgtgcccag tattggtttc ttctgtttcg
    44581 aggatataac ctcttgctgt tgccacgata cttttacctg cagctcgtag taaagcaata
    44641 tcgcctacaa tgatctgacg gctgactcca aaagttttcg caaacttgct agcactcact
    44701 ggtttttctg tatttgccaa ggtttgcatg atttgttttc gccgtgtctc tccttcgatc
    44761 acacttattc ctccttcatt ttgttttggt attcgcgcga gtgtttcatc aaatcaacat
    44821 acgtgtctgt tattgtttcc ttatatccct tatttttcag cagactaaca actttatcca
    44881 atacttgctg ctctcttttt gcatcataaa ctgcttggtt ttcttctttt ttcacttgtc
    44941 ctattttagt taccgtatcc attctttttt ctaataaagc aaccaactct cgatcgatca
    45001 tatcgatctt atgacgtaat gtttcaagag actcttctgt tagttcttcc tctactgtct
    45061 tgatgatacg agcaggattt cctgcaatca ctacattgtc gggaaaagat ttggtcacaa
    45121 ccgaacctgc gccgactact acgttgtccc ctagagtgac acccggcgtg acgataactc
    45181 ctccgcctaa ccaaacatta ttaccgattt taatgggttt tgcgtactcc agaccgctgt
    45241 tacgtttcac tggatgtagt ggatgcgtcg ccgtgtaaag ctggacattc ggagcgaaca
    45301 tgcagttatc accgatttca atcgtgctga catctaaaaa tgtacaattg aaatttgcat
    45361 aaaaattctc cccaacaaat atattatatc cgtaatcaaa attgataact ggctccatat
    45421 agattttttt ccctgtacgg ccaaaagtct ctttcaacaa ttgggatcgc agttccgctg
    45481 attctgcttg gttgatgatt tggctttgac ttcttgcaaa catacggtcg gcaaccagct
    45541 caggatcatt ggcaaaataa agctcgcctg caatcatttt ttctttttcg gttttgaatt
    45601 cagtcataat ctgctcctaa ttcttgtatt tttttgaaaa caaccgctcc gtcttcttga
    45661 ataaacgtat aaggctccgc taaataaaca ggcgtttgat tgattactaa gattttggct
    45721 gacggctggc gaaactgtac cagatcacaa aacggatgaa cttgaaaact cgttccaaca
    45781 atgacgatca gctctgcttg gctgacggca taagccgcct gctccatgac tccatcttga
    45841 aacccttcac catataacac gatgtctggg cgtatttgtc ctccgcatgc ttgatgtttg
    45901 tcgctgtgaa gatattcttt ccagtctacc gtttgttgac attttcgaca atgacattga
    45961 tacagactcc catgaaaatt gacgagttgc gggcttttag cctttgcgtg caaaccgtcg
    46021 atgttttggg aaacaaccca aacatttttt tctttcgcta gatgtgctat ttccaaatga
    46081 atgatattgg gttttgcttc aggatgataa atctttttga caaattgata gaatttatcc
    46141 ggttcattca ccattgcttg atgactcagc aagtattccg gctgctccat cccgtggtag
    46201 attcctgaca aagatcgata gtctggcact ccagaaggtg tggatacccc tgcaccggtc
    46261 aaaaatgtaa tttgctgata agctaatatc tcttttgctg cttcgtctac tgttaattgt
    46321 ttcatgtttt gtcacctcac tttccattat agcccatact cgaacaaatt tattgttgaa
    46381 aagtcagaaa aatgggaata aaaaagtgtt atgggcattt gagcaattta tcacaatgtt
    46441 cactgacaaa agaatgaaaa cgtgttacaa tactgctgta taaaagtgag gtgaatcgat
    46501 atgttaccat tctttattta ttggggcgtt attctggctt gtatcgcctg gttagcgtta
    46561 agcgtttatt tctctgtttt ttatcttgta cgcaaagaaa atggaaatct ttgggctttt
    46621 gctttattca acgtgattgc cgcaattgtt ttagccatta cattagccgt atatcgaaca
    46681 tggggctggg gaatcaccca atactcaagt ttgatttatc taatattagc catttatggc
    46741 gtcgttgttc ttttgcaagc gatcctagga cgtgagccaa agaaagcggc agcttaaatg
    46801 atcgagatgg aactggacac aggaaatttt ctgttctagt tcttttttta ttgtattaca
    46861 tacatttttg tgtataatta tactatcgtt tgcaatggat agatgattca cattttgatt
    46921 gttttttcgg taaaagtaaa gatttctata tttatcagta ttttttcaaa aaccagcagt
    46981 tatagattga caaagcctct cgagtatcgt aaactttttt tgtataaata actgaatacg
    47041 agatgcctca atcaatgagg tagaggtcgc gagaatcatt aaccctgtgg agtgaagcaa
    47101 acatggagaa gcaggaatag ggaggatcgc cgaaatgtgc acttttttgc ttggagtgta
    47161 tgttgggacg caagttaaga gcttgcggac tgtcttagca gtgatgctaa gttgcgctat
    47221 gttttgtgag aggtttatca ccttatggaa ttttagcggc ctttttatat ctataaatcg
    47281 gtcgcttttt attttttcag ataaagcttt accgcatttc atctagaccg ctcgtgtatc
    47341 atataatcag aaagggagaa aaaaatgaaa aaaaatatgg tttcagttgc cgtactattt
    47401 gcttttgtct tctcactatt cagtgtggga tctctggcaa atgcagatga aaaaacaccg
    47461 agcgatgagt ttcgcgtagg tatggaggct gggtatgcac ccttcaactg gtctcaacaa
    47521 acagatgaga ataatgcctt gccaattcaa ggccaaaata gttacgccgg cggttatgat
    47581 gttcaaatcg ctaaaaaagt agcagatggt ttagggaaaa aattagtcat cgtccaaacg
    47641 aaatgggatg gtctggctcc tgcccttcaa tcaggcaaga tcgacgcgat catcgccgga
    47701 atgagcccta ctgctgaacg acgaaaagaa attgatttta ccgatcctta ttacgaatca
    47761 caattagtag tagtagttca aaaagatagc aaatatgcaa aagcaaaaag tttagcagat
    47821 ctttctggag caaagatcac tgctcagtta aatacattcc actatactgt gatcgatcag
    47881 ataccagatg tgcagaaaca acaagcaatg gataatttct cagcaatgag aacagcttta
    47941 gcttctggaa tgatcgatgg ttatgtcagc gaacgtccgg aaggtgtaac ggcaactagt
    48001 gtgaacaaag acttggaaat gcttgaattc agcggtgcag atggcttcca gacaaacccg
    48061 gaagatgttc agatttctgt tggtatgcgt aaaggcgacc cggatatcca aaaagtaaac
    48121 cagattcttt ctggcatctc taacgatgaa cgaaccaaaa ttatggacca agcagtcaaa
    48181 gatcagcctg ctgcaactga tagtgaagaa aaacctggca tcatttctga tttcactacg
    48241 atttggaaac aatacggcaa tatgttctta cgaggtgctg gacttacctt gttcatcgca
    48301 ttgatcggga cagttgtcgg tactacactt ggattactga ttggtgtctt ccgttcggta
    48361 cctgaatcag gaaatgcact gactcgcttc ttccaaaaaa tcggaaatgc acttctttct
    48421 atctatgtcg aagtattccg cggaacaccg atgatggtac aagcaatggt catcttctac
    48481 ggtctggcac tcgcttttgg catttcattg aaccgaacga ttgccgcttt gtttattgtc
    48541 tctctcaata ctggagctta catgacagag atcgttcgtg gggggatttt tgcagtagat
    48601 gaagggcagt tcgaagctgc gcaagccatt ggtatgaccc atggccaaac gatgagaaaa
    48661 gttgttattc cacaagtttt acggaatatc ttgccagcaa caggaaatga gtttgttatc
    48721 aatatcaaag atacagcagt tttaagtgta atcggcgtag ctgatttgtt cttccaagga
    48781 aactctgctt ccggtgcgaa tttccaattc ttccaaacat ttacgatcgt cgggatcatc
    48841 tacttagtca tgacctttac gatcaccaga atcctgcggg cggtagaaaa gaaaatcgat
    48901 ggtccttcag cttataccaa gcttgaagaa atcgacaatt caaatttgca ggaataagga
    48961 ggaagaatca tgaatgaaaa aattattgaa atcaaacatc taagaaaaag cttcggtgaa
    49021 aatgaagtat tgaaagatat cagcgtaaca gtcaataaag gtgaagttgt gacgatcatc
    49081 ggttcttccg gttctggtaa atctactttc ttgcgctgta tcaatttact ggaaaaacca
    49141 acaggcggcg agatcattta taatggagaa aatgtattag ctcctaaata taatttacct
    49201 aaatatcgaa cgaatctagg tatggttttc caatccttca acctgttcaa caatatgaat
    49261 gtcttggaaa actgtatgtc tggtcaaaca actgtcctga aacgagataa agaaactgcc
    49321 aaaaaagtag caatcgaaaa tctggaaaaa gttggtatgg atcgttatat cgaagccaaa
    49381 ccagcacaat tatctggtgg acagaaacag cgtgtcgcaa ttgctcgggc attatcaatg
    49441 aatccagatg tattgctatt cgacgaacct acttcggcgt tagaccctga aatggttggc
    49501 gaagtgctta atacgatcaa agacttagca catactggct tgacaatgct tatcgtgacg
    49561 catgagatgg aatttgcaaa agatgtatct gaccgtgtca tttttatgga taaaggtgtg
    49621 attgcagaag aaggtacacc agaagatatc tttgtccatc cgaaagaaga acgtacaaaa
    49681 gaattccttt cacgtatttt aaatgcttag gatcaatagc tgattgggac aaagagctgc
    49741 ggtgtttacc gcagttcttt tttatgaatt actcataaaa ggtaaatttc tgaaaatttt
    49801 ggaatcttag ttgtgttttt cagaaaactc tcttaatatg gaagtaatta aactttttta
    49861 gttaggatga tttaaaaatg aatgaagagc aagaactgca gcgtggactg aaaaatagac
    49921 atgtccaact gatttctatt ggcggagcga ttggaacggg gttgttttta ggttccggaa
    49981 agtctatcca gctagctggc ccttcgatat tgctcgccta tcttattact ggatgcatct
    50041 gttttttcat tatgcgtgcg ttaggcgaac ttctgttatc aaacaccaat aaccattctt
    50101 ttctggattt tgttgcagaa tatcttggca aaaaagcggc tttcatcact ggctggacgt
    50161 attggttctg ctgggtatcg attgcaatgg ccgatttaac agccatcgga atgtatgtac
    50221 gctactgggc gcctggtgtt cctcagtggc ttcctgaact gattgcatta ggtcttcttc
    50281 tttgcttgaa tatggtggct gtctcattgt ttggtgagtt agagttttgg tttgcattga
    50341 tcaaagttgt agcgatcatt gcctttatcc tcgtaggagc ttatatgatc ctcacccact
    50401 atacgacaga tattggcaca gccagtatca cgaatctatg gtctcatggc ggattctttc
    50461 caactggtgc aaaaggattt atcttagctt tccaaatggt aacctttgct tttgctggga
    50521 tagaattagt tggattagtc gctggagaaa cagaaaatcc tgaaaaagtg ttgccggaag
    50581 ctatcaataa tattcctatc aggatcatct tattttactt aggatctctt tttgtcatta
    50641 tggcaatcta tccatggaac agcttgaatc cagataacag tccatttgta gaagtatttt
    50701 cggaaattgg gatcacgatt gctgcttcct tgatcaattt agtcgtatta agtgcagcag
    50761 cttctgcctg caacagtgca atatatagta caggacgtat gcttcgttct ttagcgcaag
    50821 aaggcagtgc ccctaaaaaa ttcaaaaagt taacaacaca tcatgtgcca gggaatgctt
    50881 tgacattttc tactatcgtc attttcattt ctgttatctt gaattacgtg atgcctagtg
    50941 aagtattcac actagtaagc agtattgcca ctacttgttt cttgtttatc tggagtatgc
    51001 ttgtctacac gcacatgaaa tatcgtaaaa gtatcctcgg caaaaaagct cattcattta
    51061 aaatgccgtt gtacccattt agtaattatc tggtatttct ttacatggcc tttgtgtgtg
    51121 tcgtcttgtt cctaggaaaa gatacgagaa tcgctttatt actaacacct gtgtggttct
    51181 tgcttctatt actaatctat catatgaaat atgaacgtaa gaagtaagaa agtagttgtg
    51241 acagaagtgg tcagcttcga gaaataagaa ggaatttacg aaaattgctt cttaaatttt
    51301 tgtgaaattt cggcttattt cttggagata aaagcttctg tcccagcctc ttttctattt
    51361 ccccggaaac aaccgctgat aataggcatc tgctttttta taggaaaaaa tccaaatacc
    51421 agatacaaaa aaacgtccga ataacccgat caacggtaca aaaaagacac cggcaatcaa
    51481 gcataagtta atcatcatgg agataagact tcgacggtcc tttgacaaaa ctctcacgac
    51541 atgggaatct ggtttatttg ctttcatgat ttgcctcatc aagataccaa aagaaaaact
    51601 ccacatcgtg tatactaaga cataagccat ctcaggccaa aaagcttcag ggttacgccc
    51661 gacccaacta gtaacagaag gacataaagt cagccaaaag agcaaaaaga tattcgacca
    51721 gaccactcgg taattaattc gttctgccat ctgaaatata cgatgatggt tcacccagat
    51781 caccgcaaca aatataaagc tgatggtata agctaataat gtatttcgga tgacccataa
    51841 agaatgaaag ctagatgtat tagggatctt gatctctaat acaataactc tcaaaacaat
    51901 ggctaccact ccatcagtaa atgcttctaa tctcgacttt gtcacgatga ttcctcctca
    51961 gatgattcag cgcttttatt ttatcactta cgttattaaa taaccatcgt tttatatagg
    52021 gaattttctg aaaattgttg catatttttc gaaattttgt tacttttaaa aaaaccgtaa
    52081 aggagttatg tatgacaaaa aaattatcat ttaaagacta tctttttata ggctccatgc
    52141 ttttcggtct attttttggt gctggaaatc ttatctttcc cgttcatttg ggtcaagaag
    52201 caggcgctgc taccttttgg gcgaatctag gtttcttggt taccggtatc ggtcttccct
    52261 tcttaggggt gattgccatt ggtgtatcga aaagctcagg tgtatatgaa ttagcaaaac
    52321 ggataaatca aacatatgcg atggttttta ccatcttgct ttacttagtt atcggaccgt
    52381 tttttgcttt acctcgcctt gcaaccacat ctttcgagat tggtttagca ccatttatcg
    52441 atcctagcag acagacgcta tatctagcaa ttttcagtat tgtatttttt gtgctggctt
    52501 ggtggttttc aagaaaaccg acaaagatat tagattatgt tggaaagttc ctgaatcctg
    52561 catttttggt tttattaggt gctttgcttc tccttgcttt tttgaatcca ctaggttctg
    52621 taaatcaagc gcctattcag ccaaattatc aagagcatgc ttttttcaca gggtttactc
    52681 aaggatataa cacattagat gcgcttgctg ctttagcatt tgggatcatc attgtcacaa
    52741 caatccaaaa catgggtgta acaaaacctg ctgaaatcgc taaagacacg atcaaatctg
    52801 gtgcgatcag cattatattg atgggaatca tttatacttt gctcgcttat ttaggtgcta
    52861 tgagcttagg tagctttgct ctaagtgaaa atggcgggat cacgctggct cagattgctg
    52921 atcattattt aggtacttat ggcagtatct tattagcatt tatcgttata ttagcttgct
    52981 tgaagaccgg tattggtttg attacagctt ttgcagaaac attcacagat ttgtttccgc
    53041 aaagaaatta cgcattcttt gttgcacttg ctagtatcct tccttgttta gcggcaaacg
    53101 taggcttgac gaatatcatt caattttcgt tgcctgtttt gatgttcatc tatccattag
    53161 caatgacact gattttacta gtgttagttg gtcctttgtt taagcagcgt acggctgttt
    53221 atcgaatgac tacttatttt acattgattg cttcgatttt tgatgggttg aacgcttgcc
    53281 cagaaactat caaacaaaca cctattgttc aaaatctttt atatgttgcg gaaagctact
    53341 tgccgttttt caaattgggg atggggtgga tcgtaccagc agttatcggt tttgttatcg
    53401 gactgatttg gagtttcgct aagaaagaag aagtagcaga ttgataaaag aggttgtgaa
    53461 agagacgttc agccttgagc aataaaaagt aattttgaaa aatggcttct cacatttttg
    53521 cataaatttg acttaatacc gagaaactga cttttgaaca ccgtttattg agaggtcgtg
    53581 aaaaagccgc ttagtttcaa gtattaagaa atattttgtg aaaatggctt ctcacatttt
    53641 tgcataaatt tgacttaata ccgagaaact ggcttttgaa caccgtttat tgagcgtcta
    53701 taaactgacc tgagtattag agaaacaaaa cggcaaagta gctccatata ctttgccgtt
    53761 ttgttttgaa ctaataccga agagaagct
//
LOCUS       DAGCYL010000002        50297 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.002, whole genome shotgun sequence.
ACCESSION   DAGCYL010000002 DAGCYL010000000
VERSION     DAGCYL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.002"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..314
                     /locus_tag="MB216_000048"
     CDS             <1..314
                     /locus_tag="MB216_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBT4524915.1"
                     /translation="IFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLK
                     KPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILT
                     I"
     gene            595..1815
                     /locus_tag="MB216_000049"
     CDS             595..1815
                     /locus_tag="MB216_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBT4524916.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1812..4532
                     /locus_tag="MB216_000050"
     CDS             1812..4532
                     /locus_tag="MB216_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4524917.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4516..5460
                     /locus_tag="MB216_000051"
     CDS             4516..5460
                     /locus_tag="MB216_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4524918.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5529..6539)
                     /locus_tag="MB216_000052"
     CDS             complement(5529..6539)
                     /locus_tag="MB216_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4524919.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6719..7063)
                     /locus_tag="MB216_000053"
     CDS             complement(6719..7063)
                     /locus_tag="MB216_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBT4524920.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7056..7490)
                     /locus_tag="MB216_000054"
     CDS             complement(7056..7490)
                     /locus_tag="MB216_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBT4524921.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7628..8365
                     /locus_tag="MB216_000055"
     CDS             7628..8365
                     /locus_tag="MB216_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4524922.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8358..9569
                     /locus_tag="MB216_000056"
     CDS             8358..9569
                     /locus_tag="MB216_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4524923.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9773..10555
                     /locus_tag="MB216_000057"
     CDS             9773..10555
                     /locus_tag="MB216_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBT4524924.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10625..11287
                     /gene="trmB"
                     /locus_tag="MB216_000058"
     CDS             10625..11287
                     /gene="trmB"
                     /locus_tag="MB216_000058"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBT4524925.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11385..13139)
                     /gene="ade"
                     /locus_tag="MB216_000059"
     CDS             complement(11385..13139)
                     /gene="ade"
                     /locus_tag="MB216_000059"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBT4524926.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13271..14200
                     /gene="tadA"
                     /locus_tag="MB216_000060"
     CDS             13271..14200
                     /gene="tadA"
                     /locus_tag="MB216_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBT4524927.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14157..15206
                     /locus_tag="MB216_000061"
     CDS             14157..15206
                     /locus_tag="MB216_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBT4524928.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15203..15481
                     /locus_tag="MB216_000062"
     CDS             15203..15481
                     /locus_tag="MB216_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBT4524929.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15456..15926
                     /locus_tag="MB216_000063"
     CDS             15456..15926
                     /locus_tag="MB216_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBT4524930.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15943..16245
                     /locus_tag="MB216_000064"
     CDS             15943..16245
                     /locus_tag="MB216_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4524931.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16262..16633
                     /locus_tag="MB216_000065"
     CDS             16262..16633
                     /locus_tag="MB216_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBT4524932.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16605..16964
                     /locus_tag="MB216_000066"
     CDS             16605..16964
                     /locus_tag="MB216_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4524933.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17136..18143
                     /locus_tag="MB216_000067"
     CDS             17136..18143
                     /locus_tag="MB216_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4524934.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18168..19352
                     /locus_tag="MB216_000068"
     CDS             18168..19352
                     /locus_tag="MB216_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBT4524935.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19789..21372)
                     /locus_tag="MB216_000069"
     CDS             complement(19789..21372)
                     /locus_tag="MB216_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBT4524936.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPINHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(21950..22093)
                     /locus_tag="MB216_000070"
     CDS             complement(21950..22093)
                     /locus_tag="MB216_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBT4524937.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22316..22795)
                     /locus_tag="MB216_000071"
     CDS             complement(22316..22795)
                     /locus_tag="MB216_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBT4524938.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22868..23152)
                     /locus_tag="MB216_000072"
     CDS             complement(22868..23152)
                     /locus_tag="MB216_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4524939.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23230..23424)
                     /gene="ssrS"
                     /locus_tag="MB216_000073"
     ncRNA           complement(23230..23424)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="MB216_000073"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23496..24782)
                     /locus_tag="MB216_000074"
     CDS             complement(23496..24782)
                     /locus_tag="MB216_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBT4524940.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24920..25591
                     /locus_tag="MB216_000075"
     CDS             24920..25591
                     /locus_tag="MB216_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBT4524941.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25557..26045
                     /locus_tag="MB216_000076"
     CDS             25557..26045
                     /locus_tag="MB216_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBT4524942.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26058..27005
                     /gene="prmA"
                     /locus_tag="MB216_000077"
     CDS             26058..27005
                     /gene="prmA"
                     /locus_tag="MB216_000077"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBT4524943.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27006..27770
                     /locus_tag="MB216_000078"
     CDS             27006..27770
                     /locus_tag="MB216_000078"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBT4524944.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28003..30216
                     /locus_tag="MB216_000079"
     CDS             28003..30216
                     /locus_tag="MB216_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBT4524945.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30227..30673
                     /locus_tag="MB216_000080"
     CDS             30227..30673
                     /locus_tag="MB216_000080"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBT4524946.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30844..32103
                     /locus_tag="MB216_000081"
     CDS             30844..32103
                     /locus_tag="MB216_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBT4524947.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32192..33139)
                     /locus_tag="MB216_000082"
     CDS             complement(32192..33139)
                     /locus_tag="MB216_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBT4524948.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33533..34825
                     /locus_tag="MB216_000083"
     CDS             33533..34825
                     /locus_tag="MB216_000083"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBT4524949.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34840..36609
                     /gene="aspS"
                     /locus_tag="MB216_000084"
     CDS             34840..36609
                     /gene="aspS"
                     /locus_tag="MB216_000084"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBT4524950.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36718..37593
                     /locus_tag="MB216_000085"
     CDS             36718..37593
                     /locus_tag="MB216_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4524951.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37859..39742
                     /locus_tag="MB216_000086"
     CDS             37859..39742
                     /locus_tag="MB216_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBT4524952.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39817..40101)
                     /locus_tag="MB216_000087"
     CDS             complement(39817..40101)
                     /locus_tag="MB216_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBT4524953.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40091..40825)
                     /locus_tag="MB216_000088"
     CDS             complement(40091..40825)
                     /locus_tag="MB216_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBT4524954.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40954..41577
                     /locus_tag="MB216_000089"
     CDS             40954..41577
                     /locus_tag="MB216_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBT4524955.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41769..42890
                     /locus_tag="MB216_000090"
     CDS             41769..42890
                     /locus_tag="MB216_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBT4524956.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42897..46025
                     /locus_tag="MB216_000091"
     CDS             42897..46025
                     /locus_tag="MB216_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBT4524957.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46044..49238
                     /locus_tag="MB216_000092"
     CDS             46044..49238
                     /locus_tag="MB216_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBT4524958.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILPLYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49384..50091)
                     /locus_tag="MB216_000093"
     CDS             complement(49384..50091)
                     /locus_tag="MB216_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBT4524959.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16419 a         8847 c        10487 g        14544 t
ORIGIN      
        1 atatatttaa ccatttgaaa aagatgctaa atgtgtctgt tactgaatcg gcacctgtaa
       61 atattgaaat cgtacctcaa aatgtatcta aaggtgtggt gtttgatcgt ttggaatcaa
      121 tctacaattt aaagaaacct ttgagaatcg cgatcggaga tagtttaaat gacttatcta
      181 tgtttgaaag tgcagatatt agttttgcaa tgggaaatag tcatcaagaa atcatgaaaa
      241 aagctgatta tgtcactgtt tcaaatgata aagacggagt atctcaagca tttgaaagaa
      301 ttcttaccat ataaactaat taaggtagta tagaacatag aattctaaat acggaaaaat
      361 ggagcataaa ttccagtaca aatagtactt cttcagtgtc aaacgaagcg aatataacat
      421 agatatttag gattgtgaca aaaatcatgt cacaatcttt ttttcgaata aacttatttt
      481 ttgaagtcgg ccatttatcg taatttcttt gcataatacg aacaaatttt cggttattga
      541 tgaaaaaaaa cattgaaaat ggtatgataa aagaaaaaaa ggaggaaatt tttaatggtg
      601 aaatttatcc atgcggcaga tttacatatg gatcggtcat ttgaaggact gacaactctt
      661 gataaaactg tacaggaaaa actattgaag acgaatctga ccgttttatc aaatatcatt
      721 gaacaggcaa taataaataa cgttgatttt gttttacttg caggagataa ttttcatcaa
      781 aatcgtcctt cattaaaaat acagaaacat ttttcagaac agatgaaacg gttagaaaag
      841 aaccatatac ctgtatttat aatattcgga aatcatgatt tctatcaaaa agagcggtat
      901 tggttctcct ttcctaaaaa tgtccattta tttacatccg aaacggtaga gacaaaaaaa
      961 ataacgataa aaagtggaga aactgtctct ctcagtggat ttagttatcg tcaaccgtgg
     1021 attcaaaaag acaaagtgat ggaatttcct tctagagaac tcacagatta ccatattggg
     1081 ttgtaccacg gggaacctgg cgtctctcaa aaaggaaact atgctccttt ccaaccctca
     1141 aagatgcaag aaaagggata cgattattgg gctttagggc atatccatgt gccaacggtc
     1201 ttaaatgaaa agcaaacaat tgtttatcca ggtgcgcctc aaggtcatac taaaaaggaa
     1261 caagcttcta catctattct actagttgaa ttaagtaaag gaagttgcca aatacatccg
     1321 attaaagtag cagaggtcta ttggaagaca aaggaaatat cattgaggaa agcaagaacg
     1381 acaaaagagg tcattgttca cattcgtcag cagctttcaa aagtagagga tggatttagt
     1441 ttaattgaga taaaattaaa agattatgac catctcaata cagatgtttt ggaacggata
     1501 caatcaggag agctattaga ctatttgtta gaggaattct cagataggat aaatgatttc
     1561 tttatttggc gtatttctct catagagaat actttttata cgaagacccc acttgctgct
     1621 tctgctaaat tgatggaaca gttatttcaa tactatcaga cacctcaaga tttccaacaa
     1681 attttaaatg aagtatatag tcatcaagaa gcggctagaa tattgagcga actgccggaa
     1741 tatcaagaag aaacacttga aaaagccaag cagctgctta atcaagattt tttatttgag
     1801 gaggatcagg aatgaagatt ttaaggatcg agattgctgc ttttggaaaa tggcgtcaaa
     1861 aaagttttga cttttattca ggcaatcagc tgatctatgg gggaaacgaa gcaggaaaat
     1921 caacgattta tcaatttatt caagcaatat tatttggttt cccttcaaaa ggccgtaaaa
     1981 aaaaggatta tacaccaaaa gacggctcag cctatggcgg caaaatatgg cttaaacatc
     2041 ctgtatatgg tgaatttgct gtcgagcgct acaagcagca aaatcgcgga aaatcgaagg
     2101 tgtggctggg ggatcaagtg ggaagtgatg agttactaga aaaactgatc gcacctttga
     2161 cgaaagagtt attccagcag gtatttactt tccagcaaga gcagttgatg gaactagatc
     2221 atttgagaga aaaagagcta catgatgcgc tgatttcttt aggcatcacg ggcagttctc
     2281 aagtattcca aaagcggcaa gaatattatc agaaagcaca acaattattc aagaaaaaag
     2341 gccaaaaatt actaatcaat caaaaattaa atgaatggaa agagctgcaa gaaaaaattc
     2401 atcaaaaaca agaacaggaa gcttattttg gcaagttgat ccaacaagaa caggaatacg
     2461 agtcgcaaca gcagcagtta ttggcacaat taaaagaggc gaacaaacaa ttttcattag
     2521 ccagacagca agaacttaat ttttctttat atgaagaatg gcaatctctc aaaaaaaaca
     2581 gcactacagg tcttcctgat gagcaacttc ttttagatct ggagtctttc tccaaacggt
     2641 atcagcaaac aaatgagcgt ttggaagaat tagaagcaac actggaaaag catagcggga
     2701 tggatcagca gtcagccaga tattattttt atttagagca ggagcaggaa ctaaaagatc
     2761 tccagcaact tcaatttaca gcggaacaat taaacgaaga atggcaaaga ttacaaattg
     2821 agactagaga aacaaattca gaattgaaga ttttggaaca aaggtggcat tggcagaaaa
     2881 atccgttacc ggaagaattt ttatatgaag aagaatggaa gcatcttctg gctgaagaaa
     2941 gaaggattgc tgatctagaa agccagattt ctgtgcgtct ccaaatagct aaagaacagc
     3001 aagagatgat tgaagcagaa gtgaatgact tggaagagac ctatccagca ttattacaaa
     3061 atcagtcaga gatagcacca gtctcttcat ctaaaccgta tatgctgatg gctggcggag
     3121 caataatcat tgcaggtata tttgtaccaa tgcccttgaa agtgatcctt ttactaattg
     3181 gtgcaggcat gattggctat ttcttctaca aaaggcaacc aaaaattaaa actaaaaatg
     3241 atttctctga gataaaagga atgtggcaag aaaaactagg acaacttgat ctagccttag
     3301 ctcaattagc tgaagcagaa gaaaaacaaa agagaactgg agaagaaaaa cagattcttc
     3361 agcaagaagt atatcgtttt gcggatcatc ggcacttagg aaagatggat acgctcaaac
     3421 tcatgaaaga atatggacag gaagtcatag attatcagaa attatatggc gtatttgagc
     3481 ggaaacataa aagagaacag gaaatacaag aaaaactacg acaaatcgac caaaaatttg
     3541 agtttgttaa agaatggtta ccgatacaag ataagacaat ttcagaaaaa atggtattgc
     3601 tttcccgttt tatacaagag atggaacaga tgaaatttgc cagaagctat cagcaaaata
     3661 ctttattgaa gcaagaaatc aatcaattga aaaagaaaca aaaagtatgg atacaagaat
     3721 acagcgaaca attgactcaa gcaggcttat cttatccttc ggaagtacct gtctttttac
     3781 aaaaagccaa acaagccgaa cagcagatga aaagatacaa tgaattatcg gaaatgctta
     3841 ctccactatt tcctgaagag ctgacaaaag agaaattatc tgtaagactg cgccaactga
     3901 aagaaaagca atcccaactc cagcaagaag aaaaagaagc aagtgaaaat cggcaacgac
     3961 tccaacttca agtagaagag ttgcaaagag acggcacatt ggacgaactt tatcaacaag
     4021 ctagccggca aaaagcaaaa ttacaggaat tactggagga atggggagca cttgaaatcg
     4081 ccgggacatt cttaggtgat ctttcaacag agatgagtga aagacaatta ccccagttgt
     4141 taagagcagc aggtgattat ttaggtattt taacaaaagg aaattatcag aagatattac
     4201 tgaaaaatga tacactgact ttaacagacg gatatacaga atttcccatc tatgagttat
     4261 ctacaggtac gaaagatcag ctgattatgg cgatgagatt tgcttacctc tcattagaag
     4321 ctaaccgttc gatatgcccg atcatcattg atgatggctg gcttcattat gatcatcagc
     4381 gaaagtatca attagctgaa cttttagcta aatttggaga aaagtatcaa ataatttgtt
     4441 tttcttctga taaagaaatg gtaagctatt accatgagat gaaccaaatc gtaaaacaat
     4501 tagaaggagc aaataatgaa aaaaattcgt gatttagtaa tcgatgagca gtttgaacaa
     4561 tttgtcctga tcaaaagtgc agatgtaaga atcgctaaaa acggaaaaaa atttattgct
     4621 tttactttcc aagatacttc aggtactatc gatggtaaat actgggatgc ttcggaggag
     4681 gaaatcaata aatttacagc cggcacagtc gtttttctaa acggtaaaag agaggtttat
     4741 caaggaaacc ctcaagtaaa gattcttcat atgcgcttaa caagggtaga tgagccagga
     4801 gagcctagtt tatacatgga aagagcccct ttgaagaaag aagaaatgga agaagaaatc
     4861 aatcaattgt tgtttgagat caccaatgcg cattggaatc gtattgttcg ctatctactt
     4921 aataaatatc aaaaacaatt ttttgagttt ccggcagcaa aaagaaacca tcatgcattt
     4981 gccagtggat tagcttatca tactttgaca atgcttcact tagggaaatc aatcgccgat
     5041 gaatatcctg agctgaatcg agcattgttg tatgctggta tcattttgca tgatttagga
     5101 aaggtgattg aactttccgg tcctatgtcg acagaatata cgcttgcagg aaatttgatc
     5161 ggtcatctgg tattgattga cgaagaaata actaaagcct gtcttgcgtt gaaaatcagc
     5221 gaagctgacg aagatgttct cgtattacgt catatggttt tagcacacca tggcttatta
     5281 gaatatggct cacctgtacg tccaagaata atggaagcag aaatattaca tcaaatcgac
     5341 aatatcgatg cttcgatgca gatgatgttg acttcgattc gtcaaacaga accaggaaaa
     5401 tacaccgatc ggattttcgg tatggacaac cgaagtttct atgtaccaaa agatttatag
     5461 caaaaattta tttttttcat tcaaaacaaa aaaagcgaga cataagtctt cgcttttttt
     5521 gttttctttt attttgatga atcagttgtt tcttcagttg aagattcttt tgttgaatca
     5581 gttgattttg tgtcagaact ctttgttgaa gctgtagttt cacttgagtc tttcgtactt
     5641 gatgaagttg ttgtgaatgc actcaacaca ttttcaaagg catcatcttt gatttttaca
     5701 tttgcatctt ttaattcttc accgattact ttagttgtga atgcattgtc acttagttta
     5761 gtttcagtag cgatatcttt tagttgatct ttgtatttat ccatgtcatt gcctttattt
     5821 tggtttttca ccattttgac aacgtagtat tctgtcgcat aagaagtagg atttgttgta
     5881 gtaataacat cggagatctc accgtctttc aatttgaaag ctgcttcttt tacttctgca
     5941 ggaatagttg ttgtcgttga gtcgaattta actttaccgc catcttcttt tgtctctgta
     6001 tcagttgatt tgtctttggc taatttagag aaatcatctc catcatccgc tgattttttc
     6061 acatcttttg cttcatcttc acttgataat ttgatgattt gtgcttctac ttctggatgg
     6121 aaagatttcc aagcagtttt caaatcgtca tctgtaatat ctacgtgagc tttcaagcca
     6181 gcttcaaaag ctaaattgtt acgaatatat tctttataag tatctttcgt atatccagca
     6241 gcttctaatt gtgattcaaa agtatcacct aatgatttcg cttgtttgtc gtattctgca
     6301 tccacttgct tatcggttac tttatcgcca tatttgttat tgaatacttt ataaatgatc
     6361 atccgttgta ctaaggattg gttcgatgat tcaagttttg cttcatcata gaaatcagat
     6421 acagtgatcg ttccgccttt catcgttgcg atatctttat tagtatcgcc tgaacaagct
     6481 gctagagtta aaacagccaa cgcactagta gctgccaaaa taattgattt ttttttcatt
     6541 aaaatttgca ctcctgtaat tagttattat ttttatatat taactatacc atatcccatt
     6601 tcctttaaaa acgttgtcgt ggtagtttca aacaaaattc aaaatttcat cacattccta
     6661 ggaaatacgc aaacttttct cacttatata gttaaatctg ttgagaaaat aatcattatt
     6721 atatttgttc aggatactct ggcagatcag cttttagttt ttctacttgt tcattgattt
     6781 gtgtgatacg tggttcggct tggaatttaa aatcttcgat atcttttttg attgcttctt
     6841 ggaaagcagg aatcacagat tcagctgttt cttttgtttc gatcaatgcg cttttaaaat
     6901 gattcaggct gttatttaaa tcatttgtca gagttgttgc ttcttccaat tcttcgatca
     6961 gtttctttct tgtttcattc ccgctcctgg gcgccaataa cagacctccg acagtgccag
     7021 cagcagctcc aaataacagt cctttgaaaa agtttttaat catcgcagga cacctgcttt
     7081 acgattgttt cttgaatagc ttttagttct tccgatccat aactatcttg atgattgaca
     7141 aagtgaatag aaaaatcatc ctctttgctg tagcgaggga ctaaatggat atgtgaatga
     7201 aaaacagatt gataggctag ttcacggttg ttattcaaga tattaagacc tttcatttct
     7261 ggaaaagctt tttccaatgc ttgagcaatc ttaggcacac gcgaaaaaac ctctcctgca
     7321 agttcgggat catattcaaa aatatctgct acatgacgtt taggcacaac taatgtgtgt
     7381 cctttcgttg cttgagagat gtctaaaaaa gcatagacct tgtcgtcttc atatatttta
     7441 tagcttggga tttcttgatt gatgattttg caaaaaatac agttttccat ataaaacatc
     7501 ctttctataa agaataaata cttcagctta actttaccat aagttaaagc tattgtaagg
     7561 aaaaaaacaa aataccgtca ttttcttcgt ggtataataa ataaaaataa atggcggagg
     7621 aaaaagaatg agtttaatta tcgatcatgt cacagggggg tacggacatc tccctgtgtt
     7681 aaaagatatc agtttcgatg tacagcctgg agagatagta gggttgattg gactgaacgg
     7741 agcagggaaa agtacgacca tcaagcacat tatcggtctg ctggaagctt caaaaggaac
     7801 gatagaaatc aatcatctat ccttaaagaa aaatacggaa gaatatcgca aacaaattgg
     7861 gtatattcca gagactcctt ctttatatga agaattgaca ttgagagaac atatcgaagt
     7921 cactgcgatg gcctacgata tacctatgga tatagcaatg aaaagagcag aaaagttgtt
     7981 ggagactttt cggttatcta atcgtcttga ctggttccca gctaattttt caaagggaat
     8041 gaaacagaaa gtgatgatct tatgcgcctt tttgatcgag ccttctttat atatcatcga
     8101 cgaaccattt ttaggcttgg atccactagc tatcaatgca ttgcttgaat tgatggtaga
     8161 aatgaaaaaa gaaggagccg gtattttgat gtctacgcat attttagcta ctgcagaaaa
     8221 atattgtgat cgttttgtgg tcttacataa cggagagata cgagcagacg gttctcttga
     8281 gatgttgaga aaagaatttc agctgccgga ttcttctttg gacgaaatct atattgcttt
     8341 aacaaaggag caggcacatg agtagttttt ttcaaaaacg tttagcaaga catcaaaaga
     8401 aaatgatgaa atatatgcgt tatgtattga acgatcattt tgttttagtt tgtcttttct
     8461 tgattggcgg tgtgggatta tattattcta attggttgaa aaccctgcag ccaccgtttg
     8521 caattggagg gctcataatc tctgtatttt ggatgctctg tttattcgtg ggtaagatgg
     8581 ctacttttgc agaggcagca gatattgttt ttttacttcc taaagaaaaa gaaatgcaaa
     8641 cctatttgga acgagctttt cgctattctt gcatttttcc gttgatcttt ttgatattgg
     8701 catgcggatt ggccatgcca ttagtggtgg ttagtacgga gcaatccttt tcgatgtttt
     8761 tcgtctatct gatcatatta tggagtttga aattcagtca tctgcaaatc aagagagcag
     8821 aattgtttcg actagatcaa caagttattc gttattggaa attaggctgg tttttttctt
     8881 ctctgatctt tttgtttcta tcattttatt ggctaatatg ggcaggaata gcaggtgcat
     8941 tagtacaggc aagtctatat tactggtttt tgtggaagca acaagaaaaa ggattggatt
     9001 gggagaaaat ggtgcatgta gaagaacata ggttgcatca gatctatcaa tttatcaatt
     9061 tattcacaga tgttcctgaa attacggcaa agatcaaaag aagacgctat ttggatccta
     9121 tattgactca tattaaaaga gaatcaaaaa acacttatct ttacttgttt gcaagaagat
     9181 tggcaagagg ttctgatttt agtggtcttt atttccgatt agtaattata ggtgggttat
     9241 tgattgccgg agtagtcgac ttttatttta gtctgattat cggtttctta tttttatatt
     9301 tgatcggttt ccaactgtta cctttagcga accagttccg ctatatggtt ctcgttcagc
     9361 tttatccaat ccataagaat cagaagaagc aggcgattca gtatctactc acatgggtat
     9421 taattatcgc gtcggttatt tttggtttga ttgctgcagt cgtattagat gggcagcagc
     9481 gatgggttcc gctagcggct tatttgattg ttactatgct ctttgttaaa ctatatgtac
     9541 caactcgttt gaagaagatg tcagactgaa aacaaagctg tgtagggtaa aaatgagaat
     9601 gaaaaataga gcgttgacag taagaatgct gttcgttcta ttttttttga gaaaagctgg
     9661 ttttttgaat ggcaattaaa tgaaaaagaa cgtatatgtg cttgacgtgt agtgttcagg
     9721 cgactagaat aataagtact gcaaaacaaa gagtaagagg acgggagaat gcatgatgga
     9781 gtttcagtta gaccaagagt ggcggttacg tcccatcaaa ggcgacacag gaaaaacata
     9841 cataggcatg cgtgatgagg acaaagtctt tattaaacga aacacgactc cgatgttggc
     9901 tgctctttca agggaaggaa ttacaccaaa acttgtttgg acaaaacgaa cggggaatgg
     9961 cgatactttg acagcacaag agtggctgga aggtcgcttg ctgactccta atgagattgg
    10021 tcaaagaaac gacgtgattg atgttttgta ccatttacat cattcaaatt ctcttaaaac
    10081 catgctgacc cgtatcggtg gtcgtgtcat gacgccggag aagatgctgc aaaaatatga
    10141 aaaagaatta ccggaagaac tccagaaaaa tcaatttctg caaattgtcc atcgttttct
    10201 tcgtgagcag atcccgtcaa tagataagga aaacttatca gtagtacacg gagatgtaaa
    10261 ccatcgtaat tggttagtat gtaaaaatta tttatatcta gtcgattggg attcaatcat
    10321 gtttgcagac cctgcggtgg atattgcaac gattttagga aattatgtgc cactgtctag
    10381 ctggaatcag tggctgatca gttatggcat acgcccgact gacgaaatgc ttgaaaaatt
    10441 tcattggtat gcggccatga atttgctcca agaaatcacc agatattgca aacgtgaaga
    10501 tcatcgacgg atgaacgaag aaattttaca attaaaacga attttcagtg gctgaggttt
    10561 ggcccaaact ctgcggagat ttggccagac ctttttaatt agaggaaagg aatggagata
    10621 aataatgcgt gtccgaaatc gtccgggagc agcggaaatg ttagcggctc acccgaattt
    10681 tgtaattagt gatccaacat tgtggaaggg aaaatggaat gaattgtttg aaaacgatca
    10741 tccaatccac attgagattg gtatggggaa aggccagttc atcactggaa tggcaaaagc
    10801 ccatcctgaa atcaattata ttggagtaga aatgcaggtc agtgttgttt cgattgcact
    10861 agacaagttg atcgaacaac cgcttcctaa cctgaaactt ctacatgtgg atggatcagc
    10921 gttgacagag tatttcgctg acagcgaagt agatcaaatc tatctgaatt tttccgatcc
    10981 atggccaaaa aaacgtcatg aaaaacggcg attgacatat aagacttttc ttgctgtaga
    11041 tgaacagatt cttcgtccaa atggagaaat ccatttcaaa acagataatc aaggattgtt
    11101 tgagtattct ctggcaagtt tttctcaata tggcatgatc ctcaaacagg tatggttaga
    11161 tttacatcaa agtcaatttg aaggcaatat tatgacggag tatgaagaaa aattttcttc
    11221 aaaaggtcaa cgtatttatc gagtagaagc tcaatttcaa gataagacca aaaagaaaag
    11281 tctgtgaaac taactttctg ctttcataaa aacattaata tccataaata aatcccagaa
    11341 aatgaacatt acagatgtaa tgtattttct gggatttatt tgatttaaaa tgtatttatt
    11401 tcatcggttt ttccacaagg tggaatcaat tggaagcgag aaaatgaaaa taatcctttg
    11461 tcggtgatct tcaattcggg tatgacagat aatgttaaga aagaaagtgt caaaaaagga
    11521 tcaaaggttt tagctgctcc tagttgatgt gctttagtta ttaattcatt caactgttca
    11581 tagacgatcg aaaaaggttc ctgtgtcatg attccgccta taggaagaga aacacaagct
    11641 atttcctgct gtttgttgac aacggtcatc ccgccacctt tttgaatcaa aagattagca
    11701 gcatacagca tatcctcgtc gtttgtccca acgacaacta gattatgcga gtcatgggca
    11761 atcgttgtcg ctatcgctcc ttgtttcagc tgaaatcctt tcactatgcc taatccaatg
    11821 tttcccgttt gatggtgacg ctcgatcacc gcaatcttta attggtcttt ttcgattgaa
    11881 ggaatgaatc gttcgccttg tcggtccact ttttctacca aatctttagt cactagacta
    11941 ttcgggataa tttcgatcac atgggcttgt gaagaagaaa gaagcagctc taacgaatgt
    12001 ggatttaatg gttgagcgtt tagttttggc agacaacacg tatatgaatt tgaatgatca
    12061 gcaaataaat ggtcttggag ttgcccagat tgaacaacta atgttccctt atagaaaact
    12121 tcatgaattg gcagcttggt aagatcgtct gttaagaaga aatcagcttg atacccaggg
    12181 gcaatcgcac ctagtgtttt caaatcaaaa cattctgctg cattcaatgt tgccatctga
    12241 atggcttgta atggatcgat tccaagtgca atagcttgac ggacgatatg atcgattgat
    12301 ccttctttta ctaaatcatt gatcagttta tcatcggtga caaagaagca tcttcttgaa
    12361 ttttcaggtg tgatcgccgg tagtagcgat gccagatctt ttgcaacggt cccttctcga
    12421 accattaaat acataccaag atccaaacgt tctttagctt cttttgcagt tgtagcttca
    12481 tgatccgtcc gaatacctgc tgcaggataa acatttaact cttccatgcc gataccagct
    12541 gcatgcccgt caattttttt cttatgctgg tgcatcaatt ggatcttttc taggatgtct
    12601 gtttcattat ttgctactgc ttgatagttc atgacttcag ctaagcctaa aacttcgggt
    12661 ttttgtaaaa aaggggctaa tttttttgca tttaaatttg caccattggt ctcaaaaggg
    12721 gttacaggta cacaagaagg tagcataaaa aagatattca tgagtgtttg tcttgcatct
    12781 tctatcataa actcaagacc tgtactccca gcaatgtttc ctatttcatg gggatctgtc
    12841 actacagtag taactccatg aagcaaggat acttttgaaa attcgcttgg tgataaaagt
    12901 gagctttcga tatgcacatg gccatcgatg aatcctggca ctagatattg cccattagca
    12961 ttatgtacgc tttttccttc gtattcaccg attcctacga tcattccttc gcagattgct
    13021 acatcttttt ccatgatttc tttcgtaaag acgttgacga tcttagcatg cttgatcact
    13081 aggtcagcag gctttttttt acttgctact tcgatatatt tccttaatgt tgtttccaat
    13141 ggagtgcctc ccaagataaa tttttataag tataacataa attcggtttt tgccaatatt
    13201 tttttgtaaa ttcattttat ttgaaacaaa aagatcaaaa agacgtatgt tttttgtgga
    13261 ggggagaata atggaaataa aacgattatc ggatcaaatg ataagtgaaa gttttgccaa
    13321 acatgcccaa gacgtatata tattgccagc ttcagaagta ttttacatct attttcgcta
    13381 tggaaaaaaa cgaacattct ggaagaaagt ctcttcggca gatggacagc aactgatctc
    13441 aagatttaag tatttgggac agatggatat cggagaaaaa agaaaagctc agttaggtgc
    13501 atttacttat tctttagaaa aacaggaagt tcgtctgcgg ttgtcttctg taggagatta
    13561 ccttcaacga gagagtttag tgattcggct attatacggt acttttgaaa caccccatcg
    13621 ttgctttagt tctcgagatt ttgcaactat tttacagaaa accgtcagac gtggattata
    13681 ccttttcagc ggaccagtcg gatcaggcaa gacttctcta atgtatcggt tggctttgga
    13741 agaaaaattg caagtgatta ctatcgaaga tcctgtagag atagaagaac cgttttttct
    13801 gcagctacag atcaatccga aaatagcaca gacttatgat acacttttga gactcgcatt
    13861 acggcatcgt ccagacttgc taatcattgg cgagataaga gatgaatcaa ctgcacatgc
    13921 tgcggtaaga gcagcactaa cagggcaccg ggtttttgca accgtacatg cacgcaatct
    13981 tgaaggtaca ttggtcagaa tccatgaact gacaaagaaa aaagaagagc tatctgaatg
    14041 tttagctggt gtcatttatc aagaactttt ggaagatgcc aatcaccatc cttctgtttt
    14101 atggagttat caatttatgg aggaagaatc agtaaaaaaa tcatgggagg aaagttatga
    14161 agaagctcag gaaaatattt ggcagtcaga actattctaa aggattaaca agaagccagc
    14221 aaaaccagtt tgcacaaacc atgggagaac tgcttttatc tggttttagt ctgcaagaat
    14281 ccctttattt attactcacg atccgttcgt ttccacctgt tattttggca aatatccaac
    14341 atcagctaaa ggaagggcaa gcgtttagtg aggtactttc gcaaatagga tatggagcag
    14401 agcaactggt acagatcgag ttagctgaac gtcatgggaa tctgccgaca actttgttgg
    14461 aaattgccaa acagtttcgg cttgttgaag gatttcgcaa tgatttgaaa aagctgcttg
    14521 catatccgat gctattgctc acctttttgc taggtatctt gtttagtatg cggttgttcg
    14581 ttttgcctca gcttattggt tcaggaatgg tcatgcaaga gcattggggt attcagctga
    14641 tacaagtctt tcattggtat gttctactat tcatcctagc ggttactcta ttggctttac
    14701 tagtaaggta tcgtctgatg aagttgtctc ctgttgatca gagtgaatgg ataagcagac
    14761 aattattgat tggaccgata tattccttgt atcaatcggc ttatttgtca ctagagattg
    14821 gaaagctttt tcatgaaggt ttcgaattaa ggcaaatcat agtctgctta aaggaaacga
    14881 aacaagaaag tctgattcag catcttgcct ctaagttgat tcaaggattg gaggcaggta
    14941 tgtcattggc tgagcagttc cagaattatc gttttctatc agaagaattc agtaaaattg
    15001 ttttacaggg agaggcaaag ggaagtttag gtaaagaact gattttttac agcgaatgga
    15061 caagaaaaca gcttttcaaa cgtcttcatc actggatgca ttttattcag ccaatcattt
    15121 ttttgggcgt agcggctttg attttattag tttatgccgc tgttttatta cctgtttacg
    15181 gaagcataga ggaggttttg ccatgaaaaa gagaaaaata tcttattata gcggttttac
    15241 attgatcgaa atgctaatcg tcttactgat catcagtgta ttagtgttgt tgttcgtccc
    15301 taatttgtcg cgttatagga atcatgtaga ccaagaaagt cgtgaagcga tcatccagtt
    15361 ggtagacaca caaaaagagc tctatgcgct tcaaaataat ggaagagttc ctactgtgga
    15421 ggaattgttg aatgaaggct atatcaaacg agaacatgca gaaatctatc agcggccttg
    15481 aaggtaaggt tttagcatca ggatatacgt tgtttgaatc actcttactg attttattac
    15541 ttacaacgat cctttctttt ccagttctcg cattttctgc atggaagaat gaattggcag
    15601 tctatcaatt tttcggccaa tttgaaaaac gaatctattc tacgcaaaaa atcgctattg
    15661 tgaatcagat ccaaacaggc ttttattgga atagtgaaga gaatcaaatc atttttcatg
    15721 ttccgaatcc agataaaata aattgggaag tattagatat tcctgatgag attaccttga
    15781 aacgccatgc ttctattact tttgctgcta gaaccgggaa tgaaagttca ctaaaagctt
    15841 atcaatttta ttgggaagaa aaagagcgga cgattacgta tcaattccag atagggagtg
    15901 gtcgctatac aaaaagaatt gaataaaaca ggatttgttt tgttggagtc ccttacagcg
    15961 cttatgatcg cttctgtctg tgtctactta gtgagctttg gccagctgtt tttgcttcag
    16021 gcagaaaaga aaagtgggga ggaactgcta tttattcgaa tgatttatga agatatcaag
    16081 acgcagcgga tgtatcacaa aagaccagag cgaagggaag aaatgatccc atacgatatc
    16141 caaattgtcg ataataatgg agtaatcgaa aaagtgtttg tcaatatgga gaaagaagcg
    16201 atcagtattg aaaaaacaag taattccagc atttacgctg attgaatgtc taattggact
    16261 gatgatattg agcagtctat tgctcacatt caatcttttg attcagcaaa ccatccaagt
    16321 aagtgaattt ttgcagcgta aagatcaaaa agagtggcta atatttttag cacaattaga
    16381 agaagaactc aagagcagtc acaatgtctc tgtgaaaaat cagcaactgc actacacgat
    16441 cgaggataaa cagtatatca tcgaaagata tcaggcaatg atacgtaaaa gaagaacttc
    16501 cggcggacat caacctatgt taacaagtat cactgagctt caattattgg aaaaagaaca
    16561 cacaatttct ttttatgtac actttgaaaa tggagaagat ggatatgcca tatggacaaa
    16621 aaacgatcag tagaaaatgt tataagggct ctattttatt ttctgtcttg cttttatttc
    16681 ttttatgtac gtttttattg atggcagcat tagaaagcta ccgtttgtca atggatctta
    16741 atatacgaat caaaaattat tatatagcaa aaataatgaa gcagatggct ttatcggaac
    16801 agtctacgct tgccgaaagt tcggaaggag ttttttacta cacaactgga tctgtaacat
    16861 atcagtgggt acaacagtcc ctattcttag aggtggaagt ctcaccgttt atctttcggt
    16921 tcacagaaga agtaaaaaat gatacagata ctagtacaga atgatggaat gttcgtgaaa
    16981 acgccctctt ttctgttata acactgtttt taataaaaaa atgtttgccg tttcacaaat
    17041 aaaaaacagt gaaaacgatt gaaattttgt caattgttcg atagaattaa acaggtgaaa
    17101 aaattgttat aatatatcta gaaatgaggt gcaccatgtt accagaaaaa atggaacaag
    17161 ggtttaatca aaacctggaa gctattcagc ttttacagaa tgcattggga acttcctttt
    17221 tagaagccta tgtcgaaaat gcagaaaatt taatagatga ttatcaagta cgtgtggtgg
    17281 atggagtgcc gacaaaagag acaacgcaac ggatcacagc tctttatgaa gagttgaaga
    17341 aactttcttt cgagccagaa gaatggcgca gactgtctca attactttta ttgaagggca
    17401 gccagacaga acatttgcag gcgaaccatc aactcactcc tgatagtatc ggttttttat
    17461 ttgtttttct tattgaacaa ctatacacaa ataaaaaagc accggttaag attctggata
    17521 ttgcggcagg aatgggcaac ttgttattaa cagttctttt aaacctatcg aatgcggggt
    17581 accaaacaga aggaattggt gtggatattg atgatacatt gttggctgtc gcagcttcta
    17641 caagtgattt gacacaagca aatgttcaat actttcatca agacgggttg caggaattat
    17701 taatagatcc tgtcgatttt gcaatcagcg atttgccaat cggttattat ccgaatgacg
    17761 aaaaagcaaa agagttttta acaagtaccg aagaaggcca tagttatgca catcatttat
    17821 tgctagagca atcaatgaaa tatgtgaagc cggatggttt tggtttgttt ttgatgccga
    17881 gtggattttt agaaacggat caaagtgaag aaatcaaaaa atggtttaaa gaagaaggtt
    17941 atcttcaagg aatgattcaa ttgccagatg aattgttccg taataaacaa tcgcaaaaaa
    18001 gtattttgat cttacagaaa aaaggaccac aagcacaaca agtaaaagaa gtcttgcttg
    18061 tgaaacttgc ttcattaaaa gaaccagaga aggtgacgga atttttcaac gagtttaaaa
    18121 actggaaatc atctgctctg taatcattca acgagaagga gagtcatatg tctaaaacaa
    18181 ttgcaatcaa tgctgggagt tcaagtttga aatggcaatt atatcaaatg ccagaagaaa
    18241 ccgtagttgc gaaagggatc gtcgaacgta tcggtttaca agattctatt tttaccatca
    18301 agtacggcga taatcaaaaa ttcgaacaaa tcatggatat cgaaaaccac gaagtagcag
    18361 taaaaatgct gttagatcag ttgattgaac tgcaaatttt aagctcttat gatgaaatca
    18421 ccggagtagg gcaccgtgtt gttcacggag gagaaactta tggggattca gtagtgatcg
    18481 atgagaaagt aatggaccgc attgctgaat tagcagaatt tgctcctttg cataatcctg
    18541 caaacttaat ggggatcaaa gcgtttaaaa agattttacc tgacattttg agtgtggctg
    18601 tattcgatac atctttccac tcaacaatgc caaagcaaaa ttatttatat agcttaccgg
    18661 tgtcttatta tgaagatttc aaagtaagaa aatatggctt ccatggcaca agtcatcgtt
    18721 atgtgtcgga acgtgcggca aaattattga acaaaccgat cgaagaacta aaaatcatca
    18781 cttgccactt aggaaatggt gtatcgatca cagcagtcga tggaggtaaa tccatggata
    18841 cttctatggg attcacacca ttagctgggg tgacaatggg tacacgttca ggtgatatcg
    18901 atccagctgt tctcccatat ttaatggaaa aattagagat tgatattgat gaaatgatca
    18961 atgttttgaa caagaaatct ggtctgctag gcctaacagg attgtctagt gatatgcgtg
    19021 atcttgaaaa gaactatgaa aaagaacata ttcgtttagc ttatgacatt ttcgtggacc
    19081 gcatccgaaa atacatcggc ggttatgtga caacgatgaa tggtgttgat gcaattgtct
    19141 ttactgctgg tatcggcgag aatgacggac atgtacgtag tgaaatcatc aaaggtatgt
    19201 cttggtttgg ttgtgaaatc gatccagcgt tgaatgaatt gcgtggtgaa gaagtagata
    19261 tctctacaaa agaatcaaaa gtaagagtat tagtgatccc aacagatgag gaactaatga
    19321 ttgcccgtga tgtggagcgt ctgcgcggct aagccaaagc attcgttagg ataaaagatg
    19381 aaacgcatgc aagagggaat tcttgcatgt gttttttttg cggagaagac cgcaaaatgg
    19441 taatttaact tcaatttccc ttctttttta caggatcttc ttagggaatt ttgcttggaa
    19501 aagtggtcaa aagggaaaag actggcattt tatttattga ttttcaggaa aatcccaata
    19561 ttttattttc attcatcttt ataagggaca ttacatatgt aaaaagaaaa aacattttta
    19621 gatcagcctc gtgggctgtc gactttattc ttcgctttat gtttagctag aaaaccgatc
    19681 cgaaacattc attgatcgaa taataaaaac ttgattttcc acctgtactt tgcctgaatc
    19741 gtgaaagcac aggtatgaaa atcaagtttt ttgttgcgaa aaaacgtcct agtcaatcag
    19801 ttggccgagc ggctggcggc tgatctgctc ctgatcaata acgagtgctt cttggaagtg
    19861 ttgctgcaaa aagtagcggt catggctgac tgctaggagc gttccttgat agtcttgcag
    19921 catttcttca atttcttctc gagcatagct gtcgaggtga ttggttggtt catccaaaat
    19981 caggacgttg attttttcta aaaagagttt cagcaacgcc aaacggattt tttcaccacc
    20041 ggataaatct ttgatccgtt tacccacatc gctttgataa aagccacttt tggctaattg
    20101 ccgtcgagcg acttcttcgt ttccagtcaa tgtgatggcg taagccagca aacggctatc
    20161 gggatcggca aactgcagct gctgcggcaa ataaccgatt tgcaagctac tacctaattt
    20221 gatggtgcca ccgtaaggct tttcctcacc gagtaataat ttgagtaagg ttgattttcc
    20281 tgcaccattg tcgccgatga tggctaaatg atctccacga tagactgcaa aactgctgtc
    20341 tttgaatagt acttgatcag cgtactgctg gtgcaagttt tgaatgatga aaacttcttt
    20401 gccagatttt ccaccattag acaagctttg cagccgattt ttcgggggag gtggcggttt
    20461 gacgagtgtc agtttcgcca ggcggcgttc cagttctttg gcttttttga agaaggactc
    20521 attgtctcct tcatgtgccc actgacgaaa acgacgaatc atgactttca accgctggac
    20581 ttctttttgc tgcagttcgt aattttttgt cagctcagcc aagcgagctt ctttcaagac
    20641 tcgaaaacgg ctgtagtttc cgggatactc aatcaatgca ccttcttcca gttcccatgt
    20701 ttttgtggtg acttgatcga gaaattgccg atcatgagaa acgaccagaa ccgatttttt
    20761 tgtattctgt aaatagtttt ccaaccagcg aatactgtta agatctaaat gattgatcgg
    20821 ttcatcgagt aaaagtaaat cggcatcggc tgctaaaatt ttggctaaag cgactttaac
    20881 tcgttcgcct ccgctaagtt ctgttaaccg tgcttcctga cggtgtggaa tccctaagcc
    20941 ttgcaagatc gctgcaatcc ggtcttctaa tacgtaaccg cctagttctt caaagcgatg
    21001 ctgcagctca ccgtaacggg ccagacactt ctctaagttt gtatctggtt ctgtcatcaa
    21061 tgcttcgtag cgactcattt gctgtttgag gtcctgtatc tcagcggcag aatggagcaa
    21121 gtagtggttg acggtcgctt cggaaactgt aagttcttgt tcgaccgtcc cgatctttaa
    21181 gccttttttg cgactgataa caccgctgtc gacaccttct ctgccggtca agatttgtaa
    21241 aaaggttgat ttgccagttc cgttgatacc gactaaggca attttttcac cagcttcaat
    21301 ttgacaggat aaatcctcaa ataatggatt cccgccgatt gtttttttaa tagaaattgc
    21361 ttgtatgatc atgatctctc tccttgagac cactcaaaaa ggccatggtt atccatggcc
    21421 tgtgttattt aaactcagat tatcatgaga aaaatacctt gagatggcca ttgattttac
    21481 gtgaattcaa ctgttttgga caaacttgtc ccgtcaacag caactgtaaa accaattgca
    21541 tggctaaatg cgatatggta tcgttggtaa ttttcattac ttgttatttt agtcatgcgc
    21601 atcatagtat tttccctcct tcgtgtagtt gtcttttcca gtgtacctga aagtgtcaga
    21661 aagttcaact agcgctgaca tttacttaat atttatcgat gtgtaattct gtcagagagc
    21721 tttttttatc tgactgaggg taatgtctcg tggaccacat cattgtattc tgagctaaac
    21781 cagcgttcag gcgaaatcat atggtgtttg ataaaaaact ttgtctacat gacttccttt
    21841 tgccatttgc ttaataccgt tgcattaaac agctcttctc tttggaaatc tcaccaacat
    21901 cgcgttcagg agtatcgttg tggttgcaat ggcaagcggt agcctttcat caattcttga
    21961 atgatttacc gaatgtagca cttgccttct cgcggtaccc gaacccgaaa taaaatttta
    22021 gtttggcgat aaaatcggta tccgccatcg atacttcata ttttttcgta tcagattcat
    22081 cggaaacttc cacattgacg aaccaataag ctttggcatg cttaggtcgt tttaaaccac
    22141 accagggcga ctaatcgccc tggtgttcac ttttttttat tttacttgaa ctcattgact
    22201 gttttccctt tattttaaac attataaaga ccgatgagtg cttgccgcgt cttttgtttt
    22261 tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg caggtcgtct acgtgttaag
    22321 ctttggtgtg gtctttagct ggcaattggt tttctaaatc agttcggaca acaagcacat
    22381 cgcaagctgc gtttctaata acatattcag aaacagaacc aataaataaa cgttctacag
    22441 cattcaatcc tgtggctcct aacataatca aatctacttg attatcttct gggatttctc
    22501 tggcaataat ttgttttggt gagccatatt ctacgacaga ggttacatta ttcaagccag
    22561 ctttcttcgc attgctttca taatctgcca atgtttgctt tgccatttca gtagcttgtt
    22621 ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg
    22681 ctaatagaag ttctccgtta tttcggatag ctacattgac cgccttttta aaagctaatt
    22741 cagcttcatc agaaccatct acggcaacca tgattttttt gtattgttgt aacataagca
    22801 ttcactcctc aagtcaatcg tattgtactc ttttattgta gaacacttta gggaaaaaga
    22861 gaattattta ctgctttggt tcagcaattt tttttgcaaa tcggatactg aaggtggcag
    22921 aaacaataat tacgatcagt aaaaagacca aggcaataaa gcgttgattg aaaaaagctg
    22981 ttaaaatacc cattacgcct aaaacagcat atgcagctcc gtaaaattgt aggaatcctt
    23041 gatttttatc atttttgttg atcaatacaa aaaagatatc ctgtttatta aataaataat
    23101 agctaacgaa taataatact aaagtggcga taatcattaa aatcctaatc atgaacaaat
    23161 cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa gagcaaattg ctttattctt
    23221 taaaagatgg taatctccgt taatgccgtt gtttgtcctg tagtattgat cccgcgaatt
    23281 caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa atgataaggc atgttaccag
    23341 cagtgacaca agacgggtcc caagatatca ataaaaatgt tcggtcaaca cacaaagagg
    23401 acaattcgaa catcacagat ttcccacctc tatagtagca tatgaattta aaaagagcaa
    23461 tgagagagct cgcagaaaca gaaccgtttt cttgtctagt tttttggagg atgctttttc
    23521 cactctttta tacggtaata ctgttgaccc agagcctgtt catatttccc tgtatctttt
    23581 ggctgataat actgagcatt cttcagttta tctggtaaat attgctgatt gacccaagcc
    23641 tgatcaaaat gatgaggata ctgataaccc acgccacggt tgagtgattt cgcacctttg
    23701 taatggctat ctctcaaatg atcaggaaca tctccagctt tgccctctcg aatatcagca
    23761 agtgctgcat ctaatgccat gtaggcagaa ttggattttg gcgacaagca aagatcgacg
    23821 acgacgtctg ctagtgggat acgtgcctca ggcaatccca atttttcagc agctaataca
    23881 gcgttcactg tacgagccgc agcagcagga ttacctaaac ctatatcttc ataaccgata
    23941 accatcagac gccggcaaat acttgccaga tcacctgcct caactaaacg ggcgagataa
    24001 tgaagtgcag catccacatc acttccacga atcgattttt gaaaagccga aataacgtca
    24061 taatgagcat ctccattttt gtcatgggta agagcttttc tttggacaca ttcttcaatg
    24121 atcgacagtg taagatgaat ccttccttct ttatctgaag gagtcgatag tgtagccagt
    24181 tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga
    24241 gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc gttctttatc ccttaaagca
    24301 tgttcgactg ccaactggat gtcttgttct gttaatggtt tgacttcaaa gatctgtgta
    24361 cggctgcgga tcgctggatt gatggtaata taaggattct cagtagtcgc tccgatcaag
    24421 atgattcggc cactttctaa atgaggaagc agaaaatcct gttttgtttt atctaatcgg
    24481 tgtacttcat ccaataagag gatcactgta ccactcattt ttgcttcttc cgctacaact
    24541 tgtagatcct tttttgaatc tgtagcggcg tttagcatgc gaaatgcata attagtagag
    24601 ccggcgatcg cacttgcgat actcgttttt ccagtaccag gaggtccata tagaatcatg
    24661 gaagagagca tgcgagcatc gaccatgcgg cgtatgattt ttccttcccc gactagatgc
    24721 tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc gataagccaa tggctgttgc
    24781 ataggatttt cctccttctt gtttttacgt cccttaatta tatcatattt agcaagatgc
    24841 ctagaaagaa cagccttctg ttgttttgtt ttcatagctt atccaactaa tattgtgtat
    24901 gataaagtag gtgagaaaaa tgacggataa acaaaagata gatgaatttt tttggaataa
    24961 aacgacgcca gaagtggcac gggatttatt ggggatgtat ttggagcatg acacaccaga
    25021 aggaagatta gctggatata ttgtagacgc ggaagcatac ctaggtccag aagacgaggc
    25081 cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg
    25141 tacgatctac ctttacacga tgcatactca tcggattttg aacatcatta ctcagccaga
    25201 agggattcct caaggagtaa tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat
    25261 gagtaaaaat cgtggcggaa aaactggtcc agatatcagc aacggtccgg gaaaattagt
    25321 ggaagcatta gcaattcctc aagaacttta tggtcagtct atagcagata gttcgttacg
    25381 cttagttttt gagaaaaaaa agacaccaaa aaagatcaat gctttgccta gaatcggtat
    25441 cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta
    25501 tatctcttta caaagaaaaa accaaatcga gaaagattgg ggttggagga aagaaaatga
    25561 aaaagaagga tctattaaca tatttagatg aaataattga aaaacaagtg acagactatg
    25621 atgtagcttt agattgggat acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg
    25681 cagaaaataa agcacaattt gagattgatg atgcagaagg gatcgtttcg caagaaccaa
    25741 ttatcgaatt tgaagacggc gtacttcttt ttaatcctca gaaatctgtt tttgatgaac
    25801 gcaattatct agcagtaatc ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg
    25861 attctttagt ggaatatcta aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt
    25921 ttttggatga agacgatgac gaagctgtat tcgagcttca ctgggataat cagcaactag
    25981 cagaattagt agaagtaaaa cagcaaaatg aagcagatac ttatttaccg tacccaagtt
    26041 attaaggagt gacagatatg aaatggaatg aagtaaaagt agaaactgaa agtgaagcag
    26101 tcgaagcagt tgctaatatt ttgatggagg caggggcgag tggtgtcgcg attgaagatt
    26161 ctttagatgt tgaacatttt cagtcggatc aatatggtga gattttagat aaaaatgatt
    26221 ttacatcgct gaaagaaggc gcactagtca tggcttattt tccggaaacg actttcttac
    26281 cggaaatctt gccttttatc aaagatcgta tcacacaatt acctgagttt ggtctagcta
    26341 ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag caactgggca acagcctgga
    26401 agaaatacta tcatccggtg cgtattacgc gaatgctgac ggttgcgcca agctgggaaa
    26461 actaccagac agaagatcct ctagaaaaaa ttatcacact agatcctggt atggcatttg
    26521 gcacaggcac tcatcccacg acttccttga cattgacagc actagaaaca gtcttgcgtg
    26581 gtggtgaaac attgctagat gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat
    26641 atcttggtgc aaaagatgtc tatgcctttg atttagacga agtagccgtt cgttcggcga
    26701 aagaaaatat ggatatgaat gaaatagcca aagatgtcca tgtatcagct aacgatcttt
    26761 tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa cattttagca gatatcatct
    26821 tattgatgat cccagatgct tggcgtttac tgaagcaaac aggcacgctg atcgtttcag
    26881 gaatcattga agagaagaag caaatggttc tggatgccat gttagaacaa gggtttatcg
    26941 tggatcaaat tttccagcaa aaagactggt atgcaattat tttgaaaaaa ccagaggtgg
    27001 aatagatgca gcggtatttt ttaaaagaat catatgaatc aaaagactat tttgttttag
    27061 aaggggaaaa ctatcaccac attgtacggg ttatgaggat ggaacccgag gatgaagttt
    27121 ttttggcatt caatgatcaa atagccattg ttgcagaaat cacggagatt actgaagagg
    27181 ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta
    27241 ccatagcgag tggctatcct aaaggggaaa agctggattg gatcgttcag aaatccactg
    27301 aattaggagc gcaagctttt cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg
    27361 caaaaaaacg tatgaaacga caacaacggc ttgaaaagat tgctcaagag gcagcggaac
    27421 aatcccatcg gcagatcgtg ccatcagtag agttattgga aacgaaacaa gatttatttg
    27481 agcggctaga tgattatgac gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg
    27541 aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc agatagtcga atcttagctg
    27601 tctttggacc agaaggtgga ttatcccgcg atgaagtcga ttattttgta gaacacaaag
    27661 ggatactttg tggattagga ccaagaatcc ttcgtacaga aacagcacct ctttatttat
    27721 taagtgctgc tagctatcaa tgggaattaa tgaatccttc tttcagctaa cgttcttcat
    27781 taataaaaga gtatcgacag aaagaattga aaaaatagtc aacaatttgt ataatagaaa
    27841 caattattta tacaatggat acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt
    27901 tccgtaaaaa aacgtataac tgcacggatt ttgggacaat tatcaaacag atatttgtcc
    27961 cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt
    28021 ttaacgggac ccggtgtcat aaagctcgtt agtcaataca tgggtcccga acatgtagca
    28081 tttgttgaga aagcatgcga atacgcgact gctgcacatg acggacagtt tagaaaatca
    28141 ggcgaaccct acattattca tcctatccaa gtcgcaggta tattagcaga tttaaaaatg
    28201 gatccccata cagtggctac aggcttctta catgatgttg ttgaagatac agaaatcact
    28261 ttagaagatc tgagagaaga atttggcgat gacgttgcta tgttagtgga cggcgtaacc
    28321 aaattaggga aaataaaata taaatcccac gaagagcagc tagcagaaaa ccaccgaaag
    28381 atgctgctag caatggctca agatttacga gttatcatgg tcaaattagc tgatagacta
    28441 cataacatgc gtacgttgaa gcacctgcga gaagataagc aaagaaggat cgctcaggaa
    28501 actttagaaa tctatgcgcc tcttgcacat cgtctaggga tcagccggat caaatgggaa
    28561 ttagaagata cagcacttcg ttatctaaat ccaaaacagt attaccgtat cgtccacttg
    28621 atgcagacga agagagaaga acgtgaaaaa tacgtaagtg gtactgttga agatattcga
    28681 atagcgacag aagagctggg gatttttgca gaaatctatg gacggccaaa acacatttat
    28741 tcaatctatc gtaaaatgaa agatcagaaa aagcagttca acgaaattta tgacttgcta
    28801 gctattcgag tgatcgtaga ttcaatcaag gattgttatg ctgtattagg agcaatccac
    28861 acaaaatgga aaccaatgcc cggcagattt aaggattata ttgccatgcc aaaagctaat
    28921 atgtaccaat ctttgcatac taccgtgatc ggtccggcag gaaatccggt agaaattcaa
    28981 atccgaacac aggaaatgca tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat
    29041 aaagaaggaa aaaatgaaaa agtagaacca gatggtatga cgaaacaatt aagctggttc
    29101 catgagatac tcgaacttca agacgaaagc tatgatgctt ctgaatttat ggaaggcgta
    29161 aaaggagata tctttagtga taaagtctac gtcttcacac caaaaggaga cgttactgag
    29221 ttaccaaaag gatccggacc attggacttt gcatacagta tccatacaga tatcggtaac
    29281 aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc ttgattacaa attgaaaaac
    29341 ggagatatca ttgagatcat gacttctcca aattcatttg gcccaagtcg cgactggttg
    29401 aaattagttg ctactagcaa agcaagaaat aagatcaaac gtttcttcaa agcccaagat
    29461 cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca aatgtattac agatctagga
    29521 tttacgccta aagatatttt gacgaagaac aaactgcaag aagcactcga tcgttttaat
    29581 tatcaaacag aagatgatct ctatgcagct gtagggtatg gagaagttag ccccttgacg
    29641 atggccaatc gtctgactga aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa
    29701 gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat
    29761 gaaggtggcg ttgtcattca aggggtagaa aacttactaa ttcgtatcag tcgctgctgt
    29821 aatccgattc ctggtgatga tatcgttggt tatatcacta aaggcagagg gatatccatt
    29881 catcgtcgag attgtccgaa tgttcagcct gacaaaccaa atgtagcaga acgtttgatt
    29941 gaagtcgaat gggaagatac atcgaataca cgaaaagagt atgatgcaga tttggaaatt
    30001 tacggctata atcgttcagg cttattgaat gatgtacttc aaacagtcaa tgcgctaacg
    30061 aaaaatctca acagcgttga agcacggacg aataaagata aaatggcgac gatccatttg
    30121 acggttggta tccagaattt atcccaccta aagagtatcg tggataaaat caaagcagta
    30181 cctgatgtct acagtgtacg ccggacgaat ggataggagg acagatatga gagcagttgt
    30241 tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta
    30301 cggcctactt gttttattag gcattcatga aaccgatacg caaaaagatg tggactatct
    30361 ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag
    30421 tgtagaagat gtgggaggac aaattttaag tatctctcaa ttcacactgt ttgcggatac
    30481 gaaaaaagga aatcgaccaa gctttgtgga agccgcacga ccagaaacag ctattccttt
    30541 atacgaatca ttcaatgaag gaatcagaag tcgcggcatc acagtcgcta ccggacaatt
    30601 cggagcagat atgtcggttt ctctcgtcaa tgatggacct gtaacgatca ttattgattc
    30661 taaaaacaaa taaagtaatc tggttttcac tagtaaaaac tatgaaacac gtcgcttaag
    30721 ttagactgat aaactctaac ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc
    30781 agcagtcgga aaaaaagtta agcttaaatt atacaagtta ttttttatag gaggttgctg
    30841 aagatgaaag aagtaccagt agtagatatc aaaatcacag atttaaaaaa attgtatcaa
    30901 aaaggataca acatgttgga ggagttacgc catgaagcgg acgctcccgt cgtcaaagca
    30961 aagattttca ataaagaagc tatcacgatc tacggatcat cagcagcaaa agtattttat
    31021 gatccaagga atttcaaacg aaaaggtgct atgccaaaat tagtattgaa gacacttttt
    31081 ggtcaaggtg gagtacagac tttggatgga gctgctcatc accatcggaa aaatattttt
    31141 atggatctaa tgacaccgga aagaatggaa gattatcatc gtatcttaga taagaatctg
    31201 acacaagcac ttgaagcaca gcatggtcaa tttgaactat ttgatttaag taagatggtt
    31261 ttctttacat ctatttgcga gtgggcaggt atcaatttat cagcgatttc taaagatgag
    31321 gtcgaaaaat tagcagaata tcaaatctct atgatcagcg gaacgtttac ttctccgatt
    31381 gatcacataa aaggtgtcga aaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt
    31441 gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg tggcattata tgcttttgcc
    31501 aatgccacag atttagatgg tcagctttta cctctggaag tggcagctgt tgaattattg
    31561 aacatcatcc gtccaacggt tgctctaact gtgtgggcgg cattgatggg acatgcactt
    31621 ttcagtagac cagatttgta tcagcaatta aaaaatgact tttcgacgtt acaagatccg
    31681 tttattcaag aaatgagacg ctattatcct ttcttcccaa tgctacctgc gatttcgctg
    31741 aaagaagtag aagtagatgg ttatcgtata ccagaaggca gttgggttat tttagattta
    31801 tatggaacag accatgatga acgaacagtt gaagcgccag attcctttat gatcaaacgg
    31861 tatgtaggga aagcaaaaga tatttcctat aaagaagaat atgagatgat tgcacaaggc
    31921 gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat ggatcactct tcatagtcta
    31981 cgtgtgtttt ccgatcagct agtaaataag tttgagttct ctgtacctga acaagattgg
    32041 acgattccat tcaatcagtt tccgacttat ccgaatagcc gagcgctact ttacaagaat
    32101 tagattagca taaacttatc gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt
    32161 catattacta taaattgata aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg
    32221 tttgataatc ttcattggat aaatgcaaat ctaaagcttg gacattgtca gatacttgct
    32281 ctggacgctt agctccaggt ataactacag acactcgtgg attcttcatg taccaagcta
    32341 aaacaagttg cgcaggagtg gcttggtatc ttttagcaat ggggcgaagt acatcgacag
    32401 cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg atttcttttt cgaggatctc
    32461 cttcaccaaa ttgtttttcc tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa
    32521 gaggaaaata gggaacaaaa ctgattttgt ttttttcaag ataagggaat aattcttttt
    32581 cagcctgccg atggatcaaa ctgtatttat cttctactac atcgacatat ccatctgcat
    32641 tcgcttcttt caattgttct aacgtgaagt ttgacacacc gactgctcgg atttttcctg
    32701 cttcttttaa ttcgtgcaat gtagcaactg attcattttt tggtgtgctt tcatccggaa
    32761 agtggatata aaaaatgtcg atgtaatcag tttgcagacg ttttagtgca tcttccaccg
    32821 attgtttgag aaactcagga gaattatcaa atgttcgccc tttatttggt acatgagcag
    32881 cttttgtagc gatgacgaca cgtgagcgat catattcttt ttccttcagt acctcgccaa
    32941 tcagttcttc ggaacggccg tttccgtaag cataagcggt atcgatcaaa ttgatcccgc
    33001 tattcaaagc tgtacgaact acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat
    33061 tatgtcctcc aacggcattt gctcctaatc caagctgttc tgcgtagact tgtgaatgtc
    33121 cgattcttat ttctgccata gctgattcct cctttttgtc tagtctaaaa caaaatacca
    33181 aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca gatatctctt gactttgaga
    33241 ctggttttta gtaggttata agtaatatcg aatgtcaatg aaagagcgag taagtttttg
    33301 aaactttaca agagaggatc atcgaggctg aaaatgatct aaagtttcat tagcctaaag
    33361 acactctgga gatggacgag tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga
    33421 aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat ttcattcacc cttgttcatg
    33481 atgaacaagg gtgatttttt tatttacata gctaagaaag ggagagaaaa ctatgagttt
    33541 ccaaagacca aaaggaacaa atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt
    33601 cgaagaaaca gcacgtttat tattcagaga ttatcagtat aatgaaatgc gtacaccgat
    33661 ttttgaacac tatgaagtga tatcccgaag tgtaggggat acaacggata tcgtttcaaa
    33721 agagatgtat gatttttatg acaaagggga acgccatgtg acgcttcgac cagaaggtac
    33781 agcaccaatc gttcgttctt ttgttgagca taaattgttc ggcccagagt atgcgaaacc
    33841 gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt
    33901 acgtcaattc catcaaatcg gagtagaagc atttggatca gaaaaccctg caactgatgt
    33961 ggagagcatg gtgatggcac ttgatttctt caaacagttg gggatcacgc aaatccggct
    34021 agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga
    34081 ctatctttta ccttttgaat cagagctgag tgaagactct aaacgccgcc ttcatgaaaa
    34141 tccattacgc gtattggaca gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc
    34201 atctatctta gattatctaa gcgagcctgc tcaaaaacat tttgataccg ttgtttccat
    34261 gctggatgca ttggctgttc cttatgaaat cgacagtaat atggttcgcg gactagatta
    34321 ttacacacat acgatttttg aaatcatgag tgacgcacca aaaatggggg cacaagcaac
    34381 gatttgcgca ggcggacgtt atgacaatct agtggaagaa ctaggtggtc cttcaacacc
    34441 tggcttcgga tttgcaatgg gtatcgagcg attactcttg actatggagg cagaggaagt
    34501 agttatccct gcatttaatg agttggatgc ttacgtggtc gcactcggag acgaaacaaa
    34561 tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga
    34621 cttcatgaac cgtaaagcaa aagcgcaatt taagacagca gataaagcga atgccaaatt
    34681 agtattggtg atcggtgaag atgagcttgc tagtcaaacg attaatgtca aagcaatggc
    34741 aagccgaaaa gaaaaaagtt ttccattaga agagatatat gaacattttg atgaagtcta
    34801 tgatgaaatg actttattcg agtaggagga aaaaaataaa tggcacaaag aacgatctat
    34861 tgcggattag tttccgaaca acaattagaa caaacagtta cattaaaagg atgggtacaa
    34921 aaaagacgag accttggggg agtaatcttt atcgacctac gtgatcgtga aggaatcgtt
    34981 caaatcgtct ttaatcctga aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc
    35041 agtgaatacg tgatcgaagt gactggggaa gttagaagac gcggagaaaa tgcagtaaat
    35101 cctaaaatgg ccactggtga attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa
    35161 gcaaaaaccc cgccatttca aattgaagat gatcaagcaa tcagtgacga aatacgtttg
    35221 aaatatcgtt atttggatct gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat
    35281 gcgacaacaa aagcgattcg tcattattta gatgataatg actttctcga tatcgaaaca
    35341 ccttacctag ggaaatctac accagaaggc gcaagagact acctagttcc ttcacgtgtc
    35401 catgctggac atttctatgc gttgccgcaa tctcctcaat tgtttaaaca attgctgatg
    35461 aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt tccgcgatga agatttacgt
    35521 ggagaccgtc agcctgaatt tacgcaagta gatattgaaa caacgttctt aacagcagaa
    35581 gaaatccaaa cttacacaga gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc
    35641 gaagtcacat tgccatttcc tcgtatgacc tatgatgaag caatggctcg ctacggcagc
    35701 gacaaaccgg atacacgttt tgatatggaa ttgatcgatc taagcgatac agtcaaagaa
    35761 gtagaattca aagtcttcca aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat
    35821 gctaaaggcg ctgcagatcg ctactcacga aaagacatgg atcagttagg tcaatatgta
    35881 ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt
    35941 ccaattgcta aattcatggg agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa
    36001 ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt
    36061 gctatccgga cacgtttagg aaaagaatta ggactaattg atgaatcgaa attcaatttc
    36121 ttatgggtaa ctgattggcc acaatttgaa ttttcagaag aagaaggtcg atatgtctct
    36181 gctcaccatc cattcacgat gccaaaagaa gaagatatcc cgttattggc tgaagaccct
    36241 gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg gttatgaact aggcggaggt
    36301 tctatccgta ttcatactcg tgagcttcaa gaaaaagtac tagaaactct aggctttaca
    36361 aaagaacaaa tggaagaaca atttggtttc ttgttaacag ctttagatta cggattccct
    36421 ccacatggcg gtattgcatt aggattggac cgttttgcaa tgttattagc cggagaagat
    36481 aatattcgtg aagtcattgc ctttcctaaa aacggaaaag cagcagatgt gatgagtgat
    36541 gcaccaagta tggtttcgcc tttacaattg ttcgaattga atattgatgt aacgaatata
    36601 gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt
    36661 gatttaattg aaaaacactt tataatgaag ccataacata taaagcgggg cttttccatg
    36721 aaaaaaacat tcgaaaattg ggcttatcat gattacacga ccaagctttc ggtatctatc
    36781 gtttatgcgt tgatcgcatc agtcgcattg aatttctttt atcagccagg acatatttat
    36841 tcaagtggta ttactggtct agctcagatt ttgacgactt tgtcaaaatc actgatagga
    36901 ttcgaagtac cggtatcaac aactttgtat ttactgaata tacctttgtt tgtattggcg
    36961 tggttaaaaa tcgggaagaa gtttacaatt ttcactttta tcacagtcac tttgacctcc
    37021 ctttgtatcc atattttacc ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg
    37081 tttggggggg ctgtgatggg actaggtatt gggctagcgt tgagaaacgg cctatcatcg
    37141 ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa caggaaagtc gattggttct
    37201 atttcgatct acttcaatgc attgatcgtt tttgttgccg gaattctatt tggctggcaa
    37261 tatatgtttt acagtacgtt gtctattttc gtcagtggaa aagtaactga tgtcgtttat
    37321 actaaacaaa agaagatgca ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat
    37381 gaaatccaaa actgtatgcg tagagggatc acgatcatca atgaagcaga aggtgcttat
    37441 aagcatgata agcagacagt attgatgacg atcgttacac gatacgaact gcctctactt
    37501 agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa gtattgcaga aaacgtccag
    37561 atacttggtc gtttctatga ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat
    37621 actgctgtag acagacgttc gtttgtctgc agtttttttc taccatagta gaatcagcaa
    37681 agaaaaactg cttttttatt tgaaattttc ttaaagttga aaaaaagcat gtttattttt
    37741 ttaaatttat gggatgatag aaaaaaatag tagagaaagg acacgattct acttgctatt
    37801 ttcacttaat acccgtatat tacgaatgaa aaaagtttat gaaaagttgg aatattgaat
    37861 ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact gggtttgatg ggctccgttc
    37921 attagctgtg gtgggtgtta tcttctatca ccttctgcca actagtatga aaggcggcta
    37981 tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg atcacggatc tgctgcggca
    38041 ggaatgggaa caaaatggaa aaatcaatat ttggcaattt tatgtcagaa gaatgaagag
    38101 actctatccg gcacttgttt ttatgcttgt gacagcttcg gcttatatca ctttgtttca
    38161 gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa
    38221 ctggtggcaa atcaataacg gtttatctta tttcgatcga tttgctaatg agtcaccgtt
    38281 tacgcatatc tggtctttag cagtagaagg tcaaaactat ttggtttggc cgatcttgtt
    38341 tgtattgttg atggtatttg ttcgtaaaag aaaatggatc ttttatactg tgttaggtac
    38401 atccttcctc tccgccatct tgatgatgat cttgtataca ccaggaggcg atccaacccg
    38461 tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt
    38521 tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa caagcgaaaa atattctgaa
    38581 tatcgctgga ggaatctcgc ttttggcact gattctttgc ttcttctttt tagatgatca
    38641 ttacaatttc atctattacg gaggaatgct cctagtgagt ctgttatgcg tagtgctagt
    38701 tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg ctgacaaatc cagtatttac
    38761 ttggattggc aaacgaagct atggcattta tctgtatcag tttccagtta tgatttttta
    38821 tgaagcaaag gtgactgata ttgccgatca tgttttgttg catactttag tggaaatcac
    38881 attgattcta atcatcagtg aactttctta tcgatatgtg gagcgtccgc tagcacgttt
    38941 tgattatggt cgtacattcc aagtgatgaa aggatggttc agcaaaccga tcatcagtaa
    39001 gagcaaacca tgggttgttc caggcacatt acttgtagca attgcactcg ttggtttcgt
    39061 tacggcaccg aaaaacgctg taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga
    39121 aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa aacaccacta gttcggttga
    39181 ccaagcgatc cttgataagt acgagttgtc caaggcacaa gggaataagg cacaaaaatt
    39241 agaactaacg gcatttggtg attcagttat gttggatgct gcaacggatc tacaagaact
    39301 ttatccaaaa gtagtggtag atggagatgt cggacgacaa ttatatgcca gtgtaccgta
    39361 cattgaaaaa ttaaaggaac aagatttgtt gaaagacact gttctgatcg gactaggaac
    39421 gaatggctca ttctcggaag ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa
    39481 agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat
    39541 gcttgaacag atgacgaaaa aatacgacaa tatgacgctg attgattggt acagctacag
    39601 taatgaccat agtgactggt tttatgatga tcaagtccat ccaaatcctg aagggatgac
    39661 acattacatt cacttagtca gcgaagcctt gctaggagga agttcaagta cgaacagcag
    39721 tgagacaaac gtgacagaat gatagctaca aaaaacttca ggcaaaaaca tttgtttttg
    39781 cctgaagttt ttttatattt accgtgtttt taacattcat atagattcag gaagcaaaac
    39841 ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc
    39901 ttctgctttt gttgcttcgc ttcttgaaga aaatacttct gcatggatca tcttaacagg
    39961 tcttcttttt tgaagacgag tatatttagc accagtacca ttgttatgtt cctctaatcg
    40021 tctagaaaga tcggttgtat atccaccata aaatgtctga tctttacaga ataatacgta
    40081 aaaataatgt tcatttgcca tagagcatct cacgaatctc tgcagtgtat tctccatcca
    40141 tattgtaagt gatcaatggt ggagcgactt tgaatccgtc taatttgcct tctttgattc
    40201 cttcaactaa caaaatattt gcctcttttc caggttttgg gtaaacaaat tggatccttt
    40261 taggtgcaat tcgatagtct tccatgacct gaatgatttc taagaaccga tcaggacgat
    40321 ggaccatagt aaagcgtcca tttgttttta gtagcttact tgaaatatcg atcacttctt
    40381 ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg tttattaggg ttcgttttat
    40441 ttgttggcag ccctttgaaa taaggtggat tacagacgac taagtcacaa gaattatgtc
    40501 gaacgacaga taagctgtct ttcagatcga tggtgtggac agtcatttgt tcatttaaat
    40561 ggttaagcgc gatactacgt cttgccatgt ctgctagacg aggttgaagt tcgatagcgt
    40621 cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa
    40681 ggtcaacaat cttccctttt ttaggtacac gggaaaagtg agcaagcagc acagcatcta
    40741 aagaaaaaga aaacactttg gaactttgga taatcttgat atcttctgcg tataattggt
    40801 cgattcgttc gtcttcgtga agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt
    40861 attatactat ttttattaaa aagaaccaag gtggcttgtc aaacctctat tttttatgca
    40921 tactatgacc ataagaaggg aaaggaagag actatgtttt atcgatttat gcgcggggta
    40981 gttcgagtac tattagcctt agttaatgga aatgcccgct atgagaataa agaagtttta
    41041 ccgcaagatg agaattacat actagttgct ccccatcgta cttggtggga cccattatat
    41101 ctggcggtag ctgcagcacc caaggaattc agctttatgg caaaagaaga actgttcaaa
    41161 aatccagttc tgcgatttat attaactcga tccaatgctt ttccagttaa aagagaaaat
    41221 ccaggaccaa gcgtcattaa aacacctgta aagattttga aatcgacaaa tttgagtttg
    41281 atcatgtttc ccagtgggac aagacatgct acagaactta aaggcggaat ggcattgatt
    41341 tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc aaggtccgtt gacacttggg
    41401 gatctgttca agcgtcagag agtgacagtg cgtttcggcg aaccaatcga cttatcagat
    41461 atcaagaaaa cggataaaga aggtatggcg gagatcgaac gacgaacaca agcagctttt
    41521 gacaaattgg acaaagaagt gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat
    41581 gataagttta tgcgtgggcg gtcttttgaa tctactgtcc tgttcagttc agactttttc
    41641 aatgaaaacg agggagagac gtcaagtctg ccctcttttt gcgttccaat atgtaaaata
    41701 gaagacaggt gtcatgtaat ggatcaaagt gttataataa ttagaaggac attttttgag
    41761 gtgattgaat gcgtttttta catacagccg attggcatat cggaaaaaaa ctacaaggtt
    41821 atgacttgat tgaagaccag cgtcatgtac tatcttctat actagatatc gcgaaagaag
    41881 aaaaagtaga ggcaatcgtg attgccggag acttatatga taggagtgtc ccagctgttg
    41941 atgccgtaga attatacaat gaattgatgg tcgattggaa tttaaaggaa aagatcccga
    42001 tttttgctat atcaggaaat cacgatagca gtacgagact ttcagcgggt acgccatggt
    42061 tcagttattc gaactatttt ttgcgaacac agctagcaga agcatttgaa ccagtcgagt
    42121 ttaaaaacgt acaatttttc tttcttcctt actttgaacc aattgcagcg agattatatt
    42181 ttgaagatga caatatccga acgatcaagc aagcggtagc caaggtgata gataaaatgc
    42241 aggagctatt tgattcagaa aaaaaacaag tattgattag ccattttttt gtaatgggaa
    42301 gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg gttggattcg atccctggtg
    42361 aactacttac accgtttgat tatgtagcac taggacattt gcataatcaa gctgctttga
    42421 gacaggataa tgctcgctat agcggttccc cattgaaatt ctctttatct gagatcaacc
    42481 aaaaaaaagg cgtatggatc gttgacctga caacaggcct ggagttgaat tttagggaga
    42541 tcgaacccct gcatgatatt gaagaaataa cagcgagttt caaagaattg ctggatcctg
    42601 ccttttatca gtccgtcaat cgggagaatt acatctatat ctatctcact gatagaacga
    42661 tcatcccgaa tatgatgaac cagcttcggc aaatatatcc tcgtattttg gggattgaac
    42721 gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa agtactagaa atgaaagatc
    42781 ctcaaaaatt agcagaagca ttttttagtg aaatgacgga agagcagcta actgcaaacc
    42841 aagaaaaatg gctcggagaa acgttgcaag aaatcatgga aatggaatga tcaagaatga
    42901 aaccgaaaaa attacaactt aaaaattttg gtccttttat caatgagacc gtagattttt
    42961 cacgtttgac agaagcaccg ctttttttga tcagcgggaa gacaggagct ggcaaaacga
    43021 cgatttttga tgggatcacc ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg
    43081 gaaaagagat gcgttctttg tttgctacac cagacgaaga aacaagcgtg actttttctt
    43141 ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac
    43201 gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac gttgaccatt tttgatggga
    43261 aaggaaaaga aataaaacaa atcacaaaac gtacagaagc agatcagttg atcagagaac
    43321 tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt actaccccaa ggagaattcc
    43381 gtaatttttt ggtttcatca agcagtgata aagagactgt tttacgtcat ttattcggta
    43441 ctcaattttt ccaacagttc aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa
    43501 aattggatca tttagagcaa gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag
    43561 aagaggaacc agcagcaggg tttgaagccg ttttagccca atggacaaac catcaggcaa
    43621 tactcgaaaa acaaatcacg gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa
    43681 agcaactaga aaattcctac tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa
    43741 cgatattaat aaacaagcaa aaggaattag ctgctcaaaa agacgaaata gaggaaaaga
    43801 aacgatggat tgggtattat gaatttacgc aacgattagt cgaaccaatc cagcgcctac
    43861 aagaaataaa agttgaaaaa atagaactgt cacaaaaaag tagtgagcaa acaaagaaac
    43921 tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc
    43981 gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa caaacagatg atggtgccaa
    44041 ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca attaacaatt tcattggata
    44101 aagaagaatt gaatgccgag aagctgtatc aaaatctcgc tatctatcag gaaagaactc
    44161 gaattcttca agaaaattta ctggatcagg aaaaactgca agaagatcgt cttgcttttg
    44221 gacagttaaa atatctagat gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca
    44281 ctgggtcgct cgcacagata agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa
    44341 atagtcagct attgaaagaa cagacagaac aagcagaaca gctgaaaagc caatgggcaa
    44401 agcagcaaat tgctcgtctt cagctttttt tgcgtccagg ggagccatgt cttgtttgtg
    44461 gatcaacgga acaccccata caacaattaa aacacgaaca gccaaataag gaagatatct
    44521 taaaagtaga acaacaactg gcagaagctg agtctgctgt agaaagaact cagcaaatca
    44581 ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca attatcagat ttagaacaaa
    44641 aaatcatggc aacgaatata caattggctg aacaagaaga acacttgcaa caggctttga
    44701 aaaaggcatt tcctgagata acagaacaaa aaacaaaaac aactcttgta gtaatcgaac
    44761 aatctttaga tagacgaaaa agcaagatgg ccaatgcaag aatagagctg gaagaaataa
    44821 gacaacaagc agaaaagctc gaacaacaat tagctgtcta caaacagcag cagacagaaa
    44881 aaaataatca gctccagcaa ctgcaaggag aaatcaaaag tttagaacaa caattgcaag
    44941 atacggatag taactgttta gaagaggaga tacgacgact tgaaaaacag ttagaagaag
    45001 actcgaatca actagctgtt gagcagaaag caggagtgca attgttgcaa gatcttcata
    45061 cgcttcggac acagcttgat cttcaacatg aacaaataga aaaaatagat gaaaaaatag
    45121 aaaaggaaca agaaagaata gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg
    45181 aagaaatctg tgcatttgca caacagcaag cacaatataa agaaaaccaa cgtacagtta
    45241 cttcttatca tgaagagcaa ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc
    45301 agctaactga gacattccca gatacagaag agatcagcca acagttagaa gaaataagac
    45361 gtatgatcga aataaagcaa gaaaagatat acggtttaca agaacaaaaa attagtaatc
    45421 aaaaaatcta cgatgaatgt ctttcgattt atcacacaag ccagacgaaa ttagatgagc
    45481 ttacgcaatt acagcagctt tcgcaaacat tcaacggaga aaatcctaag aagactagtt
    45541 tggaacggta tgtgcttcag gtttatcttc aagaagtgtt acaagtagca aatgatcatc
    45601 tccaacgttt aacgaaaaat cgttatcaat ttgagctagc ggataccatt ggcagttatc
    45661 gagggaaaac cggattagaa atcaatatct atgatgatca ggcaggtatg tctcgaagtg
    45721 cgcataccct gtctggcggt gagagtttta ttgcagcact ttctttagca ttagctcttg
    45781 ccgatgtgat ccagacacaa gctggtggtg tagcaatcga tgcattgttt attgatgaag
    45841 gatttgggtc attagatgaa gaagctttgg aaatggcaat ggaagcttta gaaacgatcg
    45901 aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag agaattgaaa gaacgagtat
    45961 tacaacaaat aagaattgat acagaaggta gcggacaaag caaaatacgc tatcaacttg
    46021 gataaaaagg gagattggta actatgaaat ggagtattcc agaaaaagtc attgcacgtg
    46081 gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg
    46141 tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt tgatttggat ggtacagcaa
    46201 aagagataga ttactgccaa tgtccctatt gggaggaaca taaatattgt aagcatacag
    46261 ttgctgtgga actttattta cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc
    46321 caatgctaga accggtaaaa gcaacaagtg agggagaaat cattgcaaaa ggtttttctc
    46381 gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt gattattgaa taccaagtag
    46441 aaacgattga gactaatcag tatcacccag agctttctat tttgccactt tacttgcgta
    46501 tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa aaacatctat gattttttac
    46561 aagcttacac agaaaaagaa acttacactg ttaataaaca gtaccagttt cgtctagata
    46621 aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca tcttgcagga agtgctcaga
    46681 cgcatcaatt attaggtacg aacggcttac aagtcaaagg aaaactagat aaaagatatc
    46741 tgcttctccc agtagaacaa gcccgcttcc tgttagagca gatgaatcag actacacgtt
    46801 tgcatctgca gataaatgaa caaaacgatc aaggaataca attttccgat caaaacaaac
    46861 ctttatcatt caaagtggaa aaatttggag aagattattt gctgcaagca ttgaatgatt
    46921 ttgatttttt ctttatgcat tatcagtggg gagttatcca agggacattt tattctctga
    46981 cgaaatatca acaaacggtt tatcaaactt ggaaacaatt gattcgccga ttggagaaac
    47041 cagaagtagt gttcaagaaa aaagaattac cgtctttgtt taaagagatt atccctctgc
    47101 tgtcagagat cggcgacgta gctattgccc ctgcagtaga agaggaagta gcagatatcc
    47161 ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt
    47221 tcgtctatgg cgaagtcatc tattcgactg acccgaagca tgaggtctcg acagaacagg
    47281 cttttcaggt gttacgtgac acagtagaag agaaacgagt acttgatcta ttcagaatgt
    47341 atcgctacca gaaaaatgaa actggttatg aacgtccatt gccaagcggg gaagaactat
    47401 atgccttttt ccgcacagaa ctatctgtct tccgtcagtt tggtgaagtc agacttggaa
    47461 gaaaattgcg agagttgtat ttagatgcgc ataaacatca accgaaaatc gaagtaaccg
    47521 agaccggttc atggctcgat attcgattcg atgtgacagg gatagaggag caagaaatcg
    47581 atcatgtcct gcaaagcttg ctgagaaatg atgcttttta tacattagaa aatggtcagg
    47641 ttctttcttt tgattcagaa gaatttcaac aaacaagcca agtgttgcaa caattacggg
    47701 aatcgatacg tacagaagaa ggaacgatcc atgtacctaa aaatcaagga ttgatcattc
    47761 aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc gttccaaacg atggtacaag
    47821 atctcattca tccagaaaga taccaggcac agcttcccaa aggattgaat gcaacgatga
    47881 gagattatca aaagcaaggt tttcgatggt tgaagatgtt aggtcattat cagtttggcg
    47941 gtattttagc cgatgaaatg ggacttggga aaacactaca gacaattgcc tttttacttt
    48001 cggaaaaaga agaacggaaa agcttctctg ctttgatcgt agcgcctgcg agcttaattt
    48061 acaactggca ggcagaggtg agaaaattcg ccccttcttt aagtatccaa gtaatcaatg
    48121 ggaataaaaa agaacgtgaa gaattactgg ctaaagacac agatatccga gtgacttctt
    48181 atgccagctt aagacaggat ctagctaatt atcagtcgca aaaaattgat tatttgattc
    48241 ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac tgcacaagca ttgagagaat
    48301 tagctgtacc tcagcggttt gcactgagtg ggacaccaat cgagaataat ttagaagaat
    48361 tgtggtcatt gtttgcaacg attatgccag gatttttccc gactaaaaca aaattcaggg
    48421 aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt tattttacgg cgagataaac
    48481 aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa tctatattct gcacttacag
    48541 aagaacaaaa aactgtttat ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa
    48601 tggattctga agcattcaag aaaaaccgga tcgggatact tgctggtctg acaagactgc
    48661 gacaaatttg ctgcgatcct cgtctgttta ttgaagatta ccaaggcggt tccggcaagc
    48721 tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa caaacgaaga gttttgctgt
    48781 tttctcaatt tacagggatg ctgactatct tgcaagaaga gttagccgag ctagggatct
    48841 cgacatttta tttaagaggc agtacgaaac cacaagatcg gttaagtatg gtagacgcat
    48901 ttaatgctgg cgaaaaagat gtttttctga tttccttaaa ggctggggga actggattga
    48961 atctcacggg agcagatacg gtcatcttgt atgacctttg gtggaatcct gccattgaag
    49021 aacaagctgc tggacgtgca catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta
    49081 tgatctctga aggaacgatt gaggaacgca tggattcact ccagcaagaa aaacgcgaac
    49141 tgttccaaaa agtgatccaa ggtaatgaag aacagttgac gaaactgact gaagaagaca
    49201 tccgcatgat tttaagtgta ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt
    49261 aatgggtaag aaagcctttc taaagaagcg attgtttatt tgatttaaaa aatagcaagg
    49321 aggctgggat tccattttat cccagcttcc ttgctatcat ttttttattt ctatttttaa
    49381 aggttataca aagattgtct tcattgtctc taacagctga tcaaaagaag aaatctcgat
    49441 atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca
    49501 gttagcactt tttgcaccta gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc
    49561 ttctggaagc atatgaaatt ctttctcagc tagctgaaaa atctcttttt ctggtttttg
    49621 gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga atccaattgt tcaattgaag
    49681 ttgcttcagt ttttttgtct gatggtcagt cggcccattt gtgataattc ctaatgggac
    49741 attcttttcc ttcaagaaat cgagggtttt tttgacctcc tcatgcaagc agatattatc
    49801 aagttcttcc tcgtaaattt tttgaaataa gaggccatct tcttccgtaa tatgagggta
    49861 gtctaaatct tttagtgatt ggctgatccg atgtgctcgc atatattcta gtgtccaatc
    49921 tcctgccatt acttttggga agttttcatc actatgatgg cggaaccgga tatatagtgg
    49981 atgcatatct gcatctgaaa caagcggaac gactcttttg accgcgtttc gaaatggctg
    50041 ctgctgatca tacatcgtat catcaacatc aaacacaact gatttaatca aaattttaca
    50101 cgcctttcgg ttttcattga ttttttcaca aatccttgac tattgtaaca gaaaaaattg
    50161 tctaaaaaag ctgataaata aaggtttttc ttactatatt tctatttcct tgacagaata
    50221 aggtttttat atttcagttt ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg
    50281 aacaaattaa tcattat
//
LOCUS       DAGCYL010000003        49561 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.003, whole genome shotgun sequence.
ACCESSION   DAGCYL010000003 DAGCYL010000000
VERSION     DAGCYL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.003"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(176..1462)
                     /locus_tag="MB216_000094"
     CDS             complement(176..1462)
                     /locus_tag="MB216_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBT4524960.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="MB216_000095"
     CDS             complement(1455..2723)
                     /locus_tag="MB216_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBT4524961.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="MB216_000096"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="MB216_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBT4524962.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="MB216_000097"
     CDS             complement(3495..3941)
                     /locus_tag="MB216_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4524963.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4034..4279)
                     /locus_tag="MB216_000098"
     CDS             complement(4034..4279)
                     /locus_tag="MB216_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBT4524964.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4394..4972)
                     /locus_tag="MB216_000099"
     CDS             complement(4394..4972)
                     /locus_tag="MB216_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBT4524965.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5049..5579)
                     /locus_tag="MB216_000100"
     CDS             complement(5049..5579)
                     /locus_tag="MB216_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBT4524966.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="MB216_000101"
     CDS             complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="MB216_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBT4524967.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6436..6831)
                     /locus_tag="MB216_000102"
     CDS             complement(6436..6831)
                     /locus_tag="MB216_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBT4524968.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6828..8240)
                     /locus_tag="MB216_000103"
     CDS             complement(6828..8240)
                     /locus_tag="MB216_000103"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBT4524969.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="MB216_000104"
     CDS             complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="MB216_000104"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBT4524970.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(8859..9098)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9095..10555)
                     /locus_tag="MB216_000105"
     CDS             complement(9095..10555)
                     /locus_tag="MB216_000105"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBT4524971.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10725..11882)
                     /locus_tag="MB216_000106"
     CDS             complement(10725..11882)
                     /locus_tag="MB216_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBT4524972.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11916..13286)
                     /gene="radA"
                     /locus_tag="MB216_000107"
     CDS             complement(11916..13286)
                     /gene="radA"
                     /locus_tag="MB216_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBT4524973.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13349..13876)
                     /locus_tag="MB216_000108"
     CDS             complement(13349..13876)
                     /locus_tag="MB216_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBT4524974.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14106..14912
                     /gene="proB"
                     /locus_tag="MB216_000109"
     CDS             14106..14912
                     /gene="proB"
                     /locus_tag="MB216_000109"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBT4524975.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14909..16156
                     /locus_tag="MB216_000110"
     CDS             14909..16156
                     /locus_tag="MB216_000110"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBT4524976.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16232..16576
                     /locus_tag="MB216_000111"
     CDS             16232..16576
                     /locus_tag="MB216_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4524977.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16693..17019)
                     /locus_tag="MB216_000112"
     CDS             complement(16693..17019)
                     /locus_tag="MB216_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4524978.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(17392..18593)
                     /locus_tag="MB216_000113"
                     /pseudo
     CDS             complement(17392..18593)
                     /locus_tag="MB216_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            18710..19225
                     /locus_tag="MB216_000114"
     CDS             18710..19225
                     /locus_tag="MB216_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4524979.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(19424..19870)
                     /locus_tag="MB216_000115"
     CDS             complement(19424..19870)
                     /locus_tag="MB216_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBT4524980.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(19917..21395)
                     /gene="msr(C)"
                     /locus_tag="MB216_000116"
     CDS             complement(19917..21395)
                     /gene="msr(C)"
                     /locus_tag="MB216_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBT4524981.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(21749..22378)
                     /locus_tag="MB216_000117"
     CDS             complement(21749..22378)
                     /locus_tag="MB216_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBT4524982.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(22362..22826)
                     /locus_tag="MB216_000118"
     CDS             complement(22362..22826)
                     /locus_tag="MB216_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4524983.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(23313..24581)
                     /locus_tag="MB216_000119"
     CDS             complement(23313..24581)
                     /locus_tag="MB216_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBT4524984.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(24812..25549)
                     /locus_tag="MB216_000120"
     CDS             complement(24812..25549)
                     /locus_tag="MB216_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4524985.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(25616..26185)
                     /locus_tag="MB216_000121"
     CDS             complement(25616..26185)
                     /locus_tag="MB216_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBT4524986.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            26313..27185
                     /locus_tag="MB216_000122"
     CDS             26313..27185
                     /locus_tag="MB216_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4524987.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            27527..28399
                     /locus_tag="MB216_000123"
     CDS             27527..28399
                     /locus_tag="MB216_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4524988.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            28396..30354
                     /locus_tag="MB216_000124"
     CDS             28396..30354
                     /locus_tag="MB216_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4524989.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(30605..31456)
                     /locus_tag="MB216_000125"
     CDS             complement(30605..31456)
                     /locus_tag="MB216_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4524990.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            31579..32046
                     /locus_tag="MB216_000126"
     CDS             31579..32046
                     /locus_tag="MB216_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBT4524991.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(32309..32791)
                     /locus_tag="MB216_000127"
     CDS             complement(32309..32791)
                     /locus_tag="MB216_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBT4524992.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(32891..33754)
                     /locus_tag="MB216_000128"
     CDS             complement(32891..33754)
                     /locus_tag="MB216_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBT4524993.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            34352..35200
                     /locus_tag="MB216_000129"
     CDS             34352..35200
                     /locus_tag="MB216_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4524994.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(35371..35712)
                     /locus_tag="MB216_000130"
     CDS             complement(35371..35712)
                     /locus_tag="MB216_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBT4524995.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            35798..36505
                     /locus_tag="MB216_000131"
     CDS             35798..36505
                     /locus_tag="MB216_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBT4524996.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            36492..36815
                     /locus_tag="MB216_000132"
     CDS             36492..36815
                     /locus_tag="MB216_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBT4524997.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(36904..37941)
                     /locus_tag="MB216_000133"
     CDS             complement(36904..37941)
                     /locus_tag="MB216_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBT4524998.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(38388..39371)
                     /locus_tag="MB216_000134"
     CDS             complement(38388..39371)
                     /locus_tag="MB216_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBT4524999.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(39787..40557)
                     /locus_tag="MB216_000135"
     CDS             complement(39787..40557)
                     /locus_tag="MB216_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4525000.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(40550..41485)
                     /locus_tag="MB216_000136"
     CDS             complement(40550..41485)
                     /locus_tag="MB216_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4525001.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(42079..43728)
                     /locus_tag="MB216_000137"
     CDS             complement(42079..43728)
                     /locus_tag="MB216_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBT4525002.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(43923..44819)
                     /locus_tag="MB216_000138"
     CDS             complement(43923..44819)
                     /locus_tag="MB216_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4525003.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(44812..45492)
                     /locus_tag="MB216_000139"
     CDS             complement(44812..45492)
                     /locus_tag="MB216_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4525004.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(45493..46275)
                     /locus_tag="MB216_000140"
     CDS             complement(45493..46275)
                     /locus_tag="MB216_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4525005.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(46268..47185)
                     /locus_tag="MB216_000141"
     CDS             complement(46268..47185)
                     /locus_tag="MB216_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4525006.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(47188..47472)
                     /locus_tag="MB216_000142"
     CDS             complement(47188..47472)
                     /locus_tag="MB216_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525007.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(48173..48793)
                     /locus_tag="MB216_000143"
     CDS             complement(48173..48793)
                     /locus_tag="MB216_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525008.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(48874..49284)
                     /locus_tag="MB216_000144"
     CDS             complement(48874..49284)
                     /locus_tag="MB216_000144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525009.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            49314..>49561
                     /locus_tag="MB216_000145"
     CDS             49314..>49561
                     /locus_tag="MB216_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBT4525010.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNG"
BASE COUNT        14338 a        10458 c         8808 g        15957 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
     4081 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
     4141 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
     4201 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
     4261 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
     4321 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
     4381 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
     4441 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
     4501 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
     4561 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aatcgctaaa ggttcttgca
     4621 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
     4681 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
     4741 tgcttataat gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
     4801 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
     4861 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
     4921 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
     4981 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
     5041 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
     5101 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
     5161 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
     5221 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
     5281 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
     5341 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
     5401 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
     5461 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
     5521 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
     5581 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
     5641 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
     5701 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
     5761 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
     5821 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
     5881 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
     5941 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
     6001 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
     6061 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
     6121 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
     6181 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
     6241 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
     6301 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
     6361 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
     6421 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
     6481 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
     6541 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
     6601 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
     6661 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
     6721 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
     6781 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
     6841 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
     6901 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
     6961 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
     7021 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
     7081 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
     7141 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
     7201 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
     7261 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
     7321 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
     7381 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
     7441 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
     7501 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
     7561 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
     7621 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
     7681 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
     7741 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
     7801 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
     7861 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
     7921 ggctcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
     7981 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
     8041 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
     8101 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
     8161 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
     8221 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
     8281 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
     8341 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
     8401 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
     8461 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
     8521 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
     8581 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
     8641 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
     8701 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
     8761 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
     8821 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
     8881 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
     8941 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
     9001 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
     9061 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
     9121 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
     9181 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
     9241 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
     9301 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
     9361 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
     9421 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
     9481 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
     9541 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
     9601 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
     9661 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
     9721 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
     9781 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
     9841 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
     9901 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
     9961 caaaattata agttggcata ccatcgcgtt tttgtatgac aaagtcgcca ccgatattat
    10021 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    10081 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    10141 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    10201 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    10261 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    10321 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    10381 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    10441 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    10501 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    10561 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    10621 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    10681 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    10741 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    10801 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    10861 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    10921 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    10981 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    11041 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    11101 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    11161 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    11221 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    11281 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    11341 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    11401 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    11461 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    11521 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    11581 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    11641 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    11701 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    11761 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    11821 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    11881 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    11941 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    12001 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    12061 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    12121 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    12181 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    12241 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    12301 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    12361 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    12421 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    12481 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    12541 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    12601 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    12661 atcccatttg ttttagcgat tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    12721 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    12781 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    12841 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    12901 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    12961 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    13021 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    13081 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    13141 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    13201 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    13261 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    13321 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    13381 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    13441 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    13501 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    13561 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    13621 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    13681 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    13741 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    13801 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    13861 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    13921 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    13981 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    14041 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    14101 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    14161 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    14221 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    14281 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    14341 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    14401 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    14461 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    14521 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    14581 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    14641 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    14701 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    14761 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    14821 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    14881 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    14941 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    15001 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    15061 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    15121 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    15181 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    15241 cgtgccacta ggcgtgaccg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    15301 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    15361 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    15421 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    15481 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    15541 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    15601 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    15661 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    15721 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    15781 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    15841 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    15901 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    15961 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    16021 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    16081 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    16141 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    16201 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    16261 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    16321 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    16381 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    16441 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    16501 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    16561 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    16621 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    16681 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    16741 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    16801 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    16861 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    16921 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    16981 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    17041 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    17101 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    17161 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    17221 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaaa
    17281 agcttgcatc tgcggtaata agcccaacca aacgaaaaat atgagaagct atttttgttt
    17341 ggctgttctt atgacttgat gcaggacagc tttttcacaa cctctgattt cttattcttc
    17401 ttcatttgta gcaaaataat tacggtgata aatgatacaa catacactga taagaacaat
    17461 ggcacctgca aaataaaacg catagctttc accaatatgt tcaccaatca cacctgcaat
    17521 aaatggtgct aaagctccac cgaaaaagcg gacaaaacta tagctggacg aagcaacgtt
    17581 tcgttgcaga cctggtattt ccatagcaat cgtcgtcaac agtgtattga taattccttg
    17641 gaaaaatcct gctaacacaa tgctgatcgg tacaaggact ggcatagaag ttccagctcc
    17701 taataacagc aaacaaataa tgaataagac aaatgctgct aaaattgtcc gataagtcgt
    17761 aaacttccgc tcaagagccg gtgcaacgaa aatcgaagca atagccagaa gcacacccca
    17821 gccaaaaaat acaaaaccga cttctaattc agaatagcca atcaataaaa atggcgcata
    17881 cgcaagtaaa gtaaagaatc cgaaattata taacaatgcg attagtccaa ctgaacgaag
    17941 tttatgattg cttaacgctt tgacaccttc ccaaaggcct acttttctct ttggtttctc
    18001 agtttcaggt aaaagtaccg ctagggctat aaaaccgcaa aacatcaaaa atgctacccc
    18061 aaaaaatgga taacgccatg aaccactgcc caagacccca ccgactagag ggccaacagc
    18121 cattccgcag ccaagtgctg cttcatacat catgattgct ttttctgtgt taccaactaa
    18181 aatactgacg atcgttgcta aagcagtaga gacaaataaa gcattcccga caccccagcc
    18241 ggcacgtaac ccaatcagca ttccgatatt cccagataat ccgccaagac cagcgaatac
    18301 aacgatgata aacaatcctg tcatcaatgt tttttcaaac cgatccgact agataagaaa
    18361 cctgtaaata gcatcaccac accagtgact aacatgtaac tagtaaataa cagggtcgtt
    18421 tcagctggcg tagcgtttaa ctgtgcagca atcgttttta agattgggtc tactagaccc
    18481 actcccataa aagcgatcat acaggtagct gctacaacta gcgctgaact tttccccttt
    18541 gtttcttcat ttgtttgttc ttgtatatct atttgattct ccaatatttc catccctttc
    18601 tctctattag catcatacat gatttatttt acatgtaaat atgcacatgt aaaacgtgga
    18661 aactttactg tgatataata aagaaaataa gacgagaggt aggattttta tgaatacttt
    18721 agcctatata ttattatgcg tgcttttatc caaaccacgt tctgggtatg aattgaagca
    18781 gctgatcaca attttttggg aagcccatca cagtcagatc tatacaacgc ttgccaagtt
    18841 agaccagcag ggttatgtga ccgttttgga caaagacgag cactctcaaa aaaagatcta
    18901 ccacttgact gatgaaggac gtgctcttgt agaggaatgg atcaaagagg aaactcccgc
    18961 tcctacgcaa aaagatgaat ttctagcaaa aatctatgct ttttcgacgc tcgataaaac
    19021 aacagctact ggactgctat tcactagaga acaacagctg aacaaaatca tgcaaaaaaa
    19081 tcaaaaaaaa ttgaatgacc tctcgctaga caagaaacaa gaatttggac gttatgttgt
    19141 gatccagcgc agaattatgc tttgtcagca ggaactccaa tggtgtcagc aagtaagaga
    19201 acaaatgcag cagttttttg agtaagggat aaacagaacc tttctagaaa tatctattga
    19261 agagcaaatt atcttcaata aaagagcaat acagttggaa aagaaaaagc gatccgaacg
    19321 acaggccaaa acctatcgtt cggatcacta tttttctaga actgcattca agagactatg
    19381 acaaaaagca tgccaaggcc ttttttcaaa taacagtgtt ccatcattca ttactttgaa
    19441 ttgcttttag cacacttctt gaaagcgggc caacgatgat caactgcaac ggtaaagcgg
    19501 caatcacatt cattttccaa gcattcatat aagcagagaa taattgatca ggtattcctt
    19561 gttccatgat cacaccgaat aaagacatac atgtcaccat tcctaaaacc atcaagcatg
    19621 aaatagttaa gatcatttgg accttgcttt ctggatttac tggtagttta aaggcaattt
    19681 ttttcgccag cacaccgatg atgaagacat ccaaaatgaa agccaccacg aatccaggca
    19741 ctagaccaat caacaaactt gataaatgaa gatcttgatg cagccataaa ttatacgcgc
    19801 tcatccccaa gaccatcaaa aaacacatca ttgttgtaaa aattattcct tcttttttat
    19861 ttgtaggcaa tctgtaccac tcctttaaat ttgtcacgaa aatcatcgta gcacatttaa
    19921 aaatttctcg taagtacttt ttttattttc tgccctttta tttcataaac ttcatcagcc
    19981 gtacgctcga caaagtaaga atcatgagaa gtaaacaaaa tcgttcccgg atatatttgc
    20041 attagcttct ctaaagcttc gatcgttgcc atatcaataa aattagtcgg ttcatctaac
    20101 agcaacacat tacttggctt agtaaaaagc aaagcaatcg tcagacgggt cgcttctccc
    20161 ccacttaatg tacataacgg tttcgtcaaa gtttcctgag caaaccctaa gtgattcagc
    20221 aaactacgga caagcgattc tgaaaactct gtttgtctca tgacaaaacg aatgatggat
    20281 tcttcagatg tcatttgata acccttttgc tgatatactt gaaagcttac tttcggagag
    20341 agaaggattc cttctccttg tttgcggata tgttctaata aggttgtctt acctgaacca
    20401 ttttcaccga caatcgcgat ttttttacct aaagaaaact gaaaatcacc atttaccagt
    20461 aatgttctcc cacttctttc caattggaca ttttgtccca tgatgggata gcggctgtgg
    20521 agttcaagag attttggtac tggaaaacgg atttgtttca tctgctgcgg cttggttgtt
    20581 tctcctattt tttggagtct tctctctaat gttttcgcat tcttttggat ggctttttgt
    20641 acactgtctt tttgtttaga ggaagacaac cgatccggtc tgatttgttg ctttttcttt
    20701 ttactggaca ctttttccaa cttttccgct tgttttttct tttcttggat cgatttttcc
    20761 aatcgttttt tctctttcat gatcgactct gcttctcgta gctgagtctt tttctccaat
    20821 tccttttgag tgagataggc actataattc cccgaaaact ctcggatttc tccgtctttt
    20881 acttcccaga ttttcgatgc tagctcatct agaaaatagc gatcatgact aacaaaaacc
    20941 agcgtaccat aataataacg aagctcttca atcagtcgat caatactttt tctatctaga
    21001 tgggttgttg gctcgtctag aagcatccct tgttcataag aagaaagaag acgtatcaac
    21061 tcgatctttc gttcttcccc tccgctccat ttccgctctg aagtttcttc cagtcttaac
    21121 tggaaatgac tggctaattc caaatttagg tcctttgctt cagcgagata ggttaattga
    21181 ggcaggtagt tgaaggtgat ctctgtttgg attttcccat gatcgggaaa aagttcaccg
    21241 gctattagtt tgagcaaagt cgatttaccg acaccatttt ctcctatgat ccctatacga
    21301 tcttgctgat agacacgaag agaatcaata gatagttcta gctggttgcc aaagctgact
    21361 tgtagatttg ttatatttac tgctaaattt tccatatgtt ttacactcct atttcatgta
    21421 aaacaacaat tatcgatact aaaaaagagc agaaaaatct gtttgcaaaa aaactgcatc
    21481 acatcttttc ctactctgaa cgaaataact atgactaaat caccaaatct gtcatagctt
    21541 tcacctattc tactgtcgtt cgaaaaaatg gacatactcc atccatgaaa ttgttgttta
    21601 tacatgtctt ttgaatttat ttagttgtta ttcaaaagtt cgaaacgtaa tttcatcgat
    21661 gcagtcattt ttccgacctc cttttttatt aggttaccac acacgattca aaaaaagcaa
    21721 tagctactga atagtaactg aaaacctttt attcgaacgt agtgatcttt tctggtgcta
    21781 aacgaacaga ctcgtaacct tcttctaccg cttttcctat tgataaaata ataactggaa
    21841 cataacggtc tttatccaaa ccaaatgcct ctgccaactg atctgcttcg aatccaccaa
    21901 ttggatttgt ttcatagcca tgatcacgcg caactaacat gaattgcata gccgctaagc
    21961 tagaatcgac tttgactaca tcgttcattt cttctcttga aaaactcttg taataaggaa
    22021 tgatcgcagc taattgttga tctctgactt cttttggcat cttccctgat tcgtaagctt
    22081 ggttatagat ttcttcgcca tattcgtaac attgcatatc gccaaaaatc atgaccattg
    22141 cagaagaagt atcattttgt ctcgtattga aacggatcaa aggacgcaag atttcttttg
    22201 cttcctcgct ttcagcgacc acaaagcgcc atggttgcat attgacagaa gaaggtgcag
    22261 tcgtcgcttc tttgatcatt tccagcattt cttcgtgtgg gattttgtat gtttcatcat
    22321 atacgcgaac ggatcttcta ccagtcataa ttgttgaaaa atcatttttt tgttccatta
    22381 ttattaacct ctttcgtttc tgttgttata ttgcaaagtt gggtattcaa tttatctaaa
    22441 agtgttagaa attgtttcat ttctacatcg ctgaaagccg gggatagcac ggaacctgta
    22501 tctttatggt ccatttcaca atgctcgatt ttcttctggg ctttttctgt tagcgctaca
    22561 tacacttccc ggttgttctg aggatttctc tgacgcgaaa catagccatt tgcttccaat
    22621 accttcagat gacgagtaac tgctccttga tcaatctgca acttttcttg caaactgctt
    22681 tgtagcaatg gattttcttc tttcagtctc atcatgatct cgtatctagt aagacttata
    22741 ccgatatttt cttcaaagat ctggacgatt ttttgatcgg ctaatttgat ttgatataaa
    22801 aggtgactaa tctcttctaa agacataatt tcctcctttc taataattga ctcgtcaacg
    22861 attgacttgt caataataga ataaaaaaag aaaaaagtca aagaatcagc tgtgtaataa
    22921 aaaaaacgct ctcaatagaa aagtatagag aaaagaaatt ttttgtagaa tgaacggaaa
    22981 aaatgataaa gaaagcacaa cgctaaaacc aagattattg attcttatca aactatagaa
    23041 gatagctctt ctgcagtaga aaattttaca ccgccaaaaa tttccgaaat cgccttttgc
    23101 aagaatcccg tcgaatatta gaaattggat cagtatgctt aaacagtaat aaaaaagtta
    23161 tgacaagatg ttgtcacaac cttttttgag taaacggtgt tcaaaaactt gcatctgcgg
    23221 taataagttc aaccaaacaa aaaacatgaa aatctgtttt tgtttggctg ttcttactac
    23281 ttgatgcagg gcagcttttt cactacctct ttttaataac cataaaatcc tattccttca
    23341 tatctccagc ccatcggtgt tagccaatct ctttccttag cactcaaggt atagtgatgg
    23401 gtaccagttt tttcatttgg attaaatagt cgataaattg gcttggaacc accagaataa
    23461 aaacttagac cttcataacg ccaaccgatt tttgttaaag agttgatctc agattgtgcc
    23521 attgtgtaat gatggtctcc actgtaagga ttgtataaac ggtagacagg ttgtccgctg
    23581 tttggtgctt gccaagcaat cccttcatat tcccagccta cagttggcag ataatttttt
    23641 tctccggcat tcaatgtata taaatgttct cctgtatgac ggttatacag acgatacatc
    23701 ggagaagttg aagttggtgc tgtatcatac tgtgtcagat tataggtttc gattacattg
    23761 ttcaatgtag aaccataatt aggcgcagtg gcataacgac ctgttagcca agccgtagca
    23821 tctttgtaag agttcgtatt gcttttccat gcaccggaat agtagtaaac gcctggctga
    23881 aaagaagtcg tcttcaatac cgttacgtta tcttgcaatg attctttgta cgaaggatat
    23941 tttctaaaag cggcattcac cgtaatccat tgcccattca catattccga agtaggcatc
    24001 gtaacagatt ggccattata gcttccttta atcccaaata gattgtaatt aggggcagaa
    24061 gccaaagtac tgttccccca gccactttca acgatggctt gagcaatcat gactgatgca
    24121 tataagtcat tggcacttgc caaactttgt gcagttgagg caatactatc aatgaacgct
    24181 tgttgagaat tagcagatga gcgagcataa aaagcatagc cagtatagtt ttcgaattca
    24241 gacaaatctg cttgatgggt atgtgtcaat gtttgcccat ctgtcaattg gttcgtgatt
    24301 tcttctgcat tctctggatt gttctgagct tctgctgatg tactgtcttc ggtttgtgtt
    24361 tgttctgttg tttcagttgt tggttcttcg gttgaaattg tattttctga gacagtagct
    24421 gactgcaaag tactctctgt atcgttttca attcctgttt ctgaagaact cgatgtctga
    24481 gttgcgacag tttctgtcga ttcttcagct agcactgttg tgttggcaac tgctagattt
    24541 cctaaaagaa taacgctaaa taataaattc tttgctttca tcttttttct cctttgaatt
    24601 atgaaacttt tcctaaataa cagtgtaact aaaaaagtaa aagcaagtca atgtccatat
    24661 ttaaataaca tttgtttctt tttgataaat ttgctttttc tacataaaaa gacgtaaatg
    24721 aagaatattc ttctccattc acgccttaaa ttgatggtcg tttgaataac tatttaacaa
    24781 gtaattctct ttggatatta tccgttatgg attatacttc ctgtgtcgtc ggacggataa
    24841 tcatgttact gacagacatc cggtctggcg tttctatcgc aaatgctaca gcagaagcta
    24901 tatcttcgga ggtcaatccc cattcttttt gcgcttcatg aagttcaaga gcagcctttt
    24961 tgtcgctgat ggtttgatat aattcagttt gaacagctcc tggagagatg atcgtagatt
    25021 tgatgttatt ttgccgttgc tcttgacgta atccttccat gattgcacgg acagcaaatt
    25081 ttgttccgca ataaacggcg gaatcaggat aaacaacatg tcccgccaca gaatcagttg
    25141 aaatgatatg cccagatttt tgtttttcca tgattggaag tacggcggaa attccattta
    25201 acacacccat gatgttgata tcaagcatat tttgccattc atcacggtga ccttcaacta
    25261 atggagcggt tggcatgatt cctgcattat tgtacaatac atcgattcga ccgaactttt
    25321 ccatcgctaa atcgataact gcttggactt gtgcaaaatc tcttacatct gctgtttgaa
    25381 tatatagctc agcttctggt aaagattctt ttattgcttt taggcgatct tctcggcgtg
    25441 cagctataac tagtttagcc cctttttctg ctaatagctt cgttgttgct tcaccgattc
    25501 cgcttgaagc gcccatgatg acaatcactt tttctgataa atcattcatt ctattctcct
    25561 cctgttttga aaaatctctt gcttatactc actatctctt atttcatctt actgttcaaa
    25621 taatccgttc gacaggtttg acgattttgg cagggattcc tgcaacgatg gtattgtctg
    25681 ggacatcttt cgtaacagtt gcattcgctg caacaatggc attctcgcca atcgttacgc
    25741 ctggcagaat agttgctcct gctccgatcc aagcattttt cttgatgtgg attggcttta
    25801 ctgacaagcc tcttcgttct tttggatcgg ttagatggtt caccgtgatg aggttgacac
    25861 gaggaccaat caatactgaa tcttctaaac agatgccacc taagtcaacg aacatcactt
    25921 gacgattgat aaagacattt ttccctatac taatatgttt gccgaaatct gtataaaaag
    25981 gcagcagaac agtcaccgta tcatctactt tgtcactaat gatattttct agccgtttgc
    26041 ggatatctgc tggttcatgg tacccagtat tcagcgctgc taaaggctct tcgttttcag
    26101 cttggatctg atggatcact tcaaagagcg gatccgttac tcgaatctct ttattgacga
    26161 tttgtttttc gatgaggctt gtcattttgc tccctccttt tatccttact ttataacgga
    26221 cggcttatca tgtctaatac ttataaaaca tacttaacta ttccttttaa ctatacttag
    26281 tggtatgctt ttttaaaaac aggagggaaa aaatggatat tcgtatactg acgtattttt
    26341 tagcagtagc tagagaaaaa aatatcagcc gagcagcaaa atctctgcat atcacgcaac
    26401 ctactctctc aaaacaactg aaagatttag aagaagaact tggtgtgaca cttttcactc
    26461 ggggctcccg agagatacag ttgacggatt ctggtcgata tctttattca aaaggaaaag
    26521 aaatcttgtc gttggtagaa aaaacggcga acaatttaac aaatgacgca atcatctctg
    26581 gagaaatcta cattggcgga ggcgaaacta aagcaatctc tttcatttct gatagccttc
    26641 attcgttgat caaggcgcat cctgatatcc agtttcattt atatagcggg aatgcagatg
    26701 atatcataga caaactggat agcggattac ttgattttgg actcgtaatc gaaccgacaa
    26761 acaaacaaaa ataccagtca tttcgactgc cagcccaaga tcgttgggga cttttgatac
    26821 atcgaaatca tcctttagca aagcaaaccg tcatccatcc gcaggaactg aaaactgttc
    26881 ctttgatcat ttcccggcaa acgtcagtgg acagtcaact agctgaatgg ctaggtttca
    26941 gcatagatca gttatcgatc gtcggaacat ataacttatt gtacaatgct tctttgatgg
    27001 tcgataacgg gacttttggt gcattagctc tagatgggat catcaatact tatgggacgg
    27061 aaaaaatatt catcccactc gatccgccat tacaagcttc actgaatctc atttggaaaa
    27121 aagaccagcc tctttctcct gctgctcgta aatttttaga tgtcctaacg gagaaataca
    27181 gttaagtaga gtttttttcc ttcgctttca gctttttaac aacgaataag ataagcaaaa
    27241 gatccaacga ttaaataaca taagtttctt aatttatttc ttccttttat ttctgataat
    27301 tccagtaaat tactataatt ataaaaaaag aattgacaaa acataactgt ctatataaaa
    27361 ttgattacgt taacaataat tcgtttcatg tgactagatc atactaggca tgaaaaatct
    27421 ctgaggaaca tggtttccct aggaggtttt tcgtgtctat ttttgtttgg ccgcaatcaa
    27481 ctggtctagc aaacgtgaaa accaaatctt caaaaagggt gcgcgcatga aaatcaaaaa
    27541 gatctttaat caaaatgcag tattggtaga tgacggtgga caggaaaaag tagctattgg
    27601 aaaagggatt ggatttgaca gaaaaagaaa tgatttgatt tttgatcgtg atgtcgaacg
    27661 gatatttgtc atggaaccag aaggtcaaat caagttgcaa aatttactta atcagattga
    27721 tgaacgcttt ttatttgcag cagaaacgat cattgatcat gcggaaactg ttttgatgga
    27781 aaagttgaat gaacatgtat tgatcgcttt gaccgaccac attgcttttt ctgcggagaa
    27841 cattaataac ggtatcgtca tccgcaacaa acttttaaga gaaatcgaag tgctttacag
    27901 tgatgaattc agtattgctc aatgggcagt agagtatctg acaaaagagc ttgggattcc
    27961 atatacttat gacgaagcag gatatatcgc aatccatatt cacagcggac gaagcggtcg
    28021 gaacaataac catcgcagta tccgtgaagt gacgattgta tctgacatca tccatttagt
    28081 ggaatcagaa ttggaaacag atatccattc agaaccattt tcactgaatt attcaagact
    28141 ggtgaatcat ttacgactgc ttctacaacg aacccatgcg caacaatatg ctgttttaga
    28201 cacggaaatc gtagaaatgg tcaaacgaaa atacccagaa agctataaga tttctaaaaa
    28261 aatccgtgtg ttgctgacca aagaatacca gctcgcgatc acgaaagaag aactgggtta
    28321 cttggccatc catatcgagc gtttaagaag cgcaatcgta caaacaaatg ttaacaatca
    28381 tgaggaggaa aaaaattgaa agcatatcta caacgaatgg gacgttccct tcaacttcca
    28441 gttgcggtct tacctgctgc tgctttacta gtagggatcg gaaactggtg ggcatcttat
    28501 agtaacgaca ttgtcgctca ttttttacaa gctggcggta acgcagtttt aggtcaatta
    28561 cctttgctgt ttgccgttgg gttagcacta gggatgtcag tggacaaatc aggtgctgct
    28621 gccttggcag gtttggtcgc atttgaagtt ccagtaaatg ttttaaaaac tgattcagta
    28681 gctactttat tgaatatcaa accagaatca gttgatccat cttttgctca gatcagtaac
    28741 gtatttattg gtattattgc cggattgatt gcggcagctc tttataatcg tttccacgaa
    28801 gcaaaattac cgatggcttt atctttcttt agcggcaagc gtctagtgcc aatcttggct
    28861 gcctttgtca tgttgctgat cagtgcagtg ctactgctcg tatggcctcc ggtttactct
    28921 gctttagttt cttttgctaa attcattttg agtttaggct ggggtggtgc aggtctttat
    28981 ggcttcttca accgtttact gatccctaca ggattgcatc aagcaatgaa ctccgtgttc
    29041 tggtttgacg tagcaggaat caatgatatt ggtaaatttt tggcttctga aggaacaaaa
    29101 ggcgtaacag gaatgtatca agctggattt ttcccaatca tgatgttcgg gcttccagca
    29161 ggagcatatg ctatttacag aaatgcacgt ccagaaaaca aagcaaaaac agcttccttg
    29221 atgctagcag cagcttttgc ttcattcttt actggtgtga cagaaccact tgaattttca
    29281 tttatgttcg ttgcttggcc attatatgtc ttgcatgcaa ttttcactgg gatttcttta
    29341 gcagtgagtg cattcttcca ttggacagca ggatttgcct ttagtgccgg atttgtcgat
    29401 tacttcctat ctttgaaaaa tccgattgcg aatcagccat tgatgttgat cgtccaagga
    29461 ttagtaacag cagtgattta ctactttggc tttaactttg ctatcaaaaa attccatctg
    29521 atgactcctg gccgcgagga agcagatctt tcagatgacg atactgcaac gactaattca
    29581 gacaataaat atgcggcaca agcaagcaaa atctacgctg cattaggcgg agccgacaat
    29641 gtaacttcga tcgataactg tactacacgc cttcgtttgc aagtcaaaga tacaggcctg
    29701 atcgatcaaa ataaaatcaa agccactggc gtacctggta tgaagatcat cgacggaaaa
    29761 aatgcgcaag tcatcgtagg tacagaagta caattcgttg cagatgagat ggcaaaacta
    29821 catggtggtg ctgcagcacg acctgcacaa acaaacaccg tggttaaaac agaaacattt
    29881 gccggtgaaa cgatcgaaca agatatttac gctatcgcaa atgggaaact gattccaatc
    29941 actgaagttt cagatgatgt attcgcggaa aaaatgatgg gtgacggcta tgctgtatta
    30001 ccagaaaatg gagaaatctt ctctccaatc gctggtacga tcacaaacat tttcccaacg
    30061 aaacatgctg tcggaatcca aacagatgct ggtatcgaag tcttgcttca tatgggcatc
    30121 aacactgttg acttaaaagg cgaaccattc actttatatg tagaagaagg tcaaaaagtc
    30181 gcaagaggtc aattgattgc tttagttgat cttgccgcta ttcaatctgc aggcaaaaac
    30241 acggatatgg tcgttgtatt cacaaatggc gacaaagtcc aaagcttaga aatcgaaccc
    30301 gcacgtgatg taaaagcaaa tgataaaatc ggctctgttt ccaacaaagc ctgagccatc
    30361 gtcttcccca gactttttta tcgataaaca aacaggagct gagacaaaaa tcttgtctca
    30421 gctccttttt ccgaataaaa acggcgttct cctgccaact ccgcggtaat aagtccagct
    30481 aaacgaaaca tatgaagagc tattttttat ttgactcttc ttattacttg atgcagaaca
    30541 gctttttcac aaaatttttc ttcatactca aaactaatcg gctttttcac aacctcttta
    30601 tacattattc ctgatccaat atttctttta atgctgcttc aagagaagtt aatggttttc
    30661 ccaatacttt ttctaaatca tcagaggaaa tagctaactg atccttttta atatcatgtt
    30721 ggatcgctaa aaacatgtca gctgcttctt gagggaatcc ggcttctttt agattttcga
    30781 caaacgctgc atcacttgct tcaataattt ctaactcttt acctgtcgca cgctctaatg
    30841 ccttggctaa ttcttcatag tcagttagat tgccggataa ttctaagatt tctggagagt
    30901 cactttctac tagtactttt gctgcagctt ctgcatattc acgtttcaat gcccaacctg
    30961 tttttccatt tcctgcagca taaacgaatt gaccagtttt taaagcctgc ccaatgatag
    31021 gtaattcatt ctctacatac caattattcc gtaaaaatgt atgggaaata cctgaatcct
    31081 taatcatttt ttcagtgaat tggtgatctg gcgctaatac actgatcgag tgatcggcat
    31141 ctgcaaaact tgtataagcg atataagata ccccagctgc ttgtgctgcc ttcactacat
    31201 tcttatgttc ggcttgacga tttcctggaa cgccagagac aaaaagcaat cgatctattc
    31261 ctttcaacgc ttctttcatt gagtctaaat catcataatc accaatacgt atattaaatc
    31321 cagcttcttt cagtgcttct cctttttctt cactacgtac tagtacatag atttcatttt
    31381 gtggaaccat cttttttaag aattccagcg cataaccacc aaaacctcca gttgctcctg
    31441 ttactaaata atgcatattt gccactccct attcttttta tttgttaaga tttacagctg
    31501 tacatttata aattacacct ttatcataaa gagtgtggta agataagtca agcaaaaaaa
    31561 atgaaaggat gacaactgat gaagtattct ctgcaattta gtgatgcaat ccatgtactt
    31621 gcctatattg aaatgtttca agatacccga tcactttcta gtgagatgat tgccagtagt
    31681 atcgaaacaa atgcagcaaa tgtacgtaaa atcatgagtc agctcaaaaa atccggtttg
    31741 atctttacaa aaaccggaaa accgcagcct tcccttgcca aaagtccaga tcagatttct
    31801 ttactagatg tctatcgcag catcgaaggt aatacacagc ttatccaagt cgatccaaaa
    31861 acaaatccaa attgcgtcgt tggtgcgaat atccaagaag ctttagaaga aagctacgct
    31921 cggctgcaga aaaaggcaga agaagaaatg gctaagatca cacttgatag tctgatacat
    31981 aaaatagccg ttttggaaaa agaaaaaaga ccagaaaaca caggcctcgt agagagattt
    32041 ttataattct ctctactgtg cctgcatttt tctgatcgga atcgtctaca taaattcttt
    32101 tattctccgg gggacaaaag ccttgtcatg cccctttttc ctctctaatg ctcaggtcaa
    32161 agcgctgaag tcatgacgtc ttgataaacg gtgttcaagt agctgaccct cggcattagt
    32221 tctgaaaaat gcaaaatatg gaaagctatt tcccattttt cctctctaat gctcaggtca
    32281 aagcgctact ttcacgacct ctatattatc atggtttatt gactaccggt atccagattt
    32341 ctcccatata tttcccgttg gtaactccca tttcgactac agcatttgga cccccaacat
    32401 aggcgtaatc ttctacttct cttaaaacat gcccaaaggc cgtattcgtc aaatcatttc
    32461 ttaattcttc aggcgattct gctaaagcgc ttacaacaag atacgttcct tttgggaatg
    32521 ggatgattcg cgtagtgttt ggaacatcct gactcgtttg aacaccgaca tagtacatca
    32581 ttttcccgtt atagggttcg ttgactgtaa agaaataatg attttcagca gcttgtttca
    32641 atgcatctat tgttccatta ccgataactt tttgctcaaa tgcttgtttc tctgcagcca
    32701 aaccgacaaa atccgtattg ctactcttca attccacacc aatacccgtc accgtaaaat
    32761 ctggtttttc ttcgatccta tactttgcca tttctcccac cctttctatt atattaagtt
    32821 catcttacat aaaattaaag ggaaatgaca cgattacgct cttttttcta gggatatttt
    32881 tattttttaa ctattttttt gctttcactg caatccaaat ctcactgtaa acattactga
    32941 aatcctctcc ccattttgta aaagagatct cgggagcttc gactaaccca taatctgaag
    33001 aaggaagcca ttctgcatag attttccccc aagtatcttg taaagtttgg ggaaatggcc
    33061 ctttattagg aaaaatggcc caagtgtgtt cttctatagc aagcatttct aaatcagcgt
    33121 acggattttc ttttgtcgta gcaaagccaa tcatgtgcat caaactgccc ttttcttcca
    33181 agcggtctcc atcaaatcca taagaaacgt tgattacttg atttggatat aaatctccta
    33241 acttatgcat ctcttccctt tgagtctccg tgatcgtctg cgctaacttt tgtatttctt
    33301 gattgactcc ttcaaatttg acaggaactt ttttcgccac acctgccaca taaaactgtt
    33361 ctttcttttc gatacgaaat tccatcgatt ttcctcctct gacatcaata aaaaatgtca
    33421 agcgtggaaa agatttttgt atgccgtatc tactgatttc tgaaggcaga tatcctgacc
    33481 aatcacgaaa agctcttgaa aaaccttcga ctgtttcata tccatatcta aacgccgttt
    33541 ctgtcactct tgatccatct agaagatcgc gattagccaa agccaatcta cggtttttga
    33601 tatagtccat tagcgacatc cctgcaataa acgtaaagat gcgttttaat tgatattcag
    33661 agattcctgt attttcggct attattttat aggaaagtcc ttctgtcaaa tgttcttcga
    33721 tgtaatcgat caattggttc agatctttaa gcatatttat cttcctcctt gattgccttt
    33781 ttatcatatt atcaaagaaa gtcaattact tgatgaatca ggaaccaatt tagtcagatg
    33841 tagaaaatgt tgtatggatc taaagaaaat tctcaagcag cgtttcgtaa cggataattg
    33901 gtattatctg cctatctatg ttttaccttt agaaaaataa attttgggga agacagattc
    33961 taagtaaaca aagcggtaaa aatatctttt attcccttag ttaatccgct tactacctac
    34021 tgtacaaagc aaattgttgt tcaaactgct gatcagaacg ctgcttggat ttttctttgc
    34081 gatacatcat acctctttta tcaattggaa caattattta tttctaacaa aatcacttca
    34141 tttttgtcag ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta
    34201 ctttttctca cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa
    34261 agtgattatc aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat
    34321 ataaataagt gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga
    34381 aaacgttttg gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta
    34441 tacaagcaag tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac
    34501 ttgggatgaa aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg
    34561 caaacacatg gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta
    34621 ctcactttcc gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct
    34681 tttaacagag attcttatag aacatccgca ctttatcgaa cgcttcaaag ttaagatgcc
    34741 atttcatgca ataaattatt cagataaatt aacaactatc atccataaat acgatcatat
    34801 tatcaagaaa aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat
    34861 ccaagaagca gttgaatcgc ttattcagca aaatgcctat cttcccgcat tcgaattgat
    34921 caatgaagtg attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct
    34981 tcttctgata gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc
    35041 agaaaaaagg caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta
    35101 ttcaaaacgc tactctataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa
    35161 ccaaaagaaa aaaatagata aaaaactaaa gaaacggtaa agaaagacga tttcaatgaa
    35221 aaatagcaag taaacgataa caaccatcct aaatccacaa gaaatacagc agaccttgct
    35281 ttaaaaacac gaatcatgca aaggatgaag taaagaagac ttaacgcgta cccaatatta
    35341 gtcatttgac tattaggtac gcgttttgtt ttataagtca tgacaaattt ccttcgttgc
    35401 tggatagatt ttccggcatt gttgatagat tttatgatac ttttctacat tttgtaagag
    35461 aggttccgct gcatctgtat attggataaa attctgacaa caactttcag gtgtgtcgaa
    35521 ccaccctaac cctagtgcag caatcattgt tgcgcctaat gcaggtcctt cctccatact
    35581 taaacaatag accgttgcat cgaaaatatc agcttggatt tgtagccatg caagattttt
    35641 cgcgcctcct cccaccgaaa caatacgtgt aaatctgcga cccgctagtt ctttacgttt
    35701 caatcccacc attgacaaat gctcattaaa aatttagaat attcacaagt ttaacgacaa
    35761 ttatgaaaat ttatgtcgtg ctaaaggagt tttgttattg gatgaaaagt tagatataaa
    35821 aactgcgatc aaagacacgt tacctaccgt tttcggttat atcggtattg gacttgcatt
    35881 tggtatcgtt gggaaagctg ccggatttca tccactagtc gtcacgttga tgtccctgct
    35941 ggtctatgct ggttctgccc aatttatcac agtcagcatg cttgctagtc acagcccatt
    36001 gctttccatc gttttctcga cctttctagt caattcccga atgattctga tgagtatgac
    36061 gattgctcct tatttcaaga aaaatcggct gcttcaaaat ctgctgattg gcacattgct
    36121 gacagatgaa agtttcgcac tgggaatgaa taaattgaac tatactggac aaaagttaaa
    36181 ctttcgttgg atgaatacag ctaattggat ttcttatttg acgtgggtgg gttcttctct
    36241 tgttggcgca cttttaggga attttattac tgatccgaaa aagtttggtt tggatttcgc
    36301 catcgtcgct atgtttatcg ggttacttta tcttcaagtc atttctgata gaaacacctc
    36361 taaacgatta cagttgatcc tgattggtct cactttgatt ctggtatatg tcggactgat
    36421 ttttatccca agcaacttag tgattgtagt tgtaacactg attgggtgcg gattgggggt
    36481 gtggatcaaa catgccttct tttgagttta cattgctgac tattttaggt tgtactttag
    36541 caacttggct ttctagggtc ctaccatttg tcttactgaa aaaattcgat ttaccgcaac
    36601 cgttgcttga gtatttaagt tttgtcccga tcgtcattat gtctgcttta tggttcagca
    36661 gtctatttac ccaaaatatc ggacatctgc cgcagatcaa tctcgaaaat gctttggctt
    36721 cagttcctac acttttagct gctattcttt caaaaagctt attggtgatc gtcttggcag
    36781 gaattctttc cctttcgttg attcgcttat tataaataaa aaaacaagag cttatggcaa
    36841 attactgtca tgcgctcttg tttttatcaa agtcaaataa gaaaaaaatc ggcgtattct
    36901 tgcttatccg ttgaacggac cactaacaac gacttttcct aacatatgtc ctgtttctac
    36961 gtctttggtt gcttgtttca agttcttcgc attcatccca tctgaataga ccttgctcaa
    37021 tgtactgcgg ataatccctt catttgctaa agcagcaatg tgcgctaaag cttctccttg
    37081 cgtttcgatt tcatagttat agtctgtttt agcgaacata tactcccagt cgaagctgat
    37141 agattggttt ttccaaacac ctatatctaa cggctctttg atccctacga tcgttcccac
    37201 atgtccaaat ggtcggacaa ggtgcttgat ttgatcaaag tagcgggtaa tatcgaataa
    37261 tacagcgaca tactctactt gatcgattcc taattcacga aaacttttac ttaagtcgtg
    37321 atgataatcg attggatagt ctacacctgt ttttttcagc cattcaaaat tcttcggact
    37381 tgcagtcgcg tagacttcta ggccagccca atgcgctagt tgattcaaaa tcgaaccgac
    37441 gcccccggat ccgttgatca ctaagatttt tttcccgcga ttggcatttt cttctggagt
    37501 tagaccaaat ttttcaaata acaattcata agcggtcaat gaggtcaacg gcaaggctgc
    37561 agcttcttca tctgaaagat tttttggtgc aagagcgaca atacgttcgt caactagttg
    37621 gtattcttga ttgcttcctg ctcttgttgt tgtacctgcg taaaaaacgc gatcgccgac
    37681 agaaaatttt tgcacttcgt cacctacagc aacaactttc ccgactccat caaaacctaa
    37741 gatacgtaaa gcatttctta tcccgttatc ttggcgaagt ttcgtatcga caggattaac
    37801 agatactgcg ttgactttta ccagaagatc ccgtgaactg ggtacaggta tctgttcttt
    37861 ttcatcaata aagctttttg gatcatcgat cggcaaccct tcataaaaac cgactacctt
    37921 cattaattcc ttttctgaca tacgaattcc tccttagctt catcaagttg gagcatccat
    37981 acagtgttca tttcaatata tatagtatgc tatttgttcc acctttttta ttcatcataa
    38041 ttattctctt acttaaagta aactatttgc acttttttgg ttaaaaaaga ggttgcgaca
    38101 agacttttgt cacaacctct tataaacggt gttctcctac caactcttcg gtcacaagtc
    38161 aaaatattca tcaaacatga gaagctgttt tttgaaattg ctccttgtga ctcagagtta
    38221 aacggcaggc tcacaacctc tgatataaac ggtgttcaat caactgaccc tcggtattag
    38281 ctcaaaaaac gacaaaatat gaagagctat ttttgtttgg ctgttcttat tacttgatac
    38341 aggacagctt tttcacgacc tctaattcac ggtgttcaag caagaaatca ttgttcactg
    38401 aaaagcgttg gatgttcttt ttcaaaggtc tctaagatct ctcgtacttc atcagtagat
    38461 ttcgtattca tcaactgaac tcgcaagtca tttgctcctg gaaatccttt gatatagatt
    38521 ttaaagaaac gatgtagtcc tgtgatggaa cgcgggatga tttctgaata atgatcttgc
    38581 agatctagtt gcagacgaag tagtccgatc agttcttttt ctgtatgttc cctcgggtct
    38641 ttttcaaatg cataaggatt cttgaaaatc cctcggccaa tcatgatgcc atcaacgcca
    38701 tatttctcgg ccaattcttc tcctgtctgg cgatcgggga tatctccatt gatcgtgatt
    38761 agtgtttgtg gagcgatttg gtcacgtaaa gcaatgattt taggaatgac ttcccaatgt
    38821 gcatctactt tactcatctc tttacgcgta cgcaaatgga cagaaagatt ggctatatct
    38881 tgatgaagta ggtgactgat ccattcttcc atctcagaca tttccgtata gccgatacgc
    38941 gtcttgacgc tgacaggcaa accgccagct ttagctgctt caatcaattg tgccgctact
    39001 tctggacgaa ggatcaagcc actgcctttt ccacgttctg ctacattagg gactgggcag
    39061 cccatgttga tatcgattcc ttggaagccc atttctgcca tagcaatgct catttctcta
    39121 aaaaattctg gcttgtctcc ccaaatatgg gcaaccatcg gctgttcatc ttctgtaaat
    39181 gttaatcttc ctcgtacact gtcttttcca tctggatgac agaaactgtc tgagtttgta
    39241 aactcagtaa agaagacatc cggtgctgct gcatgcttta cgacatggcg aaatacaaca
    39301 tctgttacat cttccattgg tgctaaaaca aaaaaaggct ttggcagctc tttccaaaaa
    39361 ttagtagtca tttatttgtt actctccatt ctatcaagca gtgaagtcgc aatctttctc
    39421 aaaaacattg tcattatact aggaaacaaa aaaagaagca aagtgaacag cttaaaagat
    39481 gtttctgatt gagcaaaaaa aagactgtga caaaaatctt atcacagtcc attttccgga
    39541 ataaatggtg ttcaaaagct tgcatttgcg gtaataagtc caaccaaacg aaaaatagga
    39601 ggagctattt ttgtttggct gttcttatta cttgatgcag aacagctttt tcacaacctc
    39661 tccactaaac ggtgttcaat cagctgaccc tcggtattcg ctcaaaaaac aacaaaatat
    39721 gaggaactat tttcccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    39781 tcttttctaa cttctcaatc ctcttccttt attgatcaaa taccaagtaa cggcgtacaa
    39841 aacaacgatg aataacacaa gtacgatcat cgatgttgct actggcacat cgatcgttcc
    39901 aaggaatccg taacggaaac cggagatcat ataaacgatc ggattgactt ttgaaacagc
    39961 ttgccaaaag ggcggtaaca tagagatggc atagaaaacg ccacctagat aagttaacgg
    40021 ctgcaagaca aatgtaggca cgatcgatac atcatcataa gactgagcaa aaatcccgtt
    40081 gagcagtcct gctaatgaaa acaagattgc cgtcatcaat aatgtaatga tgaccatgaa
    40141 ccatgaatac acatgaagtg gtacaaagaa caaggaaatg atcgttacga gagagccgac
    40201 gagaatactg cgacccacac caccaatcac aaagccccaa ataatcacat gcgtaggaac
    40261 aggtgctacc aagatttctt cgatattttt ctgaaatttt tgagagaaga aggacgaaga
    40321 aacattggca tacgaactag tgatagcaga catcataatc aaaccaggaa cgatgaattc
    40381 catatatgaa aaaccaccca tgtcaccgat ccgcccaccg atcatttttc cgaaaataac
    40441 aaaatacaag gatgtcgtga tcactggcgg aaccagtgtt tggacccata tacgcaaata
    40501 gcggttcgtt tccttgatag ctaaactttt taatgcagta aaataaagat taaacatttt
    40561 tctcctccgt atgattattt tcttctgtga ttttcaagaa cagttcttct aaacgattcg
    40621 acttattcct catagaaaga actttgattc cttgtcttgt aagctgatcg aagattccgt
    40681 tcaccccttg atttcgttca acttcgacag cgattgtctg ctcatcttca aatgtacaag
    40741 catatccttc aatcacaggt ttggtatcat atggcgctaa atcaaagata aatgtttcaa
    40801 attgcagttt tgacagaagc tctttcatac ttgtattctc gatcagttca cccgattgga
    40861 tgattccgat gttgcgacaa agcatttcag cttcttctag ataatgcgtc gtcaaaatga
    40921 tcgttgtacc attttcgttc aattctcgta gaaactccca catctcgcgt ctcaactcga
    40981 tgtcaactcc tgctgttggt tcatccaaga tcagcaaacg tggttcatgc atcaatgccc
    41041 tagcaatcat cagtcttcgc ttcattcccc cggaaagcat acgtgcacgg acatttctct
    41101 tttcccaaag attggactgc ttcaaatatt tttcgctgcg tttcatcgct tctttgcgag
    41161 agacaccgta gtagcctgct tgattcacga cgatttgctg gaccgtttca aaagggttaa
    41221 aattgaactc ttgtggaacc aatccaatct gttgctttgc tcgaactaga tccttgtcca
    41281 gatcataacc gaagacattc acttttccag atgttttatt gactaaagaa gtaataatcc
    41341 cgatcgttgt agacttccca gctccatttg gccctaatag cgcatagaaa tctccttctt
    41401 tcaccgaaag atcgattccc cgcaatgctt cgaccccact tccatagatc tttttcaaat
    41461 tatttatttc aagcgcattt gccatttatt tcctatctcc ttatagacat gattcttaat
    41521 tgtatcatgg aatacagcag atgaaaaaac aacttggctt ccacacaaaa acacacctgc
    41581 taacaaagag gagatgtgtt ttctagttca tgatcatctt cgatagaata actatctaaa
    41641 caactctgga acttttaaaa tcataaaagt accgacagca actactacga tgacatatag
    41701 aagatcctta taactgaggt tcttgtcttt tttattcatg ttaagctttc ctccgtcctt
    41761 ttatcgaaag cttttttctt ttattttaac cgaaataaat aaaatggaaa tagttatttt
    41821 caatttctgt tttcgctgtt tgttttacgg agaactttgc catggaacgt gcgtctcgtc
    41881 gctgatgcta agttttttcg ataaaagctg ctagaaggaa gacagcgtgg tttattttgt
    41941 atcgtctttt gctaaatctt ccaagatcat ctggacagca gactcgttcc cattgaattc
    42001 tgccactttt ttggcatcaa tgtacttctt ttgataatcg tctttttgga cccacgcatc
    42061 atgcttcgtt tgataaagtt aatcaaacgg ataccactga cgctgttcat cgaacaacaa
    42121 actttctttt tgggcctgtt caactatgga tttaggataa ttcaaaactt tcagcaaggc
    42181 aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac tgattccttt
    42241 tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta aaatctctgt
    42301 caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa gccaccgaac
    42361 gactgaagcg gaagaagcaa tccgttcgat cgtatttgtt ccttttaaaa tctcatctac
    42421 gaagcagtag cagcgttctt tggaagccac ttggtccagc aaacgtttga tcgatttgat
    42481 ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca tggaagtcaa
    42541 cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg tctgtgctag
    42601 aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat tggaacccgt
    42661 gactaatgta ttcttctgcc agttgactgg attaactacc gcttctttta acaatggatg
    42721 atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag tgatcggcat
    42781 ataggtgcga taattcaaaa cggctgctgc aacttccaat tctcccagca attcccacag
    42841 cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat aggagataaa
    42901 tggcagcatg aacatgatgt tgacatattc aaaaatcaat tctgcttcac tcccgctctt
    42961 cattcgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt gctgtttcag
    43021 ctctttttga agtggtgtcg tcatttttgc taactgattg gcggctgaaa tcgttgaaac
    43081 caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta gataatagac
    43141 cgtattgaat actaagccac cgattgctag atagattcct agtacttgcc ctaaaagaag
    43201 caaacctaca cccaccagcg gaagcaagcc aagaaaaata aataaaccta cgcgtccaat
    43261 ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt tttttcctaa
    43321 acgagcgaat tgatattgga ttctttctct cagttgagga ttttcctgat aaaaagcaat
    43381 tagtttttct aactgataat cttctccaaa atcaaagcta cgtaaacgct gatacaacgc
    43441 ttctgaacct acactagaat aggtactgtt gatcccttca aatacttcga acatatccaa
    43501 gtcataccaa gtaagatcgt cgatttcact atcccatgaa cggaattttt tctccgtgag
    43561 ccacgcatcc ttcaaacttt cttctttatc aaatctcggt tgataaggaa tttttcccca
    43621 attactacga actgtttcct ttactttcaa acgtgaaaaa atgtctaaaa caactaataa
    43681 aataacaact gcaataataa ttaaaacgat aaattctctt tggcccatat ccctctccgt
    43741 tccttgattt gttagtttta gtatagcact gttgcgctat ttgaaaaggg ggtacaaaaa
    43801 taactgtctt cgttacctaa ttcgtatcaa tcacaccaaa ataaaaacac cttccaaaaa
    43861 agaagatgtc tttaaaacga gatacaattt tttgtcattc acttcaggta aattagagcc
    43921 aattaaaccc tccgcaactt gacggagatg gagaagatgc ccccgtctaa tctagcggtc
    43981 atctctcctc ccattccttc caccaatttt tttgacaggt aaagcccgat tccgctagat
    44041 tccgtatttg tgcggctggt attttcggta taaaaacgct ggaagacttt ttcaacagag
    44101 gtttgcgctc ctggtttgat aggattggcc actgttaatc gaatgaatga atcctctttt
    44161 gaaaatgtca cctttgcttg tccatctgca taacgcaata aattctgtat gatattttgc
    44221 agcactcgca gcaacatgag cggatctgct aatacagttg tatttatcga gtgttcagcg
    44281 aacgctacct cgatattttt ctcagaaaat tgttcataaa aagagaaaaa tacttcttcc
    44341 acctctttag aaaaagaaag cgtatggaga tccatttctt tttgcttcgt ctctattcga
    44401 gcaagatcat aaaactgatc cgtcatactg agtaatcttg caacagattg tttgatgatc
    44461 gttaaatact tctcccgttt ctccgtatcc tctacctgtt ccagtaattg tacataaccg
    44521 ttaatagcag tcaaaggagt tctgagatca tgagaaaggc cgacgatcga tagtctgatg
    44581 ttttcttcca tttcttgaaa tgcttgtcct ttttgttcat tttcatccac ccatgtattc
    44641 agtgcatcta ctaggtccat tagattcttt tctcgaaatt cgactgaaag acgaccacca
    44701 tattcttgtc gttcagaaag agtggcgatt ttcttagtta atctcttgat ttccctttgc
    44761 aggaagaaaa taaaacttcc cataaagata acaacgagaa gcaacagcca cttaatcaaa
    44821 tctaaacccc actccccaca ccgtatgaat atacacagca tgtgcctttg ctagtttccc
    44881 tcgaagattg gaaatatgca tattcactgt tttctcacta tcaaaatatg gttcttgcca
    44941 aactgtttca taaagatttg ctcgcgaaaa tacttttttc ggatgttcta aaaaaagctt
    45001 cagcagatcg aattctcttc ctgtcaaatg gacagccaca ccattcacat ttacatccct
    45061 cgactctttg tccagtaaga tttccttgta atagatgcgt tgcgacagat cttttacttg
    45121 gacagaagtc tgatgacgaa gttggatgcc gattcgtaac aacagttctt cgatatcaaa
    45181 tggtttcgtc acatagtcat ccgctcctgc tgccaacaag gaaactttat ctctttgatc
    45241 gctttttgct gaaagaacga tgatcggaac attcgatgtc tgtcggattt ctgctaacaa
    45301 tgcttcacct gaaagccctg gaagcatgag atctagaagg atcaaatcga caggatatgt
    45361 gcttaacaat aattttcctt ccgtacctga ataagcatca agaagtttgt atctctgttc
    45421 aagaacctcc tccagtaaag aatgaacagc ttcatcgtct tcgataatca aaatagtttc
    45481 cagcaggaac acctacttaa tgtcagattt ttggaaaata aacattccta gagctgatga
    45541 taccacgatt gttactagac tgaccagaat cattttcggt gcttgcgaat aagttgcttg
    45601 ttgcgcaaag tccgtataca tgagccacgt ttccgtaaac cactccgtta gaaattccaa
    45661 gcctttgatt tttgcgaaaa caccgggaac gaaggccgtt cccatgatat aaagaaaaga
    45721 aagaagcaaa ctgacactgg tacttctagt gatgaatgtc aaagccactg gaacagataa
    45781 tgtcgctaat atcagcagaa gcaatacagg aaatactttt agaacataat ttccatagtt
    45841 tttgattccc tcaacttcac caaatccgaa agcaagtgtg taagcaatca tggctagccc
    45901 cgtaatcaaa accacgccaa gtgttgtgat caaaagcgta acgagaagtt tcgacacata
    45961 ataagtcatt cgattccgtc ctaaagataa cgtattgcga atcgtaccgt tttgaaattc
    46021 ttccccaata taaaagcacg caaatgctgc gaaagccaga agaatccatg cagccatttt
    46081 ttccaccatt aaaatccctg ttgcaccgtt gatgactgta tccgttgctt ggtttaattc
    46141 attgccgtat tttgtatgca tataccattc gttccaagaa taaaaaagcg gaagtacgac
    46201 catcagtcct agtgagaccc acattttacg gctcttccat acttttattt tttcagcagt
    46261 taatagatta aacatgtgga ttgcctccag ttaattgtaa gaaataatct tccagttttt
    46321 gcccaactag gtggatacca tccacaggaa cttttgcctc taccaatgct tgattgatgg
    46381 ctgctacttg gtcaattcct tcaaaaagct ggataacacg gctagattgg acaaaataat
    46441 ccctataacc aagagagtcg agaaccgtta ctgcttgttc cgtcgccgaa gtgtcaattt
    46501 ttataaattg acgagattct tgtgccaatt cttctttcga taattctttg atcaagtgtc
    46561 cttcatgcaa aataccgtag tgagtggcga tctgtgacaa ttcatctagc aagtggctag
    46621 aaagcaagac ggtgatgccc cgttctttta ccaaacgttg gatgatttct cgcatttctg
    46681 ccatccctgc tggatccatc ccattcgtcg gttcatccaa aatcaaaaat tctggattcg
    46741 tgatcagtgc atttgcaata gctaaacgct ggcgcatacc taaagagaaa ttctttgctt
    46801 tctttttccc tgtattttcc aatcgcatca ttttcagcag gtcttcgatt tctctatccg
    46861 acacaccgcc gttcgctgct tggatacgta ggttatctct tgcagttaat tctggataaa
    46921 gagcaggggt ttcaatcgat tgacccatac gtcgtcttgc tgcctgcaaa tccgtcgttc
    46981 caaataattc gatcgaacct tcatcaatcg agatcaaacc cataatagtt ctcataaagg
    47041 tagacttacc cgctccattc tccccaatca aaccatagat cattccacgt ttgatttcga
    47101 tcgatacttt atctaatgct ttggcagcac cgtatttttt ggttatacca gttgctttca
    47161 agaccgtttc atttccaaaa tccatactta tccttctttc ttattgataa gtgaagtata
    47221 gttgacgggt ataaagaaag cagccagaaa tgtataaaga aagtgtaaat tttccaattg
    47281 aatttcaaga aaaatcagac tgtctcgcag acgtggttga gttgtctgcc aatcaaagag
    47341 cgcaccaaac agtgctaatc caagtggact ggccaccatg gtaatcgttg ttatcgttga
    47401 catcagacgt ccttgtatat gcgttggaac ttctcgctga agccagacca tataaaaagc
    47461 aaaagaaaac attaatcctt aaaaacggac ttatgaaaaa taatacatgc cgtttgggat
    47521 ttacaattag ctaccaaata agaggttatg acaaaagact tttcacaatc ccttcttccg
    47581 aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatattta tcaaacatga
    47641 gaagcaattt ttgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
    47701 agaagcggat aaataatatt gtttgatgat actttatctt tttcccgttt gtttatagat
    47761 cgaatgatat tatagaccaa gaatcctacc tattaacata gctaaaaaca caaaaaaggc
    47821 actttccaaa ttgtggaaag tgccagaaaa gttaggatta aggtaactgc gctcagcatt
    47881 gcttcgccca gtacgattct cgtaactcgt gaactcgaac gataatcgca attaacgttt
    47941 tgagaactgt gaagctttac gggctttctt aagacctggt taaacaaaac tctattttat
    48001 tgcattttac aggaatgtat ttcatgcgaa attaataagt ctttgtttat agagttttac
    48061 tagggtataa aaagttgtat gttatcatta cctcaaaaca ttacctcagc ttggcgagtt
    48121 ggttgattga agaataaatc atttatctag atatctcaat aaaaaatgat ttctaagaaa
    48181 cttgtatttt cgtgttatta tttttttgcc ctatcaaaat ttctaattct tctagcgtaa
    48241 tatgattatg acacataatc gattcaatta cttcaatacc tatttttttc gagaaagatc
    48301 ccttttgttc actcacaaaa atactttcag aagacatata taaggaacga agttttggaa
    48361 ttagtatatc gtacaaatag accaccataa aatcgtcctt gcaaccattc tcaaaaagaa
    48421 ttaaatttgc tgattgtgtt acatatatca ctgaattaca ttcatggtca attccaaaaa
    48481 caatgtctac ttcattttct tttctaattc gcgcagttac atttttcaaa ctttcaccat
    48541 tacttataaa tgctttagtt actgtaagct ctgatgtttg ttcagatatt ccttttataa
    48601 tattttgttc atcagccaca ttccccataa atccgccgat tccttttaag taataattct
    48661 cacctaagag tcgcttgcat tcactgaatt gataaaaaaa ccagttaaaa aaatcttctg
    48721 gaaaaataaa ttctgatcga actttattta tatttttagc tcctattaat tttataactc
    48781 gattaatgtt cacttgctgc gatgaacata taactgaata aattgagttg tttaattcaa
    48841 aaaaacaact ttaacatatg catcattaat tcgttatttt ttaggtagat attgatcatt
    48901 gttatctctc tttttttgct tcccatactc caacttagaa attgccgtta taaaatttat
    48961 tttaaaggtg actttttccc ctgaagtatc ggtagtagga atacttaata cctcagcttc
    49021 tgaggaggta tcccaattat agttatactt tgcatcatca ccataaagaa caattccatt
    49081 ttttttatct tgtgccgtta gctttactgg ttcgcttact tttcttgtta atatcttgga
    49141 ctttacatca tcgatattgc ttattttatc aagcttaaat aatttacaaa ttaatttcgt
    49201 actagttttt atatttgacg agagtgtaaa atattttgtg taaatagaaa aaaggaagtc
    49261 ccttctgtag aatagagtta ccacaacaca ttcacagaaa agaggacttc catatgaacg
    49321 attttactac agaaattcta aagactctag cgaacaaagg cgatttgaat gaattattcc
    49381 gtgtccattt ggaaaaagct gtcaatacgc ttctcaaaac ggagttaacg gctttcctcg
    49441 attacgaaaa gtacgatcgc attggtttta acacggggaa ttctcgtaac ggctcctatg
    49501 accgtacggt caagaccgag tacggggaac ttcatctcca gattccgcgc gaccgcaacg
    49561 g
//
LOCUS       DAGCYL010000004        42327 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.004, whole genome shotgun sequence.
ACCESSION   DAGCYL010000004 DAGCYL010000000
VERSION     DAGCYL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.004"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            100..1164
                     /locus_tag="MB216_000146"
     CDS             100..1164
                     /locus_tag="MB216_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBT4525011.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            1410..1835
                     /locus_tag="MB216_000147"
     CDS             1410..1835
                     /locus_tag="MB216_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBT4525012.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            1828..2277
                     /gene="nusB"
                     /locus_tag="MB216_000148"
     CDS             1828..2277
                     /gene="nusB"
                     /locus_tag="MB216_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBT4525013.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            2374..3228
                     /locus_tag="MB216_000149"
     CDS             2374..3228
                     /locus_tag="MB216_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBT4525014.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            3229..4566
                     /locus_tag="MB216_000150"
     CDS             3229..4566
                     /locus_tag="MB216_000150"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBT4525015.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            4571..4795
                     /locus_tag="MB216_000151"
     CDS             4571..4795
                     /locus_tag="MB216_000151"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBT4525016.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            4797..5687
                     /locus_tag="MB216_000152"
     CDS             4797..5687
                     /locus_tag="MB216_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBT4525017.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            5696..6514
                     /locus_tag="MB216_000153"
     CDS             5696..6514
                     /locus_tag="MB216_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBT4525018.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            6621..7070
                     /locus_tag="MB216_000154"
     CDS             6621..7070
                     /locus_tag="MB216_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBT4525019.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            7088..8770
                     /gene="recN"
                     /locus_tag="MB216_000155"
     CDS             7088..8770
                     /gene="recN"
                     /locus_tag="MB216_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBT4525020.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(8825..8941)
                     /locus_tag="MB216_000156"
     CDS             complement(8825..8941)
                     /locus_tag="MB216_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBT4525021.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(9014..9970)
                     /locus_tag="MB216_000157"
     CDS             complement(9014..9970)
                     /locus_tag="MB216_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBT4525022.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            10141..10509
                     /locus_tag="MB216_000158"
     CDS             10141..10509
                     /locus_tag="MB216_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBT4525023.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            10679..11110
                     /gene="mraZ"
                     /locus_tag="MB216_000159"
     CDS             10679..11110
                     /gene="mraZ"
                     /locus_tag="MB216_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBT4525024.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            11131..12090
                     /gene="rsmH"
                     /locus_tag="MB216_000160"
     CDS             11131..12090
                     /gene="rsmH"
                     /locus_tag="MB216_000160"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBT4525025.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            12095..12496
                     /gene="ftsL"
                     /locus_tag="MB216_000161"
     CDS             12095..12496
                     /gene="ftsL"
                     /locus_tag="MB216_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBT4525026.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            12497..14689
                     /locus_tag="MB216_000162"
     CDS             12497..14689
                     /locus_tag="MB216_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBT4525027.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            14715..15677
                     /locus_tag="MB216_000163"
     CDS             14715..15677
                     /locus_tag="MB216_000163"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBT4525028.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            15691..17052
                     /locus_tag="MB216_000164"
     CDS             15691..17052
                     /locus_tag="MB216_000164"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBT4525029.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            17078..18166
                     /gene="murG"
                     /locus_tag="MB216_000165"
     CDS             17078..18166
                     /gene="murG"
                     /locus_tag="MB216_000165"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBT4525030.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            18181..19401
                     /locus_tag="MB216_000166"
     CDS             18181..19401
                     /locus_tag="MB216_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBT4525031.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            19557..20882
                     /gene="ftsA"
                     /locus_tag="MB216_000167"
     CDS             19557..20882
                     /gene="ftsA"
                     /locus_tag="MB216_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBT4525032.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            20905..22146
                     /gene="ftsZ"
                     /locus_tag="MB216_000168"
     CDS             20905..22146
                     /gene="ftsZ"
                     /locus_tag="MB216_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBT4525033.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            22163..22840
                     /locus_tag="MB216_000169"
     CDS             22163..22840
                     /locus_tag="MB216_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBT4525034.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            22854..23453
                     /locus_tag="MB216_000170"
     CDS             22854..23453
                     /locus_tag="MB216_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBT4525035.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            23468..23755
                     /locus_tag="MB216_000171"
     CDS             23468..23755
                     /locus_tag="MB216_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBT4525036.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            23804..24586
                     /locus_tag="MB216_000172"
     CDS             23804..24586
                     /locus_tag="MB216_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBT4525037.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            24607..25314
                     /locus_tag="MB216_000173"
     CDS             24607..25314
                     /locus_tag="MB216_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBT4525038.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            25576..28374
                     /gene="ileS"
                     /locus_tag="MB216_000174"
     CDS             25576..28374
                     /gene="ileS"
                     /locus_tag="MB216_000174"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBT4525039.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(28423..29949)
                     /locus_tag="MB216_000175"
     CDS             complement(28423..29949)
                     /locus_tag="MB216_000175"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBT4525040.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(29964..30611)
                     /locus_tag="MB216_000176"
     CDS             complement(29964..30611)
                     /locus_tag="MB216_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBT4525041.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(30795..31124)
                     /locus_tag="MB216_000177"
     CDS             complement(30795..31124)
                     /locus_tag="MB216_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBT4525042.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            31301..32029
                     /gene="sfsA"
                     /locus_tag="MB216_000178"
     CDS             31301..32029
                     /gene="sfsA"
                     /locus_tag="MB216_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBT4525043.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            32045..33058
                     /locus_tag="MB216_000179"
     CDS             32045..33058
                     /locus_tag="MB216_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4525044.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            33058..34335
                     /locus_tag="MB216_000180"
     CDS             33058..34335
                     /locus_tag="MB216_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBT4525045.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            34398..37100
                     /locus_tag="MB216_000181"
     CDS             34398..37100
                     /locus_tag="MB216_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBT4525046.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            37252..37569
                     /locus_tag="MB216_000182"
     CDS             37252..37569
                     /locus_tag="MB216_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4525047.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            37599..37919
                     /locus_tag="MB216_000183"
     CDS             37599..37919
                     /locus_tag="MB216_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBT4525048.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            38027..39487
                     /locus_tag="MB216_000184"
     CDS             38027..39487
                     /locus_tag="MB216_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4525049.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            39555..39776
                     /locus_tag="MB216_000185"
     CDS             39555..39776
                     /locus_tag="MB216_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525050.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            39807..39989
                     /locus_tag="MB216_000186"
     CDS             39807..39989
                     /locus_tag="MB216_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBT4525051.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            39989..40402
                     /locus_tag="MB216_000187"
     CDS             39989..40402
                     /locus_tag="MB216_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBT4525052.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            40525..41706
                     /locus_tag="MB216_000188"
     CDS             40525..41706
                     /locus_tag="MB216_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4525053.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAELGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(41777..41941)
                     /locus_tag="MB216_000189"
     CDS             complement(41777..41941)
                     /locus_tag="MB216_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525054.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            42095..42168
                     /locus_tag="MB216_000190"
     tRNA            42095..42168
                     /locus_tag="MB216_000190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42129..42131,aa:Arg,seq:cct)
     gene            complement(42237..>42327)
                     /locus_tag="MB216_000191"
     CDS             complement(42237..>42327)
                     /locus_tag="MB216_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBT4525055.1"
                     /translation="TYLHIVRELEDQDKEKINAVFDSLYKNDN"
BASE COUNT        14150 a         7192 c         9101 g        11884 t
ORIGIN      
        1 ataaaaagaa gttttccgga aatttttatt taactgtcgt gtacgaagaa aagtttgcta
       61 taatggaaaa aagcgtgcaa agaaagaggg agtttcatta tgatgctacg tgtagaaaaa
      121 ttgcgcaaaa aaatgcaaga agaaaatttg gattcatttt tggttaccag tccgtacaat
      181 cttcgttact taacgaactt tacaggtacg acaggattag ctgttattac tttggaaaaa
      241 gcatttttta ttactgattt tcgttatacc gagcaggcgg cagcgcaggc tcaagggttt
      301 gaaattatta aaaatgtcgg acctattttt gatgaggtag caaacttagt acgcaaagaa
      361 gaactgcact cattagcttt tgaggaaaca acagtctcat ttttagaata ttcggtttta
      421 gaagaaatca tcgatgcgga attagtacct gtttcacaga tgatcgaaga actcagagaa
      481 gtgaaagatg aagaagaaat cgctattatc gaaaaagcat gtcatattgc ggatatggca
      541 tatgatcaca tcctgaaaat gatccaaccg ggcatgactg agattgaagt cgcaaatcaa
      601 ctagactttt atatgcgttc tcttggcgct tcaggtgttt cttttgatac gatcgttgct
      661 agcggattgc gttcggctat gccgcatggt gtagcaagca aaaaaatcat cgaacaaggt
      721 gacttgatca cattggactt tggttgctat tacgaagggt acgtttccga tatgacacgt
      781 acattcgcta ttggtgatcc tggggaaaaa ttaaaagaaa tttatcaaat agtcttagat
      841 gctcaattag cagttattga tgctgcaaaa ccaggaatga ctggtgtcca attagatgca
      901 gttgctagag attatatttc aaaacatggt tacggggaag catttggaca ttctacagga
      961 catgggatcg gtttagagat ccacgaagga ccaaacgttt cttctcgtgc agaaaagcaa
     1021 tttgtaccag gaaatgtgat cactgatgaa ccagggatct acttaccagg aatcggtgga
     1081 gtacgtattg aggatgactt gttgatcaca gaagatggaa atcgtgtgtt gactcgttca
     1141 ccaaaagaat tgatcatctt atagtttcat caatagaaga agtaagcgta aaaatcagtg
     1201 ttcacagact ttgttataga aaatcactcg atttaatgat acactataga agaactttta
     1261 agctgtaaga gataataaag ttgggtgttt tctccaattc atagcagttt gcagctgatc
     1321 ggcggcagtt ttcttaccac gtgatcacta tgccgcatca tagagaattc ggatgtatct
     1381 cgtgcttagg caaagggagg aataaataaa tggcagatga aaaaaatttg attttaaacg
     1441 caaaccaaga tttaggagag atcgtgatcg ctcctgaagt catcgaagta attatcggga
     1501 ttgcagcttc taaagtagaa ggcgtatacg gaatgcgcgg aacttttgcc agcaatgtca
     1561 ctgaattatt aggacgtgca gctcacggaa aaggtgttta cttagcaaac gaggaagatg
     1621 gcttgaaagt agatctttac tgttatctag aatatggtgt ttcagttcct aaagtagcaa
     1681 tggaaatgca ggaacgggtc agccagcaag tccttttcat gacggatatc gatttatctg
     1741 aagtcaatat ccatgtagta gctgttgttc ctgaaaaatt agaacaacca acaatcgaag
     1801 aactttttga agacgaagag gaagagaatg aataaagaat tatcaagaca cgagattcgg
     1861 gaaatggcat tgcaggcttt atttccatta gacttcaatg cagacctaac aaaagaagat
     1921 gcgattttca acgcaatcga attagaccac cgcgacatga tcaacgaaga tgaatcagag
     1981 tttgttcctg tttatcttga tacgctagtt ggcggtgtct gtgcaaaaaa agatgaattg
     2041 gataaagtga ttgaaaaaca tttaaaaaac aattggcaca tcaatcggat ctcaaaaatg
     2101 gatttggtga tcttgcgtat cgcgattttt gaaatgactt atgtgacaga tgtaccagcg
     2161 gctgttgctt taaatgaagc aatcgagttg gcaaaaacat ttagtgatga ccgttcacgt
     2221 aaattcgtca acggtgtttt atccaatgta ttgaaagaat tagaagctgg ggcgtaagtc
     2281 ctagcttttt tctatgaatc atcataaacc tgttataaat cgggaaaatt ctatggtaga
     2341 atgaaaacac aaaaaatatc tgggagtggt tagatggcag aattgattaa cggaaaagaa
     2401 ttagctgaaa aaatgcaggc agagattgct gaaaaaatcc aaaaattgaa agataatggt
     2461 atccatcccg gattagtcgt tttattggta ggggaaaatc cagccagcca aatctatgta
     2521 agaaataaag aacgtgtagc aaaagaaatc ggaatccatt cattggtaga gcgttatcct
     2581 gagacgattt ctgaagacga actacttgca gaaattgaaa aatacaatca agatcctagt
     2641 tttcatggta tattggtcca attaccattg ccaaagcata tagatgaaga aaagatcctg
     2701 ttagctatcc gccctgaaaa agacgtagac ggttttcatc cgatgaattt aggcagactc
     2761 cttgcgggta aaccggacaa gattccttgt actccatacg gcatcatgaa aatgtttgaa
     2821 gcttattcta tcgatccggc gggtaaacga gcagtagtta tcggacgaag caatatcgtc
     2881 ggtaaaccga tggctcagct actcttgatg gcagatgcaa ctgttactgt tgctcattct
     2941 aaaaccgaaa atctggctga actggcacaa gaagcagata ttttagtcgt agcaatagga
     3001 cgtggacatt ttgtcacaaa agaattcgta aaaccaggtg ctgtagtgat agatgtaggg
     3061 atgaatcgtg atgctaacgg taagctgatc ggtgatgtga aatttgatga agtggagcct
     3121 attgcaggct ggatcactcc tgtgccaaag ggtgtagggc caatgactat cacgatgctg
     3181 atgtatcaaa ccgtaaaatc tgccgaagct tttggagcag gtgaatagat ggcacaagaa
     3241 tatttaactg tcactacatt aacgaagtat ttgaaaagaa aatttgatgc cgatccttat
     3301 ttagaacgcg tatatttaac aggcgaaata tcgaatttcc gtttacgtgc caatgcccat
     3361 caatatttca gcttaaaaga tgaccatgca aaaatatctg ctattatgtt caaaggagca
     3421 ttccaaaaat tacgattcca gccaaaagaa ggaatgaaag tccttgtcgt tgggcgtatt
     3481 tcattatatg aagcgggagg ttcttatcaa atctatgtag agcacatgga gcctgatgga
     3541 ataggtgctt tatatcaaga attggaagaa cgaaaagaaa agctgatgaa ggaaggcctt
     3601 tttcaagggc ctaaaaaaat gctgccacgt ttcccaaagc gaatcgcggt ccttaccagt
     3661 ccaagcggag cggttatccg cgatatcatc acgacggtga aaagaagata tccaattgtc
     3721 caattggttt tgttcccaac agttgtacaa ggaaaccaag cagcggacaa tgttgtcagg
     3781 aatatccaga gagtagaaga gatgggcaat ttcgatacaa tgatcatcgg tcgtgggggt
     3841 ggttcgattg aggatttatg gccgtttaat gaagagcggg ttgctcgggc gatttatcaa
     3901 gctaatacgc cggtgatttc atctgtagga catgaaacag ataccacgat cgctgattta
     3961 gtagcagatg ttcgggctgc cactccaaca gcagctgcag aattagctgt tcctgttttg
     4021 tcagaagagt tgatgcgcat tcaagaacgg caagctagat tgcagcaggc gtttgtacgc
     4081 cagatccagc gcaaacaaga acggttcgag cgtgctcgac gttcctatgt atttcggcag
     4141 cctgaaagat tgtatgaagg tcagaccatc aaattagacc agttgaaaca gcgattatat
     4201 caagctacac agcaaattta tcatgcgaaa gaaaaacaaa cgatcgcatt aagccaccaa
     4261 ttggaacaag tggctccgct ttatcgagtt aaagcagctc gccaagaaac tgcttatcta
     4321 gaaaaacgct tagaagagaa aatccaacaa tatatgaaac aacagcagca gcgttttgct
     4381 caagctgttc aatctcttga tttgttgagt ccattgaaaa tcatgggaag aggctacagc
     4441 tatacgacgt taaatgatca agtagtaaaa agtgtcaccg agattcatca aggggatttg
     4501 ctgacgatcc attatcaaga tggaatcgtt aaaggacaag tacaagagat agacaaagga
     4561 gaataaaaat atggaaaatc caacttttga agagtcactg caagaattag aaaaaattgt
     4621 catgcagtta gaacaaggag acgttccatt agaatcagct ctagattcgt ttaaacgagg
     4681 aatggaatta agcaaacatt gccaagatac attagctaaa gcagaagaga ccttgacgaa
     4741 aatgatgaca gaatcaaatg aagaagttga ttttgacgga aacgaggaaa gctgagatga
     4801 gattaactga cttttcagct attcatctac ctgctgtaga aaaagaaatc ttgtcttttt
     4861 tagatgagca tacaacggat agaggactct atgatgcgat gagttattca gtgaaagcag
     4921 gcgggaaaag aatacgtccc ttattacttt taacggccgt tgcttccttt gatgagccta
     4981 ttgatgttcc cgtctatcaa gtagcagctg ctttagaaat ggtgcatacg tattccttga
     5041 tccacgatga tttgcctgcg atggacaatg atgatcttcg tagaggaaaa cctaccaatc
     5101 ataaagtatt cggcgaagca ttagctatat tggccggaga tggtttgctt acaggagcat
     5161 tccagctaat cagtatggca cacttaggta attctcctaa gcttctgctg cttcagcaat
     5221 tagctgtttg tgcgggatca caaggtatgg ttgctgggca agctgcagat atcgagggcg
     5281 aatcgaaaaa gctttcttta gaagaattag catttattca tgaaagaaag acagggcatt
     5341 tgattcgtta tgccttgctg gctggtggca tattagcgaa gcaacctgaa gaaatcttat
     5401 tgctacttca acgattagcc gaacatttag gtttagcttt tcagataaga gatgatctac
     5461 tcgatgtgat tggtacaacg aaaacattag ggaaaacagc tggtaaagat gaacgtatgg
     5521 aaaaaaatac ttacccgcgt ttattaggcc tagaaaagac acgtgaggcg ttggagattg
     5581 agctgttatc cgccaacaag atcatagaca aattagaaga ggacgttttt tcttttgatg
     5641 gcgggctgat gcgccagatg atcaagcaat ttgatgtgga gaaatgaggg aaagaatgga
     5701 aaaagaacgc gtagatgtat tagcggtcaa acaaggatta tttgaaacaa gagaacaagc
     5761 caaacgttca gtgatggctg gtttgattta caacgaaaaa aacgagcggt ttgataaacc
     5821 gggagaaaaa attccagtga gtagtgaatt aagagtcaaa ggtaaaaaac ttccttatgt
     5881 ttctcgaggg ggattaaaat tagaaaaagc actcaaagca tttgatctat ctgttgaagg
     5941 aaaaacattg ttagatattg gtgcatccac tggcggattt actgatgcag ctttgcaaaa
     6001 tggcgcaaaa atgagttatg ctttagacgt tggctacaat cagctagctt ggaaaataag
     6061 acaagatcct agagtcgtcg taatggaaag agtgaatttt cgttatgcaa aacccgaaga
     6121 ctttacctta ggaattccag aagttgctgt tatcgatgtt tcttttattt cactaaaact
     6181 gatgctgcct ccattacatg ctattttaaa agaagaggga gaagtgattg ctttaatcaa
     6241 accccaattc gaagcaggca gagaagctgt agggaaaaac ggaattgttc gtgaccctca
     6301 gacccatcaa aaagttttag aagacatttt gtcatttgcg gcacaaggtg gatatgatgt
     6361 gctggaatta agttattctc cgatcacggg gggagaagga aatatcgagt tcttggctca
     6421 cttgaggaaa gtacccgaaa gcggaacaat caattcagct attaatatgg cagaagtggt
     6481 ttcaaatgca cacgaacagt ttgaccataa ataaattgta actgtgaaaa tcaagtgaga
     6541 agtatgcgga ttcttcttta ttataccaag gaaataagct atcattgatt aagaagaata
     6601 tgctaaggag cgaagacgta atgagaaaaa aagatcggca tcgtttgatt actcgattgt
     6661 tgagtgagca agatatccga aagcaggaag aatttgttga aatcctgaaa agcaaaggaa
     6721 tatctgttac tcaagctacg atttccagag atataaagga actgaaactg atcaaggtac
     6781 cggcttcaga tggcggctac cgttatagca taccagctga aacaaacgaa gatgtaaata
     6841 ctaaattgga aaaactatta aaagacgctt tcgtggcagt tgaccaaatg gaaaaatttg
     6901 ttattttgaa aacattgccg ggaaatgctt cggcagcagc aaatttgatt gataaacgtt
     6961 acaagaaaga attattttcg attatcaatg atgacgataa tgttttgatg attacacgaa
     7021 ctgaagacca ggcagtttta ttgaagaaag acttcctgca ttacctttaa gttgaggagc
     7081 gaatgtgatg ctacaagaaa tcagtattac taactttgcg attattccgg aattgcggct
     7141 ttcttttcac gaaggaatga ccgcgctgac cggagagact ggtgcgggta aatccattat
     7201 tatcgatgct ttaggtctgt tagcaggagg aagaggttca agcgattata tacgtcaagg
     7261 agcagaaaaa tgtgtgcttg aagggttgtt tgaactacca aaacaagaag gattctctga
     7321 actgatggtt gaattaggga tcgaaacaga tgaggacaat ctgattgttc gacgggatat
     7381 gtccttaact ggaaaaaatg tttgtcgagt gaatggtcat attatcacat tagctaatct
     7441 gagaagaatc ggcagttatt tagtagatat ccaaggacaa aacgaacatc aggaactgct
     7501 ccagcctgaa tcacatcttg ctttattgga tcgttttgga gatgtagctt tccaacagaa
     7561 gaaaaagagt tatcagcaag aatatatctc ttatcgtgag ctggaaaaaa gggtacgaaa
     7621 agtgcagcaa aatgaaaaat cctatgttca gagaatagat atgctccatt tccagcaaga
     7681 agaaatagct gctgctgatt tgcaagtcgg agaagaagag aaattaaaag aagaaagaga
     7741 aaaacttagc aattatcaaa aaattgctga tggattagct gcaggttacg gagcactgac
     7801 gaataatgaa caaaacagtg tagatggtgt tggcttggcc gtttcagaac tgcaagggat
     7861 cgcccatcta gatgtggagt atgaagcaat ttatgaaaat atccaaagtg cttactattt
     7921 gctgcaagat gcaatcggcg atatgagtcg tcagattgat ttattggagt tagatgagag
     7981 tcgtttagaa gaagttacgc aacgtttaga attaatccga cagttgaaac gtaaatacgg
     8041 cgaatccatt gagtcaatat tggcttacta tgacgaaatc accgaagagc tagcttcttc
     8101 tgatttttca gaaggtcaat tggacaaaat gaaagaggaa ctagagcaaa aagagcttct
     8161 acttcagcaa caagccgcag atcttcatga agcgcgtaaa aaaatcgcaa aagaattgga
     8221 aaaatccatt ctgcatgaat tgaaaagtct gtatatggaa aacactgagt ttgaggtacg
     8281 tttcttaaaa gaagaaaata gacaattgaa tcatgatgga tttgaccaaa tcgaatttta
     8341 cattacgacc aatccaggag agccactgaa gcctttggtg aaagtcgctt ctggtggcga
     8401 actgtctcgg atgttgcttg ctttgaaaac tattttttct tctgagcaag gtgtgacaag
     8461 tattattttt gatgaagtgg atacaggtgt gagcggaaga gttgcacagg ccatcgcaga
     8521 taaaatttct caaatatcga aatactctca agttttgtgc atcactcatc tgcctcaagt
     8581 tgctgcagtc gccgactatc aatattatat tgtgaaagca gtgattgatg gacgtacgca
     8641 aacttctgtt tcagagttga aaacaaaaga aagagaagaa gaaattgcac gtatgctggc
     8701 tggaagtgag attacaaaac tcacactcga acatgcaaaa gaactattga aactcgcaaa
     8761 aaggacctga gccacctcgc agtggttcag gtccttttca gcagctgata gctatatttt
     8821 atttttacat gacactcatt acagcagtca atacaacaga tccaatcgtt aaaatcagca
     8881 ttgtccaaat gacaaatttt gttactttag caaaagtact tgtttgtttt ttttcacgca
     8941 ttgttttcaa cctcgtcatt aattagttga ttcaagtgta caatcttttt tccatttcca
     9001 caagagggct tttttataaa aacttccctt tttttaaata ataggtagtt acgatacaaa
     9061 taaataatgt taatagaact agccatataa aggcatcatc cctttcagca ataggtaact
     9121 taatgttcat tccatagata ctgccgacga ttgtcggtat agttaaaata atcgttaaag
     9181 aagttaaaat tttcataata ttattcagat tattcgatac gattgctgaa aaagtctgat
     9241 tcatttgttc tactagtttt aattgaatgc gagaagtagt cattgcctgc tctgtttcca
     9301 ccaatacatc acgtaaatga tttttcaaag catgattttc ttggaaaagt ttattttgcg
     9361 ctagttcttt caatgcatct aaattagcag aagtcgcttc tctgaaataa acgaggcttt
     9421 tttggatatc catgatttga taaagttgtt tgttttcagt ggatacttga agtttggctt
     9481 ccaaagtatc gcattgagat tgaagcgtag ctaaataact gttgtaagag agtgtgacct
     9541 gccaaatgaa ttgaaagaag atattgagat taggcgaaat atcacttttt ggaaaggtct
     9601 ggctaaagac tttctttaaa aacagtggtt catgattcgt aattgtgata agcttctttt
     9661 tgggcgtcaa aataatagag agcgggtatg tatccacttg aaaataaccg ctaggacttg
     9721 ttttagcaaa aggatattgt aacaataata acgctggctt tgtcaacttt gattgattga
     9781 attcttctgt acgggaattt tctgcatcgt ccaatatgga agtgatgtaa tctgacggaa
     9841 tttcaaattg atgtgtcagc tgatgaatct cttcttgatt aggacgttct acacaatacc
     9901 agatcgtttg gtcagtttcc tttccggtat tacgcagttt ctcattatct aatgtaaaat
     9961 atttgatcat gattttcctc ctgttgcctg tttcttttat tataacggat agggaattgg
    10021 aatacatgga atgtgtttgt aagatataga atataactgc ataaatttaa ataaaatgaa
    10081 acagttgtgt aaagtatata cagtatactt gaagcaacga taaaaaagga gtttacgttc
    10141 atgggcacat tcacaccact tatcttgttt tggtatattt acccggtaat cgttttgttt
    10201 ggttgccaat ttcttgtaaa attattttcg ttaaatcgtc gttttaaaat aaaagcccct
    10261 gatttggcga ttcctttttt gtggggcggt atgcatgcat tgtctagaaa tacagggaca
    10321 atttctatct taccgttttt ctttatttct gttttgctaa tgggaatttg tattgctgtt
    10381 ttccaagcat attattatga agaaattatt tatccgcgtt atttcaagat gacttggcgt
    10441 ttggtttttc tgttgacaat cgtcttgtac gctgtcttga tcattgtgaa catactttct
    10501 tacctatgaa aaaaatagaa aagctgtaaa aagtctttta aaaggctttt tgcagcttga
    10561 tttttaaaaa aatgtttatg aataaaaaaa gttgttcccg gatttcccag aaaaggtggt
    10621 agaaagtggg ggattgtggt agactaattt tagttagtgg aggaatgggg ggctttcaat
    10681 gttgatgggt gaattccaac ataatattga tgcaaaagga cgtttaatcg tgccctcaaa
    10741 acttcgtgaa gaacttggcg aaaagtttgt tctgacacgc ggattggatg gctgtctttt
    10801 cggctatccg atgagcgaat gggaaaatct ggaagctaaa ctgaacgaaa tgccgttggc
    10861 taaaaaagac gcacgtactt ttgtgcgatt cttctattcg gctgccactg aatgcgaact
    10921 tgataaacaa ggacgaatca atatacccag cacgttaaga aattatgcag ctttgacaaa
    10981 aggttgcgta gtcatcggtg tatctaaccg aattgaaatt tgggatgaga cacgatggca
    11041 agatttttct gcagcagcag aagagaactt tgacgaaatt gctgaaagta tgattgattt
    11101 cggactataa gaataggaag aggctgacta atgactgaaa catttcaaca ctatacagtc
    11161 atgttgaaag aaacggtaga tggattgaac atcaaaccgg atggcgttta tgtagactgt
    11221 acactcggcg gtgccggaca tagtgaatat ttattaagcc aactaagttc taaagggcat
    11281 ctgtatgcgt ttgaccaaga tcaaaaagca atcgatcacg ccaagctgcg tttagcttct
    11341 tatgtcgaac aaggtatggt cacatttatc aaagcgaact tcagagaatt agaagaacgc
    11401 ttgagtgagc atgtagataa ggtagacggc attttgtatg atctaggggt ttcctctccc
    11461 cagctagatg aagcagaacg tggatttagt tatcatcaag atgcaccgtt ggatatgcgc
    11521 atggatcagt cagctccttt atccgcttat cacgtaatta atgaatattc ctaccatgaa
    11581 ttagtaaaga ttttcttccg ctatggcgag gagaaatttt ccaaacaaat tgctcgtgaa
    11641 atcgaacgag tacgaaaaac aaaaccaatc caaacaactg gtgagcttgt cgaaatcatt
    11701 aaacaagtca tcccagctcc cgcaagaaga aaaggcgggc atcctgcaaa aagaattttt
    11761 caagctgtgc ggattgcagt aaatgacgaa ctcggtgccg aagaggcctc tttagaacag
    11821 gcaatccggt tattgaaagt caatggaaga atcagcgtaa tcacattcca ttcattggaa
    11881 gacagaattg tcaaaagtat gtttaaagaa tatagtacgg tacaagatct accaccggga
    11941 cttcctgtca tacctgacga attccaacca gaactgaaag tcattactcg taaaccaatc
    12001 cttccttcag aggaagagct aacagaaaac aatcgatcac gcagtgcaaa gttacgtatt
    12061 gcagaaaaaa taagaatgaa agaggagtag aatgatggca gaattaaaga aaatgcaaga
    12121 ttacgaatat gatttacctg aattggtgga tcagccggaa caacaaatgc ctgattcttc
    12181 ttcgaatcaa gaaatcattc ttccacaatc accggcaaaa cgattaaagc agatttctgc
    12241 tttggaaaaa attgctgtgg caagcgtggc gattgctatt gttgctttat gtgttttaac
    12301 agttatgctg cgcacaaata tcagcggagc tgaaaaagaa atcacgtcaa tacaaacaga
    12361 aatcaatgat aaaaagcaag aaaaaacgag tttagaacaa gaaaaaaacg aactttctcg
    12421 tactgaacgg attaaacaaa tcgcagaaga aaaaggattg tctattaatg acgacaattt
    12481 aaggaaagtg aagtaaatga gtcttaaaaa taaaattcgg cggtttatgg aaaagaaaaa
    12541 tctaaatccg atgaacaatc gcaaaaaagt aggtatcatt ttatttgcta cgagtattgg
    12601 attgttcttt ttatttgccg tccgtttctc gtatatcgtt attggaggtc atgttgctgg
    12661 tacctcatta gctgaaaaaa caaaacagtt gtaccaagga agtgaagtag taaaagcaaa
    12721 aagaggaacg atttatgatc gtaacggtgt ggcgctcgca gaagatgcct cctcttattc
    12781 aattaaagcg atactctcaa aaacatatac ctctggggat aaaaaactgt atgttgaaga
    12841 aaagaatttt gataaaatag cagaaatcct gcataagaat ctttctatag acaaaaaaga
    12901 tgcactgaat attttagagg acggcgcaaa aaaagagctg tatcaagttg aatttggtag
    12961 ctatgggaaa aatatctccc aagaaaccaa gcaaaacatc gaagcggaca tgaaaaaaga
    13021 aggtgtcgct ggtctttatt ttgtcgacca tcaggctcgg atgtatccaa atggcgtctt
    13081 ttcttctcat tttattggat acgcagttcc agacaaagat gagaatggct tagttggtaa
    13141 actaggtttg gagtctgctt acaatgatat cttgagcggt aaagatggta agattattta
    13201 tcaaaaagat aattttcaaa atcctctgcc aggtacagtg gcagaagaag aaaaagctgt
    13261 cgacggacaa gatatctaca cgactttaga cagcagacta caaagttatt tagaaacact
    13321 gatggatcaa gtaaatgaag aatatcagcc agaagaattg acagcagtcc tgatgaaagc
    13381 aaaaaccgga gaaatcttgg caatgggaca acgacctact ttcaatcctg aaacaatgga
    13441 aggattgact ggaaaagatg ctatctggcg taatttcttg gtacaagata gttatgaacc
    13501 aggatcaacc atgaaagttt tcactactgc agctgctatc gaagaaggag aattcaacga
    13561 gaatgaaacc ttccaatctg gaaaaatcca agtagaagat gctacgatca atgaccatga
    13621 ctttggtgaa aaaggtgtgc tgaccatgcg tcaagctctt tcatggtcaa gtaacgtcgg
    13681 gatggtcata ctggaacagc gattaggcgg acgatggtat aactatttac aaaaactggg
    13741 atttggacaa agtactcatt ctggattaga tgatgaagta aacggtgctt tacctacatt
    13801 aaatattgtc gaccgagcaa tgagtgctta tggacaagcg gtaggtgtga cgaatttcca
    13861 aatgatgaaa gctttcactt ctattgcgaa taacggtaca atgattcaac ctcgatatat
    13921 cagcaaagtg gtggatcccc aaactggaga agagcgaaca actcaaacag aagttctagg
    13981 gcaacctttt tcaaaagaaa caacagaaaa agtccgtgaa tatatgagag atgttgtcga
    14041 aagcgaaaac tacggtattg cttatggtgt atatagtgta cctggataca atgtatcggc
    14101 aaaaaccgga acagcccaaa tcgcttctga tacaggggga tatcaaacag gagatacagc
    14161 ctacctgtat tcgattgtgg agatggttcc ttctgaagat ccagactatg tcttgtatct
    14221 aacaatgaaa caccctaaga catatgatcg aatggcattg gctaaaatcg caaatccatt
    14281 gatgaaacgg gcaatggatt ttaaagaaac tgaagaagat tcagatacag aaacaaaaac
    14341 agaaaaagta tccgtagccg attatcgtaa tcttgaagcg gacgttgcag cagctgatgc
    14401 tcaaaaaagt ggactgcaac ctgtagtgat cggtaatggt aaaaaagtac aaaaacaatc
    14461 tactgccaac ggtgatcagt tgatctctgg agaaaaactg atcctatata ctggtggtga
    14521 taagctgatg cctgacgtaa caggatggtc aaaagcagat atcatgaagc tggggaaaat
    14581 cctcggtatt gaagttagtt ttgatggaga tggttattgt gtcaaacaag aacttgctcc
    14641 atatgaaaaa atcactgaag acaaacttag ttttacttta gaagaataag aagagaaata
    14701 ggagagaaaa gagcatggaa tggacacaag cattgattcc cattgtcagc agttgtgcaa
    14761 tgacaattgc cgcaatgccg ctatttattg gttattttca aatgaaaaag caaggtcagg
    14821 cgattcgtga ggagggacca aagtggcaca atagcaaagc cggaactcct acaatgggcg
    14881 gcttagtgtt tctgatcggc tctattttga caggaatttg ggttggagct tggcaaaaac
    14941 agctaactcc tactcttttc atcttgcttt ttgtattagc gttatacgga gtaatcggct
    15001 ttcttgatga ttttataaaa atattcaaga aaagaaacat gggactgaac tcgaagcaga
    15061 aattgattgg acagattcta gggggaatca tcttttatct tgtttaccgt tctgaaggtt
    15121 atccaggtgt attgaatttc ttcggactag atctaccttt aggaatcatt tatggtattt
    15181 tcgctctctt ctggcttgtt ggattttcaa atgcagtaaa tctgacagat ggcatcgatg
    15241 gattagttgc tggcttagga agtatctctt ttgcagctta cggtctaatt gcttggcatc
    15301 agcatcagta tgatgtatta atcatttgct taagtgtttt aggaggttta ctgggtttct
    15361 tcgtctacaa cagaaaacct gccaagattt ttatgggaga tgtcggttca ttagcgttgg
    15421 gcggactttt agcggcaatt tctataatgc taaaccaaga atggacattg cttttgattg
    15481 gtttgatcta tgttatggaa acagcaagtg tgatgcttca agttacttcc tttaagctta
    15541 caggaaaacg aatttttaaa atgtctccaa ttcatcatca ctttgaaatg tgcggatggt
    15601 cggagtggaa gattgataca gttttttggt tgatcagcat tgtgacttcg ttgatcacat
    15661 tatggatcgt ttggtaggag atgacgggaa atgaaaaaaa tatcaacata tgaaaataag
    15721 aaagtgcttg tcttaggact cgcaaaaagc ggagtaagtg cggccaaatt attacatgaa
    15781 ttgggcgcac ttgtcaccgt taatgatgga aaaccttttg acgaaaatcc ggaagcacaa
    15841 gagctgcttt ctttagggat aaaagtaata acaggtagtc atccaatcga attgttggat
    15901 gaagaatttt cattgatggt caaaaatcca gggattccct acagtcatcc tttagtagca
    15961 aaagcccaag aaatgggtat tccggtaatt acggaagttg agctagctta cgaagtagcg
    16021 gaatgcccaa ttattgggat tacaggaacg aacggaaaaa caacgacaac aacgatgaca
    16081 ggactactac tgaatgcagg agcagatcaa ggaattgcac gtttagcggg taatattggg
    16141 tatccagcaa gtggtgtagc gcaagaagca aaatcagaag ataaaattgt gatggaattg
    16201 tctagcttcc aactcatggg aatcacagat ttccgccctc atatagcggt gattaccaat
    16261 atctatgaag cacatattga ttatcatggt acaagaaaag aatatgtcaa agctaaatgg
    16321 aaccttcaaa aaaacatgac agaaaaagat tatctgatcc ttaactggaa tcagtcagaa
    16381 ctacaagaat tagcgcaaag aacaaaagca cgcgtactgc ctttctcgac aaaagaagtg
    16441 ttggaagacg gtgtgtatgc tgacgattac tcaatatact ataaaaaaga aaaaataatg
    16501 gaaatatctg aacttggcgt accaggtaag cataatgtag aaaatgcact agctgctatt
    16561 tctgtagcaa aattgtatgg tatatcaaat gaagctatac gagaaacgtt gcattttttc
    16621 catggcgtac cacatcgtac gcaatatgta ggagaaatcc aaggcagaaa attttataat
    16681 gattcaaaag caaccaatat tttagcgact aaaatggctc taagtggatt tgaaacgtct
    16741 aaggtcgtgt tattggctgg cggattagac agaggaaata catttgatga attaattcct
    16801 tcattaaaag gaatcaaagc aatggtcgta tttggccaaa caaaagaaaa gttgatggat
    16861 gctggaaaaa aagcaggcat cgagacaata gtaacagccg attctgtaga acaagcagtt
    16921 cctttagcat tagaaaacag tacagatggt gatgtagttt tattatcacc agcgaatgcc
    16981 agctgggatc aatacccaaa ttttgaaact agaggaaaca gattcatgga agctgtgaac
    17041 cgattaaaat agaaaaagta gaagcgagcg ataaaaaatg aaaattttgg ttacaggcgg
    17101 aggaacaggg ggacatattt atccagctct tgcctttgtc aattatgtaa agactcaaga
    17161 accgaatgct gaattcatgt atgtgggagc aaaacgaggt ctagaaaaca aaatcgtccc
    17221 tgatacagga atgcctttcc ataccttgga gattcaagga tttaaacgaa aaatttccat
    17281 gcataacatc aagacggtcc gattgtttct taagagtatc cgtcaagcaa aaaaaatcct
    17341 cagtgatttt caaccagata tcgttatcgg tactggagga tatgtttccg gagcggtagt
    17401 gtacgctgca gccaaaatgg gtattccaac agttatccat gaacaaaaca gtgtaccagg
    17461 tgttaccaat aaatttttaa gccgatatgt ggatcggatc gctctttcat ttaaagatgc
    17521 ggcttccttt tttcctgaaa acaaagcagt tcttgtaggt aatcctcgag cacaagaagt
    17581 agcagatact aaaaaatcag atattttgaa aacatttgat ttggatccag aaaagaaaac
    17641 agtcttgatt tttggcggta gtcaaggagc acttaaaatc aatcaagcag tatcagtttt
    17701 tttaagtgaa tatattcctt cttcttacca agttttgtat gcttcaggag atagatatta
    17761 tgaagagatc ctaaataaaa taaatcctga aatacaaaat attagtgtcc agccttatat
    17821 caaaaatatg gcagaggtca tggcttccag tgatctgctg gtggggcgtg ctggtgcaac
    17881 ttcgatcgca gaattcacag ctctggggtt accggcagtg ttggttccca gcccatatgt
    17941 tacgaatgat catcagacta aaaatgcaat gagtcttgtt cacgcaggag cagcaaaaat
    18001 gattgcagat aatgaactaa caggtgaatc actaagtcag acagtcaacg aaatcatggg
    18061 tgatgaagaa ttgcaaaagc aaatgtgccg agcttctaaa gaacaaggaa tccctgatgc
    18121 ttcaaaacgt ctttatgact tagtaaaaca aataatcaaa aaataaaggc ggtggtctta
    18181 gtgagtaaga aaaaagaacc aaaaaaattg tctcatccgt cttctgatga aaacaatttg
    18241 actccttggc aaaaggctaa ccgaaaatat ctggcagaac aagagatgaa aaaccaagag
    18301 ccaaaaaaag aagacataac attagataca cccctttcag attctgaaga agaaaaaacg
    18361 gttcaagagg aacagcaaga agaaagccag tccgagaaaa aaatcgaatt ttttgaagag
    18421 atcaagcaaa agaaaaacgg tggaccatat aacggttctt ttttgaaccg gctgcccaat
    18481 ttaaaaagtc aacggcacaa agtactattt cgtcggttag ctttgatcat cactgtattg
    18541 ggtataccgc tgattttttt gatctattat gtttctcctt atagcaaatt acaagcagtt
    18601 gcagtatcag gcaataagac agtcaacagc caagaaatca tctctgatac aaaattgtca
    18661 ttaggagaaa atgtttgggg acagtatttt catcgatcta cttatatcga tcgccttaaa
    18721 aaagcacagc caagaatcga aacagcaaat atacatttca aagggatgaa tgaattcgat
    18781 ttggatgtaa cagaatataa agagattgct ttgatcgcaa aaaacaatca atactaccca
    18841 gtgattgaaa atggaacagt attagatgaa aaagtagcaa atcctacaaa aaatctccct
    18901 atcttggaag agttcaaaga ctctgcaaaa atcaaagaat taacaaagca atacaatcag
    18961 ttatcatcag aattacaaaa agcaatttct gaaatcaagt acacgccaag agcaagcaat
    19021 aaaaatctga tccaattgaa tatgaatgac ggcaatcaag ttattgtaaa tatcaataat
    19081 cttgcgaacc aaatgaaata ttattcgcaa gtagccaaag atatggatga aaaaggtgtg
    19141 atcgacatgg aagttggtat cttctcgcat ccttacggta gcactcaaga aacggaagaa
    19201 tcgggccaat cggaagaaac aactgaaagt gaagaaaatg cggatcaaac aaacgaatca
    19261 agtgaagagg cagaaacgca aaaagcacaa acacctggat cgtctagcac aaacggatca
    19321 tctgattcaa atgattcaag tgaaacaagt caaatagaag ataatccaag tacaactccg
    19381 tcaagtttaa atgaaaattg ataaaacaac tttaaaaagt aagaatgtac tttctaaaaa
    19441 ccagtaattt atggtagaat tggaagcagt gaatatatct attaataagt atattttaaa
    19501 acggacgcta gttgttcgct tgtatatgga atagtaagta ggagggaatc ccattcatgg
    19561 caaaaacagg aatgtatgta ggccttgata ttggaacgac atctgttaaa gttgtcgtgg
    19621 ctgaatatat cgacagccaa atgaatatta ttggagtagg aaacgcaaaa tcagaaggga
    19681 ttaaccgagg cattatcgtt gatatcgata aaacggtcca agcgatacaa cgagcagtac
    19741 gacaagcaga agaaaaagca ggtatccaga tcaaaggcgt aagtgtcggt ttgccagcaa
    19801 acatgcttga agtagaaaat tgccagggaa tgattgcagt taacggagat tcaaaggaaa
    19861 tcaccgatga agatgtcaga aatgtggctt cagctgccct tgttcgttca atacctcctg
    19921 aacgtcaaat cgtgtcaata ttgccacaag attttactgt cgatgggttc gagggaatca
    19981 aagatcctcg tggaatgatc ggcgtaagat tagaaatgta cggtctgttg tttacaggac
    20041 ctaaaacgat catccataac atacgtaaat gcgtggaaaa tgccggatta atcgtcaacg
    20101 aaatggtgat tactccttta gcgctgactg aatcaattct ttctgacgga gaaaaagatt
    20161 ttggtacgat cgtcattgat atgggtggcg gccaaacaac aacggctgtt atgcatgata
    20221 agcaattgaa gtttaccaat cttgaccaag aaggtggcga atttgtcact aaagatatct
    20281 ctattgtatt aaatacgtca tttaacaatg cagaagcgtt gaaaataaac tacggggatg
    20341 catacccaga acggacatca ccagatgaag aatttccagt agatgtgatt ggtcagtcag
    20401 aaccagtgaa agtcgatgaa cgctacttgt cagaaattat ttcagctcga atggaacaga
    20461 ttttcaacaa agcaaaagat gcattggacc aaatcgaagc attagagttg ccaggtggcg
    20521 tcgtattgac aggcggagct gctagtcttc ctggtgttgt ggacttggca caagaaatct
    20581 tcggcgtaaa tgtaaaattg tatgttccaa atcacatggg gctgagaaac ccggtattta
    20641 caaatgtaat aagtatcgtg gattattcag caaacttgag tgaagtgtat caattggcaa
    20701 aaattgctgt tacgggtgaa acctcagcag ctcgtcaaat ggtagttgaa caagaaacaa
    20761 caaacacata tgagagctat gaagcacctg aagaaacaat ctacgatgag ccggaaccaa
    20821 aagagtccgg agaaaatgta aaaaataaaa tcaagggttt ctttacaaat atttttgatt
    20881 aattcatcct gggaggaata agatatggaa ttctcaattg ataataatat caatgacggc
    20941 gcagtaatca aagtcatcgg agtcggtggt ggcggtggaa atgctgtaaa ccgaatgatt
    21001 gaagaaaacg ttaaaggtgt tgaattcatt acagccaaca cagacgtgca agcattaaaa
    21061 aattcaaaag ctgaaacagt tatccaatta ggccccaaat atactcgtgg tttaggtgct
    21121 ggttctcagc cagaagttgg acaaaaagcg gcagaagaaa gtgaacaatc cttacgcgaa
    21181 gctttagacg gtgcagatat gattttcatc actgcaggaa tgggtggagg aaccggtaca
    21241 ggtgctgcac caatcgttgc aggtatcgcg aaagaattgg gcgcattgac tgttggtgtc
    21301 gtgacacgtc cattcacttt tgaaggaccg aaacgtggcc gttttgctgc agaaggtatc
    21361 gctcgtttga aagaaaatgt ggatacatta ttaattatct caaataaccg tttattagaa
    21421 gttgtagata aaaagacacc gatgcttgaa gctttccgtg aagcagataa tgttttacga
    21481 caaggtgtac aaggtatctc tgatttgatc acagcgccag gttacgttaa ccttgacttt
    21541 gctgacgtga aaacagtgat ggaaaaccaa ggtacagcat tgatggggat cggtgtggct
    21601 agtggagaag atcgcgtagt agaagcaacg aaaaaagcaa tctcctctcc attgcttgaa
    21661 acatctattg atggagctga acaagtactt cttaacatca caggcggttt ggatatgaca
    21721 ttgttcgaag cacaagatgc ttcagatatc gtagcaaatg cagcaactgg cgatgtaaat
    21781 atcattctag gtacatctat caatgaagaa atgggcgatg aaatccgtgt gacggtcatc
    21841 gcaacaggta tcgatgaatc aaaaaaggaa cgcaagtcta gccgaccagc tagacaagca
    21901 caaatgcaat cacctgctca aaagactgta ttagatatgg atcaagctaa accaacttct
    21961 tcagaagaag aaaacagttt tggcgattgg gatatcagac gtgaacaaaa tgttcgtcct
    22021 agagttgatg attccaattt tgacaatatc gaaaaaaaag aatttgatac attcaaccgc
    22081 gaagaaacaa aatctcatgg cgatgatgaa ttaaatacgc caccgttttt ccgtcgtaaa
    22141 aaataaagga gaggaagagc acatgattgc tgacaacttg cagaaaatca agcaagaaat
    22201 tcaggattcg tgtgctcttg ttcatcgtga tccgaaagaa gttacattag tagctgtaac
    22261 aaaatctgtt gaatccgctc aagctgaaga attggttaga gctggcgtta tgaattgtgc
    22321 agaaaaccga gtagataaat tattagaaaa gaaacaagat ttatttgatt ttcctgaaat
    22381 aaagtggcat ttaattggta atttacagcg cagaaaggta aaattgattg taaatgaaat
    22441 tgattacttt catgcgttgg acagtttaag attagcagaa gaaatccaga agcggttaga
    22501 gaaagaatta tcctgtttta ttgaagtaaa tgtttctggt gaggaatcaa aacatggaat
    22561 ttcaccagat gagcttttac cgtttgtgga atctttagct gcctgcacaa aaatcaaggt
    22621 tgttgggttg atgactatgg cgcctaaaga agcttctgat gagcaaatcc ataaagtgtt
    22681 tggtacgctg aaagaattgc gtgatcttgt tcaagcaaaa ggttatgccc atgcaccatg
    22741 tagtgaatta agtatgggga tgagtcaaga ctttcaaatt gcgattgaag aaggtgcaac
    22801 atttatccgt gtaggaacag cgctattcaa agacgaataa aggaggaaca aaaatgccgc
    22861 ttttaaacag aggagcaatt gctaattttt tcggattagc tgatgaggaa gagtacgaat
    22921 accaagatta tcctgaaaca acaaaaaagg ctgtacagcc aactaaaaaa aatacaacac
    22981 agaatcaatc aactcattct gtatctgctg cgaatactca tgaaaaacaa aaaacggcga
    23041 cttcacgtcc tgtaacgcat gaaagtattc agccggctga aaagaaaaaa gtaattgaac
    23101 tacatcaatc acctacaaaa actgtagcga aagaaagaac agcttcaaga acctctacac
    23161 aacctaaagc aaatcttgct ggaaaaatca ctgtaatgga accaagagct tattctgaag
    23221 ctatggcgat tgctaaacga atcattgctg gagaagcaac cttagtgaat tttcatttag
    23281 tagaagaaaa acaagcgaga agaatcgttg attttctaac aggaacagtt tatgcactag
    23341 atggagatat caaacgtgtt ggagatgaga tttttctttg tacaccgact gggatggaga
    23401 ttgacagttc aaccgctcaa tcttttgcag atgcgaattt gtttgatctg taagaggagg
    23461 aactacgatt tatactattg cactattact tcatttactt caacgggcag cctatttcta
    23521 tagcattatc ttgatggtat atgcattgct ctcttggttt ccaggcgggt atcaatcagg
    23581 gctaggacga ttattagcta agatctgcga accgtatatc agtttgttcg atcgtctgcc
    23641 tttgaggatt ggacccttgg atttcacaat tttaggggca atcctgatct tgaacttagc
    23701 cgtacaaggt ttagcacaga tcgttatatg gataactgtc catttagttt attgattgaa
    23761 gcaacaaaat ctttattctc agaatagata ggaggggaaa attttggatg caaatgtata
    23821 ccagcacttt cgggcagatg aacgtacatt tatcgattcg gtaggcgatt ggattgaaca
    23881 ggttcaagga caatatgcac cctatctgac cgaatttctt gacccaagac aatcctatat
    23941 tttagaaaca ctgattcgac aaagcagtga tttgcgtttc atgttttatg gcggatatga
    24001 acaggcagaa agaaaaagat gtttgatttt ccccgattat tacgaacctg tagaggaaga
    24061 ttttgaaaca gcattgtatg aaatccatta tcctagcaaa tttgccagcc taagtcatgg
    24121 gaaaatcctt ggaactttag tgggaacggg gatcaaacgc tcttactttg gcgatatcat
    24181 ctcggacggt gatcgctggc aagtatttat tgcaaatgag gtggatcatt ttgttgctgc
    24241 acaagtaaca aagattggaa aggttgctgt acgcttggaa ccaatcaaat acacagaaat
    24301 gattatgccg aaagatagct ggacacaaga aagaggaaca gttagttcca tgcgactaga
    24361 cagtgtgatc tctgagattt ataatatttc cagacagcgg tccaagcagc tgatcgaagc
    24421 tggtaaagta aaagtgaact ggacagaaaa tacaagacct gattttgtac ttgaactatt
    24481 agatattatt tcagtcagag gttatggcag agtccagatc caagaacttg aggggaagac
    24541 gaaaaaagaa aagtacaggg tactgttcgg tgtattacgt aaataaaata aaaaggtggt
    24601 ttactcatgg ctttaactcc attagatatc caaaataaga cattcccgac aaaaatgcgg
    24661 ggatataatc aagacgaagt agatgatttc ttagatttag ttgttcgtga ctatgaggaa
    24721 ttgactcaaa gaaatcgaga attagaaaaa gcagtcaaac attctgaaga aaaacttgag
    24781 tacttcaatg aattaaaaga tgctttgaac caatcaatca tcgtagctca agatacggct
    24841 gataaagtaa aaacaagtgc aagcaaggaa tccgaagtga tcgttacttc tgcacaaaac
    24901 aaagcagatg agttagttgc gaacgctgaa aaacgtgcac atcaattaac aactgatgca
    24961 gaagaaaaag caagaaaaat cttgacagat gcaacagaaa aagcaagaca gcttgcgaca
    25021 gaaactgaag atcttaagaa aaaaacacga gtattccatc aacgtatcag cttgatgctt
    25081 gagtctcaat tagaacaagt gaaaagtccg gaatgggatg aaattttaca accattctcc
    25141 agctacgtga ctgacagcca tgaagtgatc aaagaagttt tagcaaaaca gcttgacaat
    25201 gaaaatgata ctgatgtaaa ctcagacaca ggagtcatag aagcacctat gacggcattt
    25261 gatacacagg ctattgatat ctcgatcgtg cctggtgaaa atgaaaacga gtagaacaga
    25321 aaagcacttt gttattctta aagcgagccg atgagagtgg aagttcggta agacactgca
    25381 tggtgaaaga tcctctactt gagacctgct tttccgaaat tagagtaaga agcagcggct
    25441 gacctcgtta ttggtcacag agagtttgca ctagatagat gcaactaaat ttgggtggta
    25501 ccacggcatg ttcgtccctt gcggctgaac atgctttttt gtgtccggaa aacaaataaa
    25561 ggagtgtatg gttgaatgaa aatgaaagaa accttgcaat taggaaaaac aaaattccct
    25621 atgcgtggta atttgccaaa ccgagaaggc gagtggcaaa aagaatggga agaaaatcga
    25681 atctaccaaa aaagacaaga gttgaacgaa ggaaaaccta gctttgttct tcatgatgga
    25741 cctccatatg ccaatggtaa tatccattta ggacattcat tgaataaaat cagtaaagac
    25801 attatcattc gttcaaaatc aatgtctggc tatcgtgcac catatgttcc aggatgggat
    25861 acacatggat tgccgattga acaagtattg gctaataaag gcgtgaaacg taaagaaatg
    25921 tccatggcag aatacttgga aaaatgtcgt gaatatgcac tttcccaagt agataagcaa
    25981 cgagcagatt tcaaacgcct aggagttgca ggagattggg aacatcctta tgttacatta
    26041 gatccttctt atgaagctgc acaagtacgc gtatttggta aaatggctga aaaaagctat
    26101 atttacaaag gattgaaacc aatttactgg tctccgtcaa gtgagtcttc tttagcggaa
    26161 gctgaaattg aatataaaga tgtaaaatct ccatctattt ttgtagcttt caatgtcaaa
    26221 gacggcaaag atattcttga tgaagataca gcttttgtca tttggacgac aacaccatgg
    26281 acattgccag caaaccaagg tattgctgta aatccaaaat ttacgtatgt attggtggaa
    26341 gccgatggcc gtaagtttgt cgtagcgaaa gatttgctag aaacagtcca atcagaaatc
    26401 ggttgggaaa atgtaacagt tctaaaagaa tttgctggac aagaaatgga atatatgact
    26461 gcaagtcatc cattctatga acgtgaatca cttgtgattt taggcgatca tgttacttta
    26521 gacgctggta ctggattagt ccatactgct ccaggacatg gggaagatga ctatatcgct
    26581 ggtagcaaat ataaactatc tgtagtgtca cctgtagact caaaaggatt gtttacagaa
    26641 gaagcacctg gatttgaagg tatcttttat gacaaagcaa atcctatgat cactgatcta
    26701 ttaaaagaaa aaggagcttt gctgaagcta gatttcttta ctcatagtta tcctcacgat
    26761 tggcggacga aaaagcctgt gatctatcgg gcaactccac aatggtttgc atctatcgat
    26821 aaattccgtc agaatatttt ggatgaagta gaaaaagtag agtgggttat cccatggggt
    26881 aaaacacgtt tgtataatat gatccgtgac cgcggcgatt gggtaatttc ccgtcagcgt
    26941 gcatggggag taccacttcc gattttctat gctgaaaatg gagaagcaat catcacacca
    27001 gaaacaatcg aacatgtggc aaaattattt gctgaacatg gatcaattat ctggtttgaa
    27061 aaagaagcga aagaacttct tcctgaagga tttacccacc caggttctcc taatggcgaa
    27121 tttacaaaag aaaaagatat catggatgtc tggtttgatt ctggttcttc tcatgaagct
    27181 gttttacgtc agcgaccaga attatcattc ccagcagata tgtatttaga aggatcagac
    27241 caatatcgtg gctggttcaa ctcaagtatc acgactagtg tggctatcaa tggtgtagca
    27301 ccatacaaat cagttatttc tcaaggattt gctttagatg gtgaaggccg taagatgagt
    27361 aaatctttag gaaacatcat tgcaccagaa aaagtgatca aacaatttgg tgcagatatc
    27421 cttcgcttat gggtatctag tgtagattat gaagcagatg ttcgtgtttc tatggatatc
    27481 ttaagtcaag tggcagaagt atatcgtaaa atcagaaata cgatgcgttt cttattagcg
    27541 aatacagaag atttcgatcc taaaaaagat actgtttcgt acaatgattt acgttctgta
    27601 gacaaatata tgaccgttcg attgaaccaa gtaatcaaag aaatccgaga agaagggtac
    27661 gacaaatata acttcatgca tatttaccgt acagttatga acttcttgac ggttgatcta
    27721 tcgtcattct acctggattt tgctaaagat gtagtttata ttgaagcaga agatgattat
    27781 caacgtcgtt gcatgcaaac tgttttctat caaacagctg tagctttgac aaaattattg
    27841 actcctatca ttccgcatac agcagaagaa atctggtcat tcctgaaaga agaagaggag
    27901 tacgttcagt taagcgagtt tcctggttat gaagagtttg caaaccaaga agaattgatg
    27961 gatacctgga ctgctttcat ggacttccgt gataatgtct taaaagcatt ggaagaagca
    28021 agaaacagca aattgattgg taaatctttg gaagcaaaac taactgttta tccaaaacaa
    28081 caagttcgtg aaatgttaaa agctttggat gcagatattg cacaattatt gatcgtttca
    28141 ccagactact ttgaagttat ggcagcagat gaacaagtgc cagataacgc tatggtattt
    28201 gatgatgttg ctattacagt ggaaaaagca gacggggaaa cttgcgatcg ctgtcgccaa
    28261 gttcgtaaag atgtaggagt agatgaaaaa cttcctcatc tatgcggtcg ttgtgcaaaa
    28321 atcgttgaag acttctatcc tgaagctgta gctgaaggat tcgaagaaaa gtaaaaggga
    28381 aaagggggtg tgacatgttc tttgtcacac ccccttttgt tatcaatcgt ttaattttcc
    28441 acgttcacga taccagttat ctggttgcca gatccattcg aatccgtctt tttcaagtag
    28501 ttcgaaagct gctttaggtc ccattgatcc agctttgtag ttgggaaaat ccggttgtgt
    28561 ttggtcccaa gcttttcgaa tgacatccac gatatgccaa gattgagcga cttcttccca
    28621 atgagagaag ttcgttccat caccattcaa cgcatctaat agtaattttt cgtaagcttc
    28681 tgggctattt tcaaccattt ctgcactatt tctaaattcc aatttgattg gttccgtttc
    28741 aaatccttgc cctacttctt tcccattcaa ggataaagaa aatccttcag ttggctgaat
    28801 atagatcgtt agaacattag gtggaagtgt cgtatcgtca catggttcat cgacagatgt
    28861 tttgaataca ttaacaggaa cttgtttgaa tacgatgttg atccgagtcc ctttttcagt
    28921 caaacgtttg cctgtacgta catagaaagg aacacctgac caacggaagt tatcgatagt
    28981 gaatttgcct gcgacgaatg tttcagtctc tgattgagca tccactttgt cttcttcgcg
    29041 atagcccttg aattgtacac catccaattc accttgagca tattgcccgc gaacgaagtt
    29101 ttctaacact tcttcttcgt tataaatgcg gatagctttc aatgctttga tcttttctgt
    29161 acggatttct ttttcggaaa atgctactgg cggttccatt gcaagtaatg ctacaacctg
    29221 taaaatatga ttttgcacca tgtctttaag tgcgccacta tgatcgtagt agcccccgcg
    29281 atcttctaca ccgaggtttt cgccaaaagt aatttggata ttgtcgatat agcgattgtt
    29341 ccattgagcc tcaaaaatat tattggcaaa acggatagct gagatgtttt ggatcatttc
    29401 ttttcctaaa taatgatcaa tcctgaaaat atcttgttct gggaatactt ctctgatttg
    29461 ctggttcaat tcaaaagcag actggtaatc agaaccgaac ggtttttcta tgatcaaacg
    29521 attgaagcca ttttttgtca taatattttg tgatttcaaa tgagaaacga tcgttccaaa
    29581 aaattgaggt gccatagcca aataatagac acggttaccg tttaattgat agtgtccatc
    29641 taatcggtca gacaattctt ttaatgtctg ataatgcgtt gaatcattta catcatggga
    29701 ttgatagtaa aaatggcttg caaaggtagt tgcttcttca tctgaaggat tcaatgcacg
    29761 gattgtttct ttgacaactt cacggtagtg ctcatcgctc cagggacggc gagcggttcc
    29821 gattacagca aaatgctcgc ctaaatttcc ttttctgtaa aggcggaaca aagacgggta
    29881 caatttacgc tgggctaggt cacctgtacc gccgaatatc gtaaacaata catttttttc
    29941 attagacatg tggatacact cctttatatt ttatcgacaa aaatcgtgct ggccgcctta
    30001 aagctgacgg ggattttctt tctatcattt tggatagtga tcggtccttc ataagctgcg
    30061 atgtcaacga tctcatattc ttcgttgata ttcaggtcaa tagtgactaa gtaatccaac
    30121 aattcttttt catcaagcac tcgtgcgata cgcacttttg taccgacagg ataggactcc
    30181 aatgtttgtc ggcgttcttc atggacaact tcctcttttt taggaatcga tcctccatga
    30241 ggacaatgtt caggatgatt caaatagttt tctaaatgat ctgcaagatg ttccgaagtc
    30301 acatgttcca atacttcagc atcatcatgg acttcattcc agctatagtc taaatgttca
    30361 actaaaaaaa cttcccataa gcgatgtttg cgaatcagac tgctagcttt ttgcagtccg
    30421 cttgcagtca attgtacacc ttgataaggg gaatgttcta caagctcttc tttcacaagt
    30481 ttagaaatca tttcactgac agatgcagct gagacatcta gactagatac gatttgttta
    30541 ttgttgactt tcgttgtatc tccacctagt tctaaaatga ttttcaaata atcttctcga
    30601 tttggtgtca tattcgatca tcctttcatg gtaaaaaacc atcattagta agtgtaacaa
    30661 aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa ggatttttag acgtttttac
    30721 aacaaaaaac gaaagatgat agagaagact cattctttcg tttataaagg atatatatat
    30781 acattgggag ggatctattt ttcagataac aacttatatg aagtgtctgc agattcgtca
    30841 taaagagttt gttcgggata agaaaagata agataaaaac catactgatc acgtacgact
    30901 aaacgatatt catctatttc taccacagat gtgccaagag actgaccgtt gatcaaaaac
    30961 gtgtaatcag atgtaatctg taattgttgt ttttgctggc tatcactaaa gaaccatgta
    31021 ccttgatact ttgagagtcc ttctgacttt tccgcttgtt ttttcttttt caagtaaata
    31081 aaacttgaga gaacggtaat gagtgaacca agaaaataga tcattttttt tatcaccaaa
    31141 tcactccttt atgagaaaat aatcgcatag acatattctt tcttctttat ttcattgtac
    31201 cgaataaaag ctaaaatttc tctaagactt tcgactggtt tttttactgt ttttctacaa
    31261 attatgatag gatacatagt aggaagaaag gtgacataat ttgaagtatc agcaagtgga
    31321 aatagctttt tttattgacc gcccgaatcg gtttatagca cattgtatga aaaaagacgg
    31381 agaaataatt attgcgcatg tcaaaaatac aggtagagga aaagaggtcc tgataccagg
    31441 cgctgaagtg gctattgtat ttgctcctgg acctaaacgg aaaaccgcct atgatctgat
    31501 tgctgtaaaa aagaagggtg actggtttaa tatcgatagt caattgccta atcggctggc
    31561 aattgatggt attttagacg gcacgatcct tttaccagga ttaactggtc catttacggt
    31621 atacaagcgt gaagtaactt atcttcactc caaatttgat atttatttag agacggtggt
    31681 cggacaaaaa gcatttgttg aagtcaaagg tatgacactt gaaaataaag cgattggtgc
    31741 ttttcctgat gcgcccacac tcagaggatt gaaacatata ggagaattaa cgtatgccgc
    31801 acaagatggt tatgccgctt atgtcttgtt tattgctcag ttcgaacatc ttcatctggc
    31861 aacgatccat gaagaaatgc aaccagcact agctgatatg gttcgacatg cgcagcaaag
    31921 cggagttcaa atcctcgcct ataattgtca agtatcggca gatgaggtaa ctgtcaagca
    31981 ggccattcca tttgatgtag atatcccttt tgaagatcct atacaataga aagaaggtaa
    32041 aataatgatc aacttaggta ttatcggaac aaattggatt actcatcaat ttgtccaagc
    32101 agctttaagc acaaaaaaat atgaattgac agcggtatat tctagacatt tggaaaaagc
    32161 acaggaattt ggttctcatt atgaagggga tatcgaattc gcaacagatt tgaagacttt
    32221 tttcaatatt actcatatgg acaccattta tattgcttct ccgaatagtc ttcactttag
    32281 tcaagctaag caagcaattt tggcaggaaa aaatgtgata gttgaaaagc cagctttttc
    32341 gacgccaaaa gaaatggatg aaatcatcca tttggcaaat gaacacaatg tgtatttttt
    32401 tgaagcagca agaaatatcc atgaatcagg ttttaaaaag attgccgatt acttaccatt
    32461 aaaagacgag ataatcggtg caaatttcag ctatatgaaa tattcttcgc gctatgacca
    32521 agtattagat ggagaagaac cgaatatctt ctcgactcat ttctcaggtg gtgcattgat
    32581 ggatttaggt gtttaccttg tatatgcagc tgttgcttgg tttggaatgc caaaagaagt
    32641 ccattatttc ccaagaaaga tttctactgg tgtagatggt cttggatggg ggatcttacg
    32701 ttatgagaat tttgatgtat cgatccagcc aggtaaaatc ggtgattctt atttaccgtc
    32761 agagatttat tttgactcag gaacgctagt gatgaatgga gtcaatgcaa ttgaacaagc
    32821 acaatttcat gatcgcgcgc ataaagaaat cattgatttg gcggtaacag ctgcagaaaa
    32881 tccaatggca gatgaagcag aggattttgc caatgtcatg gctcatccga aagatccagc
    32941 ttggggattg ttgtatgaag agtggatcga gctagctaga aatgtgaacc aagtgattta
    33001 tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat aatgaggaag atttttaatg
    33061 aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa aatatgagga taaagggttt
    33121 atctcacctt ctcttattca acgagcagtt tttcagccac taagtaataa acaaagtata
    33181 gtagcggttt ctcctacagg ttcaggtaaa acactggctt atctttggcc tctactacta
    33241 aacgtggaac caggtgaagc aagtgcgttg gtcatttttg cttcttctca agaactagct
    33301 attcaagtag cagatgttgc acgtgaatgg ggaaaagaca aagagttgaa agtccaatct
    33361 ttggtgggcg gagccaacgt caaacgtcag attgagggtt tgaaggaaaa accagaaatc
    33421 ttgattggta caccaggaag aatattggaa ttgatgaaag caaaaaaaat aaaagcacat
    33481 caagtgaaaa caatggtatt tgatgaagca gaccagctat ttgacgctgg gaacagccaa
    33541 atcattgatc aaattcttca tcaagctcca acagagtatc agctagcttt tttttcagcg
    33601 acagcagatc gttcccttga aagcattgaa aaaatcactg ggaaaacgtt tgagacaatt
    33661 gacgtgacag cagaggacga ttctcgaaaa ggactgagac attacttttt acgtgtaccg
    33721 atacgaaaga aagatgacta cttgagacgt ctcacgcata tagaagattt tcatggtctg
    33781 attttcttta atcaactgac tgaattaggc gtaatggaag aaaaattgct gtatagaggt
    33841 gtaccggtag gaagtctggc ctctgatcag aacaagctac tccgaaaagc agcgctagaa
    33901 caattcaaaa agaaaatggt cagagcactt ttgaccacag atgtggcggc tagaggttta
    33961 gatattacag gtcttcctta tgtagtgaat gcagaagttc cgttaagtga agaagcttat
    34021 ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag gaatagtaat tacactcgta
    34081 caagataata atctaagaga tttaaaacgg cttttacgtc catcaaatat aacacttgaa
    34141 gagatttttt tatacggagg taagctgcaa actgagcagc caaccaaaga agatgcagct
    34201 cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa aggagcagaa cccagaatat
    34261 aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa aaaaagagag aaataaagga
    34321 gcaaggcgca aataagcgcc tttttatgct atgattatga aaacgggaac aaaattggtt
    34381 gccagaggag gggttatgtg aaagaacgtc aagaagagat cttgcagcta ttgacagaaa
    34441 accaacaagg attgacagca agtgatgtag ctgagcgctt aactatcgat cgaagcaatg
    34501 caagccgata tttaagtgaa ctatacaagg cccatcatat tgtaaaaacc gctggccgac
    34561 cagtcgttta ctctttgcca acggaaaagt caaaatctga tgaagtgcat gtcgactctt
    34621 ctacgcaagt gacttttgaa acactcgttg gagaaaatga ttctttaaaa gtcagcatac
    34681 agcaggcaaa agctgcgatt ctttatccac cgcgggggtt gcatacgatt atttttggag
    34741 aaactggtac agggaagtcg atgtttgctg aatgtatgta tcattttgca atcgattcag
    34801 agatgctttc tgcagatgcg ccatttgttt ctttcaattg tgcggattat gctcaaaatc
    34861 cgcaactgct ctttggacac atctttggga tcaaaaaagg ggcttacaca ggagcagctc
    34921 aagatagtcc cggtctaata gccaaagcag acggcggtat attgttttta gatgagatcc
    34981 accgtttacc gccggaaggg caggaaatgc tgtttacttt cattgataaa ggtatttacc
    35041 gtccgcttgg agaaagtgca caagtacatg aagcatctgt tcagatcatt ggggcgacga
    35101 ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg gattcctatg gcaatcacct
    35161 tgccgaatct tgcttcacgt tcactggatg agcgttatga aatcatctct ctttttatca
    35221 aacaagaagc gaatcgtttg aatcaacgta tcgatatcga aaaagaagcg attcttgcct
    35281 tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa acgagacttg aaactagtct
    35341 gtgcaaaatc ttttttacat taccgtactc atcatgagaa aaagctaatc attcgaaaag
    35401 aagaactttc ccttcaagta caaaaaggat tactgaaaat caaggaaata ccggaacgtc
    35461 tcgaccgatt catggacagc aaaagtcagt atttgacatt tgaaccagga tttgctgatg
    35521 ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta taacgatatt gaggaaaaag
    35581 tcacttctct ttccgaaacc ggtgtggaga acattgatct agagaccctc atttcaaaag
    35641 atgtggatgc ttatttccag acatatgtca aagaattgac taaaagttcg attcccaaag
    35701 atcttttgcc agatgaaatc tggcggttaa ccaatcaatt gtatgatgtg gctgaaaaac
    35761 ggttagaccg tatttataat gaaaaagctc gttttgcttt tgctttgcat ctgcaaagta
    35821 cattggatcg tgtgagagaa ggtcatatga tcgtccaccc agatttgaat aacgtacgga
    35881 aaaattttaa gagggaattc caggtagcaa tcgatctttc aacgatcatt gaagaagaag
    35941 aacaagttga gattcctttt gatgaaatcg gctttattag tatgtttctt tctatcaatc
    36001 taggagaaag tgagcctgtt aaagaaaaca aagtagatgt tctcatattg atgcacggca
    36061 gacagactgc ttcaagcatg ttggagatgg cacaagaact attaggaaca aaagtaggga
    36121 aagcttttaa tatgccactg gttatggaag ttcaaacaat gtatgaagaa gtgttacaat
    36181 atgtcaaaaa ctatcaagag catttgacga atggcctact cttgttaaca gatatgggat
    36241 cattaaacac cttcagtgat ttgatttatg aagagactgg cattcggacg aaagctatca
    36301 cgatgacaag taccatgatt gtgatagaag cgatacggat ggcaagtgtt ggaagaagct
    36361 tagaagatat ttatcaaaat atccagcttt cctttgaatc gatcgtacgt gatcagttca
    36421 aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt aatcgtgaca tgctttactg
    36481 gtgaaggagt agctgccaaa ttatatcagc gtattatgcc agtgattaat caaaataagg
    36541 tagaactgat ccagatgcag tttttagaac gtgaatcatt caaaaaacac atcgatgcct
    36601 tattggaaga atatgagatc aaagcaattg cgggaacagt tgatgttgat tatcaaaata
    36661 tcccattttt ttcagcctat gacgtttttg atgatgaaaa actaaatatt ttaaagcgta
    36721 tagctgatga tgagatcccg acagctaaaa ttgtcgattc acttgctgga tcattaagcg
    36781 atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa gacagttcag cagatccaga
    36841 ccgatttgtc gattatcgtg gaacctggag tagatactgg aatcgttatc catttagcct
    36901 tcatgttaga cggattgatc aaacaagaaa gccatcggca atttgagaaa ctgtctattt
    36961 tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc aaacttgatg gcactagaac
    37021 gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt tcttacacaa atgtttcttg
    37081 aaaataaaat aaaaaaatga acacactaat catactgttt acacactaaa catctgtgtg
    37141 cgcactataa aaaagagcta aaaagcatga aaacgctatt tcagctcttt tttttattgg
    37201 catgcttctt gcgttatata aaagtgtaaa aaaataaaag gagtgttaaa aatggcaaaa
    37261 aaaacaatca tgttagtttg ttcagcaggg atgagtacga gtttattggt cacaaaaatg
    37321 caaaaagcag cagaagaaaa agggatggaa gccgatattt ttgcggtctc tgcctcagat
    37381 gcagataata atttagaatc taagaatgtc gatgtattat tattaggtcc tcaagtacgt
    37441 ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag gtattccatt agatgtcatc
    37501 aatatgcaag actatggcat gatgaacgga gaaaaagttc ttgcacaagc agaaaatcta
    37561 atgaaataaa tcaagtatgc agttgggggt ataacaacgt ggaagatcaa caaaatctag
    37621 aagccatcat ggggttaatc atgtttggcg gtaatgcaaa gagcgatgca atggaagcga
    37681 ttgcggctgc taaaatcggt gattttgagt tagcggatag gaaaatcgct gatgcagaag
    37741 aatcacttat acaagcgcat catgcacaaa cagacatgtt gacacaagaa gcacaaggaa
    37801 atcatatgca agtgacatta ttgactgttc acagtcaaga tcacttaatg acatctattg
    37861 cgtttactga tttagcaaaa gaaatcatcg aactttatcg tcgcgtatac agcgaataaa
    37921 tacaacttct tttttgaacg gaagaagaaa aatttttgaa cggatttttt attttgttat
    37981 aaggagaact ctctttatat aaaaaactta taggaggaag taaaaaatga acggactatc
    38041 aagttggatg gagaaataca tcctgccggt tgccgcaaaa atcggagcgc aaaaacattt
    38101 agttgctttg cgtgatgctt ttatcggcac actgcccgca acaatggcgg gatcagtcgc
    38161 tgtaatgatc aatgcaatta ttcgtgattt accgcaacag tttaacagta cctatgctga
    38221 cagtttagca gcagacaaag gaatctttgc cgtattgaat gtcatcatcg gaatcaatgg
    38281 ttttgtctgg aacggtacac tagcaatcgc cggattgatc tttgcctttt cttgggggta
    38341 taatcttgcc cgagcttatg gagtaaatga tctagctggg gggatcgttg gtttagctac
    38401 cttgattcaa ggtatttcct ttggtccagc attatcagca gcattagatg tgaatgttcc
    38461 ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt gcaacgattg cagatggcac
    38521 actgtcagtt ggattatggg gttggctaaa actagatcat ttgaatggaa acgcttattt
    38581 tacagttatg atcatgggag ctatttcagt cattattttt tgtaaattga tgcttgccaa
    38641 cattacgatc aaaatgcctg attcagtacc accagcagta tctaaagcat ttgcagccat
    38701 tttaccagcg acgatcgctt tatacgtcat tgctattatc aattatatcg taggtcgtgt
    38761 agctgatggg caactgatca ttgatttggt gcaaaaatat attgcagaac cattcctagg
    38821 attatctcaa gggttgggag cagtattgat cgtcacagta tttgttcaag tattctggtt
    38881 ctttggtatt catggaccaa atgtattggc accagtgcta gagggaatct ggggacaagc
    38941 acaactaatc aatattgata ttttccaaaa aggttataac ggaaaaactg gtacaccagc
    39001 cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg gtccgcggat cgttcgatgc
    39061 ttttgcatgg tttggcggat caggcggaac gatcgtctta ctgatagcta tcttgatttt
    39121 ctctaaacgt gcagattatc taacagtagc taaattatcc attgggcctg gtatcttcaa
    39181 tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg aatgcaatca tgtttatccc
    39241 attcttgatt gctccagtag tagcaacagc aattggatgg acagctactt atcttggcct
    39301 agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg ccgttcttat tgtcattctt
    39361 agcaacagga gcggattggc gggcaccgat cgtaacatta gtatgtatgg tcgtgacttt
    39421 cttgatttgg gcaccatttg tcatctctgc aaacaaaatg gaccctgccg caggcgaaca
    39481 acaataagat gaacgtgagg agcgggacgc tttaagtttc gttcctcata atgaattttc
    39541 gaggaggaac tgaaatgatt acaattgcca gtgcaatgca acaagatgaa atcaaagaat
    39601 tgttagaagg atacaatgaa gaaggaatga cttttacatt taaagaaaaa caaggaatca
    39661 agctgttctt tgaaacaagt gcagaagatc cggaagttgc ggctaaaaaa gcaaaggaac
    39721 taatcaaagc acagccttgg ggaacagtcc tttattttca atcaacagct gtttagaaaa
    39781 tggtttaaaa agataggatg gttgagatga taaaaaacgg tttattttta tgtagtattg
    39841 gttctttgat cgtgttgtat agtttgaatc cacattcgca aaggtatgat ttgtatagta
    39901 tggtcacagg tctttttcta acaggattag gaatctattt cttcatgaag gggaaaaaaa
    39961 gagaagaagc aaaggagaaa aataactaat ggaaatcacg aaaaaattaa atattccagc
    40021 atcgtttttt tacgataaag ttatggactc tgtccttttc gatgtacgta aagcgaccgg
    40081 caaatcagta actagaaaac agctgaaaaa tttggagtac gtcaaacaat tctcgcaaaa
    40141 taaccgggct cgcataaaaa tcgaagaagt cattgaaaac cagtcttata agtttcgtac
    40201 atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc aaaccattgg acgattcgac
    40261 ctgtgaggtt cattacacag aaaaaatgga gtcttatggc atgcttcaaa agttgaatga
    40321 tatgctttta ggaacaatct tgatgtattt taaacggaga cgtttcaaaa aaatgctaga
    40381 tatgatcgaa gcaacgtatt aaatcaatga aacatggtac tatttagtta ttgaaaaatg
    40441 aagtaagcta gaaggaattt ttcataggaa gatagatctt tcctgcgaat cctcactagc
    40501 ttttttgtag gaaggggcag caaaatgaca aacaaagtaa gagagctagg tgtatcttcg
    40561 attgaggaaa tgtttgattt agccacttat gcatttaatg gggcagatac acctgaaagg
    40621 cgagaacgtt ttcgtacact tgcggagaat tcttggaatt acggattttt tgatgaggag
    40681 gatcggctaa caagtcaagt catggcgacc ccttttccag tgaattttta cggacaagag
    40741 tacctaatgg ccggcattgg ctatgttgct tcttatccag aagcaagggg acaaggcggg
    40801 atcaatcaga tcatggaaaa gatccttgaa gattgccgtg atcgtaaagt aagcttatct
    40861 tatctggcgc cattctccta tcccttctat cggcgctatg gctatgaaca atcatttgat
    40921 aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta tcaaaaaaaa atctggaaaa
    40981 ataaagcgta tggattttga agatgcaaaa gctgcaatca gaagaattta tgaacagatg
    41041 ccggaaaacc aacgagctgg tctgaagcgc gaagactggt ggtatacgta taaattcaag
    41101 caaaaaggca attaccagtt tgctttgcat tttgatgata atgaggaagc aacgggatat
    41161 atcgtttatc agcttgcaac gtcttccttt attattgcgg agttgggatt tttggatcat
    41221 gatgcttttt gcagccttgt tcgcttcgta agttctcaca atggtgcttt tgaaacattt
    41281 gaatattcat gcggctacgg cggtaatgat caaaattatc tattagaaaa tccatttgct
    41341 tctgtaatga tcacacctta tatgatggcg aggatcgtag atatttcttt gtttttggaa
    41401 aattatccat ttaaaaagag acaagctgca tttgccctac aaatagaaga agatcattat
    41461 gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa atggaaaaaa tcatctctca
    41521 aaagtagaac aaacaaaact cccaatcgtt cgaggaagta tccaacatat cacgcagcta
    41581 ttattagggt atcgtaaagt ggaagagttg gtattccaag aaagaataca ggtgacaaaa
    41641 gaatatgaag aaacgttaag ctcaatcttg ccacagcaac agccaattct taatgactat
    41701 ttttgataaa aaacgggtga tgacaatatt tttgtcacca cccttttgtt tttcatttgg
    41761 catttgaagg aaaatgttaa agtaaatcct cttttttcgt aaatttgaat tttttgtttt
    41821 cttttgtttt agggttttct tttttgtggt cttggattgc ttctttcgta atcttttccc
    41881 gatttttttt agatgaatct aagcgttctg cgtatacttt ttcttttggc ttatcattca
    41941 ttggaaatga cctccctaca ttatttcatt ctacctctag tttatcatct gttaaggact
    42001 agtggaaacg ttttgcctaa cttataagct gattttttta gcagataagc ttgcaagtaa
    42061 aaaacaaaca agctacaatt gatttattga aattgttcct gtagctcagt aggatagagc
    42121 aatcgcctcc taagcgatag gtcgctggtt caattccagt caggaacgta caaataaccc
    42181 ctgtatcctt tgcgatacag gggtttgttt tgtgtttgtg ccaaaaatgt gccaaactag
    42241 ttatcatttt tatataaact atcgaacaca gcattgattt tttctttatc ctgatcttca
    42301 agctctcgaa caatatgaag ataagta
//
LOCUS       DAGCYL010000005        42192 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.005, whole genome shotgun sequence.
ACCESSION   DAGCYL010000005 DAGCYL010000000
VERSION     DAGCYL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.005"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(45..1613)
                     /locus_tag="MB216_000192"
     CDS             complement(45..1613)
                     /locus_tag="MB216_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBT4525056.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1617..2027)
                     /locus_tag="MB216_000193"
     CDS             complement(1617..2027)
                     /locus_tag="MB216_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBT4525057.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2024..2947)
                     /locus_tag="MB216_000194"
     CDS             complement(2024..2947)
                     /locus_tag="MB216_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBT4525058.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2944..3822)
                     /locus_tag="MB216_000195"
     CDS             complement(2944..3822)
                     /locus_tag="MB216_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBT4525059.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3809..5146)
                     /locus_tag="MB216_000196"
     CDS             complement(3809..5146)
                     /locus_tag="MB216_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBT4525060.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5149..6252)
                     /locus_tag="MB216_000197"
     CDS             complement(5149..6252)
                     /locus_tag="MB216_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBT4525061.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6326..7834)
                     /locus_tag="MB216_000198"
     CDS             complement(6326..7834)
                     /locus_tag="MB216_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBT4525062.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7800..8492)
                     /locus_tag="MB216_000199"
     CDS             complement(7800..8492)
                     /locus_tag="MB216_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBT4525063.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8514..9488)
                     /locus_tag="MB216_000200"
     CDS             complement(8514..9488)
                     /locus_tag="MB216_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4525064.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9788..10660
                     /locus_tag="MB216_000201"
     CDS             9788..10660
                     /locus_tag="MB216_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4525065.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11118..13316)
                     /locus_tag="MB216_000202"
     CDS             complement(11118..13316)
                     /locus_tag="MB216_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4525066.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13548..14540
                     /locus_tag="MB216_000203"
     CDS             13548..14540
                     /locus_tag="MB216_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4525067.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGVYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14600..16021
                     /locus_tag="MB216_000204"
     CDS             14600..16021
                     /locus_tag="MB216_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBT4525068.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16014..17513
                     /locus_tag="MB216_000205"
     CDS             16014..17513
                     /locus_tag="MB216_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBT4525069.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17552..18946
                     /gene="uxaC"
                     /locus_tag="MB216_000206"
     CDS             17552..18946
                     /gene="uxaC"
                     /locus_tag="MB216_000206"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBT4525070.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19105..19788
                     /locus_tag="MB216_000207"
     CDS             19105..19788
                     /locus_tag="MB216_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBT4525071.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19790..20626
                     /locus_tag="MB216_000208"
     CDS             19790..20626
                     /locus_tag="MB216_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBT4525072.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20637..22391
                     /locus_tag="MB216_000209"
     CDS             20637..22391
                     /locus_tag="MB216_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4525073.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22667..23008)
                     /locus_tag="MB216_000210"
     CDS             complement(22667..23008)
                     /locus_tag="MB216_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBT4525074.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23076..25247)
                     /locus_tag="MB216_000211"
     CDS             complement(23076..25247)
                     /locus_tag="MB216_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBT4525075.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25416..26315
                     /locus_tag="MB216_000212"
     CDS             25416..26315
                     /locus_tag="MB216_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBT4525076.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(26386..27243)
                     /locus_tag="MB216_000213"
     CDS             complement(26386..27243)
                     /locus_tag="MB216_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBT4525077.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27228..29930)
                     /locus_tag="MB216_000214"
     CDS             complement(27228..29930)
                     /locus_tag="MB216_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBT4525078.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(29943..31232)
                     /locus_tag="MB216_000215"
     CDS             complement(29943..31232)
                     /locus_tag="MB216_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBT4525079.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            31382..32428
                     /locus_tag="MB216_000216"
     CDS             31382..32428
                     /locus_tag="MB216_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4525080.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            32430..34583
                     /locus_tag="MB216_000217"
     CDS             32430..34583
                     /locus_tag="MB216_000217"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBT4525081.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(35056..36507)
                     /locus_tag="MB216_000218"
     CDS             complement(35056..36507)
                     /locus_tag="MB216_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4525082.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(36504..38228)
                     /locus_tag="MB216_000219"
     CDS             complement(36504..38228)
                     /locus_tag="MB216_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBT4525083.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(38221..38835)
                     /locus_tag="MB216_000220"
     CDS             complement(38221..38835)
                     /locus_tag="MB216_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBT4525084.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(39180..40637)
                     /locus_tag="MB216_000221"
     CDS             complement(39180..40637)
                     /locus_tag="MB216_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4525085.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(40678..41598)
                     /locus_tag="MB216_000222"
     CDS             complement(40678..41598)
                     /locus_tag="MB216_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4525086.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(41610..>42192)
                     /locus_tag="MB216_000223"
     CDS             complement(41610..>42192)
                     /locus_tag="MB216_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4525087.1"
                     /translation="TMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWY
                     NEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIP
                     QLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGM
                     TAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
BASE COUNT        12365 a         8642 c         7509 g        13676 t
ORIGIN      
        1 ctagtgaaaa taagttagtg aacctcattt attaaagaat taaattacag atgatctttt
       61 ccatcatact tgaaaaactc aaaatcagca taacttcctc tttttttatt tagatcatgc
      121 actgctatcc cgataaaatt tccagtaaac cctcctgaga gtatacacat gtttttttgc
      181 ggacctactt tcttccattc ttcagttgat acgtctttca aatgaaactg tgctgtctct
      241 ttctttacag tgactttcaa aaaagtcttc ttttctgtga gtggaatcgt ccaatcttct
      301 atttgtgcta ctccgttttc tgattctatc atacgtaggc attttccctt gtcctcattc
      361 caagtaacat aacaataaat ataattggat tcatttaagt acagtaccag tccagccatc
      421 tgattaaaat tattaggcat gtattcaaat gctgtctccg catcaaaagt aaaatccgtt
      481 tgccgaatag ctaacaaatg atgttcgaag gtagattgaa gagattcacc ggaaatcaga
      541 cgtaaaaaac ctggtctaca ttttgtatca caccaatcag catttggcaa aatgcgtctg
      601 gcattccagt tcttctttag ctctccttca aatgtatcca taaactgatg atctttttct
      661 tgttgtacct cttcatttgt ctcaatcatt atttcttcca tcggagttgt ctggctatct
      721 ttaagtctta accaatcgtc ggacgtccaa tacacttctt gaatagcggt ttctcttccc
      781 aaaatagccg caccgtttaa tggtctggta caaagatatg tcatgtacca tcttcctgac
      841 ggggtttgta ctaagctccc atgtcctgta cattgcaatg tagaagcagg tttgtcacta
      901 gctgtcagca taggaaaacc aggatcaagc tcgtaaggac cagtaatatt ctttgctcgg
      961 caaactgtaa ctgaatgtcc tcttcctgta ccgccttctg ccgtcatcaa ataataatac
     1021 ccgttacgaa aatacaaatg tggtgcctcc gttttcgcta attctgtacc tgcaaatatt
     1081 ttatagatgg gaccatcaag cttcttcttc tctgcatcat attcttgtaa tacgataccg
     1141 ctggatttat tcggtgtgtc cagacgataa tcccatagtt cattgacaag ccattttttt
     1201 cccgtttcaa gatcatggaa caaagaagga tcaaagccgt tactatttaa ataaattggt
     1261 tcggaccaag gacctgtgat ctcagatgca gtgatcaaat aattgtggac atctttaaac
     1321 ggacggcctg cacttttgac atctgtgtaa acaagataaa acaatccatc agcataactg
     1381 atctgtggcg cccatatact tccatttgta ggatttcctt gcaaagatac ttgagtggtc
     1441 aaaatatccg ttgtatgtga ccagttgact aaatttttcg actcatatac tcggattcct
     1501 ggaagccatt caaaagagga gacaataatg taataccgct ctttcactct taatatatta
     1561 ggatctggat tgaatcctct taaaatagga tttttcactt tgatagtctg catgattcag
     1621 gctccttttc tcattcttac tcgatataaa ctgaatgccc cagctgcgaa ataaaaaaat
     1681 gcaaaaggaa acgcaataaa ctgagtgcca atcaacaata tgaatcctgt tataaaatac
     1741 aaaccagtgg cagttgttaa gtattttcct ttagcagtca aaaacaatgc tactgctaaa
     1801 aaagcaagtg aaataaacaa cacaacggca aacagaccag tcatgtcctg taataatcca
     1861 aatgtatttt ctaaagacaa agttgaatga tctacagcaa gcgactgata acttttttta
     1921 aactctgacg cagacatatt acttaaagta actgctaacc cgccaaagaa aatcaaagat
     1981 agactgccac cgaccattcc tagcaatagt tcccattttc ttttcatttc tgaaccccct
     2041 gcttttctaa atgtatccat tcaggtcttt tgatcgatgt ttcataattg ttattacgtt
     2101 cagagaattt gacagtctgt tcatttttct tgtcatccca attatcccaa ccagttgggt
     2161 gaacatgccc acctatctta cacgcttcaa ataagacatt tgcatacgga cgccacggtc
     2221 ttcctaaata gacgtttcta gtatcaggtt ctgcagtcaa ggtacagctt tcaaaacgat
     2281 accctgtttt ttgatttttc ggcgtagatg ctgctgtgat gtatccagga tttttactgg
     2341 ctaagctatg gatatgacac tgatgaaaaa cggcatctgc cccaccaaat atgaaatcca
     2401 ccgttccttc aatccagcaa tattcatagg tctgacgaca atactcagga ttggcagaag
     2461 ccggtgtaca aaaaggagta ccatcttttt gcaaatcagg caacggaccc gtgcataagg
     2521 tatcctgata accagatagt cgacagtttt ttaaagatac gcgatctcct gaattgaata
     2581 aagcaacagc ctgtcctacc ttttctcctg gacctgctgt attttcgatg tgaagatttt
     2641 ctaatacgac attcgatcct tctaagaaaa aagtagcact tcggaaagtc cctaactggc
     2701 taccgtcaag atactcctgt tttgcaaaac gattaccgga gataactact gttcctgctc
     2761 ccacaagttt aaaattggat aatctgcttt ctatatattc atcatatctt ccttctttta
     2821 ctatcaagac ctttggatgg ctatccggca ggcctgccaa ataatcgatt ccttcttgga
     2881 tagtttcaaa atccgtcggg gtatctatag cattctttcc aattatcagt ttgtctttca
     2941 tcctcataaa tgctccctta tgaattgttt ccaaatattt ctcatttctt gtgtttccat
     3001 atgaccgcct gttaaagaaa aagaagaaaa gttttctgga ctacccattg attgatactt
     3061 tttgtccagc ccttttctca gcttcagtac gccttcgtcc aagcacatcg tatcgttttt
     3121 tccaactaag ctaagacgag ggcgcggagc gattttttct tggattgcta acgttgtata
     3181 agctgtcaat agatttggca catagtaata aaacccatga tgatctaggc ggcgatgtag
     3241 caaaagtatt tcgatgtcca cctgtgccgc tagatcaata caaactttga cacgagaatc
     3301 caatgctgtc acccaccagc ttaataagcc tcccatagaa agaccaatcg ttgcgagccg
     3361 ttgagtatct gtgtcttccc ttgtctccaa ataatcgatc aagctgatca catcataaat
     3421 acgcattccc cataacgttt ttcctgttaa taaaaattgt ttgaacaact cgctttcaga
     3481 aatacctccg cgttcttcaa atccccaagc atcaatcgac caaacagcat atcccattcc
     3541 tgtcagtact tctgcaaaag atggagaagc taagtaagga gcaccatgga tcaattcact
     3601 tttcccgact gaaaaatctc cgccgtgaga atgcaggtaa aggaccggcg gaaacggtcc
     3661 ttctttttct ggataagcat agagggctgg tactgtttcc aattcattta agtacaattg
     3721 atagtattca agcataaaac cgttttgttt ttctcggtat aatcgttttc cactaggggt
     3781 atgtctgcta gatggagcac ctaaaaaatc attcaactgc ataagcaaag tttttacgaa
     3841 caaccagttc atccatattt tctgattgat ttgattggaa tttctcaata ctgatttctt
     3901 tagtattttc taatagaaat gctggtccct cgtgattttc aatcgacatc tggttaaagg
     3961 aaaccttacc cgcaaagcca atataaaatc cttgtctgct catttcctct attcctgtca
     4021 tcatagcagg aacaccggga actgcatctt gggccatcga gacatcgata ttgtcaaatg
     4081 tgatttcttg tatgaattgt tccgctaatc cataaataaa acctgcagaa gcatggacat
     4141 tccttgccgt gatattcgaa aaatggatcc ttctgaatgc tggtgtccgt tcatctatcg
     4201 gataagcctt tttttcccat acatacggtt ctttccctcg cggaccacag aaataataaa
     4261 gattcagtat aaatggacac atcacattat ccatgactat attggatact cgaatatctt
     4321 caacgatccc gcctcgacct cgtctagatt tcaatcgtat tcctcgatca gtttcttgga
     4381 agatacaatt tgaaatggta atattacgaa tactaccact catttcactt cctaatacga
     4441 caccgccatg tccatggacc atcgtgcaat ttgtgatcgt gatattttcg caagcgatcc
     4501 tttcgtacgt atcttcagtc cctgccttga ttgcaataca gtcatctccc acgtcaatat
     4561 ggcaattact aatacgcaca tttttgcatg attctggatc aattccgtct gtatttggtg
     4621 aatccgctgg gttcaagatc gtcaagttat caaacgttgc attactgcaa agaatcgggt
     4681 tgatcgtcca acttggtgac tggatcaatt taatatcttt cacagtaatt cgatgacagt
     4741 tatggaaact catcaattta ggacgaggat aagcgagatt atctggttca tttctaaaag
     4801 tatgccacca tttttttcca tttccatcta atgtaccgaa accagtgaca gagatgttct
     4861 ctacattttg agcatagata caggaagcat aaacttttcg atggactcct tcccaacgtg
     4921 aatgaacaac aggataatct tttggatcgt cagagaattt cagaaccgct cctgcgctta
     4981 aatgaagttc gacattgctt tttaaaaata aagctcctgt tagaaattca cctgctggaa
     5041 caactacggt cccgcctccg tcacttgctg cagcatcaat ggcttgttgt attgcttcag
     5101 tattcaattc atcaattgat gcgccaaatg tcaaaatatc atacatattt ataatgcctc
     5161 cacttcaatc aatgcttgca agaatgcacc aacgcctttt tgatcattac aaataattgg
     5221 ttcactgatg tagtaagcat atgaaccatc tcgctgatct gctccgccta accctgctac
     5281 ttgacagttt ttattcaagt tcagccagcc ttcttttgtt tccagaacaa attcatcaag
     5341 taatccttga tgtgatttct tcacttgtgg aagccattct tctttgttca gcagtcccaa
     5401 acgtattcct ttagccattg cgtaaacaaa catactactg ccggaagctt ccagataatt
     5461 tcctttccgt tttgtttctg ttgttacttg ataccatgtc ccgctgtttt cttcttgata
     5521 tggaagtaat gcagccagtg tcttttctag aatttctttc agcaaagaac aatctgtttc
     5581 ttcatgaagt aattcatagg tatctgctgc agccatcaaa taccagccaa tcgatcggct
     5641 ccaaaaatga tgggatagac ctgtttttgg atcagcccat ggttggacct tcttttcatc
     5701 ccatgcatga atcagcaaat ttgtttttgg atcgaataga tgtttataac tgatttcaaa
     5761 ctgtctcaaa acatcttggt aatctttttc ttcactaaaa ttctggataa attcagcata
     5821 aaaaggagaa cccatatata agccatccaa ccaaatttga taaggataaa tttctttatg
     5881 ccaaaaagct ccttcggatg tgcgtggatg gtttgccaac tgctgggaaa ggagttctgc
     5941 tgctttttta tatttttctt cgcctgtttc tttgtacaat aaaaagaaaa gctttcctgt
     6001 atttacatgg tcgatattgt attcttcaac gctgtagcca cggatcgttc catcttcttg
     6061 cacgaaatag tcgatattgt cttttatgaa atcaaaataa attttttctt gggttcgttc
     6121 ccaaagtaat tggaaccctt taagtacgac gccatagtca taagaccatt ttcctttata
     6181 ccacttttct tcatataagc gaggtgttct ttccatgata ctttttgcta atttctttga
     6241 ccagttttcc attatttgct ctcctttaaa aattaaaata ggatttggga caaataccaa
     6301 gaagtatttg tcccaatcgt ctatattact gtgccgctgc tttgtatgct tcctcgtact
     6361 cttgtgtgat tcgatcgcct ccttgtgctt tccagtcttc tacagctttt ttgaatccat
     6421 cttcgtcaag ctgtcccatg atgtattgca ccgtagcatc aatgacgatt ttttcaattt
     6481 ctgatcctac ttcattgttc gtcggtgaat ccaatggcac agaaggatcc attacagcaa
     6541 attcttcatt ttctttgatc aattgattag agaattcttt ttcagggtct gtatctttga
     6601 aagagaacgt cacttcacta ggacgagagc ttgataacgg ttgcacctct tgttgccaaa
     6661 gatccatatc gataaattcg atagaaccat catcattcaa ttcataatgc gttccttcga
     6721 taccgccagt catcaattta tacacatctt cttctaaaag atcatcaaca aattgaagta
     6781 atttcttcaa ttgtgcttcg tcttttactt gagatttggg aaatgctagc aagccgccaa
     6841 taccatttcc ttctgaccaa acatgataac cgccgccttc accatctgag atcttgttta
     6901 caggcactaa ttccatatca tcttgaatac cttgacccat cgtcttcaga ttcttgatat
     6961 ccaccattcc agtgtagatt ccagttcttc cttgtgcaaa ttgttcttgc tgatcagtct
     7021 tttgggtaac tgcaaagtct tgtgccaaat agccattctc aaatagatct cttgaaaaat
     7081 tcaaagcttt catatactct tctgtatcaa attccggagt gaatttgcca ttatcgtcta
     7141 cagcccagcc atttggtgct ccaaaataag aagtgaaaag tttaaagctg gtataacgaa
     7201 tatcactagg cgcacgatcg ccaaaaccaa ctgtgtcgtc cacaccattt ccatcaggat
     7261 cgttttctgt aaattgttta gctacttcat aaagctcatc catggtagtt ggaacatcta
     7321 gtcctagatt atccaaccag tcttttcgaa tgactaagcc agcacgagcg tagtcttttt
     7381 gtatcggtac gccgtacaag gcaccgtcga tactcgcagc ttcgattcta tcttcagaaa
     7441 tttgacttaa atttttatag tcttttaaat attctgatac atcccagaat acgcctgatt
     7501 ttaacgcatt gcggacagaa gagtttttca acatcgcaat ttgaagtgta acgatatctc
     7561 ctagttcatc agaagccaat gcagttgtta atctttcttc ttttgaagca tcaggaatcc
     7621 agttgaatgt cagttccgta tcggtttttt cttcgatcaa gttcacaatc gtgtctgtag
     7681 gcggtgaagc atgatgcaaa atatttaacc aagtgatttt agcttttcca tcttctgtct
     7741 ctgaagaact gttacttgaa ctgctgccgc atgcggcaag tgctagaatt cctgcaaaac
     7801 taagacctaa ttttacgagc gttgcttttt tcataaattt tctccttcac tgttttaata
     7861 atatttttgt tttcttctgt tttttctgta tatgagttct tccattccaa atggcaaaat
     7921 atagaatttg ctgtccacgt gactgcggaa aagaagagac tgccaccgat aaaggttaaa
     7981 acacctggca ttgtttggat gatcatcaga taaatcccta ccatacttaa tatcaacact
     8041 aatgagtaat gaagatgtga gaatccgaat aaaaatgcca ttttcatttt gaaaaaatat
     8101 cctttccaat cataatgagc catgattggg aaaatataga gtgctgagag cacatatatg
     8161 aacgcagcaa tatacaaagc gatcctaaga taccaattgt gtatgaaaga aagatccaca
     8221 agtaaaacaa tcccgatggc gccatatatc cagctcatca gcaaggtttc acgatagttt
     8281 tctttaaaat atgtgaagta cgttttcgtc aaaggaaacg tttcttttga gcgcagccat
     8341 tttctaatca ctgcaaccat cgcatacgat gcaggaccga aagtaaaaac tcccaaacct
     8401 gcgatgacta aaccgcccca cagaaaattc aaataaacca actgtaaaat acgatttagc
     8461 cttccgttta ttttatctaa tgactgcagc atatctttcc ctctctttct atcttagtaa
     8521 acgccgtctt ctcctagctt tttggcaatc ttattagcta aaattaccat aaacaatcct
     8581 acgacccctt taaacaatcc cattgctgta ctgaaactta actgtccatt cttcaatcca
     8641 gctgtataaa tataggtgtc gaaaatttct gcaacgcttc gattcaatga attcaataac
     8701 aagtacatat gctcgaaccc taagtctaat gtctgaccga tttttagaat cagcaaggta
     8761 atgattactg gacgaattgc cggcaatgta acatgccatg ttttgcgtaa gcgtcctgct
     8821 ccatccattt ctgcagcttc atacaactgt gtatcgactg cagttatcgc tgaaaggtag
     8881 atgatcgtag accagcccaa ctctttccag atcacttgtc cgatatatac tgtgcgtgtc
     8941 cattcagggg atgttagaaa actgattttt tcaaatccca ttgttgcaag gaattcattc
     9001 aatacccctc cgtcaacatt caaaaataca taagtaatcg aaacaatgat tacccaagac
     9061 atgaagtgag ggatgtaaat gatcgtttga atcccgtttt tcaatcgttt gttcgttact
     9121 tcatttaata gtaaagacaa aatgattggc atcggaaaaa acacgaagat atttaatccg
     9181 aacaagatca acgtatttct taataatgta aagaatgtcg gttctgtaaa caaacgaatg
     9241 aaatgtttga aaccgaccca aggacttccc attattccta aaaatggttg ataatcttga
     9301 aaagcaataa cgagtccccc cataggaaga tacctaaaaa tcaagaaata aaggactccc
     9361 ggcagaatca tcacatataa aaatttattc gttttgattc ctgcaattat tttttttcgt
     9421 cttctgagct tttcttcttt tttcgatagc gtcagttgaa atggttcttg tttctttaca
     9481 ctttccataa gcccctcctt agttttgtga atgtttgctg cttatgactt catgatagat
     9541 aactatctga attacgtata taatcatttt taccgtaagt aaaaacgctt acatagcaat
     9601 gatttttgtg ctatacaaaa cgataaagtg tattttttca caaataaact acgcatagca
     9661 aaccttctat ttttttctat ctgacactta gcataggaaa tgattatata cgtaatcttt
     9721 caccgatgat aaattgaaca tatagaaaca gaaacattta atttattttg aggaggagcc
     9781 tatctatatg aaaaagaaag tctctatagg agaaagaagt tttacacttt tcaacaatac
     9841 gtttctaatt ttgctagctt tgatctgtat cgttcctttt ttaaatatca tcgctacatc
     9901 ttttgcttct acacaagaag tcgtcgcgaa aaagtttatc ctttttccta ctaccttttc
     9961 tttagacgcg tatcgctaca ttctttctac gccgaccatt tttagagcac ttgctgtttc
    10021 gattggtgtt acaggtgtcg gaacgatcgt cagtatgtgt gcaacttcac tgatggccta
    10081 cggtctttcc agaaagtatt tgtttggtcg aggtttcgtc aactttcttg ttgtattttc
    10141 tatgttgttc agcgggggga tgattccaac gttcttagtt gttcgttctc tagggctggt
    10201 caattcttac tgggccatga tcctgcctgt ggctgttaat gcaatgaata tgatcattat
    10261 gagaaacttt ttccaagcct tgccggatag tttggaagaa tcagcaaaaa tggatggttg
    10321 cacagatttt ggtgtatttt tcaaaattat gctgccatta gcgttaccgt ctattgctac
    10381 tatctcgctt ttttatgctg ttacttactg gaacacttat atgactgcta ttttgtatat
    10441 caacgattca tctaaatggc cgatacagat tcttttacgc caaatcgtca ttgtatcaag
    10501 tgggatgcaa gcagaaagta gtgccgttga tgtgattcca ccagcacaaa cagttaaaat
    10561 ggctgttatc gttattgcaa cagtgcctat gctgattgtt tatccttttg ttcagaaata
    10621 ttttgtcaaa ggagcactcg taggttctgt caaagggtga ttatgtactg aacgcaaaat
    10681 ggtagttgtg tcagaagtgg tcagcttcaa gaaataagaa ggcatttacg aaaattgctt
    10741 cttaaatttt tgtgaaattt cggcttattt ttcgaagaat ctactcctgg aacaccgtgt
    10801 atcagaggtt gtgaaagaga cgttcagcct tgagcgatga taaaaactct cacaaacatt
    10861 gcgacgagta acccacagga gcaataagaa gcgattttga aaaatagctt cccatatttc
    10921 acaaaattat gccttattgt cgatagcctg tctcttgaac gccatttatt cgtaaaaaag
    10981 aactgtgaca agacttttgt cacagttctt tttgcgtgct tagccttgaa atttaactat
    11041 ccataaatac aaatactagg gttattctaa cttcttttat tgcttgaatc agtccacaag
    11101 aaaaacagcc tcttgattca caaatatttt tttcgatatt ctcccggcgt aatcgattcc
    11161 ttctttttaa agaaacgaat aaaattttgc ggatttcgat attgcaatct ttctgcaatc
    11221 tcttttacgg agaggttagt ctctttcaac catttttttg ctacctctaa acgatgattt
    11281 tgaacaaaat caccaaattt ttctcctgtt tcctttttaa atacattact taaatagtta
    11341 gggttataat gcaacctatc agcgatcaca tctaaagaca gatcacgatc atattctgtg
    11401 tagacgatat gcataatatt ctccgacaaa tttttaaatt catgatcagt gattgcttga
    11461 gagcattctg tgattggcag aactaattgt tctaaaacaa gtttttctag ttctttggga
    11521 tgataattgt tcagtgcttt tacataaatt tgtttcatgt tatcaaagtt ttttgaatct
    11581 actcccaata actgtcctaa ttgaacgagt tcattgatca agcggaccga aactacttcc
    11641 cgactcaaag gatttttatt ttgtgcaaac aatgcatcaa tcaattcatg tactaaattc
    11701 ttcacttcct gatttccaga acgaatggct tcaaacaatc gattttgttc ttctatcgga
    11761 tatctaataa gtgttttttc atgagcagca ggaacgattt cctgataaaa aataattgaa
    11821 cttggaccag tatttacacg ataatacagg gcttcttttg cccgatcaac cgattgcttg
    11881 ctttcattca gcgtttcgaa ccggttactg atgcctatac tgatagttaa attcaagtat
    11941 tcttttacac gtttttgaat gagtcggcaa tactccatga cttttttgct tgcttctgca
    12001 tcatctttat ccattcgata aatagtggct tgcatttctt cattcaaaac gatcggcagc
    12061 attcgatttt cctttggcac aatttccgtg atcatgttgt tgatcgctaa aagtagcaaa
    12121 tcacgctccc caccgtgact atcttctaac agatcgattt gaaccaaagc tgtataatag
    12181 cagtcatttt gagaactata accaaattgc tgcaaccgct gatctagttc tcgcctttct
    12241 actcgatttc taaataaact aagtacaaat agcgtttcca gctgtggctt ttgggtaact
    12301 aagtgtgcac ttaacgcttc tttttctcct atgatccgac tgactgattg agcgaccaaa
    12361 tctagttctt tcccactgaa tccttttgat ttaagattca gacgttcctg tatttgagaa
    12421 atcggacggg caaatctgtc cgaaaataag taggaaagaa gaccaatgag tcctaccagt
    12481 gttaaagaca ctgcaactag cccgattctc agattgcgga tcattgcgcc aattgcacta
    12541 gagtcaactt ctaatctgta aatccaatta ttataatctg atttcagtac tatatacgtt
    12601 ttatcttttt ctcttatcac tttcagctga ttgtaatcta gatccacgtc cgttaataca
    12661 gttggttgct ctttattttt tactgtctga gtagtaaaaa ggttgccaac ttcgttttca
    12721 atagaaagta tgtcttctcc actttcacca acaacttgtt ctattgtatg tttctctatc
    12781 gtagcaattc ccaatgcata tttttctttt tcaaacattg gcaaagaaat caccatttcg
    12841 attcctttgt ttttcttttg ccaaaataaa ttatttttgt tatttatgta cttctgttga
    12901 tacttttcaa tttcctcttc ggtcagttga ctcaacgatc cattgattac tccccattta
    12961 ccagccaaac tgactaaatc ataattatta tcatccataa caatcgtttc tataaaattt
    13021 agttctttcc tgatttcaga ataagtggag taatctgtat atgtcagcgg atgcttgaat
    13081 atgtccgaga aatttttggt cgaaccatac gtagaaaacg aatattcaat cgttctaatt
    13141 ttccattcaa cgttagtttt aacttgcctg agcaaattct ttttgtctgt taaataactc
    13201 gattcgatcg attcataggt attctggtaa atgaataaac taaatcctat gacaagtacg
    13261 gctcctaata aaaacatcgg taaaaatatt ttataaaata ctgtactttt cttcattttc
    13321 ttcctctcct ccttttaaaa taaaaggctt tcatttttct gtaaaattga tactcatcta
    13381 attaaatgag cattccccgc tttcaagaat agcataatta aaaataaaaa tccaagtaat
    13441 ttttaacgtg tgcaatcttt tcatatcaaa gatctattga cttatcgtag ttatcgtaat
    13501 atgatgaatg cgttaacaaa aagtgaaaaa gtaaggaaaa tgatggaatg gtaacgatca
    13561 aagatgtcgc taaagcagct ggcgtttctc atacgacagt ttctcgtgct ttgaataaca
    13621 gcagtttaat aaaaaatgaa acaaaacaaa aaatacgaca actagctaag gaaatgaatt
    13681 attcgccaaa ctatagtgca aaaggtttag tgaatcaaaa aaaatatcta atcggactat
    13741 ttttctcaag tttgcataaa ggaacttcat caaatttcct agcagattcc attgcaggca
    13801 tccattcagt attagacaca aattacagtt tatctgttga aagtatcgat gttgttgatt
    13861 taacagaaat caacctgcaa cgctacgatg gactgatcgt tatgagtcaa agtgatgatg
    13921 atcagccttt catcgactat ctcaaagaaa aagaatttcc tttcgttgtt gtcaatcgtc
    13981 acatcaatga tccagcggtt atcaacgtca ctgccgatga tgcggccggt gttatgcagg
    14041 cagtagatta cggtattgga tgcggacata cgaagatcgc ttatattggc ggaaagaaaa
    14101 attttcgttc gacaaaagaa cgggaaaaag gggtattgaa aagtctaaaa aaagcgagca
    14161 tccctgtcaa tccagcctat tttttaacag gagtttacag cttagaaagc ggcctccaaa
    14221 acatggaata cctattatca ctttctgacc tgcctactct ggttttttgc ggcaacgacg
    14281 acatagcgat tggtgcattg agagctgtac atttagctgg cctccgtgtt ccccagaaca
    14341 tttctctgat cgggttcgat gattctccag tcgtctccta tctcaatccg ccgcttacca
    14401 ccgtacgtaa gcctttgaaa gaaatgtgtc aagaaggaac aaagctttta ctagatttga
    14461 tcgaaggcag gacgatcgaa caatctaaaa ttgagctctc caccttgttg atccttcgtg
    14521 agtcgatcaa ttacttgtaa actacaagta attttatata aaaatgttaa cgtgtgcata
    14581 acacagaaag gaagtttata tgaaaaaact gacaaaagaa ttagtgaaaa aaaacgaagc
    14641 acctgtaaaa gttcttcagt ttggtgaagg caattttatg cgtggattta ttgattggca
    14701 gattcagcag ctgaataacc aacaattgtt taaaggtaat gttgcaatca ttcaaccttt
    14761 agaacaaggt cttggaaata tgatgaacca gcaagataat ttatatacgg tcattttaca
    14821 aggactcagc aatggcgaaa tcgttgatac ttccgagatt atcaccgtag tagaacaaat
    14881 gatcaatcct tatgatgaat gggatgctta tctatctcta gcagaaaatg acgacttggc
    14941 ctatatcgtc tccaatacaa cagaggcagg aatccaattc gtggagactg ataatttaac
    15001 agatactccc ccacagagcc tcccgggcaa actgacagcc tttttatacc gacgtttcaa
    15061 attaaacaaa gctggcttta ccatcattcc ttgcgaactg attgacagaa acggcgaacg
    15121 tctaaaagaa atcattttac gctacgcaga aaaatgggat ttagaacctg cttttgttga
    15181 gtggttagaa aaagaaaatg ttttctgttg tagtttagtt gatcgcatcg tcccaggtta
    15241 tcctcgcgaa gatgcaggtg ttttcaatca acggcataac tatgaagatc aattgatggt
    15301 caaagcagaa ccattcatgc tttgggtgat cgaagggcct aaagaattgg agaatcaact
    15361 accattcaaa caggctggct tgaatgtcat catcacagat gatatgactc cttatcgtga
    15421 acgtaaggtg catttgctta atggaccgca tacagcgatg gtccctcttg ctttgttggc
    15481 ggaattggaa actgtagaag aagtaatgaa agatccttta ttctccgcat atgttgatca
    15541 gttattcaat ctagaattga ttcctatgct ttctttacct aaagatgaat tagccattta
    15601 tgctgaccaa atcaaagaac gtttcttgaa tccttttgcc catcacaaat tagaagcgat
    15661 ttcattaaat agtgtttcaa agttcagcac ccgtctgctt ccagtgttca agaagtatat
    15721 cgaagaacaa aatcaggtac caccgttaat cactgtttca ctagctgcgc tacttcttat
    15781 gtatcgaggc gatcaagtaa agccacatga tgatgaaaag acgataagcg aatttactga
    15841 tgcttggtct gataatggaa cagctattcc tcgattattg caaaacgctg cgctttgggg
    15901 agaagatttg tctcaaatac cgaatgtgac agatacagtt caagaaatag ctgcaaagat
    15961 cgatcaaaaa ggtgtccggt cagtattaca agaaatgaaa ggaacgatgg tccatgcttg
    16021 aaaccctgca accaacaatg aaattaaatc gacgagacag tgtggtcgtc gcattaaccc
    16081 ctattccaaa acaaacacaa ctgacgattg atcagcaaac gatcaccact cttgaagata
    16141 tcccgcaagg tcataagatt gcattgggtg atttgaaagc tggagacaac gtgatcaaat
    16201 atggctatcc aatcggtcat gtgacgacag acgtaactgc cggtcaatgg ctgcacactc
    16261 ataatgtaaa aaccaatctt tctggtgaat tggattatcg atatgaaaaa gatgtccatc
    16321 caagtcatta cctttttgag aaccgaacat ttcaaggtta cttgcgaaaa aatggaaaag
    16381 tagggattcg taatgatttg tttttagtac ctaccgttgg gtgtgtgaat ggcattgctg
    16441 aactgatcgt caaacagttc aaggaaaaac atccagatct gggacagttt gaccatatta
    16501 cgattttaaa gcatccttat ggctgttcgc agcttggaaa agatcatcaa aatactcgag
    16561 aaattttagc ggacgcagta aatcatccca atgccggagg agttttagta tttggattag
    16621 gttgtgagaa caatacagtt cctgaattca aaaaaatttt aggtgaatat gatgaagaac
    16681 gagtgaaatt tctagtggct caagatgttt acgatgagat cgaacaaggt gtggctctat
    16741 tagaagagct tttaacagcg gcggaaaatg accagcgcat atcagtcccg ctctctaagt
    16801 tgaatgtcgg tttgaaatgc ggcggttctg atggtctatc gggaataact gctaatcctt
    16861 tacttggtgc attttctgac tacttgattg ctcaaggagg cagcacgatc ttaacagaag
    16921 tgccagaaat gtttggcgca gaacaagtat tgatggcacg agcggaaaat gaagaagttt
    16981 ttgactcaat cgttgacttg atcaatgact tcaaacagta tttcttgtct tacggggaac
    17041 cagtttacga aaacccatct cctggtaata aagcaggtgg gatcactaca ttagaagata
    17101 agtctttagg ctgcacgcaa aaatcaggaa gctctcctgt cgttgatgtg ctacaatatg
    17161 gcgaaaaaat cagaaaacca gggcttagtt tattgcaagc tcccgggaat gatcttgtag
    17221 ctgcctctgc tcttgcttca tccgattgcc agttggtctt gtttaccaca ggaagaggga
    17281 ctccgtttgg ctcatatgtc ccaacggtca aggtatctac gaatacaact ctttttgaac
    17341 gcaaaggtca ttggatggat tttaatgcag gcgtcttatt agaacaacca atggaagtaa
    17401 tcttagaatc gtttgttcaa aaaatcatcg ctgtagctag tggagaagaa acgaaaaatg
    17461 aacaaaatga cgttcgagaa attgccattt ttaaaaacgg agtaacactt tgattttcat
    17521 aaaacagtat aaaaagaaag aaggaagatt tatgttttta tctgatgatt ttttattaaa
    17581 agacgattgg gcgaaaaagc tttaccattc ctacgcaaaa aacatgccca ttattgatta
    17641 tcactgtcac ttagatccaa aagaaattta cgaaaacaac aatttcagca atctgacaga
    17701 agcttggtta ggaggcgatc attacaaatg gcgcttgatg cgagcttgcg gcgtgcctga
    17761 agaaaaaatc acaggaaatg cttctgattt tgaaaaattt ttagcttggt gtcaaaccgt
    17821 accaaaaatt atcggaaatc cgctttacag ttggtcacat cttgaattga agcgtttctt
    17881 cggtattgac ttgttgataa atgaagaaaa tgcagagaaa atttggacac aagcaaatga
    17941 aattttggca gctcctgatt ttcgccgtcg ggaaatcgca aaaaaatcga atgtagaagt
    18001 gatttgtaca acagatgatc ccgcagacga cttgcactat catcagttgc ttgctcatga
    18061 agaaccagat ttgaaggttc taccttcatt ccgtccagat aaagcactca atattgaaaa
    18121 agaaggattt aatgcatggg tcacactact ggaagaagca gctgaccaaa cgatcaccac
    18181 ctatcaagaa ctgatagaag tcttagggca gcggatagaa tacttccatc aacatggatg
    18241 tcgtgtttcc gaccacgctt tagacatctt acgctataaa gaagcggatg aaacagtatt
    18301 agaagagatt tttcaaaaag cacgagctaa caaactatta tcttcagatg aaatcgatgc
    18361 ttatcgtaca gaaacactga tacatttgac ccacttctat cattctcata attggacgat
    18421 gcagctgcac atccatgctt atcgtaactg taatacacaa atgttggaaa aattagggcc
    18481 cgataccggc tatgatggaa tgaatgatct ttctttgact attcctctgc aaaaattatt
    18541 gaaccgtgca gaagaaacgg atcagttgcc aaagacgatc ctttattcat tgaatccgaa
    18601 tgactatcct gcgattttag ctttgatggg ctgtttccaa aaagaacaga atggaaaact
    18661 gcagttaggt tctggatggt ggtacaacga tactcgagct ggtatgcgcg atcaattgac
    18721 acaatttgct gatggtagtg ctttggggaa ttttgtcggc atgctgacag attcaagaag
    18781 tttcctgtcc tacactagac atgaatattt ccgtcgtgtt ctttgtgaat ttttaggcga
    18841 aatggtcatg cgaggagaag cacctgaaga tgaacaactt ttaggaagtc tagttcaagc
    18901 gattagttat acaaatgcta aaaattactt tggctttttt gcctaattga ttaatagtaa
    18961 cagtaacgaa tcaaaaacag gatagtgaaa taacggaatc cgtttatcca cgttttagag
    19021 aagaagacgg accaaatttg ctccgtcttc ttctcttgct gttctgcttc attgcctata
    19081 aacctttatg aaagaagaga taacatgcct actatccata ttgccggtga ttctactgcg
    19141 gcagaaaaag atgaaaacaa gcgtcccgag acaggttggg gagaaaaaat ctctgcttat
    19201 ttttcacccg agatccagat caataaccaa gcaaagaatg gacgcagcac caaatcattt
    19261 atccaggaag gaaggttagc ccgtctagct tcacagtttc agccgaatga tttcttattt
    19321 gtacaatttg gacataacga ccagaaaatc caagaagaaa gaggtacgct tcccttcggt
    19381 agctatttaa aaaatctgca gatatttttt gaatgcgcaa aaaaagcgaa cgtccaaatg
    19441 gtcttactga catctgtgac gcgacgagat tacttagaaa acggaacatt gaatccagat
    19501 atacttggag actatccaaa agcgatgcgt gcatttgctg agaaacacca tattcctctt
    19561 ttagatgtgt tctcaagatc acaagaattg ttccagacct tctcaaaaga agagacaaaa
    19621 aaattctacc tgcatcttat acctgatact tgtaaaaatt acccagaagg actaaaagac
    19681 aatacccact ttagtccaga aggcgctgat aaagttgccc gaataattgt tgaattgata
    19741 aaagaatccc gtctgcctct tgctaattat ttacaaaaag gagtttgaga tgtttccatt
    19801 aaatttaggc atccgtgccc acgatttaga tgttcgaaat cgtgaagaaa tcgttcaaaa
    19861 agtatcacag cttcacttgt cccatatcca atttgcacca caaaaagcat ttcctgaaca
    19921 acttaaatta actgacatga cacttgggag tgctgcctat tttggtgaat acttccaaaa
    19981 aaacaatatc gaactttcca ttttaggatg ctatatcaac ctatccagca aaaatcaaga
    20041 agttcgtgca caagcattgg caactttcaa acagcaaatt gctctgtgct ctgcctatca
    20101 agccaagatg attgctacgg aaaccggtag tgtctctgtc ggttacacaa aagaaaattt
    20161 tactgaagaa gcttatcaga tcgccagaaa ttctatcatt gaattagtcg ctcatgcaga
    20221 aaattttggc gtaactgttg ctgttgaagc agggatcaat catccaatct ataactatca
    20281 gttagctaaa cgtttaatcg atgaagtagc ttcaccaaat ttaaaaatca ttttagattg
    20341 tgcgaatttg atgcataaag aaaatcatgc tgatcaggaa aaagtgatcc gaggtgcctt
    20401 ggacaattta catggttata ttacagcgct tcatctcaaa gactatatca tggaaaatgg
    20461 aaagatccgc atggtacctg ttggcaaggg ctgtttagat ttccgcccgg tactgcatta
    20521 tatcaaatat gagcgtcctc taatgtacgc cactcttgaa gctactcctg agatttatgt
    20581 tcctgaagca atagaacact tgcagtttat ttaccaagat tgttaaggag aagattatgg
    20641 aggaacgaat acagaaaagt tatcaaaaat atcatgcaaa tgcttttcag acattgctgc
    20701 atctttatca gggaaattat tggcgttttg ccatgtcagg actctttttt attatcaaac
    20761 atcttccatc ttggttaatg ccgatcgcta ttgccaatgt aatcaatgca gcttcatcga
    20821 aagattcttc tcagttgcag gccatttatt tcaacgcttt tttgatggct tgtctgattg
    20881 gtcaaaatat attgacgaat tattttcatg taaaatacca tagcacttcg atccgtcaag
    20941 tagaagctgg actaagagcg acccttatca agaaaatcca agaactatct attccttatc
    21001 aaaaagaatt gcaatctgga cgaatccagt caaagatcat ccgtgatgta gaagctatcc
    21061 aaacgttgtc ctctcaagta tttgtcagcg gattaaatat cgctgtcaat ttattcgtag
    21121 ctttaggtat cacgctttat aaaagtcctg ttgttttcat gtttttctta ttgactgttc
    21181 ctctcgccgc attcgttact ctctttttcc gtaaaaggat cagacagtcc aactcagact
    21241 tcaggcaaga aatggaagag acttctgcaa aagtgatcga agtgatcgaa ttagcaccga
    21301 ttgctcgagc tcatgggtta gaaaaacacg aaacaactcg cttaaataag cattttactc
    21361 aaatctatga aaaaggattc cacttagata tcctgcaatc tgttttcgga tcagtcagct
    21421 gggctagttt tcagatattt cagctgattt gtttgatttt cactgggata ttagctttac
    21481 aagggcgcat ccaacccggc gatgtcgtga tgtatcagac gtatttcacg acgatcgtca
    21541 attccgtttc tggaatgatc acgctgattc ctacgatttc aaaaggcatg gaatctatca
    21601 cttctattgg cgaactgttg attgctggcg atgtagaaac ttatcacggt aagaaaaaaa
    21661 tccaacattt ggatggaaat tttcgttttg aggatgtttc ttttctttat ccggactcac
    21721 tcaaaccgat tatcgatcat ttttctctag aggtgaaagc tggagaaaca attgcttttg
    21781 ttgggccatc tggctctgga aaatctacac tccttaactt attgattgga tttatccagc
    21841 cgacttcagg aaaaattttt ctagatgatc agcctttgga tgaactggat atgcgaagtg
    21901 tgcgaaatta tttagccgtt gttcctcaaa ccactttgtt gttctccgct tcgatcaaag
    21961 agaacatcac ttatggcttg aaaaacgtat caaaagaacg tttagatgaa gtcatcgaag
    22021 ctgcgcaact ttcttcactg attgaccagc ttcctgatgg tctggatacc ttagtaggcg
    22081 aacatggcaa caagctgtct ggtggccaaa agcaacgtat ctccattgca cgagcgttga
    22141 tacgtcagcc aaaaatcatt ttgctagatg aagcaacctc agctttagat aaccagtcag
    22201 agaaaaaaat ccagcaagcc ctgaattacc tgacagaaac acccactact tttattgtgg
    22261 ctcatcggct ttcaacgatc aaagaggcag ataaaatcgt tgtgatcgat gaaggaaaaa
    22321 tcgttgaaat aggtacttac gaaaaactgt tagctcaaaa aggctatttc taccgcttga
    22381 aaaatgaatg acaaaaaata ggagctatga caagactttt gtcgtagctc ctatttctga
    22441 ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatagtcat caaacatgag
    22501 aagctgtttt ttgaaattgt tccttgtaac tcagagttaa acggtaggct cacgacctct
    22561 tggtaaatgg tgttcaagag acagcctatc ggcaataaga agcaattttg tcatcgctca
    22621 aggccgaacg tctctttcac accctctgtt gtttcataaa atagttttat tcgctgtaaa
    22681 ggtcgcgaac tggtgtcatg atgatacggt tcaagtcatt gatgattgta ctgaagcctt
    22741 gctctttttg catcaagtca ttgattactt cttctgattg gattttcaca gccatttctt
    22801 gtgctttcat tgcatcttcg tcgctgaact cttgtccact catttgtttt tcttgtaatt
    22861 cgatttgcat cgcttggaag tctttgaaca gtttgtgtgc ttcttcgttt gcttgtactt
    22921 tctcgtacgc ttctttcaac gctttgaatt ctggtaattc acggatctca cgttcgattt
    22981 gattcgcgct atcgtagatg tttgacatgt tatgccctct tttcttttag tagttactct
    23041 ctattgtaac atggggttat tcgttttcaa aggggctact ctccttgata tttccgccag
    23101 aggtctttag ccgcttcacc aattccctga acgccttctt tcaccatatc gccgaagttt
    23161 ttcagccctt cttttgcttg ttcaccatat tgattcaagt tatcttgcca atttccagac
    23221 tctccattag aatttcctgt gtttccaact tgatctgcag ctaccacttg tcctcctgtg
    23281 gcataggcat ctgctacact aaatcctgtt tttgaagtat acggaaggat gccttctgcc
    23341 gctgatttga agactttccc gacaacattt ccacttgtcc cttccaaata gtgcagcttg
    23401 ctggattctt cgtaacccat ccatgtcgaa atcactacgt ctggcgtgta accgatcatc
    23461 cattggtcat ttgcttttgt ggcatcgaaa ttagtttctg tggttcctgt tttccctgct
    23521 atcgtataac ctgcaggatt cgcagcagcc gctgttccat ttgaaaatgt accaagaagc
    23581 atgctggtca tttcatcggc tgtttccttt gatatgacct gttttggttt tttgtttgtg
    23641 ttatcgacga tcaccgctcc agtcgaatca acgattttcg tgataagatg cggcgtatac
    23701 atctgaccat cattggcaaa tgcactgtaa gcacccgcca tgatcaatgg agatactcct
    23761 ttttccagtc caccaagggc taagccataa tacttatcag attttgctaa aggcaggccg
    23821 aattcttctg ctttatcata tcctttttgt aaaccgatct catgcaaaag ccatacagta
    23881 ggcaaattca aactttgtgc aacagcttga tacatcggta cttctccttg gtatgtcccg
    23941 tcaaaattat gcgcatcgta ataactttgc ggttcgtctt tcaagatact ggaaggcttg
    24001 tatccagctt ccagtgctgg tgtatatacg cttaatggtt tgatcgttga tcctggtgaa
    24061 cgacgcatct gtgtagcaaa gctgaatcct ctaaagacat gatctcctct tcgtcctact
    24121 agtgcttgta caccacctgt attcggatct aaagctactg aagcagattg gaccatggca
    24181 ccatctgaag catttgctgg gaataatgca ctgttttgat aagtagcttc taattgcttt
    24241 tgataatcct gatctaaagc tgtatagatt ttatagcctt tattcatgac atcttcttca
    24301 tcaagaccat actcgttaac tgcttcatcg atcactgcat caaaataata aggataacga
    24361 taaccagaat ctgaattatc gtatgtgtca ttaagatacg cgctgatatc tgtctggctt
    24421 tctgcttgtt cttgttcttt tgtgattttt ccgttttctt ccattacact tagtactgtg
    24481 tttttccgat cattcgcatt ttttgggtga tcgatcggat tataaatacc aggacctttc
    24541 agcattcctg ccaaagtagc agcctcagac agcgtaacat tcgttgcatc tactccaaaa
    24601 tactttctgg aagcgtcttg gacaccccat accccgttgc caaaataggc attgttaaga
    24661 tacatggtca agatttcttc tttgctgtac ttcttttcga tctcgattgc caaaaataat
    24721 tccttcgctt ttcgatcaaa cgtttggtct aatgtcagat acgcattttt ggcaagctgc
    24781 tgagtgatcg tacttcctcc gccccctgat gttccgccag acaagaccat tcggactgct
    24841 gcacgggcaa tccctttgat atcaaacccg tgatgctcgt aaaaattcct atcttctgtc
    24901 gagattactg catcttggat atagggagaa atcgcgttta attctacata tgttcctttt
    24961 tgggcgtaca gtgtgccagc ttcttcgttt ttcttatcat aaatgactgt cgattggctt
    25021 aatcctgatt ttaacgtttc gacatttgct tgttttgcta aaataaagag gtagatactt
    25081 gtcactaaga cacagatcaa accaatcagc aaaatcaatt tgttcacatg atattttttc
    25141 cagatgcgct ttcgccatcg atggaattgt ccgagatatg gtctgatcca acgccaaaaa
    25201 ctgatcaaag cagcttttat cttttctaaa atgctttgaa aatccatttg cttcttctcc
    25261 tgtctatttg attcaatcgt cttattttac catagtgaaa gagtgttttc ttcctgctta
    25321 agttgtagtt caatatgttt ttttacgtta gtgcaagtag agtaacataa gaatgaacag
    25381 aaaaatgtaa gttttttctt aaatatgttt taaaaatggt acaattagtc tgcgattgca
    25441 ctgaaaggag tacacttgtg aaaatcacga tcactttacc tatgaaccat cctgaaacaa
    25501 caattcgcga cttgcttgaa aaggaatggt tagttcctag aaaggttcga cactttttgc
    25561 gtatacgtaa aaacgtttgg atcaatcaac aacctgcttt gttccatcat gaagtaaagg
    25621 gcaatgacca gatcacattg ttattcgaag aaacagatta taattatcaa gaagttcaat
    25681 taggagatgc gaagcacatc cgtgttttat atgaagatga gcatttgatt atagtggata
    25741 agcctgcagg aatgaagact catccaaatg aaccgacaga aaacagtacc ttactcaatc
    25801 atttagctgc ttatctcgac actaaacagc aacgacctta cgttgtccac cgcttggaca
    25861 aagaaacaag cggtgccgtt ttatttgcaa aaaatccatt tgtcttgccg atacttggaa
    25921 gaatgttgga acaaaaaatg atctatcgga gatatcaagc catcgtatgg ggagaaattg
    25981 ccaaagatat gaccatcacc aataaaattg gacgagatcg ccatgatcgg agaaaaaggg
    26041 tcgtagatga taaaaatggt cagagcgcct tgacacatgt cactgtcaac aaagtacttt
    26101 ctgaacaaac acaagtctat tgtgttttgg aaaccggacg tacacaccag atcagagttc
    26161 atttagctca taagggacat ccgatcgttg gagaccctct ctaccaaaat agaacagctg
    26221 aacgactgat gctccatgca ttagaactgc acatgatcca tccatttact caagaaaaaa
    26281 tagtcggtaa agctttacct ggattatggg aatagaaata aaaaagaaca gagtcttatc
    26341 agcagataat gataagactc tgttcttttt gtgtaatttg tctgcttact tttgatgttc
    26401 gtgttctgct aacagcaaac taccgataat cccactttca ttcggcagtc cccaacgaac
    26461 gatatattca cttacttgag gtgtttccat ataaccaccc atcaattcaa caaattgctg
    26521 acggattttt tgaaggagat gatcctgatt cattacccca ccgcctaaaa taatcttttc
    26581 aggtgctaac gtcaacgtat agttgatcag tgcttgagca atataataag cctgaatagc
    26641 ccaaacagga tgatcttgcg gcagatcttg ccctttgatc cctgtgcgtg cctcaataga
    26701 cggtcctgct gctaatcctt ctaaacaatc tttatgataa ggacaagtac cttcataatg
    26761 gtcatctgga tggcgtttca cccaaatatg tcccatttca ggatgtgcga tccctgaaaa
    26821 aatttcttca ttgatcacga cacctgcacc gattcctgtt cccacggtaa ggtaaataca
    26881 actatttttt ccttgtgctg ctccttgctt caattcccca tatgcagcag cattgacatc
    26941 cgttgtccaa gcagcaggaa tattgtagcg ttccttgatt gtgccaagaa aatcgaattg
    27001 tgcccaccct tttttgggag ttgccaaaac gtatccgtaa ttatcttttg ttttgtcgat
    27061 accaatcggg ccgaatgagc caatcccgat tcccttcaat tcataacgat caaaaaattc
    27121 aaatacctga tccaatgttt cttctggtgt tgtggtaggg atacttaatt tttctaaaat
    27181 ttctccacca tctgaaaccg cacacacaaa ttttgttccg cctgcttcta ttccgccata
    27241 cattcgtttt cctcctcgaa tccgattgtc cgaatctcat aaggttggat tattgggaca
    27301 accgcttgct tcgttggttc ttcaagcaga ttcaatatga cagtttttcc attgtcttta
    27361 gttatttcca atttttctaa atgactagac atattgaaac ctcttactac tactttatca
    27421 ctaaacttgc tgcgtttcaa agctgtaatt gcaaacgttt cactttttat cgttaaatat
    27481 tgttgttttg aaatcaatgt tccttcatga tgtttgattt gttgagtgga aaaaggaatc
    27541 tgtgctgcgt atgcatgcaa ataagtagag aatttttctt ctggtccatg tagttcaatg
    27601 ctgtaattga accgatgctc acctagacac tgtgcttctg gtgttggaaa atatccccaa
    27661 tcgcctagtt ctcccacact gcgaagtagc gtcactgcga tttgtccgtc ttctgttact
    27721 tcatattcat tcaaaccaaa gtttccgact gttacaccgt actcttcatc atgaatattg
    27781 acaaatgctt gctgatgttg cggattcgtg gggttttccc aacttttaga cacatggttt
    27841 gggcgcttca ccacttcaaa aatactatct gcttcgtgat gttccacatg taactttgta
    27901 ggaaacaaga cacgtaagcg atgatctttc atctgattgt cgagagtcgt ttcaaagtcg
    27961 atttttttac tatcttttct gattgtcatg attgttttta tttgaaggat tcgtttttct
    28021 ttggatcgtt ctgcttttcg ataacgaaat tccacaacca tctgctgttc tttttctaaa
    28081 cgctcgtctg cagaaacagg gatttccatt tcctgtagaa tacttatttt tgctatttcc
    28141 ggtgtatcta ctaatacttt tacttcagag ttttctttgt tactggatag gattggctga
    28201 tcacagaaag gttgtttaaa gatatactca tttcctatat cgccgatatc ttcaaaaata
    28261 tgcagattat tcaactttcg tttgtttttg cgatcttcca ttgtaatggt accatttttt
    28321 tcaatcgtca aatgtagagg gccgttttcg atgattcgtc cactctgatg aatcattgtt
    28381 tccttttctt ctgctttcgc ttccccttct acaagcgcga atgtttccca agaaaatgct
    28441 ggcatgtttt ctaatggtaa agtcactttg atcattcttg ccatatatgg cacacggaaa
    28501 cggtctttcg gcaaatcata accaaattgg acagtttctt cagataaaat agctggtata
    28561 gtggccccac tttttgtttc tacatgatac ttacgtttag gcaaattttc taactcttga
    28621 tatagttgtt caggtattcc ttcagcgaat ctttttcttt ctaattcgat ggttatttct
    28681 gcttcaccag ttttatccat tcctgcagta tttacaatga caaatggaaa gctgtctttt
    28741 gggaatccgg tcgtatcgat tgctgcggtc agctgttcta aggaatcttg tgcaagatac
    28801 tttccaactt catctgcttt ttcaaaacga ggtatcattt cccgatggac agaatcgact
    28861 gaacaaccgc aaatactgtc gtgaggatga ttttgcatta atgtcttcca agcataatct
    28921 aattgatcat ggggatagtt tccactaact tcataagcca tcgtcgcaag tggttctgta
    28981 atattttcta gctgtcgctg gaccttggta ttccattgtt ttaagtaaac ccgagcagat
    29041 gctgtattgg ctaaagtata ccagccatcc gtttcttgac ttgtcaattc tccttctact
    29101 gagcctaagt cttctggcac atctttttga actgcttcca gatagtcagt aaaattagaa
    29161 tgaataaact cgtaatctgg aaacagttca tttgcgagat ggattgcttt tgatatatct
    29221 ttttgtaccg gttgatgatc aacaccattc atcatcaata agtggttcgt agaggcatat
    29281 tgttctgcat ccgctaattt ttgcttccaa aaggcaagag ctgcttcttt ttctactgga
    29341 atctcattcc cgttgctata ccaattggca aatagcagac caaagattgc tgtttgatca
    29401 ggtcctttcc accacatctc tgagtattgg gaagaatagt tttctgcttc caaaacttga
    29461 ttatcaaagc caattggttt tacaccacgg ccaaacgctg ctgctgaaat accagcttgt
    29521 ttcattaatt gaggcgtctg tcccatattt ccaaatgtat cgggaaaata gcctaacatg
    29581 acaggggttc cccatttacg gctttcttcc atgccaatca gcatatttct cacgttcgat
    29641 tccgagctga taagaaaatc atcttgcaag atataaaatg gtccaattct caattttcct
    29701 tcattgattg cttgttggat tgcctgtctt ttttctggcc ttacttgaag atagtcatct
    29761 aaaataatcg tttgcccatc caaatgaaaa ctgttgaagg acggatcaac ttcaaatagc
    29821 tctagtagat cgtctatcaa ttcaatcagt cgcatgtgat gctgttcgta agccatgtac
    29881 cattcacgat cccagtggct atgagaaatg atatatactt tctttttcat ttacgatacc
    29941 tcctactttt ctactctaat atcaaagtaa tccatgacta attcgcaaaa catcatattt
    30001 gcccaagaaa accattctct cgtataattt tcaggattgt tcacatcgaa tccttcgtgc
    30061 ataaggtgtg ttcctgcgtc agttgctaca agtaaattca atatccgctc tttttcagat
    30121 ttgtctgacg ttgtcattcc ttccatagct aatgcaatcg gccaaacgta tttttctggc
    30181 gtatgggagg atccaattcc ttttgcgtac tttccttcat agaaatatgg attttcttta
    30241 ctcaacaatg ttcttcttgt ttgcagataa tcttcctctt gttcagaaca atatcccaag
    30301 taaggtgcgg cgatcagatt gggcacatta ctgtcatcca tgactgtggc gtttcccatt
    30361 ccatctactt catatgcata aatcgtttgc cctttttgat taaccgttcg tccgtgattc
    30421 tgaatcccct cttggatctc tttcttcaat ttctgtgttt gctgtacaat ttctttatca
    30481 tcgagtatcg tttgaaaaat ttcttccaca taacctaaca cgaccaccgc aaacatattg
    30541 gatggtacca gatatccata acggcaagta tcatcgctag gacggaaacc agaccatgtc
    30601 attcctgtaa aagctgtttt tgcacctcgc ccgtcattga tcagcgtatc ttcttttctt
    30661 gtcgtgtctc gtacaaatgt ataaggagag tgttcatgat cttgctctgt tttgaacacg
    30721 tccaagattt ttttgatacc ttcaataaaa gattgattga attgatcagt ttttcctgta
    30781 tttttgtaaa gaagataggc caactgtatt gggtagcata aagaatcaat ttcatatttt
    30841 ctttcccaga tccaatcgtt catttttgta tgatctgttt ggtggccagc accgttagct
    30901 tcttcgttga aggcattcgc atatggatcg atattgatat aaaaaaattg ctttttgact
    30961 aacccgctga tcatgtcagc taattcctca tcttcttttg caatcactaa ataaggacga
    31021 acttgtgcag tagagtctct taaccacatc gctggaatgt ctcccgtcaa taaaaaggtg
    31081 gttccgtcat tttgcttttt gactgttgtc aacagagtat tggcaaatgc tgctgtaaaa
    31141 ttctttgccc agtccgcatg gatatcccca catttttgtg tgatatcttc cataaatttc
    31201 tgcaccgatt ttggtatatc tttataagcc actaataaaa ccttctttct atttgatata
    31261 tctttaaaga ttatacttca tacagccaat tatgtctacg ctttcaaaaa atatcttttt
    31321 atttactttt tcatccatta tggtattatt tgaatatgat atatcattaa gaggtgttta
    31381 tatgaatcaa ccattgtacc agacaatatt taatgatttg caaacggcta ttttaactgg
    31441 tgaattacct gtagacagcc aactaccaac tgaaaaagaa ctttcacgta cctataaagt
    31501 aagcagaatc acttccaaac gtgcattgac tgaattagag caattaggac ttatttatcg
    31561 cgttcgcggg aaaggcagtt tcgttaaatc cgcttctccg gaacagattc ttgcttctcc
    31621 tacaaaaaca cgacggattc tgtttcttct tccatatgtc gctgatttgt ctgttggtga
    31681 ttttagcaag ggattgaacc ctgtgatgca agataaacag attgatgtca tgatgacgac
    31741 gcttgacttt ctacaaaata aaacagcaaa agagctaatc aatgaatttg acggattgat
    31801 ttattatgct cttgatacag aacaacatct tgacttactt tttgagcttt cattgaaaga
    31861 attccctgtc gtcatattag ataaaaaaat ctatgagtta ccttttccta ccgttctttc
    31921 cgataatttc caaggcggtg cgcttgctgc ctcttatttg atcgaacagg gacattcaaa
    31981 aatcgcttat ttgtttggta cgcagactca tccacaatcc gttcgacagc gttaccttgg
    32041 ttaccttgaa gcattggaca aagctggttt gactttccac acttcactgg atgacaaaag
    32101 cgcgacatta gatgaattgc tttcttatat cactaagtat gaagtaactg cttttgtttg
    32161 tgaaaacgat gttgtcgcta tccagaccat gaatctcttg agacagcatg gttatcatat
    32221 ccctgaagat ttttcaatta tcggatttga tgatatccaa gcagcagctc ttgttgaccc
    32281 gccactgaca accatcgcac aagattttga acagttaggt aagcttgcgg gagatgcttt
    32341 ggtcacttgg ttagagacaa aagatcgacc atccgatagt aaatttccag tttcattcat
    32401 caaaagacaa tcaacaaagg agaaataaaa tgaatatcgg tcaaatagat actagacatg
    32461 caacagcgaa tcagccaaat ttctctaatg ggaactgcca gccttatact ggcgttcctt
    32521 ttgggatgaa ttattttgca ccgcaaacta cggatcaaaa cggcagctgg tggtttcatc
    32581 ctgatgaccg cgtttttcaa ggctaccgtc ttacccatca accaagtcct tggatgggtg
    32641 atttcagtca tatgctgttt acgccgatca atggaaaact acaggagaac acccttttcc
    32701 atgctcaaag ttcttaccgc ccagaagaaa gtatattttg cccgactcat ctgtcgatct
    32761 gtcaattacg ttatggcatc tgttctacat tgatacctag catgtatggc ggaatcttat
    32821 ctattgatta cagcaaaaat gacagcggtt tacttttatc ctttccggga cgccatcagc
    32881 tctttgttat agatccttac acagtggaag gaaaaatcac acattttgct ggttcagaag
    32941 atcctgattt ttctttttat tttatattga gatttgaata tcctttaaca attgatgagt
    33001 tagctgtttc caacaaaaat agcgactcga tcccgttgta ttttgaacag acaaaaaaac
    33061 aaactatccg atttgggaca tcttttatca gctcggaaca agcagcttat aacctggaac
    33121 tagaacaaaa ccaaagcaaa gagacttacc ttgctcacag tcaagaacaa tggcagtcct
    33181 attttgatcg tatacagctc acacaccatg atcaacaaca attgcggacg ttttatcata
    33241 atttatatcg ggtgttttta tttccacaaa cattttatga aattgatcct agtgggcaaa
    33301 aaatccacta tgatacgact agtcgatcta taaaacctgg tgttctctat acaaataatg
    33361 ggttttggga tacgtataaa acggtttatc ctctttattc tttgattgct gtagaaaaat
    33421 acgaagaaat gctggaaggt tttttgaaca gctatagaga aagtggctat ttgccaaaat
    33481 ggctgtctcc tgatgaacgt ggacttatgc ctgggacatt gatcgatgcg gtcattgcag
    33541 atgctgctgt aaaagatatc cgacgagact tgatgcctga atttctaacg gcaatggaaa
    33601 aaggtgctac ggttcaaagc aaacatccaa attatggacg ccaaggaaca gaagattact
    33661 tgacatatgg ctatgttcct aatcactacc acgaatcagt caatcacaca ttagactatg
    33721 cttatagcga tttttgtatt agccaagttg ccaaggtact agaagaagag gaaatgacga
    33781 agaaatatca gcagcaatca ttgaactatc aaaatatctt tgactatgag tcaggattta
    33841 tgcgttcaaa agataaagat ggcaagttca gagaaccatt ttcagctaat cgttggggac
    33901 aagattatgc agaaggaagt gcttggcaaa gcagctttgc tgtttatcac gatttttcag
    33961 gactgatcgg aaaatatggt ggcagtgaga gatataaaga aaaactcatt gagctgtgca
    34021 atcagccccc aacgtttgac ccaggaggat atggttttga gatccacgaa atgagtgaga
    34081 tggccgcgat agaatttgga caattagcta tttcaaacca gccaagtttt cactatcctt
    34141 acttgttcag ttatatagga caacctgaaa tggcacagcc actgcttaaa cagttgatga
    34201 ctcagacctt cgacgattct ccaatgggtt accctggaga tgaggacaat ggaagtatgg
    34261 ccgcttggta tatcttcaat agtctgggat tttatcctgt cacgcctgga accggtgaat
    34321 atgttatcgg tatgccatta attgacaagg ctgttttgct tttgtcaaat ggaaaggaat
    34381 tgacgattga agcaacgcct aatcggccgc agcaacaatt tatccataaa atcaaacgga
    34441 tggacaaaga atatacgaaa ttatatttta cccatcaaga tttgctgaaa ggcggcacga
    34501 tctcttacca attagggatc atccctcacc ctaagaaata tcaagcaaaa gatttgcctt
    34561 tttccttatc aaactataat taacaagagg ttgtgaaaaa gctgtcctgc atcaagtaat
    34621 aagaacagcc aagcaaaaat agatcctcat atttttcgtt tggttggtct tattaccgca
    34681 gatgcaagct tttgaacacc gtttattgag aggttgtgaa atcagcgttc tgacctgagt
    34741 attagagaga caaatggcaa aatagttcct catattttgc cattttttga gctaataccg
    34801 agggtcagct gattgaacac cgtttagtgg agaggtcgtg aaaaagctgt ttagtttcga
    34861 gtattaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tggcttaata
    34921 ccgagaaact ggcttttgaa ctccgtttat cgagaggtca tgaaaaagct gccttgcctc
    34981 aagtaataag aacagcccaa aattagattt ctagacaatt ttttataagt tatttcatta
    35041 cattggcttt ttcttttatg attctccact gttttctcgg tactctttag gggatctgcc
    35101 gacgattttc ttgaataatt tagagaaata cgtggtgttg ttgtagccaa tctcacttgc
    35161 tatctcattg atgttttgat tactgtgcaa aagcagttgt ttcgcttttt ccatacgtaa
    35221 atggttcaaa taagctgaaa aacttttttt cgtttctttt ttgaacagct gtcctagata
    35281 catgacattt aaatgcaggt tttctgacac ttctttgagt gtaagtggtt cttgataatg
    35341 ctggtgcagg attacgatta ctttttccac attgtcactg tactttttct gtctctgatg
    35401 ctttttgatg aatgacagaa gtagctgctg aagttcttgc accgtttttg cctgatggat
    35461 tttttcgatt ccttgtaaat attcctcatc ttccagatca atcaattccc gttgaatctc
    35521 cataaacagt aaaaaactgg aatatcgtat atcttccggc atcatggctg ctaactgaaa
    35581 ctgttcaaaa aaatcagtga tcattttttg agcgacttcc aattgtttat tttgcagcac
    35641 gcgtctgaaa cttgaaaaat cgatggtttg atcttttgtt tctgcttgta ctgcgtaaaa
    35701 aacatgctcg tctttatctc cataaaattg atgcaactgt agattgtctt tcgcttgttg
    35761 gaatgtttca gggattttgt ccaaagaatc tgtctcttcc ccgatactga tcagccattc
    35821 ctgatcagga aagttttctt gaacaaaaca acagaagcca tcaacttcat ctgcctccaa
    35881 caacaacgtg atcaatacac ggtctccgta atttcgttga tagtaaaaga tttgttgccg
    35941 ttcttgcaac catctattga taagagtatc atgagttctt gccagttgaa tcaatactat
    36001 cctatgctgc ttctcactta tttgtcctat tatttcttct tcacttattt catccaattc
    36061 ttcattcaac cattgactga ataacagttc ttgatagatt tcttgttgat ttttttgttc
    36121 attttgctga tctaaccgtg ttttgatttt cttgaggctt tcaaccaatt cgaacttatt
    36181 cacgggtttc atcaaataat tcacggcgcc taattgcata cctcctttca aataatcgaa
    36241 ttcttggtat cctgataaaa tcataaattc aaaattatga cgttcttttt gagccgcttc
    36301 aataaattcc agtccattta tttctggcat tgtcacatcc gttactacta aatcaatctg
    36361 tcgctgttca agtacagcta gtccttcttt ggcattctct gctgttgcta ctatttgaaa
    36421 gcctaaactt tgccaatcaa ttatcttttt taacccattc aaaatcatgt attcatcatc
    36481 taccaataac acttgataca cgctcatccc tccttgaatg taatagaaac ggtcagtcca
    36541 cctgtctcat tattctcaag cgacatataa taactgctgc cgaagctggc acgtaaacgt
    36601 tcattcacgt tttgcagacc gatagaaccg tgaagttcca ctttaggatg agacagtttt
    36661 tcttcaatct gttttagccg tttttcagtc atcccttttc cattatcttt gatgatgatc
    36721 cttacccgtt tgccttcctg caagactttg acacttagcg cattatcgaa tcgagtaaaa
    36781 tcaataccgt gtttaaaata attttcaact aatggctgga tcacaaattt aggtacttcg
    36841 atcttttcca aatcaggatc gatcataaaa tgataggcta ctcgattagg ataacgcatt
    36901 tgatacaaat agacatattt ttcgcaaaag tccagttctt cttttaaagt gatcgttttc
    36961 tcttgattcg tattgtttcg aagcaaagcg gaaaacgcat acacaacatc tgctaattct
    37021 tcacttcctt cgctgattgc atacattcgg atatattcta atgtattata taaaaagtgt
    37081 ggattgatct gtgcttgaag cgcccgcata tgtgcgtctt gctgtttgat ttctaatttg
    37141 tagatgtctt ctacaaactg attgatgcta tccaacattt cgttgatacc gattgaaagt
    37201 tccttcagtt catattccgt gtttgttatg tcgatccgcg tctctaaatt ccctgctgct
    37261 gtttgatcca tggcctgcat gacgatagat acttgctgtg aatatcgttt gaatgtttta
    37321 taaagaaaga taagcagtat attgtctaaa ataatgatac caatgatcag cggggcaaga
    37381 ttttttcccg tttcgatttt tacacttttt ttatctagta cagctaatat acgataatca
    37441 tcagacagtt cttggtgttg aacaaaattg tttttttcca gatcgtcaat cggcaattgt
    37501 gaatttttct tcagactctc tgtgatgatc atttcttgtc tttcaaacgt ttctttagct
    37561 attttattat catggaatgt gtatagtcga tttgtcgttc cagaaatcat gtataaagag
    37621 agtccattga atgacgttag attttctaaa atcatatcta gttctttctt ttgaaaacca
    37681 ataaaaatac ttcctgactc agctcctcct tgtgtgatag gcaacttgat ataaaaggcg
    37741 tctagtaatt ttggtgtccc cttttttatt tccccacctt tgttttctct agaagattca
    37801 taatactccg gtagttgatt caacacgata tatatcgcac ttagttcttc ataatttgcg
    37861 tagagatctt tgatttgatt tgggaaagaa actaattcat cggtactgtt ttgctgttcg
    37921 tatacataca tcagatactg atctatgggt tttgttaagt aggtcgtcac attttcgatc
    37981 ttttcggaat tccctacaag ctggtttttg atgatcctac tcaaggttgt tttatcattc
    38041 agcattctgc ttgtgctttg aacggcatca cttgtcatgt tttctgcctg tttttgattt
    38101 tgttcccaag tatggatact aaaaataacg gtagcagtca tcgttacacc gatcatgata
    38161 accgcgtatt tccgcaacag acgatttagt aaggttcctt ttgaaaatag aatccgaaac
    38221 ttattcataa ctttccaact gtttatctaa ttcttcacgc caatgtgttg tcatcgtacc
    38281 attccaaacc gtcaaaagac caatgacacc aaacaggatc aatcctttga attgccaagt
    38341 cacgcctaaa attataccgc taccaataat tatttttaga aaagacgaaa agctggaaaa
    38401 aaccgaaata aaacttagct ttagcagatt aggaacagag atcgtatatt cactatctaa
    38461 aatcatactg tattgataag tagcaaatag gcagagcaag gcaaaaagta aaataaaatc
    38521 aatagctaaa aaaatgagcc ctttgatttg aacagataaa tataaattgt aactcaataa
    38581 agcagtacca gataaaaata aactaaatag cagattgcct cttaaaaaat ttcttttata
    38641 tatattccag cctcttttta ttgtgatttc tttatactcg aatccgtaca aataaaatag
    38701 ttcattgact gttttccatg ccgcacctaa tccaaagatg atcccgccgg caaatgtata
    38761 tatccagaaa tacaaggtta gttttataat gacccagatc cggataaata actcttccaa
    38821 aaccttccct aacatatctt ctcctccaaa cattaagttt atactttctc attatacaaa
    38881 aaaaggttgg gcaatcagca ccttgacctg attattagag aaacaaacgg aaaaatagtt
    38941 cctcatattt tgtcgttttg tgagcgaata ccgagggtta gctgattgaa caccgtttac
    39001 caagagtttg tgaaatagcc gcttagtttc gagtagtaag aaaaattttg tgaatatggc
    39061 tttccacatt tttacatata tttagcttaa taccgagaaa ctggcttttg aacaccgttt
    39121 attcggaaca agaggttgtg acacgtcttt tgtcacaacc cctttgtctt tattcattat
    39181 tattcttgtc ggaactcatc atattgtttt tgcatttctt tcaatacctt atcatagcca
    39241 gccttgtcca aagcgtcttt caatttcggt aatgtttcat ctggatcaac agtccctgta
    39301 ttcaatccat ctaaatattg attcatgaca ttagaaatat tacttaattc tgttttgaca
    39361 ctgtcagtgt tgaagctgaa tcctaaaatc ggtgaagttt ctgcatcagc aatcgattga
    39421 tcacgtttgg caatcatgtc atccgtaata gactcttgtg tatatagaat cttgttgttt
    39481 cctgtattcc aagcagacat atgtgtattt ggctgataac catctagcaa tttgatttta
    39541 tcatctgaac catcgacttt ttcccatgct tctccctcga tcccccaaac aagcccattc
    39601 aataattctg gatcactgtt aagcagccct agaagttcta ctgatttttc tttatttttt
    39661 gaagtatttg atacgacaaa atttgccatt tgtgcttgag ctgtagattt tagtggaaca
    39721 gtaattgctt ttgagacaag ttcttgtttt gctgcatttg tcaaaattgt atcgccataa
    39781 tcatacggtc cttgtgtttc tccacgcatt agccaagtat ttccttctag tggataatcc
    39841 ttattgctcg ttgcagcgtc acttggaatg agtccttgtt tgtaccagct atgcattgta
    39901 cgtaacagtc cgatatacgt ttcattatcg tattggttga tgattttcga atcatctcca
    39961 tccagatcaa tagcaaatgg taacgtattc cctaaaggat aatcaaagtt cccttggact
    40021 ttgaagcctt gtccaatggc aaaagctgcc gtgtttggtt ctttttcatg gaattccttc
    40081 aagacttttt ctgcatcagc gtaagattgg attccatcaa ttgaaagatc gtatttatct
    40141 agtaaagctt tgttaaaagt aagtacttgt tgagcaaaga catttccatt gactgggaaa
    40201 gcatataatt ttccattgac cagatttcct ttaatatatg cttcatctaa atcattataa
    40261 gctttttcag cgtacttagg cgctaattca gtcaaatcag caaaggctcc tttttgggca
    40321 ttggaaacat agttgttcgc aaaggcgata tcataatttt ccccggaaga aatgatgacg
    40381 ttcattttct tttcgtaatc gccccagcca atgtaattga tattgacttt tacaccgatc
    40441 tctttttcga ttcgtttgtt tgctacttcc atcaattgat cgaaattatc tggtttatct
    40501 ccgatttgat acatctgcaa agtgtccgaa ttgctagctt ctgcctggtc tttagaacca
    40561 gaacctccgc atgctgctaa tgttcccaac aacaacgctg aaacacctgt aaaggctagt
    40621 ttcttccata acttcatttt tcttcctcct aattttatga tttatatttt tgtttcttta
    40681 ttcttttact ccgccgattg ttagaccttt gacaaaatgc ttctggaaaa atggatagct
    40741 gatcgcaata ggcaacgtcg agatgactac gatcgccatt ctggcagatt ctcctggaac
    40801 tgaggctaat ccgcctgtca attggcttcc tgctcctgaa ttttgtgtca gatattgaat
    40861 attactttgg attttcatca aaagatactg tagtggaact agcttatcat cttggatgta
    40921 tagtaaagca ttgaaccagt cattccagta acccaatgcg gcaaataaac tgattgtcgc
    40981 aatacctgga acagctaacg gtaaaacgat ctgaacaaat attcgcagtt cgctagctcc
    41041 atcgatccgt gccgattcaa taatcgaatc agagaccgaa cgtttaaaga atgtgcgcat
    41101 cacaataata ttgaagggac tgacagccat tggcaagatc aatgcccaaa tcgtatcttt
    41161 taactgaaga agatttgtca taacaagata attggccacc atccctggtg agaacaacat
    41221 cgtaatcaaa caaaaaacgg taaaaaaacg acggaatgga aagtttgacc gtgaaatcgc
    41281 ataagcatat aaagaagtag ccgtgctatt gatcagtgta cctaaaacag taacaagtac
    41341 agtgacgaaa agcgcttgga aaattttatc ctgcatctgg tcgaacaagt acgtataacc
    41401 aaatatactg aacttttctg gccaaaaact gtacccgtga gtcgacaata tggactcatc
    41461 ggtaaaagag atggcgataa caaatataaa tggcaaaata caggaaatag caaagagtgc
    41521 aatcattata ttagagaaaa aattagccgt cttattgaaa gagcggactt ggactctctc
    41581 cactttcttt tttttcattt ttttcctcct tagaataatg cagaatcgct atcaaaacgt
    41641 cgggcgatcg tatttgtgat catcaacaag acaaagccga caactgattg gtaaagtcct
    41701 gcagcagccg tcataccaaa atctcctgtg gatgtcaggc cattgtagat atacgtatcc
    41761 aaaacttgag tcacatcata taatgcaccg gaattcctag gaatgttata aaacaggcca
    41821 aaatcagctc ggaaaatgtt tcctacggct aaaatcgtta aaatcgtcat tagtggaata
    41881 agttgtggta tcgtcacatg tttgatctgc tgccatttgc tagctccatc aaccatagca
    41941 gcttcataat aagtcggatc gattcccata acagatgcaa agtaaataat gctgttataa
    42001 ccaatccctt tccatgtacc aatgaacacc aagataaacg gccaatattt cggttcatta
    42061 taccaattga cagcttctcc tccatgtcca gtgatccact gattgaatat tcctttgtca
    42121 ggacttaaaa aagcgtaaac aaagtaactg ataatgaccc aagatagaaa ataaggtagt
    42181 aaggacatcg tt
//
LOCUS       DAGCYL010000006        39117 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.006, whole genome shotgun sequence.
ACCESSION   DAGCYL010000006 DAGCYL010000000
VERSION     DAGCYL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.006"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            35..3313
                     /locus_tag="MB216_000224"
     CDS             35..3313
                     /locus_tag="MB216_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBT4525088.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3854..5761)
                     /locus_tag="MB216_000225"
     CDS             complement(3854..5761)
                     /locus_tag="MB216_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBT4525089.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5891..6766
                     /locus_tag="MB216_000226"
     CDS             5891..6766
                     /locus_tag="MB216_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBT4525090.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6846..7820
                     /locus_tag="MB216_000227"
     CDS             6846..7820
                     /locus_tag="MB216_000227"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBT4525091.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8000..9145
                     /locus_tag="MB216_000228"
     CDS             8000..9145
                     /locus_tag="MB216_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBT4525092.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9282..9626
                     /locus_tag="MB216_000229"
     CDS             9282..9626
                     /locus_tag="MB216_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBT4525093.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9897..11183
                     /gene="efmA"
                     /locus_tag="MB216_000230"
     CDS             9897..11183
                     /gene="efmA"
                     /locus_tag="MB216_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBT4525094.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11321..11920)
                     /locus_tag="MB216_000231"
     CDS             complement(11321..11920)
                     /locus_tag="MB216_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBT4525095.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12324..13445
                     /gene="mnmA"
                     /locus_tag="MB216_000232"
     CDS             12324..13445
                     /gene="mnmA"
                     /locus_tag="MB216_000232"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBT4525096.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13691..15112
                     /gene="arcD"
                     /locus_tag="MB216_000233"
     CDS             13691..15112
                     /gene="arcD"
                     /locus_tag="MB216_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBT4525097.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15126..16244
                     /locus_tag="MB216_000234"
     CDS             15126..16244
                     /locus_tag="MB216_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBT4525098.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16304..17596)
                     /locus_tag="MB216_000235"
                     /pseudo
     CDS             complement(16304..17596)
                     /locus_tag="MB216_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
     gene            17728..18306
                     /locus_tag="MB216_000236"
     CDS             17728..18306
                     /locus_tag="MB216_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4525099.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18435..18812
                     /locus_tag="MB216_000237"
     CDS             18435..18812
                     /locus_tag="MB216_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBT4525100.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18745..20817
                     /locus_tag="MB216_000238"
     CDS             18745..20817
                     /locus_tag="MB216_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBT4525101.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21028..21975)
                     /locus_tag="MB216_000239"
     CDS             complement(21028..21975)
                     /locus_tag="MB216_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4525102.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22221..22685
                     /locus_tag="MB216_000240"
     CDS             22221..22685
                     /locus_tag="MB216_000240"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBT4525103.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22747..23790)
                     /locus_tag="MB216_000241"
     CDS             complement(22747..23790)
                     /locus_tag="MB216_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBT4525104.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24167..25132)
                     /locus_tag="MB216_000242"
     CDS             complement(24167..25132)
                     /locus_tag="MB216_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBT4525105.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25366..26211
                     /locus_tag="MB216_000243"
     CDS             25366..26211
                     /locus_tag="MB216_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4525106.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26402..27589
                     /locus_tag="MB216_000244"
     CDS             26402..27589
                     /locus_tag="MB216_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBT4525107.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27595..27951
                     /locus_tag="MB216_000245"
     CDS             27595..27951
                     /locus_tag="MB216_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBT4525108.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            27953..28291
                     /locus_tag="MB216_000246"
     CDS             27953..28291
                     /locus_tag="MB216_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBT4525109.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28367..28623
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28686..29720
                     /locus_tag="MB216_000247"
     CDS             28686..29720
                     /locus_tag="MB216_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4525110.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29713..30399
                     /locus_tag="MB216_000248"
     CDS             29713..30399
                     /locus_tag="MB216_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4525111.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30423..31271
                     /locus_tag="MB216_000249"
     CDS             30423..31271
                     /locus_tag="MB216_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBT4525112.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31468..32241
                     /gene="sufC"
                     /locus_tag="MB216_000250"
     CDS             31468..32241
                     /gene="sufC"
                     /locus_tag="MB216_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBT4525113.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32254..33540
                     /gene="sufD"
                     /locus_tag="MB216_000251"
     CDS             32254..33540
                     /gene="sufD"
                     /locus_tag="MB216_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBT4525114.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33537..34772
                     /locus_tag="MB216_000252"
     CDS             33537..34772
                     /locus_tag="MB216_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBT4525115.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34759..35229
                     /locus_tag="MB216_000253"
     CDS             34759..35229
                     /locus_tag="MB216_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBT4525116.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35234..36625
                     /gene="sufB"
                     /locus_tag="MB216_000254"
     CDS             35234..36625
                     /gene="sufB"
                     /locus_tag="MB216_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBT4525117.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36720..37862)
                     /locus_tag="MB216_000255"
     CDS             complement(36720..37862)
                     /locus_tag="MB216_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4525118.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(37901..38635)
                     /locus_tag="MB216_000256"
     CDS             complement(37901..38635)
                     /locus_tag="MB216_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4525119.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            38867..>39117
                     /locus_tag="MB216_000257"
     CDS             38867..>39117
                     /locus_tag="MB216_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4525120.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDID"
BASE COUNT        12687 a         7190 c         8256 g        10984 t
ORIGIN      
        1 aaacgctttc taatttgtta cgggaggaat acacttgtta aaaaaacgta taaaatacag
       61 tgcgctaatg atgaatctcc ttatgctcag taccccggtt ttaatttctg tcgttgaagt
      121 tgctgctaac gaacaacagt ctgtgaacga agaaaatgca cacgtgaaca ttgaagggat
      181 gtcaacaatg gaggaaatac caagaactag tgaagatatg gatacggaag aaggaaatga
      241 tcctatgatg aaagaatcag aaaatgaaaa gacaaaaaag caatctgaag aagcaaatga
      301 agagtggaaa ggggttgttt tcggagaaag cacaagtgct agtaggtatg ccattgaacc
      361 attagaagac ggcgtgcggc tctcttccac gaataacggc ggaaaattcc agtcaagcgg
      421 atcagatgga atgacctatt attatcaagc aatccccaaa gacatcaact ttaggatgcg
      481 ggcgacgatc gaaatagaat cttggaccta tacgaatgcc caagaaggat ttgctttaat
      541 ggtaagagac gcagtaccaa aaaatcattt gtttgggcag gcattttatt caaattcatt
      601 tgccatacta ggcagtcgga tcgaatatgt ctgggactcg gatagacaag aagtggtcaa
      661 cacgagcggc agcaaatata cgatgcgact tggattgggg acacgggcaa tcacaggaat
      721 atcatctgaa gatccgcaag ctgcgccagc tcctgggagt gtctcagtgg aaacaacacc
      781 tttagagact tcagcagggt tcttggaaaa agaaacaggt tcttataata tattcggaaa
      841 cggtcatggg aatctgtttc cggccacgaa ctcgattact aaactggacg tagagatgaa
      901 aaaaacgaat actggtttag aggcatatta ttacgatcct gaaacaggag aagaaatggc
      961 ttctgacatc atgtatgact gggaaaaatt atacgcttca gatgaatcgg ttatttatgc
     1021 aggatttgct gcagcaagga atatgacgat caaggtagaa ggaatcgaag ttgcttactc
     1081 agatcctgcg acagatcctc ctgggcaaga aagacctact cgcttgattg atccgattta
     1141 cactgtgacc tctagtaatc attcaggaaa ccaagatcat actatgtcct ttagagcaaa
     1201 tgctgacgga ttattgacaa ttaaaaataa agcaacagga gaggtgctta agaacgcaaa
     1261 aaacttggct atcactgcga atcgggaatt tgattatcag acaaaactga tggaaggaac
     1321 gaacgaattt actttcagtt ttacgccaga taaaaactat ccgccagaag aatacgtaga
     1381 gatgactagt tatgaaacaa aagagattct tcatacagtg gactaccgta aacctaaata
     1441 ttcgacagtt tatgtaacac cagaagggtc agatgcagga aacggaagca gacaaaatcc
     1501 attatctctg acacaagcat tgagatacgc atatgctggg cagacaatcg tcatggcgcc
     1561 tggaacttat tccttcgaaa gaggattgac gattccaaaa ggcgttaacg ggacaagaga
     1621 aaacccgatt cagttgattg cagagaaaaa tccagaaaac aagcgtcctg tacttgattt
     1681 taaaggaact ggaaatggga tgacactgtt cagtcactac tggggtttac gagggtttga
     1741 cattacaaat agtgcagcta tgcaaaaagg attacagatt tctggaaatc ataatttgat
     1801 cgaagagatt catgcttatc ggaatggaaa tacaggaatc caaatcagcg gtagctcaaa
     1861 tgatcctttc gaagcatggc ccgcgcataa cttagtcaag aactgtacat cttttgaaaa
     1921 tgccgatcca ggatttgaag atgccgatgg atttgcggcc aaactgacaa taggagaagg
     1981 aaatgtattt gacggttgta tcgcgtatca taatgcagat gacggttggg acctttttgc
     2041 taaaaatgaa acaggttcga tcggaaaagt aatcatcaaa aactcagttg cttatcgaaa
     2101 cggctgggta ccaggaattg agggagaagg taacggtaat ggattcaaaa tgggcggttc
     2161 ttctttaacg ggccctcatg aattgatcaa tagtttgtct ttcgaaaatc ttgcgaaagg
     2221 aatcgattcc aatagcggaa cagatattat cgtcaatagc agtacaagtt ttaataacgg
     2281 ttcatataat gtggctcttt atacctcttc agcaggaaac acagactacc atgcttctgg
     2341 tatcttatct ttccgaacag agaatcttga gatgagagag cagatccgcc cattgaatca
     2401 aaacgaagat caaatctacc actcgttgaa ctactattgg aatgaacgag agaaacaatc
     2461 agaaaatact cttggacata ccatatcaaa agattggatc gagtctctaa atacatgttc
     2521 gaaagaaggt cagcagcctg ttacgcgcta tgaaaatgga agtatccatg ttcatggact
     2581 gatgcagatt aaaccgggga atcgtcaaac agaaaaggaa agagtggatg gtcttccatt
     2641 atctgtaggt gctatttttg acggagaaac ctcaccgtct tcggaatatc cagagtatgc
     2701 aatcgtacaa gaaccaaatc agacaaaacc aaatgaacaa aataatccga ttattataca
     2761 agaagcaaat ggtgtggcgg agaatgcaga aggaaatatc attgaactga agccttttac
     2821 agtttttgtg tcaactttca caccaaatca aggaacgcct gtctttgaag atatgcgtta
     2881 tttcccagaa aagatccaaa tcgttgaaac acagtcaagt gctcaatttt ctaatcatac
     2941 cgctattttt tcagcacctt caaaaggaga atatacgcta gaagtcactt atcgttcgga
     3001 aatatttgat ggggaaaaat ggtctgctca tacagaatct gttacccagc aaaaagcgat
     3061 cactgtgcga gaaactgccg atgtgccccc gggaaatgac aaaccagaaa gagaaaatcc
     3121 aggaaatgaa tcaccaaatg gaaaaccaaa tggaaaacct tctcatccgg ataggacagc
     3181 aaaagaaagt gaaaaaaatg gcactcaagg actcccaaaa acgggagaat ttaataaccc
     3241 gttgctagca ttagctggag gaattttgtt aatcggtgta gttgtttatg tcatgaaaca
     3301 gcgaaaaaaa taagtggaat ttctcctttt atgatgtaaa acaattgact gaggaaagta
     3361 aaaatagaag gaaatgagac caatagatgt ctcacttcct tctatctttt tatagatgag
     3421 gttctccttg ctgcttttga ttggctgttc ttattacttg atgcaggaca gctttttcac
     3481 aatctctcca ctaaacggtg ttcaatcagc tgaccctcgg tattagctca acaaacggca
     3541 aaatatgagg aactattttt ccgttttttt ctctaatact caggtcagga cgctgatttc
     3601 acaacctctc gataaacggt gttcaaaagc cagtttctcg gtagtaagtc aaatttatgc
     3661 aaaaatgtgg aaagccattt tcacaaaatt tttcttacta cttgaaacta agcagctttt
     3721 tcacaacctc taatacatgg tattccagaa gtaacttctt cgaaaaataa gccgaaatgt
     3781 cacaaaaatt tgagaagcga ttttcccaaa ttccttctta tttcttgaag ctgaccactt
     3841 ctggcacaac ctcttaccag attcgcactc ttttttctgg cgctagatac atagcgtctt
     3901 gttccgtgat accaaatgtt tcatagaatt cgtcaaggtt ttttggttgc acgtttgctc
     3961 ggagtttagc aggggcatgg acatcgattt gcagtaaaag ttgttggtat tctttttttg
     4021 cttttgtacg ccaaatcgtt gcccaattga caaagaattc ctcgatagaa agatctgatt
     4081 ccgtttttgc tgcttccaat gcacagctta gaccgcctgc atcagcaata ttttccgaaa
     4141 cggttaattt tccgtttact tttccgtcag caaaaggcag accatcgaat tcttcgatca
     4201 ttgcttgtgc tttgttttga aattgtgtaa gatcttcttc tgtccaccag ttgttcaagt
     4261 tgccatattc gtcaaataaa gcgccgttat tatcaaatgc atgggaaatc tcatgagcga
     4321 taacagctcc gattccacca taattagcag agcttgattg ttctagactg taaaatggtg
     4381 cttgaaggat cgctgccgga aagacgatca cattccgaaa tggatgataa taagcattga
     4441 ccgtgtcagc actcatttcc cattctgaac gatccactgg tttgttccat ttgctgaaac
     4501 gatcttttaa tgtgagtttg ctgaaaacaa gagtatttga aagcaaagta ccaccctgat
     4561 ctgcaggtac tgttttgaac tggtcgaata atgctgggat cttatcgggg tagcctactt
     4621 ggatacctaa gttatttaat tttatgacgg ctttttcttt tgttgcatcc ccaagccagt
     4681 cattttcttc tagacgtttt ttataaacgg cgatcatttt tttcaccatt tgttctacgt
     4741 cttttttagc agcttcgcca aaatattttt ttccgtagta atcgccaact acttgatcga
     4801 attgtccaga ggcaaggtaa tacgctgctt ttttctgagg cattgcttcg tctgttccag
     4861 acaatacgcg agaatagatt ccgctgactt ggcggaattc ttctgataga taaccactaa
     4921 gagaacgtac tgttttaatg atcatccagc ttttgaataa cggaaaatga gcttctgtca
     4981 ggatgtcatt caaatgatcg aagtacactg gatcagttac gatcacttta tctggagttg
     5041 tcccaatcaa acccgtgaca agttttttca agtccagttt atctgtgtat ttaataaatg
     5101 tatcaaaaga ttgcggatta tacattttgc tgtaatctgc gttttcttct gcacttttta
     5161 catgaggagc aatcaattta tcaaattgca ctgcttgttc cgtgattgct tctgcctgac
     5221 ttttttcttt gccggcaagc tctaataact gaacagccat atcaaagaaa acgcctatta
     5281 gttgtgcacc gttgggatgg tctgatgcat aatacgtctt atctggaagg aataaagaag
     5341 gcggataagc aaagaaggcg ttcgtttgcg cattcttcat atctgcatct acatccagat
     5401 taaatggcaa tggtaatgta tccaacagcc attctggaaa ttgcttattc aattcgtcga
     5461 aagactgcaa tgattcaact ttttcgatta taggttgaag cggttgagca tgcaactcat
     5521 ttcttcggtc ataatcgttt gccagttgat aatatgcaag gaaatgtttc attttttctg
     5581 aagacacttt ttctggatgg gcaagcagat cgtctgtttc tcccatcaaa agttggtcga
     5641 tcccgtcaac taaatcttgg aaaccgcctg tagctggttt atctgcaggg atttttgctg
     5701 ttttttgcca ttcttcattg actgcttcat agaaatcttg tttcaatcgt tctttgttca
     5761 ttcatcgatc gctccttttt tgtttcgtgt gcccattatc ttacaaaatg aaggaattaa
     5821 caaatagaac ataaaaaatt gaaaattcga acaatcaaac tgatttgttt ttttcaatcc
     5881 ttcacttata atgaaaaaaa ggaggcgatc ccaactgaat caagtagtgg ataaaccttt
     5941 atatttcaaa gtagataaaa aaattaaaaa attagccagc actcagcagc tgcagtcaag
     6001 aaaaagtaaa ttgctttttt tagctctggt ttttgaagat caaagttatg tgattatcga
     6061 tcaatcagga catccggtag aatattctcc agcaaaatat acttatcaag agggtctttc
     6121 ttgtaaaaaa tggaagctga tcaatgaaga gccaatcgag ttatctagat ggatcaacag
     6181 aaaagaagat attcccgttt tgatcgaaga aaaaaggtca ggaaaggaac tcgcaaattg
     6241 ctgggtggga ttgccagaag aacgtttttt gaggtataag aaatgggcta ccccctcagg
     6301 ctatctttgt ggtacttacg cggcagcagt tctccttgcc tattatcaag actatcgtaa
     6361 ggggtggatg cttccaaatg agattcgcaa gaaaaatacg gctgattcgt tggttttgac
     6421 gaaggcattg cgtagccaga tccaaccact aggacttcct acgattcctt ttcaggttag
     6481 tacagggatt tctagttttt taaagaagaa tggcaatcat gagcgtgcac gagcaactct
     6541 tctaggttca tggcagcgag ctacaaaacg tatacgtgaa ggcaaacctg tgatgattgg
     6601 tattttgaaa gtacttggca gtacatatgg caatcattgg gtgacagcgt acgcttattt
     6661 tgaaactgaa acaggagaac gttattacaa agtacacgac aactggggcg attatcataa
     6721 agtcattcct gccagttgga gtaacggaac agtttcactt ccttaagaat ttgctgaaat
     6781 taacagaaaa tcttggaaaa atatgttaaa ctatcgaaga atagagaagt tttggggagg
     6841 actacatgag taataagtat caggatgaca cgttgaagat ttttagtttg aatggcaacc
     6901 gtccattagc tgaaaaaatc gcgaaagtgt tcggaacaga attaggtaaa agcgtcgtga
     6961 agcaatttag tgatggcgag atttccatca atatcgaaga aagtattcga ggggatcatg
     7021 tgtatatcgt tcaatcaacg aatgcacctg tcaatgatta ctatatggaa ctactgatca
     7081 tgatcgatgc aatgaagcga gcaagtgcga aaacgatcaa tgtcgtattg ccttactatg
     7141 ggtatgctag gcaagaccgc acggcgaaac ctcatgaacc gatcactgcg aaattgatcg
     7201 ctaacttgat cgaagaagca ggggcaactc gagtactgac tttagatctg cacacggttc
     7261 aagtacaagg attctttgat attcctgtag ataatttatt tacgatgccg ttgtttgctc
     7321 attattaccg tcaattaggt ctgacgggag atgacatcgt agttgtttcg ccaaaaaaca
     7381 gcggtgttca gcgtgcacgt agtctttcag aatatttgaa cagcacatta gccatcgtag
     7441 atcatgcaga tgatgcttca actgagggtg gttatgtgat cggtgatgtt caaggaaaga
     7501 catgtatcat ggtagatgat atcttgaaca caggagcaac gcttgcacga gcagcaaatg
     7561 tattaaaaga aaacggcgca aaagaagtat atgcctgtgc gtcacatgga ttattgtccg
     7621 atcatgcgaa aagtatttta gacaatgcac caatcaaaga tatttgtatc actgattctg
     7681 ttttcacaga agaaaatcgt catccagaaa acttaacaat cgttacatgt tcagaattaa
     7741 tgggtgaagc ggtcaaacgc atccatgaaa atacaccgat gagtccgtta ttccgtctgg
     7801 aagaaaaaga attcgaataa acaaaaaaaa gagggaaaga agttgtgaca gaagtgagca
     7861 gcttcaagga gctgcgacaa gacttttgtc acagctcctt ttttctatgc ccatgaaacg
     7921 tcctctagac aaaaatgagc tcttctttgg tttgttcgct attttatgct agaatgtgcc
     7981 tagcaataat ttggaaggga tgaatgaagt gaaaactatt tatctggatc atgcggcaac
     8041 tacccccatg catccacaag taattgaagc gatgacggca atcatgcaag acacgtttgg
     8101 aaatccttca agcatacatg gatttggtcg ccacgcgcat gaaaaattgg aaacagcaag
     8161 acaaatcatt gcagacagtc tgcaagcaaa acctcacgag atcatcttta acagtggggg
     8221 aacagaaggg gataatacag ctatcttaga aacagcgttc tcgcgtcaaa aagaaggcag
     8281 acatctaatc actacagcta tcgaacatcc tgctgttctt aataccatga aatatttaga
     8341 gacaaagggg tttgacgtta catatctgcc tgtagatgaa aaagggaatc tgtcgatcga
     8401 agaggtcaaa aaagcgttac gagaagatac gatcttggtc tctgtgatgt acgggaacaa
     8461 tgaaatcgga aatttgatgc caatcaaaga aatcggtgaa cttttagcgg atcatccagc
     8521 ttatttccat acagatgcgg tacaagcgta cggaaaccaa acgatccatc cagaagaatt
     8581 aggaatcgat ttattaagta tctctgctca taaaatcaat gggccaaaag gcgtcggttt
     8641 cttatataaa cgagacggag taaatatccc tgcattgctt ttaggcgggg agcaggaaga
     8701 aaagcggcgt gcaggtacag aaaatttagc agggatctgg ggtatggcaa aagcagttga
     8761 actgctgtca cctgaagaac gtgaagcacg taacaacaaa tataaaggat tccagcaatt
     8821 gattcttgat acattagaag caaacgaaat cccttatcaa gtaaatggtg atttaacgaa
     8881 taaactccct catgtgttga atcttcgctt aattggcgaa gcaaatgatt tgctactgat
     8941 gaagcttgat cttaatgggg tcgctatttc tactggttca gcttgtacag caggaaatat
     9001 cgagccttca cacgtattag aagcaatgta tggagaagat acaccgatac taaaagaatc
     9061 catccgtatc agctttggtt tgggaaatac cgaagacgac atccagtatt tccttgatca
     9121 attagttcgt gctgtcaaaa aataactagt gaagtcattc ttatatgaac agatgtctga
     9181 gagaacgcct tcatttttta gggaacttaa ctgaaaaacc tagtaaaaat caggataaac
     9241 ttttataata aagagaaact aattttatcg aggtgaaaat catggcattt gaaacaacag
     9301 caacggtact gggcgcacca attagctatc gtttgcatcc tgatgcaaaa agatatacat
     9361 tgcgcgataa tggatttaca gaaacaaacg gaggcaatta tcaattgatc cgaccattag
     9421 atgccacgcc gcaaagcaaa gaaggattca aattaaaaat cactgtatca aaagatattc
     9481 aaacattgaa aatgtcgatc actacagcaa atggattgaa agcagtcaat attttcaaag
     9541 atccaaaaca gaaaatgagc caagataaat tttattttct gatggatggg atgattagcc
     9601 gtgggttatt cgaaaaagtc gaatgatctg tggatataaa aagacaacta gttttgaaac
     9661 ggcctattgt tagatgacta actttgtaag gacgacgcaa attagttgtc ttttttattt
     9721 ttaaatttaa cacatgttgt tatttttaat attttgctga tttattgttt ctaagtaaag
     9781 aaatttaaat tgaaacgatt gttatttagg gaaaaaggta ttttttgaaa aaagaatccc
     9841 gtataatgat tgttgtgcaa acgctataca aaatgaaaaa gaaaagaggg atgtttatgg
     9901 aaaatgaaca gtcggtcgtt ttaacgaatt ggaagagaaa ttatttgttc tttttatcgg
     9961 gacagttttt gtcaggaatc actagtatgg tcgtccaata tgcgatcatc tggtatttga
    10021 caagagaaac cggttcggcg acaattttga gttttgctac tttactagga atgattccca
    10081 tggttttact aagcccattt gtaggaccgc tagtggatcg ctgggataaa aaagctcttt
    10141 tgattgtcac ggatattatt gtagcgattt tcgcgcttat tttagcagta gtcggaacga
    10201 tttcagaatc cttcccgatt tggctagttt ttgtgtcttt gtttatgcgt tcggtcgcac
    10261 aaacattcca aatgccaacg atccagtcga ttatgccgac aatcgttcca tcttctcaca
    10321 taacaaggac gaatggacag ttaggaatgg tccaatcagc gaatttcatc attgcgccag
    10381 ctctaggtgc ggctttgttt tcagtcgttc ctgtcaatta ccttatttta ttagatgtat
    10441 tgggggcagt ttttggtgtc ggcttattga tttttgtgaa gatcccaaaa gtttctcctg
    10501 aaatattgga agttccgctt actattttta aagatgcaaa atttgggctg cagcaattga
    10561 tggataataa agggttatgg tacataacga tcaatggtgc atttgtgatg ctgttgttca
    10621 tgccagcaat cagtctgtat ccattgatga cgctagatta ctttggtggt tccgttggac
    10681 aggcgggagc agtggaagta gtctacgcag tcggaatgct cttaggcggt gcactgatca
    10741 gttttatagg aacatggaaa gaccgaatga aacctattat tatagcctac atcattatgg
    10801 gcctgacgat cggtgcaagc ggattggttc caaacgatag tcaagggttc ttgtactttc
    10861 ttatcttgaa tgcaggagca ggttgtgcaa cgccatattt caatacactg ctgatggcaa
    10921 tgatccagca gagttatgaa tcgaatgttt taggtcgtgt cttagggaac ttcaactctt
    10981 tgatgaacct ggcaggtcca attggattat tatttgcagg accattagct gatcgtttag
    11041 gggtagaaaa aatgttcttg ttttccggaa tcgggatttt attatgcgga atcgttttat
    11101 ttttaacgtc agctgcccga aattatgata aagaattaca gaaaaaactg gtaaaagaac
    11161 accatgagca aaaagacgaa tagtatggaa tggagttgtg acagaagtct tgttacaact
    11221 ccatttctga tcctaatgtt cctgtggcaa taagcctact caaatgaaaa atatgagtaa
    11281 ttatctttat ttgtttaata cttgatatag ggcagttttt tcacgaccta tcgtacttcc
    11341 tctttgcttc atagatttct ttgatttgtt gctcagataa cggagtgcca aatggtagtt
    11401 tttcctttaa gagttctctt tccggtgcgt ctacgcctac attgacatcg ttctcgatag
    11461 gtacgctgta atgatggata tgtccatgaa gattgcggat agtgtcgtta ggcccgagca
    11521 acatcggata atgagtcaag taatattgtt gatggtcgaa tttaacgatg gcacctacat
    11581 cataaaaata aaatttggga cttccgtcag taagcctagg atcatttttt tctaaataac
    11641 ggaaaaaagc gcgactgtca tggttccctt tgatcaagtg gattgttcct tgcagttgag
    11701 tgaccagttc caaaatttct gcatttgctt tttcatattg cggatgcata gcaaggtcgc
    11761 ctagatgata aacatggtcg gtttctttta ttactgcatt ccagctagtg atcaactgtt
    11821 ggttcatttc atccacattt gcaaagagcc gcggagcaaa gtcgtttttt cctaataagc
    11881 gttcatggaa aaagtgcata tcactgataa agtaattcat gtatcttttg cctccctttt
    11941 ttcttagagt aaacaaaatc acttatctga gcaactcata acatctaaaa agaaaaggtt
    12001 gtaaatgaga cgtgcagctt taagcgataa gaagcaattt tgaaaaatag tttttcacaa
    12061 tttataaaat tattccttat tgccgaaagg ctggcccttt ttatgagtat gccaattttt
    12121 tatatcgaat gctcaaaaga tgagtgattt tttggttgac tatcatgttt gtgtactgaa
    12181 taggtccagt agaacagtat ttttacgact tgataattaa gggatgaaat tttcagaaat
    12241 atgacgtata atgtaaaata ctgaaaagtt gcgaccttca actcgtagat gtttcagaat
    12301 ctaattgaaa tgatggtgat atcatgacag acaacagcaa aacacgcgtt gtcgtaggga
    12361 tgagcggcgg tgtcgactca tctgtaacag cacttttgtt aaaggaacaa ggatacgacg
    12421 tgatcggaat attcatgaaa aattgggatg acacggacga aaacggtgta tgcacggcca
    12481 cggaagatta taaggatgta gcgaaagtag ctgctcaaat cgggattcct tattactctg
    12541 tgaattttga aaaagaatat tgggatcgtg tatttgaata tttcctagcc gaataccgcg
    12601 caggacgtac acctaaccca gacgtgatgt gcaacaaaga aatcaaattc aaagcattct
    12661 tggattatgc aatggatctt ggcgccgatt atgtagctac tggacattat gcgcaagtca
    12721 ctcgcgatga aaacggcgtg gtccacatgc ttcgtggagt agacaataat aaagatcaaa
    12781 cttacttcct tagccaatta tcacaagaac agctttcaaa aacgatgttc cctctaggcg
    12841 gcatggaaaa atcagaagta cgggcaattg ctgaacgcgc aggccttgcg actgctaaga
    12901 aaaaagattc aacgggaatc tgctttattg gtgaaaagaa cttcaaacaa tttttgagca
    12961 actatctgcc agcaaaaaaa ggaaatatgg tcactcttga tggtgaagtc aaaggacaac
    13021 atgctggctt gatgtattat acgattggtc aacgtcaagg actagggatc ggtggcggag
    13081 gagattcgca agaaccctgg tttgttgtag gaaaagatct agcgacaaac acattatatg
    13141 tggggcaagg gttccatcat cctgcattgt atgccacaag cttggatgca agtgaaatcc
    13201 actttacgac gaatgaacca atgccaaaag aattcaagtg tacagcgaaa ttccgttatc
    13261 gtcagcaaga cgttcctgtg acagttcgct tattggatga taatcgtgca gaagtcgtat
    13321 tcgacgaacc tgtacgtgca atcactcctg gacaagcagt tgtattttat gatggaatgg
    13381 aatgtctggg tggtggtttg atcgaccacg catatcaaga aacaaaagtt ttacaatacg
    13441 tttaagcgat attaaatgaa tcaaaacagc tatggctaac aaaagaaaca ccttttcttt
    13501 tggatagcat agctgttttt ttgcaataaa attaattttt attcatttta tacttgaaat
    13561 acttgtgtaa aaagttcata atatccaata atatgaataa agttgctttt tgttaagcaa
    13621 gagcagttgc aaaatagaag ggaatctttt gcaagtgatt tgttcaattt tataagtgag
    13681 ggtgatggaa atggaaaaga aatcgacggg tattagcaaa tggggactgg ttgctctggt
    13741 tgtcagttcg tcaatcggaa gcggtgtgtt tgggatcaca agtgatctgg ctagttctgc
    13801 agctccaggg ccagcgatcc tttcatggat aatcgttggg attggtattt tagctttggt
    13861 attatcgctg aatcatttag gagaaaagcg tccagatcta gatggtggta ttttcggtta
    13921 cgcggaagct tcatttggta aattaggtgg cttcatcagt ggttggggtt actggttgtc
    13981 tgcgtggctg ggaaatgttg cttttgcaac gatgctgatg agcgccatgg gagaattttt
    14041 cccgatattc aaaggtggac aaaatgttcc ttctatttta tttgcaagta tttttatttg
    14101 gggtctgact ttattagtaa acaatggagt tgaaggagcg agcgtcatca atacaatcgt
    14161 gacgatctgt aaattagtgc cattattcct atttttaata tttgtattta tcgcatttaa
    14221 agtgaatata ttcactgctg atttttgggg taacgtatct gataatctag tgaatggaac
    14281 agggcagcaa ggaacgatct ggttgcagat taaaggttgt ttgatggtta tgatgtgggt
    14341 ctttgtaggg atcgaaggcg caagtgtttt agccaaccgc gcgaagaaac gttcagaagc
    14401 ccagcaagca tcgatcattg gtctgctttg tttattgttt atctatatcc ttgcttcgat
    14461 cttgccatac ggcgtcttat ctcaagaaga gttagcaact atttcacaac ctgctatggc
    14521 aaatatttta aaaggaatcg tgggaaattg gggagcagcg ctgatcaata tcggcttgat
    14581 tatttcgatc attggttctt ggttgtcttg gacgatgctc ccagcagaaa caacgatgtt
    14641 gatggctcga gacggtctgt tgccgaaagg ttggggaaaa acgaataaaa aaaatgcccc
    14701 aacttattcg ttagttgtta ctgcaatctt gactaatcta ttcttattga ctttcttagt
    14761 tactgattat gcctatcaat ttgcctattc tttatgtaca gcggcaattt tgatttgtta
    14821 tctacttgtc gggatctatc aagtccagtt ttctttaaaa gaaggaatca aaaaacaagt
    14881 agtgatcggt gggattgctg tagtttttga attgatgggc atcattttag caggattttc
    14941 ttatgtcctt ttatgtagta ttgcctatct tcctggtttt tgcttctact ggatcgcttg
    15001 tcgtgaaacc aaacatcaaa ttacgggaaa agaaaaagta atgattgggc tgatttgtgt
    15061 ggccgcactg ctttcaatcg ggttgcttgc tgtaggctgg atcaagatat aagggggaaa
    15121 gaaacatgaa aatcgcaaat ttcggtgtag aagaatggct aaataaatgg gaaaagaaag
    15181 cggtatatga tattgcacaa agctcgatcg aagcattgac attagacgaa ctcgttggct
    15241 tagatgggac tagcgtaagt aaatttttcg aaaaacgcaa aaacgaacca ttggactatg
    15301 gctggatcga aggatcagat gttttcaaac aagaagtcgc ttctctttat caaaccgtcg
    15361 atccggaaaa catcttacaa acgaatggag cgactggtgc taatctttta gctctatacg
    15421 cgttagtgga agcaggagac catgttgttt cgatgcttcc tacttatcag caattatatg
    15481 acatccccaa atctttaggt gccgctgttg actttgttca tttgaaggaa gaagaagatt
    15541 ggcagtttga tttagaacag ctagaagcct tagtcaaacc tgaaacaaaa atcatctgtt
    15601 tgaatagtgc aaacaatcct acaggcacat tgctagatcg gaaaacttta gaaaaaatcg
    15661 cagaaattgc tcgtacagta gatgcatatg tattgataga tgaagtatat gcgcctttaa
    15721 cggataaagg tgaattcttg tcgatcgtag atgtctatga aaaaggtata gcaaccaatt
    15781 ccctatccaa aacctactcc attccaggta ttcgtattgg ctggacagct actggacctg
    15841 agttggcaga agtttttcga aagtatcgag actacacgat gatttgcggc ggagtcctgt
    15901 cagatgatct tgctgtacat gcattgaaga ataaagaaaa aatattggcg cgaaacaaaa
    15961 agatcattac agagaacctt gtgattttaa aacaatgggt agccaatgaa ccgaaagttg
    16021 aacttgtcgc tcccaattac gtctctactt ctttcattaa attagccatc gaagaagatg
    16081 accagacatt ttgtatcaat ttattagaag aaacaggagt actattagta ccaggaagtg
    16141 cctttgattt accgaagcat gcccgactag gttactgttg taaaaaagaa accttggaaa
    16201 agggattatt cctcttatct gagttcttga aaaaacaggc ctaaacaaaa acactgcaag
    16261 gacagtctgt gacaactgtt caagcagtgt ttttcattta cgcttattgt ctatgagaag
    16321 gagagtgccc tgaagctatg gagactcctg aagctttttc ttctgcagtt tcttgctgat
    16381 ggatctcttg tgtattgtgt cgtacaagca agaagttgac caaaacaaga agagaagtcg
    16441 aaagaaagac gccgcgataa ccaaatgcac tggaaacact ggagccgatc ataggcccta
    16501 ctacgttacc agttgcttgg aaagattggt tataactgaa gatccgtcca gcagcttgat
    16561 gaggcgaata ttttgtgatc aatgcttgga cagcagggag cagacaagca tcagagatac
    16621 caatcaaaaa acgtaaggca gctaattgcc aaacattcgt gacgaatgcc attgggatat
    16681 aaaccagaat cgcaaaaccc aaaccaatcg caagtattct ttcactccca atcttgtcac
    16741 ctaagcgacc aaatcttgga gcggctatca aagtggcaat cccaggtatc gaagcaatga
    16801 ttccgctgat caacgtaaca ttcccatgtc catgcagcaa ttggcgaatg tacagactga
    16861 tgattggact aatggaattg ttggatgctt ggatgatcat cgttgtgacg aacataccga
    16921 tgacgacgtg tggatatttc aagtctttga aaatctgttt ggcagaaacc atgttttctt
    16981 ttttcactgg gacaaatttt tcatggacga aaaacagact caacaagaag acgaaaaata
    17041 agatgatccc tgtaatgaaa aaggtagggc gatagccaaa cgctgaagca gagatcccgc
    17101 caagaagcgg tccaagtaat gttcctgtaa cagaaccagt tgctaaggtt caaagaactt
    17161 gaccactttt ttcttttggc gtcccagttg cgacaagggc agtggcatta ctgatataac
    17221 cagaaaaaat cccttgaagc agacgcaatg cgactaattg atacacacta gtcacgcaac
    17281 ccatcaaaga aatgacgatt gccattccta atgaagcacg caacagcatc aattttcttc
    17341 ctttttggtc ggctaatttt ccccaccaag gagagataag cgtagtcacc aagaaagtag
    17401 atgaaaaagt caatccgctc caaaagttca attgtgacgt actataatta cctaacgtat
    17461 ctatgtaaag tgacataaaa ggcatgacta aactaaatcc gataccagcc ataaatgttc
    17521 caaaccataa gacaaacaag ttttgttccc aagtttctct tttcctaaat aatttttcct
    17581 ttattcttgc agccaattag actccttctt tctgaaaatt ttcatctaca gctctttatt
    17641 ataaactaca atatgtagtt tatcaatgaa agtctctttt ttctctttct ttttcgtggt
    17701 aaactgaaag agtgaaggat ttattttatg agaggaaaca tggaaatgaa aaaaagaaat
    17761 ctgtctcaag agaaaatcat cgactgcttt cgagaactag cagaagagat ggatgttcag
    17821 caaataacct ttcagcattt agccaaacgc ttggatatca aggctccctc gctgtataac
    17881 catttcaaaa atattcgtga tgtcaaaaca gcgctaacag cgaaacttct gcaggaactt
    17941 aatgaccagc tgcgacgggc tcttgtggga aaaagtggtc aggaagcact gcagatctat
    18001 gcagaaactt atcgatcctt tgctttttca aatcaagcag tgtatgagtt actgatcagt
    18061 gtaccgcata ccaatgaagc tgttttatta gatggaatcc atgaaacgaa tcagatcatt
    18121 ttgcagatat ttgaggcttt tcaattaacg aaagaagaaa aagttcatcg aagcagggaa
    18181 cttcgtagta tgatccacgg ttatctttcc ttacgttttt taggatactt tacaaaagaa
    18241 ccaacaatcg atccagaaga cagctatttt tggatgatca ataactttat cgctactttg
    18301 ccttaaacaa tcagattcat ttgtaacaat tttaagatgt tcattaaatg acccttttat
    18361 aagagaaaat tcttgcaact ttcttgaaaa gtgactaaga tgagtctaga aaagctgaaa
    18421 gaaggagcac gagtatgtat caagtaatta ccatgttcgg ggacaatgag ccttggtggt
    18481 tttttgaaga ctgggaagaa gatattgaag aagaagaaac attcgagtct ttcgaagaag
    18541 cacgacaagc gtacgaaaaa caatggaacg aaatccatca aaattatgaa tatatcaatg
    18601 caaaaagtaa ctttctcagc gccttttgga atgacggaga tgagcgctgg tgtgaagagt
    18661 gtgacgatga tttacagcaa tacaagggac ttgcgttgtt gaaaaatcat cagcccatca
    18721 cagtagaaag cagaaaggaa ttttatgaaa caactaattc tagcggaaaa accaagcgtt
    18781 gcgaaagacc taagcaaggt gcttggtgct aatcaaaagc acaaaaatta ttatgaagga
    18841 cccaaggtga tcgttacttg ggcccttggt catttattag gattgaaaat gccggaagat
    18901 ctgaataagg aatggcagag ctggcaaatg gacacgttgc caatgatccc taaaaaacta
    18961 ggaatcaaac cacttcctaa aacaggacat caattaaaag caatcaagca gctggctcag
    19021 cgaaaagatg tctcagaagc agtcattgcg acagatgccg gaagagaagg ggaacttgtt
    19081 gctcgctgga tcttagagta tgtgcatttc aataaaccag tgaagcggtt atggatctct
    19141 tctcaaacag ataaagcaat caaaactgga ttcaagcaac taagacctgc gaaagaatat
    19201 gatgcattgt atgaatctgc tcttgcccgg gcaaaagcgg actggctggt aggattgaat
    19261 gtgaccagag ccttaactgt gaagtacgcg gataatttat cagcaggccg tgtgcagaca
    19321 ccgacactag cattagtgag agaccaagaa aagaaaatcg agcagttccg tccacaaacg
    19381 tatttcacga tcacgttgca agctggaaaa gaacaagcca aaatgcttca aaaaaatcaa
    19441 tttgctttaa aaacacaaga agaagcacaa aaacttgttc aattgatcag tacaagcaaa
    19501 ggaactgtca cagaagtcca agaaaaatta aaaacggaat ctgcgccatt gccttatgat
    19561 ttaacagaga tccaaagaga agccaatcaa cgctatggct attctgcaaa gaaaacatta
    19621 agtcttgtac aaagtttgta tgagacccat aagatcgtta cttatccgcg tacagacagc
    19681 aaatatctga caaatgacat gaaaagtacg atgaaagaac gattgcaagc agtcagcgac
    19741 tttgctcctg aggtcaagca gtatttgaaa aacggagcca tcgtccaaca gacaaaagta
    19801 ttccaagaca gtaaagtgac cgatcaccat gcacttttgc caactgaaaa cagagcacgt
    19861 tatgaaaaat taagtaatga agagcgaaaa atctatcaaa tgattgtctc tcgtttttta
    19921 ggactgtttg ctcaacccca taaagtcagc caaacaaaag ttacggttga atttggtaaa
    19981 gaacgattca tttttcggca aaatcgtgtc gtccaagcag gatggaaagg cgaaactgaa
    20041 actgagacgg aaacagtcaa atgggaaaaa ggaatgagaa tcaatcccga tttcgccatc
    20101 aaaaaagaac tatctgcacc gcctaaacca ttgtcagaag caagcttgct tggtcaaatg
    20161 gaaaaataca gtctgggaac cccagctacg cgtgcagaga ttatcgagaa actaatcaaa
    20221 tctgaactga tggaacgtac ccctcaagga ctgcaagtat ctcctaaagg taagcaatta
    20281 ttagtattgg taaatccctc tttagtaaca ccagatttga cagaaagctg ggaaaaagac
    20341 cttgaagcta ttgctgcagg aaagaaacaa gcaggaacct tcttgaatgg aatcgaaaaa
    20401 gaaacaaaac gcttggtgaa tgagatcaaa tcaagcaaac aagaatatca agatttctcg
    20461 attacccaaa agaaatgccc agaatgtggg gccaatctgc gcgagaaaaa cacacgtgac
    20521 ggcaaaatct atgtctgcac caattcagat tgcaactacc gcagaagaaa agatccaaaa
    20581 gtctctaatc atcgatgccc gcaatgtcac cggaaaatgg aaatcatcga aggtaaaaac
    20641 ggtgcttatt tccgttgtaa atacgatgga acgacagaaa aattgcttga taaaaaagag
    20701 cagaagaaga aaatgaccaa acatgaagaa cgacgcttgc tgaaaaaata tgcacaagca
    20761 gaagaaccag aagaaagccc attagcagca gcactaaaag cagcaatgaa gaagtaataa
    20821 gatatgaaag aggttgtgat agaagtggtc agcttcaaga aataagaagg aatttacgaa
    20881 aattgcttct caaatttttg tgaaatttcg gcttatttcc gaagaagcta cttctggaac
    20941 accatttatt cggaaaaaag gactgtgaca agacttttgt cacagtcctt tttcatttaa
    21001 aaaaatgttt acttcatttg acgtatatta ttggtgaggc ttcaattcat ttggcttcac
    21061 tttttctgtt tctcttatga tataaacagg acgctgtttc gtttctaaga agattttccc
    21121 aatatatttc ccgatgattc caagacataa caattggatt ccaccaatga acaggaagat
    21181 cgagaccatg gaaggccatc ctgatgtagg atcaccaaac aacaatgccc gcaaaataat
    21241 gaacaaaatt gcaataccag aaccgacaca agacaaagcg ccgataaatg aagcaatcgt
    21301 taacggtgca tccgagaagt tcacgatacc atcaatcgag tagttgaata atttccagaa
    21361 ggaccaagat gtttcgccag ccacacgttc gcggttttca taagaaatat attctgtttt
    21421 aaatccgacc cagctaaata atccttttga aaatcggttg tattctgtta attctaggac
    21481 ggcatccacc atttgacgag tcatcaagcg gaaatcacga gcgccatcga ccatttctgt
    21541 atcgctgatt ttattgatca actggtagaa ttttttcgca aagaaactgc gtatcgcagg
    21601 ctcgccttca cgagtcgaac gccgggtgcc gacacaatcg atatcagaag tagtgatccg
    21661 gttgatcatc tcaggaagca gttcgggcgg atcttgcaga tcaacatcca tcacggtcac
    21721 taaatctcct gtagaagctt gcaatccagc gtacagagcc gcttctttcc caaaatttct
    21781 ggaaaatgag acaaagcgga cataaggata agctgaggct agcttcttga ttgctgctaa
    21841 cgtaccgtct tttgatccgt cgtcaataaa caaatattcg aactcatggg cacctttgaa
    21901 tttttcaacc tcatcaaaaa aaagaggaac agttttttct tcattaaaac atgggacaac
    21961 aattgatact ttcatcgttt ctatcctttc aatcgtatgt ttatatatgg tttacattca
    22021 aaatttaata aaccttaaat aacaccttta aaatttgatt ttacgtaaaa gaacaggtat
    22081 tctttttgta aactaacgta tttagtttac catgtttcct cggtttatca aacttttaag
    22141 aacaattttt attagttggc aagtagtttt ggatagagaa aaaggaggaa gttccagtct
    22201 attgactagg agcaaacaaa atgaaaatac acacacctat cggcttttta tggttagatg
    22261 cagatgaatc cggattgact gctgtatctt ttcagccaat ttcagaaagc gaatcagcga
    22321 atcagcgaat tttaacgcgc acacaagaac agtttgaaga atattttgcg ggaaaaagaa
    22381 aagaatttga tgttccttta tctataaaga gaggtacgcc ttttcagcaa aatgtctgga
    22441 aagctttgtc gaccattcct tatggagaaa tccgaacgta taaagaagta gccgttgctg
    22501 ttgatagtcc gaaagctgtg cgagcgattg ggcaggcaaa tcgaaccaat ccattgccaa
    22561 tcgttattcc ttgtcatcga gtgatcgggc aaaatggaaa actgacagga tatatgggaa
    22621 acgcaataga tggggttgaa atcaaacgcc aattgttgtt gttagaagga tatctgcaaa
    22681 attaaaaaca gttgtgattc ggacattgtt ccatgcgaga atcacaactg ttttttcatg
    22741 ttcattctaa actttgatca gccaaataaa tagtaaagcc atcactgttt ggagaagccc
    22801 gacagctaat ccataagata agaagcgtgt accttctttc atgaattttg caaagtctaa
    22861 acgcagacca attgccgcta aagcagtgat ctcaaaccag ctgctgataa aatgtgccga
    22921 ttggtcaata actacaggta aagaaaacaa gctgttgacc aaacaaaaga tcacaaaccc
    22981 taatacatac caaggaagcg gtaattttcg ttttttctgc gtatcagttg gcgtttcaaa
    23041 cagagctggt tgtttcttgt gcatgaattt ttcaaacaga tagacaacaa caacgagcat
    23101 cataatacgt agaatcttaa atagcatggc gaattcgaca acagaattgt caagtaagct
    23161 tgccccagcg actacttgac caacagattg gagtgtacca cccaaaagtg cacttttttc
    23221 aatgagattt ccaccaaaca actgtaatcc aagaaaaggg agagtcaaca tcatcacagt
    23281 acccagtaga ttgactagtg tgatgatctg tcctttttct tcctctttcg catggatgga
    23341 aggggcgatt gaagcaatag cagaagaacc acaaacagcg tttcctccag ccatcatcaa
    23401 agaagaagta tcgctgaatt gcagtttttt accaatcaag taagcaccga tgatagtgac
    23461 tgacatttgc agtaacacaa aaacaattcc ttttacacca atttgagcaa tcgtttgagc
    23521 tgtgacagtt gcacctagta aaaaaacaga gaattctaat agtttgcttt cagcaaattt
    23581 tgtgccgatt gctagaacag gctgtttaaa aaaagtattt cctaataaaa tacccaataa
    23641 aatagcaata gtcgctccac ctaactgtgg cagataaata gcaagaagcc tgctgaaggc
    23701 agcgatggca aaagaaagca gtaaaccggg taagatttcg cggaccgctg aatcctttac
    23761 agattttatt ggatgtgaca atgatttcat ttgatcggtt cctttcgtca ataaatacta
    23821 aaagcatact cgaacatagt agcactaaac cagtattttg acaaaagaac aagaggtcgt
    23881 gacaaaagtc ttgtacgacc tcttgtttcg aataaacggt gttccagaag tagcttctac
    23941 ggaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    24001 tcttgaagct gaccacttct tttacgacct cttggtaaac ggtgttcaaa agcttgcatc
    24061 tgcggtaata agcccaacca gtacaaaaac atgaacagct gtttttgttt ggccgttctt
    24121 attacttgat gcagaacagc tttttcacaa cctattgaat cgattattaa gcgatttttt
    24181 tagaaacaaa ttttccatgt ttgctatgtt ctttaattaa gaaacgtacg taatgagcaa
    24241 gaacgtagaa gacaactaca cttgcaaaca gacattccgc aacttttaag aaatccaata
    24301 cgctactata gatacctaaa ctgttgaagt aatgaacagt agtaaatata gctgttgcag
    24361 agataacaag cgggaatgta aaggctgcat agcttggata aaaacgaagc ttgaacaaac
    24421 gtggaagcat agacagcaca acgaaataca atacttgaga taaagcgaac aatgctacga
    24481 caagataaaa attaggttca gcaaagtttt taagataacc tgttaggcaa agagaaccag
    24541 gtgctgcaac gatcgtaatc agcggacgtg tcggatgagc catatttctt acgaaaaaga
    24601 cacgtttgat gatgatcggc aataagaacg catagcagat caatgaaatc caaaagaaaa
    24661 tttttccaat aagtgggaag aagttcccgg cagttacagt gatgacccca aatccaacgt
    24721 aaatgataaa ccagcttgga taaattgctt caattgaaag atcagctttg ataacatggt
    24781 gataattgaa ataaaagaca aggatcactt ggaagataac agcgcacagc caaatgaatt
    24841 tgacaattgg tgcaatactt tcatatgaaa caaaataagt acaaataacc atgagtgaca
    24901 tcgtaaaagt tggtgaaacg gaggcgatga ttggatcatg caacgtttgt ttcgtgtgtt
    24961 cgaataagat aaacaattta acaaaaatga atatcataag aatcatagag ataccgccag
    25021 ttagattgcc taaagcaaca tggtgatagt ctttaaacag atttccaagg gaagctaacc
    25081 caaggatcaa cccgcagatc gggatcggaa taattttcaa aaagttcttc atatataata
    25141 ttctcctttt tatttaatag attttagtac caagatgaac gaatacattg taaaatgaat
    25201 aggaatgtat gattacgagc aagcagacag ctgattgtct tcatctaagt tactagttgg
    25261 atcagcaact gttttgttgt acatttctat tatagatggt tttttgtata tttaaatcaa
    25321 atagttttta tagtaattat cataaaaagt tatggaagtg gtgccatgtt taagttatta
    25381 acgacttttc gagcagtata tgaaacaaga aatttttcca gagcagcaga agtactgttt
    25441 ttatctcaac cagcagtctc gaatcaaatc aaacagttgg agcgggaatt gaatatcgat
    25501 ctttttcaac gtaacggaag aaaagagatg cttccaacta agcaagccga cttattatat
    25561 gaacggacac tggttttgtt agaagaatgg gaagaaactc agcaaaaatt gctcaatgaa
    25621 caaaatgaag aagaagtatg tcggatcgtt gcttcccaca cctttgctgt ctatcttttg
    25681 cctcagttga tgaaacatct gatcaaaagg tttcctaaag tgcattttga gatcgaaatc
    25741 acgaattctc atgaatcatt agaacaagta gcgaaacatg aatccgattt tggttttatc
    25801 gaaaagccat tagagacttc cggcgtacaa agatactctt tgatggaaga ccagctagtc
    25861 atcgctgggg acccaaaaag caaattgtgg cttgtaagag aagcaaactc tggtgttttt
    25921 cattacacgc agcgttattt tgaagagatg aatatccaag ataagaagat gatcgtgaaa
    25981 agtaacgatg taattgttgc gttgctgaag gaaggaatcg gtcgctcgat tttgtctaag
    26041 cgctctgttc cagaaaatgt gccaatgact ccgttgaatt tccatcgata tttttatttc
    26101 atccaacgaa ccaatctggt ttctgatcag ctgctagaga ttgccgaaga gatccttcga
    26161 tacaatgaaa gcacagaaac ttctcacagc gaggagaatc tcagctggta gataattgca
    26221 tgtatcaaaa aaagttctat gatacactaa agataagcag taggcttttg tactgcattt
    26281 gctcaagcaa ctgcacaaac ggaaagttaa tttttcccaa atgtctatgg aaagactttc
    26341 cattatctaa ctggaccgct ataataatca gcagtatggt ttttaagaaa agggatgaaa
    26401 catggaaaaa aaattaaaag cttcgaacga taatcgtatt gattatggcg tgattttacc
    26461 agtctttctg ttatgtttga ttggtctgct ctcattatat gtagcactta gccatgatcc
    26521 gaacaatcca gcagtcttga aaggcgtagg gatgcaagtt gtctggtatt tagttggagc
    26581 ggcagcaatc gttgtcatca tgcatttgaa ttccaaatgg atctggaaac tgactcctta
    26641 cctttatggt ttggggttag tggtaatggg actcttgctc aaattctatg atcagaattt
    26701 agctgcttca acagggtcta aaaactggtt tcgattcggt gcgtttactt tccagccagc
    26761 agagttgatg aagattgcgt acatcttgat gatggcactg atcgtaacta aacataatac
    26821 gcaagtaaaa gaacggacga tgaaatcgga cttttggctg attgggaagc tcttgatcgt
    26881 gacaataccg gttttagcat tgatcatggc ccaagatgac tttggaacga tgcttgtctt
    26941 tcttgcgatc tttggcggga ttttcctgat gtctggaatc tcttggcgca tcatcgcacc
    27001 tgtagtaatc ttggcagtag tcgttggagc aggaacgatc ttcttggtta cgactgaagg
    27061 cggaagagac ttgctttata aagttggatt caaatcttat cagtttgcac gtattgattc
    27121 atggctggac cctttccatg atacgtcagg aatgagttat cagccggccc aaggactttt
    27181 ggcaattggt accggtggct tgtttggtaa aggattcaac gtaagcaata tctatgtgcc
    27241 tgttcgtgag tccgatatga tttttaccgt tatcggtgaa aacttcggct ttatcggtgg
    27301 tgcattcgtc attttcttgt attttatttt aatctatcgt atgattcggg tgtgtttcga
    27361 tacgaataat gaattttacg cctatatcgc aagcggtttg atcatgatgc tgctgttcca
    27421 tgtctttgag aatattggag caaatatcgg attacttcct ttaacaggga tcccattgcc
    27481 tttcattagt cagggaggat cctcgatttt agggaacatg atcggtatcg ggttgatatt
    27541 atcgatgaga taccaaaatg aagcacctgc aagatcttca aggaggtaag aaaattgtac
    27601 accttttact ggtacccgaa atgttcaaca tgcaaaaaag ccaaagcgtg gttagaccag
    27661 catggcgtta gttatcaaac cgttgatatg atcaaagaag taccgaaggc ctcaacgatt
    27721 gagacttggc tagaaacgag tcaagtacca atccgtcgcc tgttcaacac aagcgggatg
    27781 aaatatcggg aacttggact gaaagatcaa gtaggcgagt ttacggcttc aaaagcgagc
    27841 caagtcttat cgacagatgg tatgctgatc aagcgaccac tgatcttacg agaggaccag
    27901 cttgttgcaa ttggattcaa tgaaaaaatt tatgaaggag aactgatata aaatggcagg
    27961 caagcatttg aagaaaaagg acaacctttg gatcttattt aatggaaaag aatactgtgt
    28021 tggtttgaca aatgaagcgc aagaagaact tggagacatc acgtttgcca atctgccaaa
    28081 agcaggacaa tcgctaaaaa aagatgaacc tctgatcgaa gttgaagcgg aaaaagcggt
    28141 caatgaattt gccagcccgc taacgggtac agtttcttct gttaatgata aaatcggtga
    28201 agatatcaat atcttgaatg attcagatga aatgaatgcg tggatcttaa gtttcaaaga
    28261 tgttgatcca aaagaatttg acgaattata aaatcagaaa atcacttgaa aaaagttgac
    28321 actgattctt ccagttgcta taatcataag caatcctata tgaaacgaga aagctttgaa
    28381 aagaagagta catgtcacgg acgtttgaag agagccctgt ttgctgaaaa tgggtaacga
    28441 aaatgagatg gaagatggtc ttagagcaga gtaagcgagc gtaagcaaac ttactacggg
    28501 agtgcccgtt acagcgcgac agtatacgga aagaagctaa gtccaagtac tggaagaggc
    28561 tgttattgcg aagtaacggt aaatcaaggt ggtaacacgg aagttcaagc tttcgtcctt
    28621 ttgcttaagg caagagggcg aaggcttttt tgtttgaaaa attataagag aaagatgtga
    28681 atggcatgtc attaatccaa ctaacgaatg tcaaaaagac attcaatggg aaaaacggaa
    28741 acatccaagc cgtcaatgac gtttcactaa acgttgaaaa aggggatatt tatggaatcg
    28801 tcggctattc tggcgcagga aaaagtacat tagtccgtct cctgaatgga cttgagcttc
    28861 ccacgagcgg cgaagtgatc gtaaacgatc aagatattac aaaattaaaa aacaaagaat
    28921 tacgtacatt ccgaaagaaa atcggtatga tcttccagca tttcaacctg ttatggtcaa
    28981 gaactgtact tgaaaatatc cagctgccgc tggaattagc tggcgtacca aagagcaaac
    29041 gtaaagaacg ggcagaagaa ctgttgcgtt tagtcgggtt agaaggacgc ggacaagcat
    29101 atccttcaca actttccggc ggacagaaac aacgcgtcgg tatcgcacga gcattggcaa
    29161 atgatcctga gatcctgctt tgtgatgagg ctactagcgc attggacccg caaacaacag
    29221 aagaagtatt agacttattg ttagcgatca ataagaaatt gaatttgacc atcgtcttga
    29281 tcacacatga aatgaatgtg atccgcaaaa tctgcaacaa agtcgctgtg atggagttgg
    29341 ggaaagtcgt agaagaagga gacgtattga ctgtcttcag acaaccgaaa aaagaagtaa
    29401 ccaaacgctt tgtacaacaa gacatcgaac cagaagctga ttcgacagaa ctattagctg
    29461 aactcatcaa agaaaatcca agcggccgtt tggcaacttt gacctttagc gaaagaaatg
    29521 ccaacgagcc agtcatttcc caagcgattc gacgctataa catcgatgtc aacgttgtgt
    29581 atggaaagat caaacaagtg aaagaaggtt cctttggttc attgactgtc ttgatgactg
    29641 gtgaaaccaa agaattagat caagcagaag tgttcatcaa agaacaagga gtaggaattg
    29701 aggtgatcca tcgtggataa aagcttttca gaaacatatc tagattttaa acaaatcaac
    29761 ccggaaacga tgaatcaggc tatccttgat acactatata tgacattaat ctcaatggtc
    29821 ttagtgacga tcatcggatt agttttggga ttgatccttt actctattgg acgcaaaaac
    29881 aaaccgtatg ctcgaatcat ttatggaatc gtatcgatca tcagtaatat tttccggtca
    29941 acgccattta tgatcctgat ggttttgatc attccattta ctaaagcaat cgtaggcact
    30001 atgctaggtg cgcaagcagc cattccagct cttgttttat ccgcagcacc attttatgcc
    30061 cgtctagtag agattgcttt tcgtgaagtc agccctggtg tactggaagc agcagatgca
    30121 atgggagcaa gttactggga aatcatctgg aaggtcttga tacctgaaag tattcccgca
    30181 ttgatttctg gattaacagt tacaacgatc tcgatgatcg gatttactgc gatggccggc
    30241 gcgatcggcg caggtggact tggaggtctt gcttggcagg aaggctacca gcgtggtaat
    30301 ctaacagtga cattcgttgc gactttaatt attttactaa tcgtatttat cgttcaagga
    30361 atcggtgatt tcttaactaa gaaaacagac aaaagataat aggatatagg gggatgtagg
    30421 atatgaaaaa gaaaattttt ggatttgcag cagcgctttt gctgactgta ggattagcag
    30481 catgcggtaa caacagcgac agcaaagatt cagcaaataa agcagacgac acgacattga
    30541 aagtcggcgc ttcaccgaca ccgcacgcag agattttgga acatgtgaaa ccattattga
    30601 aagatgaagg aatcgatctt gaaatcgtca aattcgatga ctatgtatta ccgaaccaat
    30661 ctttggaaga aggcgacatt gatgccaatt acttccagca tattccttat ttgaacaaag
    30721 aaatcaaaga aaaaggatat gactttgtca atgccggtgc tgtccacatc gaaccaatgg
    30781 gcttgtactc taaaaaagtc aaagatgttt ccgaattaaa agatggtgca acaatcatta
    30841 cttcaaattc tgaatcagac tggggacgca tcatcacaat tctacaagat gctggcttag
    30901 tcaaagtcaa agatggagta gatctggaaa cagcaacatt cgatgatatt gaagaaaacc
    30961 ctaagaactt gaagttcaac catacgatcg acccatcact tttagcttct acgtaccaaa
    31021 atgatgaagg tgacctagtt gcaatcaacg caaacttcgc ttacggcgca gggttgaatc
    31081 cattagaaga tgccgtattg cttgaaaagg acaactctcc ttacgtgaat attattgcga
    31141 ctcgtaaagg tgatgaagac agtgacaaaa tcaaaaaact gattgaagtg cttcaccgca
    31201 aagaagtcca agaatggatc acagacaaat ggggcggctc tgttaaacca gtaaaggctg
    31261 atgcaaaata aggtatggaa caaaaaagat gagaaataag tctgtaatgg cttatttctc
    31321 atctttttta tacctattct aatttagaaa tcaaaattgt ttaataataa ataaaagata
    31381 tttgatttta gtaaggaagg tcattgcgct tcattctcaa ttagtctaaa atgagtgaga
    31441 acgaaagaga atttgggggt ataatgcatg tctgtcttag aaataaaaaa tctacatgtg
    31501 tcgatcgagg aaaaagaaat ccttaaaggc gtaaacctaa caatgaaaac cggagaaatc
    31561 catgccatca tgggacctaa cggcacagga aaatctacct tatctgccgc tatcatgggc
    31621 aatccaaact atgaagtaac agaaggcgaa attttgtttg acgggaaaaa tgttttagac
    31681 ttggaagtag atgaacgtgc gcgacttggc ttatttttag ctatgcaata tccaagtgag
    31741 ataccaggta tcacgaatgc ggaattcatg cgtgcagcga tcaatgcgaa acgtgatgaa
    31801 aacgacaaaa tttctgttat ggcatttttg aaaaagttag atgaaaaaat ggctttacta
    31861 aatatgccag aagaaatggc tgaacgttat ctgaacgaag gtttttctgg cggagaaaag
    31921 aaacggaatg agatcctgca attgttgatg ttagaaccta catttgcaat tttagacgaa
    31981 atcgattcag gtctggatat cgatgctttg aaagtcgttg ctaaaggagt aaatgaaatg
    32041 cgcggagaca atttcggtgc attgatcatc acccactatc aacgattatt gaactatatc
    32101 acacctgatg tcgttcacat catgatggaa ggccgagtag tcatgacagg gaatgctgac
    32161 ctagctaaac gtctggaagc agaaggctac gctggtatca gtaaagaatt aggaatcgac
    32221 tataaagaag aagaagcgta agggggagca agaatgaaag aaaaaaactg gctcgattat
    32281 cttgacgcgg taaatgattt ttctttaagt aaaggcgaac ctgattggat gagaaccttc
    32341 cgtcaagacg cactagcgaa agcagatgaa ctgccattgc cgcatatcga tcgggtgaaa
    32401 ttccatcgct ggtcgttatt tgatgtgaaa gaaacacaaa ccatcagtga aacaggcacg
    32461 attccggcat ttgatgcaat gaaagacaat cctgtgcttg tccaacaagg ttcatggacg
    32521 atttttgaac aactgcctgt ggaattagca gaaaaaggag tcatttttac cgacttgttc
    32581 actgcaatga tcgaatatcc agaactagtc caagaatatt atatgaaaaa agcagtaaac
    32641 atgaacgaag accaattgac tgcactccat gtagctttta tgaacagcgg tattttcctg
    32701 tatgtgccta aaaatgtcgt gatcgatgaa ccactcgagt ccttattcat tcaagatggc
    32761 gcatccgatg agcacttctt caagcatgtg ttgatcgtgg cagatgaaca tagtgaattt
    32821 tcttatctgg aacgtttcca gacaaccaaa gaacaagtag caaaatcatc aggtaatatc
    32881 atcgtggaag tcatcgctaa agctggttca aaaatcaaat attcagccgt agatcaatta
    32941 ggagaaaata ttacttctta tatgaatcgc cgtgggcaca ttttacgaga tgcttctgtc
    33001 gactgggcaa ttggtgtcat gaatgacggt catgtgatcg ctgactttga ctcagattta
    33061 gcaggagaag gtgcacacgc agaagtcaaa atcgtggcaa tcagctcggg cagacaaatc
    33121 caaggaatcg atacacgagt aaccaataaa gcccctcata cgatcgggca tattttgcaa
    33181 cacggagtga tccgagaaaa aggaacattg acctttaatg gtatcggcca catcttgaaa
    33241 ggggcaaaag gtgcagatgc ccaacaagaa agtcgtgtct tgatgctttc tgataaagca
    33301 cgtggtgatg cgaatcctat tcttttgatc gatgaaaacg aagtaactgc tggacatgct
    33361 gccagtgtag gacgagttga tccagaagaa atgtactatc tgatgagtag aggattgcat
    33421 aaagaagaag cagagcgctt agtgattcga ggattcctcg gttctgtctt aacagcaatc
    33481 cctgtagaac aagttcggaa agaattagtc gaagtaatcg aagggaagtt gaatggatga
    33541 tcgatgctaa aaagctacgc aatgattttc ctatcttatt tcaagacgtc aatgatgaac
    33601 ctttagttta tctggataat gcagcgacga cccaaaagcc aaaacaagta ctccaggcat
    33661 tagcgtacta ttacgaacac gataacgcca atgttcatcg cggtgtccat acgttagcag
    33721 aacgcgcgac ccatgattat gaacaagcaa gagaaaaagt acgggcgttc atccatgcaa
    33781 aagaaacagc agaagtgctg tttactcgtg gtacgacgac aagcttgaat tgggtagcca
    33841 gaaccttcgg cgaccgctat atcaaagcag gagacgagat agtgctctct tacatggaac
    33901 atcattctaa tgttatcccg tggcagcagt tggcaaaacg aacaggtgct gtcttgaaat
    33961 atatcgaact aacagaagaa ggcttcctgg atatggagga tgcagcgaaa aagattacgg
    34021 acaaaaccaa aatcgtctcc attgctcatg tatccaatgt cttaggtgtc ttcaatcctg
    34081 ttcaagaatt agctgaactt gtccatgaaa acggcggggt catcgtagta gatggtgctc
    34141 aatctgcccc gcacatggcc attgatgtcc aagcactaga ctgtgatttc tttgctttca
    34201 gcggacacaa aatgtgcgga ccaacaggta ttggtgtttt atatggtaaa cgaaaatggt
    34261 tagaagaaat ggaacctgtt gaatttggtg gcgaaatgat cgattttgtc gaattatacg
    34321 acagtacatg gaaagaacta ccttggaaat tcgaagcagg gacaccaaat atcgccggcg
    34381 ctatcgcatt aggccatgca atcgattatt tgcaaaaact cggtatggac aatatccatc
    34441 gatatgaaga agaactagcc gcttatgtgt tgccaaaatt acaagcaatt gatggattga
    34501 ctgtttatgg tccacaagac ccgaattttc ggacaggagt cgttgcattc aatttggatg
    34561 ggttacatcc ccatgatgtc gcgacagctt tagacatgga aggggtagcc gtcagagcag
    34621 gtcatcattg tgcgcaaccg ctattgaagt accttgaagt atcagcgact gctcgcgcga
    34681 gcttttattt ctacaacaca acgcaagacg ccgaccgttt agttgaagca atcatggcaa
    34741 caaaggagtt tttccaacat gggactatct aaattagaca atttataccg tcaagtgatc
    34801 ttggatcatt cttcccatcc gcatcatcgt ggaagcttga ctgactcgac aagcaagatc
    34861 gaattgaaca accccacatg tggagatgtg atcgaattgc aagtcgatgt aaaagacgga
    34921 atcatcaaaa atatcgcctt tgacggcagt ggctgctcca tcagtacggc aagtgcctcg
    34981 atgatgacag acgccgtcat cggaaaatca atagaagaag cagaacattt aactgtagac
    35041 tttctacagc ttgtccaagg aaaagaagtc aaagatcccg accaattagg cgatgctgct
    35101 atgcttggtg gtgtagctaa atttcccgct cggatcaaat gtgcgacact tgcctggaaa
    35161 gcactaggaa aagcaatgga aaacgatggt accgcagctt tccatcatta ccacaaggaa
    35221 gaggagtgag aacatgggcg taccagaatt agaagaatat aaattcgggt ttcacgatga
    35281 tgtcgaaccg gttttcagta caggagaagg cttgacagaa gaagtgatcc gtgagatgtc
    35341 aagaatcaaa ggcgaaccag aatggatgct tgaattccgt ctgaaatctc tagaaacatt
    35401 taacaaaatg ccaatgcaaa catggggccc cgacctttca gacatcgatt tcgatgccat
    35461 caaatattac caaaaaccaa gtgacaaacc cgctcgtgac tgggaagacg taccagaaaa
    35521 aatcaaagat acatttgaaa agattggtat accagaagct gaacgagctt atctagccgg
    35581 tgcttccgca caatatgaat cagaagtcgt gtaccataat atgaaagatg aattcgaaaa
    35641 gttaggaatc gtctttactg acactgactc tgctttgaaa gaataccctg aattattcaa
    35701 agagtacttt tcaaaacttg taccgccaac cgacaacaaa ttagcagcgc tgaattcagc
    35761 cgtatggtca ggtgggacct ttatttacgt gccaaaaggc gtcaaagtcg atgttccatt
    35821 acaaacgtac ttccggatca atgcggaaaa cacggggcaa ttcgaacgta cgctgatcat
    35881 tgttgacgaa ggagcaagca tccactatgt cgaaggttgt acggcaccta cttattcaag
    35941 taacagcctg catgccgcca tcgtagaaat ctttacaaga aaagatgcgt attgccgtta
    36001 cacaacgatc caaaactggt cagacaatgt gtacaactta gtaaccaaac gagcaaaagc
    36061 atacgaaggc gcaaccgttg aatggatcga cggaaaccta ggtgcaaaaa caacgatgaa
    36121 atacccaagt gtttacttag atggaaaagg tgcacgaggc accatgctct ccatcgcctt
    36181 tgcaggagca aaccaaatcc aagatacggg tgcaaaaatg atccacaatg caccaaatac
    36241 atctagctct atcgtctcta aatcaatttc aaaagacggc ggagaagtaa actatcgagg
    36301 acaagtaacc tttgccaaaa acagtgcggg ttcgatctct catatcgaat gtgacacgat
    36361 catcatggac gaatggtcga aatccgatac gatcccattc aatgagatcc acaacagcca
    36421 agtctcttta gaacacgaag ccaaagtctc aaaaatctca gaagaacaac tctactatct
    36481 gatgagccga ggcctgaccg aatctgaagc aactgaaatg atcgtcatgg gctttgttga
    36541 accattcacc aaagaacttc caatggaata tgcagtcgaa ctcaatcgat tgatcagtta
    36601 tgagatggaa ggcagtgttg ggtaaacctg ttataggagc tagaaccctt gatatataag
    36661 ggttctagct tttttgtttt gcctgtatac tagaaatgac cagattttga ccagctcttt
    36721 tactgatgag ccatcatatc agcaaagtct tgacctgttt tcttaggtgc attatttgta
    36781 atatgtgcat agatgtctaa tgtcacttta ctattttcat gccctaatcg ttgttgaact
    36841 accttgacag attctcctgc ttctaataat aaactagcat gtgtatgtct aaacccatga
    36901 ggtgtaatac gaggcaagtt atattttttc aaaatatatc ttaaccaatc attaacagtg
    36961 tttggaatgt atagagtgtt tttaactgtt gtaaaaacat gttgctcttt agagctagta
    37021 ttgtatccaa gttttaaata ctcttctttt tgctgtaatt tccagttgtt gaggatagaa
    37081 agagtctcag tatctaaaga gataactcgt ctagaagctc ttgttttcgg agattgaatt
    37141 ataactttac caaattcatc tacagcaaga gttttattga catgtagttc tttattaaac
    37201 gtgtctatgt ctttccactg taaagcgagt acttcactct ttctcatacc tgtgtatgcc
    37261 aataatctga agaaggcgta ttgtttcatg ttaccaaaat ctttaaaagc atccagtaaa
    37321 ttattaagct cctcttttga gtaaaattta cgatttttct catactcttt ttttcgtgga
    37381 agaagtgttt tttccatagg attattgtca atatattgca ttgcaacccc gtagcgtaaa
    37441 atttgtgcag tctgttttct catataatcg taactttcat atttactgtg ccattcatct
    37501 accacttttt gacaatatgg gacagagata ttcgatagtt taagcttacc aaatgctgga
    37561 agtatttgat tatttgcgta tcttactgag attgcgattg ttgaagcttt aacactggta
    37621 cgctgatgag ttatccattc attatacagg tcttcaaatg tcatatcagg tgctttcttt
    37681 ttaacaccgt ataatatttg atgtctgtac tcgtcataat ctttttgagc ttcttgtttg
    37741 ctactaaaac ctcttctcgt agtatttatt ttttcaccag ttcgtggatc ggtggcaatg
    37801 tgtcctttat acatccacgc cacttgaccg tttttctttt tgtactttga tatttttgcc
    37861 atgaaatcat ctcctcttag tttacaagga gtacttacca ctagatggta tcacctccct
    37921 tcattttttt cttagtgtat tttttcccaa ttgatgaaat cgtttgttct agcttgttta
    37981 tattatcagt aatttctctc acagcttctt cgggataaga catgtctttc aattgaatag
    38041 gtagccattc atctaccatt ctttctagta gttcttctat actgctagtg aggaaaagaa
    38101 tgttattgtc aaattcttct acaatagcat tgatgaataa ttcttctatt tttaagatat
    38161 tgaaaagact aattttttca tttttaactt tattacaagt cttgttagca ataaattctt
    38221 gcatctctgg ttctaatttg gaatatctat ccagccatgt atctaaatta gagtgtactg
    38281 gtgggaactg ttgacgaaga tgctggaaca ggtttttatc tggaaatgtt ataccatcta
    38341 gtacaatgct atctctataa agaataattc ccaaaatata ttcttctaca ttgccacaaa
    38401 ctaactcctc tatactcata tttcccaaat cagcaattcg ggataatcgc tctggtattg
    38461 gtaaataggt tcccttctcc cattcgtaga tagtcttttt gtcaattaat tgaccgttgt
    38521 taatcataag accaaatgct ggttgtgtta attttttact ctttcttata gcttttattt
    38581 ttgttccgag tatctttttt cgttctaaat aatttattgg tggacgtttt atcatattga
    38641 ccacacctcc taaaattagt atacctcaaa aaaggggtgt tgtaaataaa ttttacaaaa
    38701 ctattgacac ccctttttta tcgaggtatt atgtgtatgt cgatgaactg gtaatcattt
    38761 tttgtttgat taatcggtta ataacaaaat acttttattt tgagcaaaaa atacgaagat
    38821 aattcttaga aaatttgttc aagcaatcac gaaaggaggg ggaaatatgt ctctacaaat
    38881 cattgatagc attgtttcta tacaagttga acaacaagtg aaagagataa ttacagtgct
    38941 aaagcctatg ctagaagaca ttatttctgg agtcactcta gagtatatgg actatcagca
    39001 ggcaagtgaa tatctaggtg tatcagtagg aacgattaga aaatatgttt ctcaatacgg
    39061 gttacctgtt ataaaaattg atacggtcat tagatttaaa aaatctgata ttgatag
//
LOCUS       DAGCYL010000007        35385 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.007, whole genome shotgun sequence.
ACCESSION   DAGCYL010000007 DAGCYL010000000
VERSION     DAGCYL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.007"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(98..1117)
                     /locus_tag="MB216_000258"
     CDS             complement(98..1117)
                     /locus_tag="MB216_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4525121.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1139..2146)
                     /locus_tag="MB216_000259"
     CDS             complement(1139..2146)
                     /locus_tag="MB216_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBT4525122.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2139..2804)
                     /gene="pgmB"
                     /locus_tag="MB216_000260"
     CDS             complement(2139..2804)
                     /gene="pgmB"
                     /locus_tag="MB216_000260"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBT4525123.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2797..5091)
                     /locus_tag="MB216_000261"
     CDS             complement(2797..5091)
                     /locus_tag="MB216_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBT4525124.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5351..7516
                     /locus_tag="MB216_000262"
     CDS             5351..7516
                     /locus_tag="MB216_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4525125.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7820..8653
                     /locus_tag="MB216_000263"
     CDS             7820..8653
                     /locus_tag="MB216_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBT4525126.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8755..10455)
                     /locus_tag="MB216_000264"
     CDS             complement(8755..10455)
                     /locus_tag="MB216_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525127.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10962..12122
                     /locus_tag="MB216_000265"
     CDS             10962..12122
                     /locus_tag="MB216_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4525128.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12138..12581
                     /locus_tag="MB216_000266"
     CDS             12138..12581
                     /locus_tag="MB216_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4525129.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13064..13594)
                     /locus_tag="MB216_000267"
     CDS             complement(13064..13594)
                     /locus_tag="MB216_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBT4525130.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13699..14250
                     /locus_tag="MB216_000268"
     CDS             13699..14250
                     /locus_tag="MB216_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4525131.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14403..14876)
                     /locus_tag="MB216_000269"
     CDS             complement(14403..14876)
                     /locus_tag="MB216_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4525132.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15014..16486
                     /locus_tag="MB216_000270"
     CDS             15014..16486
                     /locus_tag="MB216_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBT4525133.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16483..17193
                     /locus_tag="MB216_000271"
     CDS             16483..17193
                     /locus_tag="MB216_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBT4525134.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            17545..17829
                     /locus_tag="MB216_000272"
     CDS             17545..17829
                     /locus_tag="MB216_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBT4525135.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18110..18529)
                     /locus_tag="MB216_000273"
     CDS             complement(18110..18529)
                     /locus_tag="MB216_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBT4525136.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(18531..19115)
                     /locus_tag="MB216_000274"
     CDS             complement(18531..19115)
                     /locus_tag="MB216_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBT4525137.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(19162..20637)
                     /locus_tag="MB216_000275"
     CDS             complement(19162..20637)
                     /locus_tag="MB216_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBT4525138.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            20818..21129
                     /locus_tag="MB216_000276"
     CDS             20818..21129
                     /locus_tag="MB216_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525139.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(21244..23580)
                     /locus_tag="MB216_000277"
     CDS             complement(21244..23580)
                     /locus_tag="MB216_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4525140.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            23722..24390
                     /locus_tag="MB216_000278"
     CDS             23722..24390
                     /locus_tag="MB216_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4525141.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            24393..25436
                     /locus_tag="MB216_000279"
     CDS             24393..25436
                     /locus_tag="MB216_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4525142.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(25673..26554)
                     /locus_tag="MB216_000280"
     CDS             complement(25673..26554)
                     /locus_tag="MB216_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4525143.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            26713..27441
                     /locus_tag="MB216_000281"
     CDS             26713..27441
                     /locus_tag="MB216_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBT4525144.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            27597..27914
                     /locus_tag="MB216_000282"
     CDS             27597..27914
                     /locus_tag="MB216_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4525145.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            27907..28515
                     /locus_tag="MB216_000283"
     CDS             27907..28515
                     /locus_tag="MB216_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBT4525146.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            28570..29361
                     /locus_tag="MB216_000284"
     CDS             28570..29361
                     /locus_tag="MB216_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBT4525147.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            29499..30011
                     /locus_tag="MB216_000285"
     CDS             29499..30011
                     /locus_tag="MB216_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBT4525148.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            30037..30909
                     /locus_tag="MB216_000286"
     CDS             30037..30909
                     /locus_tag="MB216_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBT4525149.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            31085..31447
                     /locus_tag="MB216_000287"
     CDS             31085..31447
                     /locus_tag="MB216_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBT4525150.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            31456..32208
                     /locus_tag="MB216_000288"
     CDS             31456..32208
                     /locus_tag="MB216_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4525151.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVKPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            32222..34024
                     /locus_tag="MB216_000289"
     CDS             32222..34024
                     /locus_tag="MB216_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4525152.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            34301..35341
                     /locus_tag="MB216_000290"
     CDS             34301..35341
                     /locus_tag="MB216_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBT4525153.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT        11053 a         6255 c         7288 g        10789 t
ORIGIN      
        1 ttttttacag ataaacaatg tgttagaagt agtatcttct tttacataaa aagctgacca
       61 taggataaat ggtcagcgta tttagtgagt cagctattta gtatgggttt tctaaaggaa
      121 cgactgtctc tcttttgatc aagcgatgag ggatgaccaa acgtacgcca ctgctcaatt
      181 cttcatggat caattcgcgc agcttttgca tagcgacacg tcctaattcg gagatatcga
      241 tatcgatact tgtaaggtaa gggtgtgtta atgttgagaa gatcgagttg ttgaagctaa
      301 tgacggagag tgtctctgga atgacatagc catacatttg cgccaactgc atcgtgcgaa
      361 gggcgaagat atcgtcaatg accaccaatg ctgtggcttc gttttctttc aacgttttct
      421 caaaggacag ataatcctcc ggatgctcta gtactacact ggataacgca ggaagaccag
      481 ccatcatcat tgctttttga taaccgaaat atcgttcgaa atacaaactt tcatgagtcg
      541 tgtttgtcac aaacaggata ttcttatgtc cattctggat caaaaattct gttgcttgtt
      601 tacctagaag ctggttatca ttatctacat aaacgatttc gttctcatga tgataaggct
      661 gtccgatcaa agtgaaaggg atgtggtttt ggtataaata atcaatgaca gtatcttgac
      721 tatctgaata cactagaata aaaccatcta cttgtttttg taaatgcata cgctggacgt
      781 tctctaacag caattcaaaa tttttagcag tagcgattgc tgtagtcata tcataattcc
      841 gcgcttcttc gttgattgct tccatgatct caagataaaa tgggttaccg atccgctctt
      901 ttgaatcaag agggggtaag attacgccga tggcactgga aatgcgtttt cctagattgc
      961 gggcagtcat gttaggaaca taacctagtt catccataat ttttcggact ttcttctttg
     1021 ttgtctcgga aatactagga tgatcgttga tgacacgaga gacggtcgag gtggcaacac
     1081 cggctttttt cgccacatct tttacggtaa tagccatgat aaaaacacct ttctcttgtt
     1141 attgtgcaga gaaccgataa atcgtctgac tgatccgttc ttgaccagga tggaggttga
     1201 tcgatccaaa tttggggaaa tgaacgatat cagggatttc ttgcggttcg attgctaatc
     1261 cgtagtttga atgcatctgt tttccattga cagagaaatc agcttcgaat ccagtcgtag
     1321 aaaataatac catactttta ttagaagttg ctatagtcat ttggcgacca gaatgcttct
     1381 cgaataattg taaggaagtt ttggaaagcc taggatgatg caaaagaaat acatcatcta
     1441 gtccatctgg ataacatgca aggatctctt gcagtgttgg ggattttcta aagtcgattg
     1501 gcagtttttc tttttttgtc caatttccag taggcagttt ttcttgatct aattcaagca
     1561 caccatctaa aaagaccgtt aactgatgtt catagatgtc tcttgttcca tttcctgaaa
     1621 ggttgaagta ggcatggttg gtcggattgc agatggtttc aacaaggcta ctagcagagg
     1681 ttgtcatctc taattcgttt tttgttaaac ggtaagtgat cgtagcagtg attggtcctg
     1741 gataggtaga aaattcatct tttaagtaaa agaccacacc tattgatgat ggagttttga
     1801 aaacttctac ttcccaaaat tggaaagacc agcctttcgt gccaccgtgg atatggtgtg
     1861 atccgcagtt tttctccaat ctatgctcac cccaagaagc atcccggatc cttccggcta
     1921 caggtccgac agttgctcca aaaaagcttt tgtctgttag aacatcttcg aaacgatcgt
     1981 aagacaataa aacattttct gattttcctt ctttgtctgg tgcgaagatt tggtgcaggc
     2041 gggcaccgta attgagaaga acgacttcta agtagtcgtt cttcaatgtg attttcttta
     2101 tttcagtctc tggaactggt acgacggaga tctctggatt atccatggtc atgccacttt
     2161 tctttcaagt aatcgatcgt aagaaaagtc gtatcaggga caagcgggat ctcctctcca
     2221 aggtcttcag cacgtcccac gccaattggt tgagcgccac ttgagaggat ggcttggata
     2281 ccggcttgcg cgtcttcgat cccgatacat tcttctgctt ttagtccgac agcttttgct
     2341 gcaaggagga agatatctgg cgcaggtttt ccgtttgttg cttctgccgg gtcggcaata
     2401 gcatcaaagt aaggtgtaag acccattttt tcaagtagga atggcccgtt tttacttgca
     2461 gaagcaagag caatcttgat tttttcttct tttaaagaat ccagtaaagg cacgaccccg
     2521 gggaagacat cttttggttc gatcgtctgg atcatctcta aataataatc gttttttctt
     2581 ttcgctaatt ctgcaaattc ttcctttgaa aaagagtgtt cttttccgcc ataagctaaa
     2641 agcaaagaga gtgagtcttc tcggctgacg ccttttagct gttcgttgaa tgtgtggtcg
     2701 atcgtaatgc cgatttcctg accaagcttt cgccaagcac gataatggaa ttcagcagta
     2761 tcggtaatga caccgtctaa atcaaacaat acccctttaa acatttgctt cctcctttct
     2821 tagcgttatt tctagacgat ccgctaaaga ataagactgt tcatagacgt aaatttctag
     2881 aggagaacct tctaataact cgaaagtaac ttgttcatct gtgactgaga tgaacaataa
     2941 acgtccgcga taattgatat ggaaagaata agctttccaa gtttttggca gaaatggtgc
     3001 gaatctcaac tgatcatggg cagttttcat ttgagcaaat ccttggacaa tcgacaacca
     3061 gctaccagtc atggaagtga tgtgcaagcc gtcttctgtg tcgttattgt aattatccaa
     3121 atccaagcga gctgttcgct ggtacatttc tacagctttg ctttccattc ctaattctgc
     3181 tgctaaaatc gaatggatac ttggcgaaag ggaagactca tggacagtca ttggttcata
     3241 gaaatcaaag tttttttgct tttcttcttt tgtaaaagta tcattgaaga aataaatccc
     3301 ctgtagtaca tctgcctgct tgatgaagca agatctaagg attttgtccc aagaccaatg
     3361 ctgattcaga ggaagatcgc ttgtgggaat atcggataca ggcatgagat ctttatctaa
     3421 gaatgtgtca tgctggacaa agattcctaa ttcttcgtca actgggaagt acatgttctc
     3481 tgcgatatct ttccatttgg ctagttcttc ttcgctgata gtgatagcag cttctttttg
     3541 ataagttaaa taattttctc gcgtgtaacg caagacccaa gctgcgatcg tatttgtata
     3601 ccaattgtta ttgatattgt tctcatattc gtttgggccg gtcacaccgt ggatcatgta
     3661 tttatttgct cgttttgaaa aatggacacg gtctgcccag aagcgtgcga tttctgctaa
     3721 aacttccagt ccttcatttt tcaaatattc cgtatcgccg gtgtaattcg tataattgta
     3781 gattgcatag gcaatcgcgc catttcggtg aatctcctcg aatgtgattt cccactcgtt
     3841 atggcattcg acaccagtga aggtgaccat aggataaagc gcgcctttta atccttgttg
     3901 ctgagcgttg tggattgctt gcggcagttg attatgccga tatttcagca gattcttagt
     3961 tacttcaggt ttggctaaag caagataaag agggacagca tacgcttctg tatcccagta
     4021 agtggctccg ccgtattttt ctcctgtgaa tcctttgggg ccgatattca atcgttcatc
     4081 ttcgccgtag taagtagaga atagttggaa caaattgaat cgaatacctt gttgtgcttc
     4141 ttcgtctcct tcgatgacca catcggcaag cttccatcgt tctgcccaag ccgctgtttg
     4201 ctcttctttt gcttggatat aattgtttgt ctggttcgtt aattctgaga cagctttttc
     4261 tgcttgctgc gcttctggaa tatctctact agtaagaacg ataatttctt tttccactga
     4321 ccatgtctca ccttgagata attctgcaga caagcaacca tgtaatgcta aagtatcttc
     4381 gtgatagtca gggatgactg attttccatt aacgaaatgg cgcattgctg cagtaacagt
     4441 aaattgttca atcccaaagt tgtttggaat agttttagtc gtcaaatagc cgatttcttc
     4501 tatagttcca cgatcaatct cattccaaaa atgttcatca tagttgctgt cttcgttttg
     4561 aacatttcca tccaattttg atacgagttt gactgttccg ctaccttcaa gcatttctat
     4621 agttaaatag atataagctg attctttttt gacaatgctc agaaaacgtt cgaaggaaat
     4681 gcgtacttta ttagtagaag tagtgattgt gaatgtacga gataatacac catttttcat
     4741 atctaattcc cagtaaaagt cttctggttc caaagaagcc aagtcgatca gctgatcgtt
     4801 gatcgaaata tctatcgcga taaagttcaa ggcgttgatc acttttccaa agtattctgg
     4861 ataaccattt ttccaccagc cgacacgtgt cttatcggga taccagacac ccgccaaata
     4921 agtaccttga tggtggtccc ctgagtaagt ttcttcaaag ttcccgcgca tccccatata
     4981 accgtttcca atacttgtta aagattcttg cagtcgaaga ttttttttat ctaattgtgt
     5041 cgtgctgatt ttccatggat caatagcaaa tagtcgtttg atctgtttca tttttctcct
     5101 cctaatgagc tgttttatac gttcataata aacgcaaccg attgcacaag tcaatagaca
     5161 aaagaaaata tttcatagaa aaaagcttaa aaaaataaat ttttaggaga aaattataga
     5221 agggaacaca cgtattatag gttttataga atggtataga cagaaaaata aaaaataaag
     5281 catttacaaa aaagaaaacg cacgctataa tacgtttaac gaaaccgatt gcgtaaagga
     5341 gctaaacgga atgaaaaaga tatttagttt tgaattttgg caaaaatttg gaaaggcttt
     5401 aatggttgtg gtagcagtta tgccggcagc tgggttgatg atcagtatag ggaaatcaat
     5461 tcccttgatc aatcctgatt ggacgccgct tgtcacaacg ggcggagtaa ttgaaaatat
     5521 tggttgggca attattggta atcttcatat tttatttgct ttagcaatag gaggaagttg
     5581 ggcaaaagaa agagcaggcg gagcatttgc agctgggatc tcatttattt taatcaatcg
     5641 gatcactggg gctatctttg gtgtcacaag tgacatgctt gcgaatgaag atgcatttac
     5701 ccacactctg ttcggtacga agatcatggt taaaggattt tttacaagtg tattggaagc
     5761 accagctttg aatatgggag tattcgtagg gatcatcgct ggatttgtcg gggcaatggc
     5821 ttataataaa tactataatt atagaaaatt gccggatgct ttgtcatttt tcaatggaaa
     5881 gcgctttgtt ccgtttgttg ttatcttatg gtcaacaatc gtggcattga ttcttgctgt
     5941 ggtgtggcca aacgtacagg ctggaatcaa taattttggt ttatggattg cagaatcaca
     6001 agaaagcgca ccgattctag caccgttttt atttggaaca ttagaacgtt tactattgcc
     6061 atttggattg catcatatgc tgacgattcc tatcaactat acacagcttg gcggatcgta
     6121 tgagatttta tcaggcgcac aggcggggac acaagtattt gggcaagatc cgttatggtt
     6181 ggcttgggcc actgatttag tgaatcttaa aggtgcaggg gatatgtcgc aatatgagtt
     6241 tgtgttgcat aactggacgc cggctcgttt caaagtagga caaatgattg gttcttcagg
     6301 tatcttgatg ggaatggctt ttgcgatgta tcgtaatgtc gatcctgata agaaagcaag
     6361 atataaatca atgtattttt cagcagcgtt ggctgtcttt ctaacaggtg taacagaacc
     6421 attggagttc atgtttatgt ttgctgctgt gccattatat gtcatttatt cagtgatcca
     6481 aggggctgca tttgcgatgg cagatatctt gccattgcgg gtccattcat ttgggaatat
     6541 cgaattgctg actcggacac cgttagctat caaagcgggt ctaggtggag atcttatcaa
     6601 cttcgtgttg tgtgtgatcg cttttggtgt tgttacgtat ttcttagcaa atttcttgat
     6661 caaaaaattc aactttgcta caccgggacg taacggaaat tacgacaatg acagtgaaga
     6721 aaccgttgta tctaacagcg gaacaggaac agcagatcag caagtggtcc agatcattca
     6781 tttgctggga ggaaaagaaa atatcaaaga tgtcgatgct tgtatgactc ggttacgcgt
     6841 aagtgtaaat gatcgagaaa aagtagggac tgaagaagcc tggaaacgag caggggcgat
     6901 gggattgatc gtcaaagaca atggagtcca agcagtttat ggaccaaaag cagatgtctt
     6961 gaaatcagat atcgaagatc tgctgcaatc aggagcagag attccagagc cagaaatggt
     7021 ttctacagga aatggacaag caggtagtaa gcaatactta ggtatagaac aagaactagt
     7081 ttccgcagct tctggagaag tgattccttt atctgaagta aaagatccgg tattctcaca
     7141 aaaaatgatg ggtgacggct ttgctgttat tccaacatca agagaagttg ttgcgccgat
     7201 cgctggaaaa gtgacaagta ttttccctag caaacatgca attgggctgg aaacgaaaga
     7261 tggtatcgaa gttttgatcc atatgggaat cgatacagtc caaatgaatc agccagcttt
     7321 tgagattgct gtcaaagaag gccaagaagt aggaacagga acgaaattag ctgagatgga
     7381 tcttgatgtg atccaaaacg aagaaaaaga tccaacgatc atgatcgtat ttacaaacga
     7441 taaagtagaa gaagtagtga tcaagcaatt aggtacagtg acagctggta aagtgatcgg
     7501 ctccattaaa atgtaaggag aggttgtgaa aaagctgtcc tgcatcaagt cataagaaca
     7561 gccaaacaaa aatagctcct cctatttttc gtttggttgg gcttattacc gagaagcagc
     7621 agtttgaata ctgtttagtg gagaggtcgt gaaaaagctg ccctgcacca agtgataaaa
     7681 acagccaaac aaaaaatagc tcctggtcca ttgagcttat tactgaggag ttagcaggag
     7741 aacactattt gctggagagg ttgtgacaaa aattttgtca caaccctttt gtttcgaaat
     7801 gaagacaagg agaaaaccaa tgaaattcat gacactcaac acgcatagct ggcttgagcc
     7861 agaaccagag aaaaaattgc aagaattagc agataagatc ctgttagaag attatgaagt
     7921 gattgcttta caagaaatca accaattact ggaatctgaa gaagtagaac ctgcgaagct
     7981 aatgaagttc tgtccggtaa agaatcaagt acctatccgt aaagataatt ttgcctaccg
     8041 tttagttcag cttttaaagg aacacggaaa agagtatttt tggagttggg aaatgagcca
     8101 catcggttat gataaatatg aagaaggaaa tgcactgctt acgaaaaaaa cattggaaag
     8161 tcaggtcctt accgtctccc aatttcaaaa gaaagaagat tatcagacac gcaagatctt
     8221 gattgggaaa acaaagatag atgatcagga catttttgtc tcgagctgcc acttttcttg
     8281 gtggacagat aaaaaaagtg gcttttattt tgagtggaaa aacttggaaa actacttttt
     8341 ggaaactcgc gtaccacttt ttttcctagg tgatttcaat aatccagtag actctcaagg
     8401 ttattatact gtaagagaaa gttgccttct tcttcaagat tcgtatgttg tcgcgaatga
     8461 aaaaggaaaa gcagctactg tggaaaagaa aatagatggt tgggaacaaa atacagaaaa
     8521 attgcgtata gattttattt tcgtccctga aggaatgcag gttaaaaaat atcaacggat
     8581 ttttgatggg atcgatagtc caatcatcag tgatcactac ggggtggaaa tcgaattaga
     8641 tgtgaacgaa taaaaaatat aacatataac ataaaaaaac tgattaaaat aaaactctcc
     8701 aaacgattat accgtttgga gagttttttg atcggatgta tttatccctt ttgcctaagc
     8761 acgtaaaagc ttgtatctat gataaataaa taaacatagc aaggtaaacc agccaatcaa
     8821 agtaagagaa agaatgaaat agaaatagat tggcgtttga taatgaagcg tgagctcgtt
     8881 gattcctttt tgctgcttca cgattggggt cccgatatct gttacttcat aatcagtcaa
     8941 tgtttttcca ttttgctgta gcacagtccg atcgtagacg ataacaggga tatgaacagt
     9001 tttgtcccca gtgtttttcc acttgattgt caaaagaccg cctggatgtg tttttataaa
     9061 agttgtttcg ttttctagta tttcttcgtt ataccggtcg tatttattgt cttttgtctc
     9121 cttatagatt ggcagatagt caggtgtggt tttttggaaa acgaacaagc tggtagacat
     9181 atctttgctg aagaatgttt tccttgcttc ttccggcgta tcgaatagat aagtatgtcg
     9241 actgacaaat gtttcatttt cccacttttc cagatgctgg tataagttct ggatcgtctg
     9301 tgctgaaaaa ataagaatgc caaatatact taaaagacgg aaccactttt gttcagacca
     9361 ctggttcagt gaaagggcaa ggtaaagtag aagcaatagc gtaaatggca cgaaaaatcg
     9421 aaatggaaac tggatcaaac taataaaagg cacatttttt tcgatcaatg tcgtccatgg
     9481 aatcaagcta gtagaaagaa aaaagaaaac aagacttgtg tacaacacga ttttttggaa
     9541 taaggtgttc ttttgttgaa agaaaataaa aagcaaaaaa cctgataata tcaatatagg
     9601 aaaggcctta gggtaatgga gccaataact gccatccaga tctatcgcct tttgactcat
     9661 ttctctgtta acaaagggag gaagaatctt attcccactg taaacaatag ctaatcctgc
     9721 ccaaatattt gctgtcagaa caaaataaag caagaccgac ttaatcagtt gaagcagtgc
     9781 ttttcctttt tcagtctggt taaaaaagta ataaagataa aaagggatat acatcaaagc
     9841 taacatgatc gaagacagta aatggatctg tgtcataagt cccactgaga cagcgacttt
     9901 gataacaggg aactttttat gaaggacgaa atcagcaata gggatcatac ataaaggata
     9961 aaaacaagtc ccccagctag tgaatccttg gttgatagac cagtattgaa tagaatacgt
    10021 actcatgaat aaaacggcta ctggtacact tacccaattt cgaacattga catatctcag
    10081 taaaatcagc atagaaaatc ctgaaataag ataaagaatg aaatttgaaa gaatctgata
    10141 atgaaaccag ctacctgcaa gcaacacgat caatccttgt aaataagcga aaaatgggcc
    10201 gtaaacggca ttcacgatcc tgccactttg ctggaatcca taaagggaag gaaaataatg
    10261 gaaatcccca tatttaattt gttgcgcggc atcatagaac cgattgtaat ggaatggggc
    10321 atcagcaccc aaaattagtt tatgtgtgaa taaatgaggc atgagaaatg ccaaagcaat
    10381 gaaaaaaatt gctaaaaatg ggccatattt tttcaattgt cgaactcctt tgcaaacttt
    10441 agataaattc atcatatatc ctatgcgaga ggtaggcaag aaattagaaa actcatgaaa
    10501 atatttatga attttacaag aaaaacataa gtgaaagttc taaggagaga ggatgcgaca
    10561 taagttttgt tgcatctcct tgttttgaaa cagaattttt cttaatactc gaaacaaagt
    10621 agctttttca tgacctctcg gtaaacggtg ttcaatcagc tgcttctcgg taataagctc
    10681 actagcctag aaaatatgag aagctatttt ctgactagcg ctcttattac tcaaagcata
    10741 acgcagtttt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    10801 gcccaaccca acaaaaaata tgagtagcta tttttttggg ctgtacttat tacttgatgc
    10861 aggacagctt tttcacaacc tcatcggctt tcattttcta tttttttcct gaacccatat
    10921 aataaaagaa agaacaaaaa aacttgcaag ggaggacaca tgtgaagcag agtaagcaaa
    10981 aacagtctcg tcgacttttg tttcgtttgt ttttattaat atgtatcgtc gtagggttac
    11041 tgatcgcttt atatccattt tatgtggatt ctttaaatag ttttattgat caaaaacgta
    11101 tcgaacaagt acaaaaacgg accgcagctg aaattgaagt gcagcgaaag aaaatggagg
    11161 agaaaaacca acgattaact gaccaaggat tcaatcctgg tactgatcct ttcaatgaaa
    11221 agaacagaaa tgaaagtaca acatccagtc aattagagga atggctgatc ggaagtatca
    11281 atatacctaa gatccaatta aatatttcac tttatgatcg gctcaatggt aggatccttg
    11341 aaaatggagc aggcgtcttg caaggaacct ctttcccgct tggtggaaat tcgacgcatt
    11401 cagtgatctc tgctcacagc ggtttaccta atcgacggtt atttaccgaa ttagatcgtt
    11461 tagaaaacgg agacacgttt attttgactg ttttggggga gaaattggct tatcaagtta
    11521 aaaatataca agtggtactg ccagatgaca cgtctgttct aacgatttca gaaggagaag
    11581 acttagttac cttgcttaca tgtacgccgt atatgatcaa cacgcatcgt ttgctagtga
    11641 caggacatcg gattccttat aacgaatcgg taaaaaaaga agaaggaaaa ggaaatcagg
    11701 aaagaaccat gcggcaatgg tttattcttg caggaacgat aatagctgta gtcatactgt
    11761 tagtgtttat cggacgacta atctatcagt atagattaag taaaaaagtg cttgattttt
    11821 catttattat tagtgattcg tcagggaaac cggtaaatgg tagttcattc attttgaaac
    11881 ataagaaaaa aacacttact agaaatggtg tgccgttttc agtacaaagc gaccatcatg
    11941 ggaaagtcaa gctggatcaa ttgcctggtg gaacttatcg aatcgtttca gctgatgatc
    12001 ctaaagttgc tgtttctttt ggtattagaa aagtaaaaca agagaagata tacttttttg
    12061 aggggcgaaa acttgtaaaa gagcttcaaa aaaatggttt ttattttaaa atcaatgaat
    12121 aagtaaggag agaaaaaatg atccgcacaa caacaaaatt gtctgccaaa gaactggaat
    12181 ctgttctatc gatttggctg catgctaact tggattctca tgaattcatt ccatcaactt
    12241 attgggagaa tcaagtagaa tctgttagga aagctttacc agatgcagtg atttacttag
    12301 cagaaataga tcaagaaatt attggttttg ctggggtgat tgatcagttt atcgcaggaa
    12361 tctttgtgaa aagggaatac cggcaacaaa gaatcggtac gcaactaatg aaaaaaataa
    12421 aagaaaccaa taaaacactg actttgacag tctatcaaaa aaatcaatca gctattcatt
    12481 tctatctttc acaaggtttt acaattcaaa cacagcaaaa agatgaagca gtaaatgagg
    12541 aggaacttgt gatgaaatgg aatcaaaacg ctgatttttg atgaacagaa taacgaaata
    12601 atgctcccag cctaattttt tgctgggagc attatttcat tttgaagtca ctaatttaac
    12661 tgtgtgaata tgcagttgtt acaatctttg ataaacggtg ttcaatcagc tgaccctcgg
    12721 tattagctca aaaaacggca aaatatgagg aactattttt ccatttgttt ctctaatact
    12781 caggtcaaaa cgctgatttc acaacctctt gataaacggt gttcaaacgg ctgcttctcg
    12841 gtaataagcc cactagccta gaaaatatga aaagctattt tctaactagc gttcttatta
    12901 ctcaaagcat aacgctgatt tcacaacctc ttgataaacg gtgttcaatc agctgaccct
    12961 cggtattagc tcaaaaaacg gcaaaacatg aggaactatt tttccgtttc tttctctaat
    13021 actcaggtca gaacgctgat ttcacaacct ctctttcaca accttatttt actcgtttat
    13081 aattttcatc gaagtatttt ccgctacgga tatcatctgg cattccatcg tatggttctt
    13141 cgctaataac atcttcattg tctatacctg catcacccat ataagtgatc gtagcaaatt
    13201 caggaacaag taattttgga taagtttcat atttttcacg agctgcttcc atttctgcga
    13261 tatgttcgtt ttggaaagta acagtgattt ctggatgttc ttcaacccat ttagggaaaa
    13321 agatcattcc gtgaagtttc ttttcatttg gcataaagtc caatgccacg tctgtccctg
    13381 ttggctctgt ccaagtgatt ttgaatacgc cgtccgtcaa tttaacgata tcagcttctt
    13441 ggtcttttac ccaacggcct ttgaccattc cgccatggat acggtaatca acagtatggt
    13501 catttttggc ataccattca tattcccaac cattgtcata cgtatagata aagtgagttc
    13561 caataaaatc ttccaattct ttaaattctt tcatgtttaa gcatctcctt ataaattaaa
    13621 acatgtaata aactacatgt tttcgattac tcatttatga tagaatggat ttaaagattt
    13681 gtcaaggagg gaaaactgat ggcaagaaaa aatacacttc gatatatttt gttagggtta
    13741 ttaagtaaga agaagatgac aggatatgaa ttgaatcaat cgttcaaaaa tgaaatagga
    13801 gaattttggc aagcaaaaca tagtcagatt tatcctgaac ttgcaaaaat ggaagagcaa
    13861 ggaatcattc agcatcaagt agagatcaca ggtgaaaaat tggaaaagaa agtttatgaa
    13921 ttaacagaag aagggaagga acaattggca gagtggattc atacacccac caatgaacta
    13981 ccggttaatc gagacgaatt tgttctgaaa ttgtatttcg tcaaagacgt gaatgatccg
    14041 gcattagtag aaattattca gcagcagcgt gaccttcatg aagaaaaacg acttcatcta
    14101 ttagagcgaa aaaaaacgat ctttcctact gaaaaagaaa aacatgaaaa ttacggacac
    14161 tacctgattt tagcgcatgc gatcaatcgg gaaacagaat atgcaaattg gctaacagat
    14221 gtattagaag aaagtaaccg aaaaaaataa aaaaatatcc gaacagtgat ggagaattcc
    14281 tttctatatg aaagaaacgc agatcaccgt tcggatattc ttatgttcaa acaggccttt
    14341 tttcaagtat ttatgtagca tctacttatt aaaaagcttg gtacttagga gattagtggc
    14401 gtttagagtt cttttaacaa ctgttcgtag tcagcttttt catcgacagg aaggagcttg
    14461 taagttaatt cgtctgtctc gttgtcatga atgatgtaca tcttagcatt ctcttctttg
    14521 aagttaccga tttgtaaaat cgtatagcgt tcgtcgatca attcgatatc tttgattgct
    14581 tttgaaaaaa ctttacggac atggtgcatt ttcttttggc ggatttctgc cttttctgca
    14641 gcttttgaca aagtaaatcc gtcatctaaa tattttttga tcagttctac ttttatgact
    14701 gtaggtaaac gatattttct aggacaattc gcatcagctt cgatcgaacg aatgaatccc
    14761 ttttgttccc aataccttaa ttgacgtgga gacacaccta cgatatcact taattcactg
    14821 atgccaacaa ccaattgatc attttccaac aaacgtttaa gggaagcccc aaccattttt
    14881 gtcactcctt ttgtcctctt gcttccctta attatatccg tattaactta tgtgtcaagt
    14941 aggttgacaa atagcgaatt taggactaaa gtattctagt agtttcagaa aaaattcaga
    15001 aagaaggaga aatatgagta acaaccaacc cgtagatatt tacggaaaac catataatcg
    15061 gacactttta gttgttgtct tattaattgg tacattttgt accgttttga atcagacctt
    15121 attgacgaca gcttttccta cattgatgaa agctttcgat atttcagcgt ctgatgtgca
    15181 atggttaacg actggtttct tgctagtcaa cgggatcatg atcccaataa cagcatggct
    15241 gatcaataaa ttcagttcaa gaaatttata tctttctgcc atgacgatct ttttgatcgg
    15301 aacgattact tgctttacag cacctaattt tagtacatta ctaatcggtc gtttgatcca
    15361 agcttgcggt gtgggagtat cgatgccatt attgcaaaat atcatgctat ctattttccc
    15421 gccagaaaaa agaggatcag caatggggat gtcagggatc gttattggac ttgctcctgc
    15481 tttaggccct acattatctg gttatatcat tgataactac cattggcgtg acttgtttgg
    15541 tatggtactg ccaatcgttg ttcttgtctt ggttttagca ttcttcctaa tgaaaagtgt
    15601 tattccattg tcaaatccta gcattgatat tttatccgct attttatcaa cgattggatt
    15661 cggtagttta ctttacggat tttctagtgt aggagatgac ggctggggaa gcttgaaagt
    15721 aattggtttc ttagttgttg gtgtgatttt catcgccttg ttctcatggc gtcaattaca
    15781 tttggaacat ccattcttgg aacttcgtgt attcaaatca tctacattca cgattgcagc
    15841 tgttttagct ggtgtaacaa atatggcgat ggtaggggca gaaatggtcg taccattgta
    15901 tattcaaaat attcgtggag aatcagcatt ccattctggc ttgatgttgt tgccgggtgc
    15961 attgatcatg ggggctatga tgcctgtgac gggtgcgatt ttcgataaat acggcgcgaa
    16021 acgtttagcc atcacaggga tgttcatttt gacagcggca actgcaccat ttgctttctt
    16081 gacaaaaacg actccagttt tatatatcgt gatcctttat gcgatccgta tgtttgggat
    16141 ctccatggtc atgatgccgg taacgacttc tggtatgaat gcattgccaa ataacttgat
    16201 cagtcatggg acagcggtaa ataatacatt ccgtcaaatc gcaagctcga ttggtacagc
    16261 tattttgatc agtgtgttga cgaatgtgac aaaagcaaat atcccaagca aatctgtatt
    16321 ggataatacg ccattagctt ataaagatca ggcaatcaat gccacactgt caggttacca
    16381 tgccgcgttt atcgtagcga tcatctttgg gattcttggc tttattattg cctttttatt
    16441 gaagaaaaaa gaacctgttc taatgaaagc gggggataat gcatgattat tggagttttg
    16501 attgtcagct tgcttgcttt tgctttaacg aacgtgttcg ctaaaaaaac atggcaaacc
    16561 tttttatctt tgattttcgg actaatcttc gtcgcttcac taagtttgat cgtcgctaat
    16621 ttgtccaatc actttggaat ggaaaaagta acggaaacaa aaacagaaaa aattgtttcc
    16681 agtgctgatt cccaaggagc agatatgctt ttgtataaag ctttagggaa tggaaaagaa
    16741 aaagtgtatc tttaccgaac aaatgaaaaa caagaaaaac caaaagcaac tggtacggat
    16801 aacgaaacaa ataaagtaga aaagacagac ggcgatgctg aaaaagtgac gaaaactact
    16861 tattgggaat acaaaaacga catgtataaa ttctggttca atattgctga taacaatcac
    16921 gaatatgaca gccgagtgaa tacattcaaa atcccagaga catgggtaga attaagtacg
    16981 gaccaagcag ccaagttagc tgaattagtg aagaaacagc aaagtacgat ggaaagtgaa
    17041 gcaaaagctt atgttcaaga cggtatggta aaagcaatga ctgaaaatcc aaaaatgagc
    17101 aaagcagagc aagaacaacg tacgaaagcc ttagctgctg aattccaaca acaagcattc
    17161 gcaaaattag tgaaagaagc aaaaggcgaa taatattttg aacaccatgt aggaggttgt
    17221 gaaaaagctg ctctgcatca agcagtaaga acaatcagat aaaaatagca ctccatattt
    17281 ttcatctgat tgggcttagt gccgtagatg caagcttttg aacaccgttt aaggaagagg
    17341 ttgtgagcct gccgtttaac tctgagtcac aaggagcaat ttcaaaaaac agcttctcat
    17401 gtttgttgac tattgtgact tgtgaccgag gagttggcag gagaacaccg tttattcgaa
    17461 aaaaggaact gcgacaagac ttttgtcaca gttccttctt ttttttgcta tgctatacta
    17521 gatgtaaaaa ataggagaaa taaaatgagg gaaatttatt cttttgaatc aaaaatctat
    17581 gcttccgaag tcggcaaggg cggggcatat attatttttc cgtatgatat aagaaaagaa
    17641 ttcggtgctg gaagagttaa agtccaagca acttttgaca atgtgccgta tgaaggcagt
    17701 atcgtgaata tgggcgtgaa aaatccagat ggttcagtct gctacatcct tggactacga
    17761 aaagatattc gtaaaaacat cggaaaagaa atcggtgata ttgtagcagt tacagtgaag
    17821 caaaaataaa agaggttgtg acaaaagacg tgtcacaacc tcttgataaa cggtgttcaa
    17881 aagccagttt ctcggtagta agtcaaattt atgcaaaaat gtggaaaccc atgtttacaa
    17941 aatttttctt actactcgaa acgaagcggc tttatcacaa cctcttgata aacggcgttc
    18001 aatcagctgt ccctcggtat tagctcaaaa acggcaaaat atgagaagct atttttccat
    18061 ttttttctct catactcagg tcataacgct gatttcacaa cctcttcttt cataatggtt
    18121 tttcaccatc tttataataa actggttctt tgatatggaa atctattttt tccaaccaat
    18181 ctaaaatctt gtaacctaac tcttctgaca tcggaaaaga ggcgttgatt tgtgcattgg
    18241 agatatggtc aacatggata ccggaagtga tcgtacaact tcccggaacg gcagttttga
    18301 tgatcacagc tattttttcg gctaagacat cgtctttatg gaaccgacca tcgtggctag
    18361 ggaaacgtac agtttgcatc tctgtatcac gagaaaatgt agtgactgtt ccgatatgtg
    18421 gtgtatctcc gcctgttaaa gtgatcaata agtctttacc gattacttta acagcagctt
    18481 ggatcgtgaa acctagtttg gttacttcaa ctatcgtttc tttttccata ttaatcaccc
    18541 tgccaacgtt ttgaatgctc atttacaatt tcaaattgat ccaaaagttt cattgtatgg
    18601 tgattaacaa gatcttggat cgtttgaggc tgattataaa aagcaggaat tggtggtatg
    18661 atttgcacac ctagtcgtgc taatttagtc aagttttcca agtgaatggc atttaatggc
    18721 gtttcacgag gaacaatgac taactttcgt tgctccttca ttgttacatc agcagcacgt
    18781 gcaatcaggt tgtcgcctaa accaacagag atacctgcga ctgttttcat acttgccggt
    18841 acgatgatca ttccatcggt caaaaatgat ccgctggcga cagcagcacc taaatctttg
    18901 tttgaataat ggtaatcaaa caggctttcg aattcagtta gtgataagtc ggattccagc
    18961 tttaagttct gttttgccca tggactgatg attccgtgtg tttctatcat cggatattct
    19021 tttagtttct tcactaggtt gatggcataa atggtgcctg aagcgccact tacaccaatc
    19081 acgatttttt tcttccgttg tgccaattgt tccattttcc gcactccttt ttatgatgtc
    19141 aaaacagttg cagatcattc attatttcaa atatttttcc caatcttcta cttcttggaa
    19201 ttttgcacgt acaaaatgtt ctttcatatc gaaaggaact gtaccgtcaa agatagtttt
    19261 tgcacttatc ccacggaaac gaatagagcc aggattatat tctggtaatt ctgatgggtc
    19321 caatggatgg ttcctcattc ctgggatcac gatgatatct ttatctcctt ggaaacgagt
    19381 gttgaaagtc caaactacat cgttcatgtc gaaaatatca acatcttcat cgactaaaat
    19441 gactgtcttc aattctttga aggctgataa agcaagaata gctgcttgac gctgaatacc
    19501 ttcatccgct tcgttttctt tacggatttg catgatggtc atcaatttac caccacctgc
    19561 aggaggattg tagacattta ctacttttcc tggaatagct ttatcgacca aggcaagaat
    19621 acttgcttct gtaggaatac cggccataga tacgtgttct tcactgggac caatcgttgt
    19681 ttgcatgatt ggattgtctt ttcggtgagt aactgctttg actttcacta cttgtacagc
    19741 tggattagca tccccagtat agccaggaaa ttctggcatt gcttttcctg agttcgtgtt
    19801 gatatcttct tgcattgttt catttggcat gatttctgct tcaatgatga attcagcacg
    19861 agcaataccc acttcatcga ctgctatacc atcaacgatt tgaataggtt cgttacgcaa
    19921 agctcctgca caccatagtt cattgtagcc aagtggagtg gtaggtggtt cgaaagtcgt
    19981 gccaatggca attgctggat ctaaaccaat attgatagtg ataggcattg gtttattcaa
    20041 ggcttcgaat tgtttttgga agtgaccaat gtggcgtccg ccaggcatga tgtacatacc
    20101 gatcgtatct ttatcttcta gaaccatacg atggatcgtt acatcgctca ttgttttttc
    20161 tggatctgat cctaagacta cacccattgt gatgtatgga ccagcatctt caggagtatt
    20221 tgtaggtgca ggcaaaatgt tgcgaatatc gaagtctgca tcggtagcta gatgaacgac
    20281 ttcttgtgct ggagcgtctg ctttgtctac tttcacaggt ttgattggat tttctacagc
    20341 gtctttcaag aaacgaccaa gtgttcgata atcatggtgc aaaatttcac ctacacgttt
    20401 acgactagcc attgttccga tgttcacacg tgtgccgggg aaacctttga tattgttgaa
    20461 agtcatagct ggtccttctt gagtcggacg ttttactgta ccgccagcac cgatataacg
    20521 gtaaacacca gataattctg cttcaggatc gatttcaata tctgtttcgt gatattgacc
    20581 tggaagtgtt tttagttctt cgatgacttt tcttaaatca tacggttgat ctgtcatgag
    20641 agattcctcc ttagttgtct ttacctatct atagtaaaag gtgaaaaaga ggaaaacaat
    20701 tcaacttgtt cggtaaagta ttctacaatg gaatggatgc gtttttttga taatagaata
    20761 aactatgata tacttatcac taaaagaaag cgcatactta gtgttggagg aaggaaagtg
    20821 aaaaaaatgg aggagtcatt tcctaaagca gtaaaagtgg aaaatatagc gaatattttg
    20881 aaagttacat ttgaaaacgg agaagtaaaa tacgtgaaat ctcattggac agaagagata
    20941 accgatgcct tgcaatttgg aaaaaaagga agaggaaaaa gaaaaaatct tctcgcactt
    21001 tctcgtaata tgtggattgg gactgaagta acgattgaag cagatggaac agtattcata
    21061 aatggaaaag acaggtatac gccagaagag ttgtggtaca aaggaaagaa aagtatacca
    21121 gaattataaa aaataattaa aaaaaacagc gcgaaacaag gaacatatta cttcctcgtt
    21181 tcttgctgtt ttctactatt tcagcgctct tatgactcaa atctaaatgt ggttgtcaca
    21241 accttattct gcacgcaaag cgatagcggc gtcttttttc gctgccattc gtgcaggaag
    21301 atgaccgcca agcatcgtca atatagtaga gacgatcact agaataatcg catggacagg
    21361 atttagttga gcaacatttt ttagatccgt catgttgtat aagatgctat tgatagggaa
    21421 cgtagctagc catgcaataa acacacctaa tatcccagaa gaaataccta aaatacacgt
    21481 ttctgcgtca aatacgcgag tgatgtcttt tttacgtgca cctaaagctt ttagtacacc
    21541 gatttctttt gtccgttcaa tcacagatgt ataagtaatg atactgatca tgatcatact
    21601 tgttactaaa gagatccctg caaaagcaat aaggacataa gtgatagcat ccatcaatcc
    21661 gccagtcagt tcagtcatcg ttccagctaa atcagtataa atgattttat cttcatctga
    21721 tttgccttta ttgtaatcat ctagataatc caagatcttt tctttgtctt caaaattgtt
    21781 cggataaatc atgatacttg aaggcaagct gtctccacct aaataactaa gcaatgtttg
    21841 tttggcattt tcgtcaactt tttcagtagt taagacattt acaccactgt ctctttgagc
    21901 tttgacgatt tctgattctt tgttttcgtt gacgatttga gtcgttaatt gatcgctata
    21961 agcgattcct ggagataaga gattcattgt agaagaagat ttcacgcgta ggatccccga
    22021 gatcttcaat tcatcagaag cattctgata catcgcatca taatctgtat tagggataaa
    22081 attcccagtt ggaagttttg tatagtaagt attgttgttt actaatttga atgttgtacc
    22141 aacgatatca tcaaaatcaa gtttttcgtc ttctttcaca tcaaatccaa gattcttcaa
    22201 tgcgttgata ttagtattat tattgccgtc tacgatcagc acgacatctg ttgctgaggc
    22261 tggataactt ccggcaagta aagaataatt gtcttttaaa aagttttctt tgctagtgtc
    22321 aagctgtgta gggaaacttg acactcccac acctgtcatc gctgacatcg tactagataa
    22381 agaaagagat tccgtatcag gattttgatt cgagaagctg acaggctgta ctttgccatt
    22441 tacatcacgt aaaagattga ttcctgtagt acgggtaaat ccgatattat tacttaagtt
    22501 aggatcgatg tctgttacgt aatcgacgta tgtttgatca atcttattcg tgtgctgtgc
    22561 tttatcttct tcgctgactt ttgcagtaat cgttttgctg tctggataat ccgctttgct
    22621 tgcacccaat ccggcatcgt cacgagtctg gttagtcgtt accttagaaa tagtaatcgg
    22681 aaatttcgcc atcgtttcag cttgcgtgtt atcgatttgt ttttggaatc cagaagaaag
    22741 ggacagaacg atcgcgattc cgatgatccc aatactggaa gcaaaggagg tcaagaaagt
    22801 acgtcctttt tttgttcgga tattgttgaa ggaaagcttt aacgctgtcc aaaaactcat
    22861 ctttgttctg cgtaagttaa attgatcatc ttttggtcgt tcgatgtgag gatgggaatc
    22921 tgttaagatc ttaccatcag aaaattcaat gattcgatct gcgtattgat gggccagttc
    22981 aggattatgt gtgaccatga tcactaattt ttctttcgat aactcttgga tcaattccat
    23041 gatctggata cttgtttctg tatctaacgc tccggttggt tcatcgcaca aaaggatatc
    23101 tgggtcattg gctagtgctc gagcgatggc cactcgctgc atttgtccac cagataactg
    23161 atttggcttt ttatgcatat gatttgtcaa gccgactcgg tgcaatgcat cttctgcccg
    23221 ttttcgtttt tcgtctttag aaacaccact taatgtcatc ccaagttcaa cgttttcaat
    23281 aatgcctaga tgaccaatca agttgtaact ttgaaaaatg aaaccgatag agttgtttcg
    23341 gtaggcatcc cagtcactgt ccttaaagtc tttagtagac tttccgttga ttaccatatc
    23401 accggaatca taattatcta gtccaccaat cacattcagc atagtcgttt taccagaacc
    23461 actaggacct aaaatcgcta cgaattcttt ttgtctgaaa gcgacagaca caccatccaa
    23521 tgctttggtg gtggtttcgc cgactttata atattttttt atatctttta attgaagcat
    23581 aatcaccctt ctcccgtatt gaaatattcg gtatactaat aagtatatca gaacaatgat
    23641 cgtagtatac gaaataaatg ttaactgaag atgaactaca agaaaaaatc tagaaatttt
    23701 tcaaaaaaca gagggagatc aatgaatcgt attttagtag tagaagatga tccgcaatta
    23761 cagttgcttt accgttctgt gttggaacga gcgggctttt cagtcatcac cgcattgaat
    23821 ggagaggatg cgctgaaaaa actagaagcg acacgtatag aattaatcat tacagatatc
    23881 atgatgccga aaatggatgg ctatgacttg ctagactcat tacgtagcac cagatttgac
    23941 acacctgttc taatgattac ggcaaaggcc gattttgagg ataagaaacg cgggttcaaa
    24001 ttaggagcag atgattatat gacaaaaccc atcgatgtca atgagatgat cctacgtgtg
    24061 gaagcattgc ttcgacgttc aaagatcaat tacagcagta gcttgataat cggacaaaca
    24121 aaattagagc tagatgctta tacagtgtta caagatcagc attcgttgat tctgcctcaa
    24181 aaagaatttc tgcttctgta taaattactg tcttatccca acaagatctt cactcgccaa
    24241 cagttgatgg atgatatttg gggattagat acgaatacag aagaacgaac gatcgatgta
    24301 catatcaaac gtttaaggac ccgttttgaa aagacggaag attttcaaat catcacagta
    24361 cggggactag gctataaggc ggttatttaa agatgaaaaa aaagctattt tcacaattat
    24421 ggatctattt tgctagtatt gtcttcattt cgatcttagt gacactgctg tgttttctcg
    24481 gttttatttt cttattgtct cgacattcta tctcgcctgc tgaatcggct ggaaaaccaa
    24541 cgttataccc gttattcgtc tttgcggggt tcagtatggt cgtgggtaca ggaatatcga
    24601 tttttgttgg gcggcgtatc ctgcatccaa tcagtgcgct agggacaaat atgagtctgg
    24661 tagcaacggg agatttttcg attcgaatgg atgaacagca gaaagtagca gaagtacagc
    24721 aactgtataa agatttcaac gttatggtcc aagaactcaa tagtatcgag acactgagga
    24781 atgactttgt gtccagtgta tcccatgaat tcaaaacacc actagcaaca atccaaggat
    24841 atgttcaatt gttacaagcc ccaaatctct ctgacgaaga acgccaaata tttttatatc
    24901 ggattatcga aagtatcacg cagctttcac aattaacaga gaacatctta aaattgaaca
    24961 aactggaaaa ccagcgtatc caactggaaa agaaagagta tcgattagat gaacaaatca
    25021 gagaagtaat cgtttttttt cagcctaaat gggagaaaga acagctagaa ttggatatcg
    25081 aacttgctgc agtaaattac acaggcaatg aagagttttt gtaccaagtg tggctgaata
    25141 tcatggataa cgccataaag tacaatcaaa tcaatggaca gattcatatc aaactatttg
    25201 agacagccac agagattgta ctggaagtga ccgattcagg tgtaggaatg aatgaagaga
    25261 ccagagatcg aatgtttgaa aaattttatc aaggagatac aagtcgtcaa atttctggga
    25321 atgggctagg actttcactg gttaaaaaaa ttctagaact gcatgatgga cgtattgatt
    25381 atagcagtat cgaaggagta ggaacgacag caatgatacg gttaagtaaa caataggaaa
    25441 aagaataaaa cccctaaaaa ttctattgaa ggtttttagg ggtttttagt cgtttttttg
    25501 tgattaggga aatgttctta cggttctttt ggcataaaaa tcgctgcttt cttattgaac
    25561 tacgagaaat gttagaggtg tcattgaaac aaggacatga tgcaagaaag agagcgaaag
    25621 tcatgcgtcg ctctcttttt ttgggtggat agatggacag aaaatgtagg gcttagtttt
    25681 cttcttgatt taaatactcc caaaatgtat ctgaagccaa agaatgctca ccgactttac
    25741 gagtaaatgc aagataactc tctgttgttg gagaaatagg gagaatcaca gtattttctt
    25801 ttaatagatt cttgactgtt ttttccataa caatagcaat ccccatgtca tttgcaatca
    25861 tattgatgat caagtcgatt cttgctcctt tataagagat ttttggttca aatccagctt
    25921 cgcgacacag atctagtaca ggttgataca agttcgtagt ggggccaagg agcaaaaagt
    25981 tctccttttt cagttcgctt agatccaatt tctttttact agctaaagga tgatttttag
    26041 gtagaactgc tacaaaatcg tctgtttcag taggcaacca ctcaatcgaa cttggtgggg
    26101 cctggaaact tctagcaaag tggatcgtgt cactatgttc attggcgtca ttgtaagtta
    26161 attcgacact ttccacttct ttcaactgga ctgtaaattc aggatggcgt ttcaaaaact
    26221 cggtgatttt ggaaaaacct ttataattga tcattgtcgg tatcgttaag atatttaatg
    26281 aaagattgtt gtctttcgta taggtttcaa gcacttcttg catagcatgg tattgttcta
    26341 cgacattttt tacatatggg agcaataagc ttccagcttc ggtcaattcg atttttctat
    26401 gggcgcgttc gaataatttg acgtctaatt ctgtttctaa cgaaagaatc tgtttagaaa
    26461 tatttccttg ggtggtgaat tgtcgatcag cagttttggt gaaattcatt gtttcggcta
    26521 aatcaagaaa aacttcgaat ttatggatat ccatcatatt cctcctactt attccaataa
    26581 agaatacttt aacacaaaaa atgaattggt gtaatcttct ctgtctgtta caatctttct
    26641 tgcagcgaaa gcaataatta cagaaatttt tctgattttt ttcgcttcgt agaaaaagag
    26701 aggagttttt tgatgccaac tggagttttg atcaatgtag gttccgtttt gtttggcgga
    26761 ctaattggcg gattagtagg aaataaatta agtgaacatt ttaaagcaca gctgacgatg
    26821 gtttttggtg tgtgttcgat ggggatggga atctattcca ttgcgccgat gaaaaatatg
    26881 ccggctgtta tttttgcttt agtgatagga acagcgattg gtttgattgt tcatttaggt
    26941 aatgggataa ataagggggc tgcgctgatg caagtaccta tttctaaaat cttccctagt
    27001 gaaaagcttg gtatgaccca cgatgaattt atcagcacat tagtgacggt cattgttcta
    27061 ttttgtgcaa gtggtacggg gatctatgga agtttagatt ctgggatgac aggagacagt
    27121 acgattttga tttctaaatc tgtccttgat tttttcacag cagctatttt tgcgtgtaat
    27181 ttagggtatg tcgtttctgt gatagcaatt ccccaattca ttatttttta tattttgttc
    27241 ttattggcaa aatttattta tccattgact acaccagaca tgatcttaga ttttaaagct
    27301 tgcggcggat tcttgatggt cgctaccggt tttagaatga tcaacgtgaa aatgttccca
    27361 gtagctgaca tgatacctgc aatgatcgtt atcatgccac tgagctggat gtggacaaat
    27421 tggattatgc cactgttata ataaaaatca aagagaagcc attttttgct gactaaaaag
    27481 gatcaaatct tttagtcagt ttttttgtat ttgacaaact gattttgaaa ctatattata
    27541 tgttgtataa tatagattga aataaaatat tacacttgag aatagaaggt gacgtaatgc
    27601 acatccagat tccaactgtt cttcttgatg gtactgtgtt agcagcatta agcaaggaag
    27661 atatgtatgg gtatgcctta acaaaaatcg tacaagaaag tctctctgtc agtgaatcta
    27721 cactatatcc ggttttgaga aggttgaaaa aagaaggata tcttgagacc tatgatgaac
    27781 cttttgaagg cagaaaccgt cgatattact cgttgacacc aatgggaagt cgtcatttag
    27841 aagaaattat tgatgaatgg attgtttttc gagattcttt agaccggtta ttggaggaga
    27901 gcacaaatga atgaatattt gaatgaagtt gtggatcatc ttcaagaaat tcctgaagac
    27961 gatcgttttg atttgatcca gtattatgag gaatattttt tagattctgg aaaaacatta
    28021 gatcagatca ttgatgaata tggtacacca aagcagtttg ccttaaaatt aaaaatcagt
    28081 tacttttcag aagaagaaca agcagaaaac gagttagtga aaaatccaaa aagacaaatt
    28141 cgcctgattt ggttaatcat tatcggttta tttgcttctc ctgtcctcat tccactagca
    28201 ctggggttta tattggttat tgggggacta ttggttgctt tactagcgct catttttagt
    28261 atctatgttt tatgtatcag tatcttaggt gttggcctat tcatgatcat ctcaggaatc
    28321 agtgtgtttg gacaatcgat tctgagtgga ttattcttta tcggtgtcgg tctgttcgcc
    28381 acaggaagcg cagtatttat ttcgccactg ttgctgaaag gaacaaaagg gcttttttat
    28441 gtcatgatga actttgttaa atggatggga cgacgtttta ttacaaaaag agatctgcat
    28501 tcagcgggcg tttgaaggag gacacataaa ttgagattga aacatttttt gattaccggt
    28561 attggtctga tgcttactgg tggcctccta gcaggtgcaa cttatacatt aggcgctcaa
    28621 aagagtctga cttgggataa tggtcctcgg ttgatcgata tggttactga gacaaaaaaa
    28681 atcaaggatg caaacaaaat cgtcatccat gcagaaaatc aaacagtttc ggtcatacgc
    28741 ggattggatt ttgagataaa aacgacttat gataagacag aaaagccaga aataaaggag
    28801 aaaaatggcg tgttgactgt gaatgcagtc aaaaaatcaa caggtgcaat cattagttta
    28861 gaagacacaa gtgcacaaat cactattgtt gtccctgcag atcatgaatt atctgaattg
    28921 acaatcgaag ggaataatag tgcaattgat atcaacgaag tatcaatcaa aaaagtgaat
    28981 actaagacac atgatggctg ggtatcgttc aatgaattta caggaggaac aataactgcg
    29041 aaagatgaat taggctcact tcgactagat gaggccaatg tggataactt gatcgtcaca
    29101 ggtgaaaaca gcaacttcca tctagaagac agtttgatca gcaaagaagg aaaagtcatc
    29161 ctaaacggtg gtaatttatc tttgcatgag atggaaaaca gtggctatac tatccaaacg
    29221 tctggtgaat cttatattga aaaagactac cagccaataa caaatacttt gttagaacaa
    29281 ggacagaaaa agctaaagat cactgctaaa gacacatcga tcaatattac gacggatcaa
    29341 gaggacaatg aaacagattg atcctgtact caactggata ttttaagaaa aaacagcaaa
    29401 gatccttcca atctttgctg tttttcatcg cttaacgcac ataactatag taaaataaga
    29461 aagaaaatat ctaaaaagaa aacagggtga aagagtatat gaaggtagaa gaaggtcaaa
    29521 gagaaaagtt aaagacagaa atggaaagat tacatactta tatcactcaa ctaagccaaa
    29581 ccttctatga tccagacaaa gaaaaagtga tggttaatta cccgaacaat tcagaaggtc
    29641 gccaattaga gcaagtgtac catgaagttt tcaaacattt attgacggtg aagaaagagc
    29701 tggattatta ttctttaccg attatcgata caggaatatt gaagtatgat gggaagaagg
    29761 aacgatttat ctttaaatct gttcgggaag atttgcctct ttcagcaggg atggatctag
    29821 aggtcttggt agaagactat tttacagaag aaaagcactg ggttcgaaca aaattggatt
    29881 atttaccgca ggcagccgga gggacacagg caagcggttg gtacatcact gaagacaaag
    29941 agttagaact agaaggtgta atggcgcgca ttcgaaaaaa aattaaaaaa gtaacattag
    30001 atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag agcattttat
    30061 atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg attgtttttc
    30121 ttctatttag gcatgtggca agcgaaagtt tgggcacttt ttttaagtat ttttctttgg
    30181 ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca agcaaaagta
    30241 attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt ccttacgata
    30301 cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag ttctgtttta
    30361 aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt ctttaaagtc
    30421 gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt agaaatccaa
    30481 agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt caatgagaat
    30541 gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa agagctgcaa
    30601 gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca tttagcctat
    30661 gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat tttgtctgct
    30721 cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga gcaaatcgaa
    30781 gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa taatttactt
    30841 gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga catgaaagac
    30901 cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat tattgtcttg
    30961 aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat gatagttgat
    31021 tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga gagaagggga
    31081 aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac ttgcaggggt
    31141 gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa ttacgacaga
    31201 tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact atagatatat
    31261 cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg gacaattgac
    31321 tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata aaggtgtcac
    31381 ttcgtatgaa gaagtcgaaa aaaaagacgt tccgaaaaca gcactggaaa aactaattaa
    31441 agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa agtatacgga
    31501 aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt agaaaaaggc
    31561 gaatttattg ggatcatggg cgcttctggt tctggtaaat cgacactttt gaatatttta
    31621 tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga tgtgaccaca
    31681 ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat tttccaagac
    31741 ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct ttctttacaa
    31801 ggcgtcaaac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg tttatccatc
    31861 tctcatattt tggaaagcta tccttctgag atttctggtg gacaaaaaca acgggtcgcc
    31921 gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc tacaggtgcg
    31981 ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa tacaaaagat
    32041 catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg ccaacgtatt
    32101 ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca aagccgtgat
    32161 tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg ggagattgac
    32221 tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact acctggtgtt
    32281 attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa tggcaagcaa
    32341 cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt tccgattcgg
    32401 ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt cctttttgat
    32461 gacgatgcgg caaaaagact atgcaatgtt tatgatgcta ggagcaaaag gcagaaaaat
    32521 cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt tagtgggttc
    32581 agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc agttagatct
    32641 caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg ttttgttttt
    32701 tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca aaaaaccgat
    32761 attagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata aattcttgtt
    32821 tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata tgatgaaaca
    32881 cgtggggacc tatcaagtct ttggtatagg agtagcctta gttacgatcg tattaggaac
    32941 ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga aatcagaaca
    33001 gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc gtattcgtga
    33061 atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg gcgctttgac
    33121 ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt ctggatatga
    33181 tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac tttcaccaac
    33241 actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata atgcaagtga
    33301 atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc cgaagatcac
    33361 gaaagaaaaa gttcctgctg aaaaaatgaa tgaactgact gtacaagagg aacttcgaag
    33421 ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat cagaatttga
    33481 tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact tccaagcaaa
    33541 cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat ctttgcaaaa
    33601 agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc tattctctgg
    33661 ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg ctagctgttt
    33721 gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa tgcttgaaaa
    33781 gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag gcgtgttatt
    33841 cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa tgtttggtct
    33901 attcatgcaa aatccataca aagatatttg ggttccgttt acgatcttct tcgtattgta
    33961 tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga aaaagagatt
    34021 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag ctcctcctat
    34081 ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttgttcggaa
    34141 aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt attagaatat
    34201 agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt ccatgtatac
    34261 tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca atgaacaaaa
    34321 ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat ctttgtctgt
    34381 cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg aaaaacgaga
    34441 gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt atatcggtgc
    34501 ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag atgatgaaac
    34561 gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg cacctagctt
    34621 tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat cggtcatttt
    34681 ctatcagact agcccagaca tggaaaacgg atttcctgga actttagatg tagaagtacg
    34741 gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca gtgaccgcaa
    34801 aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc cagcacagtc
    34861 aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca ataaagatac
    34921 aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc aacgtcctaa
    34981 aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg gcggtatcga
    35041 tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat tgacaagccc
    35101 agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga tttttacagc
    35161 aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc atggcggtat
    35221 cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg gagatgtcac
    35281 tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata taaagaaata
    35341 gaataaatta aaaagggagt gtgacagtgt cacatcccct tttta
//
LOCUS       DAGCYL010000008        35259 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.008, whole genome shotgun sequence.
ACCESSION   DAGCYL010000008 DAGCYL010000000
VERSION     DAGCYL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.008"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            76..1416
                     /locus_tag="MB216_000291"
     CDS             76..1416
                     /locus_tag="MB216_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4525154.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(1565..2794)
                     /gene="rpsA"
                     /locus_tag="MB216_000292"
     CDS             complement(1565..2794)
                     /gene="rpsA"
                     /locus_tag="MB216_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBT4525155.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(2929..3609)
                     /locus_tag="MB216_000293"
     CDS             complement(2929..3609)
                     /locus_tag="MB216_000293"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBT4525156.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(3658..4293)
                     /locus_tag="MB216_000294"
     CDS             complement(3658..4293)
                     /locus_tag="MB216_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4525157.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4359..5762)
                     /locus_tag="MB216_000295"
     CDS             complement(4359..5762)
                     /locus_tag="MB216_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBT4525158.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(5749..6780)
                     /locus_tag="MB216_000296"
     CDS             complement(5749..6780)
                     /locus_tag="MB216_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4525159.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            6811..7032
                     /locus_tag="MB216_000297"
     CDS             6811..7032
                     /locus_tag="MB216_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBT4525160.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7197..7934)
                     /locus_tag="MB216_000298"
     CDS             complement(7197..7934)
                     /locus_tag="MB216_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBT4525161.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8087..9427)
                     /locus_tag="MB216_000299"
     CDS             complement(8087..9427)
                     /locus_tag="MB216_000299"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBT4525162.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(9664..11187)
                     /locus_tag="MB216_000300"
     CDS             complement(9664..11187)
                     /locus_tag="MB216_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBT4525163.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(11565..12176)
                     /locus_tag="MB216_000301"
     CDS             complement(11565..12176)
                     /locus_tag="MB216_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4525164.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12299..12424)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(12506..13222)
                     /locus_tag="MB216_000302"
     CDS             complement(12506..13222)
                     /locus_tag="MB216_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBT4525165.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13228..13797)
                     /gene="scpB"
                     /locus_tag="MB216_000303"
     CDS             complement(13228..13797)
                     /gene="scpB"
                     /locus_tag="MB216_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBT4525166.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(13781..14578)
                     /locus_tag="MB216_000304"
     CDS             complement(13781..14578)
                     /locus_tag="MB216_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBT4525167.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(14548..14919)
                     /locus_tag="MB216_000305"
     CDS             complement(14548..14919)
                     /locus_tag="MB216_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBT4525168.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(14938..15825)
                     /gene="xerD"
                     /locus_tag="MB216_000306"
     CDS             complement(14938..15825)
                     /gene="xerD"
                     /locus_tag="MB216_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBT4525169.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(15921..16394)
                     /locus_tag="MB216_000307"
     CDS             complement(15921..16394)
                     /locus_tag="MB216_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBT4525170.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(16499..17362)
                     /locus_tag="MB216_000308"
     CDS             complement(16499..17362)
                     /locus_tag="MB216_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBT4525171.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(17558..19657)
                     /locus_tag="MB216_000309"
     CDS             complement(17558..19657)
                     /locus_tag="MB216_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBT4525172.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(19877..20986)
                     /gene="rpoD"
                     /locus_tag="MB216_000310"
     CDS             complement(19877..20986)
                     /gene="rpoD"
                     /locus_tag="MB216_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBT4525173.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(21010..22851)
                     /locus_tag="MB216_000311"
     CDS             complement(21010..22851)
                     /locus_tag="MB216_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBT4525174.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(23033..25348)
                     /locus_tag="MB216_000312"
     CDS             complement(23033..25348)
                     /locus_tag="MB216_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBT4525175.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(25533..26393)
                     /locus_tag="MB216_000313"
     CDS             complement(25533..26393)
                     /locus_tag="MB216_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBT4525176.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(26452..27084)
                     /locus_tag="MB216_000314"
     CDS             complement(26452..27084)
                     /locus_tag="MB216_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBT4525177.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            27251..28012
                     /locus_tag="MB216_000315"
     CDS             27251..28012
                     /locus_tag="MB216_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4525178.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            28038..30629
                     /gene="mprF"
                     /locus_tag="MB216_000316"
     CDS             28038..30629
                     /gene="mprF"
                     /locus_tag="MB216_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBT4525179.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(30817..32004)
                     /gene="tuf"
                     /locus_tag="MB216_000317"
     CDS             complement(30817..32004)
                     /gene="tuf"
                     /locus_tag="MB216_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBT4525180.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLTE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            32372..32728
                     /locus_tag="MB216_000318"
     CDS             32372..32728
                     /locus_tag="MB216_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBT4525181.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(32969..33973)
                     /locus_tag="MB216_000319"
     CDS             complement(32969..33973)
                     /locus_tag="MB216_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBT4525182.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            34139..34990
                     /locus_tag="MB216_000320"
     CDS             34139..34990
                     /locus_tag="MB216_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBT4525183.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            35007..35252
                     /locus_tag="MB216_000321"
     CDS             35007..35252
                     /locus_tag="MB216_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525184.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         9914 a         7448 c         6118 g        11779 t
ORIGIN      
        1 ctaataaacg caaacagaca ccttgaaatc ctttagaata aagtcgacga aaaccattca
       61 aaggagtgtt tcaagatgtc ctattcagag tttaccaaag agttactaga tattctagac
      121 ctaaatctta cctttcatga agatgccttt cgcaaagagc gcattaatga cgaaacatgt
      181 tttgtttttg atggcacatt gacctatcaa ccagaggaat gcttccattg ccactatcaa
      241 aataagcaaa cgatcattaa atggggctgg aaaaaagtat ccatcttatt gaatgatgtc
      301 agtaactaca agaccattgt gcggattaac aaacagcgct tcaaatgtaa acactgtgga
      361 aagacttttt tagcagaaga ttcagttagc gatcgtcgct gctccattgc ccgcagagtc
      421 aaacaagcca ttcttgagct attaagtgag cctatttcta tgtctttgat tgcacgtatg
      481 aagcacattt ctccgaccac tgtcattcga atacttcgca gccttcggcc taaaaccgtt
      541 tctttgaacc agccgttacc agaagtggtg tgtttcgatg aattcaaatc ggtgaaaaac
      601 gtctcgggag ccatgagctt tgtcatgatg gacggcaaga cccatcaact gattgatatt
      661 gtggaaaatc ggcaactcaa tcctttacgc gactatttct tgcgctaccc tagaaaagtc
      721 cgtgaacaag tacgtctagt ggtttcagat ttctatacac cctatcgcac attggtgaaa
      781 gagtgttttc cgaatgcccg aatcgtcgcc gatcgctttc atattagcca acacatcggt
      841 cgggccttta ccaaccaccg aattcaagta atgaagacct ttaaaaaagg ggatcgtcgg
      901 tcgaagcact taaagaaata ctggagacta cttcaaaaga atgcgtggga attgaatggt
      961 cagcatcgct actggcgtcc gtcatttcga gatcatctga cagaagcgga gatagtcgat
     1021 cggctattat attatgatga ctccttaaag cgaggctacg aagtctacca ggtttttctt
     1081 tccgctatca gaagacaaga tgttcctgaa ttcgtcgcgc tcctaaaaga agactacaag
     1141 gagttgccag agcactatca accagtgttc acgaccttca agaaatatcg aacagaaatc
     1201 aaacgagcgt tgcgagttcc ctattccaac ggccctatag aatgtttgaa caaccatatc
     1261 aaagtactaa aacgaattgc ctatggcttt agaaactttc aaaactaccg agaacgaatc
     1321 tttttatatc gaggaaaata tttcaagaaa actaagaaca ctacccaact gactaaagcc
     1381 agaacaacaa cacggctaga caagatagct gtctagccgt gtgagtatct cgattattct
     1441 attgaatttc ttcaccaaca ctcattgacg aagagccatt ccttattgaa atactaatcg
     1501 cttttcaaaa gaaaaactga gacaaaagca gctgctttca tctcagtcac agatacaaaa
     1561 tcaattattt ttcagaatca tcggttgctt tttcttctac ttcttctttc aacgcatcac
     1621 ctaaaatgtc tcccatagta aatccagtat tttcttctgg taattcataa gactcaacat
     1681 tttttggttc ttcttctgaa gctggttttt cttctaaagc tttgatactc aatgcaatac
     1741 gatgttcttc tggatggatc tctaatactt tcactttcac ttcgtcacct tcatgaagca
     1801 cttcatgtgg tgtcgcaata tgtttatgtg aaatttgaga gatatggact agcccttcta
     1861 ctccagggaa cacttcaaca aaggcaccga agcttgtcaa acgtttaact gttccgtcta
     1921 atactgtgcc aactggagct tttgtatcga tttctgacca aggtccaggc tgtgtgtctt
     1981 tgattgacaa ggaaacacgt tcttgttcag gattgataga caacactttg acatttacat
     2041 catcattgac agccaataca tcacttggtt ttgctacatg actatgtgaa atctctgaga
     2101 catggactag accgtcaatt ccgcctaggt cgacaaatgc gccaaaatca gtaagacgtg
     2161 caacacgtcc actgagaaca tctccctcat ggatagttga aagaatttct tttttctttt
     2221 cttctctttc gttttccact accgctttat gcgataagat caaacgattt tctgatggtt
     2281 cgatttcgat gattttaaag ctcaatgttt tgcctttata gtctgaaaag tctgcaacaa
     2341 aatgatcttc caccattgat gcagggacaa aaccacgtac accgacgtca acgactaaac
     2401 cgcctttgac aacatttgta acaggtgctt cgatgacctt gccctcttgg aaatctcttt
     2461 cgatatcttc ccaaactttt ttagcatcta aacggcgttt tgaaagtaga tagctgccgt
     2521 tttctttatc tttgccgatc gtactgataa cgactaaatc taatacatca ccgactttta
     2581 ctacttcgct gatatcttct accggcaaag ttgataattc ttttgccgga acgactcctt
     2641 caactccagc cccttcgatt ccgacaatca cttgtttgtc ttcaatgact aaaacttcgc
     2701 ccttaacaac atcgccaact tttacttctt gtacgctttt cattgcgtct tccattgact
     2761 ggttgttctc ttgattttga tcaaattctg tcataacact tgtcctccta accaacattc
     2821 tgccgtaagt acggtactgt ttttagttta attgattatt caaaaaaaat aaagcgattt
     2881 cgttcgtttt ccttgaaaaa acaattgttt ttcatttttt ttcttaattt aaaaaagaag
     2941 ataacctttt ttcaaaacaa cagcttttat tgctgcaacg acttctggaa tcgatttgcc
     3001 agtcgtatcg atacgaacag catcctctgc ttgcttcagc ggagagacgt cccttgttga
     3061 atcataatgg tctctttcct gtatctcttt cgtcagtgtt tcaagatcag tcggaattcc
     3121 tttttcttgg ttctctttga aacgtctttc tgcacgctca cttacgcttg ctactaaaaa
     3181 gattttcact tctgcattcg gtaatacagc tgtcccgatg tctcttccgt ccatgacaac
     3241 gccaccgcgt tcccctattt ttcgttggac agcaactaat tcttctctga cttttccatg
     3301 tgcagagact tcagaaacag cttttgtgac atctggttga cggatttcta aagtcacatc
     3361 ttttgatgct atgaatacat gctgtccatc ttcttgttgc gaaaaagaga taggatactt
     3421 tttgatcagc gaaactagcc cttcttcatc tgaaaaagat acattgttcg taatagctaa
     3481 ataagttact gcgcgataca tggcaccggt atctgtataa atataattca attcttttgc
     3541 taatatcttg gcaacggtac ttttcccaga tgaagcaggc ccgtcgattg caatactgat
     3601 ttgtgtcatg atataactcc tttttctcag aaaaaaagcc gcaaagcgcg gctattatta
     3661 tttaattctt aattcttgac cagggtaaag cataaagttg tttggatcta agccattcaa
     3721 ttcaaacaat tgatcagtgg tgatccctgc acgctcagcc acttgttttg gaccttcgcc
     3781 tgcttgaaca gtcgttgttg aaccggctgc agctgatgaa gaaggtgttg tttctgctgc
     3841 attgctgctt gaatcccctg cttgataagt tgcctcactt gaggaagtcg gtgttgtctc
     3901 tgctgcaata ctgctttctg acgttacacc tgtatcttga gacacagtag attcttccgc
     3961 gattgtacta gattctactg ttgaagtctc agttgactcc tcttttgttg tacttgtaga
     4021 actttctact actgatgtcg aagtttcaga agaagttgct gctgttgcat tgttgcttga
     4081 tccatttctg atccagaaat aagcacctgc tggaatagcg atacataaga ccagtaaaat
     4141 caaaaatagc gaaagaaata atgtatttcc tttcttttgt tgtctctgag aacttcttga
     4201 tgaaacttca tcatctgtat cgtagattgg ttgttcccat ggttcttgtg tttcgttatt
     4261 ttctgtagat tgatgtctat cttttctgct cactgtttta cctcctaaac tctatcgtgt
     4321 gtattatacc ataggaaaaa gtgtcttgtc ctaaaaaatc aatgttccat gtttttttta
     4381 aataaattta gtaaaatcgt ttcccaatga agttgttcag tcttagaaaa aagcttcctc
     4441 tttggcagaa gttctgcttt tgctccatca atatcacaac aattctgcgg cttttcggag
     4501 agtttttcgc caaaataagc taagatgaat tttctgcggc agttttcttc attaatatag
     4561 cgaagcatct gctgcaggcg gaattctttt tctttttcct gacgtttgag ccgttccacc
     4621 caattttctg gtttttcaga ctgctggatc agctccatcc attttttttg cagctcatca
     4681 aagggagctt gctctgtggc atattccaaa taaagctcaa aactttgtcg ctgttctcta
     4741 gaaagttgat taaagaaata atggatacgt tcatccccac tttgataaaa aagaattgct
     4801 gcactttctt cctgatctct gcctgctcga ccgatttctt gtacatagtt ttctagactg
     4861 tctggcaaat cataatggac cacataacgg atatcagatt tatcgatccc cataccaaat
     4921 gcattcgtag caatgagaaa ctgcagctga ttttttacaa actgctgttg aagttgtctt
     4981 ctttgagctg catcaagacc accgtgatag tatccaactg taaaacgttc cctgaataaa
     5041 tgatacaatc gttcgacttc tttttttgtt gcacaataaa tgattgccgc tccttgtgct
     5101 ctttccatga agtgttcaag ttcttgttct ttttgctgcg ttttccgaac aaaaagcgag
     5161 atattttgcc gattgactga ttgtctgatc tcttttggtg gtcgatcaaa tagtacatga
     5221 cgtatatctc ttgctactaa atctgttgct gtcgccgtta gtgccagtgt gaccgggttt
     5281 cctaaaagct ttcggatttt tcctaactgt tgatattctg gacggaaatc aactccccat
     5341 tgagacacgc aatgagcctc atctactaca tataacgcaa tattttgttt tttcagatgc
     5401 tctaaaagag aaggattagt caacatttcg ggactcaaaa acaaaaattt atattgcgat
     5461 agatgacgga ggacgtatct tctttcagat tgcgaaagta aactattgaa agcagctact
     5521 cgtttctccc ctcgtttttg taattgcgtc acttgatctt ccattagaga aagcagcggt
     5581 gacacgatga tgaccagacc ttccattaaa taccccgtca attgatagca aaggcttttt
     5641 cccgttcctg taggcaaaat agcgagagta tcttctccat caagcaacgc ctgaataatt
     5701 tcttcttggc cgggacgaaa tgaatcaaat ccaaaatatt tttttaattc atttattaac
     5761 attctgtatc gcttcctttc tcaatagata gatttgatac aaacgaaatt ctccataatc
     5821 taattgtcca tctacattcc attcgctaaa gcgccactct cgcacggaat ccttatttgg
     5881 ttccaaacgt tcgattgttt ctgcagagag aatccactcg tatggaaaat catcaaaaaa
     5941 caaggcccac tcaataagat gatctgttac tgttcctctt tttaaatgac ggattgccat
     6001 gatctggtca attgtctgac cattcataaa catctgcttg gtgatcatca tgctttgatt
     6061 gaaattttgc tgtaataatg aatcgatcaa agcgtgccaa agcgaatctg gccgtttttc
     6121 gatttgtgct aacaaaaggt ggatacatct gctttgttgc agataaaccg ctgactcatc
     6181 attgtttttt gccagctgat aaggaagcag ccctgttcgc tcatttccag ataattggtt
     6241 agctaaaaaa tctgctgctt cttctggtat ctccgaaaac agctgcacca aactctcata
     6301 cgcagtatca atcaaaattt cacgtggcaa ttggctttca ctaagccatt tttttagttg
     6361 gaaagtataa aaaggacttg tctctgctgg aagatagtct tgaatacccg ttgccagatt
     6421 agaaattact tgaacagcaa atttaatgag ccgccaacta gtttcccctg ttctgccata
     6481 gcggtcaaaa cgcaaaccag tatgttctat ttgatgttcg gacaacatat cagcccctgc
     6541 aggcgttagc tttgcttcat tttcatttat ttcgatccaa ccttgctgcg caattttttg
     6601 caaaatttga taaaacttat cctgcttcaa ttctggaaaa ctgttatgga taaacaaaag
     6661 ttcgtgggta aatccataaa taagtactga agtcgttcgt tttcccacaa ggagttggta
     6721 aagagaacta atccgcagct tgtgtcctga tgaaaatagc gataaaataa aaacaatcat
     6781 attattctcc ttaagttagg atggttagaa atgtcactac tatgtaaact tgtacctgaa
     6841 cgctgtattg catgcggtct ttgtcaaatc gaagcgccag atattttcga ttatgatgaa
     6901 gaaggaatcg ttttatttgc ccaagaacca gaggctcatc aacagttcgt gatgccggaa
     6961 gaagaaacat atgtaaaaaa agcgtatcaa aaatgtccag ttcgagcgat tttattagaa
     7021 aataaaatat aaatataaac gaaactttaa aaacgtacac taagttctct aaacttgaaa
     7081 ataagccggg atctgtttct cttgttccgt gaaatcccga cttattttga aagattcatt
     7141 tgctatctat agttaattga ctagtttttt cacggaccct ataggaccca tttatattat
     7201 ttcaactttt tcagccagtt ttccaatgta ttgacgatca cagtttgctg gtctgcgtta
     7261 gaaatttctg cctctccatc tcctttttgc tcaccatagc tgccaaaacc tccatgattt
     7321 ccgccattga ttgatacata ctctgttctt tccggtaaat actgcttagc ttcttgataa
     7381 gcttcctgat tcaatacttt atcttttgtc gccgtgatcg ataaaacagg aacagctgat
     7441 tctgtcaatg ttcctttttt gtcgggataa cttgccatga agaatacccc ttttaaatgt
     7501 ttgtctgaat gattcttatt gacataacga cttgccatta cgccacctaa tgaatggcca
     7561 ccgatcacat aatcggaatt tggaaaatct tctttgacct tatccgcttt gtttccgccc
     7621 atcactgcta gatcaagtgg cattttgaca atagcgactg aaaagccgtt ttttgctaaa
     7681 tcttgcgccc aaatactata actttctggc tctacgagag caccaggata aaaaataagt
     7741 aatggagact ttccatgatt ctttttctct ccttcaaaaa ataagtaatc ttctttgttt
     7801 tttccttctt ttaccaacac ttgcgcttct gacgatggtt cgtacgtatt ttgctgtata
     7861 tagtaataac cggctccacc gagtaatcct aaaatgatga ctaagccaat tagtattcgt
     7921 ctaaatattt tcactttctc ccaccttttc tatctgagtg accccttttt cttaatcaag
     7981 tatgattata ccttaaaaaa ataagagaga aatgcaaact ttccagcatc aaataaaaaa
     8041 agacggaata tccatttgaa tatccgccct ttttgtatgt ttatttttat ttagcacata
     8101 aacgatacac agcttctgca taaatttcca ttgctttata catactactt aattcccatc
     8161 gttcattcgc ctggtgcata aagtcaggag tatcagggaa catcgcaccg aatgccacac
     8221 actggttcat cgtacgagca aaagtagcgc cgcctgatac aaacggttca gtcatgtctc
     8281 cagtaatatc gcgatatgtc cccaatagat ttttcactaa ctcactgtct agtggcacgt
     8341 ataatgaatc aaggaagtca aattcttcat aagtcaaccc atatttttct gctgttttag
     8401 ttaatttagc tactaaatca tctttcttga atgtaacagg gattctcata tctacaccga
     8461 tttttgtttg ttctggtgtg atttccaaac ttgcaaagtt catcgttagc tcgccggatt
     8521 gttcatcttc tgttttgccc acgacagctt caccagttgc attttcttga actaattttc
     8581 ctaagaaatt gattgggcca aaatcaaata cttctgaaag agcaattgcc agacgagaaa
     8641 tagcattcac tccttgtgct gcatctttcg cgtggatact tttaccaaga acgacgatgc
     8701 cttcaccttg ttcttcgaaa tcgaattcgt gttttttcaa tgcttctttt acttcagaaa
     8761 gcttttctcc agagtaagga gctgcatcag gaacaacatt caacgctcct ccagctttta
     8821 cagaaaactc gttcgttcct ggtcctgtca agtaagcttg caataagcct ttttctgcat
     8881 aaattaaagg aaattcagca tctggcgcaa atccttgcgt aattccttct tctttttcgt
     8941 tatatttttc taaacaacgc cataaatttt cttcatctgt accaaagata aaacgaatac
     9001 gtgtcttgaa ttccacgcca gcatcaatca aagctttgac ggcatacatt gccgcaatag
     9061 aaggaccttt gtcatcttgg gatccgcgac caactaacca gccgtctttt atagttggat
     9121 cgaatggttt tgtttcccaa ttattttcgt cccctgctgg aacaacgtcc atatgacaca
     9181 agataccgaa taattcatct ccagagccaa tctctgaata accgtaatag ccttctggat
     9241 ctttaaatgt tctaaatcct agcttttcac tgatttccaa tactttatcc aaagcttcaa
     9301 tcactttttt accgaaggga tgtccttgtc ctgaatctgc ttcatctagt acggaaggaa
     9361 ttcggatcaa ttcgctcaat gattctaatg cttgttcatg atgttgttct gtcacaaatt
     9421 ttttcatgat attatcctcc taatatgtta aaaagggtca tgtcaaattc ctccgacaca
     9481 acctcttctt ctcaaataat gacgttttaa gcctgctcca tcagcaagca gaatgttctt
     9541 aacaaatgat gaaagaaaaa acactcactg aatggagcta ttgacttatc cacgtgctta
     9601 tcgatagccg tttatcggtt tcttcattct tatgatagac cttgtacctg tatttgtcta
     9661 ggtttagaac attgctgcta cacctaggat cacacagatg gctacaaata atatcgcaat
     9721 taatttaccg gtaaatttcc accacactga aacatcgaaa cgagcaattg ctacagcacc
     9781 cattacgata gcagatgttg gtgtaattaa gtttactaaa ccagatgcag attgataagc
     9841 agtgattact aaatccttac cgacgccggc aaattcaccc ataggtccca tgattcccat
     9901 tgttgcagca gctaagccgg aagttgaagg aatcaagaat gacataggga tatagaaaat
     9961 aaatgtaata atgatgaata cacttgaaga taatgaactt aatccttgtt cgccccaatg
    10021 taagatggtg tctgtgatta atccattgtt cataaccact tggataccac gagcaacagc
    10081 tactacgatt gctacaccga tcaaatcaga agcaccagct aaaaattcag agacaaattc
    10141 agactctttc atgccataag tgattgctac tacgatggac attactaaga ataacatagt
    10201 aatttctggg aagtaccagt cacctaatgg aagcatgctt ttacctagaa ccgtacctag
    10261 taccggaagg tttgttaacc agtttgtcaa tgattcaaaa atcgtgaaat tcgcattcaa
    10321 ggttgtccaa ggaatcaaac tgatgatcat gataccaaaa gtaataaaga acaatacatt
    10381 aacatgtttt tgttttttcg tgattacttc ttgacgaccg attgcttcaa tatcgaaatg
    10441 cttcatatct tctttacgtt ggttgtatac taatgatttt gttggatctt tttcgatctt
    10501 tgatgcataa cgatacacat atacaataga aatagctaac aaaataacga acatcaagaa
    10561 tcgaagacca ataccttcac ccatcgaaat cccaacagct tgtgaagcta cgcctgttgc
    10621 aaaaggattt accgttgaag ctagacagcc tacttgagac cctactaata cgattgctac
    10681 agctactact gtatcaaagc ctacagccat catgactggg atcagtaaag catagaatgg
    10741 aatcgtttct tctgccattc cgtaagtaga gccgcctaat gcaaataaca gcatcaatac
    10801 tggaataagc attttttctt ttcctttgaa tttcttcaca actgaagcaa tcccggcatc
    10861 tagtgcccct gttttgttga taatacctaa aaatccacca acaaccaaaa tgaagaaaga
    10921 gattgggatt gcagcaggtg tttctttagt tagctccccc gctggtacac ctagcatccc
    10981 attgattggc gccatgaaaa tatcccacag cccttgagga ttttgttgta ctcgttcata
    11041 tgatccagcg acgatgttgc ctgcatcgtc tacactatag tgcccagctg gtataaacca
    11101 agtaagtaca gcgattactg caataataat aaataagacc gtatacgctg aaggcatttg
    11161 aaattttttc ttttttgttt gttccatctt cttcttcctt tcttttccgt tagagacaac
    11221 aattttggct ctaatgttat tttatcaaaa agagtatcag aacaaaaata ataacacgca
    11281 ttttgtaaaa cttttgttca aaaagttcgt ttttccctat tccctcctct tttttcaggt
    11341 ttttaaagct ttcttctttt tcattgtagc gtttcctttt ttttataaac aaacattttc
    11401 gtcttttaaa aggagaagaa tttcacattt gcaaagaatt gattgatttt tatgcataaa
    11461 tcaaaaaaat actcaaaaca aacaaataaa acgtttacat tattgcatgt cttgtatttt
    11521 tttccaaaaa aaaagaccaa gaaatttctt gatctttttg atatttattt gactgctgta
    11581 tgacgttgtt cgaactgatg ttgttttcga cttaaccatg gaagaagttg cgcatgcagc
    11641 actaaaaaga cagcactcac gatgaatcct ttgatcaagt tgaaaggaac gatcccaatc
    11701 agtacatagt ttgctaaaga catgcccagc atctgtgttg cgttcagccc taaaaacatt
    11761 aagtaaagag gcgtaataac gaaataattt gcgatactca taaaaatagt tagagcgatg
    11821 gttcctgtaa tgacacctaa aaatttgttt cttcctttat gctgtttctt tacactaaag
    11881 aaataataaa tcgggaatgt aaagatcgtt gttgataaga aactagcagt gtctcctacc
    11941 aaattatccg gagaaaaacc tgtagtcagc agatgtaata ctgaacgcaa gaacgctgtt
    12001 gcaactccgg ccgctggacc gaataagaac atgctgatca ataccgagat atcactaaaa
    12061 tcgatcttca aaaaactaaa agccggcatg atcggaaaag caaaatactg taaaactaat
    12121 gcgatcgcag caaacatcgc tactgcaacc attttctgta cacgggtgtt cttcatttga
    12181 aatcctcctg ttaggactcc tcttcagcga actttttgac gtttctctcc aaatgaaaaa
    12241 actctatttt ccagggaaaa tagagttaga gttatttgat caagcgtcgt caaataagct
    12301 ctatcttctt tatccagact atactgtcgg tatcggaatt tcaccgattc agctattttt
    12361 ttgcaaaata ggtcgtggac tataaccacc ggtcgggaat ttcaccctgc ccctgaagac
    12421 gaaccttata taatttttgt ttacatattc attatacata tctattcaaa aaacgcaaca
    12481 tctaacactt ataaaaagta agtgttcatt ttgagtggtt cagcaaactg ctgacttctt
    12541 ttttgttcag ttcacggtaa tccccaggac gtaggccttg taaagtcaga tctccgtatt
    12601 tttcacgttt tagtttttgc acaggaagac caactgcttg aagcatattt ttcacttgat
    12661 gattacgtcc ttcgtggatc gtcaattcca cgatactgtg gtttgtctta ggatccactg
    12721 ataaaatttg gaatttagct ggagatgttt ttcgtccttc gatctttatt ccttttgtca
    12781 aaggtgcaag catcgatttt gtagcgatcc cttttacctt tgcaacgtaa actttatcca
    12841 cttcatgttt tggatgtgtc aggcgctgag caaaatctcc atcatttgtc agtagcaaga
    12901 tccccgaagt atcgtaatcc agtcgaccta caggatagat acgttctttt accatcggca
    12961 aaagatccgt aacaactttt cttcctttgt catctgcaac agcagaaata actcctttag
    13021 gtttgtacaa taaatagtaa ccgtattctt cttgataaat tgggacttca tccacttcca
    13081 cagtgtcatg tttcccaact tttatcccta attcccttac aacttgtccg tttactttta
    13141 cacgtcctgc aaggatgaat tcttctgctt ttcgacgaga agtgatgcca gcgtgggcaa
    13201 ttactttctg caatctctcc attacaatta ttctccttta ttcattccat caaagaatag
    13261 atctggtgac acgtcttctt caagtgaaat ttccatttct tgtgtatcag gcagttcttc
    13321 taatgatttc aaaccaaaat aatctaagaa gtagccagtt gttccatata atatcggccg
    13381 acctggtcct tctactctac ctttttcttc aatcagttta tgggcagcta gacgctgtac
    13441 cgctcctgaa acctgaactc ctcgaatctg ctcgatttca gctcttgtga ttggctgttt
    13501 ataagcgatg attgctaagg tttcgacggc ggcttgagat aatgtgtttg atgtcccttg
    13561 cgcatatcgc ttaagtaagg gagccagctc ttttttagtc gttaatacat aatggctttc
    13621 cgtttcaaaa atatgcaaag ctgagtgcga gttttgagtg taagattctt ttaattcttt
    13681 cactaaactt gtgacttctg cttcgctgag atttaacagt ccgcttaatt cttcattact
    13741 gatcccttcc tcgccaacaa taaaaagaag ggcttcaagt tcacttaatt tcgtcatgtt
    13801 cggtatctcc tactgctgga taaagcataa ttgattcata gttttctttt tgttgcgcaa
    13861 tgatctgccc ttttttcatc aattctagca aagccataaa agtagtgaca acttcacttt
    13921 tagaaggtga ctcaaaaaaa gaatcgaaag tacagccatg cgcatttctt tttatacgct
    13981 cttcgataaa taccattcgt tcttcgatcg ttgtgtcatc gggcgtgatc gttttttcca
    14041 ctttttgaga aaatttctta cgttctaaga ttttctgcat ggcacggatc aaatcgattt
    14101 ttttgacttg tcctcgcttc aacggctttt cagcatccgc taacccatcg atgttcatag
    14161 gttcttttgt gtaataaaga cttcgttcct ctgctttttc tgtcagtctt tcagctgcat
    14221 atttatattt acggtattct aaaagctgag tgaccaattc ttctcgagga tcttcttccc
    14281 ataattcttc atcgtctgtg acgaattctt ggacaggaag caatgtcttg cttttgattg
    14341 ccatcaatgt agcagccatc actaagtatt ctcctgcaac agctagctcg agtgttttca
    14401 ttgtatggat ataattcata tattgctccg tgatcgtagc gatcggtata tcatatatat
    14461 cgatttccat ttgctgaatc agatgaagca gcagatcgag aggaccctcg aatacatcta
    14521 atttaatatt gatctccgtc aactcattca ctctttcttc tttttctttg ctagccattt
    14581 tccaatgtaa ggctctgtga tttcagttgc acggatggag acatccgggt acttttcctg
    14641 taattcatct aacatttgaa agcccatatt ctcaccgcga taagacggat tcaaagaaat
    14701 gtcatggatg atcagctgat cgttcgttgc ccagtaaata ccaataatcc cttgaatgtt
    14761 ggttgaacct tctggcgtaa agaaaaaaag ttcataatcg tcttcagatt cgtacgtatc
    14821 aatctccttc aacaaagaag ttttacctgt cagtttctta tggaaactca atagtcccat
    14881 agcaatttta cggttttctt tccggtaatg tgttaacatc ttctcacctt cctatgacta
    14941 agcccttgga aaatgctgct tataaacatc cgtcattctt ttcttcgtga tatgtgtata
    15001 aatctgagtc gtagagatat ccgcgtgacc taacaattcc tgtactgtcc gcaaatcagc
    15061 tccattttct agcagatgtg tggcaaagct atgccgcagc gtatgtggtg ttacttcttt
    15121 gtaaatccca gcctctcgta ctaactgctt cagatttttc cagattcctt gccgagaaag
    15181 acctttccca tgattattga caaatacatg catttcagat gcgtccttta caagtaatgg
    15241 acgtgcttcg tctaagtagc gttccagcca ttggatcgca taatcaccta aaggaatgat
    15301 ccgctcttta tctccttttc caactgtctg taccagtcca agagaaagat gaagatctcc
    15361 caatttcaat ccaatcaatt cactgacgcg catcccagta gcatacatga cttccaagat
    15421 tgcacgatct cttatcccta acgtttttgt cgtatcaggt gcttcaatca gcttttctac
    15481 ttctgtcaga gtcaatgtac ttggtaattt ctgtgctttc ttcggcgtat cgatatgttg
    15541 catcggatca tgatcagtga tacgctcttg ccgtaaaaac tgatggaatc ttctgagact
    15601 tgagatcatc cgagtaacag ttgctgtcgc ttttttttct tcatgcaact tctccagaaa
    15661 acggatgact aaaaagcgat cgacatcctg ccatgaagca attttctgct cctgtagaaa
    15721 atgcaagtac tgttccaaat cacgttcata gctttgtcgt gtattggcgg acaatccccg
    15781 ttcaatattc aagtagtgga gataatcgtt gatttgctct ttcataataa tccccctcat
    15841 gcctctctat catatcagat accgcttcat tctgatagtc tttttccata atctgagcaa
    15901 agaaaaagcc gtctagacac tcagacggac tggtttcttt tttctttttc atagcattcc
    15961 tgacagattc catggaacgt tagccgatga tcttttacta aaaaatgata acgttcttct
    16021 actacttgct ccacttcaag taaaagatcc tcttctactt cctcgatcgt gccgcagttc
    16081 aagcatagta aatgatgatg gaaatgtttg gcgccttctt ttctcaaatc ataacgggct
    16141 aatccatcat taaaacttac tttatcgaca attttcaatt ctgtcaagat ttcaagggtt
    16201 cgataaacag tagcaagtcc aatttccgga ctcttttttt tcactaaaaa gaagatttct
    16261 tctgcagata aatgctcttt ctcattttcc aaaagcacaa gcagagtagc ttctctttgc
    16321 ggtgtcagct taaaccccgc ttcatgtagc tgctgttttg tttttttcat cgccgaaata
    16381 gcattcatga ccatttactc aaccccaaca atttataata attacaaata aaaaaagata
    16441 taacactatg ttaattaaaa ttattataag ctagttaaga aaaacatgca agggattctc
    16501 aattagattt tttgactagt tttgtcacac cgttttcttg tgtaatcaat ccttgtttca
    16561 aaagattacc taatgcgcgc ttaaatgctc ctttgctgat accaaaagct ttcatgattt
    16621 cttctggatc cgatttatct gtaaagttga tttgctgatc tgcagaacgt tctaaaatgg
    16681 ttaaaatcat ttgtgcatca tctgatatag cttcatatgc tcttggcttc aatgaaagat
    16741 tcagtacacc gtctggtcgg acaccaatca ctcgaccttt tatctgctcg cctagacgag
    16801 gttcattata tcgttcagaa ggatggataa acccaagata gaaatcttcc gttaaaacgt
    16861 acgtacctgt taatttcaag cggtatgctg ttcctatcac atctttgttt ttcatttctt
    16921 ctgttccata tttgcttaaa gatttgaata ttttttcatc tgctaaactg ccccaaatac
    16981 ggtcttttgc atcaactcta agggaaatca tcaagcgatc ccctttttta ggccatagct
    17041 ctgtcattgt cggcaattca tcaagagata cgacaaagtc tttatctgga agaccgatat
    17101 cgacaaacgc acctaaatct tttcgtgtag cagtaacgat tccaaaaccg taatgaccga
    17161 tacggctttt aggaatttgt gtagtgatcc gattttcatg tttttgattt tggtaaccga
    17221 atccttcaac tgcttcaccg attttgtgtt caccttctga tttgcttaag ccaaaggttt
    17281 gtccgttttt ttggacaaag tagttttttt cattttcatc aataataata cctgtaaata
    17341 tagttgctaa taaatcgttc atttttttct ccctctatag tgaaaggttt tcttaataca
    17401 tttctttgct atcataacat aatgttatct acttgaatag ctgagccttg gtttggaaag
    17461 gtgaaaaaag gagtatgaca acagttgtgt catactcccc tttttcgaat aaacggtgtt
    17521 aaacggcagg ctcacagcct ctttcatttg cctaaaatta attgctccca tttcctccga
    17581 accatccagt tttacagttg atggttacga tgtaaaatgg caaagcactt tctccaatgc
    17641 ctttcatcaa tgcagatttt gttgaagtgg catatagatt ataatagcct tccccatttt
    17701 cgattctggc tttttctaat ctgtaacctc cttcaacgat atatccacgt tggatacatg
    17761 tagcaatcac tttgctttgg acacctggtg cccacgtcca tgctggatta gatgcatcgg
    17821 caatatcact tgctctggca gatttgattg gctgattcgc tgagtttgaa gaggaagggg
    17881 tcgaggatgc cgcttcttgc tgtgctgctt gtgcgaccgc agcttgttga gcggcttgag
    17941 ctgcagctgc tgcttgagcg gcttgttttg cttgttcttc agctgctttt ttcgcttctt
    18001 ctgctttttt cttttcctct tcttcttttt tctctttatc agtgagcatc ttatctactt
    18061 gtttcaattg ttcattcaac tgagcagata gatctgtatt tttcactttt tctactgctt
    18121 cttttgccgt cttgtattgg tcgcgtgttg ctgaatccac tacttttccg tctttaaaga
    18181 ctatagctgt tttttcttta gctgtttgca attcagcata ttgattttta gcttctgttt
    18241 gtgcttgttg aatcaatttt ccaaaatctg tgttatccgt ttcgattgct tggatctctt
    18301 tatctgcttt caacttgatg tcatctgcta gatgatcatc gacgatggca ggtgctatga
    18361 accactcatt cactttttgg atatctcgga tcttagattg gatatcttgg taagttgttt
    18421 ctaactctga atatcctttt tcattcttaa gactggataa atcatttttt agttttgata
    18481 aatcttgatg aatcatatct gcacgaataa attgatggtg atcatctgta taaaaggcag
    18541 caagtttctc atgaagttct tccatttgtt gctgtctttt tgcttctgct gctacttcgg
    18601 cttctacttt ttgatgatgg tcataataag cccagcttcc tccaccgagt agaagtactg
    18661 ctgctaaaac gagataaggt actttttttg attttttctt ttgcgcttta tctggatctg
    18721 ttttttctaa gtttgattct tgatgtgttt tttcagtatc tggcacgatt gttattgatt
    18781 tcgcttctgc ttcaaacagc ttaggctggt tctcttcttt ttctattttt gcttcaagat
    18841 cagtaccctc tttgcttaca gattgatttt cagcatcgga ttcactattt atttcatcgg
    18901 gctctgttga ctttttgtct gtgttttcga gtatttcttt tttctctgct tccggcagtt
    18961 tattcgtttt ttccttttct aattgttttt cagtaaatac ttcagttttt tctgtgatat
    19021 ccgtttttac gtcttttatt tttactgttt ctttacctgt ggcgttttct ctatcagagt
    19081 ctttttcagt ttcttgttcc ttttctggct ctactttgtt ttcctgtgca attttttcac
    19141 tgatcatttt gagcagctct ttcgcgtgct cttcttttgt atcttcacga tgtgctttga
    19201 tataagcgga tagtatcgaa ttttcttcct cattatcgac gatttctttc agttcagcta
    19261 ctcttctttt ttgttttgat tcaagcgttt cttcttgatt ctttcctgat tcttccgaag
    19321 tctcatgaac agttgctttc ttctgttcat ttttctctat attagttact tgatcgtgag
    19381 caggtggttc ttcgtcttct ggttctgctt tttcgaataa ttccattaca gattctagag
    19441 gtaaatcttt caattccgac cattcgattg gatcgtttaa ttctgtatca ggaaagactg
    19501 catcttcagt tgatttatcc tgcatagctg catctgtatt tgtttcttca attgattctt
    19561 ttatttgctg atcttctgca gttatttgat gaccgcagtc tggacagata gtattctgct
    19621 ccattttttt cgatccgcaa tttggacatt ttttcatcac ttagcgatcg gctcctttac
    19681 taggtttacg ctaccattta atctatcata ttctatacgt cacgccaata gcttattttt
    19741 aggatttact taaaaataat gaatgaattt ccttcctata gaaaaaaaag cttgagactg
    19801 cgtagtctca agcttttttt cttttaaaat aaaccaaaca gttttactga tttctcttct
    19861 ggtcaatggt ccacgcttat tctaagaaat cttttaattg ttttgaacga gaaggatggc
    19921 gcaattttct taatgcttta gcctcgatct gacgaatacg ttcccgagta acaccaaaca
    19981 ctttccctac ttcttccaat gttctcgtcc ggccatcatc taaaccaaaa cgtaagcgaa
    20041 ggacattctc ttcccgatca gtcaatgtat ctagtacatc ttccaattgt tcttttagta
    20101 actcataagc tgcgtgttct gctggacttg ttgcttcctg gtcttctatg aaatcaccta
    20161 agtgagagtc atcttcttca ccgattggcg tttcaaggga aacaggttct tgcgcgattt
    20221 tcaagatttc tcggactttt tctgttggaa gatccatttc cgcaccgatt tcttctggcg
    20281 taggttctct tccaaggtct tgcagcaatt gacgttggat acgtatcaat ttattaattg
    20341 tttcaaccat atgaactgga atacggatcg tacgtgcttg gtcagcgatt gctcgagtga
    20401 tggcttgacg aatccaccat gtagcatagg ttgaaaactt aaatcctttg cggtagtcaa
    20461 atttttcaac tgccttcatc agtcccatgt ttccttcttg gatcaagtca aggaactgca
    20521 tgcctcgtcc tacataacgt ttagcgatac ttactactaa acgaaggttt gcttctgcca
    20581 gacgctgttt cgcttcttgg tcgccttctt cgattttcaa tgctaacgct acttcttctt
    20641 ccgcagttaa aagggagaca cggccaattt ctttcagata catacgaacc gggtcattga
    20701 tttttacacc ggttggcgca gaaagatctt ctgtttttgc ttgttcagct tttttctcag
    20761 cactcttcaa gctgtgaatt gatggatcac cattttcatc caccacactg atacctgcgt
    20821 cttcaatctt ttggatcaat ttgtccattt catctgcatt caaagtgaat ggtgtagcaa
    20881 gctgattgga taaatcatca tatacaactt gtcgtttggg tttgttttct ttgatgaaag
    20941 ctgctactgc tgcttcatac gtttttttat ctgttccatt tgccataaaa gaaggccccc
    21001 cttgagtagc tatttggctt gttttaactg tttcgttaaa ttgatgattt caatcgtcaa
    21061 ttcaagctca agctgtttgt ttccagttcg tctggcttct tgctgcattt gtttttttaa
    21121 tgttatttca tcagctaagc cagatttgct gatcacatct agcaagtctt ggatttctcg
    21181 tggcgaactt tcttctgcca ccggtaaata ttcgatttcc accaccaacc gccttaaatt
    21241 ttcatcttgc aaaaagtcta aaaactctgc aaggttgaat tcataatgtt gtgagacata
    21301 tgcatcgaat aaaaaataaa cttctgcata tgcatcatga ataaactgaa attctcgttc
    21361 ttttaaaata ttacggacag aaacttcttt aaatgcacga tataatagaa tctgctctgc
    21421 cttttctact tgcgtcaaag gtctcttttt ccgtgcagtt tcttgtctcg taacctgagt
    21481 gtgttgactt tcctgaggtt cttgacgatt ttgctgattt gtccttcgaa gatcgcgcag
    21541 ttgtttttgt atcgtctctc tgccgatctg aaactctgtt gacagttgag atagatacat
    21601 atcttgttcg attggtgaaa caactcggct aagttcaact agtaattctt cgaggtattc
    21661 gatcttgtct ttttcattat ctaaattttt cccttgtttt aaataacgtg atttaaaagt
    21721 aaagactgtt tcgcgcccat gagccactaa ttcttggaaa gatgcttctc catacttacg
    21781 gagatattca tcaggatcaa gcttttctgg aacgactacg acagaaagtg ctaatcgact
    21841 gttttgccga agcaattcaa tccctcgatt ggttgcttca aaacctgcgt tgtctccgtc
    21901 atagcaaaag accaattctt tcgtcagctt ttccatttgt tggatctgtt gagctgttag
    21961 acttgtcccc atagaagcta atccagtttt gataccagcc tgccatgcag caatcacatc
    22021 cataaaccct tcgaacaaca aaatttctcc gtttttccgg atatcgcttc ttgctttatc
    22081 aagattaaat aaaacttgtc ttttattgaa cagttctgtt tctggactat tcaagtattt
    22141 gggctgatct ttttcctgcc ctttttcagg tgcaagccag cgtcctgaaa aaccgatcgt
    22201 ctggccttga gaattacgaa gtggaaacat cactctttga taaaaacgat cagacatacg
    22261 gccatcatca tgcgtgacaa acaatccaga gcgttctaac aattcatttg aaattgattc
    22321 attcttgaat acttgctgaa gaaaagaacg ctcattaggt gcaaaaccta tgttgaattc
    22381 ttcaattaat tctaaagaca acccacgact ttgcaaatat tctaaagctg gctgtccagc
    22441 ttttgtatgc aatagcatgt gatgatagat ttccgctgct ttttcatgag cagcaatcag
    22501 tttccccgta gcagattcag gattttggac agctatagta cgctgacgcc attgatcttc
    22561 taacggtatc tgttcgaagt ctgccacttt taccactgct tctggaaaac ttagaccttc
    22621 tagttcttga agaaaagtaa acacgtttcc gcctttacca caaccaaagc aatgaaaaat
    22681 ctgcttgtct tctgcaacag aaaaagacgg tgtacgttct tcatgaaaag gacacagccc
    22741 caagtaattt ttaccagact ttttcaactg gacatattgt ccaatcacct caacgatatt
    22801 cgtttgactg cgtactgttt cgataacctc ttgaggaatc cgttgagcca ttttttcacc
    22861 ccctgctcta ttgatatctt ttgggtttaa cagaagatat ttcaggagaa taaccggtaa
    22921 aaaatcgcac taactgaaat cagtgcgatt ttaaaggaca caattataca tgtttgattg
    22981 tatcatattt cattgctttt tcaagtataa cacatttcta tctcattaag catcaatttg
    23041 ctactctttt cagtaactga ttttgaatat cagccttcca gtacaatact cgaagaacaa
    23101 tatttacgag catgaataca aagaagtatc ctacgctctc tcccaaccct aagtttactt
    23161 ccaaaattcc atggaacagc attgcagcta catatatccc tacgatcgtc gttactgcta
    23221 ggaagatgcg caatgggttg aagggataac aagcttttac aactgccatg caactgatcc
    23281 caatcagcaa gtaatacatc aaagtagtag tttcagcatt tccccatgcg aatgtttttc
    23341 caagtagata caccgcaatg atattcacta ctacaagcaa ggcatttggt aaagcattaa
    23401 taagtgctgt aggcaaataa cggtaagtga tttttctttt atcttgttca aaagataaaa
    23461 agaaggaagg atacccttca atcgctaaat caatcagcgt gatttggata gggataaacg
    23521 gaaatccaat tgctgtaacg gcacatataa tcgataaaat aaatgaataa atcgttttga
    23581 taaaaaagat gcctgagact ttcgtcacat tgttgaccac tcggcgtcct tcaaacagga
    23641 cttctggcaa tgtagtaaaa tcagagtcca acaataccag attcgcaatc tgacgtgttg
    23701 ctccatcacc ttctgccata gcaatgctgc aatctgcttc tcttaacgcc aaaacatcat
    23761 tcacaccgtc gcctgtcatt gcaaccgttc tgccgctttc tttcaattca tttaccagca
    23821 atttcttttg ctgcggactc actcgtccaa aaaccgtata gcgatgagca gcttctcgca
    23881 cttccatttc ttcagtaatt tgagaaagat cgacatacgc atcataattc ggtaatcctg
    23941 cacgtctagc gatattggag acagtaaaag gattgtctcc agaaatcact ttcagatcca
    24001 caccttcctc atgaagataa gctagtgttt cttgagcgtt ttttcggatt ggatcatcga
    24061 tttcaaacag agcaaccggc tcaacaaatg ctggtttttc ttcgttcaat tgctgatttg
    24121 gagcgatccc caacataagt acgcgatagc cattcgcttg ggctgttttt agactgtccg
    24181 gaagccgatc ttcttccacc aaacgttcag gcgctcctac gtacacgact cccagttctg
    24241 gaaattccat cgcgccccat ttgcgatcag aagaaaatgc gactttgttt gtcacatcat
    24301 accgattaga aagctcaaaa taatctctca tagcccgcat agtgatgttg ttatcactgc
    24361 tttctgccag atacgtcccc ataatttctg ggatggcttt ctcgtacagc ggatgtagtg
    24421 cttctacccg ctggatactc attttccctt cagtaatggt ccctgtttta tccaagcata
    24481 atgtatcgac atgggccagc gtttcaattg aatacatgtc ttgaacaagg attcgttttt
    24541 ttgccagttt ggttacccct gtcgttaatg caatacttat tagcaatacc aaaccctttg
    24601 gaagcatgcc taacaaagct gctgcagatg caataacaga aattttgata gaagcatcgc
    24661 ggatgaacag tgcttccaaa aatagtatga tccccaaagg aataatcacg aaactagtga
    24721 atttggatac tttgcgaatc gaatctacaa gttcagaacg aatagctttg tgagtctttg
    24781 cctcgctagt caagcggaca gcataattat ctgcacctac tcggataact tcagccatga
    24841 cagaaccgct tgtcaaaaag cttccagaaa gcaattcagc tccagtttct ttgacaatca
    24901 aatcagattc gcctgtcaaa agagcttcat ttgcttctgc ccgtccagat ataacctgta
    24961 aatcggctgg tatctgttct cctgctccga tttttaccaa atctcctaat accagctctt
    25021 tagtagaaat cgattgttct ttaccgtccc ggatcactag tacttctgat tgggaaacaa
    25081 tcgataattt ccgtacaaga tttcttgcat gtatttcttg ataaattcca ataacgatat
    25141 tgacgacaat aattgctaaa tacgccatgt tggaatacgc acctacaaat aacagacaaa
    25201 tcccaatacc aaagttcaga aaattgaata acgtcaatat attatcgctg aatatttgtt
    25261 ttgtactttt tgatgcatct tcctggtagt cattttgctg gccagaagcc attttctgaa
    25321 ctacctcttg ctgtgtaagt ccattcattt tctttttctc cattttccgc caactttcaa
    25381 aaataatcac gtgtttaagt ctagcgtgtt ccaagattaa aaacaacttg tttttataaa
    25441 atttatagaa aaacacacga atgattttac aaaaaagatg ccaacctttt tgtttgaatc
    25501 acccgtgtgc tgtttttatt atcaatctat ctttacttat attgttcctc caaacggctc
    25561 atccaccaac ctagtgctgt accggctata aaaagaacag gacaagctag taccccagca
    25621 actcccaatc ccgcgtaatc ggtaggaatg atcataatcg ttgaagcaaa aactacacct
    25681 aaaatgaaat ggaatagctt gctatatgcc tttgagaaaa tgtaatccat gatttttgac
    25741 aatccaagaa cacaaagaat cccaccaatt ccgatcggca caatcacttc aagtcgtact
    25801 tctttaaatc cgtcagccat cgctttatac aatcccatat acaccaaaaa attagaaggg
    25861 ctcaatcctg ggacgatcat acccaaacca atcaatccgc cggcaatcag ccatgttcca
    25921 aaattctgat cgacttgccc gtcaaataat tgagcgccgt ataataaaaa caaataagcc
    25981 agaacaaaag taacaaccat gatgatcaca tcagaagtat ctcttccttt tttccctgct
    26041 tctttccata acgcaggaac agttccagcg atacatccga caaaaaacca taatatcgtt
    26101 gtttcatacg cacctaataa aaaactgact aaaaaagaaa gtagaaagat gcctgtcact
    26161 ccgcctagac caacaggcag aaaaaacaaa acattttctt tgaaattttt tgtaaggtgg
    26221 gctaaaaaag aaatcagccg ctcatacatg ccaaagacag ctgctaatgc accaccgctg
    26281 actcctggca agataaatcc tgaaccgata aacattccct tcacaaaccg taacatccag
    26341 tctttggttg tgtaccccac ttcttgattt tccatttttt gccttttttc cactatttgc
    26401 gcactccttc tttcatttaa ttttattcat gattgaacat ttcctgctat tttacttaaa
    26461 gactttttca taggtctcta ttattttttc attcattttg acaatatcag gataaaacat
    26521 gttacctcca ttatgataga gatagccgcc attgtaaagc aaggactgga tccgccaata
    26581 tcggtattgt ttcccttcct catttcctaa aagtgggctc agcacatctt tagaatacgc
    26641 ttctgctaat tctactgtgt tttctccgcc atgatttttt acataggcaa tataatcttt
    26701 cccaaaatta tatgcttgga cagctgtcca taaatcgcat ccttgtttct ttgcttctac
    26761 tatcatttcc gccaaatatt tgacaccttg atcaatactg tcagctggat tatcaatgat
    26821 ctcagcttct ccatgcagac tttccgagct ctgcatgaga tctgtccctt ttccttttga
    26881 ttcggtcaat attatcgcct tgatcaaatt ctcgtagttt tccatatgat aattttttgc
    26941 agctgttgtt atttctattt gatagctttc cactcgtttt accgaacgat aagttcttcc
    27001 tcctaaaaac aacaccgcac caataaccgt cagaatgatc atgtaaaaaa gaatacggat
    27061 aatagataat cgttttcgct tcatttcatc acctagttat tttgacatct ttttttatct
    27121 tactatttct ttatcttctt ggctatttct tccttgattt ctgtgtacgc cttaagaaaa
    27181 aatgaatttt cctgtatttt ttgacatctt acgaaaatga tcgttgtttt tcagtaaaaa
    27241 caattgccca atgactgata taatcgttat actttacaca gtaggtgatt atgtgaaaaa
    27301 agaaaagaaa tatgcaaaaa tgccagatgg gagcgaaatt tactatgaaa agagcggtca
    27361 aggcttccct ctctttttac tgcatggcaa tgacggcagc ggccgctttt tttcagaaca
    27421 ggtccctgta ctcgaacgct actataccgt gtatctagta gatagccgag gacatggtcg
    27481 ttcgacaaac gaagcatcta tgttgaattt tcagttaatg gccgaggatc tgaatacgat
    27541 catgttgctg gaaaaaatcg atcaggcaga ttttcttgga tttagtgatg gagcaaatct
    27601 agcactggta tttgccagca gcttccctaa aaaagttcat cgattgattt taaattctgg
    27661 aaacacgctt gttaaaggcg ttcgtttttc tgccagagtc atcagtaata ttcattatgc
    27721 atgggtatgg ttactctctt tatttcgccc aagtcttcgc aaaaatcttt tagttatcaa
    27781 acttttgctg catgacatcg ggcttacaga aaatgattta aaaaaaatca actcccccac
    27841 tttgatcatc gtgggaaaaa aagatgtaat caagctaaaa cattctttgt atatagctaa
    27901 aactatacca aaagcttcct ttgtgcttgt gaaagaacaa ggacatgaat tagctagaaa
    27961 agatcctgaa cgcttcaatc gagaagttct tcaatttctt tctgaaacat aactattagg
    28021 aatagaaggt gatttatttg ttaaaaaata ccatacaatg gctgaaaaac cacctaggac
    28081 tgttcaaaac aatctttttg atttctgtca ttgtcattat tgtcggtgaa ttgatgtcca
    28141 tcggtaaaac tctatctatc cagcaattag gagaaacgtt tgccactata cctgtatgga
    28201 aaagcggact aatgctattg attggtctta tctctgtgtt acctatggtt ggttatgata
    28261 ttattttgaa tcggatgctt gaacaaaagc agaatcctcg ttacctgttt gagacaagct
    28321 ggctgatcaa tacgatcaac aatattgccg gtttcggcgg gtttgttagt gtggggcttc
    28381 gctctgaact atatggacaa aagaaagaga gcagaaacgt gatccaagca ttatcgaaag
    28441 ttttcttgtt tttgatggct ggactttcta tttacagtct gctttctttt ttacttgtag
    28501 tcttcacgcc agttgctcct tttttgaagc aatattggat atggttgatc ggcggcggtc
    28561 tatattttcc tgtcgtcttt ttatttacta ctttcaaaaa gaaaggattc cttggtggta
    28621 cctcacttaa aactcaagga cagctattgc tagtttcatt gttggaatgg accggtgtcc
    28681 tgtttagttt cctttctgtt ggttatttaa tggaaatccg gattgatctg tggcaaacaa
    28741 ttccattatt cattgctgct tcagtgatag gaatcgtgtc tatgattcca ggagaaattg
    28801 ggagtttcga tgtaatgatg attatcgggt tgtccgctat tggtgtgcca agagaaatag
    28861 tcgttgtgtg gatcttgctc tatcgcctgt tctactacat cattccgttt ttgattggaa
    28921 tcgtcttttt cttcaaaaat atcggttcca cttttgatca gcgttattct ggtatcccta
    28981 aacaattagc aacagaaatt gcgcataaaa tcgtggttgt cttattatac ttttctggaa
    29041 tcatgctagt actttctgcc actatcccgc aagcttttac agagtttcgc tggcttcata
    29101 gtttgaaccc attgaaattt cattttatta tccaatttcc tagtatacta ttgggatttc
    29161 ttttaatcat tatgggcaga gggatcgcag cacgtgtgaa acgagcatat ttgcctacaa
    29221 tcttcttgat tgttctagct ttactttatg ttttattaag tgattttagt ttcacaccgg
    29281 tggttttctt gtctattctg cttctgatca ttcttgcttc taaaaacgaa ttatttcggg
    29341 aacaattgat ctattcatgg gagtggcgta cgatcgatgg aatcattatt ggtgcactgt
    29401 ccttgcttta catcgttatt ggggtataca atctgccaga tttccctcat cggcgtcacc
    29461 attttatttc ctttttctta tttccttctg aaaagatttg gttttcggga ttgttggcca
    29521 tcatcgctgt tagtttcatg atcgttctat ttgttcattt ccttcaagga gaaaagaaac
    29581 agattggcga agcctttaat gaagaaaagg ccctgaagat ccttacaact tatggaggaa
    29641 actccgatag tcaattgatc ttcttaaaag ataagcggat gtttgcctat gaaaaagacg
    29701 gtgaaccaac tgttcttctc caattcgctt gcttcaataa taaatgtatt gtaatgggtg
    29761 atccttctgg taagaaagaa gattttccag aagcaatcga agcttttatc gaagaaacag
    29821 atcgactatg ctatttacca gttttctacg aaacaagcga agagattgtt atgattttgc
    29881 atgaattcgg ctacgatttc attaagatgg gggaagaagc ttatgtagac ctgaatagtt
    29941 ttacaacttc agggaaaaaa atgaagggca ctcgtgctgt cttgaatcgg atcgaacgag
    30001 aaggatttac atttgatgtc ttacaaccac cattttctac tgagcaaatg agtactttca
    30061 aaaatatctc tgataattgg ttgggttcac gaaaggaaaa agggttttct ttaggctttt
    30121 tctctgaaga ttatctacaa agagcgccta ttgcagttgt caaaaatacc caagaagaaa
    30181 ttgtcgcttt tgcaacgatc atgcctactt acacgaacaa taaagtagga acgattgatc
    30241 tgatgcgata tgattctaaa aaagcacctt ctggcagtat ggatttcttg ttcttgcatt
    30301 tgtttgccta tatgaaagaa gaacatatcc aatggttcaa tttagggatg gcccctcttt
    30361 caaatgtcgg aacttccaga aagagttttt tacaggaaag gattgcatac ttggtttacg
    30421 agtttggttc tcatttttat tctttccatg gattaagaga atataaaagc aagtacgcta
    30481 caaactgggt ctcacgttat actctatatt ctagagatag ttggattgca tatgttatga
    30541 ttgctttgct gattattgat aatgcgccag ttgagcagac acaaggtaga ataagtggtt
    30601 tgaaaaaatg gatacgaaga aagtattaaa aactgcatac cgatagatga acactctcat
    30661 gatgaataaa agagactgaa gtaaaacgaa agttcatgct tctacaatct cttcttatac
    30721 aattgaagaa caaagagcgg tccaaacaga aatcctgttt ggaccgctct tttattttaa
    30781 acatttttca ttaccaaaat attcgagtcg cgtttcttat gcttcgatct ctgtaacgat
    30841 acctgaacct actgtccgtc cgccttcacg aattgagaat gtcgttccgt tttcaacagc
    30901 gattggatgg atcaagtcaa catcaatcgt cacgttgtca ccaggcatga ccatttctgt
    30961 atcttctggc aatgtgattg ttcctgtaac atcagttgtg cggaagtaga actgtggacg
    31021 atagttgttg aagaatggag tatgacgtcc accttcttct tttgtcaaca cgtacacttc
    31081 tgctttgaat tttgtatgtg gagtaatgga acctggttta gcaagtactt gtccacgttc
    31141 gatatcgtca cgttggatcc cccgtaacaa cacgcctacg ttatccccag cttccccgta
    31201 atctaacgtt ttacggaaca tttctacacc tgttacaact gctttttgtg tttcaggttt
    31261 gatccctacg atctctacct catcaccgac acgaacagca ccacgatcaa tacgacctga
    31321 ggcaacagtt cctcgacctg taattgagaa gacgtcttcc actggcaata gtaatggttt
    31381 atctgtatca cgttctggtg ttgggatata ttcatctact gtatccatca attccataat
    31441 agcagcttca gcatctggat cgccttgcaa tgcttttaat gcggaacctt tgatcacagg
    31501 agtgtcatcg cctgggaaac catattcggt caataactcg cgaacttcca tttctaccaa
    31561 atcgatcaat tcttcatcat cgactaaatc aactttgttc aagaaaacaa tcaggtattt
    31621 tacaccaact tggcgtgaca ataaaatgtg ttcacgtgtt tgaggcatcg gaccgtcagt
    31681 tgcagaaaca actaagattg caccatccat ttgtgctgcc cctgtaatca tgtttttcac
    31741 atagtccgcg tgtcctggtg catcaatatg cgcgtagtgg cgtttttctg tttcatattc
    31801 tacgtgtgcg gtattgatcg taatcccacg ttcacgttct tctggagctg catcaatact
    31861 agcatagtct tgaggatttg ctaggccttt tttgcctaat actgtagtga tcgcagcagt
    31921 taaagtagtt ttcccatggt caacgtgtcc aatcgtacca atgttaacgt gtggtttact
    31981 tctgtcataa tgttcttttg ccataattta caaacctcct aaataatata gatcactgaa
    32041 aaatttcagc tctcaccttt ttattatagt ctttttttga ctaaatcaaa aagaatacgt
    32101 ccgacttact ttttgtaaga ttcgaactaa gcttattata cgtcaatatt ggtcaaagtc
    32161 aactaagagc actcgtctgt cttatacgcg aaaaaagtac cttttataaa taaaaaaatg
    32221 tgttttttga gaaaaacttc aagatagtcc ttaactttct tttgaagcaa taagaacaaa
    32281 atgaggaaaa gtttaaaatt cagctgttgc caacaacttt caatagacga aatcttctat
    32341 ttcgttaata ataaggaaag aggtgaagta catgaaaaaa ttaattggcg tacttgtcgt
    32401 tttaggcata tttctcggcg gaagttttgc agcttatcat tatttttacg gcggcgaagc
    32461 ttattataca aaaatcacga cgaatggcga aatgtcagct acccaagcag ataacggaga
    32521 gaaattcacg acttatacct acaagcaagc ggcttataat aaagatggtg aaaaaaaaca
    32581 ggtgacgctt cgcgaagaaa gagaacatcc attaaagatg aatgcgtatc ttaaactcaa
    32641 agtcaatcct cgtaaaggtg tattaagctg ggaagaaata aaggcttctg aagtgcctaa
    32701 aaatgcggca gaaaaaatcg ataattagta aatggtctta aaattttatt agataaaaag
    32761 aggctgggac agaagcgttt aactccaaga aataagaagg agttgcttct gcttccgccg
    32821 tttatacgtg tttagagcgt gagacaaaag tgttctttac ttttgtctca cgctcttttt
    32881 ttgttatatt cgtcttttcc gatccttctc ttgacacaat tcatttaact gatcactggt
    32941 acttgaacac ctaatattct agtcattttt ataaacgatt caatacttca tccacatttt
    33001 tgatcatcac ggatatcgta ccatccggat tattcacaaa ttcaatcaaa tctgggttgc
    33061 gatatacatc tactggtacg atcagttcga tcccgtttga tagcttgaat ttttgttttc
    33121 caaatttttt ctcagaaatt tcttgtactt cttttacagg aggtgcttct gggacaaatc
    33181 cttgctcttg tacttcttct ttaaaagcca tttttgccgt gatgttttcc ttgaagactt
    33241 tttccgcaat catctcatga tccaaccgac ctttttcttc tatcgtatca tagacagctt
    33301 ctttcaaatc agccatgata tcaaattctt ctgtctcaaa tttttttcca agatgcttgg
    33361 ctactttttg gatcactttt acattttctt ctaaagaagg tgcaggaaca ctttcgatca
    33421 ctcttccaga aaaataaagc tcttttttgc cagaaaactc atattttttt tcaattaatt
    33481 cgtaggtcca atcagtcaaa ttcacagcga atgcttcatc tgctttttgt gttttcgccc
    33541 ctagaattgc tcgattcaaa atcaagcggt tgtcgatccc tgcttcgtct gcttcaacaa
    33601 agtgagtgta agcctcacga taattgactt tcaataaaga taagtacatc actgtatcta
    33661 attcatacaa gacaacaaat acatctgcac taggtgcttc ttcactttct tggtagatat
    33721 cgaaccaccg ctgaacgatt gcttcacttg cttccacaaa attttcttga gcgattttaa
    33781 gtgattcaac gatcatgctt tcttctgcta gttgtccagt ttttgtttga gcagaagaga
    33841 ttttttggat cttcttttga agaaaatcac gaatatattc ttttgtcaga tccagctctt
    33901 tttgtgaaaa gactggagaa ccagtttctc tgtcaataat atgcagaatt gcttttttta
    33961 aatagatatc cattttctca ttcccttctg tcttagttta cggaaaaaaa atcaaaaata
    34021 ccagaatcaa aatcattttc ttttaaacaa aaatttcaag agtagaaaca aaatggattc
    34081 tttttttcga aaaatgcgct acacttatag ttgaacgaat aaaaaaggag ctgaaagtat
    34141 gaattcatta acaagcactt atcgtctatc aaatggttac gaaatcccgg tagtaggatt
    34201 cggcacatgg caaacacctg atggggatgt cgctgtttcc tctgttaaag aagcgctggc
    34261 tgcaggttat cgccatatcg atactgccca aggatacaag aatgaagaaa gcgtcggcca
    34321 agcgattaag gaaagtggaa ttcctagaga agagatattc ttgacaacaa aattatggaa
    34381 tgcaaaccac agctatgaac ttgtgatgtc ttcttttgaa gaatcattga agaagctcca
    34441 aacagattat ttagatctgt ttttgatcca ctggccaaac cctgttgctt ttcgtgataa
    34501 ttgggaacaa gcaaatgcag atacatggcg agcatttgaa gaactgtatg aagcaggtaa
    34561 aatcaaagca atcggtgtga gcaatttctt accccaccat ctagatacac tagcaaaaac
    34621 agcaaaaatt atgccgatgg tcaatcaagt tttccttgca ccaggcgaat tacagacaac
    34681 tgtagttgag tatgcaaaaa aacatgatat gatcctcgaa gcatatagtc cattaggcac
    34741 tggtaaaatc ttcgatgtgc ctgaaatgaa acaaatcgct gaagcacatg acaagacgat
    34801 tgcacaagtt gctttgcgtt ggtcgctgca acacgaattc cttcctcttc ctaagtcagt
    34861 gacaccaagc agaatcaaag aaaatactga attatttgat tttgaattaa cagaagaaga
    34921 aatgaaacaa atcgatcagt tagatggtgt agtcggcaaa gcaaaagacc cagatactac
    34981 ccaattttaa gtcgaaaggt gattttatgg atccaatcga acgcttgaat tctctctcgg
    35041 aagaagttac ccagactttt cattccgatt ttgtcttttt gatcgatgca gagaaaatcc
    35101 aacattttcc tgcaagaaac tggacacatg atcaaatcat cgaagaactg aaaaaacggt
    35161 tcgatcattc gctaatggtc aaaccctggc acgaacatga agtcatttat tcacctgaac
    35221 tacctgtttt tgccttgata ccgaaaaaat agaaagaat
//
LOCUS       DAGCYL010000009        34766 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.009, whole genome shotgun sequence.
ACCESSION   DAGCYL010000009 DAGCYL010000000
VERSION     DAGCYL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.009"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            55..492
                     /locus_tag="MB216_000322"
     CDS             55..492
                     /locus_tag="MB216_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525185.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            587..1471
                     /locus_tag="MB216_000323"
     CDS             587..1471
                     /locus_tag="MB216_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBT4525186.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1695..4862
                     /locus_tag="MB216_000324"
     CDS             1695..4862
                     /locus_tag="MB216_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525187.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4882..5427
                     /locus_tag="MB216_000325"
     CDS             4882..5427
                     /locus_tag="MB216_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HBT4525188.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKIQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5455..5910
                     /locus_tag="MB216_000326"
     CDS             5455..5910
                     /locus_tag="MB216_000326"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HBT4525189.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5907..6665
                     /locus_tag="MB216_000327"
     CDS             5907..6665
                     /locus_tag="MB216_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBT4525190.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            7166..7354
                     /locus_tag="MB216_000328"
                     /pseudo
     CDS             7166..7354
                     /locus_tag="MB216_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7400..8365
                     /locus_tag="MB216_000329"
     CDS             7400..8365
                     /locus_tag="MB216_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4525191.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            8387..8599
                     /locus_tag="MB216_000330"
     CDS             8387..8599
                     /locus_tag="MB216_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBT4525192.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            8674..9150
                     /locus_tag="MB216_000331"
     CDS             8674..9150
                     /locus_tag="MB216_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBT4525193.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            9147..10955
                     /locus_tag="MB216_000332"
     CDS             9147..10955
                     /locus_tag="MB216_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBT4525194.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            11032..11247
                     /locus_tag="MB216_000333"
     CDS             11032..11247
                     /locus_tag="MB216_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBT4525195.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            11339..12202
                     /locus_tag="MB216_000334"
     CDS             11339..12202
                     /locus_tag="MB216_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525196.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            12239..12775
                     /locus_tag="MB216_000335"
     CDS             12239..12775
                     /locus_tag="MB216_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HBT4525197.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            12789..13211
                     /locus_tag="MB216_000336"
     CDS             12789..13211
                     /locus_tag="MB216_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBT4525198.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            13141..15540
                     /locus_tag="MB216_000337"
     CDS             13141..15540
                     /locus_tag="MB216_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBT4525199.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            15572..17563
                     /locus_tag="MB216_000338"
     CDS             15572..17563
                     /locus_tag="MB216_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBT4525200.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            17586..17837
                     /locus_tag="MB216_000339"
     CDS             17586..17837
                     /locus_tag="MB216_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBT4525201.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            17827..19056
                     /locus_tag="MB216_000340"
     CDS             17827..19056
                     /locus_tag="MB216_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBT4525202.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            19053..21134
                     /locus_tag="MB216_000341"
     CDS             19053..21134
                     /locus_tag="MB216_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBT4525203.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            21283..25203
                     /locus_tag="MB216_000342"
     CDS             21283..25203
                     /locus_tag="MB216_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HBT4525204.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            25204..26148
                     /locus_tag="MB216_000343"
     CDS             25204..26148
                     /locus_tag="MB216_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525205.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRNQLSDSKQAASPKKAAARTKKNELEV"
     gene            26152..26355
                     /locus_tag="MB216_000344"
     CDS             26152..26355
                     /locus_tag="MB216_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBT4525206.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(26655..26891)
                     /locus_tag="MB216_000345"
     CDS             complement(26655..26891)
                     /locus_tag="MB216_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4525207.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(27074..27460)
                     /locus_tag="MB216_000346"
     CDS             complement(27074..27460)
                     /locus_tag="MB216_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBT4525208.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(27442..28770)
                     /locus_tag="MB216_000347"
     CDS             complement(27442..28770)
                     /locus_tag="MB216_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBT4525209.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(28731..29060)
                     /gene="mobC"
                     /locus_tag="MB216_000348"
     CDS             complement(28731..29060)
                     /gene="mobC"
                     /locus_tag="MB216_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBT4525210.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(29318..29689)
                     /locus_tag="MB216_000349"
     CDS             complement(29318..29689)
                     /locus_tag="MB216_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4525211.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            29834..30019
                     /locus_tag="MB216_000350"
     CDS             29834..30019
                     /locus_tag="MB216_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBT4525212.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            30177..30344
                     /locus_tag="MB216_000351"
     CDS             30177..30344
                     /locus_tag="MB216_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525213.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            30962..31621
                     /gene="vanR"
                     /locus_tag="MB216_000352"
     CDS             30962..31621
                     /gene="vanR"
                     /locus_tag="MB216_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HBT4525214.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDLEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            31624..32967
                     /gene="vanS"
                     /locus_tag="MB216_000353"
     CDS             31624..32967
                     /gene="vanS"
                     /locus_tag="MB216_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBT4525215.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            33138..33944
                     /gene="vanY"
                     /locus_tag="MB216_000354"
     CDS             33138..33944
                     /gene="vanY"
                     /locus_tag="MB216_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBT4525216.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            33957..34766
                     /gene="vanW"
                     /locus_tag="MB216_000355"
                     /pseudo
     CDS             33957..34766
                     /gene="vanW"
                     /locus_tag="MB216_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT         9305 a         8524 c         9108 g         7829 t
ORIGIN      
        1 gtataatctt aaataaagaa aacaatatca aaaaatgcac gtagaagtag ataaatggag
       61 tggttttgca aatttctttt atttctagaa gttattcacg tatacggatt tgtttttctc
      121 acaaataaaa aggcaggaag tacaatggat catttgtata taaaatatat tcatcaattt
      181 tccgttttgg tgttaggcac actaattata gcggggattg tcgcatttat tattcaaggc
      241 cgtgacatat taacagtaca tcaagagttt ttcagttatg catttggtat tcctctgatc
      301 gctataaggt ctgataacaa aaagtggaag aaacgctatt acattctctt gctatttttg
      361 tgtttttatc tgccacctaa gatttttact ttattaggta tacaatgcct atcattttct
      421 tttatttcct atatcattgt tttaattttc ctcagtttca tagtatggat gttttatctt
      481 gtcaaaaaat aaatgaatgt ttcatgaaca gtacttcatc acagcctaat ttttttcttt
      541 atgctatcgt tatattaaag aaatcaatca gaagggaaag aaagtgatga aaaaagtttt
      601 attagtggta aaccccagtt caggcggtga acaagcaaag gaatttgagc aattagcgat
      661 agcaaaattg gaatcggtgt ttgatgaagt ggtcgtcttg catacaaaaa aagcagggga
      721 tgcaaaaaac tttactcgcg aagcggctac ggaagggtat catagtgtgt ttgtcatggg
      781 cggagatgga acagtcaacg aaggaatcag tggaatcgct gaacaagaac atcggccaaa
      841 tttcggattt ttcccattgg gtacggtgaa tgacttggct cgagcattag ggataccgct
      901 agaacctgag gaagcgatca atcatttttc gattgaatca gtcaagccat tggatattgg
      961 aaaaataaat gacgactatt ttatgaatgt cgtcgcaatt ggctctatcc ctgaatcaat
     1021 caatgatgta gatgcggaga agaagacgaa atttggaaaa ctagcttatt tcatgtcagg
     1081 aatcaaacag ttagcttcca cacaatccta ctcgtttcac gtagaagttg atggaaagaa
     1141 ggaagaaatt gagagcagta ccttgctgat aggactaact aactcagtcg gcggttttga
     1201 aacattactt ccaaatgcaa aagtggatga tggtaagctc catttggttt atttaaaaga
     1261 ttcttctttg atcgatacgc ttaaaacatt gccagaccta ttaaaaggag tagatgaatc
     1321 tacgaaaaat ctagcctacc ttacttgtga gcaattagtt gtgtcattag ctgatgagga
     1381 agaattgacg acgaatgttg atggagatga aggggataaa ttacctgtga caatcaaagt
     1441 attgccctca catttacagg tttattgtta aaatggaggt tgtgaccgag gagttggcgg
     1501 gagaacaccg cttgttcggg ataagggact gtgacaagac ttttgtcaca gccttttttt
     1561 tgtacaaaaa agaaatttac acctaataga aaaatttttc cataaaaata catattttaa
     1621 atatgaaagt acaattggaa atagattaaa acgataaaat aaaattgtca aaacgtcaga
     1681 gaggatggtg gagaatgaat caatgtgtaa aaaagctgat tgcgtcgatt ttgtttgtct
     1741 tccctctctt gatggtgaca tccgtttttg ccgatgtaga gaaaacaaac gaatcaacgc
     1801 aatcagagaa tcaaagtttt gacaagacta ctttttcaga agaatcgatt acttctgaaa
     1861 ctacagaatc tcaaagtgag gcttctacta ataaaaggca agaagaaaat aagacaagtg
     1921 agacctcaga agtagctgaa caaacgtctg atagcagtga agcggcactg gcaagagctg
     1981 tttcagcgga tgggtacagc gaagctgcca caacagaaga actggcgcag cttctcgcag
     2041 acgaatcagt aactaagatc cgactgattc agcctttgac tttagacagg gaattagaga
     2101 tcaaacgaga tatcgttatt gattttggtg gattcgctca caatttcggc acacaccata
     2161 tttatatcaa tgagatacct cagtcgatcg agttccagaa ttttaaagga acagctgcac
     2221 atccaggtgg attgacacca aatatcgatg gaaatgccat tatcattgct tatatgaccg
     2281 atttttgggg aagcagatat cattttacgg gagaaataaa atttaccggg acacttgatt
     2341 tgtatgatgg gtccaaatta ggactgattt acgctcctcg tgcaactgtc acattagatg
     2401 gtgtttccgg tgtgttggat gtccaaccag tgaaggaagg actgaatgcc gctccgggta
     2461 aagcctattt ttgtagaagc tatcaattga atgtgatcaa tggtagtcaa ctttatggac
     2521 cgtatctagg gaaattctac ggttttctag attcaggaga tgagactggt agtggaaaaa
     2581 cagcgccagg aatccatatc ttaagcggtt caaaggtctc tcttgattat gatcgggcag
     2641 atggcgttag cgatgaagga gaggcagtcg atacgctacc tggtaatgtc atgtttgaag
     2701 tcagtggttc gggaagcgaa ttttcggtaa atacaaagat caatattacc aatgacaaca
     2761 atcgcggaat catccaaatg agaggttcag gatcgaaagt cactgtatcg aatggtggga
     2821 aaatcaccat caatacagca actacgggcg gatttcgctt gcaaggagat tctagtcaaa
     2881 tccatgtatc aagtggtgga caaatctcgg tcaaaatggc tggagatgac gatcaacccg
     2941 ggaacaatgg gatgcgtttt gtaggaaaag gcctttcttt gatcgtagaa ggagccggat
     3001 cagttatcga catcgataag cagagtgggc gttccagcgc agttcgtttt gagaatggta
     3061 cgcaaaagct gacagtaacc aatggaggct cattgagggt aagaaataca ggtgatggca
     3121 aatcttacgt gaacagactc aatcaaggaa atcaagcgat ccaattttat gacgcttctg
     3181 gttttctaga aagtccaggt tcagctgatt ttacagtcac tggagaaaaa tcaaatatcg
     3241 atatacgcgc agattccgga gcaactgtag atacaactgg agatattgat ttaaatttta
     3301 cggcaggtga aaaaacttat ttagtcatgg tcgggaaaac aggggaagat aagaccggca
     3361 tcatcagtgg ggatgtgttg aatgtggact tagaatctcc tacctatttt gatttccaaa
     3421 acaaacggat tggaagcgag accaatggca taggcggctg ggtattccaa ggaaatggag
     3481 attcttcttt aaagatcgcc cattcagaaa tcgctttatg gcgaaaaaca ggcgcaacat
     3541 tcgataactt tgaaaaagac ccggattatt tttctgatat gacagactta tcgatcaatg
     3601 ggaccgactt aggcaatttc gtatcaagcc ctagtgcagg tttaacaaat gaatttgctc
     3661 agacaggtgc cggaatgaaa aattataatc gaatcagtgc caataatcag ctcccgatga
     3721 ttgaatcctt aagagtgcca acgaatgcag ataaaaaaat ctatgggcat gtcgttgttc
     3781 ccgaaggtgt cgaggcaaag ccaagagatg cttggacagg cgaagtagaa gtgactttac
     3841 gtattactta cgcagatacg acgaaatcat ctgtagaaat tactgctctc acgcaaggac
     3901 aaacagatga aaatgaaggg gcaggatata acccatgggg agaaggcgaa caaggcggct
     3961 tgttcgaagt tcaagtaccc aatgatgaga gattaaaaac ggaggatcaa gtgcaagttg
     4021 ttgctgctaa aaaagtaaga ggtggccaag aagtgacaga actggactct gccaaaacga
     4081 cagtcgatgt gattcctcct gaacctgcta atttaagtac cacattgatc aatgcagctt
     4141 ctcgcacgat cacaggtaca ggtgaagcag gagccttggt ttcattgaaa aaaaatcaag
     4201 ctgttttgtc agaaacaact attgatgata caggggaatt tgttctttct atcccagaaa
     4261 atcaactttt tgcaggagat gtattagaaa ttgtattaaa cgaccaagca ggagaagcta
     4321 ctgctaaggg tgtgcagaac aagccaagta ccaacgatga aattgggaat cacaatccaa
     4381 gtgatacgcc agttgtttat catgatgccc cgccttttgc acctgctacg aagatcgtag
     4441 tagaaggtgg tctctcattt cttgcccctt ccaaactcga ttttggtcag atcaaagcaa
     4501 ctggtttgac acaagaagca tttggccaaa tggatataca ggaaaaatta tggatttatg
     4561 atcagcgaga aacgaaaaat ccgtggatct taacgataaa attatctcag cctttcacaa
     4621 atgatgcagg attttctatg caggatgtcc tatatttcaa gaaaggagag gagtatcaat
     4681 tagtgaatga agaagaactg gaaattgtca gaggacaaaa ttcaacgaat gtgcaagcag
     4741 attattcaga atatttaaat aacggtcggg cgtttaaact tgtcttaacc aaagaaaatc
     4801 agatagctgg taattactca gcagagataa catggacatt agcagatgcg ccgacagaat
     4861 aaagatcaag gaggatgatt gatgaatcga atatggaata aaagtcatcg aaacaactgc
     4921 tctttactta tgatccttct ccttggattc atcctttggc agacacctgt ccatgcctcc
     4981 gaattcaatt ttgcggtaga ttttgaatta ccggctaatc agattgataa gaacaaaaca
     5041 tatataaatg taaggatgga gcctgggaaa atacaggaaa tcccctacac ggtctttaac
     5101 cgaacagata aatcagtaac agttgagaca ttgatccact ccgctacgac gaattcaaac
     5161 ggtgtgatcg aatatggaga atcaaaaagt aaatttgatg aaacattaag ttatgatatg
     5221 aaaaacttag caaaaacagt gggagaaata actgttcctc ctcaaagtgc ggtcactgag
     5281 acactttcac tgaaagcgcc agaaaaagag ctggctggtg tggtcgcagg agggatcacg
     5341 tttcaagaaa aaaaagagaa cgtttctaac aaaagtacag ttggtgtcgt caatcagttt
     5401 tctcaagtga aagcaatctt gatttgaaat agcgaaaaag agtcttcccc agatttgaaa
     5461 ttaaaaaaag cttatgcgga tcaaagcgat ggaagaaatg tgattaaggc gaatcttcaa
     5521 aatccaaatg cttcttatat gttcggcttg actacatcgg ttgaaatcta caaaaaagaa
     5581 acaaaagagc cttacctttc aataaaaaaa gaaaaaatag atgtagcacc caatacaaat
     5641 tttgatttac ttgtcccatt agaaggaaaa aaattagctc cgggacatta tcaagcggtt
     5701 gtgaagggtg cttggaaaga aaaacgctgg aatctccata ccgattttga aataactgat
     5761 gatgcagcgg accaattgaa tgaaaaggat attgatttaa aaaatcagcc agaaacattt
     5821 gactggcgat ggctgatact tgttggagtc attcttattg gcttatttgc tggattattt
     5881 attttaaaag aaagaaggaa aaagaaatga aaaaaacaat tgcattaagt accgttctag
     5941 ctggaagtct attattatgt acacaaagtg tttttgctga gaataagagc tcagccacta
     6001 cagaaggaac gatttctgtg accaaaccag atgctgcaaa tcctgaagac gttgttccag
     6061 ttcctcctgt tgatccaaat gatcctgata ctccttttac acctgacgaa ccaaatcagg
     6121 gaacatccgg attactgacg attgaccaag ctccagatct tgattttgga acaatcaagt
     6181 taggtacagc aaaaacagta tatgctgcat tacaaagcgg aacggacagt actggagcac
     6241 aaacacaagt acagaatttc gtacaagtaa cggataaaag cggaaattat gccggttgga
     6301 aattatcagt caaacgaaca gaatttattg gtacaacatc tgatacgaat aaactaactg
     6361 gttcaaaggt gaaattcaaa aacgccgtga ttcgaaatag cgcaactaat agcagtggaa
     6421 tgccatctac cgtttctgga actggcgctg aaggaatcga aatcccagca gatcaggcag
     6481 tagatctagt aactgcagga gcaaatgaag ggattggcac atggatttat tcattaggag
     6541 agaatgtgga acaagggaga caatcagtgg aattatttgt tcctgtatcc aattatgctg
     6601 tagacaacta cgtttcaaca ttcacatggt cgttaacaga tgctcctaca gaagaggaag
     6661 cgtaaaatca tatcaacgat ttcagggagt ggagccgatt caaggcccac tccctgaaat
     6721 ctgctatgct tacgtctcag ctcttattca tcaactcgaa gcagtttttt ggaagtcatg
     6781 gaaacgttcc tttgtctttt caaagatctt tcgaccaacg agtaaggagt caccgttgaa
     6841 aaattctaac acataatgag cagaatcaat tttctgcaat tgcgataagt tgatacagta
     6901 agattttaaa agtaaaaatt ttaggttatc gctgaaagcc ccggaaatac gggcatttcg
     6961 caggatatga gaactcaatt tactaccaat ttactacttt cggattgagc cgcatatatg
     7021 ctgaaccata ctgctaatac ccgccacccc aaatacggca catcataaat tgaatacgac
     7081 accgtaaacg cggcgttcct ctttcccgtc atgggagaac aggacgccgc ttttttatgc
     7141 ccaaatagag aaaggaggcg acgccatgct atttacttcc ggccctgacc gggcgtttga
     7201 acggctgatg caggaaaagc ccggtttcga tcactacgac ggcggcgggg cggacgcgcc
     7261 ggaggattgc ggcacctgcc gattttaccg cccggattgg aaatatcaat tctgccagtt
     7321 tgcagagtgt ccctatcagc cgggcaagct gaccgactat gcagcggtca aattcacagt
     7381 gaaaggagcg aacaacgcta tggcagtttt ccgtgtggaa aagaacaaag gctatacggt
     7441 gatgtcaaat caccacctgc ggaataagaa cctgacctta aaggccaagg gcctgctgtc
     7501 gcagatgttg tccttgccgg aggattggga ctatacccta aaaggactgt cctatatcaa
     7561 ccgggaaagc atcgacgcga tcaggacagc ggtatgggag cttgaaaaag ctggatatat
     7621 ccggcgtgag cagggccgtg acgcaaaagg caaaatggcc gatatggtct ataccatcta
     7681 cgaacagccg gtattggaaa acccggtatt ggaaaatcca acatcggata acccggtgtt
     7741 ggaaaatccg acatcggata atccggcgtc ggaaaatcca acgcaattaa ataaagaagt
     7801 acaaagaact gacttatcaa aaaaagaaaa aataattaca gatggacaaa gtacccattc
     7861 cattcctatc ccatccccga cccctgcccc tatggagggc gaggcagcgg ctacgccgcc
     7921 ggaacggaaa aggacaggaa aggatgaggc tgtacggatt tatcgggaaa tcattttgga
     7981 gaacatcgag tatgcctatc tcattcagga caggagcatt gaccgtgaac agcttgacga
     8041 aatcgtagac ctgatgctgg aaaccgtctg caccgcccga aagacaatcc gtgtcgccgg
     8101 ggacgactac cctgccgagc tggtgaaatc caagtacatg aagctgaccg gcgaacatat
     8161 ccgctttgtc cttgactgtc tgcgggagaa cacaacgaaa gtccgcaata tcaaacaata
     8221 cctgcgggct atgctgttca acgcccccag caccatttcc aactactaca cggcccttgt
     8281 cgcgcacgac atggcacagc ccgattgggg aaagcctaaa tccggcctgc cggactattc
     8341 ctgcggcgag ggcgaaagcc tgtaacgaga aaaggaggac gaccctatga agcagggcgc
     8401 attgattttt gacgagcgaa cagaccgtta cgacatccgc tttgatctgg ccgactatta
     8461 cggcggcctg cattgcgggg agtgtttcga cgtgatgatc ggcggcaaat ggaggcccac
     8521 ccgcattgaa atggccgaaa actggtatct tgtcggcatc cgggccgacg acctttccgg
     8581 cctgcgggtg cggatttgac ctatatctac ctgcggcgtc tggtggttcc gccttagacg
     8641 ccgcttttga ctttctgaca aggagggatg cgattgcagg aagaagttac tcaaaagacc
     8701 attgccctgt ccatgaaaac gggcaagctc acggctcaag tgctgcaagc ggcgctgaaa
     8761 aagtacctgc aacaccgggc caagggcaaa aacacgctgc accacggcca gcagagccta
     8821 aagcagctta aaaagcatgg ggctgatctc tccaacattg agatcaccga ggccaacatt
     8881 ggagcgttca agccatgcgc caagaaatac ggcgtggatt ttaccttacg aaaagataag
     8941 accacccagc cgccccacta cgttgtgatt ttcaaggcca aggatgcaga taaccttgat
     9001 ctggcgtttc gggagtttac cgccaagacg ctttccaagg agcagcggcc ctctatccgc
     9061 aaggttttgt ccgctgtgaa gcaaaaggcg gcggcccaga ccaagcagcg ggccaaggag
     9121 aaaatcaagg aaagggggct ggaactgtga agcctgacgt aaagaaattg ctgatcctga
     9181 acctcccgta tctgattttt gtgtacctgt ttgcgaaatg cggggaggcg taccggctgg
     9241 ccgctggtgc tgacctgtcg gggaagctcc tgcattttgc ggatggtttt gcggcggctt
     9301 ttgccaaccc gctgccaagc ctgcacctgt ttgacctgtg tatcggtatc gccggtgccg
     9361 tgatcgtgcg gctggtggtg tactgcaaga gcaagaacgt gaagaaatac cgcaaaggca
     9421 tggagtatgg cagcgcccgc tgggggacgg ccaaagacat agcgccttac atcgacccca
     9481 agtttgaaaa taatatcctg ctcacccaaa cggaacggct taccatgacc ggcaggccca
     9541 aagaccccaa gacagcgcgg aataaaaacg tgctggtgat cggtggcagc ggttcaggga
     9601 aaacgagatt tttcgtgaaa cccaacctga tgcaatgctt tccgaccacg gactatccga
     9661 cttcgtttgt ggtgacagat ccgaaaggca ctttggttct ggaaaccggc aggatgttcc
     9721 agcaggcggg ctaccgcatt aaaatcctga ataccattaa cttttccaag tccatgaagt
     9781 acaacccctt tgtctacatc cattcggaaa aggacgtgct aaagctggta aataccctga
     9841 tttccaacac caagggcgag ggggaaaagt cagcggagga tttttgggtg aagtcggaac
     9901 ggttgtttta ttcggccctg atcggctata tctggtacga ggccccagcg gaggaaatga
     9961 acttctcaac gctgctggaa atgataaacg ccagtgaagc ccgcgaggac gacccggagt
    10021 ttcaaagccc cgtcgatctc atgtttgagc gtttggagca gaaagacccc gaccattttg
    10081 cggtgcgcca gtacaagaaa ttcctgctgt ccgcaggcaa gacccgttct tccatcctga
    10141 tttcatgcgg tgccagactt gcgccctttg acatccggga ggtacgcgag ctgatggagg
    10201 acgacgaaat ggagctggac accatcgggg acgaaaagac ggtgctgtat ctcattatga
    10261 gcgatacgga cacgaccttt aatttcattc tggctatgct ccaaagccag ctaatcaacc
    10321 tgctttgtga ccgggctgat gatgtgtacg gcgggcggct gcctgtccat gtacgcctga
    10381 ttttggatga atttgccaac attggccaaa tacccaattt cgataaactc attgccacca
    10441 ttcgaagccg tgaaatatcg gcctctatta ttttgcagag ccagtcgcag ttaaaagcga
    10501 tttacaagga cgccgccgag atcatttcgg ataactgcga tagtgtgctt ttccttagtg
    10561 ggcgcggcaa aaatgccaag gagatttccg atgcgctggg gaaagaaacg attgacagtt
    10621 tcaacaccag cgagaaccgg ggaagccaaa cgtcccacgg attgaactat caaaaattag
    10681 gaaaggagtt gatgtcccaa gacgaaattg ccgtgatgga cggcggcaag tgtatcttgc
    10741 agctacgggg tgtgcgcccg tttttatcgg agaaattcga tattaccaag catccccggt
    10801 acaaatacct tgccgacgcc gacaagaaga atacctttga cgtggacaga tttttgacta
    10861 tcacgcgccg gaaacggcag caagtggtaa cgcaggatga agtattcgac ctgtatgaaa
    10921 tcgacctgtc ggacgaggac gccgccgctg aataaacagc ggcttttttc attccaacaa
    10981 caaaatcaaa aaatcattat gagccggaaa ccggcagaaa gaggtaaatt tatggcattt
    11041 tttaattccg cagttactac tttgcagact atcgtgatcg gactgggcgg cgctctgtgc
    11101 gtatggggcg gcatcaatct gttggaggga tacgggcagg acaatccggg ggcaaaatcg
    11161 cagggtgtca agcagcttgt cgctggcggc ggcgtggccc tgatcggtgt cactctggtt
    11221 cccctgctgt ccgggctgct gggctgatcc tggcctttgt ccctgtccaa acccttaact
    11281 aatcgcctcc gcgtcctccc ggccagaccg ggagggcgcg ggaaaggagg tgctacccgt
    11341 gattagtgaa atcattgaag aatggataaa aggcatcctc atggacggta tcacgggcaa
    11401 cctgtccggg ctgttcgata cggtaaatgc caaagtcgga gaaatcgccg ccgacgtggg
    11461 acgaaccccg caggcatgga acagcggtat tttcaatatg ctgcacagcc tgtcggaaac
    11521 cgtgatcgtc cccatagccg gggccatact cgcccttgtc atgtgctatg agcttatcca
    11581 gatgataacg gaaaagaaca atatgcacga ctttgacagc tccatgttct tccgctggat
    11641 attcaaatcc gcgtttgcaa tcctgatcgt aacgaacaca tggaacatcg tcatgggcgt
    11701 attcgacgcc acccaaagcg tcgtcaatca aagcgccggt gtgattatcg ggcagacatc
    11761 cattgatttt aacaccctga taccagacct tgaaagccaa ttggaggcta tgagcgtggg
    11821 cgctcttttg gggctgtggt tccaaaccct tgttgtgggc ctcaccatga atatcctttc
    11881 catctgcatt ttccttgtga cctatggccg catggtggaa atctatgttg tcacgtcgct
    11941 ggggcctatc ccccttgcca caatgggcaa cgcggaatgg cgcggcatgg gccaagggta
    12001 tctgaaatcc ctgctggcgc tgggcttcca agccttttta atcatggttg tggtgggtat
    12061 ctacgcggtg ctgatacaga atatcggttc cgcttccgac atatccggcg caatctgggg
    12121 ctgtatgggc tatacggtgc tgctgtgctt ctgcctgttc aagaccggca gtatatcaaa
    12181 ggccgtattc ggggcgcatt gatcccaaaa tccaagcata cacaggagga acttgtttat
    12241 ttcaacgtac aacatcattt acgctgaccc gccgtggcgg tatgactgca aaaacgtaca
    12301 gggcgcagcg gaaaatcact attccaccat gagcattgac gagctgtgcg ccctgccggt
    12361 ggaacggctg gcgtccaaag actgcctgct gtttttgtgg gccacattcc ccatgctgcc
    12421 ggaagctctg cggttaatca aagcgtgggg cttttcattc aagaccgtgg cttttgtatg
    12481 gctgaaacag aaccgtaaaa gccttacatg gttttacggg ctgggccgct ggacaagggg
    12541 aaacgcggaa atctgcctgc tggcaacaca cggcaagccg aagcgtcgtt ctgcctctgt
    12601 ccatcagttt attatcagcc cgattgaaca acacagcaaa aagccggatg tgacccgtga
    12661 aaaaatcgta gagctggcgg gcgatttgcc ccgcgtggag ctgtttgcca gacagaaaac
    12721 ccccggctgg gacgtgtggg ggaacgaggt ggaatgtgac cttatcctga cgtgaaagga
    12781 ggtgccaaat ggcttatgta accgtcccca aggacttaac ccatgtgaaa agcaaggttg
    12841 tgtttgggct taccaaacgg caactgatct gtttttccgg ggcgctggtg atcggcgtcc
    12901 ccttatattt tctcacccgt gactatctta ccaacagcgc ggcggccctg ctcatggtat
    12961 ttgccatgct gccgggcttt ttgcttgcca tgtacgagcg tcacggccag ccccttgaag
    13021 tggtgattat gcagatcatc cagtgctgct tcatccagcc aaaggagcgt ccctatcaga
    13081 ccgacaacac ctacaccgcc cttgtgcggc aataccagat ggaacaggag gtaaaggcca
    13141 ttgtccaaaa aggaaatccc aagagaaacc gaaaaaacaa agctgacccg cgcccagcga
    13201 aaagaaattg acgcggtaat ccgtaaatat aagggcgacg gcaggcccca cacggcgcag
    13261 cagagcatcc cctacgaggt catgtacccg gacggcgtgt gccgggtatc gcccggcgtc
    13321 ttttccaagt gcattgagtt tgcggacatc agctatcagc ttgcccagcc ggacacgcag
    13381 acggctatct ttgaaaaact gtgcgacctg tataactatg tggacgcctc cattcatatc
    13441 cagttttctt tcctcaatcg gaaagttgac ccggtgcagt acgccaagag ctttgagatc
    13501 gcgccgcagg gggacgactt cgacgacatc cgggccgagt acaccggcat tctgcaaaag
    13561 cagcttgcaa acggcaacaa cggcatggta aagaccaaat acctgacatt caccatcgag
    13621 gccgaaagcg tgaaagcggc gcgagccaga ttgaagcgca tcggctttga cctgttaggc
    13681 tatttcaaga gcatgggggc cgtagcccat gtgatggacg gctgggaacg gctgaacctg
    13741 ctgcatggcg tgtaccaccc ggacggcgag atattcaatt ttgactggaa gtggctggcc
    13801 ccgtccggcc tctccaccaa ggattttatt gccccgtcct ccctgtgctt cggcaatgca
    13861 aagacattcg gcatgggcgg caaatacggc gcggtaagtt tcctgcaaat cctgtccccg
    13921 gagctgtcgg acgatatgct ggccgacttc ctcaatacgg aaagcggtgt tttagtcaat
    13981 ctccatgtgc aggccattga acagaccaag gcgatcaaga ccatcaagcg caaaattacc
    14041 gaccttgacg ctatgaagat tgcggagcag aaaaaggccg tccgcagcgg ttacgatatg
    14101 gacattcttc ccagcgacct tgccacctac ggcgaggacg ccaagaagct gctgacaaag
    14161 ttgcagaccc gcaatgaacg gctgttccag ttgacgttcc ttgtcctcaa cgtggccgac
    14221 accaagcaaa agctgaataa cgacgtgttc caagccgcag gcgtggccca aaagcataac
    14281 tgtccccttg tccggctgga ctaccagcag gaacagggcc ttgcaagcag cctgccgctg
    14341 ggcgtcaatc agatcaagat acagcgcagc cttacgactt ccagcgtggc cgtgttcgtc
    14401 ccctttgtga cgcaggagct ttttcagggc ggcgcggcca tgtattacgg catcaacgcc
    14461 aagtcccgca acatgattat gctggacagg aagcaggccc gttgccccaa tgccttgaag
    14521 ctcggcaccc ccggcagcgg caaatccatg agctgtaaat ccgagatcgt cagtgtgttc
    14581 ttaacgacgc ctgacgatat ttttatttca gacccggagg ccgaatatta cccccttgtc
    14641 aaacggctcc acggacaggt aatccggctg tcgcctacca gcaaggactt tgtgaacccg
    14701 ctggacatca acctgaatta ttcggaggac gataacccgc tggccctgaa atccgacttt
    14761 gtgctgtcgt tctgtgagct tgtcatgggc ggcaagaacg ggctggaagc cattgaaaag
    14821 accgtgatag accgggctgt gcgcgtgatc taccggccct atctggcaga cccccgcccg
    14881 gagaatatgc cgatcctgtc cgacctccac aaagcgttgt tagatcagca tgtcccggag
    14941 gctgaccggg tggcgcaggc ccttgacctg tatgtgtccg gctccctgaa cgtatttaac
    15001 caccgtacca acgtggacat cggcaaccgg cttgtgtcct ttgacattaa ggagctgggc
    15061 aagcagctca aaaagctggg gatgctcatt gtccaagacc agatatgggg gcgcgtcacg
    15121 gccaaccgaa gccaaggaaa ggccacatgg tattttgccg acgagttcca tttgcttttg
    15181 aaagaggaac agacggcggc ctattctgcg gagatttgga agcggtttag gaaatggggc
    15241 ggcattccaa caggtgccac ccagaacgtc aaagacctgc tttcctcccc ggaaattgaa
    15301 aatatccttg aaaacagcga cttcattacg ctgctcaatc aggcgtccgg ggatagaaaa
    15361 atccttgcgg aacgcttgaa tttgtccacc gaacagcaga agtacatcga caattccgaa
    15421 cccggcgagg gccttttgat ctttgaaaac gtggtgctgc cctttaccaa ccccatcccg
    15481 cacaacaccc agctttacaa gattatgacg acccgcttaa acgaggtggc gggagtatga
    15541 cccggcgacg ccacaaagga ggtgaaccac catgaaagag ctgaaagcca aggcgaaagt
    15601 gacgcaggcc atgacccgcg acgggctgac gctggaaaat caggccaccg gcgaggtgga
    15661 gaacatttcc agccgggagg ccgaacagga tttgtccgcg ccggggccgg gcggcaccgc
    15721 cgataagctg ctggaacggg ccgacgccat ccatgacggc cataaggcca agaaagccgc
    15781 gaagcaggcc ggggctgtgg ccgccgaggg ggacgctgcc ctgcaccggc catccgcccg
    15841 gctccaactg tcggaggaag aacgggccga cccggattta cagccctata tccgaaaatc
    15901 ggacaaacgg gccgataagc tggataaggc ccgcgccgcc atccccaaga agcgcgtccc
    15961 ggtcaaggaa accgtctttg acgaggcaag cggcaaagca aagaccgtcc tgcactttga
    16021 caaacaggac aaggggcctc cctccctgaa acccaatccg gcaggccgtc ctgtttccga
    16081 ggtttttcta ttcggccacg ggaaaatcca tgaagtcgag catgaaaacg tgggtgtgga
    16141 gggcggccat aagggggagg aacttgccga gcggcaggcc ggaaaagcca tccgcagcac
    16201 catccggcac cacaggctga aaccctactg ggacgcggaa aaggccgagc gcaggctatt
    16261 gtctgccaac gcggagtatt tctaccagaa atctttgcgg gataacccgg agatcgcaca
    16321 ggcggccagc aaccccattt cccggttctg gcagaaacag cagatcaagc gccagtacgc
    16381 caaggccgcc cggacagcgg gccagaccgc aggcgcggcg gcacagggcg cggccaagac
    16441 agccggggcc tccacgaaag cggccaaggc cgcagccaaa gaaagtgaac gggcggcctc
    16501 ctttgtggcc cgccactgga aaggggcgct ggccgtcctt gtgttggggc ttttgatgct
    16561 gcttatcatc ggcggcttac agtcctgcac cgccatgttc ggcagcgccg gaagcggcat
    16621 cacggcgtcc tcttactttt ccgaggatag cgatatgttg gccgccgagg acgcctacgc
    16681 ggagctggaa gccgagctgc aagactatct ggacaactac gaggccaccc atgactatga
    16741 cgaataccat tttgaccttg acgagatcag ccatgacccc tatgtgctga tttccattct
    16801 gtcggcgctc catgacggga tatttaccat cggggaggta cagggtgatt tgtccatgct
    16861 ctttgacaag caatatatcc tgacagaaaa cgtcgtggta gaaacccgtt accgcaccga
    16921 aaccgataca tggacggacg cggagggcaa cacccataca gacacctacc aagtgccgta
    16981 tgactattac atctgctacg tcacgctgga aaacttcgac ctgtcccatg tgccagtcta
    17041 tatcatggac gaggaaacgc taagtttcta ttccctgtat atgtccacgc taggaaaccg
    17101 gcctgatctg ttttccggca gccagtaccc caacgcctcc accctgacgc cgccgaccta
    17161 ctacgacgta cccccggagg ctttggagga tgccaccttt gccgccatgc tcaaagaggc
    17221 ggaaaaatat atcggttatc cctatgtgtg gggcggcagc tctcccagca ccagctttga
    17281 ttgcagcggc ttcatttcgt gggtaatcaa tcatagtgga tggaacgtgg gacggctggg
    17341 ggcgcagggc ctatataaca tctgcacccc tgtttccagt tcgcaggcca agcccggcga
    17401 ccttgtattt ttcaagggaa cctacgacac ccccggcgtg tcccacgtcg gcctgtatgt
    17461 gggcaacaat gtgatgctgc attgcgggaa cccgatttcc tacacgaacc ttacttcaaa
    17521 ctactggcaa tcccattttt acagttacgg gcgtttacct taacgccaga aaggagcttt
    17581 caattatggc aaccaaactt gaccgcatcg aaaaggacat ccagaaaacc aagtctaaga
    17641 tcgcggagtt tcagaaacag cttcgggagc tggaaacgca gaaaaccgaa caggagaatt
    17701 tgcagatcat ccagcttgtg cgcggcatga acatgaagcc ggaggaattt gccgcgttct
    17761 tacgcagcgg cgcaatgcag gccgcccctg ccgcaacccc ttaccatgaa caggaggaca
    17821 atgcccatga agaataagac cagacggatt tcccgtattc tccccgcact tctggccgtg
    17881 atgctctgcg tcacggcctt tcccgttacc gcccttgcag gcggcaatga cccggcaccg
    17941 gccccgctgc cggaggctac aaccgaaccc gctacgggcg gtttggagcc tgaaacagac
    18001 gaaaccggca ccgccacgga cgggacagaa accgaggccg aaaaagatac cggccctctg
    18061 accggcaagg acatcaccga cctgttttcc gcgctgttcg gttccaaggt gagcattgcc
    18121 gccacggacg acggcatcca gatcaccacc ggcaccgaca aaaaagaacc tgaacagacc
    18181 ggcaccgtca ccaccaacgg cggcaggctg aatgtccgca ccggcgcggg gcttgacaag
    18241 accgcgttca cccagcttcc caatgggacg acggttgagg tagtcggcac agacggcgac
    18301 tggattaaaa ttctcttgcc ggagcgcatc ggctatgtcc attccgacta tatgaccgtc
    18361 agcgaaaagg aagtagcggc taccggcgag ggcggttttt ccctctccat tgacccggag
    18421 gaaattgctt ctctgctgga actgttcaat ggcggcgggg ccagcgccgc cctgtccccg
    18481 gacgggaacc tgtcactgat tgacgacatc ggcagcgccg cgaaaaatgg caagcagttt
    18541 atcaccgtgg aaaccaaaaa cggcaatgtg ttttatctga ttattgaccg ggacgacgag
    18601 ggaaaagaaa ctgtccattt cttaaatcag gtggatgaag ccgatctgct gacgctgatg
    18661 gaggacgggg aaaccgtcac ccccgctgct gtctgctcct gtaccacgaa atgcaaggcc
    18721 ggggctgtga acacgaattg cccggtatgt aaaaccaacc tgaccgagtg cagcggcccg
    18781 gagccgcagg aaccccagcc ggaggaaccc gaagccccgg aggaagaacc caagggcggc
    18841 gcgggcggcc tgatcgtgtt cctgctggtg gcgctggcgg gcggcggtgc ggccctttac
    18901 tatttcaagt tcaaaaagcc caaggctgaa accaccggca gcgacgattt gggcgagtat
    18961 gattttgggg aggacgagga tttggaggac gaggaacccg aaaccgagtt tgacccggaa
    19021 gctgattttg tgagtgaaca ggaggacgag gaatgacata taaacttgta atcgcggaaa
    19081 aacccagcgt ggcccgcagc attgcgggcg tgatcggggc cacggagaaa cacgacggct
    19141 atttggaggg gaacggctat ctggtgagct ggtgcatcgg acatctggtg tcctttgccg
    19201 acgccggacg ctatgacgag cgtttcaaaa aatggcgcta tgaggatttg cccattctcc
    19261 cggaaacatg gcagtacatc gtccccgacg acaagaaaca gcagtttgac gttctctgtt
    19321 cgctgatgca gcgccccgac gtgaccggcc ttgtgtgcgc caccgacgcc gggcgcgagg
    19381 gggaattgat tttccgtttc gtctaccaga tggcgggatg ccaaaagccc tttgagcgcc
    19441 tttggatttc ctctatggag gacagtgcta ttaaggacgg ctttgcccac ctgaaaccgg
    19501 gcggcgacta tgatccgctg tatcaatcgg ccctgtgccg ggccaaggcc gactggctga
    19561 tcggcattaa cgccacccgg cttttttccg tcctgtacca caagaacctg actgtgggcc
    19621 gcgtccagac gccgacctta aaaatgctgg tggacaggga cgccaagatt accgatttca
    19681 agaaagagaa atatcacatc gtccatatca cggcgggcgg cgcggacgcg gtaagcagcc
    19741 gcttttccga cgctgccgag gcaaacaccg tcaaggcggc ttgtgcaaaa gcgcaggccg
    19801 tttgcgtttc cgtgacgcgg gagaaaaaga cggaacagcc gcccaagctc tatgacctta
    19861 ccaccttgca gcgggaggct aaccggcttt tcggctatac cgccaagcag acccttgact
    19921 atgcccaggc actgtatcta aagaagctgc tgacctatcc gagaacggat agtcaatatc
    19981 ttaccgacga tatgctgccc gccgtggaaa gccttgtgtc cggcctgtgg gagctggtgc
    20041 cgtttgccaa gggcctgaac ctttccccgc agtttgaccg catcctgaac agcaaaaagg
    20101 tgtccgacca ccacgccatt atccccaccg ctgaatttgt gaagcagggc tttggcactc
    20161 tggccgagag tgaaaaaaag ctgctgtccc tgatctgctg caagctctta tgtgccgtgg
    20221 ccgggccgca tatctatgag gccgtcaccg ccacatttac ctgtgccggg caggagttta
    20281 ccgccaaggg caagaccgtc ctttcccccg gctggaaaga gattgaacgc cggttccgtt
    20341 cttccttaaa gaccgacgcc gacgaggacg ccgaggcggt gcgggagctg ccggagcttt
    20401 gcgagggcca gacctttgca gacgtggccg cctctgtcac cgagcatttc accacgccgc
    20461 ccaagcccta caccgaagat acgcttttgt ccgctatgga acgggccggg gccgaggata
    20521 tgccggagga tgccgagcgc aagggattag gcacaccggc cacccgcgcc gccattctgg
    20581 aaaagctggt gcagatgggc tttgtccaga gaaaaggcaa gcagctcatt cccaccaagg
    20641 acggcatcaa tctggctgtg gtgctgccgg aggcgcttac ttctccccag cttactgccg
    20701 aatgggaaaa ccggctgacc gagatcgcca aggggcaggc cgatcctgac gaatttatgg
    20761 acggcatcga gacgcaggcg cgggagctgg tgcagaccta ttcctgtatc agtgaggaaa
    20821 agcagaaatt gtttcaggcc gagcgtgttc ctatcgggac gtgtccccgc tgtggtgagg
    20881 ctgtctatga gggcaagaaa aactactact gcggaaaccg ggcctgccag ttcgtcatgt
    20941 ggaagaatga ccgatttttt gaggaacgca agaaagcgtt caccccgaag atcgccgctg
    21001 cgctgctcaa aaccggcaaa gtcaaggtaa agggcctcta ttccgtaaag accggcaaaa
    21061 cctacgacgg gacggtgctt ttggctgata ccgggggcaa gtacgtcaac taccgcattg
    21121 aacacaggag ctaaaggaca gtaacacagc aagcataggg agtgtggcta cactcccggt
    21181 aaaatccccc ggccgcgtcc ctgccgggcc ggggattttt gcttgttggg gaagtcccca
    21241 accccctatt ttttgaaaca gaacagagaa aggagtgacc gcgtgaacga agtattttct
    21301 atccaagtcc ataaccggca gtttttggaa cagggcaaac aaggcgtctg gctggatttg
    21361 cccaccacaa ccgaaacgct gcaagccgcc ctgcgggatg ttggcattac tgccgacaat
    21421 ccgcaggatt tttttatcaa cgggtattcc tgcccggagg acagacacct tgccattccc
    21481 tatgacatgg tgctggccgc cgacgtggac gagctgaatt tccttgccgc ccggctgggc
    21541 cagcttgacg ccgccgagat cgcagagctg aacgccgccc tgcaaaatcc ccgtggcggc
    21601 tttgagaata tcggccagat catcgactac accgaaaatg tggattacta tgtgcatctt
    21661 cccgacgtta ggggggatgg acagttgggc gactactacc tgaaccgttc cggcatggtg
    21721 gatatgccgg aggaatggaa agccgggatt gatacggcgc agtttggacg ccacattgcc
    21781 cagcaggaac aaggcgtttt tacccagtac ggctatctgg tgagaagcgg cgacgaatgg
    21841 cagcgcgtcc atgagggcca gccggtgccg gaggaatacc gggtgatgca ctacccccag
    21901 ccggaagctg accgggagga accgcaggcc cagcagccgg aggcatccga aagccccgac
    21961 aaagcgccgc ccccggttat cccgatcatg ctggacggca tgaacagcaa agaacgcatg
    22021 aaagtgatta ctgaccgtct ggaaaccggc gtacaggagc tttttgaaag tagccgctat
    22081 atggcttact tatccgctat gtccaagatg cacgattaca gtctgaacaa tacgctgctg
    22141 atcgccatgc agggcggttc gattgtggcg ggctataaca ggtggcagga tgaatttgac
    22201 cgccatgtga agcggggcga gaaaggcatt aagatactgg ccccggcccc gttcaaggtt
    22261 aagcagcaga tggagaaaat cgacccggcc acccagcagc ccgtgatcgg cgcggacggc
    22321 aagcccgtga ccgaagaaca ggaagtgacg gttccggcct ttaaggtggt gacagtcttt
    22381 gacatcagcc agaccgaggg caaggatttt cccatgccct atatcagtga acttaccggc
    22441 gacgtgcagc agtatcagga tttctttgcc gcgctggaac ggacatcacc ctatcccata
    22501 agcattgagc cgatttcaga cggggccaag ggccgctgct tctacgcaaa acagcgcatt
    22561 gccatcaacg agggcatgag cgaattgcag accctaaaga ccgccatcca tgagattgcc
    22621 catgccaagc tccacgccat tgaccgggag gcccccgccg aggaacaggc ggcccggcca
    22681 gaccgcagca cccgcgaggt acaggccgaa agcgtggcct ataccgtctg ccagtattac
    22741 gggctggaca cgtcggacta ttcttttggc tatgtggccg attggagcag cggcagggag
    22801 ttatccgagc tgaaagcctc ccttgaaacc atccgggcca cggccagcga attgataacc
    22861 gagatcgacg gccattttgc ccagcttcag caggagcgcg aggcccggca ggaacaggag
    22921 gccgtcacgg ttgacgttac cccggaggca gcggccccgg cgcaggccga ggaagaacag
    22981 gccccggccc ttgatccggc agccgagccg cttgtgactg tgctatggag cgaaagcccc
    23041 cagctaaagg acggccagca aatgccgttg catgaggccg acgcgctgtt tgcccgtctg
    23101 gatgcagcat atccggcaga ggcaggctat gacaaaaccg cgttccgcat tgactgtatc
    23161 tttcagggcg agccggacag ctacgagggg cggcaggatt ttggagatag ggacggttcc
    23221 ctgatcgaac atatccagag ctaccaccaa ttttacgcgc aggacgaaaa ctggaaaaag
    23281 catgtaatcg ctacaaccgg catagagggc tgggagcagg acaaggccca tcgggatatg
    23341 ctgttaggcg agtttgtccc ctatatgaag ctgcattgca gccttgccca aatggaacag
    23401 acggcgcagg gccgcttaca gtccggggaa accctgacgc cggaggaaac ggcctatttg
    23461 ggggcgcttc tctcttatgt gcaggagtgc cgccccatgc tcaatcaggg ccagtatcag
    23521 ttgccggagc cgccgaaact ggctgacttc gaccagagct tgcaggacta caaagcacag
    23581 gtacaggccg agatcgcgca ggaggccgcc gcagccggta tgaccgtgga ggaatacgcg
    23641 gcggctggct atgaagcacc ccaacaggac accttttcca tctaccagct tcgaggcggg
    23701 gaggaaaccc gcgactatcg gtttgagcca tacgagcgtt tacaggccgc agggctggcc
    23761 gtggacagtg ccaactatga ccttgtttat acggcaccgc tggataaaag cacgtctttg
    23821 gaggatattt accgcacatt caacatcgac cacccggcag attttagggg acacagcctt
    23881 tccgtttccg acgtggtggt gctgcaccag aatggacagg acaccgccca ctatgtagac
    23941 agcttcggct atatggaagt gccggagttt ttgcgggaaa atcccctgcg taccgccgaa
    24001 ctgtctacgg agcagaacga aaacatgatt gacggcatta tcaacaatac gccgcccgaa
    24061 ccgcagccgg aggaacccga acagctatct tttgaggatg tggcagttgc ttccggccag
    24121 acacaggagg aacccccgga ggctgcgccc ccgcaaacgg ctgtccgcta ttatcccatc
    24181 aatgagggag ccgcccgccg cgccaaggaa gccatgagct tttctgacta ccggcccggc
    24241 agcgccacgg cggaataccg gcactatgtc gatcaggccg tggaaattgc ggagcgccag
    24301 aaaaagcgtg tggagccaga gtatcacgaa aagatagaca gcctccttga cacctacgcc
    24361 cgcaagctgg ccgagaacat gaataagggc tatgagatta ccgcccgcgt cccctctgtc
    24421 ctgattgccg ggccgtccaa tttcccggta cgggcgaaag aaaagcagaa cgccgccagt
    24481 gaccgcaaca tggaggaatt tcagtacatt cagggcttgc ttgacaaaat ccgcagcacc
    24541 ggcatgggtg gtatcagcgc cgacgatccg caggccgttt ccaagctgga aaagaaactg
    24601 gaaaagctgg aagcgtcgca ggagttgatg aaggccgtca atgcctacta ccgcaagcat
    24661 gggacgctgg acggatgccc ccatctgacc gaaagaggca ttgaaaacct gaaagcggat
    24721 atggcgtcgg gctggcacta tgagaaaaaa ccgttccagt cctggcagct ctccaacaat
    24781 aacgccgaga tacgccggtt aaagggccgc attgaggaat tgacccgcca aaaggaggcg
    24841 gcctatgtgg gatgggagtt tgacggcggc actgtggaga tcaaccgggt ggccaaccgc
    24901 ctgcaaatct tctttgaggg gaagccggat gctgctgtac gcgacgagct gaaaagcaac
    24961 ggtttccgct ggtcgcccaa ggccggggca tggcagcggc agttaaacga caccacaatc
    25021 cgcgtcgctg accgtatcaa gtgcatccag cccctttccg gcgaaaagcc cagcgcctta
    25081 cagcttgcgg cccgcaggga gaaagaaaag ccctctatcc gggcgcagct caacgcggcc
    25141 aagaccgaac aggccaagca gccaccggcg cgggaaaaat caaagggatt ggaggtagag
    25201 taaatgcaga agtttgaaaa tgtggacgtt ctggccgcgc tggaacagat catgcggcag
    25261 aacaccgcgt tttatcaaag tgactttggc attgacaaag acattatcag gcgggatgcc
    25321 gccagcgatc aggccgcaga caagaccctg ctctggatgt cccggccctc cgggacgtac
    25381 tgtttccggg aacgtgatgt tttcctgaaa gacacccgac agtacaacac atggaaattt
    25441 tacggggagc agacccgtga caagatactg gcctacgcgg tggagctgac cggcacacag
    25501 ggcggcacta tccggggcaa tctctatgag ctggactatc cgcagcattt ccggcacgtc
    25561 attgaaaccg cccagccggt gagtgtgaac cggctctttt atgagcatgg cacccgtgac
    25621 atcccggaga gccagcgttt tgacggaagc cctgaccggg tattgggaaa ctttttgtac
    25681 tatgaggccc agccccatga cccggctgtg ctgcaggagg cgctacggac ggagcagcac
    25741 agccgggggc gggccgtccc cggcgatttc aaagtccacg tcaccgcttt gcatgacagc
    25801 cggattgaaa ccgaggcccg gcgtattgtg gacagcctga aagatctgtc cgcgcctaac
    25861 agccccaata aaacgcattt catggtggag atctccccgc actttgcggc gattgcttcc
    25921 accaaggata atgaccggct ctttgccatg ctgccctata aatccctgtg ttttaccggg
    25981 atgaaaggca ggcatggcgt gtatgccgtg attaaccggg acgagcgccg ggatgtgagt
    26041 attcgcaggc cccggccctc tatccgcaat cagctatccg acagcaaaca ggccgccagc
    26101 ccaaaaaagg cggcggccag aaccaaaaag aatgaattgg aggtataagg aatgatgcac
    26161 tttaccgtgg aggaagaaaa cctgatctgt atgtaccaca atgcagaccg gcgcagaacg
    26221 ataaacaggc tcaccgccgt catgccggtt atggatgggg atatgcgggc gcaggcccag
    26281 cagacccttt ccaagctgga acgcatgacg gatgccgact atgacggcca gaagttccac
    26341 ttcacagacg aataacgaac agcagacgcc gggcgcggcg gccatgtggt cgcttgcgtc
    26401 cggcgtcttt tctttttgcc tattcctacg catttagaac ccggtattgt cggggatagg
    26461 atgaagtatt cgcttgcccc tgcaaacatg cgggagaagc cttgcagcgg gcggttttca
    26521 atcccttaaa tgcgtaggtt gcagctataa ttagacccct gaaacagata tagccgcgca
    26581 gcgggtgtat ctgtttcagg ggtgtgcagg tgatagccgc aaagcggcgt aagagggtgc
    26641 agccctcttg ccgtctatct ctgttccagc gggaagctcg gcaatgcttc cagcagtttc
    26701 agggaaatgc gctgccgtat atcttcgtca atgactttct tctttttgcc gtttgcctgt
    26761 ctgatctcca ccgtggaaag ctcgttgatg tagtcagcat aatgggccaa cactttttcc
    26821 gtggcccacc tctcaccggc aacggcggct ttgatcgttt catagtccag cagcttttct
    26881 tgtccgtcca tcctgatacc cccgtcagac tgattttttc tatgatacca tattcggaag
    26941 catggaacca gagaaaacgg cgcttttctg ctggtaaatt ctacgcattt agaacagcac
    27001 ttcggcgggg ataggataaa acacccacat accccgctga ccatgccgga aaagccttga
    27061 aatataaggg cttttacagc tctaagtgcg taggtttcca gtatcttttc cgctggcgct
    27121 cggcctcttt gcggcggcgc atccgtttgg agcaggccgc gcagtatttg gcccggttag
    27181 acttcggcgt gaacgtggcc ccgcactcac agcagcgttt cacttcgtcc cggtctttgg
    27241 tgatctcggc atacagggcc gcatccagcg ggaggacagc ggccttaaac cagcggcaca
    27301 gaagcgaata ggaaatgctc tgtacgcaaa tacattcttc gccgtcgtcc agcaacaggc
    27361 agttgccgtc acaatagttg cagcactcat gcgtcagctt ccgggccttg cgatattgag
    27421 ggtaattcat tcgtggaata ctcatagctc ttgcgtccat tcctttcccg gcgtcatacg
    27481 caaaatgccg tccacattct gcttgatgat gccgtattcc tgcatctgct ttttcgcttc
    27541 gccgtacttc tcatacagcc gttccttttc ctcggccaat cgcttgtact cggctttcag
    27601 ggcgtacaga tcggggagct ttttcacacc ctgtcccttt agggcctttg ccgcagcttc
    27661 atacaaaatc aacgtgcttt catgctcacg ccggaatttt gccttatccg ctgcattgcg
    27721 gtattccata gccacgggcc agagctgctt gtaggtagaa atattcttta tcagcaccgc
    27781 catatccgca agccggtgtt ccatagcttt gagggtagcg gcggcctcgt cgctggcggc
    27841 ggtgatctcg gccactttgc tttccaaatc cgggtaggtg tctatcccat gttcagtcag
    27901 gaaattcaaa gtctttgcgg cctgcttcaa attgtggagc ttcgcccacc tctcatagcc
    27961 tgccgactgc tgggccttaa tgttgttttc cagatcaatc cgcaaagaaa tccccttgcg
    28021 ctctttgggc gctctggtga taacgcggcc tttgatacgc tctttgatgg cttcctcggt
    28081 gtaggcttcc cccaatgtct tgcagcgggt aaagcgttcc tgtccgggag cgcggaaaga
    28141 aataaacttt cccggcttga tctcatagcc ctgcgcctgc atgagccgca ggaaatcatc
    28201 aaaatctttt gcctgcggga tagcggcgtc aatggctgct ttgagcttcg ctttccagct
    28261 cgtccctttc cgctgggcgt cccactcggc atagctcttg cccttactct ggccgggctt
    28321 caccacggac aatccgtttt ccttgcacag cctgtcgctg gtgcgccgga tataggcata
    28381 gctctgtttg ttggaaatgt acttgcgctg atttaccata tccacggcac agaaaataat
    28441 gtgattgtgg acgtggccct tatcaatatg ggtagtcagc acatagggat atttcccttg
    28501 cagcacttcc tcggcaagct gcttcccgat ctcatgggcc tgttccggcg tggcttcgcc
    28561 gggggcaaag gattgtatca ggtggtggcc cagattattg cccttttggt atgcctgcgc
    28621 cagcagcatt tcaaattcaa tgtccgctgt ttcataagag caggcaaagg aggccaccag
    28681 cagcttgccg tcagttttgt ccgggttctc gatgtagtca agggcttttt tcagcgttga
    28741 cttgatagga tgtatctttg taactgccat atctgcgcca gcgccccctt tatatcctcg
    28801 atgtcctgtg cgtacaccgg ccccataccg ttcagcctct ttgcaatctg attgacgttg
    28861 acgccgatct tctgtatctc ggcggtctgc gcccggatgt cgctggtgtc catgttgacg
    28921 atatagccgt caatgagcat cttccgggcg taggctgaaa agttcttcgt atgcagcagc
    28981 gccattttct gttcaatcat agcccgttcc tccggcgtca cagggacgcg cagcacgatt
    29041 tttcgctttc tgtttgccat gcttcaccgt cctttccatt caggggtttg gggatttccc
    29101 caacaagcaa aacggacgcg gcccggcagg gacggggcag gccgtttttc cgggagtggc
    29161 tacactccct atgcttgcta cgttactgtt tctccactaa caagacgttt ccaaagcccc
    29221 aaactacccg caaaatagcc ctctggaaag aaaaaagtgc aaaaaaatag aagctacgct
    29281 gtgcagctcc cgctaaaagt aaaacagagg atgccagtta cacatcctcc gtttcattct
    29341 tcttcggctt ttcaattagt atggcgctgt caatcgcgcc ctcgatgatc ggcaggtatt
    29401 cttcggggca gagtttcagc ttgtggctga ccctttgccg cagttcgctt tcctcccgca
    29461 tgatctccgg gttaaagtag cgttccacag gaagcccgca ggctttaata agctgtatga
    29521 caaccggcag actgggaatg gccccgtcgt tttcgatgtt ggcaagatag cgccagtcaa
    29581 tatttatttt ctctgccatt gctttgcgcg aaacgcgctt tgcttgccgg gccgctttca
    29641 catccgcgcc gaaagtttca aagccgggac aatcttcaat attcgccata tcgcacctcc
    29701 cacttacatt gtatatttca tactctgccc gtggaatgtt ggtatatgca cctttcttaa
    29761 agtttatatt tcacattttg tttcttttat tcttcgagca aaagaattat aatgtaccct
    29821 gtggaggtgt actatggact acatgacgtt gaaagaggca ggcgagaaat ggggtgtgcc
    29881 cccacgatgg ataaactact attgtgctgc gggacgcatc cccggcgctg taaagatggc
    29941 gacgatctgg ctcattccca aaactgcgga aaagccgatt gacaggcgta caaagaaagg
    30001 aaaggcggtt actgaatagt ataatgaaac cggcgcattt tggatatggt tttgaagaga
    30061 gagaaagcat ggtattttgt cacggaaaaa ctataagatg atagatggca ggctgctcca
    30121 gaccgataaa aagtattctc atctgaaaca gaaagaaaag atttacacat ggatgtatga
    30181 agaagcgaaa ctttatcatg acagaaatgg aaaatgcccg aaacaaagac gaggaagctg
    30241 ctgctgtatc agccgtatat ggtagaattg aaaaggccgg catctggatt ctttatggtg
    30301 aagtctccag acattggata actgctggtc taatgcgcct ttgagtatgg ggtgattgat
    30361 tttaataccg gggaggtacg gctttttgag gaaaatattc tgggatttac atttggcgat
    30421 tattacattt cctgtaactg caaggacacg gatgatgaag atatgaggaa tatagacttg
    30481 ctgattttct actgtcttga acggtattag gactgaatct gctgcgcgga gtgcggcaag
    30541 agcgaccgga gggagtacac acaggccctg cctgtgtgaa aagccatacc gcaaggtgca
    30601 ttcgcaaaaa agaggccgcg cattttgctg aggcttgggg tgtccccaac aagcaaaacg
    30661 agaacgccgc aggcgggcaa gtggtggatc agaatatccg taaaggatat tctgatccat
    30721 tgcttgccag tattatcggg atgttttgac aaccgctgta atcgaaacat cccaaacatt
    30781 aagaaggtgt taattattat tatcttgatt taattcgttt aaaccaataa tatagataca
    30841 tacaccatga aaagaagcga gtatcccaat gacggatgat taaaatccga aaagaaatcg
    30901 ctttgaaact acagggaaac tacagactgt tatgttatct tcttaaaatg gagggatttt
    30961 tatgtcgata cgaattctac ttgtcgagga tgatgatcat atctgtaata cagtaagggc
    31021 gtttctggct gaggcaggat atcaggtgga tgcctgcaca gatggaaatg aggcatacac
    31081 caagttttac gaaaacactt atcaactggt tattcttgat attatgctgc ccggtatgaa
    31141 cgggcatgaa cttttgcgtg aatttcgtgc gaaaaatgat actcccattc tgatgatgac
    31201 agccctgtcg gatgacgaaa accaaatccg ggcgtttgat ctagaggcag acgactatgt
    31261 aacaaagcca ttcaagatgc agattttact aaagcgggtg gaagccctgc tgcggcgcag
    31321 cggtgcgctg gcaaaggaaa tccgtgtcgg caggctgaca cttctgccgg aggatttcac
    31381 ggtactttgt gacggtacag agctgcccct gacacgaaaa gaatttgaaa tccttttgct
    31441 gctggtgcag aacaaaggca gaaccttaac gcatgaaatc attttgtcac gtatatgggg
    31501 atatgacttt gagagtgatg gcagcacagt ccacactcat atcaaaaatt tgcgggccaa
    31561 gctgccggaa aatatcatca aaaccattcg cggtgtagga taccgattgg aggaatcata
    31621 ataatggaaa gaaaagggat tttcattaag gttttttcct atacgatcat tgtcctgcta
    31681 ctgcttgtcg gtgtaacagc aacactgttt gcacagcaat ttgtgtctta tttcagagtg
    31741 atggaattac agcaaacagt aaaatcctat cagccattgg tggaactgat tcagaatagc
    31801 gataggcttg atattcaaga ggtggcaggg ctgtttcact acaataacca atcctttgag
    31861 ttttatattg aagataaaga gggaagcgta ctctatgcca caccaaatgc caatacatca
    31921 aatagtttta gacccgactt tctttatgtg gtacatagag atgataatat ttcgattgtt
    31981 gctcaaagca aggcaggtgt gggattgctt tatcaagggc tgacaattcg gggaattgtt
    32041 atgattgcga taatggttgt attcagcctt ttatgcgcgt atatctttgc gcggcaaatg
    32101 acaacgccga tcaaagcctt agcggacagt gcgaataaaa tggcaaacct gaaagatgta
    32161 ccgccgccgc tggagcgaaa ggatgagctt ggcgcactgg ctcatgatat gcattccatg
    32221 tatgtcaggc tgaaagaaac catcgcaagg ctggaggatg aaatcgcaag ggaacatgag
    32281 ttggaggaaa cacagcgata tttctttgcg gcagcttctc atgagctaaa aacgcccatc
    32341 gcggctacaa gcgttctgtt ggagggaatg cttgaaaata tcggtgacta caaagatcat
    32401 tctaagtatc tgcgcgaatg catcaaaatg atggataggc agggcaaaat catttccgaa
    32461 atactggagc ttgtcagcct gaatgatggg agaatcgtac ccatagctga accgttggac
    32521 atagggcgca cggttgccga gttgctgccc gattttcaaa ccttggcaga ggcaaacaac
    32581 cagcggttcg tcacagatat tccagccggg caaattgtcc tgtccgatcc gaggctgctc
    32641 caaaaggcac tatccaatgt catattgaat gcagttcaga acacgccgca gggaggcgag
    32701 gtacggatat ggagtgagcc tggtgctgaa aaatgccgcc tttttgtttt gaacatgggc
    32761 gttcacattg atgatactgc gcttccaagg ctgttcaccc cattctatcg cattgatcag
    32821 gcgcgaagca gaaaaagtgg gcgaaacggt ttaggacttg ccatcgtaca aaaaacgctg
    32881 gatgccatga gcctccagta tgcgctggaa aacacctcgg atggcgtttt gttctggctg
    32941 gatttaccgc tcacatcaac attgtaaata tttaaaactt aaatgatttt gaccgacagg
    33001 tataaccctg ccggtctttt tttgctgcta caggaaaact acagactggc tacagggaaa
    33061 gtacagatgc gcttgccata ataacaatcg taccagccac aaatcgtagt tttattgcaa
    33121 aggaggcatt cgatcaaatg gaaaaaagca actatcattc caatgcggat catcacaaac
    33181 ggcatatgaa acaatccgtg gaaaagcggg cttttctatg tgcgttcatt atttcgttca
    33241 cagtctgcac gctgtttttg gggcggagac tggcttccgt attggaggca acacagatac
    33301 cgcccatccc tgcaactcat acaggcagca gcactgacgt agtggagaat ttggaggaaa
    33361 acgctcttgc caccgccaaa gaacagggag atgaacagga atggagcctg attttagtga
    33421 acaggcagaa ccccatcccc gcacagtacg atgtggagct tgagcaacta tcaaatggtg
    33481 agcggataga tattcggatt tctccctatc ttcaagattt gtttgatgcc gcaagaactg
    33541 atggagttta cccgattgtc gcatccggat accgaacaac agaaaaacag caagaaatta
    33601 tggatgaaaa aattgccgaa tataaggcga aaggctacac ctctgcacag gctaaagcgg
    33661 aagcagaaac ttgggtggcc gtgccgggaa cgagcgagca tcagcttggt cttgctgtgg
    33721 atatcaatgc ggacggaatt cattcaacag gcaacgaggt ttatagatgg ctggatgaaa
    33781 acagctatcg ttttggtttt attcgtcgct acccaccaga caagacagag ataaccggtg
    33841 tgagcaacga gccgtggcat taccgatatg ttggcatcga agctgccaca gagatgtaca
    33901 accaaggggt ttgccttgag gaatatttaa aaccagaaaa atgagaaaag gatgttatgg
    33961 acagaaaaag attgacacag cgtttcccgt tcctgcttcc aatgagacgg gcgcagagaa
    34021 aaatgtgctt ttatgcggga atgagatttg acggctgtcg ctatgcgcag acgatagggg
    34081 aaaaatcgct ttcccatttg ctctttgaaa cggattgcgc attatacaac cacaataccg
    34141 gatttgacat gatataccag gaaaacaagg tgttcaactt aaagctggca gcaaagacct
    34201 taaacggctt attgataaga ccgggggaaa ccttttcttt ctggtggctg gtacgccatg
    34261 cggacaaaga taccccctat aaagacggcc ttacggtgac caatggtaaa ctcaccacca
    34321 tgtcgggcgg cggtatgtgc cagatgagca atttactatt ttggatgttc ctgcatacgc
    34381 cattgacaat tatccagcgc agaggtcacg aagtaaagga gtttccagag ccaaacagtg
    34441 acgagattaa aggggtggat gcaaccattt cagagggctg gattgattta aaagtgcgaa
    34501 acgataccga ctgcacctac caaatatgag tgaccttaga tgatgagaaa atcatcggtc
    34561 aggtgtccgc cgacaaagag ccccaagcat tatacaaaat tacaaatggc agtatccagt
    34621 atgtccgtga aagtggcggg atttatgaat atgcccaggt taaacggatg caagttgcct
    34681 taggtaccgg ggaaataata gattgcaagc tgctttatac aaacaaatgc aaaatctgtt
    34741 atcccctccc ggaaagtgtg gatatt
//
LOCUS       DAGCYL010000010        34469 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.010, whole genome shotgun sequence.
ACCESSION   DAGCYL010000010 DAGCYL010000000
VERSION     DAGCYL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.010"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            165..557
                     /locus_tag="MB216_000356"
     CDS             165..557
                     /locus_tag="MB216_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4525217.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            585..1925
                     /gene="glnA"
                     /locus_tag="MB216_000357"
     CDS             585..1925
                     /gene="glnA"
                     /locus_tag="MB216_000357"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBT4525218.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3118..4728
                     /locus_tag="MB216_000358"
     CDS             3118..4728
                     /locus_tag="MB216_000358"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBT4525219.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5020..5889
                     /locus_tag="MB216_000359"
     CDS             5020..5889
                     /locus_tag="MB216_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBT4525220.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6054..7325
                     /locus_tag="MB216_000360"
     CDS             6054..7325
                     /locus_tag="MB216_000360"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBT4525221.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7322..8617
                     /gene="rho"
                     /locus_tag="MB216_000361"
     CDS             7322..8617
                     /gene="rho"
                     /locus_tag="MB216_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBT4525222.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8716..8979
                     /locus_tag="MB216_000362"
     CDS             8716..8979
                     /locus_tag="MB216_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBT4525223.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9424..9873
                     /locus_tag="MB216_000363"
     CDS             9424..9873
                     /locus_tag="MB216_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4525224.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9930..11348
                     /locus_tag="MB216_000364"
     CDS             9930..11348
                     /locus_tag="MB216_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4525225.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11348..12208
                     /locus_tag="MB216_000365"
     CDS             11348..12208
                     /locus_tag="MB216_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBT4525226.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12382..13155
                     /locus_tag="MB216_000366"
     CDS             12382..13155
                     /locus_tag="MB216_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4525227.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13302..13667
                     /locus_tag="MB216_000367"
     CDS             13302..13667
                     /locus_tag="MB216_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBT4525228.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13815..15023)
                     /locus_tag="MB216_000368"
     CDS             complement(13815..15023)
                     /locus_tag="MB216_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4525229.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15042..15320)
                     /locus_tag="MB216_000369"
     CDS             complement(15042..15320)
                     /locus_tag="MB216_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525230.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15474..16169
                     /locus_tag="MB216_000370"
     CDS             15474..16169
                     /locus_tag="MB216_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBT4525231.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16611..17408)
                     /locus_tag="MB216_000371"
     CDS             complement(16611..17408)
                     /locus_tag="MB216_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBT4525232.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17405..18589)
                     /locus_tag="MB216_000372"
     CDS             complement(17405..18589)
                     /locus_tag="MB216_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBT4525233.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18614..19861)
                     /locus_tag="MB216_000373"
     CDS             complement(18614..19861)
                     /locus_tag="MB216_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBT4525234.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20076..21821
                     /gene="dnaX"
                     /locus_tag="MB216_000374"
     CDS             20076..21821
                     /gene="dnaX"
                     /locus_tag="MB216_000374"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBT4525235.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21841..22155
                     /locus_tag="MB216_000375"
     CDS             21841..22155
                     /locus_tag="MB216_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBT4525236.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22180..22776
                     /gene="recR"
                     /locus_tag="MB216_000376"
     CDS             22180..22776
                     /gene="recR"
                     /locus_tag="MB216_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBT4525237.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22840..23484
                     /locus_tag="MB216_000377"
     CDS             22840..23484
                     /locus_tag="MB216_000377"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBT4525238.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23499..23828
                     /locus_tag="MB216_000378"
     CDS             23499..23828
                     /locus_tag="MB216_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBT4525239.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23842..24780
                     /gene="holB"
                     /locus_tag="MB216_000379"
     CDS             23842..24780
                     /gene="holB"
                     /locus_tag="MB216_000379"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBT4525240.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24816..25640
                     /locus_tag="MB216_000380"
     CDS             24816..25640
                     /locus_tag="MB216_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBT4525241.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25633..25980
                     /locus_tag="MB216_000381"
     CDS             25633..25980
                     /locus_tag="MB216_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBT4525242.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26049..26921
                     /gene="rsmI"
                     /locus_tag="MB216_000382"
     CDS             26049..26921
                     /gene="rsmI"
                     /locus_tag="MB216_000382"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBT4525243.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27030..28151
                     /gene="dinB"
                     /locus_tag="MB216_000383"
     CDS             27030..28151
                     /gene="dinB"
                     /locus_tag="MB216_000383"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBT4525244.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28205..28807)
                     /gene="nrdG"
                     /locus_tag="MB216_000384"
     CDS             complement(28205..28807)
                     /gene="nrdG"
                     /locus_tag="MB216_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBT4525245.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29122..31311)
                     /gene="nrdD"
                     /locus_tag="MB216_000385"
     CDS             complement(29122..31311)
                     /gene="nrdD"
                     /locus_tag="MB216_000385"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBT4525246.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31610..32404
                     /locus_tag="MB216_000386"
     CDS             31610..32404
                     /locus_tag="MB216_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4525247.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32394..34436
                     /locus_tag="MB216_000387"
     CDS             32394..34436
                     /locus_tag="MB216_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4525248.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11068 a         6117 c         7243 g        10041 t
ORIGIN      
        1 gtatctggta cgaaaatcaa ctagatacct tttttgtgtt cttatttttt tgttatttta
       61 taaaaaatag agatctcatg ttagaaaacc taacatatgc agttgacaat atttttttta
      121 attggtacac tttagccaac ttataaaacg aaaggggacg tctgatggga gaaaaggaat
      181 tgcgccgatc tatgtccgta tttccaattg gtacagttat gaagctgact gatttgtctg
      241 ctcggcaaat tcgctattat gaagaacaag atttagttca tccggaacga agcgaaggaa
      301 accgacgtat gtattcgctg aatgatattg atatgttgct tgagattaaa gactatcttt
      361 cagagggatt gaatatggct gggatcaaac gtgtatacga aatggcacag gcgaaaaaac
      421 aagaagcaaa aaacaagaag ccgttgacag atcatgacgt tcgacaaatt tttcgtgatg
      481 agattttggc gcaaggtggg ttaagcaaaa caggtcatta tcagtcgcaa ggattgcaaa
      541 gacaattctt tgactgaata aattaaaaaa ggaagaggga taccatggta aagaaacaga
      601 taacaggtga agaaatcaaa cgaatcattg aggaagaaaa cgtacgattt ttacgattga
      661 tgttcacaga catcttaggt acgatcaaaa atgtagaagt tccagtgagc cagatcgaca
      721 aagtattaga aaataaaatg atgtttgacg gttcttcgat tgaaggattt gttcgaattg
      781 aagaaagcga tatgtatttg tatccagatc tctccacttg gatgatcttc ccgtgggaaa
      841 gtgctcatgg aaaagttgct cgtttaattt gtgatatcta taatccggat gggacaccat
      901 ttgccggcga tcctcgcggg aatttgaaac gagcattgaa agatatgcgc gaccttggtt
      961 ttacctcttt caatttagga cctgaaccag aattcttctt atttaaatta gacgaagacg
     1021 gtgggatcac aacgaccttg aatgataaag gtggttattt tgattttgca ccaacagatt
     1081 taggtgaaaa ttgccgccgt gacatcgtgt tggaattaga aagtctaggc tttgaagtgg
     1141 aagcttcaca tcatgaagta gcgccaggac agcatgaaat tgactttaaa tatgcagatg
     1201 tggtagatgc ttgtgataat atccaaacct tcaagctcgt tgtcaaaacg attgcacgca
     1261 agcatggact tcacgcgact ttcatgccga aaccgttatt tgggatcaat ggttctggta
     1321 tgcattgcaa tatgtctttg ttcaatgatg aaggaaatgt tttctatgat aaagacggtg
     1381 aattggaact aagtgaaaca gcttatcatt tcttaggtgg tttgctgaaa catgctcgtg
     1441 cctacacagc cgtttgcaat ccaacagtca attcttataa acgtctcgtt ccaggttatg
     1501 aagcgcctgt ttatgtcgca tggagtggcc gtaatcgttc gccattagta cgtgtaccag
     1561 aatctcgcgg tttatccaca cgtttggaat tgcggtcagt tgatccatct gcaaacccat
     1621 acttggcaat ggcagtattg ttgcaagcag gactagacgg tatccgtaat cagatcacac
     1681 cgccagcagc tgttgaccgt aacatttatg taatggatga ggaagaaaga gaagctgcac
     1741 atatccagga cttaccttca actatccata atgctatcaa agaattgcgt aaggataatg
     1801 taatgattga cgcattagga caacatatct tctcaaattt tgttgaagca aaacgtctcg
     1861 aatgggcggc attcagacaa actgtttccg agtgggaaag agaacaatat ctagaacttt
     1921 attaaaacag gaataccgtg aattagaggc tgtgaaatca gcgttttgac ctgagtatta
     1981 gagaaactaa cggaaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg
     2041 tcagctgatt gaacaccgtt tagtggagag gttgtgaaaa agctgccctg caacaagtaa
     2101 taagaatagc caagcaaaaa cagcttttct tgtttttgaa ttggctgaac ttattaccgc
     2161 agatgcaagc ttttgaacac cgtttaccaa gaggttttga aaaagacgtt tagtttcgag
     2221 tattaagaaa aattttgtga aaatggcttt ctacattttt gcataaattt ggcttaatac
     2281 cgagaaactg gcttttgaat accgtttatc cagaggttgt gagcctacca tttaactccg
     2341 aatcacaaga aataatttca aaaacagctt ctcatgtttg atggatattg tgacttgtga
     2401 ccgaggagtt ggcaggagaa caccgtttat ttagaaataa ggaggcacga cttttgtcgc
     2461 atctccttat tttttactta tgtgattcgt taccttcaat gaatccctac aaggcacttt
     2521 tgttgaaaaa aaggtcatct ttgaaaaaat tagtggaatt gttttgtttt tgatattatt
     2581 taatagaaaa gatatgccaa aaatccgaga actgtggact acgtttttta cgtaaaggag
     2641 gtaaataagg gtgaaagaaa aagattgttt ctacgataag ctatataaac tgttgctgca
     2701 agtaggaaaa agtagttctt tgttaggtgg gatattatct atcataatgt ttgttttatt
     2761 tttttatcta ttatttcact taaaattttt cagtaacgat tttctgattt gcttaggttt
     2821 aacgttagct atgtttatat caagcttatt atgtgggtta aatttttttt gcactcataa
     2881 aggacttaag aaataatttc tcaaaggata tcgaaaaaga aaaaattgta agcggatagt
     2941 attcatttga gtacttgact ttcccttagt cagcttgtat tatacacctt gggcaccctt
     3001 ttagagggtc agaagtttac agaaatttcg gctccctatt cttattgaag aatagggagc
     3061 tttgtttatt gtctgagact tttcctgcac ataaattaaa aaggagtttt tgaacttatg
     3121 acaaaatata tttttgtgac cggcggtgtc gtttcatcaa tcggaaaagg gattgttgca
     3181 gcttctttag gacgcttatt gaaaaaccgc ggcttaaaag taactatcca aaaatttgat
     3241 ccatacatca acgtggatcc ggggacgatg agcccatatc agcacggaga agtattcgta
     3301 acagatgatg gtgctgaaac agatttggat ctgggccact acgaacgttt tattgacatc
     3361 aatttgaaca aatactccaa cgtgacgaca ggaaaaatct attctgaagt tttgcgtaaa
     3421 gaacgtaagg gcgaatactt gggagcaact gttcaagtta ttcctcatat tacaaatgag
     3481 atcaaagaaa aaatcatgcg agcagctaaa atgactgatt cagatgtgat cattacagaa
     3541 gtagggggaa cagtagggga tatagaatct ttgccattcc ttgaagcact tcgtcaaatg
     3601 aaagcagatg tcggtacaga taatgttatg tatatccata caacattgat tccttacttg
     3661 aaagctgctg gagaaatgaa aactaaacca acgcagcaca gtgtaaaaga gctacgtggt
     3721 ttaggtatcc aaccgaatat tttggtggta cgtacagagc aacctgtttc tcaaagcgtg
     3781 aaaaacaaat tggcacaatt ttgtgatgtc gaaccagaag cagttatcga gtcgccagat
     3841 gtagatacac tttactccat cccattagtt ttacaagcac aaggaatgga tcaaattgtt
     3901 tgcgaccatt taaaactaga tacaccagaa gcagacatga cagaatggaa agaattagaa
     3961 gaacgtgtct tgaacttgaa gaaaaaagta cggattgcgc ttgtcggtaa atatgtcgag
     4021 ttaccagatg catatatctc agtagttgaa gcattgaaac attctggttt cgcctataat
     4081 tcagacatcg agatcgattg gatcaaagca caagaattaa cgagagaaaa tgtagaagaa
     4141 cgtttgaaag acgcagatgg aatcttggtt cctggtggat ttggtgatcg cggagtagaa
     4201 gggaaaatcg aagcgattcg ttatgctcgc gaaaatgacg ttccgttttt aggtatttgt
     4261 cttggtatgc agatggcttg tgtcgaattt gcacgtaacg tcgttggatt agaagatgcg
     4321 tcatcagctg aaacgaatcc agatacagca aacaatatta ttgatttaat ggctgatcag
     4381 gaaaacatcg agaatttggg tggaacttta cgccttggat tatacccatg taaaatcaaa
     4441 aaaggaacga agacggctga agcatatgat ggagccgatg ttgtgcaaga acgtcaccgt
     4501 caccgctatg agttcaataa caaatatcgt caattgtttg aagaacaagg gctagtcttt
     4561 tcaggtgtct caccagataa ccgtttagta gaaatcgtag agctgccaga gaaaaaattc
     4621 tttgtgggat gccaattcca cccagaattg atttctcgtc caaatcgccc gcaaaaatta
     4681 atcaaagcat ttgtcggagc atcattagca gatcacgaaa caaaataatt tttgaacttg
     4741 atagaaagag aaaagtagca agagaagggg ataagcaatt ttcttgtcac ttttctcttt
     4801 ttctgatttg ttattatttg taggaaaaaa gatagaaagc aaaagacttt tttcgattaa
     4861 agcgctattg gatattttat tttttgcaaa aaacttcttg aaaattttca ttttcattga
     4921 ttttagtaga aaactaccga aatgtttggt aaaatagggt cgttggttaa gaattgtctt
     4981 aacaaattta gttttacaaa acttaggagg aattttatta tgccagtagt atcaggagct
     5041 gaatttttaa aagctgctcg taaaggcggt tacgctgtcg gcggattcaa cacaaacaac
     5101 ttggaatgga ctcaagcaat ccttgaagcg gctgaagcaa aacaagcacc agtacttatc
     5161 caaacatcaa tgggtgcagc taaatacatg ggtggttaca aaatctgtaa agatttaatc
     5221 actgatttag ttgaagcaat gaacatcact gttccagttg ctatccactt agaccatggt
     5281 gattacgaag ctgctttgga atgtatcgaa ttaggctata cttcagtaat gttcgatggt
     5341 tcacatctac catttgaaga aaacttgaaa ttagctaaag acgttgttga aaaagcacat
     5401 gcaaaaggta tctcagttga atgtgaagtt ggttcaatcg gcggagaaga agacggaatc
     5461 atcggtaacg gcgaattagc tgatattgaa gaatgcaaac aaatggttgc tacaggtatc
     5521 gactacttag cttgcggtat cggtaacatc catggtcaat atccagaaaa ctggaaagga
     5581 ttagcttttg accacttaca agcaattgct gatgctgtag gtccagacat gcctttagta
     5641 ttacatggcg gatcaggtat tcctcaagat caaatcgaaa aagctatctc aatgggtatc
     5701 tcaaaagtaa atgttaacac tgaattccaa ttatcatttg ctgcagctac tcgtaaatat
     5761 atcgaagctg gtaaagataa agaaggtaaa ggattcgacc ctcgtaaatt attagctcca
     5821 ggtaaagcag caatcgttga agatgctaaa tctcatatcg actggttcgg ttcagctaac
     5881 aaagcgtaag catccacata acagatacga tatataggaa tggagaacga cttttgaagt
     5941 cgctctccat tcttttttac ttaaaacggc agaccatgct cgtttctttt ttttatctat
     6001 gataaaatac aagcatatcc atgaaaaaag attaataaac aaggtggaaa cagatgaaaa
     6061 aaatcgtaat caaaggaaac cgacctttag ctggtgaagt aacgatcagc ggtgcaaaaa
     6121 atagtgcagt cgcattgatt ccagctgcaa tcttagctga ttccccagtg atcttggaag
     6181 gtgtgccgga catacaagat gttcattcat taattgaaat tttagagatc atgggcgcaa
     6241 ctgtccattt ttccaacaac atactagaaa tcgatcctcg agaagtcgta tcgattccga
     6301 tgccgcacgg aaaaatcaat agtttgcgtg cttcttatta ttttatggga acactgcttg
     6361 gtaaatttgg cgaagcggta gtaggcctgc caggtggctg ttatttaggc cctagaccga
     6421 ttgacctaca cgtcaaagga tttgaagctc taggtgctga agtgacaaat gaacatggtg
     6481 ccatgtattt acggacagaa aataaaggat tgcgtggcaa tcggattttc atggacgtgg
     6541 tttcagtcgg agcaacgatc aatgtgatgt tagctgctgt aaaagcaaaa ggacagacag
     6601 taattgaaaa cgctgcacga gaaccggaaa tcatagatgt ggcaacttta ctgaataata
     6661 tgggggcaaa agttcgagga gcgggtacag atgtgatccg tatcgaagga gtcgaaacat
     6721 tacatggctg tcggcatttc atgattcctg accgcatcga agcaggaacc tatctagcat
     6781 tagcagctgc agtaggaaat ggtatcaaag tgaaaaatgt gatctttgaa catttggaaa
     6841 gctttatagc aaaattgcag gaaattggtg tccatatgaa aatcagcgaa gatgaaatcg
     6901 aagtttaccc ctcgaaagaa ttaaaacccg caaacatcat gacctatcct tatccaggat
     6961 ttgccacaga tctgcagcag ccacttactg cgttactatt gatgactaca ggcacttcag
     7021 aaatcatcga tacgatctat gcgaaacgtg tgaaccatgt gccggaacta gcacgaatgg
     7081 gcgctgatat taccgtggaa ggaaatatga tcatcgtgaa tggtccaaat aaacttcacg
     7141 gcacagaagt cgtggcttct gatcttcggg caggtgcttg tttagtgatt gctggactat
     7201 tggcagaagg gacaacgacg atttacaatg tagattatat cttacgcggt tatgatcata
     7261 ttatcgagaa actaacagcc ttaggggcat caattgaaat gatagaggaa gtgggagctg
     7321 aatgagcgat tatttgacaa tggcagaact agagaacaaa acgttaaaag aagtttatag
     7381 ttatgccaaa gaatttaaaa ttccttatta cagtaagatg aataaaaagg aattatctct
     7441 agctgtgatt cgtgcacaag cagagaaaca aggattttat tatatggaag gaatattgga
     7501 tatcgttggt caagatggtt acggtttttt acggccaatc aattatggac caagtgcaga
     7561 agatatctat atttctgcct cacaaatcag acgattcagt ttgagaaatg gggacaaagt
     7621 ggcaggaaaa gcccgtccac ctaaagaatc ggaacgttat tatggtttga tgcacgtgga
     7681 aagcgttaat ggaaaagatc cggaagaagc aaaagaacgt ccgcattttc cagccttaac
     7741 acctctttat ccgcaaagac agcttcaact ggaaacaaca ccgcaaagac tatctacccg
     7801 aatgatcgat atcttttctc caatcggttt tggtcagcgt ggattgatcg tcgcaccgcc
     7861 aaaagcaggg aaaacttctg tattaaaaga aatcgcaaat ggtatcaccg aaaactatcc
     7921 ggaagtagaa ttaatcgttc tactgattga tgaacgaccg gaagaagtaa cggatctaga
     7981 acgaagcgtg aaaggcgacg tggtatcttc tacatttgat ctgcagccgc aaaaccatac
     8041 tcgagtttct gaattagtat tggaacgcgc aatgcgttta gtcgaagata aacgagatgt
     8101 cgttatcttg atggatagta ttactagact tgctcgggcc tataacttag tcgttccacc
     8161 aagcggacgt acattgagtg gcgggattga cccagccgca ttgtataaac ctaagaaatt
     8221 tttcggggca gcaagaaaca tcgaagaagg tggcagtttg acgattttag cgaccgcttt
     8281 agtcgataca ggaagccgta tggatgacgt gatctatgaa gaattcaaag gaactggaaa
     8341 tctagaatta caactttcac gtgaattatc tgaacgtcgg atcttcccag ccatcgatat
     8401 caaaaaatca ggtacccgaa aagaagaatt attgatgcca agtgaagaat tagaagaaat
     8461 ttggaaactt cgaaaaaata tgactggaga ttcattagaa tatacggaac aactgatccg
     8521 ctttttgcgt aaaacaaaaa acaatcagca atttttcaaa gaatttcaag atgtctcctt
     8581 tggtaaaaaa aatccgacca gaaatctgaa aagataattg caaccttgac tgaaacatgt
     8641 taagatgtta atgttgtaaa tcgacaaatc aactctggtt cagcaaatgg agcagggcaa
     8701 aggagcgaaa aaatcatgaa agaaaacatc catccagatt accacccagt agtattcatg
     8761 gattcaacaa ctggtttcaa attcttgtca ggttcaacaa aaacttcaca agaaacagtt
     8821 gaatgggaag acggtaatac atacccagtc atccgtgtcg aagtaacttc tgactcacat
     8881 ccattctaca ctggacgtca aaaattcaca caagcagacg gacgtgtgga ccgtttcaac
     8941 aaaaaatacg gtctcaaaga cgcaaacgct gcggaataat aaaaacgctg ataactcaac
     9001 gtttagcaga ctatctgata gggatagtct gtttttttat gttttttgcc aatttaaaac
     9061 ttcgtttgat gctgaaaaag tcgtaggatc ataatgcaca aaacaatgaa aaactagtat
     9121 atagttttaa ttatccactt gataaaaaca ccttatatta acgaaatgaa gaggggttat
     9181 ttcgaagtaa aaaaccattt acctaaaatt agaagctaag ctttcctttc aaggaaggct
     9241 ttttgttttg ttatagactt gcgattgttt gtgagctaga tcgttcattt taatgactta
     9301 tacacgcgga tgatgggtct ttttcctctg ttcattcata atattgaacg gtacagctag
     9361 cgatgctata ctgacctcag ttccaagaaa acgttaacat tttttgtggg ggagaggttt
     9421 tttatgaggt ttgatcaatt gatcgcagaa gaactgattg ttttcgatcc atcgatagat
     9481 tcaaaaggat cattatttga gcgaataggg gatgtgttga aaaaaacaaa taaggtaaga
     9541 aatgttcaaa aagtcgttaa ggcactattc aaacgagagg aggagatctc cacaggaata
     9601 gaagatggat ttgggattcc ccatgcaaaa agcaaatatg tgacaaaacc agcactgtgt
     9661 ttttttcata cagccaaatt aacggactat agcgggatag atgaaatgcc aattgaatat
     9721 atttttgcca tcgtagttcc tgaaaaatca gcagatatgc atctagagat tctcagcaca
     9781 ctctccagac gattgatgaa tgcagatttt agaaagggaa taaaagaagc cactacgcca
     9841 gcagatatca tcagacagat tagtcaagaa tgaaaatagt agagtctttg tccgagtatc
     9901 gatagtgaag aaaaaggagg aaaataatca tgaaaatcgt tggtgtaact gcttgcccga
     9961 ctggaattgc tcatacttat atgtcggcag aaaaattaga agtaacagca agaaatctaa
    10021 accatcaagc aaaattcgaa acacaaggtg taaaaacaga aaatcagtta tctgaacaag
    10081 aaatcaaaga ggcagatgca atcattcttg cagtagataa agaaatcgaa ttggaccgat
    10141 ttgccgggaa aaaagtaaag cgtgtctcta caagccgagc aatcaaagaa ccgcaagtag
    10201 taatagacga agcattgaga gatattggcg tattcgttgt gtctaatgaa aaagaaccag
    10261 ctgcaaatga gaaaaagccg acaatctata atcatttcat gaatggtgtg aattacatgc
    10321 ttccatttgt gatcgcaggc ggaatcatca tcgcactgag ttttgccttt gggattacag
    10381 cggctgatcc caactcagca gactataatg tgttagcagc tgcattttca aggatcggag
    10441 gagatacagc gtttgccatg atggttcctg cgttaggcgc ggggattgct acatcgattg
    10501 ctggaaaagc aggttttgcc ccaggaattg ttgccggatt actggcctct acaggtggtt
    10561 caggttttct tggcggaatg attggtggcg tgctagcagg gtatatctgt gattttctgg
    10621 caaataaaat tagcgtaaaa aaagaatttt ctgctattta tcagctgatt gtcgtcccct
    10681 ttttatcgat cttaattatt gggcttttga tggtatttgt gattgaacaa ccgatcacat
    10741 gggtgttgga tgctttgaca aattggctga atagcttggg aaatacatcc gggctattgt
    10801 tcggattact tatcgggatc atgatggcag ctgatatggg tgggccgatc aataaatcga
    10861 tttcgacttt ctcgatcggt ttgatgtcag ctggagtaaa tgcaccgatt gctgcttgta
    10921 tggctgcagg aatgacacct cccctaggta ttgctttagc cactttatta ttcaaacata
    10981 aatttaccaa agaagaacgg acttcaggtc aatcatgctg gattttaggc gcttcgtata
    11041 ttacagaagg tgctataccg tttgcggtat ctgacccgat ccgcgtgata cctagtttga
    11101 tgacaggttc agcagtcgct gcagggattt ccatggcagt aggagttact tcaatggcac
    11161 ctcatggagg gatttgggtg atgctaattc caaatgttat gaacaattta cctatgtatg
    11221 tcttggcaat tgcatcaggt acagtcacta cagcctttat gcttggacta ttgaaaaaaa
    11281 cagtgaaaga gaaaaaacaa gacaagtgga cgaaaaatga aaatctaaca aatgaggtgg
    11341 aagcgtaatg ttatatacaa tgaaagagtt attgaaagtg gctaaagaga atcaatttgc
    11401 agtaccagcc ttcaatattt gcagctatga tatgatgaaa gcaatcatcg aagaagcaga
    11461 gagattagaa gcaccagtaa tcttagaaat tcatcctgat gaaatcgcct atttaagtga
    11521 tgagtttgtt gcggcagtaa gagcctatgc ttatacaagt aaactgccga tcgttatcca
    11581 cttggatcat ggcggtacga tagaagatgt cttacgggcg attcgaaacg gctattcttc
    11641 cgtgatgatc gatgcgtctt tgaaatcatt cgacgacaat gtacagatta cgaaaaaagt
    11701 ggttgcgtta gctcatgcgg taaatgtctc agtggaggct gagcttggaa cgattgggaa
    11761 taatggttct gctgaaggag gtgctgaaac aatcatttat acagatcctg atcaagcgaa
    11821 gatttttgtg aaagaaacag gaatcgatac acttgctgta gcaattggaa cagcgcatgg
    11881 cttgtatccg aaaaacagaa ctccaaaatt aaatatcgac ttactgaaag aattgaacca
    11941 acaaatcgat cttccatttg ttttacatgg cggttcaggt aatccggacg cagaagtcag
    12001 cgaagcagta aaatacggag ttgccaaagt gaatttgagc tcagatctta aaagcgtctt
    12061 tttcaatgct ttgagagaaa tattgagtga aaatccatcg atgtacgagc cgaatgcaat
    12121 ttatccagca gctaataaaa aggtacaaga agtagtcaaa cataaaatgg aaattttgaa
    12181 cacagtaggc aaggctagtt tatactaaaa ataaaaatgt gtggtcaaga gggtatgaca
    12241 atagtcttgt cataccctct taaccacaca cgatgttcag aagtagtttt ttcgaaaaat
    12301 ttcttcttat ttctcgaagc caatcacttc tgtcacaacc tcttttttcc tgttactata
    12361 taaagtaaat ggggtgattt tatgttgaat gaagtggaga atagacagca ggagattgtg
    12421 aattatctaa aaaacaacca atttgcaaga gtcaccgatt tgatcgagtt agtaaattat
    12481 agcgaagcca cgattaaacg tgaccttgtt tttctggaaa aaagtggatt gatccgtaga
    12541 gttcgcggtg gcgccatgct tattgacaat cgaaaaattg acgtgcctta cctgatgaag
    12601 atcaccaaat tagaagaaga aacagaaaaa cgtaagattg cagatacagc ttcttctttg
    12661 attcgagatg atatggtctt attcttggat tccagtacaa cctctttaca cttagtcaga
    12721 aacctagcaa gatttgatgg tttgcagatc attacgaatg gcatcgtcac agctgctatg
    12781 ttgagtgaat atacaacggc aaaagtatct gtattgggag gggcaattgt tccgaaacga
    12841 gcaacaatca acggttcaaa agcctataac gatgcattgt catattatgc ggatttagcg
    12901 ataatgagtt gtcgcgggct agatttcagc caaggagtaa cagaaactca tgaaggagag
    12961 gcaatggtca aaaaagcttt tagaaaacaa tcagaccggc ttgccgtttt agttacacag
    13021 caaaaaattg cacataaatt catgcatcaa gccattgctt ttcatgaaat cgattatctg
    13081 gtgacaagta aagaactgac aaaagaacag tatgcgaaag taaaggaaca ccatatcgaa
    13141 tgtctgtacg gataaattat tacgtgaatg ttctaagtgg cttcctcatt tttagatagg
    13201 accttatctt tgctcgcttc tctcagccga tggtataata atgaaaaggt tatcttgtta
    13261 gataatctta cttttttatc caggagaaag gagtgcagaa aatgaaacat gtgaaggcgt
    13321 tggtagtgaa ggcaatcatg atctgggcga ttctgtgggt cgtgctgaca ggtttatacg
    13381 gtgttagctt tatggattca acgattgttg gggtaattat tgtagtcatg atctatgtgc
    13441 taggggatct gttgatctta cgtaaagtag ggaacatcgc agcgacgatc gcagatgctg
    13501 gttcagctgc agttatcttg tggctctatc tatattttat gaatgataca gtcgatattt
    13561 ggatgaaagt attgattcct gctctattga tcggtgtggc agaatggttc ttccacaagt
    13621 ggttgctaag tcaaggcatc gtaccagatg aacggaaaat ggaataaatc aacagttaaa
    13681 ctattcttat cgataaatga aagctttaaa aataaataga tgtttaatta agaataatca
    13741 taaactattt ttactcaaaa aaccgctcag attattttca gatctgaacg gtttttttta
    13801 tctattcatc aagatcaaat ttcatttttc aatgattcaa tcaattcgcc ctctgcttca
    13861 tgactatgtt ttcggccatt aacaagatag agtgctgtac atatcaatgg aatgactccg
    13921 gcaatcacat acaatcctct aaatgaagta agagatttta gcatccccat caaataagga
    13981 ccaacgccta agcctaggtc taaaccaata aagtaggtag ataaagcaat actgattcga
    14041 tgttcgcgga cgatttttaa acaaacagcc tgtccatttg acataaacgt accataacct
    14101 aggccgacta atgctccaga gataagcaat gtcacactat tcgtcgttac actcagtaaa
    14161 aataatcctg ctgttaagaa taaatagcta ggatacatca cgtattgttc tcctttgaca
    14221 tcgaaaatcc ttccagtcaa tggacgtgta aatgtgatga ccaaagcata tacgacgaag
    14281 aaaaatgacc cagcagcaac taagtggatt acttttgtat aagaagataa aaatgataaa
    14341 acacttgaat aagaaagacc catcaaaaag gcaattcctg taatgaaaag tgctttcttt
    14401 tcaataaaac tattgatatt ccatgagcga agagatattt tttgttcttc agataatgga
    14461 atatttttta caggaaagac aaggcaggca atcgtaacaa agaagaccag tacaattgaa
    14521 aaccagataa tgaaacgaaa atcagtcata ttcagcaaaa tcattcctaa aaatggccca
    14581 acagcggcag ctaaactagt acttaatcca taataattga tgccttcccc ttttttagat
    14641 gcagggatat atgcagtaac gatagcattt gtagctgtag aaactgtacc gtagccgaat
    14701 ccattcaaga atcgaactaa atacaatgcc ccgatagttg gcgtataaag atatgctaat
    14761 gttgaaagga gatagaagat cgcgccgcct cttaaggtaa acttccgacc aaatagttct
    14821 aactgtttcc ccatgaataa gcgtgccagc aatgttccga tgatatagat cccagaagca
    14881 aaacctgctt ggctcaagct ggcgtgtaag gtgtcttgcg caatgactgc catgatgacc
    14941 atcaacagat aataaatcag gtagacaata aaattgatca atgtgattcc aataaaatat
    15001 ttattaaata atttcttttc catgtatgtt atacattccc cttaagctga atatgctttt
    15061 aatttttgta tttgaacttc tacatcgtga taattggatg gatagcgatc aacaagttgg
    15121 cgaataaata aatctcttgt agacataaac agatttaaaa attcttcgca ttcgtttgtt
    15181 ggattctcat ccactgcttt gatcaattcg atcgagtttt gatacgcatc atttaaaaag
    15241 actaaagaag ctttcatatc aaatcttgtt tgatatgctg tttctaatcg attaatgatt
    15301 gtgttcatcc agtaattcat gttttttccc tctattctta aaaaatttta cttctgcagt
    15361 ataacgaata aaaaaaagga aaaaaatcgc ataagtgatg gaaaaaggta cttaacaaat
    15421 gttaatatca ttagtaaaat acctttggac tttaaaaagt tttaaagtac tgtatgatct
    15481 ttttagatga aagtttggat gggggagcat cattggatag tagagtctta aaggaatatt
    15541 tggataaaaa tgattttccg attatacgca aaggatatca taaatatttg acattcgaag
    15601 ggatggaaga ttcccatacg tatatcttga aagagggaac ggtaaaagca agtgttattt
    15661 caaatgatgg gagagaattt aaccttcgct atatcaaaga tcttgaaatc gtctctcttt
    15721 taaaagacga atattcacaa ttcatcgatt cgccgtataa tattcgagtt gaatcagaat
    15781 atgcagagtt ttatcgaatc aatcgtgtgc gcttttggca aaatatcaac caagacccaa
    15841 agctacaaca ctatgtaaaa gagtattacc gctatcgtct gctttattcg atgaaaaaaa
    15901 tgcagcaaat gctattaaac ggaaagctgg gagctgtttg tacgcaactt tacgaattat
    15961 atgatttatt cggtgtgcgg acagaagaag ggatgctgat cgatttttcg atcacgaatg
    16021 aagaaatcgc caaattttgt ggcatctcaa ctgctagcag tgtaaacaga attttaaaac
    16081 aactgaaaaa agatggtatt atcaccgtta aagaccatca tatcgttatt ttgaatatgg
    16141 acttgttaga agacaatatt atcttttgat gaacaaagag gttgtgaaat cagcgttttg
    16201 acctgagtat tagagaaaca aacggaaaaa tagttcctca tattttgccg ttttttgagc
    16261 taataccgag ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc ctgccgttta
    16321 actctgagtc acaaggaaca atttcaaaaa acagcttttc atgtttgatg aatattgtga
    16381 cttgtgaccg aggagttggt aggagaacac cgtttaccaa gaggttgtga aaaagctgtt
    16441 ctgcatcaag taataagaag agccaagtaa aaacagtctc ttatgttttt aacttggctg
    16501 gacttatcac cgtagatgta agcttttgaa cgccgtttat ttgcaacaaa aggggctgtg
    16561 acacgatttt tgtcacagcc ctttttttgc gaacattcat ttagcaattt ttagaaactt
    16621 ctgccgccac cgccaaaagt cccgccaccg ctagaatggg tagtacttcc gccaccgccg
    16681 cttccaggag gtgggctttt ggggattctt cttgtcgtga caaaagaatt tgtcagacga
    16741 tcggtttttt cagttaattt gatactggat ttttctcgga atggatattt gtagatgcct
    16801 gatttcaatt gataacgcga aacagtcaca aaataaaaga caagactaag gataagagcc
    16861 aaaacaacag caatgatgat ttctactgtc gtcaaaacct tatagcgtgt aattttgcca
    16921 gtttcttcgt ctacacgata atgtcctcct ggaactccgt cttccacata tgttgctgat
    16981 ttagacaggt aagcttttgc agcattgtaa taggcagcat ttgtcatctg atcataaaca
    17041 tcatctaaga tcgaatcgat tctgctatca gtcaaataat cgatcatatt accagaagtc
    17101 gaaatgtaga tttcacgctg tcccatatct aatagcaata ctgagccatt ttgatcattt
    17161 ccgactgcgt ctcttaagta attatcagca aagtctcttg gttcttctga attagatgta
    17221 gtcgttacga tcaagactcg tcctttgatt ttttcattca gcttttctgc ctgttgttcc
    17281 aaagattgga tttgatctgt tgtgaataac ccagcctcat catttaccgt agctgtatct
    17341 gcatgagtag ccaccggcca gccaacactt actagcaaaa gaaagaataa aaaaaatttt
    17401 ttcatcatat caagtaacct ccgatcagaa agaggatcgc tagcccagcg aaaatcccaa
    17461 gagcggtcag tcccaatttt ttctggctga tcggcaggac accgctgacc tttcctgttt
    17521 gtccattcat tgcgtagtag tagacttttt tacttgagtc attgcttcga taagtaacaa
    17581 gccataccgg cagtaatacg taattattta cttcgtttgt gattgaagca gaagttcgca
    17641 caccggtcaa tgttgtatat ccatttgcgg tatcttttaa cagcttctct gaatagtctt
    17701 ttaattcaga ttgaatctgt gatttgattg cctcatattc gatatctctt ttttctgctt
    17761 ggaacccggc taaatactgg cttttaaaat caattgcttt atctaacggg aaaggttgca
    17821 cgccttcgac catcttttgt tgcgtatttt ttgataaggc attttttact agttctttaa
    17881 aagaaagctt gcctgctctt gaaacagcaa attgcttcgt ttctgtgtat tcgatgtcac
    17941 ctatccgcca tatgcggata actgtaccat tcgcctgcat ggcaccatct acttcagcat
    18001 ctgtagccca ataagggaaa tacacacctg tcaacttatc gatttgatcc ttattgaaaa
    18061 aagctttggg aacaaaccat tttttcttgg tccaagcgag aaatctgttg attgcttctt
    18121 ctttctcgac agcaaaagga agtactttat ttggcaagaa ttctccgctt aaacgtcccg
    18181 atagtacgac gggattatgg cagtagtaac aataagtcgc agcagttgtc gcatcagtca
    18241 cgatctctgc accacagttg ggacaaagaa aaagatctat cgctgcggta ttatctgcag
    18301 attcgtccgc tgtatctgaa aaaccgcctg cctcgtcaac cgctttgcgc tcggcttcat
    18361 tcatatcggc aagctgttct tgggctgtaa aagtaaaatc ttcggtaggt gaaggttctt
    18421 ctttgctttc ttcgccagcc atacgcgctt ctttttgctt ttgctcatat tggctgactt
    18481 cttcttctgt atagatattc aggcagtatt cgcaatgaaa tttttggtct ttgggatcaa
    18541 aagtcaacgg accgccgcaa ttaggacatt tatgtgtaag aacatccatc agctgcacac
    18601 ctttctaaat aaattaatta acggggattc ctttaaacgg tttaccacag tttggacaga
    18661 atttagggat gccgttggaa agatccacga tctcattgca ttcactgcat cgcatttgta
    18721 gcttaggctg aacgttttct acaggtttgg cagctccgca gttggaacag aattttccgg
    18781 aattttctgt cccgcaaact ggacatgtcc aagtatccgc atttccttgt gcagtttgtt
    18841 gattttgttt ttctgcttgt tgtttgattt gttcttggtt attttgagct gcttgagaaa
    18901 gataagaacc atttgcattc attcccatac ctacacccat aaagccggtc attgctcctg
    18961 cttcattttt tccggctgct tccataccac gtgcaatgga accttgcacg tatccttcac
    19021 gtacgctagg atcacctagc atcgcccctt cgttacgcat attgatcagt ttcacagaat
    19081 catcggtgta agaaatgctg gctacagcca cagaaacaat ctccatccca cgtaattcac
    19141 gccaagaatc atccaatgca gtatccatgt atttgcttaa ttccaaactc ttagatggta
    19201 cataagaaat ccgttgacca tctgcagaca tctgattgat tgctgattgt agcgctgtta
    19261 ggaattcagc taaatactgc tcattgatat cattgatttc cacttgtgtt ttgtttttag
    19321 ggattgcgtt tgtataaaat aaaatcgggt caacgacatg aatagaataa gtgccatgtg
    19381 cccgtaagaa taattctgcg ttatagaaat tgtcaaagta gttcaacggc gcgcttgtac
    19441 cgaatttgat tccctttatt tcttggagat tgatataaaa aacttgttgt ttctgcggtg
    19501 tgacgccgcc aaatttgaat cggtcaaaag tttcagcaat tgcttctttc agtgttccat
    19561 tgaacatgga aggagctgcg tcatttttaa tggtgtaata accttcctca gcggtataat
    19621 cgatgatttt tccaccatcg accaaaagca tcatcatgtt tggatagaca tggacgactg
    19681 ttccgtctgt taaaacatct tctgttcctt tacgattgga gccacgtttg tcatctttgc
    19741 ggactttgac accttttgtc atgaccgttg tatcactcat attgtctggt tcgatgactt
    19801 ctagccattg atctgccaga ccgccgccaa ccatacttgt tgctgctttg ataagaccca
    19861 taaaaaattc ctcctctgat tcagctagtg ccaaacaatt attgtttatt tattatacca
    19921 taggaagatg tgaaacttct cggactagag acggaattga aatggaaaat tgcgtattgt
    19981 ttaaatacct gaaaaagccg agaaggatag tagttttttt acctaatatc acgtataata
    20041 agagatagtg aattttttaa aaaaggggga tccttatggc atatcaagca ctttatcgag
    20101 tctggcgttc gcagcgtttc gaagacatcg ttggacaaaa agctgtgaca cagacactaa
    20161 aaaatgctat tgtttcccac aaaacctcac atgcctactt gtttacagga cccagaggaa
    20221 ccggaaagac gagtgcagca aagattttcg caaaagcaat caactgtccg aacagtaaag
    20281 acggtgagcc atgcaacgaa tgcgaaatgt gtcgatcaat tactgctggt acgcaagaag
    20341 atgtgatcga aatcgatgct gccagcaata acggagtcga agaaatccga tttatccgtg
    20401 atcgggcaaa ttatgcgcct acaaaggcag cgtacaaagt gtacattatt gatgaggtac
    20461 atatgctttc aacaggagct ttcaatgcct tgttgaaaac attggaagag ccaaaagaaa
    20521 gtgtcatatt catcttagca acgaccgaac ctcacaaaat accagctacg atcatttcac
    20581 ggactcagcg atttgatttt aaacggatca atacggcaga tattgtcgaa catcttgaat
    20641 ttatcttgaa agaatcgact atcagctatg aaccagctgc attgaatgtg attgcccgtt
    20701 ctgcagaagg cgggatgcgg gatgcattga gtattcttga tcaggcaatc tcgtttagtg
    20761 aaggaacaat tactttagat gatgccatgc aagtgacagg tagcttaact gacgaaatga
    20821 tggacagtta cttgagtgct tgcgtgaaaa aagaagtacc agaagcattg gaaacattga
    20881 accaaattct cgcagccggc aaagaatcac gtcgctttct agaggatatg ttacaatact
    20941 gccgtgactt gctgatgtat cagcaagcac ctaacctagt tgaggaacaa tcgagtacga
    21001 tcagaagtac atttacagaa ttggcacaaa ctgtttcagc ggatcagctg ttttcaatga
    21061 taaagatttt gagcgatact caaaatgaaa ttcgttttac caatagtgcc gctatttatt
    21121 tggaagtcgc tacagtcaaa cttgcacagc taccgtctgc ttctgttcct tcagcagatg
    21181 tagcatttgc agatgatcag atagtaaaac agctacaaca agagattaag gaactaagaa
    21241 acgaaatcaa ggaactacgc agtaatacag gaattgagaa tacaaaagta cagccggtac
    21301 agcaaacaaa tcataaaaaa cagacaagta cctatcgtgt accgacggaa cgagtatata
    21361 aagttctgcg ggaagcgaca agacagcatc tgaaccaagt aaaagaagta tgggaagact
    21421 tgctgatgag tctatctgtc actcagcgag caatgcttaa agcaagcgaa ccagttgcag
    21481 ctggacctac aggactagtg attgcgtttg attatgagat tgtttgtcaa agagcagcaa
    21541 atgacgaaga attgcagctt tcggtacaca acaacttaag ccgaatgatc aaagattatg
    21601 cgcctgatcc tgtttatatt acaagagaaa gctggccaga acttcgaaga aattatttga
    21661 cgcaggcaca agaagaggga acagaaaact cttttgcttc tgaagactca gaaggagaaa
    21721 tcgagttgct tcctcaagaa caagaagtac cagaagaaac acttgtagta aaagcagagg
    21781 aactgtttgg ctccgaagct gttacagtgg tagatgatta ataaaaagga gtagataatt
    21841 atgatgcgtg gaatgggcaa tatgcaaggc atgatgaaac aagtacaaaa aatgcaaaaa
    21901 gaaatggcag aagcacaaga gctgctaaat aaaacagagt ttacaggtaa agccaccaat
    21961 gaattggtca aagcggtttt tacaggcgat cgtcgcatga aagatatcca aatcaatgaa
    22021 gaagtcgttg atcctgatga tatcgagatg cttcaggatc ttgtcgtaat ggcagtcaat
    22081 gatgcgatcg cacaaattga tgtggaaact gagaaaaaat tagggaaata cgcaaaaggt
    22141 ttgccaggat tttagtttta aactgaagga gtaatgaata tgcaatatcc agaaccaatt
    22201 gcgaaactga tcgacagcta catgaaactt ccaggaatcg gtcagaaaac agcgacaaga
    22261 ctcgcctttt atacgatcga tatgaaagat gaagtagtaa atgaatttgc taaatctttg
    22321 ttaagtgtaa aacgcgattt gcatttttgt agtatctgcg gaaacatcac agaagaagat
    22381 ccctgcgaaa tatgtagaaa tccttcacgt gatcgaagcg ttatcctagt ggtcgaagag
    22441 ccgaaagatg taatggcaat ggaaaaaatg cgagaatatc acggacttta tcatgttctc
    22501 catggtgtgt tatcaccgat ggaagggaca ggtccagaag acatcaatat ttcttctctg
    22561 ttgcagcggc tgcatgatgc agatgtaaaa gaagtgatta tcgcaacaaa tgcaacaaca
    22621 gagggagaag caacagctat gtatctctca cgtctgatca aacccgcagg tatcaaagtg
    22681 actcgtctag cacatggtct gtctgtaggc tctgatatcg agtatgcaga tgaagttact
    22741 ttattgaaag ctgtcgaagg aagacgtgag ttataaattt ctaattaagt gttttttcag
    22801 atacaatata aaagcagcac aagagatggg ggcaacaatg tgaacggact atttattaca
    22861 atcgaggggc ctgatggtgc aggaaaaacc agcatcttga atgaacttta ccctcttttg
    22921 aaagaagtag caaaaacaga aatcattcaa acaagagaac caggaggcat tccgattgct
    22981 gaagagatcc gagcagtgat ccttgatcca aaaaatgatc ggatggatga acgtacggaa
    23041 gcactgcttt atgcagcagc aagaagacaa catctagtcg aaaaggttct gcctgctctg
    23101 gcaaaaggga aaatcgtttt atgcgataga tttgtcgaca gttctcttgc ttatcaaggt
    23161 gcaggccgac gcatcggcgt taccgagatt gctcggttga atgagtttgc tacagaaggt
    23221 actacgccgg attttaccct ttatcttgac gtagattcag acaccggatt aagaagaatc
    23281 aagaaaaaca gacaaaatca aatcgaccgt ttagattcgg aaggtttgga attccaccag
    23341 cgtgtacgtc atgcttactt aaagctggca gaagagaatc cggaaagaat ccacaaagtt
    23401 gatgcaagaa aaagctttga agaagtgctg caaacaagtt atcatacaat tattgaacag
    23461 tatccgcaat tttttgaaaa ctaggaaggt gaacgtttat gaaaattatt ttagcgatta
    23521 tccaagacaa agacagcaac cgtttatcaa atgaattgat tgatgcaaat atccgtgcca
    23581 caaaactatc atcgacaggc ggcttcctta aggctggaaa cagtacgttt atcgtgggta
    23641 ttgaagatga ccgtgtagaa gaagcattgg aaatcatcaa aaaaacgtgt gaatcaagaa
    23701 agcaatttgt atctacacct gttacactgg atatttcgat ggatgggggc gtcccttatc
    23761 cagtagaagt agaagtaggc ggtgccacag tcttcgtctt acctgttgaa ggattccatc
    23821 aattttagga ggagctgagc tatgaatcaa gaaataaact tggctcaaat gcaaccgatc
    23881 gtgtatcagc agcttcaacg gagttttgag catgggcgta tcgctcatgc ttatcttttt
    23941 gaaggggaga aaggaacagg aaaacatgaa gtaggtatat ggcttgcaca gcacctgttc
    24001 tgtacccaaa tgaaagacaa tctgccttgt ggaaaatgca acaattgtca gcggatcgaa
    24061 aagaaagaac atccagatgt tttggttatt gaaccggaag gacaaaccat caaagtcgat
    24121 cagatcagac gtttgcagac ggaattcagt cgcagcggtt acgaatcaag aaaaaaagtt
    24181 tttttgatca aagaagctga aaaaatgaat agcagtgcgg caaacagttt gttgaaattc
    24241 ttggaagagc cccctggaga ttttttagcg gtgttagaaa cagatgcagt aggaagaatt
    24301 ctccctacta ttcaatctcg ttgtcaagtt ctgcattttc aagaactttc gaaagaagca
    24361 ctgatccatc gattgcaaga agagcagatt ccattggaaa aagcaaaact tttggctttc
    24421 ttaacaaaca gtttaggaaa agcagttgaa atctctcaag atgaatggtt taatgatgct
    24481 aaggacttga tttatcaatg gttcgtttac ttgcaaaagc aagatactca agcctttatc
    24541 tatgtccaaa aaaaattggt aaaatcattt aaagaaaaaa cacaacagtt tacaggatta
    24601 tctgtcttga tgttttatta tcaggaagca cagcgtcaag cagtagcaaa cggcgaatat
    24661 ggcaaagtga aacagataaa ccagataatt gaacggatat tgatcgcaga gcagaaacta
    24721 cgcagcaatg tcagttttca ggctgttgcg gaacagttcg ttttgcatac gattttttag
    24781 cttgccgcaa aggatgatga tcaaggagga tggaaatggt agaagtagta ggtgtccgat
    24841 atagagatgc aggtcatatc tattattttg caccaggaga ctctgaatat gtttatagtg
    24901 acaaggtatt agtcgaatct cagcaatcct tacatattgc tactgttgtc cttcctaaaa
    24961 aagagattga tcccaatgac cttcctgaag ctatccgtcc gatcttgcat aaagcgtcac
    25021 aagctgaatt agaaaaagac aaaaaaaaca aaaaagatgc ttatgatgca ttgaaaatcg
    25081 caaaagaaaa agtgcagttg cgtgagttgg aaatgaaaat ggtcaaagcc gaatacacat
    25141 ttgatcgaaa taaattgatt ttttctttca cagcggacgg gcgaattgac tttagagaat
    25201 tggtaaaaga tctggccgct gtatttcgta ctcgtattga gcttcgacaa attggtgtcc
    25261 gcgatgaagc aaagataata ggcggaattg gtccatgtgg gcgtccatta tgctgttctt
    25321 cttttttagg cgatttcgtg cctgtatcaa tcaaaatggc aaaagatcaa agtttatctt
    25381 tgaatccaac aaaaatttcc ggtttatgcg gacgcttgat gtgctgtctg caatatgaga
    25441 gcgaagaata cgagacagcg aaaaaagaac tccctgactt tggaaaagta attgctacac
    25501 ctgatggtaa agggaaagtc gtagggttga atctgcttag ccgtatcgtt agtattcggc
    25561 tgactggacg ggaaaatcct gtcgaatatg aatgggatga attgaaagaa tttatccaag
    25621 taggtgagat gaatggataa gagatcatta tacgacggat tgaatcaatt agaatcagag
    25681 ttgagaaata cattaacaga acttgttgaa atgaagcatg ctttgcatga gatcgtagaa
    25741 aaaaatacaa ctttggagat ggaaaatcaa cgtctgcggg aacacctgcg ggaattgaac
    25801 caagttgcac aagctccagc tgaaaatgtg aagcaggaac tttctaagtc tcgtatgaat
    25861 cttgaaaaaa tttatgaaga aggattccat gtctgctacg atttatatgg atcaagaagg
    25921 gacaatgacg agccttgtgc gttttgtcta gaagtgatct atcgcgaaag cggtcgatga
    25981 ccaataacaa gaggagactg gagcggagaa atactgctcc agtcttttta ttaaggagat
    26041 tgtgacagat gtataatcaa aaaagtttca agggaaatcc ggtatatggg gatttatatc
    26101 ttgtaccgac gccaattgga aatttagaag atatgagcta cagaagtgtt cgtatgctgc
    26161 aagaatccga tcttatcgca agtgaggata cgcgtaatac acaaaagtta ttgaatcatt
    26221 ttgaaatcca tacaccccaa aaaagtttac atgaacataa ttataaagaa cgtgtccctc
    26281 aattgattgc tttgttaaaa gaaggaaaaa cgattgctca agtcagtgac gcaggaatgc
    26341 cttccatcag cgatcctgga catgagctag ttttagcctg cataaaagaa gggatttctg
    26401 tgatctctat cccaggacct acagctggga tgacggcttt gattgcttca gggctagtgc
    26461 ctcaaccatt tttattttat ggcttccttc cacgtaagaa aaaagaacag aaagaaacat
    26521 tgaaaaagct aaaagaacag acagttacgc tgatttttta tgagtcacct tatcggatca
    26581 ctgctacatt gacgaatttc ttagaaattt ttggaaatcg ccaagtcgtt ttgtgtcggg
    26641 aattgacaaa ggtccatgaa gaatacctta gaggaagtac ggaagagcta ctcgcttata
    26701 tcgaagaaca tccagtaaag ggtgaatgtt gtctgcttgt agaagggaat ttgttcgaaa
    26761 tgccagtgac ggaagttgat gaagagttgg gaacgttaaa ggaacaagta gaagcaaaga
    26821 tcgcttcagg cgaaaaacca aatgctgcta tcaaagcagt agcgctgaaa aatgggctga
    26881 aaaaacaaga agtctaccgt cagtaccatg aattagactg agatagtata agttgtctat
    26941 aatgaaagac taatgaggaa atcagctact aacttttcaa atctcttgaa cgcgaacgaa
    27001 tgttcttgta taatgaataa gaggtgagga tgatggagct gcaatttcct ttacataatg
    27061 atacatcaag aaaaatcatt catattgata tggatgcttt tttcgcttcg gttgaagaac
    27121 gagatcatcc tgagtttcgc ggacatccat tagtaattgc cagacacccc agtgacactg
    27181 gtgggcgtgg agtagtgact acagcaaatt atgaagcaag aaaattcgga attcattcag
    27241 caatgagtgc acaaaaagcg tatgagcttt gtccgcaagc catctttaag cctggcgacc
    27301 atcataaata cagcgatatt tccaaacaag tacgggaagt attctatcgt tatacagata
    27361 ttgtggaacc agttagtatc gatgaagcgt atcttgatgt gaccgaaaat aaaatcaatt
    27421 gcaagtcagc catcaaaatc gcccgaatga tccaagcaga tatttgggag agtgtccagc
    27481 taacatgttc tgcgggagta agttacaata agtttttggc caaattagca tcagactatc
    27541 aaaaacctag aggaatcacg gtagttgccc ctcaagacgc tgtgccattt ttaaaagctt
    27601 tgccaattga aaaatttcat ggggtaggga agaaaactgt acctagaatg catgagctag
    27661 ggatttatac aggaaaagac ctatatgaat gcacagaaat gatgttgatt cgaaattttg
    27721 gaaaaatggg ctattctctt tatcgtaaag tcagaggaat ccatgattct ccagtaaacg
    27781 ttacaagaga gcgaaaatct gtgggaaaag agcatactta tgggcagccg ttgcaaacgg
    27841 aagaagcagt gctgactcag ttgcgccaac tagctgaaaa agtagaagaa gcactgagaa
    27901 gagtccaaaa acatgggaaa acagtagttt tgaaggtcag gtacacggac tattctactg
    27961 tgaccaaaag agtgacattg ccagaatata tctataaaaa agaagcactc ttttatcaag
    28021 caagccttat atgggaggaa atcttgggag tagaaaaagg aatccgattg ttagggatta
    28081 ctttgacgaa tctggacccg atgacttatg aaaacatcgt ccttccatta tgggaaaatc
    28141 aagaaatata aaaacagctt tgagagttga aaccggaaat ccgttttctc tcccaaagct
    28201 gtttttatat caagtcttgc tttttgattt gttcataggt ttctgttgca tccgtacatt
    28261 tttcccaaat cacaacttga ttggattcta aagatttttg gacgtcaatg atccgctggt
    28321 tgctacttcc gcgaaattgt agttttaggt cacgtttgct taattcaaag cgtccgtcaa
    28381 caaggatatc gatttgcgaa aggagttcta atttatcttc tgaatcttgt agcaattcat
    28441 caaaggtata gcctgtccat gaccagatat cttttgtcgt accgaactct tttcggatac
    28501 gtcgtacgac ttgcagacaa acatctgtat tcaaaaaagg ttcgccacca agtaaagtta
    28561 atccttgcac gtaatcgttg cgcaagtcag cgatgatttg ttcttctaat tcaggtgtga
    28621 atggtttgcc atatcggaaa ttttgtgcag ctttgttgaa gcagccttcg caggcaaata
    28681 agcagccact aacgtaaaga ctgttgcgga caccttcacc atcgacaaaa ttaaacggtt
    28741 tgtagtcagc aatatagttt tggctgtagt cttttgctaa ccattctttt ggttttggat
    28801 ttctcataaa aaaacttctt tcatttaagg ttcaaagata tagctattga agataaaatc
    28861 tactcgacca cataaataag aacaaactga cctaaatcca ttgttcaaga aagcttgttc
    28921 ttattttgat agagggcgtt caaagataca aagccatcga aagagggtgg tgaaaaaaga
    28981 cttgtcatag cctcttattc tgaataaacg gtgttcaaaa gcttgcatct gcagtaataa
    29041 gcccaactaa acgaaaaata tgaggaacta tttttgttta gctattctta ttacttgatg
    29101 caggacagct ttttcacaac ctcatttcat gtgtttcaca cgtgaggaaa tttctttatg
    29161 gcggccatgt accatcggac gagcttgcgg atttcctaaa tagccacaag tacgtttgac
    29221 tacatcacat gtttttggat catgatttcc acattctgga cattcgaacc ctcgttctgt
    29281 cggtttgaaa tcaccttcga aaccacattg atagcaatga tcgattggcg tattcgtacc
    29341 aagatacccg attttgtcat atgcaaaatc ccatacagct tctaaagctt tcggattttg
    29401 ttggagcata gggtattcac agtagtggat gaatccacct gaacaatatt ttggataatc
    29461 cttctcaaat tccaattttt caaatggcgt tgggttttta cgcacatcat agtggaaact
    29521 gtttgtataa tattctttat ctgtgatatt ttcgatgatg ccgaattttt ctgtatccaa
    29581 acggcagaaa cggtcagtta gactttcact tggagttgaa tagacactga aatgataacc
    29641 atattcatcc ccccatgcat ctgcattagc ttttaattct ttgacgatat caagtgtgaa
    29701 agcttttgct tctgggtttg tttcccattc gccaccgaag aaagctgaag caacttcata
    29761 caatccaatg tagcctaacg atacagtagc tcgacggttt ttgaataatt cattgacaga
    29821 atcggtacgt gaaagacgtt tcccgaaagc accatacata tataaaattg gcgcatttgc
    29881 tggaatggct tctttacaac gttctacacg atagagaagc gcatctttca tgatttctaa
    29941 gcggtcagac agtaactgcc agaatttatt ctgatctcct tttgattcga tcgcgattct
    30001 tggcaaattc aatgtaacaa cacctaaatt cattcgtccg acatttactt cttgaccatt
    30061 ttcgtctttc cacccttgca agaatgaacg acagcccata ggtactttga aacttccagt
    30121 taattcaacg attttatcgt aattcagaat atcaggatac attcttttcg ttgcacattc
    30181 caatgctagt tgtttgatgt cgtaattagg atcttcaggg tttaagttca cacctttttt
    30241 caaagtgaag atcagtttag ggaagatagc tgtccgcttt tcactcccta atccattgat
    30301 tcgaatctgt aaaattgctt tttgaatttc tctttcaaac cagttttctc ctagtccaaa
    30361 tcctaatgaa gtgaaaggag tttgtccatt tgaagtaaat aaggtattga tttcatattc
    30421 caaactttgc atagcatcga agatatcttt tttggttttt gcttttgcat attctttttg
    30481 gcgttctggc gaatcgatcc attcttccgc gtcttttaga tgttttttat agttcaattc
    30541 tgcaaaagga gcaagcaatt catctgttcg gtcggcagaa cagccaccat attgacttga
    30601 cgctacattg gcaataatct gtgagatttg tgctgtggcg gtctggatag attttggcga
    30661 ttcgacttcg gcattgccga ttttgaaccc attgttcagc atgcctttga aatcaatcaa
    30721 gcagcaattc gtcatcggtg tataaggatg ataatccaaa tcatggtaat ggatatcgcc
    30781 tttttgatgc gcatttgcta tatgtggtgg caacattttc agtccgattg attttccaac
    30841 gatcccggct gtcagatctc gctgtgtatt gaaaacatta ctgtctttat ttgcgttttc
    30901 gttcacgaca gtttggtcct tgttgattag cttttcaatc gaaaagttga tatctgttgc
    30961 tttactgcga gcaaaatctc gtttggtacg ataattgatg tactcttctg ctaattcata
    31021 ttcgtttttt tctagtaata tatgttccac gatgttttga atctcataga ttttgacatt
    31081 ttcagcaaac cggcttttga tttcttggac aaccaagtct gcaatcattt gaattttttc
    31141 ataagtaagt ggtgataatg agccgtgaat tttcttttct gctttgatca gtgcttcatt
    31201 gatcttttgt tcatcgaaat caacatggcg gccatcgcgt ttgatgactt tcatctggga
    31261 tgtctctggc tgctgagtaa cgacagtttt ttcttcataa tttactttca ttatcttcaa
    31321 ctcctgctaa attgttcttg tctatcataa aacaaaatcg gggaaggagc aactaaatat
    31381 cggctatata tagtattcaa tgtgctgtat aattggattt ctatactata tgtagtgtat
    31441 attttattgt gaaaaaaggt gaaaacttgt agctggtgga tttgtttttt gcatatttaa
    31501 ttttgctgaa taaaataatt gaataaaatt ttttattcag tccaagtaaa agtgacaaaa
    31561 acataagagc aaattattcg tagatatgct actatgcaaa taaatagaaa tgaaaaatga
    31621 aagaggagga gaagaattgg aaaagatgat cgaagtgaaa catctaagta aaagctacgg
    31681 cagcaaactt tctcccgtca aagccttaga taatatctca ttcacagtag aagaaggcga
    31741 gtttgttggg attatgggcc caagtggtgc aggaaagaca acgctgatga atatcttgtc
    31801 cacgatcaat ttgcctacga tgggcagtgt ctcgatccaa ggaaaagaaa tcacaaaaat
    31861 gaaaaacagt gagctcagtg actttcgtcg aaaaaaacta ggattcattt ttcaagagtt
    31921 caatttgatc gataccctaa atgcaaaaga caatatccta ttgcccctag cagttgaacg
    31981 gatgactaaa gaggaaatgg aaaagagagt aagacatgta gctcaattgt tgaatataga
    32041 agaactgctc aaacggtatc ccgatgaact ttctgtcgga cagagacaac gaattgcagc
    32101 tgctagagca cttgttgttc agccacaagt gatttttgca gatgaaccaa ctggctcttt
    32161 ggactcgaag tcggctacag aattgttgaa ctacttgaag accatgaatc aaaaagaaaa
    32221 agcaacgata ctgttagtca cccatgatcc atatacagca agctactgcg atagaatttt
    32281 atttattaaa gatggtgtga ttttttctga agttgtcaga agagggacaa gaagagagtt
    32341 ctttgagaaa gtaatagaca tgcaggcgac gattggcgga ggaggaaaaa tgaatgctgt
    32401 ataaactagc cgttaaaagt ttttttcggc agtcacgagg ctatcttgtt tattttttca
    32461 gtttgacttt atccgtaatg atctattatt ccttcagcgc aatgacctac gatcaatctt
    32521 tagtcagaag agcaagtcaa gatacttcga tagatggtgg attgagtttt ggtggattta
    32581 tcattactgt tgttttgctt tttttcgtat tcagtgccaa ccgatttttc ttgaatagaa
    32641 ggcaaaagga aataggcatc tatcagcttt ttggtatgaa taaactgcag atatcaggta
    32701 tatatgtgtt agaaatcatg ataattggat tgttcgcctg tatttctggg atattactcg
    32761 gtattatttt ttccaaactc ttttcaatga ttttggttcg aatgatggat atggatctta
    32821 ctagtccatt ttttatctcc attccatcag tagtagatac tttatttgct tttttcatca
    32881 tccttttagc agtatctgta tattcaatat ggaaaatatg gcgctatcca atgattcgaa
    32941 gttttggaga aaaagaacaa gcagatagtt caacaatgcg cttcaggaca aggcaccgat
    33001 tactcgcagt catcgggtta ctacttttaa cgagcggata ttttggagct agtcatttta
    33061 gggaaatcac ttcatggatg atcagttatc attatcttga tacattagtt ttcttttttc
    33121 cttttttaat ttttttcgtt tgtgtggtcg gtacttattt gtttttctgt tatacatttc
    33181 ggttattttt gatcatattg agtaaatgga agatttatta ctatggtatc aattttttga
    33241 taatgggtaa cacgcaagtt catttgctca aaagttggcg tacaaattct ctgattacag
    33301 tcattctcgg aatttcttta atgacaattg gcggcacggc tagtatctct actattttat
    33361 cctaccagga gaaagtttcc tccccgatgg actatcagtt tgatgttgag acagagaaaa
    33421 aaatcaagcc gattctagca gaggaagggc agaagataac taaagaaatc accttgcatt
    33481 ataaagtcat tgggagtatc tatagtcaaa atctcgaaga acctatgttt caaactgcta
    33541 atctgatcac ggaaacacaa tatcaagcct tcagaaaagt taatccagat ttgccgaaca
    33601 tttctttgaa atcacctggt catactgttt tgcttgatca aatgcaaact ctttttcgga
    33661 atatttcatt atatggatct agtattggac tgccagataa tcaagaattg aagattcaac
    33721 aaatgcttcc aagtgtatta ggtgacgaaa caatggtata tctaagtcct gtactgattg
    33781 tagatgatac cgtttttgaa aaagcaaaag gtgtggatta tcaagtagtc aatgtagatg
    33841 tatcaggtgg aaataatgaa aaaatagatg aacgactgag caatgacctg tcgataaagt
    33901 ggctcaatgc tgtttattat tcttatgata tcgtaaatgg aagtctacaa ggagaaatta
    33961 gcacaaagaa acttccggat gaccaaatga aggaagcaca atttagccaa gaaagctctc
    34021 gtttgaattt ctcttccagc tattcgaaat tgcgtgcagt taatcggcga atcggtatca
    34081 atatttttgt cacactattt gtcgggatga tcgtgattat tgctactggt agtattttga
    34141 cagtccggca attagcagaa gcagaggaag aaaaagaaaa ttatcaccta ttgactaagc
    34201 tggggattcc acaaagaagg atcagacgga tgatcttcga gcaaaacgca cttaccttct
    34261 ttccacccat gatattaggg atcactcacg cagtatttgc aatcaatgtc ttcactcagt
    34321 atataaaaac tgccgattac tggttggctt atcttgtgag tggcttattg atccttatct
    34381 atcttttgct ttatgtcatt acgagtcagt tatattgtcg gattatcgag gaatgatata
    34441 cggaataaga caagataata aatagaact
//
LOCUS       DAGCYL010000011        33074 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.011, whole genome shotgun sequence.
ACCESSION   DAGCYL010000011 DAGCYL010000000
VERSION     DAGCYL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.011"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            19..876
                     /locus_tag="MB216_000388"
     CDS             19..876
                     /locus_tag="MB216_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBT4525249.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            991..1911
                     /gene="pstC"
                     /locus_tag="MB216_000389"
     CDS             991..1911
                     /gene="pstC"
                     /locus_tag="MB216_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBT4525250.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1911..2795
                     /gene="pstA"
                     /locus_tag="MB216_000390"
     CDS             1911..2795
                     /gene="pstA"
                     /locus_tag="MB216_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBT4525251.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2810..3616
                     /gene="pstB"
                     /locus_tag="MB216_000391"
     CDS             2810..3616
                     /gene="pstB"
                     /locus_tag="MB216_000391"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBT4525252.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3632..4390
                     /locus_tag="MB216_000392"
     CDS             3632..4390
                     /locus_tag="MB216_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBT4525253.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4403..5080
                     /gene="phoU"
                     /locus_tag="MB216_000393"
     CDS             4403..5080
                     /gene="phoU"
                     /locus_tag="MB216_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBT4525254.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5318..6349
                     /locus_tag="MB216_000394"
     CDS             5318..6349
                     /locus_tag="MB216_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBT4525255.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6346..7113
                     /locus_tag="MB216_000395"
     CDS             6346..7113
                     /locus_tag="MB216_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBT4525256.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7213..8781
                     /gene="liaX"
                     /locus_tag="MB216_000396"
     CDS             7213..8781
                     /gene="liaX"
                     /locus_tag="MB216_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBT4525257.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9078..9398
                     /locus_tag="MB216_000397"
     CDS             9078..9398
                     /locus_tag="MB216_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBT4525258.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9398..9748
                     /locus_tag="MB216_000398"
     CDS             9398..9748
                     /locus_tag="MB216_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBT4525259.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9915..10853
                     /locus_tag="MB216_000399"
     CDS             9915..10853
                     /locus_tag="MB216_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBT4525260.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10868..11698
                     /locus_tag="MB216_000400"
     CDS             10868..11698
                     /locus_tag="MB216_000400"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBT4525261.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11742..12767
                     /locus_tag="MB216_000401"
     CDS             11742..12767
                     /locus_tag="MB216_000401"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBT4525262.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12782..13720
                     /gene="galU"
                     /locus_tag="MB216_000402"
     CDS             12782..13720
                     /gene="galU"
                     /locus_tag="MB216_000402"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBT4525263.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13845..14285
                     /locus_tag="MB216_000403"
     CDS             13845..14285
                     /locus_tag="MB216_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBT4525264.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14288..14890
                     /locus_tag="MB216_000404"
     CDS             14288..14890
                     /locus_tag="MB216_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBT4525265.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14949..16055)
                     /locus_tag="MB216_000405"
     CDS             complement(14949..16055)
                     /locus_tag="MB216_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBT4525266.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16265..17266
                     /gene="ccpA"
                     /locus_tag="MB216_000406"
     CDS             16265..17266
                     /gene="ccpA"
                     /locus_tag="MB216_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBT4525267.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17603..19948)
                     /locus_tag="MB216_000407"
     CDS             complement(17603..19948)
                     /locus_tag="MB216_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBT4525268.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20227..21129
                     /locus_tag="MB216_000408"
     CDS             20227..21129
                     /locus_tag="MB216_000408"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBT4525269.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            21342..23117
                     /locus_tag="MB216_000409"
     CDS             21342..23117
                     /locus_tag="MB216_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBT4525270.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23183..24769)
                     /locus_tag="MB216_000410"
     CDS             complement(23183..24769)
                     /locus_tag="MB216_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBT4525271.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFILLLLTKVGFRKIESLVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            25019..25243
                     /locus_tag="MB216_000411"
     CDS             25019..25243
                     /locus_tag="MB216_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBT4525272.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25385..27136
                     /locus_tag="MB216_000412"
     CDS             25385..27136
                     /locus_tag="MB216_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4525273.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            27136..28920
                     /locus_tag="MB216_000413"
     CDS             27136..28920
                     /locus_tag="MB216_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4525274.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            29246..30097
                     /locus_tag="MB216_000414"
     CDS             29246..30097
                     /locus_tag="MB216_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4525275.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            30151..31902
                     /locus_tag="MB216_000415"
     CDS             30151..31902
                     /locus_tag="MB216_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4525276.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            31906..32076
                     /locus_tag="MB216_000416"
     CDS             31906..32076
                     /locus_tag="MB216_000416"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4525277.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(32116..33018)
                     /locus_tag="MB216_000417"
     CDS             complement(32116..33018)
                     /locus_tag="MB216_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4525278.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT        10525 a         5872 c         6977 g         9700 t
ORIGIN      
        1 gattaaagga gatttctaat gaaaaaactt aaattattct taccaataat aggattagct
       61 attcttttat caggctgttc aaagtggatt gatcgaggag aatccattac ggctgttggc
      121 tcttctgctt tacagccttt agtagaaaca gttgctgaaa cttaccagac agaaaatcct
      181 ggaaaattca tcaatgtaca agggggcgga agcggaactg gtttgtctca agtacagtca
      241 ggagcagtcg acatcggaaa ctctgatttg tttgcggaag aaaaatcggg gatcgatgcc
      301 tcagcattag tcgatcatcg agtagcagtt gttgggatca ctcctattgt gaacaaggat
      361 attggcgtaa aagacatcag tatggaaaac ctgaaaaaac tatttacagg cgagatcaaa
      421 aactggagtg aagtcggtgg aaaagatcag ccagtagtta tattgaaccg cgcttcagga
      481 agcggaaccc gtagcacgtt tgaaaaatgg gtattggacg gagaaacagc tgttcaggca
      541 caagaacaag attcaagcgg aatggtgcgc cagatcgttg ccgacacacc cggtgcaatc
      601 agctatgttg ctttttctta tgtttccgat gatgtagata cgttgaatgt taacggtgta
      661 gaaccgacag atgaaaatgt gacaacgaac aaatggacga tttgggcata tgagcatatg
      721 tacacaagag atcatccaaa aaaactgaca aaagaatttt tagactatat cttatctgat
      781 gaagtacaag ataacatcgt tggagaacta ggttatatcc ctgtttctca tatgaaagtc
      841 gaacgtgatt ggcaaggaaa tatcgttaaa gagtaatctt tacacaaaat ttacaaactt
      901 agctaaactt ttacatgtta ttaaaaccaa attgatgttc aatttgtaca ctgagtgagt
      961 atgttaaacc tgattaaggg ggagtcatct gtggaagatg tgaaaaaagt attattaaca
     1021 aaatcaaaac gttcaaaaat ggaacagcgt ggaaaattca tcagtttttt atgtatcgcg
     1081 ctgatcgtgt tggttgtatt atctattttt tattttgtag ctagtaaagg attggccacc
     1141 ttttttactg ataagatcaa tgtatttgat tttctgtttg gtacagattg gaatccaagc
     1201 caaatcggag caaatgggaa accaatggta ggggcattgc ctatgatcac aggttcgttt
     1261 attgtgacct ttttatctgc tatcgttgcc acgccatttg ccattggtgc agcagtattc
     1321 atgacggaga tctctcctaa aaacggaacg aagatcttac agccggtcat cgagctgctt
     1381 gtaggtattc cttctgtcgt ttatggtttt atcggtttat cagttatcgt gccttttgtc
     1441 agaagtattt ttggcgggac tgggtttggt atcttagctg gtacatttgt gttgttcgtt
     1501 atgatcttgc cgactgtcac aacgatgact gttgatgcat taaaggcagt gccacggcac
     1561 tatcgggaag cttcattagc gcttggtgcc acaagatggc aaacaatcta caaagtcgtg
     1621 ctacgagcag ccgtgcccgg gattttaaca gcagtcgttt ttggtatggc tcgtgcgttt
     1681 ggtgaagcct tagctatcca aatggtcatc ggtaatgcag cattgatgcc aacaggtctt
     1741 actactcctg cttctacatt gacgtccatc ttaacaatgg gaatcggaaa tacaatcatg
     1801 ggaacagttg aaaataatgt gttatggtca ttagcgctga tcttgctatt gatgtcatta
     1861 ttctttaata tcgtgatccg tatgattggg aagaaaggag agctgaaata atgaacgcaa
     1921 aacgctcaga taaaattgct acaggtgttt tatatatagt ttcggggatc atcgtattga
     1981 ttttagcagc tttactactt tatatcttag ttcggggaat accgcacatt tcatggtcct
     2041 tcttgaccga accttccaaa gcctaccaag aaggcggtgg aatcgggatc cagctattta
     2101 actcgattta tttgcttttg atcacgatga tcatcagttt tcctatttct ttaggtgcgg
     2161 gtatctatct ttcagaatat gctggaaaaa actggctaac agatgtgatt cgcacgtcga
     2221 ttgaaatctt gagctcgttg ccttctgtcg ttgtcggttt attcggtttc ttggttttcg
     2281 ttattcaatt ccagtatggt ttctcgatta tttctggtgc attggctttg actgtattca
     2341 acttgcctct tttgactaga acagttgagg agtctttaca agcggtccat tacacacaac
     2401 gagaagcagg attggcctta ggtttgtcac gctgggaaac agtgataaaa gtggtggttc
     2461 cagaagcgac accagggatt ttgactggtg tgatcctaag ttctggaaga atttttggtg
     2521 aagcagctgc attgatctat acggctggac aaagtgcacc tgcgttagat tttacgaact
     2581 ggaatccatt aagtgtttca agcccgatca gcattttccg tcaagctgaa acattagctg
     2641 tccatatttg gaagatcaat acagaaggca cgatgcctga tggtgtttct gtttcggctg
     2701 gcgcttctgc cgtgttgatt attgctgttt tactgttcaa cttcggcgct cgtgcgattg
     2761 gcaaacgatt ataccgaaaa atgacttcag cctaaggaga agaagcaaaa tgaaaaaata
     2821 taactggaat gaaacaaatg tcatcaccat gccaaaagag aaagaagtag cactatatac
     2881 ggatgatcta catgtctggt atggcgacaa tgaagcaatc aaaggagtgg atctggaatt
     2941 tgaaaaaaat aagatcactg cactgattgg tccttccggc tgcggaaaat ctacgtatct
     3001 tcgttcgttg aatcgaatga atgatgggat cgctaatacg aaaatcactg ggaaaatcat
     3061 gtatcaaggc gtagatatta atgcgcctca agtggatgtt tatgagatgc gcaaacggat
     3121 tggcatggtt tttcaacgtc ctaatccgtt cagcaagtcg atttatgaca atattacatt
     3181 tgctttgaaa cagcacggcg aaaaaaacaa aaagaaatta gatgaaatcg ttgaaacaag
     3241 cttgaaacaa gctgccttgt gggaccaagt aaaagacagc ttggacaaaa gtgccttggc
     3301 tttatctggc gggcaacagc aacgtttatg tattgctcgt gcgatcgcga tgaaacctga
     3361 tatcttgctg ctagacgagc cggcaagtgc tttggaccct atttcgacag gaacggttga
     3421 agaaacactt gtaaacttga aagaagacta tacgatcatc atcgtgacgc ataacatgca
     3481 acaagcagct cgtatcagcg actatactgc ctttttctat ttaggaaaag taatggaata
     3541 tgacaaaact aacaagatat ttaccagacc aaaaattcag gcaaccgaag attatgtgtc
     3601 cggtcatttt ggttaagacg gaggcctcaa catgacaaaa gagattattt catctaaaga
     3661 tcttcatctg tattatggaa agaaagaagc actaaaaggg atcgatctaa gcttcaatca
     3721 aggggaaatc actgcgatga tcggtccttc tggttgcgga aaatccactt atctgcgttc
     3781 tttgaaccgg atgaatgacc tgattccgaa tgtaacgatc accggaagtg ttttatataa
     3841 agacaaagac atctatggtc caaaaacaga tgttgttgaa ctaagaaaag aaatcggcat
     3901 ggtattccag cagccaaacc catttccttt ttctatctat gaaaatgtca tctatggatt
     3961 gaaattaaaa ggggaaaaag ataaaaaagt ccttgatcaa gtagttgaag aaagcctgaa
     4021 ggcagcgtct gtttgggatg atgtgaaaga caaattgcat aaaagtgctt tatctttatc
     4081 cggtgggcag cagcagcgtg tatgtatcgc acgtgttttg gcagttaatc cggaaatcat
     4141 cttattggat gaaccaacaa gtgcgcttga tcctgtttct actgggaaaa tcgaaagtat
     4201 gctgttagaa ttaagagagc ggtatacgat gatcatcgtg acccataata tgtctcaagc
     4261 ttcacgtatt tcagataaga cggcattctt tttagatggt cacttgatcg aatacaatga
     4321 tacgaagaag atattcatga atccagaaaa acaagagaca gaagattata tttcaggtcg
     4381 atttggataa ggaggaaacg gaatgctacg tacacaattt gaagaagaat tgctgaatct
     4441 ccataatcag ttctacgaaa tggggatgat ggtgagcagc gccgtgcaca agtcagttcg
     4501 tgcatacatc aaacacgata aaatcttagc ccaagaagtt atcgaaaacg atataaatat
     4561 caataacatg gaaacacgct tggagaagaa aagttttgaa atgatcgcat tgcaacagcc
     4621 agttacaact gatttgcgta tgatcattac ggtcatgaaa gctagctcgg atttagaacg
     4681 tatggccgat catgcggtgt ctgttgctaa atccacgatt cgcgtaaaag ggcagacgag
     4741 aataccggaa atcgaaaaag agatctccga tatgtccgat tatgtgaaaa aaatggtgga
     4801 caacgtccta gttgcttatg tcaaaacaga cgaaaaagac gcacgtacga tcgccaatat
     4861 ggatcaaaga gtcaatgact atttcaatcg aatctataat gcaacaatca aaaatatgca
     4921 ggaaaatcct gagacagtca tcagtggaac agactacttg aatgttgcca gttatctaga
     4981 acggatcgga gactatgtga caaacatctg tgaatggatc gtctacttgg ctacagggaa
     5041 aatcactgag ttaaatacga accacaatga gattttctaa actgaagctg tgattatgcc
     5101 gtttgtttag gaaaagaggt tgtgaaatca gcggttgtga aaaagctgtt ctgcatcaag
     5161 taataagaac agtcaaacaa aaaatagctc ctcatatttt tcgtttggct ggacttatta
     5221 ccgcagatgc aagcttttga acaccgttta ttcgggaaag ggtttgtgac aaaacatgtc
     5281 acaaaccctt ttcatcattt catagaaagg ataaagaatg caaaaaaaaa ttagcatcat
     5341 cactatattt tttatttgtg tgcttacttc atgggttatt cagcatcaag ccgctttata
     5401 tgaagtacct gtcatcgaga tagaaaaagt acgaataaaa gaggatcatc aattgattac
     5461 aggaaaactg atgaacacgg atcaaaaagg agaatcgatt tatttatcgg ttccttatca
     5521 aaaaaatcaa atagaaacag caaatataaa agcgagacaa atgtatctag tccaaatgca
     5581 tggagatgaa tggcagctag tagcaaaaaa aaatgatggc tggcttttct tcttcacaag
     5641 tatcttcatt ggcgccatgt tgcttattgg tggaaaacag ggtctatttg ctttaggaag
     5701 tctcatcatc aatatcgttt tacttttact tttgttgctc atctttacta aaacagaagg
     5761 gatttctcta ttaaatatat ctatcctgtt cgtcgttata gggatgatta ttagcatatt
     5821 ggctttagat ggatggaata gaagcagtat aatcaaaatt tcagctgcag ttacgagtgt
     5881 ttttttagca ttttttattt gtcttttgac aatgaatcat tggaaagacc aaggactgcg
     5941 ctatgaggaa ctgaattatc tgacacgacc gtatcgttct gtttttcttt ctagcgtact
     6001 gattggaaca gctggaggaa cactcgatac cgttatcaca gtcattgcga cattagaaga
     6061 attgactaag cgacaaccag aaataacagc gaaacagtta tggctttcag gaaggcaagt
     6121 gggtcgagat gtgattggct cgatgaccaa tatccttttg ttcgtctata tcagtgggtc
     6181 agttccatcg cttcttttat atttaggaaa tcaatggtcg ttcaaagaaa cgatagaaca
     6241 gcatttatca ttggaatttt tacgcgtcat tgctggcagc ttaggaatag tattatcgat
     6301 ccctatctcg gtcctctttt ttctgactgt aaggaggctg aaacgatgaa tgtacttatt
     6361 atacttgttg ggattttagc aatcagcgtg ctacttattg gcggtacgca aggtatgtat
     6421 attcttttag gcttattcat caatttaggg atctttttcc tgttgttatt cggttatcat
     6481 cagaagtggc cgatactggc attgtcggtc ataggatttt tattgattgc tgtggttatc
     6541 ttatttttta tcaacggtta taatctaaaa atgcgtgcag cttttgtgag tatactgatc
     6601 tttttatttt gtttcctact attgcttcct atcactgatt ttttagcaat ccaaggtttt
     6661 acatcggtag aactagaaga attatctggc ttagataaga ctttagcgat tgattttaga
     6721 ttacttacga gatcgttgct tctgattagt cttagtggtg ctgttttaga tgcgagtgta
     6781 gcaatttcta gtgggacatt cgaagtatat caggcaaatc cgcaattatc tttttaccaa
     6841 ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg cgtcaacagt aatgacactt
     6901 ctttttgcat tcatgggaag ttctttagcg ttgatcctct ggtttattga tttagacatt
     6961 ccttttcagc aagtgatcaa tgaaaaatca tttgttctag aatatacgat ggctattctt
     7021 accacattgt ccgcactact tgttcttcct ttaacctgcg tgactacagc atatctcttt
     7081 acaaaaaaga aagaacaact caagatggag taaaagttac aacttaagtc ctatgacatt
     7141 tttactgaat cagtgcataa tatggatgta aggaaaagct tgcaaacaat tttgcttaag
     7201 gaggcaattt ccatgaaaga aagagaacgt attttagatt tagtgaaaaa aggaatcctt
     7261 tcgactgaag aaggcttaga tttattagaa agcatggcaa ctgaaaaaga cgaaaagcaa
     7321 atcaaaaaag aagcagacaa ggtaactgct agtcataaag aaaaagatca ggcaagtcaa
     7381 ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg agccagttga tccaaaggaa
     7441 agagaaagac aagatcaaga aaacttagaa aagatcttag atgaactagc aactgaagcg
     7501 aataaaactt cggcgcgtct agatgaagta aatgctcaat tcgctgacaa taaggcagca
     7561 cgcaatgaga aacaggaagc attgatgcag ctgaatacaa aagaagaatt aggcgagttg
     7621 acagaagaag aattagcaca gcgtcagacg ttagaagcag aaatcaaaga attagaagct
     7681 agtggcgata cactgttaga agaacaattg aaattagaag cagaattaaa agatatccgt
     7741 aagaatcaat ggtctgagaa aaaagaaacg tttactgata agtttgaatt gccagatgat
     7801 tggaaagatc aagcaacaga tacactgaat caagtaggcg aaaaaatgtc agaagctgga
     7861 tcacagttag gcaaattctt gaagaaaaca ttccaaactg tttcagaaac agtgaatgac
     7921 aacatggaat ggaaagatgt cagcttacgt gtccctggga ttgcgacaac gaaatttgag
     7981 catgaatttt actatgaagc tcctgcagca agtatccttg atatcaaggc agcaaacgga
     8041 aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag ttgaagctaa gatcaaactt
     8101 tacggaaaaa tgggtgctga accatttgaa gcattctctg aacggagcca gattgaggtt
     8161 aatgaagatc atatctcctt ccaaattcca aataaacgtg tgcgtgcaga ccttgttttc
     8221 tacttaccta aacgagtata cgaccacgca gctattaaac ttctaaatgg caacatcatg
     8281 attgaaacat tggaagctaa agatatttat acaaaatcta cgaatggtaa catcatcgtg
     8341 gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa acggcaatat cgatattcga
     8401 aacgggaaca tcttagattc tattatcgaa acagtaaatg gtacagtgac atttggtgcg
     8461 acagcagaaa acctttctgt gtctttggta aatggtgatg tacgtcttac gattaaagaa
     8521 gataatttga aaaaagtaga agcaagttca gtgaacggaa atgtcaaagt cgctttacca
     8581 gatggcatcg gtttggaagg acatgctaaa acaagtcttg gaagtatcaa cagccgtctg
     8641 tccaattacg aagtagttcg tgagaaaaaa gaacggacaa accaaatgtt gcaattccgt
     8701 cgtgtttcag atggtgaaat cgcgcaagtg caactttcaa cgacaacagg aagtatctat
     8761 ttaaaagata cagataaata aagaggttgt gaaagagatg ttcagccttg agcaataaga
     8821 agcgattttg taaaatagct tcccctattt tacaaaattg tgccttattg ccgatagtct
     8881 atctcttgaa caccgtttac caagagatta tgaacctgcc aattatcgcc gagtcacaag
     8941 gaataatttc agaaaaagct cgtcacgttt tatgaatatg gtaatttgtg accgagaatt
     9001 ttgcagaaag acaccgatta agaagaaggt tgtcacaacg attttcttca tttaaaatag
     9061 tagaacagag gtgagacatg atgaaaaaaa agttgacaaa atctaataaa aatgttgttt
     9121 tgacagggac tttagcagga ataggagaat acatcgggat tgatccgact gttatccgtg
     9181 tgctttatgt attcctgagc ttagttgctt ttggaagtcc aattatttta tacattttat
     9241 tagctttgat tatcccatct gcgcgttctg cacgaacaaa ctatggacat aacaatgcat
     9301 attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca aacaaaaaaa cgcaaagaag
     9361 cggaaaaaat cgatgatgac gaatggagtg acttttaatg tcgtactttc aacgtatttt
     9421 agttaatacg ttaacattca tatcattggc tgttcttttt cctgcgatgg tccatgtgag
     9481 cagtatttgg acggccgttc ttgccagctt cgtattgtct gtgttgaata tgtttgtcaa
     9541 accgattttg acgatactgt ccctgccctt tacattagtc acattgggat tgtttacgtt
     9601 tgttatcaat gcagcgattc ttaaaatgac ctcctttttt gtgggagcag aacattttag
     9661 tttttcttca ttcggtgcag cgttgttagt agctatcttg atgtcgcttg tcaatgtaat
     9721 tgtcagcgaa cataacagta gaaaataaaa gaaagctgtg ccgaactatc ggcgcagttt
     9781 ttttatttag aatcaggaat gcgttttcta ttacattggt aattaaataa aaacgttttt
     9841 tttaattggt agtacttttg gctagacaat gataaaatga tagagaacac tctaatagaa
     9901 atgaggtagc atgaatgtca gaagttgtaa aagttagcca attagtgaaa aaattatctt
     9961 tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt catcgtaact ggggacatct
    10021 cacgcccagg actagagttg actggttatt ttaattatta ttcccataat cgtttacagc
    10081 tatttggcag taaagaaatc agttttgcgg aacggatgat cccagaagag cgtttgatca
    10141 tcatgagaag attatgtagt gaagatatgc ccgcatttat tatttcaaga ggattaccag
    10201 ctccagaaga aatgatccag gcagcaaatg aaaatgggat agctgtactg cgttctccca
    10261 tctcaacttc tcgtttatta ggagaattat ccagttattt agatggtcgc cttgcccctc
    10321 gaacaagtgt ccacggtgtt ttagtggatg tttacggatt aggtgtactg attcaaggtg
    10381 acagtgggat aggaaaaagt gaaacagcgt tggagttgat caaacgcggc catcgattga
    10441 tcgcagacga tcgagtagat gtttatcagc aagatgaatt aacggtgatc ggagaaccgc
    10501 ctaaaatcct ggaacatctg attgaaatcc gaggaatcgg gatcattgat gtaatgaatc
    10561 tttttggtgc gagtgcggta cgtggctcta tgcaagtcca gttggctgtt tatctggaag
    10621 catgggcaaa agataagaaa tacgaccgtc tgggctctga agacacgacc gttgaaatcg
    10681 cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac aggacgaaac gttgcaatca
    10741 tcattgaagt agccgcgatg aatttccgtg cacgtacaat gggctttgac gcaacgaaaa
    10801 catttgaaga aagattaagt cgcttgattg aagaaaactc aggcaacgac tagcaaggag
    10861 tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag cttgtaggga ttcctatcta
    10921 ctggtatgca ttgatcatcg tatcaggtat cattatcgct atgtggctta gcagcagaga
    10981 ggctgttcgg gtaggattaa aagcagatga tgtgacagat tttatgttgt ggggattgcc
    11041 tatctccatt attggcgctc gcttatatta tgttttattt gatcttcccc agtatatagc
    11101 tgatccaatc caaatattca atatccgttc aggaggctta gctatttatg gcggactgat
    11161 tgcaggcgcg atcacgctgt atttttttac taaatatcat tttatttctt tgtggacttt
    11221 tttagatatc gctgcaccaa gtgtattatt agcacaggca atcggtcgtt ggggaaattt
    11281 catgaaccac gaagcatatg gaccagacac gacgcgaagc ttcttagaaa gtctgcattt
    11341 accgcaattc atcattgata atatgtatat cgaagggatc tatcgtcaac caacatttct
    11401 ttatgaatca ctttggagtt tgattggatt tattttgctt ttgtttttac gaaagaaacc
    11461 acaccttttg aaaaaaggcg aagtcctttt atgttatcta atgtggtata gtttcgggcg
    11521 tttctttatt gaaggattac gtacagacag tctttacttt ttaggtgtga tccgtatttc
    11581 tcaattatta tctgctgtgt tatttgtcgg tacattgatt ctatttattt ggcgcagaaa
    11641 aaaaggaaat ttaccggatt atgaccgctc attaggaaaa aatcaacaaa ttatctgatt
    11701 tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa aatgaattat agagtagcgg
    11761 ttttaggacc aggctcttgg ggtactgctt tggcacaagt cctcgccgaa aacggacatg
    11821 acgttcggat ttggggacat cgtgcttcac aagtagaaga aatcaacacc caacacacga
    11881 atcaacgtta tttaccagat cattatctac ccgattccat ccaagcattc accaatatgg
    11941 aagatgcagt aaaagacgca gatgcggtgc tattcgtcat tccaacgaaa gcaatacgtt
    12001 ctgttgcaaa agaactagtg cctaaattga atacaaagcc agtgatcatc catgcaagta
    12061 aaggattaga acagggaaca cataaaagga tctctgacat cttgttagag gaaatcccag
    12121 cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag ccatgcagaa gaagtagcaa
    12181 aacatgacat cactacgatc acagctgcaa gtacagatgc aaaagcagca aaatatgtac
    12241 aagagctgtt catgaataat tattttcgga tctataccaa tcctgatgtg atcggtgtag
    12301 agatgggggc agctttgaaa aatattattg ctattggtgc tggcgctatt cacggcttag
    12361 ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg attagctgag atcagccgtt
    12421 taggtgtagc aatgggtgcc aacccgctga cctttatcgg attaagcgga ataggcgatc
    12481 tagtcgttac ttgcacaagt gttcattcta ggaactggcg cgcaggcaac ttgttaggac
    12541 aagggcagcc cctagaagag gtgctagcca atatgggaat ggtcgttgaa ggtgtgaata
    12601 ctacccaagc agcgatggaa ctagcccaaa tgatggaaat cgaaatgccc atcactcaaa
    12661 caatctatga agtgctgtat gaacaaaaag aaatcaaaac agccgcaaaa gaaatcatgt
    12721 tacgtgatgg aaaaaaagaa aatgaattct cattaacaat tttttgaagg agcgaatgta
    12781 tatgaaagtt aaaaaagcag ttattccagc agcaggatta ggaacaagat ttttaccagc
    12841 aacaaaagct atggcaaagg aaatgttgcc aattgttgat aaaccaacaa tccagttcat
    12901 cgttgaagaa gcgttgaaat caggtatcga agacatcttg atcgttacag gaaaagctaa
    12961 acgaccaatc gaagatcact ttgattcaaa tattgaatta gaaacaaacc taagtgaaaa
    13021 aggaaagacc gaattattga aattagtaga agaaacgaca gatgtcaact tgcatttcat
    13081 ccgtcaatct catccaaagg gtttaggaca tgccgttctt caagcaagag cctttattgg
    13141 aaatgaacca tttgtcgtta tgctaggcga tgatataatg gaagacgaag taccattgac
    13201 acagcaatta atcgatgatt acgaacgtac tcatgcttct acgatcgccg ttatgcaagt
    13261 tcctcatgat gaaacttcaa aatacggaat tatcaatcca ggagaagtat tggaagatgg
    13321 gttatacaat gtcaaaaact ttgttgaaaa accagatcca agcaaagcac caagtgattt
    13381 agctattatt ggacgttact tattgactcc agaaattttt gatgtattgt ccgagcaaca
    13441 accaggagcc ggaaatgaaa tccaactaac tgacgcaatc gatacattga ataaaacaca
    13501 acgcgtattt gcccgtaaat tcacaggtaa acgttacgat gtcggtgaca aatttggttt
    13561 tatgaaaaca agtattgaat atggtctaac acatccagaa gtaggtgcac ctttacgtga
    13621 gtacatcctg tctttaggta aaacattaga agcagaagga agcaagatcg atcataatcc
    13681 aaaaggaata acagaaatca aagaagaaaa gaaaaaataa cccttttttc taacgagctt
    13741 cagctgctaa ttgaataaaa agtagttgaa gctcgttttt atttgtttaa aaaacacgaa
    13801 aatgctatcc ttatagaaaa gagagacaag aagggagttt gatcatgtca ggaggagaaa
    13861 ttgcaggatt gatcgcagca atcgcctttg cgatacttgt cgtttttatc gttcgtgtgt
    13921 tgttgcaagt ttctaaaact ttagaaaaag tagatcagac tgtagcagaa gcaaatacga
    13981 cgatcgaaat cgtcacaaaa gacgtagatc ttttgtctag acaagtagaa ggcttactgg
    14041 tcaaaagtaa tgaactgctc ttggatatca atggaaaagt cgcaacgatc gatccattgt
    14101 tcacagcagt tgctgattta agtgaaagtg tatcagaact taatcattct agtaaaaaca
    14161 tcgcgacgaa agtcggcgga atcggtaaaa cgacagctaa agcgacggtt gccggaaaag
    14221 tcggtgggac agcgatgaaa ttttttaaaa acaaaaaaca tactgaaacg acaggaggaa
    14281 aataaaaatg gctaaaaaag gtggattttt attaggtgca gtagtcggag gcacagccgc
    14341 agcaatcgcc gcgttattgt tagcaccaaa atctggtaaa gaacttcggg atgaattagc
    14401 tgcccaagcg gatgatttga tggatatggc ttcagattac acagatatgg cgaaagaaaa
    14461 atcgactgag ttaacaggta ttgcaaaaga aaaagcgcaa gatctttctc aacaagcagg
    14521 tgatctagca ggtagtgtca aacaaaaagt aagtgaatca gcagatgaag caggaagtga
    14581 atctgacgat atcttgttaa atcttaaaga acaatcagct gacttatcta atcgtttcaa
    14641 aaaaacggca gaagatatga aaaaacctgc agatgaatta ggaagtattg ctaaagatac
    14701 ttcagaagat atcatgattg atgtaaaaga cgctgctggt aaagcaggag atactgtcaa
    14761 tgaaggaatg tcagaagcaa aaccagttgc agatgactca aaagaaatga tgaaaatggc
    14821 aaaagaagat gcgaaagaag ctaaaaaaga aataaaagat acaatgacag attcatctac
    14881 aaatcactaa aatcacaaat ttatagaata aaaaggtggg agcggactag ctctcacctt
    14941 ttttactctt atttttctaa aacaagtaat tcttttggtg tagtcgtgaa tggcacacag
    15001 ccatcgcttg tcacatatac acagtcttcg atacgtaccc ctactttttc aggaatatag
    15061 attcctggtt caagagagaa gcacatccct tcttcgatta ctaagtcatt tccttctaca
    15121 agggaaggga attcgtggac ggttgttccg atcccgtgac ctaaacggtg attgaaatat
    15181 tcgccgtagc catagctgtt gatcacgttt cgtgcgattt gatcaagttc acctgctgtc
    15241 acacctggac ggacggcttc ttgtgcggca agctgtgctt caagagtgat gttgtagatt
    15301 ttttcttgga agtcggttgg tttttgataa gcaattgtac gagtagcatc actgcaatag
    15361 ccattccaaa caacgccaag atcgaataac acaagttcat tcggtgaaat cttcgtattt
    15421 cctggtgtac catgagggct tgcagcattt gctcctgcaa ggactaatgt gtcgaaagac
    15481 atttgtgaaa caccttgttt tttcaattgg tattcaattt ctgcaatgat cgcttgttca
    15541 gtcacgcctt ctttcactgc tttaaagcca atctcaaatg caacatcggc ccatttacca
    15601 gcttcgatca gtttgtcttt ttcctctaga gttttgatca gttgtagacg ttggatgatg
    15661 gaagttagat cagtgtaaga atccgttccc gtcaagatat cagagatagc atcgaagcgc
    15721 tcgacagtca aggcgttctt ttcaatccct acattgccag gctgaccata gcgagcacga
    15781 agttcttttc cgatgatctc ccaagggttt tcgctgtcta aatagccacg gacatcatac
    15841 gcccaactac tgttttgagc atcttcaact tccaaagcag gagtgaaaag gaaaggatct
    15901 tgttcaagag ggataaaaag agctagtaca cgctcatgag gatcactttc gtatccggaa
    15961 aagtaagcga tgtggccagg gtcgctgata taagccaatt cgacttggtg ttgtgccatc
    16021 cattgacgta gttcattgat ttttacgtta ttcatttggc aacctacttt ctaaattaat
    16081 tttgtattca ataattgtac cactgattta ttaaaagacc aacgttctaa aaactgaaaa
    16141 aagtaaaaat tttcatgaaa acagagataa aacggttgca attcttgaga ttatttgata
    16201 ttctatttta gaaatgtaag acgtattttc agaaaacgta taaaatatga ataggagaac
    16261 acaaatggaa aagcaaacaa tcactatcta tgatgtagcg agagaggcaa atgtttcgat
    16321 ggctactgtt tcacgtgtcg taaatggcaa tccaaacgtt aaaccagcaa caagaaaaaa
    16381 agtcttggaa gttatcgatc gcttggatta tcgtccaaac gcagttgcgc gtggcttggc
    16441 aagtaagaaa acaacaacag ttggtgtaat catccctgat gtcagcaata tgttctttgc
    16501 ttctttagca cgtggtatcg atgatgtggc aacaatgtac aaatacaata ttatcttagc
    16561 aaattcagat ggcaacgacc aaaaagaagt caatgtattg aataacttac tggctaagca
    16621 agtggacggc gtgatcttca tgggtcacca tatcacagac gaaatccgtg gagaattttc
    16681 acgttcaaaa actcctgttg tattagctgg ctctattgat ccagatgaac aagtaggtag
    16741 cgtaaacatt gattatgtat ccgcaacaaa agatgcagta aacaaattgg caaaaagcgg
    16801 caataaaaaa atcgcttttg tcagtggtgc gttgatcgat cccatcaatg gacaaaatcg
    16861 tctgaaaggc tataaagaag cattaagcga aaataaccta ccatatagcg aaggattgat
    16921 ctttgaagca caatacaact tcaaagatgg tctatctcta gtcgatcgga tccataatag
    16981 cggtgcaaca gctgtctatg tttcagatga tgaattagcc atcggtattt tggatggttt
    17041 attagaccgt ggcgtgaaag taccggaaga attcgaaatc atcacaagta acaattcact
    17101 actgacagaa gtagctcgtc ctcgtttaac aagtatcaca caaccattat acgatttagg
    17161 ggcggtatct atgcgcctat tgacgaaaat gatgaataaa gaagaagtcg aagaaaaaac
    17221 gattatcttg ccttatggaa tcgaagaaaa aggatctaca aaataataaa aggacaacca
    17281 ttcagaggtt gtgtgcctgc cgtttaactc tgcgttacaa ggagcaattt caaaaaacag
    17341 catctcatgt ttgatgaata ttgtgactcg tgattcggag ttggcaggag aacaccgttt
    17401 atttgggaaa aggggttatg acaaaatttt gttataatcc tttttttgtg tcttaaaaaa
    17461 gagagaaagt ggttgaaatt acatttatcg tatataaaac aatccgtgta tagatgctgc
    17521 attttgttca ccaccatcaa aaaaataagg gactgtgaca atactattgt cacaatccct
    17581 tattactgtg tattctatta aatcaatcat ctttctcatc tgattcttta gttgcttgat
    17641 tcgctcttgc ataagctgaa gcggtgttcc agtaatctgt atagttttca tcggtaccgc
    17701 caacgccaaa tttaaaggcg cttttcttcg gtccattttt cgcccatagc gattcaactg
    17761 tttttcctgg tgtttggatg gattttttat tgacagtaac ttttccaggt gtttgaccag
    17821 taaatccaga tacttttttc ttcatcacat cagatgataa ttcgaatttc tcatcagtgc
    17881 caaaaatgtc aggttttgct tggtaagctt gattgatcag attggcaaga taattagccg
    17941 aacgtatcct tgctttgctg tccattccag tattgtcatc gtgccctgcc cagctactga
    18001 tggtaataga cggagtagag acaattagcc atgaatctcg gtattcattg gtagaaccag
    18061 tttttccgac ccaatctgct tttcctagat ttccgtttaa gcttgagatg gcatctttga
    18121 atggtgtagt gatttttgca ttgatcacac tacgcatcat gtcattcatg atcgatgccg
    18181 ttgcttccga atagatccgg acagagttgc tttcgtgttt ataaattaca tttcctgcgt
    18241 tatctgtgat ggaatcgatg atatagcctt cttcatagac gccgccgttt gctaaagcct
    18301 gaaatccgtt ggtctgttga agcgtcgtga catctactgt acctaaagga gcagattcaa
    18361 cgccccaatt gtcatttgcc gggtagttca tctttgccaa gtagtgctcg taagcatatt
    18421 gtttcgatcc accggaattc aacacatctt gatataagtg ataagccggg atattgttgg
    18481 accattctag tgactcacgg acggtttgga aggtgttcga tccttcgtta gtagcattga
    18541 cgattttctc gccagcattt tctccttctt gccaagttgt tgcataatct gatacacggg
    18601 attctgatcc gataagcccc atgtcgattg caggtccata aacaagaact ggtttgattg
    18661 atgagccagc ttgacgttct gtatcgaagg catggttatt ttgattctga ctgtaattcc
    18721 gtccgccaac gaatccgtag atgcggccag ttctgttatc catcaataca tttccggttt
    18781 ctacttgact gccagcacca tcatctaaca tgtatccata attttcaacg ccattttgca
    18841 atgcagcata gacatcttta tcgattgtcg aatggattgt atatccttta ttttggatcg
    18901 tttgctgtgc cttttgataa taagcgtcac tgaccgatga atcagacata tcagcgttgt
    18961 ctttttcagc caattgttgt gcaatgattc ttgttgcttc attcatcaca gtataataca
    19021 agaattctct gtcagtctgt tctgcgattt gttgtggtag aaagtctttt gtaaggtcat
    19081 aggcttttgc ttcttcgtat tgttctttcg taatttggcc ttcacgatac atactgaata
    19141 aaacaaaatc ttttctggca aggccggcag aaagatcttc ttttagcgcg cctgtgttcg
    19201 tgtatggact atacgtgatc gggctttggg gtaatccagc gatataagca gcttgcggca
    19261 gcgtcacatc ttttgcatct acaccaaaga ttccttttgc agcttcctgg actccagcaa
    19321 tgttttgtcc cttattatta cgaccaaatg gtgagacatt caaatacgta gcaatgatct
    19381 catcttttga aaagtatttt tctacttgtg cagaaagaat gatctcgttt gctttacgtt
    19441 tgaatgttgt ttcatctgta aggatctgct gtttgactaa ttgctgtgtc aaagttgatc
    19501 caccagaaga gccaatacca gtagcttcag atactaatgc tcgtaaaaca gctttaggaa
    19561 cgtaaccttg gtgtttatcg aagtattcat cctctgtgct gataattgca gttttcaata
    19621 aaggtgaaat cttatcagat gaaatagtcg tgcgcatcag atctgtctgg atttttgata
    19681 tctctgtatt atctgcataa acaatctttg acgtttctgt aatgtttcct agttctgtct
    19741 gcagtgcttt tttagtcggc aattgtgtgt cttcgacgag ataggcaaaa tagccagcgc
    19801 ctattccaat acctagagct gcgaacatca aaagaagaac agtaataaaa acagtcaatg
    19861 attgaaaaac tctaaaaata atcttaggaa caaaccattt ttcttttttt cttctattct
    19921 gtttttttcg agtatttttg cgtggcaaaa gggtcacctc aactaatggt tttatttcgc
    19981 taattatagc aaataataga acaaaaaaac agacaaggct gtttttggca ggaatttctt
    20041 ttttaaaaga gttaaaacgt gagaaaatga tctttattat aaaagagttc ttaagctttt
    20101 ctttagagaa aatttcagtt actatggaga acagggcaaa gaaaaatcaa tcattttctt
    20161 tgtatatcat cttaccaata tataggaatt gttagtaaca attgcctttc aaggaggaat
    20221 ccatccatgt tattaggctc acacgtcagc atgagcggaa agaaaatgct gttaggagca
    20281 gcagaagaag cagccagtta tggatcttcg acttttatga tctatacagg cgcccctcaa
    20341 aacactagaa gaaaagccat cgaagagatg aatattgatg ccggaaaagc gtacatggca
    20401 gaaaaaggtc taacaaatat cgtcgttcat gccccttata tcattaattt ggggaatacg
    20461 atcaaaccag aaatgtttcc ttttgccgtc caatttctga gagaagagat tgttcgtgcg
    20521 gaagctttag gcgctactca aattacactt cacccaggtg ctcatgtagg ggcaggaaca
    20581 gaagccggaa ttgctagaat tattgaaggc ttgaatgaag ttttgacaaa agaacagacg
    20641 gcacaaatcg ctttggagac aatggcaggt aaaggtacgg aaatcggtcg aacatttgag
    20701 gaattagcgc aaatcatcga tggagtaaaa ttgaatgaaa aattatccgt tacttttgac
    20761 acctgtcata cgaatgatgc cggttataat attaaagaag acttttcagg tgtaatggaa
    20821 gagtttgata agatcatcgg gttagatcgg cttaaagtac tccatatcaa tgattctaaa
    20881 aatccgcaag gttcgcataa agaccggcac gccaatattg gttttggaac aatcggattc
    20941 gacacgctga ataagatcgt ccatgatcca atgtttgaca atatttcgaa aatcttggaa
    21001 actccctatg taggcaaaga taaaaagaaa gcttacgcac catatggaaa agaaatcgcc
    21061 atgttcaaag cgcaaacatt cgatccagaa gtgtttcctg agttattagc agaagaagtg
    21121 atactctaag attttctatc tgacagcagc aaaaaaggac caggcacatt gccttgtcct
    21181 tttttgttta agaaacaaag gagtactttt tgcagtcttt ttcatcatgc atcttctccc
    21241 ccctcttttt aaatttagac aaacaaggta tactggtaga aaagtaaaaa aacgaaaagt
    21301 ttcacaagca agtggcaaaa cgtgattgga aggacgaaaa gatgctgaac actttaaaac
    21361 ccttgatcaa ccaactaaaa aagaaacata catactttat tcttggcata gccctattat
    21421 ttacaattat tcgtttgtat ttatatagta aagcaatcta tggaacagac atgtcggcaa
    21481 cctatgatga ccagctgctg attcgctatg ctcaaaatat tttggacggt aaatggctag
    21541 gggagtatac gactacaaca ctttccaaag gaatctccta cagcttattt ttagtacttg
    21601 cacataaact gcatctttct tatgcagttt tgttcggcat gttcagtatt tttgccagta
    21661 tattggttgc actcgcgttt aaaccgattg tgaagaacaa gtatcttctt ttaagtatct
    21721 atttttattt tttgttttct ccgattagtt ttacacatga atattccact cgaacatatc
    21781 gtaatgccct cgtgattccg gctgttgaga tcattatcgc ttgtttatta gctatttatt
    21841 accgcaaata cggttctagc agaaaattgc tgccatggtt tttagggctt tctgtcgctt
    21901 ttcctttctt ttggttcatt cgcgaagatt ctatctggct tcttcctttt actgtagtag
    21961 ctttgggtat cagtttcgtc cagatcttgc tagggcaaga cttttctttc aaagaagtaa
    22021 agaagttaaa aaaacgactc tttcttctct cgaaacggga atggctgaaa ctttcattgt
    22081 ttttacttcc tatagttggc ttttccatcg tctatcaatg gatcgaatat aaaaatgaga
    22141 caacatacgg tattgcaacc atcaatgacc gttccagtgg agaatttgga cgtttgatga
    22201 aacaactgat ccgaatagat gatgaaacga acttgaataa agaaaacagt gagatatggg
    22261 tctcacatgc tgcactaaat aaagcattag ccgcatctcc tacattccat accgtttcta
    22321 cccgtattga cgaattatat acgactcacg cgtggactca aggtggaaaa gtaaaagaat
    22381 tacccggaga tatcatcttt tgggcattgc gagatgtggt aagcgaatct ggttattacc
    22441 aaaacaatgc aaaagaaacc aatgattttt ggaaaaaagt caaccaagaa ttagaagaag
    22501 cctatcacaa gggaacaatc aaaaagaaaa aagagttcta tctgatggga tcaggagatg
    22561 gaaagacgat gagtgatctg ccaatcgtat tcgatttcct tcaatcagga tacgcttatg
    22621 catggcgata taaagatttc catcaaggag gaaattatag tttcggaacg caagaacaag
    22681 tcaaacaggc tcaagaatta ttaaaccttc cgctaatgga gaattggatc gatagtaatg
    22741 aaccgagaac acctaatttc caattaacaa aatcagcaaa agtcgcgaat gtcatcataa
    22801 aaatttacca agttctggct attcccgtgc tgattgcttc aatagcaggg atattactta
    22861 tgatactagg aaccgtattt gcaaaagctg atcgtgccta ttatagttcc tctttgattg
    22921 tggtcaccgg attaatactg acacaattgg tttttctttt cggtgtctca tggttttgtt
    22981 cttatgcgcc agaaaaaaag gactattttt taagtgtcta cactggtgca ggcgtgccat
    23041 taacgcaatt catcgtagta acaagcatgc tagttttcag taaattaaat ctgaaaaaag
    23101 ctttatcttc taaataaaaa gacccttggt agctagattt ttaacctaac tatcaagggt
    23161 ctttgtgtgg aaaaatcagt ttttattcga tttcttctac cgtttcctgc aaaagctgtt
    23221 catatttttt gtttccttta tataaatcac caatcgtcca tgccaacagg agaaggacaa
    23281 gaacaatgat tacataagcg atgtgatcag ccaatgctag ttgagcggaa gtggcttgct
    23341 cgccgaagaa actttcgatc tgtcctggta atcctcgtaa gttcaagtaa gttagactaa
    23401 tcacagagaa ccagccaaga agttttacga tgaatgaatt tttaaatcgg ctacccatct
    23461 ccgcttggct gttggtcatc atcagtaaag gaatcatcga aaacggcaac gcaaaagcca
    23521 agaacacctg ggattcattc attaagttat tcaaagcggc atgttcttcg atcgttcctt
    23581 ttgtacttgt aaacaaaaca caaatcaata caggaatcac agaaagcaat cgtgtgacca
    23641 gtcttcttag ccaaatcggg atacgcatat ggatgaatcc ttccatgatg acttgaccgg
    23701 tcaacgtacc agtaatagta gagttctggc cagaagcaag aagtgctaca gcaaataaag
    23761 tggacaagat accagatttt gctacagcaa tcaaaatacc attgctcata gctgatggat
    23821 cagataaagc ttcatacaga ccaaaaaagg aaggatcttg gacagaaccg gttttgaaga
    23881 cagcgacacc catgatcaat agcagtgcat tgactagaaa agccattgac aactggatat
    23941 tagaatccca agtagaaaaa cgtacagctt gtgctacatc ttcttcgttt tggtgatcga
    24001 tcttccgagt ttgtgaaact gctgaatgaa gatataagtt atgcggcatg actgttgcac
    24061 caataatccc taaagctcca gtcagcggag tttcgccgcc gatgaccggt gagtctgaga
    24121 atgtatgact gttagggata aagccagcaa ataaactgtg ccaatctggg ttagacaagc
    24181 caacttgata gacaaaaacg aataaaatga cgaaaattaa gcagacaacc agtgattcga
    24241 tcttacgaaa cccgactttt gtcagtagta agagaatgaa aacatctaat acagtaataa
    24301 agacggcaat caccaatgga atatcaaaaa gtaagtagag ggcgattgct gcaccaatga
    24361 cttctgcaat atctgttgcc ataatggcta attcagtcaa gatccataaa atgacaccta
    24421 atgtcttact tgtgcgtgct cggattgcct gtgccaaatc catttggctc acgattccta
    24481 attttgctgc catatactgt aaaagcatag caatcagact agacatcaaa ataactgaca
    24541 tcaataaata ttggaaattt tgtcctccgg tgatcgaagt agaccagtta cccgggtcca
    24601 tatacccaac tgctactaac gcaccaggtc cagagtaagc aagaagtgtt ttaaaaaaac
    24661 ttttcccctt tggtacatca actgttccat ttacttcagc taaagataaa ccatttgtat
    24721 gttcaataag tcgatgctta tgaaattgtt tattggattg attattcaag aaaaaaccac
    24781 ctttcctatt tatctacgat tacattctac acctcaaaaa gaaaaaatta aagaaaaaga
    24841 ttcggtacgc ctaatttttt tcatggcgcg tcttgctaga tgacgaaaga aattttgttc
    24901 tcctctaaaa aaaccactat tttttgcatg aaactaaaga atacgccttt cttttttcaa
    24961 taaactttag taaaataagt cattgagagc aattcggaaa tagaaaggag agatttatat
    25021 gttatcaaca ggtattgtcg tacttattgc tattattgct ttgctaatcg gcgcagtagg
    25081 tggctttttc ctagcgcgta aatatatgaa agactattta gaaaaaaatc ctcccgttaa
    25141 tgaggacatg ttgcgatcaa tgatgatgtc aatggggcaa aaaccatctg agaagaaaat
    25201 tcgccagatg atgcagcaaa tgaagaacca agggaaaaaa taggttctga cccaacgact
    25261 ttttacgggc tgattttttc agtccgtttt ttatttcgta atttgttaga ctattcagat
    25321 tattcagcaa aaaagacaag cacagacaat cctaggctct tgataaagac aaaggaggag
    25381 taaaatgtcg atttttagaa aattaggctg gttttttcgt caggaaaaga aacattattt
    25441 aattggggtt ttctcgctat tcgcagtggc cttggtacag ttagttcctc caaaagtgat
    25501 tgggatcatc attgatgaga tcgccgataa aaatttatca ataaagatca ttttcgggtg
    25561 gatcttccta ttgattatcg ctgctttttt acaatatctt tttcgttata tctggcggac
    25621 gaatatttgg ggaagtgcag ctcgattgga gaaagaactt cggcagcagt tattcaaaca
    25681 ttttacagaa atggacaatc tcttttacca aaaatatcgg actggggatt tgatggctca
    25741 cgcaacgaat gatttgaatg cgatccagaa tgtagcagga gctggtatct tgacctttgc
    25801 agattcagtg attacgggcg gaacaacgat cattgcgatg attttgttta ttgattggcg
    25861 cttaacactg attgcgctga ttcctctgcc tttgctggcc gtcacttcac gagtgctggg
    25921 gtcaaaactc catgatgctt ttcgtgattc gcaagaagct ttttctagta tcaatgataa
    25981 gacacaagaa agtatcactg ggatcaaagt cattaaaaca tttggacaag aaaaagaaga
    26041 tctagcggat tttgaagaaa agattgaaga tgcaattatt aaaaacaaac gagtcaattt
    26101 tttagactcc ttatttgatc cattcattac cttgattatc ggtctgtctt atgttgcaac
    26161 aattatttta ggtggaaagt atgtcttgga aggcacgatc tcgatcgggc agatggtctc
    26221 atttattagt tatatcggta tgcttgtttg gccgatgttt gcgattggtc gattgttcaa
    26281 cgtattggaa agaggaaatg cttcttatga tcgcgtcaat gaacttttgc atgaaaagac
    26341 gcatatcgtg gaacaactgg gtgcagtgca gaccccggca aaaggcgagc tgaatgtagc
    26401 tatttcctct tttaattatc cgaaagaaga atcaccagca ttagaacaaa tcaagtttgc
    26461 gatccaagaa ggagaaacat taggtattgt tgggaaaact ggtgcgggaa aaacgacgat
    26521 catcaaatta ctgatgcgtg aatacgatga ttatcaaggg aaaatccaat ttggcggacg
    26581 ttcgatcaaa gaatattcat tgaatgcttt attaggatca atcggctatg tgcctcagga
    26641 tcacttcttg ttttcgatga ccattcgaga taatatccgt ttttctaatc caaaactttc
    26701 agaagaagaa gtagagcatg cggcttcttt agcatttatc aacaatgaaa tcaaggaatt
    26761 accaaaaggt tatgatacct tagttggaga acgtggagtc tctttatctg gcggacagaa
    26821 acaaaggatt tcgattgctc gagcattgat caaaaatcct gaattattaa tattagatga
    26881 tgcactctct gcagtagatg ccaaaacaga agaagctatc ttgcataatt taaaagaaat
    26941 aagaaaagag aaaacaacta tcattgcagc tcaccgttta agtagcgtga tgcatgcaaa
    27001 agaaatcatt gttgtcgatc aagggaaaat cattgaacga ggaacccatc atgagctgct
    27061 tgcactaggc ggctggtatg aacaaatgtg ggagaaacag caattagaag cgaagataga
    27121 aggaggcgag gcctgatgga tgaaaattat caatcggaat ggacaaaatc gattccactt
    27181 aaagaacaga aaaagatcat caaacgactg atgaagttcg ccaaaccttt tagaagaacg
    27241 ttcatcgttg ctatcttatt tgcgtttgct ttgtctgtga tcaatgtcct attgccaagg
    27301 atcatccaaa cttttatgga tgatcatctg gctaaacagt cggctacgac acaagttatt
    27361 ttgttttttg ctggtatgta cttatttgga gtcatagtaa aaagtatcat ttggtttttc
    27421 caatggtatt tgtattccat ggcctcattg aagacctatc agtatattag ggtaaaatta
    27481 tttgaaaagc tgcatacact aggtatgcgt tattttgatc agacaccggc aggatcaacc
    27541 gtttctcgag tgacgaatga cacagaaacg ctatttgaat tttggtacgt ctttttgatg
    27601 gtattgactg ggatctttgc agtcgtttct tcattccttg ccatgtttca aatcaatgga
    27661 gaaattgcct tgtggacttt ggtgtttttg ccgattttag gaatcgtcat ctggtattac
    27721 cagaaattca gttctcgtat ttaccgaaat atgcgggaaa aattaagtca gctgaacacg
    27781 aaattgaatg aatcgatttc cggcatgcgt attatccagc agtttcgcca agaagctcgt
    27841 ttagctaaag aatttgaaca agtgaacaat gagtatttag acacgcgtta tgcgatgatc
    27901 aaaacgaatt cgattctgtt aagtccgatc atcaattttc tatatgcatt ggcgattgcg
    27961 ttggtgttga ctttgtttgg gatcgatgcg ttgcattctc ccgtagaagt tggactgatc
    28021 tatgcattta cgacctatgt ccagggattt tttaatccaa tgtctcaaat gatggacttt
    28081 ttgagcatct tcacagatgg gatggttgct ggtagtcgta tcttgaagat tatggatacg
    28141 gaagaatatg caccacaaca aaaccctgga gcagatcaaa tgattacttc aggtaagatt
    28201 gagtttcgca atgtaagctt ttcttatgat ggagagcaca atgtattaaa caatatctct
    28261 tttgttgcta atccgggaga gaccgttgca ttagtgggac acaccggaag cggaaaaagc
    28321 tcgatcatca acgtattgat gcggttttac gaattttatg aaggtcagat attgattgat
    28381 gatcatgaca ttcgagaata tccaatggaa gagttacgta agaaaatggg gctggtcttg
    28441 caagatgctt tcttgtttta tggggatatc gcaggaaata ttcggatgat gaatccagct
    28501 atcacagacg aacagatacg ccatgcggct gagtttgttc aagcaaacaa attcatcgat
    28561 gaattaccaa aaggatatca ctcaaaagtg atcgaacatg gtgccagcta ttctagtgga
    28621 caacgacagc tgatcacttt tgcgcgaaca atcgtgactg atcctaaaat tctagtattg
    28681 gatgaagcaa cagcaaatat cgatactgaa acagaaggat tgatccaaga aggattggca
    28741 aaaatgcgtc aaggacggac aacgattgcg attgcccatc ggctgtcaac gattcgagat
    28801 gcagaattga ttttagtttt agacaaaggt catatagtag aacgcgggaa tcatgaaaca
    28861 cttctagcgc aaaatggttt gtatgcagat atgtacaaac tgcaaagcag tggtatataa
    28921 aagatgttgt gacagtagaa ttttgaccta agcactagag tgaaaataaa acatagcttc
    28981 tcatattttt catctgtgag agttagtgtc gtagtcacca gatttaaaaa aagattcgac
    29041 agctagtcag aagtgcctaa tagccactaa gaaagcgttg acaaaagcat ttgctttcga
    29101 taatataaag aaaacgatac aacttggata gtgattgcct gatgcaagct aaacctgatg
    29161 agtctcccat ggtttcatgg acggcttttt gggtttttct ttttgttttg gatacagccc
    29221 tatacatcat ggaaaggagc aaaacatgct gattgagaaa gtactgaata acaacgtcgt
    29281 aataaccagg gatgaaagaa atcaggaaat gatcgtgatg ggaaaaggtt tggcattcaa
    29341 aaagaaagtg ggtgacccga tttttgactc atgcatcgac aaaaaatttc agttgacaaa
    29401 tgacgggctt tcacaacagt tccaagaatt ggcacaagcg attcctcttg agtacttaga
    29461 aatttcttgt gaaatcattg aatatacaaa agaggctcta caagtccagt tgaatgattc
    29521 tgtgtatatt gcattgacgg atcatttgta tacggctatc gaacgtcaca aaatgggaat
    29581 atttgtacca aatgtattgc tgtatgatat caaacaacta tttcctaaag agtatgccat
    29641 cgggaaaaaa acagtgaaga aaatctcgga taaataccac atcaagttat cagaagatga
    29701 agccggattt atcgcattgc atttagtcaa tgcacaaggc attcaaagcc agcaagacaa
    29761 tacctacctg tttactataa caatacagga tattctaaat atcgtccgct attactataa
    29821 gattacattt gatgaagatt cagtccattt ttttcgattc gtcacacact tacgtttttt
    29881 tctgacacgc gtcctagatg gagaaaaatc agaagtggga gaagtttctg aggaattgtt
    29941 tcaggttatt caaaaaaaat ataaggatac gtttggttgc gttgagaaaa tagcagactt
    30001 tttagccgag acgttcaact accagatgtc cacggacgaa aaaatgtatt taacgatcca
    30061 tactgcgcgt ttagtgcatg agaatcgttg taaataaaaa aaggaggata aattaacaag
    30121 aacgtatgaa ataagggtag tgacattcag ttggctaaac cttccactaa taaattggag
    30181 ggaatagaaa tgggaaaata ccaagaatta gcgagaaaaa tcgtagaaaa tgtcggcggg
    30241 aaggaaaata tcaatggttt gacacattgt atcacaaggc ttcgtttcaa attaaaaaac
    30301 gaagaaaaag caaatgatga gatcttgaaa aatatggacg gtattgtaac ggtaatgcga
    30361 gcgggagggc agtatcaagt tgtcattggg aatcatgtgc cagtcgtgtt tgaagaggtt
    30421 ataaaagcag gaaaccttac ttttgatgag gctgtttcaa cagaaaaaat gagaccgttt
    30481 gatatgctta ttgatggaat atcgggctgt ttccagccat tccttgctat cctagctgct
    30541 ggcgggatga ttcgtggact gacagcattc cttgtcttct taggcgcgtt tgaccgcggt
    30601 tctggcacat ttgttatgtt tgataatatc ggtgattctg tattccaatt tatgcctgtt
    30661 attatcggtt taacagctgc ccgtaaattt aaagtaaatg aattcgtagg gatgttgctt
    30721 ggcgcagcat taatgaatcc tagcttgtcg ctggaagctt tgagtggtgc agcagaagca
    30781 ccactaacga ccatattttc tggcactatt tttgaagcac ctatttatca aacagtattc
    30841 ggcattccct ggattgcgcg taactacgct tcttctgtca ttcccattat ctttattgtg
    30901 ttgttagcta gccaaattca gaaaccaatt aaaaagttag tacctgagat gatcgcgaat
    30961 ttcttcgttc cattcttcac cgttcttatt acaatgcctt taggtttttt actcgttgga
    31021 ccagtattca cattcgcaac tgatatttta atggctggat ttgaaacact tttagcactt
    31081 tcgccagtta tttatggagc aatcgtaggc ttcttctggc aaattctggt gatgtttggt
    31141 ctccattggg caatcgtacc aatgggattg atgcaatttt ctgtgaatgg ttggcaaaat
    31201 attatgacac ctgttgcagt agtgtctttt ggacaaactg cagcacttac cgcactctac
    31261 tttaagctta gaaatccaaa agacaaagca attgcaatac cagccattgt ttctggtatt
    31321 gtggggatta cagagccagc tatttatggg tttacattac cacgtaaaaa aatatttatt
    31381 tttacctgtg tcggtggtgc gttagggggc gcgtatagtg ggttaatgaa tttaacttct
    31441 tggaatcaag gtggactagg catctttaca attcccaact atattagacc agatggtgat
    31501 ttaacagatg tgattaatgt attgattggg atcgccattg cgatggtggt atcctttacg
    31561 ctgacttttt tcttttggaa ggatgaagca ggtgaaacag atgatataca gaaaaaatca
    31621 ggtaaagaaa ttgtaaaaac accgatacaa ggacaaatcg ctccactaaa tgcagcaaag
    31681 gatgcagctt ttgcacaagg aacactaggt cgaggaatct tgatctaccc tgaaaaagga
    31741 gaagtacgtg ccccttttga cggaacagtc atgaccctgt tcccgacgaa acaggcaatt
    31801 ggaatggttt ctgaaacagg gctggaattg ctgattcatg tcggattgga tactgtgcag
    31861 ttggaaggga aatattttga aagcttagtg cagcaaggat aaagagtgaa acaaggcgat
    31921 ctgttgctac gcttcgacaa aaagaaaatc gaaaaggaag gatacagttt agaaacacct
    31981 gtgatcgtca ccaattacac ggattatttg gatattttgg aaaaccaagg ggagacggtc
    32041 gggccagacg atgcacttat caccgcactg acctgaacgg ttaggtgaag aatggactag
    32101 gcttaactat tctttttatt ccacttcttt attttcaaaa ttgaaaggct ctttcatgat
    32161 ggctgagctg atagggcgtt tatcgaattt ttgaccgaca gaaattagat ggactaaatt
    32221 ctgttgataa ttttctgcaa aaacacaact atataacacg tctagaagat aacaaattga
    32281 tgtattgatc gtaaaattac caatttttga atacagtcgc tcgcgtgtcg taagtcgcaa
    32341 agcggctgta ctcattgcag atagcgttga atcaccgata ctggtcaaag ctaaaattgg
    32401 aacatgattt tgtttcaata tttctgcggt acgaaggatc acattgtttt cacccgtata
    32461 agaaatcaga atcccgcaca tatcactcgt gcaattgtac gcttcataga atgattcacc
    32521 aaaagtggtg acaatcgtga catccatttt gatgcgcttc attttcagca tgaaatcttg
    32581 agaaatcaaa gtattcgagt tactggcgaa aatcacgatt ttttttgctt gaagcattag
    32641 ttgtttggct ttttgcagtt ggtcatgatg aatgagagaa agtgtatcat gaatcgtcat
    32701 ctgttctaaa gaagccattt tatttgcgat cgtcataata ccgtctgtgg tggaaaaagg
    32761 aagattcgca tctacgtctt gaaaattccc ttcaagatag gctgtttcag ctaaaaaagc
    32821 attacgaaaa ctgacccagc catcaaaacc catttttttt gctacacgga tgagtgtaga
    32881 ggggtggaca taacatcgct cagcaatcgc ttggatggtc atttcgccaa gtgctggaga
    32941 atcgcttaag agaaactgga tcacttcatt ttctgaagga gaaaaggaga tttctttgat
    33001 tttttcacgt aataacatgt agataccttc ttcttatttt tttacgtaaa ttataacttt
    33061 tttgaaaatg attt
//
LOCUS       DAGCYL010000012        31640 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.012, whole genome shotgun sequence.
ACCESSION   DAGCYL010000012 DAGCYL010000000
VERSION     DAGCYL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.012"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(135..512)
                     /locus_tag="MB216_000418"
     CDS             complement(135..512)
                     /locus_tag="MB216_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525279.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            768..1496
                     /locus_tag="MB216_000419"
     CDS             768..1496
                     /locus_tag="MB216_000419"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBT4525280.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1496..1750
                     /gene="purS"
                     /locus_tag="MB216_000420"
     CDS             1496..1750
                     /gene="purS"
                     /locus_tag="MB216_000420"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBT4525281.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1752..2423
                     /gene="purQ"
                     /locus_tag="MB216_000421"
     CDS             1752..2423
                     /gene="purQ"
                     /locus_tag="MB216_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBT4525282.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2424..4646
                     /gene="purL"
                     /locus_tag="MB216_000422"
     CDS             2424..4646
                     /gene="purL"
                     /locus_tag="MB216_000422"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBT4525283.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4631..6070
                     /locus_tag="MB216_000423"
     CDS             4631..6070
                     /locus_tag="MB216_000423"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBT4525284.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6093..7145
                     /locus_tag="MB216_000424"
     CDS             6093..7145
                     /locus_tag="MB216_000424"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBT4525285.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7142..7720
                     /gene="purN"
                     /locus_tag="MB216_000425"
     CDS             7142..7720
                     /gene="purN"
                     /locus_tag="MB216_000425"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBT4525286.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7717..9252
                     /gene="purH"
                     /locus_tag="MB216_000426"
     CDS             7717..9252
                     /gene="purH"
                     /locus_tag="MB216_000426"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBT4525287.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9268..10542
                     /gene="purD"
                     /locus_tag="MB216_000427"
     CDS             9268..10542
                     /gene="purD"
                     /locus_tag="MB216_000427"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBT4525288.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10664..11578
                     /locus_tag="MB216_000428"
     CDS             10664..11578
                     /locus_tag="MB216_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBT4525289.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11711..13276)
                     /locus_tag="MB216_000429"
     CDS             complement(11711..13276)
                     /locus_tag="MB216_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBT4525290.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13269..14720)
                     /locus_tag="MB216_000430"
     CDS             complement(13269..14720)
                     /locus_tag="MB216_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBT4525291.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14734..15750)
                     /locus_tag="MB216_000431"
     CDS             complement(14734..15750)
                     /locus_tag="MB216_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4525292.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15970..17058
                     /locus_tag="MB216_000432"
     CDS             15970..17058
                     /locus_tag="MB216_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBT4525293.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17112..18638
                     /locus_tag="MB216_000433"
     CDS             17112..18638
                     /locus_tag="MB216_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBT4525294.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18640..19845
                     /locus_tag="MB216_000434"
     CDS             18640..19845
                     /locus_tag="MB216_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4525295.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20102..21079
                     /gene="guaC"
                     /locus_tag="MB216_000435"
     CDS             20102..21079
                     /gene="guaC"
                     /locus_tag="MB216_000435"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBT4525296.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21343..22767
                     /locus_tag="MB216_000436"
     CDS             21343..22767
                     /locus_tag="MB216_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBT4525297.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23228..24337
                     /gene="pdhA"
                     /locus_tag="MB216_000437"
     CDS             23228..24337
                     /gene="pdhA"
                     /locus_tag="MB216_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBT4525298.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24340..25317
                     /locus_tag="MB216_000438"
     CDS             24340..25317
                     /locus_tag="MB216_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBT4525299.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25342..26985
                     /locus_tag="MB216_000439"
     CDS             25342..26985
                     /locus_tag="MB216_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBT4525300.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26993..28399
                     /gene="lpdA"
                     /locus_tag="MB216_000440"
     CDS             26993..28399
                     /gene="lpdA"
                     /locus_tag="MB216_000440"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBT4525301.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28561..28836
                     /locus_tag="MB216_000441"
     CDS             28561..28836
                     /locus_tag="MB216_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBT4525302.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28842..29609
                     /locus_tag="MB216_000442"
     CDS             28842..29609
                     /locus_tag="MB216_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBT4525303.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29777..31606
                     /gene="typA"
                     /locus_tag="MB216_000443"
     CDS             29777..31606
                     /gene="typA"
                     /locus_tag="MB216_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBT4525304.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
BASE COUNT        10355 a         5434 c         7117 g         8734 t
ORIGIN      
        1 aaccaaaaaa gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt
       61 atcaataata ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac
      121 aaaaaataga ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg
      181 agatgttgca gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc
      241 tggatacgat aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg
      301 cctttgtgtt ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt
      361 gctcggcgga tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct
      421 ttgatcgaca gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa
      481 aaaagctgac cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc
      541 caaattttta gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg
      601 gtataagatt ttcgtttctg ataaaacacg aacattaaaa gaatctaatg ccttaaatgt
      661 acatattttt gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca
      721 aaaaacgatg tatttgcttt taatgtaaat aaaaaggaga gacgtacatg gagaagaaag
      781 aacttttgta tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag aacttgattt
      841 ggatagaata tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac cagataagtg
      901 gaaaaggaga attgaataat caaatcacaa gtgcaatttt ccgttatctg actcaaagag
      961 gaataaaaaa tcattttatt gaacaattgt ctaaaaccga acaattagca aaaaaagtac
     1021 aaattatccc tctggaagtg gttatccgaa atattgcagc aggaagtttc tcaaaacgtc
     1081 taggtgttcc cgaaggtaaa gaattcatca aaccaatcat tgaattctac tataaggatg
     1141 accagttgga tgatccattt atcaatgaag agcatatcta tttcttacaa cttgcaaagc
     1201 cggaagaaat cagtgagctg aaagcccaag cgttggctat taatgaaatt ttgaccgaat
     1261 tatttgctaa ggtcggtttg gttttagtag atttcaagct agaatttggc cggtcgaatg
     1321 gacaaatttt gcttgcggat gaagtaacac cggatacttg ccgattatgg gatgaacgta
     1381 cgaatgaaca tatggacaaa gacgtttacc gcagagatct aggtgatttg atcccagtct
     1441 atcaagaagt atataaccgt ctatcggaac taactgaaga aaaggagcga gtatgatgta
     1501 tttcgtaaaa gtatatgtaa cctataaaga ttcagtgtta gatcctcaag gggaggcagt
     1561 aaaaaaagca gtccatcgta tgggatatga aacaatcgaa gatatccgaa taggaaaata
     1621 ctttgagata caacttgctg atgcagaaga accaatcgat acagtgattg aaacgatttg
     1681 tgacaagctg cttgcaaatg taaatatgga aacataccga tatgaaatca aaaaaatgga
     1741 ggaagtataa catgaaattt gcggtcatcg tttttccagg ttcaaattgt gacgcagata
     1801 tgttatgggc agttcgcgag atcatgggcg ctgatgccga atttgtacgg catgatgagt
     1861 cctctttaga aggatttgat ggggttttac tgccgggagg attctcctat ggtgactatt
     1921 tgagatgcgg tgcgattgct cggttttcta caatcatgga agaagtgatt cgttttgctg
     1981 aagaagggaa accagtattc ggtacatgca atggattcca gatattgaca gaagcaggac
     2041 ttttgccagg tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa actgtaccat
     2101 tgaaagtcaa ccccaagacg gcctttactt cagagtatga agccgacgaa ataatctatc
     2161 ttccagttgc tcatggggaa ggaaattatt tctgtgacga agaaacactt caagaactaa
     2221 aaaaaaataa tcagattgcc tttacttata atgacaacat caacggcagc gtggaaaaca
     2281 ttgcagggat tacgaacaaa gcagggaatg tgttaggaat gatgcctcat ccagaacgtg
     2341 cagtagaaga cttgctagga tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa
     2401 acttcggaaa ggtgaccgta tagatgaaaa cattgaaaga accgacagct gaagagatca
     2461 agaaaaaaag aatctatgcc gaatggggat tgactgatga agaatatcga atgatcgaag
     2521 aagatatctt aggaagaatg cctaattata cggaaactgg actgttttct gttatgtgga
     2581 gcgaacattg ttcctataaa aattctaaac cggttctacg caaatttcct actagtggac
     2641 ctcatgtgtt gcaaggccct ggagaaggcg caggcatcgt cgacatcggt gatggactag
     2701 cagttgtctt caaagcagaa agtcacaatc atccttcagc cgtggagcct tatgaaggag
     2761 ctgcgacagg agttggcgga atcatcagag atattttcag catgggggct cgtccaattg
     2821 cgttgctaga ttcattgagg ttcggcgaat tagataatcc aagaacgaaa tatttactag
     2881 aagaagtagt tgcaggtatc ggcggatacg gcaattgtat cggcataccg actgtaggcg
     2941 gcgaaattgc atttgatcct tgttatgaag gaaacccatt agtcaatgca atgtgcgtag
     3001 gtttgattga acacaaacat atccagaaag ggcaagccgc tggcgtcggt aattcgatca
     3061 tgtatgtagg tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt gcctcagaag
     3121 aatttaatca agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg
     3181 aaaagctgct gcttgaagcg tgtttggaat taatcttgca tcattcggat tctctaatag
     3241 gtatacagga tatgggagca gcaggacttg tttcatctag tgcggaaatg gcttcaaaag
     3301 ctggctcagg acttatattg acattagatg aagtcccaca aagagaaact gggatgacac
     3361 cttatgaaat gatgttatcg gaatcacaag aacggatgtt gatttgtgtt aaatcaggag
     3421 aggaagaaaa aatccaagaa ctatttcaga agtatgatct agatgcagtc actatcggta
     3481 aagtaacaga cgacgggcaa tatcggctat atcatcaagg agaagaagta gcaaatctgc
     3541 cagtcgatgc attagctgaa gatgcaccag tttatcataa agaaatgaaa gaaccagctc
     3601 gaatcgtaga atttcagcag ttagctcctt atcagccagt tatcgaagaa cctggacaag
     3661 tattgcttga tctgctgcag caaccgacga ttgcttcaaa acgttcaatc tacgaaactt
     3721 atgattcaca agtacaaacc aatacagttg tccgtccagg aagcgatgct gctgtgatga
     3781 gggttagagg gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt
     3841 atttggatcc aaaaatcgga ggacaaatag cagtggcaga agctgctaga aatattattg
     3901 ctagcggtgg taagcccttg gcgatcaccg actgcctgaa ctatggttct cctgataaac
     3961 cagaagtttt ttgggaatta tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt
     4021 taggaactcc tgttatttca ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa
     4081 tctatccaac accaatgatt ggtatggtcg gattgattga ggatcataaa catatcacta
     4141 ctcaggaatt caaaaaatca ggggatctaa tctatatatt aggtaagacg tttgcagatt
     4201 ttgatggcag tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg atccgaaact
     4261 ttgatctatc cattgaaaag agaaaccaag agttagtatt gacagccatc caaaacggat
     4321 tgattgaaag tgctcatgac tgttcagaag gtggcttggc aattgcactg gcagaatcag
     4381 ctttcaaaca tcagctaggg atatctgtac agtttgaatt gtcctctgct caattatttg
     4441 cagaaactca atcaagattt gtgttgacag tcgcaccgga aaataagaca cgattcgaag
     4501 aaatgatggg agatgctgct gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa
     4561 tttcagcaac tgacggtcag atcaaaatag aaactgcagt agcaagaaag tgctgggagg
     4621 atgcaatcgt atgtcttatg aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt
     4681 ttggggtcat ccagacgccg ctagtgtggc atacttcggg ctccatagtc ttcagcatcg
     4741 aggacaagaa ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga
     4801 tttggggctg ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga
     4861 agctgcaatc ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg acaatattca
     4921 gccttttcta ttcaaatttc atgatcagtc gatcggctta gcgcataatg gtaacttgat
     4981 caatgctcgt tcccttagaa aagagttaga ggaaaatggg gcgatcttcc attctaactc
     5041 cgatacggag atattgatgc atttgatccg acgaagtgaa gaaacagatt ttttaaataa
     5101 attaaaaagt gctttgaatg aagtcaaagg tggctttgcc tatttattaa tgacagaaac
     5161 ggcaatgatt gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa
     5221 aaacggagca tacgtgattg cctcggaaac ttgtgcatta gaagttattg gggcacgttt
     5281 tataagagat gtccagcctg gtgagatagt gatcatcaat gacgaaggaa tccagattga
     5341 ttgctttacg aaggacacgc agctttctat ttgttcgatg gaatatatct attttgccag
     5401 accagattca aacatcgcag ggatcaatgt gcatactgca cggaaaaata tgggacggaa
     5461 tttagcgaaa gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta attcttcttt
     5521 gtcagccgca agcggttatg cggaagctag tggaatccct tatgaaatcg gattagtaaa
     5581 aaatcaatac attgcccgaa catttatcca gccaacgcct gagctaagag agcaaggtgt
     5641 tcgaatgaaa ttatctgctg tacgaggagt agtggaaggt aaaaaggtga ttatggtcga
     5701 tgattcaatt gttcggggta cgacaagcag acggatcgtc catcttttga aagaagcagg
     5761 ggcaaaagaa gtccatgtga ggattgcttc tcctcctttg aaatatccat gtttttacgg
     5821 gattgatatt caaacgcgta aagaactcat tgcggcaaat cacacgattg atgaaatcaa
     5881 agaatgtatc ggtgcagatt ctttaggttt tttaagtgaa gagggtctga tcgaatcgat
     5941 cggtctggaa aaagatgctc cgtattcagg attgtgtatg gcttatttca acggagatta
     6001 tccgacgccg ttatatgact atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc
     6061 gttcttttaa aagcagacca agaagggagt atatgaattc aatggcaaat gcatacgcaa
     6121 aagctggcgt agatgtcgaa gctggatatg aagtagtaga acggatcaag aagcatgtca
     6181 aaaaaacgga acgtttaggt atgatgggcg ctataggagg attcggcggt tgttttgatt
     6241 tatcggtctt tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc gtggggacga
     6301 aattagcagt ggcaatcgaa gcagacaaac acgatacaat cgggatcgac tgcgtagcga
     6361 tgtgtgtcaa tgatatctta gcacaaggcg cagagccgtt atatttctta gactacttag
     6421 caacggggaa aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat
     6481 gtgttcaagg aaatatggcg ctgattggcg gagaaacagc tgaaatgcct ggtatgtact
     6541 caggtaatga ttatgatcta gccggatttg ctgtagggat agctgaaaaa tctcaattat
     6601 tgacaggaga aacgatcaac ccaggagatg tgttgcttgg aatagcatct agcgggattc
     6661 attcaaatgg gtactctctt gttcgcaaaa ttttctttga gcagcagcag ttttctttat
     6721 catcaaaact gccagaattg aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa
     6781 tctatgtttc ttccttgctt ccgctgatca aagaaaagaa aataaaagga gctgcacata
     6841 tcactggtgg agggtttgta gaaaatatcc cgcgaatgtt accagaatct atggctgcac
     6901 aaatcaaact tggctcatgg ccgattcttc caatattcgg ctgtttacaa aaatatggtg
     6961 aaattcctga gatggagatg tatgaaatct tcaatatggg catcggtatg gtcctggcag
     7021 tcgaagaatc gaaagtaaaa gaaatagtac acgaaattga gcaagctggc gaaaaagttt
     7081 atgaaatcgg gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac
     7141 gatgagaatc gctgtttttg cttctggaaa cggaagcaac tttcaagcgc tagccgatta
     7201 tctatcgaaa aaaggattgg aatcttcgat tgactggttg ttttgtgatc agcctgaagc
     7261 atatgttttg aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt cacctaaaga
     7321 atttgattca aaaaaagagt atgaagaagc aattttgcat aaattgaaag aaaaaaagat
     7381 cgatttgatc gtattagctg gatatatgcg aatcattgga ccggtattgc tgaaaaatta
     7441 tgataaaaga atcatcaata tccatccgtc tcttttacct gccttccctg gtttgcatgg
     7501 catacgtgat gcctttgaag ctggagtaaa agaaacgggt gtcacaattc attatatcga
     7561 ccaaggtgta gatactggac caatcatccg gcaggaaaaa gtgagaattg aacaggagga
     7621 cacgttcgat tctttagaag aaaaaatcca tagagtagag catcgaatct atccagaagt
     7681 gattagtgaa attattgaaa atggaggcta tctgaaatga caagagcatt gatcagtgta
     7741 tcagataaaa ctgggattgt agcattcgcc caaggtttga ttgaacaagg agtagaaatc
     7801 atttcaacag gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac gctttcgatc
     7861 gaagaagtga cagggtttcc tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa
     7921 atccatggtg gtcttttagg aagacgcgat ctccctagcc atatggaggc aatggaaacg
     7981 gaaaatatcc aaccaatcga ctttgtttgt gtcaatttat atcctttcaa agaaacgatt
     8041 atgaaaccag atgtcgaaat agcagaagct attgaaaaca ttgatatcgg tggtcctagt
     8101 atgctccgta gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga tccatctgac
     8161 tatgaagaag tactttcaga attacgtcag tatgggcata cttcactttc tttcagacaa
     8221 aaactagcgg caaaagtctt tcgtcatact gcggcatatg atgcactgat cgctcaatac
     8281 ttaacggatt gggtggaaga aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa
     8341 caaacgcttc gttacggtga aaatagccat caagccgctg ccttttatca gtccgctctt
     8401 ctggaaaaat attcgatcgc agcagcaaaa caactccatg ggaaagaact ttcttataat
     8461 aatatccgag atgcagatgc tgcgctacgg atcatgaagg aatatgacga gccaacggta
     8521 gtcgccttga aacacatgaa tccttgtggt attgggacag catctacgat tttagaagcg
     8581 tgggaagcag cgtatgaagc agatcctgtt tcaatctttg gtgggatcat cgttttgaat
     8641 cgagaagtag atctgccaac agcagaacag atgcataagc tgtttctaga aatcatcatt
     8701 gccccttctt atacacaaga agctttggaa ttactgcaaa aaaagaaaaa catccgatta
     8761 ctgacagtag actttgcgca ggaagaaaat caaaatcgag aagaaacggt ctcagtctta
     8821 ggtggacttc ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc
     8881 gtgacaaaac gtaagccgac tccagcagaa gcaaaagcat tagcgtttgc ttggagagct
     8941 gtcaaacatg tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc
     9001 ggagccgggc agatgaatcg agtcggttca gtaaaaattg cgattgaaca agcgcaagag
     9061 aaaatggaag gtgcagtttt agctagcgat gcttattttc caatggatga ttcggttgaa
     9121 tatgcagcca aacatggtat aaaagcaatc attcagccag gaggaagcat caaagaccaa
     9181 gattctattg atatggcgaa taaatacgat ctagcaatgg tctttactga tgttcggcat
     9241 tttagacatt agagaggagt cggataaatg aacattttgg tgatcggtgc aggtggtcgt
     9301 gaacatgcaa taagtaaaaa acttttagcc agtccgaagg tggatacggt gtattgtgcg
     9361 ccaggaaatc cggggatgac aaaagatggt atcaattgtg tcgctatcag tgagcgagat
     9421 cattcagcgt taatccattt tgttaagaaa gaggctattg attggagtat cgttggtcct
     9481 gaagttcctt tgttaaatgg gatcgtcgat gattttatgg cggaaggatt gaaaatcttc
     9541 ggtccgacaa aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg
     9601 gatacttatc aaatcccgac agctaaatcc agaagttttg aatgttacga aaaagcagca
     9661 gcgtatatca aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt agcagctggg
     9721 aaaggtgtag tcgtggctac cacagtgaag gaagcgctgg atgcggcaga acaaatgttg
     9781 aaccagcatc gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga
     9841 gaagaattct cccttttggc gtttgtccgt aataccaagg tgtatccgat ggtcatttcg
     9901 caagatcata agcgggcatt tgataacgat aaagggccaa atacaggcgg aatgggtgct
     9961 tattcaccag taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga agaagtttta
    10021 aaaccagcgg ctttgggaat gaaagaacta ggacgctctt tcacgggtgt tttatatgcg
    10081 ggtttgattg ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat
    10141 cctgaaacgc aagtcgttct ttctcggttg aagagtgact ttgcgcaagt aatcgatgat
    10201 ttattagaga atcgtacagt agagttaaag tggcagcaag aaggatacaa tgtaggcgtt
    10261 gtggttgcag ctgccggtta ccccgaagaa tatgagacag gaatggttct accagatttt
    10321 ctggaagaag agctttctgt ttactatgct ggagtttcct caaataacgg caaactagtt
    10381 ggagcaggag gacgtcttta tttagtagaa gcatacggtg agacgatcaa agaagcacag
    10441 aaaaaaattt acaccgcgtt aatggataaa acgacagatg gaacatttta tcgtacagat
    10501 attggagaaa aagctgccaa tagcgaactg atcgtgaagt aaatcagctg cttccattta
    10561 taaaaaatac aacaaacata atcggaagcg ccgattttag tgaagccttt tcataaagag
    10621 tgtggtacac ttttgttgtg tatataaata aggaggagtt cagatgaaaa aaattcaatt
    10681 aggtacttct gatcagaaag cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc
    10741 ggaagatcca gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt tttttgacca
    10801 tgcagatatt tatggaaacg gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac
    10861 atcaatcaaa cgagaagatc tttttattca aacaaaatgt ggaatcgttc caggtgtgat
    10921 gtttgatttt tctaaaaaac atatcattga atctgtagaa aaaagtttga cccgtctgaa
    10981 aatggattat gtggatgctt tacttcttca tcgtccagat acattggtag aaccagagga
    11041 agtagctgaa gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag
    11101 caatcaaaaa ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac cattagtagc
    11161 taatcagctg caatttggct tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa
    11221 tatgacagat gaagcaagta tcgatcatga cggtagtgta cttgattatt cccgattgca
    11281 tgatatgacg atccaagcat ggtcaccata ccagtatgga ttttttgaag gtgtatttat
    11341 cgggaacgaa aaattccctg aattaaacca aacattagaa gaactagcag ctaaatatca
    11401 aacgacacca actggcttgg cttctgcttg gattttacgc catccagcca atatgcaagt
    11461 gatcgccggt acgatgaacg tagggcgaat caaggaaatc gctcaagctt cagaaattaa
    11521 tatgtctcgg gaagattggt acgaattgta ccgtgctgct ggaaacattt taccttaaag
    11581 attgtcataa gcaggtcaaa agggctataa attaaaaaga cagaataact tgaaagctgt
    11641 tttcagcttc gagatattct gtcttttttt attgcaaatg gaaagagtat cattgtacgg
    11701 atttcgttta ttagttattt ttgtttttaa ggaaaagctg tgtccgatat tcttttggag
    11761 aaatcccttg ttttttctta aataaataac tgaaatagtt agggtcatta taaccaattt
    11821 caaaagcaat atctgaaata cgatagtctg attgctgcaa taattttttt gctaattcga
    11881 ttctttgttc tgtcagataa tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac
    11941 tgaaatgaga gggacttaat gcaacttgtt gagcgaccca agttaaggac atatctggat
    12001 cggtaaaatt ttctttgata aaatccaatg catggttcaa aattggctga tatttgttcc
    12061 ttgaaggctg tgagggcaga gcagttaaga aagaaatgaa catataaact gtcgaacggt
    12121 aaagcgagtc gtcggcgctg attgctaagc gttgactggt ggatgtgcaa tgaaccattt
    12181 cttgcgggat tttcttttct ttcaggcaga catgctggag tagcttagtc aagacaaata
    12241 aacgatataa tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact
    12301 tagatacttc gtttttcgat aatgaagaca aatcaagtga gaggtcaaag taaaagacag
    12361 aatcaactgg aagtttcgct tcttgaatca aatcctcata gctgataatt tgttctttaa
    12421 gggaactggc ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt aattcgctga
    12481 ttctttctgc gacaggacca atggcgacag aaaaattatt tttttgtccc ttttctaatt
    12541 catggaccaa cgtttgggct acctgataac tggtctcgat cacattttct tcaagagaat
    12601 caactattaa aaatttgatg aatcgagagg aaacagcaga gaagatcact tgttgattct
    12661 ccctaaataa aaacttcaaa taatcactga gatgagcgta atcttcaaag tctgtactga
    12721 cttggtttgc tgctaataat acggtcattt ttttccctgc taaagagcga ttgaaagatt
    12781 cagcaagttt caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat
    12841 gatttttttt caattcgaaa acaagatcag aagaagtgtg cgtttctttt tgctgcaaat
    12901 ctaaagcagt tactgctttt tctattgctt gtttcaattc gctactttta atcggtttaa
    12961 gcagataatc atctgcctgt acctggattg ctcctttcat ataatcgaaa tcgtcgaatc
    13021 cactaataaa tatgagacga gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca
    13081 acccgtccat gaaaggcatt tggatatcag tcagaatgat gtcgggctgt aagtcgataa
    13141 ttgaagacag tgccatctca ccatctccag cttcgccgac ataagtgatt ggataataag
    13201 aaccaaggac agttatttgt tcccgcaagt tattgcgaat gaggtattcg tcttcgatga
    13261 taaaaacttt atacatgttt gtttcctcca attgttttgg gagatagagg gatcatcagt
    13321 gtgacagttg ttccttctcg gtatttgctg tcaatcgtga taggtttttg atcatttccg
    13381 taataaagaa ggagacgctt gtttacgttg taaagtccgt agccgatact gaaatctgaa
    13441 tcgatccctt tggctaactc tttacgtatt tctgccagtt tttctgcagg gatgccaatc
    13501 ccgttgtcta taactttgaa ttggatcatc tcatcaattt tgcatgtggt aatacgaata
    13561 aatcctttgt gacggacgta cttcgttcca tggtatacag cattttcaat caatggctgc
    13621 aagatcattt ttaaaataga aacaggaaaa agtgtttctg gtatatcgat cttatagtca
    13681 agcatatggc cgtatcttat ttttaagatg actagataat cattgatatg gctaatttct
    13741 ttttcaagag gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt
    13801 gcatgggtca tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga aataatggca
    13861 tccaaactgt tataaataaa atggggagta atctgagctt gcaatacacg aacttcactt
    13921 tgtgctaaat ggtattgttt caaagcgttc tcccgtaaag actggtttag atcttccgcc
    13981 attttattca tgctagcggt aagtgtttgt aattccagtg tttccggcag ggttaaacga
    14041 aagccaaggt tccctgaaga aagttgttct gacatctcga tcagctgatt gatcggttct
    14101 tggacctttc tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat ggtactaaga
    14161 actccaagtt ccactattgt taatagaatg agcgagcgga caagtgtcgt attttttttg
    14221 cttgctaagt cgatctccac acgaacaaaa tcttgtaaga tatctgacag cagttgagtg
    14281 acagaatcta cctgattgat gatctcttca ttggtatcca taaaatcttt tgccaagata
    14341 ttattgatca gctcgttttg ataatcttcc agtgtatcga tcgtacgtag cgatacatct
    14401 aacgtactaa tttctttaag tgtatttgta ttatttttga tctctcgaat atcattccta
    14461 agttcttcta ggatattttc ctcattgaac tcatctacgg ttacttggcc agaaatcaag
    14521 tcccacatac tttccaaaac tttttgatca accttcaaag caattgcatc tgctttttgg
    14581 atatttgcga tcgtctgttg ataaatcaaa agctgttgcg aatataacag tatgctcagt
    14641 agcaaaggga taacagcaaa tagaagcatg gctttgtttg cttgggcaaa aatctgtttt
    14701 atgcttaaat tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc
    14761 tatattctgc tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc
    14821 tgtttgaata tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg
    14881 tttacactcg actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg
    14941 tccatcaata gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc
    15001 agctaaagca ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa
    15061 atcatttgtt tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc
    15121 gacaattcgg tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag
    15181 ggttggttta cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga
    15241 gttctctgaa ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg
    15301 attgctgaca aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt
    15361 gacgaaaagc tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc
    15421 tgctcgcttt gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat
    15481 gatcatatcc actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt
    15541 catgtagcca tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga
    15601 ttcagtatgc tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag
    15661 tgggtcaggt gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac
    15721 aagggctaaa aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta
    15781 ctatcttatc agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa
    15841 gaaacttaaa aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat
    15901 aaaaaaatca atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg
    15961 ggataaaaaa tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca
    16021 agcattctat taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta
    16081 ggaatcgcta tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc
    16141 tcggaattag aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa
    16201 aatcaagtag cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct
    16261 tctatcgatg gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag
    16321 gtgatcgcct atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt
    16381 gataattttg gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa
    16441 gaaggaaaag gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca
    16501 ctaatcaatt acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg
    16561 gtcgtgccat ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct
    16621 acggcgcaag caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat
    16681 gctgttttgt cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg
    16741 gggtatggga gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct
    16801 ggcgttaaat cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc
    16861 ttagcaaaaa atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc
    16921 aatgatactg aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca
    16981 gtatctgtcg ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa
    17041 tccgatctga aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga
    17101 ggataagaat tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg
    17161 gaatccgagc gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt
    17221 gcggagaaaa cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat
    17281 atggctccta tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga
    17341 aagatagtga agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc
    17401 tttcaataaa agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt
    17461 gggaactgac agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc
    17521 cgaatcttct agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag
    17581 cattatcaaa atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag
    17641 aagaaagcgc gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca
    17701 tcattatttc acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac
    17761 gggatggaca gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc
    17821 gcggaatggt gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag
    17881 aagtttattt taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca
    17941 taaacgacca catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga
    18001 tgggagcggg cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga
    18061 tatccggacg tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca
    18121 ttgcaaacgg actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga
    18181 tggatattcg ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat
    18241 tagataaaga aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa
    18301 caaactccat ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt
    18361 tatccaagtg gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga
    18421 tagatgttgg ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca
    18481 aagctgtctg tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct
    18541 atacgatgaa cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag
    18601 tcttgatgaa attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt
    18661 acggataaaa aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc
    18721 tacagcatgg tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt
    18781 attttctggc gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta
    18841 ctagctgcag ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca
    18901 gtgatggcgt ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg
    18961 tggatgacag ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt
    19021 tgggtagctt atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt
    19081 cgtggactga cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc
    19141 caaaaaatgt ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg
    19201 acattgattt taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga
    19261 aatcgtcgga aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg
    19321 gtatttacag cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat
    19381 ccgattattt tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa
    19441 acagtagcag gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca
    19501 gggatcaaaa caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct
    19561 ttagctggat tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg
    19621 atggaactgg atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt
    19681 ggaacaatca tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg
    19741 tcgattttag gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta
    19801 gccgttgtgc tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat
    19861 tcagaaaaaa gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat
    19921 tcattgtttc gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt
    19981 ctctttttgc tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa
    20041 gccttgatta aattaataat gttcgtgttt tgctggatgt gaacatcagg aggataatgt
    20101 aatgaaagta tttgattacg aagatattca attaattcct aataaatgta tcgtaaatag
    20161 ccgttctgaa tgtgatacaa cggtcacatt aggtaaacat acattcaaaa tgccagtagt
    20221 gcctgccaac atgcagacga ttattgatga aacaatcgca gaattcttag cggaaaatgg
    20281 ttatttctat atcatgcatc ggtttgatga agcagctcga attccgttta tcaaaaaaat
    20341 gaaaaagcgt ggattgatta cctcgatcag tgttggcgtc aagaaagagg aatattcctt
    20401 tatagagaag ttggcagaag aatcgctaaa tcctgattat atcactatcg acatcgcgca
    20461 tggacatgct aactcagtta ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac
    20521 ttttgtgatt gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg
    20581 cgcagatgca acaaaagtcg ggattggtcc aggaaaagta tgtattacaa aaatcaagac
    20641 aggctttggg actggaggct ggcaactggc agctcttaga tggtgtgcga aagcagctcg
    20701 caagccgatc atcgctgacg gtgggatcag aacacatggc gatattgcaa aatctgtccg
    20761 ttttggtgcg acaatggtca tgattggttc attgtttgcc ggtcacgagg aatctcctgg
    20821 cgaaacaaaa gtcgaaaatg gaatcgtgta taaagaatac tttggttctg cttcagaatt
    20881 ccaaaaaggc gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac acaaaggatc
    20941 tttgaaagat acactagtgg aaatgcaaca agaccttcaa tcatctattt cttatgctgg
    21001 aggaagagat ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt
    21061 taatggagac acaatctaaa aaataaataa aatattagga tgttgtgaaa aagttgtcct
    21121 gcattaagta ataagaacag ccaaacaaaa atagttcttt atatgttttg tttggctgga
    21181 attattatcg cagatacaag cttttgaaca tcattattca gaataaggga ctgtgataag
    21241 atttttgtca cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg
    21301 gtaaaatcaa aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag
    21361 aaaaaacaag gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac
    21421 ggaaggatca atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt
    21481 gtgaatcgtt ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg
    21541 agcaaacgtg gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca
    21601 agaatcgatt ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa
    21661 aaaataagag aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag
    21721 atgccagatg agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta
    21781 gcggaagaaa aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg
    21841 tatttggaaa gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc
    21901 ggtcagcaag caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct
    21961 gtagagtcac cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta
    22021 tttgggatac cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat
    22081 aaaaataaat tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg
    22141 atgagtcaaa aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt
    22201 attgaagacg atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata
    22261 cgaacgattg atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa
    22321 acaacgaaaa tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg
    22381 caagaaatga tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt
    22441 aatgaagcaa ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga
    22501 ctgataagcg cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt
    22561 tactatgcct ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta
    22621 gtagatatgg gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc
    22681 agaatcaatt acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa
    22741 attctagaaa agtggttaac agaataattt cacaaagtaa atagattaaa gaatataaca
    22801 gcttggattt aaatttcaag ctgtttcttt tttttcggaa aaagaatacg atataaagaa
    22861 actaagaaaa aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg
    22921 caacaaaact gtataacacc aacgttttta aatagaaaca gatggttgta agtttgatta
    22981 gaaaatagaa aaaatgcaga tgaaatataa aacgctttcc ttattgtgat tttattcact
    23041 aatgctgtta atatgcggat tggcataatt tcctttcatc gtgaaaattt gtgcttttag
    23101 cttgtatttc tcataaatcc atgataattt attaatgaag acggttaaat agtgatacag
    23161 ttttattcac tgtttcaatc gcagaatgaa gagattttct tatagatgag agaggaatgt
    23221 gtgacaaatg gcaaacaaaa aagcaccgat cgacttccaa gcactgctag atgaagtcaa
    23281 tgcagatttc ccagtatatc aagcacttga tcaagatgga aacgtagtta acaaagattt
    23341 agtacctgat ttatccgatg atgaactcgt tgaattaatg actcatatgg tatggtctcg
    23401 tgtattagac caacgttcaa cagcgctgaa ccgccaagga cgcctaggct tttttgcacc
    23461 aactgctgga caagaggcaa gtcagctggc tagtgcttac gcatttgata aagaagatgt
    23521 tcttcttcca ggttatcggg atgtacctca actagtaaaa catggtttac ctttatctca
    23581 agctttttta tggtctcgag gacatgctgc aggtaattct tatccggaag aattgaaagc
    23641 tctaccacca caaattatca tcggtgcaca atatgtacaa gcagccggtg ttgcattagg
    23701 tttgaagaaa cgaaacaaga aaaatgtcgt cttcacttat actggtgatg gcggttcatc
    23761 acaaggtgat ttttacgaag caatcaattt cgctggcgct taccatgcaa atgccgtatt
    23821 ttatatccaa aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa
    23881 aacacttgca caaaaagctg tcgcagctgg gattcctggt attcaagtcg atggaatgga
    23941 tccgcttgca gtatatactg tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg
    24001 accagtccta atcgaaacac tgacttatcg ttatggacca catacactat ctggtgacga
    24061 tccaacccgc taccgtacaa aagacacaga tgacgattgg cagaaaaaag atccattgat
    24121 ccgcttccgt aaatatttga cggaaaaagg actttggtct gaagaaaaag aagaacaagt
    24181 catcgaagca actaaagaag aaatcaaagc agctattgct gaagccgaca aagtaccaaa
    24241 acaaaaggtt tcagatttct tgaaaaacat gttcgaagta tctccacaat caatcaaaga
    24301 acaaattgca atttatgaag cgaaggagtc gaaataatca tggcacaaaa aacaatgatc
    24361 caagcaatca cagatgcctt agcacttgaa ctagaaaatg acgagaatgt cgttgttttt
    24421 ggtgaagatg ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag
    24481 ttcggagaag accgagtatt cgatactcct ttagctgaat ctggaatcgc aggattgagt
    24541 ttcggtcttg cgttagaagg atttcgtcca gttcctgaaa tccaattctt tggttttatt
    24601 ttcgaagcaa tggacgaagt agtagctcaa atggctcgta caagatatcg aatgagcggc
    24661 actagaaatc tacccatcac aattcgttcg ccatttggtg gcggtgttca tacaccagaa
    24721 ttgcattcgg ataatttaga aggtttgatc gctcagtctc ctggtattcg agtagtgatt
    24781 ccttccaacc cttatgatgc aaaaggacta ttgatttctt ctattagaag taacgatccg
    24841 gttgttttcc tagaacacat gaaactatat cgctcattcc gagaagaagt gccggatgaa
    24901 gcttacgaag ttcctttgga taaagcagca gttactcgtg agggaacaga tgtatctatt
    24961 atcacatacg gagcaatggt gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa
    25021 aatatatctg ttgaaatcat tgatcttcgt acagttgcgc cattagatgt agaaacgatc
    25081 attcaatccg tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg
    25141 ggcgttgccg cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc attagaagcc
    25201 ccaatcggac gagtttctgc acctgataca gtcttcccat ttggacaagc agaaaatgtc
    25261 tggttgccaa atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga attttaagaa
    25321 aagaagggac gaaacaaagt catggcttat caatttaaat tgccggatat cggtgaagga
    25381 atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag gagatacgat caatgaagat
    25441 gatacattac tagaagttca aaatgacaaa tcagtagaag aaatcccgtc acctgttaca
    25501 ggaacggtta aaaatgtgat tgttccagaa ggtaccgtag caaatgttgg ggatgtatta
    25561 gtagaaatcg atgcaccagg acacgaagac aatgaagggg acagtggtgt agccgcagaa
    25621 tcccaaactc cagctaaacc ggctgctgaa cccactgttg atacagaatc agctggatcg
    25681 tcaagcgaag gagtcttcca atttaagtta ccggatatcg gtgaaggaat cgcagaaggt
    25741 gaaatcgtca aatggttcgt caaaccagga gatacgatca atgaagatga tacattacta
    25801 gaagttcaaa atgacaaatc agtagaagaa attccgtcac ctgtcacagg gacagttaaa
    25861 aacgtgattg ttccagaagg cactgtagca aatgttgggg atgtattagt agaaatcgat
    25921 gctcctggac acaatagtgc accgtcaact agtgcgccat cagctgaagc accaaaagaa
    25981 aaagtggaaa catcaggttc agcaagtgtc gttgaagctg ccgatccaaa taaacgtgta
    26041 ttggctatgc catctgttcg tcaatttgcc cgtgaaaaag atgtagatat ctcacaagta
    26101 actgcaactg gaaaaggtgg cagagtaaca aaagaggaca tcgaaaactt cttggcaggt
    26161 ggtccaagca gtgcaccagc gaaatcagaa gcgccagaag cggcagcacc gaaagaagca
    26221 gctcctgcag cagaaagcaa accagctgca ccagcaaaac cattcaagtc caacttagga
    26281 gacttggaag aacgtgtagc aatgacgcca acacgtaaag ctatcgctaa agcaatggtc
    26341 aacagtaagc atacagctcc acacgtgaca ttgcatgatg aagtagaagt gtctaaacta
    26401 tgggataatc ggaaacgatt taaagaagtt gctgctgcaa atggtacgaa acttactttc
    26461 ttgccatatg tcgtgaaagc tttaacagcg actgttaaga aatacccagt attgaatgca
    26521 tctatcgacg atgcaaacca agaaatcgtt tataaacatt actacaatat cggtattgca
    26581 actgatacgg atcacggact atatgtacca aatgtgaaag atgcagaccg caaaggaatg
    26641 tttgctattg cagacgaaat caatgagaaa gcaaaattag ctcatgacgg caaactatct
    26701 gcagaagata tgcgcaatgg aacaattacg atcagcaata tcggatctgt aggtggcggt
    26761 tggtttacac cagtcatcaa ctatccagaa gtagctattt taggtgtagg aacgattgca
    26821 caacaaccaa tcgtaaatgc tgaaggcgaa atcgttgtcg gtcgtgtgat gaaactgtca
    26881 ttaagtttcg atcaccgtat cgtagatgga gcaactgcgc aacaagcaat gaataatatc
    26941 aaacgtcttt tagctgatcc agagctatta atgatggaag gatgataaac aaatggtagt
    27001 tggagatttt gctgtagaat tagatacagt tgtaattggt gcgggacctg gtggctatgt
    27061 agctgccatc cgcgctgctg aaatgggaca aaaagtagca atcattgaaa gagagtatat
    27121 tggcggtgtt tgtttgaatg tcggctgtat tccttcaaaa gcactgatag ctgcaggaca
    27181 ccattatcaa gagtcactag attctgaaat gtttggagta actagtgaaa acgtcaaact
    27241 agattttgca aaaacacaag aatggaaaga aaacaaagtt gtccatacgt tgacttctgg
    27301 tgttggtttc ctattaaaaa aacataaggt tgaaacaatc gaaggagaag cgttcttcgt
    27361 agatgatcac acactacgcg tcattcatcc agattcagct caaacttatt cgttcaataa
    27421 cgcaatcatc gcaacaggtt cccgtccaat cgagattcca ggatttaaat tcggaggacg
    27481 tgtgttagat tctactggcg gattagcatt gaaagaagta cctaagaaat ttgtgatcat
    27541 cggtggcggt gtgattggtg ccgaattggg cggagcgtac gcaaacttag gagcagaagt
    27601 aacgattctt gaaggctcac cacaaatttt accaacttac gaaaaagatc tagtaaaatt
    27661 agtagaagat gatttcaaga aaaaaggcgt aacagtcgta acgaatgcaa tggcgaaaga
    27721 agcagtcgat aacggtgaca gcgtgacagt gaaatatgca gtggacggga aagaagaatc
    27781 cgttactgct gattatgtga tggtcactgt aggtcgtcgt ccgaatacag atgatatggg
    27841 cttagaacaa gcgggtgtcg aagtaggaga acgaggattg atcacggtgg ataaacaagg
    27901 acgtacgaac gtacctaaca tctatgccat cggtgatatc gttccagggg cggcattagc
    27961 acacaaagcc agttatgaag caaaaatcgc tgctgaagca atctctggca aaaaagtagc
    28021 tgtagattat aaagcaatgc cggcagtagc cttcactgat cctgaattgg cttctgtagg
    28081 tatgacgatc aaagaagcaa aagatgctgg tatcgaagca aaagcatata aattcccatt
    28141 ttcagggaac ggtcgggcat tatcgcttgg taaaacagaa ggattcatcc gtctagttac
    28201 gacgatcgaa gataacgtgc ttattggcgc gcaaatcggt ggagtcggcg caagtgatat
    28261 ggtctctgaa ttagctttag caatcgaatc aggaatgaat gctgaagata tcgccttgac
    28321 gattcatcct catccatctc ttggcgaaat cgtcatggat gcttcggaat tagcattagg
    28381 tttaccaatc catatttgat ttgaataata caggcgaaac gcaactgaaa aatcagttgc
    28441 gttttgtttt ttagaattat caataatatt attatgtaaa cttaaaaata tgacaacttc
    28501 ttggtttatt accacttcct atgctacact gaagaggaat atgaaatagg agagttgaag
    28561 atgcaagaat atcagtatcc acttgattta gattggacaa cagacgaaat ggtcattgta
    28621 atgaagatgt gggaagatct ggaacgtgcg aatgaaaaag ggataaacaa tgaagctttt
    28681 ttgaagactt atcagcagtt taaaactgtt gtcaaatcca ttggtgagga aaaacgattg
    28741 ggacgggaat ttgaaaaagc ttccggttat tctttatatc gtacagtgca gcaagcaaaa
    28801 aaaaatccaa acaaaacttt gagaatgaac gggtgagata aatggaacag atgattgatc
    28861 atatcaagtc ctggcttgaa gaagctggtg agaaaatcaa atcttctcaa caaacattaa
    28921 caatctcgca aaaatcaaat cgaacggatc tagtaacaaa tgtcgatcga aacatccaga
    28981 attttctaat cgaaaaaatc cattcctttg atccagaggc gaaaatattg gcagaagaag
    29041 aagggtgtaa tcaattatcg gatctcgatg gaagagtctt tatcatcgat cctattgatg
    29101 gcaccttgaa ttttgtgatt gaaggggaaa atttttgtat tatgctcgct gtttatgaat
    29161 ccggcgttgg aaagcttggt ttcatttatg acgtgatgag aaatgaactt ttttggggtg
    29221 gaaaaggaat aggcgtgtat aaaaatgatg agccattgcc tagaccagca aataaatcct
    29281 tatcggaagg cttacttggt gtgaatagtt atcttttcgg tcataataaa tatggaatcc
    29341 gagaaattgc ggaaagcagc atggggacac gtatgtacgg atgcgctggt cttgaactag
    29401 tggcaatctt gaaaggaaac cacattgctt atatttcaaa ccttagccct tgggattatg
    29461 cagcgggaaa tgtcttattg gaagagtttg gtatggtaac aagcggtctt tttggtactc
    29521 ctttgacatt tgaaaaaagg gaatactatt tagcagggac ttcttgtgca tatgcagaaa
    29581 tcgcagacct tttgaaaaaa gaaggctgaa aaaaaggtag aaatcattca caaaaatgat
    29641 aaaaacgagc aaaagattaa ttttctcgaa aaaatgaaaa cattttacta tatttcatga
    29701 aaaaagtctg ctataattag cagtcgagta aaatttgccc tgtcataaag aaccaataaa
    29761 aaaggagtta gttacattga attatagaaa cgatatccgt aacgtcgcaa ttattgccca
    29821 cgttgaccac ggaaaaacaa cattagttga tgaactatta aaacaatcag atacattaga
    29881 cgcacatacg caattgcaag aacgtgcgat ggactcaaat gcgctagaaa aagaacgtgg
    29941 tatcacgatt ttagccaaaa acacagctgt agactataaa ggtattcgcg taaacatcat
    30001 ggatactcca ggacacgcgg acttcggtgg cgaagtagaa cggatcatga aaatggttga
    30061 cggcgttgtg ttggtcgtag atgcgtatga aggaacaatg cctcaaacac gttttgtatt
    30121 gaaaaaagca ctggaacaac atatcacacc aatcgttgtc gtaaacaaga ttgacaaacc
    30181 ttctgctcgt cctgagcatg tcgtagatga agttcttgaa ctattcatcg aattaggagc
    30241 agatgatgat cagttagact ttcctgtgat ttatgcatct gcactaaatg gtacgtcaag
    30301 cttgtcagat gatccagcag atcaagaacc aaccatggca cctatttttg atacaattat
    30361 tgaaaaaatc cctgctcctg tcgataacag cgacgaacca ttacaattcc aagtttcatt
    30421 acttgactac aacgattatg taggtcgtat cggaatcgga cgtgtattta gaggaacaat
    30481 caaagtagga gaccaagtag cattaattaa attagacgga acagttaaga aattccgtgt
    30541 aacgaaatta tttggtttct ttggactaaa acgtctagag attcaagaag caaaagctgg
    30601 agacttgatt gctgtctcag gtatggaaga tatctttgtc ggtgaaacag ttactcctgt
    30661 ggatcatcaa gatgcgttac ctatccttca cattgacgaa ccaacattgc aaatgacttt
    30721 cttagttaat aattcaccat ttgccggtcg agaaggtaaa ttcgtaaccg ctcgtaagat
    30781 tgaagaacgt ttgatggcag aacttcaaac agatgtatcc ttacgagttg aaccaacaaa
    30841 ctctccagat gcatggacag tttctggtcg tggagaattg catttgtcta tcttgattga
    30901 aaacatgcgc cgtgagggat acgaattgca agtatctcgt ccagaagtta ttgaaaaaga
    30961 aatcgacggt gtaaaatgcg aaccgtttga acgcgtgcaa atcgatacac cagaagaata
    31021 tatgggtagt gttatcgagt ccttgagctt acgtaaaggt gaaatgcaag atatggtcca
    31081 tacaggaaac ggacaaatcc gtttgacatt cttaacacct gcccgcggtt tgattggtta
    31141 ttcaactgaa ttcctttcaa tgacacgtgg ttacggaatc atgaaccata cttttgacca
    31201 atatctgcca atgcttcctg gtcaaattgg cggacgtcac caaggggcgc ttgtttcgat
    31261 cgatacagga aaagcaacga cttattctat catgagtatc gaagaacgcg gtacagtctt
    31321 cgtagaacca ggtacagaag tatatgaagg aatgattatc ggggaaaaca gccgtgataa
    31381 tgatttgact gtcaacatca cgaaagcaaa acaaatgaca aacgtacggt ctgctactaa
    31441 agaccaaaca tctgtcatca aaaaaccaaa acaattgacg ttggaagaat ctttggaatt
    31501 cttgaatgat gatgaatatt gcgaagtaac accagaaagc attcgcttga gaaaacaaat
    31561 tttagagaaa aatgctcgtg aaaaagcatc taaaaagaaa aaataatctg taaaaggatg
    31621 aagagagaaa ccagcttgtt
//
LOCUS       DAGCYL010000013        31441 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.013, whole genome shotgun sequence.
ACCESSION   DAGCYL010000013 DAGCYL010000000
VERSION     DAGCYL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.013"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(449..2341)
                     /locus_tag="MB216_000444"
     CDS             complement(449..2341)
                     /locus_tag="MB216_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4525305.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2338..4065)
                     /locus_tag="MB216_000445"
     CDS             complement(2338..4065)
                     /locus_tag="MB216_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4525306.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4081..4731)
                     /locus_tag="MB216_000446"
     CDS             complement(4081..4731)
                     /locus_tag="MB216_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBT4525307.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4935..5231
                     /locus_tag="MB216_000447"
     CDS             4935..5231
                     /locus_tag="MB216_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBT4525308.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5423..6043
                     /locus_tag="MB216_000448"
     CDS             5423..6043
                     /locus_tag="MB216_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBT4525309.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6135..7301)
                     /locus_tag="MB216_000449"
     CDS             complement(6135..7301)
                     /locus_tag="MB216_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4525310.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7313..7666)
                     /locus_tag="MB216_000450"
     CDS             complement(7313..7666)
                     /locus_tag="MB216_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBT4525311.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7794..8816
                     /locus_tag="MB216_000451"
     CDS             7794..8816
                     /locus_tag="MB216_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBT4525312.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8835..8996
                     /locus_tag="MB216_000452"
     CDS             8835..8996
                     /locus_tag="MB216_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525313.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9255..9689)
                     /locus_tag="MB216_000453"
     CDS             complement(9255..9689)
                     /locus_tag="MB216_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525314.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9799..11658)
                     /locus_tag="MB216_000454"
     CDS             complement(9799..11658)
                     /locus_tag="MB216_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBT4525315.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11776..12935)
                     /locus_tag="MB216_000455"
                     /pseudo
     CDS             complement(11776..12935)
                     /locus_tag="MB216_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(13242..14666)
                     /gene="araA"
                     /locus_tag="MB216_000456"
     CDS             complement(13242..14666)
                     /gene="araA"
                     /locus_tag="MB216_000456"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBT4525316.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14682..15380)
                     /locus_tag="MB216_000457"
     CDS             complement(14682..15380)
                     /locus_tag="MB216_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBT4525317.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15337..16998)
                     /locus_tag="MB216_000458"
     CDS             complement(15337..16998)
                     /locus_tag="MB216_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBT4525318.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17216..18616
                     /locus_tag="MB216_000459"
     CDS             17216..18616
                     /locus_tag="MB216_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBT4525319.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18868..19947)
                     /locus_tag="MB216_000460"
     CDS             complement(18868..19947)
                     /locus_tag="MB216_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4525320.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20033..20992)
                     /locus_tag="MB216_000461"
     CDS             complement(20033..20992)
                     /locus_tag="MB216_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBT4525321.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(21004..22515)
                     /locus_tag="MB216_000462"
     CDS             complement(21004..22515)
                     /locus_tag="MB216_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBT4525322.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22537..23100)
                     /locus_tag="MB216_000463"
     CDS             complement(22537..23100)
                     /locus_tag="MB216_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBT4525323.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(23106..23969)
                     /locus_tag="MB216_000464"
     CDS             complement(23106..23969)
                     /locus_tag="MB216_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4525324.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23983..24879)
                     /locus_tag="MB216_000465"
     CDS             complement(23983..24879)
                     /locus_tag="MB216_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4525325.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(25054..26343)
                     /locus_tag="MB216_000466"
     CDS             complement(25054..26343)
                     /locus_tag="MB216_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4525326.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26315..27832)
                     /locus_tag="MB216_000467"
     CDS             complement(26315..27832)
                     /locus_tag="MB216_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBT4525327.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            28030..28920
                     /locus_tag="MB216_000468"
     CDS             28030..28920
                     /locus_tag="MB216_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBT4525328.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28993..29817)
                     /gene="nadE"
                     /locus_tag="MB216_000469"
     CDS             complement(28993..29817)
                     /gene="nadE"
                     /locus_tag="MB216_000469"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBT4525329.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29829..31301)
                     /locus_tag="MB216_000470"
     CDS             complement(29829..31301)
                     /locus_tag="MB216_000470"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBT4525330.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         9172 a         6602 c         5467 g        10200 t
ORIGIN      
        1 gaacaccgtt taaggaagag gttgtgaaaa agtcgcttag tttcgagtag taagaaaaat
       61 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
      121 tttgaacacc gtttaccaag aggttgtgag cctgccgttt aactccgagt cacaaggagc
      181 aatttcaaaa aacagcttct caggtttgtt gactattgtg acttgtgacc gaggagttgg
      241 caggagaaca ccgtttaagg aagaggttgt aaaatcagcg gtttgacctg agtattagag
      301 atacaaaacg gaaaaatagc ttctcatatt ttgccgtttt ttgagctaat accgaaggac
      361 agctgattga acaccgttta ttcggaaaaa gggactgtga cacgactttt gtcacagccc
      421 ctaaaaaaaa taataaattt gtactattct atgaagcttc ttcttcagaa aactgactgt
      481 tataaagatc tgcatagaat ccgtcttttt ccatcaatgt atcgtggttt cctgtttcta
      541 cgatcgaacc atgattcatc acaataatgt tgtctgcatc gcgtattgta gacaagcggt
      601 gagcaacaac gaaacttgtc cggttttcca ataggcggtt catcgctttt tgaatcagta
      661 tttctgttcg tgtatctaca ctagatgttg cttcatcaag gatcaggaca tctggatttg
      721 ctaagaatgc ccgcgcaatt gtgattagct gacgctgccc ttgcgaaata ttgcttgcat
      781 cctcgtttaa tacagtgtta tatccttctg gcaatttacg aacaaaatcg tctacgtgag
      841 cagctttagc agcctcaatg atttgttcgt ctgttgcatc gtcgttccca tatttgatat
      901 tgtcatagat acttcctgta aacagccaag tatcttgcag taccattgaa aactgtgcgc
      961 gcaattcatc acgtgaaaga tcacgggtat ctacaccatc gtaacgaata ctacctccag
     1021 aaacatcgta gaaccgttca agaaggttga tcagtgtcgt cttccctgca ccagtcggac
     1081 ctacaatagc gaccatctca ccaggtttga catccaagtt aaaatcagtc attagcaagt
     1141 tatctggtgt gtagccgaat tgaacatttt caaaacgaac tttgtatggt gtatcttttt
     1201 cttctggaag gccagatttt tcattggaca tctcttcttc atccaacact tcaaataccc
     1261 gttcggctga agcaactgtt gattggatag tgttcatcaa acttgcaagt tgagtgattg
     1321 gttgagagaa ctgtgttgta tattgcaaga acgcttgaac atcccctaat gtcatgttcc
     1381 catttgcaac ttttactccg cctagtacag cgacgaacac atagcctaga tttttaacga
     1441 aattcatcaa cggcatgatg attgccgaaa taaattgagc tttccaacca gcggcataca
     1501 gattctgatt ttctttttca aatacttcct ggtctttttc agtatgattg aatgttttaa
     1561 caatggtatg accaccatat gtttcttcta cttggttatt caataaacct aaactttttt
     1621 gctgatctgc gaaaaacact tgtgatttcg gcgctacgat catcacgatg atcaagctta
     1681 aagggatcat caataacgcg actaacgtca attgccagct gattgaaagc atcatcacga
     1741 tgaccccaat caatgtgact gcactagtta caaattgagt caaattttgc tgcagcgtgc
     1801 ttgcgatgtt atccatatca ttgattgctc gagacatgat atccccattt gtatgtgtat
     1861 cataatagga aatcggtaca cgattcattt tggcttccaa atcatgacgt aattcataaa
     1921 ctgttcgttg ggaaacacgt gtcataataa attgctgtaa aaagttgaat acagcagaaa
     1981 tcaaatacat cacgattact gtcgcaatga tctgcgcgat tttatcaaag tctatcggaa
     2041 attttgttac ttgttggcct tgttgcatct gttgagcgcc agccattaca cctttgaata
     2101 tttctgtggt tgctttcccc aagacctttg gtgtttggat ttggaagatc acggctgcta
     2161 ttgctagtac aaatacagcg atgatcgcaa ccatacgttt agacatatag ccaaacaatc
     2221 ggcgtaccgt tccccagaaa tttttaggct tttcaccttt tacaccgaga tttctgccag
     2281 gtccaccatg tcctggaccg cctgctggtc catgagaggg acgtgtgttt gttttactca
     2341 tgctaagtcc tcctctctta gttgtgaatg aacgatttct tgataagttt cgtttgttgc
     2401 taatagttct tcatgtgttc ctttgccaac cagttttcct tcatctagca caaggatcat
     2461 atctgcatcc attactgtac ttactcgttg agcaactaaa acggtgatac tttctttcat
     2521 attcgttttc aatgcttggc gaagattggc atccgttttg aaatccaacg ctgagaagga
     2581 atcatcaaac acatagatat ctgctgtttt gactaatgcc cgtgcaattg ccagacgctg
     2641 tctttgtcca ccagagaagt ttcctcctcc ttgttccaca tggctatcta agccatcttc
     2701 catctctgaa acaaaatctt tagcttgtgc gatttctaat gctttccaaa tcatttcatc
     2761 tgttgcatcc ggcttaccat actgcatatt gtctcggatc gttccagaga acagaactgc
     2821 tttttgagga acaaagccca ttaattctcg caaattatat tgagtcatgt cactaatgtc
     2881 cgttccattt atttggatcg aaccagattc gatgtcatat aatcgaggga tcaaattgac
     2941 taaggtcgtt ttacctgaac cagtcccccc tatgattgcg acgatctcgc ccgacttggc
     3001 ttggaaatca atatcttcca atgcaagttt ttctgctcca tgatatcgat aattgacatg
     3061 attgaaggcc aatgttgcgt tttttccagc aaaagaaact gttttaggat tttcagggtc
     3121 atggataccg attttttcat ccaacacttc attaatacgg tcagctgaag cttgtgcacg
     3181 aggcaccatg ataaaaatag cggaaagcat cataaagctg atcaagatct gcatcgcata
     3241 agtcataaat gcaatcagat taccgacttc caaagtcata tctgctatgt agtgtccacc
     3301 aaaccaagtg attgctacgt ttgtaccgct catgatcaat gtcatcattg gcatcatcaa
     3361 tgcgacgatc gtattaactt tgattgccgt atttgtataa tctttattcg ctaaatcaaa
     3421 acgattttct tcaaagcgtg tcttatcgaa tgctcgaatc actctgaccc ctgtcaatcc
     3481 ttctcgaaat accaggttta aacggtcagt tttcttttgc atacttttga ataatggaac
     3541 agcaaagtac atgattccgc cgatcaaaat aatcattact gggatgacaa ctaagaagat
     3601 ttttgtcaac tgatgatctt tttgataagc aagtatgctc gcgccaatca acgtgattgg
     3661 tgcattgatc atcatacgta aaaacatttg tgtaaccatt tggatttgag tcacatcatt
     3721 ggtcgttctt gtgatcaaag aggccgttcc atatttatca aacgcatctt ttgtcagact
     3781 ctctgatttt ttatagacat ccgtacgcaa ttgcttaccc agtttctgtg attcacgagt
     3841 agcaaaaaat gtatttgcga tagcagctaa gatactgatc aaagaaaagc cgatcatcac
     3901 aaaacctgta tgccaaatat aatcgacatc tccttgcgct acccctttat caataatatt
     3961 ggaggtaagt gtcggcaaat ataaatctgc cagtacttgg atcaccatga aaactgcagc
     4021 tgcaattgct gaagtcaagg agatccgttt tacgattttt agcatctagt tcccttcttt
     4081 ctatttatat actccgtttt tcagccaaga aagtttcaaa ttgaattcac tgattgcttg
     4141 ctctactgag taatcttcag atgttgaaaa taagcgatac gaatgcgcaa ttgttgtaaa
     4201 tacagtattc atcactaatt ggatcagtaa ttccaattca tgatcatttt ttactttcag
     4261 cttcgtccga tcgatcaatt caagcaactc ttttgcatct tgtttgtgtt ctttatgtgg
     4321 atcgtgaggc gttttttggc gactcgtatg aggagcgact tttcttgagg cacggtaatc
     4381 catgttcata aacaagttac gataaaaaga agcattctcg ccttccaaaa tctcataaat
     4441 cattttagaa aaataggatt cgaatccttt gaatagatca ccatcggatt ctttcattag
     4501 ttgtaacata tctttagagc tgtcacgtct cattgtctca aagtaataaa aataaagatc
     4561 ctctttatct tcaaaatact gatagaaact gcctctaggg atatccgcta atttgacgat
     4621 attagcaatc gaagcttctt ttaagggtac tctagaaaac tcgattctcg cagcctcgat
     4681 tagtcttttt tgtttttctt taggcaaatg gaaaaatgtc tgtgtcggca tatggattct
     4741 cctttctttc aagatgacac attgtcatat gacgctatgt cattataaca cctcctacct
     4801 gttttgcaag acatttgcat agtttttttc attatcatcc gatacattaa aaaagtgata
     4861 agaggaatcc ttccatttga attaatgaga gttgcccatt aaaatagcca catactaaac
     4921 aaaggatgag ataaatggcc caatcaatta gtgtccaaga cttcaataaa ttgcctctcg
     4981 atgctgacag catcgtttta gatacaagaa ataccttaga ttacacaaaa aaccacttaa
     5041 gccactcgct ttctatcccc gctgctatct tgccaagaca attaaaaaat ctagatccaa
     5101 agaaacttta ttatattcta agctatacag gcaggcgatc agaagcatta gccgatcaac
     5161 ttactgctaa tggttttcgt gctgtttata tcattggtgg gatggaagct cttcagtcga
     5221 gtgttgcttg aatattgctt gttagattca aaagaggctg tgacaaaaaa tcgtgtcaca
     5281 gcctctttta taccctgtct ttttttagtt atttattctt caaccttatc aatatgactt
     5341 gatagccttt tctttttcga gtaaactgat tttgttagtc atagttataa caatcatagc
     5401 aataaaagga gaaggtaagc atatggcatc tgttttagta gtcaaaggtc atcccctaac
     5461 tgcagaagaa tcgcgcacag ttaaggcatt gacgtctttt ttaacaagtt ataaagaaaa
     5521 tcatccagat gatgaagtga ctgttttgga actttatcgc gatgatattc cggaaatcga
     5581 tgaagaatta ttatcaggtt gggaagcatt acgtgctggt gcagaattca cttccttatc
     5641 agaaagtcaa caacaaaaaa ttgctcgttt caatgaattg actgatcagt tccttgctgc
     5701 tgataaaatc gttatcgcaa acgccttatg gaatctaaat atcccaacaa aattaaaagc
     5761 ttggttcgat accgtcaatg tagctggaaa aacgttccgc tatacagaaa acgggccaga
     5821 aggattagtg acaggtaaaa aagcactgca catccaatct aatggcggtg tatacaacgg
     5881 acaagacttt gcctctcaat atgtaaaagg tattttaaac tttgtaggca tcgatcaagt
     5941 agatcagcta ttcatcgaag gaatcgatta tgaccctgac cgtgcagatg agttgatgca
     6001 aaatgcatta gataaagctg ctgctttagg taaatcattt taaaaattga gataaatgca
     6061 tacaaaagag acagaggaaa aaatcttcaa agcagcattt gtgccttgga tattttccca
     6121 tctgtctctt tttgttattc tttctgcttt ttcaacggat tttcttcata aagagaagag
     6181 attgttgtgt accattcaaa aacatgagta atcactactt tagctgctgc ataaacagga
     6241 atccccaaga tcacaccgat gattccgaac aatttcccag aagtcagcag aactaataaa
     6301 atcgttactg gatggattgc taactgattc cctagtacta atggagagat caagcgtcct
     6361 tctattgtct gctctactgc aaaaacgatc agtactttga tgatcatgaa tggacctgct
     6421 acaatggcta aaaagatagc cggaatcatc gctaaaaatg atcctaagta agggatcagg
     6481 tttaaaaaac ccgcaataat ccccaacgtg acagcatact ctaaaccaat gaccgcaaaa
     6541 ccaatcataa acatgattgc cacagcaaaa gccacagtta gttgtcctct tatataagaa
     6601 gaaacttgac tattcatttc actcagcaca ttcaacgtcg gtttttgcat gcgtgtcggt
     6661 aaaaatttga caaaataagg tgctaattgt ttaccgtctt tcaatagata aaataagata
     6721 aacggcatcg tcagcagagc cactaaaata gtcgctaccg cgccgacaaa acttccaaga
     6781 ctctgaactg tactcttaga tatatcttgg atcatatcgc ctgccgaatt catgaactta
     6841 tcacctgctg tttccaactg ttcgcgaaat tggttgaaca gcgggtcccg caaaatctcg
     6901 gttaatttat tatcaatcgt atctacatat tgagggaagt tactgatgaa gctcactgtt
     6961 tgttcctgaa tcttagggat gatcactacg ctcccccatg cgattagtgc aacaaccaaa
     7021 acaaacagcc caatgatact gtaaagcctt ggtacttttc gcttttcaaa gtaatcaact
     7081 accggattca tcaagtaata caagattcct gctaaaatga taggcagacc tacgattgct
     7141 aaaaattgcc agattggtgc aaacaaatat gaaacttttg taaataaaaa gacgatcagc
     7201 aatattaaca atacaacaag taaggctgtg actacttgat tatttaaaaa ccagcgccaa
     7261 aaccacgata ccggtctgtt cttcttattt tttccttcca taattcctcc tgttagttat
     7321 aaaaaatttg gcttcccaaa ccaatagcag gcagtctatg cggatgaaga tagattccat
     7381 ttgcaatttg tccttcttct ggacaagcat caaaaatcaa tgatacgtga gctactgtct
     7441 ttaaattccc attggccatc ggaccgacat aatcgatcgt atatacctga tcatcaaaag
     7501 caattgttcc gcctgctttc aaagaaaaag gttcgtcttt tgatacttct tgaatcaccg
     7561 aatattttcg taaggcagct gttgctgttt tatcgaacaa aatcagcatg ggttcttcct
     7621 tgtttattgc ttctgtccca atttctgtaa tctttgcctg aatcattgca ctctctcctt
     7681 taattgcttt caaaaacagt ataacacatt ctcattcttt agccgtgtgg aataattatg
     7741 tgatagaata aaaacgaaaa ctatttcatt acaattagga ggaatcagct catatgatcg
     7801 aaaagataat tttaggaacc tatacgcgtc gcgaaagtaa aggaatctat acgatcgacc
     7861 ttgatacaga aaaagaagaa ttagccaacc tttcattagc tgttgaagaa aatagcccta
     7921 cctatgtagc aaaaagtaaa gctggcaatc tttacacagt tactagtgtg gatggattag
     7981 gtggtgccgg tgcgtacgat aaggattttc attttttgaa tgcggtaaca gagtctggcg
     8041 ctcctttgtg ttatgtagcg gttgatgaag cacgtcaatt actatacggg gcgaactatc
     8101 ataaaggtga agtaaatgtc taccatatat taggagatgg cgaactgaaa gcagcagatt
     8161 caatattcca tcaagaagcg acaggtcctc ataaaaacca agatcatgcg catgtccact
     8221 atacggatct gactcctgac caacgattag tcgtatgtga tcttggtaca gatcgtgtgt
     8281 atacttatga tgtgtccgaa aacggcaaat tgaatcttgt aactaccttc actgcagagc
     8341 caggaacagg ccctcgccat cttgtctttc atccaaatgg acaattcgct tatttatttg
     8401 gtgaattaga cagcacagtc cgcgtgcttt cttataataa agaaacaggt gcttttgaag
     8461 aattacagaa aatatcgacc ttgccagaag aattcgacgg tgaaaatgga ggggcagcta
     8521 tccgcatttc aaatgatggc aagttccttt atgcttctaa ccgaggacat aactcgattg
     8581 ctgtattcgc agtttttgaa gatggttccc acattcaaaa caagcaaatc atctcaacag
     8641 aaggggattt tccccgcgat ttcgcactag atccaagcaa taattttgtc gtgtgtgcca
     8701 atcagaacac cgataatctg acattatatc gccgtcatcc tgaaacagga ttactggaat
     8761 tactgaaaaa agatgtctat gtaccagaat gtgtctgtgt ttattttgat cgttagtaag
     8821 aaaaggagaa tgaaatgaat cagcaagacg tattagacag actacgggaa gaactaaata
     8881 ttcctttctt tgatgggaaa atcgaagata aagagtatag cgaagaggaa tatcagaaac
     8941 tcaaagccga tttacagaac tactttgaac aatatgtaca gaatgtagaa aactaatcag
     9001 ctttccatac tatctgaaaa gaacatcagg acaagacttt tccgtctatc ctgatgttct
     9061 tttctacaaa taaaaaaccg ccgagatggt tagactcgac ggaaaaggag tatttactcg
     9121 attttgagta agtacaaggt tgttgttggt tataggtatg tcattatcat aaaggatttt
     9181 gatcactcta tcataaaggt aatattacat ttatattaga taactctagt ttttattgtt
     9241 atcaaagatt tcttttattt tttcaacaac aaattagtca ttttttggat ggcagctgtc
     9301 aattggtctg tcgcttggtc ttgttttgct tcagatgtat tgaatagatc ttcatctacc
     9361 cctgattcaa gatcgatact ttggatcatt tcttcacagc ctgctacata tgaaagataa
     9421 gctttttcaa aatttttatg aatgcccatt gcttttgctg gtggacgcaa agtactgact
     9481 ttcataagca ttgcccgata tttttttgtt ccttcttcaa ataacaggat gatctcttca
     9541 agagtcgatt tgtccagttc acttgttttt ccttcatcaa ttgcgtttct gatcacttca
     9601 tatttcggat gcattccttc tccgattgct tctgtttcct tgacgatttg atcgatggac
     9661 tgcacataat agccaatatt cggtaacatg ttcgttttcc tactttctcc attgttgtta
     9721 ttatttattc tacttgtttt tatcctcaaa tgacaagtac ttgaaaaaat ttaatggttt
     9781 ctatacagct cgccattctt attcgtgctc aaagaaacca tctgctaaac tatattgttt
     9841 ccctgcgaca atgtcatatt ggatcaattg atattcgtcg taaatctctt gttgcgagcg
     9901 attgaattgc gcttctttat accactgtcc gttttgatag tacagttctc gttcaaacgc
     9961 cggaagttcc tgttctaatg ataaaagcag ctgataaaag ccatttgttg tctgattcgt
    10021 ctgttccatt agattcgctg caaaataaaa cggactggtg attgcatcgt gagtcgtttg
    10081 cttttctaat tctcctttac tgtcgaacat aagaaattgt gtttcatgca atgtttgttt
    10141 gtcatttttt gcttggatcg tatctgaata gatccctgga agatgatccc cccaaaaaac
    10201 aactaaagtc cttctagaaa gattcttcaa ttcttccgtg aactgcttca gtgccgtgct
    10261 gctataggaa atatcttgca gataattctc taagtcaagt gtcccacttc catctgaagt
    10321 ttttgcagga tagtctagat tatcatactt tccatcgtag ggcatatgtg tctgcatcgt
    10381 gactaaatga atgaactgag gtgttttttc ttccttgaga agtgtcatta cttctttgta
    10441 agctgactca tcagaaatat atggattatt ctctatcgta tccgtatagg tcatcgtacg
    10501 ctcgctgatg aactggtcaa atcctaatgt ctgataaaca tcctctcttt tatacatcga
    10561 agtattgtat ggatggatcg ctgtcgtctg gtatgattgt gcgtttaacg ctgaaacgat
    10621 agaaggaagc tgatccattt ttggtacaag catcgtgtaa ggggtggtga gttgcgcatt
    10681 aaataacgcc atagaaaaac cagtcaatgc ttcaaattcg atatttgctg ttccaccgcc
    10741 atagttttga gaaagcatat tgccactata tgtttgatca gctacttcat aataatctgc
    10801 caatggctca ccagatactt ctacgccgtt taatcgacta ggatctgaga aactttcact
    10861 catcacgaac acgatgttcg gcgactcttc ctctacagtt ttattttttt catcggctag
    10921 cttctggtat ttttcagtga tttctttgat cttttctttc gaatatcctt caggttcatc
    10981 cattggttct accttcagat tgtacaaaaa cccaccgata aaaccagtgt tatagtagtt
    11041 cattttctgg ctgtaaggta tccatagtgc cgttttatta tatgcttttc gcaacaaatt
    11101 atcgggattg ttgaaattac tgatataccc tagcagtcca attgccacaa gcagtgtcgt
    11161 taatcggatc aattggaatc gccttccttt tttcaggctt cgaaacatgt accagcaaaa
    11221 caatcctatc acggttcctg cggcagctaa gatcactgta aacagcatag tgccggtcat
    11281 atctttcagc aatccgattt cagtgatcat cttcaaatcg tctggataaa tcggttcttg
    11341 ccgataactc attttcatat aatttgcaaa acctaataca ccgatggcta cgacatacgt
    11401 aagggaacct aaagggacag atcctgctag tgcaatcaga aaaataagaa taatcagcaa
    11461 gacgagacag gctaagaaga atttttccgt atgccaagaa aaagcaaatt tcaacgcaag
    11521 gtctacggat agatcatttt gacaccactg caaaaataaa ttactaaaaa caacaagaaa
    11581 aaagatactg atgattgctg ctgttccttt taaaatcttt ttctgtttgg tcgaaagtct
    11641 gcttatatat cttttcaaat gcttgctcct tcatttctat caataaaaga atgttgcttc
    11701 tcttatttta gttgtacgaa attcgaaagt aaagaacgga aagaagaatt aatgaaatcc
    11761 aaatattctc tttctttatg gcctttctgg tttctttaat aagcggacct ttcgtactcc
    11821 agaaattttt tctagctgtt gtttcacttg attgagctgt tcaaaatcag tactgtctat
    11881 gtcaatcaat atgtagacat gctcattctt atggttacgc gatatatctg cgatcgctag
    11941 ctcgttctca ttcagcagag aaaatatctc tgctaatata ttggttttct tttgataaaa
    12001 tatagtgaat cgaaacggcg cttggaaaaa cattcgtgcg gatggataat tgaccgcttc
    12061 tctaaccgta ccgaacaaaa gataatcttt taaggcttca actgccaaac gtccgctgtc
    12121 tgccaaagct tcctgagtcg atcccctaga tgaggcaaca taagtatacg gtcattttct
    12181 aagaactctt cttctggaaa atcagtcaag tatttagcca gatgttgttt ttccaatgct
    12241 tgcataacag catatttatc aacaatttca cttcttccta cattgataag tacagcatct
    12301 tttttcattt tttctatatt cgcctgatcg atcaatcctt ttgtatcttc tgtcaacggc
    12361 agcatgacta cgatgtaatc cgaggtagaa agaactgttt ctaaatctgc ttgccgaaca
    12421 taatccaact gggcatcgcg gatagaataa cccaagacat ccatccctaa gctatagcaa
    12481 cttagcgcta cttttgtgcc tatcgctcca agaccaagaa gaccgatggt tttgccttgc
    12541 aattctcgtc ccacataagc acttcttttg ttttctgcct gttctaaaat attaggacct
    12601 gttagtgttt gtaccatacg acttgcttcg attatcggac gggaagataa gagcaaacag
    12661 cacaaaacaa gctcttttac tgcattagca tttacacctg gtgtattcat gacgatcgtc
    12721 ccgttttcag aagctttttc aacgttgatc gtgttcaccc caacaccagc tcttgaaatt
    12781 gctagcaaat ctggtgtaag ccattcatct ttcactttcg tactacgaat aactattcct
    12841 tcggctggca aatcatctat tattttgaaa tattcccctt gtaacttttc tatctcttgt
    12901 tctggaatag catcaatcaa atgaacgtga tacataaaac cccctcctta ctaacaagta
    12961 tctcgaaatt tctgcaatta gacaactgtt taccatttga cttcctagaa gcaaaaagag
    13021 cagttagagc agagtaggct gagtgataaa aataaggcta tgacaagtta ctgccataag
    13081 ccttattttc gagagcaaaa tattgagggt gagatatctt gataaatggt gttccgttag
    13141 ctgacattga taataagact aaatttcaaa aaagttgaaa agcaaatttc gtcaatctct
    13201 tcttatttct taaaactaag tggctttttc acaacctctt tttatctaat aaccactgtt
    13261 tccaactcaa ctaattttgc ccacgtttca atttgatctg ttgttagatt tagcgagaca
    13321 acggtatgat gaccgccacc tgcctgaatc catgaatgga caccttcatg gaaattcggt
    13381 ttagttttcc ataacacacg tgcaacaggt aaattaggtg cttcagtcgt tggttcaaag
    13441 gcttctactt cattgatcag cagtttataa tgcgtaccga aatctgccat cgatacgact
    13501 accccttctc ctgctttccc atcaaataca agacgcgccg gatcttctct atctcccatt
    13561 gataaaggag aaacaacgat tcttggtttc gtcgcagcaa gcgtcggatc tacttccatc
    13621 atatgggatt ccaagattgc ttcttggcca ctagctagct cgtaagtata atcttccatg
    13681 aatcctgtat ctttccctcg agccatgatt ttcagcaacc gatcaatcgc tgcagttttc
    13741 cagtcgcctt ccccagcaaa accgtatcct tctgccatca aacgttgtac agctagtcca
    13801 ggtagttgtt tcattccata caggtcttca aaattagtcg taaaagcgtt atagcctcct
    13861 tcttctaaga aacggcgaat accgatttcc tgctgagctt gtacttttac atgttcctcc
    13921 catgtctttt cttcataatc accatagtcg aaggtataca attccttgta ctcttcaaat
    13981 aattgatcga cttcttcgtc tgatacgcga tcgattactt gtacaagatc tccgatacca
    14041 aaataatcaa cagtccaacc aaattgaatc tgtgcttcca ctttgtctcc ttctgtgaca
    14101 cccacgtttc tcatgttgtc accaaaacga gccactttta ttccaaaact ttcattatag
    14161 gctactgcaa catccatcca atcagcaatc tctttttgga tctcaggtcg tttccagtag
    14221 ccaacgacga ttttattttg tttatttaat cgagcattga tgaacccata ttcgcggtca
    14281 ccatgagcgg attgatttaa attcatgaaa tccatatcga tcgttttcca aggaatgctt
    14341 tcgttatatt gcgtagctaa atgaagcaat ggtttttgta gaagctttgt cccgcggatc
    14401 cacatttttg ctggtgagaa agtatgcatc caagtgatca cacctgctac ttcttctcga
    14461 taattgacct ctttcatgat ttttgtgatc gtatcagcag taactgctaa ttcttgaagg
    14521 cggattggat aaggcaattg gccattttca ttcagttcat cgaccatttc ttgtgcatgt
    14581 ttctttactt ctcgcagtgc ttcttcccca tataaatgct gtgatcctac aacaaaccaa
    14641 aattcttttt ctccaatatt caacataatt agccactcct tttatttgtt ttgtccgtaa
    14701 tacgcatttt ttccgtgttt tcgtaaataa tgtttatcta aaatccgttg aggcagctct
    14761 tctgaaaatg aattgagctg tctggtaaat aaattcatct tgcatacttc atctagtacg
    14821 actgcgttca ttaccgcact ttgcgcatct ttcccccaag taaatggacc gtgtccgtgt
    14881 aaaagtaccc ctggaattgc taagggatca attttgcgtt ctttgaatgt ttctatgata
    14941 acttttcctg tttcatattc gtacccgctg tcgatttcct gttgtgtcaa aaatcgagca
    15001 caaggtaccg atccataaaa agtatctgca tgcgtcgtcc ccatcgcagg aacatccaat
    15061 cctgcttgtg cccagatcgt cgcccatgtc gagtgagtat ggacgattcc tccgatttga
    15121 ggaaatgaac gatataaaac agcatgagta ggcgtatccg aagagggatt gagtttacct
    15181 tcaatgatat gattgtccaa gtccacaacg accatgtcgt ctgctgtcat ctcttcataa
    15241 ctgactccgc taggtttgat aacgaaatat cgtgtttcag gatcaaaagc gctcacattt
    15301 ccccatgtat atttgacgag tccgtgttta ggcaagtcaa gatttgcttg gtatacttct
    15361 tctttcagtt gttccagcat tttctatcac tcctttttct tgcaattgat gaatcgctgt
    15421 tttttcaatg tcgatcccct cacggtaacg tctcataaat atctgatagc ctgctatttc
    15481 ttctgctgaa ggtgtgatgg tgattccttg gtcttgtcca aacacgtctt ctgccaagaa
    15541 gttttctaaa gacttcccat gtcgatccat catataggct gctaataacg cgattcccca
    15601 cgctccacct tctccggctg tgtccatcac tgtaactggt gcctccatag cagatgctag
    15661 aatccgctgt ccaacttctg gtgttttaaa aatcccccca tgccccacta aacgatcaat
    15721 ttggacattc tcagatttca atatatccat tccgattctc attgcaccaa aagcactggc
    15781 taaatggatc cgcatgaaat tcgctaaatc aaaacggcta cctggtgtac gtacaaataa
    15841 tggccttcct tcatttactc ctgtaatgtt ttcaccggaa aaataaccat aagaaagtaa
    15901 tcccccgcaa tccgtatcgc cttctagcgc tttgttgaat aataccgtga ataatttatc
    15961 tctgcttatc tccataccca aagaattggt aaattcttca aaaatactga cccaagcatt
    16021 gatatccgaa gagcagttat tcgtatgaac catgcctact agttctcctg aaggtgtagt
    16081 gaccatgtcg atttccggat ggacattttc tagttctttt tctaatacga tcatagcgaa
    16141 ggcggaagta ccagccgaaa tgttccctgt ccctttggct acactgttcg tagcgaccat
    16201 accggttcct gcatcccctt ctggcggaca aaccgggatt cctggtctca aatttcccgt
    16261 agggtcgatc agataagccc ctgattcgct taacttgcca gcttcttctc ctgctactag
    16321 aactttaggc aaaatgtgat ccaagtcaat cgctacgtgc gctttttcga ttaactgacc
    16381 aaaaatattt ttcatttctt gatggtaggt tttcgttgaa ggatcgatag gaaacatgcc
    16441 tgatgcatct cctacgccta gtactttctc accagtaagc tgccaatgga cataacctgc
    16501 aagcgttgtc aaaaaattaa tttctttcac atgttcttct ttattcaaaa tagattgata
    16561 caaatgagcg atactccatc tctgcggaat attgtattga aatttttccg ttaatattcg
    16621 ttctgcttct tcagtgattg tatttcgcca agtacgaaat ggtactagta actgttcctt
    16681 gctatcaaat gctagataac cgtgcatcat tgcactgaac ccaatcgacc cgattgtcgt
    16741 aagattctcg ccatagattt cgaatacttc tgctgccatt tttcggtaac tgacgcgcag
    16801 tcctttccaa atatcttcta atgaataggt ccagatacca ttttctagtt tgttttccca
    16861 ctcataacta ccagcggcaa taggagtaaa agatgaatca atcaatacag ctttgatcct
    16921 agttgatcca aactcaatcc ctaaagatgt acgaccatct atgacatcta acatactttg
    16981 cgcatttact tccttcataa tgatttaact ccctccttct cgtcaaacct gatcaaaccg
    17041 tctttttagt agcgcttaca atgattattg tatacttttg tacgtacatg tcaagcagtt
    17101 atttacaaga atacaataaa aaacacttgt tttgtacgta cataagttat aaaatgaaat
    17161 tgcttacaaa atctgatcaa ccgccttctc gaaattctag aaggagagaa tacttatgaa
    17221 aaaagaaaaa aaagtttcct catcgtttat ttattttttt ggcgcatttg gcggcattct
    17281 atttggatat gatattggtg ttatgaccgg cgccttacct tttttacagc atgattgggg
    17341 tttggcaggt aaagcttctt tgataggatg gatcacgtct tcggtcatgt taggtgcgat
    17401 ccttggcggc tctttatctg gtcttctttc tgataagctt ggacgacgaa aaatgattct
    17461 tctttctgca cttattttta tggctggttc tgtcctttct gcatctgccc ctcacaatgg
    17521 ttcttatttt ctgattgcag caaggattct tctaggttta gctgttggcg ctgcatctgc
    17581 cctagttcct gcttatatgt ctgaaatggc tcctgctaga ctaaggggaa gattgtcagg
    17641 gatcaatcaa gtgatgattg cttctggcat gctcctttcc tatgttgcag attatttgct
    17701 aaaagggttg cctgaaacga tggcttggcg cgtgatgtta gggctcgctg ctgtccccgc
    17761 gctgatttta tttttcggtg ttttagctct accagagtca ccgcgttttc ttatgcaatc
    17821 tggccgcttg gaagaggcaa aaagagtatt gaactatatt cggacaccaa aagaagctga
    17881 acaggaattc gaacaaatcc agctgaatgt caaacaagaa aagacaaccg gaacttcttg
    17941 gcatactttg tttttagaaa aatatcgttc cttagtcatt gccggtattg gtgtagcagt
    18001 attccagcaa tttcaaggag ctaatgctat tttttactat attccgctga tcgtagaaaa
    18061 agcgactggg catgcagcga gcgatgcatt gatgtggccg atcatccaag gaatcatttt
    18121 agttgctggt tctttgctct ttttggttat tgcagacaag ttcaatcggc gaactttatt
    18181 gaaaataggc ggtagcgtga tgggactttc tttcatccta ccagctgttt taggaactgt
    18241 actggatgct catacaaatt cgttgttgat tctcttgttt ttatgtattt atgtcgcatt
    18301 ttattcttgc acatgggctc cactgacatg ggtaatcgta ggcgaaatat tcccactagc
    18361 tgtccgcggg cgagcatctg gcctagcctc ttcttttaat tggattggtt ctttcctagt
    18421 tggcttgctt tttcctgtga tgactgcttc tatgtctcaa gaaatcgttt tcggcatttt
    18481 tgggatcatc tgctttttag gggttttatt tattcaagag atcgtaccag aaactcgtgg
    18541 gaaatcttta gaggaaatcg aacaaagtgc atctaaaaaa acttatccta aacgtatttc
    18601 tatccatcat tcttaaagag gttgtgacag aagtggtcag cttcaagaaa taagaaggaa
    18661 ccgcttagtt tccagtcaca aggaacaatt tcaaaaaaca gcttctcatg tttgatgaat
    18721 attgtgactt gtgaccagtt ggtaggagaa caccgtttag tggagaggtg gtgaaagaaa
    18781 cgttcagcct tgagcgatga caaaagctct cacaaacatt gcgacgagta accgtaggag
    18841 caataagagg cggtaaaaac ccgtctctta tttttcgttt tctttcactg tttcccgttc
    18901 aactagtaca ggttcataga gaatatttgg ctgtgcctgt cctttttcaa tcgttgtgac
    18961 gatccaccgt gcggcatctt gtcccaactt ttcttttgga tgcgtcaatg ttgtcaattt
    19021 tttattccct aaatggctta aagaagaatc gtctgttcct acgatcgata ggtcatctgg
    19081 aatatgatag ccattttctg tcaacttatc tagcattaaa ctagctattt gatcgttata
    19141 gcaaatgatg ccatctaaag cttgttcttc tttcaaccgt tgtactgttt tttcagcgat
    19201 tgtttctcgt gtttccgtgg tgaaagtaat gatatcttcc gtcaaaaaac gcacattata
    19261 ctgttcacac gcgctgataa aacctttcat cctgtatttt ccttgaagat cgtcgatttt
    19321 tgtgatgaac aacagacgct gatgtccttt ttttatcaaa tattctgctg ccataaaacc
    19381 gactttcaca tcatctaaac aaatataaga ggcatttaac tcttcataag cagcattgat
    19441 catcaccatc ggaatgcctt gttctctcaa agcaacataa agtgctaagt ttgggttata
    19501 ctgattactt ttcgtaggct caacgatcaa accatctacc ccataagcaa tcattttctt
    19561 caaacactct ttctccaatt cgtgatcgtt attcgtactg gctaaaagca atgaataccc
    19621 agattctcgt aattcatttt cgattcctcg gataatcgac ggaaaaatat aatcagagag
    19681 atatgtggtg atgacaccga tcgtttttgt tctgttcaat ggtgtttttt ctttcttata
    19741 gctgtcatct acatatgtgc ccgacccttt ttctttacgc agatagccct catttaccaa
    19801 cacagcgatt gcttgccgaa tcgtatgtct gctgacgtca tatatttctt gcagcttgaa
    19861 ttctgaggga atggttgtat ttacttcgta ttcctcacga aggatctttt gttttatctc
    19921 ttctgcaata tcttgatatt ttgccatttg ccttgcccct ttttatttgt acgtacatat
    19981 atcataacga gtctgcaagg tttattcaag caaaaatcaa ggtttcttgt gctcataagg
    20041 catacggaac aggttcacca aattctggaa atccttgctc attccacgta aagcattgcg
    20101 cgttcgcgtg ccgattagga ttgtctaacg gatccccttt ttggttcttc tctggtcgag
    20161 catgatagat caaaatatca aagcacccgt cttctgaaac cgtaaacgag ttgtgaccag
    20221 gaccaaattg cttatttttt tcagaagaaa cgaatactgg ctccgataat ttatgccatg
    20281 attcttttaa taaaagattc ttgtgctcat cagcccacag caggcccatg gcatagtttt
    20341 catctgttgc actaccagaa tacgtaatga atattttccc attgcgagta aggactgctg
    20401 gaccttcatt tactgaaaaa ccccgttttt cccattcata ctcaggtata gaaaggagga
    20461 tctgcttccc ctttaacgtc caaggattat ccatttcgga tagataaaga ttagaattcc
    20521 cagtaatttc aggatcttgt tgggcccaca cataatatag ctttccttga gaagagaaag
    20581 ttgttccgtc taaactgaaa gtttcccttt tcgtttttat ttgtcccttt tctatccaat
    20641 ctgtattcat tggatctata tgctgatttt caagtacgaa catgcggtga tgatgagatt
    20701 catcacgtat agttttatga ttgcttgcag caaagtaaat ataccatttt ccattgatat
    20761 aatggacttc aggtgcccag atcaattcac tcatcggtcc ttcttcatga gcctgccata
    20821 caatcaggct tgcagcatgt tctaattcat tcagtgactt tgcccttctt aattcaatca
    20881 cttggtagcc tggaaccgat cctgtaaaat aataataacc atctgtatgt ttatataccc
    20941 aaggatctgc ccgttgccga acgataggat tattataaag tgtcttcatc ataacaaatt
    21001 tcctcacatt ttctttattc taattacatt ccaagagtat ttttctatcc ctacatttaa
    21061 ttcgtcatgt ttttctattg aaaaatagtt tatttcaggt tttatttttt ctggttcttc
    21121 acgtgtattt ttgtccaaca gatcgctact ggttaatgtg tagtgctctc cttgatctag
    21181 caaattgaaa tcatgttcaa tcgttatctt ttgttgttca tttgtgcgat tgatcaagaa
    21241 aatagtcagc tgattgccgt tatctacgat cacactgtcc acataaggaa cattttttat
    21301 cttttcacaa tcataaacag ggcaaatgat ttgttgtttc aatacttgac ctttagcata
    21361 tttcgataag tgttgcatca cataataagt tggattcctc catgcttgtc ctttagcatc
    21421 tgtcagtatt aaaggtattg tgtttactaa caatgattga caactaatct ttacgcgatc
    21481 tgcgtgtcgt aaaatagcca gccctaatga accaaataat aatgtatctt ccatagtaaa
    21541 gtaacaccaa tcgatgggac taccgacttc aaaatcccta tgtctttttt ccggttgggc
    21601 atgtacattc cattcatcaa acgctagata cattgttttc tcacttctca aatatccttt
    21661 tacataatca caagtagcaa cgatttcttc aatttgacga tcgaatcgat aagcttttgc
    21721 aaggtatgtc gctgtatcat cacgctcatt tttaggaggt ttagtcaagt cttcttcatc
    21781 atacttatca atatagttat gcaaagctaa ataatctaca ttctcataag cttcttctaa
    21841 tgcgatacga tcccattcag ggtacgatgg taaacgtggc gtagagcttc caactaagat
    21901 tgtttcaatt gaatcatcca ctaacttcat gacttttgag gcttcatttg ctaacctacc
    21961 atattcataa gcactcttag cccccacttg ccatggaccg tctagctcat tccctaaaca
    22021 ccataattta acgccaaaag gctttttttg cccatttttt attcttaaat cactccagaa
    22081 ggttcctttt ggaaaattac aatattctac taaatttttt gcttcatcaa ttccttttgt
    22141 tcctaaattc accgtgaata tcggtttcgc ttgtacatga ctgatccatt taaaaaattc
    22201 gtggagtcca aaagaatttg gttctatacc tcgccatgct aaatcaattt tttttggtcg
    22261 ttgttctttc ctacctacag tatccaccca atcataacca gatgtaaagt ttcctccagg
    22321 atagcgaatg acacctaatt ctaattcctt tacataagag aggacatctt gtctaaaccc
    22381 gtcaccatca gctgtttcat ggcccggttc ataaatccct gtatagacga cacttcccat
    22441 atgctcaaca aatgagccaa acagccgaga actgatctct cctaccgtaa aatcctctct
    22501 gatttttact ttcacttctt cgtccaacag ctttccctac ctttcatttc ctatagtcaa
    22561 gataatagat acaagtttcc atacgccacc agcaaagata aaaataaaag gagggaaaag
    22621 aaatatgagc agtacaaatc ctaataacaa tacgcaacaa agaatatttc tcaaaaaatc
    22681 aaaaaatact ttccatgcac tttgttcaaa aatttgtaaa aatgtgatat ctttgttttc
    22741 agaaatcaca tatatccagg ataaaagaaa catggaataa ataagtaaaa ctacatatcc
    22801 actaattgaa agtacgattg tctgttcttc taataattga ttaacaagaa aataaataat
    22861 gatcgttaac agactaagga acaagttctt accaaaatgt tgctttattc cttgaaagaa
    22921 aaaataaatg atatttcctg atccctcacc tttccattgc gccattacat aaaaaacagc
    22981 acatgtagca ggcacgatag taataacagg caaacacacg attacccaga gaatacctaa
    23041 taagatgtaa tcagaaacca caatcaactg tccataaagt ttagtctcaa ctattttcat
    23101 cttcctcact ctttcattcc agacatagct gttccttgaa caaaatattt ctgtgcaaaa
    23161 atataaacca ctagtaatgg cagcattgaa ataaccgttc cagccattac aggtccataa
    23221 aaagaaatgt tctgccctgt aaataaagct aaccctgctg gtaaagttct catttttgaa
    23281 ctacttgtca atatcaatgg ataaagcaag tcattccaac tattcatcag cgtgaaaatc
    23341 cctaatgata aaagagtagg tttgctcaat ggcaacatga ttttcaaaaa aatattaaac
    23401 tcacttaatc catcaatgcg tgctgcctct tccaaagaat ctggtaaagt aataaaaaag
    23461 gaacgcatca tgaagatacc aaatgcagtt gtcatacgag gaatgatcaa acctgcataa
    23521 gtatcaagca tgccaagcaa attgacttca ataaatagag gaatcatgaa gacttggaat
    23581 gggatcatca ttgtcagcaa aacgaaatag aacaaaactg attttccttt gaaacgcata
    23641 cgagcaaatg cataccccgc aagagaatca aagaatacag aagtgatcac tgtccctcca
    23701 gcaaaaatca ctgtattttt gatgtaatct aataaaggga tcgttgtcca aaccttaata
    23761 tagttatcta gggtatagct atctgcaaat attctaggcg gaaatgagat aatgtctttt
    23821 tcgtgtttaa aagaagaaat aatcagccac aaaaaaggaa acgcaataac caacaatata
    23881 actagcatac taatcgttcg aatgattttt ttaccactaa ctgcttttcg actattcaca
    23941 gtaactgtaa tcgagctttc tggtgccata caggtttccc tcctatttca tttgtttttc
    24001 tttccgtgac atgtacacat tagtggagat agctatagtt gcaacaatga taaacaaaac
    24061 cgaagacagt gctgatgcat agcccaattc ataaggtgct gtaaatcctc gactgtaaat
    24121 atattgaaca gctgtttctg tcttaaattg tgggccacca gcggttgcaa catatacttg
    24181 gtcaaacact tgcatcgaac caatcagatt cgttaaaata caaaaaccta aggagggaac
    24241 gatctgaggt agcgtaatgt taaaaaactg ctgtcgattt gttgcaccat ccatttgagc
    24301 agattcatac aaagagggag agattccctg aatagcagtc aagagaatcg tcatagtaac
    24361 accaaaattc ttccaaacag tcatgatggc taccgtcggc atagcaaggg ttggatcgcg
    24421 aaaaaattga gggatcggca agccgacttg tgaaaacaag taaggaatcg cccctacatt
    24481 cggatcaagc aacatagaaa aaataatcgc aattgacgtc aatgaacaaa taactggaat
    24541 gtaaaatgtc gaacgacaaa aacgattgaa tctggtattt ttagataatg ctactgctgc
    24601 taataaacca acgataattt gtgttggcgt ttcgagtacg gtgaaatata aagaatgaat
    24661 caacgaatta actgctcgtt catcactaaa tatacgtgtg aaattttcaa aacttacaaa
    24721 atttcttgat gtaaagaaaa tattcaaatc tgtaaaacta atagcaaatg ttcctaaaag
    24781 cggtatgaca gtaaataata ataaaactaa aaaagatgga gttaaaaaca aatacgcagc
    24841 acgatcagga cgattccaat atttggtgaa tccgttcata tagtccacct catctttcat
    24901 tttgataaaa tatacaatct tggtttaaac gaatctgaat aagatatccg aacaatcgct
    24961 gtgatttttg caataacacc tatctgcaaa tactaggtcg atcattcaga tatctgtatc
    25021 ttgccaacag tctatcctgt ataaagttct gtactatttt tgcagtaatt gattgatggc
    25081 atcatctgca tcgttcatga gtgtctcagg atcttctcct gctaataatt tttcaatcag
    25141 tggattgatg atatcgttat tgatcgcaga gatttgggta aaacctggta aatatggctc
    25201 tgcatagtcc atttgtttcg ataattcaga tacaatagga tcatttttta cttcttcatc
    25261 ttttgctact gaatccaagt aggcaggaaa accattttcg atactccatt ttttaccaat
    25321 ttcagttgta ttccaatatt tcaaaaactc atagacagct tcttttttat tattatctgt
    25381 acttgccgga atggcatatc ctacgccatc taaaatagct gtttttttgt ctgaatcact
    25441 cccttgtggt aaggtagtca cattataatc aatctcgttt gctttcaatc cgttatttaa
    25501 ccatggccca ttgatttcaa tggctagttg accagcattc attaaattgt ccatttcggc
    25561 gccagaaatc gattctggac cagtcttgtc tacatggatc attttttgga tcgcttttaa
    25621 cgttttaagg ttctcaggag acgcaaattc agacttagta taatcatcgt tcactaattt
    25681 accgccattt gctaacatcc agttatataa cggtgcagtt ccatccttat tgaagacaaa
    25741 acctgataca tttttactac tatcagttaa ttttggtgcc atttctgtca attcttccca
    25801 agtagtaggt ggttgttcag tatctaatcc agcttgttta aacaaatcat tattccaata
    25861 catatacatg ccttgtacct gcatggggac aaaatactgt ttaccttcta cttcacctaa
    25921 ttcaatagcc gtttctttaa aatcagattt atctactcca tcatattccc agaaatcgtc
    25981 taatggttgt accgctccgt tttctacata ctgtgcaaaa tctggataat ttaatgcaat
    26041 aaagtcaggt gcttttttag aagaaatcgc tgtaggaagt ttttcattca aattggccca
    26101 agtcatgata tccatttcaa ttttgatgtt tttatcattt gttttattaa attcattaac
    26161 gatttcttgc aatatttcac catcagatgc agtaaaccca ttccaaaaag tcaaagtcac
    26221 ttctttatca gaatcagatt ctgctgaact tccgccaccg caagctccta aaatacctgt
    26281 tgcagctacc aacaaagagc caattaacca ttttttacct ttcatttttt gttcctcctt
    26341 catctgataa acgacatctg accacattcc aagatagcgg tttcaataaa caattgattt
    26401 tcccatcttc agcttttaca tttgttgaat gacttggttt tacttgttca cttttaggaa
    26461 gattagttgc ttttgtatca aatccactca tttcagaaaa atcaatcact ccctctaatg
    26521 ataattcaga caagtgaaca gttagattca tttcttcttc tccacggtta actgcaaata
    26581 tgacaagttc tttgtcattt attacggcta tactttctaa atacggcact tcttcaaaat
    26641 cttccgtcgc atagtttgct gaacgaattt gtgggcttaa cacctctcct tgtccataca
    26701 cagcagcttg cataaatgga taatagatcg tctgtctcca agcttctccg tttgtttctg
    26761 tcctgattgg tgcaataaca tttaccaatt gagcaagaca agcaatcttt actctatctg
    26821 catgtttcaa taaagtgatt aatagacagc ctaatagtaa tgcatcttca aaattatagt
    26881 tatcttctag taaaggagga gcaatctgcc aaggcgctgc ttgctcatct tgttccgctg
    26941 aatgatacca aatattccat tcatcaaagg acaggttgat ctgcttctta ctatgtttct
    27001 tcgccttgac tgtatcgcac atcgctgtca cttcagaaat gaaccgatcc atgtctaagg
    27061 aacgtgcaag ataatgagaa agatcatttt tttggttgcc ataatattga tgcagagaca
    27121 aataatctat ttgatcgtac gcttgatcaa gaactgttaa ctcccaatca ccaaatgtag
    27181 gcatttgccg attcgaactt ccgcataaaa ccacctcaat tgaatcatct acgagtttca
    27241 tcgccttagc cgtttcctct gctaaccgtc catattcttc cgctgtttta tgtccaatct
    27301 gccaaggacc gtccatttca ttccctaaac accatgtctt aattccaaaa gggctctctt
    27361 gaccattcga acgccttaaa tcactccaat aagttccttt aggaaaatta caatattcga
    27421 ctaaattacg agctgcatct actccacgag tacccagatt gacagccata ttgatttcgg
    27481 cccccacatg ctgacaccag ttataaaatt catgaatccc aacttcattc gtttcaattg
    27541 tccgccaagc taaatccaac cttctaggtc tatttgctat gggcccaata ctgtcttccc
    27601 aacgaaatcc tgacacaaaa tttccaccag gatatctaat catgggaatg ttcaattctt
    27661 ttaccaagtg aatgacgtct tggcgaaatc cacttttatt agctgatgga tgatctggct
    27721 gataaatccc ttcatatact gctcgtccca tatgttcaat aaatgaactg tacaaccgcg
    27781 gatcaacttt gcctacacgc ttgacactac taatcaacat tgtggcttcc ataactgacc
    27841 tcctttaatt tatacatgaa taatacacct cgttgaaagc gttgtcaata atataaatgt
    27901 taaacttact tattttttat cgattttaat taaccaaaat attaataatg attaaaacaa
    27961 ataatttaat ataactttta ttaacaaata tgttaatata taagaaaaac cttttaagag
    28021 gtgatatata tgcaactaca aataaacgaa gaatccttac cggtatatga agcattagct
    28081 agtaaaacaa ggatcaagat catccagcta ctttccaaaa aaaagatgaa tgtcaaagat
    28141 ttggcaaaag aacttggagt gagcagtgct atcacgacga tgcacgtgaa aaagctagag
    28201 gaagcaaaca tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag ccttcgtgtc
    28261 gataaaatcg atatcagttt tccagaaaaa atattcaatg cttttgatac gaaagaaaca
    28321 tcgataccta ttggtcacta tacaaattat gcaattgaac caacatgcgg tttagctacg
    28381 atccatgatt ttatcgggaa agtggatgaa ccacgctatt ttatggatcc tcgaaggatg
    28441 gatgcccgca ttctctggtt taccagcgga tttgtcgagt atcaggcacc taatttttta
    28501 aatacagagg atacattgga gatgttggaa gtttctgtgg aaatttcttc tgaatttcct
    28561 ttttcaaatg acaattggcc ttcagatatc actttcagcc taaatggtgt ggagctagga
    28621 acatggacga gccctggaga ttttgctgat atccgcggta aatatacgcc tgattggtat
    28681 ccagataatc ttaatcaata cggcttgctg aagacgatcc gaattaccaa acacttgacg
    28741 aatatgaatg gagaaccctt gtcaaacatc acgatcaacg acatccctaa agagcaagac
    28801 acttggcatt tgcgaatcga agtgaaagac gatgccaaac atgtgggggg ctgtacttta
    28861 tttggtaaag gattcggtaa ttacgatcag gatatcaaat taaaagttta ctacagctga
    28921 tatttttctt ttctatttta taaaacaaaa acagccaatc cctatgaagg gacggctgtt
    28981 tttgctttat tgttatttcc agaaatcatc caaaacggaa attggcaaat gtcgtttatg
    29041 ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa cttcttttcc
    29101 ttctagataa tcatcaattt cattatatgt gacacctagt gcgacttcat ctgcaatcat
    29161 tggtttccca tcttcaagat ctgcagtcgg aatttttgtg tataattttt ccggtgcatt
    29221 caatgcttga agcaattgtt tcccttgtcg tttgtccaaa cggaataatg gcaaaatgtc
    29281 ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag catgatctgt
    29341 ccctaatacg gctcctgctc tttctccagc tacggcatat tgcgtaatca tacgttgacg
    29401 tgctttgata tttcctttgt taaaatcgct gattcccaca cctgcttcgc ttaatacttg
    29461 tacttgtgca tcaacagctg gtttgatatt tactcgcaga ctaacatcgg gctggataaa
    29521 gtcaagagca gcttttgcat cttcttcatc tgcttgttca ccataaggaa gacgaacagc
    29581 gataaattga tagttttcat cttttgtttc ttcacgcatt tcttccatcg ttagttgcgc
    29641 caaacggcct gctaaagtag aatcttggcc gccgctgata cctagtacaa aagttttaag
    29701 aaacggatat tttttcaaat atgctttcat aaagtcaata cttttacgta tttcttcttt
    29761 tggatcaatt gttggtttga cacctaattc attgataatt tctgtctgca ggctcattta
    29821 ttctcctcct atgcttctgt atgaagttct gctacgcttt ttttcattcg ttccatgata
    29881 gccattttat ggttccagca ttcagtagac agatcgactg ggtatttttg cggattcaag
    29941 tctcgtttat actcttccca tagtgaatct agattttctt ttgtatattc tttgatttcg
    30001 tttagtgcag gtaattcgta aactcttttt ccttcgataa agatatcttg taaaactggg
    30061 cgagctgtaa agtctcgaac agttttattg ataaacgtat gcactggatg gaacatgaag
    30121 atagcttctt cttctcgagg gtcttcatcc cataatgtca cgtagtctcc ttcagatttc
    30181 ccatcaaaat ttcgagtgat gcgccacact tgttttttcc ctggagtagt tactttttct
    30241 gcattgcttg ataacttgat cgtatccatc atttttcctt catcatcttc gatcgatact
    30301 agcttgaata ctgcaccaag ggctggctga tcataggctg tgatcaattt tgtcccaaca
    30361 ccccatacat caattttagc tttttgcatc tttaaattca aaatcgttgc ttcatccaag
    30421 tcattcgaag catagatttt tgcttccgta aatccggctg catctaattg ttcccgaaca
    30481 cgcttagata tataggccat atctccactg tcgatgcgta cgccttggaa gttgattttg
    30541 tcacctaatt ctttcgccac gcgaatcgca cttggcacac ctgatttcaa cgtatcataa
    30601 gtgtcgacta agaacacgca gtctttatgg gttttagcgt aggccataaa tgcttcataa
    30661 tcatttccat aagattggac taacgaatga gcatgggtcc cgctagctgg tatcccaaat
    30721 atcttcccag cacgtacatt gcttgttgca tctgctccgc cgatatatgc cgctctagtt
    30781 ccccagactg ctgcatccag ttcttgtgcc cgtctagttc caaattccaa tagtggatct
    30841 tctccgatga cagatttgat acgagctgct tttgtcgcga tcaacgtttg gaaattgacc
    30901 atattcaaca gtgctgtttc tactaattgg cagtgcgcta aaggcccttc cacttggatt
    30961 aatggttcat ttgcaaaaac tagttctcct tcacgagcag aacgaactgt tgctttaaat
    31021 tcaaaatttt gtaaatactc caaaaaacct tcaggataga cttctaatcc acgcaagtaa
    31081 gcaatatcgg aatcattgaa cgtcagattc tctaaataat tgactaaccg ttccaatcct
    31141 gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa agtaacattc aaaaacagca
    31201 tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca tattgatttg atatagatcc
    31261 gtatgcagcg tcaaactgtc gtctgggtaa gtattattca tcttgtatta ctcctcttgg
    31321 caagttttca tttcctaccc attataacaa aaaaaattaa taggaaaaga aatcttccat
    31381 tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac tccatctgct
    31441 a
//
LOCUS       DAGCYL010000014        30073 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.014, whole genome shotgun sequence.
ACCESSION   DAGCYL010000014 DAGCYL010000000
VERSION     DAGCYL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.014"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..435
                     /locus_tag="MB216_000471"
     CDS             <1..435
                     /locus_tag="MB216_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBT4525331.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="MB216_000472"
     tRNA            complement(759..842)
                     /locus_tag="MB216_000472"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="MB216_000473"
     CDS             1370..1870
                     /locus_tag="MB216_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4525332.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="MB216_000474"
     CDS             complement(2060..2404)
                     /locus_tag="MB216_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBT4525333.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="MB216_000475"
     CDS             complement(2558..3478)
                     /locus_tag="MB216_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4525334.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="MB216_000476"
     CDS             3665..4156
                     /locus_tag="MB216_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBT4525335.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="MB216_000477"
     CDS             complement(4180..6555)
                     /locus_tag="MB216_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBT4525336.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="MB216_000478"
     CDS             6881..7507
                     /locus_tag="MB216_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBT4525337.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="MB216_000479"
     CDS             7530..8234
                     /locus_tag="MB216_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBT4525338.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="MB216_000480"
     CDS             8426..9259
                     /locus_tag="MB216_000480"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBT4525339.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9456..11384
                     /locus_tag="MB216_000481"
     CDS             9456..11384
                     /locus_tag="MB216_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBT4525340.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFSESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11522..11977
                     /locus_tag="MB216_000482"
     CDS             11522..11977
                     /locus_tag="MB216_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBT4525341.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12447..12716
                     /locus_tag="MB216_000483"
     CDS             12447..12716
                     /locus_tag="MB216_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525342.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            13116..13370
                     /locus_tag="MB216_000484"
     CDS             13116..13370
                     /locus_tag="MB216_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBT4525343.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(13459..14505)
                     /gene="holA"
                     /locus_tag="MB216_000485"
     CDS             complement(13459..14505)
                     /gene="holA"
                     /locus_tag="MB216_000485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBT4525344.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(14543..16816)
                     /locus_tag="MB216_000486"
     CDS             complement(14543..16816)
                     /locus_tag="MB216_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBT4525345.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(16809..17348)
                     /locus_tag="MB216_000487"
     CDS             complement(16809..17348)
                     /locus_tag="MB216_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBT4525346.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(17484..17702)
                     /locus_tag="MB216_000488"
     CDS             complement(17484..17702)
                     /locus_tag="MB216_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBT4525347.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(17699..17944)
                     /locus_tag="MB216_000489"
     CDS             complement(17699..17944)
                     /locus_tag="MB216_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBT4525348.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(18060..18788)
                     /locus_tag="MB216_000490"
     CDS             complement(18060..18788)
                     /locus_tag="MB216_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBT4525349.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            complement(18947..19990)
                     /locus_tag="MB216_000491"
     CDS             complement(18947..19990)
                     /locus_tag="MB216_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBT4525350.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(19980..20471)
                     /gene="coaD"
                     /locus_tag="MB216_000492"
     CDS             complement(19980..20471)
                     /gene="coaD"
                     /locus_tag="MB216_000492"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBT4525351.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKNL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(20468..21028)
                     /gene="rsmD"
                     /locus_tag="MB216_000493"
     CDS             complement(20468..21028)
                     /gene="rsmD"
                     /locus_tag="MB216_000493"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBT4525352.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(21147..21485)
                     /locus_tag="MB216_000494"
     CDS             complement(21147..21485)
                     /locus_tag="MB216_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBT4525353.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(21469..21906)
                     /locus_tag="MB216_000495"
     CDS             complement(21469..21906)
                     /locus_tag="MB216_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBT4525354.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(21896..23020)
                     /locus_tag="MB216_000496"
     CDS             complement(21896..23020)
                     /locus_tag="MB216_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525355.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(23041..26469)
                     /locus_tag="MB216_000497"
     CDS             complement(23041..26469)
                     /locus_tag="MB216_000497"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBT4525356.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(26495..27658)
                     /locus_tag="MB216_000498"
     CDS             complement(26495..27658)
                     /locus_tag="MB216_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBT4525357.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(27661..27963)
                     /locus_tag="MB216_000499"
     CDS             complement(27661..27963)
                     /locus_tag="MB216_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBT4525358.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(28077..29849)
                     /gene="recQ"
                     /locus_tag="MB216_000500"
     CDS             complement(28077..29849)
                     /gene="recQ"
                     /locus_tag="MB216_000500"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBT4525359.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
BASE COUNT         8856 a         6100 c         5229 g         9888 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct tatttgagaa atgttctggt
     9301 tttttatttt ctttaataat aatcattata aataataaaa acaacatcaa gcttaatcac
     9361 caaatgataa ccattatcgt tctcatttag ctgttattat tatcaattac atttttatta
     9421 tgctatcctt gttttgacaa ggaggtctaa taacgatgac taatcagaaa aaagcttttg
     9481 cagcaacaat cggctgcctt ttatttatga tcttcggttt tatcttcgac cgattctctc
     9541 ttaccatcac cccacttttt tatatcagtg ccattatcat cggtggatat aaacaaacat
     9601 cggaaggctt gaaagaattg tggaatgatc ggacactgaa tgtggattta ttgatggctt
     9661 tagctgctat tggtgcctgt ttgatcggta attactttga aggagcaatg ttgacattca
     9721 tcttttgttt aagcggtgca ttagaggaat acacaactaa taaaagtcaa aaagaaatta
     9781 ctgctttgat gaatcttcaa ccacaaaaag cccaattatt aaaagaaaat ggacaaatgc
     9841 aagaagtaga tgtttctcaa ctaaaggtgg ataacttggt atttgtcgct aaaggagcag
     9901 ctgttccgat agatggcttt tcagaaagca cacaggcaac gatcgatgag tctgcattaa
     9961 gcggtgaatc tgtgcctgta gaaaaatatt taggcgatga gatgtatgca ggaacgctga
    10021 atcaaggaaa tcccatcacg attcgagtaa caaaaacgag ttccgaaact gtctttgcta
    10081 agattatcca attagtagaa gaagcgcaga atacgccaac tcaaacagct tcatttatcg
    10141 aacgaatcga aaataattat gtaaaattga ttttgcttgc tgttcctcta atgatccttc
    10201 tcccccactt tttcctaggt tggagttggg atgaaagctt ttatcgagga atggtattat
    10261 tagttgtggc ttctccttgt gcgcttgtcg catctgctac accagcttct ctagctgctc
    10321 tttcaaattc tgcaaaaaat gggattttaa tcaaaggcgg tatccattta gaaaagcttt
    10381 ctgaattaaa agcgattgcc tttgataaaa caggaacgct cactaaagga aaaccagttg
    10441 taactgacag ctttttcttt gatgaaaagg atttggcaca acaacttctt gttgctatgg
    10501 aacaaaaaac gacgcatcca cttgctcatg cgattttgca atatttcgat tgcacaatac
    10561 ctgaagaaat ccaatactta ccaatagaag aaattactgg ttttggatta caaactacct
    10621 atttaggtgc gcaatggaaa gttggcaagc acgcatatga tcctgagacg atgataatca
    10681 gcaaagagat tgcggaaatg atcgaacgtt tggaaaatca aggcaaaacc gtgatttatc
    10741 tttctaaaga tcagcagtta attgctgttt taggtctgct agatattcca aaagcaaata
    10801 cccaacaagt aatttcctac tttaaatccc aaaacatcca tacaagtatg ataaccggcg
    10861 accattctgg cacagcaaag gccattgctg aacaagtagg gattgaacat tactatgcaa
    10921 gctgtactcc ggaagaaaaa acacaactag tcaaaaaaga gaatgaactt taccaagtaa
    10981 atgcgatggt tggtgatgga gtcaatgatg cgcctgcttt agcagccgct tctttaggtg
    11041 tcgctatggg gcaagggaca gatgccgcta tggatatcgc agacatcgtc ttaatgaaaa
    11101 gcgacttaaa taaatttgtc tacagtcatc ggctatcttt aaaaatgcgg cggatcatca
    11161 aacaaaatat tattttttcg attcttgtta ttctcttgtt gattgcttct aacttcctgc
    11221 aatttttaaa tctacctctt ggagttgtag gacacgaagg aagcacaata ttggttattc
    11281 tgaatggctt gcgtttacta agacctttac ctgatctttc tgattcttca aaaaaatgta
    11341 agtcttgtcc actatacaaa gcgcggctgt cttctgtcaa ataagcaaag tgttttttta
    11401 tgattttgtt tattttatga aaaaaacagc ttatgaatag gtcttgaagg atgaattcct
    11461 acttttgtaa ctttctaaaa acatgttatg attgtttcga agaaagtgag gaatatattt
    11521 catggaaagt tggctttttt taggaattat tttacttgta gcaattttag ctaaaaatca
    11581 aagtctgatg atagcggcag cttttgtttt gattatgaag ctcataccag cctcccgttc
    11641 ctttttccct tggcttcaag gcaaaggaat caatattggg gtgacgatca tcacagcggc
    11701 tattctaatc cctatcgcaa caggagaaat cggtctacgt gatctattag aagcctttaa
    11761 atcccctatt ggctgggtag ctgttttttg cggtgggcta gtggctgtct tatcttccaa
    11821 gggtgtagga ttgattgctt ctgatccaca aatcactgtt gcacttgttt tcggcacgat
    11881 tttaggtgtt gtcttcttaa gaggaatcgc cgccggaccg gtcattgctt ctggtatcac
    11941 ttactgtatc ttacaagtat ttgctttagg aaaatagaac aaattttgtc tattttagga
    12001 gattcttaac acccccaatt tactgatccg tgatatactg acaacgaggt gatacaatgt
    12061 tcgaaaaatt aaagtatttc ataggaacaa tcggctggtt tcttctagcg gtacttttga
    12121 ttctgggctt agcttatcta ttatttcctg atttgataca aaatgtgttt gatactgctt
    12181 tttgattgac ctattaaaac gatatagggg atgctcctgc gatgcagggg ctttttttaa
    12241 tcaatgtttt gtaataattt aggccgtgcc aatgagactt cctatagaat tcgatgctct
    12301 tttttctttt tggctcttct gctctttttt gttatactag attacagtgg acaaaagaaa
    12361 gaatgatcgc atccacttca acgaatatca aaatggaaga attagtgcct gttttcaggt
    12421 tactttgaaa agaaaggaag acgcttgtgg aaacttttga aggtctttta attgatacgt
    12481 acgttttaca agaacagcca atgatgctga ctgggatcaa cttgcagctt gaggaccgtg
    12541 tgatcaatgg cctgcttaaa gatcgctatt tggcaaaata tatcttacgt ttgcccctaa
    12601 ataaatatgt gctggaagta agtggcaaat ggaaccgtcg gaaccagttg gtcataaaaa
    12661 aaatgttgat aaaaaatatc gatgaataca ttaaaataat cggtacacca gaatagcctt
    12721 tactgatcaa aggatactgg ttataaaata actgcagttt cttaatagaa aaacaaagaa
    12781 caacagggaa tcgtatccct tttcaaaaaa aactttgcaa acggttctct ttgttatata
    12841 ataacttagt accaaacatt cccattcaaa ctttgtttaa atagataaat aaaaatctag
    12901 tttggtttga cgctattgcc tcccacaata gcgtcttttt gatttttttc agcttgtact
    12961 atccgttttt actgaaaaaa aatcctaata aaagactgtt aaggaaaaaa acttgtcatg
    13021 ttcatattgc aaagcacgct gcctttaggt ataataatga atgttgaatt atgcggtaac
    13081 acgcgtaaga acttaagtta tcggaggtga aactaatgcc aaacattgaa tctgcaatca
    13141 aacgtgttcg tacaagcgaa aacgctaatg tgaaaaattc atctcaaaca agcgctatgc
    13201 gtactgcaat caaaaaattt gaagatgctg tagcttcagg tgctgacaac gtagatgcgt
    13261 tatataaaga agctgttaaa gctattgata tggctgaatc aaaaggtcta atccacaaaa
    13321 acaaagctaa ccgcgacaaa tctcgtttaa gcaaaaaaat cgctaaataa aagaatcggc
    13381 ttcggccgat ttttttattt gtcaaaaaaa ggacaaaaac aaacgacctt cccgcatgct
    13441 ttgccctcgt ctttcttctt atcgatttct taacaactgc cctgtgcgca aaacgaatag
    13501 ttgaaaactt aattcttttt cgatttgtcc cgttttgatt tgataatcca tctcaactaa
    13561 ttgatcaaac aactgtacca gcaaatgttc atcgaatttt tttacttcct gcatggcaag
    13621 ttttacacga tatggatgga ttttcaatgt ttcagcaata tttgcctgct gataaccaat
    13681 ctttacaaga aattttgtct gtaaaaataa acgtaattga gaaagcaaaa tcgcattaat
    13741 tttgattgtt tcttcccctt gcataactaa atcttcgtat aaacgcaaag cttgttccgt
    13801 cttccctttt aaaatatatt gggtcatgtc aaaaatatta tgttctaacg ttttaggaac
    13861 taattcttct acttctctta ccgtgatttg ctgttgattt tgtccataaa ggaccaattt
    13921 ctgtaactct ctcatcattt tggataaatc taagtcggtc aacctcaaga ataaatcaat
    13981 tgcacttcgt tccattttga ttgaggaatt ctctaaggta ttcatcagat attgacgtac
    14041 ttctttttca ttcaactgtt ggacattgat gataattgct gtttttttca attgtttcgt
    14101 tacttttttc cgttcatcca acttttcata tggcgcaaag aatactaata cagtcgattc
    14161 taatggttct ttcaaataag ccgtcaataa atcagtattt tgttcgattc cattgtttac
    14221 tttttcacct gttaaaaaat aaggattctc tacgaatact aaccgttgat ctccgaaaaa
    14281 cggcaacgtc tctgcttcag ctactacctc atctaaactt gcattttcca tgtcaaaact
    14341 taaaaaattt aagtcatctt cgccctctaa ttggattttt ttgacaattt ctgtccgaac
    14401 ttgatcttgg agaaactttt ctgttcctaa aatcaagtaa caagatgcaa gtttttcttt
    14461 acgtattttt tgtaactctg cttgtatact caaattactc accactttca aaaattatcc
    14521 taccatgagc agatgagaat tactagtcta tcattgtttc cactagccca tgaggatgaa
    14581 aaactggatg ccagcgatag gtgatcattc cttgttgatc cgttcgccat gtttttatct
    14641 gattttcttc tagtgtctcc aatacttcgc tgtgcgggtg accataacga ttatttacgc
    14701 cgcaggatat aatcccatgt tttgcttcaa tatgggaaag aaaatctgtg ttacttgacg
    14761 tacggctacc gtgatgacct aatttgacta catcggcttt cagattagga taaatcaaaa
    14821 gcaattgttt ttccattgct tgattcaaat ctcctaaaaa taaaaagcgc ctattgttat
    14881 attccatcca tagaccaatc gaatcttcat ttcttccctc tcccttttca aaaggagtca
    14941 atacataaag atcataataa ccgctgatcc tgtccccatg tttgatttca atgatctcag
    15001 taccattttt ttgagcttca gataaatgcc tttgtaatgg ctcatttcgt ccagcgcccc
    15061 aaccgatata aatttttttg atagtaaaat gctgagataa cgtgggtaat tctcccacat
    15121 gatccgtatc gtcgtgtgtc aagatgagtt tatcgattac acttatcccc tgacctttta
    15181 gatatggaac gatatttctt tcagcaggag gacgtatcat accttgcgcc cacttttctc
    15241 tttcaaagtg taactgacct ccagtatcga tcaaaatcgt ttcttttcga aaaggacttt
    15301 gcaaaaagat actgtctcct tgaccgacat cgataaaagc gatcgttgaa gaaaagcgta
    15361 aaaatggcaa agtcaaaaaa ataaaagaaa aaagaccaac ataaaacaag tgtttcacag
    15421 gttgttcttt caacctatcc atacaaatca gtaatccgaa aatatacatc ccagatagca
    15481 caaggggcaa acgtcctata atgagagacg accctgaaga aaaatcgatt attcgctcaa
    15541 ataagattaa tccctcttct atcagcaaaa gtaaagtttc cggcagccag cgattgaaga
    15601 gtgtgagaaa taacacacca ggaagaaaaa gaaacttgaa taccggtgca atcagtaatg
    15661 taaaaagact acctaaaata ggccactcat aaaaatgcca tacaatcaac ggcatcgtga
    15721 tcatagtaaa caggacgtga gaggccgttt ttttcaacca atctccatca aaagaaatca
    15781 ggataaacaa aaaactcatg aaaaaactaa gctgtccacc cgcatgaaaa agaaatcgag
    15841 gatcaaatag taaaagaagc caaaagacaa ctgcaaataa gtccatggtg gaataatgct
    15901 tattcagcaa tttgactagc aatcgcaata aaaacagcaa actagcacgc aaaacagaaa
    15961 tagaacttcc tgcaatagaa acataacaga tagttagtag actcaacgga ataatactga
    16021 cgtgtagtgg ccatccgata cgtcgaaata aataatacag ccaccctaaa taaaattgga
    16081 tgtgtagacc cgatagactg aacagatgca gcaaaccact ctccctaaaa atttcttctg
    16141 gttctctgaa tcctgcatct ttatagcccc ataataaaga attaatatat acacttgttt
    16201 tttgcggaaa ttcagattgg acaaaggaga taaaagatcc tctttttctc tgaagccagt
    16261 ttcgccagtg attttgcatg gtgatctgtt caatcttttg aatttggaac cgtccagaaa
    16321 ttctatggtt cctgaagtaa gcttcttggt caaaattata aagattccgc ttttttgctg
    16381 gagagatgaa ctcgccttca gcagtcaatt ctattccctt tcctcggaag ttaagccatg
    16441 ttttcaattc tgcttctgtg tttacttgat aagaaagctg gagcttttgt ttattctctg
    16501 ataaccttcc ttccattgtc aaaaaattac catttgcacg aatcgtatct gtaaatacag
    16561 taatttgcgc agatgttgtt ttttcctcta aaaatgaata ctcttgtagc ctataagaaa
    16621 tggataccgt tatgctacac aaaatacaca agtacgcgat aaaacgtcct tcttttagcg
    16681 aaaaccataa aaaaattccg gcaagaagca aacgataacc ccaattgtca gtgtaataca
    16741 acaaacagat aatcatcgac gtaattgccg gaaaaatcaa ccgatttcga acatgccttt
    16801 ctaaaacgct agacatgttt tcctgttaat tcattttgaa gttttgaaaa atgctgtgag
    16861 tctaaagcaa cttggtgtac agatacacct aattgttcga tcaacgcttg ggcataagga
    16921 tcatttcgat agtcatggag ataattgatc cctttcacac cagcttgcaa caaagccttt
    16981 gtacaggcca aacatggaaa atgtgtgaca tagatttctg aaccttccgt cgggatacca
    17041 agttttgcac actgtaggag tgcattcatc tctgcatgga tcgttcgcaa gcagtgtcca
    17101 tccacgacgt aacacccttc atctatgcag tggacatctc cactgactgc tccattatag
    17161 ccgccagcaa tgatcctttt ttctcgaacg agtgtcgctc ctacttctaa acgtgtacac
    17221 gtactacgta aagataacag tactgcctga gccataaagt actggtccca aggaatgcgt
    17281 tcattatgtg acttatcagt aactttttct ttattcgtat cgcatgcttt tgaagatttt
    17341 tctaacatgt tttatattcc tccgtttttt caagttgatt aaaaagtact cattgcttaa
    17401 acaaaatatt ctttaaatat aacgaatcat cctgtctgac aaaagatcct acccttcact
    17461 gccgaaatga agtccgtcat ccattagccc attataaaaa ttcttcgaat ccatttgttc
    17521 aagaaattcc gtataaaaat cgatacttca caatcatggc gttttgctac ttctgtgaga
    17581 acagagatat attctttgat caacttgtta ttccgatatt tttgttttgt ctcatcgact
    17641 gctggtggcg tgataaagaa agcatgatca gaactatagt cttttttcag ctgaactatc
    17701 atccactgca gattttttgg tacgtttctt tagaaagttg tttatgctct gctagatcat
    17761 tcgtcccaat gagaataaat actttatctg ctgtatagct aagtacatct ttggcaaagc
    17821 gttctcgtag agcttgactg ttattgccgc tgacagccgt attcataata tgaatcccag
    17881 atattttatt ttggagatag acattcagca ttggtttttt taatgtttct ttgcgaacaa
    17941 tcaagctatt tcctatccaa aaatactacc tcctcgtcct gccaagctct tagaattgga
    18001 ttattcttag tatccattag cgtccattta ttgctcgtgc cagtcgattt tgaatcattc
    18061 tatttaattg taacgaagtt ttttagtttt tcaactgtct tttcaccgat accagaaatc
    18121 tcctgcaggt cttccacttt ctggaaggat ccgttttctt ctctaaatcg aatgatttct
    18181 tgcgctttga ctgatccgat cccaggaatc tgctgaagtt ctgatggagt ggctgtattg
    18241 atatttattt tgggaggctc ggaatctgct gtaggctgtt ttccttccga ggatgccaac
    18301 tgatcaagat gttctggcag ctcttcacct acagcaggga tatataggac ttgctgatct
    18361 tctatcttcg cagaaaagtt gattcgatcg ctatccgctt cttctaataa tccacctgca
    18421 cgctttagta catcatagac ccgttcttct gaagaaaagg aatagatacc aggctccttc
    18481 acacttcctt tgatatcggt gtacatgacc tttggacgag catcctcttc tacatcgttt
    18541 ccttcagctg atggatcttc tccactggaa ctaaacgaag aaagtatttc ttttgcagca
    18601 gtctcttctg tttctctata aaatagcaaa tttgataata aaaaaccact tgcgatacaa
    18661 aagataagaa ctatcataga catccaaatg atcaatttgg gtgaagctag tattttcttg
    18721 ctgatttttt ctatcattgt tcttttttca aagaatgacc agtaaaaatc attcattact
    18781 cctttcattt aaaattgaag aaacagtgag ctctggcaaa atcgtccctt caacatctaa
    18841 ttacgtcaat tttttctttt gtttttattt ttatcgtgaa gatttttgac gaaaaagagg
    18901 ggttgtgaca gaactcgtgt cacaacccct ctttaattat ttagtttcat ttcattttct
    18961 ctagatagtc aagcgcttct tgtactgttt ttactggtac gattttcatt gaggaattca
    19021 attttttagc ggcagctttt gcttcttcgt agttggtttt tatttcagga tattctttct
    19081 taacatcgtc tgaaatcgta tcatctggtg caaagaaaat ctctacgcca gctttatccg
    19141 cacttgccac ttttttatcg attcctccga ttcggccaac ttctccttca ctgttgatcg
    19201 ttccagttcc tgcaatttgc ttaccatgtc gcaagttttt accagtgact tgttcataga
    19261 tttctaaagt gaacatcaat cccgctgatg gcccaccgat agagccagaa ttaatacgaa
    19321 catctgtatc tgattcgatc tcggtatggt cagtaagcgt gatacctatt cctgcctttt
    19381 tatctgttgg taactcgatc agatcaccag aagctgtttt ttcttcacca ttatgcaaat
    19441 aactgatttc gattgtttgt ccaacttttt ggctttttac ataatccata aatgcttctg
    19501 tactttgaaa gcttttcccg tctacttttg ttactgtatc tccaactgaa agcttgccaa
    19561 agaaattcga tgttttctct acactcatga catatacgcc tttgaattcc attttataag
    19621 gcttgtctgc aagttttaac gcttgttcga ttgctgcatt ttgggaagaa tccatataat
    19681 actgttgcat ttgattatat tcttcatttg tcgccccacc agtcaattct tcttcactga
    19741 tcaattcttc aaaaggggtc attttagctt ttaaagctaa gaaacctgta gcttgcctga
    19801 taccaacaga ggtaagagag aaagacccag aacctttatc ttccttatca ttgaccgtaa
    19861 ttagttcttt cagatcgatc gtggctcccg gtgattctac ataatacggc aagggaacta
    19921 ctacaaatcc aatgatcaac attccaaata tgataatcag tactgttttc aaccattgtt
    19981 tagctttcat ttgattcacg ctttccagct aaagcttcgt tgatcactgg cggtaagtag
    20041 ctgctcacat ctccgccaaa gtgcagtact tcttttaaaa tactggaact cacgtgacta
    20101 tattctggtt ttgctaataa gaagacagtt tccaattcat catatagatg atgattcatc
    20161 tgagcaatct ctcgttcata ttcaaagtct ttgatacttc gtatgcctct gatcaacgca
    20221 tctgcaccaa tctcttttgc tgtttctacc gtcaattgat tttcttgatg catgactttt
    20281 acattaggaa gatgagaaac agcttttgtg atcaatgtca ttctttcttc tgggggaaac
    20341 aaatttttct ttgaagtatt gataaaaaca ccaattacta cctcatcgaa taatttagcc
    20401 gcacgttcca ccgtatctaa gtgacctttt gtaaaaggat caaaacttcc tgggaataat
    20461 gcacgtttca ttgatttccc tcttttctat agatagttac ggccgttatg ccataaatct
    20521 gttttcttat ttgatggaat ccagccacag tttctggcaa ttcgactgtt ttatccgttt
    20581 cgcatacgat aactgcggtt ttagaaaaaa gtcccagttc ttccatttta tggatttgat
    20641 cgattatttt ttgttttgca tatggcggat caagaaacaa gtagtcgaat tgttgtttct
    20701 ccgctgctag ccgttcgatt gctttgtccg catcaagttt tattgtggta aatttctctg
    20761 gttctttcgt aatttcgatg ttttctttga tgatttttag cgcttggtaa tttttgtcta
    20821 cgcataccgc atgactgcag cctctagata ctgcttcgat agctaaacca ccgcttcctg
    20881 caaacagatc caatactgtc tctccatcaa aatatggtcc aatcatattg aatatggatt
    20941 cttttacttt atctgtagtt ggtctggtgt tcgcccctgc taaacttttt aaacggcgcc
    21001 cgccataatt tcctgatatt atccgcatct actcactcct tagaaatggt actggtacta
    21061 gtataccaaa aaaatacttg gacaatgatc aaaaatcgat cgttatccaa gttatttttt
    21121 atttaaaaac tgctcataag agatttttaa agatgttcag ctaatcggat ttctgtattt
    21181 agctcttcaa tttcgaaacc ttcgtctttt aatactgttt tgtttcctat tttttcggca
    21241 aaattcattt cgatatccgg tcggtaagaa cgttctacgt ttcgaacaaa atgtaattta
    21301 cggattcgtt cttctgtttc ttccaatgtc tcttcattta agtaaagcac gacatatttc
    21361 attcttcttg agacataatg caacaagccg aactttttca gattctttaa ttgttttaag
    21421 ctgtagaccc atacgatcag cccgcgtcgt tttgttattt caaattcact attgtctaat
    21481 tgcatgacaa ttacctccgc atccaaagtg ttttttcgtt tcaaaaaacg ggctacctgc
    21541 atctactttg atttcattag aaacttcttc ggcaagtact tggacaattt gatccaacag
    21601 actttgcagc tgtgtttcac tatatcggaa agctgcaaca tgctcatcca tatcaagctg
    21661 tctttttgct tttcgaacag ctcgtttttt ttcacgataa tcaggtgcat actcaccata
    21721 tgccgctaca ttttcaaatg cttctttctt agacaagaac gcttctttca actggcaaac
    21781 ttttgcagat tcttccattg actgtttatg ttcaagataa gaagccatcg ttttactctt
    21841 ttttatcatt tcagcgactt ggaggcattg atcctccagt tgaaacagct cttcgttaac
    21901 gatcactgct atcactactt tctccgtttt cttcttgcaa taagactaac atattttctt
    21961 tagaaaaagc aatgcctaca gaatccgttc ccgggtccat gaaacggtca tggttgtaag
    22021 gatcgctgta ccaatataaa aacgaaaaag tagcgtcaat cgccggatga ataaatagcc
    22081 cactagtatt ttctaatttt tgtttactga ttaacttctc caattcatca ttcgtcagtg
    22141 agaatttcac tgtcgtttga gcactgccta gcgatttttg ccaatcagcc atacgttcct
    22201 ctgacaatat ttcctcaggc cttgtcaaaa aatcatgtag tagcagctga ctgtggtttt
    22261 gaaaggcaag tgtttgattg tatatgggaa gttcgtcttc tcttctaagc atatttaata
    22321 aagtcgtact ttttcgttct ttaacgcgat tgattgattc ccaatcagcc gatttatctg
    22381 cttgataatg tggaaggcct tcaccaaaca cttgatatgg caatagtttc aaaagactat
    22441 ctttatctaa atacacaact gaaaataggc cgtttgttgt ttgatcaaaa aataaaatag
    22501 caaaagtgtc gttatcaaaa gcaattagcg gacgataatt catatcctct tccattagtt
    22561 cgattccaac ttctgtatca tggtactcaa aagtgaaatt agtatagata gtagtgattt
    22621 tcgtaagatc ttgcatcgtc atccctaaat aaaacggcgc gatatcagca ggccctgttt
    22681 gcagtacttt aacagttgtg actctgctgt cttggatgtt cgcttcaaat gtgagatgac
    22741 tagctttatt ttcataatat cttgtttcaa aaccaaatcc gctagttaat cgatcatagg
    22801 cttggccaaa atccttttcc agctgatcga tcggttttcc gatataagta gaaaaaccag
    22861 ttgctttaag ctcctggtat ttccacgttg tcatttggtt tgatatttgt tgatcagagt
    22921 tttccttcac ttcaagtttt ggcggaaaca agactggttc catgtagaat gccacaataa
    22981 ctaatagcag aatcagccaa aacaaaccca gtcttttcat gtttccccac ctttcgctta
    23041 ttatttctca gtcacttcaa tcaaaaggtc accagacgaa atagtgtctc ctgaagacac
    23101 atatacatgt gcgacttctc cgtcaaaacg tgcttcgatg gccgtttcca ttttcatcgc
    23161 ttcagtaatc atcaacgtat cacctttttt aacgttatcg ccttttttca ctaatacatc
    23221 caaaacagaa ccagacatcg ttgctccaat ttgttctcgg tttgtcggtt cagctttcaa
    23281 tcgggtttgg accgtactga tgatcgaatt atctttgata gtgatttcac gtctttgtcc
    23341 attcaaattg aagaacagta cgcgattccc ttcgatatcg ggttctccga tttcatctaa
    23401 acgaatgata agcgtttttc cgcgttcgat ttgtacattg attgtttcac ccaaacgtat
    23461 accttggaaa aatgttggtg tgtccagtaa tgtaacatcg ccaaattgat tatatgcttt
    23521 ttgatagtcc aaaaagactt gcggatacat caaatagctt aatacttcgt cttgttttgg
    23581 ttcatagcca attttttctg ccaattcttt ttttacttcg ttgaagtcga ctgattcagc
    23641 caaaagacct ggacgttcat tcaatgcagg tctgccttta agaataatct tttgcaattt
    23701 tttaggaaat ccgccaacgg gttgccctag ttctccttgg aagaaagtca caacagattc
    23761 agggaaactt agtgtttcgc ctttttcata aatatcttct tctgtaagat cattttggac
    23821 cataaatagt gccatatctc caactacttt agatgatgga gttactttaa cgatatcgcc
    23881 aaacatcaag ttgacagtat gatacatttg tttgatttca tcccagcgat gacctaatcc
    23941 gacagctttt gcctgttgct gtagattcga atactgtcct cctggcattt catgcatata
    24001 tacttctgtt tcaggagcat tcaagccgtt ttcaaacggt ttgtaataca tgcgcacatc
    24061 ttcccaataa tggtttaatt tctgtgcatt ttcaatcgtg atttctggca gccgtggacc
    24121 attgaccagt gcataataca agctgctcat gctaggctga ctagtattcc cactcattgc
    24181 actcattgcc acgtcaacga tgtctactcc tgcttttgaa gcagcagaat aagtgatgat
    24241 tccattcccg ctggtatcat gagtatgcag atggattggc agatctgtcg tttctttcaa
    24301 ttcactaatc aaacgataag ctgcctgcgg tttaagcagc ccagccatat ctttgattgc
    24361 aatgatatgc gcacccagtt gttctagttc ctttgccatg tctttataat actgaacatt
    24421 gtattttgcc cgacttggat cgttgatatc gccagtatag caaatagctg cctctgcgat
    24481 tttaccagta tcccgtacag cttggatact tttttccatt tgtggagtcc agttcaaact
    24541 atcaaagatt cgaaatacat cgattccttg cgctgctgct tctttgacaa attccaccaa
    24601 tacattgtca ggataattag aatacccaac ggcatttgaa cctctgaaaa gcatttgcag
    24661 caacgtatta ggcattaaag aacgaatttt tctcaaccgt tgccaaggat cttcattcaa
    24721 aaagcggtaa gccacatcaa atgttgctcc cccccacatc tcacttgaaa agagttcagg
    24781 aagaccttct ccagtcaaac gtgcaatctg cttgaaatcc tttgtccgaa cacgagttgc
    24841 tagcagactt tgatgggcgt ctctaaatgt cgtatccgtc aacaacaaat tttcctgtcc
    24901 tttgatccat tcgaccaaag catcggcacc cttcgcatct aatacattct tagccgttac
    24961 gtaatcagtt cttgtgatta gatctttagg catacgtggt tcttcataaa atggtttttc
    25021 gccactttcg atacctggaa aaccattcac agtaatttca ccgatatatt tcatcgtttt
    25081 gtttccgcgg tctcgtagtc gaggaaattc aaataatgtt gctgtactat caatgaaggt
    25141 tgtttttgca tttcctgatt ggaattcagg atgtgtgatg acattgagca taaatggaat
    25201 gtttgttttc actccacgga ttctaaattc tttcaagcaa cgttccattt tctgaatggc
    25261 tgtttcaaag gtagcaccat gcgtacatac ttttactagc aatgaatcaa aatacggtgt
    25321 cactacatag cccgcgtaag catttccgac atccagacgt acgccaaaac caccaggtga
    25381 acgatacgta tcgattttcc ctgtgtctgg taaaaagtta ttcaacgggt cttctgtagt
    25441 gattcggcat tgaatggctg aaccatttaa tttaattcct tcttgctgag gcaatccgat
    25501 ttctttatgc agatccaaac cttgagcaat caacagttgc gttgttacga tatcaatatc
    25561 tgtaatcatt tctgtgatcg tatgttccac ttggacgcga gggttgactt cgataaagta
    25621 aaaatcatct ccttcaacaa gaaattctac tgtacctgcg ttgacatacc cgacatattt
    25681 catcaattgg acagctgcct ggcagatttt ttgacgttgc tgttcattca tagagacaca
    25741 aggcgctact tctactactt tttggtgacg gcgctgaaca gaacaatctc tttcaaaaag
    25801 atggatgacg tttccatgcg tatccccgag aatctgtact tcgatatgct taggattagc
    25861 gatgtatttt tcaacataca cttcatcact accaaaggct gcttttgctt cgcttttcgc
    25921 acgttcgtag ccctctcttg cactcttttc atcgtgagct acacgcatgc cgcgtccgcc
    25981 gccgcctaga gctgccttga tcataatcgg ataaccgtat tgtttcgcaa actccaaggc
    26041 atcgtctata gaagcgattg gtccatctgt gcctggaatc gaagcaatcc ccgcttcgat
    26101 agctgcagct tttgctttga ttttatctcc aaaaatatct aaatgatgca gtttgggtcc
    26161 aacaaaaatg atgccttctt cttcgcaccg ttgcgcaaaa ttcaagtttt ccgacaaaag
    26221 accgtaaccc ggatgaatag cgtcagctcc gcattctttc gcgatagaaa tgatcccttc
    26281 aatatctaga tacgcatcaa tcggtttttt tccttgtcct actaaatatg cttcatccgc
    26341 tttgaaacgg tgtacggaat attcatcttc ttctgcatag attccaacag ttttaattcc
    26401 taattctgta caagcacgaa atacacgtac agcgatttca cccctgttag caactaaaac
    26461 ttttttcatc gaaccactcc tacatctttt tttattataa acctaagctc ttgcgttttt
    26521 catccgcact gatgttgagg acaaagccga cacaaatcga aagcatcagc aaactggagc
    26581 ctccttgact taaaaatggg aacgtgatcc ctgtcaaagg aataacgcca gtaattcctc
    26641 caagattgac aaatacttgg atcaaaaaca ttgcgccgat cccaatgcac aaaagcgaat
    26701 tgaacggatc ttttgaacgg atacctacaa ggacaatgcg cacaatcata aagaatagca
    26761 acgctaatat caaaatcccc ataatcactc ccagttcttc cactacgatt gcataaataa
    26821 aatcggtttg tgcttcttgt aaaaatcctt ttttctggat actgttacct aatcccctgc
    26881 caaatagtcc tccgttgaac atggcatagt agccgttgac tagttgatgc cctttattta
    26941 attcgtctga gaaaggattt tggtaaatag caaatcgatt atagatatac tgtaaacgtc
    27001 cgggtaagat ctttccgttt gtgatattga tcagccagat cgtaaaagta cttagacaaa
    27061 agcctcctac tcctacaatc aaagtataca cataattgac tccgctggct aaaagcagaa
    27121 ccaaaataat caaaaagata actgccgcat taccaaaatc gggaagaata gcgactaatg
    27181 cagtaagtcc gataaccatt gcaagtggac gtttcacagt cccaagaaaa tctttttgta
    27241 cactgttttg tcttttagat aaagtcagag acaggtacca gatagaaatg atttttaggt
    27301 attcggcagg ttggatcgag aatcctgcaa tttgcagcca acctttcgcc ccattgatct
    27361 ccttaccaaa aaacacaaca atcagcagca aaatcgtcaa aactgcgatg gctgccatga
    27421 tcaaacgctg gtttttcaaa acatctgttt tcattttata aagtagtgcg atagcaatca
    27481 agctaagcac ccaaaaaata ctctggttaa tgactgatgc ggacggattt tgtccgtttt
    27541 ctaataatag atacgacgta gaactatata ccatgatcaa tcccaataca cataaaatta
    27601 aatagggaat caagatgcta tagtctaata gatgcctttt tttcactttc ttaggcaagt
    27661 ctattcctcc ttgccaactt cttttttatc ataaatttct tggtaaacct cactatataa
    27721 ctgattcaat tctcgttcta gctcacttaa catcagctgg ccctcgttct ttccgattac
    27781 tcctatacgg acagcaaaac cgacttgccg ggaaaagccg tacatctgtg tatctaccac
    27841 ttcttcgaag gctttgcatt gtgagataca gagactgttg ttttgatttc ggattaatcg
    27901 tttgatccgt tctgcttcat catttaatac ttggatcgca agttttttcg acaaagaatc
    27961 catctgtgct tacctcccat tctaattcct taattataac atatttgatt ttcataagga
    28021 gcaaaaaaaa cagatttaac cagttgtttt tattcatttt tacgtattcg ttctttctac
    28081 ttatccagaa cgttagagtc cgcatttttt atccgagata aaaaggcttc gccatattta
    28141 tcaagcttgc tttggccaac tccttttatc tgaagcatct ccaatctgct tgatggacgt
    28201 attcggctca tttctttcaa tgtggaatct gaaaatacga catacggggg aacgcctgct
    28261 tctgatgcta gatccgttcg cagttcccgc agcacttcaa atagtgtatc ggtttcttca
    28321 tccgatagct gctggacttt cttgggttcc tttcgataaa cagattcttg tcctttcaat
    28381 acttgtatcc ctctttctgt tactttcaaa acaggatact caccattgat ggtcagaaga
    28441 tagccattcg atatcaaata ttcaattaac tgcatcgttt ctttttgcga ttggtttttc
    28501 atcagtccat aggtagataa atgtccaaaa cctaaagctt gcagcttttg ttctttcgat
    28561 cctgctaaga ctttcatcag catttgttta ccatagttct cacccatacg ttttaagcaa
    28621 gaaagaacca tctgtgcttc tgtcgttact tctactattt ctctgtcatc taaacagtta
    28681 ccacaacggt tgcacggact gcttgtctcg ccaaaatagt ttaggatgta ttgctgaaga
    28741 caggactcaa tatacacgta ttctgtcatc gcttttaatt tttcatattc tttttgcttt
    28801 tgcccttctt ctctttggga ctgctggata aaaaactttt ggacttggat atcttgagga
    28861 gagaaaagca ggatggcttc actggaaaga ccatctcttc ccgcccgacc agcttcctga
    28921 tagtatgctt ctagagagcc aggaatttga taatggataa caaaacggac attgctttta
    28981 tttataccca taccaaatgc atttgtcgct acaatcagct gcaagcgatc gtataaaaag
    29041 gcttcttgca tttctgttcg ctcattctca tttagaccac catgatatct acccacggaa
    29101 aaatcgaatt ttttaagcaa atgatagaga cggtctactt cttttctcgt tgctgcataa
    29161 atgataccag attgattttt gttgatcttt aaatattcga ttagatattg ttcttttttt
    29221 tgatctttaa ttacttgaaa tcgtagattt tctcgttcaa aaccagtctg aatatggttg
    29281 ttttctggaa tttttaaaag tctttttatg tcagcagcta cttgcacagt cgctgtagct
    29341 gtcaaagcaa caattacagg acgatttggc agttgatcta gctggttcgc catctgtaaa
    29401 taacttgggc ggaaatcatg cccccactga gagatacaat gtgcctcgtc aatagcgatc
    29461 atacggacgt tccatcgctt cattgcttgg ataaaatccg gcatgataaa acgttcagga
    29521 gcgatataaa gtaaacggta ctgctgtcta tcgatttctt ggaagcgtct agctgtttcg
    29581 taaccatcta acgagctatt gataaaagtg gcgggtatac ctaattgatt cgccgcgtcc
    29641 acctgatcct tcatcaaaga gattaaaggg gacacaacca cagtaagccc atctaatagt
    29701 aaagctggaa gctggtaaca gattgatttc ccacttccgg taggcatgat tcctaataca
    29761 ttttcctgat caatgacttt ctggataatt tctttttgac caggtctaaa ctcatcatag
    29821 ccaaagtatt gttttaataa gtcgttcatc ttcccgctcc tttctcttgc cattttactt
    29881 cgattttctt cgaaggacaa gttgatctcc tttcgtgaaa cattatctta ttatgaaaca
    29941 aaaactctct agaaataagt tttttaaaag tcttttaaac gtctttcggc gtattttctg
    30001 aatttatgtc cattgacaaa gatgacttct ccacagaaaa gagttggaca aaaaaagaaa
    30061 ccagctattt ttt
//
LOCUS       DAGCYL010000015        29026 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.015, whole genome shotgun sequence.
ACCESSION   DAGCYL010000015 DAGCYL010000000
VERSION     DAGCYL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.015"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1030)
                     /locus_tag="MB216_000501"
     CDS             complement(<1..1030)
                     /locus_tag="MB216_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4525360.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="MB216_000502"
     CDS             complement(1112..1801)
                     /locus_tag="MB216_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBT4525361.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="MB216_000503"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="MB216_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBT4525362.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="MB216_000504"
     CDS             complement(2328..2831)
                     /locus_tag="MB216_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBT4525363.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(2951..3078)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3157..3846)
                     /gene="rplA"
                     /locus_tag="MB216_000505"
     CDS             complement(3157..3846)
                     /gene="rplA"
                     /locus_tag="MB216_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBT4525364.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(3950..4372)
                     /gene="rplK"
                     /locus_tag="MB216_000506"
     CDS             complement(3950..4372)
                     /gene="rplK"
                     /locus_tag="MB216_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBT4525365.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4715..5383
                     /gene="sdaAB"
                     /locus_tag="MB216_000507"
     CDS             4715..5383
                     /gene="sdaAB"
                     /locus_tag="MB216_000507"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBT4525366.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5396..6268
                     /gene="sdaAA"
                     /locus_tag="MB216_000508"
     CDS             5396..6268
                     /gene="sdaAA"
                     /locus_tag="MB216_000508"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBT4525367.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6359..7378)
                     /locus_tag="MB216_000509"
     CDS             complement(6359..7378)
                     /locus_tag="MB216_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBT4525368.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7551..8456)
                     /locus_tag="MB216_000510"
     CDS             complement(7551..8456)
                     /locus_tag="MB216_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBT4525369.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8515..9060)
                     /gene="nusG"
                     /locus_tag="MB216_000511"
     CDS             complement(8515..9060)
                     /gene="nusG"
                     /locus_tag="MB216_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBT4525370.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9161..9331)
                     /gene="secE"
                     /locus_tag="MB216_000512"
     CDS             complement(9161..9331)
                     /gene="secE"
                     /locus_tag="MB216_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBT4525371.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9355..9507)
                     /gene="rpmG"
                     /locus_tag="MB216_000513"
     CDS             complement(9355..9507)
                     /gene="rpmG"
                     /locus_tag="MB216_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBT4525372.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9669..10313
                     /locus_tag="MB216_000514"
     CDS             9669..10313
                     /locus_tag="MB216_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBT4525373.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10369..10872)
                     /locus_tag="MB216_000515"
     CDS             complement(10369..10872)
                     /locus_tag="MB216_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBT4525374.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10885..10931)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11469..12434
                     /locus_tag="MB216_000516"
     CDS             11469..12434
                     /locus_tag="MB216_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBT4525375.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12533..13705)
                     /locus_tag="MB216_000517"
     CDS             complement(12533..13705)
                     /locus_tag="MB216_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4525376.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13692..14837)
                     /locus_tag="MB216_000518"
     CDS             complement(13692..14837)
                     /locus_tag="MB216_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4525377.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14851..15783)
                     /locus_tag="MB216_000519"
     CDS             complement(14851..15783)
                     /locus_tag="MB216_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4525378.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15900..16979
                     /locus_tag="MB216_000520"
     CDS             15900..16979
                     /locus_tag="MB216_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBT4525379.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            16976..17605
                     /locus_tag="MB216_000521"
     CDS             16976..17605
                     /locus_tag="MB216_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4525380.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17715..17942)
                     /locus_tag="MB216_000522"
     CDS             complement(17715..17942)
                     /locus_tag="MB216_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4525381.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18045..20633)
                     /locus_tag="MB216_000523"
     CDS             complement(18045..20633)
                     /locus_tag="MB216_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBT4525382.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20653..21312)
                     /locus_tag="MB216_000524"
     CDS             complement(20653..21312)
                     /locus_tag="MB216_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBT4525383.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21361..21864)
                     /gene="trmL"
                     /locus_tag="MB216_000525"
     CDS             complement(21361..21864)
                     /gene="trmL"
                     /locus_tag="MB216_000525"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBT4525384.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22085..22951)
                     /locus_tag="MB216_000526"
     CDS             complement(22085..22951)
                     /locus_tag="MB216_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBT4525385.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23060..23701
                     /locus_tag="MB216_000527"
     CDS             23060..23701
                     /locus_tag="MB216_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBT4525386.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23763..25124)
                     /gene="mgtE"
                     /locus_tag="MB216_000528"
     CDS             complement(23763..25124)
                     /gene="mgtE"
                     /locus_tag="MB216_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBT4525387.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25142..26041)
                     /locus_tag="MB216_000529"
     CDS             complement(25142..26041)
                     /locus_tag="MB216_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBT4525388.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26043..26840)
                     /locus_tag="MB216_000530"
     CDS             complement(26043..26840)
                     /locus_tag="MB216_000530"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBT4525389.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26818..27522)
                     /locus_tag="MB216_000531"
     CDS             complement(26818..27522)
                     /locus_tag="MB216_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBT4525390.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27653..28228
                     /locus_tag="MB216_000532"
     CDS             27653..28228
                     /locus_tag="MB216_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBT4525391.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28346..29023
                     /locus_tag="MB216_000533"
     CDS             28346..29023
                     /locus_tag="MB216_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBT4525392.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8547 a         6146 c         5221 g         9112 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tgttcatcat ctcttcactt
     2941 cgttcatagt gattcacaat aaaaaactct atgccaccgc agacatagag ggactattga
     3001 atatattcaa taaacgtcct cggtaggaaa ttaaggcttg atgccaccta ctgtcttcgg
     3061 tacaagttaa ttattaacct caactagctt agcatgctaa actagttgag tcaagaatta
     3121 tttttcagtt ggatcaatca acattctaat atttaattag aaagaagctt ggtcaacatg
     3181 gataccaggt ccaaatgttg ttgtgatcgt tacatttttc atgtattgac ctttagctgc
     3241 tgatggttta gcttttagca atacatcatg gattgctgca aagttttcaa ctaatttttc
     3301 atcgtcaaat gaaacttttc cgattggtac atggatattt ccagctttgt ccacacggta
     3361 agttacttta ccagctttca cttcgtttac tgcttttgtt acgtccattg taactgtacc
     3421 agttttaggg tttggcatta agccttttgg tcctaatact cgtcctagac gacctacagt
     3481 agccatcata tcaggtgttg caacaactac atcaaagtca aaccatccgt tttggatttt
     3541 ttgaaccatg tcgtcgtcac ctacgaaatc tgcaccagct gcttcagctt cttttgcttt
     3601 gtctccttta gcaaaaacta aaacagtttg tgttttacca gttccgtttg gtaatacaac
     3661 agcaccacgg atttgttggt ctgctttttt agggtctacg ttcaaacggt atgcaacttc
     3721 aactgttgca tcaaattttg caatgtttgt ttcttttgct aaagctactg cttcggcaac
     3781 aggataaact ttacttgaat caactttttt taatgcatct tgcatttttt tgctcttttt
     3841 agccattttg gttcctcctt gattgtggtt ataacggttg tacctcccac gtgttctata
     3901 tctttcaata tagagccaac tgagaagctg ctgtcttagg gtgtatagat tattctacag
     3961 tgatccccat gcttcgtgca gttccttcaa ccatgcgcat tgctgcttca acgttagctg
     4021 cgtttaggtc ttccattttt aattcagcga tttctcttac ttgatcgcta gaaactttcg
     4081 caactttatt tttgtttggt tcgcctgaac ctttttcgat tttagctgct tttttcaata
     4141 gaacagcagc tggcggtgtt tttgtaacga atgtgaatga acggtcttcg tatacagaaa
     4201 taacaaccgg aatgatcaaa cctgcttgat cagctgtacg agcgttgaat tcttttgtga
     4261 atcccatgat attgataccc gcttgaccta gtgcaggacc tactggtgga gctggagttg
     4321 ctttacctgc aggaatttgc aatttaacga ttttttctac tttctttgcc acgagacata
     4381 cctccttaag tccgtgatgt ggttaattgg agatgcatat ttctcctccc acgttttatc
     4441 tgatctgaaa aaatccagac aaataatgtg caaaacaatg cacactggta cagtatagca
     4501 aatttatttg ttttttcaag atttttttct gctattttta cattttgctt gaataaacgc
     4561 taaatttatt ctaaaaaaac aacaattttt cgttctcatt tcttctttta tgttacactt
     4621 gcgctagcat ggttcaacag atgaaagcaa acaataacac ggttttttag acccttgtac
     4681 aaagattcct acataacaaa atgaggtgga gaaaatgaaa gatttaaggt atagaagtgt
     4741 ctttgatatt attggacctg tgatgattgg tccaagtagc tctcacactg ccggagctgc
     4801 tagaatcggg aaaatcatac gtagtatatt tggcgaacag cctgattctg tagatattta
     4861 tctatatgaa tcatttgcaa aaacctatcg ggggcacgga acagatattg cattagttgg
     4921 cggcctgtta gggatggaac ccgatgatga acgtttagct gattctttga aattggcata
     4981 tgaagccggt atggaagtct tgtttgttcc taagagtgaa aaagccgaac atccaaactc
     5041 tgtaaagatg cttctaaaaa aaggaacacg aaagctttca gtcactggca tttcgatcgg
     5101 cggtggcaat atccagattt ctgaattgaa tggattcaaa atttctttaa gcatgggtac
     5161 tcctaccttg atcgttgtcc accaagatgt tccaggtatg attgctaaag tgactagtat
     5221 actttctgag accaatacga atatcggcac tatgacagtg acacgggaat caaaaggtga
     5281 aaatgccatt atgataattg aagtagatga tcctcaagta gaagaaacgg taaaaagact
     5341 caaacagctg ccaaatatcg acagcgtaaa ttatttcgat taaggagcta aaaaaatgtt
     5401 tttatcgatt gaagaattag tagatcaagc aaaagcttat ccaagtgtag cagaattgat
     5461 gattgcagtt gaaatggaaa tgagtggccg ttcccgtagt cacatcatcg agatcatgga
     5521 aaaaaacctc tcagtaatgg aacaatcaat caacgaggga aaagaaggtg ttacctccgt
     5581 cacaggaatt accggtggtg atgcaaaaaa attagatgat tatttaaatg aggcagactt
     5641 tttaagtgga gaaacaatct taaaagctgt acgcaatgct gtagctgtta atgaagtcaa
     5701 tgcaaaaatg gggttgattt gtgctacacc gactgccgga agcgcgggtg ttgttcctgg
     5761 tgtgctgatg gctgtccgag atcggcttga tttgacacat gaacagcaat tggaattctt
     5821 atttaccgct ggcgctttcg ggttagtcat cgcaaacaac gcttctatca gtggtgcaga
     5881 agggggatgt caagcagaag ttggttctgc aagtgcaatg gcagcagcag ctttggtttg
     5941 tgcaaaaaat gggactcctc atcaagcagc acaagcagta gccattacat tgaaaaatat
     6001 gatgggactg atttgcgatc ccgttgcagg cttagttgaa gtcccgtgtg tcaaacgtaa
     6061 tgctttaggc tcttctcaag cttttatctc tgctgatatg gcattagcgg gtatcgaaag
     6121 tgtcattccg ccggatgaag tcgttgctgc aatgtaccaa gtcggacgtc aaatgccgtc
     6181 gattttcaaa gaaacagcag aaggtggctt agctgttacg ccaactgcta aacgacttgc
     6241 ggaagagatt ttaaataaga gggcgtgata aagctcctta atttcgagta ttaagagatt
     6301 agttatcttc ataacaaaaa acgaagagag gactaattgc ccttctcttc gttttttatt
     6361 aatccatcaa tttcgtattt tttacttcat cgatcgtttt tgttcctgca agttgcatcg
     6421 tgatcgacaa ttctttgttc aggtgttcaa aaacagatgt tactccttct gccccaccta
     6481 gattcaaccc ataaatcact ggtcggccaa tcgcaactaa gtcagcacca cttgccagag
     6541 ctttgaagac atgctctcct cgacggacac cagagtcgaa aatcactggt acacgtttgt
     6601 tgactacttc ggcaattttt ggcaatactt caaaggacgc aggacctcca tctaattgac
     6661 gtccaccatg attggatacc cagataccat ctgcccctgc agagatagcg attactgcat
     6721 cttctggcga ttgtattcct tttacgataa ctggaagatg ggtgatttct ttgattgttt
     6781 tgatgtcatc tggtgtcaag ccttgttttg cagctgcata aatttctgcg attccttttc
     6841 cttcgccatt accacttgca ctttgttcgc tatacgcagc taagttaggc attggtaatg
     6901 gaaattggaa ttgattgatg acgtcttctt cacggtaacc accgagcgtt gaatctgccg
     6961 ttaaaatgat agctttagcc cctgcttcta ctgctttgtt caaaatgaac tcattgaatc
     7021 tgtcgtcttt actcatatat aattggaaaa actgcggtgc gcctggcgct gcatcggctg
     7081 cgtctttgat tgtcgtattt gcataagtgc tgatggaaaa gattgatcca gcagcagcta
     7141 ctcctttagc tgtatcagct tccccttttt catgtgccaa tccttgggcc gctgatggcg
     7201 cttggataat cggtgtatct aattcaattc cgaaaacaga tgtatgcaaa tcggctgagt
     7261 cgattccgcg taatatccgt ggcatgattt tctttgtatt aaaagaagtc gtattttctt
     7321 tcatcgtcca ctcatcttca gaaccaccgc gaatatagcc aaaagctcct gcttccatgc
     7381 gctcttttac acgaccttct agttaggcaa tgtttacaat atcgattgga tgttcttcat
     7441 tacttgtatg ataagtcata gtagatcctt ctttcatctt cttgattaca tataaatcat
     7501 actaaactta caaaaagtaa gcaaacattt actctcagat acttttatcg ctattctttc
     7561 caaataaatc gacttacaag ctgatctact tcttggtttt catgaagcat actatgagag
     7621 gcattttcac ctgtcactac tttttcctct atttcatttc cgcgttgctt caacaacgca
     7681 tagaccgcta atgcactgtt taacggtact gttccatcac tcaagtctgt ttcatctagt
     7741 tgaccagcaa tcaagaaaaa tcgagtggtt ataggaatat taccgatcat ttgctggtaa
     7801 tcttgatagc ggttagaaac gacagcaggc ccctttgcta actcatctgt cagagactgc
     7861 gcactatcat cgacaaaatc attaaaaggc gcgccaattg ctgttaatgt ctgaacttgt
     7921 ggcaatgacg tatcctgtcc atacgttcct aaatagcgta gtgcactaac tcctcccatg
     7981 gaatgcccca ctatgttgac tttatctatg ccgtagtttt ctttcagata caaaagacaa
     8041 gttttgatcc attctgcttg attccactcg ttatttttat tatcttcaaa caatacttgg
     8101 accatcggat tgtctgcttt tccagacaaa tctcctgttg cttgtatttg tccatctgtt
     8161 cctactgtta aaaccaattc ttttactgct tgctgatttt tttccaaacg ccgcaacatc
     8221 cctttaaagg aattttctgt cccgctgtag ccgtggaaaa aaagtgttgg aatagtggtt
     8281 ttttctatca ctttactgga ttctgccgtt ttttctttat cagaaacttc agtagtattt
     8341 atcttcgtcg tctggcaacc acttaacgca aacaaactga taaggactaa agctgctgca
     8401 gttgcacgtt tgcataaatt tttgattggt tctgtcatac acctcttcct ttccatatag
     8461 aaaaaagaaa gacttgaaag gccaatcata tgatcgacat ttcaagtctc tttattaatc
     8521 gattttatct acttgatcga aatcaagttc tgtactggtt tcacgtccaa acatatcgat
     8581 gttgactttc aatttttctt tttctccgtc aacttctgtt acttgtcctt ctaatccagc
     8641 aaatgcgcct tcgatgattt tcacgacttc gccaacttct acttctagat cattttgtcg
     8701 caggctcata ccgatagaac gcaagatatg attgacttct tctggaagta atggtgctgg
     8761 tttgcttccg gctccgtgtg aaccaacgaa tcctgtcaca ccaggtgtgt tgcggacaac
     8821 ataccatgaa gcgtctgtca tgatcatttc aacaagtacg tatcctggga atgttttatg
     8881 aacgatttct ttttctttcc catttttgac ttcagtttct ttttcttcag gaacaacgac
     8941 acggaaaata aagtcggcca ttttcatact ttgcgcacga gattcgatat ttgcttttac
     9001 tttgttttca tatcctgaat atgtatgcaa gacataccag tttttttcaa aagattccat
     9061 tgaaggctcc tcatttcatt gtaaaataaa aaaacctcag tacacccgaa gtttttcttt
     9121 cgcttcatta tagcactaaa agtacgttgt ggctagcgag ttattttaaa atccagccaa
     9181 ataagctttg gatggctgta tccattacgt aaaacatcaa tccgaaaata atagaagttt
     9241 cgatgactac taacgtatct ttacggagtt gttttctgct tggccaagtt acttgtttga
     9301 tttcatcttt tacgcttttt agaaatttca tgagacaacc tcccttgcta ctgtttattt
     9361 cgtttcttta tgtaatgtat attgattaca atatttacaa aacttattga tttccaaacg
     9421 ttcgccgctt tttccttcac tgactgattt tgtgtagttg cgagacccac aaacggaaca
     9481 agcgagtgct gcttttttct ttgccattta cttctgcccc cttgctgaga atcagactta
     9541 tacttcataa cagtatcacg taacttttta cttgtcaatt tgtgaaataa actatctatt
     9601 gttttggata aaattatgat atgattaatt tgaaattaag ctaaaatgag acaattggag
     9661 gtatagcgat gctattaaaa acacttgtat ataagaaaaa agatctgaca accgttacag
     9721 agactagtac attagaagaa gcattaaaaa ttttagaaac ttccggatac cgttgtgtgc
     9781 cgatcttaga ttcttcaggt aatattttcc gtggaaacat ttacaaaatg catatctatc
     9841 gtcataaagc aaatggcggt gatatgacgc ttcctgtcac tcatcttttg aaaaacgcta
     9901 caaagttcat ttacctggat acttctttct tcaaagtatt ctttacgatc aaagagcttc
     9961 cttatattgc tgtccttgac agtgaaaatc atttttatgg tattttgaca catagtacgt
    10021 tgcttaatat gctctctcaa tcttggagcg tagaacaagg aagctatgta ctgacaattg
    10081 cttccacagg aaagcaagga gatttggctg ctattgcaaa aatcatttca aaacacagca
    10141 gcattgccag ctgtattact cttgatatcg aaaaagacga atacattcga cgtacattga
    10201 tcacgctacc ggctaacact gaagcgaaag tatgtgaaac gatcgtcaac cacttagaga
    10261 aaaagaatta taaagtggta gaaattgaaa atttgcaaaa ccaagaagca taatcaaaaa
    10321 gcgaaaaaag aaaacagcta atctgctttc ttttttcgct ttttataatt agacacgctt
    10381 ttcaaaatca agcgcgcgat ctaaataata catcaatggt gctgaaacag ccatgacgat
    10441 aaactcactt aatgcaagcg tgccataggt tgtccaaaat gcttggccac ctgttggaac
    10501 taacatataa gcaatcagtc ccatgctgat ggtaaagaag atcgtgttca atactaaacg
    10561 catctttaca tttttcactt tgttttgcaa taaagctgtc aggcctaaag aaagcaatgt
    10621 ttggccgcca ccaaaaacca catccatgat accaaaacca aagaaggcat tatagacgat
    10681 gaccccgcct aaaactcccc aaagcaattt acggttaaag acaactaaat gattcaaact
    10741 ttctgaaata cgaaactgga tcggccctga ggatacaggc gcaactaaaa tagttaacgc
    10801 tagataaaga gccatgatga tgccatttac aacgattacg gataaccgtt gtctgctttt
    10861 cgttttttgc attttctctc ctcttttctc ctagtttttt taacgcggga tggttgatga
    10921 accgcggtta tgtgaaacaa acaataatta taacagactt ttcatttatt tcaatccgtc
    10981 ttatctgctt ttaaaaaagc aaaagcttaa acaatgtaaa cgtttgctaa aaatcgtggg
    11041 atatggtata aagtaagtgt aaggggtcat aagaattccc agtaaagcag acgaagtagg
    11101 atctgcaaga acagaattgt tgtgaaactt aaataaataa aaaaggatgt gggaatttga
    11161 gcaacctctt actagagaac ggtctagcaa gcagctgaag atctaagtgg acatgccgaa
    11221 ccttggaact tctccttacg taagtaatgg atcatgcgct tgctaggctt ttttgtgttt
    11281 ttcggtcttc ggaccttttt cattttccta gacttctgct ataataacca agaattaatt
    11341 ttgtaacaga tttttttgaa aaaacaagtt tctttcattc aaaaaataga aaaagcttgc
    11401 taattattgg ttaaccagca aacaggagta ttaaaatatc ttgttttatt gaatagcagg
    11461 aggaaatcat gaaattccat tatcatatat tgatcaatcc atcggcaggc agcggaaatg
    11521 gctataaagt agcagaaaga attttaccgg tattaaaaaa taagcatatt gactatacac
    11581 ttcattatag tgaatacaaa ggacacgatg ctgagattgc tgaaactttg gcaaaagaaa
    11641 cattgattcc ttggatagaa gaagaacaga aaacaatcga tactttcccc cttttgatca
    11701 tcgtaggagg tgacggcacc ttacatcagg tacttgatac tttctatcag atggaagtag
    11761 aattcccagt tgcttatatt ccagcaggtt ctggaaatga ttttgctcga ggggcaggcc
    11821 ttccaaaaaa tcccaaaaaa ggacttcagc tgattttagc agctcaatca ccagaaaaag
    11881 tccacataat ggcttatgaa gaaaaaataa gtgaaaaaaa aggaatagct gttaataatt
    11941 tcggtatagg attagatgca gctatcgtcc atgcgacaaa tcattcttcc acaaaaaagc
    12001 gattgaacaa atacaactta ggatcatttt cgtatttatt ctctattctc cgtgctttat
    12061 tcactcaaaa aggatttcca attctggtag atgcaggagg aaaacaatta agtttttcaa
    12121 acgcattttt atgtacagca acgaagcatc ctttttttgg cggaggaatc gcgattgttc
    12181 caactgctga tgcgtctaaa ccagtcattg attttgtcgt ggtcgaacga atcaatttct
    12241 ttaaaatctt gtggctcatt cttttactta tacagaaaaa acaattgaaa tcgaaatatt
    12301 tccatcacta cacaacaaac agattacgta tcgtttcaac aacaccgcaa catggtcaag
    12361 aagatggaga agaaatggaa aaacgacctt ttgatatcac aataaccaca actaaacagt
    12421 tgttttggtg ttaataaaag aggttttgtc agaagctaca tctggaacac cgtttattcg
    12481 gaaaaaagga ctgtgacacg acttttgtca cagtcctttt tttactatgc agttaattct
    12541 ttgcccaatt gggttgccat cgatagtttc tttctttacc caaaataaat tggatcattg
    12601 aaaacaccgc agcaaaagca actaagacac aaccattgaa ccaaaaagat tctgcaaatg
    12661 tttgattgta actgagtgtt tcactgatca agttattatt ttgaacaaac caatatgttg
    12721 gtgtgaatgc cgctattttg ctaaccattt ctggcaagta ttctgctggt acaaacaccc
    12781 caccgataaa gcaactgcct gtgataaaaa tatctttgat cccaccgctg gtttctgagt
    12841 tttttaccaa tgaagtgata caagccgaaa agctaatgat cggcaataaa aacaagaaag
    12901 tatttaaaat atgataccat accgttatat cccacgaggt gtggatcact aacagaacca
    12961 ttagaagtaa gaagccactg aacaacagca gcgagtaact aatcaaacta aaggttactt
    13021 tgcgattcaa gtttcgtttg gtaatgggcg cactctggtt tcgtttactg atctctttgc
    13081 gattaaacgc tagattgatg acgctgaaac cgctaaaaat ggatagaaac aacccataag
    13141 acaagaaatt aaaggttgtg cccgtcaaca gctttttcat ccgctgacta tatcccgcac
    13201 ttagctcaac ttctccagta accgagagtg ccatttgtgt ctgttccata gctgcatcga
    13261 ttgttttatc ttcttcttga aacagaatca gtgtttgcaa aaaggtgttc acttgctgtg
    13321 taacaactgt tttagaaaag gcatctgggc gactctgtat cttgatcgtc gccatctttc
    13381 ctacttcgac ttgttcggta aagtctgcag gcagatgtaa gataatgttt acacgattaa
    13441 aataaagagc atcatcgatt tctttttgtg aggttccatc caatgtaacg actgtttcct
    13501 tgtcattcaa atatttacga aggccatcga ctacaggcga ctcttcttca ctaatcaagg
    13561 cgattttcgc atcttgcaga gaggtatttt caacatcaat attttgcgca aaaaagaatg
    13621 tggcaacaac agtgataatt acacctatca gtaaattcga tttatttgct ttaagcaatg
    13681 tccaaatcgt tttataaagc gtcataacgc acccctcttt ccatccaagc atttcctaac
    13741 gcaaaaagga aaataaatcc gatcaaccat agaagattgg tataaaaact agccaaatca
    13801 gtgtagaaat aaagtttata caaactttcg ctgatcaagt tgacgagatt taattgacca
    13861 acaatcggaa tatgaagatc aatccaatac ttcacttgtt ccgaccccat cataccagca
    13921 aaaaagctca tcgcaatcgt cactgaaatc aagatgctat ccttttgcac cagattcatt
    13981 tttggtatca aattaccaac caatgtacct aacaaaatcg cacacaacga ccccagagcg
    14041 gtaacaatca tcaaccattg ccaacgttca ccaaagtcta cttgatagat aaaacgaaag
    14101 acagctaaca taatataaat ctctgtcaaa acaatcgtaa agcttgctgc caaattagcg
    14161 attgaaacta acgattgctt tctaggaatc aaggacaagc gaataccatt tgacgattga
    14221 tttgcttgct gatcctgtac attgcggacc ccccacatcg tcccgtagag aatcgccatt
    14281 ccaatcagag tgaagaaata aaaacttttg aaactcatat tacgacctgc atgaacttct
    14341 tgaaaagata cttcttgttc aaaaacagtc atgatgggcg cgagatcaac gttctccact
    14401 tctgccactt tactcgaaat tataccaata cgctgtaaaa attgatccat gacgtttttc
    14461 atcaacgtct gactcattcc tgcttgaccc acatggagcg aaacagatga accatcaatg
    14521 agataaaaac cagccaaatc atctgcattt agcttttcat tcgcttctac ttccgatagt
    14581 tcctgtactt caaataatgg tttatcattg acttcgactt gttctaagac tgtggtaaaa
    14641 ttttccgaaa gcgctgtttg atccgctttg attacaattc cagtattaat cgtttcaaga
    14701 ttgccacctt gatcaatctc gccaaaggcc gccataaaca tcaatcctaa aagaattgga
    14761 aaggccaatg tccaaaacaa catggattta tttcgcagca gtatttttgt gcgatattta
    14821 taaaaatgcc aaaacattat ttcccctcct ctagtctctc aaatccttgc cagtaatctc
    14881 taaaaagaca tcattcaagg acgcttcttt ggttgctatt tttccgtaac tgatcttttc
    14941 ttcttgtaag attgtaagaa tcggcaacaa tactgtattt agttcagtag ttgttacttg
    15001 gagctgacct tttttataag cgattccatc agaagctatt gttcgctgaa tcgctgacaa
    15061 cacattttct gggagtaatg ggatgtcgat cgtcaaacga tccgccgtcc gtaccatatc
    15121 ttttagagat tctttggttc ctttagcaat cacctgtcct tgatccatga tcacgatctg
    15181 gtcgcaaaga atttctactt cttccatgta atgtgttgta tagacaatgg ttgctccttg
    15241 tcgattcagc tccttgatac tttctaaaat tttattccga ctttgggggt ctacagctac
    15301 tgtcggctca tctaaaaaaa ttaaccgcgg cttgtgggca atcccacatg cgatgttcaa
    15361 ccgacgtttc aagccaccac taagttctcc tggacgaaac gaaacatatt tttctaatcc
    15421 cactaattga atcacctctt caatcaaggc tttccttttc tctttttcgg aaacatataa
    15481 accgcaaaaa taatcgatat tttcttgcac acttagttcg tcaaagaccg caacgtcttg
    15541 aggaacgacc ccaatcattg ccttgacagc ataattttca ggagtcattg ttgtcccgaa
    15601 caatttaatg gttcctttgt cataatttaa caaagaaagc atacagttga tggcagtcga
    15661 tttcccactg ccgtttggac ctaacagacc taagacttct ccttcttgta cttgcagatc
    15721 aaaatgattc aatgctacaa aatctccata gcgtttgact aatttatcaa tttcgataat
    15781 catcatgaaa aactccttcc aaatttcttg ttgatctgac tataataaag tgcaagaaaa
    15841 gaaactagtg acgattggct aaaccacaca tgacatttgt cacaatgaag gagtctgcta
    15901 tgcataaatc tcggctactc aacgaaacta gctttctact ggttatctca ttgcttttgc
    15961 tggtaagtca aggattcgtt gctatgcaag ttatctattt attgatcgcc gttttattca
    16021 ctggtgtcag ttatttactg cctacaaagc caaattggat tttccaagtt attctatttc
    16081 ttttatgtgc ctttttacct gatcttgtcc tgtttctgcc agcgacgatg cgagtgatcg
    16141 cccctgaaga aagccacctt gattacttgt ttttgttagg ttttttgttg atttcgacgg
    16201 ttggtaatca tccccccatc aaggtcgcct ttgtcgtaat tttgctgttt ttttcctttt
    16261 acttacgaaa aaaagatcaa cgaacgaccg atcttgagaa aaagtacatt gtctccaaag
    16321 atactcattg ggaaaaacaa caagagttat cggaacaaaa taaagcattg gccctgtcac
    16381 aagaaacaca attagccttg caaattgtgg aagaacggaa ccgaattgcc cgagacatcc
    16441 atgacaatgt cggtcatctg ctgtcaagtg cgattttaca aattggtgcg cttgaaatga
    16501 tcaatcaaga accaaaacta aaagaaccat tagaaaattt aagtgccacc gttcacactg
    16561 ggatggatcg gattcgtcaa agtgtccatg acctccatga gacatcgatc tcttttcaac
    16621 actccttaga gtttctgatc gctgattttc cttcttctgt tacgattgaa ggaaacttgt
    16681 ttgaaggatt gaatgaaacg cagcaaaact gcttcttaat ggtcatcaaa gaagccttaa
    16741 ccaacagtat caaacatagt catgccaaaa ccgttgtttt acattttcga acgttgccgg
    16801 ccttttatcg cttgcaaatc acgaatgatg gcaccagccc acaaaaaaat tctgctcccg
    16861 gtattggttt gaaaacgatg cgtcaacgtg ttcaagaaca aaagggacaa gtccatattt
    16921 tccaagaaaa ccagcaattt cagttaacga tcattttacc aagggaggga caccaatgat
    16981 aacagtagtc attattgatg atgatccatt tgtgacgaaa tcgttacaaa ccattttaga
    17041 aagcacgaaa gagatccgtg tgttaggcat tggtcattgt gccaaggatg cgctagtctt
    17101 atacgaaacc catcgtcccg atgttttgct gacggacatc cgcatgccta aacaaacggg
    17161 catcgatgct gccaaagaaa ttttggctaa atttcccaaa gccatccttt tgcttttgac
    17221 gacattcaaa gatgaagaat acatccgcga agcattttcc atcggtgtca aagggtattt
    17281 aatcaaacag aacttgcaag cgattattcc ttctgtcaaa tccgcttata atggacaggc
    17341 cgtatttggt aatgaaatca tcgagacttt cactcaaatg atgaagaata aaccgacgat
    17401 tgatagtcat gcttatgctt cttttagtga acgtgagctt gcaatcatca aagaagtagc
    17461 tgccgggaaa aacaataaag aaattgcaga tgccttgtac ttgagtgacg gcactgttcg
    17521 caattacatc agccaattac tagaaaagct agatttacgg gatcgcacgc aattggctat
    17581 ctatttttat cagcatttgc aataagtctt atctaacaca aaaaaacctg aatgatgctc
    17641 cttcgcatca ttcaggtttt tttcacatct tctattcaat tgtcccgcaa accatttcct
    17701 cacgattgaa agaatcaagg attcgcaggg ctgccaggat tcgttggatt gttcggcgtg
    17761 ttaggcggaa aaataggcgc cttgtctaac gttttttcta gttttgattc ttcacgatcg
    17821 tctcgttcta catcaggact ttcattcaat ttgaattctt tttccatctc atctaacttt
    17881 ttaaagtcat tgccagattc ttttgctaat ttcgctaatt tatcttcggt ttctttagcc
    17941 ataaagtaac gccccccttt tgatttcagc ttagcatatc acactgaaaa aacaaaagaa
    18001 aacgctaacc aacgatcatt tttagctatt tctcatcttt aaaatcaagc attttttgcc
    18061 ggtataaaat cgtagggaga actgttttcc ataccaatca tcggatctac taactggtta
    18121 tcgatcactt ctgctgtaag aacgaaatct ttagaaacag caggttctac ctcttctttc
    18181 ttttcttctc caacaaatga agaaaacaga tctggttcca ctgtttccat tacttggttt
    18241 ttcgtttcat tcgtcccact gtcatcttct acaggtagct tttcttttac aacccgtggc
    18301 tcttttgtat agttcccatc ttcaaatgga tcatttccga ccaaagaatc ttcataagct
    18361 gctagtttgg tccggagagt gatattcatt ttatcattat ccatgatcct tgatttcaac
    18421 gtagtcatcc ggcttgctga ttcattcgct acacaagtga cataggcttg ctcttcccct
    18481 aacaaaatca acagttcctt actgcgagtc actgccgtgt agagcaaatt acgctgaagc
    18541 atccgctgat attgatggac catgggcaaa ataaccatct tgaattcact gccttgtgat
    18601 ttatggattg agcaacaata agaaagagtg attttattcc attcgtttct tttataagtg
    18661 acctcatttg catcaaactg gataactaat tcatccactt tatcttcaga atcttttgcc
    18721 aagatgatac ccgtgatttc tcccatatct ccattgaata cattcaattc gggagtattg
    18781 actaattgga ggactttgtc tcctatccga taaacggtgt cgttccaatg tacttccttt
    18841 tttccaggtt tccctggatt gaatatctcc tgcatcattt tatttaacgc atcgatccct
    18901 gcaggtcccc gatacattgg tgctaatact tggatatctt gtgcacggaa tcccttattt
    18961 ttcgcttttt ccacaatttt agagatcaag ggttcaattt gataagcatc acttcggaaa
    19021 taggaacgat ctttttgatt ctttgtgaaa tcttttggca gacgtccttc tttgatctca
    19081 tgggcaagag gaataatact agaaccatca ccttgccgat aaatctgatt gagttccatt
    19141 ttgggaattt gttcgatttc caaaagatca tggagtactt gaccaggacc gacagaggga
    19201 agctgatctt tgtctcctac taagataact tgcatattcg tcggaatcgc tttgaataat
    19261 gtgttagcca gccatgtatc gaccatagac atctcatcaa caatcaaaag tccgccttct
    19321 aattctttcg tactgactga aggtgttttt tcccgtccat tcagccccag aagacgatgg
    19381 atcgtactgg caggcagacc tgtcgtttcg ttcattcgtt tagctgctcg gccagttggt
    19441 gctgcaagta gaataggaaa aatttcttgg gtatattttg aaggatccaa ttccagtccg
    19501 ttcaactctg caaaaagatt gacgattccg ttgatcacag tagttttccc cgtacctggt
    19561 cctcctgtta aaataaatag aggggaacgg attgcttcag cgatagcttg ttcttgcgaa
    19621 ggtccatact gtatgccgaa tattttttcg atacgccgaa tattcttctg cacttctttt
    19681 tcaggataat ggatttcttt tttacgactg agaaggcgct ggatcgaatt accgatcccc
    19741 cattcagaaa agtacaaact gttttcgtaa agattagtct cttcttgttg tatctttcct
    19801 tcttcaacaa gttggataat tacagtagca acctgatcag gctggatttc aattgggcga
    19861 cttgtttcta aaatacggat cgtttgctga agcaatgtct ccgccggtac gtatgtatct
    19921 cctgtttgaa ctgcatgctg aaaaatctga tgcaagatag ctgctcgaat tcttctcggt
    19981 gaatctgctg caataccgat ttgttcggct agattatctg cacgtttgaa cccgattcct
    20041 tctatgtctt cgactagctg gtacggattt tcttcgatga tatccaaaga ctcgttctta
    20101 taggcctggt agatactgaa agctaactga ctaccgaacc cgtaacgatt cagtcccatg
    20161 atcacttgat ccatcccgtg gtttagccga atcgtctcag caagcatttc tctttttggc
    20221 ttcgtcaaac cggagatttg ttttaatacc gaaggatcag caatgatttt ttcgatagca
    20281 tcttctccca gcaattctac aattttttct gctgttttct tcccgattcc tgggaatttt
    20341 tcgctggaaa gatacgctac gatcccattt gcagttgttg gcttttcttg ctcatatctt
    20401 tcgagtttaa gctgccttcc gtatttagga tgatccacta attctccata aaaacgataa
    20461 gtctcatttt cttgaatatc gccgaaactt ccggtgacta caatctcttt ttcacggtaa
    20521 tcagctgtgg tttcactgat gtgtaccaaa agcactttgt aaaaattact aggattttga
    20581 aaaaaaatcg cggcaactgt accaacgaaa taaggcattt cttctggctg catcggaagc
    20641 cgcctccttc ttctatagta aaggttctgg tgctgtttca gttcctaaaa aatctgtttg
    20701 gttccatact ttcagttcat atggcaataa tctgcaaggc tcttcagtct ctaaaatcgt
    20761 caaactacta ttgaacaatc cacccatttc acgcaactca cttagctctt ttcctgtcat
    20821 ccattgaatg gctgcagtta aagaagctcc atgtcctacg aataaaagtg ggccatcttt
    20881 atgtcgggca accgcatctg ctacaacagt ttcgatacgt gtgattgccg attcaatcgt
    20941 ttctccgtga aaaggagaag gatcatacag atctaaatga ttacgcaaat tcggcagatt
    21001 atccggatat ttctggtaca tttcatcgat cgactgacct tctaaagtac ccagccctag
    21061 ttctcttagc ttatctgtat aaaaaatctc taccggacga gcaagctctt tgttgatacc
    21121 ttctgctgta tcacgtgctc gctttgctgt actggaataa attgctttaa aaggaacata
    21181 ttttaccttt ttcccaaatg ctttgatttc ttcgtaactt tgtggcaaaa gcggagaatc
    21241 gccattgctc ccttgaaaac gtctttcctg attccattgt gtcttcccat gtcgggtaaa
    21301 atatagttcc atacgcagca aactcctgtc tttcttttat tttactctat aataaactgt
    21361 ctaatccagc ttgtcatttt catagtgatg cgtcaattct aaattaggga atccttgctg
    21421 gcgaagtgct tcatacacta tcattgcagc tgtattcgat aaattcaatg agcggacatg
    21481 ctcatcattc atagggatgc gtaaacactt ttcttcgttt tcacgcataa atgtttctgg
    21541 caaaccggtt gtttcttttc caaataaaaa gaagtgctct tctccatcag caaaattttc
    21601 gtcactatat actttatggg caaattttga gatcaaatgt agtttacgat tacctaaaaa
    21661 tgttaaaaaa gcttctaaat tactgtggta agtaatattt acatcattcc aataatccaa
    21721 ccctgcccgt tttagatgtt tgtcatcagt tgaaaaacct agtggctcga ttaaatgaag
    21781 cggagtattc gtggctgcac acgtacgggc aatatttcca gtgtttgcag ggattaatgg
    21841 ttcaaataaa acgatatgat tcatcaggat cattcctctc atatttatgt tcgtttctat
    21901 tttctgacaa tttgattttt gcataaaaaa aaacgtatca actcggtgtc agtcactgcg
    21961 agtcgttacg ttagtgaagc tcttctcact aacttttatc agaacaggaa cctgataaaa
    22021 accaatgaaa aaacaacttg ttgctaatat aacaccctca actttttttt gcaacctcta
    22081 ctcattaaaa actgctagaa aaacgttttc tcttagttcc ttttaataat tctatgtcaa
    22141 ttgtaatctt cttcaaagga ttcttatata agtctgcttt gattcgctct tctacctgcc
    22201 attctaaagg tgacatggcc aaaagatctt tttgattttc ctctggtatc tcaaattcat
    22261 aggttacacg ctgacgttca gttaatttca ttgcttcagc aaacttggct actacacgtt
    22321 cattggaata actctgtttt tcaggttgat tcggataaaa agcctgacgt agttccttta
    22381 aataattttc ttgtggcacc accttcaaca cttctccatc ttctgagagt acacgttgga
    22441 attcttggta atgagaaggc gaaaagatat ttaaaaccgc gttgaatgtt tgatcagcaa
    22501 acggcaaatt ggtcaaatca gctacacacc aaaatgcttc ggcagattgt tctgtagctg
    22561 cgtagactcc ttcttttgaa atatcaaatc caacagcatt ttgaatcttt ttcaattttg
    22621 aaagttcatt caagaagcta ccttctccgc agccaacatc taatagcgag acttcttcag
    22681 gtaaatgctg cgcaatagtc tcaataacgg gctgatacat gccagattgg atcattctac
    22741 gccttggaac gaacattcct tgatcgtatt ctgttttaac cgcatgggat aaaaaataaa
    22801 gtgttccttt tttggaaagg tcaaaacggt gattattttt gcatatcaag ccattatcag
    22861 acttttttac ttcttcttgg caaaccggac agcggaacag ctgatgattt tctgccaaaa
    22921 actggcgtcc tcggtcaatt ttttttaaca tagttccccc tcatttctgt tttatcttac
    22981 cgttagatgc tcactttggc aactgctaaa aatatgatac aatgcctaga agaaagtgaa
    23041 agataaagga gaagattaga tgatcaaaga attttgcgct gaaaactaca catctattcc
    23101 tctagcaatt gctagaggag ccgatcggat cgaattgtgt gacaacttag cggtcggtgg
    23161 cacaaccccg agtactggtg tgatcgaaga aacgttaagt tatgctgggg aaaaagatat
    23221 ccccgtaatg acgattattc gtcctagagg gggcgacttc gtctataacg atatcgaatt
    23281 gaaaatcatg catactgatt tgattgaagc aaagaaatta gggacggatg gcgtagtatt
    23341 tggttgtctt actccttcag gctggctaga cgaagaggcc ttagaactat tgatcgaaac
    23401 agcagaaggg ttgcagatca ccttccatat ggcttttgac agtattcctg aagatcggca
    23461 attcgaagca atcgattggc tggtagaaca tggtgttgac cgtatcttga ctcatggcgg
    23521 tcctgctgac aaaactattg tagaaaattt cccacggctt aaagaattga ttgcctatac
    23581 agatggccgc ttaatcattt tgcctggtgg tggtgtcact gcagagaatg cagcacatgt
    23641 agctaaagaa ttgaacgtaa gcgaagtaca cggaaccaaa atcgtggatc ttcagttata
    23701 atcttataaa caaaaaaaca tttgcaacag gcataatgct tattgcaaat gtttttgttt
    23761 ttttacataa aatagcccat aaataaagaa gcaatattga aataaatcag tacactcgtc
    23821 aaatcgctca aagtcgtgat aaagggaccg cttgctactg ctggatcaaa tcctagacga
    23881 tccattagca tcgggattaa actacctgca agattcgcca ctgtgattgc aaatagcata
    23941 gccatcccta tgacaaaccc caagataaaa ttatgtttcc aaaaacctac gattagaaaa
    24001 atcgtgatcc ccgtaacagc tcctgtgaca acgccggtca agacttcagc caaaacaagt
    24061 cgaacaaatc catgatcttt ctcatcagaa atcgcaagtc gacgaacagc cacagctagc
    24121 gactgggttc cagcattacc ggcagttcct gtaatcagcg aaatgaagac tgctaaaata
    24181 ctcgcttcac ttaccaagtc ttcatagtga ctgattaaag aagcggtcga catacctaaa
    24241 aacaacagag tgatcagcca aggcaaacgt cgagacgctg cctttactgg attttcatta
    24301 atttcttcca cattgacccc ggcaagtcct gaataatcgc tagcagcttc atcatcaata
    24361 acgtcgatga tatcatcgac tgtgacgatt cctaacaaat gatcatcata atcggtaaca
    24421 ggaagagcta agaagtcata atctctaaat gtttgtgcca catcttcttg gtcgtcgcct
    24481 acatgaactg aaagcactcg ttcgctcatc agatcagaaa tcattgcgtc gtcatcattc
    24541 acgattaggt cgcgcagaga aataacccct actagctgac cttcttgatt aacgacatag
    24601 atgtaataga ttgtttcagc tacatctgct tcgttcttca gcacgtacat cgcagagcga
    24661 acagtttgat tggctacgat cgaaacaaat tcagttgtca tgatcgcgcc agccgtttca
    24721 tcctcgtaat ggagaagttc cttgatttca ctggcatcgt ctgccgacat caaacttaaa
    24781 tatttagcaa tttgcttttt atccagcgta ttaagcaagt caacagcatt atctgtatac
    24841 atctcagaaa gcatatcagc tgcatagcta ggacgcattt catctaggta ttctttcata
    24901 tgttcgttgt cttcttcaat cacatcgaac atatccgcta attctttagg cgacagatag
    24961 gaatagatca gctgcctgtt ctcttctgta agagattggt agaattgccc ctgctcatag
    25021 atatgcatct ctaaaaaact gtttctgaat gcctgaatat tattttccct taaatcttga
    25081 gtcaactggg caaatctttc ctctaattct tgattttcat ccaaaccaat ctcacctcgc
    25141 cttattcttc aaaccgtctc acaactgcct ccatgtctgc ggctaaaggc tgcttgaact
    25201 ccagcaactc ttctgtaaat ggatggtaaa aacgcagatc ataacaatga agggcttgtc
    25261 tttttattcc cagatccatt cgcccgccat acatatcatc accgagcagg gcacagccaa
    25321 ttgcggaaaa atgaactctt atttgatgtg tccttcctgt atgcagctgg atatcaacta
    25381 acgcgatttc tgttagtcgc ttctctatcc agtactcagt ctgtgccgaa cgtccttcat
    25441 cagagaccat tcgcttcaat agcgaagaat aatctcgggc gatcggcaaa tcgatatgtc
    25501 catgtgccga taaatctgaa agccttccac tgactaaggc ctgatatttt ttcacaaact
    25561 tcttttcacg aagttgcata tctaattttg cgtgcgcaaa tccatgcttt gcaaaaagca
    25621 tcaagccaga tgtatcccga tctagtcgcg tcaccacatg gattacttgg ttttcgtaac
    25681 cttgtttttt ataataagcc tttacacgat tagccattgt cccatttgga tggtattggg
    25741 caggaataga cgaaacgcct gctggtttat ttactaccag aacgtgttca tcttcataca
    25801 cgatatcgat cggtgtttca tccaaaagta cggtttcatg ttctccttca gcaggaatca
    25861 caatggttac cttatcatcc ttagctagag aaaacaagac attttctact tgatcattaa
    25921 ctttgatttg accgccttga aattttattt tcgctaacag accctttgag atcccttttt
    25981 cttttaaaaa atattttact tgctgagaat gatctttctc ataatcccat gtaaactgca
    26041 tatcaatcct cactaataaa ggcatctttc acacgatgcc aaaaatgcat atgtcgataa
    26101 gaagcaaaat ggatccgttc ttctgctatt cgataataaa ttgagcggat atttgctttt
    26161 gcgacattca attgatcaac cgttacaaga taatcttctg tattttccaa ttttatttct
    26221 agccattcat catgggcaat cacgatcgga gaacccaaag tacgaaacac tcgattattc
    26281 agtgaagcaa tctctgccaa ctgaaaggca ttgatactag gatggatcac agcaccgccg
    26341 acacttttat tatatgctgt cgaaccagta ggcgtagaaa tcgtcaatcc atctcctcta
    26401 aaactttcaa acagctcgtt tttgatgtat acatcggcta ccatcgtacg attggcccgt
    26461 ttaatcgttg attcattcag tgctaaaaaa tgctggtccg gtgtttcatc caaataactg
    26521 atccgaacat ccagcaaggg ataactaaca ctttgttccc ggttcgttcg taagctgtct
    26581 actagttctt ccagctcgta atcccgccaa tccgtgtaaa accctaaatg acctgtatgc
    26641 acgcccaaga aacgcacatc attcagacaa tgactgtagt gatggaacgc cgacaataat
    26701 gtgccgtctc cgccgactga gatgaccaat tctggctcat gatcatctcg ttgaatccct
    26761 gcttgttcta aaagtgcgtt caaacgttct gtcacttcta tagtctttgc ttctttatta
    26821 tgaacgatcg ctactttcat cccctgcctc ctctctttcg ctttctttct tcaaaagcgt
    26881 tcgatagtag acatcatctg attttcctct tccatgggag aataaccgct gtgcttcttg
    26941 gatttcttca cggatactgg acatctcttc atccaactga taggcagctt ctgccgcacg
    27001 ctgaagtcgt tcactcattt cttctggaaa aacgccttga tatttataat tcagcgaatg
    27061 ctcaatcgtt gcccagaaat tcatcgcaag agtccgaatc tggatttccg ccaaaatttt
    27121 cttctcaccg ctgatcagct gtacaggata ctcgatcacc agatgataag aacggtagcc
    27181 acttgctttt ttatttgtga tgtaatctcg ttcttggaga actttcatgt cttttctttt
    27241 acggatgatc tcaacaactt gatggatatc ttctacaaac tgacacatga tccttaaccc
    27301 ggcaatatca gacatttctt cttctaaacg tcccattgga atatgccgta attgtgattt
    27361 tgttacaata ctatcgactg gcttgactct cccagtgaca aattcaatcg ggacatgtcg
    27421 attttgttca cggaactgtt tgcggatccc ccgcagtttc actttcaatt ctgatacagt
    27481 ttgttcatat ggtgctaaaa atgcttccca atctcgttcc atagctgata cctctacttc
    27541 attaacattc attgtttatt ctaccatata tgcaaagttt tttcgtactc ttgatttatg
    27601 cattgaccaa aaaaagaaaa ttaggcaaaa tagacaaaaa ggagtcgatc aaatgacaca
    27661 aatgcttgaa atcgaatata aatctatgct gacaaagcaa gagtacgaga tattcatcgc
    27721 tcactatcaa ttaacagata aagatttccg tgtacagact aatatttatt tcgataccgt
    27781 tgatgaacag ctgaagaaac aaaattgtgg cctacggatc cgtttcgttg gtcatcaagc
    27841 tgaatataca ttaaaaacac ctgctgaaaa agggcgtctc gaaacaacgg atacctttat
    27901 aacagaagaa gcagaagcat ttatcttcga aaaacgtctg cctcgttcag gcgcagtttt
    27961 tcaaaaactt tctgatttga atatcgatcc tgcttcatta atccaaaccg gaaagcttac
    28021 aacaaaacga gctgaattcc ctattgaaga aggactttta gctattgatg aaagctgggg
    28081 agacaatcta cacgattttg aactggaact agaggtaggg gatgcatctg tgggaaaaat
    28141 tgcttttata aattttttaa aacgtttttc tttaccttat cgtcctgcta agaacaagat
    28201 ccaacgtatg cttgaagcta aaaattagaa acctttcatt ttcacaaggc acttgagttc
    28261 atttttttac ttttttctgt taagttatag gatgtgaaag aatctagtca ctagttgtat
    28321 agaatctatt ttgggggaga ttcatatgat cgaaatctat ttatttgtta atcctttagg
    28381 ggaatactgt ttcgaaatgg aacaacagtt attacaattt atcgaagaag agtacgggac
    28441 accatcaaaa gaaaagatgc aatttcgctt tcttcctttg gtcaatttgc agacaattgg
    28501 ggatgtcatg caacaaaaag gaatttctca gaacaatctt gaggaacgta atcacttatt
    28561 ctcaatgact tattctgcag cattggactg caaagcggcg cagtttcaag gaaagaaaaa
    28621 aggccgccgt tttttgatca aattgcaaga agcagtcggc tgtaatgaca taccatattc
    28681 tcaagaactt gctgaatcca tatttgaaga aatcggcggc gatttgaata tgtttaaaga
    28741 agatcgtcag tctgacttcg ttaaagaagt attcttttct gatcaaaaaa ttgctcggga
    28801 aatgggcatc acaaaacacc cttctgcagt cgtttacaat tatgcctgcg accgtgattt
    28861 tggtgtttta gtagaagtaa aaggtacaag tagcctttgg agaatcacag agctgtgtaa
    28921 aacaacagat aactcatatc aagtttttaa tggtgaacat ttacatacaa atgattctca
    28981 gttccattca agaactagcc atttacactt gctttctaat taaaaa
//
LOCUS       DAGCYL010000016        28907 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.016, whole genome shotgun sequence.
ACCESSION   DAGCYL010000016 DAGCYL010000000
VERSION     DAGCYL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.016"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            131..373
                     /locus_tag="MB216_000534"
     CDS             131..373
                     /locus_tag="MB216_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBT4525393.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            391..693
                     /locus_tag="MB216_000535"
     CDS             391..693
                     /locus_tag="MB216_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525394.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            764..2818
                     /locus_tag="MB216_000536"
     CDS             764..2818
                     /locus_tag="MB216_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525395.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            2818..4650
                     /locus_tag="MB216_000537"
     CDS             2818..4650
                     /locus_tag="MB216_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBT4525396.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            5363..7261
                     /gene="ltrA"
                     /locus_tag="MB216_000538"
     CDS             5363..7261
                     /gene="ltrA"
                     /locus_tag="MB216_000538"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBT4525397.1"
                     /translation="MTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSNFKNLMK
                     FIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKDYRPRKV
                     KRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNCSTENAI
                     ADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRKMLKAPI
                     VMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHYRKNGAW
                     DKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERLKLPISM
                     EKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIKKIQYAG
                     AKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARGMTKNGI
                     YGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRKLIHQEQ
                     QAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEIECHHIN
                     PYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTYRKKAAN
                     FPISIKMN"
     gene            7378..8178
                     /locus_tag="MB216_000539"
                     /pseudo
     CDS             7378..8178
                     /locus_tag="MB216_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            8175..10706
                     /locus_tag="MB216_000540"
     CDS             8175..10706
                     /locus_tag="MB216_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525398.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            10756..11088
                     /locus_tag="MB216_000541"
     CDS             10756..11088
                     /locus_tag="MB216_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525399.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            11089..11682
                     /locus_tag="MB216_000542"
     CDS             11089..11682
                     /locus_tag="MB216_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525400.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            11845..13869
                     /locus_tag="MB216_000543"
     CDS             11845..13869
                     /locus_tag="MB216_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBT4525401.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            13869..15074
                     /locus_tag="MB216_000544"
     CDS             13869..15074
                     /locus_tag="MB216_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBT4525402.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            15090..15734
                     /locus_tag="MB216_000545"
     CDS             15090..15734
                     /locus_tag="MB216_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525403.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            15745..16551
                     /locus_tag="MB216_000546"
     CDS             15745..16551
                     /locus_tag="MB216_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525404.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            16573..16803
                     /locus_tag="MB216_000547"
     CDS             16573..16803
                     /locus_tag="MB216_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525405.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            17349..17624
                     /locus_tag="MB216_000548"
     CDS             17349..17624
                     /locus_tag="MB216_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525406.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            17666..18088
                     /locus_tag="MB216_000549"
     CDS             17666..18088
                     /locus_tag="MB216_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525407.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            18107..19699
                     /locus_tag="MB216_000550"
     CDS             18107..19699
                     /locus_tag="MB216_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525408.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            19711..20046
                     /locus_tag="MB216_000551"
     CDS             19711..20046
                     /locus_tag="MB216_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525409.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            20193..20387
                     /locus_tag="MB216_000552"
     CDS             20193..20387
                     /locus_tag="MB216_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525410.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            20419..22791
                     /locus_tag="MB216_000553"
     CDS             20419..22791
                     /locus_tag="MB216_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525411.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            22852..24312
                     /locus_tag="MB216_000554"
     CDS             22852..24312
                     /locus_tag="MB216_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBT4525412.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKD
                     ICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            24323..26527
                     /locus_tag="MB216_000555"
     CDS             24323..26527
                     /locus_tag="MB216_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBT4525413.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            26548..26697
                     /locus_tag="MB216_000556"
     CDS             26548..26697
                     /locus_tag="MB216_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525414.1"
                     /translation="MRNTTHKEQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     LELDR"
     gene            26773..27366
                     /locus_tag="MB216_000557"
     CDS             26773..27366
                     /locus_tag="MB216_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBT4525415.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            27382..28281
                     /locus_tag="MB216_000558"
     CDS             27382..28281
                     /locus_tag="MB216_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBT4525416.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            28284..28769
                     /gene="ssb"
                     /locus_tag="MB216_000559"
     CDS             28284..28769
                     /gene="ssb"
                     /locus_tag="MB216_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBT4525417.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
BASE COUNT        10883 a         4638 c         5953 g         7433 t
ORIGIN      
        1 ttccctatga gaaggatggc gtaaaaaaaa taatttttct tggatctagc aaggatgaga
       61 agcgaacgaa agagaaatta attcatgatg gatcacaatc aaataaatac aaattacaaa
      121 agactagcga gtgattgcta gtctttttat ttgtcctgaa ggaggaatca cagtggcaaa
      181 agctaaaacg ccatttgaaa aagccgcgtc taaaatctta gaacgccgcg gtgtatctga
      241 aacacagtgg aaacaagaag tccttgaaaa agcacaattt gacctattac ggggaaaaga
      301 caaggactta gaaggatata tctatcaacg tgaacgtgaa aagcttgttt tcgatgaaat
      361 tattaaacac taaaaggagc gaacaatttt atgtttgaat caattacaat gaatttttta
      421 acacaaccga aagtagtagc tgcggttgat atttttagag cattacaaac tgccgcaact
      481 agtttgaaaa ataatctgat gatttttgct gctgttgcgg cagtggcttc cattgctctt
      541 ggaggtattt tcttcttctt tgggcgaaga ggagctgaaa acgggaagac acacgtaggt
      601 tatgtattgt tgggggtagg agttatttta gctgccgctt ctattgtagt ttcaatgttc
      661 tcaatgtttg gacaaacccc acaaggacta taaaaaaaca agtagaagga gtaaaaagta
      721 cgtggctact ttttactcct ttttttatgg aaggagaata ttcatgtttt ttacgatttc
      781 aggcaatgca aaattggata aagagttaat aagaaaagca acgaagaaag tgggaaagcg
      841 tacttccttt ttaccgaaaa caattaaata ttctgtcatt gaaacggaag aacagctaca
      901 tgtgttagga caagtgttag aacaagagaa tcaatcttct gttaaattga ctctcacgtt
      961 acatgataaa gaaggaaaaa tagaaaaggc tggcacagaa tacgtttttg gctcactgga
     1021 ttatcaaatt tcagaaggtt tttatcctac attgattgat ttaatccgcc tagatatatt
     1081 taaagatacg gatgaaacca tgagtgtgga agagtcttat cgaaacgaaa aaattttaca
     1141 agttttagaa gaagagttag aaagcgacca aagaaaagtg aatcaagtga gtgaagtcga
     1201 agcaaacgaa gaagtaccat gggaacgccc aatctttcca aaggatgaat caaaagaaaa
     1261 agagaaagga attattgacg aacaagctga cattttgact cataaatcaa tatttgagga
     1321 agagaacgag gtaatgggtc aattggaggt agatcccttt gaagaaacaa tggaggatga
     1381 agagacggcc gaagtagaag ttgatccagt tgaagctaat gaagacccaa catggactga
     1441 aggggattct caggaatata ttgaaccact tatcagtgaa gaagtagaga aaaatgaaac
     1501 agagatacaa aaggcaacgg tatttgatta tattttggat caatatccgc cagagcatga
     1561 atggatgaaa gaaaaaaata aattattttt aaatgaactt tattcgaaat atcaattacc
     1621 tcaaacctat caagcatttt tagagagtaa agaagaatta atctcacagg gtaaaggcac
     1681 acttttagaa cagttagagt ctgtccaatc aacaaattgg catgagcttg cacaagaaga
     1741 tttattagct acttttgaag aacgtcaaca agaaacagat caagaaatta atgagtatgc
     1801 gacggaacag tcgtcgaatt gggagaaagc aaaagaggaa ctaaataaag aagaacagcg
     1861 agtgattgaa gaagaagtac aaaaaattaa agagcggtac gaaaagaaac gtgacaatgc
     1921 gtattccttg tgtgtagaaa agatcaacaa ttttactcag acgaatcaag aagcattatc
     1981 gaaagaaaaa gaaaacttat taaaagaacg tataaaagaa cgaaaagaag aattttaccg
     2041 agggttgcgt actgataagc taaggattag caaagaatta aatgaacaac tgaataaact
     2101 ctatcaaact acgactgaag ttctgatgga aaaacacgag cagatccaac aagaattaca
     2161 agcacaaatt ccaaaatgga aaaaagagta tgaaattcac caacaagaat tgaaagaaca
     2221 agaagaaaaa caacggaaaa aagaaaaaga gcgcgaacaa ttagaactaa aaaaacgtga
     2281 acaggatctt gcggaacaaa aaatggcgat agaaaaacaa cggctagaac aagaaaagga
     2341 aaaagaagca gctgctcgta aggaacgtca agaacaaatg gatacattgc gacaagcaca
     2401 gttaaattat ctgacacatc ctcaaccagt agctgctgtt caaacgccag tgaacaacca
     2461 agcaaattta actccttcac cccagcgttc ctttaacgga tggatggtgg gatgtattgc
     2521 aagtttaagc ttgcttctag gtggaggaac agtatttgct tttaatcata tcaatcaatc
     2581 ttcacaggct gattctgtag ccacaacggc gtcaatcaag gaagaagccc aggaacaagc
     2641 aagaaaagag tatgaacaac aattagaaga attattgaat cgtaccaatc agtcaacaac
     2701 agagtcaagc atggaaaata gcaaagagag tgattctagt aaggctaaag aaggatcaag
     2761 cgattcaact gtacaatcaa acacttccga aagttcagaa tcaaatgagg tgaaataatg
     2821 cgtgttagag aaacaacgaa gcgaaaagac aaacgattca atttagaaaa gaataggtat
     2881 ctttctcatt tctatcgcta tactccctca ggagaagagc taccagaaaa acatgacaaa
     2941 cgcatttcga aaaagatttt ttatagtgga cttgtatacc tccctttaga gtatttagta
     3001 acaagagtac cagaagtttt caaccgattg gaaattgtta agggaagctt acaaggaata
     3061 aatctgtttc aattacagga aatgccacag gtctttcatt ttgattcagc tggtttttgg
     3121 aatccattta ctcctacaac tagtttgggt acacgtttag caatttcgac agtttgtgtg
     3181 gttccagctg tacttatggg attgaaaatg gacatgtggt ttcgtcctct agcagatgga
     3241 caaaaagggg ataatcgatt aatgacctta aaagaaatcc aacagattta tccgcaaata
     3301 cctgaaacaa aaactacttt tccaggttat ggaggtattc caatcacaca ttacaaaaaa
     3361 tattggtaca ttcaaacaga tactgttaat acgtgtattg ttggtacctc tcgatcaggg
     3421 aaaggtcaga ttgaagtgtt agccaccatt gacaatttat ctcgtgcaga aaaacaaagt
     3481 tcaatggtcg ttaatgatcc aaaaacagaa ttatatgttg gtagtaaaga tgaactagaa
     3541 gctagagggt atgatgtata tgcctttaat atcaccgatc cactgcaatc aatgagtgat
     3601 aatccattgg cactcatcgt gaaatactgg aaacgaggag acatagatac tgcaacacag
     3661 ttaaccaata ctttcacaaa tacgatttat tatgatgcca acgcttcaga taacaaatac
     3721 tttaacgaaa acgcgcagaa agctgtcaat gcgcttattt ttgcgttgtt agaacaagct
     3781 gatcaaacag gagattactc caaattgaca ccgaataatc tcgttgagct tctttctgag
     3841 attggcggtt ttaattatta tccagatcca gacaatgaat tcaaacagtt gaatgcctta
     3901 gatgaattta tgaatcagtt gccacctgga aacattgcaa aaaaacaata tggggcaacg
     3961 aaaattggat ctgataaagc gaagggaaat attttatcaa cggcaatcac gggattgaat
     4021 ccatttactc taacgaaaat tgcaaagatg acttctcaat caacgattga ttataaatcg
     4081 gttgggtttc caaaatactt agatttgaaa ttacatgaaa gtcttttaaa tcaacgagtc
     4141 gtagtagaat tttatcataa gggaaagaaa tttcatgaag aacgtgtcaa agtaggctat
     4201 aaaggattta gtgaaatcaa ttttgattgt catttagttg caggtgatca agtttgttta
     4261 aaatacgatc atcaaggaaa agaatatgtg gcttggtatg aattttctca aagagtattg
     4321 aaagacgaag agggtaatat cgtttatcgg aaaaaacgag gagaaaccca tttgccagaa
     4381 ttagaaaaag aagccgttct aacattagat gaggaaaaaa gtaacttatt agtagagtct
     4441 attcggatgc actataccga taaaccaatg gctattttta tgcttacacc tgattatgat
     4501 ccatctaacc atatcattgt tagtattttt cttagtcagt tatataaaga attatctacg
     4561 caatgtgtta agactaaagg agataaatgt caccgcagga ttcattttat tttagatgaa
     4621 tttgggaata gtgtgacctg tttagcctaa ggataaaaga ggctagttcc aacgaacatt
     4681 ggaataactt tactatcgag aaatcaaatg acggggtaac gccctgaagg gtttccactg
     4741 atactgcgac atgcgttagt aggatgtgca tccaaacgcg tatgaagctc gctgaagtcg
     4801 gcagaagggt accgtaagga aagacacgat acgtatctac cacgcaagtg ctctagaggt
     4861 agggtatgta cctgatatgt cgggtgtcct aaaaagttca ataaggtgag aatgatttga
     4921 ttatctgaca aacttgcgaa tggaatgtct gaaacgaata actcatagaa atgtgagggg
     4981 gaagggggat acgtcctctt tccattgact cgaggttgag taaaaatgtg tttctatgaa
     5041 aaaccttgca tggttaaagg ctatggagta gtcaacaggc aaaagcaagc acctaagttg
     5101 aaatctatgg atagatagta aggaacaggg gaagcatcta gcacggaggg agcataccga
     5161 agatgtgttt ctaaggaaaa accgaaatcg gttataactt aggtgaatag atgtgaaagt
     5221 agagccacga ccagtgaaaa tttcgtaatg aaattggagg aacaggctca agtcgttatt
     5281 tataatataa gaaacctatt taaaatcatg gattctcgta agactaaaaa ggtcacgaac
     5341 cagaatggag cgtgataaaa ccatgactaa cacaaaagga attagcttaa agaacaaaca
     5401 actcagacac gccgaatact acgacatgac tgagactttc gatgaattgc acgcaatgag
     5461 tgttgcaaaa tcaaatttta aaaatctcat gaaattcatt attgatgaca ataatatctt
     5521 tctagcgtat agaaatatca aaaagaataa aggaagtgca acggccggat acgatggtgt
     5581 cactataaaa gacattgaaa aattggcatg tggtgacttt ttaactatta ttaaaaggcg
     5641 atttaaggat tacagaccaa gaaaagttaa aagagtagag atcccgaaac caaacggaaa
     5701 acttcgacct ttaggaattc cttcaatatg ggacaggata gtccagcaat gtattctaca
     5761 aattttagaa ccaatatgcg aagcaaaatt taataagcat agctatggat tcagaccaaa
     5821 ttgttctaca gaaaacgcca tagcagattg tcttgctaga atcaatcaaa gtaagatgaa
     5881 ttatgttgta gatattgata taaaaggatt ctttgacgaa gtaaaccata ccaaacttat
     5941 gcgtcaaata tggacaatgg ggattcgaga taaacaactc cttgtaatta ttcgtaagat
     6001 gttaaaggct ccgattgtta tgcgaaatgg caatgttgtt tttccaacca agggtactcc
     6061 acaaggaggg attttaagtc cactgcttgc gaatatatgc ctgaatgaat ttgattggtg
     6121 gatagctaat caatgggagg aacgaactgt caaagagctg aaacaccact atcgaaaaaa
     6181 cggagcatgg gataaaggaa atatctattc gaaattaaga agccacacaa gtttaaaaga
     6241 gatgtatctg gtacgctatg cagatgattt taaaattttc tgccgaacag gagaagaagc
     6301 ccaaagggct ttttatgcca cacaaaaatg gctcagagaa agattaaagc tcccaatttc
     6361 aatggagaaa tctaaagtta cgaacctaaa taaagctgaa agtgaattct tgggtttttc
     6421 aataagactc cacaaaaagg gaaaaaagaa agtggctttc acgtacgttt gtcgcaaagc
     6481 agaagaaaac attaaaaata aactaaaaca acagattaaa aaaattcaat acgctggagc
     6541 aaaaaaaaat aggcaccgag caataggtga ctacaacatg atggtcatcg gtatccacaa
     6601 ttactatcgg atagcttcac attgtagtgt caatttcact gagatttata gaagtgttag
     6661 gacagtaata gaagcaagac tgaaagctag aggaatgacg aaaaatggaa tctatggaca
     6721 agatcatgaa ggataccgtc cgtatctaaa gtctaagcaa atgagatata ttgaaggttt
     6781 tccgatactt ccaattggct atattaaaaa aagaactcca ctcaacaagc ggagaaatct
     6841 aaataaatat accaaggaag ggagaaagct cattcatcaa gagcaacaag ctatagaaag
     6901 ctggaaaatc aagtatcttg taaaaggttt tagtaacaat aagaaataca cggtggaatt
     6961 ttctgataac aaaatatcta agtttatttc ccaaaaaggg aaatgcgcta tcacaggaaa
     7021 cgaacttttg atggaagaaa ttgaatgcca tcacattaac ccatatcatt tgtccaaaga
     7081 cgatagttac aagaaccttg tcattatctc tagagaaatc catagattaa tccatgcaac
     7141 tagtgaagaa actattctga aactgcttcg gttactgaaa ctaaatcact atcaaattag
     7201 aaaactaaac acgtatcgaa aaaaagcagc taattttcca atatcaatca aaatgaatta
     7261 gataggaaac aaattatatt gtaattaacg atggaacgcc gtgtgcggtg aaagtcgcac
     7321 gcacggtgtg gaccggggga aaagacggag ataatatcaa actcttacct atcggaattg
     7381 ccaccaatgg ataatatgga aggaatcatg acggtcacag ctggccgaaa catgttgtgg
     7441 gatctgttta ttcaatctta tcagcaactt tatgcgaagt atggagaaca aaacgcagcg
     7501 attatcaaag aaaattgtca gacacatatt tatattatgt cgacagatga taataccatc
     7561 gaagaatttt ctaaaaaagt ggggaataag acggttgaac aagagaacag taccatcaat
     7621 tcattaggta tgaatactca tatcaatcgc aatgttgatt cagatcgtat cttaactcct
     7681 gaaagaattt ctactttttt agaaggtgaa acgattgtac tcgctccttt aaaacgacaa
     7741 gatttgaaag gtcataaaat acggccgtat cctattttta atcatgggga aacgaatctt
     7801 ccttatgctt atcaatatat tgaagatttc aatccatcgt gggatatcaa tgatatagac
     7861 atacattgta cacatgcgga cttggatctt aaggctttac aaattgatta tgattatttc
     7921 ctaccagatg aatatgctag aaatctctat catggactgc atgatcaatt agaagaaaaa
     7981 aacgaagaaa atatgtttta taaaatcgtt caatctcttg agaatgagac ggctaaaaaa
     8041 ttgttaattc aattttacga agaacaagca gcagatcgac ttcaagaata tgttgaaaag
     8101 ttgatcaaaa atgaagaaat ttctgaaatg attgggaaac ggttgttatt ggaatctgaa
     8161 aaggtggtga aagaatgatc attggaggaa ttttttctgg catagccgac tggtggtcag
     8221 gcttattcgg agaaggggat gcaaacccca aaaccgtaac gaaagtctta caagattatg
     8281 gtaattatct gcagtatcaa gatgtgttca gttggatttg taatactttt ttatgggggt
     8341 tgatcaaagg gttatataat ctcaatactt tactagaaaa aaccatttat caaagctttg
     8401 atttaaaaga cttattgaac gccgctgggg taaatgaact atttcaaagt ttgattagta
     8461 aggtattggc tcttttttgt gtcgtcacat taatttattt aggcttgaaa ttttttatga
     8521 gtaaacaccc accaaaagtc aaaaatatct tggttaattt atttatggcc atgatcttga
     8581 tttttggtgg tagttcttta atcgatgaag ggttaaatat cagtaaaagc ttttacggag
     8641 acgtcactgc agcaaataaa gcagataaaa cgggttcacc tgcttttcaa attattcaaa
     8701 ataatgtgat cgatgtcagt acattactag caactgaccc gaataaagta gaatccctgc
     8761 caacagacaa aagaaatgct ttaacagcga aaaattttaa aacagcgaat atcaatggga
     8821 ttattactcc taaacaagct aaagaaatgg cggataaaga taagagtggt ttatcagatt
     8881 tacaaaagga tcggttaaga gcgttacaat accgactgga actggatgat aatggaaaag
     8941 aaattcctgt gcaaatcagt gatgaaggct tagccaaata tgtttatact tcagggtatc
     9001 gtcgatacat tagtagtcca gggattattt tagctggtga atcttctttg gcggtggcct
     9061 atttatttat cctatttacg atcgttactt gcattattga gctggtattt aaaaaattct
     9121 atttagtgat tgccgcgtca acggactttg agacaggaca acggatgaaa agtgctatcg
     9181 aagatatttc tcaatccttc ttgttattgg catttacaat tttagaatta cgaatctata
     9241 ccttaatgct ttcaggtatt ggtgatttgc atgcggcagg aaagatcaat ggattcttgt
     9301 atgttgtcgc tttgattgta ctaaccattg cgctattcaa aggctctcaa tcagttacga
     9361 aaattttcgg cgttgatacg agtctgaaaa acggcagtaa ttctcttctt agcatgtttg
     9421 ccttaggaaa tattgcgaaa aatgtgggta gcggagctaa aaaccttgca agtgctggta
     9481 agagtggttt aagccattta aatgatattc gtaaaaatgg atttggccat cgaaaagatg
     9541 ttacccctga taacggggaa gaacaatcag acgataaagc tacaactgta cctaatagta
     9601 agaaaacagt ttccgaaatg ttcgggaaag cgaaaaatgg tgtttctaaa gccgcagaag
     9661 gtcttggtta cgtcaatgaa cgtgggttaa aaggaacagc tgaagatctt acagaaaaag
     9721 ccaaagacac cttagaagat aaattagacg gcacgagagc taaagaattg gctgaagctg
     9781 tgaagaatcc atccgaagca ctagaaaaac aaaaagaaaa agcttcagaa gtcaaagaag
     9841 gactaaaaga tgcgtatggt gaaggacaag tcaatgcagc agtgaaagct aatacaacaa
     9901 ccgaaaaaga aaatcaagaa ggtatagtga ttccttcagt caaagatcac caagaagcgt
     9961 tgcactctaa tgaaaattcg attgaagaga aaaattccat agaagaaaga aattcagaag
    10021 aaggaaaaaa tggacagtta ccaaaaactg atccagaaca atatcgctat aaacctaaca
    10081 gtgaccttga aaatgagaag cgaatttcag atttatcaaa agtaaatcaa gaagacaaag
    10141 cagaaaatga gggacagtta ccaaaaactg atccagaaca atatcggtat agatctgtta
    10201 actccgaaaa tgaaaaacgc atttcagatt taccaaaacg aaatcaagaa gtacaaacgg
    10261 atgaggattc catcgttctg ccaaatgata atggaatcga agttggaact gaaaaatcaa
    10321 cgaaaattgg tttacctaag gcacaaaatt taccaatgaa agatagtcgt ttgaatcaat
    10381 tcaaggataa tgatcaaagt tttgttcatc aatcacctgc aggtactgtt caaatgaaac
    10441 caagtcgttt gcctgaaaat ggaaatctga caactgtttc ttacaattta tctgaaagtg
    10501 gtgagacagg gaatgcgtcc gttcaaaatg ttgtgacaag agaagctgca ccaaaaccta
    10561 ttcaaaacgt tcatacaggt tacaagccag caaatgtggc atttactgca ccaacaaaaa
    10621 taaatgtgac aagagaaact aaaaacagga acaatgcatt gaaagaacaa ctattgaggg
    10681 atatcaaaca ggatatcaaa ccataaaagg cctacaagtt aggccttttc tttatatgaa
    10741 aaggagaaat caattatgga taaatatata aacatgaaaa accctccaag atttagtgga
    10801 gaatggaagt ttttcgcttt tttcttttta gatctattaa cgcttggtgg ctgctttttt
    10861 tgggcgaaaa tggtcaataa tcttgtccac ttaccactct tttttgcact cttgaattat
    10921 ggcttatgga ttgcgatagc gttagtgatt gtttggcgac cctataataa cccaggtcaa
    10981 cgacaaattt ttgcgattat cgagtctatt ttttttgcgg acaagaacca ttatcatgcg
    11041 ttagatccta accgaaatta tcatggtacg tataaggagg atgaataaat gcgagtcaaa
    11101 ggacaaaatt cagttgaaag gaaatccatt ttgcagtttg gaaaaccaga agagcgctct
    11161 atcttggact tatttccgta taaatcctgt acagaagagg gagtcttaat tactagagaa
    11221 gatcattttc aacgttatta tcgtgtagtt tctacagacg tggaaggatt aaatgaacaa
    11281 gagaaactag aacgaatgaa tcagttgacc accgtcatgc gtacgtatgt gccaacaatt
    11341 aagttagtta gtttgaccac agagacggat ctaacggaac aaatcgtgca aaagcggcag
    11401 ttattagata aaaatcgtat ggaacagtcc ttaggaagaa atctacagcg attaagaaaa
    11461 tatgaaaaga aattagtaga ggaaatccag gaattgaaac gagcagaaca ggaacgccct
    11521 gatttaagct tctttttcgt cattgaagga aagacaatca aggatttgca aaccagaaac
    11581 cgtcagttaa tgcgatctag tggcatttta gggttaaaac cactctacaa aaaagaattg
    11641 atcaagattc tttatcgaat gaataatatg aatgatgagt aggaatgggg aagagagaat
    11701 gaaatatccg ttgatatggt tagaaaaaga tgttccgaaa gaagagacca ctagaaaatt
    11761 tcctcacact ttaagcctgg gtaagttggg aaaaggaaaa tcttttaacc aagtaaagaa
    11821 tcaaacaaga agaggtgact gtacatgtta gaagcaatta aaacagcttt ttcaccacaa
    11881 aatgatcaaa aggtacttga tccattaaag caactaggaa atcgggaaat agaaatttta
    11941 aaatccaaag ggtatgattt agacttttta aaacaagtga tgccacaagg tggattaaat
    12001 ttcgatgaag agtatgtgac ctatggaaat ggttattcac gatgtgtgac agtttatcac
    12061 tacacaaaaa atcctacgct tttttggttg gccaatctca tgaacaatcc tcatacagtg
    12121 gcaacactag atgttagcac agatgaaaag caaaaagtga ttgatcaaat caatcgtgcc
    12181 ttagatgaat taaatgatcg gtcagataat gaacgaaaag cgactgaccg aaataaaagc
    12241 gcggaagaat acctagaact agcggaatat gcccgaaaat tgacttctaa tggggaaatt
    12301 tctaaacaag tcaaagtgag agtacatgtt tacggaaaca cgattcaaga agtagatgaa
    12361 cgagcacagg aactgatcga aaatctcaaa gggatggatt atcgtgcagt gacctatctg
    12421 cacaaaacga aaagagactg gcaatcgatg ttcaccatgt tcgatgaaca agctgaatgg
    12481 tttggaaata tcaaagggca atcactacca tcgctaaaca ttggcggagg tttcccgttc
    12541 caccaccaaa cgctaaaaga tccaggtgga acacaatatg gggaaaccat gacaggcggc
    12601 ccgtttgttt ggaatgccac gtataaaaat agtgtgcggt tgtctaccaa taccttgctt
    12661 ctaggaatga tgggagctgg taaatcgacc gttcttaaaa tgatcgcgga agcccattta
    12721 gctgcaggag actttatttg gggctttgaa aagggaaaag actttattcc ctttttaaag
    12781 gaatataacg gcattatggt gcgtttagac ggatctgacg ggatgatcaa tcctttagaa
    12841 atatttgcca cacgtacgta tgatgaagct tccagtttat atgacgatgg atcagtcaaa
    12901 gatttgaaga tcaatgaagc agcgtcctat caaacgcact tagacaaagt ggtctatcag
    12961 gtacagttag tgagtcctca gctaaaagga acgatgaaag cagaatttaa gacctattta
    13021 aatcgttttt atgaagagta tggaacagta ccacgcggct ttacttcctc aaattcgcgt
    13081 tcaaatactg atacgcaagt cacaggtaaa gatccagaag cttatccgac gttcaaagaa
    13141 tttttagact ttttaggaca actggaatta ccaggtgctt cccaagagaa aaagaatcga
    13201 aaagaagaaa tggaatccat cgtagaatcc ttatgtgaga cgtatggcat gatttttgat
    13261 ggtcatagta cgattcgaca ccttgaccaa caacagttgg tcttttttga cattgactct
    13321 attacaggct tagatcgagg ggtacaacaa tgtcaaatgt acatggctat gacgttgatt
    13381 tggaatcagg cattgattca aggaagaaga caacgacgtt taattaaaga aaaacaaatg
    13441 gaagaacaag acttcaaatg cttcttggcg atgattgatg agtgccataa tgcgattaat
    13501 ccagatttta ttgaaacggt aaattatgtc acaaactttc aacgagaaat gcgtaaagta
    13561 gaagcaatga ctgttttagc tacacaatcc ccacaagaaa tggtgccaga aaatatgtct
    13621 tctgataact tctcgaaact gaagaaagtc tttgaatttt cttctacgaa gatctttatg
    13681 cgtatggatg aatctatttt acgtcatata gaaggattag ttggtaaatc aatgacaaac
    13741 tcagaattag aatccattcc tcaacaaaat caaggagacg ctgtgatcaa ttttggctca
    13801 aaagaaacca ttcgtgttca ttttagtccg aacaaacgtc aattaaaatt gtttgatgga
    13861 ggaaaataat ggaaaaccaa aatgaaagtt taatcaagca gtatgttaag cgtcgtgcaa
    13921 agcgacgctt atttttgtgg ttgtttggta cttctgcagg tttgattact attttgatta
    13981 ctgtgttcgt gacccttttt ctaatacttg cggcgggttc gattgataat tctgattcag
    14041 attcaagtag tggtggcgaa gcttttacag gagaatattc ggaaggctta ccgatttata
    14101 aagaaatcaa aggaagaggc cctttttctg atgagattgc gcaatatgct gtaggtgcag
    14161 cagtgaaata taaattatta ccaagtgtga tcctttcgca atacggttat gaatcagcgt
    14221 ttggtacgtc ggcgtccgca agaaatgatc tcaactattt tgggattact tggtttgatg
    14281 gttgtctgtt tcctaaagga actgcgcgag gaatcggtgg tattgaaggt ggctggtaca
    14341 tgaagtttcc caactcaaaa gcagccttct cctattacgg ctttatggta gccacacaat
    14401 ccaattttaa tgcatgtgta ggaaataaaa gccctggcgc aagcttattg atcttaggac
    14461 gaggtggtta tgccgctgca ggaattactg aagatagtcc gtattataca ggttgtatgt
    14521 ccattatcac ttctaataaa ttaaccgaat atgatgaatt cgccatcaaa cattggggtg
    14581 aaggtggcaa taacaacggc acgatcactg gtgaatggac gaatccattc cctggcagca
    14641 gtttagataa aagttccttc agtggtggtc aattatttgg aacaaatcca ggcggtgaat
    14701 tccgtccgaa tggcttccat gatggattag actttggttc agtcgatcat ccaggaagtg
    14761 aaattcacgc ggtgcatgga ggaaaagtcg tgtacgttgg caatcctggt atttcaggtt
    14821 taggtgcatg tgtgatcgtg attaattacg acggtttgaa tatggtatat caagaatttg
    14881 cgaatagtac aggaaattct cgtgtgaagg ttggcgatca agtgaaagtt ggtcaagtaa
    14941 tcgggatacg agatacagca cacttgcatt taggtttcac tcgaatggat tggcgtcaag
    15001 cacaaggaca cgcattcaca gacgatggta catggattga tccattacca tctttgaata
    15061 gcagcaaaaa atagatcagg agaaaaaata tggaaaataa aactttaaaa atcgtcttaa
    15121 cagctgcaat tagcgttagt ttaatcggca atatcgcgtt gttttggcag ctgaaagaag
    15181 aacaagagac aaataccttt aaaattgaag aagtggctaa aaacgcgtct gagaagctca
    15241 aaaaaaattc ctctgaggca caggcgaaga tcgacaagtt aaatcaaacc atcaatgaga
    15301 aaaaccaaga aatcgatcag ctgaagcaac aagatggcac aaaagcaaaa gaggaagtaa
    15361 ctgaagcaca aagaaagacg gcagaagagt tcggtcaatt ggccattgat aaatcattaa
    15421 gtagaaatga attaacggaa aaactaaaag atgtcgcaac ccaagaagtg atcgacaaat
    15481 taagtcctgc agacgatgat caccaacatg atgattacgg aagttactcc tttacattag
    15541 gtgacgtgca aacttatgct caaacaagca gtgtaaaaga ggaacaaaat tttgtcgtat
    15601 ttgttgatta tacaatcggc aatccgcaat tcaaagatgt caaaccacaa aaaataaaag
    15661 gtggattaac tgtcactgaa aaacaagtag atggtcaatg gaaagtcata gactttagtt
    15721 atttcactcg ttaggaggaa gaaaatgagt aagaaaaaag gagaaagaca acgtttcaaa
    15781 aaacgaaaaa gttatcttca atggctggga atctttatga ttctcggctt tttgattctt
    15841 gctagtccga tttatacgaa tcagaaggta gatataccag atgaggtaag cacgaacgcc
    15901 tttttaaaca cgaaagagct gacaatggtt tcaaaagaaa aaggagcgaa ccagcaatta
    15961 gtttttgaat ttgcgatcga tgatgaagat caaagcttgt taaaagaact ggctaatctt
    16021 aattatcgcg tagaagtaaa gacagctaaa ggggattatc aagcaatacg agccaaagtg
    16081 atcaaggtgt ctgatgatta ttttgcagtg aagctaacgc atgtaccaga agaatatatc
    16141 gccatgcgtt taaccattat tcctgaaaaa attgatccaa aagtggatat gcaagaacca
    16201 caagatctga tttattacgt gcacgaagat aaggttaaag atcaagtgaa agatgaagat
    16261 tatgaacaac acgcgatcaa ttacaaagtg aaaggataca agaaagaaca aaaagcagct
    16321 caaaaacaaa tggaatcttt ccaagcaacc attgatttga atgaagaatt agtgaagaag
    16381 ctaaaagatc aattgcctta tcaagtcgca gatgatcagg aaaatactga aaacaagatt
    16441 aatagctacc agcaagaaat tgaaacatcg aaaacgcaac tgaaagatca agaagagatc
    16501 attcaaaaac ttcaagaaaa aattgatcgt atgacaaaag aaaagattta gaaggacagg
    16561 tggagtgaaa aaatgaagcg aacaaacttg gctgacaaag atactttcgc tgagcagaaa
    16621 gtattgatta aagcctatct caaagttgta aaagctaaag aaatatgtcg agaagtgaat
    16681 ttatctaaca caacaatatc tgagttgaag aatggtcgaa agaatttaga tactttaaaa
    16741 ttcagatcat ttgaagctct atatgtagct gctaaaaaat attttacagc tgataacaaa
    16801 taaaaaacag gagtatttta tgtgaaaaaa gtatataaaa aatgcttatg aatcagctat
    16861 atcaccttaa ccatttgtta atgagatggg agaaaacatt aaccacgatt ctaacgcaaa
    16921 catcaagcac ataaaacatt taacactcct cctttctggc tgaagatttc aaccaaacag
    16981 gaagacagcg tatcaattat agcatacact ttttcttaaa ttcatttttc atatgacaaa
    17041 atatttcctt ctaatcgaca aagttagaag gattttttat tgtctaaata cacaaataac
    17101 cttaattttt aaagagcata aggaaacgtg atatcacttt ctcacgaaag gtgttaggta
    17161 ggctgtgcct accacgtttc taagaaagat aaatgatatc aaaattttgg atttgtgtgt
    17221 agatttgtgg gtaggatgaa agcgaatgtg ggcagtaaaa taagcgtttg tgtgtagcaa
    17281 aatcaaaaaa tgtgtaggga tatgtgtagc tacacatatc cctacacata ataaatttgg
    17341 aggaaaatat ggacttatta attagaaata ttgaagaaaa gtatattaat aaaattgaca
    17401 aacgttgtga agaacttacg gtcaagactg gcaaaaaatg gagtcgaaat caatacctaa
    17461 aagttttgat cgaaaatgat tttgatcacg ctcttctgaa ttataaaaaa gaccaatttg
    17521 atcggttgtt agaaaaattt gtggatattc aaacttataa tacgaaaact ttagaagaat
    17581 atattgcaac aaataatcaa ttaattggtt tgttaattga ataataaaaa tagtattgtg
    17641 aatttataaa aggaggaaat caattttggc aaaagcaaca agaaaacatg tatatcttta
    17701 cgatatgcat atcgaagtac ttgatgaaat aatttctact catgaagata tatctaatta
    17761 tagtgaagca gtcagacatt tgtgtatgat gtatgatcca aaaatcgaag aaaaaataaa
    17821 tccaattcaa attaaattga atgcaatggg aaaagaaatt tctattttaa ccgaaatggt
    17881 ttcagagttt gcggatcttc atttgaaaga tacaggaata ctaattggta caaattcaac
    17941 tgtttatcaa gaagcagaag aacgtgtgga aggaaaaatt aaatccaatc aaaccaaaaa
    18001 atattcttct aagttaaaga aaccagtgaa tgaatctgtt cataccagag tagatccatt
    18061 tgatccaacg aagattctaa aaagataaaa gtagaaggag aacgtgatga ataaatcgcc
    18121 tgcagttatt cttacttgtc aatttacctt gcccaataaa aaatcttttt caacatacat
    18181 tgattatatg acaagagaaa aagcactgga agaaatggaa cgacgtacgc ccgaagaaga
    18241 acaggaactg caacgaatca agaatgcttt agaggatttt tatattcctg aaggagaaac
    18301 tttttcggaa agtaagacgg ataaagaatt cagtgaaacg tcgaatgaag ctcaatcgtt
    18361 gatggaacat ggaatcaatt ttgacaagtt agaggaacaa gattttacca aatatctttc
    18421 ttatatgacc cgtcactatg cgttagaaca aaagaaagaa ctgacgcaaa gggaacaaaa
    18481 agaataccaa cggttgaacc aagcaatgaa aaaatatcaa acagagccac ataaaaaagc
    18541 ttcaggaaat cttcctggag ttttttctat ggcttcagac gaagtgaaag gaagtgatct
    18601 gaaggagatc aaacaaatct tccaaagagg acaacaaaaa ggctcaatca tttatcaaga
    18661 tgttatctct cacgacaatt cgtatcttga aaaaatgggc ttgtacgatc ccaaaacaga
    18721 taccttagat gaagaaggtt tgaaagcagc tggaagaaaa atgatggaga cattattcga
    18781 gaaagaacaa ttgaatgaaa caggttactg gatggcaacg attcaccgta acactaagca
    18841 tatccatatt cattttgcag ttgttgaaaa agaaaatacg cgtaaaccgt atactgagat
    18901 tgaaaatggt gtgagttata tcgtaccgaa ggggaagaga agacaagcaa cattagacgc
    18961 aatgaaaacc aattatgtac atgagttaga acgtttctct tatgaaaagg atcgaatgat
    19021 cggtatggag aaacgaaatt tattgacacg aaaaagtgat ttgcgtaaca ccttaacaaa
    19081 agtagtaaaa gaaccagcac gttacgatcg acacgccatg aacttattaa atgaagtata
    19141 taaaacccta cctgaaaaac gttcggaatg gaattatgga gatgaaaatc gaaccaagct
    19201 ttcaccattt acaaggggaa aacttgatga gttaaccaaa tatgttttaa caaaagaacc
    19261 tggatatcaa gaatatatac agatttcaaa agcattgaaa gaagaagcca aaagccttta
    19321 tggggaaagc aaaagagaaa caaagaatgc agaaaaaaac gccctctttg atttaaaaaa
    19381 gcgcacagga aatgcgattt taacagaact gaagagacag gatagtcaaa taaaaccatt
    19441 aatagaaagt gtcaatcaga tgcaatctat ttacaatgaa cgatcctttc gaacactcaa
    19501 acagttgcca acaaatgaat acaagtatag ttatcaagaa ttcgtaaagc gtaggaaaca
    19561 attccaacaa agagttgtca cgcagcgaag cttgcgtcaa ttaaatcgaa caattaatgc
    19621 acatgaagaa aaataccacg caagtaaagc atatgaagag caacagcgaa gaatccaaat
    19681 ggaacaagaa agacaatagg aggaagagaa atgaaacaat atttagatca atggaaagtc
    19741 atagaaggat cactaaaaga agaacgaatc gaacaacttc ctgattgttt agaaaaagag
    19801 catttgtttc agataagcga aatgttgaga aacgaacagt ttgatccaaa tcaattttta
    19861 gttgttgagt acccagctac tggtgtgtat tgttgtaatc atgtgaaggg tgaaaaatat
    19921 tttattattc aagaatacga aggtaagtta gcgccttatt atactacttg ggaaataaat
    19981 gaagagggaa tcaataattt cccatgtgaa agcattgaag agtctatttc tctcacggaa
    20041 tgttaaataa atagagacgt aaaagagtcc tgaacgtgat tgtccaggac tctttttcaa
    20101 tgaattgttt tccgccaatt cactgagtgg tgcgattcaa ttttattata tcaaaagaaa
    20161 gtgagattcc aaagtgaagg gaggaagaag aaatgactca gacaacgatt ggtgttgtgg
    20221 ttgaaaaaag cagggataat ctcgtttttg tgacagaaat tcaaacaggt agagcttttg
    20281 tagtcacaga caaagctgca aaagcgtatc aaaatggcaa tattttaacg ttaaatctga
    20341 ctacgaagat gttcgttgat gcagtagaaa attatccatt tgtttagaca aacgatattt
    20401 tacaaggagg aaacggacat ggcagaagaa aaagaaccac tagataatag tcgtttggga
    20461 aagtccaaaa gaaaattagt acggttgcaa aacgaattaa ataatcaatt tcaaaagcta
    20521 tttgatcacc aaaaattgac aaatgggcaa ccaatgaatg acaaacgaaa tggtcgttca
    20581 tggtttaacc agcaagaaaa aatagaaaat aaaatacaaa gtttgagaga agagatcaag
    20641 caacaagaaa aacaggtaga aaaacttgaa agacaagaag agctaaagga aatgggttat
    20701 aacaagtacg gtggcctgga catgacgatc gaaaatattc caagaatcaa agaagaaatc
    20761 gaacgctttg aaaaaggtga atcgactttt tcagctgcca cgatccgaaa ataccaacga
    20821 aaattggaaa cattagaaca gctgaaagaa aaatcagaga aaggaaaaga aaatcttctt
    20881 cctgaagtac aagcgatcat tgacagcgga cgagtgacgc aatggaaaaa gaatccaact
    20941 atctattttc tgaaaggcta tcgaaaagtc gctttagaac tcaatgaaaa cgggggattt
    21001 gaggaatcta aaaaatacca agccacgtcg gaagaagaaa aggcagctgt tcgtgaatta
    21061 cttgaagaag ctacacctac tgattctgtg aaggagaaag aaaccgatca tttctatcga
    21121 gtagaattca acgaaaatga tccagcaaaa gggttgaaaa gttatggtgg ggagatcgta
    21181 acactagaat tgatcgatca actcaaaaca ctggataacc aatacgccgc agaagcaggc
    21241 tattataaat tctattttga agaagtgaag aatggagaag tagtcgatca gatacgaatg
    21301 gatctagggg atggcgtcaa tagtaatcac cccatctatt tagagttagc tagattgtgc
    21361 gaacaatcac ctgaaaagga acaagaacaa acagtcaaaa atgaacctat ttcaaaagta
    21421 caaaacgaga ttaaagagac aaaaacaaaa caaaagggtt atcaaaaacg aacggcagaa
    21481 gagattaagc aagaagtcaa atcactttcc caacaggcgc ttgatgcggt gaaaaagcat
    21541 actcattcac ctgaagacgt caaagaactt ttgaatttta tgtctcgttt tccagaacga
    21601 tcctttagaa atcaattatt gatcgaggaa caatttccag gcgcaacggc ttgtttaggt
    21661 cgtgccgcgt tgaaaaaaga aggaatctat atcaaaaaag gagaacaagg gaacaagatc
    21721 tttgtcagaa aaacggtcaa aggattttat gagaaaggac gtggctttgt tcgagaaaca
    21781 gaggctacac cagaagataa aaaacgaatc gaaagtggtc aaatcgaagt gatcaaaaaa
    21841 ccttactaca cgattgaaaa agtgtttgat atcacacaaa cacaattgaa acctgaagac
    21901 tatccgaaaa tcttcccgaa ccgcgtcttt gattttcaat tggatcaaac agggcagaca
    21961 gaacttcaag ttgggattca agcggtagcc aaaaatattg gaattcaaat tcgagatatg
    22021 acagaaagtg aagtctatca aagagaatta ggacaagcac gtggggcgta cgttcgtaac
    22081 gaatttactg gcgaagaaga aatcgtcatg aatactcgaa atactcctac gcagcattta
    22141 gccacaagta ttcacgaatt agcccatgct agaatgcaca aattttcaga attagatact
    22201 gcaacaaaag aactacaggc agaaatgacc tcctatattg tgtgcaagca ttttgggatg
    22261 gacacctcgg agaaagctat tccttatatt gcgacatgga cgaagaatag tcagacgttg
    22321 gatgataagg aagctgcgga acgtgggaaa atcatgaatg atgtcagtcg tgtcgcaaat
    22381 gaattcattc aaaccatttc taatgaaatc aatcaataca gagaaagaga accagagatc
    22441 cagccagcag aagtgaagca ggagcataaa aatactgtag aaacggcaga acaaaagaat
    22501 attgtgcccg ttggctcact ttggatcgac aagaaagacg ggaaagtcct ggaagaagac
    22561 acgggccgaa cgttacattt gaaggatgcg gcgtttattg aagatccgca gcataatcaa
    22621 gtgattttaa aaggaaaaag ttatggaaaa gagatccaaa cggcgttacc ttcagaagaa
    22681 tttgcgctag gaaatccatt ccgattttca cttgagacag tgtcagaagt ggcaaaacga
    22741 aaaaatgaaa tctattatga acaacaattg tcacaaaaag aacgcgtata aaacaagcaa
    22801 gaaagactat ctgaaaaatg gatagtcttt tttcttttgg gaggtttgaa aatgacagag
    22861 aaaaaaatga gtctcattga tcgatgcaag caaatggata ttgtggattt tgcacgaaac
    22921 aatggtttag cggtggccaa taaaggacga gactatcgct tagaagacca tgattcattt
    22981 gtttttgatc gaagaaaaca acgtttttat tggaacagtc aaaatattag tggcgacatt
    23041 attgaattag ctaaactttt tttcatagac aaagagatcc aagaccctaa gcaacaattt
    23101 aaagcagcca ctgattttat tctaaagaat gaagacaaaa cagaacgagt ggaaaatctt
    23161 cattttgaga cagaaaagta caaagatcac cccgttgatt atcaaccact cacagaaaaa
    23221 ggaaggaatt atctgaagga agaacgaaaa ctgcccgatt ggttgattga ttatgcagaa
    23281 aaagaaggat tgattgccga actaaagcca aaacatgaac ggcaaaattt cttagttcga
    23341 gacgatcgtt tggatcatgc ggtcgccttt ttatggaaag atccacagac aaaagagaca
    23401 gtaggggcga gttatcaagg aacgttcatt gattatgaac gttttggtga gagaggcaca
    23461 tataagcata tagataaaaa ttcgacagcg aatcatggct ttaacctaaa gattggtgat
    23521 cctaaacagt taaaattttt tgagagtagt attgatttac ttagctacgc tgcactgaat
    23581 cgtgatcagt taaacgatac gtggttagtc tctatggaag ggttaaaaca ccatgtgatc
    23641 agtcattatt ttggtgaggc agtatctgaa ttgagaaaga aacaagcctt tcctcaatcc
    23701 atcgagattt gtgtggataa tgaccgtgca ggacatattt tctacgaaaa agaacaatta
    23761 atgggcgcag ttgatccatt tacgaatcag aaagtacgtt gtgaacgagg aatcgctaat
    23821 gattggcaag tgccaagaga atacaaagtc atttatgaag aagtcgcaaa agaagaaaag
    23881 gtcacaccag aagctattat ggccatacac aagacagaaa acaatctgca attaacgaac
    23941 caactggttt ccgctcacaa agtcaatgcg tcttttggtc agcagctttc tgttaacgat
    24001 tcaatagaag tcattagttt gaaagatatt tgtagagaag tcgcaaaaga attaaaagat
    24061 tgtgaacgct cgaatggtac gtatgatttt gatcgttttt atcaaggaaa aggggatatt
    24121 aattctcaga ttcttttctc ttataaagca gaacaatact acaaaggcta taaaaatcat
    24181 gaacatgaat ttgtacctga agtgaaaaag gattggaacg atcaattgaa gcatgagatc
    24241 catcaacagg aaattagaaa gcaaaaacgt gcgatgttgt ttcaacaagg cagacaacaa
    24301 gaaagggagt gagatcgtca cgatgaaaac tgtcatttta gcagaaaaac cgtcacaagc
    24361 gaaggcctat gccgatagct tttctaaagc cacccgaaaa gacgggtatt ttgaaatcca
    24421 agatcgattg ttttcgggtg aaactgtgat cacgtatggt tttggtcatt tagtcgagtt
    24481 agattcgccc gatatgtacg atgaaaattg gaaacaatgg tcattagagc atttgcctat
    24541 tttccctaca cattatcact atcatgttcc taaagataag aaaaagcaat tcaacgtggt
    24601 gaaacagcaa cttcaatcgg ccgacaccat tattattgcg acagatagcg atcgtgaagg
    24661 ggaactgatc gcttggacga tcattcaaca agcaggcgct gatcaaggaa aaacctttaa
    24721 aagactttgg atcaactcct tagaaaaaga agccatttat cagggattcc agcaattacg
    24781 agacgcagaa gagacctatc ccaaatttga agaagcacaa gcacgccaga tcgctgattg
    24841 gttgatcggc atgaacggca gcccactgta tagtttactc ttgcagcaaa agggcatacc
    24901 aggaagtttt tccttaggca gagtccaaac cccaacgctt tacatgattt atcagctgca
    24961 ggaaaagatt agaaacttcc aaaaagagcc ttattttgaa gggaaagccc aagtaaccgc
    25021 tcaaaatggc atatttgatg cgaagcttga tcccaatgaa acccaagcga cgcaagaagc
    25081 atttgaagcc tatttaaaag aaaaaggtgt tcaagtagga aagcagccag gcacgattct
    25141 tgaagtcgaa accgagaaaa aaagcgcggc aagtccacgt cttttttctc tatccagcct
    25201 tcagtctaaa atgaatcagc tcatgaaagc tagtgccaaa gatactttgg aagccatgca
    25261 agggttgtat gaagggaaat atctaagcta tccacgaaca gacacccctt atattacaga
    25321 aggagagcat gcgtatctgt tggatcattt agatgaatac aagcattttt taaaggctga
    25381 atcattccct actcctattc atacaccgaa tagtcggtat gtgaataata agaaagtcca
    25441 ggaacattac gcgatcattc ctacaaaaac agttatgaca gaagcaacgt atgaaaagct
    25501 gacaccttta caacaagcga tttatgatca agttttaaag acgacggtgg ccatgtttgc
    25561 ggaaaaatat acctatgaag aaactacgat tttgacacaa gtccagcagc ttcagctaaa
    25621 ggcagtcgga aaagtaccag tagatctagg ttggaaaaag ctatttggca aagagagcga
    25681 cagaaaagag aaggaagaag aaccgctact tcctaaagta acaaaaggag aaacggtgac
    25741 ggtagatcta caaatctttg aaaaagaaac caagccacca caaccttaca cggaaggtac
    25801 actaatcacc gccatgaaga cagctggtaa aaccgtggat agtgaagaag cacaatccat
    25861 attgaaagaa gtggagggaa ttggtacaga agcgacacga gcaaatatta ttgaaacctt
    25921 gaagcaaaag gaatatatca aggtagagaa aaataaactt gtggtgacga ataaaggaat
    25981 tttgctttgt caggctgttg aaaaagaacc gctgttaaca agtgccgaaa tgaccgccaa
    26041 atgggaaagc tatcttacaa agattggtga gcaaaaaggg acacaagcta cttttctagc
    26101 aaatatccaa aaatttgttt cccacttgtt ggaagtcgtc ccaggacaaa tccaagccac
    26161 tgattttgga gcaacgttac aagaggtgaa agtagccagt gaaaagcaag aagcggcgcg
    26221 tcatttagga tcttgtccaa agtgccagga aaaagaagtc atactttatc agaaggtggc
    26281 gacttgcacg tctgaagcct gtgattttaa attgtgggta acgattgcca aaaagaaact
    26341 cacagcgaca caactgaaag agatcattca gaacggccga acaagtcaac cagtcagagg
    26401 actaaaaggt cagaaaggtt cgtttgaagc aacgatcgtt ttaaaagaag actttacaac
    26461 aggatttgag ttttctgaaa agaagaagac cacctacaaa agaaaagtga gacgtaaaac
    26521 gagttagaat tgacggaggg aaaaacgatg agaaatacaa cgcataaaga acaagaaatc
    26581 gaaaatcaaa aagccaaaat acgttcagag caggagaaaa tgcgtgccag caaacgaaga
    26641 ctacagcaat tgaagaatca acaacggatt caaaaagaac ttgaattaga tcgataaatc
    26701 ctcctgttgt taatattaaa ctaacatgtt atagttatgt tagtttaata ttaacattcg
    26761 agagggaaag cgatgaaaga taaagtcaag tgcatttttg agaaatacaa tggtaatcta
    26821 tccttgaagg atgctagaaa tgaagggtta tcacctatga cgttagatcg tttagccgaa
    26881 aaggacaaaa tagaaaaaat agcatcaggt ttttatacct taaaagatag ttttgtggat
    26941 gaattatatc tcgctcaaag tatctatact agaggaatct tctctcacga gacagcgctt
    27001 gatctttttc aagtaggaac atatatccct aaaaaaatta atatgatgtt tcctaaagga
    27061 tacaatgttt cgaaagagaa tttagatcag tatgccattc aaccgcatta cacgaataaa
    27121 gaaaactttg aactgggaat cacagaggca gaaagctttt acggcaataa aattttcgtc
    27181 tatgatttag agcgaacgtt atgtgatatg tggaatccca gatataaagc aagcgtagaa
    27241 gtaaaacaag aagcattaaa agaatatatg acaagtacca gcagaaatac aaataagcta
    27301 agaagataca tgaatatttt aaaatcacca aaagaaatga ctttgtatat gttgccactt
    27361 tattgagagg agtaaaggtg aatgacaccg acacaattta aagcgaaaac aagaaatgtt
    27421 gcagctgaga aacatttaaa cccacaaatt gttcaaagat attatatgat ggaaaaatta
    27481 ttagaatgta ttgttgaatc gccttatcat gatgattttg tattaaaagg gggatttctg
    27541 atcggatcaa aatacgggtt agaaaaccgt tcaaccgttg atattgatac gacttatcga
    27601 aattcgaaac taacaaaaga aaaattagaa actgtttttt ctgaattaac agctgctcct
    27661 acaaaagatg gcattcggtt tgaattaaag ggcttaacag agacgaggga agccgattat
    27721 tacccaggtt ttcaagcaaa gtttattgct tttcttgaaa atgcacgtgt accttttaaa
    27781 ttagatatta cttcaggtga ttctattatt ccagcagcat tgatatatga acataaattg
    27841 atgtttgaag ataaaacaat tcatattcca gcttatccta cggaacaaat tttagctgaa
    27901 aaattacatg ccacgtttag tttcggaata gataattcac gtatgaaaga tttctatgat
    27961 ctgtatatga tccctaaact agaacgtatc gatgagataa atttgtataa gagtgttcaa
    28021 ggaacattta aggaaagaaa taatcaatca gtgtttaaga atttgtataa tcaggaaatg
    28081 cctcaattga tgaagaatga gggattgagg gagaaatgga aaagatatca gcaagaaaac
    28141 tattttgcca aagatatttc tttcgaatct accttaactt ctgttgattc gctgatgcaa
    28201 tcaattgtcg agcaagagga agtagaacga aaacaaaatt ttaagagatc acaacaaatg
    28261 aatcaagaaa tggagagata aagatgatca ataatgtaac tttagtaggt cgattaacca
    28321 aagatcccga tttacgttac accgcaagtg gcactgcagt gaccactttt accttagccg
    28381 ttaaccgtaa ttttactaat caaagtggca atcgagaagc tgactttatc aattgtgtga
    28441 tttggcgcaa accagctgaa acgatagcga cattagctaa aaaaggcagc ttgatcggcg
    28501 tcgtcggacg gattcaaacg cggacttatg acaaccagca aggacagcgt gtctatgtga
    28561 ctgaagttgt ggccgataat ttccaactat tagaaagtaa agcagcgaca gaaagccgta
    28621 tgcacgctga tcaatcgtcc agcagtccaa gccctaccac ctttgaacaa cgggatacag
    28681 cgacgcctaa caacaatgga ttgaacgctt cacagaatcc atttggtgga caatcaatcg
    28741 atattagcga tgatgatcta ccgttctaag tagcattttc tattggttgc atttgctacg
    28801 gtttatgtta taatttagac aacaaaaaga attttcaatc tataaccaaa aaccactagc
    28861 tggcgggcta gtggctttct tttacgtttc tattataaga attaacg
//
LOCUS       DAGCYL010000017        28417 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.017, whole genome shotgun sequence.
ACCESSION   DAGCYL010000017 DAGCYL010000000
VERSION     DAGCYL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.017"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="MB216_000560"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="MB216_000560"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBT4525418.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="MB216_000561"
     CDS             complement(937..1518)
                     /locus_tag="MB216_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4525419.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="MB216_000562"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="MB216_000562"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBT4525420.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="MB216_000563"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="MB216_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBT4525421.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="MB216_000564"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="MB216_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBT4525422.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="MB216_000565"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="MB216_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBT4525423.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="MB216_000566"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="MB216_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBT4525424.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="MB216_000567"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="MB216_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBT4525425.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="MB216_000568"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="MB216_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBT4525426.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="MB216_000569"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="MB216_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBT4525427.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="MB216_000570"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="MB216_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBT4525428.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="MB216_000571"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="MB216_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBT4525429.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="MB216_000572"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="MB216_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBT4525430.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="MB216_000573"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="MB216_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBT4525431.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="MB216_000574"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="MB216_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBT4525432.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="MB216_000575"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="MB216_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBT4525433.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="MB216_000576"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="MB216_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBT4525434.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="MB216_000577"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="MB216_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBT4525435.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="MB216_000578"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="MB216_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBT4525436.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="MB216_000579"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="MB216_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBT4525437.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="MB216_000580"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="MB216_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBT4525438.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="MB216_000581"
     CDS             13972..14157
                     /locus_tag="MB216_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBT4525439.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="MB216_000582"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="MB216_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBT4525440.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="MB216_000583"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="MB216_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBT4525441.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="MB216_000584"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="MB216_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBT4525442.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="MB216_000585"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="MB216_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBT4525443.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="MB216_000586"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="MB216_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBT4525444.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="MB216_000587"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="MB216_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBT4525445.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="MB216_000588"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="MB216_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBT4525446.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="MB216_000589"
     CDS             18232..18879
                     /locus_tag="MB216_000589"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBT4525447.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="MB216_000590"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="MB216_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBT4525448.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="MB216_000591"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="MB216_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBT4525449.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="MB216_000592"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="MB216_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBT4525450.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="MB216_000593"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="MB216_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBT4525451.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="MB216_000594"
     CDS             20317..21255
                     /locus_tag="MB216_000594"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBT4525452.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="MB216_000595"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="MB216_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBT4525453.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21934..22773
                     /locus_tag="MB216_000596"
     CDS             21934..22773
                     /locus_tag="MB216_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBT4525454.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22749..23618
                     /locus_tag="MB216_000597"
     CDS             22749..23618
                     /locus_tag="MB216_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBT4525455.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23608..24405
                     /locus_tag="MB216_000598"
     CDS             23608..24405
                     /locus_tag="MB216_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBT4525456.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24608..25354
                     /gene="truA"
                     /locus_tag="MB216_000599"
     CDS             24608..25354
                     /gene="truA"
                     /locus_tag="MB216_000599"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBT4525457.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25687..28356)
                     /locus_tag="MB216_000600"
     CDS             complement(25687..28356)
                     /locus_tag="MB216_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBT4525458.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         9217 a         5288 c         6041 g         7871 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aacattttaa gcgagaaata attgaaaatt ttgtcttttt ttgatagtgt
    21901 tagtaggaac tttaggatga agaaggagta atcatggagc caattattga attagaaaag
    21961 ataaattata aataccagcc agatgatctt cgccccgcat tgaaagatgt ctcctttaca
    22021 attgataaag gtgagtggat cgctattatt ggacacaatg gttcaggaaa atcgacactt
    22081 gccaaaacga tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt gggaaatcaa
    22141 atattggatg aagaaaatat ttggacgatc cgacagatgg ttggaatgat ttttcaaaat
    22201 ccagacaatc agtttgttgg ttcgacagta gaagatgacg ttgcttttgg tcttgaaaat
    22261 caagggattc ccagagaaga aatgctagtg cgtgtgaaag atgcacttga gaaagttaga
    22321 atggctgagt ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa acaacgtgtt
    22381 gccattgccg gcgtggttgc cttacgacct gatatcatta ttttagatga agcaaccagt
    22441 atgctagatc cagaaggtcg tgaagaagtg atctctacga tcaagaagat caaagaagaa
    22501 agtcaattga ccgttatatc tatcactcat gatattgatg aagcagctaa tgccaatcgg
    22561 atactagtga tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga aattttttct
    22621 gctggtcctg aactgatcga cttaggtcta gatctaccat ttcctgaaaa actgaagagc
    22681 gcactgaaag aacgtggggt tgatgtgccc agtgaatata tgacagaaga aaggatggtg
    22741 gattggttat ggacatccgt tttgaacaag tagacttcac ttatcagccg aatacaccat
    22801 ttgaacaaag agccttattt gacatcaata tgacgatcaa agaaaacagt tatactgcat
    22861 tagtagggca taccggaagc gggaaatcaa ccttacttca gcatttgaat gcgcttgtta
    22921 agccaacaag cggtacagtc catattggag aacgagatat tcagccggat acggataata
    22981 agaatttaaa gcccatccga aaaaaagttg gcatcgtctt tcagtttcca gaagcacagc
    23041 tattcgaaga aacggtagca aaagacattg ctttcggtcc taaaaacttt ggtgtcagcg
    23101 aagaagaagc actagtccta gcaaaagaaa cattagaaca agttgggctg gatgaaagct
    23161 atttggaacg ttcgccattt gaactttcag gagggcaaat gcgtagagta gcaatcgctg
    23221 gtgtgcttgc catgaggccg gaagtacttg tattggatga accaacggca ggacttgatc
    23281 cccagggcag aaaagaaatg atggagatgt tttggcgtct tcataaggaa cagcagatca
    23341 ctattgtatt agttacgcat ttaatggatg atgtagccaa ttttgcagat tatgtctatg
    23401 tgttggaaaa gggacgaatc gttaatagcg gggagccgca agaggtcttt caaaacattg
    23461 aatggctaaa agaaaaacaa ctaggcgtac ctactgccac cgagtttgca gaagaattaa
    23521 tggctaaagg gatgaacttt gctaccttgc ctttgacagc tgaagaatta gcagacgcta
    23581 tcgtccatta tgctggagga aatgcccatg atgaataaat tgatatttgg tcgttatatg
    23641 cctggagatt ccttgatcca tcgattggat ccccgagcta aattattagc cagtttttat
    23701 tttatcggaa tcattttttt agcaaataat tgggaaacgt accttttgtt ggcatttttc
    23761 acattacttt ctgtcttctt gtcaaaagta gatttgaaat tttttatccg aggcattcgc
    23821 ccattgatct ggctaatcct cttcactgta gctttgcagg tcttgtttac aagtggcgga
    23881 gaaacttatt ggagttgggg tgttttccat atcactgaat ttggtgtgat gaacggcctc
    23941 tttattttct gtcgattcgt gttgatcatt tttatgtcga ctctactcac gcttactaca
    24001 ccgcctttgg aattatcgga tgcgattgag tacttgcttc gtccattgaa agccgtacgg
    24061 ttcccagtcc acgaaatttc attgatgctg tcgattgctc ttcgttttgt gcctacattg
    24121 atggacgaaa cagaaaagat catgaacgct cagcgcgctc gcggtgttga ttttggtgaa
    24181 ggaaacctag ttcagaaaat gaaagcagtc attcctttgc tgattccttt atttgtcagc
    24241 agcttcaacc gtgcggaaga tcttgcgact gctatggaag caagaggcta caaaggtggg
    24301 gaaggaagaa cgaaatatcg tgtattgcat tggcataata gagacacgat ggttatcatt
    24361 gcttttgtat tattaactgt gctactgatc tttttaagag gataaacgtt cagtgggccg
    24421 ctatctattc ttttcttgct gatctgtttg caagaaagga tgcaagcagt gtatgacaag
    24481 aaatattagg gtctgtggca gactaaggtc acaggcctct ttcttttatc tgaaaagaaa
    24541 gaacgaaaaa attttataga aaaatcatta ttccagataa ccttttagga acaaaggaga
    24601 gaaaaacatg gtacgttaca aagcaatcat tgcgtacgac ggaacaaatt ttaatggatt
    24661 ccaaaaacag ccaaatgggc gtactgttca agaagaagta gaaaaaacat tacaaaaaat
    24721 ggcaaatgga aaagaaatca cggtctttgg ttcgggaagg acagatgccg gagtacatgc
    24781 gatcgggcaa gtaatccatt ttgattatcc agaggagcgt ccgttagaac gaatgcgttt
    24841 cgcgctagat acccagtcac cagaggatat tgctgtaaga caagtagaaa ttgtttcaga
    24901 agacttccac gcaagatatt tggtcaaaga aaagacgtat caatttcgag tggatatagg
    24961 gaaaccacgc agtccttttc gacgccatta tgcaagttat tttccttacc cacttgattt
    25021 ggaaaaaatt cagcgggcgc ttcctgattt attagggact cacgatttca cctctttttg
    25081 tgcatcagga agctcgatag aggataaaat acgtacgatc tacgaggcta agatggaggt
    25141 aaatgaaaca aaagacgaat tgttgtttac atttagggga aatggcttct tgtataagat
    25201 gatacggatc ttagtaggaa cgcttttgaa aattggcaat ggccgattgc ctgaagatag
    25261 tatcccagat attattgcga aaaaagatcg taatgcagca ggacctacag cacaccctga
    25321 aggactttat ttgtatgaag tgaaatatga ataagaggtt atgatagaag tggtcagctt
    25381 caagaaataa gaaggtattt acgaaaatta cttctcaaaa ttttgcaaaa tttcggctta
    25441 ttttcgaaga agctacttct ggaacaccgt ttaagaaaga ggtcgtgagc ctgtcattta
    25501 actctgagtc acaaagagca atttcaaaaa acagcttctc atctttgatg aatatcgtga
    25561 cttgtggacg aggcgttgat aagtgaatca tctttttgtt acctattttt tctgtaacca
    25621 aaaaacgatg gaacgttgtt ttgattgccc ttggtgcgga cagtctgaac aggttccatc
    25681 gtttctttat tttctacgtc ttcttctgtg tgtgtttcgt ggttgttctt ctattgctgg
    25741 atcaatcgtg ccataaaagg ggcgattgcg gtcaaggtac cagacataaa gaataaacaa
    25801 tgcaatacat aggacaaaaa ggattgcacc tatcgtaaat ccttcaggca agtaactgaa
    25861 agtgacagta tgtttacctg ctggaacttt tatcatcaag aatgcgtctt tgaaattgct
    25921 gaccttgact ggtttcccat caattttagc tttccatcct ttgtcataag gaatagttgt
    25981 caccaacatg gaatcttcag cggtatttac agtcccagaa gcttttctgc cagtggcttc
    26041 catttcgaca cctttttctt tgatctgatt cattgcggct tgataggcta caacatctag
    26101 accaacgact ttaggctcca tgaaacttat ttctttcgtc ccataaaaac ttgcagtaaa
    26161 tgtcactgtt gtctcttgag gataatagcc aagatcatag tattgaccag aaatgttgat
    26221 ttgcgttttt cgcgatgtac cattgacagt gatcgtcgca ctagaacttt ctaattgcgc
    26281 aaaattggtt gggaacaaac ttaagtaagc ttgtgtattt gcaggcacat ggacttccca
    26341 agtgagctct tttggctggt tatctgctgt ttctgagtaa gtgacgccag tcgttgtctt
    26401 tgtgatcgtc gtattcaact gattcttcaa agtaggtgta tagaatgtaa aatactgttc
    26461 atttagacca gacaatgtat tgatcaagtt tgtctgactg gtcaaattat ctgtcactgg
    26521 ctgctcaaca tcattaacgg acaacggtgc agtaaatgct agtggcaatg cattgacatt
    26581 ctgatacagt gtatagtcgc cgctttgacc agcttggcgg aagccatatt tactgaacgt
    26641 gctttcgttt ttagaaatgt tatatttgat tcctgtaaac ccatccatca gcaaagtatt
    26701 attgttataa cggatattca aatttgtccc tcttgaacgg aaacctaatt tatccaggta
    26761 agaggaagaa ttacgatttc ttatagaaga aaacaaactg ataccactgt aaccatagtt
    26821 gatcgaatcg tttgcagata ctggatccag attttccaag cggtaaaacg agtcggaatc
    26881 tttttttgtt tcatttacaa gagctttcaa gtcaggataa ggatcagtat ataaactgcg
    26941 ggaagcataa ttccaatcat caagaatgcc atggatcata ctgttcgtat tgaccagtgc
    27001 ttcagcagac atgatcaata ataacaatat actcagatga ttgaatgaaa gttttttatg
    27061 ctgataaaat cccacagcaa aagcataaat ccctaagaaa accactgtca atacaaagga
    27121 agtcatcgtc acataggtgt agcttccttc aggtttgatg ccccatgcca ccgcaaagag
    27181 agcagctacg accaaaatga ttcctaacaa cagcccacca tcgtcttgtt ctatcttttc
    27241 ccatcctttt cctgcaagca ggagcactaa gaaagagaac agataggcat aacggaataa
    27301 aaacatattc ggtgcatgca taccatgcca gaacaaattc aatggaacaa gataaaaact
    27361 tgcgataagt atggcgaaaa gactgccgta caaaaattta tttttcaacg gtactttccg
    27421 actggcaaaa taaaacagac agaggactaa tggtagcaga cctgcgtaaa cgaacggaat
    27481 cgatccgtat tttgtcgtgt cataaacacc aatcatattt ttcatgatta gatccagata
    27541 agaagtcgct tctgttttca acgtagtgat ctgactcagt gcctcgccat ttgaccgaag
    27601 atccagcact gctggcaata caatgatcat tgaagcgccg cctgccagta aagaagtgat
    27661 accataagga acaatcgttt ttttatattc tgaccagttt gtcaatagac gggcgataaa
    27721 atataagaaa gaaaatacgc caatcataaa acccatataa aaactggata aaaacaagag
    27781 taaataactg ataaataaaa gtttcggccg tttaagatcc ataacacgat gaatccccaa
    27841 aatcactaat ggaagataaa caaatgcatc cagccacatg atgatctccg aatgtgcagt
    27901 gataaatgag atcaatgcgt aactgacgct aagtgcaaca tgcccccagc gaggaatctt
    27961 gaacgtacgt gcaagaaacc aaaaagccaa tccggcacag ccgaccttaa gcaaagtcag
    28021 aacatacagt gcatctggca tcatctggtt tggaaaaaag aacaccagtg gcgtgaagat
    28081 cccgcctaga tagtaagaaa ttaaagataa ataattcaat cctagagaag cattccatgt
    28141 ataaaaaata ctttgctttc caagaagaac atttcggaag ctggcatgaa aattagaaaa
    28201 ctgtgaaaag gcatcacttg ccaaaatact tctgctgctt ccaggataga ttccgatact
    28261 tagataaaca agtgctaaaa tcaaaaacgg aatcaggaaa ctagctgctg cgtagcgtcc
    28321 gttttctcta aagaatgact ttaaattttt tttcatattt attttccacc aaatttctat
    28381 gaagttgtat tcagtttatc atatccttgt ttttctc
//
LOCUS       DAGCYL010000018        27946 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.018, whole genome shotgun sequence.
ACCESSION   DAGCYL010000018 DAGCYL010000000
VERSION     DAGCYL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.018"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            156..854
                     /locus_tag="MB216_000601"
     CDS             156..854
                     /locus_tag="MB216_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HBT4525459.1"
                     /translation="MQELVILKNKEAVTTSIQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFSEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNTKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            1032..1373
                     /locus_tag="MB216_000602"
     CDS             1032..1373
                     /locus_tag="MB216_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525460.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            1366..2037
                     /locus_tag="MB216_000603"
     CDS             1366..2037
                     /locus_tag="MB216_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBT4525461.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            2043..2729
                     /locus_tag="MB216_000604"
     CDS             2043..2729
                     /locus_tag="MB216_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525462.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            2736..3587
                     /locus_tag="MB216_000605"
     CDS             2736..3587
                     /locus_tag="MB216_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HBT4525463.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            3599..3868
                     /locus_tag="MB216_000606"
     CDS             3599..3868
                     /locus_tag="MB216_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525464.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            3873..4037
                     /locus_tag="MB216_000607"
     CDS             3873..4037
                     /locus_tag="MB216_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525465.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGERRDG"
     gene            4030..4350
                     /locus_tag="MB216_000608"
     CDS             4030..4350
                     /locus_tag="MB216_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525466.1"
                     /translation="MDKKAAWRKLMLLIQDENWQEDEAVVAEVQRLEKIANGRIRKKP
                     DKRKLRKGKIIVVLYEGNILMQGTARELSAETEYTSGTIRTYAWRNHVDKKGHEYKYL
                     EGSK"
     gene            4347..4736
                     /locus_tag="MB216_000609"
     CDS             4347..4736
                     /locus_tag="MB216_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525467.1"
                     /translation="MIPKFRAWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVGIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRV
                     FKHKKMDYEYEVIGNIYENSELLEEQR"
     gene            4733..5326
                     /locus_tag="MB216_000610"
     CDS             4733..5326
                     /locus_tag="MB216_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBT4525468.1"
                     /translation="MNKQETIKKLESIKAIGNDAIAACYNESMNAGITLMKKIDEPQK
                     HVMPKFFDKWAKQVLEKRDKFYAISLIARAGWGYGVDFELSENGLSSENKELLYWLVD
                     KCSDNYPNKKKAIEALLYGYEVEKEPLYHVKLKIPGVTYYLIQTFLMPGDTHMCFSAA
                     TERYGAKWKNTFTESEIKAIDERYWPFAVPVEEEAEG"
     gene            5323..5700
                     /locus_tag="MB216_000611"
     CDS             5323..5700
                     /locus_tag="MB216_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525469.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQQIVFEWLKLTAPTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKEQFEVLAAFAQWGLEQEEAE"
     gene            5697..5900
                     /locus_tag="MB216_000612"
     CDS             5697..5900
                     /locus_tag="MB216_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBT4525470.1"
                     /translation="MNNRHRRITKLRKQELNVLKAKFEKEYGVSAEEAYKVASQCVAD
                     ASNAIRKFGISILSDDRKWEDIE"
     gene            5897..6106
                     /locus_tag="MB216_000613"
     CDS             5897..6106
                     /locus_tag="MB216_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525471.1"
                     /translation="MKVYVVRFGNKFYRSDERSIGNNVLFLVDFLQNARWFDNLEEAN
                     QVSRRLGGLTQVYELVTIDHEGVER"
     gene            6103..6396
                     /locus_tag="MB216_000614"
     CDS             6103..6396
                     /locus_tag="MB216_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525472.1"
                     /translation="MKLKDGFYASSHGIGGLMLGMPTKNPKTRKKPKFKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKRLAKSKENLAVVRLVDMEVIKS"
     gene            6426..6866
                     /locus_tag="MB216_000615"
     CDS             6426..6866
                     /locus_tag="MB216_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4525473.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            6941..7408
                     /locus_tag="MB216_000616"
     CDS             6941..7408
                     /locus_tag="MB216_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HBT4525474.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGMSIYVTAQRNNTSEESVKAESNKIIIQFASSLELVAFK"
     gene            complement(7818..8171)
                     /locus_tag="MB216_000617"
     CDS             complement(7818..8171)
                     /locus_tag="MB216_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525475.1"
                     /translation="MAKIDDYRSDMIEYIELFAKNSNNNIIIQTGGATICGTPIDFDA
                     EVKVNPLIDAMMDSFAEFRSKKIDDIEKDDEQSLVVKSIFLKDVTIIGERTTNIPFLV
                     VFADQISAISLGNLE"
     gene            9102..9446
                     /locus_tag="MB216_000618"
     CDS             9102..9446
                     /locus_tag="MB216_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBT4525476.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            9451..9732
                     /locus_tag="MB216_000619"
     CDS             9451..9732
                     /locus_tag="MB216_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525477.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNIFGYSIEEINYYIDFAEAFIPMVR"
     gene            9835..10149
                     /locus_tag="MB216_000620"
     CDS             9835..10149
                     /locus_tag="MB216_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBT4525478.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            10127..11821
                     /locus_tag="MB216_000621"
     CDS             10127..11821
                     /locus_tag="MB216_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBT4525479.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPGLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENAGTGFTPLVF"
     gene            11841..13019
                     /locus_tag="MB216_000622"
     CDS             11841..13019
                     /locus_tag="MB216_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBT4525480.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRAQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYKLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            12982..13668
                     /locus_tag="MB216_000623"
     CDS             12982..13668
                     /locus_tag="MB216_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBT4525481.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKREEKIAAFTAAL
                     NKTFGQGDVK"
     gene            13668..14828
                     /locus_tag="MB216_000624"
     CDS             13668..14828
                     /locus_tag="MB216_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBT4525482.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTRA"
     gene            14838..15713
                     /locus_tag="MB216_000625"
     CDS             14838..15713
                     /locus_tag="MB216_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525483.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            15710..16021
                     /locus_tag="MB216_000626"
     CDS             15710..16021
                     /locus_tag="MB216_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525484.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            16011..16364
                     /locus_tag="MB216_000627"
     CDS             16011..16364
                     /locus_tag="MB216_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBT4525485.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            16354..16755
                     /locus_tag="MB216_000628"
     CDS             16354..16755
                     /locus_tag="MB216_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525486.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            16748..17152
                     /locus_tag="MB216_000629"
     CDS             16748..17152
                     /locus_tag="MB216_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525487.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            17164..17772
                     /locus_tag="MB216_000630"
     CDS             17164..17772
                     /locus_tag="MB216_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4525488.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            17792..18154
                     /locus_tag="MB216_000631"
     CDS             17792..18154
                     /locus_tag="MB216_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525489.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            18157..18339
                     /locus_tag="MB216_000632"
     CDS             18157..18339
                     /locus_tag="MB216_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525490.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            18356..21775
                     /locus_tag="MB216_000633"
     CDS             18356..21775
                     /locus_tag="MB216_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBT4525491.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFIDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAVATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            21826..22563
                     /locus_tag="MB216_000634"
     CDS             21826..22563
                     /locus_tag="MB216_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4525492.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFTCTGLSKIQIYRNGLR"
     gene            22573..24864
                     /locus_tag="MB216_000635"
     CDS             22573..24864
                     /locus_tag="MB216_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4525493.1"
                     /translation="MIDNLLTIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNVGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            24888..27131
                     /locus_tag="MB216_000636"
     CDS             24888..27131
                     /locus_tag="MB216_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525494.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPYLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAETKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGNYDYSGNPNLLSKLSYDLIE
                     NQNTSAGTLSKGENSFKYNKISAETEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGV
                     DYSPADGKKILIRYRFVDNGTGKIVLDLPINSNSITQEWKEFSITGTTPTFGPQAYHP
                     WIQFRAQDGILGEIEMSYDIKIEEGPTATPFQPNLLAEPYNMCREYPNENIVNPTVKF
                     PIKTSNNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQLQHQATLLP
                     VEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEKGNTRTPNIS
                     EYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQ
                     LTASINEVDTTAKDARTKANANATAIDELDNKIDERINDAATTAFYGAQGEVSNKTKV
                     AEYGVGTGYTTTAVGETHFERQSNGYVKCLKAGKYSISAQIRVQLGGKYASWLYTDLY
                     KDGTSVDNLVSYGVDALQNRFAASGNIVTDLEVNDVLNMRTEIGVADGILQFTSCRTL
                     VLTRLSD"
     gene            27209..27451
                     /locus_tag="MB216_000637"
     CDS             27209..27451
                     /locus_tag="MB216_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBT4525495.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQVYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            27467..27664
                     /locus_tag="MB216_000638"
     CDS             27467..27664
                     /locus_tag="MB216_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBT4525496.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMIVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            27675..>27946
                     /locus_tag="MB216_000639"
     CDS             27675..>27946
                     /locus_tag="MB216_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBT4525497.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVG"
BASE COUNT        10004 a         4167 c         5878 g         7897 t
ORIGIN      
        1 aatgttgaaa atactagaga cgtaataggc aaaataaaat ctttaatggt tttaacaatg
       61 ttattccagt caaattgttt aataaagtca ataagactca atttatacca ccaccatttt
      121 ttatctacat tatatcaaag aggagagaag aaataatgca agaattagta attttgaaaa
      181 ataaagaagc tgtgactacg agcatacaag tggcagaaag ctttgagaaa aaacatcaac
      241 atgttttaag agatattgat gcactaaaaa aagatgtgtc caattttgga cagatgtttt
      301 ctgaaggtaa tgagccagat tcctacggaa gaaatcgacg agttttcttc attagcagag
      361 atggattttt cttgttagct atgggattta caggaaagaa agctatttac ttcaaacaaa
      421 aatacattga agcattcaac gaaatggaag atgttattcg caagaatact gttcctcaaa
      481 caattgaaga catgatgatc taccaactag aagaaatgaa agatgttaaa aaagatgtct
      541 ccatgcttaa agatactatg cgaattagcg gacaacaaga gtttgaaatt aagcaaaaag
      601 gaaatacgaa agttatggaa gttctagggg gaaaagaaag ccgagcttat gaagaaatca
      661 gcaaaaaagt attctcaaaa ttttggtctg aatttaaacg taccttttca atcccaagat
      721 atggcgagtt acctcgtaag agattcgatg atgctgtttc atttattgaa atgtggttac
      781 cagaaactgc gattcgcatg gaaatcgatc aactgaacag acaacaaaga ctttttggtg
      841 atgaaaatga atagagctga agcgctaaga atcgggacgg taattgctaa tcgctggtgg
      901 agacacaata aaccaatcat cctaagccaa caacatattg acaagcaaaa agcatggcaa
      961 caaataaaaa agtgactccg ccggcaagca aagagtcaca aagaaaacac atcataagga
     1021 gattttagca tatggaaaaa gaactttcca ctctagatca atatttgact gatcctagtt
     1081 ggggcaaatc gaatatcaag gaaacaaata atcgaaaaat cagacgaaat cttttgacgg
     1141 atgaagaact agcatgtgat caagacgatt tgggaaattt tgtgagtatt tgggatcatg
     1201 tctatcttat ccatttatcg aggaagtcca aaaaacctga atatatctat gtcatcgaag
     1261 atggcttgat tgatgcgcta gaagagtatg acagagataa cttgattgat atctcttatt
     1321 acggatcagg taagaaatac attgctgaaa tggaggcaga atttgatgag tgaaaacaaa
     1381 gggacaacga cttttgaaaa actttttagt cgcaagttaa ataaaatact caagaaaaaa
     1441 ggaaattttg attatttatc ttgggctcac gcatgggaga ttatgaaaaa gaatgatcca
     1501 caggcaacgg taactattaa tgagtataaa cactacagag ttgtttctgg aactcatcaa
     1561 gactttcttg ttgaggaata taaacctttt cttatggatg aaactgggac ttatgtatct
     1621 gtctcagtaa cggttaaagg acacacggaa accgaattat ttcctgtttt agattatcga
     1681 aaccaaccag ttgttaaacc aaatgcaatg caaatcaata actcattgaa gcgatgcttt
     1741 gtgaaagcat tggctctaca cggactggga ttatatgtat ttcaagggga agatattcca
     1801 acaccaccta gaatcgatac gaagaaatta aacatgctag agacgattct agaagctttc
     1861 aatgagcaga tgggtaaaga tatgaccaaa accttaatcg aatatgttaa tgagcagaca
     1921 gataaattag ggctcttagc tgataacgtt gaaactattg aacagttaag ctatgagcaa
     1981 tgtgccttga tggagcgagc aatagcagct aagagaaaag aattagataa gaagtgatat
     2041 gagtgtttaa accattaatc gattcatatt cagcggttct gaaaaagttc aaaggaaaag
     2101 acataggtgc aaccatcaat gaggaagtga acattgatcg actaaagacg atgtatgacg
     2161 gctacgatgg tgatcgagtt attgaaattc gttttattga tcctagacgt ttcacagtac
     2221 agcaacgaaa cttcatctat gcactcatag gcgatatttt catcgataca ggcatgccaa
     2281 cggacttctg gaaggaattc ttttacttcc gctttgaagg tgtcacaggg cgcaaaataa
     2341 gcctcaaaga tgaatcgaat acgactgtga gtgatgctaa cgtcttagca aatatcatct
     2401 tagatttcat ctttgaacat catattcctt tcaaagaagg ctatgagatt ttaccagcga
     2461 atcaagaata ttacttctac aaatgcatca caaaaagagt ctgttgcatc tgtggcaaaa
     2521 caggagctga tatcgatcac tttgacaaag cgctaggaag acgaaagcgc aaagaagttg
     2581 atcattcaga gtacacattt gcagcactct gcagaatcca tcacacggag aaacacaaga
     2641 taggtgtgat caatttcaaa aataaatatc aaatcaaagg gatcaagtta aaccaggaaa
     2701 caattaagaa acttagaata ggaggataaa aaatattggc tgacaacaaa cgatactact
     2761 atttaaaact aaaagagaac tttttcgata gcgacgagat ggttctttta gaaagtatgc
     2821 cagatggata catttattct aatattcttc tcaaacttta tttaagaagt ctgaaacatg
     2881 aaggcaaact aatgtttaat gacaggattc cattcaattc tacaatgctt gcgactatta
     2941 caagacactc tgtgggagtt gtagaaaaag cagtacaaat atttcgtgat ttacaactta
     3001 ttgaggtatt agataacgga gcaatttata tgtctgatat acaaagtttt attgggaaat
     3061 cttcaactga agctgatagg aagagaaaat acagaaaaga aattgaggaa gcaaaacaga
     3121 atttaataac aagtggacaa atgtcggaca aatgtccgga caaaactaca ccagagttag
     3181 agatagagtt agagaaagat atagagttag agaaagatat agagaatgta acgccttcga
     3241 aaaaatcgaa ggctaagccc gtccgtcata aatacggaga gtataaaaat gttcttttgt
     3301 cagatgagca gatggagaaa ctcaaaatag aattccctaa tgactaccaa gagcgaatag
     3361 aacggctatc tgagtattgt gaatcatctg gtaagactta taaaaactat ttggcaacta
     3421 ttcgaagctg ggcaagaaaa gaaaaaagtg aatctaagaa cgcaagcagt ggatacaagc
     3481 gcacaggaag acgagagaag cttcctgagt gggcaatcga ccaagaagcc tatctcaaga
     3541 aaaaagcgct agaacgagct aatagacaat caaaagcacc attctaagag gtgggaaatt
     3601 gaaaatcgat tatctagaac taattaatga aatagcaaag tataaaactg gtgaggaaat
     3661 agaaatcctg agagacgtat atgatcaact cgaagaagct ggaatcgaag gaattaagaa
     3721 tgatcgttcg agttggagta agctcagata ctatttcgca ctttatatcg atacaacaca
     3781 attaagaaat ttagcatata ccaaattact atttgttgat tgtgttaaag gcttgcaaaa
     3841 acatcttaat gaacttgagc aggtgtaatc agatggacct aaagacattt acagcacaga
     3901 ttgaactaat gcatcaagaa gctttaagac aaagtgtatc gtacgaagac aagtggctca
     3961 acacgttcca cggtggacgt gagagcgcac ttgatcaagt actcaaatta ttgaaagggg
     4021 aacgtcggga tggataagaa agcggcttgg cgaaaattaa tgttgctgat tcaagatgag
     4081 aactggcaag aagatgaagc agtggttgct gaagttcagc gtctagaaaa gattgctaac
     4141 ggacgtatac gaaaaaagcc tgataagcgg aaacttcgca aaggaaaaat catcgttgtt
     4201 ttgtatgaag gtaacatttt gatgcaagga acggcaagag agttgtctgc agaaactgaa
     4261 tatacgagcg gaactattcg gacgtacgcg tggcggaatc acgttgataa aaaaggacac
     4321 gaatataagt atttggaggg cagcaaatga taccgaagtt tagagcgtgg tacacaccat
     4381 ttaaaggtaa aacaattgga caagaaatga aatatgggca agcaggaagg ttgatcactc
     4441 atgctgaaat ggctccagat aaatatgtgc ttatgcaatc cacaggactg aaagacaaga
     4501 acggtgtggg gatatttgaa ggggatgtag ttcttgtcaa tgtaagtaat ggttttgatc
     4561 atttagttaa tgaaaaaacg gttgtacaag aatccgagtt tcattctgga ttagtttgta
     4621 aatcattaat tagtggaatg gaatatagag tattcaaaca caaaaagatg gattatgaat
     4681 atgaagtcat cggaaatatt tacgagaatt cagagttatt ggaggaacag cgatgaataa
     4741 acaggaaaca atcaaaaagt tagaaagtat taaagcgata ggaaatgatg caatagctgc
     4801 ttgctataac gagagtatga atgcaggtat tacgttaatg aaaaaaatag acgaaccgca
     4861 gaaacacgtt atgccgaagt tttttgacaa atgggcaaaa caagtcctgg aaaaacgtga
     4921 caagttttat gccatttctc ttattgcacg tgcaggttgg ggatatggtg ttgattttga
     4981 acttagcgag aacggattat catcagaaaa caaagagctg ttgtactggc ttgttgataa
     5041 atgcagcgac aattatccta ataaaaagaa agcaatagaa gctttgttat acggctacga
     5101 ggtcgagaaa gaaccgttgt atcacgtgaa gctaaaaata ccaggcgtaa catactacct
     5161 tatccaaact tttttaatgc caggagatac tcatatgtgt ttctcagcgg ctacagaacg
     5221 ttatggggct aaatggaaaa atacttttac ggaatcagaa ataaaagcaa ttgatgaaag
     5281 atactggcca tttgctgtgc cagtggaaga ggaggcagaa ggatgaaaaa attgcaccaa
     5341 ctcatatctg agaaagaaag cgaactccaa aatttagaag atagtttggg attggggttt
     5401 ccaattgttg agcaagctaa aatgactcaa atcagtcatc tacgacttga attggaagat
     5461 ttaagacaaa tagaaaaatc aattcaactc aacgacaacc agcaaatcgt gtttgagtgg
     5521 ttgaaactaa ctgcgccaac aggaaagcca atgcaagttg tattttggat gatgaataat
     5581 gcagcatggg ggcatttaga cgaattaagg gatcctttga tggaattaac agacaaagag
     5641 caattcgaag tcctagcagc attcgctcaa tggggattag aacaggagga agcggaatga
     5701 acaacagaca tcgcagaata acgaaactaa gaaaacagga actgaatgta ctaaaggcaa
     5761 agttcgaaaa agaatacgga gtttctgcag aagaagcata taaagtggca agtcagtgtg
     5821 ttgctgatgc gagtaatgct attcgtaagt ttggaatttc gatattaagt gatgatcgta
     5881 aatgggagga tatagaatga aggtttatgt agtaaggttc gggaataaat tttatagaag
     5941 tgatgaacgc tctataggca ataatgtgtt attccttgta gattttcttc aaaacgcaag
     6001 atggtttgat aatctcgaag aagctaacca agtttcacga cgacttggtg gattaacgca
     6061 agtatatgaa ctggtcacta tcgatcatga gggggtagaa agatgaaact aaaagacgga
     6121 ttttacgcta gtagtcatgg tatcggcggt ttaatgctag gtatgccgac aaagaaccct
     6181 aaaacacgta agaaaccaaa attcaaagtc ggtgacatgg ttcgctgcga agcagaggag
     6241 ttcatctatc cgtttcgtgg atatgtagag catgtttata atcactcagc gatcattcgc
     6301 attgaaaaca cgatggaatg tgacaagcgg ttagcgaaaa gcaaagagaa tttagcagtg
     6361 gtgagattgg tggatatgga agttataaaa tcttaatttt gcttttttac aactatccta
     6421 caacaatgag cgttattcca caactggaag gagaagaggc aattgtgaac attttagaga
     6481 atatagatat taagcaaaca aggaaaaatg ctagacgatt actaaaaaga tacagaaatt
     6541 tagaacgttt ggtcggacca gtgaaaatag acttttccgt gatgactgtt accaaaaatt
     6601 tgaaattcac aattgacagt caaaacgaag aaatcattga agcgataagt actcgagatt
     6661 cggttatcga agcattaaca cggctaagta gaatccattt ccaggtactt tattatagtt
     6721 attgttttcc taataagatg tcaatgtatc aaataggaga aaaattagga tattccgata
     6781 gaactatcga gagaatgaag gcagtagctt tggttgaatt tgctgaggcg tataaatcag
     6841 gagaactcat ttcacgtaca aaataaaaaa agtcggattc ctccgactat gaataatatt
     6901 tccgacataa gtattatatc ataattgggg gaatcagagg atggtacttt tcgatgtaaa
     6961 gaaatatgag acaccaagcg cgaaagatgt tgatatggaa cgcacaaaac ataatgtcgc
     7021 tgtgtttctt tcagcatatc tatcagctag atgtagagta ggtcaacctc gagaaccaaa
     7081 agtgacagca tcttattcct tggttccacc ttctacagct gatcatatat ttgaagcaga
     7141 aagaatgctg atcgataaag aagaagcaca ggaagaattt gagtatctgc ataaattgtt
     7201 tgtccgaggc tattcagcga tacagcatcc gcataagcct gatgtgactg aaaggcgcaa
     7261 gaagatattc tatgatcgtt atatcaatgg catgtcgatt tatgtaactg cccaaagaaa
     7321 taatacgagc gaggaatctg taaaagcaga atcaaacaaa atcatcattc aatttgcttc
     7381 ttccttagaa ctggttgctt tcaagtagcc agtttttaca ctttttatac ccttttatta
     7441 ccaatttggt ttccatttta tacctttttt ataccaatca cttacctatt caacgttgta
     7501 ttatgatagt gtcgaaagat taggaaacag gatcgacaaa ataaaatgta agggaggaaa
     7561 tctccctcat cgtttaatta agcttcgata ggcagcaacg gaaatattaa gaataaggat
     7621 gtgaatttca actccttcta aattgttctt attatctatc atccgttgct gtctattaat
     7681 ttatgtattg gagggtaaga atatgaaatt atcgattgaa ggtacttcag gagaaataaa
     7741 agaaatcctc tcagctattt gtaatagccg agagattagc aatttaatca atatagataa
     7801 tttaaatgaa tctattgtta ctccaagttc ccgagagaaa ttgcagaaat ctgatcagca
     7861 aaaacaacta aaaatgggat attagtagtt ctctcgccta tgatggtaac atctttcaga
     7921 aagatagatt tgactaccaa actttgctca tcatcttttt ctatatcatc aatttttttt
     7981 gagcgaaatt ctgcaaaaga atccatcata gcatcaataa gaggatttac ttttacttca
     8041 gcatcaaagt ctataggagt tccgcaaatt gttgctcctc cagtttgaat aataatatta
     8101 ttgttagaat ttttagcgaa gagttcaata tattcaatca tgtctgaacg gtaatcatca
     8161 atttttgcca tttttttacc accttaaatt atttcagcgg accactcgct gataaataaa
     8221 attatacgct tagtatttat tttcacaata ttaatttatc gtaataactt ttgagtaaat
     8281 agaacaaaaa acctgcacta gtttccgcta gtacaggcag tgactatatc ggttgataat
     8341 ctagcatata ttaaaaataa ttgcaagaaa agaaatttat agtggtttat accaaattat
     8401 caaagcaatg ttattttgtt gctgtctatt gttttttaat tattcacacg ataactaaag
     8461 gtgggtgaag agaaatgatt ccattaataa tttcaatttt tgcgctctgt cttaatgtct
     8521 atatgattgg atttaaaaat gggcaaaata aaaaatagta gcagccaaga ataattttat
     8581 agtgtcactg tggcggaaag ggtagacgct aagcatgtgt gctaggtcaa tgcttcggca
     8641 accatgcaag gttcgattcc ttgccagtga ctttggcaac cgaggcatcg gcggtttaaa
     8701 aatatagggg tgcgcaattt cgtacgcgtt ttgtgcatcg tgtaggttgc tattacataa
     8761 ctggttaggt tagattgaga tttgggattc ggtacaaatg aatcgtcaaa tgtctcaagc
     8821 acaggatcgg aaacgtccct gcctgtgcat tacatattag atcactcgtt gagtggtctt
     8881 tttattttgc aaaaaggagg taacaacaat gtatagacca caatacttag aacagaagca
     8941 tgaagtaatc actgtgcaaa acggtaacgg tgagatagta cgaaagtata gaagaccaat
     9001 aaagagcaat acatataaac gaaaggaaag caatgaagtt attccattgt atggcaaaag
     9061 aatagctaag tattaaataa gattgcgaaa ggagatggga catgaccgag gaattctata
     9121 gatggttatt acagttgata agagaagatc gtttggttaa gttctatcag tctcctaaat
     9181 ggcgcaggct tagagagaaa gcgatgaaac gagatcacta tgaatgccaa gagtgtagaa
     9241 gactaggtaa gtatcataga gtagagaacg ttcatcatat aaaggaagtc aaggatagac
     9301 ctgacttagc tttagattta gataatctta tttgtttatg tgttgaacat cataatgaag
     9361 ttcatggcag atatcttaca gcattagata aacaagagaa gaagatagaa agctttgcta
     9421 acttcgatgc aagtgaaagg tggtaagtgc atgatcatca atgacaatgg cagagagtat
     9481 gacacagaaa agattgaaga gtattcatct tatactcaag gattaattaa acgtttgata
     9541 tacgttcgct atgtaggtat tagggatctg ttatcagata actgttgcag taaatacaaa
     9601 gtgaatcaag taagagaagc gttgaataaa gataataacg ttgaaagaat aaaaaatatt
     9661 tttggatata gcattgaaga gattaattat tacattgact tcgctgaagc tttcattccg
     9721 atggtgagat aaccccccct taaaataaat cgcaaatttt ttgggggtga tgaaacggag
     9781 ggggctgtca ggaaaagaga ttttttcgaa ctttatcatg aaaggagggc taaaatgttt
     9841 aaaaacgaat tgtctcaaaa tcgctacaga gaaaaattac gccgctcttt aataagccaa
     9901 ttggaaagtc agaaaacaaa tattgagcca ttcttagata atgttgatcg ttatatcagt
     9961 ttatgggaaa cggcgatatc actggaagaa gatatatccg agaacggcat tagactggag
    10021 aatggtaaaa agaatgaatc agtagcgttg cttgtttctg tcaacaaaca aatgggattg
    10081 atgttggata aacttgccat tactcctgaa ttggtaggtg aagcaaatga atcaattcct
    10141 gagttataag catattgaaa attggttcaa agctatagaa gaaggcacta tcaaggtatg
    10201 caaagagcaa ttattgctaa aaaattatct agaagaaaga gtctttacta gagaagatat
    10261 ttacttcgat aagcagatgg tagaggattc aatcaatata ccagcacaat actttccatt
    10321 cgaattaatt ccgtgggaaa aatttctaca atgttttatt tatggtgttc gatggaaaaa
    10381 agataaaacg ctagtgttca atagatatct ttcattaatg ggacgtggta atggtaaaac
    10441 tggttttgct tcttggaaca attttttctt gctgactgca aaacacggta ttaaaaatta
    10501 tgatattgat atctatgcca ataacgaaag ccaagcaaag actagttttg atgatgtatt
    10561 taaagtaatt aaagatcatc ctggtttaga taaaaaagta tttaaagcta cgaaggaagt
    10621 tattcaaaat atcgctacaa acagcaaact tcgttataac acggcaaacg ctagaacaaa
    10681 agatggtaaa cgaccaggag caaaccgctt tgatgaaatt cacgaaaatg aagattattc
    10741 aatgataaat gtagctactt ctggtggtgg taaaattcga gattatagag aattttatga
    10801 tacaactaat ggtcatgttc gtggtggtcc gcttgatgac attatagaag aatcaaaaat
    10861 gattctttct ggagaacttg gaattgacaa ggatggagca gaattttcta gtttgtttcc
    10921 atttatttgt cgcttggata atgataatga agttgatgat cccgatatgt gggaaaaagc
    10981 ttgtccaact attaattaca atgcagattt aaaacggaaa atgtttcaag aatactctca
    11041 aatgcaacgt aatgctggtt taagacttac gttcatgacg aaacgaatga atagaccaat
    11101 ggaagatacg cgatttgctg tagcttcata tgatgatgtt cttcatacga aagaaaaaga
    11161 atttcctgaa aaaatggatg aagtgatagg aacagttgat tttgctgata gacgagattt
    11221 tgccagcgtt ggtttgttag gaaaatatga taaagatgtg tattttacac aacatacttt
    11281 tatccacgaa tcagctcttc gattacagaa cataaaacga gaggttatag atatttctat
    11341 agatcaagga aaatcacaga tcgttcatgg gaaaaatata gaagctgatt atattgtagg
    11401 ttggtttctt gaaatgagta ataaatatta cattaaaaaa atcgctatgg atatgtaccg
    11461 tgcaaaaata ttgaagcccg ctttagaaga agcaggtttt actgtagaaa ttgttcgaag
    11521 cggatctgtt acacatggta tgttaaaaga cctagttgat gaccttttta ttaatcaacg
    11581 tttatttttt ggtgacgatg cgattatgcg ttggtattgc atgaatgtat atgaagagca
    11641 tatttctaat ggaaatatac gctatgaaaa aatagaacct gaaactagaa aaacggatgg
    11701 ctttttttca ttccttcatg gtttgaattt tttagatgat atttatgatt ctgctcctgt
    11761 aacagtcaca aatagttcag tagaaaatgc aggaactgga tttactcctc tagtattcta
    11821 acttgaaagg aggtgagaaa gtggggattt ttcaaaaggc ggtaggatac ttcacaaaaa
    11881 aagcaacggt tcctttagaa gaatactttt gtaaattgca agttgatttt gtgtatcgaa
    11941 aatttgcaat tgaaacttgt attgatttga ttgcaaatgc gatgagcaaa gcggaattca
    12001 agtcatatga agatgggaaa aataaaaaga atgatcttta ctataggctg aatgtagctc
    12061 ctaataagaa aaataatgca acagaattta gaaaaaaact gatcaggaga ttaatattct
    12121 acaatgaagt attgatcgtt tctccgtcta ataattctag cgaaatattt attgcggata
    12181 gttgggatgt cacagagtat gcattaaaag atgatgtgtt ttctcaagtg caaattaaca
    12241 acatagtcct tgatagagaa tttctagaaa gtgatgttat ctatataaaa tacgcagatc
    12301 aacaaattag gcaactagtc gatgcgtatt atcaagcgta tgggaaactc atttctagtg
    12361 ccatgaatgt ttacaagcgt tctaacgctc gtagatacgt actgaaaggg aatttattcc
    12421 gagcgcaaga caatacaaca caagatcaaa tcaataaaat gatgacatca caatttaagg
    12481 cttttatgga agctgataat gcaggtgcgg tatttcaatt acaaaatgag tacaaattag
    12541 aagatttcag cggaaacttt caaagcaatt caagagatat aaaaaactta atagacgaca
    12601 tctttgagat gacagcagca gcgtttcacg ttccgaaaaa cctactaaag ggagacatga
    12661 gtggattatc ggatcaagtg gacgcttttt taatgtttga aatcataccg attgctgaac
    12721 ttattcagga tgcgtttaac gctagtctct atgaagtaga agaatacttg tcagggaatt
    12781 ttgtacgtgt ggatacaact atgatcaaga ttactagctt caaagatttg gttgacgcta
    12841 ttgatgtagg cattagaaat ggggtattta caatcaacga aggaagagaa cgcgttggaa
    12901 atgatcgctc tgataaggcg atggcagatg aaatatttat aactaaaaac aatcaacaag
    12961 tatcgaaagg aggtgaggcg aatgacgaca atgaaaacat ttctagcagt aaagaatgaa
    13021 ggcgcagtac cgcaaatttt tattcaggga tttattggtt ctagttggtt ctttgaaggg
    13081 aatactgaca agggaatcaa aaatattttg gatagtctag gtgatcaaga agagattgaa
    13141 gtagtaatta attcaaacgg tggagacgta tttcaaggga ttgctattgg gaacttactt
    13201 aagtcaaata aagcaaaagt taacgttgtg attaacggct tagccgctag tgctgcttca
    13261 attatcgcaa tggctggcga tactataaaa atctacaaca atgcacaatt gatgattcac
    13321 cgcgcttcca catacggaga aggaaatgtc gatgacttcc gtacgattgc tgaccaactg
    13381 gaatcaattg ataaatcggt aaaggcttca tataaaacac gattcaatgg cacagatgaa
    13441 gcattgcaag aacttcttga aaaagaatcg tttatggatg cagaaacagc tttgagttat
    13501 ggattggtcg atgaaattat cgatgcagaa aatagctcag gtactgaagc taaaaaagaa
    13561 caaagcgttg aagaaatttt gaatgacgtt aaagaaaaaa gagaagaaaa aattgctgca
    13621 tttacagcag cattaaataa aacatttgga caaggagatg taaaataatg acagttaaaa
    13681 atttaaaagg tgtaacagct gcaagcgacc aattgatgaa agcttttaaa gatggtaacg
    13741 aagaatcttt tagcgcagct atggtaagct tatctaagga aattcaggat aaaattttag
    13801 aagaagcaac agcaaaaaat caagatcaat tagtattaat gaaccgtggt cagcgtgtat
    13861 taactacaca agaaacaaaa ttctataacg aagtagtgaa aaacgaaggt tttgcagggg
    13921 ttgaagaatt agtgccagct actgtatttg aacgtgtatt tgaagattta gaacaatctc
    13981 atccactatt gcaaaaaatt acttttgtta acacaactgg tgtaacagaa tggattgtgt
    14041 cacgtggagt caatccagca tggtggggta aactttgcga agctgttaaa aaagttttag
    14101 ataatggctt tgatgtaatt aacatgaagc agttcaagct atcaggttat attcctgtat
    14161 gtaaggcaat gcttgattta ggtccagtat ggttagatcg ttatgtccgt actgttttag
    14221 tagaatcatt gagaattgca ttagaacaag caattgttga tggtactggt aaagatatgc
    14281 cagtcggaat gatgcgtgac atgagcaaac aaactagcgg agaatatgct gaaaaaaaag
    14341 cagaacctat tacagcttta gatgctgtaa ctatgggcgg tttgatggcg cgactatcaa
    14401 aattcaatat cgaaggtgtg aatgatccga tttatcgtaa tgtgaatcct tctgatgtgg
    14461 tcctaattgt gaatccaact gattactggt ctaaagtatt cccagctaag actgtactaa
    14521 ctgctaatgg agaatacgta caagtattgc cagtaccagt ttcagatttg cagtcaacgg
    14581 ctgtgccaga aggaaaagca gttattgggg tagcctcaga ttacttcatg ggtgtaggat
    14641 ctacactaaa aattgaagct tcagatgaat accattttgt tgaagacgaa cgcatttatc
    14701 tagctaaaca atatgcaaac ggtcaaccta aacgtaacga tagtttcatt gtattagata
    14761 ttagcgcttt gggaactact actacaacta caaaaccaac aaccacaaca actacaacac
    14821 gagcgtaggt gatcagaatg aagtatattc tttgtcagcc ggcaatcaat cggtttaaat
    14881 gggagcttga agtttgttta actaatctga agaaactagg aatcaaagat atcgtattgc
    14941 ttttcagcag acacgatgat cagattccta ttttttttga gaaggaatat ggtgttgaag
    15001 ttcatgtgta cgatgatctg cgggacgaca aagagtatat tccttcgatt aaaccatatt
    15061 tatggtggaa atatttagaa gaagatcatt cgcgtgagga cgaccgatat ttctatatcg
    15121 attcggatgt cattttcaat aaaagaatta atttgcgcaa attgccttct aaagatgatg
    15181 tttggtattg tagcgactgc tgtagttatc taagtcttga ttatattaga agctgtgaaa
    15241 acggagaaaa tattctaaaa gatatggcaa acattgtaaa tgttacagta gaatctttgg
    15301 aaactataaa cactaattca ggaggcgcac agtgggttat taaccgtcct aaagcgaatt
    15361 attggaaaaa ggtttatctg gattctaatc ggctatatcg ctaccttaga gggcaaaaaa
    15421 caaatataca aatctggaca gccgagatgt gggcacagct ttggaacatg atgtatttca
    15481 atattggtcc taaagttcac gaggaattag acttttgttt tgctactgat ccaatagaaa
    15541 aagttaaaga agtaaaaatc ttacacaatg ctggagtaac aacaaacgat gaagatttat
    15601 ttttcaaagg gagatacgtg acttctacgc cttttgatga agatttatca tttgtaaaca
    15661 agaaaaaatg ctcttacgca tatgttaaag caattaaggc ggtggttaga tgacgcctga
    15721 acaagtgact gaagaattgc taatagctgt gaaggataat atttacgtta cctggaacga
    15781 agaagatgag tcaattaaaa agatgatagc taaaaatgct gtttatcttc aaagtaaagt
    15841 gagtacaaca ctttcttttt ctcctgaaag cttagaatac ggattgctaa tcgaaagatg
    15901 tagatacgac tggaatcgtg ctttagatga gtttgaacaa aatttcgcta gtgagttatt
    15961 aggtttcatt caacattatg cgctacaaga atatattgca ggtgatgtga atggcgaata
    16021 atcgtagact cgaagaaaca ttcaacgatg gttggttaaa gattttgacg caaactacca
    16081 aaagaaatga actaggaaaa aagattggtg tagaagatac agaaatcact tctttaaaat
    16141 ttagaaatct ttccatgaga gatagtgata taacagctat ggatgcgatg ggatcgaaat
    16201 taactaagaa agtaaagact ccatttcatc caatcgccaa gaaatttaat aaagatcaat
    16261 attttatcgt aatcgatagt atgcgttaca acgttatcta tgccgattac gataattttt
    16321 atatctattt ttatcttgaa agtgtgggtg aatatggtga ttgataattc taaagaaaaa
    16381 gaacgtttaa ataagcaaat ttctgctatc aaaacttcct tagaagagca ttttggcctc
    16441 aaactctttc aagactccgt aggcgaggat gagctacctg atgattttaa ttacttcatt
    16501 ctcgaaacag gagaaataga aatgatcact gagccaaaat atagcgtggg tcaaaatcta
    16561 tatctaactt tctattcaga aaatagagaa gatttaacag gagactcact agatattatt
    16621 tcattgattc aaaatcgttc gattcgtttt cagagaatgg atcccaatca tttaaaacta
    16681 gagaaccaag atcgctatat cgatcaattg gtatttacgt ttagacgatt attgaagagt
    16741 gattgtcatg gctaaaaata gttgggagct aaaaataaat ggacatgatg aacttcttgt
    16801 gcggatggaa cgctattcaa gcgagagcga acgactgatt aacgaagcat tgaaatcaaa
    16861 aggttcggct attgcagtgg ataggattac agaaaaaatt cctgtttctg aagcagattt
    16921 aagaagaggg caccaacacg caaaaaatag tcgtccactt aagactcaat acattaattt
    16981 gggtttcatc attagaccta caagaaaatt tgagtattta aaatatcctg atttggggat
    17041 aggtacttct aaaagaaatc agccggacga atttatgaga agaggattag gtcttgcact
    17101 tgatccaatt acagaacttc tgattcgtca attcgataaa ttaaataaat agggggaaca
    17161 acaatggcta aaacaaaaac tgtagtaaca acgttcgata acgtaagtat caaacgaatt
    17221 gcttttaatt ttaagaacgc aggaaatgca atcgcaacga attgtaacgg acaattagat
    17281 ggcgaaacag aaatgcaaac ggtggttaaa aaatgtggag cgacagaagt aaaatcaaaa
    17341 tctaaaccaa tcaatatgac ggtaacaatt actgcacatg taccgatgga agtttatcga
    17401 cgtttcaatg ggttgaaaca agatggacgt attaaaccag gcatttactc ttacggtcct
    17461 gattccgtag gcgaagattt ctcacttgct gcagagatcg tggatgactt cgaagaaaat
    17521 agaaagttag ttggtatgtt agcatgcact tcgaatacag gattaacatt ctctattgaa
    17581 aatggtgcgg atgaagtagc tgcgttagaa ctagaaacaa aagttatgca agatgaattt
    17641 ggtaaatttt atcatgaagc aattgttgca gaacttgaag aagacttaac agatcaatgg
    17701 atgacgaatc tatctgctga tgtgattaaa aaaacttcag ttgtgacaac tacggccact
    17761 caatcacagt aaaaaaaacg gaggtagcga aatgaacgaa gattactcaa aaattgaact
    17821 aaacgatgga acaattttga atttagaacc taaactgaat atcaagaaat tattgatgat
    17881 caatagagat tttaacacag acgagtttgc aaaaatgact gtgggaaaag gatccatgga
    17941 tatttctgtt attcaaggtg caaaggctgt gtatattgct taccgccaag cgaacatgac
    18001 tgattatatt tcattcgatg aatttatcga taaatgggat tttgatatgg ctactgccag
    18061 ctatatttat caattgatga tgttcaaaca agcacgcgat gcttatcaaa aagaatttga
    18121 aaaagcaaat aaggaaaaaa agcttcaaaa gtaaaaatgc caaagctctt agttgaaacg
    18181 tgggtcgatg tctattcgat gttgaccgac gttttttcta tgccttcaga tttggtttta
    18241 agcgatatct gtttagatga cattttgcaa atggcttaca acaagagtgc ttatgaagga
    18301 tggaaaaact atgcaataaa ccaatctcag aaaaactaaa gaaaggaggt aaaaaatggc
    18361 taaaaagaga acagaagcag aagtaacttt catagctaac gatgacggat tgaaatctac
    18421 gttaaaagaa atcagtgctg aattaactaa aaatagagca gaattaaaac tagaacaagc
    18481 tcaattacaa cagactggtt ctgaatcaga caagttagga agtaaattat cttctttaga
    18541 aaagcagtat gaattacaaa gccaaaaagt tgaagtaact agccaacgtt tagccaatgc
    18601 caaaaaatat tatggagaaa attccaccga agttcagaaa cttgagagag aactgattaa
    18661 ccaacaaaca gcgcaacaac gtttgtcaaa cgaaattgat aaaacgagta atgcactagc
    18721 tcaagcaaaa ggcgaaatac agacgtacga gtctacaatg caacagttgg atagtgaaca
    18781 aaaaaatgtt caagctagtg cttctctgat tgaatccgaa tacaaaaaat ggcaagtaac
    18841 tgctggtcaa tcagcttctg aatccgagaa attagcgaaa gcccaagaat atgtttctca
    18901 acaatctgaa aatgcagaga aaacgataga tatcctgaga cgacagttag aagctacaca
    18961 gtctgagttt ggcgctacat ccacagaagc aatgcagatg gaggcgaagc ttaatgatgc
    19021 tgaacgtgaa tttgaagagt taggacaagc tgctaaaaat gtagatacaa ctaacttgga
    19081 cgatatcgga agcaaaatag atatgaataa tttaatggaa gcttctgacg ttttaagcga
    19141 cattggcgat aagcttacag aattagggaa acaagcagtg gactcagcta acagtgtagg
    19201 tagttcccag agtaagatac aagctaattt tggtttgact aaacaagagg ctgaagaatt
    19261 aacgaatgta gccagagaca tttattataa aggttttgga gaatcgttag atcagtccac
    19321 agatgcattg attttggtaa agcgtaattt aggcgattta aataatcaag atttacaaaa
    19381 tatcacggaa caagctatgg tcctagaaaa caccatgggc gctgatatgg atgaaacgtt
    19441 acgtggtgta aatggcttaa tggtcaattt cggcttgagt gctcaagatg caatggattt
    19501 aatggtttcg ggtactcaaa acggtttaga taaaacgcac gaattaggcg acaatatggc
    19561 agaatatagc caattatgga gtcaaatggg atattcagct gatgaaacgt tcggaatgct
    19621 tcaaaatggt ttagatgcgg gtgcttataa ccttgataaa gtcaatgact tagttaagga
    19681 aatgggaata tcgttaacag atggtcgatt tgagcaaaac atggatatgt ttagtgaaag
    19741 tactagaaaa gcttttgaag agtggaaaaa tggcggagga acacaaaaag acgttattaa
    19801 ttccatgatt caagatttta gcaatatgga tggtcaatac gaccaattaa ataaagcttc
    19861 tacaatttgg tctgcacttg gcgaagataa cgcgatgaaa gttgtccaat ctttgactga
    19921 tgttaaccat acatttgatg atgttagtgg atctgcacaa aaaatgaatg aagattctac
    19981 tactccgtta caagaattaa acggaaaaat agctgaatta aaggattcat tagctcctat
    20041 aggcaacaca atcatagatg cactcgaacc agtaattgat tttctaggaa agatggctga
    20101 tgcgtttaat aatcttccac aaccagtaca ggattatgcc gtagcgattg gcggattgac
    20161 tgctgcattt actttattaa tgccaataat agttggcttc atggctctag gtggtcctac
    20221 tacattaata ataggagcag ttattactgc tattgctgga gttatagcaa ttataaaaaa
    20281 ctggggcgca attactgact ggtttaaggg aatatggagt aaattcactg attggttggg
    20341 tggtacttgg gaaagcataa aagaaggtgc ctcatcagtt tgggatggag tcaaagaaac
    20401 ctggtctgga tttgtagatt gggttcaaga aatttggcaa ggagtttctg attggtttgg
    20461 agagttatgg agcggattag ttgaaggagc ttccaacatc tggcaaggag tccaagagac
    20521 ttggcaaaca ttcattgatt gggtttcaaa tatttggaac ggagtcaaag aagtatggtc
    20581 gattatttgg gcagacattg taggaattgt tcaaatacca tggacattaa taacgtcatt
    20641 gattcaagct ggtattaata ttatcgtggg tatttttgat gtagctggac agttattagg
    20701 cgcagcttgg caagctgttt ggacacctat ttctgatttc cttaaaaata cttgggatac
    20761 tatgacacaa tggataagca tcgcttggaa tggaattgta actacattcc atactatatt
    20821 tgatccagta gtggcatggt ggaatggtat atggacatct attagtacta cggcttcaaa
    20881 tatttggaac tcaattagtg caacagcttc tagtatttgg aatagtatca agaatacaat
    20941 cactagcttg gtacaagcag ttgctacagt aattcaaaat atttggtcaa ctgtatctag
    21001 ttggttaggt ggaatttgga attcaatcag ctctacagca tcaaatatct ggagtagcgt
    21061 gactagtagt ataagcaatg ctataaacgc agctaaaagt gccattcaaa gtgtttggaa
    21121 tagtatatct tcgtggatca gcggaatttg gaacggtatc aaaaacactg ctttgaatct
    21181 ttggaatgga attacaagca ctattagctc taaagtaaac gatggaaaaa atgcaatttc
    21241 aagcggttgg tctaatctaa caggtattgt ttccgacata ttcaataatg ttaaaagcac
    21301 gatagctaat atatgggaag gtatcaaaaa gactgttagc gctccgattg attggattag
    21361 agataaaatc agtagtgtct ttgataattt gaatatttct ataccacata ttccgttacc
    21421 acattttaaa ttgagcggag aattcaatcc attgaagggg aaaatcccaa cattgggtgt
    21481 tgattggtat gcgaaaggta gtgtgtttaa ttctccaaat attatcggtg tcggtgaagc
    21541 aggacctgaa gcagttttac ctttgaaaag atctgtgctg caagaaattg gggatcgtat
    21601 cttgagtagc acctcagttt catctagggc acaaacgatt caacctgtga acaattacga
    21661 attcaatttc acaattgatg gtaacgcaga tgaagtcact atgaagcaaa caactcaaca
    21721 aatcattgat agcattacaa aagttcaaaa tgataatgct tcggcatggc gttaaacagg
    21781 agagtatttc tcctgttttt ttagttttaa aaaggatgtg aaaaaatgac tgattgtata
    21841 cattctataa tcgatggatt tcctgattat ttgcataaat tggctttagc ggaaagacca
    21901 accatacctt ctccaaaaag acagagagtt gaaacttctg ttttaggacg gctaggtggc
    21961 ttagtacaag attactcgtt tgaagacatg tcgtttacat tgcactataa ctatttagaa
    22021 gatgtggaag accatcaagc attcaagcaa tcgttttata tcatgcgtca ttggttaaac
    22081 tatgcaaaga aattagaatt ctctgatgat cccaacgtct attatgttat ccagactatc
    22141 gatattgggg atgcagaaaa cgatattgtt gaatggggag agttcgatgt aaatatcact
    22201 gcgaaaccat tcgcaagagt tcaagaagat gtacctataa ccgtagataa accacagtca
    22261 tttaacttgc tgaataatag tttagaagaa agttttccaa agattatcat cactccttca
    22321 gctacttcat gccagttcat cttaaatgat tatgtgttta gttttgaagg cttagtagta
    22381 ggaactgatg tagtcattga tagtgattta atgctttgct acgaagagca atcggacgga
    22441 gatattttag atcggtccaa caaaatgaag accatgcaat atccaacgtt gcaagtggat
    22501 attaatcatt ttacttgtac tggtttgagc aaaatacaaa tttatcgtaa tgggttaagg
    22561 taggtgaaat agatgatcga taatttacta actatttacg ataaaaatga cgcgaataat
    22621 ttagctgaac atttatatga tacgcaaggt ttaggcgctt tgtcagactg gttaacagct
    22681 actgttagca ataaactaaa cggagccgag atatttcagg gtacttattc aataaacgga
    22741 actaatgcag atttgattgt agaaggacgt attattcagt gttatgtaga tgaaaatcga
    22801 gcaaaacagc gtctacggat ttattatgca aaaacttctg taataggcaa tacgatagaa
    22861 gtaaaagctg aacctatttt caatgatata agaaaatcgg tgttgaataa atatgacagc
    22921 ggaacagaaa aaatcactgc tactcaggca tggcaaaacg caaaagtttt agcgaaacca
    22981 gctatccctt cgcagttttc tttctcgtca ttagtagata cgcttgctaa tgtgaagata
    23041 gaaaaggcga attttttaga attctttggt ggaaaagagg gatctattct agatcgattt
    23101 catggtgagt ttctaaaaga taataacaca ttacgtcatg aaaaaagtct aggtacggat
    23161 cataaaatca aagcgattta tactaaaaac ttaactggtc ttgacttaga gattgatgct
    23221 caaagtgttt tagttggagt ttatccattt attagcagct cttcagaagg agaagacgag
    23281 atcactctac cagaagaagt tattttcacg gattacgtgg atgattatcc tgctggatat
    23341 gtttcttttg ttgattttaa agacaaagcg actgatgtag ccacattaag ggaagctgct
    23401 aaagactggt tgaaaacaaa catagataaa caaaaaccac aagtgagtgg ttcgattgaa
    23461 ttagtaccat tgaggcatca aagaggctat gaaaaatttg ttgatctaga aaaagtttcg
    23521 atgggtgacg gagtagatgt gtatcatcca cagttaaaag tgaatatgtc agcgagaatt
    23581 gtggaatata cgtttaatgt tctaaccaat tcatacgata aattagttgt aggaaacgtc
    23641 aaaacaaact tcttagaaaa cacagagaat aatgtcagca atttgattaa tgatgccatt
    23701 gatcaattga aaaacgttgg cgaaatcagt gatttaatca atgatattgt agatcatcaa
    23761 actgatatga ttactggtca aaatggtggt tatgttttat tagatcctaa agaagcgcct
    23821 agtcgtattt tgattatgga cacaccagat aagaataccg caaggaatgt tttacaaatc
    23881 aacaatgctg gtattggttt ttctaaaact ggcattaatg gaacatatga aacggcatgg
    23941 acgttagatg gcggattcaa tgcctcgttt attacggctg gtgaaatagt aggaattact
    24001 attagaggta ctacattaat tagtgatggt gctgattata gaacaagtat tgctaatggc
    24061 aaaatgactt ggtactcaaa aaaagttaac aaagatatta tggagctaga agcacgtgat
    24121 tatgtaagtg ctgatgccgg tattgtatca tacaccatga aaactggtgg tggtttcatg
    24181 attagaaatc cacagggtaa cttggttttt agtacgtggg ataatggtaa taacagaccg
    24241 tttctatctt ttggtgcgcc caatttcagg tatagcaatg ctagttatgt aacttctggc
    24301 gacggtagtt ctttaagcat taatggtagt gcgggtaact catgggaatt taaggtagct
    24361 ggtaggacta tgaaatttac tagtgatggt atgctaacgt taccaggttg tttttttggt
    24421 tcatgggaag atgggaaact tgctaggttt gaacaatcaa cggtacaagt atataaagat
    24481 tttactgtta gaggtactaa aaactcaact gtaccaacag aacattatgg acaacgacta
    24541 ttgaacgctt atgaaactcc agaatattat ttcgctgatt atggggaagc cgttacaggt
    24601 gacgatggta aagttcgtgt tgatattgac cccatgtttg ctgagacagt aaatctaagt
    24661 cggtatatga cacatgtgac acctacagaa ctagttttgt gtgctgttac tcatgaagat
    24721 attgaccatt tcatcattga aactagtaag ccaaacgtat tagttagatg gaatttagtg
    24781 gcacaccgtc tagggtatga agatattaga ttaaaagagg atacagcata tgatagcaca
    24841 gtgcttgacc aaaaacgttt ttaaaacgaa gacaaggagg tatataaatg gctagcagtt
    24901 tatataattt ggctttagat ttcagcaaag aattaaacta caccaaagct attatggctc
    24961 gtcagggtga taaagggatt acggtgacgg ttaaaccata tttaaatggc ttgcagatgg
    25021 atacgagtgg cggaacattt actttaaaag gaacaacacc atctaaccgt tacgtagata
    25081 atgttgcaac tagcgtaact agtgaagaag tcacgttttc tcttgatggc acatttatga
    25141 gtgaagcagg atattataaa cactgctacg tagaatatag aaaagacaat caaattctaa
    25201 caacgcaaga tatcattttt ttctcactag gagtgtctga catttcgcaa ggtcaagccg
    25261 atgaatatgt ttcgcaatta gaagagttga ttcgaaagta taatgaaact tttgatgctt
    25321 ttatggctga aactaaaggt agagtggata gcttaaatca acagattact gatttaactg
    25381 gtcaagctaa aacgctacaa gacaagttag atgctctgaa agaagaaatt tctaagttag
    25441 gtaacttaca agtgatgtac agtaacagca tcgatttcgg gaactatgat tatagtggga
    25501 atcctaattt gttaagtaag ctatcatacg acttaattga aaatcaaaat acttcagctg
    25561 gaacactttc taaaggtgaa aactcgttta aatataataa gatatcagct gaaacggaag
    25621 gtggagtaga gttatattat aaacgaagag gtatagctaa ctggttaccc tctaataaaa
    25681 cgcttgtaat gaccgttaaa cttagagctg gagttgacta tagtccagct gacggaaaaa
    25741 aaatactgat tagatatagg tttgttgaca atggaactgg caagattgtt ttagacttac
    25801 ctattaacag taattcgata actcaggaat ggaaagagtt tagtattact ggaactactc
    25861 caacatttgg cccgcaagca taccatcctt ggatacaatt tagggctcaa gatgggatac
    25921 ttggggaaat agaaatgagc tatgacatca aaatcgaaga aggtccaaca gccacaccgt
    25981 tccaacctaa cttattggca gaaccttata acatgtgccg cgagtatcct aacgagaaca
    26041 ttgtcaatcc tacagttaag ttcccaatta aaacatcaaa taatcctatt tctggttttg
    26101 atatttcaga agaattaata ataggtgaaa cttatacggt atctttaaaa gggactaagc
    26161 cagctactaa agaattccac ttatactacg gagaagcaaa ttaccagcag ttgcagcatc
    26221 aggcaacttt acttcctgtg gaaggattaa cagatgtgtg gagtgccact tttacagcca
    26281 gaaatactag tgaaacgaca aatttactta gagtagcgtt atggcaaaaa cctaattctg
    26341 caacatatga tactgttcaa attgactggc tcaagattga aaaaggcaac acccgaacgc
    26401 ccaatattag tgagtataaa tatcgtggta ctggtatgcg tgattcaaac aatccaaaag
    26461 attatgtttg ggatctagca ccagaatatg tcgaagataa tttggccaca gatattaaaa
    26521 tttctgaaat tactggtaaa gcaaacaatt ataccgatgg gaaagtatcg gagattaatt
    26581 cgcagttgac tgcttcaatt aatgaagtag acaccacagc taaggatgct cgaacaaaag
    26641 cgaatgctaa tgcgactgct atagatgaat tagacaataa gatcgatgaa cgcattaatg
    26701 atgcagctac taccgcattt tatggcgcac aaggtgaagt ctctaacaaa acgaaagtag
    26761 cggagtatgg agtaggaaca gggtatacta ctacagctgt aggagaaaca catttcgaac
    26821 gtcaatctaa cggatacgtt aaatgcttaa aagctggtaa gtattcaatt agtgcgcaaa
    26881 tacgtgtgca attgggcggg aaatatgctt catggttata tactgattta tataaagatg
    26941 gaacttctgt tgataattta gtatcttatg gggtcgacgc cttgcaaaat agatttgcag
    27001 ctagcggaaa cattgtaact gatttagaag taaacgatgt actaaatatg cgtactgaaa
    27061 tcggcgttgc agatggaatt ttacaattta catcttgccg tactttagtc ttgactagac
    27121 tatctgatta atacaatatt tataattttg ctatcaacac gctcaaagga gggtgttttt
    27181 tattttgcaa tgaaaggagg ctagttggtt gaaagacgaa gcaatacaag acgtggtaga
    27241 acgcttagtg cgtattgaaa cgaaactgga taattacgaa ttattacgtg aaaaagcgga
    27301 aagtgcaaaa gatagagcgg atcaggtata ttctattgcg cttaataatg cagaagacat
    27361 caaagaaatg aaagccaata ataaatggtc gtggggttac atgattggtt taggcattac
    27421 gatcattggc tatttcttga ctaaattgta aaggaggtga gaagaaatga ttttaccaga
    27481 taagtattat caagtcatta aatggacagt tttaacagta ttgccagctg catctgcttt
    27541 agtagccatg ttagggaaag cgtatggatg gaatggaaca gatatgatag tgctgactat
    27601 caatgcagta gcaacatttt taggcgttat cactggtgtg tcggcttata atttgaaaaa
    27661 ataggaggaa acaaatgaaa aagaaaatta ctattactgc gatgagcctg ttaacggctc
    27721 tttttttatt gccaattaac ggatttgcct atactatcaa caatgaattt aatttgggcg
    27781 caaatgaagg tagctcacaa gtagcaaata atcagtacat tttactgcat gaaacggcta
    27841 atgaaacagc aacaggacgc aatgaagcgc agtatatgca acgttcatgg actagtgctt
    27901 acactgctta cattgtgggt gatggcggaa ttgtttacca agttgg
//
LOCUS       DAGCYL010000019        24687 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.019, whole genome shotgun sequence.
ACCESSION   DAGCYL010000019 DAGCYL010000000
VERSION     DAGCYL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.019"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(77..1339)
                     /locus_tag="MB216_000640"
     CDS             complement(77..1339)
                     /locus_tag="MB216_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4525498.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1332..2231)
                     /locus_tag="MB216_000641"
     CDS             complement(1332..2231)
                     /locus_tag="MB216_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4525499.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2376..4154)
                     /gene="uvrC"
                     /locus_tag="MB216_000642"
     CDS             complement(2376..4154)
                     /gene="uvrC"
                     /locus_tag="MB216_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBT4525500.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4336..4650)
                     /gene="trxA"
                     /locus_tag="MB216_000643"
     CDS             complement(4336..4650)
                     /gene="trxA"
                     /locus_tag="MB216_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBT4525501.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4737..7097)
                     /locus_tag="MB216_000644"
     CDS             complement(4737..7097)
                     /locus_tag="MB216_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBT4525502.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7212..7760)
                     /locus_tag="MB216_000645"
     CDS             complement(7212..7760)
                     /locus_tag="MB216_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBT4525503.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(7818..8255)
                     /gene="zapA"
                     /locus_tag="MB216_000646"
     CDS             complement(7818..8255)
                     /gene="zapA"
                     /locus_tag="MB216_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBT4525504.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8457..9374
                     /locus_tag="MB216_000647"
     CDS             8457..9374
                     /locus_tag="MB216_000647"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBT4525505.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9752..10663)
                     /locus_tag="MB216_000648"
     CDS             complement(9752..10663)
                     /locus_tag="MB216_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBT4525506.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11064..11642)
                     /locus_tag="MB216_000649"
     CDS             complement(11064..11642)
                     /locus_tag="MB216_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4525507.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11662..12330)
                     /locus_tag="MB216_000650"
     CDS             complement(11662..12330)
                     /locus_tag="MB216_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4525508.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12345..13415)
                     /locus_tag="MB216_000651"
     CDS             complement(12345..13415)
                     /locus_tag="MB216_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4525509.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(13586..14950)
                     /locus_tag="MB216_000652"
     CDS             complement(13586..14950)
                     /locus_tag="MB216_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4525510.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(14947..16506)
                     /locus_tag="MB216_000653"
     CDS             complement(14947..16506)
                     /locus_tag="MB216_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4525511.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(16562..19042)
                     /locus_tag="MB216_000654"
     CDS             complement(16562..19042)
                     /locus_tag="MB216_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525512.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(19026..19361)
                     /locus_tag="MB216_000655"
     CDS             complement(19026..19361)
                     /locus_tag="MB216_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525513.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(19375..20409)
                     /locus_tag="MB216_000656"
     CDS             complement(19375..20409)
                     /locus_tag="MB216_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBT4525514.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(20499..21179)
                     /locus_tag="MB216_000657"
     CDS             complement(20499..21179)
                     /locus_tag="MB216_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBT4525515.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(21349..22308)
                     /locus_tag="MB216_000658"
     CDS             complement(21349..22308)
                     /locus_tag="MB216_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBT4525516.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            complement(22529..23632)
                     /locus_tag="MB216_000659"
     CDS             complement(22529..23632)
                     /locus_tag="MB216_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBT4525517.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(23854..24324)
                     /locus_tag="MB216_000660"
     CDS             complement(23854..24324)
                     /locus_tag="MB216_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBT4525518.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
BASE COUNT         7015 a         5172 c         4207 g         8293 t
ORIGIN      
        1 ataaataaaa taggtctata caaaaagaag ctgggaacaa tagatgtcca gcttccttct
       61 gtattttagg attgttttat gctttttttc gttcattcct cataattgaa atcgattgtt
      121 tcaatgaact ccataatccg ccttcgctat aaacgagtac attcgatttg tacatgtttg
      181 cagacaagat catcaataag aatgttacgg ctacaagtac caacaaggaa atcaatgctc
      241 cacccatacc gactgtctca tttgctaagc gaatcggcat aatatatgaa gataagaatg
      301 gaatatacga agtgatccga ataacgatat tgtttggatc tgaagctcct agtatcaatc
      361 caagcatgta tccgcctaga gatagataag tcactggcaa aattgctttt gctgtatctt
      421 ctgctttgtt gaccaaagag ccacataaag ctgctaaaac agaaaaaatc aaaatgccaa
      481 agatcatgaa tagaagagaa taccacaaga atggtccaat caagttatcc aatgagatac
      541 cgtctaatat atttttgacc atttccatat ctttgaattg actgtatcct ataccaaaaa
      601 taataccgta taatgccatt tgcgttaatg caacaagtaa aacacctgtt aattttccgt
      661 aaaaatgctt ttgtgcagtc gtactggaca ggatcacttc catgatccgc gtgccttttt
      721 cagaagcgat ctcttgtgca atgatctgcg catacgtcaa aataataatg aacaagacaa
      781 tcgttgctac ataacttacg acgtattgga ctgtcgagtt atcttctccg actgtcattt
      841 ttccttttga atcaaaattg actttttgtc gagtcaaact tgcttgttgg ctcaagctag
      901 ctacttcttc tggtgaaatg cctaattgac tcgctcgaag catcgattgc aagccagtca
      961 gttgttgttg tatcagtagt tgtgtacttt gtcctaatga attttcgcta taaagcatcc
     1021 catttatctt atcattctca gttgagacta ctaaatacgc atcgattttt tcttccgaaa
     1081 gctctttttg tgctttttct tcagaatcaa ctgctttgaa ctcaaaatca tctgttttga
     1141 cttggctcat ttgctgactg atttgctcat tatcagccaa tacgccgatt ttattgacat
     1201 cactattttg ttgagcaaag tttcctgcta cataaatgat acccagcatg ataaaaggga
     1261 ctaatatcat gatcaagaaa gaaatcgact taacattttt taaatagaca tctttggtaa
     1321 taatccaaaa cttacgcatc gatttcacct gctttcaatt tgaaaatctc ttccaaagtt
     1381 ggcggttgct ggttaaacat aggaatgtag ccgaactgag tcgcacgagt aaagatttct
     1441 ttcccagctt caggatcttc taaggtaatc tctaacgtat tgtcttcatg aaggatcact
     1501 tttttcacgc cttcgatttc ttctacttcc tgttggctta aaccagattc caagaataac
     1561 ttcgtacggc caaatgattc acgaatctca tgtacttttc cattcaaaac catttttccg
     1621 tttcgcaaca tgatcaaatg atcgcagatt ttttctacat tatccatatt gtggctagaa
     1681 aaaatgacac atgaaccatg ttttttcaat tccaatattc catcttttag caattcagca
     1741 tttaccggat ctaacccgct gaatggctca tccaaaatga tcaatttagg ttcatggatt
     1801 aacgtggcga tcagctggac tttttgctga tttcctttag acaaagattt tactttatcc
     1861 gtctttttcc cttttacctg aaatttttcc atccaaaagt cgatttttgg ttcaatctct
     1921 tttcttgatt tccctctaag ttctgcaaaa taaagcagtt ggtcttgaat cgttactttc
     1981 ggataaagtc cccgttcctc cggaagataa ccgatgacat cataatcttt ctccccaaga
     2041 tgatggccat tccataacac ttctccttgg tcttgtgtca aaaaatccaa gatcaaacga
     2101 aaagtcgttg tcttcccagc cccgttttgg ccaattagcc ccatgatttt tccatcgggg
     2161 atttggaaag agacatgatc gacagctgtg tagttcccaa aagttttaac aagatccttg
     2221 acttctaaca tttcttcact ccttcttcaa gaaatcacat atatccttag acaccagacg
     2281 taagcgattt attatttacc aacaaaacgt aaacgctctt tttgttgtac ataaaatgat
     2341 aggacacttt actttctttg taaagtgccc tttcctcatt cctgatgtaa tttatcaaat
     2401 acgttttgtg cgacattttt cggcagtccg atttcttgta attcttctgt cgtggctgct
     2461 gtgatatttt tcaacgattt gaactcttta agcagcattt tttttcgttt tggtcctaac
     2521 ccttcaattt catctaatcg ggaagcaaaa ctgtttttac tgcgtagctg ccgatggaaa
     2581 gtgattgcga atcgatggac ttcatcttgg attcgctgga gtaagaaaaa ctctggtgaa
     2641 tttcgctcaa gcggtactac agccaaatct gtaccgaata gcaactcatt tgttttatgt
     2701 ttatcgtttt ttaccatgcc agcaatagga atatcgattc ctaattgatt ttctaagaca
     2761 tcttttgcca catctacttg tccttttcct ccatcgatca agatcaaatc aggtaaaggc
     2821 aacccttcgc gaatcactcg cgaatatctg cgatagatga cttctctcat tgatgcataa
     2881 tcatccggtc cgttgactgt ttttatttta tatttccgat attcttttct tgccggtttt
     2941 ccatcaatga atacgaccat cgcagccaca ggattcgtcc ccatgatatt agagttatca
     3001 aaagactcga tacgtattgg ggtcgggata ttcatcgcgt ttcctaaacg ttcaactgct
     3061 ccgatcgtcc gttcctgttt tctggcaatc agatcaaacc gttcattcaa agcaactgag
     3121 gcattcttac tcgccaattc aactaatttt ttcttttctc ctcgttttgg ttgcagcact
     3181 ttcgtagcta acaaagcttc tacactgggc ttgtcgatcg tatcaggaat caagacttct
     3241 ttaggaatga aatgctcatt ttcctgataa aactgaccga ggtaagtcaa gaaatcttct
     3301 tctgcttcgt tatagaaagg aaacattgaa acatctcgtt caattagctt tccttgtctt
     3361 acgaaaaaca cttggacaca catccatcct ttatcaatcg cataaccaaa tacatctcga
     3421 tccagtaaat cagtatttgt cattttttgg cgagtcatga ttgtctcaat ggcacggatc
     3481 tggtctcggt attctgcagc tttttcaaac tccatgtttt cagcagcttt ttccattttc
     3541 tgatggatct ctgactcgat tttttcgtgt cctccgttta aaaagttttt cacttcatca
     3601 acgattttcg tatagacttt cggatctacg tcgaaataat aaggacacag gcattgtccc
     3661 aaatgatagt aaagacaagg ctccttcgtc tgactaggtt tgcattttct cagaggaaat
     3721 aatcgatcga ggatcttctt tgtctcattc gccgcaccga catcgggata tggtccaaaa
     3781 tacagtgctt tgtcttttag taccttacga gtaatcacca aacggggata tttttcattc
     3841 gttatcttaa ggaaaggata ggttttatca tcctttaaca taatgttgta tttaggatca
     3901 tttttcttta tcagattgat ctctaagagc aatgcttcga tattcgattc tgtaacgatg
     3961 tattcaaagt cttcgatttc actaacaagc cgttccgttt tcgtattatg gctaccagta
     4021 aaatatgagc ggacacgatt ttttaatatt ttggctttgc cgacataaat gatcgttcca
     4081 tttttatctt tcatcaagta acagccgggc tgatcaggaa gtaaagctaa tttgtttttg
     4141 attcgttcgt tcatctggta ctccccttca tattcaatta aagttattat agcatgtctg
     4201 tcaaaaaaac aatccagcct gacaacgtaa ttatactcaa aaaatattaa tttgaataac
     4261 cttaactcaa ccgcaaaaaa accgaggacg gaactggatt cagtcccacc ctcagcttat
     4321 ttttgctgta agtttttata aatattgatc gatcatttga cgtagttgtt cttttgaatg
     4381 aacaccaaca gctttttcta ctacttgacc atcttttttc aacattaatg ttggaatgct
     4441 catgataccg aattgttgag gagtttctgg attttcatcc acatccattt tagcaattct
     4501 gaattcttct tcatcatatt cttgttccaa ctgatccaaa atcggtgctt gcatacgaca
     4561 tggaccacac caagttgccc aaaaatcaat caatacaagt cctttatctg tttcttcatt
     4621 aaatgtctta tctgtaatta cttgtgacat atcaaagacc tccattttta aaatctatga
     4681 ctagtatagc atttctatac ttggatagcg aaccatctgc ttacctttag taagctttac
     4741 ttaaatttaa caattgtcgc accatttccg ccttggtttg ccggtgcaaa ctcaaagctt
     4801 ttgacgctcc gatggttttt aagataatca gtaattccct tgcgaagtgc gcctgttcct
     4861 tttccgtgga caatagtcac ttgaggataa ccagctagaa tggcagaatc caaatattga
     4921 tctacctcac ttagcgcttc ttcgtatcgt tttcctcgaa ggtccaattg attcggcacg
     4981 tgactactag attcagatcg tacagcatgg ataactcgtt gtgtcggctc tttttgagga
     5041 gcaacaggcg tcagctcgtc ttcggacaca ttcattttta atataccgat ttctacttgc
     5101 cactgattcc cgtttttacg taaaagcgtc cctctttggc cgtaagtcgt aaccaacact
     5161 tcgtctcccg gtttcaacgt tttttgttct ttcgccttct tcaatacttt atttttcttc
     5221 aaagtttctt cttgatgtag atttgctaat tgggtctttg catcgatcaa ctgatgctct
     5281 ttcacatttc cttgcccaat ctgctgttgc attttacgaa tatcagcaat gattttttct
     5341 gctttttctt ccgcctctga cacgacttca tttgcttttt tctttgcttt ttccatttct
     5401 ttttcacgtt cttcaaagaa atagctgtaa gcttctttca aatcatcgtg aagttcttgt
     5461 gcctcagaaa caaaatgacg catttccaga tattcggtct ctgccatttt ccggcgattc
     5521 tctaaatctg tgatcatttc gtttaaatct tggctctcat cattcatcaa ctgcttcgct
     5581 tcatcaataa cttctgtatc aagacctaat cggctagaga tttcaaacgc attgcttcgt
     5641 ccaggaacgc caatcagcaa acgatacgtt ggacttaatg tatccacatc aaactccatg
     5701 ctggcattaa tcgtatttgc ccgattatat ccataaactt tcaattcagg ataatgcgtt
     5761 gttgccatca cataagcaga ctttttacca agatcatcta agattgcgat tgctagagca
     5821 gcaccttctt gcggatctgt tcctgcaccg agctcgtcaa ataataccag acttttttca
     5881 ttgacttttg ataaaatatc tacagtgttc gtcatatgag aagaaaaggt cgatagactt
     5941 tgctcaatcg actgttcgtc tcctatatcc gcaaatactt cctcaaaaat accgatttga
     6001 ctttcttcac ctgcaggaat cggtaatcct gcttgcccca tcagttgtaa cagacctaaa
     6061 gtcttcaacg tgatcgtttt cccacctgtg ttaggtcctg taatgacgat ggcttgatag
     6121 tcttttccga ttgtgatgtc attagaaact actttttcct gatcgatcag tggatgtctt
     6181 gcttgtttca agacgacatg attgtcttca ctgattcccg gcacgatggc ttttacttct
     6241 ttaccaaatc gtgcttttgc attcatcaga tccattttac caatcacata agtattgtgg
     6301 atgatttctt gacgatgggg aacaagttca gcagaaagtt cacttaaaat acgttgaatc
     6361 tcatttcgtt cagcgatttg gtattgacgc aaacgattat tcaaatccac cacttgtttt
     6421 ggttcaataa ataaagtctg acctgaagca ctttgatcgt ggactacacc gccaaataca
     6481 ccgcggtatt cttgtttgac tgggataacg tagcggtcat ttcgcatggt gatgatcgca
     6541 tcacttaaat actttgcatt tttcccacgg acgatcccat ctaactgttc acgaacagct
     6601 tgttcacttc gccgaatgtt ctgtcttatg ctctttaact caggagaagc gtcatcagtc
     6661 acgcgtccgt cttcatcgat tgcttccttt aaacggcggc tcaaaacagg gatcgtgact
     6721 aactgatctg cccacatata caaacgtgca aattcgattt cactgtcaga aagatcatca
     6781 atgaaacgat tcaattcgct tgtcgtagac aatactctgg aaacttgtgc aagctccact
     6841 ccattcaagt ctgctccgat ctcaatccgt ttcatatggg gacggatatt ttcaatttta
     6901 ggtataggga tcccgcctcg cagacgttgc actttcaatc catcctcagt ttcttgaagc
     6961 cagtttgtga tcgtttgttt ctcactcact ggtaccaatt ctgccagttc ttcttttcct
     7021 tgtgcagtca ctacaaattg tctgaccatc tgtttgactt tttcaaattc caatgtttca
     7081 aggattcggt tattcatttt atcaccttat ctttcactat gaaaaaagag ctgtgccgga
     7141 catacgctac gcagccttct taaataaact actctgtaca aaaaacgaaa tattttccag
     7201 cactattttc cttaattcaa aatattcgtt acccaaagct catacatctt gttggtaaga
     7261 aatggcgttc tctcaacgat aaaacgagcg aaactatgct cgccgaattg attttggacc
     7321 acatctaatg gaacaaaggc aagtaactgc aaaatcaaaa atatagtaac gtaagccatg
     7381 attagtgaca ggatgcctcc tgcaatccag tctacctgtc tcaagatcgg cacatacgtc
     7441 aatccatgga caaaaacccc cacaaatcgg gtaagcagcc agccaattag caaaataatc
     7501 agaaaagcag ttcctgcata aaaagcttca tctaggtgaa aggcaaatac ctgatcaaaa
     7561 aatgctaact ttgatgttgg ggtgacagct gggtatggaa tgtataattc caaatgactt
     7621 gctaatggtt tgtaaaaatg ctgggcagca ataaatgaca atacatatcc aatcgcgaat
     7681 atgatttgca aagcaaaccc gcgacgtgct ccagaaaaaa acgcaattag caataaaaat
     7741 aaaatcagca aactcagcat gctacgttcc tttctcttct aactttttta aatttttctt
     7801 attttgataa aaggatctta attagaagat ttttgtttaa tttgtccttt tcgtttctcg
     7861 tttaaaatct gttgtgcttc cacatgattt ttaatggtat gatctgcttg accattttct
     7921 ttcaatactt cccgagcttc gttctcgatt gcttcaatgc gtttcacacg attttcaagc
     7981 tctgtaaatt tgatcatttt tttctttaat tctgctgttt cttcttccag ctccaatatt
     8041 ctttcttgtt tcttcaattg atccgaaagc gcattgacag ccatcaagat tgctgcttgt
     8101 tcattatcca tttgtggtga taattgtttc aattctgcta actgttcatt gatcagcttc
     8161 gttacgatat ccatatgatg cttggtttcc cgtccgataa tagtatatgt ttggttggca
     8221 atcaccgctt tgtagcgtgt tttttcgtgc gccatacgaa tcatttcctc ctatttgatg
     8281 aaagttttat ttagtgtacc atgaagttgg ttctttcacc gttctccaca aatatttttc
     8341 acatacttaa ctattatatg ataaacttta aaaaaatgct atgtctcgca atctcttaga
     8401 aaacgaaaag tcgtgagttg cggtatagtt ttatgtataa aaacgaggag aattttatga
     8461 gcaacgtcat tatcaaagca acaaacgacc aacttagtca aatgaaagca tactatcgct
     8521 cttatttgtt aaataaacag attccttata ctagctttgc tgcaaaaaaa gacgggacga
     8581 ccattaccgc ttatacatcc ggaaaagtga tgtttcaagg tacgaatgca gcaatagaag
     8641 ctgataaatg gggggaggca caggataaac aagccaagcc agcaaaaaca gcctcttctt
     8701 tgccaaaaaa tatcagtcaa ttatccgttt taggtagtga tgaggtcgga aatggcagct
     8761 atttcggtcc gctaacagta tgtgctgctt atgtgaaacg tgatcagctt gcagaattac
     8821 gtaaacttgg tgttagagat tcaaaagagt taaaagatag ccaaatcatc cagcttgctg
     8881 acatcttaaa aaaaaccatc ccctataaac tgcttgtctt atctccagaa aaatacaatg
     8941 agatccaacc aaaatacaat gccgttcgga tgaaagtcgc tttgcacaat caagcgatcc
     9001 atttgctgct aaaaaaaatc gagccagaaa aaccagaagc gattttgatc gatcagttta
     9061 cgccggaagc aaattttaaa aaatatgcgc gtttagaaaa aaatcaatta caagaaaaac
     9121 tgtacttcgt tactaaagga gaacaatatc atttagctgt tgctgctgct tctatcatca
     9181 gtcgtgccag ctttttggaa gagttagaca aagcatcagc tgaagctgga atcacccttc
     9241 cgtcaggagc tggaacgaaa tctgaccaag tagcggcaaa actattggag aaaggcggca
     9301 tgcctatgct tgaaaaatat gccaaacttc attttgccaa tacagaaaaa gctttgaagc
     9361 tactaaaaaa ataatggaac tgtgacaaaa gtcatgtcac agtcccttgt tccgaataaa
     9421 cggtgttcaa tcatctgatc ctcggtatta gctcaacaaa cggcaaaata tgaggaacta
     9481 tttttccgtt tgtttctcta atactcagaa caaaacgttg atttcacaac ctttttctta
     9541 aacggtgttc tcatgccaac tcctcggtca caagtcacaa tagtcatcaa acatgagaag
     9601 ctgttttttg aaattgctcc ttgtgactca gagttgaacg gcaggctcac gacctcttcc
     9661 ttaaacggtg tttaaaagct tgcatctgcg ataataagcc cggccaaacg aaaaatatga
     9721 ggagctattt ttgtttggct gggcttattt attactgaaa ataagtggtc aatgcttgtg
     9781 tcaaacttgc agcaatctgc tgctgataac tgtctgttac taattccttt aaatcactat
     9841 cattattcat ataacccatc tcgattaaaa tagaaggttg aagattttct cgaagaactt
     9901 gatagttccc agtggagatt cctcttgaat ccagaggcag atcagtgaag gagtcagcaa
     9961 ttgtctgtgc taatgtttcc tcttgtccat agtaataata tgtcgtgatt ccagttgctt
    10021 cattcgcata ttccgtagag tctgcgtgta aactgataaa gacatctgcc tttttttctt
    10081 cactgtagtc gcatatatct ccaagctgta ctaattcatc tgtagtatgg gtcaaataaa
    10141 ccgttgctcc agcatcctct agcgcctttt ttacttttat ggctgtactt aatgttatat
    10201 ctttttcttc tgtttgttca tcattgctta atgccccagt atcatttccg ccatgcccag
    10261 gatctaatac aatcactgca tcagatagct cttccggttt tgtttgtata ttctttgtga
    10321 ctttggttcc atcttcgagg gctaaatatc cagtatagcc atcatttgtc ttgatctgat
    10381 aagcattttt tgtttcagaa agaaactccg ccagctctcc gccgtttata gttgcaagtg
    10441 gggtagttag cgagtcatct gtatatagtg tacggtcatt ttttccgatt ttgattaatg
    10501 tctcttgttc tgtatcatca aatgattcct ttgtctttgt aaccgtactg ttcgtactgc
    10561 tgaccgtttg tccaactatc tttttttgac caaaaacatc tatttgaaaa cagataataa
    10621 tgataatgat tgcaaataca aaagctccaa ttattttttt cattcttgac tctcacctcg
    10681 tagatccaga ctaagggcca aaacttaaaa tcagcttaag taagcaaaaa aggggctgta
    10741 aaagatataa ccgtagaaaa taacattact gctaaaatga tttttttcat ttttggcata
    10801 tcctgaaatc tccttttgac tattcgtggt gattcatcta cccaataaat agtttccttc
    10861 attctacaaa caattaattt cttcgttatt aaattaaaac acttcctgga acaccgacat
    10921 aataaataaa aatataccct gataatcatg agagaataac aaatttgtat ttatgaaaaa
    10981 ctacctatat aaagaagcaa gatataacga tcttcgtcat atcttgcttc ttcacctaac
    11041 tattttctcc ctatataaca gctttacgct tctcctaata caccattcaa atataaatcg
    11101 atcaattgcg ccacatttag cggttcttcc acaggtcttt tcgaccgagt gatgtaaggt
    11161 gctaacacta agaaaaaatg ccttgctgcg atttcttttg aaatcccatt tcttagcaca
    11221 gattggtttg ctgaaaagat ttcttcaaaa ggttcacgat aatttttctt ccatagaaag
    11281 aaaagatgat ctcttgtttc ctttgaaaga ttgctttcaa gatcgtgaag catcagaaac
    11341 aaatcgacca gatgtgtctc aatcaaaaac tgcgccattt ttgtcaaggt tacttgaaat
    11401 cccgtttcgc ttgtttgaac gatttctctc aaagcttttc caacatcttt taagtggttt
    11461 tctaaaactg ccgtatatag tttttcttta tcaccgaaat aatgatagag attgggctgg
    11521 gtgatcccaa ctttttgtgc gatttctctt gttgaagtgc cgagatatcc cttggacata
    11581 aacaagtcag tcgcttcttt caaaattttt tgctgtgtat ctttgttgta tcgatggacc
    11641 atttttccac ctgctttttg gttattcttt ttctttcaat acaccatctt ccatcataaa
    11701 tattctatcg catttgtcaa tcagccgtgt atcgtgagta accatgatcg ttgctttatt
    11761 tttgtctttt gattcttttg ccaagatatc gactacttct cgtgcctttt ctgaatccaa
    11821 actagcagtt ggttcatctg ctagaataat ggacggatca ttgtacaatg cacgagcgat
    11881 tgcgaccctt tgccgttctc caccagatag ttcttctgga tacttgttga tcaacttatc
    11941 cacgccaagc tgctcaaata attcgtttgc ttctttttga cgatgttcat gtttaatctt
    12001 atctactaat aataactgct tttttactgt cagaaaagga accagattgg aagcttgcaa
    12061 aataaagcca atttctttaa aacgtagttt aacgcgttcg ctttcttttt gattactgaa
    12121 ttcttgatca ttgataaata cagagccact gctaggcgtc tgcaatccgc ctgccaaagt
    12181 caaaaaagta cttttacctg aaccagaagg accgatgatt gcaacaaatt cccctttatc
    12241 gatactcatg gttgtttctt tcaatgcttt aactacttgg tcgccatctt ggaaactttt
    12301 ttctacttct ttaaattcta ttgctttcat gttcttcctc cttcttatcc aattgccttc
    12361 aacggatcga tttttacgat tgtccgaaca gaaaatagtg cgcctaacac agctactact
    12421 aacatcaaca agctaatgac gccaaaaaac agccaattgc tttggaatgg aaccgcatca
    12481 ggtagaacaa gggatgttcc cactgttcct aatagaccaa gaagaatacc aacaaatgat
    12541 aaaatgaatg tttgtgccac tacagaacgc gcaataaatc ctcctgtgat cccttgtgct
    12601 ttcattacac caaagatatt aatcttttgc atcgtcagta cataaatgaa aatcccgatc
    12661 acaatagccg caataacgat caaaaatccg atcataaatc caaaagtcaa tacttgcgca
    12721 ttatatccag gtaattcatt gatgaattta gaaattttga tctgttctaa atcttcaggt
    12781 aaattactga ttttacctct gacaactatt gcgttaatcc gtgcattttc acttgtatct
    12841 tctttttcga agcggatttc ttggtaagca ttgatggttg tatagagaac tggtgagaca
    12901 ttgaatttgg catgatccgt aaagccagtt agtttaaacg tcttgtcact accagataac
    12961 ttaactgtat ctcctatggc tagaccatat tcttctttca aactaatatc gcctactgcc
    13021 tcatcatcgt tatcaaaagc ttttccttca acaagattgg gcatgatgaa ttgattttta
    13081 tcgataccga agaagctaac attgattttt ccttcttctg tactgtcttt actagtcacg
    13141 actccaggtg tttgggcaag atacgccact ttcccacctt cgacttcttc tgctatcttc
    13201 ttagtaatca tggacatccc tagatttgaa tttgcatctt tcgaaagcac aatcgaatct
    13261 gcctcccact tgtcgactgc tgttcgatta tcttgagcta agccataagc taacccagtc
    13321 aaaaaaaata ccaggtaggc gatcaaaaac atcacccctg caactaacgc ataacgtaac
    13381 tttgaatgca taatttcatt taatgctaaa aacatttaac tcctcctcct gaaataatca
    13441 ttttatacaa caattttatc acttgataaa tttttggcga atattatgct caaaaaattc
    13501 ttccgctatt tcattatatg aattttttat tctcgattga ttaatattta ttatatcccc
    13561 acacaataaa agaccgtgat atttctcaca gtcttttatc tactctattg ctatttgctc
    13621 tcttagctag tactttaaaa gctaactcat tgaggtagtg aaaacgagag gtgtattcag
    13681 acagaaatcc aaaatcctca actgctagct gtggaatcgt agttttgtgt ttcacgatat
    13741 ccgcaagcag gttcaaatct gataataaga attcttggct tatcagctgt ccgccaatta
    13801 tttctaatgt atccggatgg tagatcagct ttgcatttac tgttttgttt tcttcgtcgt
    13861 attgtggaaa tagatcaaat tctcctgaac acgaaccggt attttgttcc aagaatggcg
    13921 cttcatcttc tgtcagccct acagttccta aaaagtagtc tataatgact gctgctgtta
    13981 tcttttgtct gaggtcgtaa gcggttggct gaccactcaa agttagcgct actgctcttg
    14041 ccatatgtac agctcgagta actaaaggta aaaagagatg ccgtttattt ttgtcaaaac
    14101 tgactgggac gatatctcca atcgcataga tgtccggatc acttgtctgc agatattcat
    14161 tgactatcac agtctcatcc tcatacaaag ccactttttc ttttaacaat gtcgtattag
    14221 gacgagtttg ggcagaaaaa atgaccatat ctgcctttat tgtttgagac agaagttgta
    14281 cggatactac tttctcttct gattttttcc aatcgatcaa gaattcattt ggataaaaat
    14341 ttatttgctt agtttgcagc tgattttgaa aactgttgat catatcctta tcgaaatatc
    14401 ttgataaaac attcggcata taatcaacta gatgaataat gtatccttta tttctcaaag
    14461 catctgctaa ttccaatcca atgtaaccag caccgaagat cactacttct tttgcttctt
    14521 ttaaagcggc aagtacttgc ttagattgca ctagattttt ataggtgtag acattttcag
    14581 cttcaatttg tttatacaag gtgacaggag aggaacctgt cgctacgatt aatttatcat
    14641 aggttatggt ctcgtttttt ccttctttgg atgcgaggat gagctgatgt ttgtctggat
    14701 caagttcagt tactctccat tcggtgatca gttttgcccc actttcttga agagtccgca
    14761 tgttatgggc aacttcagct aaactacgta tctttccttt taaataaagg ttgattccat
    14821 ttgaaacata gcctagtcca tctttatgat cttcatcgat cagtaaaact tctgctttcg
    14881 gttgaagttt tcttagattt agggcagctg tgatgccttc atgggaagca ccaatgatca
    14941 cgattttcat gttttattca tccttaaatt caatttttct aaataacatt cttttaaaaa
    15001 ttggttcaaa taggggaaat cataattata ttcgttgcta gtgaatacaa aaactttagc
    15061 ggacttctgt gacgggtact ctttcaattg gctagtcaaa atgatatcat actgcttttc
    15121 tttctcgtat tcgactaaat catatttgat cgtattcagc aattgttcct ggatgatttc
    15181 tttcacgata tccgccatta gcctttcata aggaagatca taagcaattt gcagacgtaa
    15241 ttctaactgc ttgatcagcg tccgtaaaat agatgcatag cgagcaaata attcaaagcg
    15301 ggcattttcc tcgtctactt cagtaaaatc aacaatagca aatgtttgtc tgaccaatct
    15361 ttcacataaa accaggaatt gtttatcttc tacttcagtt aaccgctgtt ttaatccttc
    15421 ttggccaaaa atattcatcc atcctctaaa ataggcaaaa tgagcgtgat tttgcatcac
    15481 tgtcaacgca atcttttgcg aaatctcttc tggaaaaaca taaaaagaga aatattcttc
    15541 tatcgattgt ttaaacgttc gattcaattg agctactacg tcgtctttgt atttttcgct
    15601 tatcaaaaac tcctggacat gcgagttatg tgtatctaat gtaaagttac tgatcatgaa
    15661 aagataaaaa accaacgttt cattttgatt ccaaggaaag gcatattgaa agaagtaacg
    15721 ggtcaaaaat gtatggaggt ccaataaaaa ttgtgtcgtt tgaaacaatc caacaggaag
    15781 attgctataa tctggtttgg attttttcgt acagcgcatt cgcttcttca taatggaaag
    15841 ccatatttca aatttcaatc gtccttcttc tgtaatagca aaactaaact ctttttgcag
    15901 cagatctaaa aaagtgtttc ctgataaatc actgtattct tttttgtttt caagataggg
    15961 acgtccaaac caaaataatt ggaaataaaa ataacgaatt tgactttctg gaccggtcag
    16021 ccgcccgctt tttattttca gatgaaactc atttaaagaa tcatttaatt ccttcactct
    16081 gcgagacaaa gtagccgaac tgatattaag ttccatgaca agtttctgcc gatcaaatga
    16141 gccattttcc aacagataaa gaagtatctg atattttgta gaatagcgca agtaacaatg
    16201 ataataaaca tcgactgctg ctatatttcc atgtctgatt aataaaatat tcccttcttg
    16261 gataacaatt tccaattgtt tgatattttc caagttagct tgtaaataaa acagatcttc
    16321 tttcaaggta gcccgatcaa ctgataagcg ggtaagtacg ttattaacgg aaacctctcc
    16381 tttttcctct aataaaagtt ggaaaagttt gatttgccgt tgttcatttt tttcaagcaa
    16441 actgaaaagt tccaagtcca ctccttcaag catttctgat tttaacagca gaagtttcct
    16501 tctcatcctt gaaaaagagg atctgtgaca gttatcacag atccatacaa attctaatca
    16561 cttagctggt tggcccagct gtcagttccc atgtgaccat gccttgatac gttgcgccag
    16621 gttgagcatt cgtactgcta tttacctcta agagtaaacc atcggtatcg ggaaagtcaa
    16681 cgagaacctc tcctttagct gttccatcac tctcataaat caacgtgttt tgaccttgct
    16741 gaatcaattg ctttgatcca gaatgattga agtataattt tgttccgctt aattcttgac
    16801 cttgatcatt cttaaataaa gaagtcaatg tacctgtcag ttgccaagga gacgtgcttg
    16861 gaagccgagt atcccgcaca tcaaaagaaa aatcgccttt tgattcataa taacttgtct
    16921 tcaacggttt ggggagatcg ttaccaaatg taaaatcatc tggtacatta actaatgtta
    16981 attcttgttt ggggacaaga attgttttag cagatgtttg gacaactggg ttaggaagct
    17041 tagcaccatc tgctaatgta atattgctgg aaagtacagc tgcctttgtc agtatgctga
    17101 cggattgtaa agaagtatct acagtattta attgatagtt gtaagtaaga tgaatggtat
    17161 ctcctatttt gctcaataga tctgctggga cagaaactcc ttgttgaatg ccggtatctg
    17221 cgatagttgt accatttaat tttaaggaac cattgatata agtagtattt tcaggaagtg
    17281 agatcgtgat ttttttgtcg gttatcgcat ccccagattt tgttaacgtg aatgtcacat
    17341 tttcttgaat cgtatctcct gatagagcag acaggggatc tcctgtctca ggttgtctgc
    17401 taaggttttt tagctgacta tctgcactca cagatggaaa attagccact gttatttgag
    17461 gaagagtggt gattccagct tgttcagact gccatttgta cgcaaaagac ggatacatgt
    17521 tagtaagtac atcgtccacc aagcgataat caaaataata ttgccctgaa gccaatgcag
    17581 gtagtgtaga agtagctgta tttacacccc cgttatttga ttggatcgtt tggaaaagtt
    17641 gatctgtcca tcctgtcgct aatttttggg ttcctttcga ataaagcctg taatggatcg
    17701 aacccttttt tagttcagtt gctgaccaat tggccgtgac ttggtacgat gcagaagaaa
    17761 ggagctttcc ccactgacta tcggctgtta agtttgttgg taaaaccgtt ccgtctagct
    17821 tgtactgctc caataccttt aaagcaacag gagaaagcaa tccgccttgc gcatctttcc
    17881 cactgtacac gatattggct gtctgattca atccacttgt gcttgctgga gtatgagtca
    17941 gtattgctcc ttgtgtaacg ttttttcctg taaaatagtt gactacgcga tcaaagcgac
    18001 taaatggcaa ctgatcaaaa cgtccttcag tcagccccat cgtcaatgtc cgactattgg
    18061 tagagcctgg aaggttctgg ttatttccat ccacgtattc tgtggtatca gcaaatgtat
    18121 tatattttcc tgttgaatca cttggtgaga tgtaactatt ccacgtattt atctctgcac
    18181 ttgcagaaaa tgcatatgct cctggattca agtttccgcc agttaattta ggataaaatc
    18241 tacctgcagc tggagctaaa atcgaattga acatacgatc ttggataata ttattcgaat
    18301 cataaaaatc aacagaaaaa acggcctttt tagacataga aggttgtaat ttacccttgg
    18361 tcaaaatttt gtttggactc gtatcatatt tatctccgtt ttctgtcatc ttcgcgacat
    18421 tagctgtaac atcaaattga agcgggatat cgtccacgtt gaattcgcct cgcgtaataa
    18481 tagcaataat atctgtcaat agagtatttg tatcccacga ccccatccca aaacgcaagg
    18541 tatggcttcc tgctgataat aaacttccgc tagtaacctt gatgggggca ccggcaatat
    18601 acaagtaggc gttgttccag tcaattgctt ttaatacatc actgcttgat acatgttccg
    18661 gcaaattgat gatcgccaca aatgcaaatc cttgtgcagt gatattcacc aaatcaagta
    18721 catttgttag tttcacatgg ctttcccaat gaaaacggac cgtgccacct ttccaaggat
    18781 catttgaacc atcgttcaaa taagtagcag tcaatttagg caattggtta gccccagtta
    18841 gttggacaag cttcccgcca ttgactgcac tacgtccacc atttggaggt acgatatttt
    18901 catacgtgta tggatcataa gaaatggcag gaggcgttgc tccaacagct tctgccgaag
    18961 gtgaaaagat aaatatactt aaaacaaaaa gcgaagtaaa aaagaaggag cgaaaaaatg
    19021 aaaatttatt ttgtagcaac atgttcatcc tccttttcct ttcttctcca taacaacaac
    19081 cacgcaagaa gccataattc tataccaatg atcaaataaa gaaaacggat cttttgttca
    19141 ttcgtctccg ggaatacgcc atgttgatta gattctttat aaatcggtag ctgtgtctgc
    19201 tgttttgatg cgccattgac tgaaccactt ggatgcggaa ttggacttag atcataaaat
    19261 gtaatcgtac ctgaactttt ggtatctgct tggactggct cttgccagct gaagaacagc
    19321 aaacttgcaa taatccctcc caataagatc gatgttttca acgaaatcac ctcatcatgt
    19381 tgtttctcct tttttctttt tcttgtggac ccacaaaaac agcaaaatca accataacac
    19441 tagaactgca gcaaccaatc ctgcataaag ccatgggaaa gattgttctt gtactgcaaa
    19501 aacggaattt tcatttactt tttgtgcttt ttcttttttg atcgtaaagt tttgtgtaaa
    19561 tcgccattca ttactgtcgc ctgattttgc tatgatttct gcgcggtaat cgccggcttt
    19621 tgctttatca ccagctaaat caagattcaa gtgaaaattc gaatttggtg caactaaaaa
    19681 tgcattagac gtattttcaa tataaagctg attttcataa aagatcttgc tcgtaaatat
    19741 caaattgctg ataatcctag gctgaggatt ttggaaacga agctgaacag ttggctttgc
    19801 gttgacctcg tccaaatctc ctgataaaag tttcagatcc ggttctatcg tctcttgact
    19861 ttcttgtaaa acgactgcga gattataagc aatacgattt ttgatttgtg ctccttctgt
    19921 agcattttct gtttcagctt cttctttagg tgaaacatga atacccgcta atacacgtcc
    19981 cttaaagctt tttgctggga ttttcacttc tataggtaca gttgtttctg catgtgctga
    20041 tacattgatt tcctttttcg caatgagcaa aacgtcacgg acatcaaacg gcaatgtctc
    20101 atctaacgtt gggttttttt gactataatc gatcgttcca ccatcactcg ttaccgccgg
    20161 attgacagag acctcaaatg ttcgttcttc atctgaagaa ttgctgataa ttacttctag
    20221 tgtctgattt tgttccggtg ccactaataa atcaaaataa ccgattgctt tatctatttg
    20281 gttctctgga aataccggtt tgattccaaa gtctcctgct gaatcctttg cttggctgat
    20341 tgacggaaaa atgaaaaata gacttaaaat agaaaaaaac gaaacaaaca gtcgtttact
    20401 ttgattcatt gtccaccaca tcctttcaac aacaactgcg gtaaaaaatc cttcacctat
    20461 agtattttca ccgcagtttg ttgaatttat ttcatactct atgctactgg gccagccacg
    20521 aggttccaag tcaaatcagc cgtgaatgtt ccagctgctg ctttcggcac tttcagtgtg
    20581 atatttgctg gatcataaac agaaagccat gttcccagac cttgatcttt tgctgctgta
    20641 aatattggtg tcgctgcact tgctttactc aacccagtta cagtttgact agttggtcca
    20701 gtggattcgt tatctgacgt acttttaaca gtggatgttc caaaatctaa ttcagcgcct
    20761 tgcaaaactt gtgaaccgtt tacaaaatct gaaaccgaaa ctgtcacgtt ccacccttgt
    20821 gcttggccat ctgcacctac acctcgacga tctgatactt ggagataagg tgtatcgttt
    20881 tttgaagcgt ctacttgata cgtttttgtt ccgctttcaa tctcgtgtgt accaaatggc
    20941 aattcgggaa ctacatccag tgttaaagga cctgtctggc cagttgctgg attttccgga
    21001 tctgctggct ctactggttt tgaaggatca gtagggttaa gaggctccac ggtatttgtt
    21061 ggttcagtaa aggtaatatc tccttttgaa gtcgcttgac cctcagcaaa agctggtgtt
    21121 gccaagccgc ctagaagaag tgttgaaagt aataatgtcc caatcatgtt ctttttcata
    21181 ataaatcact cctttttctt agtgacctaa gtatagtaga gacagtatgc aaaaaatcct
    21241 catgaattca tccgcttact tgaaattatg accctttttg tataaaagag aaaaaaacaa
    21301 taaaatcgtt tacatgaata ttcttccttt tttattaagt ttcgtttgtc aaattaagtt
    21361 agacaaatca tcttttgtga cgtaactcaa aaatacttcc attggtgtcc gatagtgtaa
    21421 tgattttcta ggaatatgat ttcgtttcga tgccaccgat tggataaagg tttcagatac
    21481 accattaaaa tccatagctt tatacaaacc atctttacgt aataagccat ttgaatgttc
    21541 attcaatcct cgttgagaag gtgttcctgg atccgcaaaa taaattgaaa tatcgttggt
    21601 attactaatc tttttccaat ttgagaattc ttttccacaa tcaaatgtga tggatttaaa
    21661 taagtttttt gggacacaac gaaaccattg attcagccta ttttcaatgt ctttagcctg
    21721 tctgccttcg ggtttcaagg taatgatgac tttagatagc cgttcaacta atgtgatgac
    21781 cgcgctttta tgttttccac ccacgatagt gtcaccttcc aggtgaccaa attcatgatc
    21841 aaactgggta tagtctgtct ttctgtctcg gattgaccgg cgaaaccctt gttttcctct
    21901 tttttcttta tgaccgtttg gttttctttt tcccttcatt ggtaaatcgt gagtgtcaaa
    21961 ggttttatct ttaaacattc gatacagtgt gcgtaccgaa caagcaatag gaaactctgc
    22021 tcgaccaata aggacatcag gagcccaccc ttgaacaacc tttttttgaa tgtattcctg
    22081 ttgctcatca ggtaaaacaa tcggatgtct tccgcagtta cgtttgtttt tcttatattg
    22141 tttatagtag ctcattgcgc ttccaccaga tttaagaaaa cgatagacct tataaatagt
    22201 ttgtcgagaa cgttgtaaca gattggctac ttttgaaaca ggttgtgatt gattgaaata
    22261 agcttctatc attacgagtt cagttggtgt aagatggtta taggtcattt gtactcactc
    22321 cttataattc tttcgtcgga actattttga gtgtagcaca aatgactttt tttagttgtc
    22381 tagcttaatt ttacaatcgg cgaagtatga aaaaatattt caattttatt ttttcgtttc
    22441 aaattattct attagtgaca tccttgaact ataaaaatag gagtacggca tcatctgccg
    22501 cactcctatt tttttcgtca tttgtttctt aaataaaacg attctcacgc aattttattt
    22561 ttttgatatc ttctactgtt tgtgttccag ctaattgcat gaccatttcc aattctgttt
    22621 taaagaaatc aaacacttga tggactcctg tacttcctcc taaagacaat ccataaataa
    22681 cgggtctgcc aatagcaaca agatctgcac ctgatgcgat tgctttgaaa acatgctgtc
    22741 cgcgtcgtac accgctgtca aacacgattg gtacacgttt atctactgct tctgccacat
    22801 attgcaacga atcaaacgct gctggaccac catctagttg acggccacca tgattagata
    22861 cccagattcc gccagcacca gattctaatg aacgatagac atcttcttct gactggacac
    22921 ctttcacata gactggcaaa tcagaatgag cagcaataaa ttctacatct tttggactaa
    22981 gcttctgttt ggaagatttg tacacagcat ccatcgtttg gcctactcct gattgataag
    23041 cctgaacgat cggcattggc agtgggaagg taaacccatt tctgcggtct gtttcacggt
    23101 ttccgccaac tgttgcatcc gctgtcaata cgattgcttt tgctccatta cgcttggcca
    23161 cttcaagaat atccaaattg atcccatcat ctttgctcat ataaaattga aaccattgcg
    23221 gtgctttctc cccgcctgct tctcttatct cttctaatgt acaagaagcg taagaacttg
    23281 ctgtatagat cgtcccaaaa tctgctacac cttttgcaga agctttctcc gccttcacat
    23341 gagctaagcc atgagcagca actggtgcca tgatgattgg tgcagtcagc gtttctttgt
    23401 caaaatgtgt agttgtatcg ggtaattcta catctcttaa tacatgagga ataatcagct
    23461 gatgattgaa tgcccgttca ttttcttgat aagtaaataa gtcgccagca ccactgctga
    23521 tgtaaccata accaccagct ggaatcactt gtgaagcggc tatttctaaa tcttccatat
    23581 tgataaaatc cactattcct tcatttgtac ctgcttgata cgttttttcc atttcaatgc
    23641 cttccctcta taaaaaattg aatgaataat atgatagcgt ttacacactt tatattactc
    23701 ttccgtattg atacaggcaa ataatctgtt tacatttcag tcattccagt tgttactact
    23761 ctgaaagaaa tgtttagaac acaaatagat aaaaaacgat gggattttga caaaagtcgt
    23821 gtcaaagtcc cttttacgaa atactgtgga tgcctattga aataagtgcg ggttgtttct
    23881 ttgtatttca atcgtttctt gccattcttc gtttaatttt ggattaccgt tttcttcctt
    23941 gaacgcataa ggtcccaatc caaaaagctc agttagtatt tgattcacga ttccatatag
    24001 ctcggtgtga tcggacacat gattattgaa gatcatctca ttatcaccac gtttgaatct
    24061 cacattcggg ctctcatgat gaacctctat gcagttttcc ccttttttta actgacggat
    24121 ttctgaaaac atctcataaa tatcttctcc aataaaacct tcagcaagcg ccagactgtc
    24181 cacaagaccg cctactgtca actcagagat tatcgctttg ttggcaccaa gctttaatgt
    24241 atagcttgtt gcttctacta cgccgcatgc attgcaacca ccttctgata gttcgctttt
    24301 tctgatcaat tgaacttctt tcattttctg ttcctcctct tagttttcta cttatgtatt
    24361 ttttatcacc tgacgtaagt gtattttatc gtcacgatca cgtgatttat tcttcagaga
    24421 ttctacctga ataattcata cttttaatta aaaccatgtg aaactgcctc acggcagctt
    24481 acaattactt tttcatcttc acccgttaag tgatgaaaaa agaacccctt tctgctatgg
    24541 ttaaagtgac taaaccaacc aaaagaaagg agttcttttc atgactagct tacacaaaaa
    24601 ccaagtaaaa ttcaattcaa atatcactat ttctcataca ggtggtcaat tatcgagtga
    24661 ttcgggtctc gtcctagtga aagagct
//
LOCUS       DAGCYL010000020        24128 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.020, whole genome shotgun sequence.
ACCESSION   DAGCYL010000020 DAGCYL010000000
VERSION     DAGCYL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.020"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(118..267)
                     /locus_tag="MB216_000661"
     CDS             complement(118..267)
                     /locus_tag="MB216_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525519.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            1235..1561
                     /locus_tag="MB216_000662"
     CDS             1235..1561
                     /locus_tag="MB216_000662"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4525520.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1542..2477
                     /locus_tag="MB216_000663"
     CDS             1542..2477
                     /locus_tag="MB216_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBT4525521.1"
                     /translation="MQKWICDLFMCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSE
                     KNRALNCLTVSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEE
                     IQAAVEGAISVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGL
                     DTQPITAIAILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKP
                     LLVQIANALLRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSA
                     KGYLEPKPAKEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            3106..3357
                     /locus_tag="MB216_000664"
     CDS             3106..3357
                     /locus_tag="MB216_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HBT4525522.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3899..4057
                     /locus_tag="MB216_000665"
     CDS             3899..4057
                     /locus_tag="MB216_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4525523.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            4129..5355
                     /locus_tag="MB216_000666"
     CDS             4129..5355
                     /locus_tag="MB216_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4525524.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(5444..5836)
                     /gene="rpsI"
                     /locus_tag="MB216_000667"
     CDS             complement(5444..5836)
                     /gene="rpsI"
                     /locus_tag="MB216_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBT4525525.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5852..6295)
                     /gene="rplM"
                     /locus_tag="MB216_000668"
     CDS             complement(5852..6295)
                     /gene="rplM"
                     /locus_tag="MB216_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBT4525526.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(6814..7281)
                     /locus_tag="MB216_000669"
     CDS             complement(6814..7281)
                     /locus_tag="MB216_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBT4525527.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(7401..8939)
                     /locus_tag="MB216_000670"
     CDS             complement(7401..8939)
                     /locus_tag="MB216_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBT4525528.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9019..9705
                     /locus_tag="MB216_000671"
     CDS             9019..9705
                     /locus_tag="MB216_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBT4525529.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(9906..10433)
                     /gene="msrA"
                     /locus_tag="MB216_000672"
     CDS             complement(9906..10433)
                     /gene="msrA"
                     /locus_tag="MB216_000672"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBT4525530.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(10517..11194)
                     /gene="pgmB"
                     /locus_tag="MB216_000673"
     CDS             complement(10517..11194)
                     /gene="pgmB"
                     /locus_tag="MB216_000673"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBT4525531.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11191..12321)
                     /locus_tag="MB216_000674"
     CDS             complement(11191..12321)
                     /locus_tag="MB216_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBT4525532.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(12321..14504)
                     /locus_tag="MB216_000675"
     CDS             complement(12321..14504)
                     /locus_tag="MB216_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBT4525533.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(14501..15526)
                     /locus_tag="MB216_000676"
     CDS             complement(14501..15526)
                     /locus_tag="MB216_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4525534.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(15571..16398)
                     /locus_tag="MB216_000677"
     CDS             complement(15571..16398)
                     /locus_tag="MB216_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBT4525535.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(16395..17462)
                     /locus_tag="MB216_000678"
     CDS             complement(16395..17462)
                     /locus_tag="MB216_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBT4525536.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(17488..18324)
                     /locus_tag="MB216_000679"
     CDS             complement(17488..18324)
                     /locus_tag="MB216_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4525537.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(18321..19190)
                     /locus_tag="MB216_000680"
     CDS             complement(18321..19190)
                     /locus_tag="MB216_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4525538.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19260..20543)
                     /locus_tag="MB216_000681"
     CDS             complement(19260..20543)
                     /locus_tag="MB216_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBT4525539.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(20557..22242)
                     /locus_tag="MB216_000682"
     CDS             complement(20557..22242)
                     /locus_tag="MB216_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBT4525540.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            22517..23542
                     /locus_tag="MB216_000683"
     CDS             22517..23542
                     /locus_tag="MB216_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4525541.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         7030 a         5011 c         4320 g         7767 t
ORIGIN      
        1 aaaaataata caacttagtc attctttaca acgatttata gaaaaacaac ctctttaaat
       61 ataaaaggta cgggttactg attacttgta aatctttacg ttccagtttt attctttcta
      121 aaaaatatac tcacttcttt tcttagactt ctttttttga ttctcctctt tatggtcttg
      181 tccttttttg ccttcaaaat atttaaataa acccctttca aaagcatttc taggttttgg
      241 acaataagga atgacttttt tcttcatata aatacctcat ttaactattt gatatcatca
      301 cattatcaaa aaaaataggt tttttattaa aactatcttg acctttcggg agatttcact
      361 gtgatttatt tttattccca tacttttttc gttggtcact ttcgtatacc taatctaagc
      421 gatcatcggc gacggtgctt ttacaacgaa tggcaggaca aaacaagggg cggaaaaact
      481 tttttgccta tttcataaaa aactttttcc tacagcaatc ccttgttttg tcctaccaca
      541 cgttgtgaac ccaaagcacg ccgacgaacg cttagctata cgaaaatgac ctccaaaaac
      601 aaaaaagtga ggtgaataga aacaaaaaga aattgaaatc tcccaaaatg tcgtccgcct
      661 cggagacaac ttgctgctga aaatggttgg ttaattcatt taagtattaa ataagataaa
      721 tcatcaaatt aatcggaaaa atagcatgat tggccttatc aaaaataaac tgtaaaaact
      781 tatatttcta tgagtggtgg aacaatgcta ataccaaatt aatttatcga ttacccacct
      841 gcacaaacca aagatagcat tattaggaaa cgaatcaatc agcgaatcca gcattataat
      901 gaggatacta aaaataaagt tttcataatt ataaatccgc cttcctacga aaggcaccaa
      961 tgaggtgatg aaaaccttag agcgctttct ttcttttttt attccgtgtt accctttcct
     1021 tgcaggtccc tggtacacta cagaacccga ggaggtgagt ggcggggctg tatagcggca
     1081 accccgcata attatatggt gtcggtcatc cgttaaggca tcaatgaatg gcgcaaaccc
     1141 gtagcccgta aacttatctc ttccgaagtt gactcgattt cgccggatga ccagtcccta
     1201 ccaaacctgt actatttttt tacaggaggt tgctatgttc aaaatctttc gtaaaaattg
     1261 ttgtggcttg gatgtccaca aaacgtggat ctatgcgtgt atcggaatca cagatgcgaa
     1321 tggtcgtaca gtgtataagc aagctcgctt ctcttccttt acaaaaggtc tcaatgaatt
     1381 agctgaatgg cttgctaaat acaactgtaa ggatgtgtgt ttggaatctt ccggtaaatt
     1441 ttggattcct gtttttaata ttcttgaaaa aacttgtttt gtcacactgg ctcatccgaa
     1501 gtacaccaaa ccccagaaag gaaataaaac agatcgcaaa gatgcaaaaa tggatttgtg
     1561 atctctttat gtgcgacatg atcaaaccca gttttattcc tggtcttgaa atccgccagc
     1621 ttcgtgatct aatgcgttat cggacgaaac tgaccaacat gaaaacaagt gaaaagaatc
     1681 gtgctttaaa ctgcttaacc gtttctaatc tgaaactaga tgatgttttt agtgatgtgt
     1741 tcggaaaatc atccacttct atcatccaat acttgttaga acatcccggt gaatcatttg
     1801 atgttcgacc atttatccat gcccgttgta aaactcccgt tgaagaaatc caagcggcag
     1861 ttgagggggc gatttctgta cctcaagcga ttaagcttcg ccaatgcctc aaccatatta
     1921 acgagattga gaagcatcga gaagagatca atctagagat tcaagctctt gcagaaccct
     1981 tctcccctgt tttggaacta cttcgtacca taccgggctt agacactcag ccaataacag
     2041 cgattgcgat tctttcagaa ataggaccgg atatgtccgt gtttccaact tcgaaacact
     2101 ttatttcttg ggctggatgc tgtccaagaa acgatcgcag tgcccgacga acaaaatcaa
     2161 cacgtatctc tcgtgcaggg cagtcgttga aaccgctact tgtacagatt gccaatgctc
     2221 ttctcagatc caaagaacat ccagaattta aaaatcgcta tcgtagaatc aaagcgaatc
     2281 gtggacacaa gaaagctatc attgctattt gtaagatgct cctgaccgct atctggaatg
     2341 tcttatcaaa ggtggaaccc tattctgcta aaggatatct ggagccaaaa cctgcgaaag
     2401 aagagaaagt tctgacgcaa tcccaagctc tggacctttt aagaaaacgc ggatatacca
     2461 ttatagatag tgcttaaacc aaagttacgt tgattcttcc taaatagact cgttgtcttc
     2521 catttcaaaa attttgaaaa cggtaggctt atttagtgta ccgttcttca ggtatcttca
     2581 aatagattta ttttcaaact tatttccttt cttcttttac tctcctatag cttagcattt
     2641 aagtgggcac tcgtgtcacc ttgctagata gctatgaatg ccgtaataag cctcctagta
     2701 tataggcaga ctgccaatta taactgattc aatccacatt atgataaaat ccaccttttc
     2761 gtttcatatg tgttgaaata tcacatatgt caaaagtgca cgagtctttt cattcagtgc
     2821 tttgtaaata ctcaaaatta ttccttttca ctttaaaaaa aagataatta gtacaaaatc
     2881 agaaatgagg aaagtattag gatggactag aaaggagttg ttgaatttgt tgcaacagat
     2941 tggtcattta tcaattaaaa attaggtgaa gttttattca tcactgcagc tgctgtttcc
     3001 cagagaataa aacgtctaga agaaactggt cagttactgt attatcaagc agcaattgac
     3061 tacgaacaag cttgattact aaccaaagcg tttatccacc tgttgattaa atcaaatcaa
     3121 aaatcaggtt tttatacgtt cataaaagca atctccagtg tactcgaatg tgactgtatc
     3181 ataggtcctt attcgatgtt aatcaaaatg gggtttcctt ctaccgaatc attggacagt
     3241 tttatcaatg atttatcaaa gtttggacga accaacaccc aaatcgtttt ctcgactctt
     3301 gtttccttaa tggtttatca gttctggaat gtttatcatg cgcttttatt aaaatagaaa
     3361 agagcttttt tcatgcgaca aagcagtgat catttattgg cacatgcaat ctttgtggat
     3421 ccattttttg aaaatacgat atttagtttt gacccaaaaa tctttaaata cgtatttgat
     3481 aatttatctt aacaaccaga taattttatt cagcaccgta ttatctatat tgaattctcg
     3541 gaatcaagat aaattttttt ccgtttttgt ttctttttta atcttcttct tgttattaat
     3601 agtgattagg atgatattcc tatattgatc tttgataatt agatataatt tttgaataac
     3661 gttccctgct attcgagcta gaaatacaag tacgccatga cctagttagc gacaactact
     3721 tagagatttc ttttgttctg ggacaacaaa atgccatgac aatagcagta aataatgata
     3781 cttccgtaag gctcgccgta aactggggag aggtgaaatt cctatgtgct tacccaaagc
     3841 ggttcaaaaa ttttaaacta ttattttagg agatgatttt ataaaatcga aagcctactt
     3901 gaataaaaaa caagcctgtg aatttctagg aattgataat aatactttag ataagtggat
     3961 tcgtgtatat aattttccag taataaaaat tgatggcgta gttcgctttg acgtagagga
     4021 tatcaaagat ttcatgaata cattcaaaaa aaattgaaat tttcttctcg tcacatatac
     4081 tataaatagc gaaatgtact gccttacttt ttaaaggagt gaggaagttt gcctacaaag
     4141 ctttcaaatg ggaaatataa aactaatctg aggtatccaa aaaaattcag agaaatcact
     4201 ggaattgcat cagagaagta tcaaaaagta tttccaacta ggcaattagc tattaaggcc
     4261 gaaagggaaa tgaaaaagaa gattgaaaca gttttaagag aggagaatgc aaactcacta
     4321 gaattgaaag gaaaaataaa atttaaagat ttttatgaat caaaatggct tcctagatat
     4381 gaactaggtc aaactactag gtcaaacagg gctccaagta atgtaacaat tagtaatact
     4441 aaggatatat tccgattgca catccttcca atgtttggtg aatatgcaat gaattatctg
     4501 aattcaaata ctgagataat atcagatgaa ttaacaaaga aatcgaaaga atacgcaaat
     4561 ataaaaatta tcaaaggcta tgttcgctct atgtttgata ttgccgaaat actatgttat
     4621 atcgaattca accgaactac caaaattatt caatgtatta ccgcgcctaa gaaaatggct
     4681 ttagaagaaa aaaggataag ggaagggaat caagcattat catcagaaga gctgactgat
     4741 tggatagaag cagtaaacaa tgacttaaat aatcacttgc ttacacttca cgattacaca
     4801 cttttcatgc ttaccttata tctaggtgat agaaaaagtg agacttatgc tctgcaatgg
     4861 aaatatattg attttaataa aaatacggtt cgtctcaaat atgctttaga taaatatcaa
     4921 agaaagacac atacaaaagg ttggaaagac accgttattc aggcaccaga agttgtgatg
     4981 acattattaa gagaatggaa atcagctcag gctgaacaat tgttgaaact aaaaattata
     5041 caaacacctg accagtacct ctttacctat tccaaacctt ctggagaagt aaattgtcct
     5101 gttcacgctg actacttaaa ctatcgaatc aatgcaataa aaaaaaggca tcctgaattg
     5161 gctcaattat ctccacacaa acttcgtcat acctatgcaa ctatagctag ggaaggtgga
     5221 gcaaatatga atcaaatctc taatgcttta acgcactcgg atatttctac tacaaaaata
     5281 tacgtgaaca cacctgacgt agttgataaa tctgtttttg aagcatttca aaaaggattg
     5341 aataagtgtg attaacatgt gttctcaaat gtgttcttat tataaaaaaa gacactttcc
     5401 aaataattga aaagtgtcct gaaatattga tataaaaaga atattaacgt tttgagaatt
     5461 gtgaagcttt acgagctttt ttaagacctg gttttttacg ttcgaccata cgtgcgtcac
     5521 gagtaagtaa gccagcacgt ttaagagcta aacggaagtc aggatcaact tctagcaatg
     5581 cacgagcgat accatgacgg atagctcctg attgtccagc gtatcctccg ccgtttacgt
     5641 taacaaatac gtcatatgcg ccttttgttt cagtaacagc gaaaggttga ttgattactt
     5701 cacgcaagtc agcatgtgga atgtattctt caacatcttt tttatttaca gtaattttgc
     5761 cagttcctgg tactagacgt acgcgagcaa ctgcgttttt acgacggcct gtgccgatat
     5821 attgaacttg tgccaatgaa attccctcct attagattaa gtttgtgatg tctaaaactt
     5881 ctggttgttg tgctgcatgt ggatgttctg ctccaccgta tacgttcaat tttgtaaatt
     5941 gtttgcggcc taaagtgttt ttaggaagca taccttttac agaagtttcg atcaaacgac
     6001 gagaattttt agcacgcaat tcgccagcag tgattgattt caatcctcct gggtagttgc
     6061 tgtgacggta ataaattttg tcagtcgctt ttttacctgt taatttcact ttgtctgcat
     6121 tgatgacgat gacgaaatcg ccagtatcca aatgaggtgt gaaagttggt ttatttttac
     6181 cacgtagtac agatgcaaca actgttgaaa gacgtcccaa tggaacatca gttgcgtcta
     6241 ctacatacca tttacgttct acttcgcctg gtttggccat atatgttgta cgcacgttat
     6301 tttcctccaa tttaagttct gatgtttgac atacacttcg agtttccggg gctctttgtg
     6361 gggcaaacaa taccatttaa catattaccc gctaatctag tcaatgtcaa cgtttttctg
     6421 cttgattgaa aaatttatat gaaactttac ttcttttctt tcttttatat aaaagggtcg
     6481 tttcctgtca aaatgaaaaa caaaaagagg ctgtgacaaa agtcttgtca caacccctta
     6541 ttttgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat atccataaaa
     6601 cataagaagc tgttttttaa aattgtttct tgtgactcgg agctgaacgg caggcttaca
     6661 acctcttggt aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa
     6721 atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatttcac
     6781 aacctctttt tcggtcaagt tcaacaacag tttttacgcg tcgatctctg gcgctttgcc
     6841 caaatgagct tggatcatcc agatacgttt ttctgtggct gttttgaatt cgataaagat
     6901 atcttgtgtt ggatcgtcgc cttcttctcc agaaacttcg attccttttt ggtaaagatc
     6961 tgctagataa cgatagcctg caacaagtgt ttccaaacgt tctggaatag tacgatccca
     7021 gcggccaatt tcatcaggga tttttgtgtt atctgcaaat tcacgcaatg tagaataagg
     7081 tgaaccgtct aatgtgatca agcgttcgga tacttcatct aactgagcgt tcaaatcatc
     7141 catgaactca tccatcattg ggtgcaatgt aaggaaacct ggtcctctca tgtaccaatg
     7201 tgtttggtga acaacaacag acatttggct tagatctgca actaattgat ttaatacttc
     7261 tttggtttgt tggaatttca tctttcttgc ctcctaacat tttcttacac ttaaaataat
     7321 agtagacttt caatgaaaag tacaacaaaa tcatttacct gagaacgatt ttcagttcgc
     7381 ttttttatcc gtaaagccaa tcatttttca gaatcagcta attcttctcg cagttgagca
     7441 aattgctgat aagtctgtgc taaatgcttt tgtgtcgaaa caaataatgg atagattttc
     7501 tgatattttt tatgatcagt ttcttgtact ccaacttgct ctttcaattc tatcaattcc
     7561 tgtgcttcac tgatactttg gatctttccg atgcttctcc agcctaaaat cacagctcct
     7621 aatgcggatg cttctgtgac agtagggaaa acaagcgggt aacccagtac atcagccatc
     7681 atctgacgaa aaatcggaga ttgagcaaac ccgcctgtag cacggatctc tgtgagctga
     7741 ttttcctggc aaactccttt taaaatccga taaaggttga gacagatccc ttctagaatc
     7801 gccttcatca tttctttttc tgtatgttgc cgctgtaaac cgataaaact gccaaacgca
     7861 tctgcgtccc aaagcggtgc tcgctcacca agtaaatagg gatgaaaaat cagaccattg
     7921 gcacccgctg gcacaaaagc gatagccgaa agcagtgcat catatggatt ttctcctttt
     7981 tctttagcag attgaacgac ttcctgtaaa aatgtatgcc ctgcccaatc aaagatgcca
     8041 gcaccattgc tagtcgctcc tcccactacc caatgtttct gatccagaac ataacaaaac
     8101 gtctccattt ctggatggaa ctgaggctga gtagtaataa aacgaagtgc tccgctcgtt
     8161 cctacggtca aagcggcgat cgtatcacca aaagcaccta accctagatt agaaagcggc
     8221 ccgtctgccc ctcccaaaac aaatgatata tcatttggca aacctagttc tgctgctgtt
     8281 tttgatggta gacctgttac ttgataggtg gaaggaaaga cttgcggcaa ttgctctatt
     8341 cgaatgttca agtagtcaag cacttcttct gcccattgga attcatggat atcaaaatat
     8401 ccagttcctg aagctgttcc gtagtcgctg atcagttcgc caaacaatct ataaaagata
     8461 tagtctttga taccgacaaa ataagcagtt tttttgaata attcaggttg gctgttttgc
     8521 agccacttga ttttgttcaa tggtgtcatt ggatgaatcg gcgtcccagt ctgccgataa
     8581 aattctttgc caagcacggt atatttcagt ttttctgctt cgtccgctgc tcggttatct
     8641 gcccaagtga tcattcgaga taacggtgtt ttctcttcat ccaacaatat caggccatgc
     8701 atcgcagagg agaaggagac aaattgaatg attgtgtctg aaggcaactg tttaattact
     8761 tcttttataa cggtgcagac accttgaaag atcaattcct gatcttgttc tgccatccct
     8821 tgtgtttctt ggatcaaagg gtagcgaaaa taagcggatg ctgcttcttg ggcttcatct
     8881 gtgaaagcaa ctgctttggt ttgtgtggtt ccgatatcta atccgattga aacgttcatt
     8941 ttttcacctc aaattttatg tatgtacaaa aatttagttt aagcttgcgt atcttggttt
     9001 tgaaaagggg gagatggcgt gttactttat tttatcattg gttgctgtat cggatcattt
     9061 ttatgtttaa ccgcccagcg gattccgtta ggtcactcta ttatttatcc tagatctcat
     9121 tgtgtcaaat gctgtcgttc gctttcttgg tatgaattga ttccgattct ctcgatcatc
     9181 gtccaacgat ttcgctgccg ctattgcagg tgtcggttgc ccttctacta tttgttggca
     9241 gaagccttat gtggtggtct ttttgcctgg ttctttactt tttcttccgc aagaaatttt
     9301 tcgacgttca tatggatgct ttcggctttg ctattttcgt tgatggattt attttacttc
     9361 atggtccatt ctcacacgct attctgttct tgggcaattt tatggatttt ttggttgcag
     9421 gccggcgtct tccaatggca aagtgtgctg ttaacgttta tcgtaggcgg tgtgtgcctt
     9481 cgctatggtc aatcctatct cggagctggc gatactctgt tgatgctttc ttggagtggt
     9541 ggcttgtctc tagaagagtt gcttcagatc ttgtttcttg ctagtctgtt tggaatatcc
     9601 gttttttctg tttactttat ctgtcataaa aaaaagttgg aaaagctgcc tttcattcct
     9661 tttttgagca gtgcgttgct gattgtcctt catcttaggt attgaaaaaa ggttgtgaca
     9721 aacgacttgt cacaacctct gatatatagt gttctcttac caaatccttg tgacttggag
     9781 ctaggcgaca ggttcacaac ctctccacta aatggtgttc aatcagctga ccctcagtat
     9841 tagctcaaaa tgctggtttt acaacctctg atatacggtg ttccataagt agctttttcg
     9901 gcttcttatt tcttgacgcc ccaatgtcga cgaaggaaac ctgcacgtcc agaaccttct
     9961 cgataaagat tgtaatggag cttgtttttc ttgtaataat cctgatggta atcttctgca
    10021 ggataaaatt cgcttcttgg ttcgattttt gtcacgattg gcttatcgaa tcgcccgctg
    10081 tcttgcaatg cttgttttga acgctctgca atatttcgtt cttcttcatc attatagtag
    10141 atcactggac gataagaatc accgcgatca gcgaactgtc ctaatgcgtc agtcggatcg
    10201 gtttgctgcc aatagatttc caccaactga tcataagaaa taacttctgg atcatattcg
    10261 atctctactg cttctgtatg ccctgttgtg cctgttgtta cttgttcata agttggattt
    10321 ggtacatgcc cgccagtata accggatgtc acagaaatga tccctggctg ctcttcaaat
    10381 ggtttgacca tacaccaaaa gcatccgcct gcgaaaactg ctttttttgt catataaaat
    10441 cccccttttt tctcatcata ccttgatttc caaaaggagg ataacgtttt gcttgtgaca
    10501 gaaaaaacgg aaactattat atacgtgata cggcttctga taaatgccaa aacgaacgta
    10561 cttgatcaaa aacggttaaa tcaggaatca catattcagc agaaggaaga tttatgtttt
    10621 cacctattcc caatacatgc atgttaccag ccaaagctgc ttgacaacca gcttctgaat
    10681 cttcaatgac caagcatgca tttggcggta cattcaattg ctgtgctcct ttgagaaaaa
    10741 cttctggatc aggttttgct tttgatactt gcgtgccgtc aataaggaca tcaaaataac
    10801 ttgtcaaatt cgttttttct aaaatgaggc gtgcattttt gcttgctgat cccaatccga
    10861 tcttgatgtt tgtttttttc aagtactcaa gaacagctat tgcaccaggc aaaacggatg
    10921 tttcatccat tttttgaatc gcttgaagat atagattgtt tttttgctca gcaaaggctg
    10981 atttctcttt ttctgagaat ttctgttcca tttttccaaa tgacagcaat cgctctagtg
    11041 actcggttct ggatattcct ttcagttgct cgttttcttt ctcagtgaat ggaatggaca
    11101 gctggttagc taaaaccagc cacgcttcgt aatgataacg agctgtgtct acaagaacgc
    11161 catctaaatc gaatagtgct gctttgattt tcatcgatta cctccttgaa aatgaacgac
    11221 ttgaagtaaa gatgcgatgt ttttagctgt gcgatgaaga ttatcagtgg cttgctgtaa
    11281 agcttcttgt aaaggcacag ctgcaggaag gatcggaaaa acggccgaaa tgccaacatc
    11341 tgctgctttt tcgcttcctt taccaacact tccgcaaata gctatcacag gaacgccctc
    11401 tggtactctt ctagcaatcc caactggcgc ttttcctttt agactttgtc catctagacg
    11461 tccttcgcct acgattacga ggtctgcttg ctgtacccgt ttatcgaact ccagtaaatc
    11521 caatacgtag tcgatgccag agactatttt ccctgaagca aatgctaaca gtcctgcacc
    11581 cattccacca ccagcactac tgcccgatgc ctcgattact tgaggggcaa atctcatata
    11641 aaaatcatgc atggcttggt cagccactgc taattcattt tctttcaagc ctttttgtgg
    11701 tccgaaaaca aaagaagcgc cttcttttcc gcataaaaga ttagtaacat cagtaatgac
    11761 agtgattttg gctttaatca gctcaatagg cacgttcgtt ttatcaactg tagcaatttt
    11821 acctagattt tctcccagtg cttctacttt ttgattatct tgatcgtaaa aagcataacc
    11881 tagaccttct gccatgccga tgccgccatc gttggtacta cttccgccaa cgccgatgat
    11941 gatgtgtcga acaccttgtt gcaacaattg tcgtatcagt tctcctactc ctcgcgtgga
    12001 taacctaagt ggctgttgtt ggtttagtgg gacatctttc aatccgacga tttctgccat
    12061 ttcaaaaatc gcagtttgac cgtctgaagc gtactggatt tctttgtttt tgccaaatgc
    12121 attagtaacg actgctgttt gccgcttcaa ccttaaactg tctgctaatg atgaaaccgt
    12181 tccttctcct ccatcgccta taggtagcag atcatattca gcctgaacaa aaacttcttg
    12241 aaaacctgat tgaatggctt gtgctgcctc taaggctgtc agactttctt tgaaagaatc
    12301 tggcgcaatc agaatacgca ttattttccc ctccgttcca agatcagttc tttttccagc
    12361 tggtattggt ctccattgac aaaaataggt aatgtttctt tcgtctcttt ttttattact
    12421 atttgttttt ttgtcaaaac aaatagcaac ctgtgatttt tccaaataaa aggaaaagaa
    12481 attttttccc atgctgaagg aagtcttgga gaaatagaaa gagttccatt cgttactgta
    12541 atacctgaaa aaccaaatat aactgctagc catagtgctc ctaatgaagc agcatgaatt
    12601 ccgtcatctg atgaatgcgg atctggtcct aaatcaatca ggcacgcttc ttggaataaa
    12661 tgatacgccc attccatttc ttggatacgg caagcctcta tggcgtgaat cgctttactc
    12721 aacgatgagt cgtggattgt tttgttttca tagtaatgca aattcttaga aacgattggt
    12781 tgagaaaaat actcaggtaa taagtataac agcatgacaa gatctgcttg cttgctgact
    12841 tggagttcat ttacttcgct acgggcataa tcaagtaaaa tagactggct gccaggacgt
    12901 tctttgtaag tggtcaaatc aataactggc ttactgagat acgtgtcatc ttgcggaatc
    12961 agctgttctt tgttaggcac tggcaaatat aacttatcta aaaaattttg acattctttc
    13021 aaaaaaagcg gatcgccagc ttgatattta acagctaagg ccacattata atgcgccaga
    13081 tagttcgtat atgcattatt gtcgatatat tctgtgtatt catctggacc aatgactctg
    13141 tgtatctcta aatgatcatc gacttttaca gctctgctca gccaaaaagc tgctgtttca
    13201 tgaatgagcg tttttccctt tgtttctaaa aacgattgat cctctgttgc ttggacatac
    13261 tcttgaacgg cataagcaag gtctgcactg atatgatgtt ctgcggcagc tgaagcaact
    13321 tcttgtcttg ttccggtttt gatattcaat gcagcaaatt tgggtgtctc ttcctgacca
    13381 gtgtgtgcgc tttcccatgg gaaaagtgca ccttgatagt gataatctct tgctttttgc
    13441 cgtgcgcctt ctaatcgatc ataacggtac agcagcaaat tctttgctac ttcaggaaac
    13501 tgatataaaa agaaaggcag gataaaaatt tccgtatccc agaaaacatg ccctttataa
    13561 ccttcgccag ttagtccctt cgcccctaca ctaacacttg gatcattgcg gggagtcata
    13621 atctgtaaat ggtaagcagc gaagtccatc gctaactgat cgaaagaaga atcgccttgg
    13681 atcgttatcc gatgctgatg ccaaaaatct gtccaataag acatcgactg ctctaaaatt
    13741 tctgtataac taacgctttt ctgggcttgg gcaatacttt tcttaaacgg gtctgtttgc
    13801 tgaagagagg tagagagcac gatgatcttc tccagcgtaa aggacgtatt ttttctgatc
    13861 ttttctgaaa aggaagcggt gatccggcga ttttttgctt ggaatacacc tgctttattg
    13921 aatttagctg caatcccaat atcaatttta ctttctgttg tctggtagga tgcactgatc
    13981 aaatcgtcac caaatactcg cagttctttt tcaaccaact gctgtgtacc aaaattggtt
    14041 tgctgtccat cgatacccgt tttcacatct acttggcagt cttcggtcaa cggcttgatg
    14101 accactttca gggctgcctg atggagatta ttttgatcag caacacgaca agtaatgact
    14161 tccacttttt ttccttgagc agtttcaatg agcgcatagc gtttaagctc tcccatttct
    14221 aaatctagtg cccgctcgta tgcaagaact ttcatcgtct caagagaaaa atgttcaccg
    14281 tcgattttta tttcccatct gatgacatct gggagattca ctaactcggc tgattctgct
    14341 cctaaaggtc ggttatatac accagctgca aacattcccc ttacttgatt ccgatagtct
    14401 tcctcctggc ttcctctgat accaaaaaat ccatttccaa tggtcatctg actcgcatac
    14461 ttattgatat actctaaatc aaactgtttt tcttctactt tcataaggta ataacttctc
    14521 ctgtttctgc cgaacgatat aaattttcca ccagattctg gatgacatat ccttgccagc
    14581 catctgcaat catctgagtc tggtcaccga gcgcccgatt cacaaaatga gccatactta
    14641 gttcatgtgg gtcattaact ggcgtggtct tttgtaagga aaccagcttt ccatctacat
    14701 cttcgaatag ttcgcctgga tacagattag cacctgcctg atcgccacaa aaagccacat
    14761 tcagtttgtc ttctctgata ttcaaagcaa acgaagtatt cagttggatg atcttgccgt
    14821 tttctaattc cagtgtgcca aaaattgcat cttctactgt gaatttgctt ggatcccatt
    14881 tcccgaattt ccctgaattt tttttcgtac caattttttg gaagctgtac gcccgaacgc
    14941 tttttacttt aggaaatcca gtaatataca tcgctgtatc aagcatgtga atgccataat
    15001 cgatcaaagg cccgcctcct tgaagttctt tgtcgataaa cgttccccag cctggaacac
    15061 cagaccgacg aagcgcattc gcttctacgt aataaatatc cccgagaaca tcaagatgat
    15121 tcttgatcat tgttgcttcg tcagaaaaac gatgctggaa tccataagcc aaaatctgcc
    15181 cgtttttatc ggctgtctct gccatctttc ttgcttgatc agctgtcata gctggtggtt
    15241 tctcgcacat cactgaagca ttagcttcga gggcagccaa tacatgttcg aagtgaaagc
    15301 gattgggtgt acagacactc acgatatcgg gagaaatctc attcagcatt tgtgtaactg
    15361 atgtatacgc atgagaaaat cctgtacgtc ggcaaaagtc ttctgctcgc tggctgactg
    15421 gatcaaccac agcggccaat tctaagacat ctgatctttt ttgataataa gcagcatgta
    15481 ctttttctgc tacttgtcca gcgcctatga ttgctacttt cttcatttat ttctctctcc
    15541 tttgcttatt tacttattgt tattctttct ctattctttc tctaaatcat ttaaatgacg
    15601 cgctgtttcc agacagtttt tcaagtattg caatgattcc tgataaattt tcacttcatt
    15661 ttctcctcgc aaacgacatt catataccat ataaccttca tactcatttt gaagtagcgt
    15721 ttgaaaatgg cgagtaaaat caattgcgcc agatcctggt tggtagcgat gattatctgc
    15781 taaatggata tgacccacat actctttttg cgtagtcaat gcttttgaaa tatcatcttc
    15841 ttcaatattc atatgataaa aatcagcgat gatctgcaca tgtttcaatt gattagagtc
    15901 aatgtaatga cgcgcatccg ctaacaaatt gatcatatgg tcttggtatc ggttcaaagg
    15961 ctccaaataa ataaccgtac ctgtttcttc ggctacttta tctaaataac gaagtgattc
    16021 gctgactgct ttatagtctc cttctttgct tcggggtgaa accatcggcg gtaatcgata
    16081 agtgaacatt ccccaagctg ctgggataac gattcctttg cctccaacct cgcttagtgc
    16141 acgcaaaatc tcagcaattt gacgtacacc attaagacgc cgttcttcga taaagtcacc
    16201 gatccaccca tcgtagccgt tacaagctgt cactactggg atccctgttt ctttgattgc
    16261 ctgtttgact gtgtcgatat tttctattaa caacttaccg tcgatttcgt attcttcaaa
    16321 tccacagtct ttgataaatc taaatttttc taaaatgtct cttgggaaaa atgcttgatc
    16381 ttgcgtaccg aatttcattg ttctcctcct cggacttgaa cgcccatttt gatactttct
    16441 tctgggtgct ggtctacata acgcatgtaa gcttctgcgc tttctgcaaa tggcacaact
    16501 ggatcaatga tttcttcaca atccaagtaa ccgttcatca ataattccca acatgtgtcc
    16561 tcgattcgtt tacggttcca tcttgggtaa tctgggtttg gttcactaga tgctcgagca
    16621 aagacgattt ttgcattatt gaaatgtgct tcgcgaccaa gattaaaccc agctggaaaa
    16681 ggtttagcaa aggcaacata agaaatgatc ccgccatagc cgatccctat taaagaagct
    16741 tgtaatgcgg cagcactgcc actcgtttca ataactgaat ctactccacg tttatctgtc
    16801 aattccttta gacgaaaacc gacatctgct ttcataggat ctagcgtgta atcggcaccg
    16861 tgtttttctg ctaattctct acgctgcgct atgggatcga tcccaacgat ggtagtagca
    16921 cctgcttttt tagctaattg aatcgcaatt tgcccgatag cgcctaaacc gaagattgct
    16981 acagcatcac ctgctcgaac atgtgcttcc cggataccac ctaaagcgaa ttgagcagga
    17041 tcgtaacata ctgcattttt ccattgatcc aaccgatcaa gtttacgtag tttataatta
    17101 tcaacaccgt ttgtaatcag cgtttcacga atgggtccat aagtgcagac taactctcct
    17161 ttttgatact ctgtcaccaa actgcccgtt tcttcgatcg taccgacaat catatttcct
    17221 aattgaaatt cgccaaatac cacaccgcgt tggcttcctt cttctcttgg caagaatagg
    17281 cgccattctt ctgaaaattc ttcatcgata aatggactta atcctctaaa gtcgacaatt
    17341 tctgttcctg gttttggagt agcgaaaatt actcggatac gtacttcgtt cttttcgatc
    17401 tcacggtctt cgtaatcttt gaatccggct attcttggtt ctaatgcaac taattttttc
    17461 atcttttctt tcctctttct tttcttttta tccttttacg ccgccttcga ctaaatcacc
    17521 tttaataaat cgttcagata acgcatacat aatgatgact ggtaaagcgg tcaccaatga
    17581 agcggccatc attcgtcccc aaatgtagtc tggtgtactg aataatgtgt tcaaaccgat
    17641 tggtaaagta aatttttcgg aggtcgacag gaaaatcgaa gcaaataaat aatcattcca
    17701 cgaaaccata aagcaataaa cgaagacaga aatgattcca gaaatagcca atggtataat
    17761 gatccgaata atgatctgga tccggctaag accatcgatc attgctgctt cttcaatatc
    17821 agtcggaatc gtgtcaaaat aactttttag catgaatatc gcagtaggaa gtgtttgaac
    17881 gaccatcgtg ataatcagtg ctgtattggt atcatacaaa cctagaccgg aaatgatttt
    17941 aaataatgga acaacgatca aaataccgga aaacatataa acggtgtaaa agctagcatt
    18001 gattttcgag cgagctttga agcgtagttt tgacaaagcg tatgccccgc tgatcccaat
    18061 cactactgca gttacggagg ctatcaagga gatcactaaa ctatttttga aatacatgat
    18121 gaatggaaaa atctcagcat tgaaaatatc ctgataatgt tcaaaagtcc attctttcgg
    18181 aaaaatagtt tgtgtcgttg caattgcttc ttgtgaatcc ttgaatgatg tcatcagcat
    18241 gatcaaaaat ggaaacaaat tgacagctaa tagcaacaaa atcgtcgcgt aaaataagat
    18301 tttcctgctc tttttatttt tcatttcaag tccaccttct ttcgtgagag caagacgacc
    18361 gctgcaataa tgatgaacaa tagaatcgat atagctgctg cttttccaag atcattgaac
    18421 gcaaaggctg tctgatacaa ataaacgcct aagatattga ctttattcgt cagtagatag
    18481 acatctgtaa acatatagaa catccagata gatcgtaaaa caatcacagt tgctagtact
    18541 ggtttgattg ccggcagtgt cacgatcttg aacttctgcc aagcatttgc accgtccatt
    18601 tcagctgctt cataaagcgt tttgtcgact gtttgcaaaa tagccaaaaa tgaaataaac
    18661 gcatacggat agtatctcca gatagcaaac aaaacgacca taaagaagct agcaactggc
    18721 tggtcgaacc acagcggtgc ttcgttgaaa agctgcaacc tatctactaa cagatcattg
    18781 acgattccat aactattatt gaacatatat ttccaagcaa aaatcaaaga aatagatggc
    18841 gtaacataac ttaataaaat cagtgaacgc accgttttcc taaatctgaa cttgcggtta
    18901 agcaaaatag ctacggctaa tcctaaagct gtactgcctg ctacaactaa gaatgtgtaa
    18961 gcaactgtta aacctaaaga acgataaaat tcactatcac ttaaaatttc aagataattg
    19021 cttaatccga taaaaacggc gtctgagtta ggattcagcg gcatatcgaa aaaactgatc
    19081 tgaatattgg aaagcattgg aatcagcact aaaacagcta ataaaagcat gcttggtgct
    19141 aaaagtccca tggctaattt taagtcagaa tgtttccttg gacgattcat tgaaacctcc
    19201 ccctgataat ctgccaatag ctatctactt ttttcgaaca ccattggcag aacttcttgt
    19261 tatttcgtat ttttttgtgc gtcagctaaa gcttgatcga catcttcgtt tccgacagtt
    19321 acttggttca ctgcttttcc gatcacacct gagcttgtga tatctcccat tttcgtgaaa
    19381 ttcttttccc cgactagtcc aaagacttgt acttcatcaa aagcactagc aatctcagat
    19441 ggcagctcgc caaaagactt gatgacttca ttttctttgt aaccatccag ttcgactact
    19501 tgtttattca ctggttgagc accaccagga gacatcaaga cccacttttc catgtttttc
    19561 ggttgagata agaaagctgt gaatgcttca gcagcctctt tttgtttctc gtctagacca
    19621 gaagaaatcg tcaatccaga tactgtgcca tagacagctt gatctttttg tgttgggata
    19681 gcaaaaccga tattttctga gttccccgct tcataaacag aaggcaaaat gtaagttgaa
    19741 tagattgcca ttggtgctgt tccattcatg aaagcatctt tgatttctgt cacatcatta
    19801 gagcctggca tcgtgtattg ggataagttt tgataaaacg acaatgcttc tctcatcttt
    19861 tccgtatcga tcgttacctc gccttcatca tccaaaacat ttgctccatt tgacaaagca
    19921 aattgagaga acgcttgctc actcattgtt ccttcagcag taggcatagc aattccatat
    19981 tgtttattct ctttatctgt gaaatgttga gcaattttta aaatatcatc ccaattttct
    20041 ggttctgaaa aaccagcttc tgataatttt tctttgtcat accaaatacc ttgaacccag
    20101 ccgctaattg gtgcagcaat atagcttttt ccatcttctg aacgaacaag gtttttggct
    20161 ccgtcataat aattatcttc tccagcttcc tcgataacgt tttggactgc cgtctgatca
    20221 atcagttcat ctttatccat cactttagca aaatcctgac tgacttccat tacttctggt
    20281 aatttaccgg aacgagctaa cgtaacgatt tttgtattga acgctgattc ttctaccggc
    20341 acttgcttga ttttgatgtc tggattttct ttttcaaaat cagcaaccag ttcattgatc
    20401 acacttaatc gttcctgttc gacagaagag tgcatgaatt gaatcgttgt ctgttgtgat
    20461 tcgttagagc tttgactttc accacaagca gtaagaagaa atccagtagc tagcagacag
    20521 gtagtaacaa taattttttt catttttttc cacctcttat tttattattt tcagccacat
    20581 atattcatac ggctggaata tcaactttcg ttcttgactg caatggataa tttgtccaga
    20641 aaacaggtct aggtaatctc cttttggtaa aagataacct gctgattgat tggaaaaatt
    20701 atggataact aagactttct cttccttcgc ttcgcgaata aaactgataa aagaatctgc
    20761 tgagtccagt aagtaaatca aactattcgg atggaaggct ttttcttttt ttcgaataga
    20821 tagcatctgg gtcaattcac catatactgc tgttcttaat ggatctgtct gcaactcttt
    20881 ctcaatatct gctaacaaaa atttttgccg attgattttt cgattctctc ctgttttttc
    20941 tacgcctttg taatcatttc gacttcccag cacactttga atataaattg ccggcacgcc
    21001 tggtaacgtt accaaaaatg aatgagcagc taaaaagcgt ttgcttctcc attccgtcgt
    21061 atctgttgga gaacttaacg catctagata cgtagcgttt atctcatatg gactggtcgt
    21121 tccatctggg tttttcttgt agtttactcg ggcaccctct ttcttcagct gttccactaa
    21181 ttcaaggatc gttttttccg gcagaatacc tcgtaccgga ttcaatccga taccgtcatg
    21241 ggaggctaaa aaattaaaaa aagttgtttt tttcgatgcg aaatcgatgg tttttaacca
    21301 ttctttcaac ttacttgtat tacccgaacg aatcgcttga agggtcaaag gtggtagcga
    21361 aaactgatag accatctgtg cttcattctc gccattccca aaataactga tattatcttt
    21421 gtgtggcaca tttgtctcag tgatcaatac cgttcctgga gctgcttcat cagcgattgc
    21481 acggaataat tggatgattt tatgcgtttc tggcaggtgt atacaagaag ttcctagttc
    21541 tttccataaa aatcccacag cgtccaagcg aataaatgaa gcgccttttt ctaagtaaaa
    21601 caaaagaata tcgatcattt tcaataaaac ttctggattt ttaaaattca agtctacttg
    21661 atcttcgcta aaagtcgtcc atacgtgttt ttctttcccg tttttgtcaa tgaacgttgt
    21721 aagtaacggg ctggtccttg gtctagtcac gctgcttgta tctgtatcca gtggcaaatc
    21781 aataaaaaaa tctttgtacg tatcattcaa gtttaaatat tcctgaaacc aagtactttc
    21841 ggcagacata tgattgcaga caaagtcgaa cattaaccgt gtttcgcttc tcatttcttc
    21901 tatatcttcc catgtgccca aacggtcgtt gattgcctga taatttacaa cggagaaacc
    21961 atcatcagat gtatatggga aaaatggcaa gaaatgtaca actctaaatg tatctgccaa
    22021 atacttttga taaaatttct taaatgtacg caaagttggt tgctgctctt caaaaaattg
    22081 atccccataa gtaatcaaga aaatatcttc ttcgtcccaa tgcagtttgc ctgcctggcc
    22141 tttcttctgc tgataaacac tgactttttg ttcaatcata gaggcggttt tctcagacaa
    22201 atctgagtcg cgataaatcg cttgtaaaag ttttgtatac atcggattcc cctttctttt
    22261 ttgggagcgt tcccaacgat ttgatcatat tatggaaaac gcttactgtc aatagcattt
    22321 ttttaaataa ttttgatata cacaaaaata caaataagaa agcttatgta tcaataatta
    22381 atagcttctt aaaaaaatat ggtctctcat gatcatgcaa gctattccta aattttcaaa
    22441 atcatagaca aaaaagaatg tctattgtac tatggaatta ttctaaaacg ctcccaaaaa
    22501 ggaggtgcat ataaaattga aacctactat ttatgacatt gcgaaaaaag ctaatgtctc
    22561 aaaatcaact gtctccagag tattaaataa ccagccaaat atctctgata aagcaaaagc
    22621 aagtgtactt caagcaatca aagaattgaa ttatgaaccg agcaaaattg caagaggact
    22681 gtcaggaaat ggttttgacg cgattctagc attatctcat cgtacttctc atacgaccta
    22741 tggcaatcct tttttttcag atgttattca atccatttct gccgtttctg aacaaaataa
    22801 cttcgacttg attctacaaa cggcaaaaac accagaagaa gagctagaaa aatgcgcgaa
    22861 aaagatccaa gaaaaaatga tccgaggaat tattatttta agctctacga tggatgaaac
    22921 acttcttttt gaattggatc aatacaatat tcctattgtg gtaatcggaa aagtcggaaa
    22981 cgactacacg aacatctact cagtggacac ggataatttc caggatagtt acgatttggt
    23041 ggaaacactg atcaagaatg gacaccgtca tatcggctgt ctccatgctc cgacagaagt
    23101 ttatgtatcc aatgatcgtc tcaatggtta ccgtcaatgc ttatttgatc accacctaga
    23161 tatccgtaat gaatggatta tagacggcgg ctttcgctta gaagatagca tggcagcaat
    23221 gaatgattta atgaagctac gagaaagacc aactgcgatt ttttctaccg atgcattaaa
    23281 atcattgagt atttacaaag tggcagaaca atctggaaag cagatacctg atgatctttc
    23341 gatcgcagga ttcagtgatc ccttattgtc ctccctattt tcgccacagt tgactcggat
    23401 agacattccg acaaatcagt taggaatcaa agccacagaa cttttattcc aactaatcca
    23461 ccaccaatac cctgaagaaa aaagaatcat tctttcaacc gagattactc tcacagattc
    23521 gatccaacaa caagaaatgt agaacagaat tataaaagaa cagaaacact gaggataagt
    23581 cagttactct gttctttttt caatcactca tccctcgtta ccccctcccg gtattttagc
    23641 gaatttttct tattttccat ctgttttaga tagctgtgga taactataat gtttgtgtta
    23701 aactattttt tccacaggga agaaaaaact atttttaacc acgttgtgta taagttttta
    23761 tagttcgttt ttttaaacac atttaccctt atttcaatcc atgttatttg gggatactta
    23821 tctctaaaac ataaatagaa taacagacat gtctactggt tttgttttat tcgaggaatt
    23881 attcaataat agctagcaaa atattaaaaa taaccgctct atcttcccat caatttattt
    23941 tgcattcaaa aaatgatcta tctttacttt tgttgattga cttctcttgg aagatgatcg
    24001 ttccgatacc ttaattggat acgaaccgat gttgttaaga tcctttccca cttttcttct
    24061 agttagacaa attattttag acaaaccaaa aaagaggttg tgacaataat ttgtcacaac
    24121 ctcttttt
//
LOCUS       DAGCYL010000021        23943 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.021, whole genome shotgun sequence.
ACCESSION   DAGCYL010000021 DAGCYL010000000
VERSION     DAGCYL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.021"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            39..791
                     /locus_tag="MB216_000684"
     CDS             39..791
                     /locus_tag="MB216_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4525542.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            788..1702
                     /gene="pfkB"
                     /locus_tag="MB216_000685"
     CDS             788..1702
                     /gene="pfkB"
                     /locus_tag="MB216_000685"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBT4525543.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1720..3651
                     /locus_tag="MB216_000686"
     CDS             1720..3651
                     /locus_tag="MB216_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4525544.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3762..5321)
                     /locus_tag="MB216_000687"
     CDS             complement(3762..5321)
                     /locus_tag="MB216_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBT4525545.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5476..7710
                     /gene="pcrA"
                     /locus_tag="MB216_000688"
     CDS             5476..7710
                     /gene="pcrA"
                     /locus_tag="MB216_000688"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBT4525546.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7727..9763
                     /gene="ligA"
                     /locus_tag="MB216_000689"
     CDS             7727..9763
                     /gene="ligA"
                     /locus_tag="MB216_000689"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBT4525547.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9869..10174
                     /gene="gatC"
                     /locus_tag="MB216_000690"
     CDS             9869..10174
                     /gene="gatC"
                     /locus_tag="MB216_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBT4525548.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10174..11646
                     /gene="gatA"
                     /locus_tag="MB216_000691"
     CDS             10174..11646
                     /gene="gatA"
                     /locus_tag="MB216_000691"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBT4525549.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11643..13073
                     /gene="gatB"
                     /locus_tag="MB216_000692"
     CDS             11643..13073
                     /gene="gatB"
                     /locus_tag="MB216_000692"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBT4525550.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13083..14138
                     /locus_tag="MB216_000693"
     CDS             13083..14138
                     /locus_tag="MB216_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBT4525551.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14249..15622
                     /gene="rlmD"
                     /locus_tag="MB216_000694"
     CDS             14249..15622
                     /gene="rlmD"
                     /locus_tag="MB216_000694"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBT4525552.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15625..15753
                     /locus_tag="MB216_000695"
     CDS             15625..15753
                     /locus_tag="MB216_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBT4525553.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            16177..16365
                     /locus_tag="MB216_000696"
     CDS             16177..16365
                     /locus_tag="MB216_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBT4525554.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            16443..17456
                     /locus_tag="MB216_000697"
     CDS             16443..17456
                     /locus_tag="MB216_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4525555.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(17523..18557)
                     /locus_tag="MB216_000698"
     CDS             complement(17523..18557)
                     /locus_tag="MB216_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4525556.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            18672..20897
                     /locus_tag="MB216_000699"
     CDS             18672..20897
                     /locus_tag="MB216_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBT4525557.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            20915..22798
                     /locus_tag="MB216_000700"
     CDS             20915..22798
                     /locus_tag="MB216_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBT4525558.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            22779..23753
                     /locus_tag="MB216_000701"
     CDS             22779..23753
                     /locus_tag="MB216_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBT4525559.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         8017 a         4376 c         5180 g         6370 t
ORIGIN      
        1 aaaaatatgc tattatttgt attgaggtga tcgtgaaaat gcttacagag gaacgtagac
       61 aaaaaatcct agacttgctt gaacagcaga aaatcgtgaa atcacaagat cttgtaaacc
      121 tttttaatgc ttctgaatca acgataagac gtgatttaca agagctggag aatagtgggt
      181 ttctagaaag agttcatggc ggagctaaga aagagcaatt attgaatttt gagcagaata
      241 tgagtgaaaa atcactcaaa aacgttcacg aaaaacagca aatcgccaaa tcggcagctg
      301 aaaccattaa tgacgaagat gtcatttatt tagacgctgg ctctactacg ttagaaatga
      361 ttccttttct gcaaggaaaa cagatcacag tagtgacaaa ttccgttcaa catgcggcaa
      421 atttagttga tttggagctt gctaccatta ttttaggtgg aacgataaag ctggcaacta
      481 atgctgtttt aggatctaat gcgatagaac aactaaaaaa ttttcatttt aataaagcat
      541 ttatgggaat gaacggtgcc catcttgaac aaggattcac gactccggac ccagaagagg
      601 cagctgttaa acgtttagcc atgaaaaatt ccgaagagac atttgtattg attgatcata
      661 ccaaattcaa ccagttgtcc ttcacctctg ttgcaccttt ggaagcagcc acgatcataa
      721 ccgagcgttg tccattagaa ttcgttaatg aattccgtga aaagacaaca attaaggagg
      781 caatacaatg atttacacag taacacttaa tccatcaatt gattatatcg tacgcgtaga
      841 tgggttaaaa ctaggcgatt tgaaccgaat gaaggaagac ttcaagttac ctggaggaaa
      901 agggatcaat gtttcccgta ttttgaaacg gatcgattct caatctacag cactagggtt
      961 tttaggaggg ttcactggcg aatttatttc gaagtggctt gaaaaagaac aaatccagtc
     1021 tttgttcaca accgtaaaag aagatacaag aatcaatatc aaactaaaat cagatactga
     1081 aactgaaatt aacggtttgg gcccatctat ttcagatgaa gaaatcgatg agttgaagca
     1141 agcattagga acagtaggac aaggagatat cgtcgtattg tcaggaagcg cgccggctag
     1201 cctgcgaaaa ggattttatc aagaactgat tgatatcatt cgagaaaaag gtgcagattt
     1261 cgtgattgat acaacaggtg atgacctaaa aaatgcactt gttaagaaac cattgttgat
     1321 caaaccaaac aatcacgaat tagctgagct ttacaataca accttccaat ctgtagaaga
     1381 aatcattccg tatggtaaaa aattgctagc tgaaggggca cagaatgtgc tgatctcaat
     1441 ggctggagac ggtgctttgc tcttcacgga acaaggaact tataaaacga atgtattagt
     1501 tcgcccattg aaaaattcag taggtgcagg ggattcaatg attgccggct ttattggggc
     1561 ttacagccgt acgaaagatc caatcgaagc ctttaaatgg ggagttgctt gcggaagcgc
     1621 aacagctttt tctgatgact tggcagctcg cgaattgatt gatgagttgc tgccagaagt
     1681 tgaaatcaca aaaattgatt agtttcaagg aggataatca tgaatatcaa ggatttaatg
     1741 gtcaaagaag cgatgatcat ggatttgcaa gcgactgata aaaaaggcgc aatcgatgaa
     1801 atggtccaaa aaatgtttga tgccggccgg atttccgata tcgaaacata taaagaagga
     1861 attctagccc gcgaagcaca aacttctaca ggtcttggtg atggaatcgc catgcctcac
     1921 gcgaaaaaca gtgcagttaa agaagcaaca gttttattcg caaaaagcca aaaaggagta
     1981 gattatgaag ccttagacgg acagccaact tatctgtttt tcatgatcgc agctccagaa
     2041 ggcgcaaacg acacacattt gcaagcatta gcagcactat ctcgattgct gattgatgca
     2101 gatttcgtag gaaaattaaa agaagcaaat acaccagaag aagtccaaga cttgttccaa
     2161 acagctgaag agcaaaaaga agcagaagaa aaagcagaac aagaaacaca agctgcagaa
     2221 gcttctgctc atccagaaag aaaattcgtt gttgccgtta cagcttgtcc aacagggatc
     2281 gctcacactt acatggcaga agatgctctg aagaaaaaag cgaaagaaat gggtgtggag
     2341 atcaaagtcg aaacgaacgg ttcagaagga atcaaaaacc gattgactgc tgaagacatc
     2401 gcccgtgcag aaggtgtaat cgttgcagca gacaaaaaag tagagatgaa tcgctttgac
     2461 ggaaaagaac tgatcaatcg tcctgtcagt gatggtatcc gtaaaccaga agagctgatc
     2521 aatctagccc ttagtggtac tgcaccagta ttccatggtg atggaaaaga aagcagttca
     2581 gaagaaggaa atgcggatgg tactatcgga caaagaattt acaaagattt gatgaacggc
     2641 gtatcacaca tgttaccttt cgttattggt ggaggaatcg ctattgcgct ttctttcatg
     2701 atcgaccaat ttattggcgt gccacatgat caattatcaa atctagggaa ttacaatcaa
     2761 gctgctagct ggttcaacca aatcggacaa gctgcatttg gtttcatgct gccggtatta
     2821 gcaggattta ttgcttcaag tatcggtgac cgccctggtt tgatcgttgg atttgctgct
     2881 ggtgcattag ctaatacagg cggtgcagga ttcttaggtg cactgattgg tggattctta
     2941 gctggttatg tcatcgtatt tttacgtaaa ttattcaaga acttaccaaa atcattggat
     3001 ggaattaaaa cgatcctctt ttatccagtg tttggcttac tgattactgg tttcttgatg
     3061 ttgctggtca atgtccctat gaaagcaatc aatgatgcat tgaacggttt cttgacaagt
     3121 atgagtggtt cgaatgctgc tttacttggt gcattattag ctggaatgat ggcggcagat
     3181 ttaggcggtc caatcaacaa agccgcttat gtgtttggta cagcaacttt agctacgact
     3241 gttgcaactg gcggaagtgt cgtgatggct tctgttatgg ctggaggaat ggtacctcca
     3301 ttggcaattt tcgttgctac acgtttgttt aagaataaat tcagcaaaac agatcaagat
     3361 gctggtttga cgaacatcgt gatgggacta tcgtttgtca cggaaggatc gattccattt
     3421 gcagcagctg acccgattcg tgccattcca agttttatta ttggttctgc tttaactggc
     3481 ggattagtcg gagctttcgg gatcaaatta ctggctcctc atggcgggat tttcgttgta
     3541 ttcttactga gtcacccggt catgtatttg gtgtttattg ctatcggagc agtcgtatct
     3601 ggtgtgattt atggtttatt gagaaaagct cctgcagcag cagaagcata gaaataaaaa
     3661 agaagaggct ggtttttccc agtctcttct ttttttatag aatcatatag aatcaagtag
     3721 tgtagatgaa tagttgttgt aaaatagttt ttccatgttc gttaaaatga ttgcttcaaa
     3781 tttgtgatag aagatttagc ctcgatttcc tgcttgattc ttggcaagtt tgcttcatca
     3841 gtcaagataa ccaaaatatc tcctacattc atgatcgtat caccgtgagt caagatttcc
     3901 tgatttcctc tgcgtataga gattagcagc atatttttag gccaattgaa gtctcgtacc
     3961 attaatccgt ctaaagaact ctctgccgtt actggaaaat caaaagttgt ccttgcacct
     4021 tttagtgagg cggtctgagt atttccaagt agacgttcca ataatgattc gtaaattgga
     4081 ttaccgccga gaaggtcatt gacgatataa gcaacaagag cagccaatcc taaaggcatc
     4141 agatgattaa gagagccaac catttctgtc actaaaataa ttgctgtcag cggtgctttt
     4201 ccaatcgctg tgaaataacc agccatggca ataacgacaa aatccttgat gtagattgga
     4261 tccatcccga aatcaaccat aatgtttcca taaaagctac cgattaaagc ccccaatgtc
     4321 aatatcggaa ggaaaatccc ccctggaaga ccaccaccgt aagaaatcat ggaaaaaacg
     4381 aatcgaagaa caaacaaacc gataatcata atgattggca aggaagcatg tccaaaagct
     4441 aaaacgattt gattgcctcc gcctagagta ctaggccata aataaccaat tggcaaaatc
     4501 agcaagaaag gaacgaaccc gtgaaaataa gctggtaaag gcaatcggtt gtaaaatttt
     4561 ggcaaagcca gcaagacctt ctgataaata aaacctaaga tgcctagaag tactcctaag
     4621 atcactaagg aaccataata tttcaacggc aaagaaggaa ggtcacctag atataaaaca
     4681 ggtctcaagc cgaaaaagta aagagagacg aagttagcag tgattgctga agcaaatgat
     4741 gtcagccaaa caagtggaga aaaggagtga tggatttctt ctaaaacaaa gagtaatcct
     4801 gcgataggag cattgaatgc tgctgcaagt ccgctacttg caccgctaga aattaaaatc
     4861 ttttcttctg aagatggact ccgaaacaat tgtccagctc cttgtccaat ggatgcgcca
     4921 agctgtatag aaggtccttc tctacctaga aaaaggcccg caccaactga aagtacaccg
     4981 ccaatgaact ttttccataa aacagagaac cagttcattg ataattcccc gcgaagctgt
     5041 ccctctactt gtggaatccc gcttcccttg atatccggtt catttttgac cagataaccc
     5101 acgaaaaatg ctgcaaggat gcagcaaata gctaggccaa ttaagtaaat cggctgttcc
     5161 cgactcattt catagatact tcgaactaaa tcgcttagct tttcgataga taatcgaaac
     5221 aggctaacga cgattcctgt cagcagtcca acaaatattc cttttagaat aaaatatatc
     5281 tgagtcttat ctaatttctt gatttttcct tttttttcca ttcaaacgcc tcgctttctt
     5341 ttaatttagc gcgaaatcaa cgaattgaca aggaaaaatc cataaaaaga aacaaatgat
     5401 tttgaactag ctagtttaat gaaagccaag aaaatgctat aattaacaag attgataaaa
     5461 gaaaggatgt tgttaatgtc atccaaagct gaattattaa acgggatgaa cccccgtcag
     5521 aaagaagctg ttcttcacac tgacggacca ttactgctaa tggcaggtgc aggaagtgga
     5581 aagacgagag tgctgacaca ccgcattgcc tatttgattg aggaaaaaga agtcaatcct
     5641 tggaacatcc tagcgattac ttttaccaat aaggctgcaa aagaaatgaa agaacgtgtc
     5701 aatgcgattc tcgcatctgg aggcgaagat gtctgggtat cgacttttca ctcgatgtgt
     5761 gtgcgtattt taagaagaga tgttgatttt atcgggtata accggaactt tacaatcatc
     5821 gattcttctg aacagctgac cttgatgaaa cggatactaa aagaactgaa cattgatccg
     5881 aaaaaatatg atcctcgaag tatattagga acaatctctc aagcaaaaaa cagcctgcag
     5941 acccctcagg attttgcaaa aatgcaaggc agttattacg aagagattgc ggcaaaatgt
     6001 tatgctgctt accaaaaaga gcttcaatat aatcaatgta tggactttga cgacctgatc
     6061 atgaatacga ttcgattatt tgaagaacat cctgattcac tcacatatta tcaaaacaaa
     6121 ttccattata ttcatgtaga tgagtaccaa gatacaaatc acgcacagta tacgctagtc
     6181 aatttattgg caggtagatt taggaacctg tgcgtcgtgg gagatgcaga tcaaagtatt
     6241 tacggctggc gcggtgcaga tatgcagaac atcttggatt ttgaaaaaga ttatccagat
     6301 gctgcagtaa ttttgctaga acaaaactat cgctccacga aaaacatcct tagcgcagca
     6361 aaccaagtca ttgaaaacaa tagcaatcga aaaccaaaaa acttgtggac agaaaacaaa
     6421 gaaggaaaca agatcactta ctaccgtgca gataatgaac gagacgaaac ccgttttatc
     6481 gtagatcgga tgcaagaaga gattcgaagc aatcatcgga attacggcga ctttgccatc
     6541 ttgtaccgga cgaacgcaca atctcgtgta atggaagaaa ctctgttgaa agcaaatatt
     6601 ccttataaaa tggttggagg gcacaagttc tacgatcgta aagaaatcaa agatatttta
     6661 gcgtatttga atgtgctggc aaatcctcaa gattcaatca gttttgagcg tatcgttaat
     6721 tcgcccaaac gaggcatcgg accaggatct atcgaaaaac tacgcagttt tgcttctctt
     6781 catgaatggc cactgcttga agcagcgcaa aatgtggatc tggcaaatat cggtggaaaa
     6841 gcaggacagc aattaggtgc atttggagag atgatccaag aagtcactca gatgatccca
     6901 tacttgacag tgacagaatt gacgaaagag gttttggatc gtagcggata tctagaagac
     6961 ttaaaaatcc aaaatacatt agaagcacaa gcacgcatcg aaaacttgga ggaattttta
     7021 acggtcacac aagaattcga caaacaattc gaacagcaaa atgaagaaga cgctgatgca
     7081 ccagaagaaa aactaacggt cttcttaaat gatctggcac ttgtttccga tattgacaat
     7141 ttagaagaag atgcttccca agtgacattg atgactttgc atgcagccaa aggattagaa
     7201 ttcccagttg tattcctgat tggtctagaa gagggggttt ttcctttatc tcgagcattg
     7261 atggaagaaa gcgaactgga agaagaacgc cgtctcgcat atgtggggat cacgcgagca
     7321 gaagaagcgt tgtatttaac aaatgctttc tccagaacat tatatggacg gacgcaatat
     7381 aaccgacctt ctcgttttgt agaagaaatc gatcaggagt tactggaaat cgaaggcatg
     7441 cgtcctacgc ctaaaaaaac acctgtattc gctaaaaaaa cagcatatag ctataaacag
     7501 ccagagacag cggttgttcc ctctaaaagt gctactggag gggaaaataa cagctggaaa
     7561 cctggagata aagtcaaaca caaaaaatgg ggtctaggaa cagtcgtccg agtaagcggg
     7621 acgtcgaagg atctagaact agatgtggct ttcccaagtc aaggagtaaa acgtcttttg
     7681 gctgcctttg cgccaattga aaaagcttag tagtggaggg taaatgatgg aacatgaaat
     7741 gacgattgac gaagctgcta aacgagcaga agaacttcgt acaagactga accaatggtc
     7801 tcgcgaatac tacgtagaag ataaaccaac agtggaagac tatgtttatg ataaagaata
     7861 cgcagaatta gtagcaattg aggaacaata tcctgatttg atcacgtctg attctcccac
     7921 tcaacgagta ggcggaaaag tattagaagg ttttgaaaaa gtcactcatg atattccgct
     7981 ttacagtttg aacgacgttt tcagcaaaga agagctgata gcttttgatc aacgtgtaca
     8041 aaaagcagtg ggacgtgtag ttgactattg ctgtgaacta aaaatagatg gactttccgt
     8101 ttctttgcgt tatgaagatg gaaatttcgt gagaggtgcg actcgcgggg atggaactgt
     8161 aggagaaaac atcactgaaa acctaaaaac tgtccggtcg gtcccaatca aattaaaaga
     8221 accaatgaac atcgaagtaa gaggcgaatg tttcatgcca aaacggtcat ttgtccaatt
     8281 gaaccaagac agagaagcag agggaaaaga tatctttgcg aatcctcgga acgccgcagc
     8341 tggttctttg cgtcagttag attctaaaat cacagcgaaa agaaatctgg acactttttt
     8401 atacacagta gctgatttcg gtcctatgca agcaaaaacc cagtatgatg cgctagaaga
     8461 gctagaaaaa attggattcc acacgaatcg ggaaaaacgt ctatgtcatt cgattgatga
     8521 agtgtggtca tacattgaag aatatcatga caaacgtgtg gatttgccct atgaaatcga
     8581 tggaatcgtc atcaaagtga atgaattctc gctacaagat caacttggtt ttactgtcaa
     8641 agcaccgaga tgggcagcag cttacaagtt tccgccagaa gaagttgaaa cgctaatcga
     8701 aaacatcgaa tggacagtcg gtcgaacagg ggtagtaacg ccaacagcta tcatgacacc
     8761 agtccgagta gcaggaacga cagtaagccg tgccagcctg cataacggtg attatattaa
     8821 gttgaaggat atccgcttga aagatacagt tctgatttat aaagcgggag acatcatccc
     8881 agaagtttcg caagtcgtgt tagataaacg acctaaagac agcgaagaat accaattgcc
     8941 tacacattgt ccagtttgtg gaagtgaact tgtccatttg gatgaagaag tagccttacg
     9001 gtgcatcaat cctaaatgcc ctgctcaaat gaaagaaggt ttgaaccact ttgtttcaag
     9061 aaatgcgatg aatatcgatg ggttggggcc gcgcgtacta gaacagatgt atgataaaaa
     9121 actagtagca gatgttgctg atctttacaa attgacagaa gaagagttat tgacgttgga
     9181 caaaataaaa gaaaaatcag caaataatat tttgacagca atcgacaaca gcaaagataa
     9241 ttcggtcgag cggttgattt tcgggttagg aatccgacat gtcggagcga aagcagcaaa
     9301 aattttagca gaacatttcg gtgatctaga aacattgagt aaaagtgact atgagtcgat
     9361 tattgcgctt gatacgatcg gtgatatcat tgccgacagt gtagtcactt atttcagtaa
     9421 cgaagaagtc catgaattga tgaatgaatt aaaacaagca ggcgtcaatt tcgaatacaa
     9481 agggcttcga aatgcccaat tgcaagaagt cgagtctcct tttaaagaaa aaacagtagt
     9541 ccttactggt aaactaacac gatttactcg tgaagaagca aaagagacga tcgaaaatct
     9601 tggcgggaaa gtgactggta gcgtatcgaa aaaaacggat atcgtggtag caggagaaga
     9661 tgctggcagc aagctgacca aagcacaaga attaggaatc gaagtttgga cagaagatca
     9721 aatggcagat gcattggcta aaagtcgctc agtagaagaa tgaaaaatga gttttttcag
     9781 acaaatttca ataaacaatt tcagacacgg cactcttgtg ttaaaataga gagcaaagtt
     9841 tttaactcgt ttagaaagaa ggggtagtat ggcgattagt gaagaacaag tgaaacacgt
     9901 tgccaaatta gcaaaacttg cgttcgcaga tgaagaactt gcaagtttta ccgaccaatt
     9961 aggtaaaatc atcgatatgg ttgaaatgct ggaagaagta gatacagaag gtgttccatt
    10021 tacgtcgaat gtggttgaaa cagtaaacgt gatgcgacaa gatcgggcgc aagctggatg
    10081 gaaccgcgat gaattattga aaaatgtgcc ggaaacagaa gacggcttta tcaaagtacc
    10141 cgcaatcatc gataatgggg aggctggcgc ataatgacag aattacacaa tcgttctcta
    10201 gaagaactgc atcatatgct tgtatcgaaa gaaatcactg tacaagattt gacaaaagca
    10261 acatttgaac gaatcaaaga aacagagcct gaggtcgaag cattcattac attgaacgaa
    10321 gaaaaagcat tggaacaggc aaaagcttta gatgaaaaag ggattgatga aagcaacgtc
    10381 cttgctggta ttccaattgg tatcaaggat aatatcgtaa caaaagatat cttgacaacc
    10441 gctgcatcta agatccttta caattttgaa ccaatttatg atgcaacggt catggataaa
    10501 atctatcaag cagatatgat ccctgttggt aagctaaaca tggatgaatt tgctatgggt
    10561 ggaagtacag aaacttctta tttcaagaaa acaaaaaatg cttgggaccc aacaaaagta
    10621 cctggtggct cttctggtgg ttctgcagca gcagttgcag ctggacaaat tccagtttct
    10681 ttaggtagtg atactggggg aagtatccgc caaccagcag cttttaacgg catcgtaggg
    10741 atgaaaccta cttatggccg tgtctcacgt tttggtttga ttgcctttgc ttcatctctt
    10801 gaccaaatcg gaccaatgac ccgtacggtg aaagataatg cattagcatt gacagctatc
    10861 agcggttatg acgaaaaaga cggtacttct tcaggcgttt cagttcctga ttttgcagag
    10921 ggactgacag gcgacatcaa aggaatgaag attgctttac ctaaagaata cttaggcgaa
    10981 ggtgtggccc ctggcgtaaa agaagctgta ttgaaagcag cagaaacatt ccgttcatta
    11041 ggtgctgttg tggaagaagt cagcttgcct cattcaaaat acggtgtggc tgtttattat
    11101 atcattgcct catcagaagc aagttccaac ttgcaacgat tcgatggtat ccgctacggt
    11161 tatcgctcag aaaatgtgaa atcattagat gatgtatatg tcaactcacg ttcagaagga
    11221 ttcggtgaag aagtaaaacg ccgtatcatg ttaggtacat tctccttaag tgctggttat
    11281 tatgacgctt acttcaaaaa agcgggacaa gtccgtacat tgatcaaacg agattttgaa
    11341 aaagtctttg cagattatga cttgatcatc gggccatctt caccaacagt tgcattcgga
    11401 atcggtgaga acattaacga tccaatcaca atgtacatga gtgacatctt aacgatccct
    11461 gtcaacttgg ctggacttcc aggtatgtct gtaccggcag gtttatcaga aggattacca
    11521 gttggattgc agatcatcgg taattatttt gatgaaaaaa cgatgtatca agcggcatat
    11581 gcgtttgaac aagcaacaga attccataaa caacaaccag ctattttagg agggaaagac
    11641 gaatgaattt tgaaacagtc attggactag aagtccacgt cgagttaaaa accaactcaa
    11701 aaattttctc tccagcgcca gctcattttg gtgcagaacc aaacagcaat acaaacgtga
    11761 tcgactgggg ttatccaggc gtgctgccag taatgaataa agctgcgctg gaattcggga
    11821 tgaaagcagc tttggcatta aactgtgaaa tctctaaaga tactcatttt gaccggaaaa
    11881 attactttta cccagataat ccaaaagcat atcaaatttc ccaatttgat caaccaatcg
    11941 gtcatgacgg atggatcgaa atcgaagtag aaggtaaaaa gaaaaaaatc cggatcgaac
    12001 gtgtccattt agaagaagat gctggaaaaa acatgcatgg tattggcggt tattcttacg
    12061 ttgatttgaa ccgacaaggc acgcctttga tcgaaatcgt ttctgaagct gatatgcgtt
    12121 caccagaaga agcatacgct tatctagaag cattgcgatc aattatccaa tttacagaag
    12181 tcagcgatgt gaaaatggaa gaaggctcta tgcgctgtga tgccaatatt tctcttcgcc
    12241 cttatggaca agaagagttc ggtacgaaag cagaattgaa aaacttgaac tctttatctt
    12301 tcgtgaaaaa aggtttagct tttgaagaaa aacgtcaagc aaaagttctt ctttcaggag
    12361 gagaaatcca acaagaaaca cgccgttttg acgaaacaac gaataaaaca gttttgatgc
    12421 gtgtcaaaga agggtctagt gattatcgtt acttcccaga gcctgatatc ccgaaattcg
    12481 tgatcgatga cgaatggatt caaaaagtaa aagctagttt gcctgagatg ccagcttctc
    12541 gtcgcgaacg ctatatccgt gagtttggac ttccagagta tgacgcaatg gtattgacac
    12601 ttacaaaaga aatgtctgat ttttttgaag ctgttttgga aaatggtgca gatgccaagc
    12661 aagcatctaa ctggttgatg ggtgaagttt ctgcttactt gaacagtgag aaactggaat
    12721 tagctgagac aaaattgaca cctgagaact tagcaggaat gattcgtttg atcgccgatg
    12781 gaacgatcag ctcaaaaatc gccaaaaaag tcttccgtga attgatcaca aatggcggag
    12841 aagcccaaga agtagttgaa aaaaatggtt gggtgcaatt atctgatccg aacaaactac
    12901 tgccaatcat taatgaaatt ttggataaca accaacaatc tgtcgatgat ttcaaaaatg
    12961 gaaaagatcg tgcggttggc ttcctagtcg gtcaaatcat gaaagcaact aaaggacaag
    13021 caaatcctgg tgtcgtgaat aagctactta aagatgaatt agcgaaacga tagaggctga
    13081 aaatgaaaaa agcacgcgta atttataacc ccacctcggg aaaagaatta ttgaaaaaaa
    13141 atttggcgga tatcctacag gcattagaag aagcaggatt tgaagccagc gcctatgcaa
    13201 ctacaccaga accagattca gccaagaatg aagcatgtag agtcgcagaa ctaggttttg
    13261 atctagtagt ggcagctggt ggcgatggca caatcaacga agtggttaat ggaatcgctc
    13321 cgcttgaaca ccgtcctaaa atggccatca tcccagctgg tacaacgaac gactatgctc
    13381 gagcattaaa aattcctaga gataatatta aagcagcagc agaagtgata aaaaaacacc
    13441 aaaccgtaaa aatggacatc ggcctttctg ataacagtta cttcatcaat atcgctgcag
    13501 gtggctattt gacagaattg acttatgaag ttccatcaga actaaagagt atttttggtt
    13561 atcttgctta tcttgcgaaa ggagcagaga tgctccctcg agtaaagcca atcaaaatgc
    13621 gcttgaagta tgatgaaggg gaatatatcg gaaatgcttc gatgtttttc ctagggctga
    13681 cgaactctgt tggaggattc gaacagatcg cgccagatgc caaattggat gatgggaagt
    13741 tttctttaat cattgtgaag acagcaaata tctttgagat cttgcatatt gcagcactca
    13801 tgctgaatgg cggtcgacat gtcgatgatc caagaataat ctataccaaa acaagcagtt
    13861 tgttcgttga aacagaagat gatatcaatc gaccagtaat gatcaactta gatggtgaat
    13921 acggcgggga agcaccgatg catttccaaa atcttcatca acacattgag ttctttgcca
    13981 ataccgatca gattcctgat gaagcaatca caggtacaga tgaagaagaa ttagaggttg
    14041 tcagcaaaga attcgtaaaa gaagtcgaac gtttaacgga tgaagatatc gacggtgacg
    14101 gaaaaatagc agacgaaaca cctaatcaaa agaattaatc aaaagccggt gctgaacgta
    14161 gtaaggtcag tgccggtttt agttgtgtaa ttagctgatt tatagtagag ttcagtagga
    14221 ttgaaaaata ggcttaaagg agcaaaatat gacagaacag caaatagtaa agaaaaacga
    14281 acgatataca gtcgaaatcg tcgacttgag ttatgaagga atgggggtag caaaaatcga
    14341 tggttatcca ttatttatcg aaaatgcact ccctggagaa gaagtagaaa tactagtagt
    14401 aaaagcaggc aataagtttg gatatggaaa agtcgaaaca tttattacac aatcacctga
    14461 tcgtcagcct gtagaaaaca atgcattact tcgcataggt atcgcacctt tagcccattt
    14521 gaagtatgaa caacagcttg tcttcaaaca aaaacaagta gagcatgtaa tggcgaagat
    14581 tgctaagatg ccagaagttc ctgtacttcc aacggtcgga atggcagaac cattcggcta
    14641 ccgtaataaa gcacagatcc ctgttcgccg tatcgatgga cagttagcca ccggttttta
    14701 caaaaagaat agccatgatc tagtccctat cgaagatttt tatatccaag atcctgtcat
    14761 cgatcaagca atcaaagtag tcagagatat cttgcaacgg ttccaagtac gaggatataa
    14821 tgaagaaaaa aatgaaggac agcttcgtca catcattatc cgtcgtggtc attattccca
    14881 tgaaatgatg gtcgttttag ttactcgtaa agaaaaattc tttaaatcca aggaaattgc
    14941 tgaagctatt catgaagaat tgccagaagt ggtttcggtc atccaaaata tcaatgaaga
    15001 aaaaacaaat gtgattttag gagacaaaga aaaagtttta tatggcagag actatatcga
    15061 agatcagcta ttaggaaaaa cctatcgaat ctcagcaaag tcattttatc aagtaaatac
    15121 tcaacaagca gagtatctgt accaaacagc catcgagttt gccgagttag ataaaaaaga
    15181 cattgtgatc gacgcttatt caggaatagg tacgattggc ttgtctttag cagacagagt
    15241 ggataaggtg tatgggatgg aagttattcc agaagcaatt gaagatgcac agttcaatgc
    15301 tttaaataat aaaatcgaaa atgttcatta tgaagtcgga aaagcagaaa aagttatgaa
    15361 aaaatggcaa gaaaaaggga tcaaaccatc tgtgatcgta gttgaccctc cacgaaaagg
    15421 attagatcat cattttattg aatcagcaat cgaaatgtca ccagaacgaa tcgtctatat
    15481 ctcatgtaat ccggcaactt ttgcaagaga tgctaagtta tttgcagaag caggttatgc
    15541 cgtgaagaaa gttcagcctg tagatctttt cccgcaaacc catcatatcg aattagtcgc
    15601 ttcattcgtt aaaggagaat aaaaatggac caaaaagaaa aaatgaaaga attgatcaaa
    15661 aagaaacaag gtggaggccg atccaaacaa atggaaacac ctaagaatga tcgtaaaaac
    15721 atgagaaaag gccctaaaat ctttaataaa tagacgtgct aaagaaagac taagaagtgt
    15781 taacttttgt tattgtttct tagtcttttt agtggctctt tgtcaataag gactgatggg
    15841 ttgtacaaaa tcaaatctgg gtcagaatga acgtcattct gtctcagatt tttcgttatt
    15901 tcaatttgat taactgattg atcaaaaaga tttttatttt catattctca aatgattcaa
    15961 cactaaatat acatgtttga cgcggaaatt attattcgat tttggcggca tcagggggga
    16021 tgacgacgta gcagacccat tattttactc aagaaaatgt ccgtgcctat tatttagaat
    16081 acgatagtga acgtgctggg agttttgttc ttttagagaa agtctctgaa gataaattag
    16141 ttgtcttaga ttttgactgt aacgaaaaat ggcgaaatgg aagagcacca gcaagtgatt
    16201 gaccggatct acgaagcgac acgatatctg cctttggatc gtttgtgctt aagtcctcaa
    16261 tgtggatttg cttcaacaga agaagaaaat gttttgtttg aagaacaatg gaaaaaattt
    16321 cttttgtcaa aagcattgca gaagaagttt ggggaaaatc attaaatcat ctaggagagc
    16381 ttgataggaa ataacactat ggtataatca gaatgcttaa tactaactac tgagggagcc
    16441 ttatgaaaaa gaaaattctt aaatttatcg gatggttctt cggaatcatt cttgggatcg
    16501 cgtttgcaat ctttcttgcc ttccagcttt cccccagacc tggagcattc ttgatcaacc
    16561 atatgttttc aaatactgtt caaatcactg ataaaaaaac gttcgatcaa gcagaaaaaa
    16621 acgtggcgaa aaaaacagat ttgacgtatg agtccaagta taaagagaat acttatgaca
    16681 tctactatcc aaaaaacagc aaggggcctg ttcctgttct attttgggta catggtggag
    16741 gattcgtcgg cggagataaa tcgggtgtca aagaatttgc gacaaaatta gtttcagacg
    16801 ccaacatcgc agtggttgcg atgaattatg aattagcgcc tgattcagaa taccctaatc
    16861 aagttcttca agtgaatgaa ctgatcaaag atttattagc tgaagaaaaa gacgacaaat
    16921 tgctagacat ggaacaatta ttttttggtg gagatagtgc aggttcacaa atcgcattgc
    16981 aatatgcagc tatacaaacg aatgatgcgt atgctaaaca aatgaaaatc aaacagctac
    17041 ttccaaaaga tagtctgaaa ggaaccattt cttattgcgg acctgttgat ttgaagctaa
    17101 cagcacagca gcattcggat gatcgattta tgaagttttt cgtcaaaacc gttgcgtggt
    17161 cgttgattgg aacaaaagat tggaaaacga gtgagcaatt gaaagaagct agtgtggctg
    17221 accatgtttc taaggaattt ccgccaacgt atataactga cggaaatgca tactcattcc
    17281 aggagcaagg tcaagcatta gaaaatcggt tgacagaatt aaaggtaccc gtgcaatcct
    17341 tgttttatac agatacaaaa aaagagatta cccatgaata ccaatttgat tacacgctaa
    17401 aagaatcgca aaattgttac cagcagacat tggattttgt gaataagtat aagtaagagg
    17461 ttgtgaaaaa gccgtttcgt tttgagtatg aagaaacgaa gcggcttttc acaacctctc
    17521 tttcaatacc tttttgttct tgggataaaa ctttccctaa caatcaatcg tgcaggataa
    17581 gtcaaaatga ttggttcgtc ccgtataccg tcaattcgct ccaatgccat tcgaacagct
    17641 gcttgcccaa ttgatttcgc attgatttga acactggtca agccagggga gaggtttgcg
    17701 attgtctcga tattatcaaa gcttgctaca acaatctctt ctccaatttg gatatttttt
    17761 tgtttcaatg cccgatagac accaattgcc attggatcgc tcccgacaag aatggcagta
    17821 ggaagagggg attcattttc taatagatta gtcgcaagta cctcaccttg tttttctgtc
    17881 cagccaccta gatgataaga catatatttt tctaagtgat gatcgatcat aaaatgctga
    17941 taggcacgca tcctcttttc atttatagaa atctgcttcc caccgaattc atccatatcg
    18001 actttatatc ccccgatata ggctattctt ttatgaccat tttctaagaa caattccagt
    18061 acggttttag tcatccgttc aagatctgca tacaccatat cgacctcttg ctggatgtca
    18121 gggttatcca cgacgactat ttgcatgttt tgttttaaca aggcggtaat cgattctttc
    18181 gtaacagtcc caacaacgat caccgcacct aactgatcta gatcttccca ttgtttagga
    18241 ttatcagata gattatagat tcgattcatc cctaaatgca gacgtcgagc ttcttcttcc
    18301 atccctactc gcaaatgttt gtaatagata tcctgttctt cgctcgcttt gccgatcgta
    18361 gagatcacac caatcgaagg cattctcttt ttgatatatt tacgagtact gatgtcataa
    18421 tccaacgcag cagctgcctc tactatccgt tgccgagtag tctgagccac atggatcgtc
    18481 tgatcgttat tcaacacacg ggaaactgta gcaggagaga catttgcctt tcgtgcgaca
    18541 tctctaattc caaccatttt tacacactcc tcaaaaaaat aaaaattttt tattgacaaa
    18601 attgtttagt aaacagtata caggaattat aaaaaaaagt aaacggtttc cgttttgttg
    18661 ggagtgaaat gatggatatt acgcatagaa aaacagataa aaatttcatc ttatttgatg
    18721 aaaagagccg agtcttccat ttacgaaata catttctttc ttatctgata aagatagaag
    18781 aaagcaatgt attggcgcat atttattttg gcaaaccagt caagcagtac aaagacaata
    18841 aaaattatcc ccgaagagat cgtggatttt caggaaatgt tcccttaaat cctgaccggt
    18901 ctctgtcgaa agatacacta ccgcaagaat actcttctca tggcagcatg gactttcgaa
    18961 cgcccgcatc gatcattcag cgcaagaatg gatcagactt actggatctg agatatgaca
    19021 gccattatat tacagatggg aagccagaga tcgaaggctt gcctcaaact tatgtgatgg
    19081 acaagtcaga ggcacaaacg ttggtgattt cacttaagga tagagaaaca gcgatttatt
    19141 tcgatttgtt ctataccata tttactgata gagcagtgat cacacgatct gtaaagataa
    19201 gaaatgaaac cggagaaaca atcaaactag aaaaagcggc tagtttccag ctagactttg
    19261 cccatactag gcgatttgat gaagtcattg ctttgccagg agcacatgtc aatgaaagac
    19321 agatttcacg gcaatctgtt ttatctggaa caaaagtatt tgaaagccgt cgaggaacga
    19381 gcagtcacca tatgaataac tttattgctt tagttcatca tcatacgaca gaaaatactg
    19441 gagaagcgat cggacttcaa tttgtttatt ccggcaacca ttctttcgaa cttgaaaaag
    19501 atcagatcaa tcaattgaga gtcgtcggtg ggatcaacag ccatcgtttt tcgtgggaat
    19561 tgaatgcagg ccagtctttt caaacacctg aaatgatcct ttcttactca agtcagggat
    19621 tgaacaaaat gagtcagatc caccatgagt tgctacgtga aagaatcgca agaggtcgtc
    19681 atcaatttgc tgaacggccg attctggtca ataactggga agccacttac tttgacttca
    19741 atagtgaaaa aatcaaggcg atcattgatg aagcaaagga gctaggcatc gaaatgttcg
    19801 tattagatga tggctggttt ggaaaaagag atgcggacaa ttcttcatta ggagactggt
    19861 tcgagtatga aggcaagttg acaaatggat tacgtgaaat agcagattat gctcatagca
    19921 aaggattgaa atttggttta tggtttgaac ctgaaatgat ctcggttgat tctgaactgt
    19981 atcgaacaca tcctgatttt cttatgcagg agccaggtag aatgccttct gcttccagat
    20041 cacaacatgt actggatttt acaaggttag atgttcggca gacgattgag aaacaaatga
    20101 gaaagatttt agatacgatt cctcttgact acatcaaatg ggatatgaac cgttcgcttt
    20161 cagatgtcta ttcgattacc cttgacccac agagacaagg agaagtagcg catcgttata
    20221 tgctcgggct ttatgaacta cttgaacatt tgacaactga ttatccggaa atcttatggg
    20281 aaggctgttc tggtggaggt ggacggtttg atgccgggtt tatttattat atgccgcagt
    20341 catggacaag tgataacacg gatgcagtgg agcgtatgaa aatccaatat ggaacaagcc
    20401 ttgcttatcc gatttcgtcc atcacagctc atgtatcagc tgtaccaaat catcagaccg
    20461 acagatctac ttcgttgaaa acaagaggag aaacagctat gagcgctgtt tttggttatg
    20521 aactggattt gacaaagctg tctcctgaag aaaaaaagca agtaaaagac caaattatat
    20581 cctatcaaac gattcgtccg gtgatccagt atggccatta ttatcgtttg gcgagtcctt
    20641 ttgaagaaaa tattgccgca tggatgtttg tgtctcctaa acaagatgaa gctattgtgt
    20701 ttttagggag gatactagct tcagctcaac cagcgttcca tgaagtatat ctgatggggt
    20761 tagatgatga ggcactttat caggaacaga cctcgaagcg gatattttcg ggggccgaat
    20821 tgatgacagt tggactttac ttccccgatt ttcaaggtga tttccaaaca gaactgcttc
    20881 atttcaaaaa gttatgagag agaaggaaaa aagtatgaaa gcaaatataa aatggttaga
    20941 tgatccacaa gtttttagag tgaaccaatc aaatgcacat agcgatcatg tttgttatgc
    21001 aacaagggaa gaagtagaaa ccacatcctc ccttaaacag tctttaaatg gatcatggcg
    21061 tttttgttat tcgccaaatc cttcggtacg accaaaaaca ttttataaaa aagattttga
    21121 tgcctgttct tttgatgaaa tcactgttcc tagtcatatt gaattagcaa attatgacaa
    21181 gatccattac atcaatacga tgtatccgtg ggagggacat gtatttcgcc gtcccccttt
    21241 cacgacaagc cctgagaaag aggcggaagg gatcttttca gctgctaaag acaatccggt
    21301 tggttcgtat ttgacgactt ttcgattaga agaaggtcta aagggaaaac aaatctcgat
    21361 tcattttgct ggtgtagaac aagcaatgtt tatttggttg aatggtcatt ttatcgggta
    21421 tgccgaagat tcgttcactc cgtctgaatt tgatttaaca ccttatttgg aagaagaaaa
    21481 tattctagct gtggaggttt acaaaagaag tacagctgca ttcttggaag atcaggactt
    21541 tttccgattt tttgggatct tcagagatgt cttcttagtt gctcgaggac aaaaccattt
    21601 agaagatctt tggatcaaac ctcgcttaga tgacaaatgt caaaatggca aacttgaagt
    21661 agatttgtct tttgagcaag tagccaaaaa cagtcagctg aatgtagcga tactagatcc
    21721 tgatgggaag gaaatctatc aacagcaata tccgttaaaa gagcaaatga cgattcgcat
    21781 cgatggattt gaaaatgtgg cactgtgggg gcattttcag cccaatttat ataagcttga
    21841 attggagacg atagacgaag aaaaaagagt aacggagtat attgactatt ccttcggttt
    21901 tcgtaaaatc gaaatcaaag ataaaatcat ctacttcaat ggaaaacgga tgattatcaa
    21961 cggggtaaat cgacatgagt ggcatccgaa aaaaggacgc gccatcacta tagaagatat
    22021 ggaaaaagat ttggctatca taaaaaatgc aaagatcaac ggcgtacgga catctcatta
    22081 ccccaaccag atcacttggt attatttatg cgaccaagca ggaatccatg tgatggcgga
    22141 aacgaacctc gaatcccatg gctcatggca aaaaatgggc gcatttgagc cttcttacaa
    22201 cgtaccaggt gcagtacctc agtggcaggc ggctgttctt gatcgagcaa aaacgaattt
    22261 cgaaacatta aaaaaccatc caagtattct tttttggtct cttggaaatg aatcttatgc
    22321 ggaagaaaac attcgacaaa tgaacgatta cttcaaaaaa gtagaccccg atcgacttgt
    22381 ccattatgaa gggaattttc caaatccagc attcgaagat tcgatttcag atgtaaaaag
    22441 ctgcatgtat gcgccaccag aagagatcat tgactacctt gaaaataaag cggaaaaacc
    22501 gttcatctta tgtgaatata tgcatgacat ggggaattcg ttaggtggaa tggattctta
    22561 catgacgttg cttgatcgtt atgaaatgta ccaaggaggc tatatatggg attttaaaga
    22621 tcaggcgatc caagtgactg atccaatcac aggcagagaa gtgatgcgct atggcggtga
    22681 ctttgacgat aaaccaagtg actatgaatt ctcagggaat gggatcgttt ttgcagatgg
    22741 acaagaaaaa cccgccatgc aggaggtaag atattattat gaaaaataca gtaaataaaa
    22801 gtcatatgga tacggaaaaa gttgcaatcg tcttcggcga ctgtacatta ggtgtcaaat
    22861 cggggaatac gcattatatt ttttcttata caagaggcgg actggaatcg ctcaataaaa
    22921 acggaaaaga atggctatac cgggaaacac ttccagtttt ttggcgagca acgaccgata
    22981 atgacagagg tagcggattt ggatttaaat ccggtatgtg gttaggagcc ggattgtatc
    23041 caaaagtcaa agaaatagaa gtactggtgg acaataaaaa gatcgatttg ccgattgctc
    23101 cagtaaataa tcaatattca agtaatgaat acgctgaaac ggttgaaatc atttttacac
    23161 tggaaatcaa cacagtacca aaaacccaag tgacaatttc ctaccaaatc gctggagagg
    23221 gtaaaatgct tgtcaatatg cattattatg gacaaaaagg attaccggaa ttacccgcat
    23281 tgggtttaag gttcattatg ccaacgaaag caaaattttt tgagtatgaa ggcctatcag
    23341 gggaaacata tccggatcgg atggctggtg gtgtcgaagg tatttataaa gtggcaggac
    23401 tccccgtttc cccatacctt gttccccaag aatgcggcat gcatatggaa acgaaatggg
    23461 tagaactaac aagaaatcaa acgttggata atacggataa agatcaagaa gatttttcgt
    23521 tgcgatttga acagcaggat acgccatttg ctttttccgc tttaccattc aaagcagaag
    23581 aactggaaca ggcaacacat atagaagaac taccgccaga aagacgaacg gttcttacga
    23641 tttttgcaaa agtaagagga gtcgggggta tcaatagctg gggagcggat gttgagtcac
    23701 cttaccgaat cagcagcgaa gaggaccatg aaatcggctt tattattcag tagcaatctt
    23761 tagattatta tttgaaaaac ggagtttaca agacaaagct gctttattct atatgtcatg
    23821 aacaagagag taaaaaagtt ctcagatact tttgttcatg actattttct tcaataaaac
    23881 caattgttat ttttctgaaa aacaaagtta actatcagaa aattaaaaaa agtttcataa
    23941 aaa
//
LOCUS       DAGCYL010000022        23883 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.022, whole genome shotgun sequence.
ACCESSION   DAGCYL010000022 DAGCYL010000000
VERSION     DAGCYL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.022"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(53..787)
                     /locus_tag="MB216_000702"
     CDS             complement(53..787)
                     /locus_tag="MB216_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBT4525560.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="MB216_000703"
     CDS             1114..1524
                     /locus_tag="MB216_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBT4525561.1"
                     /translation="MNFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="MB216_000704"
     CDS             1517..2218
                     /locus_tag="MB216_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBT4525562.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="MB216_000705"
     CDS             2218..3804
                     /locus_tag="MB216_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4525563.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="MB216_000706"
     CDS             3823..4044
                     /locus_tag="MB216_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBT4525564.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="MB216_000707"
     CDS             4041..4802
                     /locus_tag="MB216_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBT4525565.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="MB216_000708"
     CDS             5182..5691
                     /locus_tag="MB216_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBT4525566.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="MB216_000709"
     CDS             complement(5766..6305)
                     /locus_tag="MB216_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBT4525567.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="MB216_000710"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="MB216_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBT4525568.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="MB216_000711"
     CDS             7318..8169
                     /locus_tag="MB216_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBT4525569.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="MB216_000712"
     CDS             8197..8832
                     /locus_tag="MB216_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBT4525570.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="MB216_000713"
     CDS             8852..9625
                     /locus_tag="MB216_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4525571.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="MB216_000714"
     CDS             10033..10830
                     /locus_tag="MB216_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBT4525572.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="MB216_000715"
     CDS             10808..11221
                     /locus_tag="MB216_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBT4525573.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="MB216_000716"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="MB216_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBT4525574.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="MB216_000717"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="MB216_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBT4525575.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="MB216_000718"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="MB216_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBT4525576.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="MB216_000719"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="MB216_000719"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBT4525577.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="MB216_000720"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="MB216_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBT4525578.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="MB216_000721"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="MB216_000721"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBT4525579.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="MB216_000722"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="MB216_000722"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBT4525580.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="MB216_000723"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="MB216_000723"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBT4525581.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="MB216_000724"
     CDS             21905..22483
                     /locus_tag="MB216_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4525582.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="MB216_000725"
     CDS             22535..23017
                     /locus_tag="MB216_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBT4525583.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="MB216_000726"
     CDS             23134..23508
                     /locus_tag="MB216_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525584.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7807 a         4289 c         5124 g         6663 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaatt tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttttt gagagtataa aatattttgt gtaaatgaaa aaatccatac aaaaaaggaa
    23881 gtc
//
LOCUS       DAGCYL010000023        23727 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.023, whole genome shotgun sequence.
ACCESSION   DAGCYL010000023 DAGCYL010000000
VERSION     DAGCYL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.023"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            42..674
                     /locus_tag="MB216_000727"
     CDS             42..674
                     /locus_tag="MB216_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525585.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(808..933)
                     /locus_tag="MB216_000728"
     CDS             complement(808..933)
                     /locus_tag="MB216_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4525586.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1067..1960)
                     /locus_tag="MB216_000729"
     CDS             complement(1067..1960)
                     /locus_tag="MB216_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBT4525587.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2142..3068)
                     /locus_tag="MB216_000730"
     CDS             complement(2142..3068)
                     /locus_tag="MB216_000730"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBT4525588.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3153..3914)
                     /gene="pflA"
                     /locus_tag="MB216_000731"
     CDS             complement(3153..3914)
                     /gene="pflA"
                     /locus_tag="MB216_000731"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBT4525589.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4158..6389)
                     /gene="pflB"
                     /locus_tag="MB216_000732"
     CDS             complement(4158..6389)
                     /gene="pflB"
                     /locus_tag="MB216_000732"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBT4525590.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6666..9116)
                     /gene="parC"
                     /locus_tag="MB216_000733"
     CDS             complement(6666..9116)
                     /gene="parC"
                     /locus_tag="MB216_000733"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBT4525591.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9128..11173)
                     /gene="parE"
                     /locus_tag="MB216_000734"
     CDS             complement(9128..11173)
                     /gene="parE"
                     /locus_tag="MB216_000734"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBT4525592.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11339..11773)
                     /locus_tag="MB216_000735"
     CDS             complement(11339..11773)
                     /locus_tag="MB216_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBT4525593.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11900..12538
                     /gene="plsY"
                     /locus_tag="MB216_000736"
     CDS             11900..12538
                     /gene="plsY"
                     /locus_tag="MB216_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBT4525594.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12670..13545)
                     /locus_tag="MB216_000737"
     CDS             complement(12670..13545)
                     /locus_tag="MB216_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBT4525595.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13627..14418)
                     /gene="codY"
                     /locus_tag="MB216_000738"
     CDS             complement(13627..14418)
                     /gene="codY"
                     /locus_tag="MB216_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBT4525596.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14436..15836)
                     /gene="hslU"
                     /locus_tag="MB216_000739"
     CDS             complement(14436..15836)
                     /gene="hslU"
                     /locus_tag="MB216_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBT4525597.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15850..16398)
                     /gene="hslV"
                     /locus_tag="MB216_000740"
     CDS             complement(15850..16398)
                     /gene="hslV"
                     /locus_tag="MB216_000740"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBT4525598.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16435..17340)
                     /gene="xerC"
                     /locus_tag="MB216_000741"
     CDS             complement(16435..17340)
                     /gene="xerC"
                     /locus_tag="MB216_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBT4525599.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17538..18914)
                     /gene="trmFO"
                     /locus_tag="MB216_000742"
     CDS             complement(17538..18914)
                     /gene="trmFO"
                     /locus_tag="MB216_000742"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBT4525600.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18883..20961)
                     /gene="topA"
                     /locus_tag="MB216_000743"
     CDS             complement(18883..20961)
                     /gene="topA"
                     /locus_tag="MB216_000743"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBT4525601.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21083..21940)
                     /gene="dprA"
                     /locus_tag="MB216_000744"
     CDS             complement(21083..21940)
                     /gene="dprA"
                     /locus_tag="MB216_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBT4525602.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21996..22763)
                     /locus_tag="MB216_000745"
     CDS             complement(21996..22763)
                     /locus_tag="MB216_000745"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBT4525603.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22756..23616)
                     /gene="ylqF"
                     /locus_tag="MB216_000746"
     CDS             complement(22756..23616)
                     /gene="ylqF"
                     /locus_tag="MB216_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBT4525604.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         6176 a         5117 c         4075 g         8359 t
ORIGIN      
        1 tattatactt tatatactta tcaatttaat ttggagatat tatggaaatt caacgtagaa
       61 ccactaattt tttacattta ggctcttttt ttgcaacttt gacgatctta ttttttctat
      121 gttattcata ccttaagacc tcacatgcgc cttctagtgc attaggctgg ttgcttttgg
      181 atcattctga tatcacagaa gccgaagaaa atccgttttt tttgattttg tcttctcttt
      241 gcggcttact gttttctgtt tatttcggtg cctatttttt tcatcgacat cctggtacgt
      301 taaacgaagc ttcaagcgta ttgattgcat ttaaaacgtt ttctttttta gcaattcttt
      361 tttattcgtt taatcacaat acgcttttct tctttatcat gaatggttta gtcgtgattt
      421 tatccatttt tacttatttt attttttcac tcatttttag ccaattgaaa tgtccgctct
      481 tctttcgatt agttcctgtc acctgtttta tttacagctt gttttccttt tttgtccttg
      541 ctttaatgcc actgaccaac ccttcgacga tccaagtcgc aggaaatctg ttggatatgc
      601 tcttcatcgt cagtctaggt gtatttatgt ggcaaagaaa aaacaaacaa aataaaaagc
      661 attttgaagc ctgactatta cgacttcata aatgcttttt tagttttaaa cacttcaatc
      721 taaaagaata acccaagatt ggataaatta ttaatgactc gccattcgtt tcttagtgat
      781 tctggattat caaatggact tcctaatcta atcagctagt tcttcccata aaatgccata
      841 cttgactgaa tcataatatt gattatttaa aaatctaact tttcttatca ctacttcctt
      901 tgtcattcca catttctcac catttttttg cattcctttg tttccagacc atgtagtgaa
      961 tctaataggt ggtagatagg caaaatactc aaataattct tttaaccata aaggaagaga
     1021 aaggctcctc ctgcaagagt cagtataatt tttgcttact gctctcttag gttttactca
     1081 ctaaaactgt tccatattct ttgatataag aaaatgcatg cggtataaat ttcttgctca
     1141 gctcagaaaa gatttgtcgt ttaagaaagg ccatttcctc cattgtcaat gaaggatcca
     1201 atgttggtaa ttcaatctca aaattgtttt tttcgacaat aggtaattct aatgtgaatg
     1261 attcttcttc ctttatagta ttcgtcacaa gtagagactg atggaaaata ctaggccggc
     1321 gatttttata tttattctta cgtatcctgt caacgataac ttctatttga tcaaaaaagt
     1381 acgattcatt tacgtctcca ataagttcgg ctaattcttt gaatgccttc gttacaggaa
     1441 gctgattctt ctgtacaaaa aagtaattag ccaaagtaag cgggaagtgt tctatagagg
     1501 aaagtgtgat aaaacggtac ttctcatttc ctgcagttgt cctcagcaat tctgtctctc
     1561 ggctgtttct tactccataa aaatacgctt ctttccgttc atcttcgaaa caatacgccc
     1621 aaataacttc tcccatcgct aatttttgtt catcaacaga gagtctcttc aattgttcag
     1681 ctatggtttc agcagccgaa gtggcttgtt gcaagtaatc tttaggatca aatattggaa
     1741 agttcaaatg atatcttcgt tcttccctct tgattaatcc tgcatcgatc aataaatcaa
     1801 gaaaatggga aaatttattt tgcgaaaatt cttttttcaa ttctcttaga atcgtttctt
     1861 gttctcgatg ttcatataga taatcggtca atgaagaaaa aagcggatca tcggtcagtt
     1921 tttgataaaa gttttcctta ttttttgaat aatagttcat atatgttctc ttttctatcc
     1981 gttttatttt acttaaaagg cttttacgta aaaaggagga agaacaagag tcataattga
     2041 ctgttattct cctccatttc ttcggccttt agtatgttct atgtttcttt ttatatcaat
     2101 cgcttatcca ttcattttga atgcttcata ctaccatttg attagctaaa tacagccgtc
     2161 aattgaggta cgacttgttt tttacgtgaa acgacacctt taagcaaagc acggttgttg
     2221 gtcaaagttg tttggaaagc ttcttctact ttttcttttc ctttaccagc aacaagtaat
     2281 tctgaatcac tgtctaagat gtttgtgaca actaatacga acagatcgta gccatttgct
     2341 ttcatttcat cttccattgc tgcttctagc tctgcttgtc gatccatcac ttcattaagg
     2401 tccactgtat tgacttgagc gatacgaacg ttggaactgc ccattgggaa actttttgca
     2461 tccaaatcaa gaagaaccgc agctgattta tcacttagat ttgttcctgc tttcaacatt
     2521 tcaagtccat aagtgtttag atcgacttca gcgattgctg caagttcttt tgcagctgct
     2581 tcatcttcag gcgtacatgt tggtgatttg aacaacaatg tatctgaaat gatagctgag
     2641 agcatgattc ctgcgatttc tttaggaatt gttacttggt tttctttata tagttttaaa
     2701 ataatcgtac ttgtacatcc aactggttct gcacgatagt acaatggatt agctgtttca
     2761 aagtttgcaa tacgatggtg gtctactaca gctaaaatat ttacttcgct gatatctgaa
     2821 atactttgtt ggaattcatt atgatccaca agcatgacat ctgcattttc tgctttagct
     2881 gactctacta ctcgtggtgc agatagctga aagtggtcta atgcgtattg tgtttcttca
     2941 ttaggtgtac ctagagcaac tgcttctgtt tctttaccta attcattttg caagtgtgaa
     3001 aacgcaatgg ctgccccgat tgcatctgta tctggatttt ggtgaccaaa aactaaaatt
     3061 tttgacatgt cattcgctcc tttgtgattt ctcctattta ataatagcaa aaaaacggaa
     3121 agaagcaata cttatcttct ttccgatgcg acctatcgcg ttaaatattt tgtatactct
     3181 tctgtatgaa gcaatcgttt agcattttcg acacgttctt ttgtaggtgg ttcgatccct
     3241 tctaatggat atttcaaacc aagtgtttcc cacttgtatt ttcccatcgt atgataagga
     3301 agaatctcta ctctgtctac atttgacaag gtttgaataa agttgtctag tcggatcaaa
     3361 tattcatcat aatcacttcg ctcaggaact agtacatggc gaatccaaac cggtttgttg
     3421 atttttgaca aatattgagc catttctaaa atattttcat tgtcccaatg cgttagtttt
     3481 ttatgctctt cattatcgat gtgtttgatg tcaaataaca gtaaatctgt atacttcatc
     3541 aattcttgaa aacgtgaaaa gaaaggttct tcatacgtaa aaggttttcc gcatgtatcc
     3601 aatgtagtat gcatcttccg ctcttttgct tttttaaata gttcgatcaa aaaatcgatc
     3661 tgcaatagag gttctccacc actaacagta ataccacctt tatctcccca gtaaggtctg
     3721 aagcgttctg ctttatctaa aagttcatct gctgtgtatt ctttccctcc gccaatattc
     3781 caagtatctg gattgtgaca aaattggcaa cgcatgcggc atccttgcat aaacacgaca
     3841 aagcgcagtc caggtccgtc tactgaaccg aaagtttcga tagaatgaac atagccgatt
     3901 gttttttctt ccatttaagt tcacatcttt tctttgattt cattttaagt acaagttagc
     3961 tgtcgaagcg aagctttctg aacaaaattt ttcgcaattg ccgcttcgtt atgtgtaaaa
     4021 tttacaggga cggaacatac aagtcctgcc cctgtaaatc gtttctttca aataagcata
     4081 ctgattattc gattttgatg cgatggctat tcaacgtcct ttcgacgcta agtcgaaaaa
     4141 cttttttcac gaacttttta cattctgtcg tgtgaagttc tcatcaagac atctaattgt
     4201 tgttctcttg tcaagtcacg gaatttaact gcataaccag atacccggat cgtcaagttc
     4261 ggatatttca ttggattttc cattgcatca cgcaataaat cattactgaa tacattgaca
     4321 ttcaaatgat aagctccttt tgagaagtat ccatctaaga cattacgcaa gttttcgatt
     4381 cttgtatctt gttcttttcc aagacctgaa ggattgattg tttgggtatt tgaaattcca
     4441 tccaatgcac agccgtaatt cattttagct gttgagttca ggctagctaa taacccactt
     4501 tgttccgcat tgtaacttgg atttgctcct ggcgcaagtg gttttccagc ttcacggcca
     4561 tcaggagtat ttcctgtatt tttaccgtaa acaacatttg aagtaatcgt caataatgaa
     4621 gttgttggtt ttgagttgcg gtaagtttta tgacgtttga tttgtcccat gaagtattcc
     4681 aagatccatg aaactaattc atctgcacgg tcatcatcat ttccgtattt agggaagtcg
     4741 ccatccactt caaaatcaac tgctattcct ttttcattac ggattgcttt tacttttgca
     4801 tatttgatcg ctgacaagct gtctgccgca tgagagatcc cagcaatacc tgtagcaaat
     4861 gtgcgttgca agtcagaatc catcaaagcc agctgagctg cttcataaga atatttatca
     4921 tgcatataat gaatgatgtt caacgtattg acatatagtt ctgctaacca atccatcata
     4981 ttcatgaagt tatgtttcac ttcttcaaag tctagtattt ctgatgtgat tggacgatat
     5041 tctggtccaa cttgggcgcc agtcacttca tcgactccgc cattgatggc atataatagt
     5101 gttttagcca agttagcacg agcgccaaag aattgcatgt cttttccgac aactgttcct
     5161 gatacacagc aagcaatcgc acaatcatca gaaccccatt gttcacgaag ctgattatca
     5221 ttttcgaatt gaatcgaaga actttcgata gcgattctgc tagcatatgt tctgaatcct
     5281 tctggaaggt gttcagaata caaaacagtc aagtttggtt ctggtgaagg tcccatattt
     5341 gttaatgtat gaagcagacg gaaatcattc tttgtaacta atgaacgacc atctaatccc
     5401 atacctgcaa tggataaagt agcccagata gggaatcctg agaataattg gttatattca
     5461 ggtgtacgcg cgaatttgac catacgtaat ttcattacta agtggtcgat caattcttgt
     5521 gcttcttttt ctgtgattac tccattatcc aaatcacgtt ggatatagat atcaaggaat
     5581 gctgagatac gtccgattga catcgcagca ccattttgtg atttgattgc tgctaaatat
     5641 ccgaagtata gccattggac agcttctttt gcatttgcag ctggtttaga aatatcatag
     5701 ccatatttcg cagccatttc tttcatttgc gctaatgcac gatattgttc agtgatttct
     5761 tcacgtaaac gaatcacttc ttcactcatt gtatgataac cagtcatatc atgatctttt
     5821 ttcttttgtt ccatcaaata gtcgattcca tacaatgcca cacgacgata gtcaccgatg
     5881 atacgtccgc gaccgtaagc gtctggaagc cctgtaatga ttttgttttt acgagcagca
     5941 cgcatttcag cagtataagc gtcaaataca ccttgattat gtgttttgcg ccagtcagtg
     6001 aaaatatgtg tcaattcatc gtttggtgta tagccattgc tttttagcgc attgttagcc
     6061 atattgattc cgccgaacgg catgaatgct tgtttcaaag gtttgtctgt ctgtagaccg
     6121 acgatttttt ccaaatcttt atccaaatag cctgcatcgt gtgaagtaat agttgatacg
     6181 atttctgtgt ccatatcata gacaccgtta cgctcgaatt ggatatcatt taacttttgc
     6241 aattcatccc ataatttttc tgttgcttct gttgggcctt ccaaaaagct gtcatcaccg
     6301 cgatattcag tgtagttatt ttgaataaag tctctagtgt ctacgccttc tttccatttg
     6361 gtacctttaa atccattcca ttgctccatt gtatggacct cctaaatttc tgttttatgt
     6421 aacagttttt acgtgattag tataacacag caccctatct ttgcaagggt tttcttgttt
     6481 tttatttaac aatttttatg ttttatttac atatatttaa atcaacgttg acataccaac
     6541 atttaagcat tgtgaacact ttcatctttt tttgcaaacg tataaggatt tattaaaaat
     6601 gaatgaaaaa gagacgccac ctgtattaat acagatggcg tctcttcatt aaaatactac
     6661 aacagttacg actcgatgaa aatctcagga gcatcgataa cttcgatgac ttctccacct
     6721 tttttctcat ccatcgcaaa tgatccattc gatgttcgat cacctatagg gaaatcagtt
     6781 gggtcaatcg ttaactgctg ccctttttga ttcatcaagg ttatttcttt tgatcttcct
     6841 tccgacatat agataatacg gtgcggattt tttttcagct cacgcatgac cgtcactccc
     6901 cgcttcgcac gtgaagtttg gctgatttct tgagcaagca tgcgtttaac agctcctcgc
     6961 tgagtaacga ttacgatcgg cgtatcacct tctgctaaca caagcagccc gttgataatc
     7021 ctatctccat ctttcaaatt catagacttc actccagctg cctttgctcc aacaacggga
     7081 acatctgtta aaggatagcg caaacccatt ccttgatgcg ttactaagaa aacatctaag
     7141 cctggctgat cttctttcag atacacagca atcagttcat cttcttccgc ctttagcttc
     7201 atacagctga gcggacggct cttataggtg cgccatggtt cgaactctgt cattcgtgtt
     7261 tgcttgatca atccattctt gctgatgaat acaaatgttt tctctgcttg aattttttga
     7321 tatgggaaaa cagctaaaat cgattcgtct acagacagat tggagattgt ttgagaaatg
     7381 tgctcaccag catctttcca tttcaaatcg ggtagttcat ggacagggcg gtagatcaca
     7441 tttgctttat ttgtgatcag aagaacatgg tccaatgtac tcaattcacc tgagtatagc
     7501 aagtagtcgc cttctcgcat cccgatttct tctggtttgg aagcactata agaacgaagg
     7561 cttgttcgct tcacatagcc ttctcgcgtc acactgacaa ttacgtcttc ttgtgccact
     7621 aagacttgcg tatcaatttt gatttcttcg atttcgtctt cgattgtcgt tagacgagcg
     7681 gaagaataat tttttttcac ttctcgcaat tcttttttca taacagagaa aagctctttg
     7741 tcatttgaaa ggattttatt caactctgtg atttgcgcaa tcaagctttc ggattccttt
     7801 tgaagctctg tgatatccgt gttcgtcaaa cggtataatt gcaatgtgac aattgcttct
     7861 gcttgttctt ctgtaaattg aaagacgtcg actaaattct ttttggcgtc ttttttgtct
     7921 ttactttcac gaatcgttgc gattacttca tccagaatcg ataaagcttt catcaatcct
     7981 tctacgatat gctgtctttt ttgagctttg gctaaatcga attgactacg ttttgttatg
     8041 acttgctttc gatgctcgat ataacttctt aaaatgtctt tcagtccaac ttgatgcgga
     8101 gtcatatggt cgatagcgac catattgaaa ttgtaattga tctgtagctc agtgtttttg
     8161 aacaaataat tcaaaattcc ttgagcattg gcgtcttttt tcaattcgac gacaatttgc
     8221 agacctgttc tatcactttc atctcgtact tcagctattc catcgatttt cttattcaaa
     8281 cggatctcat ccattttttt caccaaggtc gctttattta cttcatatgg gatttctgtg
     8341 atcacgattt gctgtttatt acccttcatt gcctctatct ttgttttcga acgaagaata
     8401 acttttcctc gtcctgtttc ataagctttt ttgatttcat cttttccttg caagatccct
     8461 cctgttggaa agtcaggtcc aggaatatat tccatcaatt tttcaaggct cgcttgtgga
     8521 tgatcgatca tatagattgt gccatcgatc acttcagcta agttatgagt cgggatttct
     8581 gttgcatatc cagcagatat acctgtcgat ccattgacga gaaggtttgg gtatcgagct
     8641 ggaaggactg ttggttcttt ttctgtatca tcaaaattcc acacgagatc gactgtttct
     8701 ttctcgatat ctgctagcat ctctccactt aacttggata atctggcttc agtataacgc
     8761 atcgcagcag gtggatcacc gtccatactc ccgttattcc cgtgcatttc gatcagtact
     8821 tcccgcaact tccagtcttg gctcatgcgg accatcgctt cataaatact gatgtcacca
     8881 tgaggatgat aattccccat gatatttccg acagattttg ctgatttacg gaatcctttg
     8941 tcatacgtat tgccatcttt gttcatggca aataatatcc ggcgttgcac tggcttcaat
     9001 ccatcacgga tatcaggcag tgcacgttct tggataatat atttggaata acgtccgaag
     9061 cggtcaccca tcacttcttc taaagttaat tcttgtactt cttgtcgatt ttccatcagg
     9121 tgacctccta ttctacttca aataactgat tgttttcatt tttatctgct cgttcttcat
     9181 ctaataaatc attagaaact gaaggagaga tttcagtatc ctctttttta tctaagatac
     9241 tgccatcttc ttcgagcgta aattgcacgt gattttcgat ccattttctt cgaggttcta
     9301 ctttgtcacc catcagtgtg gttactcgac gttcagcttg cgcagcatca tcgatacgga
     9361 cgcggatcaa cgtacgggaa gtcggatcca tcgttgtttc ccacaattgt tccgcattca
     9421 tctctccaag tcctttatat cgctgcagca tatatccttt gccgactttt ttaatcattg
     9481 ctgccaattc gtcatctgtc cacgcatatt caatgacttg cttctttcct tgtcctttgg
     9541 atactttata taaaggcggt aacgcgatat atactttccc agcttcaatc agcggtttca
     9601 tataacgata aaagaaagta agcagtaata cttgaatatg ggcgccgtct gtatccgcat
     9661 cggtcatgat gattactttg tcgtaattac agtcttcgat agaaaactca ggaccgactc
     9721 ctgcaccaat cgtatagatc atcgtattga tttcttcatt tttcaagata tcttgcattt
     9781 tagctttttc ggtattgatg acttttcctc gtaaaggtag aattgcttgg aactttctgt
     9841 ctcttccttg tttagcagat ccgccagcag aatccccttc gactaaatac aattcatttt
     9901 tcttagggtt tcgggattgt gcgggtgtca attttcctga aagaagagat tcgccttttt
     9961 tacgcttctt cccatttcgg ctttcttctc tggctttacg tgctgcttct ctcgcttctc
    10021 tggctttgat tgctttacgg accaacatct ggctcatttc gctattttcc tgcaaataaa
    10081 agcccatttg ttcactgatg acgttatcta ctacggtacg agcaactggc gtaccaagtt
    10141 tttcttttgt ttgcccttca aattgcagaa gattttcagg tacacggata gaaagtacag
    10201 cagctaaccc ttctctgaaa tcactacctt ccagattctt gtcccgttct ttcagcaaac
    10261 caacttttct cgcatattca ttataagatt tcgtcatcgc cgctttcata ccagcttcat
    10321 gggttccccc atcttttgtc cgaacgttat tgacaaaaga taaaacattt tctgaataac
    10381 cgtcattgta ttgataagcg acctctactt cgataccttc tttttctcct gaaaaataaa
    10441 caactggtgt caatgtatct ttttcttcat tcagataatc aacaaattct ttgattcctt
    10501 cttcgtaatg aaaaatttcc ttgactggtt cttctccacg aagatcagaa agagagattt
    10561 taacgccttt tagaagaaag gcagattctc ttaaccgttc ggctaaaatt tcatatgaaa
    10621 atttcgttgt actgaatatt gtatcatctg gtaaaaaatg gacagtcgtc ccgtttgctt
    10681 tttttgtttt tcctatcttt ttcaaagtac cgtcaggttt accgccgttt ttaaatttct
    10741 gctgatactc tacgccatct ctaacgatcg ttacaattag ccatttcgat aaggcattta
    10801 ccacactggc tcctacaccg tgcagtccac cagaagtttt gtaccctcct tgaccaaact
    10861 ttccgccagc atgcagcaca gtaaaaatca cttcaactgt tggtattccg gaagcatgca
    10921 ttcctacagg catcccacgt ccgctatctg caacagttat actattgtct tcatggatgg
    10981 tcacatcaat ctcactgcca taacctgata atgcttcgtc aactgcatta tcgacgattt
    11041 catagactaa atgatgcaag cctcggctgt cagtggatcc aatgtacatc cctggtcttt
    11101 ttctaactgc ctctaatcct tccaatactt ggatggacga gtcattgtat tcgttgttta
    11161 ctttttttgc caaggcaaac gctccttcaa taatactttg cggcaaaacc gcaccacgat
    11221 tatcatacta gaaaaggtcg tgaaaaaaaa gagggtagac gagaaatcaa ttttaattat
    11281 ctcctaaccg ctttttcgac attcccctct atcgatcatc tcttctttgt ggtcattttt
    11341 atttttggtt ttctttcaac ttaccaagtt caatcttaat acaacgatcc attacgatat
    11401 ctgtacgtcc tgcttttctt agcatctcag ctgcttcttt gttttcaatc cctaattgtg
    11461 cccagaacac tttagcatca gttttcaaaa aatcacctgc tatttcaggt aaaaattcac
    11521 tccgtctaaa tacatctacg atatcgattg gatcatcgat ttctgtcaaa ttcgcataga
    11581 ctttctcgcc tagaatttct tcaccagcca ataatggatt tactggtatg attttatatc
    11641 cattgtcttg caatacctgt gcaaccatgt aacttgttcg ttctggttta ttgctcaagc
    11701 ctacgacggc aatatttttt gcttcttcca aatagttgaa aatcgtttct tgtgttggat
    11761 tttttacaga catttctgcc accccttgct tttcttttta ttccatctat tatacttgta
    11821 tctttcattt tttcaattgt tcctgttacc tattcatgct aaaataaagg atagttttca
    11881 acttgaaaga gggtaaaaaa tgaaattgat cttattactt attttagcat atcttttagg
    11941 ttctattcct tccggtgtct ggattggcaa attgtttttc aaaaaagata tccgacaaca
    12001 cgggagcggt aatactggaa cgaccaatac atttcgtgta ttaggcaaaa cagccgggat
    12061 tgttgtcctg cttatggata ttttaaaagg tacattggct acttgtttgc cgatgatctt
    12121 ccacctttct gggattaacc ctctttggtt tggggtatgc gccattcttg gccatacatt
    12181 tccgattttt gctgggttca aaggaggaaa agctgttgcg acaagtgcag gaatgctttt
    12241 aggatttaat cctgtatttt ttgtttattc ttgtatcatc tttatcgtta gtttattttg
    12301 tacaagtatg gtgagtttga gcagtatgat cagtgcagta ttgatcactt tatctacgat
    12361 cgtccttcct tatgtagctc ctgtgatttt ggctaagccc aattggttac tgacgatcat
    12421 tgccttttta gtttcttcct ttatctttta tcggcataaa gacaatatca agcgtatcag
    12481 aaaccatact gaaagtcgaa tcccgtttgg tttgaatagc agcaagaaaa aatcctgata
    12541 ctttttcatt ccaaagagat aaaacgggag tgtgacaaat cgatgtcaca ctcccttatt
    12601 acttacagga tagctttttt acacaccttg gtaaacggtg ttccctgcta acttctcggt
    12661 cacaacctct tacttcactg tgatggaata ttttgtcttg aagatttcat gggcacctag
    12721 ttgattgatc cctacttttt ccatcaagtt tccactactg ttcaccgtgt cagcgattcc
    12781 gcaccacggt tcgatacaaa cgaaaggcgc ttcttttgga taaggagacc agatccctac
    12841 ataaggcata tctttatagc ttaaagttac gctatgggaa gatttttcac ttcgaatggc
    12901 gtacgcattt agaccccttg tttcaaaaac caaggcatct tgctcaaaaa gttcacgatt
    12961 caaagccaga ttcgtatttg tctgacctaa cgtcttttga tccatatcga tgaatgggcc
    13021 tttcaatggt aggcgtaatc ttgatttcaa tggagaaaat gccagataga aatcttcaaa
    13081 tgacaatcct tcttcaagag gtacattgaa agcaggatgt ccaccgatag aaaagaacat
    13141 ttctttttca ccggtatttg ccacttgata atgaaccgtg atgccgtctc ctcctaattc
    13201 atagctgatc actaattcaa aatcaaatgg atacttttct aatgtctccg gcgtactcct
    13261 taagatgaaa ctagccattt cttgtccatg ttcaatgact tcaaaatcca tatcccgagc
    13321 aaatccgtgt tgccccattt gatatgtttt cccttgataa acatactgat catcttttaa
    13381 acgtccaaca aaagggaata aaaccggtgc atgtctgccc caatacgctg gatcaccttg
    13441 ccagatatac tctaaaccat gttcttttga tttcagactg atcaattccg caccgtgttc
    13501 agcgatttcg gcgatcagct ggtcattttg aataataaca gtcattgcca ttcctccaac
    13561 ttcgacttac tttttaaaat gggaaggctg gaacaaacat ttctatctgc cccagtcccc
    13621 ttacttctaa taactgtgtt ttgctaactc gtctttaaaa cgggaattca atacttttaa
    13681 ataagttccc ttcatgccta acgaacgaga ttcaatgatt ccagctgatt ccaattttcg
    13741 taaagcattg acgatcacag aacgagtgat gccgatacta tcggcaatat tagaagcagt
    13801 cagacgacct tcatctccat ccaatgcttc aaagatagct tgtacagcct tcaattcgct
    13861 ataagataat gtgttgattg ccatttggac agccgttgca ctccgtacat ctgcttcgat
    13921 gcttcgagac ttctgataaa ggatctgcat accgacaacc gtagctccat attctgctaa
    13981 gactagatcg tcatctgcaa acgattgttc catacgagat aagataatcg tacccaaacg
    14041 ttcgccagct ccaaagatcg gaacgacagt cgtcagacca aatggatatt tctctcttga
    14101 ctctaccgga aatgcagtaa tatcactagt aattgggata ttagcttcag ttttcatcaa
    14161 ctgtgccact gtatcagtat aagattcagg aaattgtttc tcaacaaaca ttgttttgat
    14221 tcgttcattg ttcacatcgt gacgttcatt ataacctagt aatactccgt cacttcctat
    14281 gatataggtg ttgctgtcaa gaataccacc tagtgtgaca gccattttat cgtaaggcaa
    14341 atctgcttgc atgtcaaaag tattcttttg ctgcaaaagt gtgtttatct tgcgggtttt
    14401 cgttagcaag tctgtcattg tcgtcctccg attttttata aaatatagcg acttaaatct
    14461 tcatttgccg caattgagcc aagtttgtca tccacatatg cttctgtgat cgttatttct
    14521 cccatttgca tatctggtgc ttcaaataat agatcttcta gtagtttttc caaaatggta
    14581 tgcagtctgc gtgcgccgat attatctgtg tcacggttga catcgaaagc gatttgagcg
    14641 atccgttcaa ttgcttcttt tgtaaacgta actgacacat tttctgtacc gatcaacgcg
    14701 acgtattgtt ttactaaagc attatttggt tcggtcagaa tcttcacaaa atcttcagct
    14761 gtcaaatcat ctaattctac ccgtatcggg aagcgtcctt gaagttctgg aattagatca
    14821 cttggtttag acaaatggaa agctcctgaa gcaatgaata ggacgtgatc tgtttggatg
    14881 atcccgtatt ttgtgtttac ttgtgaacct tcgacaattg gcaatatgtc tctttggacg
    14941 ccttctctgg atacttctcc agattgctgt gatttagaag tgatcttatc gatttcatcg
    15001 ataaaaataa ttccactttc ttccgctaat ttgattgcct cactatggat atcagcatcc
    15061 ttgactaatt ttgctgattc ttccttgatc aataactcta aagcttcttt gactgttacc
    15121 gtccgttcca cttttttctt cggtgtcaaa gcacctagag tttcatttag gtcgatcccc
    15181 atttgctcca atccattgtt catcattggt gcaggtttct tttggtcttc aacttcaatg
    15241 gttacttcac gactatccag taaacccttt tgtagttgtt ccaagactgt acggcgattg
    15301 attcgaatat cttctgtcac ttcttcttgc ggttcagcgg tattttgctg actagcatta
    15361 aaaagattca tcatttgttc aaattgatta gtggattgtt tctgttcttt tttgatacca
    15421 ggtaccaaga tttttaccaa acgtttttct gcattttttt tagcacttgt gtatacacgg
    15481 ctgtactgct ctttttctac gatttggatc gcattttcta ctaaatcccg aaccattgat
    15541 tctacatctc ttccgacata accgacttca gtaaattttg ttgcttcgac tttgacaaat
    15601 ggtgcattga cgatttttgc caaacgtcgt gcgatttctg ttttaccgac ccctgtaggt
    15661 ccgatcatca gcatattttt tggtgtaata tcttgttgca ttttttcatc taactgcaaa
    15721 cggcgatagc ggttacgcaa agcaacggca acagattttt ttgctgcgtg ctgtcctacg
    15781 atatattcat ctaattcact tacgatttgt cttggtgttt gttttaattc atacatatct
    15841 actcacctct tacagttctt ctacgataat attatgatta gtaaagatac agatatctgc
    15901 ggcaatattt aacgcatttt ctgcaatttc tttcgcactc atgtcgtttt gagcatagtt
    15961 tttcattgct cgggctgcag ccaacgcaaa attccctcct gatccgatag ctaagatacc
    16021 atcatctggt gtgataactt ctcctgtacc tgaaacaaga agcatttctt tatcattcat
    16081 tacgatcagc atcgcttcaa gtttttgcat agattgctgt gtccgccatt cttgagctaa
    16141 ttcgacagct gcgcgttgta gattcccgtt atattcgttt agtttgcctt caaatttttc
    16201 ttctaaagta aacgcatctg caacacttcc ggcgaaacct acaacaactt ctccattgta
    16261 gatacgtcgt actttctttg cagtgccttt catgacaaca gattcaccca ttgttacttg
    16321 gccatctcca gccatagcaa atttcccgtc tttttcgacg gcacagatag tggttgaatg
    16381 aaatggtgat tcgaccatgt ttgtttcctc ctaaatttgt tcttttcctt atttttacgc
    16441 tcttggatgg aaagaacgat agtttttttg taaactttct tttgttacat gggcatagat
    16501 ctgggtagtc gataaattgg catgtcctag caattcttgg actgtccgca aatctgcccc
    16561 attatttaat aaatgagtag caaatgtatg gcgcagcatg tgaggatgga tatcactatc
    16621 caaactgctt tttttgatca tttgattcaa gacatattca attccagtag gcgtgattgg
    16681 ttttccatga tgattcacaa aaacgatatc atgctcttcg tgatattttg tcatcaatac
    16741 ctggcggcct tccctgaaat attcttgcag agcatcttga gcaaaagaac ctagaggagc
    16801 atatctctct ttgttccctt taccatggat caacatgacc tctgaatcaa aatcaatgtc
    16861 tgataccgac aaactgctac actcacttaa acggatgcct gacccgtaaa ggacttccag
    16921 taaagcgcgg ttcctcaaat ccaaaggact atctcctgct acgctgtcaa aaagtgcttg
    16981 catttcattt tcataaaaaa aacgtggcag tctcaaattt ttctttttga gatgaacgta
    17041 agaaaatgga ttttctttta tcacttcttc tttgagaaga tactgataaa acgaacgtag
    17101 gctggctatc ttgcgactga tagaatttct gctatagcta ggacgatgat cagctaatgc
    17161 actcaaatag acacgaaccg taaaatattc cgccttcagc agatcagcgt ctccagattc
    17221 ctccaaaaaa tgtttgaaat ggagaatgtc ttcttcatat gcttctttcg ttttttctga
    17281 atatcctctt tcgacaatca aataacgcaa aaatttttgt tcccacgtgg cttctgtcat
    17341 tttctcacct tccttttgtc ccagagaata atttagcaca gcgtttataa aaagacaaat
    17401 aaattgtcag aatttaacca atatttctaa gtttgttcac aattggcgca attcctcaca
    17461 ttttgtttga gaaattctta agaaaaacgc aagaaaaaga gcgagactat ccgtctcacc
    17521 ctcttcatgg agtttcctta ctcatttggg attgcagttt tttctaattc tgtttttatt
    17581 gcctcattag cttttaaagc tcgttgtgca attgcttcat atcttgcttt tttgtctcga
    17641 atgcgctctg gcagttcagg aaacagacca tagttggcat tcatcggttg gaaatgtttt
    17701 ccttccgcat gagtaatgta gtaagccata cttccaatag ctgtttcttg tggaaactcg
    17761 actggttcca tttcttttgc taagcgtgcg gcgttgattc cagcaagtag tccgctcgct
    17821 gcactttcaa catagccttc tacaccagtc atctgacctg caaagaacag atcttcgcgt
    17881 ttcgttgatt ggtaagtcgg tttcagcaat tctggcgaat tcataaagct gtttcggtgc
    17941 atcacaccat agcgaacgaa ttcagcattt tctaacccag gaatcatccg gaaaacacgt
    18001 ttttgctcgc cccatttcaa gtgagtctga aagcccacga tgttgtataa agaagctgca
    18061 gcattgtctt gacgcagctg gataacagca taaggacgtt tgccagtttt aggatcttcc
    18121 aatccaactg gtttcatagg tccgaaaagc atcgttttga atcctcgctg tgccattact
    18181 tcaattggca tacatccttc aaaatatttt tctttctcaa aagatttaag ttctgccact
    18241 tcggcattga ttagtgcctc atggaaagct ttgaattcct cttctgtcat cggacagttc
    18301 aagtaagctg cttctccttt gtcatagcgg gacttcaaat atactttatc catatcgatc
    18361 gtagctttgt ctacgattgg tgcagctgca tcatagaaat aaaaaccatc tgaaccattg
    18421 aatgctttga tgctttctgc taatggatga gaagtcaaag gtcctgttgc tactacggtg
    18481 attccttccg gtatttcagt aatttcttcg tttatcactg ttactaatgg atgctctttg
    18541 acttttcggg taattgtttc agaaaaagaa tcgcggtcaa cagctaaggc accaccagct
    18601 ggcactgctg tttcatctgc cgaagtgatt actaccgaat tcaatcggcg catttcttct
    18661 ttcaataacc ctactgcatt agccaaactg tttcctctta atgaattgga acatactagt
    18721 tcagcaaaat tacttgtgtg atgcgcctca gtcgatttga ctggacgcat ttcatataaa
    18781 cggacaggga ctcctgcttg tgcgatctgc caagcagctt cacttccggc aagccctgct
    18841 ccaacaacat ttaccatttt caatgaaaga acctcttttc tattattttt ggatgtcttc
    18901 ttcgtaatct ccatttacac agactacttg tttaccgcct ttgactttct tctcgaccag
    18961 atatccgtca cattttggac aatttctgcc cactggttta tcccaagaag tgaattcaca
    19021 ttctgggaaa cgggaacaac catagaatag tctgtttttc ttagattttc gttcaatgac
    19081 ttgtccttca tgacatactg ggcaagtcac accgatttct tttacgattg gtttcgtatt
    19141 ccgacaatca ggaaaattac tgcaagcata aaacttccca tatttcccta attttatcac
    19201 catcggatgt ccacagacat cacaatcgaa tcctgctggt tcatctttga tttgaatttt
    19261 ttcgattttt tcttccgcat tcgtcagttc tttttcaaac ggtttataga aacgatcaac
    19321 cactttgacc cacgcttccg tgccttcttc aattttatcc aaatctcctt ccatttctgc
    19381 tgtgaaatgg atatcgacga tttgagggaa gaattcacag attaatgaat tgacaatttc
    19441 tcctagttca gtcggctcaa atcttttttg tgtcaatttt acatagtagc gtctttggat
    19501 cgtatcaagt gttggtgcat aagtggaagg tcgccctacc ccgttttctt ctagtgcacg
    19561 aatcaacgta gcctcactaa aacgtgctgg tggttgagta aaatgttgtt ttggttcaat
    19621 atcaacagat tggactacat cgccttcttc aagttctgga agaatatttt ctttctcttc
    19681 tttgccgtca tctctacctt ccacatacac ttgcatgaat cctttgaatt tgatttttga
    19741 accgttcgca atgaagatga caccattttg ttctaatgtc actttcattg tatctaaaat
    19801 agctggtgtc atctgactag ccacaaaacg tgaccagatc aatgtataaa gtttcaattg
    19861 atctttatct aaatattgtt tgagttcatc tggtgtacga agcacactcg ttggacgcac
    19921 agcttcatgg gcatcttgtg cgccttgagc atttttgatc tttcggtgat catgtctaga
    19981 aaattctttt ccatacgtat cttcaataaa tgctgcggcc tctgcttttg ctgtatcggc
    20041 aatccgggta gaatcagtac gcatgtatgt aatcaagccg actgttcctt gtttacccaa
    20101 agtgattcct tcatataatt gctgtgctac catcatggtt tttcttgttc gaaagtttaa
    20161 cttacgggca gcttcttgtt gcaggctact tgtagtgaaa ggattcgcag ggtttctttt
    20221 gcgctctttc ttttctactt ttttaacttc gaagtctgtt ccatcaattc tagcagtgac
    20281 ttcttttaca ctttgtgcat ctggtaattt ctttttctta ccgtcgattc cccagaaatt
    20341 tgctttgaat ttcttgcgag cttttttaaa attaccgtcg atgctccaat attcttctgg
    20401 aacaaattct cggatctctt tttcacggtc aataataatt tttaaagcga cagattggac
    20461 acgtcctgca cttaaccctt tcttgacctt acgccagaga ataggactga tcgaataacc
    20521 aacgattctg tctaaggtac gacgagcttg ctgtgcatct actaaatcta catcgatcgt
    20581 tcttggttcc ttaaaagctg ctttgactgc gtcttttgtg atttcattga agaccacccg
    20641 atttttttct ttcagatcca acccaagtag gtacgctaaa tgccaagcaa tcgcttctcc
    20701 ttctctatcc gggtcacttg ccaagtaaac tttttgagct ttttttgctg cggctttcag
    20761 gcttttgatg acatcgcctt ttccgcgtat agaaatataa tggggttcat aattgttttc
    20821 aaaatcgata cccattttac ttttcggtaa atctcttata tggccaacac tggccattac
    20881 tttatagttt cgtccaagat atttctcaat tgtcttggct tttgcaggtg attctacaat
    20941 cactaaatat ttatacgcca ttactgaatg gactcctttc cattgtcgtg ttcctactat
    21001 taaaagagga ttcatacaaa tcgtagctaa tcatatccat tcagaaaacg tttgtcaaga
    21061 aaagcaaatg aaaagtatgg tgttattctt taaattctgc caagatatct tcacccgaaa
    21121 ccgtgcaaac tgcaccgtct tggatcaaat gatgacatcc ggtagaatgt tctgttaaaa
    21181 tatttcctgg aacagaaaaa actgttcttc caagatccaa tgcttgctgt gctgttatca
    21241 gagagccgct ttttttcttg gcttcaaaga cacatgttcc tttagacaaa gctgcgataa
    21301 tccgatttct cattggaaaa caatgctttt tcgggccgga ttgaggagga tattcactca
    21361 aaagaagatg atcatttgca atcacctctt gcaagtcacg attttcagca ggatagactt
    21421 gctctatccc tgttccaatg actgcgattg tccgtccacc atgagagatt gctgcttggt
    21481 gaccgtatgt atctatccct ttcgcgcagc cactgacgat cacatatcca gcttctatca
    21541 ctggttttag taaataataa actgtttttt tggctaaagc agaagtgtgt cttccaccaa
    21601 caacgcttaa catgttacaa gaaagtaaag atagatcacc attataaaac agtacaattg
    21661 gcggataggt caagtgaagc agctgagggg gatatttggg atcaaagcaa gtgataaacg
    21721 actgtttcga ttggattttg ccccagttag cgtgaatttc ctgccaactt ttgcgaaaca
    21781 gctcccggtg tttggttatt ttacctatct taatgatgtc ttcaaatccg atttctttat
    21841 aaggatactg catcatccag ttagcaagtg cccattttcc ttgactactg attccttttg
    21901 tataagcaag tttcaataaa ttttcttcta tttgatacat cataaaaccg cctttcgtct
    21961 ctctttctaa taagatacgt aaaagcggct gttttttatt gtaaatattt ttttacaggt
    22021 gcaaaacttt tacgatggat tggcgtaatt ccttgtcgtt caagtccttc taagtgttcc
    22081 tttgttccat aacctgcatt atgcgagaaa ccatatccag gataaagcac gtcatattct
    22141 ttcatcaatt ggtctcgata tactttagca atgatacttg cagcagcaat cgacacagat
    22201 cgtgcatccc ctttgattat tttttcctgc tgaataggca aatccaaggt catcgcatcg
    22261 atcaacaaat agcttggtac aatggacaat ttttctactg cttggatcat cgctaattta
    22321 cttgcttcat aaatattcac atcatcgatt gtctgctgat caacgatccc gatcccgatt
    22381 gccagggctt tttcttgaac ttgttgataa agaagttctc gctttttctc tgataattgt
    22441 ttggagtcat tcagccctaa tatgatctca tttttcggca agataactgc agctgccacg
    22501 acaggtccag ctaatggtcc tcttcctact tcatcaatgc cggcaatcca ttggtgacct
    22561 tgtgcgtacc cttgcttttc aaagcaaaac atttcttcat atttttcctt aattaatgct
    22621 tcctttttca atcgtcgttc aaaagaagcc agtgcttgct gcacgccttt gcgtggatca
    22681 tttcttaatg attctagtgt aggatcatct attctctgta tgcttttcaa tcgttctttg
    22741 atactttgga tggattcagt catcactttt ttgctccatt tcttcgcaac gatccagtgt
    22801 gtatcgtcca agttttcctt gacgaatatc atgcacgact aattcgctgc cacgatcata
    22861 gtcttcttta tacccacgtt ttgctgtaat atccatcagt aattccggtg ccaatttttc
    22921 ttcttgctcg attgataaac cataacgatc gctgattctt cctgaataat atcgggcaaa
    22981 aaagtctaat ccgtaaatcg ctaaatcatc taaatggaca agctggtctt tgatcgcgcc
    23041 tgtcagcgct agttttttgc cgatttcttc atcttcaaat ttgggccata aaatccctgg
    23101 tgtgtctaac aattccaact cattacctaa acgaagccac tgctgacctt ttgttacgcc
    23161 aggcttattt cctgtctgtg caattttctt gcctactagt cgattcatca acgtagattt
    23221 cccaacattt ggaataccta agaccattgc ccgaatagcg cgaggcttga tcccccgagc
    23281 agcatctctt gcccgttttt ctttcaatgc ttctttagcc gcaggtacta gacctttaat
    23341 tcctttactt tcttgtgccg tgatagctaa tgcaagatgg ccttgtttac gaaaatattc
    23401 ttgccacagc tttgtctgtt gtggatctgc cagatccgct ttattcaata aaacaaggcg
    23461 aggtttttgc tgaatgatct gatctagcat tggattacga gaagataaag gtaatcgtgc
    23521 gtctaccaat tcaaatacga tgtccacata tttcattttt tcagaaactt ctcgtcgggc
    23581 ttttgccata tgtcccggaa accattggat ggtcattgtt ctctccattc ctttcatacc
    23641 ggaatattcc ccggctttac ttgttttttt ctttttcaat ataaattcac ttttttatta
    23701 taaagaaatt ccatctatta gttaagt
//
LOCUS       DAGCYL010000024        22787 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.024, whole genome shotgun sequence.
ACCESSION   DAGCYL010000024 DAGCYL010000000
VERSION     DAGCYL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.024"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            1..369
                     /locus_tag="MB216_000747"
     CDS             1..369
                     /locus_tag="MB216_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBT4525605.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="MB216_000748"
     CDS             549..1490
                     /locus_tag="MB216_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBT4525606.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="MB216_000749"
     CDS             1559..2290
                     /locus_tag="MB216_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBT4525607.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="MB216_000750"
     CDS             2434..4614
                     /locus_tag="MB216_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBT4525608.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="MB216_000751"
     CDS             4634..5116
                     /locus_tag="MB216_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBT4525609.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="MB216_000752"
     CDS             5132..5893
                     /locus_tag="MB216_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBT4525610.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="MB216_000753"
     CDS             6024..7358
                     /locus_tag="MB216_000753"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBT4525611.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="MB216_000754"
     CDS             7665..8150
                     /locus_tag="MB216_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBT4525612.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="MB216_000755"
     CDS             8143..8826
                     /locus_tag="MB216_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBT4525613.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="MB216_000756"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="MB216_000756"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBT4525614.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="MB216_000757"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="MB216_000757"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBT4525615.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /locus_tag="MB216_000758"
     CDS             12442..13071
                     /locus_tag="MB216_000758"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBT4525616.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="MB216_000759"
     CDS             13123..13455
                     /locus_tag="MB216_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBT4525617.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="MB216_000760"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="MB216_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBT4525618.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="MB216_000761"
     CDS             14281..15654
                     /locus_tag="MB216_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBT4525619.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="MB216_000762"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="MB216_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBT4525620.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="MB216_000763"
     CDS             16709..17149
                     /locus_tag="MB216_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBT4525621.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17293..17994
                     /locus_tag="MB216_000764"
     CDS             17293..17994
                     /locus_tag="MB216_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBT4525622.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17991..19112
                     /locus_tag="MB216_000765"
     CDS             17991..19112
                     /locus_tag="MB216_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBT4525623.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19127..20359
                     /gene="pepT"
                     /locus_tag="MB216_000766"
     CDS             19127..20359
                     /gene="pepT"
                     /locus_tag="MB216_000766"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBT4525624.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20491..21399
                     /locus_tag="MB216_000767"
     CDS             20491..21399
                     /locus_tag="MB216_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBT4525625.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21434..21706
                     /locus_tag="MB216_000768"
     CDS             21434..21706
                     /locus_tag="MB216_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBT4525626.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21717..22763
                     /locus_tag="MB216_000769"
     CDS             21717..22763
                     /locus_tag="MB216_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBT4525627.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7677 a         3978 c         5006 g         6126 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata attatctaga
    17281 aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt gggtgacttt
    17341 gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt acccgttgca
    17401 ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca aggtccttat
    17461 gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac cgtacgcctt
    17521 gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac gattgcgggt
    17581 atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca acgcttaagc
    17641 ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg atcttggctg
    17701 caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa aaaaatatat
    17761 gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat ggagctgatg
    17821 ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg gacaagagag
    17881 cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca agaggcacgt
    17941 caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc atgacaatca
    18001 gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta gctgaagaag
    18061 gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga gtaatgatga
    18121 cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga
    18181 ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat cagccacaag
    18241 aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca cacacgaaca
    18301 tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt attgaagtgg
    18361 agagttattt agtaaagact catgagattc attataaaaa actggccgtt tatgtacctg
    18421 tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt acacaaggtg
    18481 attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg gaagctggtg
    18541 cacaaccggc gattgggaaa gtagggaaga cagaacaagt tcaagaggca aaagttgagg
    18601 ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca gctcatcctt
    18661 atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg tttgggttag
    18721 gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa caagtaaaag
    18781 aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga
    18841 agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa
    18901 gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat atgcaaagcg
    18961 ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag caacgattta
    19021 tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt ttcgtttctg
    19081 aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt acgaaaatct
    19141 tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac
    19201 gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga
    19261 gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg gaacattacc
    19321 aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg atacagcaga
    19381 ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg agtctacgat
    19441 cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa atttgaaaaa
    19501 ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag cagatgacaa
    19561 atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc cttctatccc
    19621 tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg gagcggataa
    19681 attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg gtcctttagg
    19741 tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa
    19801 tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag caatcgattt
    19861 tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg aaggtttctt
    19921 ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata tcattcgcga
    19981 tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc aagaagagtt
    20041 gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat attacaacat
    20101 gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag caatgatcga
    20161 gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt caaaaatctc
    20221 ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc acggacgatt
    20281 cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa aaattgcaga
    20341 attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat
    20401 gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt gtcccgccct
    20461 cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt ttataacaaa
    20521 agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa
    20581 aagcagccag aattatatgt agaactagct gaaactggcc catcgattga tgatgcggaa
    20641 ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg tggagacggc
    20701 acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca tgtcggatta
    20761 cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct caaaaaagaa
    20821 gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat
    20881 gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc agtttggatc
    20941 agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt ttttaagtta
    21001 ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca ttggaaagga
    21061 aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac aaattttgac
    21121 gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc cttagatatt
    21181 ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac gatccccggc
    21241 attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact
    21301 cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt atcaaaagga
    21361 ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt aacagaaagt
    21421 tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc ttttcattgt
    21481 gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa ctagcggctg
    21541 gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt tgggaggatt
    21601 gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta taggattcgc
    21661 tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag caggagatgt
    21721 cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt gtgatttttg
    21781 ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta
    21841 ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa atcgctgaag
    21901 ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt
    21961 tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca ggaacgctga
    22021 tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga gcaggggtga
    22081 cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta gctggcttgg
    22141 tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt atcatcgctg
    22201 gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc agcttgaatg
    22261 ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt ttgcggaaat
    22321 gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa accattatcc
    22381 aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt gtattttggt
    22441 atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt ctactgacca
    22501 tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct gctggtattg
    22561 gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca gttgacgcag
    22621 tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc tatggatttg
    22681 tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag
    22741 agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttt
//
LOCUS       DAGCYL010000025        22007 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.025, whole genome shotgun sequence.
ACCESSION   DAGCYL010000025 DAGCYL010000000
VERSION     DAGCYL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.025"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            115..762
                     /locus_tag="MB216_000770"
     CDS             115..762
                     /locus_tag="MB216_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBT4525628.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            846..1790
                     /locus_tag="MB216_000771"
     CDS             846..1790
                     /locus_tag="MB216_000771"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBT4525629.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1925..3376
                     /locus_tag="MB216_000772"
     CDS             1925..3376
                     /locus_tag="MB216_000772"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBT4525630.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3397..4233
                     /locus_tag="MB216_000773"
     CDS             3397..4233
                     /locus_tag="MB216_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBT4525631.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4233..4892
                     /locus_tag="MB216_000774"
     CDS             4233..4892
                     /locus_tag="MB216_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBT4525632.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            5018..5707
                     /locus_tag="MB216_000775"
     CDS             5018..5707
                     /locus_tag="MB216_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525633.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5713..6669
                     /locus_tag="MB216_000776"
     CDS             5713..6669
                     /locus_tag="MB216_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBT4525634.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6713..7225
                     /locus_tag="MB216_000777"
     CDS             6713..7225
                     /locus_tag="MB216_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4525635.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7481..8029)
                     /locus_tag="MB216_000778"
     CDS             complement(7481..8029)
                     /locus_tag="MB216_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4525636.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8118..9299)
                     /locus_tag="MB216_000779"
     CDS             complement(8118..9299)
                     /locus_tag="MB216_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBT4525637.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9535..11043)
                     /locus_tag="MB216_000780"
     CDS             complement(9535..11043)
                     /locus_tag="MB216_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBT4525638.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11202..11393
                     /locus_tag="MB216_000781"
     CDS             11202..11393
                     /locus_tag="MB216_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525639.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11596..12513
                     /locus_tag="MB216_000782"
     CDS             11596..12513
                     /locus_tag="MB216_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBT4525640.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12506..13579
                     /locus_tag="MB216_000783"
     CDS             12506..13579
                     /locus_tag="MB216_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525641.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13727..14245
                     /locus_tag="MB216_000784"
     CDS             13727..14245
                     /locus_tag="MB216_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525642.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14226..14618
                     /locus_tag="MB216_000785"
     CDS             14226..14618
                     /locus_tag="MB216_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBT4525643.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14694..15629
                     /locus_tag="MB216_000786"
     CDS             14694..15629
                     /locus_tag="MB216_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBT4525644.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15762..16541
                     /locus_tag="MB216_000787"
     CDS             15762..16541
                     /locus_tag="MB216_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBT4525645.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16534..16851
                     /locus_tag="MB216_000788"
     CDS             16534..16851
                     /locus_tag="MB216_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBT4525646.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17095..18705
                     /locus_tag="MB216_000789"
     CDS             17095..18705
                     /locus_tag="MB216_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBT4525647.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTIQSTM
                     LQSLNHHRFAIRLTVEAIILKVIFEVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18862..19710
                     /locus_tag="MB216_000790"
     CDS             18862..19710
                     /locus_tag="MB216_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBT4525648.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHVDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19941..20420
                     /locus_tag="MB216_000791"
     CDS             19941..20420
                     /locus_tag="MB216_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525649.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20453..>22007
                     /locus_tag="MB216_000792"
     CDS             20453..>22007
                     /locus_tag="MB216_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4525650.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKVSNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDPDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEFPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPSFQGKTPEVRELHMQVMPIVESLS
                     RQILELLDNE"
BASE COUNT         7214 a         3929 c         4434 g         6430 t
ORIGIN      
        1 tctttatatt ttcataataa aagtttggtt ctttaatttt taaacatgac agaaaatttt
       61 ttcttcgtta tacttgtaat agaatactat tttataacaa aggggatcga atcaatgaaa
      121 aaaactgtta tttatactag tttatttgca gctttgtttt tagctgcttg tcaatcaaat
      181 accaatcaag gcacatctgg gaatacgtct tccagtagcg aacagcgctc ttcacaggta
      241 tcggactcct caacaaaagc aagcacatct tcgtctagcc agacaaaaag ttctagtgaa
      301 gcaagtactc agtcagatat caaagtgtct gcagctcgtg cgatcgaatt gttccaagat
      361 aaatacccag atactgcaat tactagtttg gaattagata gtgattgggg atcttacttc
      421 tacaagatag aaggtgtcga tgatcaaaaa gaatacgaag caagaattga tgctcaaaat
      481 gaaaaaatgg agacagagaa tccagaaact ctagatcgag atgaacaaaa tggtcaaaag
      541 aaaaaagaag atggtctgga tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc
      601 gccgtcgaca aagtaggaag cggtacggca acagattggg atttggataa agatttaggt
      661 actacttatt gggaagtcaa agtaaaagac ggcaatcaaa gtacatcagt caagatcaat
      721 agccaaacag gcgaagtact gtcaactgaa caagatgatt aagaatttct tcggaaaaca
      781 agagttgatt tttcttttag tcatgataca ctagacaaaa taacttgttt gaaggagcga
      841 tttacttgag tttagaaacg acctttgcca atcatatctt tgccaatcct ttgatgaatg
      901 catctggtgt ccactgcatg actacccagg aattagatga actggctcat tcagaagcag
      961 gagcatttat cactaagagc tgtacgatca atgaacgaaa aggaaatccc gaaccacgct
     1021 attttgatgt tcctttaggg agtatcaatt ctatgggact tccaaactta ggtttttctt
     1081 attatctaga gtatgctctt gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat
     1141 ttttttccat tgccgggatg agtgtccagg aaaacttgga aatgcttgag aaaatagaaa
     1201 aaagtgggtt taatgggatc actgaattaa atttatcttg tcccaatgtg cctggaaaac
     1261 cacaactagc ttacgatttt gaggccactt atgaaacact gaaagaagta ttcagcatct
     1321 tttctaagcc gctaggaatc aaattgcctc cttattttga ttttgctcat tttgatcaga
     1381 tggctgatat cttgaatcag tttccattga cctatgtcaa tgctatcaac agtgtgggca
     1441 atggtttgta tatcgataca gaacaagaag cagtagtcat caaaccaaaa gaagggttcg
     1501 gcggcattgg cggagagtat atcaagccga ctgctttggc gaatgttcgc gctttttata
     1561 cacgtttgaa accagaaatc caaatcattg gtacaggtgg gattcggaca ggacaagatg
     1621 catttgaaca tctgctatgt ggcgcaagca tgttacagat tggtaccgag cttcataaag
     1681 aagggccgga aattttttct agaatcataa aagaattaac gcagattatg tcagaaaaag
     1741 gatatacgtc aattgacgaa tttaaaggca aattacgcac catttcatag caaagagacg
     1801 aaggtcatga ttgatcttcg tcttttttta taaaaaacac tcgtctgttc attcctgtgc
     1861 aagcgtactc attgtatgtt agacgaactt gtgggaaaat aacagaggca aaaaggagga
     1921 taatatgaat gatgcaacag aattagcaaa agagcttaaa gaaaaagaat ttagttttca
     1981 agaactagtt acggatatac agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt
     2041 tgtcgcgctt gaaccattcg agagtaattc ccacgagcta ttgattcccc agtcagaaaa
     2101 agggccattg gctggtatcc catttccttt aaaaatgctc ggtcaagaaa aaaagggatg
     2161 gctagctact tcaggatctc gtttatttga aacacaccga gcttctcata catctaatta
     2221 tgtgcggcaa gcagaagcga taggtctagt tccttttgga caaaccaatg cgccagagtt
     2281 tggtttcaaa aatattacag acccagtcat ctatggccca gcaagaaacc cttggaacct
     2341 tgaatgtaca ccgggcggat caagtggagg agctgctgct gcagtttcat caggaatcgt
     2401 tcctttagct ggtgctagtg atggcggtgg ttctattaga atacccgctt cgttttgcgg
     2461 attgattggg ctgaaacctt cgcgagggac gatgccagtt ggtccatatg cttggagagg
     2521 atggcaagga gctgctattg atttcggctt gacagtttct atgagagaca cggaggcttt
     2581 attttatggg atgagaagta tccattccgg tgcgccatat caagtatcgc cagttgagtg
     2641 gcaaacacat ccaagaaaaa aacggttgaa gatagctttt tgtgttgatt caccgatcca
     2701 ctcacagatt agtgaagagg cagtacttgc tgtaaaaaaa gctgtagatt tccttggaaa
     2761 agaagggcat gatttgacag agattcctta cccactagat ggtcgtaaac tgatcacgtc
     2821 ttattatcag atgaatggcg cagaaacagc agcaatgatc cgctcgattg aaacaggcat
     2881 ccagcgagct gtcagaaaag aagaacttga acccatttct tggactttgt tccaatatgg
     2941 tcagaagctc tctgccgcta cttacatcga ttctctgcat gtatgggatg aagcagcaat
     3001 tgtcatggaa aacctacatc aagaatacga tctattcctt tcaccaacga ctgcatttac
     3061 cgcccctaaa atcagcgaag atttgcaaag tgatgcgata agagcacaga tggaacacat
     3121 cagtgattgt tctgaagtag aaggtttaga cgttgtttct tccatgtttg aggagagctt
     3181 gaagatcaca ccttatacac agttggctaa tctgactgga caaccagcaa tcagcttgcc
     3241 tactcatttg acaaaagaaa acatgcctct aggaatccaa tttatggctg ccagaggaag
     3301 agaagatttg ctatttcaaa tcggctatct atttgaagca aatcatcagt tccatttacc
     3361 aaatccatgg aaatagcaga aacaaggaga agaagaatga agaaaaaaag attgtttgct
     3421 ctaataacta gtgccttttt tattttaagc gcttgcggta cagctgccgc taatcaggaa
     3481 gataacagct ggagtaatgt agaaaaaaag aaagaattag tagtagcgac ttctggcaca
     3541 ctgtttcccg catcttatta taatgatcaa aatgaattag ttggttatga tgtagatttg
     3601 gcaaaagagg tggctaaacg gttaaatgtc aaaatctcct ttaaagaata caacgtggat
     3661 ggacaaatca cttctgtttc aaaaagagaa tcagattttg ctgcgaacga ttttggtatc
     3721 acaaaagaaa gagaaaaaaa attctccctt tcagagactt tcaaatattc atttgacagt
     3781 atgatcgtac gaaaaaaaga tgattcagga atccactcac ttgaagatct taaagggaaa
     3841 aaagcagctg gcgaacccaa tactagctat atgcgtttag cggaaaaaca cggagctaaa
     3901 ttagtcacat atgacaatgc cacgaatgat cagtatttaa gcgatgtagc aaatggtcga
     3961 acagatgtta ttctaaatga ttattatctg cagaaaatgg ccgtatctgc tttaccagat
     4021 atccctgtaa aaatactgga gaacgtttat ttcaatgaaa acgaaagtgg ttttttattg
     4081 aaagaaggaa atgatgctct gaaaaagaaa atcgatggcg tattaaaaga gatgaaagaa
     4141 gatgggacga caaagaaaat atctgaaaaa tattttcaaa ctgatgtttc agtcaaaccg
     4201 aaagaaacga tggctgagtc ggtgagtgag tgatgtactt tcctctaatt gatttttccc
     4261 tcatgtggac ttcacttcct ttcgtactgc aaggcttggt atacacatta gggattggct
     4321 tcgtgtcgtt tcttttagga aatctggtag ggttgctttt gacagtttta ggtcttttgg
     4381 attggctgcc tctgaatgta tttattcgtt tttatttatc gttctttaga ggcattcccg
     4441 cactagtcct gctgtttctg ttatattttg ggttacctta tcaattaagt gccttaacgg
     4501 cttcagtcat ctgctttacg attaccagca gtgcatttat tggtgaaatc tatcgaggat
     4561 cactagcagg cgtttcttca ggtcagtggg acgctggttt agcttctggt ttttctttct
     4621 ttggggtatt gaaatatatc gttttgccgc aagcttttcg aatctcagta ccggcactca
     4681 gtaacgtagc tatggatcta gtgaaaggta cttcgttagc agcaacaatc acagtgccag
     4741 aaatatttca aaaagcaaag atcataggcg gtcggacatt cgattatttt tctctctata
     4801 ttttagttgc actgatttat tgggtcattt gtatcgggat cgaaaaagtg caacagtatt
     4861 tagaaaaacg ttctatgcat cagtcaatct gaaaaataga aatggtgcta gcgtggctgg
     4921 caccatttct attttttgtt tcttgtatat tctgcctagt attttaaaac catttcatgt
     4981 aaaatgtaaa tcgaaaatat gttcggaggt agaaaacatg aagaccccgc taaccatcga
     5041 aatagaatcc tcgttatacc aatattgcat cgagcaaggc ggcttggttg tagaagaagt
     5101 cacgatgcct gatgaccaag gaattgtaga tacgcttagc tgcttgacaa aatttgatgg
     5161 aagccgagaa tggcgatgct atgagctgaa agtgacaaaa gcagattttc gctctacagc
     5221 caaactgtca tttgttggac attataatta ttttgtttta ccaagtaaac tatatgaaga
     5281 agtaaaaact gaaatcccta caggaatagg tgttttagtg tatcgtccgt acacggttga
     5341 agaggagatg cctgcctctg gtacatttct gattgcgaaa aaagcagcaa agaaagagct
     5401 tgctgtaccg gaagaagctt tgacaaaccg cttcatggct tctttattca gggaagtaag
     5461 aaaagcaaag caaatggagc gaggcgtaaa atattttcca acagatcaat tgtacaaaga
     5521 attgaaaaga cgttcagaaa atcaagatcc attttctaac gaacgatatt atgaacggtt
     5581 tttagaggaa atagctagtc aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca
     5641 gcatgattat gaatatttga ggcaacaaca gcagatccgt cggttgccta cagagccatt
     5701 ggagtgaaaa atatgtacta tccttattta agaggaaaac aatttgacct attagcatta
     5761 aaagaagcac tgtcgcgagg attgctctcc aataaaatcc aaccagtgat cgaaccagta
     5821 cgtgattccg ctacgcttaa aaatgtgatc gaattatttc aaaaaaagca ccatccaatc
     5881 gctgtgatcc aaaatcccca agtaggacaa ttcaaattat tcgatcagca tgtgcacagt
     5941 tggacagtca aagaaaatag ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc
     6001 agtgaagtac tggattcacc tccagcgttt cttgttttcg atggacagca ctacccaaaa
     6061 gatacagagg tgtggaaaca gttggcagga gttgattcga aatttttgat accagatact
     6121 agtcgctttc gcatctggct gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg
     6181 agaaagcatg ttgaaagtta tgcagataaa acagatgact tttttagtga tgactatctt
     6241 tttttccacg cagacggata tattggattt tctgatttca cgatcgaagg tagccgttat
     6301 ttcgataaag ggttccctag ccgagcgctt gctatccatc taacgtatat tgatgcttac
     6361 ggcaatatac gcgtgaaaca ttttgtctct gacagtaacg actctgctaa agaccaagca
     6421 ttgaagtttc ttgaagcagg agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa
     6481 ctcttgatta cttcaggaat gattgaatta ttggctcttt accagcaaca aaaattccct
     6541 ggactgggag tgttgaaaaa gtggtcactt atgcatcatt tggaacttat tagtcacctg
     6601 ctagatcatc caaaagattg gcttaaatat tattcaaaag ttcaggaact atatgaagtg
     6661 atccaatgat tagataatgt aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat
     6721 tcggcaaaca aggaaagaag aagtagaaga agtggtagaa atcattaatg ccgcagcgga
     6781 tttcattgct tcgcaaggct ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac
     6841 gattttgaac gatattgaaa gaaaagaaag ctatgtatta atagtggaag atcaaattgc
     6901 tggaacagct gcattagtct caagcgtgga tcccgtttac acaaaaatag atggtagttg
     6961 gaaagaatca actggtcctt atttatcgat tcatcgtgtc gctttagcag aaagtactcg
     7021 aggaaaaaaa ctagcaaaac cgttcttaga acatttgttg acgattgcag ccagcaaaaa
     7081 tatccaagat atccgtatcg atacttatcc agctaatgaa ccgatggaaa agaccatcta
     7141 ttctgctgga ttcacttatc aagggatgat tgaatttcct tttgaacacg gcgaaagaaa
     7201 agcatatcaa ataatcatca attgactata aaatcatctg agcaaagata aagacattaa
     7261 cactactgaa aaaaacaatc atatgatagc caagaggttg taacagaagt ggccagcttc
     7321 aaaaaacaag aaagaatttc cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt
     7381 ttacgaaata gctgctttct gaaactccct tcatcgagag gtcgtgacat agcttttgtc
     7441 acgacctctc ggcttatcct acgagtatta ttcgatacat ttatatcaat aatccttttt
     7501 ttattttatc tgcagcttct tggaacgctc catcgtttga cacgactaaa agtgagggga
     7561 tagctttcaa acgtttcaac tgtgctaaat cttctacata aaccgccagt ctggctgaaa
     7621 tctgttcaga tgaatctcca cgttttttca ggcgcgattg caatgtttct ttagagatcg
     7681 taataaagat aggacggact accgtcccga aatactcata taaatgccaa aaaccatttg
     7741 ctgtcaaaac ggcgtaacaa tttcctactg tcagtttctt tttaatttct gctttactgg
     7801 aaccatattt atgttgagca tattggtccc actctgcaaa ctcatttccc ttgatcatct
     7861 gattgaatgt attgtcagaa acaaaataat aatcctcttg atctttttcc ccaggtcttg
     7921 gtgatcgatt cgtgtaagta atgatctttt gttccggctg aaaaatagct tctgcgagtg
     7981 aagtttttcc agaaccagaa ggaccgataa atacatagca ataattcatt gtcatttctc
     8041 ctttgatgat tgaacaagtg aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa
     8101 ctttgctttt ttgtatatta taccagcaag ccagtcacaa tagcacttaa aaagctgact
     8161 aaagtcgctg ttagcaaaat tttcaatccg tgttttgcga caagatttcc tttttcttca
     8221 tttaatccct tcatcgcacc agaaatgatc ccgatagaag agaagttggc aaatgaaacg
     8281 aggaagacag atataattgc tgttgtacgc tcagtaaaat ggaaattacc atttgctaat
     8341 tctgtcatag caacaaattc atttgtaacc agtttagtcg ccatgatact accggcatcc
     8401 actgcttctt tccatgggat tccgctgatg aaggcaagag gggcaaagat gtaacctagg
     8461 atctcttgga aagtgatgcc gaatatcccg tggaagatag cattgatcat ggcaatcaaa
     8521 gcaacaaaac cgataagcat agcagcaacc gtgatcgcca catggaaacc atcaagaatg
     8581 tattctccca acatttggaa aaatgtctgt tctttgtttt cttcaatgat cagctcatct
     8641 tctttttctt ctaatacata gggatttaca attgaagcca gaatataacc gccaaataag
     8701 ttcaaaacaa gggcagtgat cacatattta gaatcgatca gtgccatata agatccgacg
     8761 atcgacatag aaactgtaga catagccgat acactcatgg tgaacaaacg tttctcagat
     8821 aggaagggta attccttttt tatggaaata aaaacttccg attgtcctaa aatagcagaa
     8881 gctactccat tataagattc caatttgccc atcccgttga ttttacttaa tcctagacca
     8941 acagctttca taaataatgg aagaatcttg atatagcgta gaataccgat aatagcagaa
     9001 ataaatacaa ttggcatcaa tacggaaaga aagaaaggtg cacttccttt atttgctaca
     9061 ccggcaaaaa caaagtcaac accttgccca gcaaaagcaa gaagatgatc aaacattttt
     9121 gccaatacag aaacaaccgc tgtaccaaaa gttgtacgta ataatacgaa accaagaaca
     9181 agctgtaaaa cgaacatcac aattagtggt ttcaatttga ttgctttttt gttactgctg
     9241 atcaaaaaag caagccctaa tgtgacaaat aaaccaataa tacctaataa ataactcaaa
     9301 taatcactcc tattccaaat tccgttttat tttacctgtt ttgcaaataa aatactaatc
     9361 tttagtgaaa ttttcattac ttttttagag tgaaacgcat tcattttcca ttagaaaata
     9421 aagcgaacaa aataaaaggt ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg
     9481 aaactatcga ttaagctaat agtttcgttt tcaacattta taagtaaact ttttctattg
     9541 gtaatataga ttttcggaag ggtagacagg tgcgcaggta atatcgattc ctagtttttt
     9601 caatgtttgt tcatcatttt tattgaccat cacagtagag tgggcttgtg ctccctcaag
     9661 ttcagctagt ttatcataag caacttgagc gattggattc gttactgcac tgattgataa
     9721 agcgatcaag acttcattga tgcttagtgc atccaaagag gaatgaagtg tctttgattt
     9781 caatccttgt attgtttcaa gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg
     9841 ggctaaatgt tttactgcat ttaagataac tgccgctgac gcatccatca atgaacttgt
     9901 acgcccggta atgatgactt catctggtaa ttcgattgca atcacagcag aaggatcttt
     9961 tgtaggactt tgcgcttgaa tcttatctgc atagtcatgg gcagggacta ccacgtttcg
    10021 gtcttcagga cgtaaaccga cttcttccat aatcagtttg attcgattaa ctgcttcttc
    10081 gtcgatcaat ccttttttga agtcgcattc tgtttggaaa taacgacgga tgatttcttg
    10141 tttagaggct tcttggacaa cggtatcatc cacgatccca aatcctacac gattcacacc
    10201 catgtcagta ggagattgat aaacagattc tttaccagta attttttcaa taatacgttt
    10261 gattacagga aatgtctcaa catcccgatt atagttcaca gctactttat tgtaggcatc
    10321 aaagtggaag gaatcgatca tattgacatc ttttagatcg actgtggctg cttcataagc
    10381 aatgttcagc ggatgtttca acggaacatt ccatactggg aaagtttcga acttggagta
    10441 accagctgca tgtccttttt ggctttcatg atacagttgg ttcaaggaag tagcgagctt
    10501 tccacttcct gggccaggag ctgtcactac aacgattggt ttagttgttt caatatactc
    10561 attttttcca aaaccatctt ctgagacgat tttttctacg ttgactggat agtcttcgat
    10621 ttctgagtgt ttatagacct tgatgtttcg tctttctagt ttattgataa agactttcgt
    10681 ggcaggctgt ccactatagc gagtgataac gacactgttg attaataagc cataaccacg
    10741 taattcatcg attaaccgca agatatccat atcataagta atcccataat caccacggat
    10801 cttattacgt tcaatatctc cagcatagac gcaaatcagt atttcagctt gatctttcaa
    10861 tttttgcagc aatttgattt tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg
    10921 cttgtcgtca actagcttcc caccaaattc taaatataat ttgtcatagt ggttcactcg
    10981 ttccaaaata tacgctgact gctcttcaat atatttttct gaatcaaaac caattttttt
    11041 cactaggttc cctccttaag tactaaagca ttttttcatt tttttgagga ttcgtcaact
    11101 ggaagagcga aattcctcaa aaaaacaaag aaaatggttt aaaccaactt gtctagatag
    11161 ttattcattg gtatgatagt ttttgataag gaaggtgcta catgaaaaaa agacagaaaa
    11221 agaaaaacgc atataaacac tatatccgtt cgatttttac cggttatgaa agaatgcttg
    11281 aagatcccga attggaacaa ttgacgttta cttatctaaa cgaagaaacg cagctaactc
    11341 gtgatgagca tcagcgcatt catttcacaa caagagatct gcctagtaaa taaaaaataa
    11401 gataaaggtc atcggattcg attctgtcct atcagatgga aagactagtt gatcgaaaaa
    11461 aaggctgtac caaattttgg tgcagcctgt tttatggagc attgcccgct cttttttata
    11521 acaatttttt acagaaaatg gtactattga agtattaata agtgaaaaac agatgagtaa
    11581 gaggaggcaa gtagtatgaa agagccagat acaaatgcaa attatgagcc agacgaacta
    11641 gaggagacga atgcggatca ggatacagga caagaatcgt cttcttttta cgatatcaaa
    11701 gaaaaagcaa gcatgaatct ttcccaatta ttagaagact tacaggcttt tgaaaaagct
    11761 gtccgcgaag aagatatccc tgagatctac cgtttataca atggccgatt gaatgaagaa
    11821 ttaaaaagaa gctcaaatga aaatcacgag atcgatcaat tgctgatgcg taagatccat
    11881 gatcgttttt tacaggaatt tccttttatg gaacaagtag aaaacatctc gccaacgatg
    11941 agctattaca aaatcggcac ttattaccat gatcggccca cggtcggtat tgatgcaagt
    12001 ctgccagaaa tttttgtttt gcctaagatc gatgaagagt gggagaaata ttcccaaccg
    12061 gagtcaacgg actatgacaa aaaaatcaat gagctagacg caaaggtgat cactgctcag
    12121 acagaaattg aacgtttaga tgaacaaata aaagaaatca atcgacaaat gactgatttg
    12181 aacgacaata aaggattctt gaatcgtaaa aaaatcgagg aagaaattca agaattagag
    12241 aagaagaagc aagtactatc taatgaaaaa ttaggctggc ttccttatat tgagacgcct
    12301 gaaacaatcc agcagcaaaa agaagcattg aagcaagaag caagagcaga ccagcttcgt
    12361 gcagccatcg tagaaaaaga acaaaggcaa atcagacgct atttcggtag caaagaggga
    12421 ttcggtcaag caatccatga attcttgatg aattatcttg gcaatgagaa tcctaataat
    12481 caatctaatg aaggaggttc cgagtatgaa tgaacacgat gaagcagaat ggacaaaaaa
    12541 tgaagaacaa acagaagatc atgaagaaac gaacacttca ttggaagaac aaacaagtaa
    12601 tcctctggat gacacagaag aaatccctat gctagaggaa acaaaaatac acgaaaaaga
    12661 aaaagtaact ccgacgaata cgaatactgc agaaaacacc catgcttacg aaataactga
    12721 aaatcccggg tatttagaag gaaaagagca agatcatcaa gatatcgtca gtttgattga
    12781 aacagcggaa gcagaattag cggatttacg tatgagaaaa gatttgatcg aatcagattt
    12841 tccacaatta ttagaatcat atgatcgttt tatattgaat gaagaaccaa tctccacgat
    12901 cggaaagaca agcctgatcg aatacttcac gtatgaatta ctgaaaccgt tgaatgatat
    12961 tggtctggaa cggtcaaatt cagcttatga cacatggaaa acaagacatt tttcgatcaa
    13021 ttatacgttg aatggacaaa acgaattagt cttttccttt gtattaccga ccatcgatca
    13081 acgataccaa gtggaatcga tgagtctcct aaacgtatca ccagagacaa tggaaatcaa
    13141 tgtccaagat gaccgggtgc tttctttgat ccgacactgg tcagtggatc gagtattctc
    13201 tgcagggcag atcacgatat tcaatcacaa attgaatcaa ttattagccc atgcaagaac
    13261 acttggtttt accgttaatc agacattact agacaatacg aagccacttc gtttgaatct
    13321 tcagagcgaa tttgaattga cgaatgaagt attggatgat atttttatcg tggcaatgaa
    13381 aaatccatcc tacgatatgg aaaagatcaa cgaaactact tatcaagtac gattagataa
    13441 aggacagtct ttaacgataa caacaaacga aaaagatcaa acagtcttgt cgatctcatc
    13501 tggagactac ccacgatctg tcattgattt ctttatcaat tacgaattct tggtcccatt
    13561 aatggtgaga aagtcctgat tggataagtt ggattaccag atgtcattca gataattctg
    13621 ttccaaagga cttgcggcag ttatccagaa taattgtcac aagtcttttc tgtatgtact
    13681 aaatggacgt gaataacttg ctgatgaagg agggatttca ttaggaatgt ccacaaataa
    13741 aatagcacag cttagcttat tgtctgctgc ttgtgtagca ggtaggatcg cttttcaatt
    13801 tattccgaat ttccagccaa tgactgctat ttttttattt atagtacttt acttaagctt
    13861 aaaagatgcg ttaattgtca tgagtctttc tattgcgatc agcagttttt attttggtgt
    13921 tggaccatgg gtcatcggcc aatggatcag ctatactgtt gttctgagtt tgttttcaat
    13981 cgtctgcttg agaaaaacag tgcaaaggaa tctatggtta aagggagtct gtttttttct
    14041 ggcgggcatg gtttacggaa taggtatgac cgtttttgat acgctgttat atcgtttgcc
    14101 tcaaccttgg atctattacc ttcaaggggt gtcgtttgat ttgatgcaca gtataggcaa
    14161 tgttgtgttc tttcttgttt ttctgccagt cgtcaaacgt ttttacaatc attcaggagg
    14221 aaaaaatgaa aaaaataatt tatagtttgc ttgcaatgag tgtcagtata ctcattttga
    14281 caagttgcag tacaacctat acacaatcgg cttccaacca aaaggaggaa gtaaaaacat
    14341 cgcaaaaagc aacaatcacg ttacaagaaa atggtcaaaa cttttcggaa aaagaagtgt
    14401 tcttcaaaaa aggcgatgat ttactgagta ttttgaaacg aaattttgaa gtcaaagaag
    14461 ataacggctt catcacttcg cttgaagggc actcacagga tgagaaaaat caactctatt
    14521 ggatgtttac tgtggacggg aaaagtgcta ctactggtgc caaagagatc aaattgaatg
    14581 atggacaatc ggtcattttc aatttgtcta aactataagt ttcataaaaa actgttaaag
    14641 ggcgaatatt gccataattc agtgagttca gttgatgaaa gaaggaaaat aacatggaaa
    14701 attttaaaaa aagcggaatg ttttccgtaa ttgctgctct aggggctaat attctagttg
    14761 ctatcagcaa atttgtcggc tatgcaatca gtggcagtgc cgccatgcta aacgaaagca
    14821 tccacagtat cgttgattgc agcaatcaga ttttgctgtt attcggtgat aaacaagcca
    14881 ataaaggaca gagcgagttg catcaatttg gtgaaggacg agcaaaatac tttttcagta
    14941 caatcgtcgc aatgatgctt ttcttcggcg gtggggcact tggggtgatg gaagcttttg
    15001 aaaaattgac gcatccatcc catgaagtgg gtaatccgat cattgttata gcaatactgt
    15061 tatttggttt ggtaatcgaa ggaagttcgt tacgcgtggc aatgaaagaa atcaaagaat
    15121 tgaatactga aaaactttcc acgatgcgct ttttgcgcga aagtcgacac agtgaaatac
    15181 tgattatttt tacggaagac ttgtgtgctg taatcggttt ggtcttggct ttagcaggaa
    15241 caatattgac ttatgcaacc gggattgctg catttgatgc gatcagcgga ttgctgatcg
    15301 gtttcttgct gatgggagcc gccattttcc tagcaaaaga attttatagt ctgatcatcg
    15361 gtgaaagcgt gacagcaagc gatttgtcaa aaatcaagtt agcctttgaa cgacctgaaa
    15421 tcagtcggct aattgatatc aaaacagttc atttaagtcc aacggagatt ttagttgcag
    15481 caaaagcaga tatcgttggt aacaaagaga atgaagctta ctcggtcatc aacgatattg
    15541 aaaaaaatat gcgtcagtct cttcctgaca agaagatgta tatatacatt gaaacagata
    15601 aatatgaccc aaattattct cgtaaataaa acgtttattc atgaattgaa gcaaaaatga
    15661 gaaaaatata atcttgtttt taaataaaac ggctacaatt tagcgaattt atgttacaat
    15721 gagtccgctg tttatttgtt gtaattaaag gagatatttt aatgaacaaa aagaaaagtg
    15781 cgccatggct tcaagctttg catgcagtga tgccactttg tataagctat ataccagtag
    15841 gattagcttg cggagtattg ttgcaaaaag ttggatttga tcctctctta tcaggactgt
    15901 tatcgattct ggtcttttcc ggtggcgcgc agtttttagt tgcatctatg cttacgacgc
    15961 aagcatcatt tgcaacgaca ttattgatgg ttttcttttt agaattacgc tatacattat
    16021 taggagcaag tcttgccggt tttatgaaaa aagaaaagcg gacgtttttg gctgtctttt
    16081 ctcaatcact gaatgatgaa aactatgcgg tgaactactt aaagttttca actgaccctt
    16141 cctggaataa aagaaaggct ttatatgtca attggttctc aatgactgca tgggccggta
    16201 gcaatatgat cggtaatttt ttcggctcgg tgattcgagt agatgctgac cttgtccatt
    16261 ttgctttgac ggccatgttc attttcatgt ttatcatgca gatgaaaagc gcactactga
    16321 tctttacagg tttattctca ggcgtattag gggtcgtatt catggtatta ttccaaaata
    16381 ctttcggatt gattgcagca acagtcattg cttcatttgc cggatttatg ctggagaagg
    16441 cattcaaaaa agaaaaagct aagaaactaa gagaccgcgg taaagatgtc aatgagccgc
    16501 tcttcaactt cccggaacag gaggccaatc accatgacta aattatattt gctactttta
    16561 gttgcttgtc tgttcatcgt ggcatatatc ccgcgcttgt tcccgatgct ttattttact
    16621 catcgaaaag tgccatcctg gttcagtgac tggatgaaat atgtaccggt ggcattattt
    16681 gcggcgttag cttttaaaga tgttttcatt actcatgaac atctggatat tgcttggaat
    16741 atcaaaatag ctgcaatgat tctagtagca ggcgtggctt acaaaacacg ttcgatggcg
    16801 ttgtcagttt tgacaggact tgcgtctgtc tttttgctgt ctatgcttta aactgtttta
    16861 ttttattaaa aacagagatt gcgacataag ttgttcaaac tgatctatcc gaactgcatt
    16921 tgcaacagtc aattctggat gcaagtttat tcgtagggat aaaaaagatt agtgttggct
    16981 tatgatgcaa gctctttttt attttaggaa ttagagaggg atttgtcctt ctatgatttt
    17041 ttgctataat agggaagatg tttattttat gaaagaatag ttgagggaat ttaaatgaat
    17101 aaaaaattga tgcaaggaac attttggcta acctttgcta atcttctatg taaagtttta
    17161 ggggtagtct atttgattcc ctggctaagt atgatgggga acaatcaaga tggtatgctt
    17221 gctacgacat tgtataatgt tggctatctt ccttatggat tgttcctgat gcttggtaca
    17281 gtcggatttc caaatgcgat tgcaaaaaaa gtagctgtag ccacaaaaga aggagataac
    17341 gctgcttgtc ggctgatttt tagaagtacg atcaatatca tgtttgtcat cggtatcgcc
    17401 tctgctgcac tgatgtattt atttgctcct ttactagccg gaatcagtcc gattgctaac
    17461 gttaataatg ggatactagc tatcagaagt ttatgcccat ctttgattgc tatcccaatt
    17521 cttagtgcaa tgagaggata tttccaagga aaaaatgatc tgcggcctta tggaacttct
    17581 ttgattatcg aacaggctgt gcgagtgatc gttatcttgg ctggtactta ttacttgcgc
    17641 gtattgactg acggaaccat attagaagct gtattgatca gtacagtcgc ttctttcttc
    17701 ggtgggttag ctgcgattat ccatatgtat atcgtcggac gcagaaaaga cttcttcgaa
    17761 cttaaagatt ttttgatttc cagccgttat ttcgaaagag aaaaccgatc tgcctctgtc
    17821 tctatcattc gggaaacact accgtttatt tttgtcggct cagtcattac tatcgttcaa
    17881 atgatcgacc aagtaacgat gaaaccactg cttcactttt tccgtccaga gatcgcagat
    17941 cagcaactgg aattcttatt cagccgtgct tctgtcaatc caaataaatt gacattgatc
    18001 ctgatctcca tggtaggaac agtagctata agcagcttgc caatattaag cacgatgaag
    18061 aaaaaagatc ggatacaaat cgaaaaaaca gtaggagata gtttttcgat tgcgttattg
    18121 attcttcttc cttcattgac tggaatgtct ttattagctg gaccactata cacattgttt
    18181 tttggctatg atcctgaaag tgtcggatat ttccaaatgg ctttgctagc ttctttgttt
    18241 ttctctttat tcacgatcca atctacgatg cttcaatctt tgaatcacca tcgttttgcg
    18301 atccgattaa ctgtagaagc aattatcctg aaagtcatct tcgaagtaat cggattagga
    18361 ttagttggag gatacgggat gagcttatca aatggcttag cattcggtat tgtctttgtt
    18421 cgcggctatc actttatgtg caaagaatac cgaatagcac ctttagcaaa gatcagcaat
    18481 tttttcttaa aaactttccg ctccaccctg attatgcttg cagtatgttt tgccgttttc
    18541 tttttgctga atcagcgatt gtcaatggaa tcaaaaagct atgcaatgat ttattgtgtg
    18601 gctgtaggaa gtattggcgg cttagtattt ttattttctc aattcggaaa aaatgggttt
    18661 cgaatgttga aaaatgttaa acatcatagc agaagcaaag aataggttaa ctaatcgttt
    18721 tttgctgtca cttgaaaaac ggcaactcgt atgattacgc ttgtcgtttt tctattttcc
    18781 aattttagtt tcatatctaa tcttacttat gatatagtta taaggtctgg aaaaattctt
    18841 tgaaacgagg aaattaaaca catgaaactt gcagtagtta cagatagttc ggcatatctg
    18901 ccggaacgta tcaagaacca tcccgactta ttcgttattc caatcccttt gattatggat
    18961 gggaaaattt ataacgaagg aatcgatatc gaagcggatg aatattatgg acttttaaaa
    19021 aacagtaaag attttcctac tacttcacaa cctgtcgtag gggaagtttt gggtttatat
    19081 gaggaattga aagagaaagg gtatgacacg attttaagta tccatctttc ttcagggatt
    19141 tctggctttg tcaacacatt atttgcgatg aaagatgaca tcagcggagt aacggtcatt
    19201 ccttatgact ctaagatcac tagtatgccg atgggacaca tggtagaagc tgccttagac
    19261 ttaaatgatg aaggtcagtc ccttgatgaa atacttgcac atgtcgatcg aatccgagat
    19321 aatacctacg cttatctgat tgtagatgat ttgaacaatt tagtacgagg aggacgtttg
    19381 acaaatggtg cagcgctgat tggcagctta ttaaaaatca aaccgatcct gacattttct
    19441 gaagggaaaa ttgttttatt cgaaaaaatc cgttcaagta aaaaagcatt tgctcgagct
    19501 gaagaaatta ttggtagaag aaataacgaa atacaaccgc cagtcaaact ttacgttata
    19561 catgcaaata atttagaagt agcgattgcg gagaaagaga aattgcagaa aaaatatcca
    19621 aatgcagaaa tcgaaattgg tcatttcgga ccagtcattg gtacacattt aggtgaaaaa
    19681 gccatcggca ttgccatttc agcgcaataa ttccttttat tcaaaaaaca taccaaaaat
    19741 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
    19801 gtgtttttat ttctatcaac gaagctcttt ttctttgcaa tccacacttc aatcctctat
    19861 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
    19921 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
    19981 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatacggac gcaaaagggc
    20041 tttctctggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
    20101 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
    20161 tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct tatcgctatg
    20221 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattgcaa gggaaccgaa
    20281 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
    20341 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
    20401 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
    20461 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
    20521 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa ttaaagggaa
    20581 ttggcaagat cctgaaatga aagtcagtaa cgtacttttg gaaggagatg ccgggtcggg
    20641 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
    20701 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
    20761 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
    20821 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
    20881 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
    20941 tccttctgaa atcgttcgag cgattgaaaa aggatattta ttagagattc aagagccgac
    21001 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgcttgaac caaatgggat
    21061 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
    21121 aaatcgaaat taccaaggaa atcgtccatt aaatgaatct ttaagagatc ggatgcaaca
    21181 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
    21241 catgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
    21301 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
    21361 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
    21421 attatccact gatccagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
    21481 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
    21541 tacaaaagga agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
    21601 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
    21661 agaagagaaa caagaagaaa caatagaaaa aaacgagttt cctcctcaaa tgcaaaaaga
    21721 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
    21781 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
    21841 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
    21901 accatctttc caaggaaaaa cgccggaagt acgtgagcta cacatgcaag taatgccaat
    21961 agtagaatca cttagccgac aaattttaga gttactggat aatgaac
//
LOCUS       DAGCYL010000026        21904 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.026, whole genome shotgun sequence.
ACCESSION   DAGCYL010000026 DAGCYL010000000
VERSION     DAGCYL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.026"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1416
                     /locus_tag="MB216_000793"
     CDS             <1..1416
                     /locus_tag="MB216_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4525651.1"
                     /translation="NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAM
                     VIAAALVYPTIVSAYSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSL
                     HESIRNLLTPFICIIVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGF
                     WQVLVIFGLHWGLVPVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKS
                     LSLSAFITALFGITEPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSI
                     LSFPIYIGEGFVWLVASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIID
                     LSEVNDPTFASGSLGEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIH
                     IGIDTVNLNGKYFQSAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKN
                     IITSHKKNNQTRKLASLGGSI"
     gene            1413..2870
                     /locus_tag="MB216_000794"
     CDS             1413..2870
                     /locus_tag="MB216_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4525652.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            3454..3888
                     /locus_tag="MB216_000795"
     CDS             3454..3888
                     /locus_tag="MB216_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4525653.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            3989..5080
                     /locus_tag="MB216_000796"
     CDS             3989..5080
                     /locus_tag="MB216_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBT4525654.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            5110..5787
                     /locus_tag="MB216_000797"
     CDS             5110..5787
                     /locus_tag="MB216_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBT4525655.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            6119..7414
                     /locus_tag="MB216_000798"
     CDS             6119..7414
                     /locus_tag="MB216_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBT4525656.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            7404..8864
                     /locus_tag="MB216_000799"
     CDS             7404..8864
                     /locus_tag="MB216_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4525657.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            9000..10496
                     /gene="glpK"
                     /locus_tag="MB216_000800"
     CDS             9000..10496
                     /gene="glpK"
                     /locus_tag="MB216_000800"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBT4525658.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            10510..12333
                     /gene="glpO"
                     /locus_tag="MB216_000801"
     CDS             10510..12333
                     /gene="glpO"
                     /locus_tag="MB216_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBT4525659.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            12334..13050
                     /locus_tag="MB216_000802"
     CDS             12334..13050
                     /locus_tag="MB216_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBT4525660.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            13356..15284
                     /locus_tag="MB216_000803"
     CDS             13356..15284
                     /locus_tag="MB216_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBT4525661.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            15410..15964
                     /locus_tag="MB216_000804"
     CDS             15410..15964
                     /locus_tag="MB216_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBT4525662.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16449..16763)
                     /locus_tag="MB216_000805"
     CDS             complement(16449..16763)
                     /locus_tag="MB216_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBT4525663.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(16777..17037)
                     /locus_tag="MB216_000806"
     CDS             complement(16777..17037)
                     /locus_tag="MB216_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBT4525664.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            17207..17521
                     /locus_tag="MB216_000807"
     CDS             17207..17521
                     /locus_tag="MB216_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBT4525665.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            17523..19178
                     /locus_tag="MB216_000808"
     CDS             17523..19178
                     /locus_tag="MB216_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBT4525666.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            19261..21396
                     /locus_tag="MB216_000809"
     CDS             19261..21396
                     /locus_tag="MB216_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4525667.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            21571..>21904
                     /locus_tag="MB216_000810"
     CDS             21571..>21904
                     /locus_tag="MB216_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525668.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFE"
BASE COUNT         7292 a         3689 c         4772 g         6151 t
ORIGIN      
        1 aatttattaa ctactgaatc aggaacttat atcattttga atgcagcagc tgatgcattt
       61 ttctatttct tgcctatatt tttggcatat actgctgcaa aaaagttcaa tacagatcgt
      121 ttcatcgcta tggttattgc agcagctctc gtttatccta cgattgtcag tgcatatagt
      181 gatagcataa ccttgcgatt tctagggatg cccgtgattc tagcaagata tacaagtacg
      241 gttattccag caattttagc tgtatgggtt ctttcttata ttgagcctaa gattcgcaaa
      301 agcttgcacg aaagtattcg aaatttattg acaccattca tttgtatcat cgtgatggtc
      361 ccaattactt tattagttgt aggtccaatt gcagattatg caagtcagct gattgcagct
      421 ggatatttag ctgtttataa ctttagccct gttttatctg gtgctgttat cggcggattt
      481 tggcaagtgt tagtgatttt tgggttacat tggggacttg taccagtaat gaccaacaat
      541 ctttctttct acggtcgtga tacgctaggt ccagcatgta tgactgccgt agctgcgcaa
      601 gcaggagcag tacttggggt atttttgaaa acgaaaaata agaaagtaaa aagtttatct
      661 ttatctgcat ttatcacagc attatttgga atcactgaac ctgctgttta tggtgtgact
      721 cttaaatata aacgaccatt ttacattgct tgtatatgtg gagctatatt tggtggagta
      781 gctggtgccg caggagccgg tgctttggca gttgctacaa gaagtatttt gtctttcccg
      841 atttatattg gtgaaggatt tgtttggcta gttgctagtt atttcttagc gatgatcagt
      901 tcctgtatgc taacttttct tttcggctac aaggatgaaa tcgaagaaga atcctcaaag
      961 gatatagtgt taagtactcc tgcagcaggt gaaataattg atttatctga agtaaacgat
     1021 ccaacatttg ctagtggatc tttaggagaa gggtttgcga tcattccaac ggacggaaaa
     1081 atctacagtc ctgtaaacgg ggaagtttca acagtttttc caacgaaaca tgcaattggg
     1141 gtagtcagtg aagaaggagc agagatactg atccatatcg gtattgatac agtgaattta
     1201 aatggcaaat actttcaaag tgcagtatct gatggaaaaa aagtacgtaa aggcgattta
     1261 ttgatggaag tagatttgca agaattaatc aaagagggat atgatccgac cacaatggtt
     1321 attgttacaa atagtagtag attcaaaaat atcattacta gtcataagaa aaataatcaa
     1381 acaagaaaac tagcaagttt agggggatcg atatgaaaaa atttccagaa ggatttttat
     1441 ggggcggagc gacagcggcc aatcaatgtg aaggtgcatg ggatgtagat gaaaaaggat
     1501 tatctgtgtc tgatgtgtat acattcgaca gcgatttacc taaagaaaaa tggacagatc
     1561 aatggcatat gatgacgcat cagcaagtaa aagcggcgca ggatcccaac agcaagtatt
     1621 attatcctaa aagacacgga aatgatttct atcatcactt taaagaagac atccgtttat
     1681 ttgctgaaat gggttttaaa tgctatcgga tgtctatcgc atggacaaga atttttcctc
     1741 atggtgatga agaaacaccc aatgaagcgg gattattttt ttatgatcaa gtgtttgacg
     1801 aatgtctaaa atatgggatt gagccagtcg tttctctttc ccattatgaa atgccgttat
     1861 atttggttac agaatatggt ggatggccta atcgaaagct tattcagttt tacgttcgtt
     1921 tcgctgaaac agtattcaaa cgctataaaa ataaagtaaa atattggatg actttcaatg
     1981 agatcaactg tgtaaaacat cacccttttg ttagcgtagg agtaattgaa gaaaaccatc
     2041 cccatttgga acaagcgaaa taccaaggtg cacaccatca gtttatagca agtgctttag
     2101 ctacaaaagc ttgtcatgaa attatcaaag gttcacaagt cggttgtatg atcagctatc
     2161 agcttcttgt tccgtattct tgtgatccgg atgatattca aaaaacaatt gagcagcaaa
     2221 gaacttcttt gttctttagt gatgttcaag caagaggata ttatccagcg tatacgcaac
     2281 gaatgtttga agaaaaaggt gtcaacttaa aaatagaagt aggtgatgaa gaaatcttag
     2341 cagcctaccc tgttgatttt gtttctttta gctactacat gtctagtgca gtcagtgctc
     2401 atccagaaaa tttggaaggg gtccaaggaa acttaataac tggaggaatt aaaaatccgt
     2461 atcttccatc aagtgaatgg ggctggcaga ttgatcctaa gggtttgaga attgctttga
     2521 atcaattata tgataggtat caaaaaccac tgttcatagc tgaaaacgga ctcggagcta
     2581 tagacaaagt agaagaagat ggaaccgttc aagacgatta ccgcattgac tatttgaagc
     2641 agcatattga gcagatgaag gaagctatcg aagatggagt agatttattt ggttatacgt
     2701 cgtggggctg cattgatatc gtcagcgctt ctacttcaca aatgtcaaaa cgttatggat
     2761 atatctatgt tgatcaagat gacgaaggga atggcacaaa gaaacggtta aagaaaaaat
     2821 catttgattg gtacagacaa gtcatccaat caaatggcga aatcctataa taaaaggaaa
     2881 taagggactg tgacaaaagt cttgtcacag ccccttcttt acgaataaac agtattctcc
     2941 tgccaactcc tcgatcacaa gtcaccatag tcataaaaca tgagaagctg ttttttgaaa
     3001 ttgctccttg tgactcggag ttcaacggca ggctcacgac ctctcaataa acggtgtaca
     3061 atcagctgac catcggcatt agctcaaaaa acgacaaaat atgaggaact attttggttt
     3121 ggctgttctt attacttgat gcaggacggc tttttcacaa cctctgatat aaacggtgtt
     3181 caaaagccag tttttcggta ttaagccaaa tttatgcaaa aatgtggaaa gccattttca
     3241 caaaattttt cttcatactc aaaactaaac gactttttca caacctctga ttcacggtgt
     3301 tccagaagga gttttgtcga aaacaagccg aaattctttg aagatggcta cttctttttt
     3361 tcaaatctag acagaggagg cggacattat tccgtgtttt ttgacaaaaa ataaaaatca
     3421 tactatgata tgtacgccaa tggaggggat gaaatggaaa agccaattga aaaagcgtta
     3481 gatgaatgtc ttttctttag cgtgaaaaag ctagatcgca tgttgaataa attagctgat
     3541 gaagcgttta gaaggacagg tctggcacca acttatggtt ttattttatt gatattaaaa
     3601 gaaaaagatg gaataccaca aaaagatatt gcacaaatgc tctattccgc cccatcaacg
     3661 attgctcgtt ttgttgaaaa attagaatat aaaggatacg tcaaaacagt tagcgaaggt
     3721 cgcttgtcat tagtttatct tacagaacaa ggacgccaat ttgctaagga agtcgatggt
     3781 agctgggaag aacttcatca gtcatacaaa gcagttttgg gaaatgaagc aagtaaagaa
     3841 ttagcccaga caatgaatga tgcaacagat aagttacaaa aaaaataaaa ttttttaagc
     3901 aaattagttg catgtacaac taatcgagtg gtttaatgat agatgattct cgataggtta
     3961 atcagagcga attatgggag gaatgacaat gtcacaattt gatcaatcaa ttactttacc
     4021 ttcaggagtt caattgaaga accgattgat gctgtcgcct atgacgacac aactcagctt
     4081 tttcaatgga gtgatcaccg aagatgagat cacttattat aaacaacgtt ctaaaggtct
     4141 tggtgctgtt attactggtg ctgcaaatgt ccaagatatc ggaaaaggct ggccaggtga
     4201 actcagcatc gctcatgatg aaatgattcc tcgattaagt gagctagcga aagcaatcca
     4261 aggagaaggc gcaaaagcga ttattcagat tttccacggt ggacgaatga caagtcgagc
     4321 aactttatct ggagaacagc ctgtatctgc aagtgcagtt gcagcaaaac gtcctgatgc
     4381 ggaaacacca cgagcaatga gtgaagaaga aatcgttgaa acaatccaag catttggcaa
     4441 agccacacgt agagcgattg aatcaggatt tgatggaatc gagttgcatg gagcaaatac
     4501 gtatttgatc caacaatttt tctctccgca ttcgaataga cgagaggatc aatggggcgg
     4561 tagcttggaa aatcgttatc gttttattga agaattatta acaacggttt tctcagcggt
     4621 tgaacaatat gctaaaaaac cattcatcgt agggtatcgt ttctcaccag aagaatatga
     4681 aacaccaggt atccgttttg aagacacgat ctggcttctt gaacgattac gtgagtcaaa
     4741 actagattat ctgcatgttt cattaaatat gtatgatcga atagctcgtt ctgataaata
     4801 caacgataaa tcgattcttg aagtggtcca taacacgatt caaggaaaga tccctttagt
     4861 tggtgtcggt gggatccgca atcgcgaaga tgtagagcag gtattgaaat atgcagaact
     4921 tgcagcaatt ggacagcaaa tgatcgtcga tcctgactgg gcagagaaat tattggaaaa
     4981 ccgtgaggat gaatttgtaa cgaaaccttt tgaagaagca tataaagagc tttatttgcc
     5041 aagcccatta tataatttct tgaaagcacg ttatcaataa cgcaattttt atagaaagag
     5101 gatgggaaaa tgaaagtatt gatcgtatta acaaatgtag aaaagtacga aaagatcgag
     5161 cgtcctacag gtttgtggtt aagtgaactg actcattttt atgatgtatt agtaaaaaat
     5221 gggatcgatg cagattttgt cagtcctaaa ggcggatatg tacctttaga accgcaaagt
     5281 gttttgaata tggatgaaat cgattgggcc tattatgaag atgaagattt cagaaatcgg
     5341 gccttgggaa ataccttacg tcctgatgat gtggatgcaa atgattatgc agcggtttat
     5401 tatgctggtg gccacggaac aatgtgggat tttccagaag caatagatct tgggaaagtc
     5461 gcttcacaaa tctatcgaaa tgaagggctg gtctcagctg tttgtcacgg tgttgtagga
     5521 ttgttgccag ttacagatga gtctggaaaa tctattttag ctggacgtac agtgactggg
     5581 ttcagtaatg aagaagagga agcaaatggt acaacgaatg aagtaccatt cttagcagaa
     5641 gatgctttga aagaaaaagg agcaaactat cagtctggag cagctttttc agaagtggtt
     5701 caaattgatg ggcggcttct tacagggcaa aatcctcaat ctgctcatca attaggcgaa
     5761 gcagttgttg aagcattaaa gaattaaaaa taaatgatgt gtctattcta actattacat
     5821 tacacacgct aaaactcgtc ttttcgttct tttctgtttc ctatttggac agaggtacgt
     5881 aaaaggcgag tttttttctt ttataagagt acattttttg aaacatctct tcagaaatga
     5941 tactcttcaa ttaggtccac tagtacatta ttgagaagca gacctagaac acggtatgat
     6001 ggagagggtt gattctgctg catcgctatg tatcataagg ttgaaagcga gttatgaata
     6061 ctgtgttttc tgattgagaa gttagcagta gaacacctta taagaaagag gaatgcagat
     6121 gaacattgta attgtcggcg ggtcgtttgc agggattcat gcggctattt atcttcgcca
     6181 attgatgcca acaagtgaaa tatatttgat tgaaaagcaa tcgaagttag gatggattcc
     6241 cagcgggttc aatttgatat taaaaggaaa ggttaccagt gaaaaacaac tttcttggat
     6301 cacgaaagaa gaactgactg accgctatcg aatccatgtt tacacagaaa agacagtaat
     6361 aggattgaaa gaaaaaaaag tcgttttagc aggaggaaaa aaattggatt ttgatcgttt
     6421 gattctagcg actggttcaa atcaaaagtt tcgaaatatt tctgcagaat caacgcttat
     6481 tcacccagtt aaaaagatac aaaacgtaac ggctcttcag caaaagatca tgaatgcaaa
     6541 gaaaattgca attattggtg caggacaagc aggaatcgaa atagctgaag ggttggcatc
     6601 aagaaaaaaa caaatccatc tttacgaaag ccgaaagtca attctttttc gttatctaga
     6661 tcctgaaatg acagaaccgt tagtaaagga aatgaaaaat caagggcttt cacttttttt
     6721 agaagaacaa gttgtgtctt tgaccgaaaa tgaaaccgct attgtagaga cggaaaaaag
     6781 aaaggagaac tatgatcttg ttttgctggc taatcattct cgccccgaca atcagatgtg
     6841 ggaagaacaa ttaacattga atgatgatgg aacgatctgg gtagatgact acttgcaaac
     6901 gtctcaaaaa gacgtttatg cgattggcga tgcgatccaa gtaacttttc gtccaacaaa
     6961 tgaaaaaatg tatgtttctc tagtaaataa cgctgtccgt acagcacgaa atgtcagcaa
     7021 gacaattagt ggctcacaga caaaggatca gggaacctat cgtccagtcg gcaatcagtg
     7081 gtttggctat ttcttaggaa gcgtaggttt gacagaagaa gagagcattt tttatcccca
     7141 aaaaattgaa acaggatacg tagactacaa gcttacagca ataaatcaaa gcaaagttaa
     7201 actcaaattt ctatttgatt ctgaaaaaaa gattcttgga gcacagctcc aaagcaaaaa
     7261 agagatgttc caccttcttg accaactgac aattgcagta aaagaaggat ggacgtttga
     7321 acagttagaa gaacatgagc tatttttcca gccagaatac cgtgtgccag atatggtgtg
     7381 gtcaaaggtg gcgaatactg cctatgaaga ttgaagcatt attagaaaga aaagagcagt
     7441 tacagatcct gattttgcga aaccttgttc tgcaaggggg aacagcctct atcaatggtt
     7501 tgagagagca tgtccaactg tcaaaagcct cgtttgatca atatttggaa gatattgaat
     7561 tgattggccg aatgatggaa aaaaaagtag aagtacagcg aaatgagtat caggcgctgc
     7621 ttgtgttgga cgaagatgta agtttggaaa aaattctatt gtttttattg caggaaagct
     7681 tgaagtttaa gatgctcgtc tatttattag aacatcagca agtatccatc gttcgattag
     7741 cgacagcgtt taatatcagt gagtcttctg tttttcgaaa gatcaaagaa ttgaaccagc
     7801 tcttagagga atttggatta caaatcaaaa acggtcaatt atacggagaa gagctacaga
     7861 tacgatattt ttattatgaa ctattccaat atatcccgga agatcagcga ccgctttttt
     7921 tgcaaaatac accagaaaaa agaccgttca ttctaggatt ggaccgtgta ttagaaacaa
     7981 cgtttacggc atcagcagaa gcacagattg cttgctggct cgggattacg aaaaaaagac
     8041 tattgaatga aaaaagtacg tacgcaactt tgaaagaaaa gaaattactc tatcagtcgg
     8101 accggcttta tcaagcaatc gatccaatca tcgtgatgca tcttagtcga acagcagcag
     8161 aactgaatgt atacgaaaca atgatgtttt acagcttttt tgtttctttt tcgatagtag
     8221 acgaagaaat attttatcag tatgatttga cacggagcaa aaagttacca accgctgttt
     8281 tagatactta cattcgggaa acgatgttat ggcattatcg tcctcgtcgt ttgaaaatca
     8341 aagaagaaaa ggcagtaggc taccaaattt cccagatcaa caatgaatgg tttttctttg
     8401 ttggaaagat cgaagtctat gagcgtgatc ggcttttgga acagcagcag aaaatgctcg
     8461 ggcattcctt aacacagtta ctggcgaaat tacaggaaac agcggtgcag caattgccga
     8521 ggaaaagagc tgaagatagt gagcttagtt acctgatgat ccagtatgcc aatgttcttt
     8581 tgatgatcga tttttatatt gcaaaaccag tagtgattgg aatcgattta gaaagtctgc
     8641 cgatttatcg tattgctttt caacaatatc tgatcagaga actgagggga atcggcggta
     8701 ttgagattgg aagttatgaa gaaggtaaag aatatgattt ggtgatcaca ttttgccaga
     8761 gaaacaaaaa acagtgtgaa tattatctgt cagaatttgc gtcaccttac gacattattc
     8821 gattgaaaag aagaatagag acgctgaaaa aagaaaagaa ttgattaaaa aatagcttca
     8881 taataaaaaa ctgtcaaaaa aagaaaaaaa gaataaaatt ttgacagttt ctttttttaa
     8941 aacagtagta tgataagagt agttaaagaa agcgtttgca tataatggag ggaatcacaa
     9001 tgacagaatc aacgtatatc atggcgattg accaaggtac aactagctca cgagcaatta
     9061 tctttgacaa gaaaggaaga cacatcggca gttctcaaaa agagttcacg caatatttcc
     9121 cgcaagaaag ttgggtggaa catgatgcaa atgaaatctg gaattctgta cagtcagtta
     9181 ttgccggagc gttcatcgaa tcagggatca aaccaaatca gattgctggg attggcataa
     9241 cgaaccagcg tgaaacaaca gtaatatggg aaaaagatac aggacgtcca atctatcatg
     9301 cagttgtttg gcaatccaga cagtcagctg gaattgcaga taacttaaaa aatgaaggct
     9361 atcaagaatt tttccacgaa aaaacagggt tagtgatcga tgcctatttc tcagcaacga
     9421 agatacgctg gattcttgat catgtagaag gcgcgcaaga acgagctgaa aaaggcgaat
     9481 tgatgtttgg aacgatcgat tcttggttag tatggaaact gaccgatgga caagcacatg
     9541 ttactgatta ctcgaatgcc agtcggacga tgttgttcaa tatccatcag ctagattggg
     9601 atcaggagat tttagattta ctaaatattc ctcgagtgat gttaccaaaa gtcacttcta
     9661 actcagaagt ttatggttat acgcaaggtt atcattttta tggaagcgaa gcaccaattt
     9721 cagggatggc aggagaccag caagccgcat tattcggaca aatggcattc gaaccaggca
     9781 tggtcaagaa tacttatggt acaggttcat ttattgtcat gaataccggt gaaaaaccgc
     9841 aattatccaa aaataattta ttgacgacaa ttggttacgg cataaatgga aaggtttact
     9901 atgctttaga aggaagtatt tttgtggctg gttcttcgat tcaatggctg agagatggac
     9961 ttcaaatgtt gcaaaaagct agcgattcag aagacgccgc gaaacgctct acaagcgaag
    10021 atgaggttta tgttgttcct gcattcgttg ggctaggtgc tccttattgg gatcaggcag
    10081 cacgaggggc tatgtttggc ttgacacgtg gtacgacaaa agaagatatc attaaagcta
    10141 ctttacagtc gattgcttat caagtgagag atatcatcga tacgatgcaa gatgacactg
    10201 gaatcaagat tcctgtattg aaagtagatg gcggtgcagc aaacaatgaa tatctgatgc
    10261 aattccaaac agatatcttg aatgtaccga tccaacgagc agaaaacttg gaaacaacgg
    10321 cgctaggggc agcattttta gcaggattag ctgtcggtta ttggaaagat acagacgaga
    10381 tccgcgaatt ttatgaagct ggcaagctat ttgaagttca aatggaggaa gagcgccgag
    10441 aaaaacttta caacggttgg aaaaaagcag taaaagctac ccaagcattt gaataaggag
    10501 gaaatccata tgttttcaaa caagacaaga caagatagca ttcaaaaaat gcagcaagaa
    10561 gaattggatc tgttgatcat cggtggcgga atcactggtg ccggtgtggc agtccagtca
    10621 gcagcatcag gaatcaaaac aggattgatc gaaatgcagg attttgcaga agggacttcc
    10681 tctcgctcga ccaaacttgt tcatggcggt attcgttatc tgaagacatt tgatgtggaa
    10741 gtagtagctg acacagttgg tgaacgtgca gtcgtacaaa gtattgcccc acacatccca
    10801 aaaccagatc caatgctttt gccaatctat gagggcgaag gagcaacaac cttcaatatg
    10861 ttctcggtca aagtagcaat ggacctttat gacaaactgg caaatgtgac aggaaccaaa
    10921 tatgagaatt atacgctgac accagaagaa gtattggaaa gagaaccgtt tttgaaaaaa
    10981 gaagggctaa aaggcgcggg tgtctatcta gatttccgca acaatgatgc ccgtttagtg
    11041 attgataata tcaaaaaggc tgccgaagat ggggcttatc tagtaagtaa aatgaaagcg
    11101 gttggctttt tatatgatgg cgatcaaatc gttggcgtca aagctcgtga cctgctgaca
    11161 gatgaagtga tcgaaatcaa agcaaaatta gtgatcaata cgagtggtcc ttgggtagat
    11221 aaagtacgaa acttgaattt tacgcgtcca gtctctccta aaatgcgtcc aactaaaggg
    11281 atccatttag ttgtagatgc gaaaaaactg cctgtaccgc aacccacata cttcgataca
    11341 ggaaagcaag atgggcggat ggtttttgct attccaagag aaaacaagac ttactttggt
    11401 acgacagata cggattacca aggagatttt acggatccga aagtcacaca agaagatgta
    11461 gattatctat tggatgtgat caatcatcgc tatccagaag caaatatcac attgaatgat
    11521 atcgaagcaa gctgggcagg acttcgtcca ctgttgattg gtaattctgg ttctgattac
    11581 aatggtggag ataatggatc gatttcagac aagagcttca ataaagttgt cgatacagta
    11641 agtgaatata aggaaaacaa agtttctcgt gctgaagtag aagacgtgtt gaaccatttg
    11701 gaaaacagcc gtgatgaaaa agcaccttct acgatttcca gaggtagttc tttagaaaga
    11761 gaaccagatg gtttgctgac tttatctggt gggaaaatca ccgattaccg taagatggca
    11821 gaaggagctt tgcgattgat acgccaatta ttaaaagaag aatacggaat agagacgaaa
    11881 gaaatcgact ctaaaaaata tcagatttca ggtggaaact tcgatccaac taaattagaa
    11941 gaaacagtga cagaattagc aaaagaagga gtagcagccg gtttagagga agaagatgct
    12001 acttatatcg ctgattttta cgggactaat gcccgacgta tctttgaatt agcaaaagaa
    12061 atggcacctt atcctggttt gagtctcgct gagtcagctc ggttacgtta tggtttagaa
    12121 gaagaaatgg ttttggcccc aggcgattat ctcattcgtc gtacaaatca tctgttgttt
    12181 gaacgagatc agcttgatga gatcaagcaa cctgtgatcg atgcaattgc tggatatttt
    12241 gaatggacag aagaggagaa ggcgcaacag actaaacgtt tagaagcatt gattgcagaa
    12301 tcagatctgc gggaactaaa gggggagaaa taaatgaaca gtgatatgac tcaaatcatg
    12361 ggggagttta ttgggacact gatcctagtt ttgcttggag atggtgtgtg tgcggctgtg
    12421 aatttaaaca aaagtaaagc acaggcttct ggctggattg tcatcgcatt tggttgggga
    12481 ctcgctgtaa caatggctgt ctatatttct ggattcatgg gcccagctca tttgaatcct
    12541 gcagtctctc tagctatggc gatgacaggg gcaattagtt ggaatcttgt cgttcctttc
    12601 atcattgcgc aagttttagg agcttttgca ggggccattc ttgtttggct ttcttatttg
    12661 ccacattgga atgcaacgaa agatgaaagc gcgattttag gaacgtttgc aacaggtcct
    12721 gctatacgga attatccagc aaatgtgatc acagaattaa ttggtacatt tgtattagtt
    12781 cttgggttgc tagcttttgg acaaaacgaa tttgcacctg gaacgaacgt attcgctgta
    12841 ggtggattga tcttagccat cggtctttca ttaggagggc caacaggata tgcaatcaac
    12901 cctgctcggg actttggtcc tcgattagca catgcggtat tgccaatagc taataaagga
    12961 acttcagatt gggcatattc atgggtacca atcgcaggtc caatgatcgg agcaattatc
    13021 gcggtaggtg tctattcatt aatggtgtaa atctataaaa catactaaag tttgacaacc
    13081 aaaatgtgtc ttgctatagt taagtggaaa ataaataaga cctagaagaa tggggtatgt
    13141 tttgtgagtg tagctggtat ctaatcagta agtcgaatag ccaataaaca aagtttattt
    13201 ggtgtgctta tgaaacggta cctgtcagga aaacaatatc tttgtattct aataaggata
    13261 ggacatgact tttttaagct gtgttctatc cttttattta tctctaggcc cttatttatt
    13321 ttgggacagg tatctcttca agaaaaggag agaatatgga caacgaaatg attagtcaat
    13381 tacaatttga tcggatcaaa aaggaaatcc aagcaagagc aattggaaac tatagcaaaa
    13441 aacgaatcag cgatatgacg gtctcgacaa acctacaaac tgtcttggat cgtcaagaag
    13501 aaacaagaga agcacgcttg atcttagaaa gcagtcagca tgttccattt atggggttgc
    13561 cgaggatcga tgcactgaca gaacaagtca aaaaagggtt gattctgcag ccaaccgatt
    13621 tgatcgaata tgcggatttt cttagaagca gtcggatgat cacaaagttt tttgataaga
    13681 atcaatacca gacacccttg ttgtttgctt atagcaaaca tcttcctgat ttgataaatg
    13741 tagaagagtt gattgaccag aaaataaaaa ataataaagt cagtgacgac gcgtcacgta
    13801 atttgcgtaa agtacgcaaa caattacaac taatcgaaaa agaaatccag tcaaaattac
    13861 tgaaattctt gcgtcatccg aaaaacaaag aaatgatcca agaagcaatg attgttcaaa
    13921 aaggcgaata ctatacgatt cctatcaaag ccagctataa aaataaaata gatggaacga
    13981 ttatcgatga atcaaataaa agaacgacgg tttttattga accaactgtg atttctaaat
    14041 tgaatgaaca ctatcagttg ttaaaagctg aagaaatcag tgaagaatat caagtcctgg
    14101 cggcattgac tggtgctatc gcagaaaacg aagaagtgat cgatttattg atcgaaacga
    14161 tcacggtgtt agatatcatc tttgctcggg caaaatttag ccgagaaatc aatggaagca
    14221 cacctagaat caataaatcg gaacatatca ttatcaagca aggcagacat ccatttttac
    14281 ctgagcatgc ggtaccattg gatgttgaaa tcggtaaaga ctatcgaggc ttgattatca
    14341 ccggagcaaa tgccggaggc aaaacagtcg tgttgaagac agtcggttta ttgactttga
    14401 tggcgatgtt cggtatgcaa gttccggcaa aagaagggac agagcttgct gtctttgatg
    14461 agattttcgt cgatgtcggt gaccaccaaa atttagaaaa tgcgttgagt acattttctg
    14521 gtcatatgca aaacatcgct gctattttga aaaagataaa aaggaatacg ttggtacttt
    14581 tagatgagat tggcagtgga acagagccaa atgaaggggc tgcactagcg atcgcaatca
    14641 tggaatctat gtatgagcaa ggggcattga tcattgccac gacccattat ggagaaatca
    14701 agaaattcgc cggagatcat gaagattttg tgccggctgc tatggctttc gatcgggaag
    14761 cacttcgtcc taaatatcag ttgcgggttg gagaaacagg agaaagccaa gctttgtgga
    14821 ttgctcataa aatggatatg tcgatgaaat tgatccatca agcagagcgt tacttggcgg
    14881 aaaaagccta tcggacacag aaaaaagaat ttcttcctaa gacagcagtt acaaatagga
    14941 aggagaataa aaaagaacgc cagctctttg caaaaggtga tcggatattt gtcaatgaat
    15001 accaaaaaga agcgttagtt tatgaagata taggtgaaga tacgattgat gtatatttag
    15061 acaaaaaaat aatccacgtg cctcgtcagc gtgttcgtct ggtgcgttca gcagaagatc
    15121 tgtacccgac aggttacgat ttagacagtc tgtttatcga ttataaaaca cgtaaacggc
    15181 agcgtgacct agagcgagga tcaaaaaaag ctcataaagt actagtaaaa gaaatgcgca
    15241 aacgacagga agaaagaaga gtaaacgatg aaaacagcaa gtgattatct caaattagct
    15301 cataatatca gtggctgtaa agaaaaataa aaacagtgaa atcattggtt ttattccccg
    15361 tgtaagctaa aataaaaaga aaagagtgct gaggaggtag ggcgataaaa tgaaattaag
    15421 cacagactgg cgtaaagaaa tacaaacgat tccgaatctt ttatcgatat tccgtatttt
    15481 tttgctgccg atttatcttt attttgtgct gagacagtca ttttatatag caggagcggt
    15541 cattgttgtc tcaggtttgt ctgattatct agacggggtc attgctcgga gatataatca
    15601 ggtaacagac ttagggaagg tgcttgatcc gtttgcagat aaattgacac aactttttct
    15661 gattttgtct atggcatggt atcgaccgtg gctttggctg ttgtttggtc tattcttgat
    15721 aaaagaaggc tttatgtttg tagctggatt aatcgggcta tctaaaaata tcaaattaag
    15781 tggagcgaag tggtatggca aagtagcgac tgctgtcatc tatgttggca tgatcctttt
    15841 gctgcttttc ccagaattac caacgctgtg ggtgagagtc atttttgccg tcatcactta
    15901 cggtctttta caatcttttg tcttgtatgc tgtcgagtac cgaaagatgt ttcaaagaaa
    15961 ataaaaaagg ggtgtgacct atgtcgcatt cctttttttg ataaatagac tcccagtagc
    16021 tacttctcga taataagcca accaaaccaa aaacaggaga agttgttttt gaaattattt
    16081 cttgtgattc ggagttaaat ggtaggctca caacctcttg gtaaacggtg ttcaatcagc
    16141 tgaccttcgg tattagctca aaaaacggca aaatatgagg agctattttc ccgttttttt
    16201 ctctaatact taggtcaaac cgctgatttc acaacctctt ccttaaacgg tgttcaaaag
    16261 cttgcatctg cggtaataag cccaaccaaa cgaaaaatat gagaagctat ttttgtttgg
    16321 ctgttcttat tacttgatgc aggacagcgt tttcacaacc tttgattcac ggttttccag
    16381 aagtagcttc ttcgaaaaat aagccgaaat ttcttgaagc tgatcacttc tgtcgcaacc
    16441 tctactgact aaggcttgat ataagcataa ccttgggatt gcagttcaac taaatcaagg
    16501 acacctgctg gtacaacgat tacttgttca ggtaaagaac tttctgaaat atgattagca
    16561 cgcatagcat tggcgcaagc gtgaaaaacg actccttgca aatctaagaa ttctgcattg
    16621 gcagagtcta gataaccggt aatcgcgatg ccatttacag atacgacaat cgtcgtttct
    16681 ggagaagctt ttatcaggtt ttttacgttt ttccctgttt ctgaccattt ttctaattca
    16741 tcaatatgga aaacgacttt cattgaaatc attcctttac tttttaataa tcatcttgat
    16801 cgcttgttct agtttagccg cttgttcttc tggattacta tcaggtttct caaagcaatt
    16861 tttcagatta tctgcaacga tcatgcccat tacgcgatca atactagaac gaacagcgct
    16921 taattgagtg atgacatcca tacattcttt ttcgtcttcg atcatttttt ggataccacg
    16981 aatttgtccc tctgtacgtt tcaaacggtt caggatattt ttattatcaa tctccataac
    17041 acattctcct ctatttttat aattagataa taccccttat cggtatttta tgcaaatata
    17101 aaaagtagtg aaaaaaatga agagtcattc tggttgacat attaataaag ctcctgttta
    17161 atatacccca tgaggtattt ataaaaatgt aaatgaggtg cagaaaatga atttgctatt
    17221 tcaatcaata aaatcgataa cagtcccaga attaaaggaa aagcttttgg aaaatccagc
    17281 gttacttgat gttagaacac ctgcagaata ccggggcggg catataaaag gagctaaaaa
    17341 tgttccatta caaagtataa acagatatga tggagacaaa gaaaagaccg tctatgtgat
    17401 ttgtcaatca gggatgagga gtaaacaagc agctaaagaa ttaaagaaat ctggttatga
    17461 tgtagtgaat gtacgtggtg gcatgaatca atggtttgat cgaacagtag gagggaaata
    17521 agatgaaaat cgttattgtc ggaggtgtag caggtggaat gtctgctgct acacggcttc
    17581 gccgattaat ggaagatgca gaaatcgttg tttttgaaaa aggaccgtat gtttcttttg
    17641 caaattgcgg cttgccttat tatctttcgg gagaaatcag tgaacgggaa aatcttcttg
    17701 tccaaacgcc agaatcatta tccgctcgtt tttgtttaga tgtgcgtcca aatcatgaag
    17761 tgacagccat ctttcccgaa aacaaaacgg tagaagtcgt acatgagggt caaaaacaca
    17821 ttgaacagta cgatgcattg gttttatctc ctggtgcaaa accagttgtt ccatcgattc
    17881 cagggataac agaagccgac aatgtttttt ctattagaaa tgtaccagat atcgataaag
    17941 tgatgcatgc attagaaaaa cagccaaagc gtgccgtgat cgttggtgca ggattcatcg
    18001 gattggaaat ggcagaaaac ctaaaaagaa gaggtttaga agtcatggtg atcgaacaag
    18061 caccacatat tcttccaaca ctggatgaag aaatggcagc ttttatagaa aaagaattgt
    18121 ctcatcaagg agtagaagtg attacttctc atgctgtcgc tggatttgaa gaccacggga
    18181 aacgattgcg actggatgat gggcgtacca tccctgctga tttagttatt ttatccattg
    18241 gtgttcgtcc tgataaccag ctagcagtga ccgctggaat cgaattaggc atacgcgggg
    18301 gtatcctagt agacgaacgt tatcaaacga atattcctga tatttatgcg gtgggggatg
    18361 ctatcgttgt aaaacagcaa atcactggaa aagatgcact tatttctctt gcttcaccag
    18421 ccaatcgtca aggtagacaa gttgcggaca cgatttccgg aatttctcga agaaatcaag
    18481 gcggtattgg aacagcaatt atacgaacgt ttggaatgac tgccgcatcc accggtttaa
    18541 gtgaaagaac agccaaagaa aacgaactgt cttttgaagt cattcatgta tcaggaaaag
    18601 atcatgcaag ctattatcca gaagcaacag atattttact gaagttgatc ttccatccag
    18661 agactggcga gatttatggt gcacaaggtg ttggggcaaa aggtgtggat aaacggatcg
    18721 atattttagc aacagcaatc aaagggcatt tgacgatctt cgatttaccg gaattagaat
    18781 tgacgtatgc accgccattt ggctcagcca aagatccagt aaacatgcta ggatatgcag
    18841 caatgaacat tgcagaaggg cttagtgaaa ccgtacaatg gcatgaattg ccgacagaac
    18901 tagcaaaagg aaaaatttta ttagatgtgc gaacagcaga agaattggaa aagggcaaat
    18961 tcaaggaagc caaacatatc cctttgaatg aacttcggga ccgattaaat gaattagaca
    19021 gccaacaaga atatatcgtc agctgtcata gcggattacg tagctatcta gcggaaagaa
    19081 tcttgaagca gtctggctac cacgtaaaaa accttgatgg tgcattttct ttatatcaaa
    19141 ctgtccgacc tgaagaactg atatatccta acaaatgata ctcgttaaaa agcttgcatt
    19201 cgactcttaa aagaggtaaa ctaatcggtt ccaccacttt aaaaaaagag gagaatgtgt
    19261 atggtcaaca cacaaaaaca gttagaagaa gcatatctga agaacgtgta tcaaaagcta
    19321 attgataaaa aaaacgaact gcgggaatcg atggatacgg caaaaataga aggaaaccag
    19381 tcgttgaaag aaatggctgg cgagcttcga ttgaattttg acagttactt agataacctg
    19441 gatacttttt cgatgattga aatgaaaaat agagaaatcg atcagatgaa tatcaaacag
    19501 aaaaacgcag aaaatcagct agcaaagatc gaacgtttat tgaaaagtcc ctactttgga
    19561 aaagtcgttg tagactttct ggataatgag ccaaaagaat ccttttatat cggtacagca
    19621 aatttcacag atgatgcaga tgaaaatctt gtttatgatt ggcgttcacc aattgccgaa
    19681 cttttttaca ataacatgat tggtccatct tcttatacgg ttcgtcaaaa taaaatcgat
    19741 acggttatta aagaaagacg acaatttctt ttggaaagag atcgtttggt caagtttttt
    19801 gatacgtctg tagctattca agatgacgta ttattagaag tcttaggcca agatgagacc
    19861 aatgaaatga aggctatcac tgctacgatc caatcggaac aaaacacaat cattcgggat
    19921 gtgaaaagtc aaaatatctt ggtaaatggc gtagcaggaa gtggtaaaac ctctgctatc
    19981 atgcagcgta ttgcgtatct gctctattta tatcgagata cgatgacatc cgatgacttg
    20041 ctgattctat cacctaatca tcggtttatc gactatattt cgaatgtcct accatcatta
    20101 ggtgaaagaa atccactgaa tctaaccatc atacagctgg tttcacagct ttcagcagag
    20161 gaaatagaag gtgaagaggc ttatttcaaa cgtatcacgg gagaaaatgt atcggagcag
    20221 acagaacgtt tgcgtagcaa aaagtttatc gataatctaa aacaatcaga tcctttgttt
    20281 ttagatcatc cgaattttat ccgtggactt actaaaaatg gaaaaacagt cctttctaaa
    20341 aaaacgatcg aaaaaatata tgagaaagtt cctgctcatc ctaaattgat cgataggctt
    20401 caagcaacga aaaaggcact gatgagcgaa tggaagaatc atcttttgaa acaagcaaaa
    20461 agtcctgctg tgcagaatca ggtactctcg ttgacagaag ataggcagtt ggaattattc
    20521 ggtaaattga tttctgatga ttcggaacaa agtattgtag cttatgcaag gaaactttta
    20581 caaaaaaaat atcgaaaaat aaccagacaa atcgaagaaa tggcatgggt ggatgagcat
    20641 cagttgtttg aaagaattta tgaaaaacga tatggctcag cttatgcttg gcaacccaca
    20701 cgtactgttg atgaagcagt cattatatta gctattcgtc atttacttgt tgaaaaggta
    20761 aatgtaccag catttcgcta tctattgatt gatgaagtac aagattatac cctggcacaa
    20821 ttaggtctat tgatcgaatt atttccaaaa actcacttta ctttagtagg tgacgagaat
    20881 caggcaatct ttaattcttc cactactttt gctgatatta tgcgttgttt cgatgactac
    20941 catctgccga tccatcggta tgatcttaga aatagttacc gttctagcgg agcaatcaca
    21001 gagcttttca aaacctatgc tgtggatcaa gagaagatcg acatcgtatc gatccgaccg
    21061 caaggaaaag aacccgaata ccgctctttt cgatcctctg aagaattgtt gaaaatacta
    21121 gctgagattt tggtttctct aaaaggagaa aaagcggcag ttatcaccca aacagaagag
    21181 gaaacccaaa cgctacaaga atcgattaaa aaaaattcat atggattaga aaactgccaa
    21241 atcattcctt tgagtctagc aaaaggactg gaatttgatc atgtaatctt atatccattt
    21301 gaaaacgatg gagatgagca aagaagacgc agacaaatgt acacggcgat atctagagga
    21361 atgaaaagca ttgtcgtatt ggaaagagca acataattaa gagataaaaa ataaaaagaa
    21421 caactgataa aaagaacaaa atatttttca tttgctgatg agaaatgttt tgttcttttt
    21481 cttaggaaaa atttaggaac actttatgtg aaaagaaatg aaatgtatgg taaactccta
    21541 cgtcgtaaca aaaaaacgtg aggtgaggat atgaaaaaag taacaagaga agaagttgca
    21601 tcaattttgg tgaaagataa gtatttttca aaaggtaatt actggttaaa gatttggcaa
    21661 acgttagtgg ctcttttagg atggatgatc gttgttcttc cttttatatg ggtctttttc
    21721 ccattaacga gaccagaaag agccaaggat ttttccttgt acgctttttt atcagaaaag
    21781 aaaatgctgt atttcttaat cggctttttt gtattgattt ttttcagttt cctgattacc
    21841 tcatttgtat tgactaggtg gaacaatcga aatctataca aaaaagtaaa taaatctttt
    21901 gaat
//
LOCUS       DAGCYL010000027        21225 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.027, whole genome shotgun sequence.
ACCESSION   DAGCYL010000027 DAGCYL010000000
VERSION     DAGCYL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.027"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            332..946
                     /gene="gmk"
                     /locus_tag="MB216_000811"
     CDS             332..946
                     /gene="gmk"
                     /locus_tag="MB216_000811"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBT4525669.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            951..1256
                     /locus_tag="MB216_000812"
     CDS             951..1256
                     /locus_tag="MB216_000812"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBT4525670.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1509..3917
                     /gene="priA"
                     /locus_tag="MB216_000813"
     CDS             1509..3917
                     /gene="priA"
                     /locus_tag="MB216_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBT4525671.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3932..4423
                     /gene="def"
                     /locus_tag="MB216_000814"
     CDS             3932..4423
                     /gene="def"
                     /locus_tag="MB216_000814"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBT4525672.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4416..5354
                     /locus_tag="MB216_000815"
     CDS             4416..5354
                     /locus_tag="MB216_000815"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBT4525673.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5354..6712
                     /gene="rsmB"
                     /locus_tag="MB216_000816"
     CDS             5354..6712
                     /gene="rsmB"
                     /locus_tag="MB216_000816"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBT4525674.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6725..7465
                     /locus_tag="MB216_000817"
     CDS             6725..7465
                     /locus_tag="MB216_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBT4525675.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7462..9531
                     /gene="pknB"
                     /locus_tag="MB216_000818"
     CDS             7462..9531
                     /gene="pknB"
                     /locus_tag="MB216_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBT4525676.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9696..10595
                     /gene="rsgA"
                     /locus_tag="MB216_000819"
     CDS             9696..10595
                     /gene="rsgA"
                     /locus_tag="MB216_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBT4525677.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10608..11258
                     /locus_tag="MB216_000820"
     CDS             10608..11258
                     /locus_tag="MB216_000820"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBT4525678.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11259..11897
                     /locus_tag="MB216_000821"
     CDS             11259..11897
                     /locus_tag="MB216_000821"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBT4525679.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12052..12906
                     /gene="mreC"
                     /locus_tag="MB216_000822"
     CDS             12052..12906
                     /gene="mreC"
                     /locus_tag="MB216_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBT4525680.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12910..13419
                     /gene="mreD"
                     /locus_tag="MB216_000823"
     CDS             12910..13419
                     /gene="mreD"
                     /locus_tag="MB216_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBT4525681.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13674..15212
                     /locus_tag="MB216_000824"
     CDS             13674..15212
                     /locus_tag="MB216_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBT4525682.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSATEE
                     STTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
     gene            complement(15280..17079)
                     /locus_tag="MB216_000825"
     CDS             complement(15280..17079)
                     /locus_tag="MB216_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBT4525683.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            17202..17708
                     /locus_tag="MB216_000826"
     CDS             17202..17708
                     /locus_tag="MB216_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBT4525684.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            17787..18509
                     /locus_tag="MB216_000827"
     CDS             17787..18509
                     /locus_tag="MB216_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4525685.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18587..19987)
                     /locus_tag="MB216_000828"
     CDS             complement(18587..19987)
                     /locus_tag="MB216_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBT4525686.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(20157..21131)
                     /locus_tag="MB216_000829"
     CDS             complement(20157..21131)
                     /locus_tag="MB216_000829"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBT4525687.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
BASE COUNT         6803 a         3989 c         4534 g         5899 t
ORIGIN      
        1 tattaagcca aatttatgca aaaatgtgag aaactatttt cacaaaattt ttcttcatac
       61 tcgaaactaa tcggcttctt cacgacctct tatacacggc gttcaaaagc ttgcatctgc
      121 gataataacc ccaaccaaac gaaaaacatg agaagctgtt tatgtttggc tgttcttatt
      181 acttgatgca ggacagcttt tttacgacct cttggtacag ggtagctttt ttaccaattt
      241 ctagctataa ttaaagataa ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa
      301 gtacaaaaag actagttgaa aggacgtcgt catgtcagag cgtggattat taatcgtgtt
      361 gtctggtcca tccggtgtag gaaaaggcac agtacgaaaa gcaatttttg agagtgagga
      421 taatgatttt cagtattcga tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga
      481 aggcgttgat tatttcttcc gaagcaaaga agagtttgaa tcaatgatcg aagctggaga
      541 gatgctggaa tacgcagaat atgtcgggaa ttattatgga actcctttgt catatatcaa
      601 caagacattg gatgaaggaa aagatgtttt cttggagatt gaagtccaag gagcaaaaca
      661 agtcaaagaa aaagtgcccg atggtgtatt catctttttg actccgccag atttagcgga
      721 attaaaatca cggatcgtag gccgaggaac agatgcagat gaagtaatcg aagaacgaat
      781 gaaagttgca aaagaagaaa tcgaaatgat ggctctttac gactatgctg ttgtaaatga
      841 tgaagtgccg ctagcagttg aacgaatcaa aaacattatt gccagtgaac attttcgagt
      901 agatcgcgta atcggtaaat atattaaaat gttaaaggag atgtagtctt atgatgttaa
      961 aaccttcaat tgactcacta ttagatcgtg taaattcaaa atattctcta gtgattttag
     1021 caagtaaacg cgcgcatgaa ttggatgcaa gtgcacagcc tactctagat tcttttgatt
     1081 cagtaaaaag cgtcggtcaa gctttagaag aaatcgatgc aggaaatgta gtcaatgatc
     1141 cacatcctga attaaaacgt gaacgtttga agatggaaga agaggaacgt caagcacaaa
     1201 aagagcgtga acaacacgaa ttggaatcac gtatccgtga agagcaaaaa atgtaataaa
     1261 gaaaaataga gcgaacatct aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt
     1321 cgccacttat tgtctggtag aaaaggctgt gtctgaactt tttgacacag ttcttttttt
     1381 tcttatctgc tgttaatttt ttgttaggat ttggcagaag ataagacaaa tccatcgatc
     1441 tctagtaaaa taggcaagga actttttgtc gcttgataag aaaagacaaa aaaaggagga
     1501 aacgaaccat gacattaaca gctgaagtca ttgtggacgt acctactatg cagaccgatc
     1561 agccattcac ttatttggtt ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag
     1621 tagaagtccc ttttggtaat ggcaatcgtc atgtccaagg atttgttatg gctatccaaa
     1681 aatcagaaga atcagctaat ccatctttga aggcagttat tcgtttgcta gaccttgctc
     1741 cggtggtcaa tgaagaatta ctttcattag ctgattatat gaaaaaaatt acttacgcat
     1801 tcaaaatcac ttgtctacaa acgatgcttc cgagtgtgat gaaagcagaa tatgataaat
     1861 tgatctatcc tttggcagac acaccagaag ttcaggtatt gtttgatcag cgagaagtga
     1921 tttcctggaa ggaagcagaa gaagaaggca gcctttctca attgattcgc tggcgccaag
     1981 agcaattagt agatatcaaa tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt
     2041 tggtccgttc tttgttaact gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa
     2101 atgccaagaa acaaaaagaa cttcttttgt gtttatctga actgagccaa gaagaaccaa
     2161 ttgcttattt caaaaataaa ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg
     2221 gctggctgga gtttatagaa tcagaacgtt atcgcgaccc ttataaagac cgagtatttg
     2281 accaaacgac ggcattggaa ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca
     2341 cagcaggtca gcagcaaaaa gatcaggtct ttttgctgga gggaatcact ggcagcggga
     2401 aaacagaagt atatcttcag gcaatcgctg atgttttgtc agaaaataaa acggctatta
     2461 tgttagtgcc tgaaatcgca ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg
     2521 gagaatcagt tgcggtctta catagcggac tttcccaagg tgaaaaatat gatgaatggc
     2581 gcaagattga gcgggaagaa gcgcaagtag tagtaggcgc acgatctgct atttttgccc
     2641 cgctgaaaaa tatcgggctg atcattatcg atgaagaaca tgaatcaagt tacaaacaag
     2701 atgagacacc gcgttatcat gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt
     2761 gcccaatagt tctaggaagc gcgacgccct ctttagaatc aagagcacga gctcaaaaag
     2821 gcgtttatca gttattgcag ttaaaccacc gagccaaagc agctgcacag cttccagcta
     2881 ttgagatcgt agatatgcgt gaagagtttc agaatcatcg gacctctact ttttctgcta
     2941 atttgcagga aaaaatacaa aatcgattag acaaaaaaga acaaactgtc ctactgctca
     3001 atcgtcgagg atattcgtct tttgtgatgt gtagggactg tggatttgtt cttccatgtc
     3061 ctaattgcga tatctctctt acactgcata tggatacacg ttcaatgcgc tgccattatt
     3121 gcggacatga agaacctatt cccaatcgct gtcctaattg tggcggcaat aaaatccgct
     3181 actatggaac aggtactcaa aaagtagaag aggaactccg agaactttat ccgcaagcta
     3241 ggattttgcg tatggatgta gataccactc gaagaaaagg ggcacacgaa cagattttac
     3301 aaaaattcgg tgcaaaagaa gcagatatat tattagggac acaaatgatt gctaaaggtt
     3361 tggactttcc agaagtgaca ttagttgggg tgttgaatgc tgatacctcc ctgaatttac
     3421 cggattttcg ttctagtgag catacattcc aattgcttac ccaagtttca ggacgagcag
     3481 ggcgagcgga aaaagcaggc gaagtcgtga tccaaacatt caatcctcaa cattacgcca
     3541 tagaattagc taaaaaacaa aattatgagc agttttatca gcaggaaatg catgtacggc
     3601 atcgtggagg ctatcctcct tattacttta ctgtgaaaat aaccgccagc catcctgaag
     3661 aacaagtagc ggcaaagaaa atctttcaga ttgccaatca gctaaaagaa gtgttaagcc
     3721 ctcaaagctt gctattaggg ccaacgccca gcatgatttt gagagtgaaa aaccgatatt
     3781 attatcaact aatcatcaaa tataaacatg aaccgaatct tcctcatgtt ttggatgaaa
     3841 ttttgaatgg aagccagaaa gaacagcgac aaggtctttt tgtggcaatt gacaacgaac
     3901 cattaaactt tatttaagaa agggccaaat tatgcgctat cctatattga tccaccctaa
     3961 tgataaatta aaaagaactg ctcagccaat cgatgtaatc acggacgaga caatcgcttt
     4021 attagacaat ttatatgaga caatgattgc gaatgacgga atagggatcg ctgctcctca
     4081 agtaggacaa aacaagagaa ttgctgtgat cgaagtagat gaaggcgaga aatttgaact
     4141 gattaaccca gaaatcatcg aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag
     4201 tattccgcat gtctatggaa cggtcaagcg agcagatgaa gtgaccgtgc gttattatga
     4261 tcgagacgga gaagagatag aagtgacggc ttttggctat ttagcccgtg ccttccagca
     4321 tgaaatcgat cacttagacg gtatcttgtt cattgagaaa atgatacagc agatcccgga
     4381 agaagaactt gaagaatata tggaggaaca tcttgatgac taagctagta tttatgggca
     4441 cacctgcttt ttctgtgccg attcttgaag ggctgataga atcaggttac gagatccagg
     4501 cagtggtaac acagcccgac cgtccagttg ggcggaaacg agtaattacg ccgacaccgg
     4561 taaaagaagc ggctctgaag cacggtatcc gtgtgctgca gccagagaaa atctccggtt
     4621 cgccagaaat ggaagaaatc attgaattgg tgcctgatgt gatcgtcact gcggcatttg
     4681 gccaattctt gcctgaaaag ttattgcaag taccaaaatt aggggcaatc aatgtccatg
     4741 cttctttact tcctaaatat cggggaggtg cgcctgttca ttatgccatc atgaatgggg
     4801 agaaagaaac tggtgtaacg atcatggaaa tgatcaaaaa aatggatgca ggcggaatct
     4861 atgcgcaaga aagcatacca atcactaaac aagatgatgt agggacgatg tttgaaaagc
     4921 ttagcctttt aggaagaaag ctattgctag aaacattgcc caatattttg gatggacaaa
     4981 aaccagtacc tcaagacgag tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag
     5041 aagcaatcga ttggcataaa actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc
     5101 cttggccaat tgcattcacg acctatgagg gcatacgctg gaaattatta tcggttgaag
     5161 tattggcaga aactacagac aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc
     5221 tatggattgc ttgcggtaaa cagaccgtct tagccatcaa tgaattacag ccagctggta
     5281 aaggaaaaca ggccgtacat gatttcctta acggaagcgg tcagcaagtt cagattggtc
     5341 agcaggtgaa ataatgaaaa aaatccctaa aaaaatgaaa aaatcagtac gatatatggc
     5401 attactgaca ttagagcgag tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga
     5461 gatgatcaaa caaagcgagc taaatcaaaa agatgtgggt ttatttacag aattgatcta
     5521 tggtacgatt agccatcagc gactattgga attttacgtt gcaccattga tcgctaaagc
     5581 aaaaaaagtg gatgactggg tcaagacgct tctttatctt tctgtatacc aattagaatt
     5641 tttagataaa gtccctgcac atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa
     5701 aggaaatcct gggacaggga aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg
     5761 agcaccagat ctgcaaggca ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct
     5821 gcctctatgg atgacgaaac gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct
     5881 cggtttatcg ctatatcagc ctagccatgc tagtgcccga gtagatacac gccgactttc
     5941 cagagatcag gccatcactg ttttgcaaga agaagagatc acagctgaaa aaagccaagt
     6001 cagtccatac ggtatagtag cgaaaaaagg gcatttagct tctagctcat tgttccacga
     6061 tggcgtcatg acgatccaag atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga
     6121 gcctgaacat catgtcttag atgcctgtgc agcacccgga ggtaagacga cgcatattgc
     6181 ttctttttta gatccgtcat gcggtggaga agtggtcgct ttagatgtac acgaacacaa
     6241 agtcaaactc attgaagata atgcagcacg tctaggtgtc tcttctgctg tctacgctca
     6301 aaagatggat gctagagaag tcagtaaaca gttcgaagca gagcagtttg atcggatttt
     6361 agtagacgcg ccttgttcag gtcttgggct gcttcggcga aaaccagata ttcgttataa
     6421 aaaacagccg gatgaattag cgaatttacc aaaaatccaa ttggaaattt tagaaagctg
     6481 cgcaccaact ttaaagtctt ccggtattct gacttatagc acttgtacga tcttaaaaga
     6541 agaaaaccaa gacgttgttt ctgctttctt ggaaagacac cctgattttg agaggattga
     6601 tgttttgacc aatcctatgc tacaatcatc aatcgaagag aaaatgttga ctttgtatcc
     6661 gcatcagttt tacacagatg gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga
     6721 aaaaatgcag attgaatatc aatcagatgt aggaagacga cgtaacacaa atcaagacta
     6781 tgccagtgta tttaccagcc gttcaggtct caagcttgct gtattagcag acgggatggg
     6841 tggtcatcgt gccggagaca ttgccagtca gatggcggta acgaatctag gcgaagcttg
     6901 ggaaaaacaa gaagctgttg acgatgaaaa aatcgcccaa tggttcattc aatcgattca
     6961 agaagaaaat atgctgattt atcagcgtgg acaagaacag ccggaatata acggtatggg
     7021 aacaacaatc gtagcggccg ctctgtcaga tgactggttc acgattgcca atgttgggga
     7081 tagcagagct tatttgattc gtgaagataa aatcatgcaa ttgacggagg atcattcttt
     7141 agtcaatgaa ctagtcaaat caggagaaat cagcgaagaa atggcagtga accatccaag
     7201 gaaaaatatt ttaacacgtt ctgtcggcat gcctggaaca gtcgaagtag atgtttctac
     7261 tcatctttgg caactaaaag atcgactgct gttatgttca gatggcttga cgaacatgct
     7321 gtctgaagaa gaaataggaa cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac
     7381 tgagctgatt gatcgtgcca atgaagcagg cggagcggat aatatcaccg ttcttcttat
     7441 cgaatataag gaggacagtg tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa
     7501 atcactggaa atatcggtag cggtggtatg gcaaatgtat ttttggcgca cgatttaatc
     7561 ttggatcgtg atgtagcagt taaagtctta cgatttgact ttcaaaatga ccagactgcg
     7621 attcgacgat ttcagagaga agctttagca gcaacagaac tcgttcatcc aaacattgtt
     7681 agtgtttacg atgttggtga agaagacaac atgcagtatc tggtgatgga atacgtcaaa
     7741 ggaatggact tgaaacgata tatccaaaca cattatcctg ttccttatga aacagcagta
     7801 aatattatgc agcagatcct ttctgctatt tctttagctc ataaccatca aatcatccac
     7861 cgagatttga aaccccaaaa cgtactgatc gataatgaag gtgttgtaaa aatcacggat
     7921 ttcgggattg ctatcgcttt atctgaaaca tcgatcacgc agacgaatac gatgctggga
     7981 tctgttcatt atctgtcccc tgagcaggcc agaggaagta tggcaacgaa gcagtcagat
     8041 atttatgctt tagggatcat cctttatgag atgttgacgg gtagcgtacc gtttgatggt
     8101 gaatctgctg taacaattgc tttaaaacat tttcaagatg atcttccatc tataaaagca
     8161 cttgatccaa atgtgccaca agcactggaa aacgtgattt tacgggcaac ggctaaagaa
     8221 cctgcagacc gttataagtc agccgaggaa atgtctgatg atctttctac agtattatca
     8281 ccagctcgtg caaacgaaga aaaatggcag cctcatgtga tggataatga aacaaaagtt
     8341 atcacacccc ttacggaaga tgcaccaatg cctgattcat tcaaatctat gcctctgcca
     8401 aaagacaagc cagaaaacga aaccttgcca gaggaggaag aagttctgcc tgatcctaaa
     8461 aagaaaggac agaaaaaatg gtggctgatc ctattagcta ttcttgctgt tttaggaatc
     8521 ggaacagctg tattctttgc ttctggcggg cgaggagaag ttgttgttcc tgatgtttct
     8581 gatctttctg aagcttctgc tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca
     8641 aaaacagaag aaattgcaga tgacaaaatc gaagaaggta atgtagtgaa gacagacccg
     8701 gcagcgggaa cgacagtcaa gcaaaaacgc gaagtcactc tgtatattag tagcggcacg
     8761 aaaaaaatca aattagatga ttacagtggt aaaagttatg aagatgccga agcaagtttg
     8821 aaagaattag ggttttctgc tgacttgatt caaaaagaag aggaattcag ttcagaggta
     8881 gaaagcggaa tgatcatttc gcaatctcca tcagaagata cagaagtcga tcctaaaaca
     8941 gacacgatta ctttcacagt cagcaaagga ccagaaacag tgactttgag tagtcatgta
     9001 ggagaaaact acgaaactgt tcgtaattgg cttgttacac aaggattcaa tatcaataat
     9061 attagtgcta cttatgacta tagcgacact gtaccatcag gttcgatcat cagtcaggat
     9121 ccagcctcgg gtgaagttgt agcagaagaa acttacatca attttgtcgt aagccaagga
     9181 acagagccta aattgaatga tatctccggc tatacaaaaa gcgaagcaca aagttattta
     9241 gctagtgtcg gtgcagaata tatcgggcat gagacttccg aatattcaaa cacggtggat
     9301 aaagataaag tgattcgtac agtaccaggg gcaggaacga caattaccaa aggaatggtt
     9361 gtgaacgtaa tttactctaa aggaccagat ccagcgtctt ctagttctga aaaagactct
     9421 tcagacagtt ctagcagctc aagtagctcc agtagcacaa gcaactcttc aaaagaagaa
     9481 tcttccagct ccacaagttc atcagctagc acctcagaaa gttcaaaata agtgctgaat
     9541 aaaatatatc agaagggaat gcgacattga tttgttgcat tctctttttt gtgtttctat
     9601 ctcttttgat caattttcaa gccatatact ttgagaagtt tgcatggaat aacacctact
     9661 ttcctcttcg tatggtaaaa taaacaaaaa cggaggtggc acttatcaaa ggacaaatca
     9721 gaaaagcaat cagcggtttt tactatgtat acgcaaacgg agagacctat caaacaagag
     9781 gaagaggaaa tttccggaat cggaaaatca cgccgcttgt cggagatcag gtgatctttg
     9841 agagtgaaaa tcaaacagac ggctatcttc tggaaatctt gccgagaaaa aatcaattag
     9901 ttcgcccacc agtcacaaat gttgatcttg gaatcattgt cacaagtatc gtagagcctg
     9961 cattttctta taatctactt gaccgctttc tagttacctt ggaatacgag tcgatcgaac
    10021 cgattatttt tttgacaaag accgacttgt cagacgaccc agaaaaaatc gaagagatca
    10081 agcaaaccta tactaaaata ggctatccgg tgattgtacc caatcatacg ggtgatacga
    10141 cagaactgat ccgttacttc ccagaacggc tatcggtctt tatgggtcag tctggagcag
    10201 gaaaatcgac gctactgaat caaatatcac cggaactgaa tttagcaaca ggagagattt
    10261 ctgatgcact tggaagagga cggcatacaa cacgtcatgt ggaactgatt cctatctatg
    10321 gcgggttagt agcagataca ccaggattta gctcgattga ctttttgaca attgaagcag
    10381 gagaactgcc gagacaattt cctgaatttg tagaagcttc tgtccattgc aaatttagag
    10441 agtgcatgca tgctaaagaa cctggctgcg aagtaaaaca tagagtagaa acaggtggta
    10501 tcgcacagac acgttacgac aattatttgc aattcttaca agaaatcgaa aaaagaaaac
    10561 caatgtatgc aaaaaaagaa ggtaaacgaa aataggagga aacaagaatg aagatagcac
    10621 catccatttt gagtgcagat tttgccaatt tacaaagaga cattgagtta gtggaaaaag
    10681 gaggcgcaga ttacatccac gtagatgtaa tggatggcca atttgtccca aatatcacat
    10741 tagggccaaa tattgttcag gcaatccgtc ctgttactaa attaccgtta gacgtccatc
    10801 tgatgatcgt tgatccagaa aactacattc cagcatttgc aaaagctgga gcagatatca
    10861 ttactgtcca cgtcgaagca actcctcata ttcatcgggc acttcaaatg acaaaaagct
    10921 taggagttaa agctggagta gttatcaatc caggaacacc agtctccatg atcaaacatg
    10981 ttttgccttt ggctgatcaa gtactagtca tgactgtcaa tcctgggttc ggcggacaaa
    11041 gctttattga agaaacagta gaaaaaatta cggaactcgc tgatctacgt agagagaaca
    11101 attggactta tgcaatcgaa gtagatggcg gaatcgttcc agaaacagca aaaatctgta
    11161 aagatgcagg ggcggatgtc ttcgttgcag gatcatatat ctatggagcc gaaaatccag
    11221 tcggacaaat cgaacgatta aaagaggcat tagactaaat gaaaatttta ttagctgctg
    11281 gaggtaatcc gcaaaactgg ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga
    11341 tagaccgtgg tgcgctgtac ctgcttcaag caggctaccc agtagatctg gcagtgggcg
    11401 attttgattc attgtcgaac gaagagcaga aggaagtatt tgcaaaagcc aaagagtata
    11461 cccgttcccc tgcagaaaaa gacgatacag atacacagtt aggtatctta aaaacatttg
    11521 aacgttaccc gcaagcggaa gtcactttga ttggagctac gggcggaaga ttggatcatc
    11581 ttttagccaa tctctggtta ggtatggaac ctcgcttcaa accctttgcc catcagctta
    11641 ttatcagaga ttgtcaaaat tatttgtctt attatttgcc tggagagcat atagtaagac
    11701 agataccaga tatgaagtat ttagcttatt gctgcttgac tcccgtctcg aatcttacat
    11761 taaaaggcag caagtatgta ttggaaaata aagaagtaga atatccgaca tcttatgcaa
    11821 gtaacgaatt tctttcagat accgcagaat tttctttttc agatggtatg attgcagtta
    11881 ttcaaagcaa agattgatcc ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga
    11941 cagaatctat tatttgctaa aaaaaagctt aagaggagca acttaccttt tgtttcacgg
    12001 catttagtga taaaattgaa cacaagagtc taattaattc gaggtgtctt tgtgaagaag
    12061 ttcaatccta ataaaaatat tattattacc ttggttattg ttatcatcgt ggtgacgata
    12121 ctaagtgtga ccgctgcgca gcgagcgaat gaaggaaagc ccaatttctt ccaatccatc
    12181 gttaatgaca gtgttgcaat ggttgataaa acaatctctg ccccagtacg gtgggtacga
    12241 aatggagtag attctgtcca taatcttttt tcaacttatg ccgaaaatga gcggctaaaa
    12301 gaaaaaatcg atagctatga tgacgtttca cagcaaaaca agaattacga gagagaaatc
    12361 gaagcgttga aaaaagaact tgagttgaat cagacgctga ctagttacga aaaagtaacg
    12421 gcaaatgtca ttactcgttc gcctgacagt tggcaggaca tgctgatcgt agacaagggt
    12481 tctaaagacg gtatcgaagc aaatatggca gtcatgtcgc aaaaaggatt gattggccga
    12541 gtgatcgagg tcaatacggc ttcgtctaaa atcgaattac tgtcatcctc taatgaaagc
    12601 tccaatcatt ttccagtacg ggtatcttcg gctaatggcg aagcgtttgg tttgcttaaa
    12661 aactatgatg aaaagctcca tgccttagtg gtgacccaat taactggtga tacggatatc
    12721 aaagaagggg atgttgtcca gacatccggt cttggaggga attctccagc taacttgccg
    12781 atcggtacgg ttattaaaac gaaaccagat agttatgggc tggatcggga agtttatgtg
    12841 aaaccttatg cagaaatgta tgacgtgtca gttgtgacga ttgtccaacg gctagcggag
    12901 gattagtcca tgttgaagaa agaaacaatg aagtactatc tgccaatcgt tttgttcttt
    12961 ttgatgttga tagatggtca tttaacaaga atgctagggg agtggtcgaa aggcacctat
    13021 atgtcaaatg cccactttct gatattggca ttattatgtt gcagtatggc gtttgaaaaa
    13081 cgttatttac tgattaccac gattgttctc ggggctatct atgacgctta ctatattggc
    13141 gttatcggta tctatgcagt agctctccct ttaattgtat ggttgatgta tgtaatgaaa
    13201 gacgttatcc atgtcaacat ctttactgaa tttttcagta tgatcatctt tgtcacgggt
    13261 tatgaattgt ttacgatggt ggtccagttg atttttaaat tagcagtagt aaataacacg
    13321 tattttatta caaggttttt aggacctaca ctgctgttga acatgattat atttgtatta
    13381 ttcatttttc cctttaagaa attattcagt gatgaataat ttcttttttt atttttaggt
    13441 cgaaagcgaa aaagaatcgt tgttatctta agaaatgcta gacttaacgc ggctttttca
    13501 tgatcatttt ctgaaacgaa cagttttgtc atttttaaaa gttgttttga aacatttttg
    13561 taacataact attatatcgt tgacacactg ctatggtaca atttttatat cgttaagaaa
    13621 gacttaaata tatttagata tttattaaat taagccagtc ggaggaatga agagtgaaaa
    13681 agagtttaat atcagcagta atggtatgtt caatgacctt gactgccgta gcatcaccga
    13741 tcgctgccgc ggcggacgat tttgattctc agatacaaca gcaagatcaa aagattgcag
    13801 acttgaaaaa tcaacaagca gatgcacaat cacaaatcga tgccttagaa agccaagttt
    13861 ctgaaatcaa cacacaagca caagacctat tagcaaaaca agatacattg cgtcaagaat
    13921 ctgcacaatt agtaaaagac attgcagatt tacaagaacg tattgaaaaa cgtgaagata
    13981 caatccaaaa acaagcacgc gaagcacaag taagcaacac aagctcaaac tacatcgatg
    14041 ctgtattgaa tgctgattca ttagcagatg ctattggacg cgttcaagca atgacaacga
    14101 tggtaaaagc aaacaacgac ttgatggaac aacaaaaaca agataaaaaa gctgtagaag
    14161 acaaaaaagc tgaaaacgat gctaaactaa aagaattagc tgaaaaccaa gctgctttgg
    14221 aatctcaaaa aggcgatctt ttatctaaac aagctgattt gaacgtactg aaaacttcat
    14281 tagctgctga acaagcaact gctgaagaca agaaagctga cttgaaccgt caaaaagcag
    14341 aagctgaagc agagcaagct cgtattcgtg aacaacaacg tctagcagaa caagctcgtc
    14401 aacaagctgc acaagaaaaa gctgaaaaag aagcacgcga acaagcagag gctgaagcac
    14461 aagctactca agcttcatct actgcacaaa gctcagcaac agaagaatca tcagctacac
    14521 aatcttcaat gactgaagaa tcatcatcag ctacacaatc ttcagcaaca gaagaatcaa
    14581 caacacctga atcttcaaca gaagaatcaa cagcgcctga atcttcagca acagaagaat
    14641 caacaacagt gcctgaatct tcagcaacag aagaatcaac aacagtgcct gaatcttcaa
    14701 caacagaaga atcaacaaca cctgcgccta caacaccatc aacagatcaa agtgttgata
    14761 caggaaacgg cacaggaagt agtactccgg ctccaacgcc aacaccaaca cctgaacaac
    14821 caaaacctgt aacacctgct ccagctcctt ctggaagtgt gaatggtgca gcaatcgtag
    14881 cagaagctta caaatatatt ggtactccat atgtttgggg cggtaaagat ccaagtggct
    14941 ttgactgctc agggttcaca cgctatgttt acatgcaagt aactggtcgt gacatcggtg
    15001 gttggacagt tcctcaagaa tcagctggaa caaaaatttc agtttctcaa gcaaaagctg
    15061 gagacttgtt attctggggg tcacaaggcg gaacttacca cgtagcgatt gctttaggcg
    15121 gaggacaata tatccatgct cctcaaccag gcgaaagcgt aaaagttggt tcagttcaat
    15181 ggtttgcccc tgactttgct gtcagcatgt aagaatgatt aatcaaaaag agttagccca
    15241 ttctggacta actctttttt tattgtgata aaatagaaat cagataaatt cttctgtcaa
    15301 ttccatgaac gtttggatat cttcagtcac catgtcgata cctgcttgcc aaaaatctgg
    15361 ttgggtaaga tcgacattca aatgtttctt cgcaagatct tccgtcgtca ttgaggcagt
    15421 atcgcgtagt aaatggatat attcttcttc aaaatttgct cccttttggt tggcgtaagc
    15481 atagatgccc aaactaaaga ggtagccaaa tgtgtatggg aagttataaa acggtacgtc
    15541 atcaataaag aaatgcaatt ttgcagccca gaaatatgga tggtaagtag ctaaagctcc
    15601 ttgatagcct tctttttgcg cctcaaccat catctctgtg atcttatctt ctgagactaa
    15661 tccttcttgc cgtgcttcat agaatcgatt ttcaaaaatg aatcgggaat ggatattcat
    15721 gaacatagct aaagcatttt gcagttttgt atctagcaaa ttgatttttt cttcttttgt
    15781 ttttgcgact tttaatgtag catcggctac gatcagttct gcgaatgtac ttgctgtttc
    15841 tgcgacattc atggcatatt cgcgatttaa agaaggtaaa tcccacatcg tgctgctatg
    15901 gaaagcatgt cctagttcat gagcaagtgt cgcaacctca ttaacagaat tactgtaggt
    15961 cataaagatc cttgattctt ttgtttctgg taactcagta caataaccac caggacgttt
    16021 cccaggacga tcttctgctt cgatccagct cttttcaaaa gcagattggg caaatttagc
    16081 catttttgga ctgaatttgt taaagttttc gataataaat gctgctgctt cgtcaaaact
    16141 aaaggttttt tctttcaaat cgcctaaaat aataggtgca tcttgatctt gccattccat
    16201 tttttcttta ccgaaaagat tagcttttct agtcaagtaa ttaacaaggg gttgtttgtt
    16261 tttctggatt gtatcccaca tgacagacaa tgtttctttc ttcaaccgat tgtactctaa
    16321 tggtttttgt agaaaatctg tgacaccatg aagttcatag tcagaaagtc taaaaccatc
    16381 taaatgattc aaagtatccg tgaagagggg tgccttttct ttccaggctt cttcccatgc
    16441 tgcgaaaagt gtttctctga cttttggatc tggatcgccc atcatcttat tgaaggcttg
    16501 tccagcagat aattcgacta cttgtccatc ttgttcgaag ggaatcgaaa tgctggctac
    16561 aatcgtatca taatgactgc tccaagcatt tagaccatct aaagaaagcg tatttataat
    16621 gttttcttca gcttctgata ataattgtga gccatcacga cgaatctcgt ttaaacgaaa
    16681 agcaattgtt tcaaacgaag attgagaaag catctgattc caatcattgt cagatatttc
    16741 tgcaaatttc tttgacaata cagtttcggc tgattgcaga cgaggcagca ttgcgtaaag
    16801 cttgcctgat aagacctttg catctgaatc attgacattg gcagaaagca atgcagtaat
    16861 atagctgctg cattgggtaa aaccattcgt aatcgtttct tgcaattgaa gaatagcgtt
    16921 taactgctct gcattgtcag aactaggtga ccattttgtt acgcggtgat aatactcgtt
    16981 catttggtct tccaattgct tcatccgttg gttcaaagca tcagaatgac tgcctccgga
    17041 aaaaatagaa tctagattcc aatttaatga ataactcatg aaaaaactcc cttcatattg
    17101 taatacaaaa ttaagtatag cactttttta tggaagaagt ctctatgaaa atgattgttg
    17161 tttcttcaaa aaaagtctaa gataaaatac agaggtgact tatgaattct tcaaaaaaac
    17221 aaatgaaaaa cggaaatatt tatttagtaa tcgcactgat agtcgttata atcttatttt
    17281 acagttcttc tcaaacttac ggacagcaat cacaagtttc gcgattagaa agctggctgc
    17341 ctaatcagcc atttaaagac acattagcgg gtatacgttt taattatggt ggaagtatgg
    17401 tgagtatcga ccatctgggc tacgccaaat ttttagagtt ctttttaagg aagggcgcac
    17461 atttcggtac ctattttatt ttgggaggca gcttgttttt aggtgttttc cccaagctaa
    17521 aaatttggtg gctgacagcg atccttgctt ggctttcagc aacagggtac gcaggtttgg
    17581 acgaattcca tcagatgatg accggtggac gaacacctat gttccaagat gtcatgttgg
    17641 attcgatcgg tgctctgact gctgtgatat tatgtgtgct ttttgcggca ttcaagaagc
    17701 gtttatagat tggctgtggc tttgtttttt tgtgaaatag tactagaata gaagtgtatt
    17761 ctcaaaagaa caagggagga aaaatcatgt caggacatag taaatggcat aatatccaag
    17821 gaagaaaaaa tgcccaagat gcaaaacgag ggaaagtctt tcagaaactt tctcgtgaaa
    17881 tttatatggc agctaaagca ggcggtcctg atccatctat gaatccagct ttgcggcttg
    17941 ttgtggacaa agcaaaagct gcaaatatgc ctaacgacaa tgtggaacgg gcgattaaaa
    18001 aggctacgag tgccgtagat gaaacgaatt acgatgaaat cacgtatgaa ggatatggtc
    18061 cgggtggagt aggaatctta gtccatgcat tgacagataa ccgtaatcgg acagcgacaa
    18121 atgtccgagt agcttttact cgaaacggtg gatcactggg agaaacgggt tcagtcagtt
    18181 acttgtttga ccgcaaagga tatatcgcta ttgaacgcga tgggttgact gttgatgaag
    18241 agacgatgtt tgaggatgta ttagaggcgg gggcagaaga tctgcaaact tctcctgaag
    18301 tattcgagat ttatactgca ccagaagatt ttgctgctgt acgagatgag ttggaaaaag
    18361 agttcacttt ggcgcaagca gaattgacga tgattccaca aacgacgatt gatttaaatg
    18421 aggaacaaaa agagcaattg gaacgtctag tagataagct agaagacgat gatgatgttt
    18481 cagaagtctt tactgctgct gaaatgtaag tagcacattg ttagataata aaaagaaagc
    18541 acctgtacca atcgtacggg tgctttcttt ttatatatta tttttgttag aagaccaata
    18601 caggcgttcc tttttcgacc atcccgaata attccttcat tacacttggt ggggtgttga
    18661 tgcagccgtg tgaaccgcgg gttttccata agtctccacc gtattcaggt tgccagtctg
    18721 aatcgtggat accaacacct gtccagtcaa tcggcatcca gtagtttacc ggactctcgt
    18781 aaggtgttcc atcgtcattt gttcctttta aagttgcatc ttcttcttta ttccaaacat
    18841 agaagactcc agcaggtgta ggtgtagttg gctttccgga aacgatatct gtttcaaggg
    18901 ccacttttcc gtctttgtaa taccacatat gctggttctc cagatcaact tcgatatacg
    18961 tatcttcgat caatggatga tcagcggttg tgccaccttg cacaatgggt gaacgagtaa
    19021 aatcttggcc tgccagaatc gcttttttca atgcttctgt ctcactgtct gtttggatcg
    19081 tccaactgta agtgccaact gggaccgtaa cttctcctct ttttgtactt ttaaattttg
    19141 tgtcattcgt acttgtgtta tactttgtgc ctaaatctgt tacatattga cgtacttgtt
    19201 ctgtatctaa atcgactttt ccatcattgt aggtcagcca actcataata tcacttgatg
    19261 ggatctggaa agtctcaccg tttatactgt aagtagcttt tacatttgcg atagcattca
    19321 tggatgcgag ttgttctttc agtgagctgt cttcagatgt tacttttggt ttttctttga
    19381 attcagtcaa ttcaattgta tctttaccag agtttaccgc actttttaat tgtttgaccg
    19441 cagcatcgac gtcgatcgta tttccattga cttcaggctt gataacgaaa gagtcacctg
    19501 ctttttcgat cgttgcatca gtagtaggtg tccggtcttt gttcaaatca gtcagcttag
    19561 ttgtcaatgt ctgaacggta gtgtctagtt tttggtggtt ttgactgaca ggatctattt
    19621 cttgtttttc tgccgctgag acataagtca ttccccaagt ccatccgttt tgttcattta
    19681 ggatcggaga aagctccgaa gtgaaatcat ctttgtagcc gagctcggat tttggtactt
    19741 cctgccagac ttttccatct tctttaatag ccagtttttt atcagaataa gcttctttta
    19801 atttttcatt tgctttttcg actgttagat tgctgacatt tattccattt atttctgtat
    19861 tcggcaaaaa atgctttgtg tagtatgtac tgcgataggt ataaaagctg atcagcacta
    19921 agagaacacc aagaactgaa agcaaagcaa catgtttcga tttttttcga tgcgaagatc
    19981 ttgtcatatc gacaactcct taagtaaaag tttagggtgg aacggtctca tgatagcata
    20041 atttagagga aaagacgaag gttatctcta ctttatgaaa aaatttagaa accataaggg
    20101 tgataaaata aaaaaacgga aaacaccgaa tagatgtttt ccgtcatgtt caaagattat
    20161 tcagcgtcta gtttagcaaa agcttcatct agaatttctt ttaattgctt agcagaagca
    20221 gccatacggt cttgctcgct gtcacttaaa ggaatttcga ttactttttg gataccttga
    20281 cggttgataa cagcaggtgc accaatgtag atgtcgttca atccatattc gccattcatg
    20341 aatactgata aaggaagaac agcattttca tcatctaaga tggcacgagt gatacgtgct
    20401 aatgctactg cgataccata gaaagtagcg ccttttttct cgataattgt gtaagcagcg
    20461 tcacgtacgc caaagaatag gttaaccata gcttcttcat cgatttctgg gttgtttttt
    20521 acccattcat agatttgcaa gccggcaaca tttgcatgtg accaaactgg gaattctgaa
    20581 tctccgtgtt cacctaagat gtaagcatgg acgttacgtg catcaacgtc aactaattcc
    20641 gcaagtgctt gacggaaacg agctgagtca agagaggtac ctgaaccgat tacgcgttct
    20701 ttagggaaac ctgagaattt ccaagttgag taagtcaaaa tatctactgg gttagcggca
    20761 actaaaaaga taccgttaaa tcctgattca acgatttgtg tgacaacatc gcggttgatt
    20821 tttaggtttt tgtttactaa gtctaaacga gtttcacctg gtttttgtgg tgcgccagct
    20881 gtaataacaa ctaagtcagc atcatgtgcg tctgcgtaag tagcagaata gattttttta
    20941 ggtgaagtaa atgccaaagc gtgagacaag tcaattgcgt ctccttcagt tttagcagta
    21001 ttgatatcca caatacctac ttcttgagcg atattttgag taactagagc aaaggcatag
    21061 ctagaaccta cggcgccgtc tccaacaaga ataacttttt gatgatcttt tttttctgca
    21121 gctgcagtca tttagtatca atccttccat tgatttgttt tttcccatgc ttatgctaac
    21181 atttctcaag ccccatgtca cgctatttgg ctataaaata taaca
//
LOCUS       DAGCYL010000028        20970 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.028, whole genome shotgun sequence.
ACCESSION   DAGCYL010000028 DAGCYL010000000
VERSION     DAGCYL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.028"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            606..1295
                     /locus_tag="MB216_000830"
     CDS             606..1295
                     /locus_tag="MB216_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4525688.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1282..2490
                     /locus_tag="MB216_000831"
     CDS             1282..2490
                     /locus_tag="MB216_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4525689.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2784..4055
                     /gene="serS"
                     /locus_tag="MB216_000832"
     CDS             2784..4055
                     /gene="serS"
                     /locus_tag="MB216_000832"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBT4525690.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4604..6088)
                     /gene="guaB"
                     /locus_tag="MB216_000833"
     CDS             complement(4604..6088)
                     /gene="guaB"
                     /locus_tag="MB216_000833"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBT4525691.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6593..7288)
                     /locus_tag="MB216_000834"
     CDS             complement(6593..7288)
                     /locus_tag="MB216_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBT4525692.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7378..8478)
                     /gene="ychF"
                     /locus_tag="MB216_000835"
     CDS             complement(7378..8478)
                     /gene="ychF"
                     /locus_tag="MB216_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBT4525693.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8487..8678)
                     /locus_tag="MB216_000836"
     CDS             complement(8487..8678)
                     /locus_tag="MB216_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBT4525694.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8699..9589)
                     /locus_tag="MB216_000837"
     CDS             complement(8699..9589)
                     /locus_tag="MB216_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBT4525695.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9576..10343)
                     /locus_tag="MB216_000838"
     CDS             complement(9576..10343)
                     /locus_tag="MB216_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBT4525696.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10355..11068)
                     /gene="rsmG"
                     /locus_tag="MB216_000839"
     CDS             complement(10355..11068)
                     /gene="rsmG"
                     /locus_tag="MB216_000839"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBT4525697.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11300..13201)
                     /gene="mnmG"
                     /locus_tag="MB216_000840"
     CDS             complement(11300..13201)
                     /gene="mnmG"
                     /locus_tag="MB216_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBT4525698.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13218..14615)
                     /gene="mnmE"
                     /locus_tag="MB216_000841"
     CDS             complement(13218..14615)
                     /gene="mnmE"
                     /locus_tag="MB216_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBT4525699.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14914..15297
                     /locus_tag="MB216_000842"
     CDS             14914..15297
                     /locus_tag="MB216_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525700.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15465..16223)
                     /locus_tag="MB216_000843"
     CDS             complement(15465..16223)
                     /locus_tag="MB216_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBT4525701.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16241..17059)
                     /locus_tag="MB216_000844"
     CDS             complement(16241..17059)
                     /locus_tag="MB216_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBT4525702.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17056..17412)
                     /gene="rnpA"
                     /locus_tag="MB216_000845"
     CDS             complement(17056..17412)
                     /gene="rnpA"
                     /locus_tag="MB216_000845"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBT4525703.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17530..17664)
                     /gene="rpmH"
                     /locus_tag="MB216_000846"
     CDS             complement(17530..17664)
                     /gene="rpmH"
                     /locus_tag="MB216_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBT4525704.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18187..19521
                     /gene="dnaA"
                     /locus_tag="MB216_000847"
     CDS             18187..19521
                     /gene="dnaA"
                     /locus_tag="MB216_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBT4525705.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19719..20849
                     /gene="dnaN"
                     /locus_tag="MB216_000848"
     CDS             19719..20849
                     /gene="dnaN"
                     /locus_tag="MB216_000848"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBT4525706.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5995 a         4267 c         3839 g         6869 t
ORIGIN      
        1 aggtctactt catcaggagt gtttcccgat tcgtttttgg ctgccgtagc aacttttata
       61 tcatatcaga gagacaagta attgtcaact cttttttaaa agttttttcg atttcgtttt
      121 tcgaaatctt ccagcaactc atttatcata acttatcgtt tgttatttgt caagaacttt
      181 tttgaagttt ttttcgatag aatcaagctt cgcaagtggt tcttttttaa cgactcagtt
      241 atcttatcat gtcatttcca acatgtcaac aacttatttt ttattattta ataagttatt
      301 ggtgttcttt tgaacaaata ataatatatc aacatcacat gtgaaagtca ataagaaaat
      361 gcttatttat caacatttct agcttacatg atcattggtt agttctggtc ctttttcgtt
      421 cgtctttata actccgagag acgtaaaatc cttttgtttt cagtttaatg ttcatgttct
      481 tgtcttattc acaccaacta gatgtctcac cttctaaaat tttccttaac aaagtgctgc
      541 ttttatctat ttctgctttt ttttgctaga ataatagtaa caatatgact ctggaggatt
      601 tatttatgaa aattttgatt gctgacgatg ataaagaaat cgttgaatta ttaagtatct
      661 atgttcacaa tgaggggtat gaagcagtaa aggcttatga tgggaaagaa gcattatcta
      721 aaatcagaac aatccctgat attgaattac ttattttaga tatcatgatg cctgagatgg
      781 atgggatgca agtagtgaaa gagctacgaa aagaatccca aataccaatc attatgctga
      841 cagcaaaaac tactgatatg gataagatca aaggactggt cgctggtgca gatgattatg
      901 tgaccaagcc atttaatccg ttagaagtaa tggctagagt aaagtcttta ttacggcgta
      961 caaaaatgca gattgcccca gatcagcctg atgtattaga agtaaatgca ttaatcatca
     1021 ataaagactc tcatgaagtt aaaacaatcg atgggaaaga aatccagtta acagctttgg
     1081 aattcggtat cctctacttg cttgctagtc atcctaatcg agtatttagc gcggatgaaa
     1141 tttttgagcg tgtttggaaa caagaaagta ttgtctctgc taaaactgtc atggttcacg
     1201 ttagtcattt aagagataag attgaagaag caactggtgg tgaaaaagtc attcaaaccg
     1261 tttggggagt gggctataaa attgacgcaa gatagtaaac gagcaactga aaaacgccgt
     1321 cggatcaatt tgacttcaaa agaaatcagc gaattgttag cggaaggaat catcacgatt
     1381 atccttttgc ttctattaaa tgtttctatt ctggttgtta tcagttcggt gatcaacagt
     1441 tcaccttctt tgacgaatgc gatttgggat tcaaagaata tttttgcaga acgattgaac
     1501 actgatttgt tctggaacgg ccggaatttt attattccct ttttctttct attagatatt
     1561 ggtgttttgt attggcgctt gattcgaaga tatcgccaaa tgcaattgcg gcatatcatc
     1621 agcgaacttc actacatcgc aaacggaaat tatgatcatc gtatcccttt tgaactgagt
     1681 ggtgacttaa gccgagtggt aacaagtatc aatggattgg tggatagtac cgtagcagcg
     1741 attgaagacg aacgtaaaat cgaaaaatca aaagatgaat tgatcacgaa tgtcagtcat
     1801 gatatccgta ccccactcac gtccatcatt ggatacctag gactgataga agatggccag
     1861 tatcactcag aagaagatct tttaaagtat acgcatactg cttatatcaa agcaaagcag
     1921 atgaaatctt tagtggacga cttatttgaa tacactaagg tacgccagcc tgctgtcccg
     1981 gtgaattttt ctgcctttga catgatccag ttgatcgaac agttagcagc tgattttgag
     2041 ttggaagctt ctaaaaaaaa tatccaaata cttgttcaat caaaagtaga ctcccttatc
     2101 atggatggcg ataccgaaaa acttgtacgt gtcttcaata atcttttgac gaatgcgttg
     2161 aaatacggaa aaggagcaac gaagatcgta atcgaagtag aacgtatcgg ttcagaagtt
     2221 gttgcaacag tcaaaaataa cggagcaatg atcccacaac aggcaatcga taatctattc
     2281 gaccgttttt atcgtgtaga agaatctcgt tcacaagcaa ctggaggaac tggacttggt
     2341 ttagcaatcg ctcaaagcat tgtggctttg cacggcggat acatttatgc aaaatcagat
     2401 aagcaatgga cttctttcat catgcacctt ccaatcaaga aaaatgaaaa attacctatt
     2461 gatacacagg aagttattga cgaaagctga aatattcgct attcttatta caattattag
     2521 aataaatgaa aaacacagaa cccagtacac tttattcttt ttcagagagc tgatggcagg
     2581 tgtaaatcag caaaggataa tttggaagtt ccagttcgtt tacgtgccct ttcgaaataa
     2641 gaaaggtcgg ttcaacagcc gttatgtgtt caacaagtgc agctgacgct gaacttgggt
     2701 ggtaccgcga atttcagact gattcgtccc aagaataatg ggatggatca gtcttttttt
     2761 attttcaaaa aggaggaaat catatgttag atgtaaaaat gatgcgacaa aatattgaag
     2821 aagtacaaga aaaattgatg actcggggcg taaaaaaaga aacattagct gaattcatgg
     2881 aactagatga aaatcgtcgt aagctactag tcaaaagtga agaattaaaa aaatatcgaa
     2941 atgatgtttc tgctgagatc gcccaattga agcgtgcaaa agaagatgct tctgctaaaa
     3001 tcgctgaaat gaaagaagtc ggtggcaaca tcaaagcatt ggatactgaa attgctgaag
     3061 tggatgaaaa attgaaacaa attgcaacta ctttgccaaa ccttccccat gattctgtgc
     3121 cagttggaac agatgaagag gacaatgtag aaatccgtcg ttggagcgaa ccacgtacat
     3181 ttgcttttga accaaaacca cactgggaaa ttgcagaaaa tcttgatatt ttagattttg
     3241 aacgtggcgc aaaagtagcc ggtagccgtt ttgtctatta caaaggatta ggtgctcgat
     3301 tagaacgtgc tgtctacaac ttcatgcttg atcagcatat ttacgaacac ggctatactg
     3361 aaatgatcac tccttatatc gtcaacagta aagcgatgtt tggtacggga caatttccaa
     3421 aattcaaaga agatgttttc caattggaga atactgatct aacattaatc ccaacagctg
     3481 aagtaccatt aactaactat tataatgatg aaattttaga tggtgcagat ttacctattt
     3541 atttcactgc actaagccct gcttttcgtt ctgaagccgg aagtgctggt cgggatacac
     3601 gaggactgat tcgtcttcac caattcaata aagtagaaat ggtcaaattt tcggatgtag
     3661 agcattctta cgaagaattg gaaaaaatga cagctgatgc agaagatatc ttgaaaaaat
     3721 tgcatttgcc ttatcgtgtg atggccttat caactggaga catgggcttt tctgcagcta
     3781 aaacttatga cttagaagta tggatacctg cacaagatac ttatcgtgaa atcagttctt
     3841 gttctaactg tgaagatttc caagcacgcc gcgcaatgat ccgttatcgc gataatgatg
     3901 gaaaaatcca ctatactcat actctgaatg gttcaggact tgcagttggc cgtacagtag
     3961 ctgccatttt ggaaaactac caaaatgaag atggttctgt cacagttcca gaagcgctcg
     4021 ttccttatat ggggaatctg aaagttatta aataaagagg ctgtgaaaaa gccgcttagt
     4081 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaattggact
     4141 taataccgag aaactggctt ttgaacacca tttatcgaga ggttgtgaaa tcagcgaatt
     4201 aacctgagta ttagagaaaa aaatggaaaa atagtccctc atattttacc gtttgttgaa
     4261 ctaataccga gggtcagctg attgaacacc gtttagtgga gaggtcgtaa gcctaccccc
     4321 cagctcctag ttacaaggac aaattcaaaa aacagctctt catattttat gaatgttgta
     4381 cttgtgattc ggaggtggca agggaacact gtttattcaa aaagaggctg ggacagaagc
     4441 gtttaactcc aagaaataag aagaaattca cgaaaattgc ttttcaaatt tttgtgaatt
     4501 tcttcttatt tccgaaggag ttgcttctac ttccgccgtt tatacgtgtt tagagcgtga
     4561 gacaaaagtg ttctttactt ttgtcccacg ctctttaatg tttttaagat tcaactgaat
     4621 agtttggtgc ttcttttgtg atttgtacat catgagggtg tgattctttt aatccactgc
     4681 cgctcatttg gataaattgc gcttcatcac gtaattgctg tagattggct gccccaacat
     4741 aacccattcc tgaacgcaaa ccgccgatca tttggaaaat aatatctgca acacttcctt
     4801 tataagcaac ccgaccttcg attccttctg gtactaattt atttgcttca ttgacactac
     4861 cttggaagta acggtctttt gaaccttttt ccatcgcgcc taatgagccc atacctcggt
     4921 atgttttgaa tcgacgtcct tggaagattt cgaattcacc tggtgattca tctgtccctg
     4981 ccaacatact tcctagcatc actgcatgtc cgccggcagc taatgctttg acgatgtcac
     5041 cagaatattt gatccctccg tctgcaataa tcgctttgcc atattgacga gcaactgatg
     5101 ctgcatcata aatagctgtg agctgaggta cacctacacc ggctaccaca cgtgtcgtac
     5161 aaatcgatcc tggtccgata ccaactttta cgacatctac acctgcatca tataaagctt
     5221 ttgtcgcttc agccgtcgca acgtttcctg caatcaatgt tgcatctgca aaagttgaac
     5281 ggatttcttg aatttttcgg atcacacctg cactatgtcc atgagctgta tcgatgatga
     5341 ttgcatctgc ccctgcttct aataaagcat tcgcacgctc aaatgtatcg cttgttacac
     5401 caacggctgc tgctacaagc aatcgaccat gttcatcttt tgctgcattt ggaaattcga
     5461 tcactttctc gatgtctttg atcgtgatca atccgcttaa acgaccttcg ttatctacga
     5521 ttggtaattt ttcgatttta tgtttttgca aaatcttttc tgcatctttt agagaggtac
     5581 ctactggggc agtcactaag ttgtcttttg tcatcacatc agcaatcggc atgtgataat
     5641 ctgttacaaa acgcatgtcg cggtttgtaa ttatccctac caattttctg ttttcaagtg
     5701 tttcaacgat tggcacacca ctgatacgat atttgctcat caattcttct gcgtctgcta
     5761 caagatgctg tggcgttaag aagaacggat caatgattac gccactttca gaacgtttga
     5821 cttttcttac ttcatccgct tgctggctga tcgtcatgtt tttatggatc acacctagtc
     5881 cgccttgtct tgccatagca atggccatct tgctgtcggt aacagtatcc atacttgcac
     5941 tgatgattgg aatattcaac ttaatatttt tagctaattg tacgctcata tccacttcgt
     6001 ttggtaacac gtgactttct gctggtatca ataagacatc atcaaaagta aatccttttt
     6061 tcgtgaactt tgtttcccag ttggacattt ttagtaccac tcctctaatt tagtttatct
     6121 attaaaataa ttgatttttt catttaagtc aatgatctac ttggaatcaa gttagctttc
     6181 tcatagaata ccaatataaa gcgtttacat ctgtgtatat taaaagagat tgtaaaaaag
     6241 ctgtcctgca tcaagtaata agaacagcca atcaaaaaca gcttctcatg tttttgaatt
     6301 ggttgggttt attaccgcag atgcaaactt ttgaacaccg tttagtggag aggctgtgaa
     6361 aaagctgctt agtttcgagt agtaagaaaa attttgtgaa aatggctttc cacatttttg
     6421 cataaattgg acttaatacc gagaaactgg cttttgaaca ccgtttatcg taaaaaggga
     6481 ctgtgacaag acttttgtca cagtccctta ctattattta actatggata aacaaaatga
     6541 ggttctttac tacccctaga tatctagcaa aatagctgat ctttttgcta gtttatcttc
     6601 ttgggacgga taaacttcca ataatcccgt attttttctt caagtagtaa cggattgcta
     6661 aagcagttgc cccgataatg ataactacta ctggatctaa tactggattg ataactggtg
     6721 gcaatagtgt tgtcgcagaa aacagtacga cccaaagaag tgtcgttcca cctaggataa
     6781 gcaatgtctt acccattcct ggtttcttgc ttcgatctgc tccgggatat tcatattgat
     6841 agatgtattt gtacatcata taaaagaccc agccaccgat tgcagaacct agaacgagtg
     6901 tagaaaagcc ataagctggc gttgttccac gtgaaaacaa tccggtaatc gcaatcatga
     6961 tacttaatag accaagcaac agcaacgtat tatctaacca catcaataaa ggggtggatg
     7021 aattttttac ttctgctggc ttattgatga ttgcttccgt tctttcagag actgtcccaa
     7081 acaattgacg ggctgttctt ccgccttttt gttctgcaac taaagtaggc agaatgtcat
     7141 gaagggcttt tactttttct tcttcagaca aattcgctgc ttctaaagat tttttcaaat
     7201 caaaaatgta ctgttgattt cttttggtta attttgtttc taaactattg ttttctttta
     7261 cgattgctcg taattgttct ggttccatta tctaatagtc ctccttagcc gttccttttc
     7321 tcaacggtcg tcacaaaaga cattctacca tattctacag ctagtttgat agccctttta
     7381 cacattaaag cggaataaca ttacgtcgcc gtcttgtacg acataatctt ttccttctaa
     7441 tcgaacacgt ccagcttctt ttgctgcatg catgtttccg taatgatcca agtcttcaaa
     7501 agagacagtt tctgcacgga taaatccgcg ttcgaaatct gaatggatga ttcctgctgc
     7561 ttgtggtgct ttgattcctt ttttgaatgt ccaagcacgt acttcttgtt ccccagcagt
     7621 aaagtaagta gctaagccta acaaatcata agcagcacgg atcaactgat ccaaacctga
     7681 ttcttcgatt cctaaagctt ctaaaaattc cgctttgtct tcttcatcta attctgcgat
     7741 ttcttcttct gcacgagcac tgaccacgat cacttctgca ttttcttctg cagcaaagtt
     7801 gcgcacttct tgtacataat gattcgcgtc tgcatctgct acgtcatctt ctgagacatt
     7861 ggctacataa agaattggtt ttgtcgtcaa taaaaatagt gatttaacga ttttttgttc
     7921 ttcttcagta aattcgatcg ttcgggcaga ttttccttct tccagtacag gtttgatttt
     7981 atccagtaca gctagttctg ccactgcatc tttatctttg gttttagcaa cttttgctac
     8041 acgagtatag cgtttgttga tagattctaa gtctgctaac actagttcca agttgatcgt
     8101 atcaatatct tctagtggat caacacgtcc ttctacgtga gtaatattat catcatcaaa
     8161 acagcgaaca acgtgacaaa tagcgtctac ttggcggata tggcttaaga attgatttcc
     8221 caaaccttct cctttacttg cgccttttac gattccagca atatctgtaa attcaaaagt
     8281 cgtcggaaca gtttttttcg gatgtacaag ttctgttaag cgttgcaaac gccagtcagg
     8341 tacttctacc atccctacgt tggggtcgat cgtcgcaaag ggatagtttg ctgcttctgc
     8401 tcctgctttt gtgatcgcat taaacaaagt tgattttcct acgttgggca acccaacaat
     8461 tccagcagtc aatgccataa atttattcac tctttctctg catttttttc aattactttt
     8521 tttaatttct tttcaaaatc tcgacgggtc atcatgacga tatgtccgca attcatacat
     8581 ctgattttga tatctgcgcc cattcgaatg acttcccagc gattcgtctg gcaagcgtga
     8641 ggttttttca tttcaacgat atcgtgcaag tcgtacataa aaatagcctc ctttttgttt
     8701 attcttcatc aaattgaata ttcaagatat caagaattcg agtcaaatct ttttgagaaa
     8761 gatattcaat ctcgatctta cctttcccat ttttttcttg gatagctaca cttgtaccaa
     8821 atttatccat caaacgatct tcgctttctc ggatataata tggtttttcc tttgctagcc
     8881 ttgggatttt tttcttttct tttccatcat tttgatttaa ctcagaaaca agctgttcca
     8941 gttggcggac agttaaattt tcagttactg cacgattggc taatttcaag atcaatgttt
     9001 tattttttaa acctaacagc gtacgggctt gtcccataga taaacgctga tcttgtacca
     9061 tttccttgac aagatccggt aaagaaagca agcgcaaata attggcgata taaggtctgc
     9121 tctttcctaa acgactagct acttctgctt gtgttagttt caggtttttc atcagcatct
     9181 catatgcttc tgcttcttct aacggattca gatcttctcg ctgcaagttt tccagcactg
     9241 ctacttgcat catcgcttct tcgtcaaagt ctctaacgat agcagggatc gtttcttttt
     9301 cagctagttt ggacgctcga aaacgtcgct cccctgcaat aatctcatat ccttttactg
     9361 cggaacggcg tacgatgatg ggttgaaaga cacctgattg ttcaatagaa ttagctagtt
     9421 cctgcaaaga tacttcttca aatgttttac ggggctgata cggatttggt cgcagctcat
     9481 tcaatggtag ctggacaacg gtatcatctt ttacatccac cgtctctaag ttttctaaat
     9541 cttgaaacag tgcatcgatt cctcggccta atcctttact tttcttcacg acttaccact
     9601 tcctttgcca atgcttggta gacttcagct cctcgagaac gtggatcgta gtcgatgatc
     9661 ggttttccat gactaggtgc ttctgataaa cgaatatttc ttggaatgat cgtttcgtaa
     9721 actttttcgc ggaagtattt acgtacttct tctacgactt catttcccag atttgttcgt
     9781 gcatcataca ttgtcagtaa gactccctcg atctctaatt ctggattaaa gtgtttttgg
     9841 actaaacgaa ccgtattcag taattggctt aatccttcca atgcgtaata ctcacactga
     9901 acaggtatca aaatcgaatc acttgctgta aatgaattga ttgtcagatg gccaagagaa
     9961 ggcgggcaat cgatcaaaat ataatcatac tgactgctta cttctgctaa agatcctttt
    10021 aatcttgatt ctcttgccat cattgacgtc aattcgatct ctgctcctgc taattggaga
    10081 gtcgcaggca caatactcaa attttcgtgt tcagtgatca aagttgcctc atcaattggc
    10141 aattcattga ctagaacatc atagatgtct cttgtcacat caggttttct gatcccgaca
    10201 ccactggtag catttccttg tgcatccata tccactagca atactttttt gccaaggcta
    10261 gcaagacaag cacctagatt caccgtcgtc gttgttttgc cgacgccacc tttttggttc
    10321 gcaacagata ttatttgtgc catctcttct cctcctattt gattggctgc ttattaggca
    10381 gtccgggctt tcttgggtat ttctttgggg ttggtttctt tttttgaata acgatgatat
    10441 gccgttgatc agagacattc ggcaaggtaa ccgcctcttc tttgaggaat ttaccaccta
    10501 ataaagcaat cgcttgtttt gcttctgtca attcctcttc actttttgct gctttcaatg
    10561 cataaaaata gccttcttgt ttagccaagg gcaaacacaa ttccgctaac acattcaaac
    10621 gagcaaccgc acgagcagtg acaaggtcaa atgatgcacg aaagtgagga ttttgcccaa
    10681 atgtttctgc ccggtcatga tataaggaag catcgattcc taataatcgg gttaactcag
    10741 ataagaaggt tattcgttta ttcagagaat caacgatcgt tatctgcagt tctggaaagg
    10801 caatttttaa aggtatgcta ggaaaccctg cccccgaacc aacatcgcat aaagataaag
    10861 gttgcgtaaa gtctacttca aatgctaatg taagtgaatc gtaaaaatgc ttcagatata
    10921 cgtctttttc atccgtaata gctgtaaggt tcatcttttc gttccattcg actaatgtcc
    10981 gataataaat agcgaattga ttcatttgat gatcggtaag ctctatccct ttttctgcca
    11041 aggattggcg aaattcttct ggtatcataa atattccttt cattgtgaaa cagcaattgt
    11101 ttcacagatt ctctccctac tttaacgcaa aatctctaga catgcaatcc ttccatatgg
    11161 attcttaaaa atttgaaaag taagaaaagc cgatagactt tttctttcta gtatgactca
    11221 taaaatatac acagaagaga gaactagctt tgagcaattt ctgcaaaaac tagttctctc
    11281 ttctatttat tattacgtat tacgctggtt cactgacttt cgcaatcttc ccttgttcga
    11341 tatagaccat taggatactt aggtctgcag ggtttacgcc actgattcgg ctagcttggg
    11401 caattgtttc tggttggatt ttctgcaatt tttgttttgc ttctgttgct aatccgttga
    11461 ttgctgcgta gtcgatgttt tctggaatct ttttcgcttc catgcgtttt aatttttcta
    11521 ctttttccat ggcttttttg atgtagcctt catatttgat ttggatttct acttgttcga
    11581 tttcttttcg tgtcaattct tcattttctg gaataaaccg tagaagttct gcataagtaa
    11641 tctcaggacg tcttaaaaag tcgcttgcta gtacaccatc ttttaatggt gccgcattaa
    11701 tcgttgctaa aaagtcttgg acttcttctg ttggtttgat tcgtacattc atcaaacgtt
    11761 tgatttcttg ttcaactgct tcttttttct tcaaataatt cttgtattga tcttctttta
    11821 ctaagcctac acggtgaccc atttctgtta atcgcaaatc tgcattatca tgacgtaaaa
    11881 gcagacggta ttcagcacgt gaagtcagca aacgataagg ttcgttcgtt ccttttgtta
    11941 ccaaatcatc gatcatgaca ccgatgtaac cgtcacttcg tttgatcact aaaggttctt
    12001 ttccctgaac tttcaaagct gcattgatac cggcaatcag cccttgtcca gccgcttctt
    12061 cataaccgct tgtaccattt gtttgtccag ctgtataaag attttcaacg actttagttt
    12121 ctaatgtcgg acggagctgg tgtggctcta ctacgtcata ttcgatagca tatcctgtac
    12181 gcatcatttc tactttttct aatccagcaa tagaatgaag catgttgact tgaacatcct
    12241 ctggtaaaga agtagataaa ccttgaacat agacttcttc cgtatttaag ccttctggtt
    12301 ccaaaaataa ttgatgacgt ggtttgtctg caaaacgcac gatcttatct tcgatggatg
    12361 gacaatagcg agcgcctacc ccttcaacga tccctgtaaa cattggggca cgatgtaagt
    12421 tgtctcgaat aatttggtga gtcgtctcac ctgtataggt cagccagcaa gattcctgat
    12481 ctagtttata ggcactatct ggtgtgctga agctaaaatg attcggctct ttgtcaccag
    12541 gctgctcttc tgtaacagaa tagtcgatcg tacttgattt tactcgaggc ggtgttcctg
    12601 ttttgaaccg ttcgatagca aagccaagtt cttttagatg attcgctagt ccaatagaag
    12661 gttgagagtt attcggacct gatgaatatt tcaattcacc gataatgatt tctccgcgaa
    12721 gtgctgttcc ggctgtaata acaactgctt tggcccggta gctcgctcct gtggacgtca
    12781 ctactccatg acaaacacca tcttcgacga tcagttcttc gacgattcct tgtcttagcg
    12841 tcaaatttgg ttctttttcg atcgtatgtt tcatttctgt tgaataagca tgtttgtctg
    12901 cttgtgctcg caatgctcgg acagctgggc cttttcctgt gttcaacata cgcatttgga
    12961 tataggtttt atcgatattc ttacccattt ctccgcctaa cgcatcgatt tctcgaacga
    13021 caacgccttt agctggtccg cctacagaag gattacatgg catgaatgct accatgtcca
    13081 aatttattgt cagtaataat gtttttgatc ccatacgtgc agcagcaagt gcagcttctg
    13141 aaccggcgtg tcctgcgcca acaacgatca cgtcatacgc atctgcttca taatgttcca
    13201 atcgtttgtt cctcctttta ttttcctaaa caaaattgac tgaataattg agtgattagc
    13261 tcatcttgta cactgtcgcc gacaacttct cctagatagt cccagcaacg cgtcatatct
    13321 atttgaacga gatccacagg cattcctgaa tcaatacctg caatgacttc ttcaagtgag
    13381 agggaagctt tttctaacaa cgcgatatgg cgggtattag aaacataagt agcgtctctt
    13441 tctcctgttt ctccgctgaa gaataaatca gaaatagctg attccaaacg atctagtccg
    13501 tcgtttttag ctacggaaat tgaaaaaact ggttcatttt cgatcaattt cttcaatttt
    13561 tcttgttcca gctgtgctgg caaatctgtt ttatttagaa gaatgatccg tttcaatcca
    13621 cttgtcgctt ccaataattg ttcatcttct gctgtcagtg gttcgctttg gtttagaaca
    13681 agtaagatca agtctgcttc tgctaatgct tttcggcttc tttcgacccc gatccgttcc
    13741 accacatctt ctgtttcccg aattccagct gtatcgatta gcttgagcgg taccccccga
    13801 acattgacat actcttcgat tacatctcga gtcgttcctg caatatccgt aacgattgct
    13861 ttttcttctc ttaacaaatg gtttaacaaa cttgatttgc cgacatttgg ccggccgatg
    13921 attgccgtac ttaacccttc ccgcagaacc ttcccttgct tggatgtagc aagtagcgct
    13981 tggattcttt gttggatcat ctgcgctttt tcaagcaata acttcgtagt caattcttcg
    14041 acatcatcat attctggata atcaatattt acttctactt gtgctaatgt ctctaaaatc
    14101 tcttgtctca atgagcggat caaagcagac agattgccgt ctaactgatt aagagctagt
    14161 cccattgctt tgtctgtttt tgcccgaatc aaatccatca cagcttctgc ctgtgaaagg
    14221 tcaacacgac cattcaagaa tgctcgtttt gtgaattcgc ctggctcagc taatcttgca
    14281 ccttctctta acaatagctg caaaatttga ttgactacaa cgatcccacc gtgacaattg
    14341 atttctacta catcttcccg cgtaaatgtc tttggtgctc tcatcacgga gaccatgact
    14401 tcatcgacaa gctgattgct cttgggatca acgatatgtc cataatggat cgtatgactt
    14461 ggtacttctg ataatcgttt gtttccagat tgataaactt tatctgctag ttgcacagct
    14521 tgatcgccac ttaaacgaac gatactgatt gcaccttctc caggtggggt agaaattgcc
    14581 gcaatcgtat caaattctaa agtaatttca gccatttttt cacttccttt ttcattttgc
    14641 gcacaaaaaa agtgcccact ccacccttta tgcacaataa acggtgaact tcgcactttg
    14701 actgcttgtt cttgattaaa ttctggtcat agattatcac acttgctata aggaatcaag
    14761 tgtttaaaac aattagatga agttgagcct gttctgcttc cattacctta tatttaattg
    14821 tccttggatg tctctttgcg ttctttggtt tattcgaaga gaagatactg tataaacaag
    14881 gctttatctt tcttaaaaaa aacggtacac taaatggtag cgctatctaa actatacact
    14941 tttgaaagga gaaaatatgt gagcccgttc agatctgctg cattggaaga agaaagtgac
    15001 cctcggatca acgatgtcgt tttatctaca gggaatatca accagaaata tatcgtccgc
    15061 gatatcgttt ttgttgtgga atgcttagaa gctgatttgt ttgacccatc ttttcccaaa
    15121 gatgaccttt tcttaacaac gaagaggaaa ttaaagacga aggtacttga atatggtgcg
    15181 aatgcagtaa tcaactgtca ttttgagtat cagcaaaaaa aacgcgacgg aaagtatttt
    15241 ctggaacttt ttgcttatgg aacagtgatc caatttatcc aaacaacgat tggttaaatg
    15301 aaacagacaa aaaaagcgga tcaaaactga agacagtttc gatccgcttt tttctatcaa
    15361 agttagtgac tacttcatcg tatactctga taagaatgat ttctagtaaa atatacttgg
    15421 tttgaaaaac tttgtcaacg tgatttattc aaaaagtaat ggaatcaata atatttttta
    15481 gcaggttcta ctactaaata acgataaggt tcctcacctt ccgaatgtgt ttttacatgt
    15541 tcatctttgc ttaatgcagc atggatttgt ttacgttcaa atgcaggcat tggttctaag
    15601 aaaacaggct gccctgtccg ttttactttt tcagccgtgc gatcagctaa acgctgaatg
    15661 atctcttggc gcttttcacg gtaattgcct acattgacta ctacagacag cttatttctt
    15721 gctaaacgat ggatgaagac ttgtgccaga tattgcaatg cattcagtgt ttttccatgt
    15781 tttccaatca gtatgccttg cttttgtgtt tctaaatgga aaacaaccat attatttgat
    15841 tctctttcca ttttgacaag agctggagca tcaagctgtt ttgaaatttc agtaagataa
    15901 actgccaatt ccgttaaagc tgcttcatca cctagctcac ttacttcttc tgttttttct
    15961 tcaagaatcg tttcgttgac tacctcgatc tcagctgttg gttcttcttc caaaatttct
    16021 tctacagtct gttctactgt ttcagaaact ttttcgctga tacttggctg gatcgaaagc
    16081 cgtgcattct ttcgacccat tcctaggaaa cctttttttc cttcttccag tatttcgatt
    16141 tcgacttggt ctttggccaa acccaaatca gccagaccta gttggacagc ttcgtctatt
    16201 gttgcagctt cataaatcgg cattagcatt acctccccta ttatttccgt tttttcttag
    16261 gattcattgc tttcttaaga gcacgttctc tttcgcggat tctccgtgct tcttcttcac
    16321 gctcttttcg aattttgaaa gggttgttga tcagcagcgt ttgtacaact tggaaggcat
    16381 tggaaactac ccagtataat gaaagaccgc tggctaaatt gacacccatc actaaaatca
    16441 ttacaggcat cacgaaattc atgatcttca ttgatgcatt cgattcaatt tgactcatgc
    16501 tagataaata cgtacttgca aaagtaaaga ttgctgctaa aatcggcaaa ataaatgttg
    16561 gatccgcgtt ccctaagttc aaccataaga aatgcccttg tttcaactct ggtacacgtg
    16621 aaatagattg ccataacgcc atcaagattg gcatttgtac taataaagga agacaaccag
    16681 catatggatt gacgccattt tctgcatata aacgctgctg ctcttctctt aacttacttt
    16741 gtgtttccgg atctcttgaa ctatattgtt gctgcagtgc ttttaatttc ggttgtaatt
    16801 cttgcgtttt acgcatgctc tttgtttgga aatgcatcaa cggcaatagg atcacgcgga
    16861 tgatcagtgt aaataaaatg atccctatcc ctgcattacc aaatgacaac gccttgatcg
    16921 cctcagcaaa gtaataaaca atataacgat cccaaattcc tgtactctgt gcgctgactt
    16981 ccccagttcc gcaaccggat aagacgatga ccaaactaac gataccggcc atcaaaagta
    17041 aacgtttgta tttcttcact tactcttttc ctctctgtcg attatttttg caagttttaa
    17101 gacgtggact aaattggagc gaacctcatc ataagtcaac cccttcacac ttggtctagc
    17161 aatcacaata aaatccaact ccggatcaat ttcttctttt atagaataaa ctgctgctcg
    17221 gatttttcgt ttgacttcat tgcggtttac cgcgttgccg accttttttc ctacagataa
    17281 accaactcga aaatgaggct gtcccggttt ttccaaacgg taaacgacga atttgcggtt
    17341 agcaaaagaa tctcccgtat ggaacacggc ttgaaactct cgttcttttt ttactcgata
    17401 cgcttttctc attagttttc tttccttttc tatctctcac ttacgctcat aatcatacca
    17461 taattacgat tgtcctttca tcatttccct aaaaaagagt ataaaaaaaa ccactgaaac
    17521 cccagtggcc taagctgaaa gaacttttct tcctttgcga cgacggctag ctaacacacg
    17581 acgtccattt ttagtactca tacgtttacg gaatccgtga actttttgac gtttacgttt
    17641 atttggttgg tatgttcttt tcatatattc cacctccaaa atgttcttat ctattttata
    17701 aacacagaca tactttgata gtataacgac aaagtagtga ttttgtcaat ataaaataga
    17761 agatcgattg ttaaaactta tccacaagat tatttttttc tatgggaatc ctatgtggaa
    17821 aaagttatgc acagggatta tccacagata ataaaaggct ggttttacac agaaaacaca
    17881 cgtgtaaaaa aagtaatcca caatcaggcg atttcctgtg tattccgtct cctaactatt
    17941 ggtatatctg tattttttat ccacagacgc aagaggaaat ctattggttt ttacgagttt
    18001 tccacctgtg cattagtggt gtggaaaact ttttttgaca actgtgtttt tctctttttt
    18061 ctttttgtcg attgtgtgga taattgtaat taaaaaattt ttttcttcgg tttctgtgga
    18121 aaactattga aaaaaatgct aaattagtac tagttattca ctttttaatg aaggaggagg
    18181 ccttatatgg tatccctcga tgctttatgg aatgaattaa aagcaacata ccaaaaggat
    18241 ttatcgcctg ctagctataa tacatggatc gaaacagcga accctcgaac ccttgatcaa
    18301 aaccagctcg ttgttgaggt tcctagcaag attcataaag aatattggga aaaaaacctg
    18361 gcgacaaaaa tcgttgaagt cggttatatg ttatcaggaa atgagattat ccctcgcttt
    18421 attactggcg aagaagcaga gcaagaagaa gttatagaag aaaaaaatcc aaaagttgtg
    18481 gcacctagtc cgctaaaaaa agccatgctg aaccctaaat atacctttga tacgtttgtc
    18541 attggtaaag gaaaccagat ggctcatgca gcagcgctcg ttgtagcaga agatccaggt
    18601 tctatttata acccgctctt cttttatgga ggcgtgggac ttggtaagac tcacttgatg
    18661 catgcaattg gtcatcaaat gctgcaaagt cagcctaatg ccaaagtaaa atatgtaagc
    18721 agtgaaactt ttgctaatga tttcatcaat tcgatccaaa acaaaacagc agaagagttc
    18781 cgtcaagaat atcgaaatgt tgatttactg ctagtggatg acattcaatt ttttgcagaa
    18841 aaagaagcta cgcaagaaga gtttttccat actttcaatg ccttgtacaa tgaagggaaa
    18901 caaatcgtac taaccagtga tcggttgcca aacgagattc cgaaactgca agaacgcctt
    18961 gtttctcgct ttgcatgggg gctgtctgtt gatattacac cgccggatct tgaaacaaga
    19021 acagcgattt tacgtaaaaa agcggcggct gaacgtctag agatcccaga tgacacacta
    19081 agctacatcg caggacaaat tgattctaac attcgtgaat tagaaggtgc gcttgttcga
    19141 gtgcaagctt ttgctgcgat gaatagtgaa gatatttcga ctagtctagc tgcagacgca
    19201 ctgaaaacgt taaaatcagg aaaaggacat cctcaattgt caatcctgca gatccaagaa
    19261 gaagtggcaa agtattatca catccaattg aaagatctca aaggaaagaa acgcgtcaaa
    19321 tctattgttg ttcctagaca aatcgctatg tatttggcac gtgagctcac agataattct
    19381 ttaccgaaaa tcggagctga attcggtggc aaggatcata caacggttat ccatgctcat
    19441 gaaaaaatcc agcagctgat ggattctagt gtcagtatgc aaaatgaagt atcagaaatc
    19501 aaaaactttt tactaaatta atttgtcgat tctatcaact gaaaatagca tgtggataaa
    19561 agaaaaaaac gttcaaaagt tatacacact ttttccacag ctgttttttc gtttctctct
    19621 tacgtttttt gagttttcca cagaatcaac atgccctatt acttttatta ttctttttaa
    19681 ctaataataa ataaacaaac attcaaagga gatacatcat gaaagttact ttaaaccgag
    19741 ctagctttat gcaggaattg caaactgttc aacgagctat ttcaagcaaa accacgatcc
    19801 ctattttgac aggtgtaaaa atcacactga cacaagaagg tttgactttg acggggagca
    19861 acgctgatat atcaattgaa acttttttgt ctgttgaaaa cgaaaaagca aatatgcaaa
    19921 tcgaatctac tggttccatt gttttacaag cacgtttctt tagcgaaatc attcggagac
    19981 ttcctgaaga aacatttact ttagaagttt tagaaaataa acaagtagcg atcacttctg
    20041 gaaaagcgaa ttttatcgta aatggattag atgcagataa ctatcctcat cttcctgttg
    20101 tcgaaagcca taaccagatg aaattacctg tacacgtatt gactaaacta atcaacgaaa
    20161 cagtttttgc tgtctcacaa catgagagtc gtccaatctt gacaggtgtg catttcattt
    20221 tatctgataa ttctttatta gctgtagcta ctgattctca ccgtctaagt caacgcgtga
    20281 ttccagtaga acaagcggct gatcattttg atattgttat tcctggaaaa agtttgatcg
    20341 aattatctcg ctcattaaca aatgaagaag aaatcgtcga aatcagcatt atggaaaacc
    20401 aagtgttgtt caaaacagaa acgatgtatt tctattcccg tttgctagaa ggaaactatc
    20461 cagataccaa tcgtttgatt ccttcaagtt ttaatacaga ggttgaattt tctgttccta
    20521 gctttttagc ggcgatcgaa cgggcttctt tactttctca tgaaggccgt aacaacatcg
    20581 ttcgtttatc tattcgtcca gatgcggttg ttttatacgg aaattcgcca gaaatcggga
    20641 aagtagaaga aagcttgagt tatacagcaa gcagtggcga tccattagat atttctttca
    20701 atccggatta tatgaaagca gcgttgcgtg catttggaga tatgagcatc aaagtgaaat
    20761 ttatctctgc tattcgtcca tttacattgg aaccaacgga agatggcgtt caatttattc
    20821 agctgattac tcctgtacgt acaaattaac aatgattttc aatgttcttg aaaatttaat
    20881 tgaaaagaaa tagaaaaaat aaaattttaa acagaaggcc ctaaaatctc ttttaaggct
    20941 ttctgtttat ttttgtttaa atcacctttt
//
LOCUS       DAGCYL010000029        20826 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.030, whole genome shotgun sequence.
ACCESSION   DAGCYL010000029 DAGCYL010000000
VERSION     DAGCYL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.030"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(17..1027)
                     /locus_tag="MB216_000849"
     CDS             complement(17..1027)
                     /locus_tag="MB216_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBT4525707.1"
                     /translation="MKKKIFVGALVALFLLPVNAFAYTINNEFNLGANEGSSQVSNNQ
                     YILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKETFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     TDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVNANGVSRPD
                     VNQSYSTYGNVGYHVSFNMRNFSNKKVYVMMRATNDPEGNTKDGAQDFHDKRWYLNIP
                     KR"
     gene            complement(1038..1235)
                     /locus_tag="MB216_000850"
     CDS             complement(1038..1235)
                     /locus_tag="MB216_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBT4525708.1"
                     /translation="MILPDKYYQIIKWAVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            complement(1249..1491)
                     /locus_tag="MB216_000851"
     CDS             complement(1249..1491)
                     /locus_tag="MB216_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBT4525709.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKNNNKWAWGYMIGLGITIIGYFLTKL"
     gene            complement(1526..1663)
                     /locus_tag="MB216_000852"
     CDS             complement(1526..1663)
                     /locus_tag="MB216_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBT4525710.1"
                     /translation="MFSFSDIKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            complement(1663..2070)
                     /locus_tag="MB216_000853"
     CDS             complement(1663..2070)
                     /locus_tag="MB216_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525711.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFGRRQQSQFFNFTENKWEEAVTQNYSKKLELLENLSIGLQVDNTALKKSNEELTEK
                     ADSMAQLNAKLMFNDLTINKKIEAIEKQIGGAS"
     gene            complement(2098..2622)
                     /locus_tag="MB216_000854"
     CDS             complement(2098..2622)
                     /locus_tag="MB216_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525712.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPYGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            complement(2622..2990)
                     /locus_tag="MB216_000855"
     CDS             complement(2622..2990)
                     /locus_tag="MB216_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525713.1"
                     /translation="MTDIVKVKQNDVQVYPQTHWDAVEGKPETIKGDKGDPGQAATIT
                     VGTVTSGTIASVTNAGTASAAKFNFVLPKGDKGDKGDPGANATTTAVATTTANGLMSK
                     EDKVKLDGLANITFEKVGTV"
     gene            complement(2995..3783)
                     /locus_tag="MB216_000856"
     CDS             complement(2995..3783)
                     /locus_tag="MB216_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525714.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITSEMYQKILNSGEGDYVLPVATI
                     DRLGGIKIGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGKI
                     NAEVTGFYVLPTASEFVKGGIRVGEGLTMTDDVLSADKQFNYTAGANISISNTGVISA
                     TGGGEGGGVSQEYVDQKMSEAYQNAQAYTDSKIPNVSFEKVGEV"
     gene            complement(3783..4691)
                     /locus_tag="MB216_000857"
     CDS             complement(3783..4691)
                     /locus_tag="MB216_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBT4525715.1"
                     /translation="MAKWNVVLSTTEPYNYVGMIQVRQGNKNSEVMEATITENGMPYD
                     LTGCKVYFESVVGDKYPVQLGTKVIDAKKGKIQYTFDQYSMQCLHRQTADFIIYRDDE
                     LIATTQDFSYFVIKAVSKTEGEMGSYWQTVEDLIADMTAFINENKGDFTDWMNARKKE
                     FEQWRQDQQNTFEAWREGQETDYLKWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(4657..5175)
                     /locus_tag="MB216_000858"
     CDS             complement(4657..5175)
                     /locus_tag="MB216_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBT4525716.1"
                     /translation="MIEEKGLNHLKSLLNQPIGNHQCYALSAEYAGVMIGPDMGAGTQ
                     YEIKVRHGNVFSAAEIGRAYPWSLYLWTVIAHPEYDQLVVGSIINWERNAKISDTFES
                     HEYYGHTGVIKGLENGRIQTYEQNAESGGIVAEYDREFFGSGQIASICIPPDFEKGVV
                     INGKVERSTQHN"
     gene            complement(5186..8008)
                     /locus_tag="MB216_000859"
     CDS             complement(5186..8008)
                     /locus_tag="MB216_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4525717.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNIKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLIGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGEMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFDIAMTSKGEIVANFI
                     KVGIINAEVLESSINDLGDVLKIVKGTLQIWNDNKKIMELTKKGMEFWNTKESIGTIG
                     TTDSAGNPFPGASTPTPLEDNSLVIRTNGDGKYILISPKEGKGWVILGNGTSILFGSL
                     NLQEKLNAFGDAEFMKNVNIRGKLTINGQEVFPGQGGSGNNDGGSWNGMYPPEVTSQA
                     DKFAWELWVMLLSRGYSKASIAGILGNVQGEAGAAMNPDIAQVGGPAYGIVQWDGSAY
                     PLVPPATWDGRTYVQNLMRAAGITEDYRTMSAQGKLLDWTMYNGQWLGIVQPASVSGF
                     KAMTDPAAAAYTFERNYERPATTHPERQGWAVNWYNKFKDLQVPSAGSILSTAKSLMG
                     YFHYSQPLRWNFGSVENPDRNGYADCSSFVWLALTKAGYKTATRGTLWYTGSMAADAR
                     GARQYLTEISPNEAKAGDIIIVNLGAGAGNDGHTAILAEDWKGYSTSIVEMGGMNSNG
                     VGIGRVDWSFGYLLNGGDVCLARAKK"
     gene            complement(8005..8709)
                     /locus_tag="MB216_000860"
     CDS             complement(8005..8709)
                     /locus_tag="MB216_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4525718.1"
                     /translation="MNPDEPNFIWKNRNAVIDMDCIIEVELPDIMPNKRYETYTVQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLDAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAIGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(8721..11030)
                     /locus_tag="MB216_000861"
     CDS             complement(8721..11030)
                     /locus_tag="MB216_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBT4525719.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAASLAAFGEVDEALDTIITKTGATGKVADSLGESFKKVGANTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAVMARQAIEAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFNEGVTLMGQFEQAGVDSGAALSSL
                     SKATVAYAKDGKTLSQGMGELQDKIKNASSETDAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKIADESQGSVGETFEATLDPIDKSNQAMNNAKLALADVGESVQISLLPFFE
                     MAIDALKSFKGWWDSLDQGTKNWIITLAGIAAVIGPALVVIGTLMSSVTKITAGVKDL
                     ATVWSGLGKLFGLSGGWFAVAVIAIAALVAGLVWAYNNVEWFRNGVNAFFQGVSDIAV
                     EVFNFMGGYISNIFGGIIQNFQNFFDAGKRIFTGFIDFITGIFTGDWERAWNGVVNIF
                     GGIFDGIAAMAKAPFNGMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANQQAIYDLGGVPI"
     gene            complement(11225..11689)
                     /locus_tag="MB216_000862"
     CDS             complement(11225..11689)
                     /locus_tag="MB216_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525720.1"
                     /translation="MARLADYGIHVDDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKTQAKRKVNKKNRKR"
     gene            complement(11689..12315)
                     /locus_tag="MB216_000863"
     CDS             complement(11689..12315)
                     /locus_tag="MB216_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4525721.1"
                     /translation="MGKQDTLYFEGLDDILIAMMATPDTVGTAPTYSEVVRLPIGTKI
                     GVKGNGTALEKWASSKMFRRVSRETKHEIALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANFVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWETLAKVGGGK"
     gene            complement(12322..12687)
                     /locus_tag="MB216_000864"
     CDS             complement(12322..12687)
                     /locus_tag="MB216_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBT4525722.1"
                     /translation="MPSNKNGFSEISDYLGNLSRVDPKKLSLESLEEAAKFYLEQLLP
                     NIPKSLLKKKHMSEQIKVVVDDDRVKVQFEETAFYWRFVENGATNQRAQHFASGTYEQ
                     NKEKIEEIMTKKILDLWEG"
     gene            complement(12677..13015)
                     /locus_tag="MB216_000865"
     CDS             complement(12677..13015)
                     /locus_tag="MB216_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525723.1"
                     /translation="MSISFDELKTALKSTKLPVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQISFFTLGTEQDLAVIEEALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCMENAK"
     gene            complement(13005..13331)
                     /locus_tag="MB216_000866"
     CDS             complement(13005..13331)
                     /locus_tag="MB216_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBT4525724.1"
                     /translation="MPKRQTNNLRWKAELLEIKTGTDENDRPTTVYESKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDTREMEL
                     SLAYVD"
     gene            complement(13309..13590)
                     /locus_tag="MB216_000867"
     CDS             complement(13309..13590)
                     /locus_tag="MB216_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBT4525725.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKALSAITPFIVQGVIQNAETTDQ"
     gene            complement(13592..14956)
                     /locus_tag="MB216_000868"
     CDS             complement(13592..14956)
                     /locus_tag="MB216_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBT4525726.1"
                     /translation="MNKKLLKKLQARHEQRLTELRGRIESGEVREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTNKQKDQQLRKAFANFVVGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLDPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFEKPLDLTAAGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGKPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(14968..15540)
                     /locus_tag="MB216_000869"
     CDS             complement(14968..15540)
                     /locus_tag="MB216_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBT4525727.1"
                     /translation="MKKKEIRTFDITNLSTRDVTENNSRIVTGYAAVFNSRTLLWEGL
                     EEVISPGAFSKALSNSDVRCLFDHDWGKVLGRTKSGTLRLEEDERGLKFEVELPNTTD
                     ANNLIESMSRGDIDQCSFGFIPTEETWDYNTDPVLRTLNEVELFEVSIVSLPAYQDTE
                     AALARSKQEVQQDIATRKKMIQKINGALNA"
     gene            complement(15512..16699)
                     /locus_tag="MB216_000870"
     CDS             complement(15512..16699)
                     /locus_tag="MB216_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBT4525728.1"
                     /translation="MEKRGYIEDMFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALSSPVVIGPDGNEVKDHHLLKILKNPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNDSDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQNVKGGDGNEKEGN
                     SDI"
     gene            complement(16763..18490)
                     /locus_tag="MB216_000871"
     CDS             complement(16763..18490)
                     /locus_tag="MB216_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBT4525729.1"
                     /translation="MIEPGVNYADLFAKEVRKKPGKYPKTVRLAVDRWYRWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFILEPWEKFIYSWMYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAATILN
                     NPILLEGTKIFKGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGNLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MDLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(18487..18939)
                     /locus_tag="MB216_000872"
     CDS             complement(18487..18939)
                     /locus_tag="MB216_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBT4525730.1"
                     /translation="MTTKAQRKAIVDEKVSAEKARILKIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRQITGGSTARKDEEITRPEEKPVDELAAHRNKWRKKAGTEK"
     gene            complement(19051..19431)
                     /locus_tag="MB216_000873"
     CDS             complement(19051..19431)
                     /locus_tag="MB216_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBT4525731.1"
                     /translation="MKYCQFDGCTNKIAKGIYCAEHKRSSKSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(19428..19814)
                     /locus_tag="MB216_000874"
     CDS             complement(19428..19814)
                     /locus_tag="MB216_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525732.1"
                     /translation="MAMIKIRTTITGTEYWDSEKKKTVVVPKGQEPDFEATEEKGILS
                     DGKTFVAVNGELITNSSEILDSDGNTAADFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAVRAKGDIINLIREAE"
     gene            complement(19795..20100)
                     /locus_tag="MB216_000875"
     CDS             complement(19795..20100)
                     /locus_tag="MB216_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525733.1"
                     /translation="MNDFHEAMLMANVPTNMGDVYKKAIEAENSPTGLRNNWNGTYAN
                     VQVDKDNRGIAMDVLVISLLSHTLPNLQETVEWYERMGAKVISTNYKGENQNGNDQN"
BASE COUNT         5685 a         4654 c         3398 g         7089 t
ORIGIN      
        1 atcgaggggc tattttttat cgtttcggaa tattcaaata ccaccgttta tcgtgaaaat
       61 cttgcgctcc atctttagtg tttccttctg gatcattagt tgctcgcatc atgacgtata
      121 ctttcttatt agagaagtta cgcatgttaa aagatacatg ataaccaacg tttccataag
      181 tactataaga ttggtttaca tctggacgtg aaacgccatt agcatttact cgtgctaact
      241 cttttccagt attatagtcc atgataaaaa tgtactcgta tttatagtta gcaatgtgcc
      301 agccagccac atgcaagttt gcgttttcga tttctccaaa ctgatcaatg tgggcgtaat
      361 ttgttccatc agtgagtgta ggatttgctg cacctgctcg tgttgggtca atgacaggct
      421 tgttctcaga agttgttgga ttttcatctg taaatccatg ggctaaatcg taagccagtt
      481 tttccttact cacacccatt tctgacaagt atccataagg atcagtgtga tcgccccaaa
      541 tgttttgagt tacccataaa tgtgatttga ttcctggttg gttataaggc gtgtccaatg
      601 ttaatggaat accatatttc attgctgaat ctcttgccaa ttcaacatat gctttatagt
      661 tcttttcaaa agtttcttta tcatgtgtgt gttgtaactc gatttgcaca ggactgttag
      721 cattagcata cgaaccagca ccgtactgta cataaccagg ttgtccaact tggtaaacaa
      781 ttccaccgtc tcccacaata taagcagtat aagcactagt ccatgaacgt tgcatatatt
      841 gcgcttcatt gcgtcctgtc gcagtttcgt ttgctgtttc atgcaataga atatattggt
      901 tatttgatac ttgagaacta ccttcatttg cacctaaatt aaattcattg ttgatcgtat
      961 aggcaaatgc gtttaccggc aacaaaaaaa gagctacgag agctcctacg aagattttct
     1021 ttttcatttg tttcctccta ttttttcaaa ttataagccg acacaccagt gataacgcct
     1081 aaaaatgttg ctgctgcatt gatagtcaga actgtcatat ctgttccatt ccatccatat
     1141 gcttttccta acgtggctac taaaacagat gcagctggta atactgttaa aactgcccat
     1201 ttaatgattt gataatactt atctggtaaa atcatttctt cgcttccttt atagtttagt
     1261 taggaaatag ccaatgattg taatgcctaa gccaatcata tagccccaag cccatttatt
     1321 attgttcttc atttccttga tgtcttctgc attgtttagt gctactgaat aggctttatc
     1381 tgccaaatct tttgcactat cagccttttc tctaagtgat tcgtagttgt ctaattttgt
     1441 ttcaattctt actaatctct ccacaacgtc ttggagcgct tcttctttca tgttccacca
     1501 actttccaac aaaaaaacgc atcacttaag atgcgctctc ttctttgcta ataattttat
     1561 ctgcttcttc gtctgtaatg catagtggaa cgaataatcg aacttgatcg tcagtaaaac
     1621 agccccaatc atacatcatt tttatatcgc taaaactaaa cattatgaag cacctccaat
     1681 ttgtttttct attgcttcaa tttttttatt aattgttaaa tcattaaaca tcagtttggc
     1741 gttcaactgc gccatcgaat ctgctttttc cgttaactct tcatttgatt ttttcaatgc
     1801 agtattatct acctgtaaac ctatagataa attttctagt agttctaatt ttttagaata
     1861 attttgtgta actgcttctt cccacttatt ttcagtgaag ttaaaaaatt gcgactgttg
     1921 tcttcttccg aaaccttcat ccgtttcatc ttccttcttt tcaaacaaaa taggtggaac
     1981 ttcgacaaat ggtaaagatg ttggaaagtt atcttctact tgatgttctt catatcccat
     2041 tggatacaat actttataaa ttgttttcat tttactcttc ctccaattag taatctgtta
     2101 agccggccaa ggatcttgag tgatccacat acctgaaata tatgaactac ctgaacctga
     2161 tttggcttgt atgatgctac tttgatcaat gaatgcacgt gcatccgctg gctgacttgc
     2221 atttctaact agtgaaagcg cggtttgcgc tggatagcct tgatcacgtt tgaaaccgta
     2281 cgggattttt aaaatccaac gagtttctga tccttcgggc catgtgccac atttaaaatt
     2341 aaaagtaaga aatacgatgt ttccgattct gattattttt ccattcacat cagttacgtt
     2401 tgttgtatca cttcgatcag ctagtgtgat agctcgctcg atcatacctg cttgcacagg
     2461 caatccattg aattgcaatc cgtctttaaa atttttcgtt cctaagaccg tttcattacc
     2521 tgtggctttt actaaaacac cctccacacc atcaattcct ttggcatgag ttttcaaata
     2581 cttagcaact ccgtcttctt ttaattgcac aatatcagcc attaaaccgt ccccactttc
     2641 tcaaatgtaa tattggctaa tccatcgagt ttcactttat cttctttgga catcaaacca
     2701 tttgctgtcg tcgtagccac agctgtcgtt gttgcattgg ctccaggatc gcctttgtcc
     2761 cctttatcac cttttggcaa tacaaaatta aactttgctg cacttgcagt accagcattt
     2821 gtgacactag ctattgtacc actcgttact gtccctacag taattgtggc agcctgtcca
     2881 gggtctcctt tatctccctt gatagtttcg ggtttaccct caacagcatc ccaatgggtt
     2941 tgagggtaaa cctgaacatc attctgtttt acttttacaa tatctgtcat ttgtctatac
     3001 ctcccctact ttttcaaacg atacatttgg tattttggaa tctgtataag cttgtgcatt
     3061 ttgataagct tcactcattt tttgatcaac atattcttga gagacgccgc caccttctcc
     3121 accacctgtt gcagagatta ctcctgtgtt tgaaatactg atattagctc cggcagtata
     3181 gttgaattgc ttatcggccg ataaaacatc atcagtcatc gttaaacctt caccgacacg
     3241 aataccacct tttacaaatt cggaggcagt gggtaaaacg taaaaaccag tcacttctgc
     3301 atttattttt ccatcttcat caatagtgat gttttctcct gcagtatagt ttttcagtga
     3361 atcgagtttt tcttttaact ccagcgaaaa attgacatca gattgtctga ctgcagagac
     3421 ttttcctgtc tcatcaatcg tcaataattc accaattttt atgccaccca aacggtcgat
     3481 agtggcaact ggcaacacgt agtcaccttc tccactgttt aaaatttttt gatacatttc
     3541 tgacgtgata atgccgtcat cttcttcact tgcatagggt aattctgtga gaacattctc
     3601 taatcccaaa tcttctttag taatgacgac ggctccaatt ttgccattaa cagataaaac
     3661 tgttgcttct cctgctatta tttcttctaa cccaagaact gcggaagcat gtgtgattgg
     3721 aaagaattgg cgttgtacac ctgttttttc atccgtttcc atcattcgtt tagttttagc
     3781 cattagatca ccccaacttt ctcaagagtg aacacatttt gctttggatc atcaacggtt
     3841 gcgatgatca aggctccttc ttcaatcggg aattctacag ttccaacttt ttcgacttcg
     3901 tgattatctg aaaagagatc atcctgcaaa atatcagtca cttcaatctc accgtattca
     3961 atcgtgaaaa gtgttgctcg taatttctga tatagataat ccatatctgc cagcaagcgc
     4021 tctgaaatag acgcatgacg aactccttga atatcaacac gtgcatccat taattcggct
     4081 aacattactc cacctggatc gatagacttc aaaatatctt tgattgattc aaaccatttt
     4141 aaataatcgg tttcttgtcc ttctctccat gcttcaaatg tattttgttg atcttgtcgc
     4201 cattgttcaa attctttttt gcgtgcattc atccaatctg tgaagtcgcc tttgttttca
     4261 ttgataaaag ctgtcatatc cgcaattaaa tcttcgactg tctgccaata cgatcccatt
     4321 tcaccctctg tttttgagac ggctttaatc acaaaatagg agaagtcttg cgtcgtagca
     4381 attaattcat catctctata aatgataaaa tctgctgttt ggcgatgtag gcattgcatc
     4441 gaatattgat caaatgtgta ctgaattttt cctttttttg catcaatgac tttagtacct
     4501 aattgaactg ggtatttgtc tccgacaact gattcaaaat agactttaca accagttaga
     4561 tcatagggca ttccattttc agtgatggtt gcttccataa cttcggaatt cttattgcct
     4621 tgccgtacct gaatcattcc gacatagtta taaggctcag ttgtgctgag tactacgttc
     4681 cactttgcca ttaattacca ctcctttctc aaaatcaggc gggatacaaa tagaggctat
     4741 ctgaccagat ccgaagaatt cacggtcata ttctgccaca attccacctg attctgcatt
     4801 ttgttcatag gtttgaatac gcccattttc cagacctttg attacacctg tgtggccgta
     4861 atattcatgg ctttcaaatg tatcactgat ttttgcgttt ctttcccaat tgataattga
     4921 gccaacaact agctggtcat actcaggatg agcgatgacc gtccataaat ataatgacca
     4981 tggataggct cgtccaattt cagcagcgga aaatacattg ccatgacgta ctttaatctc
     5041 gtattgtgta cctgccccca tgtcaggacc aatcatcaca ccggcatatt ccgcagataa
     5101 tgcataacat tgatggtttc cgataggttg attcaacaaa cttttcaaat gatttaatcc
     5161 tttttcttcg atcacaaaat cacctctatt tcttcgctct ggcgagacaa acatcgccgc
     5221 catttaataa atacccgaag gaccaatcga cgcgaccgat accaacacca ttggaattca
     5281 tgccacccat ttcaacaata gaagtgctat atcccttcca atcctctgct aaaatagcgg
     5341 tatgtccatc attaccagca ccagcaccta aattaacaat gatgatatct ccggctttcg
     5401 cttcatttgg agatatttca gtcagatact gacgtgcccc tcttgcatct gcggccattg
     5461 aacctgtgta ccacagagtc ccacgtgttg cggttttata tcctgctttt gtcaaagcca
     5521 accacacaaa agaagagcag tcagcatacc cattacgatc aggattctcg acgctaccaa
     5581 aattccaacg taacggttgc gagtaatgga aataacccat caagcttttg gctgtactga
     5641 gaatactgcc agcagatggg acttgaagat ctttgaattt gttataccaa ttaactgccc
     5701 atccttgtct ttctggatga gttgtggctg gtctttcata gtttcgttca aatgtataag
     5761 cagcagctgc tggatcagtc attgctttaa atccagaaac acttgccggt tgaacaattc
     5821 ctaaccattg accattatac attgtccaat ctaataactt tccttgagct gacattgttc
     5881 gataatcttc tgtaattcct gcagctctca tcaggttttg aacataagtc ctgccatccc
     5941 atgttgctgg aggaaccaaa ggatatgctg aaccatccca ttgaacgatt ccataggctg
     6001 gaccgccaac ttgtgcaata tctggattca ttgcagcacc ggcttctcct tgaacatttc
     6061 caaggattcc agcgatggat gctttggaat accctctaga aagaagcatg acccataatt
     6121 cccatgcaaa tttgtctgct tgactggtaa cctctggtgg gtacatgcca ttccaactac
     6181 cgccatcatt atttccactt ccgccttgtc cagggaatac ttcttgtccg ttaattgtga
     6241 gttttccgcg aatattgacg tttttcataa attccgcatc accaaaggca tttagctttt
     6301 cctgtaggtt taaacttcca aataatatag aagttccatt cccaagtatt acccaacctt
     6361 ttccttcttt tggtgaaatg agaatatatt ttccatcgcc atttgttcga atgactaatg
     6421 aattatcttc taacggtgta ggagtggatg cgccaggaaa aggattgcct gcagaatcag
     6481 tagtaccaat tgtgccgata gattccttgg tgttccagaa ctccattcct ttcttggtta
     6541 gttccatgat ttttttatta tcattccaaa tttgtaacgt acctttgaca atttttagca
     6601 catcgcccag atcattaatt gagctttcaa gaacttcggc attaataatc ccaactttaa
     6661 tgaagttagc tacgatttca cccttcgacg tcattgcgat gtcaaacggg ccatttactc
     6721 cgttgtctga ataacctaag ccatttaaat tccaacgcca cacacgcttc gcattcgcta
     6781 cattgggagt atccataata agaatctctg atggagcttt ttctggacga aaaacgacat
     6841 gcccaccaga attaccagtg atccatgccg tcgcattcaa cacattttga actaacgttt
     6901 ctgttcgatt gtcgattttc tttttcaatt cttgtgcttg agtatttact gccgaagtgt
     6961 acagtgataa atcattacct aaaacaatat ccttatattt cccaagcgtt gggaaataag
     7021 tgtattcgac catacgctct tttatctcga tgtctagctc tttcgcccta acatgagcga
     7081 catcaccaaa gtgtaaagac gctagctctt gatacatctc accgtattcg agtgtgtgtt
     7141 ccaacgccac catgctgact gtatgagtag cttttggctc gtgaatacgg tcattgtcaa
     7201 ataaggtttt gccccatttg atcaattcat caactgtttt acaatcacca ttttcacgtt
     7261 tggcaatacg tcgattctca ttggtcactc catcaatttc taaataacca tattcgatag
     7321 gttctttgtc ttcatcataa tcgttatcag gaaccccgcc aatcaaatac aggctattta
     7381 cgatggattc ttcatctatt tcttcttcga ttgcttctaa attgatacca aaatcgattc
     7441 ggaaaccatt atctgatcca atctgtttta ccagtttcaa atcatagtta tccatatcaa
     7501 gttccgcgct agtcacgcca gtcaaatttt ggttaccgtt gtttgaacca atgattgctt
     7561 caactggcgc gacttgtctt gctgtaaatt ggtgcgttgt ccctacattc gaaagatagt
     7621 taaatttttg atcaaatgcc aaactgtttt taagattggt catgatctga gaaccgttgc
     7681 cgttatccgt gaatgactta acgataaagt ttttattggc cataaatcca atatgacgag
     7741 cagttacaga aacagacgtc aaattctttt tgatattgta aatttcgaag tattgccatg
     7801 atccatctgg caccttcgct tttatgaaat ttcctttttt taagtaagac cgatactgtc
     7861 cgcttcttga atagttacca taaaaacgat attggccatt caacacacga ttgatttctg
     7921 gcaaatcttc ccagtcaact aacgacgctc cgttcacgct caaatcatct ggcatttttt
     7981 tataagcata aataaattct tgcgtcacag atacacactc ctattccaaa accgcacttc
     8041 tttaaatttt ccggatattt ttacctgatt ccattcaggc tgcaatactg gccaatcacc
     8101 acgcgtaaat aaattcaaac cttcttgtat tgcttttcca agctgagtat caaccacgat
     8161 tgttgcagct aacgtattaa gtattgttag gcttttatcg ccgatagcaa tcgtaatatc
     8221 tcctccgttc gattctattt ccagataggg atgtgcaatt tcgtcaccat gatcaaaaat
     8281 atccataaca ctagaatgaa aaattttagg cgcttcaccg atttttcttt tgagtggctg
     8341 acaacgaaaa gtaacatcga atgtataaaa gaagccccac tcgttttcga atgggacttc
     8401 tttatccatg ctgcagatag catctagata cttgtctgga tcattatgag tgattaattg
     8461 gcttttacca gtaagccatc ttttgacttc cctcagtttg gaatgtggga tagtgatccc
     8521 ttctatttct aaatcaaaag gttcataatc atcaaacgtc tccgtcaatt ctccgcttcg
     8581 gccctgaacc gtgtaagttt cgtatcgctt gttcggcata atatctggaa gctctacctc
     8641 aatgatgcaa tccatatcaa ttactgcatt ccgatttttc caaataaaat ttggttcatc
     8701 tggattcata aattgtcgac tcaaatagga acacccccca aatcataaat agcctgttgg
     8761 tttgctttag caaatttacg attcatacga tctaactcag atggattgtt tgcatcgact
     8821 ttaccaatgt gtatatgctg ttcaatgcta cctccggaaa ctcttccgct gattcctttg
     8881 cgtttttctt cgtcagataa tggggtaact gttgtttttc cattttttgc agtcagtaac
     8941 tctggaccag cttcaccaac gattgcttga ccattgatta gatgaccgcc ttgtgctaaa
     9001 taaggcagtt ttgaaataga aaatgatttt ccacctacac caggaaccca ttttggtatc
     9061 tttatattat tcaatccacc aataaatcca ttgatcaatc caatcattcc attgaatggt
     9121 gcttttgcca tggctgcaat tccatcaaag ataccaccga aaatgtttac aacgccgttc
     9181 caagctcgtt cccaatcacc tgtaaatatg ccagttataa aatctataaa tccggtgaaa
     9241 attctcttgc ctgcatcgaa aaagttttgg aaattttgta tgattcctcc aaaaatattg
     9301 ctaatatatc cgcccataaa gttaaacact tctaccgcta tatcagaaac gccttggaaa
     9361 aaggcattca caccgttcct aaaccattca acattgttat aagcccaaac tagcccagcg
     9421 actaatgcgg ctattgctat tacagctacg gcaaaccatc cgccagataa tccgaataat
     9481 ttgcctagcc cactccacac agtggccaga tctttaaccc cagcggtgat cttagtaacg
     9541 gaactcataa gcgttcctat gacaaccaaa gctggtccga ttactgcagc tataccagcg
     9601 agtgtaataa tccagttctt agtaccttga tccagagaat cccaccaacc tttaaaactt
     9661 ttcaaagcat cgatcgccat ttcaaagaaa ggcaataagc ttatttggac ggattcgcct
     9721 acatcagcta aagctagttt cgcattgttc attgcttggt tagacttatc aattggatct
     9781 agcgtggctt caaatgtttc tccaaccgaa ccttgacttt catcggcgat ttttgccaag
     9841 tcttctaggt ttaacgttcc tctacgaatt gcatctgcca ttcttggacc acctttggta
     9901 ccaaaaactt ctgcagcagc gttgattgca tctgtttcag agcttgcatt tttgattttg
     9961 tcttgaagct cacccattcc ttgagaaaga gtcttcccat cttttgcgta agcaacagta
    10021 gctttcgata gactacttaa tgctgcacca gaatcaacac cagcttgttc gaattgcccc
    10081 attaaagtga caccttcgtt gaaacttagt cctaattgct tgatctgtgg cgcaccgtca
    10141 attgctttct gcattaaatc atcaactgat tgaccagtat tttgagaggt tttagttgta
    10201 acatccagta cagaatttaa atcgtcatat tccagaccgt atgcttcgat tgcttgtcga
    10261 gccattacgg ctgattgaga gacatcagtg tcgttaatct cagcatattg aaggagataa
    10321 tttgttgagt cttccaactt tttatccata aatccaaact gagtattcac ctcaccgatc
    10381 gcttcaccga ccgtttgtaa tggtaaatga gtgtttgctc ctactttttt gaacgactcc
    10441 cctaatgaat cagctacttt gccggtagcg ccagttttcg taataatcgt atctagggct
    10501 tcgtcgactt cgccgaatgc agcaagcgac gcagctccag ccgctacaat aggcgcggtt
    10561 actccaacag acattttttc accaacgcct tttactttct cacctgtttc ttcgattttc
    10621 tggagttttt ttgctgtatc aacagaaacg tcaccttgtt ctttaagtgc atcgttcgtc
    10681 tgttctaatg ctgtacgaag tttattttct ccggtttcag aattgagtaa ttgtttataa
    10741 agtttttctg attcagcaga atattcaccg gtttctttga cagatttttc atattcctct
    10801 ctcagcattt gagatcgttg ttcagccaat cctaattgtt tttctaattt ttgtttagcc
    10861 gctcgcaatt tttctgtttg agttgcatcc ttgtccatcg cagacacttg gtttttgtac
    10921 tcggcagcgg ctaagttcat ttctttgttg atttctttga ttgttttcga ataactgact
    10981 tcgccattcg ttttaaaatt aagaacgaca tcagattctt taccagccat ttatcttagc
    11041 gctcctttct accaccaagg cgatttatcc atagttacag attgaggtgg ttcaaaatcg
    11101 gtgttttgtt gtaaccactg taaataagat ttgagccaca agttaggtgt agacttcaaa
    11161 aagaaactct cactccaatt caatagagtg agagcaacgt aaatataaaa actccaagga
    11221 gttcctatct cttccgattc tttttgttta cctttctttt tgcttgcgtt ttttgaaagt
    11281 cttgtggctt cttggatttt tttaggtctt ccacctgaaa tgtctggcta gcaaaaattt
    11341 ccatacaggt actataagca gacaacacct ctccactcat tcccaaaaat ttgaaaatag
    11401 tttctggatc ttcctctaaa ccaccagtac gcagcatggc ataaattaag gcgcgcatga
    11461 tttttaggtc actaggcgat aagttagcag aagaaatttg tccttctttt ttagatagca
    11521 tggcattcat atcagattcg aattgcgaat aatctccacc atacacatct gctataaatt
    11581 ccatcgtttg catagtaaat gaaataggga attctgcgcc ttgaataatg acaacagcag
    11641 aattctttaa atcatcaaca tgaatcccat aatcggcaag tctggccatt atttgccacc
    11701 tccaacttta gccaatgttt cccactgttc ttcatcgtat acaggttgag caatgaattt
    11761 attaaataat tcgagagaag ctccgtctct attcgaatca aaacttgcat acatcacatt
    11821 attgtatttc agtccgatgg aaacaaaatt tgcagttaca tcgtcgattt ttgtctcttc
    11881 ttctgcagtg gcatactctt catcaattac attagatagt tgtgtgttag gataccatac
    11941 cgctttttta ccgccattct caatatttcc gatgaatcca aaggcaaaat acggaaattc
    12001 tcgtgcggta tttttgctaa acgttactcc acttttagca actaatcctt ttatttcatc
    12061 cattacttca atcggaatac ccacatgatc caacgcaatt tcatgttttg tttctcgcga
    12121 tacacgtcgg aacattttac ttgatgccca tttctctaac gctgtaccat tacctttgac
    12181 gccgattttt gtaccaatag gcaaacgaac aacttcacta taagttggcg cagtcccaac
    12241 ggtatcaggc gttgccatca tcgcaattaa aatgtcgtcg aggccttcaa aatataaagt
    12301 atcttgtttt cccaattaaa atcacccttc ccataaatca agtattttct ttgtcataat
    12361 ctcttctatt ttttctttgt tttgttcata tgtcccactt gcaaagtgtt gggctctttg
    12421 gttagttgct ccattttcaa caaatcgcca ataaaaagca gtttcttcaa actggacctt
    12481 cactcgatca tcatcgacca caacttttat ttgttcgctc atatgcttct ttttaagcag
    12541 ggacttagga atattaggaa gcaactgctc taggtaaaac tttgcagctt cttctaaaga
    12601 ttccaatgat aatttttttg gatcaactct agaaagattc cctaaataat ccgatatttc
    12661 agaaaaacca ttcttattac ttggcatttt ccatacacct cacatatgtg taatagttgg
    12721 ttacggtatc gtcgttctca tcaccttgta tacctgtaaa atctgaatat ggaataccag
    12781 cattttgtaa cgcttcttca ataacagcta aatcctgttc tgttccaagt gtgaaaaaag
    12841 aaatttgata atacggtaat tttttataga ctttaccgga tgccattttt ttactgttac
    12901 tcacatttga gtacacgatg tatggataca tcgtccctaa tctggcttta tctctgaaca
    12961 ctggcaactt tgttgatttc agcgctgttt tcaattcatc aaagctaatc gacataagct
    13021 aaactcaact ccatttctct tgtatcagga ttcgtataaa tgcgagtaat gttatacgtt
    13081 acagaatcaa ttttgagcgc acttaatttc tctgtgatgg atttatccca tctgacttta
    13141 attcgtctga caacgtctgt cttggcttgc tgtgataaat atttttcttg agaagtcaca
    13201 ccgagttctt catagaatat tagacgcttg gattcataaa ccgtagttgg acgatcgttc
    13261 tcatctgttc ctgttttgat ttctagcaat tcagctttcc atcgcagatt attggtctgt
    13321 cgtttcggca ttttgaatca ctccttgtac aataaatggc gtaatagcgc tcaaagcttt
    13381 gtctaactca tcttcagata ctcggtattc gtaggcaatg ccggcaacca tcaaaataag
    13441 atattcttgt tggccaccag ttgctgtttt gacataatct tttgccatat ttaaataaaa
    13501 agagagcaaa gaatcatcca tgccctcttc aaaatgaata tgtgatttga atttttcctc
    13561 taaagacaat tctttagttt gctcttccat cttaaccacc aactggtttt gtaatttcgt
    13621 agcgatatac tgccggttca aatggagaat aaaccaattg accatctagc aagttgtaaa
    13681 tttggaatcc aatttgattt ttaccagaga atttttcaac aagtttttga atttccaagg
    13741 caccaataac ttcttgaatt ttaaatgcag aaaaatcgcc aaaatataaa actggtgtgt
    13801 ctggtttacc ctttttatct gctgcatctg tccaatccac aggatagcca actaattggt
    13861 aacctattcc accttctgct tgtgtaaatg gacgcaacaa aggaaaacca tcatctgttt
    13921 tcattttttc aatagcagtc aaagcagctc gattaataat aaagcgtccc tttttcatca
    13981 cttctgtcac tggtgtattt ttaaattcga ttaatgcatc atataatttt tgcccagcac
    14041 ctgcagcagt tagatctaaa ggtttttcaa atgctacagc ctttttggct aatgcaccag
    14101 gattttcatt tccagcgtca tcaccattga acatataatt gatttcttta cgcacataag
    14161 cttttttcaa ttcttccaca acaatatctt caactggaac accagacatt tttagtaatt
    14221 ttttagttac tgttgccaaa gcatcgaatt cggcaggatc aagcaaaatt tcatcaaact
    14281 gaatagctgt ttcagcaata tcagttgaac gctctttctt gtttacattc gcatctgctt
    14341 tcttcacaag aattggatat ttgacatctc ctgatgttcg caccactgtt ccgtatttac
    14401 gaagtaaatt ttcttcttga gcataagtaa taacttcaga tgcaattact tctgggacag
    14461 taactgaacc gttgccagct tcaatcccta aagctcgagc ttctgcttca gaaatatttc
    14521 caactacaaa attagcaaat gcttttcgta gttgttgatc tttttgttta ttggtcattt
    14581 ttgcacgtgc ctccaatccg ttcttaattg atccaagtaa tccatctcgt tgctgttgag
    14641 taatcattcc agaacgattt tctgaactat cttcactgtc tgaatcttct tcttgatcgt
    14701 tgtcttcacc agaacggctt tcgtctgaat ccgtactatt cgattgatca tctgtattgt
    14761 cgtctgtttc atcagttcca gaatctgcgc ctaattcgtc tttaattccg ttcaattcat
    14821 caataacact gtcaatttct tctttcacgg cttctaaatc tgcttcacgt acttcaccag
    14881 attcgattcg tccgcgtaat tccgttaagc gttgctcatg acgagcttgt aattttttca
    14941 ataatttttt gttcatggtg tatcctccta tgcgttaagc gccccgttaa ttttttgaat
    15001 cattttttta cgtgttgcaa tgtcttgctg gacttcctgt ttgcttctag caagtgctgc
    15061 ttctgtatcc tgataggcag gaagagaaac gattgatact tcgaataatt ccacttcatt
    15121 caacgtacgc aaaacaggat cggtgttgta atcccacgtt tcttctgttg gaataaaacc
    15181 gaagctacat tgatcaatat ctccacgact catagattcg ataaggttat tagcatcggt
    15241 tgtgttgggt aactcaactt caaatttcaa cccacgttcg tcttcttcaa gccgaagtgt
    15301 tccacttttt gttcgtccta gaactttccc ccagtcatga tcaaatagac atcggacgtc
    15361 cgaatttgat aatgccttcg aaaatgcacc aggagaaatt acttcttcta agccttccca
    15421 aagtaacgtt cgactgttga acacagcagc atacccagtt acgattcgac tattattttc
    15481 tgtaacatct ctagtactca agttggtgat atcaaatgtc cgaatttcct tctttttcat
    15541 ttccatcacc ccctttcacg ttttgatcat tcgttggtaa ggaatcatct gttgcatttt
    15601 tctggccaat cctagacaag tcatttgaaa tatagatagc ttgtgtttct ggagtgttct
    15661 gtttaggaaa accaagcatt tctgccacat tatctggact tgtaatccca gtacgaacaa
    15721 tgttgtagcc aatatttgtt tttgttgagt aaggaacaaa atccaaaatg ttaattttcc
    15781 attccactcg atagccagaa ttaggcataa aaaaaagagc ggtgtaatgt tcgctcttgt
    15841 tcttcaatat tggtttgatt gctttgttgt gcagatacat catcgctttt tcgatgtctg
    15901 ttttcatcaa cgattgatac gtatcaacat tgattcctaa aaatttacct aagtcttttt
    15961 tataaacacc taaatagttc aaaatagccg cgtcatcaac aggacttttt aacgtctcga
    16021 tggaatatcc ttttcccaga ggaatcatct taacagaatg atcactgtca ttttgcgttc
    16081 cttccagttg atccaatata gctttgacaa ttttcgtttg ggcgctatta tttggattga
    16141 tgtgggcgtc cagtttaagc atgaacgcga gtaaaccacc tttagtatat ttgtccgtca
    16201 aaactttttc agcgctcaga acgccttcca gagtgtttct tgcaaggtca attattccag
    16261 caccttttaa tgaatcagtt ccgatgttct taatatgtcg aatcatttga ccaggtattt
    16321 tttgtccatt catttcaaat tcttcttgaa gtcgttcatt gattttagtg gttacaccgt
    16381 atgccaaatg aagctggtcc cgatctgtta atgggaatgt ctcaccattg atcagtaggg
    16441 tatttgtttc caatttggta aattcgaatc cggtcaaata atcattggga ttcttcaaaa
    16501 tttttagcaa gtggtggtcc ttcacttcat taccgtctgg acctatgaca acaggtgagg
    16561 acaacgctac ctggtttgag atatcctgga ccaattcata aacatcagaa gattccatga
    16621 tagaggaatc tgttacatat ctttgaccgt aacgcgtata gtggccaaac atatcctcga
    16681 tgtacccacg cttttccata aaggaataga ctgcattcga taaccgatca cgtaatttca
    16741 atatttctca ccgcctttct atttatctat agatggaact aaggtaatca tctaattcat
    16801 ccgaatcaat atctgtcatc tgattcatcg tttccttatg accacacaaa aacgccacga
    16861 aaccatcaat ctttttcttt gattgacgtt tacttggcgc tttttgtccg ttgatgttag
    16921 taattgctac aacattcaat gtgcaataaa ggaacaatgg attatcaaat tgaattcgtt
    16981 tctcataaaa caaccgttcg acatcatcaa aaggagcgtt caacacttta ggatattggg
    17041 caacctcaac gcattccaat cctaagttct ccaatttctc aacaagtttg tcgctcatcg
    17101 ctggatcata attcacttgt tgtatatcgt ataaatccat gcagtcttcg atgtactgca
    17161 agatttgatc ttgatcaatc attttaccat cgcaaaattc aacaaaacct tgttcagcta
    17221 aatcgctgta aggcacgtta tcttcttttt ctcgaaactc taaatcttca ttgggaataa
    17281 aataaagctg cttcacttta aggaccgctt ttccatcttc atcccacgtt gggaagttta
    17341 aagacacaca ggtcaaatct cgtgtgcgtg ataaatccaa accaatgtaa caaggttcac
    17401 cacttaaatt tccaagctct tgggtagtaa ccaaacacgg ctctacttga tcctgttcaa
    17461 agaaattatc cgcaccgttt acaaatacat ccaagtgctt cgttagaaac tcggcttttg
    17521 aatgtgctga acgttgtgcc gttttaaacg ctgactccaa ggcggaaaga tcaactgata
    17581 ttccccagtt aggattgcac atttcccaaa cttttctatc cgtccaatca tagtttttat
    17641 ttggttcata gattagaaca aagttcgaat cattatcatc acgtttcaac acttcttttg
    17701 cttcacgata tacgcgcatt ccaacagacg atgaaccttt cccagcagtt gaaatattaa
    17761 acatcaatgg ttgtggcaaa gagatttgtg cagacttaaa attgtcatac tgttccattt
    17821 tctcttgctt atgcaattca tcattcaata caaaatatgg attagaaccc tcaatgttat
    17881 cgatattctt cgtttgaaca ataaatttat tcgaataagc catatcttca tgaatataat
    17941 cataagtaat actagaaaca gttccctttg gtcctttgaa tattttagtt ccttcaagta
    18001 ggattggatt gtttaatatt gtagctgcaa aaggtttggc agcatattga gcttgagcaa
    18061 agtcggaagc gcacgcataa caatcgactg acaaagcacc ttctccatac attgcatagc
    18121 ctaatgaacc gacagcaatc aatgttttac cgttcttttt agggatttgg acatacgctt
    18181 cacgagtaac acggacaatt tgcccttttt cattttcttt aacccatcca tacatccacg
    18241 agtaaataaa tttttcccaa ggttctaaaa tgaatggctt tccaaccatc tcaccttttg
    18301 tgtgaacaat aaacgactca acccagtcca tcatttcatt cgcacgatct acatcaaacc
    18361 aaatatcttt acgttttttc caccgatacc aacgatccac tgccaaacga acagttttag
    18421 gatatttccc aggtttcttt cttacttctt ttgcaaataa atcggcataa tttacaccag
    18481 gttcgatcat ttttcagtac ctgccttctt acgccatttg tttctgtgcg ctgctagttc
    18541 atctactggc ttttcttctg gacgtgtaat ttcttcatct tttcttgcag tcgatccgcc
    18601 agttatttgt ctaccagttt tagccttatt cgttagcccc aacaaatcta gagctttcat
    18661 ttttttatct gcccaagttt ctacttgctg cgccaatgga tgcttcgatt gattagtggc
    18721 ccctgattta ttcgtgaatt tttgcgtctc cggaaagcct ttttccttcc acaaaagata
    18781 tttgtgttgg taaatttcaa aaatatccaa atatgattcg attaatggat caagagtgat
    18841 ggtgtacaaa tcagacaaat tcattatttt taaaatacga gctttttcag cacttacttt
    18901 ttcatcaaca atcgctttac gttgcgcttt agtcgtcata cttgtataca cccccctttg
    18961 ttttttgaaa aatttgacct aacgatgcgc gtgactcccc gctaccctat ctccccacgc
    19021 gaaaaaattt tgaaaatgga taggggggct tcaattgaaa tacgaaggga aaactttttt
    19081 gtcttccgtt tcattttcaa caattggatg acattttgaa cataaaagca tgagattgtt
    19141 tggatcaagc ttaagcagtt cattgtcttt gatgggtaca atgtgatgga catgtgccct
    19201 cttgccgaag ataaactgac cgcatcgctg acagtgacca ccttctcttt cataaataaa
    19261 ttgacgcata tccttccatg cctgtgttcg atagaatggt ttgttctcat gatgataaac
    19321 agactttgct tgttgcttct tcttacgtga tttgcttgat cgcttgtgtt cagcacaata
    19381 gatccccttt gctattttgt tcgtgcatcc gtcaaactga cagtacttca ttctgcttca
    19441 cgaataagat ttatgatatc gccttttgca cggacagcac caggaatatc aatgccatgt
    19501 ttcttagcat atgcacgtaa ttcttttgca gtcatattgt ccagttcatc cgtatcttca
    19561 gtggactgat cattagtagc ttcgtctcca tcaaaatcag cagcagtgtt tccatcgcta
    19621 tcaagaattt cactgctatt agtaataagt tcaccattaa cagccacaaa cgttttacca
    19681 tcacttaata ttcctttttc ttccgttgct tcaaaatcag gttcttgacc tttcggaact
    19741 acgacagtct ttttcttttc tgaatcccaa tactctgttc ctgtgatggt tgttctaatt
    19801 ttgatcattg ccattttgat tctctccttt gtaatttgtg ctaattactt ttgcacccat
    19861 tcgttcatac cattcaacag tttcctgcaa attgggtaaa gtgtgggata ataaagaaat
    19921 aaccaacaca tccattgcta tcccacgatt atctttatct acttgcacat ttgcataggt
    19981 gccgttccaa ttgtttcgta aaccagtagg actgttctct gcttctattg ctttcttata
    20041 gacatctccc atattcgttg ggacattcgc cataagcatt gcctcatgaa aatcattcat
    20101 agataaaact cctttcaaaa taaaaagacc actcaaagag tgatctaata tgtaatgcac
    20161 aggcagggac gtttccgatc ctgtgcttga gtcatttgac gattcatttg taccgaatcc
    20221 caaaactcaa tctaacctaa ccaattatgt aatagcaacc tacaccgatt ccatcgatta
    20281 ctatcgacct cgccatgctc gtgtactttg agcgcccatt tccaaccctc ggttgctaac
    20341 gaatactgaa acagaatacc gtctctgttc atgaccaacg cgacagtaca aagctttgtc
    20401 gcaagcaggt tatcgtattc ctaataaccc caactaatgt ttctctccta acctacacct
    20461 gagagtggcg cacgtctgcg ctagtatttt atgccttcta actgctagta gcatcccgac
    20521 tagtcagcca attcatcggc atccaataag atgaattgtt tccgtaggtt ccttaagcca
    20581 ctggcaagga atcgaacctt gcatggatta ctctcattca tcctttattg cgtcttctac
    20641 ttccgccaca gcgacaaatt aatattgtga aaataaatac taagcgtata attttagtta
    20701 tcagcgagtg gtccgctgaa ataaatatac gaaggtgaaa actatgttaa ccaatgaaca
    20761 acgcgctcat gatttagcaa ttgcaacatt accttttgtc agagatattg ttaaatctca
    20821 aataat
//
LOCUS       DAGCYL010000030        20800 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.031, whole genome shotgun sequence.
ACCESSION   DAGCYL010000030 DAGCYL010000000
VERSION     DAGCYL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.031"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            178..405
                     /locus_tag="MB216_000876"
     CDS             178..405
                     /locus_tag="MB216_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBT4525734.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            421..1587
                     /locus_tag="MB216_000877"
     CDS             421..1587
                     /locus_tag="MB216_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBT4525735.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1822..2865
                     /gene="hrcA"
                     /locus_tag="MB216_000878"
     CDS             1822..2865
                     /gene="hrcA"
                     /locus_tag="MB216_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBT4525736.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2959..3522
                     /gene="grpE"
                     /locus_tag="MB216_000879"
     CDS             2959..3522
                     /gene="grpE"
                     /locus_tag="MB216_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBT4525737.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3573..5402
                     /gene="dnaK"
                     /locus_tag="MB216_000880"
     CDS             3573..5402
                     /gene="dnaK"
                     /locus_tag="MB216_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBT4525738.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5553..6719
                     /gene="dnaJ"
                     /locus_tag="MB216_000881"
     CDS             5553..6719
                     /gene="dnaJ"
                     /locus_tag="MB216_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBT4525739.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7087..7824)
                     /locus_tag="MB216_000882"
     CDS             complement(7087..7824)
                     /locus_tag="MB216_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBT4525740.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7826..9328)
                     /locus_tag="MB216_000883"
     CDS             complement(7826..9328)
                     /locus_tag="MB216_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBT4525741.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            9587..11581
                     /gene="uvrB"
                     /locus_tag="MB216_000884"
     CDS             9587..11581
                     /gene="uvrB"
                     /locus_tag="MB216_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBT4525742.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            11591..14407
                     /gene="uvrA"
                     /locus_tag="MB216_000885"
     CDS             11591..14407
                     /gene="uvrA"
                     /locus_tag="MB216_000885"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBT4525743.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            14670..15935
                     /locus_tag="MB216_000886"
     CDS             14670..15935
                     /locus_tag="MB216_000886"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBT4525744.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            15939..16670
                     /locus_tag="MB216_000887"
     CDS             15939..16670
                     /locus_tag="MB216_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBT4525745.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            16805..17689
                     /gene="rapZ"
                     /locus_tag="MB216_000888"
     CDS             16805..17689
                     /gene="rapZ"
                     /locus_tag="MB216_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBT4525746.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            17686..18684
                     /locus_tag="MB216_000889"
     CDS             17686..18684
                     /locus_tag="MB216_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBT4525747.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            18718..19653
                     /gene="whiA"
                     /locus_tag="MB216_000890"
     CDS             18718..19653
                     /gene="whiA"
                     /locus_tag="MB216_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBT4525748.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            20221..>20800
                     /locus_tag="MB216_000891"
     CDS             20221..>20800
                     /locus_tag="MB216_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525749.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEE"
BASE COUNT         6847 a         3577 c         4557 g         5819 t
ORIGIN      
        1 tttttttatg aataaaataa ggacaaaatt gtttaggaag aatcatgagt gtaaaaaaat
       61 catccaagaa gtcgattctt ggatttttta gtggatttgt tcgccaaata cgtttagtat
      121 gcgtatatga gaggttgtaa gcaaagcgtt gtggtctgag cacttaagga ggaatctgtg
      181 aaaatagctt ttcatatttt tgcagatttt gacttaatgc cgaaggtcag ctttgcgatt
      241 accgtttatc gagaggctgt aaaagcaccg tgtagctccg atgacaataa gcaagcattt
      301 ttagagctag gtttccttga ttctaaatat gcggcttatt tcaatgagtt ggtgcttgaa
      361 cgcgatttta agaaggtcga aaacaggcac gacttttcga cgtaaggaga aagaatttca
      421 atgatagaaa caataaatcc cgcagaaaac aaggtttctg cgtacattca tatcccattt
      481 tgcgagcata tctgctatta ctgtgacttt aataaagttt ttttagaagg gcagccagta
      541 gatgagtatg tggaaatgct gctgaatgaa atgagattaa tgctggaaag tcatcctaca
      601 gcgaatttgg aaacacttta tgtaggaggc ggaacaccaa cttctttatc tgctaaacaa
      661 cttgatcgac tattatcagg tgctagagag atcctgcctt ttcaaaatgg taatgagttt
      721 actgtagaag cgaatcctgg tgatttgacc cgtgaaaaac ttcaagtgat gaaaaactat
      781 ggcgtcaatc gtttatccat gggagtgcag acatttgaca atcgcttatt gaaaaaaatt
      841 ggacgtaaac atactgcaga agatgtgtat caaacaatgc aatttttaga agcagaaaat
      901 tttaccaatg taagcatcga tttgatttat gcattaccgg gacaaacact agaaggatat
      961 cgggatactt tggaaaaagc tttggctttg gatctgcctc actattcttt gtactcattg
     1021 atcctcgaaa acaaaacaat gttcatgaac tgggtacgac aaggaagact cgaactgcct
     1081 gatcaggaaa cggaaaccag aatgtttgaa gagacgattg aagcaatgga aaagcatgga
     1141 cgtcaccaat atgaaataag taactttggg ctctccggac atgaatctca acataacctc
     1201 atgtactgga acaatgacca ctattttggg tttggagctg gtgctagcgg ttatcttgga
     1261 gatattcgtt accgcaacaa aggaccaatc caacactatt tacgcccatt acgagcaaat
     1321 gaacgcccgg tattggaaga agaaaaattg acacgaaaaa atcaaataga agaagaaatg
     1381 ttcttaggac taagaaaaaa aacagggatt actaaaaaac atttctatga tcgctatcaa
     1441 aaaacaattg aatcggtgta cggtgatgtg ctaagggaat tagagcaaga agggttactt
     1501 gttaatgaag aagaccgtgt ttatttgaca ccaagaggga cttttttggg gaacaatgtg
     1561 tttgagcgct ttttatttga aaaatagttt gaagaatatt tgttttttat cgaaacttga
     1621 aaagaaaaag tttagaaagt ttgacaatga ggtattgaag tattgatata actggattta
     1681 catcaattat ttagaattag cactcttgtg tttagagtgc taaaaaatgg agtctttctc
     1741 ttgacaggag aagggcttct tgctatattt ataagtgaat tagcacttag aatatatgag
     1801 tgctaatgag aggtgaggaa gatgttaaca aagcgacaaa acgacatttt acgtctgatc
     1861 atccaacact atacgagtag tggcgtgccc gttggttcca aaacattaat gtctgaggga
     1921 gtcaaggcca gctcagcaac gattcgcaat gatatgaaag cattagaaga tgaaggattg
     1981 ctgttgaaga ctcactcttc ttctgggcgg attccatctg ctttaggcta tcgttattac
     2041 gtggatcatt tgttaaaacc tgctcgcgta gcagaagatg atttgctgct gattcgtcag
     2101 tctctaggca gagaatttca tgaaatcaat gagattatta agcaatctgc tgagatatta
     2161 tctgagctga caagctatac gactttttca ttgggacctg aaataaaaga ccgccgactg
     2221 actggctttc gtatcgtacc cttgaataat cgacagatta ttgctatcgt cgtaacagat
     2281 atggggaatg tagaaagcca agtgttcact cttcccgaat atatgggaag tcaggaacta
     2341 gaaaaaatgg tacagattgt aaacgaccgt ctagtcggag atcctctgat gacggtgtac
     2401 cataaacttc gtacggagat tccgatgatt cttcataaat atttccagac gacagaaggg
     2461 atgtctcagt tatttgacac cgttcttggt catgtttttg aagaaaaggt ttatgtcagt
     2521 ggacaaatga atctcttgga ttatgagccc cgtcaagatc ttgatcagtt caaatcgatg
     2581 ttttctttta tgaaagactc agacggactt acacaaatga tcactccgat ggatagcaat
     2641 attcatatca agattggtgc tgaattaggc aatgatttat tgcagaatat gagtatgatt
     2701 caagcaagtt acgagataga tggtcatgga agaggtacaa tagccttgtt agggcctact
     2761 agtatgcctt attctcggat gttcggtcta gtcgatgttt atcgaagaga actagcagct
     2821 aaacttgctg attactaccg ctcgcttgac ttgtcaaact cgtaatgtga ataacgaagc
     2881 tatttgcagc tgatcgttct ctctttctta gtaagagcaa tcaaacagct gactgaataa
     2941 tgaaaggaag actgtatcgt gaaagaaaat caagaagaac tagataaaga aatgacagat
     3001 gctcagccag aaccggaaat cgatgtcgaa gctgcagaag attcgggtat ttcagaagct
     3061 gaagcggaag aattcgaaac agcgaaatta aaagctgagt tagaagaaat ggaagataaa
     3121 tacttgcggg ctcgtgctga aatcgcaaat atggcaaatc gaggaaaaaa tgagcgtgag
     3181 caattacaaa aataccgttc acaagattta gccaaaaaat tacttccatc aattgacaac
     3241 ctggaacgtg cattagccac agaagtgtca gatgatcaag gggcaggttt gaaaaaaggc
     3301 gtggaaatgg tcttggaaag tttgcgtaac gcactggaag aagaaggaat tgaaaaaatc
     3361 cctgctaaag gagaagcctt tgatcctaat cttcatcaag cagtacaaac tgttccagcg
     3421 actgaagata caccagccga tacgattgta gaagtactac aagaaggcta caaattacat
     3481 gatcgcgtgt taagaccaac tatggtcatt gttgcacaat aattgtgcgg atataaaact
     3541 tacactttca attaaagaag gagacgttaa ttatgagtaa aattatcggt attgacttag
     3601 gtacgactaa ctctgcagta gcagtattgg aaggaaacga agcaaaaatc atcgctaacc
     3661 cagaaggaaa ccgtacaaca ccttctgttg tatcattcaa aaatggagaa atccaagtag
     3721 gggaagttgc aaaacgtcaa gctgtgacaa acccaaatac gatttcatca atcaaacgcc
     3781 atatcggtga agcaggttac aaagtagatg tagaaggtaa atcatataca ccacaagaaa
     3841 tctcagcaat gatccttcaa tatatcaaag gattcgcaga agactattta ggtgaaaaag
     3901 tcgaaaaagc agttatcact gtgccagctt actttaatga tgcacaacgt caagcaacaa
     3961 aagatgctgg taagatcgct ggtctagaag tagaacgtat cgtcaatgaa ccaactgcag
     4021 ctgctcttgc ctatggttta gataaaacag atcgcgacga aaaaatctta gtattcgacc
     4081 ttggtggcgg tacttttgac gtatctatcc ttgaactagg agacggtgtc tttgatgtat
     4141 tgtcaacagc tggcgacaac catctaggtg gggacgactt tgataacaaa atcatcgact
     4201 acatggtagc agaattcaaa aaagaaaaca gcattgactt gtctcaagac aaaatggctt
     4261 tacaacgctt gaaagacgct gctgaaaaag cgaaaaaaga tttatctggc gtatcaagca
     4321 cacaaatcag cttgccattt atcacagcag gagaatctgg accattgcat ttggaaatga
     4381 cattgacacg tgcaaaattt gatgaattaa cagctgactt agtagaacgt acaaaagtac
     4441 cagtacgtca agcgttaaaa gatgcaggtc tgtcacaatc agaaatcgac gaagttattt
     4501 tagtcggtgg atctactcgt atccctgcag ttgtagaagc agtacgtaaa gaaacaggaa
     4561 aagaaccaaa caaatcagtt aaccctgatg aagtagttgc tatgggtgct gctatccaag
     4621 gtggcgtgat cactggtgat gtgaaagatg ttgttttact tgacgttaca ccattgtcat
     4681 taggtatcga aacaatgggt ggagtgttca ctaaattgat tgaccgcaat acaacgattc
     4741 caacaagtaa atcacaagta ttctcaactg cggcagataa tcaaccagca gtagacatcc
     4801 atgtattaca aggtgaacgc ccaatggctg ctgacaacaa aactctagga agattccaat
     4861 tgacagatat tccagcagct cctcgtggta ttccacaaat cgaagtaaca ttcgatattg
     4921 ataaaaacgg gattgtaaat gtatctgcga aagatctagg tacacaaaaa gaacaaaaaa
     4981 tcacgatcaa atcttcttca ggtctaacag acgaagaaat cgaacgtatg gtgaaagatg
     5041 cagaagcaaa cgctgaagca gataaagctc gtaaagagga agttgaccta cgtaatgatg
     5101 tagacgcatt gctattctct gtggacaaaa cattgaaaga attagaaggc aaagtggacg
     5161 aagaagaagt gaaaaaagca gaaacagctc gtgacgaatt aaaagccgct gttgaagcaa
     5221 acaacatcga ggaaatgaaa actaaacgcg atgcattgaa cgaaatcgtt caaaacttga
     5281 ctgttaaact atacgaacaa gctgcacaac aacaagctca agaaaatcca gaagcagctc
     5341 aaggcggagc agatgatgtc gttgatgctg attttgaaga agtcgatggc gacgataaat
     5401 aatcagttca ctgaatttcg agagaaggcc aaagttgcta ctttggcttt ttcttttgaa
     5461 aaatagtttg aagtatcggt ttttgtttca aacaaaatat gatatgataa caagcgacgt
     5521 gaaaatgatg agaaccaata ggaggaaagc ctatggcaac aaaacgtgat tattatgaag
     5581 tcttagggct ctcaaaagga gcatcagaag atgaaataaa gaaagcttat cgtaagcttt
     5641 cgaaaaaata ccatcctgac atcaataaag aacctgatgc ggaagaaaaa ttcaaagaag
     5701 tatcagaagc atatgagatt ttaagtgacc cgcaaaaacg cgctgcatac gaccaatacg
     5761 ggcatgcagg cacagatcca aattacggtg ctggtggagc tggtggcgga tttggtggat
     5821 tcggcggctt ctctggtggc ggcttcggtg gttttgaaga tatcttcgac tctttctttg
     5881 gaggaggcgg tcggtcagtc gatccaaatg cgcctcgtca aggtgctgat ttgcaatata
     5941 cggtcgactt gtcttttgaa gaagcgattt ttggtgtaga aaaagaaatc aagtataatc
     6001 gggaagaaat ctgtcatact tgtggtggaa acggtgcaaa accaggcaca caaccaacta
     6061 cttgtcataa atgtcatggt gcaggtacga tcaatgtaga acgccaaacg ccacttggac
     6121 gagtgatgag ccgtcaaact tgtgatgttt gtcatggcac agggaaagaa atcaaagaac
     6181 cttgtccaac ctgtcatggc tcaggacatg agaagaaagc acattcagtt aaagtgaatg
     6241 taccagctgg tgtagaagaa ggacaacaaa tgcgtttagc tggtcaagga gaagctggag
     6301 aaaacggcgg accatttggt gatttgtatg tggtttttcg tgtagaagaa agtgatatct
     6361 ttgatcgtga aggttctgaa atttactatg aattgccttt aagttttgta caagcagctt
     6421 tgggggatga agtccaagta ccaactgtcc atggagatgt gaaattgaag atccctgctg
     6481 gaacacagac aggtacgaac ttccgtttac gtggaaaagg cgctccgaag ttacgtggtg
     6541 gtgcaacagg cgaccaacat gtcaaagtaa aactgatcac accgaaaaat ctaaatgaac
     6601 aacaaagaga agcattacgt gcattcgctg aagctggcgg catcaaagtt gaagaacagc
     6661 aagaagacgg tttcttcgat aagtttaaag atgcttttgg cgggaaaaag cggaagtagt
     6721 ttttctagta aaaatataaa gtagacttgc ccaaattttc tatttctagt aaaaacgcta
     6781 agtcattaat aattaaaaac cagacgaaca atcatacgtt cgcctggttt ttttgtagtt
     6841 gtcatgtttg taccaacaaa aaagccagct ttagaataag ataatcatca tagtttctga
     6901 aggtggtatc gactatgtac ttgatatcgt caatgaaccg atggtacttt cttatgcgat
     6961 ggaacaggta gagcgattgc ttgtttgtgc aggagaacaa gcgatgcggc tggtacagtt
     7021 aagtgaaaaa acaagaaact aaaaatgccc ggtcgtcatg aagcgaccgg gcatttttgg
     7081 tttttattaa atatttaata ctttattcaa gaaatctttt gtccgttcat tttgcggatt
     7141 ttcaaagatt tgttgaggcg ttccgtcttc taaaaagttt ccgtcgtcaa tgaacatgac
     7201 acgattggcc acttctttag cgaagcccat ttcgtgcgta acgataacca tcgtcatacc
     7261 ttgttctgct aactttttca tgacgttcaa gacatcaccg accatttcgg ggtcgagtgc
     7321 agatgtaggc tcatcaaaaa gcatgatgtc agggttcatc gctagagcac gagcaatagc
     7381 cacacgctgc ttttgtccac cggaaagcat ttcaggatac atatcttttt tatcttctag
     7441 accaactgtc tgcagcaatt tgattgcttt tttctctgct tcttctctag attctttctt
     7501 caggtccatc ggtgctaaaa tgatgttttc caaaatagaa agatgaggga ataggttaaa
     7561 gtgctggaat accattccga tatgttgtct caccaagttg atatctgtgc ttttatccat
     7621 caagtgggcg ccgtcaataa taatttcacc gtcagaaggt tcttctaaac gattcaagca
     7681 acgcaaaaat gtactttttc ctgaacctga agggccaatg atacagacga catctccttc
     7741 gttgatggac acattgatgt cgtttaatac tgtgttatcg ccgtattttt tgactaaatg
     7801 ctcaactaag attttttctg ccatcttact tcaccttctt ttctaaaact tttgctaatt
     7861 ttgtcaatgc tgtgatcaaa atcaagtaca tgatcgcaat gatcaagtaa acatacgtac
     7921 tttgtgttgt acgtgcaaca atgatcttac cagtttgcag caattcgact acaccgattg
     7981 cggaaatgat cgttgtgtct tttaatgaga taacgaactg attgacgaat gatgggatca
     8041 tgatgcgtat cgcttgcggc aaaatgattt tttgcattgt ccgattataa gaaagaccta
     8101 aacttctgga agcttccatt tgtccgacag gaacggcgtt gatcccgcct cgaacaatct
     8161 ctgcgatata agcacttgcg ttaagagtca aggtgataac accagcaagg aagtcaggaa
     8221 tcgtaaatcc aacgatacca ggcaacccaa agaaaataaa gaacgcaaga accatcattg
     8281 gaattcctcg gatgatatcg acataaatac tagcaattgt gcgaagagcg cgtattggtg
     8341 ctacactgaa taatccaaaa atcacaccga ttactatagc caaagcaaag gagatcaaag
     8401 ttaggagaat tgttctccaa agtcctttca acaatgcttg atagttgttt tggataagtc
     8461 caagaaatgt tgattcatca gcagtggaag actcactgga agtttctgaa ccatccgcta
     8521 catattcttt aataatctta tcgtattcgc ctgttctttt taattctttc aatccttcat
     8581 tgaacatatt aagaagctca ggattttgtc cttttttaac ggcaaatgca tattttccgc
     8641 cagattcttt tttgatcggt gtttccaatt cttgtccttg tttgatcgca taaccgatta
     8701 ccggataatc atccatcatt gcatcgattg cgccaacttt taacccgtcg tacaattgat
     8761 cagcagcatc aaaatatttg atagaatagc catattcaga ttcatggtcg cttaaaaatt
     8821 cagcactttc ggtaccaact tttgctccga ctgtttttcc tttcaaatct tcataagact
     8881 tgatcgaagt atctcctttt ttgacagcta attggatccc gctatcaaag taagaatcag
     8941 aaaaatcata gcttttcttt cgttcatctg ttacagtcat tccggcaatc attccgtctg
     9001 cttgtcctga ttccaaagct tgcaaagcag cagagaaacc acggtagtcc attcggatgt
     9061 taaatccttg cagtttggcg atgttttcca tcaattttac atcgatgcct tcgtactcgc
     9121 cctttgcgtt ttgaaattca aatggagcaa aagcagagtc actggcaatt acgtatacgt
     9181 cttttttggg ctctattttt tgcattgtct cgcttgtgct tgactctgcc gttgtagtgt
     9241 tgtcagcttg tgcaacatgt ccaaacgcaa gtgtcacaag tcctgtcatt aagaaaacaa
     9301 atgagtgaaa taaatgcttt cttttcataa tcaacctcct aaatttccaa tattttatta
     9361 ttataggcga aaagtgctga atcagacaag actgtaagct cgaaaaaatg aaaaaaatgc
     9421 gagtttctaa cattttatgt tataaaacgt aacatgcata ataagataaa aaagtggcga
     9481 aagtatgttc ggtgtggtat aattttttag taaggaatgc ctttgctttc cttttctttt
     9541 tattggtcgt aaaatgaaaa ggaatgaatg aaaaggagga cgaagaatga ttgagagaga
     9601 tacctcccga cactttgagc ttgtctcgaa ataccaaccg gctggtgatc agcctgaagc
     9661 gattaaccaa ttagtggatg gcgtagtcgg cgggaaaaaa gcacaaatct tattaggtgc
     9721 gactggaaca gggaaaacat atacgatttc caatcttatt gagaaagtga ataaaccaac
     9781 actaattatt gcccataaca aaacattggc tggtcagctg tacggggaat tcaaggaatt
     9841 tttccctaac aatgctgttg aatattttgt ttcttattat gattattatc agcctgaagc
     9901 ttatgtgcct tcaagcgata cgtatatcga aaaagattca agtgtaaatg atgagatcga
     9961 caaattacga cattcggcta ctagttcatt attagaaaga aatgatgtga tcgtcattgc
    10021 atccgtttca tgtatttttg gattaggttc gccttttgag taccaaaaac aagtagtttc
    10081 gattcgacag ggagcagaac tagatcgcaa tcagttgatt cgtgacttag tcagtatcca
    10141 attcgaaaga aatgatatag attttcagcg cggtcgtttt cgagtgcgcg gtgatgtcgt
    10201 ggagattttt ccggcttcaa gagatgaacg tgccttacgt gtggagtttt ttggagatga
    10261 ggtcgaacgt atccgagaag tcaatgcact gacaggtgaa gttttaggag aaacggaaca
    10321 cgtggcaatc ttcccggcga ctcactttgt gacgaatgat gaacatatgg agcatgccgt
    10381 agcgaatatc aaagcggaat tagaacagcg tttaactgtc ttgcgaaatg aaaacaaact
    10441 tctggaagct caacgtttag aacagcgaac caattacgat attgaaatga tgttagaaat
    10501 gggctacacg tcgggaattg aaaattattc tcggcatatg gacggacgta aagaaggtga
    10561 accgccttat acgctattgg atttctttcc tgaagatttt ttgatcgtgg cggacgaatc
    10621 gcacgtaacc atgcctcaag tcagaggaat gtataatggt gaccgagcga gaaaacagat
    10681 gctagtagat tacggtttcc gtttgccatc tgctttagac aatcggccac ttcggttaga
    10741 agagtttgaa aaacatgtca atcagatcat ttatgtctct gcgacgccag gaccatatga
    10801 acatgaacaa acagataccg ttatccaaca aatcatccgt ccgacagggc tgcttgaccc
    10861 agtgatcgaa gttcgtccaa tcatgggaca aatcgatgat ctagtaggcg aaataaatga
    10921 acgagtcgaa aaagaccagc gggtgtttgt aacgacattg acgaaaaaaa tggcagaaga
    10981 cctcacagat tatttcaaag aattagggat caaagtcaaa tatttgcata gtgatatcaa
    11041 aacgctggaa cgaacagaaa tcattcgaga tcttcgatta ggtgaatttg atgtgttagt
    11101 tggaatcaat ttgcttcgtg aaggattaga cgtacctgaa gtttcccttg ttgctatctt
    11161 ggatgcggat aaagaaggct ttttacgtag tgaacgttcc ttagtccaaa cgattggacg
    11221 agctgctcgg aatgaagaag gtaaagtaat catgtacgca gacaaagtaa cagattctat
    11281 gcgtctagcg atggatgaaa catcaagacg ccgtacgatt cagcagaagt acaatgaaga
    11341 acatgggatc gttccaaaaa cgatcattaa agaaatccga gatttgattt ctattacgaa
    11401 agaatcggaa gatgatacaa aagaagcggt acaagtctcg tatgaagaaa tgacgaaaga
    11461 agaaaaagat acgttattga tgaaattaga aaaagaaatg aaaaatgcgg cgaaagcatt
    11521 ggattttgaa acagccgcta atgtgcgaga tatgatttta gaattaaaag cttcaaagta
    11581 ggaggacaac atggctaacg ataaaattac gattcacggt gcacgtgccc ataacttgaa
    11641 aaatatagat gtaacgattc cacgagataa attcgtggtc gttacaggtt tgtccggttc
    11701 tggaaaaagt tccctagcat ttgatacgct gtacgctgaa gggcagcgga gatacgtaga
    11761 gagcctttct gcttatgctc gtcagttttt aggacaaatg gataagccgg acgtagatag
    11821 catcgacgga ttgagtccgg cgatttctat tgatcagaaa acaacaagta aaaatcctcg
    11881 gtcgacagta ggtactgtga cggaaatcaa tgactattta cgcttgttgt ttgcacgtgt
    11941 aggacatccg atttgtccca acgatcatat cgagattacc agtcaatcag tagatcaaat
    12001 ggcagatcaa gtaatggctt tgcctgagcg cacaaagatc caagtgttgg caccgatcgt
    12061 ggtaaagaaa aaaggacaac ataaaaaaat attcgaacga atccaaaaag agggatacgt
    12121 ccgcgttcga gtcgatggtg agatctatga tttaagtgaa gtgcctgagc tggagaaaaa
    12181 caaaaaacat gatatcgcga tcgtcatcga ccggatcatt gtgaaagaag ggattcgttc
    12241 tcgtctattt gattctttag aggcggctct gcgattggca gacggctatg ctctggttga
    12301 cgtgatcggt gaagaagaaa tgttgtttag cgaacactat gcctgtcctt attgcggatt
    12361 tactgttggc gaactagaac caagactgtt ctcattcaat gcgccttttg gagcttgtcc
    12421 agactgtgat ggtttaggtg tcaaattaga agtcgatcaa gatttagtga ttccagatag
    12481 aacgaagaca cttagtcaag gtgctatcgt tccatggaat ccaatcagct ctcagtatta
    12541 tcctcaaatg ttagcgcagg cttgtgaaag tttcggtatc gatttagata caccattcga
    12601 agaattaccg gaagagcatc aggatatcat cttgaatggg acagatgatc ttttccattt
    12661 ccattatgaa aatgattttg gtggcgttcg cgatgtagaa acgactttcg aaggggtatt
    12721 gaagaatatc gagcgtcgtt atcatgatac gaacagtgat ttcacgcgtg agcaaatgcg
    12781 tctttatatg acagaattaa cttgtcagac atgtaaaggt tatcgattga atccccaagc
    12841 tctatctgtt caaatcaatg gaacgaatat cgggcaaaca aatgagttgt ctattcaaaa
    12901 agcagtagat ttcttctcaa acttaacgtt atctgaacaa gagaaagtaa ttgccaaacc
    12961 gattttaaaa gaagtaaatg acagactttc attcttggaa aatgtgggac tagactatct
    13021 aactttgagt cgcgcttccg gaacattatc tgggggagaa gcgcagcgaa tcagattggc
    13081 tacacaaatt ggatcgaatc tttctggagt tttgtatatt ttagatgaac catcgatcgg
    13141 attgcatcaa cgcgacaacg atcgactgat tggctcatta aagaaaatgc gtgatcttgg
    13201 caacaccctc gttgtagtcg aacatgatga agatacgatg tgggcagcag attatctgat
    13261 tgacgtcgga cctggggccg gagaacaagg cggacaaatc gtggctgcag gcactcctga
    13321 agaagtagct aatgatgaaa attctttgac agggaaatat ctttcaggga aaaaatcaat
    13381 ccctgtacca aaagaacgcc gtaaaggtaa tgggaaagca atcaagatca ctggtgcttc
    13441 agaaaacaat ctgaaaaata ttagtgttga atttccatta ggagaatttg tagcagtcac
    13501 aggtgtgtct ggttctggaa agagtacatt ggtcaatagt atcttaaaga aatcgttagc
    13561 gcaaaaatta aataagaatt ctgctaagcc aggtaaattc aagacaattt ccggctacga
    13621 aagtatcgaa aagatcatcg atatcgatca aagcccaatc ggccggacgc cgagaagtaa
    13681 tccagcgact tatacaagtg tatttgatga tatccgtggg ttatttgctc aaacgaacga
    13741 ggcaaaaatg cggggttata agaaagggcg ttttagtttc aacgtaaaag gcggtcgttg
    13801 tgaagcttgt cgcggggatg gaattattaa gatcgaaatg cactttttgc ctgatgtcta
    13861 tgttccttgt gaagtatgtc atggcaaacg atataactct gaaacattag aagtgcatta
    13921 caaaggaaaa agcattgctg atattttgga aatgacagta gaagatgctg tagaattctt
    13981 caagcacatt ccaaagattc atcgcaaact gcaaacgatt gttgatgttg gcttaggtta
    14041 tgtgacgatg gggcaaccag caacgacatt gtccggtggt gaggcacaac ggatgaaact
    14101 tgccagtgaa ttgcacaaaa tctctaatgg aaagaatttc tatatactag atgaaccaac
    14161 gacaggactt catagcgatg acatcgcccg cttgttgcat gtattacaaa gattagtaga
    14221 tgctggtaac acagttttag tgattgaaca caatctagat gtaatcaaaa cagcagatta
    14281 tatcattgat ttaggaccag aaggtggaga aggtggagga acgatccttg cgactggaac
    14341 accagaagaa atcattaacg taaaagaaag ttatacaggt cactatttga aaaaaataat
    14401 ggtataaaga ggatagatag ctgagatagt taaaaaaaca taaaaaaatg aatttttgct
    14461 tgttttatta atatcagtgt tctagaatca ttacatggta gttctttcta cactgattgg
    14521 tatagacaaa aacttcctat aagttcaatc cgttcaaaaa agaattgagc aatcggagga
    14581 gtttcctttg attgcttttt tctttatggt atgatattcc ttgtgtagtt ggaaaagaaa
    14641 aacaagagat tatgagtaag tgagagataa tgatgaacag tattgaaaat gaagaattta
    14701 tccctatatt attaggaagc gacatgaacg tttatggtat ggcacgttct tttaatgaag
    14761 catacggcaa aatctgccaa gcatatgctt cggatcagtt agcccctacc cgctacagta
    14821 aaattgtcaa tgtagaagtt attccaggat ttgataaaga ccctgtgttt atcgagacga
    14881 tgcttcgttt ggcaaaagaa agatattctg ataagagtaa aaaatatctt ttgattgctt
    14941 gtggtgatgg atatgcagaa ttgatctccc aacataaaca agagctgtca gaatatttta
    15001 tctgtccata tattgattat tctttatttg aaagactgat caataaagta agcttttatg
    15061 aagtttgtga agaatatgat cttccgtatc ctaaaacatt gatcgtcaga gaagaaatgt
    15121 tagtaaatgg acatttggaa caggaattgc cttttgaatt cccagttgct ttgaaacctg
    15181 caaacagtgt ggagtatctt tctgtccaat ttgaaggaag aaaaaaagct tttattttag
    15241 agactagaga agagttcgat ttaattttag gccggatata cgaagccggt tataaaagtg
    15301 aaatgattgt acaagatttt atcccgggtg atgatagtaa tatgcgtgtg ctaaatgcgt
    15361 atgtagatga agatcatcaa gttcggatga tgtgtcttgg tcatccactg ttagaagatc
    15421 ctacaccagc atcgattggt aattacgttg tgatcatgcc agattataac gaaaaaatct
    15481 atcaaaccat taaggcattt ttggaaaaaa tcgaatatac aggctttgcc aatttcgata
    15541 tgaagtatga tcctagagat ggagaatata aattatttga aatcaatttg agacaaggtc
    15601 gtagcagttt ctttgtgaca ttgaacggct tgaatctggc tcgtttcgtc acagaagatc
    15661 gagtgtttaa caaacccttt gttgaaacga cctatggaac gaatcaatca gataaagcaa
    15721 gattatggat gggtgtgccc aaaaaaatct ttttagaata tgctcgggaa aatgaagaca
    15781 aaaaactagc tgagcaaatg atcaaagaaa atcgttacgg tactactgtg ttttatgaaa
    15841 aagaccggtc aatcaagcgc tggttgttga tgaaatatat gttccataac tatattccaa
    15901 gattcaaaaa gtattttcat gtgaaagaag gctagttcat ggagaatttt ttcagtattt
    15961 taggcggaat gggcacgatg gcgacagaaa gttttgttcg cttgatcaac caccgaacga
    16021 aagctacaaa agatcaagaa tatttgaatt atgtcttatt caatcatgca acagttcctg
    16081 atcgtacagc ttatatttta gatcgatcag aagaaaatcc aatgccgttt ctgctggatg
    16141 atattgagaa acaaaattta ttgcagccga acttcattgt gttgacttgt aacaccgcac
    16201 actacttttt tgaagaatta caagcggcaa cggatattcc tatccttcat atgccaagag
    16261 aagcagcaaa tgaacttgtc cgtcagcata cgacagggag agtagcgatt ctaggaacag
    16321 aaggaagtat gaaagctgga atctatgaac gcgaagtcaa aaatcttgga ttcgaaacag
    16381 tgattcctga tactgttctt caagaaaaaa tcaattatct gatctatcat gagatcaagg
    16441 aatcggatta tttgaatcaa gagctatatt acgaaatctt agaagaagcg gtagagcgcc
    16501 taaactgtga aaaagttatc ttaggttgca cagaattgtc attgatgcat gaatttgctg
    16561 aagataacca ttatccagtg atcgatgccc aatctatttt agcagatcgg acgattgaac
    16621 gagcgctagc tgaaagaagc aaggcattgg atacagcatc ggaaaagtaa aaaaattgga
    16681 tttggggcaa atatcctaaa agatatttgg tccaaatctt tttgtaaaag aaaaagaaaa
    16741 ctggtaatga tacgcccgga atgatataat tttactatgc acaatgtgag aggagttcgt
    16801 aattatgaca gataatttgc aattagtcat cataacaggt atgagcggtg cagggaaaac
    16861 agtagctatc caaagttttg aagatatggg ctatttttgt atcgataata tgccgcctcg
    16921 attgattcca aaattctggg aattaatcaa agaatcaggc aaagtcacaa aaatggcttt
    16981 aggagtggat ttacgttctc gttccttctt tgaagaaatc caaaacatgc tgatcgaaat
    17041 cgaaaatacg aacttcatcg acacttctat cttattttta gatgcttcag acgaagaact
    17101 agtggctcgc tacaaagaaa caagacgagc acatccaatg gcgatggacg gtttgatcac
    17161 tgaagggata cgtaaagaac gggcgatact agacgacttg aaagcccaag cttccgtgat
    17221 cattgatacg acgacattga caccacgaca attacgtgaa aaaatcaatg agtcattcaa
    17281 atcaaaggga gaccaaggat ttcggatcga atttgtatca tttggtttta aatacgggtt
    17341 gccgatcgat tcggatattg tgatggatgt gcgttttttg cccaaccctc attatatccc
    17401 ggaactaaga ccattgaccg gaaaagatca gccagtctat gattatgtga tgtcctttga
    17461 tgaaacgcaa tcattctacc ataaattttt ggatcttctg gaaactgtat tgcctggata
    17521 cattaaagaa ggaaaaagca gcttgacagt tgcaatcggc tgtacaggcg gtcaacaccg
    17581 ttctgtggca ttgactgaac gtttggcaaa tgctttaggg aaaaactata aagtcaatgt
    17641 gacacatcgg gacaaggata aaaggaaaga gacggtgaac cggtcgtgaa aatgaaaacc
    17701 taccggataa gaaaaccaaa gatcgtagtg gtcggcggtg gcactggtct gccagttatc
    17761 ctgaaaagtt tgcgaaatca aggagcagat atcacagcag tagtgactgt tgcagatgat
    17821 gggggaagca gcggtgcaat cagagaatcg atcgctatgg ctcctcctgg tgacttgaga
    17881 aatgtcttag tggcgttatc ggatatgcca caattctatg aagatatttt ccaataccgt
    17941 ttcaaaaagg aagatcagtt tttagccaat catacgatcg gtaatctgat cattgccgca
    18001 gtttccgaga tgcggggaag cacttatgag gcgattcaat tattagcaaa aatgatgcat
    18061 gtagatggac atgtttatcc ttcttcagaa actcctttga ccttgcatgc tgtctttaaa
    18121 gacggaacga ctgcagtagg agaatctaaa atcgcgatcg accgtaaaac cattgaccgt
    18181 gtgtatgtct gcaacacaaa tgataaaagt gaagccaaag cggctcgaaa agtcgtttca
    18241 gcaattatgg acgccgacat ggtcgttttg ggacccggaa gtttattcac cagtatcctt
    18301 ccaaatttag tgataccaga aatcggcgaa gcaatgctca agacaactgc tgaaaccgtc
    18361 tatatctgta atatcatgac ccaaaaaggg gaaacggaac atttcacaga tgccgatcat
    18421 atacgtgttt tacacaagca tttgaatcag ccttttatcg atacagtatt ggtcaatacg
    18481 gaaaaagtgc ctgaagatta tatggaccca gagatctatg atgagtatct agtacaagtc
    18541 gcacacgact tcaacggatt gagaaatgaa ggatgtcgag tgatttccac cgactttttg
    18601 gaattgaaag atggtggtgt cttccacgat ggagacaaag tagtagaaga attgttccgt
    18661 ttagttttcg gttcaaaata ttaagcaatt tattgttgga gaaaggagga gaataggatg
    18721 tcatttgctg cagatgtgaa gaaagaacta actggtttgg aagttcatcg agaacatgcg
    18781 aaagcggaat tagctgcttt gctgcgaatg aacggctctt taggtattgt gaaccagcag
    18841 tttattttga acgtccaaac agaaaatgct gcaattgcca gacgtatcta ttctcttttg
    18901 aaagatcatt atcaggtacg aagtgagcta ctcgttcgta aaaaaatgaa attaaaaaag
    18961 aacaatgtct atatcgtccg gttgaagcaa ggcacaaaag atgtgttgat ggatcttgat
    19021 atcatggatg gtatgatgtt caatggacac gtttcggacg agattatggg aaattcgcaa
    19081 aaaatgcgtt cttacttacg tggtgctttt atggcctcag gttcagtcaa taatccggaa
    19141 acgagccgtt atcatttgga aatattttct atttatgaac agcataacca agacatttgc
    19201 gatatgttaa attattacgg tctacatgct cgaacgcttg aacggcgcaa tggctatatt
    19261 tcttatttaa aaggagcaga aaaaattgct gatttcttaa cattgattgg tgcaaccaac
    19321 tcaatgctcc gatttgaaga tgttcgaatc gtacgggata tgagaaattc ggtcaatcgt
    19381 ctagtcaatt gtgagacagc aaatctgaac aagacgattg atgcggcttc taaacaaatc
    19441 gaaaacatcg aattgatcga agcaaaggtc ggtctacatg cccttcctga aaaacttcag
    19501 gaaatcgcag aacttcggct tcaacatcca gaggtcagct tgaaagaatt aggggaaatg
    19561 attccctcag gtgcgatatc aaaatcggga attaatcacc gaattcgcaa aataaatgaa
    19621 tttgcggaaa aactgcgtga agaagcagtt taatccaaag aaaaaactga tacatgaagc
    19681 aggagatgcg aatgtatcag ttttttttaa aagttatact tgcttttctt ccgtcaaact
    19741 ataattccat ctttactgga gtaacataat ttggaaaggc ttctattgtt tttaatacaa
    19801 tcgtataaaa agcggggaga caactgaatg aagattttag ttttgtacat aaatttttgc
    19861 ttaatataga agtcctgata tagataagat caagtattta tctataactg gcctgattaa
    19921 ttaaaaaact ggaaggaact gctcgatcat ccttgagaac tgctcttctt acttctggtc
    19981 aagattctcg tggttggtgg ataaggatgg ttgataagtt cattggatta gctagtcgaa
    20041 aacaaccagg tgtaattaat gtagaagttt taacaagggg atggagtgat ttattcagtg
    20101 ataatcataa gttcataaat agtaattgag atgtatggtt ggaatacgtt atacctgaac
    20161 ttgaactacc aagatttggt ttagaatttg agaatcgatg tttataaagg gagggcaaaa
    20221 atgcgttttt ttacaacttt tactatcatt atgattgcag tactttttat ttttctagat
    20281 atagcaaagc gaaatacagc atttttatta tatcgggttt tacttcgagc aggtctcata
    20341 acatttatat cgatcgttgg attttttcta ttcacagtta ttgtatttat ttggagaact
    20401 ccagcaccac cattgccaga gattacatat ggagaatttc cttttagact tgagtatgaa
    20461 ttgaatgaag aattacatgt gatagaagac acgttaatag ttgaatttga tggatttgga
    20521 atgaacgaag gcatagggag atatcgtaga tggacaagtc gtttagccag cggagaagat
    20581 ttagtgttgc tgttagaagt tagtgataat aagcaaatat tttattttcc aggacctgct
    20641 aattattaca tgggagatag gttgaatggt tataatcata cttttcctag tgcttctttc
    20701 attgaaaggg aaagaggcat tactagaagg gatattttac atgacaaaga attattagaa
    20761 caatttggtc cgcttgatca aaatacaata aatgaagaag
//
LOCUS       DAGCYL010000031        20474 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.032, whole genome shotgun sequence.
ACCESSION   DAGCYL010000031 DAGCYL010000000
VERSION     DAGCYL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.032"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(80..289)
                     /locus_tag="MB216_000892"
     CDS             complement(80..289)
                     /locus_tag="MB216_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525750.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(300..1628)
                     /locus_tag="MB216_000893"
     CDS             complement(300..1628)
                     /locus_tag="MB216_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525751.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            1764..1919
                     /locus_tag="MB216_000894"
     CDS             1764..1919
                     /locus_tag="MB216_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525752.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(1993..2580)
                     /locus_tag="MB216_000895"
     CDS             complement(1993..2580)
                     /locus_tag="MB216_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBT4525753.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(2663..3913)
                     /gene="clpX"
                     /locus_tag="MB216_000896"
     CDS             complement(2663..3913)
                     /gene="clpX"
                     /locus_tag="MB216_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBT4525754.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(4160..5449)
                     /locus_tag="MB216_000897"
     CDS             complement(4160..5449)
                     /locus_tag="MB216_000897"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBT4525755.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(5603..6535)
                     /locus_tag="MB216_000898"
     CDS             complement(5603..6535)
                     /locus_tag="MB216_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525756.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(6692..7351)
                     /locus_tag="MB216_000899"
     CDS             complement(6692..7351)
                     /locus_tag="MB216_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBT4525757.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(7354..8379)
                     /gene="sppA"
                     /locus_tag="MB216_000900"
     CDS             complement(7354..8379)
                     /gene="sppA"
                     /locus_tag="MB216_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBT4525758.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(8392..8682)
                     /locus_tag="MB216_000901"
     CDS             complement(8392..8682)
                     /locus_tag="MB216_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525759.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            9092..10897
                     /gene="glmS"
                     /locus_tag="MB216_000902"
     CDS             9092..10897
                     /gene="glmS"
                     /locus_tag="MB216_000902"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBT4525760.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(10969..12324)
                     /locus_tag="MB216_000903"
     CDS             complement(10969..12324)
                     /locus_tag="MB216_000903"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBT4525761.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12348..13517)
                     /locus_tag="MB216_000904"
     CDS             complement(12348..13517)
                     /locus_tag="MB216_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525762.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(13514..14401)
                     /locus_tag="MB216_000905"
     CDS             complement(13514..14401)
                     /locus_tag="MB216_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBT4525763.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(14590..18270)
                     /gene="nifJ"
                     /locus_tag="MB216_000906"
     CDS             complement(14590..18270)
                     /gene="nifJ"
                     /locus_tag="MB216_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBT4525764.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            18468..18989
                     /locus_tag="MB216_000907"
     CDS             18468..18989
                     /locus_tag="MB216_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBT4525765.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(19688..20253)
                     /locus_tag="MB216_000908"
                     /pseudo
     CDS             complement(19688..20253)
                     /locus_tag="MB216_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5770 a         4419 c         3549 g         6736 t
ORIGIN      
        1 attataaatc agtcaaaaaa aaataaaaaa ccagtcaaaa agactggttt tttatttttt
       61 tgtctatcat caagccgttt tatcgttttt tattttcgct tcttgttttt ttcatataaa
      121 cagaagcagg ttttctttcc ttatgaagaa gataataata aagtcccatc ccgccaaaca
      181 tgaccaaaaa accgatgact gatcgaatag gaaaattagg aacaaaaaag agacagacgg
      241 ataataatga tgccggaacc attattcgat aatagagttg tttatccatt ttcttcacct
      301 catatttttt gtaaagtaat caattgttct aattctcggt aatctaaaaa ttgcacacgc
      361 agatgctgct cacctttctt gacatcgaat tggatacttc gccgcttttt ctttattcgt
      421 ttgaaggtaa catcagagag tctatgtttg atttccttta acggtaattc tgacagggat
      481 tctgaaggaa catagattct taaggataaa agatactctt gatatttttc atctgttacc
      541 tctacttcta gatattccct aatataataa caataagctt cgattccatt atccaaagca
      601 acagtcggat cgataacttt atagcacatc atgcgcgagc gattctcgtc aacccaagga
      661 aaatacgcct gagcatttag tgttccgcga acacgccttt ctttataatc tccttgttga
      721 atatcataac tttttgttac aaaaaatgaa gcattctttt taggaacagg ttgcatttgt
      781 gcattctgat actggacaga ttggatcgta tggtcagtgt aaatgtattc aacgaatggc
      841 ggtttcaata aaccgacacc attgtcccaa gcaagatgat caaacccaag ctggtcccta
      901 aaataggatt gaaacatttg attgtttggc ttttgccagt taaaggggtc tcgataaagc
      961 tggatacata tgacttcgtt ttctaattcc ttcacttttt ctacttgttg gatgtcgaga
     1021 aaaatcggtt tagggatgat ttgatgataa tagcgactat aatacatttc ccaacacgaa
     1081 gtgaaacata aaccttggta aaaaagatcg tatcctggaa attggttgca atcgactact
     1141 acttccccgt tttgttgttt cattttaggt aaatgttcga tttcatcctt tttctcaaac
     1201 aatagccgtt gagagatctg ttttgtattg tgatagataa actcgctata agctcgcata
     1261 taggcgaaca ccccatgagc aatcattttt ttctgaatga agtctcgaat caaaaattgc
     1321 ttttctttga aaacagagat agacaataca ttttttatca ataattgatt atcttccaag
     1381 cgaaaactac attcgttctc gtcatttcct acaactaacc aactttcgtc tcttaccgat
     1441 gctagcatta ttttttcttg aagctttcga agatcatacg gatccgcttg ttgtgtgaaa
     1501 gagcggataa atgttggctg gatcaacaga cgtgtcaaaa tagcccaatc gcttatttcc
     1561 tgtttttttt cttcttcatt taagtctgtg agctggatag tcatcaataa tttatcttcg
     1621 ccaatcattt tctcacctct ttaaaatctt tttataattt aattataacc aacgataaag
     1681 aaaaactaaa actttatgtc ttagaaacca atttggacga ttacatgata gactaagtaa
     1741 aaagaaacaa agaggtgttt acaatggggt tgaaatttga acgtacagat aatttagata
     1801 aacttttcga atcatttgct gtcgatccgg aaaaaacagt tgcaccaaaa gaagagatcg
     1861 atcgatttct aaaaccacag ccacaaaatg aaaagaaaaa agacaagaaa caaaagtaag
     1921 ttaagctcaa aaaaagactt accagccaaa cttttatgaa agagtttgac tggtaagtct
     1981 tttttattct ttttatgatt caattgcttg ttcgattgct gcccacgctt cttcttttcc
     2041 ctgttttgta acggaagaaa agagaataaa cgtgtcattg cgatcaaaat ccaacgcttt
     2101 tttgatttcg ctctcatgct tgttccattt accacgaggg atcttatctg ccttagtggc
     2161 aacaacaatt acagggatat cataatactt caaaaattga tacatctgga tatcttcttg
     2221 agaaggctta tgacgcaaat ctactaaaga aacgaccgca cgtagctggt ctctagtcgt
     2281 taaatatgtc tcgatcatct ttccccattt tgctcgctca gttttggata cttttgcata
     2341 cccatatccc ggaacatcga caaaatgtaa tgcgttctcg atcaaataaa aattcaatgt
     2401 ctgggtcttt cctggcttac cggaagtacg ggctaaattc tttcgatcaa tcaatgtgtt
     2461 gataaaagaa gatttcccta catttgatcg tcctgccaga gctatttctg gcaattgtgt
     2521 atctggatat tgttttggtg ccactgcact tatgataatt tcagcttgat ggactttcat
     2581 ggtctctctc cttaacaagg tgatgaataa agcaagcggc tgtcgcaaaa gccaaaatga
     2641 cttttacggc agcctctttt catcaacccg cttttttctc tgaatcttta tagattactg
     2701 ttggctcacc agacaattca gctgcttctt ttgtgataat cactttttcg atgttttcat
     2761 ttgaagggat atcaaacatc acatccatca tgatttcttc aatgatggaa cgaagtcctc
     2821 gagcaccggt attgcgttcg attgcttttt tcgcaatggc tctcaacgct tcaggttcga
     2881 attcaagttc ggtattatct agagaaagaa gtttttgata ttgtttgact agcgcatttt
     2941 tcggctcagt caagatccgt accagatcat ctgttgtcaa tttctctaaa gctgccataa
     3001 caggtaatcg tccgataaac tcagggatca atccgaattt tagtaaatct tcaggaatga
     3061 tatgctgcat gacactttcg tcttcagaaa gtttttggtt ttgtgtacca aatccaatcg
     3121 tcttttcgcc catacgattc ttcacaatcg tttcgatgcc atcaaatgca ccacctacga
     3181 tgaacaatat gttcgtcgta tcaatctgaa taaattcttg atgtggatgt ttacgtcctc
     3241 cctgaggtgg cacgcttgca acagttcctt ctagaatttt cagcaaggct tgttggacac
     3301 cttcgccgga aacgtcacga gtaatagata cattttcgct tttacgcgca attttatcga
     3361 tctcatcaat atagatgatc cctttttctg cacgttccac attgtaatca gcagattgaa
     3421 gcaatttaag caaaatgttt tctacatctt cccccacata accagcttct gtcaaacttg
     3481 tcgcatctgc gattgcaaaa ggtacattca atgtttttgc taatgtttgt gctaaaaatg
     3541 ttttaccaga ccctgtaggt ccgatcaagc agatattgct tttttgcagt tcaacatcat
     3601 ctgtgttttc ttgtgcttgc tgattgacac gtttatagtg attgtatacg gcaacagcta
     3661 acgagcgttt tgcgcgatct tgtccaatca cgtagttatt caatacttca agaatttcta
     3721 gtggtttagg gacttcagtg aattcgcgta cagcttcttc ataaaattct tcatcaatga
     3781 tttctttaca taggtcgata cattcattac agatgtagac ccctggacca gctacgatct
     3841 ttttcacttc ttcttgggtt ttcccgcaaa aagaacagcg tacggtctca ttgctacttg
     3901 gattgtcata catggtcttc accccttaac ttgcaaaaag tatgcaagaa actatcaaag
     3961 actttacagt cttttactat cataacatag tttagtaaaa agaaaaagtg tttaacaaag
     4021 gatcaactga cctatacctt tgcatttttt actcttaaca aaaaataagg aactgtgaca
     4081 aaatcgtgtc acagtccctt atttcgaata aacaattttc tagaagtagc ttcttcagaa
     4141 atgatttatt acttgatttc tattctttcg cttctgtcgc tgtacctgtg atcaattcga
     4201 ttgctttttt cattgtcaca tcatgttcca gcatatcttc tgtcaacacg cgtttgattt
     4261 gttcgattgg catgttgtat gtttctgata attcttggat ttctgcattg atttcttctt
     4321 ctgttgcttg aagattttca gcagctgcaa ttgcttcgat cacaagattt gttcttgtgc
     4381 gcatttctgc ttcaccttcg aattgtttat gcaagtcttc ttctgtagaa ccagttaatt
     4441 gatagtacat ttcaggagag ataccttgac gttgcatgtt gttcaagaat tcgtccattg
     4501 aacgatgtac ttcatcatga accatcacgt gtggcaattc aacgatttct gcgttttcaa
     4561 cagccatacg gatcgctgct tcatcttttg catcgtccgc tgctttttct tttgtttcta
     4621 ctaattcttt gcgatatttt tctttcaatt cgtctaatgt tgatacttcg tcatcaacat
     4681 cttttgcgaa ttcgtcatct aattcaggca cttcttttgc ttttacttca tggattgtca
     4741 ctttgaagac tgcttctttt ccagcaagat cttctgcttg ataatcttct gggaaagtta
     4801 catttacatc tagactttca cctgatttat gaccaactaa ttgatcttca aaacctggaa
     4861 tgaatgagcc tgaacctaat tcaagtgagt aattttcgcc ttttccgcca tcaaacgctt
     4921 cgccatctac aaagccttcg aagtcgatta ctactgtatc gccattttca gctgcagcat
     4981 cttctttgat aactaattca gcttgtgctt cttgttcacg tttgatacgt gcatccacgt
     5041 cagcatctgt tacttcacgg tcttgttttt caacaactag atctttatat tgtcctaatt
     5101 ttacttctgg tttcactgtt acttcagcag tgattaccca gtcttgacct ttttccatgc
     5161 tttcaacatc gatttttggt tgagaaacag gatcgatgcc agcttctttg acagcagctt
     5221 cataagcttc tggtaataca gcattcaatg catcttcata taaagcttct tcaccgtaca
     5281 tacggttgaa tacttgacga gaaactttcc ctttacggaa tccaggtacg ttcaagcttt
     5341 ttttcacttt attaaacgca gttgttaatc ctttttggat ttcgtcttgc gcaatcgtaa
     5401 attttaaaac accatcattc gtgccttttt tttcaaatgt tgcagtcatt taattccctc
     5461 cgagtttaga aattcatttt atacatcaaa aacgtttcat gcataccttt aaatagtaca
     5521 ctaacccttt caaattgtaa acatattgaa tggatagaat ccttgttttt cataacattt
     5581 ttatcttttt cactgataaa atctaatgga agaagatcat ttgctgcaaa attttttctc
     5641 ttttttttaa aaaatcttgt ttttcatctt ctgtaccttc atacatcgtt tttcctgtac
     5701 acgcctctac agtcagttga aaccagcgct tcgggtcttc gatgtatgta tcatagacag
     5761 gataaacaat tgcagattgc aacgtgaatt cttcatttaa cacggaacta agcagcgcat
     5821 cttcattacc gtaacgatct gaaaatacac ataattgctg gtaacttgcc tgctgctgcg
     5881 gctttggcag atcttttggt atgactttga ctaacttttc ttcaatcgtc aaatagaaaa
     5941 caggatggca ttcgttcaat tgcctaagtg tctccagtaa ttttgccctt gctaaaatat
     6001 ggacctctgg cgatacaata agtttactcg ctgtttggat aaactcatga taaggaagct
     6061 gttcgatctc atcaatagac gcaagctgac gtgcaggttc caacgaagga agttcctccg
     6121 atttattgtg gagtgccttg atcttctgcc tctccatttg tccggataat tcttctgcaa
     6181 attggatttt ttcaatgacc cgctgctcga aagcttcata aaaatctgta cttgctatca
     6241 attttctagc atataaaaaa tcttgtgtac tgattaacaa gtcaaaataa aattcagcaa
     6301 actcctcatt ttgaagatac tgtttttcat gcaagagtgc ttgcttcaat gcttcttttt
     6361 tctcgtccaa ctcgtaaaga caaaaaacaa gcaggctatt agcctcaaag ctttctttca
     6421 actcgtaagc tcgttgaaat aattcttttg cctgctgata ttgctggttt gctagtgctt
     6481 gttgtgctac tcgcattaaa ttctcatatt tttctggaaa atcaatcggc ttcacgcagt
     6541 gtcctcctct tgctcatgtt cagctcatgt tcaatagtag caaaacaaaa aaagcatgac
     6601 aattaaaaat aactgaagca ccctctaaaa gctagatttt agatctgact tttagaaggt
     6661 gctttcaact gttactgctt tttattgctc atcagctgtt gacttcctgg ttgaatgctt
     6721 tgatcagatt gatcgtaaca acacttgtat tagtgaaaaa atcgcctaga tgttgctttt
     6781 tctgattaaa gattgctgga agatacccta acatcagcaa aggaaatttt agaatgaacc
     6841 ggcaagcacc ttctctaact aaaacggttg accagctaag ttcttcttca tcaaagctga
     6901 tgactcgaat accaaagatc attttcccaa tcgtttgacc atgattgaat ttcgtcaata
     6961 aaataaaata cgctagataa ataagtaagg ctgtgaatcc gtagatactc agataagaat
     7021 ctgaaaaatt aagccaacct agatttttgg caataccaag tgttaaacgc gtaatagctc
     7081 cgatacacaa aagatcgacc aagaaagcaa acattcggat aaaaaaacct gcatagaaat
     7141 acttaggaaa atcatttgct tttggcggtt catttcttgt gtccgtatac tttttccatt
     7201 tacgttgttt ctttttgatt tcttcgggcg tcaaaggctc tctttcaaaa gagctcagta
     7261 ccttttggga aaagttggag tcaggtccta cagcaggctg ctgttgctta tctttttctt
     7321 gttccttatt ttcatttgaa agctctgaca tgcttactca cctccgtaat aatacattgc
     7381 ttttggtgat tccgctgtcc ctagattctc aagaacggat aagatctgac tagtctcaga
     7441 aggcttcaat ccctggaatt ctgctagttt acttcctaac catgtactag caaacccaga
     7501 tggcgtggaa gaatattcta ctagttccgc atcttcaagc tgttgttcct ttcgcatagc
     7561 ttttaaagca tcttcaggaa atcccaattc atcgactaaa cctacttctt ttgcttggac
     7621 accatcataa atccttccat cagcaatttt tttgacttct tcttcagatt tattccgtcc
     7681 ctcgctgacg attgctacaa aacgactata tgcgctgtcg atatatgctt gaagcaccgc
     7741 ttcttcttct tttgtctctg gtcgcgttcc tgaacccata tctttcaacg ctccgctttt
     7801 aactgttgta tcttccacac ccagtttttc tagaagtcct gaataattca atcctgacat
     7861 gatcacacca attgagccgg tcactgtttc ttctgttgca aagattttat ctgcttgagc
     7921 agaaatatag tagccaccgc tagctgccat gtttttcatg ctgacataca ttgggatatc
     7981 atgttcttta cgaatcgtat ccaatttctt agcgatttcc gcactttcgt agacaccgcc
     8041 accaggagaa tttacttcta aaaagatacc tttgactgtt ggatcttctt caatcgcctt
     8101 cagttgttcc ataaaatttt tatgattgta accttcagaa gcaaataaac ctgaagaccc
     8161 tcccgcagca atcgttccat ctactgataa ttttacgatt ttttgcgtac tgtctccctc
     8221 ttccaacaca tttgaagcta gttcttcatc cccatataac caactgttca atccctcaat
     8281 ttgatcgttt tcctgaggct tagtcaaact agctgaaact aacgaaacca caaataatcc
     8341 tgcagcaatc agaactgctg cccaacgtct cttattcatt ctttctcctt tctaatacaa
     8401 gttttttatg acgatcacag tttctgactg ctcagaagtg tcgaaccgct ctgctttttc
     8461 ttcccgcgca tacccggaaa acgactcttt tgtcaattca tcttctaccg taaactcacg
     8521 agcttcagca acttccaagg catcaggtgc gatttcttgg atgctatccc agccagtatc
     8581 taaaaatatt ttcgtatcat ctgatacacc agctgatgct tcgaatttgg acaattgttt
     8641 ttttaacatt cccagtgtca tgatctcaat ttttgaatcc atttttactc ctcctctatc
     8701 cgattacttt ctattgtacc gaactcctga aaaagatgat agataaaaac ccattatttt
     8761 ttgacataaa aagagcattt gacaaagcat ttgatatata ccaatttatt ttttattctt
     8821 tttttgttga caagaacatt cgcaaaaggt tatgatttta aatgtttcca gataaaaaac
     8881 aaaagaggag gatagttata ccttttagga tagcgccagg tctgtgtaaa cagatgacga
     8941 ggagagggtt tatcgaaaga ttcggcggat ggccctaggg actgcactct acagaaaatc
     9001 cacaaaaaat atctgcaaag ataaaacaaa aggattttcc tgcagctgga agcattcata
     9061 cgaattcatt atagaaagta ggatgttttt tatgtgcgga attgttggaa tgattggatt
     9121 aaaaaatgta acaccaggat tgattgacgg tctagaaaaa ttagaatacc gtggttatga
     9181 ttcagctggt atctttgtct cagatggcac aactgattat ctggtcaaag cacaaggcag
     9241 aattcaaaat ttgaaaaaca aaataaatat tgatactaca ggaaatatag gtattggtca
     9301 tacacgctgg gcaactcacg ggcaaccttc tgaagaaaac gcccatccac atacttccca
     9361 aagtggacgc ttcgtgctcg ttcacaatgg cgtaatcgaa aactttgaag aacttaaaat
     9421 tgcttattta gcaaatgacc actttattgg tgagaccgat actgagatta tcgctcatct
     9481 aattgaaact ttcgcgaagg atactacaac gcaagaggcg tttttaaaag ctttgcgggt
     9541 catcaaaggt tcttacgctt tcgctttgat tgatcggaca gcacctgatg ttatttacgt
     9601 cgctaaaaat aaaagtccac tattaatcgg actcggcgat ggattcaatg tgattgccag
     9661 cgatgcaatg gctatgcttg ctcatacgaa ggaattcgtt gagattgaag atgaagagat
     9721 ggttaccgtc acatcagaaa aagttattat tcaaaatttt gcaggtgaca tcgtagaacg
     9781 ttcttcattc gaagcacaag tagattcttc agatatcgag aaaggtacct atccttttta
     9841 tatgctgaaa gaaatcgacg aacagcctat tatcatgcgt cgtatcgctc aaaaatacgt
     9901 aacagaagac aatcgtgtct ctcttgatca aaagctggtc gatacattgt ctgatagcga
     9961 tcgaatctat atcgttgctt gcggaactag ttaccatgcg gggttagcag gtaaacagac
    10021 tctggaaaaa ttgacacaga tccctgttga ggttcattta gccagtgaat ttggctataa
    10081 cacacctctt ctttcaaaaa aaccattctt tattttcttg agtcaaagtg gcgaaacagc
    10141 agatagccgc caagttctgg taaaaatcaa ccgtctcggc tacccttcac ttacgattac
    10201 aaatgttgct ggttctactc tttcgcggga agcatccttt acactgctat tgcatgctgg
    10261 acccgaaatc gcagtagcat caacgaaagc ttatactgca caaatagctg tactaacttt
    10321 attagccaaa gctatcggtg ataaaaaaga atttgaaact tctcttgtat tcgatatcac
    10381 tcatgaattg agtttagttg ctaatgcgat ggaatcggtc atcgcccaaa aagattattt
    10441 agaagagttg gcagaagtat atctcagcga tacaagaaat gccttttaca ttggacgtgg
    10501 cgctgactac aacgtttctc ttgaagctgc tttgaaattg aaagaaatca gttatatcca
    10561 ggcagaaggt tttgccgctg gcgaattgaa acatggaacg attgctttga tcgaagaagg
    10621 aacaccagtg attggaatca tcagtgaaga agtgactggt gcgcatactc gaggaaacct
    10681 gaaagaagtc gaaagccgtg gcgctaaaac actagttatt gtctcagagg gattagaaaa
    10741 ggaaagtgac cagctggtgc taccaactgt ccacccttat ctaactactc taaccacagt
    10801 cgtaccgact caattacttg catattacgc aacgctatta cggggattcg acgttgacaa
    10861 accacgtaat ttagcgaaat cagtgacagt agaataataa aagaacaagg accagtgtca
    10921 gacatctgac actggtcctt gttcttttat tttttatgaa aatatctctt aatccagtcc
    10981 gatttcttct ttgactactg ccgcgatttt atcgacatag taattaactt tctcatcagt
    11041 aggcgcttct gccatcactc gaagtaatgg ttctgtaccg gaaggacgaa ctaggattct
    11101 tccttctgat cccatttcag cttctgcctc ttcaatcact tttttgattg ccggaacatc
    11161 catcgcaccg tttttatcac ttactcggat attcactaat ttttgcggat agattgttac
    11221 ctctgctgca agctctgata atttcttacc tgtctgtttc atgatattca gtaactgaat
    11281 tcctgaaagc atcccatctc cagttgtgtt aaaatctaag aaaatcatgt gcccagactg
    11341 ctcgccgcca aagttataat cgtttttgcg catttcttct acaacgtaac gatcaccaac
    11401 ttgtgtaacg acatctttca aaccgattcc ttccaccgct ttgtggaatc ctaagttact
    11461 catgacagtc gtaacgatcg tatctttttt caatcgattt ttttctgcca agtatttagc
    11521 acagataaac atgattttat caccgtcgac aatgtttcca agttcatcca ccgcaataat
    11581 acgatcgcca tcgccatcga aagctagtcc agcatctgct cctttttcaa cgaccatctc
    11641 agctaaacgt tctggatggg tagagcctac gccatcattg atatttaacc cgtctggatt
    11701 tgtgcccatg gtataaaaat ctgtttcaag atcggcaaac aaacgattaa cacttgttga
    11761 agttgctccg tttgctgcat cgacacaaat ggtcaaaccg gaaaggtcac cgttgatcgt
    11821 ttgtacaagg aattgagaat atttcaataa gccttctgga aattcttcta cagttcccaa
    11881 tccttcagct gatggacgag gcaattcatc cacttcagca tctaataatg cttcaatttc
    11941 tagctcttgg tcatctacta gtttaaaacc atctgcaccg aaaaatttga ttccattatc
    12001 ttgtgctgga ttatgagaag cagaaatcat gacccctgcg cttgcttttt gcaaacgtgt
    12061 cagataagca acacctggtg tagagatcac acctagctgg aacacttcga tacccaccga
    12121 taaaagtcct gaaatcagcg cttcttctaa catttgacct gagattcttg tatcccgccc
    12181 tactaacacg cgtgggcgtt ttccttcttc ttcatgctgg cttaggacat aacctccgca
    12241 ccgtcccagc ttaaatgcaa gttctggcgt aagttccttg ttggcttcac cgcgtacgcc
    12301 gtctgttcca aaatatttac ccatagttgt aaaattactc ctttaactta attttctgag
    12361 gaatttgttc tgctcatttg tgttgattcg cttgtttctg atgtttcttc tgtacttgtg
    12421 gtcgagctgc ttgtcccaga ttccgaactg gaagaaatgg ttgttgtcga tggttccgtt
    12481 cctgaatcag cagatgaaat acttctactc gtacttgtat tttcttgtgt ccgactgctc
    12541 gtcgaagcag agctactgct tgacgtttgc aacacgggtg taatctccac actaactgat
    12601 tcgggcttga tacttaaccc ctcttctact ggtatttcaa ttttacgagt tgttcgatga
    12661 ttgatacctg agacattgat cggtacagca atactgtcac cgatcgatgc aagcaaatcg
    12721 ctagagccgc ttacttctgc ttccagtttt gaaaaactga aatcatagct ttctaccccg
    12781 gcagctggtg ttccttgctg tatcccataa agtcgtaccg acttgcttgg cttgatcgaa
    12841 tcagctctca ctgttacttg cactggatca gagacgatac ttaatacttc ccctgctgca
    12901 ttcagcgcct gtattgggac ctcactatta atcccatcat ctgtgaccat cgaagcgtcc
    12961 acatcagcta ctactcgttt gatttctgcc aatgttttgt cacctgtagt gatagagact
    13021 tttttaggac taacggaagt atcatcaagt tcatatccag atggtgtgaa cgttgaagga
    13081 atcaacgttt ccacatcgaa atcttttatt acctttttct caaccgttac tgtaatagtc
    13141 gatggttcga tctttgctgt gacagcactg ctcaaatttt gaatttttag cggaacctca
    13201 tgtgttcctt cgcccagctt agtgatatct gctgtgacgc ggaacatccg tgtatcttcg
    13261 tttgcctctg cattcaactg aactcggttt gcactggaaa gtttgacagt cactgcattt
    13321 tcatatccat ggatatagta gcgctttgaa tcataagaaa tcttgattgg caccccagta
    13381 actgtttgat tatacgcttc atttccagaa agcgtatttg gaacggactg gccatttgca
    13441 ttgaagtaca gcgcaatact gaacagcaat gcaataaaac tgtaaagaat gcttgtccgt
    13501 ttctcttttg agatcatttg cgatcccctc ccttagtcat accgtctagg aaagattgaa
    13561 tcacgttttt cttgtttttt gttttctctt cagcaatcag ttctctacgc aaaattgcca
    13621 aatattcttg ctgggataag ccagccatca gttcattatc taatgtaatg ctgacatccc
    13681 cagtttcttc agagacaacg atggtaatcg cgtcgctgac ttcgcttata ccgacagctg
    13741 cccggtgtcg agtcccgaat tcttttggaa tcaacatact ttctgataga ggaagatagg
    13801 cgcttgctac tgcgatcttt ccttctttta caataactgc accgtcatgc agcggtgtat
    13861 taggaataaa gatattgatc agcagctctc cagtgatatc tgcatctaat gcaattcctg
    13921 tctcaatata ctcatctaat ccagtatgcc gttcgatagt aattagcgca ccgatttttc
    13981 gcttggacat atattggatg gctttgtcaa aagacaagat catcttctca tcttcctgct
    14041 gttcgctttt tgattgtttg aaaagcgaac tgcgtccaag atgttccagt cctctccgga
    14101 tttccggctg aaaaatcacg atagcagcta tcaccccgta cgtaatgact tggttcatca
    14161 gccaagacaa ggtgtgcaga ccaatgattt ccgctacgat tcggataaca ataaaaatag
    14221 ctacaccttt cagaagctga atcgcttttg tgcctcgcag cagttggatc agtttgtata
    14281 caagatacca gacaaccaga atatcaagta tgttcaccaa aaaattcaga gagaacaagt
    14341 caccggaaaa catttgctgc caatagtcca aattaaaaag ttccccgatt tgaaatgaca
    14401 taaaaagcct ctcttctctt ttgagatttc ctctctagta tatcatatgt tttaatttta
    14461 gaggctgatc tgctcgtatt tttcttgaga aaaacgcata attttcagga aaattgcaag
    14521 tatgcgtttt atttttctct gcaataaata tttttaaact tcagagaatt attcattata
    14581 ttttttctgt tatttttcct ttttttcttt tgtagcttgt tccatttttt tacgtcgttc
    14641 tgctctggca gctcttcttt cttctgcttc aaggtccgtc gttttttctt ttctagtctt
    14701 aacgggtaca tcgatagatt cctctggttc aagtcgtttc ttgatttttt ctaattctcc
    14761 cgtcagtgca gcatagttgt aaaaacgtgt tttagcgtct tggatagtct tttcaaataa
    14821 agcttttgca agatttggat ttgcgttctc tagcgaggaa aagcgaactt gtttcctcat
    14881 gaaatcaggc atagcagaga aatcaggttt tttgtagtcc aaagtcattg ggttcttgtt
    14941 tttttcgacc attgcagggt tatatcgata taatgaccaa tagccagaag aaacagcttc
    15001 ttttgcttct ttaagcgatt ctttcattcc accaagcaaa ccatgtgtga tacaaggcgt
    15061 ataggcaatg attaaagaag gtccaggata cttttctgct tcttcaaaag cctttatcgt
    15121 ctgcatttga ttggcacctg aagcaatttg cgccacataa acatttccat atgtcatcgc
    15181 catcattcct agatctttct ttgaagcata ctttccacta gccgaaaatt tagcgattgc
    15241 cgaagctggt gttgcttttg aggtttgacc gccagtattg gaatagactt cgttatccaa
    15301 aacaagaata ttcacgtctg ctccgctggc aagtacatga tcgatcccgc caaaaccaat
    15361 gtcgtaagcc catccgtctc caccaatcat ccattggctg ttttttacga atagatcctt
    15421 tctcttgtat atatcctcca gttcagggac aattgatcgt tcgctttcga tcgcagcttg
    15481 cagttttgct gctctttgct gcgttccttc gctctcaaac atgtgtacca accagtcttc
    15541 catcaattct cgtagttctt ctgatacttt tggcaatact tctttgattg aacttgccaa
    15601 agcctcgcga cgagtctgtg ccgctagaag cataccataa ccaaattcag cattatcttc
    15661 caataaagaa tttgaccatg caggcccttg accacattca ttggtcgtat aaggagaagc
    15721 aggagaagca ccgccccaaa tagaagaaca gcctgtcgca ttcgcgatca tcattcgatc
    15781 accgaacatt tgtgtcaata attttacata aggtgtttcg ccacagcctg agcaagcgcc
    15841 cgagaattca agaagcggtt gattgaattg tgtagacaag acagtattct ttcctttgac
    15901 tggatttgct ttttgtctta atgtcattgc aaaagcccaa ttcacagctt gttctttctg
    15961 ttcttcataa ggcttcatca ctaaagcttt ttcttttgcg ggacaagcct ctacacacag
    16021 accacaacct gtacagtctt ccactgaaac ttggatacgg tattttacgc cgtctgcgcc
    16081 tcgcatttct ctcacgataa atccttctgg cgcttcttcc atttcctctt catctgctaa
    16141 aaatggacgg atggcagcat gaggacaaac aaaggcacat tcattacaca tcgtacagcg
    16201 atcactaatc cactcaggta cttctaaagc gacgccccgc ttttccatag aagccgttcc
    16261 taaaggcatt tcgccagttg tcattccatt tgctacaagt gtaccaacag agagttgatt
    16321 tccttcctgt gcattcactg gttcaagtat ttcttgtata taaccagtgt tccttttatt
    16381 gattttttca ggcatctcca acgttctcca atggtctggg atcgcgactt gatgaagcag
    16441 ttcggtcgtc cgatcgattg cttgtatgtt tttttctacg atagccattg atttatgtcc
    16501 atagcttttc agggcttctt cttttagcag cggaagcacc tcatcaaaag gaatgatgtc
    16561 agctaattta aaaaaagccg tttccattgc cgtattgatc cggcgtccca aaccgacttc
    16621 ttgggccaat ctcatggcat tgatcgtata gaatctaatg ttattttcag ccaagtaacg
    16681 ttttaatttt aagggcagat gcgtttctaa ttgttcgtcg ctccaaagag tattcaataa
    16741 aaaaatgcca cctggcttca atcctttgac caagtcatag ctatgcaggt aagctggtgt
    16801 gtgacaggca acaaggtccg catgctcgac aagatacgct gaacgtatcg gcgtatctcc
    16861 aaaacgaaga tgagagacag tcagaccgcc agattttttc gaatcataat aaaaatagcc
    16921 ttgcgcatat ttatcggtgt gatctccaat aatcttgata gctgatttat ttgctccaac
    16981 tgttccgtca gatccaaatc cccaaaactt ggcctggaat gtttgcggtt ctgtcaaatc
    17041 cagactttcc tttggttcca aagattgata agtcacatca tccacgatcc ctatggtaaa
    17101 ccgttttttg cgtatgcttg ggtctttttt caattcatca aaaactgctg agatttggtc
    17161 gggagtcaca tctttagacc ccaatccgta gcgaccacca ataacagccg ggcgaagttc
    17221 agaatcatat aacgcacttt gaacgtctaa taagagaggt tctccacctg cccctggttc
    17281 tttacttcga tcaagaacag caattgtttt tactgtatga ggcaactttt gcacgaatgt
    17341 ttccactgga aaaggacgat aaagatggat attcaaaaaa cctgttttcc gtccgtttgc
    17401 attcaagtaa tcaatcgttt gttcaatcgt ctgtgcaact gatcccatag aaacaatgac
    17461 ttcttccgca tcgtctgctc cgtaataagt caccaagtca taagaagttc ctcgtaatcg
    17521 gttgatctct ttcatatatt tttgaacgat ttcaggtaat ttatcataat aggggttgat
    17581 cgtttcccgt tgttgaaaat ggatgtctgg attctggttc gtaccactaa cagtcggatg
    17641 attgggattc atccctcgtt tacgaaatga agctaatttc tcctgattga tcagaggagc
    17701 taattcttca tattccaaaa cttcgatttt ttgaatctcg tgactcgtac ggaatccatc
    17761 aaagaaattc aaaaaaggta cttctccttc aatagaagca agatgggcaa caggtgctaa
    17821 atccatcact tcttgtacac tgctttcagc taacatagca aatcctgttt gtcttgcagc
    17881 catcacgtct ccgtgatctc cgaaaatatt caacgcattt gtcgtcactg cccgactagc
    17941 aacatggaaa acacttggca gtagttcccc agcaatcttg tacatattcg gtatcatcaa
    18001 aagcagccct tgagaagcgg tatatgtagt tgtcagtgca ccagttttca atgatccatg
    18061 gaccacacca gctgctcctg cttcggattg catttctact acttttactg gttgaccgaa
    18121 tacattttct tttcctgctg ctgaccattg atctactaat tcagccatgg tcgaactggg
    18181 tgttattgga tatattgcag caatctcggt aaatgcataa gaaatatatg cggctgccgt
    18241 attgccatcc attgttttca tttttcgcat gattcatcat ccttgttcat attctctctt
    18301 attatctgtt ccattataac gtaaaacatt ccattttttc ttttatttat aaaaatattt
    18361 cacatatgtc tatttgaatt ggtcatctaa tttttagaca aacagtctaa atcgttgcct
    18421 ttttttgtca gacatgtata aactataagc gaaagaggtg ttttaacatg gatatatcag
    18481 tgattgatgc aacgaaaaca aatacacaaa aaggaatcct ttatggttca tcgaatgctc
    18541 ctaaaaaaat ggtcgaattc atcaacctat cttgtccgta ttgccgacaa tggtttgaag
    18601 aatcttatga gctattagaa gaagcagtcc aaagcgggca attgcagcga gtgatcaaat
    18661 tgttcgataa agaaaaagaa agcttgcagc gaggaaacgt gatgcaccgg tatctcacaa
    18721 tcagcgatgg tcaaaaagcg atcaaagaga tcaaacaaat attcgatact caagacgagt
    18781 ggaaacatct ttctctccaa gaagtagcgg attttgcagt agataaacta aagctgacag
    18841 aacaaaaaga cgaacagctg agccaagctg tcatcaacga agctgagcaa gcacatattc
    18901 gatttgttcc gactgtcatc ttgggtaaag aaatctttga tgaatctatt tcaatagagg
    18961 aattaaaaga attgatccaa acaaaataaa aaaataagga gcgtaggaca aaagtaaaga
    19021 acacttttgt ctcacgctct aaacacctat aaacggcgga agcagaagca actccttctt
    19081 atttcttgga gttaaacgct tctgtcccag cctccttatt ccgaataaat ggtgtccaaa
    19141 agcttgcatc tgccagatta tctcatttct tatgacaagc tgagcaaatt atctccacca
    19201 aataatttat tcatattttt gacttgcgca tactaactaa ttcttaaatt ttcatacaaa
    19261 atttctcaaa ttcccttaac gagtaatatt tatgtacggt gattcacact agtatcccaa
    19321 tcacttttta agttatcccg atttcaattc tacttcaaaa atagttgctt ttatgtatgc
    19381 tgttagttga ttagatggtc gtttgttgag ctaggtcgtt tattttaaac aagttgtttc
    19441 tttagcttat acctatctcg tctaccaaac gcaagatggc aaacacaaat gcatacgcat
    19501 ctgtatttgc cactttgatt cgtataaagc taacaactcc taactcctct catttaatag
    19561 ttgctctctt ttttattcag atacattttt gctgatcaca gcctagtatt tcaatggttt
    19621 tgaacactaa accaaattgt tccattacaa cattcactgg ctgatgaaat ggattaatag
    19681 atttcgatta ttcaaagcga ttttctattg gtggattgtg ttcccaacta acaagacgga
    19741 ttccaaagtc attatataac tcttcaagag ttaacccttt cctgttccca atagatgtat
    19801 ctctcccacg ctcgatataa aatatggagt ttcgaatgcc aagacctggc aaatctttcc
    19861 ttgttacagt tacactgcta ctaacttccc catataaaat ccagctcctc caaaatgata
    19921 atatattttt cgaccatcat ctaactcatg taacaataat tgttgtgccc tcttgtcact
    19981 cgccaaatgc aaacgccacg tgcgatacca tgccatgcta cctgcatctg cagaaaatcc
    20041 atcaaataat gcaacaattg tatcttctat cacaatcctt tccccatcta cttcatattc
    20101 taatctaaaa tcaaactccc ctcgggtaat cgcaggtctt ggtggcttag gacccaatat
    20161 tattctactt aaaaccgcat tcgttcccag atgaaacaca gctataacaa caataatgat
    20221 taatattttg tttctatttg ataaatttct cattattcgc aaatcacctc tacattctca
    20281 catactagtt cgtaatactc ctttaatatc aaaatatcta ctgatcgtaa taatattcgc
    20341 tatgaattcc tactactcgt ataagaaagg ccaaatcatc agcttttact tgctgatgat
    20401 ttggcttgtt tctatttatc gtaattgttt tctaatcacc aaaattgtta acaatcggtt
    20461 cagatatttc ccaa
//
LOCUS       DAGCYL010000032        20095 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.033, whole genome shotgun sequence.
ACCESSION   DAGCYL010000032 DAGCYL010000000
VERSION     DAGCYL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.033"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            182..1948
                     /locus_tag="MB216_000909"
     CDS             182..1948
                     /locus_tag="MB216_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBT4525766.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1963..2400
                     /locus_tag="MB216_000910"
     CDS             1963..2400
                     /locus_tag="MB216_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4525767.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2413..3564
                     /locus_tag="MB216_000911"
     CDS             2413..3564
                     /locus_tag="MB216_000911"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBT4525768.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(3626..4324)
                     /locus_tag="MB216_000912"
     CDS             complement(3626..4324)
                     /locus_tag="MB216_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBT4525769.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(4317..5669)
                     /locus_tag="MB216_000913"
     CDS             complement(4317..5669)
                     /locus_tag="MB216_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBT4525770.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(5892..7292)
                     /locus_tag="MB216_000914"
     CDS             complement(5892..7292)
                     /locus_tag="MB216_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBT4525771.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            7453..8190
                     /locus_tag="MB216_000915"
     CDS             7453..8190
                     /locus_tag="MB216_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBT4525772.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            8315..8794
                     /locus_tag="MB216_000916"
     CDS             8315..8794
                     /locus_tag="MB216_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBT4525773.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            8791..9558
                     /locus_tag="MB216_000917"
     CDS             8791..9558
                     /locus_tag="MB216_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525774.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEELVVYSRSQRRRHS"
     gene            9788..10357
                     /gene="lepB"
                     /locus_tag="MB216_000918"
     CDS             9788..10357
                     /gene="lepB"
                     /locus_tag="MB216_000918"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBT4525775.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(10461..11063)
                     /locus_tag="MB216_000919"
     CDS             complement(10461..11063)
                     /locus_tag="MB216_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBT4525776.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            11169..11638
                     /locus_tag="MB216_000920"
                     /pseudo
     CDS             11169..11638
                     /locus_tag="MB216_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            11744..12328
                     /locus_tag="MB216_000921"
     CDS             11744..12328
                     /locus_tag="MB216_000921"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBT4525777.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            12331..13404
                     /gene="prfA"
                     /locus_tag="MB216_000922"
     CDS             12331..13404
                     /gene="prfA"
                     /locus_tag="MB216_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBT4525778.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            13397..14236
                     /gene="prmC"
                     /locus_tag="MB216_000923"
     CDS             13397..14236
                     /gene="prmC"
                     /locus_tag="MB216_000923"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBT4525779.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            14229..14408
                     /locus_tag="MB216_000924"
     CDS             14229..14408
                     /locus_tag="MB216_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525780.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            14430..15455
                     /locus_tag="MB216_000925"
     CDS             14430..15455
                     /locus_tag="MB216_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBT4525781.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            15630..16874
                     /locus_tag="MB216_000926"
     CDS             15630..16874
                     /locus_tag="MB216_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBT4525782.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            17027..17974
                     /locus_tag="MB216_000927"
     CDS             17027..17974
                     /locus_tag="MB216_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4525783.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            18107..18736
                     /gene="upp"
                     /locus_tag="MB216_000928"
     CDS             18107..18736
                     /gene="upp"
                     /locus_tag="MB216_000928"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBT4525784.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(18875..19117)
                     /locus_tag="MB216_000929"
     CDS             complement(18875..19117)
                     /locus_tag="MB216_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525785.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            19238..19310
                     /locus_tag="MB216_000930"
     tRNA            19238..19310
                     /locus_tag="MB216_000930"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19271..19273,aa:Thr,seq:cgt)
     gene            complement(19429..20067)
                     /locus_tag="MB216_000931"
     CDS             complement(19429..20067)
                     /locus_tag="MB216_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBT4525786.1"
                     /translation="MNNILGKKIELKKGILGTIVVVFGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         6835 a         3583 c         4087 g         5590 t
ORIGIN      
        1 atattataaa aaaattaaaa taacagtttt atttaaaatt gaagttttct gataccgaaa
       61 tctttttatt ttgctgacat actcaaacct ttggcacaaa gtttcgtgcc aaagtacgac
      121 tatttctctt tttagtaagc ggatacaatt agagtatcaa aaggagagag ggagagacaa
      181 aatggaacgg acgattccca aaaacaacat ttcgctaaaa gcgagggtac aaaaattagg
      241 cagtacttta tcaagcatgg tcatgccaaa tattggagca ctgattgcct ggggtgtatt
      301 aacggctcta tttatcccag atggttattt accaaatgag gctttcgcta ccatggtaag
      361 cccaatgcta acttatctga ttcctttatt gatcggttat actggcggaa aagtaattgc
      421 tggtgaccgc ggatcagttg ttggggcaat tgcaacgatg ggtgtgattg tcggaacaga
      481 tattccaatg atgctaggcg cgatgatcat gggacctctt ggtggatatg ccatcaagaa
      541 atttgatcag ctattccaaa aacggatcaa atccggtttt gaaatgctag tcaataattt
      601 ttcagcaggt cttatcggtt ttggattagc gttattaggt ttttctgcga ttggtccagt
      661 tgtcgatgca ttgactcaag caatggcaaa aggtgtagag atcattttat ctgcccattt
      721 gattccattg acaagtattt ttattgaacc agcaaaaatc ttatttttga ataatgcaat
      781 caatcatggg attttgacac cattgggaac tgaacaagta gcagaggctg gaaaatctat
      841 tctattcttg cttgaagcaa atcctggtcc aggacttggc gtattacttg cttttacttt
      901 gtttggcaaa ggagcagcaa aatcttcagc accaggagcc atgatcattc actttttagg
      961 cgggatccat gaaatctatt ttccttacgt tatgatgaaa ccgttattat tcttatcagt
     1021 tataggcgga ggaatgtcag gaagtttcat tttccaattg atggatgctg gactaagagc
     1081 gccagcttct ccagggtcga tcattgcgat tctagcgatg acaccgttca attcttacct
     1141 tcctgttatt ttgggcgtga ttgttgccgc agctgtttcg tttggtattt ctgcagtaat
     1201 tttgaaagcg gatgcgaaag atacaaatga agacttcgaa aaatctgttc aagagacaaa
     1261 ggcagctaag caagcttcca aaggtgccca aggtattcct caaggagcta cattctctgg
     1321 cgtacaaaaa atcatttttg cttgtgatgc aggaatggga tcaagtgcga tgggcgcttc
     1381 gatcttgaga aaaaaagtac aggaagcaga cttgcctcaa acggtgacca atcaagcaat
     1441 cagccaactg acagatgact cgaatacatt gatcgttact caggtcgaac tacaagaaag
     1501 agcaaaacaa aaagcaccga gcgcgcgttt tgtctcagta gaaaactttt tgaattcacc
     1561 tcgatatgat gaaatcattc aagaattaag cagcaagcag aaaacatcag tttctgaaat
     1621 tgagaatgag ccagcagaag agtttctttc agcaaacatc aaacaaatca aagagattct
     1681 acttgtccac gatgatcgtt ccggatcagc aacaatgggg atgaccgtgc tcaaagatat
     1741 attagacaag aaccatttaa ctatcccgtt aagaaaagtc aatatccaag atttaaaaga
     1801 tgaacctgat tcactgatcg tagccaaaga aagtttagcg gaaaaagtac agatggctgc
     1861 acctcaatcc attcatttat cagtcaacag catggtcaat ccgcaaaaat acgaatcgat
     1921 tgcacaatat ttgaaacaga cagcttaagg gaggaaaaca gcatgcaaag tttgccattc
     1981 gacatgatcg agctcaatcg atcttttaaa accaaagaag aagcgatccg ctattgcggt
     2041 cagatgcttg tagaagcagg gtgcgttgat gaggaatata tcgatgccat ggtagataga
     2101 gacaaaatgt tatctgttta tatggggaat tttatcgcga ttcctcatgg aacagatgaa
     2161 gcaaaaaaat atgtgaaaaa atccggcatc tgtgtcgttc aagtaccaga tggtgtagat
     2221 tttggtaccg aagaagaaga aaagattgcg actgttttgt tcggtattgc aggagtcgga
     2281 gatgagcatt tgcaacttgt acagcaaatc gcactttatt gcagtgatat ggacaatgtt
     2341 gtccagctag cagacgcgct cagcaaagaa gaagtaacgg aaaatctagc aattgcttaa
     2401 ggagagagaa aaatgagagc agtacatttt ggtgcaggaa atatcggtcg aggatttatt
     2461 ggagaggttt tggcagctaa tggatttgag attactttcg tagatgtgaa tcacacgatc
     2521 attgatgctt taaatgaaag aaaagaatac gaaatcgaat tagcagatga atcaaaagaa
     2581 aaaatcactg taaaaaatgt caaaggaatc aacaatcaaa caaatccaga aaaagtaatc
     2641 caagaaatcg ctgaagcaga tttagttact accgccattg gcccgaatat cttacctttt
     2701 attgctgagt tgattgcaaa aggtatccaa gaaagagaaa aggaaggaag cacaacatct
     2761 ttagatatca ttgcctgtga aaatatgatt ggcggttcag catttctaga aaaagaagtg
     2821 tataaatatc ttccggaaac atcatttact gacaaatata tcggctttcc aaatgcagca
     2881 gtcgatcgaa tcgtcccatt acaacaccat gaagatcctt tattcgtcca agtggaacct
     2941 ttcaaagaat gggtcattga cgatagccaa agaaaaaata aaaaaatcca attaaaaggg
     3001 gttttatatg tagatgatct agaaccatat atcgaacgca aactatttag tgtcaatact
     3061 ggtcatgcga ctgtcgctta tacaggtgcc ttgttaggtt accagaccat tgatgaagca
     3121 atgcaagatg cgttagttgt ggcacaatta aaatcagttt tgcaggaaac aggaagtttg
     3181 ctgattgcca aatgggggtt tgatgcagag cagcatcatg cgtatattga aaaaatcatt
     3241 catcgcttcc aaaacaaaaa tatctctgat gcgatcacac gtgtcgctcg gacaccatta
     3301 cgtaagttag gttatcaaga aagatttaca cgtccagtga gagaactaca agaacataac
     3361 ttgacttgcc cacatttgac ggcaacaatg gggattattt ttaattacta tgatcctgaa
     3421 gatgaacaaa gccgtcaatt gcatgaaatg aagatccatg aaaacttgga acaattgatt
     3481 caagaagtca caggaatcaa tgatccgaaa acaatcggga atatcaagca aaacgtgaac
     3541 cgttatgcca agcaagtcgc ttaaaatagg atacgaaaaa ggggaatgtg acgaaatttt
     3601 tgtcacattc ccctttttcg aataactagg ctgtttgtcc ttcaagatag ggcagatcca
     3661 attctttacc gtaacgttgt tctaaagcta ggcggatcaa acgagctgcc aggttctcat
     3721 ttcttgcaag gataggtcca tggaaataag aaccgaagac atttcggtaa atgacacctt
     3781 cagattggtc ttcaccatta tttccttgac cttgtaccac tttccccaaa ggtcgttcac
     3841 cttcacctaa gaaagtccga ccattgtggt tttcaaatcc ataataagtt tcattgaatt
     3901 cttcgttatg gataacgata tcaccgataa accggttatt ttcctgactt aacgtgtagt
     3961 gatccagtgc actgattcct tggatctttt ctccattagc tcccatgtag tagtgaccta
     4021 gcaattggta gccaccacaa atggctaaca tgacgccatc attttcgata tagttgatca
     4081 aagaatcttt ctttttctgg atgtcttttg aaatgattaa ttgctcaaag tcttgaccac
     4141 cgccaaagaa aaccaagtca tatttatcag catcaaaagg ttcatgtata ctaacgatct
     4201 ccgtatggca agaaaccccc attttttctg ctatatattt caacatcaaa aggtttccgt
     4261 tatcaccata tgtgttcatc aaatttccat aaaggtgggc gatattgagt tcataattag
     4321 ccacggttca ttcctccttt gataaatcct tgagcagcta gttctttacg aagctgcaat
     4381 acagcagtgt aagtagctaa aatatagaca tgctcagttg gcaattcttt gattttttcg
     4441 attacttctg gtaattcttt tgtttctgtc aatttatttt ctggaatccc ggcaactttt
     4501 agacgtaaag tcatatctgt atgacggtct cctccagaaa taacagctgg gatatccata
     4561 tcggcaaagt tttcaaaatt accatcccaa atccagctga catcgattcc gtctgcgtaa
     4621 ttggcattta ataaagccac taaagaaaat ggataaggag ctagaccaat catatcgatt
     4681 acttgattga tacctacagg atttttgaca agtactaatg tacatttttt cccattgata
     4741 ttgatgactt cttgtcggcc gaatactttc tcgtcatatc ctaaaccttg tttgatttta
     4801 tcaggatcga cattgaagta ctctgccact gcagtagctg ctaaagcatt gtagacatta
     4861 tacataccgc caacttcgat tccgtattca tgaccatcga tcacgaaatc tgctgacaca
     4921 ttgtccatcc gtaccatatc agttaatcga taatccaatt caggacgttt gaagtcacaa
     4981 tttggacagt aatatttccc taagtttgca taagtgatca ttttataatg caagatgtga
     5041 tgacaatttg gacataagac accgtctgta ttgtaatgag ccatttgttc acgatcttct
     5101 tcatggtcaa agccataata ttttctagga ttgactgttt cgatagaatt gaagattggt
     5161 gaatcaccgt tactgataat cgttgcattt ggtgactttg cggcaccgtc tacaatcatt
     5221 ttataggtcg tatagatttc tccataccga tccatttgat cacggaaaat atttgtaaat
     5281 aagaacagtt ctggtttcat atattctgtg actttgctca aacttgcttc atctatttct
     5341 aggacagcaa actttttctg tccttttttt ggttttgcct gcaagaaagt cgatacaatt
     5401 ccctgtacca tattcgcacc agtagggttg gttagaactt catcgaattg ctggcgcaaa
     5461 atattgactg ttaaagcggt agtcaatgtt ttaccgtttg ttccggtgac aacgacgact
     5521 tggtagtctt ttgccaaact atctagtata tgtggatcga ctttcagcgc tagtttacct
     5581 ggtaaacttg atccgccttt aaaaaaagtt tttaatgccc attgcgacgt ttttccaact
     5641 gcaatcgcaa aatgactgcg tattcccatt atgtaacctc caaaaacgat ttgctgacga
     5701 ataaattcaa ctaatcatac catatgttga aaaaaaggtt aaattgcttt tcttattctt
     5761 aagaaaaggt tacggctgaa agtgcatgaa atcggacaaa atcgcatgat ttaacaaaaa
     5821 aacagcttga aacaaaatga ttgtttcaag ctgctgtgat tttattattc cctcttacct
     5881 atattcccat tttaaccgat aataattttc tcgcttggat attcatagcc tagatctcgt
     5941 gtttcttttg gcacgaataa catcaatgta tatagcattc cgatccgccc aataaacatc
     6001 agtgtagcaa tcgtgatttt gcccacaaca gacaagtctc cggtaatccc taaagataaa
     6061 ccagttgttc caaacgcaga agttacttcg atgatgatcg agatcagcgt ttgttcttct
     6121 gtagcagata aaaaaacgat acaaaagaaa cacataccaa gagaaagcat aaagacaacg
     6181 actgattttc gcacatcatc ttgatcgatt ctccgtccaa aaatattgat gttatcttca
     6241 cttttgagga aggaatatag atacaaaccg atgattgcta cagtagttgt tcgaatcccg
     6301 ccgcctacag aactaggact gcatccgata aacatcagta aagaaaaaat gatcagtgtt
     6361 gtgatttgaa aatcacccaa atcgtgaatc tgcagaccag cattacgagt cgtaatcgaa
     6421 taaaacatcg aattgatcca tttgacactc atattggaat cttggaataa atggtccttt
     6481 tccaatagat aaatcaagac agttcccgaa acaaacaaga taacaaaagc taacacagct
     6541 aatttggtaa acaaggaaaa acgaaacgga agcttcgcat tggaacgttt gtataatagc
     6601 cattcccggc actccatcaa tacaggaaaa ccaattcctc cgacaaaaat caaaaacatg
     6661 atcaggatca agaaaaaata gtcatgtgca aagggcttga tggaatcccc agtgacgtca
     6721 aacccagagt tcgtgacagc cgatatcgcc tgataaaaac cgtaaaaaac ggcatcacgc
     6781 caacgatcaa aatagcctct gtaataaaaa taaatagaaa agaatgtccc aaaaagtaat
     6841 tgaaaccaga ttaaaatagc aaaagtgatc cgaatcagtc ggacgatacc agacaaacgc
     6901 ggttgattca tatccgtcat gatcagctgt cgctgtttca gtgtgatccg tcgtttagaa
     6961 aaaatgacaa aagcagtaga aatcatcatg atccccaaac caccaacttg aaataatatt
     7021 tccagcagta taataccgtt atcattgaac acagaattga tatcaaaagt cgatagtcca
     7081 gtcacactca ctgtactgat tgccatgaac agcatgtcca caaaagaaac atggctatct
     7141 ggttcgcgaa aaatcggcat ataaaataga acgagtgata agaccgtcat caatatataa
     7201 taaaaaacga tgatttggat cgatgagaaa tgattggcag caaacttctg tcctcgccgc
     7261 tgcagccttc gaaagaataa atcaacatgc acagtactat cctcctgaca atagtcgttt
     7321 ctaccagtat acaaggggaa ttaggaatac tcaaagaaat tatcaaacag ataacaatac
     7381 agctttcgat taaaatagaa atggcttata ataaggaaaa tcctagaaaa aagaaggagg
     7441 aggaataaca aattgattaa tgctcaacaa atcgtagaaa aaatgaaaaa tcaaaaaata
     7501 aattatgata cagttctgaa aaaaatgatc atgcaatggg agaaagaaga agtaaagcct
     7561 aagatattaa tacatacatg ctgtgctcct tgcagtacgc acacattgga attcatgtct
     7621 caatatgcag agatcacgtt atttttttca aattcgaata ttcatcctaa aagtgagtat
     7681 ttgcgaagat tacatgaaca aaaacgattt gtagaccaat ttaatgagaa aactgaccat
     7741 catgttgcgc taatcgttga tgaataccgt ccaaatgaat tcaccaaaat ggttttaaca
     7801 aaccatcttg aaaacgaaaa agaaggtggt gctcgctgca gtgcatgctt caatatgcga
     7861 ttagatcttg ttgcgcaaaa agcgcaagaa ctaggatacg attactttgg aagtgccctg
     7921 accatctcgc caaagaaaaa tgcagctttg atcaatcaga tcggaatgga tatccaaaag
     7981 atttacggga cacaatactt gccaagtgat ttcaagaaaa acaaaggcta tgaacgctct
     8041 ttgcaaatgt gcaaagagta tgatatttat cgccaatgtt attgtggttg cgtgttcgct
     8101 gccaagcagc aaggatgcga tctgaaagaa atcaatcggg aagcaaaagc ttacttaaaa
     8161 acatcaaccg tagtttttga ggaaaattaa agaaatcttt cggaaaaacg taagaaaaaa
     8221 ataaagaatt tatggtagaa ataaggtaga ctgtataatt tgtccgtagt aaagtaagga
     8281 aacaagaata aatttcaatg aaggaagaga taagatgcga aaagtagcac ctattttgaa
     8341 gccaatcgct gtattaacaa tctcgacgat tgtcgcgact actacaggta ttgtgaatag
     8401 ttccagctac cgtgcaccaa gttcaagttc tagtactgta gaagaaacaa caccaactac
     8461 ttcagtctcg acatctgaac caacaacaac ggaaagtttg aatgaaacca ctacgacaag
     8521 tacatgggac gatacaacaa ctagttcgtc gtatgatgag acatatacaa ctgacagctc
     8581 aagcgaaaca agtaatacgg taccagatgg cactccatcg gattcaacca atccaagtac
     8641 aagtccggat acaagtgcta gctccgaaac gataccaagc agtacgactg tagatgataa
     8701 cacgcaaaat gatgatgcag cacgtacagc acagcaaaac caagattcag gaaataccca
     8761 aacgtataac cagcaagcag gtgattctca atgacatttt tactagacct tatcaacggg
     8821 gtgcataaat tccttggcta tttagacatc agtcctaaat atcttaaccg tggatatacg
     8881 attttaagtg tgattcccac gctttattta ttgagaattg tttatggact ttggcaaaac
     8941 caaaactatc ttcaattttt cttatatgga cttgcatttc tagtgcttgt ttattttact
     9001 gttttaaatg tattttatta ctttttagat aaaaacacga aagccgatgt cactcagctt
     9061 tttgtgaagt atttacctga tgaagcgttc aacatccaga cagaagaatc gatagataat
     9121 ggaaattcga tcgataaagt gaatacacaa gaggtcatgg tttcttatga cgaagattat
     9181 caattgaaat tagccgagaa tatgcggtat ttgattggaa atggtgagat caaaacaaat
     9241 gaactcggtc gcatggatgg atttctggtc gatagaaata cattgtatcc ttattactat
     9301 gtcaagaaaa caggagagaa agagtatgtt ctaaatattg gacgaagcta ctcggacatg
     9361 gaaaaagtag ggaccattcg acttgactca acagaagaca cattggaacc tgtcggattg
     9421 ttcatcgtcg gcggtgattt tgtcaaagac gggttacgct atcatgagcc ttatcgtctg
     9481 aaattgattg caaaaaaggc acagtcgcag gaagaactag tggtttatag tcgtagccaa
     9541 agaagaagac attcataatg tgatgaaacg ccgtgtatca gtggttgcga taaaaacgtt
     9601 tattcggaat aagggggatg tgacacgtct tttgtcacgt tcctttttta tataacgaga
     9661 aagataagac gaacacatca cttattcttt cctttcattt ataaacattg tagaataaag
     9721 aaagtacaac gaagtattgt gaaaaaacgt aataaataac gaggaaaata caagtaggag
     9781 gtaagctgtg aaagaatttt tgaaaaattg ggggattttg atccttgtat tagcggctat
     9841 tttattagct agagtgtacg tgtttacacc agttactgta aacgggcatt cgatggaccc
     9901 tacattaagt gatggacaac gattgatttc atctaagatc tcaaactatg aacgaatgga
     9961 tattattact acaaaggaac cgggggatga agaacggatg attgttaaac ggatcatcgg
    10021 tatgcctgga gatacagtca agatggaaaa tgaccagttg accattaatg gcaaaaagta
    10081 tgatgaacct tatctggatg agttcaaaaa agaattttca gaagataagt tacaaggaga
    10141 atatgcttat agctcaggat tccaagcaca agcagaaagt tcatccacct ttacgagtga
    10201 ctttgaatat actgtgccaa aaggaaagta tcttgttcta ggcgataacc gcttgatttc
    10261 aaaagacagc cgaatgtttg ggttagtcga caaagatatg atccaaggaa aagttgtttt
    10321 ccgttattgg cctctttcag aaattaaaat catgtgatga aaaaataaaa agaagctgtg
    10381 acatgttttt tgtcacagct tctttttccg aaaattcctt cttatttctt ggagttgctc
    10441 actttttttg caatctcatt ttaagctttt tggctgatgg ataagaaaag cgaagcgttt
    10501 ttgttcataa atagcttttc cccacgatgg aaggtatgac tagtagcaga gacaaagccc
    10561 gttttttcca atcgattatt caattcttct tggctaaagc cattatggac tttcggatga
    10621 ctaatttgtt cattcttgtc aaagtctacg atgatcaatt gaccgccagg agctaaaatt
    10681 tcataaagtt ttgtaagaat attctccgta tcaggaatgt gtaaaagtac aagtgataga
    10741 aggatgagat tagcttggac agcaggaaat tcgacagaag catcagcgtg gatcgttcgg
    10801 acatttttca gatctgctgc ttggatcttt tcttctgcca ttttcagcat agtttctgaa
    10861 gcatcggcaa tgatcagttc tttgaatctt tccgcaagcg gtaaactaac aagcccagtt
    10921 ccgcctccgt aatccagcag gactttttgg tggctgtcgc gcggtagata agtagttagt
    10981 tcttcgcgaa taatcttggc taatgcttga cggtcagggg aatcataatg gttagcgatg
    11041 gaatcaaaaa tattttttgt catcaaatag ctccttacgt aaaactttga aattttaact
    11101 tattatagca cgtgaaaaga aagaaagcat gctatactca attcaaaaag ggagaatcta
    11161 gaaagaatat gccgatatcg tttgtaaaag atagagagga aaaaggaaag tgtgtccgag
    11221 aaatattact agacttgcct gagtggtttg ggcttcctga atcaacggaa aaatatatcg
    11281 aggaatcaag taaactacct ttatggtgtg aaaaaagaaa agaagaatat ttaggcttta
    11341 tcactctttc acagacaagt gaagatactg ccgaaatcta ttctattgta tgggaataaa
    11401 aaaagactat catcaccaag gattaggaac aaaacttttt aaagaagcag aaggttacgc
    11461 agcaaaacat tacaaatatt tgcaagtgaa gactgtagac gaggggcact actctatata
    11521 tgatcaaact atttgtttct acgaatcatt aggattttca aggttggaag tttttccaaa
    11581 tttatgggat gaatggaacc catgtttagt tcttgtcaaa aaattggaac aaaagtgagc
    11641 aatagattta atactaatct gtgcttgatg aaaattctca ctgtaaaaaa tgctgaaaat
    11701 caagtataat accataggaa caaactggaa ggtgaaaaag agcatggcac aactattttt
    11761 taagtatggc gcaatgaata gcggcaagac gatcgagatc ctaaaagtcg cacataatta
    11821 tgaagaacaa aataagccag ttgtcttaat gacaagcggt attgatacaa gagatggttt
    11881 aggcgttgtt tcaagccgaa tcggactgaa acgtaatgcg atgccgatat ttgaagaaac
    11941 agacgtattt gacgtaattc agaaaatgga aagcaaaccc tattgtgtgc tgatcgatga
    12001 agcgcaattc ttaaaaaaga aacatgtcat tgcttttacg agaattgtgg acgaattgga
    12061 tattccggtt atggcatttg gtttgaaaaa tgattttcga aacgagttat ttgaaggatc
    12121 aaaatattta ttgctttatg cagacaaaat cgaagaaatg aaaacaatct gttggttctg
    12181 ccacaaaaaa gcaatcatga atttacatta tattgatgga aaccctgttt atgaaggcga
    12241 tcaagtgcaa attggcggaa atgaagctta ttaccccgtt tgtcgaaaac attactttca
    12301 cccatcaatg attacagaag gagaataagc atgtacgatc aattacaagc aattgaagac
    12361 cgttatgaag agcttggaga acttttaagt gacccagaag tgatttctga tacaaagcgt
    12421 tttatgcagc tttcaaaaga agaagcaaac acgcgcgaaa cagtcgaagt ttatcgtcgt
    12481 tataaagaag tagtacaagg aatcaccgat acagaagaac tgcttggtga aaaactggat
    12541 gatgaaatgg ctgaactagc aaaagaagaa ttatctgaat taaaaaaaga aaaagaagtc
    12601 ttagaagaaa aaatcaagat cttgctttta ccaaaagatc caaatgacga taaaaatatc
    12661 atcatggaaa tccgtggagc agcaggcgga gacgaagcag cattattcgc tggcgatctt
    12721 ttcaatatgt accaaaagta tgctgaagca caaggctgga aaacagaagt gatggaagct
    12781 aacgttaccg gaatcggtgg atataaagaa gtgatcatga tgatcaccgg agacaacgta
    12841 tattcgaaat taaaatatga aagtggtgcg caccgcgtcc agcgtgttcc ttcaactgaa
    12901 tcgcaagggc gtatccatac ttccaccgca acagttgtcg tgatgccaga agctgaagaa
    12961 gtagagatcg atattgaaga caaagatatc cgtacagata tctatcatgc ttcaggtgct
    13021 ggcggacagc acgtcaataa aacagcatct gctgtacgtt tgactcactt accaacaggg
    13081 atcgttgttg ctatgcaaga tgaacgttct caaatcaaga accgtgaaaa agcaatgaaa
    13141 atcttgcgtg ctcgtgtgta cgataagatc cagcaagaag cacaaagcga gtatgatgcc
    13201 aatcgaaaat cagcagtcgg aactggggat cgttcagaac ggatccggac gtataatttc
    13261 ccgcaaaatc gtgtgactga tcatcgtatc ggattaacta tccaaaaatt agatcagatt
    13321 ttagcaggga aattagatga gatcgttgat gcattgatca tgtatgatca aacatcaaaa
    13381 ctggaagcta tgcaaaatgg gtaaaatgac ctatcgagaa gtcctgtcac gggcttcttc
    13441 ttttttagag gatcatggga aagaagcata tagtatccaa tttttatttt tggaacgaaa
    13501 acaatggaaa aaacttgatt ggctccttca tatgaacgaa gagatttcag aagaagaaca
    13561 gcggttgatc gaaaccgatc tgcgacttct gcttgcagat catccacctc agtatttgct
    13621 aggatatgca gatttttatg atcatcggtt aaaggtaact gaagcaacac tgatccctcg
    13681 accagaaaca gaggagttag tcgaatggtg tttagacgag acgcctggtg taccgttaga
    13741 agtgatagat attgggacag ggactggagc aattgctatt agtttgaaag cagcaagaaa
    13801 aaattggcat atatctgctg tggatctttc agaagaagca ttagaagtag cgaaagaaaa
    13861 tgctcagaaa gaaggaacga aaatcagttt ttatcatgga gatacattag gaccagtaat
    13921 ggatcagcaa tttgatgtga ttatttctaa tccgccgtac atcagtcgga atgaatggga
    13981 actgatggat gaaagtgtcc ggtcattcga acctaaaatg gccctttttg ctgagaacga
    14041 tggattagct atttatgaaa aaatagcagt tgaagcacca agcgtactaa aatcaaatgg
    14101 aaagattttt ttagaaatcg gatttcgcca aggtgaagca gtaaaaaaaa tatttcagca
    14161 agcatttcca gataaaaaag tggcgataaa aaaggatctt tttggcaatg aacgaatgat
    14221 ccaagtttat ggttgaagtt gtggatgaaa aattttttat ttgttttttt attataaaaa
    14281 aacacttatt tgacaaggat gtaaacgact ttttggttta tcaacaagtt tatccactat
    14341 tgacaaatag attatccaca aacagtggaa aacttgagga taaactcaga caaaataaga
    14401 atgtatgaaa aaggggttgt ggataaactg tggaaacgaa acgatatgat gaaacagaat
    14461 tgcaagaggc agccgaagca ctgaaagaag gagaacttgt tgcttttcct accgaaacag
    14521 tatacggatt gggcgccaac gctcttttac caaacgctgt caagaaagtg ttttctgtaa
    14581 aagggagacc gcaagataat ccattaatcg tccatgtcgc ctcttttgaa caagtaaaag
    14641 agtatgtgga caactttcat ccggcagcag agaagattgt gaagaacttc tggcctggac
    14701 cattaacttt gatatttaag atcaaaaaag acactttgcc ctcagttgtg acgggaggat
    14761 tatccacagc tgcatttcgt ataccggata acaaaaaaac actagaagta atcgaacttt
    14821 ccggtgttcc gcttgtcggt ccaagtgcaa atacgtcggg aaaaccaagt ccaacgactg
    14881 cagatcatgt atatcatgat ctacaaggaa aaattaccgg gattattgat gatggagcta
    14941 cgcgaattgg tgtagaatcc actgttcttg atttaagtga tccaacagcc atgcctatga
    15001 ttttacgacc aggtgcagta acgaaagaac aaatcgaagc agtcatcgaa tctcctgttg
    15061 ctattgatca gcatcttgta aaagaaaatg aaacaccaaa agcccctggg atgaaataca
    15121 aacactactc acccgataca cgtgttctga tggtcagaga tggcgattgg tcaactgcag
    15181 ttcaatgggc aaaaaataag aagatccgtg caggtgtcat agcaagcccc gaaattgcgg
    15241 atcaagtgcg gacagatact gcagctgttt atatgtataa cgacaactca gtagaagcgg
    15301 cggctaaagg attgtttgca ggtctaagag gattagatga accaacactt ggattagatt
    15361 tgatttttgt acaagtgtat ccagaaactg gattggggaa tgcttacatg aatcgcttga
    15421 aaaaagcagc aggacaaaat tattttgaaa aatgatcaat tggttattgt cgcattcttt
    15481 tagtatgata caatgctatc aagtaaatct tctatcagtg aacgaaaaac taagcgtaac
    15541 taatcgtaat agaagagata cgaatatttg gcaatttgtt tttgatggga aaaataaaat
    15601 tgtaggaaaa taaaaaggag ctgttgtttg tggtagatta caaaacgttt gaccctgatc
    15661 tttgggctgc gatcgccaaa gaagaagaaa gacaagaaca taatttggag ctgattgcct
    15721 ctgaaaactt tgtctccgaa gcagtgatgg ctgcacaagg aagtatcttg acaaataaat
    15781 acgcagaagg ttatccaggg catcgctatt atggtgggtg tgaatttgtc gatatcgttg
    15841 aaaatttagc gattgaccgt gcaaaagaat tattcggagc gaaatttgcg aacgtccagc
    15901 ctcactccgg ctctcaagcc aacacggctg cttatctcgc tttggtagaa ccaggggaca
    15961 cgatcttagg aatggatctt tccgcaggcg gacatctaac acatggatca ccagtcaact
    16021 tcagtggcaa aacctatcac tttgttgctt acggtgttga tccgacaaca gaagttattg
    16081 attataatgt ggtacgtatt ttagctagaa aacatcaacc aaaattgatc gtagcaggtg
    16141 ccagtgctta cggacgaacg atcgactttg cgaaattcag agaaatcgca gatgaagtag
    16201 gcgcaaaact gatggtagat atggcacata tcgcaggatt agtagcagca ggacttcatc
    16261 ctaatccagt tccttatgca gatatcacaa cgactaccac tcataaaaca ttgcgtggcc
    16321 ctcgaggcgg catgattttg acaaacgatg aagcattagc aaaaaaaata aatagtgcgg
    16381 tatttcctgg tattcaaggc ggccctctag agcatgtgat tgcgggtaaa gcagtagcat
    16441 tcaaagaagc attagatcct gcatttaaag aatatagtga acaaatcatc gccaatgcaa
    16501 aagcaatggt caaagttttc aatcaagcaa ttggaaccag agtcatttca ggggcgacag
    16561 ataatcatct gatgttgatc gatgttcgcg agttaggtat aaatggaaaa gaagcagaga
    16621 gcattttgga cagtgtgaat atcactgtaa ataaaaactc gattcctttt gaaacactaa
    16681 gtccattcaa aacaagcggt attcgaatcg gcacgccagc aatcactact cgcgggttca
    16741 aagaagaaga tgctgtaaaa gtggctgaac tagttgtcaa agcattacaa gcaaaagatg
    16801 ataacgctca actagacgaa gtcaaaacag gtgtacgcga attaactgaa aaatttccgt
    16861 tgcataaaaa ataaaaattt aaattccatt agtaaacaga aaagttcaat gttgggcttt
    16921 tctgtttatt ttttgggaaa acggattctt tgacaatatc ggtgatgctc tctagtatat
    16981 gaggtgtaat tttagtcttc acactaaatc tcagggagga aaagtcatga ctgatcgtat
    17041 ctcgttagaa aaggaagcag tagcattcag tgatgcaaat gcgcctcatc cgcgtatcta
    17101 tgaactagca ccggaagaag gaagagtttt actagaagaa gtacaagaat cacctgtgaa
    17161 caaatattcc gtcgatattg aagacttgat ggtggatact ggcgaatggg gaaaactcaa
    17221 tattcgtctc gtacgaccag aagggaatat ggataaatta ccagttattt tttatattca
    17281 tggagcaggc tgggtttttg gcagtgctca aacccatgat aaattgatca gagaacttgc
    17341 ggttcgtacg aattccgtcg ttgtctttcc agaatatagt cgatcaccag aagcaaaata
    17401 tccaacagca attgaacaaa attatgctgt gttgcaacag ctgaataaat tagccaagtc
    17461 aaaaaatctg gatctcgaga atctaatcgt tgcaggagat tcagtaggtg gtaacatggc
    17521 tacagtcatg acgatcttaa caaaagaacg aaagggcctc ccaatatcca agcaattgct
    17581 gtattatcca gtcacaaacg ctgattttga tacagattcc tatcatgaat tcggagaaaa
    17641 ctattttttg acaagagaag gaatgatgtg gttttgggac caatatacga cgaatgaaga
    17701 agagcgtgct gaaatcacag cttcgccttt gagagcaaat ataaatgaac taaatgatct
    17761 tccttcagca atgattttaa cgggagaagc ggatgtatta agagatgaag gtgaggctta
    17821 tgcgcgcaaa cttcgtgatg caggggttcc tgttacacaa gtacgcttcc aaggaatcat
    17881 ccatgatttt gtcatggtaa atgctatgga tcagacaaag gcaacgcgtg cagcaatggc
    17941 attatctact tcttggatca agaaaaaaga ataagctttg cttgtccgca ggaaacttct
    18001 tttcaaaaac ggattttctt aggaaaaatg gcgagtttga ctagtcaatc acttgatttt
    18061 gttttacaat taaaaagaaa cgacagaaca aaggagagct taaaaaatgg gcaaatttca
    18121 agtgatcgat catccattga ttcaacacaa actaactatt attcgggaca aaaactgcgg
    18181 aacaaaggtt tttcgtgaag tagtcgatga aatcgctatg ttgatggcat atgaagtatc
    18241 acgggatatg ccattggaag acatcgtgat cgaaacaccg atcactgaaa caacacaaaa
    18301 aacgttgtct ggtaaaaaag tagcgattat tccaatcttg cgcgcaggta ttggaatggt
    18361 tgacggtatc ttggaattga ttccagctgc taaagtggga catatcggtc tttatcgaga
    18421 tgaagaaaca ttggaaccac atgaatattt cgtcaaactt ccagaagaca ttgatgcacg
    18481 tcagttgttt gtagtagatc caatgttggc aacaggcgga tcagcaatca tggcaatcga
    18541 tgcattgaaa gaacgtggcg cgtcgaacat taaatttgtt tgtttggtag cagcacctga
    18601 gggagtaaaa gctttgcaag aagcgcatcc tgatattgac atctatacag cttctctaga
    18661 tgaaaaattg aatgaacatg gttatatcgt tcctggacta ggggatgcgg gtgaccgttt
    18721 gtttggtaca aaataagtaa atacgaaaaa gggtgtgaca caacttttgt cacatccttt
    18781 tttggtcatt tttaacgttt gatgcagaac agctttttca cagctttcgt ttgcatataa
    18841 aaaaaccatc ccaaaaatgc agagatggtc aaattcaaat cttttttagt tctttgtagt
    18901 taacgattac tacaccctta ctttgcgtca tcaagttcgc attttctgca gtttcatcaa
    18961 tctcaacaac agcggaattt ttcaattgct ttgaaatcgt tccagtttgc gacttgccgt
    19021 gaattgtgaa cattacgtga gtattgatct tgaatttttc accaatttcc gatggtacga
    19081 tagttgtatc aaattttcca aaagttgcca ttttcataaa acctgccttt cacatcaatt
    19141 ataccacgtt ttatgaaaaa tatcatgaaa ttaaactaat tttccatcgg actctttaca
    19201 atcagccaaa tagagtatat aatttctttt ggataaggcc agcttagctc agttggtaga
    19261 gcaacgcact cgtaacgcgt aggtcgtagg ttcgatccct atagctggca ttaaaagaga
    19321 agacagatat atcaaggatt tttaatccat tgatacatct gtctttttgt ttaaaggaaa
    19381 taaagtatgc tttcttagca atagagcatg tattatttct ataaaaattt aggagaataa
    19441 aacgactagc aatatagctg tgaaatcaaa tatcacatga actgcccaac ttgtaatgat
    19501 cgagttgctt ttaatggcag cttggttgaa taacaatctt gccacccctt gtatcaaaag
    19561 gatgtgtgct acagtgtgta aaatatttcc attatcataa gtactgtaat ggataaatcc
    19621 aaagatgatt gctgacaaga aactagcgac aggaatgctg aacttactag aaaaaatagc
    19681 gagaaaatac actgagaaca attcttctcc aagtatcatg ataggcaaaa taataaataa
    19741 taacgggctg aaagcctcat ttattggatt gcctgttaaa ccccatttca aaatttgttg
    19801 caaaaatacc gagactgcgg cactgattac tacattgata aaaaaatatt tggctatatt
    19861 tttgacagga gcgacaggtt tcgagaacat ttttttcgta tcttttttac caaaaacaag
    19921 tgtaaatgct ataaatccag tgatagttaa agaaaaaatg ctaataatgg atggcgcaaa
    19981 tagcaaccct aacaaaccga atacaacgac aatagtgccc aatatccctt ttttaagttc
    20041 tatttttttt cctaaaatgt tattcatgat aataactcct actattcagt ttttg
//
LOCUS       DAGCYL010000033        20033 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.034, whole genome shotgun sequence.
ACCESSION   DAGCYL010000033 DAGCYL010000000
VERSION     DAGCYL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.034"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..226
                     /locus_tag="MB216_000932"
     CDS             <1..226
                     /locus_tag="MB216_000932"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBT4525787.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="MB216_000933"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="MB216_000933"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="MB216_000934"
     CDS             533..1336
                     /locus_tag="MB216_000934"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBT4525788.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1585..2649)
                     /locus_tag="MB216_000935"
     CDS             complement(1585..2649)
                     /locus_tag="MB216_000935"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBT4525789.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(2646..3731)
                     /locus_tag="MB216_000936"
     CDS             complement(2646..3731)
                     /locus_tag="MB216_000936"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBT4525790.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="MB216_000937"
     CDS             complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="MB216_000937"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBT4525791.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="MB216_000938"
     CDS             complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="MB216_000938"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBT4525792.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5974..6633
                     /locus_tag="MB216_000939"
     CDS             5974..6633
                     /locus_tag="MB216_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBT4525793.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            6718..7623
                     /locus_tag="MB216_000940"
     CDS             6718..7623
                     /locus_tag="MB216_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBT4525794.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(7625..8257)
                     /locus_tag="MB216_000941"
     CDS             complement(7625..8257)
                     /locus_tag="MB216_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBT4525795.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    8408..8533
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            8577..9101
                     /gene="infC"
                     /locus_tag="MB216_000942"
     CDS             8577..9101
                     /gene="infC"
                     /locus_tag="MB216_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBT4525796.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            9173..9373
                     /gene="rpmI"
                     /locus_tag="MB216_000943"
     CDS             9173..9373
                     /gene="rpmI"
                     /locus_tag="MB216_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBT4525797.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            9426..9785
                     /gene="rplT"
                     /locus_tag="MB216_000944"
     CDS             9426..9785
                     /gene="rplT"
                     /locus_tag="MB216_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBT4525798.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            10221..10562
                     /locus_tag="MB216_000945"
     CDS             10221..10562
                     /locus_tag="MB216_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525799.1"
                     /translation="MLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLIRKV
                     VELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVKSTILCKS
                     STSSKAQCFAL"
     gene            complement(10669..11856)
                     /locus_tag="MB216_000946"
     CDS             complement(10669..11856)
                     /locus_tag="MB216_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBT4525800.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            11953..12849
                     /locus_tag="MB216_000947"
     CDS             11953..12849
                     /locus_tag="MB216_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBT4525801.1"
                     /translation="MDVIGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAA
                     ESYTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAV
                     LLPVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMV
                     GFLMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSG
                     SAILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKS
                     IPTGWIIGAILCLITFLLYGNA"
     gene            12897..15026
                     /locus_tag="MB216_000948"
     CDS             12897..15026
                     /locus_tag="MB216_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBT4525802.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIESVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            15001..15690
                     /locus_tag="MB216_000949"
     CDS             15001..15690
                     /locus_tag="MB216_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525803.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(16098..16784)
                     /locus_tag="MB216_000950"
     CDS             complement(16098..16784)
                     /locus_tag="MB216_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4525804.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(16920..17114)
                     /locus_tag="MB216_000951"
     CDS             complement(16920..17114)
                     /locus_tag="MB216_000951"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525805.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(17164..17604)
                     /locus_tag="MB216_000952"
     CDS             complement(17164..17604)
                     /locus_tag="MB216_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBT4525806.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(17608..18453)
                     /locus_tag="MB216_000953"
     CDS             complement(17608..18453)
                     /locus_tag="MB216_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBT4525807.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            18602..20020
                     /gene="pepV"
                     /locus_tag="MB216_000954"
     CDS             18602..20020
                     /gene="pepV"
                     /locus_tag="MB216_000954"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBT4525808.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         6071 a         3841 c         4191 g         5930 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggacagaagc gtttaactcc aagaaataag aaggaattca cgaaaattgc ttttcaaatt
     1501 tttgtgaatt tcagcttatt tccgaaggag ttgcttttgc ttccgccgtt tatacgtgtt
     1561 tagagcgtgg gataaaagtg ttctttactt ttgtcccacg ctcttttctg tgcgtcttcc
     1621 atacttcact aagtcgatcc ctcttgcttc gcaccaatcc ttcactggtc cggaaaagat
     1681 cagtgattgt tggtgtaggg ccgcggtatt cgttgcgccg accatagtat aaagcagacg
     1741 aagctcttct tgccattgct tcatcagtat aatagtttct tctacgccgt gattcatcag
     1801 atgcgtcagt acggttcctg aagtacccac tgcatttgcg ccgagacaaa gggctttaaa
     1861 tatgtcatag gcattgcgga tacctccgga ggctaggatt tccatagaag tgtctgcttc
     1921 atttgcttca agcaatgaag agactgtcga ttgcccccag tcagctaaat aactcagttc
     1981 ccgcttggag cgacgggcgt tttcgatctg ggtgaagctt gttccgcttc gaccgctgat
     2041 atcaactgta tgaactccta aagcagctag atcattgagc gtttctcttg tcatgccaaa
     2101 accgacttct tttacgatga gggggacgtc aatggctgtt tgagtctctt gaataagtac
     2161 tttccaattg gtaaaatcac ggtctccctc aggcataacc aactcttgag gtgcattcaa
     2221 atgaatctgt aaggcatctg cgtgaaacaa tcggatagct tcttgggctc tttcgacaga
     2281 agtccctgca ccgatgttgg caatgatttt cccgtgagga tattcttgtc gcataatcgt
     2341 ataagtatcg gctaaagaag ggtctttcaa ggcagcgctg actgaaccag tggcaatcat
     2401 caaatctgct tctcttgcga cgattgcaag atcttggttg attttttttg ttttttcgct
     2461 tccgccagtc atcgcattga tataaaatgg gctgcttagt gtaaacccaa ctgcctgtgt
     2521 acttagatcg acatcagaaa ctgctatttg cggcaaaggg ttatggatga tccgtacgaa
     2581 gtcaaattcg tttttttgtt tatcatgaaa ggctttagct agtgaaacat gttcgtcttt
     2641 tcgattcatc ggttttcctt tctttgatcg ctatagacat gtaacggcag tggagtgatt
     2701 tctgcttttt cccatgcact cattaaagga aggatgccag atttctggtc aacaattacg
     2761 attccgcaat cgccaccacc tgcaccagaa gattttgcgg ctccttcata ctgttcagct
     2821 aaattacaca atttgttcaa agcaggcgtt tcgatgacga ccccagtgat tgcagataaa
     2881 tcatgcagta attgtcggtt ttttcgaatc atctgttgga tcaacgttac attattttct
     2941 ttaaaccctt tgatcatttc gttgacacat tctgtactgt tttttaagaa aagctgataa
     3001 gccaccattt tgtcttctct cgaacggtga acttgatcga ccaaatcaga agtagaggca
     3061 gggctacccg tccaaccaat caataaacgt aaatcttcag gagcaatcaa tggctcaatg
     3121 gatagacctg gccaatccag ggcaagtaac tcactgatag aatgctgagt ttcttgttct
     3181 tggagccaag gatggtcgaa ggttgagaaa gcgatccagc caccataaca gctagcagcg
     3241 atgtcgccgc aggaaccatt atcttgaact gctaaattgg ctagtgctgc aatcttgaaa
     3301 atctccaatt gagataaatt caacgcgtaa aaaacattca aggctttgac cgttgcaacg
     3361 gtcacggcac cgctagaacc caatccatat ttccggccat ttgaactgtc taactcactt
     3421 gttactttca gatcataaaa ggataaaaga atgttttttt cttgtgcata cttttcagtc
     3481 aaacgaatag cagcaaggat ataatgaaaa ggattttccc gaatatctaa aactaattct
     3541 ccgttgcgtc ttgtccaacg tacaggcatc ccactatatt gtgcagattg gatacttccg
     3601 acttttcgtg cggattctac agtgactgtt acgaattgat cgactgcagc gataactgct
     3661 ggatgtcctg tttcaacaac tgcatattct ccggcaatat agagtttgcc tggtgcagat
     3721 acttcaatca taaaaatctc cctttattca ataatcgcaa ttcctggacc agcatgggca
     3781 gtaattactt gttctttgct aaataatttg gtaaatgttt cttgtacctc agataaatgg
     3841 ctgttttcca ctaaaacttt gacattaggg ccagcatcca tcgtaaagta gcatggaatg
     3901 cctttttttc tcaattgccg caccgcatcc atcgctttta agctgtcagg agaccagtaa
     3961 gtgaatggag gctgggcagc taaagtcgtt ccatgcattt ttagtccgtt tctttccatc
     4021 gtttctccga gaagttggaa atcttttgtt ttgattgctt gtttcaattg atatagatcc
     4081 ccttcaacgg aatctaacca gccttgataa aaactggatg tttcgactgt tcgacgcatc
     4141 ccatttctgc tggacacttc ttttttctgg tcgttgatca aaacgaaaac cattgctaaa
     4201 tcgtcttcga aagaatcgga agggactggc ttagcgtaag aacttaagtc gtcatggcct
     4261 ttttcccatt caacgaaacc accgaaaata cttcggcaag ctgaaccaga cccgcgtcga
     4321 gcaaatcttg aaagagagag atcgtctaat ccaagcttaa gagcagtatt gcaagctcct
     4381 gctaaagcag ctagcccgct ggcagaggag gctaatccag ctgcggtagg aacgaaattc
     4441 tgactgatta ctgaagcttt tagtgagagg ccggcttctt tacgaaaaag gtcaagaaac
     4501 tgactgactt tttttgttgc tttttcgtct tgtaaggtgc catctagata aaattcatct
     4561 accatatagc tatcagaaaa gatgacttct gtctctgtat aaaatgcatc caatgttaac
     4621 gaaaggctgt tgttcattgg aaggatgagt tcttcatttt tcttacccca atatttgatt
     4681 agagcaatgt tggtatatgc gcgtgctttg cctttaaaca taggtatgta ctcctaatcc
     4741 ttgtatccaa gtttcagcag cacccgcatc ttcaagtgct tgactgattt cttgggcttt
     4801 tgtttttgtt tgtgctaatg caatcataca gccaccgcgt ccaccgccag ttaatttggc
     4861 acccaatgcc ccggtatctt ttgctgtttg gatcaataga ttaagaaaat cattgctgat
     4921 tgtcagcttt tccagaagtg actgtgattc atccattgtc tgtgccaacg tttctggtga
     4981 attctcaatg attgcttgtt ttgcttgctt cgtcaagtat cctaattttt ggatcatttg
     5041 tccagtttca tgaggctggg tttcaaagag gtgagcgaca tctttgacgg cttctcttgt
     5101 ttgtcctttg attcctgtat cagcgacaat caaaaaagca tcgatgttca aagaaaagta
     5161 gtcaaaagga tgcccttttg taaaataaat cggctgcaag ctgctagtag cggctgcatc
     5221 gattccacta ggattaccgt gggcgatttt ttccgaaagc tggacatttt ctaatagtat
     5281 ttcacgagac aaaggaaatg ctaagtaatc ataaaaagca cgagtgactg ctgtggcgac
     5341 tgcagcgctt gatcccattc cacgttcagc aggaatcgtg ctttcaatgg tcagttgcaa
     5401 gtttttttgg atgtcatgct gtttttttaa gtgaatgaat aattttttga tattttttaa
     5461 tgcatgagga gcttcggcta aatttcctga atagtaggaa gaaaccagtt gatcgtccat
     5521 tgcatccagc tcttcaagga atgcggtgac ttttgttgca tagaaaggaa aggcaatcgc
     5581 tggttctcca taaacaaccg cgtgctcgcc catcaatatg atctttccgc ttgattctcc
     5641 ttggccatag tttgccataa tctaactcct tctcacgtaa aagtgatttt taagcaaaaa
     5701 ataaagcgcc caaaacgagt tgcgctgctt cacgaatttt tttgtccact aagggtctca
     5761 aaaaacattc ctcctttatt ttactaaaac catctcagaa gtaaaactaa gaatacgttt
     5821 tctaaagaaa aaacactttt ttcttaagaa ttttttgact ttttttcttc tttgactaga
     5881 aaaaatacaa ttgacactac tagatattgg gggtatattt aaaaaaggtt ggagttaaac
     5941 acaatattta gtgatgaagt gagctggagg taaatgagcg tgatcgtttt agcaggtaca
     6001 attggtgccg gaaaatcaag tttaacagaa atgatggctg aacactttga tagtcaggca
     6061 ttttatgaat cgattgatga taatgaagtc ttgcccttat tttatgcaaa ccctgaacag
     6121 tatgcttttt tgctgcaaat ttattttttg aataaacgtt ttgcaagcat caaacaagca
     6181 atgaaagatg acaataatgt attggaccgt tcgatttatg aagattcact gcttttccat
     6241 ctaaatgcag atctaggaag agcaacagaa acagaagttt gtgtgtatga tgaactgctg
     6301 gaaaacatga tggaagaact tccctatgct gcccataaaa aacatccgga tttattagtt
     6361 catattcgtg tatcttttga tacgatgtta gagcgtatcg aaaaacgtgg tcgttcgtat
     6421 gaacaattgt cctttgaccc aagtctatac gattattaca aagaattgaa cagacgttat
     6481 gatcaatggt atgaagaata caaagaaagt ccaaaaatcc aaatcgatgg agaccgctat
     6541 aattttgtgg aagatcctca agcaaaagaa gaagtcttga aaatgatcga agaaaaatta
     6601 gcagagattc gccaaaccaa agtatccatt tgatttacag ttgctcaaat aaagggtaaa
     6661 cctttatttg agcgactttt ttctttatac tggaaacaaa tgtcaggagg atagagtatg
     6721 cgggaaaaac actattttaa tcaagaccgt aatttttgga tcaatgtaca gcaaaacgat
     6781 gtgctagctg ttaaaaactt aaaagaagaa tatggcatta gtgacgagat gctgacttat
     6841 tctctagata aaaatgaacg ggcgcgcgta gagtacgatt cttttgatga agcgttactg
     6901 cttgtatcaa atgttccgca ccagcaaaaa gtcgataatc attatgaaac tagtcctatc
     6961 gcttttattt taaaagaaga cggtttgttt acatttacca cgccgaatac cgagtacgtg
     7021 attcgactaa ttcgatcgct tttggaacgg atgccagaca tgagtgtata cagtttattg
     7081 tttcgtacat tgtttttgat ttctgattcc ttttttcctt taatcgagga agtgaatagt
     7141 gaacgtcagc ggcttaatct gaaattaaga gaaaaaacaa cgaacaagaa tctgctgcaa
     7201 ctttcagatt tagagatcgg tttggtttac ttagttacag gaacaaaaca aaatgtagta
     7261 cttttagaac aaatcaaggc actggctatt tatcgcaagt tgtcagaaaa ggaaaaagaa
     7321 gagttggacg atgcactgat cgaagcacga caagcagttg aaatgacgaa cttagcttcg
     7381 cagattttag accagctctc tggcacttat aataatttgc tgaataacaa cttgaatgac
     7441 acgatgaaat ttttgacggt ctggtcgctg atcttgaccg ttccaactat tgtgacagga
     7501 ttcttcggaa tgaatctcca gttacccttt acccattccg ttttcggatg ggaaatcgcc
     7561 ttgatcatca gtttggtctt atctatttgg atgcttattg cactgtggcg gcggattcgt
     7621 tgatctagtc tttgatctgc aaaccaaggg cttgggcgat cacaagtgct tgctcttgat
     7681 tgacgattag tcccgccaaa gaatcttgag ataaagcgat tgttgtgaag aaattagtcg
     7741 taaagtctaa tcccttcaga gaagttcgaa accagttcga accggttaac tgattgtcag
     7801 acaaaaaaag gtttttccaa gtgacctcat aaaattcgct gtcttctaat tgacaatgat
     7861 cgaaggagac agctttcaag tttgtactac taaaggaagc catcgaagca atacaatcag
     7921 tgaaagtaca gtctcgaaga taactttcgg caaaattcgt acctgttaat ttacattgat
     7981 gaaacacgac ttggtggaaa ctgccaccaa tccactccag attggaaaaa tcacattttt
     8041 caaaaatgac attactgcat tcaaaatgac gtaaacctgt tttttgcatc gtaagttttt
     8101 ggatatgact ctctcttaat accatatttt tggcatcgag atagctttgg ttacctccgt
     8161 tgatcagaag accagagaag atctcctcat cttctaaata aaactctccc tttgtaagaa
     8221 gaggaagaga aggtggaata ggttcgattt ttttcatgtg ggttcacctc gtttttcttt
     8281 agtatagcaa agaagcggga atcactgcta gaaagcctgc taatctaatc attttcagaa
     8341 aaggaagaca aactgtatga aaaacattga caaaaagatt agtttttgct atactataat
     8401 ggttgagaaa taaaagcaga agcacccgct tctcgcctta gttgataatg tcactgggct
     8461 agatagtttt cactggttct attaggtgaa gtacgcgcgg gttctgtaag agaactcgtt
     8521 tttttattgc cgtttattcg gtaagctttt ctttctctca taatatttgg aggtgaatga
     8581 ccatagcaaa ggatatgatg gttaacgacg gcattcgtgc acgagagtta cgattgatcg
     8641 gttcagatgg tgagcaatta ggagttaaga caaaagcaga agcattgaat attgcagaac
     8701 aagcaaactt ggatcttgtg ttagttgccc ctggtgcgaa accaccagtt gcgcgaatca
     8761 tggactacgg taaataccgt ttcgaacaac aaaagaaaga ccgcgaagcg cgtaaaaaac
     8821 aaaaagtgat caatgtaaaa gaagttcgct taagtcctac gatcgacttg aatgacttca
     8881 atacaaaact tcgtaatgca cgtaagttct tggaaaaggg cgataaagtg aaagcttcta
     8941 tccgtttcaa aggccgtgcc attacccata aagaaattgg tcaaaaagtc ttgaatcgct
     9001 tagctgaaga aactgcggat attgcaacag tggaacaaaa agcgaaaatg gacggacgca
     9061 gcatgttctt gacgctggca ccgaaaaacg acagtaagta atttgaatga ctaactggct
     9121 gttggacagc cgatagtcgg ataacgaaag attttgagga ggaaatatag tcatgccaaa
     9181 acaaaaaaca caccgcggat cagcaaaacg tttcaaacgt actggtaaag gcggattgaa
     9241 acgcttccgc gcgtttacaa gtcaccgttt ccacggcaaa acaaaaaaac aacgccgtca
     9301 attgcgtaaa gcaagcatgg tttctagtgg cgacttcaaa cgtattcgcc aacaactagc
     9361 aaaaatgaaa taagaagtac aaataacaga atctgaagat ttatttttat taggaggaat
     9421 tatacatggc acgtgttaaa ggtggaacag taactcgtaa acgtcgtaaa agagtgctaa
     9481 aattagcaaa aggttactac ggttcaaaac acactttatt taaaacagca aaagaacaag
     9541 taatgaattc atacaactac gcatatcgcg atcgtcgtca aaagaaacgc gacttccgta
     9601 aattgtggat tgctcgtatc aacgcagcag ctcgtatgaa tggcttgagc tactcaaaat
     9661 taatgcacgg tttgaaagtt gctgaaatcg acatcaaccg taaaatgtta gctgacttgg
     9721 ctgttaacga tgcagcagca tttactgcat tggctgacca agcaaaagaa gctttggcta
     9781 aataagctaa acaattataa agaggaaaga agataacgaa aagttatgtt ctttcctctt
     9841 tttttattta caatcctcca tcaaatgaaa caggacaata acgtataagt ctaaaataac
     9901 ataattaagg ttttttaatt gaaaactgaa catttatggt catcctttaa tgaaagcgct
     9961 aactacataa gttagaatga gtttgactga tatattagtt tgcttatcag aaaaacaaaa
    10021 tggacggagg aataacatga gtgatattat tattggtatt ttgttagtta tctcattctt
    10081 tttcatggtc tggtactgtg taaaaggcta caacttgatg gtcggctttt gtcatcatgg
    10141 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac tcggcgatgg
    10201 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
    10261 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
    10321 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
    10381 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
    10441 gaccagtgat ttctattgca gttattgtgt tgccgatctt gatgtcatta ggtatttcag
    10501 tcaagtccac aatcttgtgt aaaagttcaa cctcatcaaa agcacaatgt tttgcgttgt
    10561 gaacctaact gccatgcctt tctttctgag gagagaggtc aggagcagta gcgcagcgac
    10621 tcttgatgtc tctcctcaga aagaagagac tcgcacaggt tcatggtgct aaatgttatt
    10681 cgaattaaat tctaaatatt ttcgtggaga agccagccac tcagtgtctt tttccatcaa
    10741 aagtgttcca attatccgag tgatggaatt gatatttgga aaaatccgaa tcactcgttc
    10801 tctccggcga atctcttcgt tcaaccgttc caacacattg gttgtacgaa tccttcggcg
    10861 gatattttca ggaaaagaca aaatttgaat agcatcttca aagccgtttt ctaaaatgtc
    10921 gcaagcagca cttaattttg aatcacaacc atatttttcc aagaagtgct cttttctttc
    10981 acgtgtcaat tcaagttcag aggctttaaa gatactcttc aactcgtctt ttacatcaga
    11041 agaaactttc tttggcagct tatcaaaaat atttctcaaa aagtgggctt ggcatctttg
    11101 ccagctcgca cctaaaaagt tcttgcgaac agccttaact aaccctttgt gtgcatcaga
    11161 aatgaccagt tttacaccga acaaacctcg ttgaacgaac gattgaaaaa cggtatccca
    11221 attatcttcc gattcgccgt cttgaacatc aaaaccaaga atcgtacgtc gtccatcaga
    11281 atcaatgccg ataccaatat atacaccttt tgagacaata cgatgatttt cccttacttt
    11341 catatacagg gcatcacaca tgaaaaatgg atattccgag ccttctaggc tccggtttct
    11401 ccactcaaaa acaaatggat ccaactgttt catcagattt gaaacgaatg atttagaaac
    11461 agtcgcacca tccgttagca gttcaactgt tttagttact ttgcgagtgg agacaccaga
    11521 aatgaccatt tcaatcatgg cagcgatcaa agctttttcg cttcgctgat atctttcaaa
    11581 gatagtagga ctaaacttac cgtctctggt tcgtggaaca tgaagttcta acgtaccaat
    11641 tcgagttgta aacgaacgat cgtagtaacc atttctttgt gattgccgtt cttctgaacg
    11701 ttcataggca tttgcctgaa tatattgatt tcgttgttct tccatcagct gattaaacat
    11761 gatagtcata agtgtttttg cggtatcatt cgcaccgctt tcgtttataa ttgcttgaat
    11821 ttcttcgctt tcaaaagtaa aatgtacttg agtcattgat atcagtccta ttcgagtatg
    11881 ttttctgcaa aaaacattgt actcctaact gagatcaatg gcttttttga tgcttcactt
    11941 ttacacaatt atatggacgt aatcggtatt tcagtaccgg ttgcactgtt ctcatttatg
    12001 ggctcgatta tggctggaat ctttgctaat atcgtcaatt tcaaacaata tcaaacgatt
    12061 tatgctggtt tcaatccagc ggctgaaagc tatacttata atgactattt ccaaattggt
    12121 atgattggga tgattgtaag cttagttgtg gtactaacgg tcgctaatat atcaatgaac
    12181 aaaaagaaac gctatgcaat ggcagcaaac gtaccagctg aaggtggcga cgcaccaatg
    12241 atctcatggt tagccgtatt attaccagtt ttaggggtcg ttcttttaga tcttcaaatc
    12301 atcttaggtt ttattctagc aggtatctgg gcattgctgt ttactggaaa attgcgcgga
    12361 ggttataaag aaatctgtcg tcagtttgcg aaattattta cagatggcgc agtcgatgtt
    12421 gcaccaatgg ttggtttctt gatgacctta gcgatgttca ataattcagc tgcgtatgct
    12481 tctccttatt tttcagctat ttttggagat tggataccgc aatcaccatt agtcttggca
    12541 atcgtatttg ctatattgac accactagga tttttccgcg gtccaatgaa tctagtcggt
    12601 tcaggctcag ctattttagc tgtcgtgtta gctgttaatc caacgatgtc gccagccttt
    12661 ttgttcccgt tatttgcaat tacaacgatt gctcctcagc atttagatat tacacaatct
    12721 tgggttgctt ggggattagg ttatacaaaa gtaacttctc gagaatatat gaagaaatcc
    12781 atcccaactg gttggatcat cggtgcgatc ttatgcctga tcacgttctt attgtacggg
    12841 aacgcctagt aaacaaaacg tcgataaaaa cgattagata aatggaggaa aagaaaatga
    12901 aacgttttgt gcgaatggga attgatgttg gcggtacaca tacgaaagca gtcgcaatcg
    12961 ataatgcgac gcacgaaatc atcggaaaat cttcagtgaa aacaacccac gatgatgtcc
    13021 gtggagtagc tgcaggtgtc gttcagtctt ttcaaaattg tttgcgggaa aataatatca
    13081 gtccagaaga tgttgtgttt gttgcacatt ctaccacaca agcgactaat gcactgattg
    13141 aaggagacgt agcaaaagtc ggcgtaatcg ggatggcaaa aggtggatta gaaggttttt
    13201 tagcaaaacg acaaactaga ttgaacgata tcgatttagg gaataagaaa aaaatagaga
    13261 ttgtcaatgc attcttacct gtcaaaaact taaatgtaga ccgtgtttca gaaacgattt
    13321 cttctttaga aagagaaaga gcggaggtct tggtttcttc tatggcattt ggagtagata
    13381 atggggaacc agaacgagtt gtttatgaag cagcatctgt gaaagcgatc cctacaacga
    13441 tggcttctga catcacaaaa ttatatggat tgacaagaag aactcggaca gcagcaatca
    13501 acgcatctat tttaccaaaa atgctagata cggcaacctc gacagaagac tccgtacgag
    13561 aagcaggggt caatgtttct ttgatgatta tgcgcggaga cggcggagta atggaaatca
    13621 atgaaatgaa aaaacgacct gttttaacca tgctttccgg tcctgctgca tcagtcatgg
    13681 gctctttgat gtacttgaga gcgtctaatg gtgtttattt tgaagtaggc ggaacaacaa
    13741 ccaatatcgg ggtcattaaa aatggacgtc cagcaatcga ttattcaatt gtaggtggcc
    13801 acccaacata tatctcttca ttagatgttc gggttctagg tgtggcaggc ggttcgatgg
    13861 tacgggctaa tcagtcagga atcatcgatg tcggtccacg ttctgcacat attgccggtc
    13921 tagattatgc agtatttacg gagacagaaa aaatcaaagg tcctaaggtt gaatttttct
    13981 ctcctaaaga aggagaccct gctgattacg tcaaggtagt gatggaagac ggagaagaag
    14041 tgactattac aaatacatgt gcagccaatg ttcttggatt agtgcaagaa gaacattttt
    14101 cttatggaaa tgtcccatct gcacgaaaag ccatacaggc attagcagac tattgtcata
    14161 cgacggttga agacatcgca gagcagatca tggaaaaatc ctatgctaag attgaatcgg
    14221 tgattttaga attggcagac aagtatcact tggagaaaga tcagatttct cttgtaggtg
    14281 tagggggcgg cgctgcctca ttgattactt actttagtaa taaaatgggt gtgaaatatt
    14341 cgattcctga aaacgccgaa gtgatttctt ctattggtgt agcacttgca atggtccgag
    14401 atgttgtaga acgtatcatc ccttcgccaa gtaaagaaga tatacgatcg ttgaaaaatg
    14461 aagcgatgaa taaagcgatt gaatcaggcg ctacaccaga atcgatcgaa gtccacgtag
    14521 aaatcgatcc acatacttca aaagtaacgg ctatcgctac tggttctacg gaagtgaaag
    14581 caaccgatct aaccaaggag atcacaacag aagaagcact agagcttgct gcagaggata
    14641 tgcgattaaa taaaaatgaa gtttgcttat tggaaaatac acctttcttt tatgtatgcg
    14701 gcgaacagaa tcgttcaaaa aatgctggaa gtcttcggat catcgatcaa aaaggattta
    14761 tcaaggtcca acgtggacat gcttcatgta tgaaaacaac tgcagccaat tatatgacag
    14821 ctgtcgaaca actttgggaa gatatggcag tttatcaaac tgaattgatt gcccgaccag
    14881 aattttatct ctgcttagga gcaagagtct ctgattttac agcgacggat ctggaacaac
    14941 tacagctttt gatggatctg gaagtatcta ctatggaacc ggaggaagaa gtgatcgtcg
    15001 ttgccggaaa tatcaaacaa acataagtta gaagagttac taaacgaatt acaacagatc
    15061 cccgaagaaa tttggggatt ctaccagttt cggcgagatt tattttggaa aaagattcct
    15121 ttgtccaagc agaaaattct tatacaacaa tcaatcgatt gtggtatcga aacggcatgt
    15181 agcatcaaaa aaaagtaccc ttttgccgat gtaggagaaa tctgtgaaca aatggctgtt
    15241 ccaatcgttt cctgtgaatc agagcaaata aacgaacgaa tcacttttgc aacttatgca
    15301 gaagatgaag ggatccgatt gatgacggag ccgttagaaa aattaaaatg ttcaggacta
    15361 gcttcgatct ctaaagaaac agcgcaagct ttgattattg gacatgaact atttcaccac
    15421 atcgaagctt ctgttaaggg aatttatacg caaagcgaaa aaattgtgct gtggcggttg
    15481 cctttttata cacatcaatc aaacattcgt gcattgagcg agatagcagc gatgagtttt
    15541 tcaaaagaga tgaatcaaag cgatttttcg ccttatgtat tagaggcagt cttactttgg
    15601 ccatataatg aaattcgtag ccaagggatc ttagaagaaa taaaagaaat agaaaaaaga
    15661 tgtacggagt atgatttaga ttacaaataa agagactgtg acaaaagtcg tgtcacagtc
    15721 ctttttttcc gaataaacgg tgttctcctg tcaactcctc ggtcacaagt caaaatattc
    15781 aacaaacatg agaagctgtt ttttgaaatt gttccttgtg actcggagat gaacggcagg
    15841 ctcacgacct cttggaaaaa ggtgttcaaa agcttgcatc tgcggtaata agaacagcca
    15901 agttaaaaac ataagaagct atttttactt ggctgttctt attacttgat gcagggcagc
    15961 tttttcacaa cctctcaata aacggtgtgc aatcagctga ccctcggtat tagctcaaaa
    16021 aaaacggcaa aatatgagga actattttgc cgtttatttc tctaatactc aggtcaaaac
    16081 gctgattcca aaacctctta tttgatgggt ctgtcctgaa tatcttcaaa ataatcgggg
    16141 taattatgaa tcacgtattc ttgctcctcg ataatcatat gtaaatgcat cgtgcaaaga
    16201 aatccgactt catcaaagtt atgatcaatc attgattgaa gcattgtctg atgttcatct
    16261 aatactcgac cccagtctaa ttctgaaatc gtcaaacgta accatctgaa acggttcaag
    16321 tgcgtactat aactttctag ccaatcccaa atttctcgtt ttccagcaat ttcataacaa
    16381 gttcggtgaa aatcagtatc caaatcaaaa aaagcttttt tattttgagc gatcactgcc
    16441 tgatcggttt gttctagtat tttttctagt ttagcttgat cttgcttggt cattttcgca
    16501 ctgcattctt gcaggatcgg tttttccaac ttttcccgcg tataacgggc taatgaagca
    16561 gatcggatat cgatttttga aacataagtg ccggactgtg gaatggtttt tacaagctct
    16621 tgattgcgca gttggatcag tgcttctcga atcggtgtac gcccgatagc tagttgttct
    16681 tctaacactt tatcggagat tttacttccg ggtgctaact cggaataaat gatttgttta
    16741 cgtatagatt cataagcttg ctgttgcaaa ttattgataa ccatttaacc ttcctttcta
    16801 tgcagatata atctattata acataaatcg cttacaaatc gtgtgggcat aaaaacacag
    16861 tctgcaaaaa aagccctgat aggcatgcct attagagcct cgaaaagaaa aatattgaat
    16921 caatctggtc gtacctcttg gatcggtttg acacgaccgc cgattctttt agcaaaagct
    16981 tcggattctt ttttgtcttt gaaaatgagc agattgtcta agtttttttc ccgagtgcga
    17041 cagccagttt tatcgatgta accgtgcatt actttaacga catacatggg gatcacctgc
    17101 ttttttcttt tcattatacg aactactcga cacaatacaa gaaaaatgct tagacggtct
    17161 ttattaagaa tttacatcat ccaaagaagc gtacgcttgg aataactgtt ctaactccgg
    17221 attgactttg ctcagcttca ctaatcgttg cgaatcagaa agtaaaaaac agtgtttact
    17281 actgccagta gtttttaatt ttttgtcttc agttcccaca atttcgtagc taaaatccaa
    17341 ccgtgttccc gtatactttt ggatagttgg gtggatgctc actttttctc cgtaatggat
    17401 catttcttta tagctgcaat ttacttccag aacggggatg atgatcccag attcttcgat
    17461 ttttttatag ggaaacccta gatgtaccaa taaggctacc cgagcctctt caaaccagcg
    17521 gatataattg ctgtgatgaa taatccccat ttgatctgtt tcataataaa aaggttctcg
    17581 cacataacct gtaaatttct ccatgactca tgcctcgttt tccattgatt ttttacaaaa
    17641 ataattcatc cacttcggaa gtgcttcgct gatttgtgtc ggcaaaacaa tatattgttc
    17701 ttttgccagc tggtctccaa tgaaactatg aagataaaca gctgctgcaa tcgtttggtc
    17761 attttgagga aactgggcta aaaatcctgt gatcatccca gctaaagtgt cacccatgcc
    17821 gcctgttgcc atagcagcag aacctaaagg attggtgtag aagtgctggt gaccataaat
    17881 ttttgtctca tgacttttca aaacgagcac agcaccgatt tcctgttgct tctttttgtt
    17941 ttctttatcc cgttgttctt gtatcggtag agaactcaac cgttgccact ccatttgatg
    18001 aggtgtaagt acagtttgtt cgggataggg tagcaaaaga tgattagcaa caagtaaact
    18061 taaagcagag ccatcaatca ccagccattg ttcttttgat tggttttcta gtacttgttt
    18121 gaaacgttcc aaactcttag aagaagtacc caatccggga ccaatcaaga gtacatctgc
    18181 ttgctttatt acggaaagaa actggtcagt atccgtccaa tctacaatca ttgcttctgg
    18241 cagatgactg tgaagagcac tccgattttt ttcatcagtt actacagtcg tgagacccgc
    18301 tccactgtgt acggcagcta aagcactcat aatgattgct ccgccatatt gttcattgcc
    18361 gccaatcaaa acgatacgtc caaatgttcc tttatgtgaa atctttggac gaggtgtgat
    18421 gactcttttc actagttgtt cgtttatcag catcgaaaaa cctcctttta ctcattatag
    18481 cgtatatttt ttttagagaa aaacagataa gaaaaggaat tgtcagattt tttgaactat
    18541 ttttttctgt aatttgtggt atcatgaaca cgaagactta aataagtgga ggattaactt
    18601 tatgacaatt gattggcaaa aagaagtaga agcacgtaaa gaagacttgc ttgaagactt
    18661 gaaaaatctt ttacgtgtca acagcgagcg cgatgattca aaagtaacac cagatgcacc
    18721 atttggacct ggacctcgcg acgcattgaa acatatgtta gcttatggcg aacgtgatgg
    18781 atttaaagta aaaaatgtcg ataactatgc agggcatatt gatcttggcg aaggagacga
    18841 aacgctaggg atatttggac acatggatgt cgttcctgca ggagatggct gggacacaga
    18901 tccttatgaa ccagtaatca aggatggaaa gatctttgca cgcggatcaa gcgatgacaa
    18961 aggtccaagt atggcagctt actatgcgat gaagatcatt aaagaacttg acttgaaact
    19021 atcaaaaaaa gttcgcttcg tagtcggcag cgatgaagaa agcggctggg gcgatatggc
    19081 ttattatttc gaacatgaag aagaaccaga ttttggtttc tcaccagatg ctgaattccc
    19141 aatcatcaat ggtgaaaaag gaaatgtttc tttagcttta cgcttcaaag gcgacaatgc
    19201 aggagattat gtcttgaaat cttttgtttc cggacttcgc gaaaatatgg tgccaggaac
    19261 agcaactgca gcacttcaag taccaagtgc agatgcagct atcgcgatgg aagaagcatt
    19321 ttaccaattc atcgaagcaa atccagtaag cggaacgatt gaagcggaca atacgtatgt
    19381 aaaaatcgaa ttagttggta aaggcgcaca tggtgcaagc ccacaatctg gtatcaatgc
    19441 aggttctttc ttggcattgt tcttagataa ctatgaattc ctaggatctg ctaaacaatt
    19501 tatccatgta gcagctgctt atgtccatga agatttttat ggagaaaaac taggagtcgc
    19561 ttatgaagat gaaaaaatgg gcaaattgac aatgaatgct ggactatttg cttttgaaga
    19621 aaatggtaca gaggaagcga acttcatcaa tatgaatttc cgttttccaa aaggtgtaac
    19681 agtagacgga ttgcaaagtg acatcgaaca aacagtgggg caagaaggcg caacagttac
    19741 tcgaggtgct cgcgtaatgg aaccacatta cgtaccaatg gatgatccat tggtggctac
    19801 attattgcaa gtctatgaag accacactgg agaaaaaggc tacgaacaaa tcatcggtgg
    19861 cggaacttac ggacgtttgt tgaaacgtgg cgttgcttat ggagcgatgt tcccaggata
    19921 tactgataca atgcatcaag caaacgaatt tatgagtctt gatgacttgt tcagagcaac
    19981 agcaatctat gcagatgcca tctatcgttt ggcgaaataa aaatactaaa aaa
//
LOCUS       DAGCYL010000034        20031 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.035, whole genome shotgun sequence.
ACCESSION   DAGCYL010000034 DAGCYL010000000
VERSION     DAGCYL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.035"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            86..382
                     /locus_tag="MB216_000955"
     CDS             86..382
                     /locus_tag="MB216_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBT4525809.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(1026..1979)
                     /locus_tag="MB216_000956"
     CDS             complement(1026..1979)
                     /locus_tag="MB216_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4525810.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(2002..2757)
                     /locus_tag="MB216_000957"
     CDS             complement(2002..2757)
                     /locus_tag="MB216_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4525811.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2754..3713)
                     /locus_tag="MB216_000958"
     CDS             complement(2754..3713)
                     /locus_tag="MB216_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBT4525812.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3706..4656)
                     /locus_tag="MB216_000959"
     CDS             complement(3706..4656)
                     /locus_tag="MB216_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBT4525813.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4806..5258
                     /locus_tag="MB216_000960"
     CDS             4806..5258
                     /locus_tag="MB216_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBT4525814.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5347..7614)
                     /gene="gshAB"
                     /locus_tag="MB216_000961"
     CDS             complement(5347..7614)
                     /gene="gshAB"
                     /locus_tag="MB216_000961"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBT4525815.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7801..8799)
                     /gene="ftsY"
                     /locus_tag="MB216_000962"
     CDS             complement(7801..8799)
                     /gene="ftsY"
                     /locus_tag="MB216_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBT4525816.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8810..9619)
                     /locus_tag="MB216_000963"
     CDS             complement(8810..9619)
                     /locus_tag="MB216_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4525817.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9628..13209)
                     /gene="smc"
                     /locus_tag="MB216_000964"
     CDS             complement(9628..13209)
                     /gene="smc"
                     /locus_tag="MB216_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBT4525818.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13229..13915)
                     /locus_tag="MB216_000965"
     CDS             complement(13229..13915)
                     /locus_tag="MB216_000965"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBT4525819.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14263..16044)
                     /locus_tag="MB216_000966"
     CDS             complement(14263..16044)
                     /locus_tag="MB216_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4525820.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(16070..16984)
                     /locus_tag="MB216_000967"
     CDS             complement(16070..16984)
                     /locus_tag="MB216_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4525821.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(17011..17973)
                     /locus_tag="MB216_000968"
     CDS             complement(17011..17973)
                     /locus_tag="MB216_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4525822.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17973..18917)
                     /locus_tag="MB216_000969"
     CDS             complement(17973..18917)
                     /locus_tag="MB216_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4525823.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18918..19922)
                     /locus_tag="MB216_000970"
     CDS             complement(18918..19922)
                     /locus_tag="MB216_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4525824.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5576 a         4288 c         3558 g         6609 t
ORIGIN      
        1 aagaattact ttttgttcac ggattattaa caactcttcg gtagagtaaa cttatcaaat
       61 aaaagacttc tttggaggaa tcactatgaa ggaatatata aaaaacatct attttatcga
      121 agaaacacaa aatatcgagg gttcttatat tgaagtaaaa acactctttg tcaatgaaga
      181 taaaaccaaa gcattggata tttataaaaa attagcttca aaaaaaacga atagttttgg
      241 attgatatta agcgagtata aaatcaaagc agaagagagt tatttctatc aattattaaa
      301 acggtggagt aagcttcccg ccgattttta tcgaaaaatg cagatcatca actatcaacc
      361 gcttgctgag acacatgcct aaatagaggt tgtgaaatca gtgctttgag ctaataccga
      421 aggtcagctg attgaacatt gtttagaaga gattgtgaca actgcgattt gctttgagta
      481 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtggg cttattgccg
      541 agaagcagca gatggaacat cgtttagaag agattgtgac aactgcgatt tgctttgagt
      601 aataagaacg ctggacagaa aatagcttct catattttct agtctagtgg acttattgcc
      661 gagaagcagc agatggaaca tcgtttaaag aagagatcgg tacaaaataa aaatatctaa
      721 atttaagttt tatctaagtt tcgctttgta cctttgattc aaatcaaagg aggcgatctt
      781 gatgctgaaa aaatggatca cactattttt tgccactccc cattctagaa tcaacccgta
      841 tgatgtagat aatgaattca cgttgaaaaa agatttctca aataaataaa aaccatccgt
      901 aaaatcatca atacgatttt acggatggtt tttgataact tctttgagga taggctcttg
      961 ctatttaaga atgtaagaaa cattattgtt acatagtatg agagtttaat cctctgttat
     1021 ttatgctatt taaatgcttg attcacatct tcgatcatca gtttcattga ttctaaaccg
     1081 cccccgctta aataccagac atctggttca agggagatga tctgctgatt tttaccagca
     1141 ttcgtttgtg ctaccagttc attggcagaa atatcatctt tgctgtcgtc tccaccaata
     1201 gcttttgttc tgtctactac aaataagata tccggatttt tttccagcac atattcataa
     1261 gaaacacttt gaccgtgagt ggatgcttcg atttgatcat ctgcttgttc aaagccaaat
     1321 aaatcatgga tgaaaccgaa tctagaacca gatccgtagg ctgataattg cccttcattc
     1381 acaagtgtga ctagtgcttt tttgtcagta gcttctgctt tctttttcgt ctgatcgatt
     1441 gcactagata attcctctaa ttgagtttta gcttcttctt gcttaccaaa gatctcccct
     1501 aaagcagtga cattttgctg gaaagattcc catggatttt tcgcatctaa agaaaggaaa
     1561 atggtcggag caatttgact tagatctttt tgaaaatctc tttgtcggcc agaaatgatg
     1621 attagatctg gctgaagctg attaattttt tctaagtctg gttcttttat tccacctgct
     1681 gattctactt ttttatagga tgaaagatac tcaggaaggc tgcttgtagc tgctccaact
     1741 accttatctc caacaccaag agcatccatc gtatctaaag aaccattgtc gaatacgacg
     1801 actttttctg gattatttgg cacctcgatt tggtctccat tactatctgt tatcgtcaat
     1861 gtagtttcag tttgtgtgct gctttttttt gtgtctgcct gactggtgtt gttgctattt
     1921 ccacatgctg ctaaaccaaa tgatgctaga atcaagatac ctactactaa ttttttcatc
     1981 tcttctctcc taatgttagt tttagaaata aagacagaat ctgcggcctt caatctcaca
     2041 aatccgtatg tccatttcgt ataattcatc caatacatct ttttttatca tttcttgggt
     2101 cgttccttgc gtaaataact gtccatcttt cattgcaaca atttcgtctg cataacttgc
     2161 agcaaagttg atatcatgaa gaacaataac aattgttttc ccgatttctt gtactaaatt
     2221 ttttaaaatc tgcatgattt gaaccgcaaa attcatatct agattgttca aaggttcatc
     2281 cagtaaaata taatccgtgt cttgcgctag caccattgca atatacgcac gttggagttg
     2341 accgcctgaa agagtgtcaa tcgattgatc cgctatttct tctaacccaa ggtgttccag
     2401 tgattcctga acgatttcct cgtcttttgt cgtcaatttt cctttcgtat aaggaaatcg
     2461 tccaaattct actaattctt tcacggtgat ttggacattt atttcattcg attgtcgtag
     2521 gatcgataat tttttggcta attcattgga tttccaagcc tttacttcgt tatggtctaa
     2581 atagatttca cctgtatctt ttggtatcaa acgactgatc atcgacaaca ccgttgactt
     2641 tccagcacca tttggaccga taaaagcagt caatttcttt tcaggaatcg ttaactgtac
     2701 atctttaacc actgattttc catggtacag tttattgacc ttttgtaaat caatcatagg
     2761 atactcttcc tttcgcgaat caattttgtg acaaaataaa ttcctccgac aaattcaacg
     2821 acgacactca ctgttgtatt ccagccgaaa atctgctcta ctgcgagctg tcctcctgtc
     2881 agaaacagca tagctaccag aaagcttccg ataaatagct gaccatgacg ataagtagaa
     2941 aagacacgat aactgatatt tgcgacaata aaccctaaaa aagtaactgg acccacaaga
     3001 gccgttgatg tccctgtcaa aagactgaca caacataaaa tcaaaaattc aaaacgatct
     3061 gtgtctatcc cgaggtttac tgcttgttca tttccgagat ggaaaacgtc tagcacatgt
     3121 cgatatcgaa ataacaggta ccagcagccg ataatgatca aagaagcagg caaaagatac
     3181 tgactctgga tattgccaaa gcttgcaaat aatctgcctt gaagcaaatc atattcattc
     3241 ggatccatga tgacttgtaa aaaggtactg acactaccaa aaagcgtacc taaaatcatc
     3301 cctgacatca gtaataaaaa aagattcgct ctcatttttt tcaaaagaaa gaaaaataac
     3361 aatagactca gcccgaccat cagcccaacg ttcaataaga aaaaaactgt acctgtctgc
     3421 tgaagcatcg tcactcctcc gataaagaag aaaaggaccg tttggacaag gacatataac
     3481 gactcaagtc ccaatactga aggagtcaaa aactggttat gcgtaattgt ctgaaagctg
     3541 attgtggcac tgctgacagc tagcgagaca aggataaatg caaaaatttt cttactcctc
     3601 aatgcacatg caaaatgcca gtttccatat gtatggatcg tcaagtacat tcctgtaaca
     3661 aataaaacaa gaacaagcat accaataaaa atagatggct tttttttaag catatcgtcg
     3721 tccccttatc agaagataaa ggaagatagc cccaccgatg attccaacga tcacactgac
     3781 tggtatttca taaggaaaaa tcaacacgcg agcgacaaga tcgcaaaata ctaagaacaa
     3841 actacctgct aaagcagtta cgccaattgt tttgtgcact tgatctccat atattctaga
     3901 gacaagattc ggaatgatca ctccgataaa aggaatgctt cctaccatga tcaacatgac
     3961 tgcactagcc aatgatacta agccaatgcc taaaaactgc atcttctggt aatctattcc
     4021 cagatttact gcaaacgatt cgcccagacc aatgatcgta aatcggtaag caaatagcca
     4081 aaccaagagc aacaatggaa caacagcata caataactca taatttcctc gtatcacagt
     4141 cgcaaaattc ccttgaagcc atgaagtcat attttgtacc aactggaatt gataagcaaa
     4201 aaaagtagca actgaaccaa tgatattccc taacatgact ccaatcaacg gaagcatcaa
     4261 ttgatttttc tggaggaaca atcgcgtaag ggataaaaaa agcaatgttc ctgcaaaagc
     4321 aaagcaaaat gctgtcaagg atcggacaat caaggatgca ccaggtagga aaatcatagc
     4381 cactaaaata cctaaacgcg cactatccat cgtgcccgca gtcgttggtg aaacaaattt
     4441 attttgggtc aaatgttgca tgatcaaccc acaaatacta ctggtcgctc ccacaaggat
     4501 caaacaaatc gttcgtggca aacgagtcac tagcaagatt tgtaagcttt gcccatctaa
     4561 ctggagcagt tctgacaatt gtacattcga tactccgatg aacaatgaaa tactacttaa
     4621 aataactaat aatgctacta gtcgtccgta gcgcatccaa tccacctttc taattgagaa
     4681 tgattttcat tcttatttta agtgaatcgt tgaacaaaag caaccgcttt ttcatatgtt
     4741 ttaggtatta ttgggaatat gctataatcg taagaaagac aaagagaaga aggagggtat
     4801 tttttatggc acaagatgat gtaaaaagcc atttggataa gggactttat ggtacacctt
     4861 tagtgaaccc tgaagaacag cataaatatt tagggacatt cagggaacgc tgctatctta
     4921 gtatgactgt tgctgaaatg atccaaccaa aaaacaaaga aaacttttta aaagaactca
     4981 aaaaacatcc agatgcttcc atactcttga atggggctct acccctttct gtccaaaacc
     5041 aatatatcca gctagcaacg aaaaacaata gccgtttcac tgtagtcaac gattacgtca
     5101 ctgcaaatcc tgaggaattc ggattgctct taacagcaaa agaagcagta aatgaacctg
     5161 tgattgatat cgaaaaaaaa tacccagaag aaaactcttc gccagctgaa aaagaaaaac
     5221 caaaaaagaa aaaaagcttc tgggatcgtt tattttaaaa cgaaaaaaca cataaatttt
     5281 tacatgcaaa aaaagctttt gatcagattg acatcaaata ggacaatctg tcaaaagctt
     5341 tttcttttaa aatacttcag gatataacaa tttcaataca ttcatcgtca atcttctgcc
     5401 ttttcctgca aaaggataaa catgcatatg catggctgga ttgaaatttg cttcgatgat
     5461 cccatatgct tttttatcag ttgtaggatc gatttcttta tctggtataa tcaaatcgat
     5521 accactaatc ttagctccta gtgcttctac cgcagaaacg gcgatttttt tgtaagcttc
     5581 agaaaattca tcagtcatat cgatcgaatc accacctgta ctgatatttg agttctccct
     5641 tagataaaca atttgattgg cttggggaac tgattcgatc gtcaaccctt gttctttcaa
     5701 catcaattgt tctaattcac ctaattggat caattctaaa ggtgcacgat gattcgttcc
     5761 acgcaaaggg tcactatttt tttcttcaac aagttctttc actgttcgga tactgtcacc
     5821 aataacatta gcagggactc gcaacatgat ggcttggact tctccgtcga tcacaaagaa
     5881 acgatattcc gttcccggca taaattcttc tactaaaacc gaactgtctt cacgaaaagc
     5941 aatggctaaa cctgcttgat aatcttccag actagctcct tccttgaaaa tcgtgatacc
     6001 taacccgtaa ttggttgatt taggtttgat cacaaatgcc tgctcggcaa atctcggata
     6061 agcttttacc gcttcttcca tggatgaaaa ttctgcgcct cctggtaccc ggaacccagc
     6121 ttcttttaag acttttttcg ttactgtttt gttttccatg ataagtggga cgatatagct
     6181 gtccttactt gtcatgtttg catttttcac atattctaca tggtcttgga attgcagacg
     6241 caaaaattga tccgactcat ctaatacttt gacttggaca cctttttgga tggcatcgaa
     6301 cataaatatc tgagtagaaa gttccatttc gcgaaaaccg gccaactgat agggacgttc
     6361 atggctttct ttataatatt gatgtcccaa tgccacagca acttcttgct gactcgatag
     6421 ctgtgcttct tgatagagtt tcgctgctaa cgtttgttca ggatgatcca tttgagtcaa
     6481 tgcttcctct actaaataaa gagaatccaa ccaatccaat tcaaccagca tttgcttcat
     6541 tccttcaaga atttctctac cttcttgatg aaattctgtt tgatctgatg gttgttctag
     6601 tgctactttg ttatttcgag tcgtaccttc tgctacccat tggtcacagt cttctttttc
     6661 ttctgtccat aacatgtaca tcaggaataa ttgtaagaac cgaacttgct ctggattgat
     6721 acccaatctt gcataaggat tcaaatcgat attccgcaat tcaatataac gaacacctgt
     6781 tgttgctaaa tctgcgactt tctttccgcc tcggaaacga agcggtgtat aaaattcttt
     6841 ctcctccgaa agtttccctt cttcgatcat tcgctcgata tctgctaaat actgtttcat
     6901 agaagcatag gaaactttca catttggatg attcgtatag cccagcgcac tgttgcgaat
     6961 actgcgaaca ggttcttgcg gatgtgattc atctaaaaag tagttcttct cactcattgg
     7021 tgaagcaccg aataaataag tgatcatcca gcgatagcgc ataaagtttc tcgctgtttt
     7081 caaataaagt tctgttttga attcggaaaa atcttgaaat tcttcctgat ggctgaacaa
     7141 tgttcttagc aactcatcgc caaattcaaa attgaaatga ataccgctta ccatctgttt
     7201 tcgcttcccg tattcttttg ctaaataacg tcgatacaag acatcttcga aattttttaa
     7261 ttttgcaata atgatctctt cgtctttttc cggtaaggca ggtggcatac ttaatggcca
     7321 gatcatctct tctttcggta tagaacgagc agtcacatca taaacagcag cgagatactg
     7381 aaataattcg ggaatagaat cggtaacagg ggtgatcatc tctgtttgtg tttcgctaaa
     7441 atctgtttgg atataggggt gataggatcg atcgccaaag attgctggat gatcggtttt
     7501 tgctagattt cctgcaagat caacccgctg tccttcccgt tctataccaa atcgagcttg
     7561 gtcgataaaa gggcgcgcat tgacatgcaa taataattgc ttaaaattca tcattaagtg
     7621 acctccaatt catatgaatc taattataat caaaaagtct aagaaaaaga acgaaattgt
     7681 ttgagaaccg cttacaaata agtataaaaa tgaaggcatc aaaaacacag ctatgttaaa
     7741 aacaatcatt caaacaagga gctgcaactt ttgtcacagc tcctgctttt tagacatggg
     7801 ttattcttct tttagtaatc ctttgaacaa tccaacgaca aaatcatttg gatcaaacgg
     7861 ctccaagtca tcgattcctt caccaagacc gaccaatttc actggtaaat gcaattcatt
     7921 acggatagca agtacgatac cgccctttgc tgtaccatct aattttgtca aaacaagacc
     7981 ggtcacatct gttgtttctt tgaattgttt tgcttgaacc atcgcgtttt gaccagtggt
     8041 tgcatctaat acaagaagtg tttcttgggg tgcttctggt aattcccgct ggattactcg
     8101 ttttattttt tctaattcgt tcatcaagtt cactttattt tgcagtcggc ctgcagtatc
     8161 aactagtaaa acatctgcct gctctgtctt tgctctttcc aaagcatcga atacgacagc
     8221 tgctggatcg cctcctgcat tgccacgaac aacttcaaca ccagcgcgtt cgccccagac
     8281 aactaactgg tcgattgctc cagcgcggaa ggtatctgct gctgccatca atactttttt
     8341 accttcgttt ttgaactgat gggcaagttt accaatactt gttgttttcc ctacaccatt
     8401 aacaccgaca aacaaaataa ctgttagtcc gtcttcttgg atatttagtt catttacttc
     8461 attgattcct tccgcttcat agagatccac aagtttctcg atgatcgcat tttggacttc
     8521 tgccgatttt ttcgcatttc tgagtttcac ttcttgacgt aatgcttcgg taattcgtat
     8581 cgctgtttca aaacctacat ctgcaccgat aagtgtttct tctaattctt caaaaaagtc
     8641 ttcatcgact gttcgaaaat tggcaaacaa ctcattcatc cgttcaccaa atgttttacg
     8701 cgtcttttct aaacccttgt cgtacttctc ctgtacttca acttcttctg ttttctcttc
     8761 gccagtaaaa gcacgcttga ttttatcaaa aaatcccatt tgactccccc tactctaata
     8821 caaatttttg gatcactttg gctacgccat tttcttcatt gctggctgtc acgaaatctg
     8881 ctgcttcttt gacgaaaggc acagcattct ccatcgctac gcctagtcca gcatattgga
     8941 tcatcggtaa atcattttcc tcatcaccga tcgccatgat ttcttcttgt ttcaaaccaa
     9001 gatcttttgc caataaagaa ataccataag cttttgtaac accttttggc ataaattcca
     9061 aaagcatact tcttgttttg atgatctcat acatttcatg gaattctgct ggtatttctt
     9121 tgattttagg atctaattct tcttggtcat aagcgattac tgccttatta agcacaaatt
     9181 catctgtaat atcgcttata tccgctggtt ggaattgcag cagcttattc aacacattgt
     9241 acaaggattg cttccttggg gcactaggca gctgtaaaac cacattgtca gacaaaacat
     9301 ctagtggcag atccaattgc tgagccagtt tgtataagcg ttggatatct gatacttcca
     9361 tcactttctt ttccatgatt tcaccagtat catttttttg taccaaaccg ccgttgaaag
     9421 tgatgctgta gtcaccttca tctaccagtc ctaattcttg cagataatgc gccatcgcag
     9481 ctaaggggcg tcctgtacat agaacgatct tgaccccttg ctgttttgct tgtgctaatg
     9541 cttgtttatt ctcatctgaa atcagctttt gttcatttaa taatgtaccg tccaaatcga
     9601 ttgctactaa tttgatcatt ttcgttccta cactttcgtt tcgttgattt tcgcctcttc
     9661 attgatatct tccatccgta cagaaacgat ttttgataca ccggattcct gcatcgtcac
     9721 tccgtacagt acatctgctg cgaccatcgt tcctttgcgg tgtgtgacta cgataaattg
     9781 tgtgtcatct tggaaatcgc tcaagtaacg accaaaacgt ttcacgttgg cttcatctaa
     9841 cgctgcttct acctcatcta agatacaaaa cggaacagga cagacacgga taatggaaaa
     9901 gagcaacgcg atggctgtaa gtgctcgttc tcctccagaa agcaaactta gactttgcag
     9961 ttttttgcca ggaggttgta cttcaatctc aatacctgtt ttcagcaaat cagttggttc
    10021 cgtcagtact aattcagctt taccgccgcc aaacatattt ggaaagacaa ttttgaattc
    10081 ttgacggata gcttcaaata cttctttgaa tcttgtccgc acttcatcat ccatctcatc
    10141 catcgtttca aacaattggt ttttcgctgc taacagatca tctcgctgtg ttgctaagaa
    10201 tgtatgtctt tcactgactt gttcgtattg ttcgattgaa tttaagttta ctggtcccag
    10261 tttttcaatc tgttctttta aggatgaaac ctttgttcta ctgtcttcga tatcggttgt
    10321 ttcttgatag tcttgggaag ctttctcaaa tgtcagctga tattctgact gcagataggt
    10381 caaatgatta tctaaaatca tttcagctcg gtctttttgg acttctaatt tggattgttc
    10441 cgacagtcgt gctttttgct cacgattacg ctctgccaag actgcttcaa gcttatcgat
    10501 ctccttttgc cttctatctc gctgctcttt ggcttttgcc aactcggctt ttaatgtttc
    10561 tcgctgagca gataattcgt taatttgttt ttccaaactt tcttctgtga cttcatgatc
    10621 agaaaagtct gctgtcaatg tcgccaactg cttttctatc gcttcttgac gttcggttgc
    10681 ctctgctttt tggactcggg caccgcggag ctggatctgc agatgattaa actgctcttt
    10741 caagacagca agatctgctt gttcttgtgc tttttgactt tgtacttgtg ctcttctggc
    10801 ttccatctgg tcgctttctt gacttaacga tttgatttct tcgtcgattt tttgtcgttg
    10861 cgattcaaga tctgtttgtt tgtctttcaa ttcgttcgtt tgcttttcgt aatcttcgat
    10921 aaactgctgc agttcacgtg tctcaaaagt tgagatttgc ttttcttttt caaaacgttc
    10981 aagttcattt atgatgtttt gtagctgatt tgtcgcttct tgttcttcaa aacgtaattg
    11041 ttcgcctcgt gtacgcaaaa cttcttgttc ttcggctaaa cgagctgttt cttgttgcag
    11101 ctcctgaact tttttttctt gtgcttggag ttgtttatct gcttcttcaa attcactagt
    11161 caactgtttc aattcttgat tttgtacaaa caaactgcct tggtttcctc gtttattcgc
    11221 tccacctgtc atggatcctc cggcattcat cacatcacct tctaaggaaa ccactcgata
    11281 ttgatagcgg atcgtttgcg cgattgcatt ggcactagtt aagtctttgg ctaaaaggat
    11341 cgttcctagc aggttttgaa ccacagtttg gatttgatcg ggataactga cttgttcact
    11401 ggcaatccct ataaaccctt ctacagctgc tgcttgtgtc aatatatgag ccggtaattg
    11461 tcttggtttg attgtcgtca gtggaaggaa tgttgctctt cctcctcttt gctgtttcaa
    11521 atacgtgatg gcttgccgag catctttttc attttcaacg atgacatgct gggctgcccc
    11581 acctaaagct gtttcgatag ctaacgtgaa atctgcagga acatcgatca gttcagccac
    11641 tgctccgaca atacccgaaa gctgctgttt atgctgcaag accaatcgaa ccccttggta
    11701 aaaaccaaaa tagttttctt ggatttcttg aaggcttttc ttacgagcac gtagctgctg
    11761 gacttgattc attaattgat acattttagg ctgttctttt gctaatgctt cttggaactt
    11821 tttcccgttt tgttggatca tttctaactt ctcttttgtt tcagttaaag atgattttag
    11881 ctgcgcctgc ttttgtgtca attcttcttt ttgtaagatc aatcggtcta ctgatgcttc
    11941 tacttccgat tgcttggcta acgtctgctt gctttttgct gtttcttgta tatattgacg
    12001 ttctagatac ttcagttcgt ttcctacagc tgcttgttct tgcatcaaat caacatattg
    12061 atctcttaat tctgccaata attctttcgt tgatttttgg tatttttcta attcttgctg
    12121 tgtttttatg agatttgctt cagctttttg gatctctgtt tctttttctg ctgctgcttt
    12181 catcaagctt tcttgaagct tttcaaaatg tttaaccttt ttttgtgctt ctgctaatga
    12241 agtttgatat tcttgcgagc ttttttgtgt atgctttgtc cgctcctgca aaacatcttt
    12301 ttgtccttct gtctgcttca gtttttccga aagatctaat aatacttgct gatttttttc
    12361 gatcagacga tcggcttgcg cattttcttt acgctgcttc gccaaaatgg attcttgttc
    12421 ttggatcgac tcagatagtt tgcctaattc tagattgaat ttcgcaagct gtgcttgttt
    12481 gttgtcccaa tctttttttg ctgtcttgat ttcagcaacc atcaaagaga catcggtttg
    12541 agtcagtgtt tcttttagcc gtaaaaactc ctttgctgcc tcactctggg cggccaaagg
    12601 agttaactgt tcttccaatt cgtggatgat gtcttggacg cggctgaggt tgtcttctgt
    12661 ttcaaaaagc ttctgttctg ctttcttttt ccgctgcttg tatttcaaga caccagctgc
    12721 ttcttcaaag atcccacgac ggtcttccgg tttactactg aaaatcgctt ctacttttcc
    12781 ttgagaaatg attgagaacg attcttttcc taatccagaa tctaagaaca attcttggat
    12841 atcttttaat ctgcagcttt gtttattgat gaaaaactcg ctttccccag tccgtcgata
    12901 acggcgggtt acactgatct cattgtactc caatggcaga taatgatcac tattgtctaa
    12961 aatcactgtg acttctgcaa tattcagctg cttacgggta tcggaaccgg caaagatgat
    13021 atccggcatt tttccgcctc gcagactttt agcagattgt tctcctaata cccagcggat
    13081 agcctctgta atattgcttt ttccgctgcc atttggtcca accactgctg tgacgctatt
    13141 ttcaaaatca atgactgtct tgtctgcaaa tgatttaaaa ccggctattt caattcgttt
    13201 caaatacaaa gctttccctc acttactttt acagtgctgc caatgcattt tccgccgcag
    13261 cttgttctgc taattttttc gatttccctt gtccagtgcc aatcaattga tcatctacat
    13321 aaacttcgat ccaaaatact cgttcatgag ctggaccttc ttcattgatc aatcggtatt
    13381 cgattgatac atcacctgat ttttgcaaga cttcttgtag ttttgtttta tgatccatct
    13441 catgtgaaaa agcacctgct ttgactttag gaaaaatcac tttttcgatg aatgcatggg
    13501 tcgtgtcgaa tccctggtca agatataacg cgcctaaaaa agcttcgaat aggtcgcaaa
    13561 gcaatgctgg tcgcgtacgt cctcctgaat tctcttctcc cttgcccaac aaaacatatt
    13621 gatcaaagtg acattctttg gcaaatttcg acaagctgtc ttcgcgaacg atcgcagctc
    13681 gtgtttttgt caattttcct tctggcaaat gaggatataa acggaataaa tactgagaaa
    13741 ccatcagttc taaaacggca tctcctaaaa attcaagtcg ctcattatcc gataattgca
    13801 gattgcgatg ctcattcaca taggatgaat gggtaaaagc ttgttcaagc aaatttagat
    13861 cgtgaaaaac gatgttatag ttttctttta gttcttttgt cagctggttg tccattttcc
    13921 acattccttt tatggatatt ttttcgatac ttccttatta tactggtttt ttgtcaaatt
    13981 tgaaatggtt cggtcaaaag gtaagtaaag ctttaataat cctagtgatt gacgagaagt
    14041 tgtgaaaaat tcgtttagtt tcgagtatga agaaaaaatt ttgtgaaaat ggctttccat
    14101 atttttgcat aaatttgact taataccgag aaactggact ttgaacaccg tttattggag
    14161 aagtcatgaa atcagcgcct tgacctgaat attagagaaa taaacagaaa aatatcaaaa
    14221 aaccgaaaaa ggacatcgcc ctttttcggt catttttagt gattatgaac gtgaatctgc
    14281 tgtaacagaa acatttgccc atgggtttgg tacatcataa gaccaatccc agtctttgac
    14341 acggtcgttg actggcaata cttcattacg gaacaacgtt ggaatcgcaa atgcttcttc
    14401 aaatgcatat tgttgccatt tttgatatgc ttctttttgg tactcttcat caaatgcttt
    14461 gcttgaactg atatcgttga tcaattttgt attttcttct gattcataac gagtgtagtt
    14521 gaacgcagca tttggtcccc atagtccact tggatttgga tcagttcctg tattccaagc
    14581 agcttggtaa acatcaattt caggatcatc gttttctaat ttatcataga aagaattgaa
    14641 ttcgatcaga cgacctgttg tgtattggac atccaaacca atttctttcc attgttggat
    14701 atagtaatct gccaatggtt gcgctgtttc cccaccggac atcgaagcaa agttgattgt
    14761 tagtttattt ccatctttgt cttctcttaa accatcgtca tccacatctt tgtagccagc
    14821 atcatctaat agttttttcg ctttgtctag atcttgtgtg aatcctttca tctcatcatc
    14881 atggtatgac tggaatactg gaggaatcaa tgaagtcgca ttgcttctca aacctgcata
    14941 gaaacgttca cctactgcat tattatctaa tgcatagccc attgcttgac gtaatgattt
    15001 atcagccatt tttgaatctg gattgtattc cacactcttt gtatctgcat tccatttacc
    15061 taacttgaat cctagataag tataagaagt ttgttcacgg cctaagtttg tatagccagc
    15121 aatttcttta taggtatcgt aagtatctgt tggcatttgg aaaatcatat catataattt
    15181 agagttcaat gcttcagttg cacttgccat cggtacagat ttcatcgtga tctttttcaa
    15241 tttaggcgta ccattccagt agtgctcgtt tggcaagtat tctactgatt ctcctgcaac
    15301 gatattactc atgtaataag ggccaaaagt cactggattt ttacgtactt gatcgcttga
    15361 ttccatatct ttgattgcta caccttctaa cgtatgtttt ggtgctgcat atgaccagat
    15421 accaccaccc gattgaagca tgcttgggtt tacttcttta tattcgattt caactgtttt
    15481 gtcgtctatt tttttgattc cggagattgt gtcggcactt ccatcatgat attcttccat
    15541 acctacgatg ttacggaaag tgtcgtcata acggatacca gtgtaatctt tattcccaat
    15601 gatttcgtaa gggaaaatca catcgtccgc cgttacatct tcaccatctg accatttgac
    15661 gttgtctttt actttgattg tcgctttttt cgtatcttca tctagatcaa gcgttgcagc
    15721 tccgtcgtct gtgattctga aatcgtcatc gtttgcaaat agcggttcat gagaaggagc
    15781 cataaaagct gcatcatatg aatcttggta aaactcccac tggaacaatc ctttaaactg
    15841 actgtccgtt gcgaccgcag cttctaattc gccgtcttcg attgcatcat cattattttt
    15901 tgttgcaatt ggaaaagtta attcctcttt gttattgccg ttgcctccag agttgtttcc
    15961 agaggtatct gtacctccgc ccccgcatgc tgcaagaacg acagcagaca tcagcgctgc
    16021 aaatccaaaa atcgttttct tcattcctat ttcccccttc gcttctatac tagcctaacc
    16081 gttggcgcgc atctgcagaa cgcttgaatg cttgtccaac ataattgata ctcaacatca
    16141 aaactaaaat aaggattgat gcgggtaacc agatccattg ccgattcact aagacttctc
    16201 cgttacttgc ataaccgatc aatgtaccta aactaggtac atttgccggc aagccgaaac
    16261 ctaagaaagt caaagttgtc tcgataccga tgtttgccgc aaagttcatc gtcaaattag
    16321 taatgattaa tgaacttaga ttcggcatga tttcgcggaa catgatttta aaatcattgg
    16381 ttcccattgt ttttgaggca ctgacataat ctcttcttgc ttcagataat gtcttgcttc
    16441 ggaataatcg ggctttagct acccaataaa atgcactcat gatgaatata aaggaccaga
    16501 cattgtatct ggaaataatc gatacgaaga cgataatgat cagcatgatc ggtagaatca
    16561 tgataaaatc aacgatccgc atcaagatgt tgtcaatgat ccctccgtag tagccagaga
    16621 tgatccctaa tccaaccccg atgatcgaag taaggatcgt gatcgcaaat ccgatcgtaa
    16681 cagagtttct agcacctatg atcaattgtc ctaatacatc acgtccgcct tcatctgctc
    16741 ccagaatgaa cttttgtcct tgtgtcgaaa ctgttcctgg tgcagcgtat ttgtcgaaga
    16801 tactaacata catcactttg tcttgatcgg tcagcatcgc accgataaag atcactaata
    16861 ataaaaccac tagcaaacct aatgaaaaaa tagcaatttt atctttttta aattctcgag
    16921 caatcattcg tatccccata ggaggaatac tttcttgaac tgctgtattg tttttttcac
    16981 tcatcgatcc attctccttt ctgattggtt ttattgaatc cgaatacgcg ggtcaacgat
    17041 actcatgatg atatctgaaa gtaatgtccc cactagcgtc gcgattccta aaatcaatac
    17101 taaggatgtg atcacggtat agtcccgttg gccgatggaa tcaataaata gttttccgac
    17161 tcccggataa ccaaaaatct tttcgataac tacagaacca gcaatcaacg aagtgatttc
    17221 ataccccatt tgagaagcca tcggcaacgc tgcatttcgg aaaatatgtc gactgaatac
    17281 tttattcgtc ggtacccctt tggaacgggc tgttctgaca aaatcgagcg atttcgaatc
    17341 gattacttca cttcgcagat attggattgt gacggcagtc gccaaaaatg cttgggtaat
    17401 tgatggtaac agaatatatt tcaaacggtc tatccattga gtgataaatc caccttgcaa
    17461 acctagtgtt tgtgatccac ttgttgggaa ccagcctaaa cgataaccaa aaacaaatag
    17521 catgatcaag ccgaagataa ataaaggaac agcaaaacta aagaagttgt agattacgac
    17581 aatcttatcc aagattgaat tttgataccg gccagcaagc atccccagag gcacagcaat
    17641 caaataagta aggacaactg tcaagatcga tagatataaa gtgttgatga tccgttcgcc
    17701 gatcaagtca gcaacagagc gtttgtataa gaaactttga ccaaaatcgc cttgcaaagc
    17761 atttccgatc caacggaagt actggacata ccatgggtca ttcaagccag ctgcttgtcg
    17821 catctgttcg atcactgccg gatcctgatt tggattgatc aaaccggtaa atggatcccc
    17881 cggcatcgct tgggctaata agaagatcag aacacttaaa ataataactt gcgggatcat
    17941 caataaaaca cgtcgcagaa tcgttttcca cattagcttg cacctccatc tttcaacgcc
    18001 actttgtgtg tagttgtgat cgtacgcaaa tcgtagactc gaccagtcgc atcatagtat
    18061 tctttttggt tttggatata ctcttgttct actcttcgtc gattttcttt gtggctttcc
    18121 ctattttcta catcgatttg cggaatagct gaaagcagac gtttcgtata gatatgtcgc
    18181 gggtcattat aaatgtcttc tcttgtaccg atttctacaa atcgaccttt gtacataatt
    18241 gcgatattgt cgcacatgtg ttttactaca cctaaatcat gtgaaatgaa taaatagctg
    18301 aggccgtatt cttgctggat tcgtttcatg aaattcaata cttgcgcttg aacagataga
    18361 tccagagcag atacaggttc atcggctacg atcagttttg gattggtagc aacagcacgg
    18421 gctactccta gacgttgacg ttgtccgcct gaaaattcat gaggatattt atatagcgca
    18481 tcttccggca tcccaacgat atctagcagt ccttttacct ttttcttttc ttcttgatcg
    18541 cttaaccgtt caaaatttcg gatgggttca gcaatgatat ccaacactcg ctttttagga
    18601 ttcaaactgg acattgaatc ttggaagatc atctgcacat ccttgttata acccattttt
    18661 ttgcggttgc ttcgcttcgt tacatctttt ccttcataca tgatttgtcc ggctgttacc
    18721 ttttctaatc ccacaacggc ttttcccgta gtagattttc cagagccaga ctctcccact
    18781 aaaccatatg tctttccttt ttcgataata aaatccacac catctacagc atatacatga
    18841 tccgttactc gattgaagaa tccactgcga atagggtaat gaactttcag gtctttgatt
    18901 gtgattaatt ctgtcatcta cacctctcct ttttcgtctc taaagtggaa atgcttatag
    18961 caggagcaac gtaccaagtg gttcggtgcg acttcatgaa ggataggctc ttcttcatga
    19021 tcgcttgctg gtatccatgg aatacgtggt gcaaaccgac aacctttacg aggcatgttt
    19081 ttcagagaag gaacgacacc ttcgatcaca tggagttctg catcatgatt tccttcttgt
    19141 ggtatagagt tcagtaaaga gcgtgtataa ggatgcttag gatgatcaaa cagttctttt
    19201 acacctgcta cttcaacgaa ctgtccgcca tacatcactg ccactcgatc agccatttct
    19261 gctacgactc ctaagtcatg agtgatcaag atgatccctg tttttgtttc ttcctgaagg
    19321 tctttcagta aatcaagaat ctgtgcttgg atggttacat ctagggctgt cgtcggttca
    19381 tctgcaatga cgattggagg tttgcaggcg attgcgattg cgatgatgac acgttgtctc
    19441 ataccgcctg atagttcatg gggatattgg cgtccaactc tcgcaggatt aggaatccct
    19501 acttgttcta gcaattctag tgcacgagct tgacgctgtt ctgcattcat ctttgtgtga
    19561 taggaaagtc cttctttgat ctggtcttcg attctcatga gaggatttaa tgcagacaac
    19621 ggatcttgga aaatcattcc gatatcattt ccgcggattt tattatagag cgtttcgttc
    19681 aggtcgatta aattcagatc gttatataaa atctcacctg taatttttgt gttattagga
    19741 tcaagtaatc ccataatcgt agtagccaag gtacttttcc cgcacccaga ttccccaacg
    19801 attgctaaaa tctcgttttt gtctaaagta aatgaaacgt catctactgc atcgtaatag
    19861 tcatctttga gtcggaatcc tgtatgtagg ttctgaactt ctaataattg gttatcggac
    19921 aatcgaatac ccctctctgc ttcctaaatg gaagtttttc ttaataaaaa aggacattac
    19981 acagaataaa tcgttttata acaaaggtta ttcttatctt tgtgtaatga a
//
LOCUS       DAGCYL010000035        19641 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.036, whole genome shotgun sequence.
ACCESSION   DAGCYL010000035 DAGCYL010000000
VERSION     DAGCYL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.036"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(377..1321)
                     /locus_tag="MB216_000971"
     CDS             complement(377..1321)
                     /locus_tag="MB216_000971"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBT4525825.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1618..2562)
                     /locus_tag="MB216_000972"
     CDS             complement(1618..2562)
                     /locus_tag="MB216_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4525826.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKFRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2782..3975
                     /locus_tag="MB216_000973"
     CDS             2782..3975
                     /locus_tag="MB216_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4525827.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYVTRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAGQLVQLGWQYTFLIYA
                     ISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIVVYNAVSI
                     KLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSLLLMGIGF
                     VLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKHAESFNTSILLVGANLGT
                     FIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIRVDEEIKE
                     QLTTK"
     gene            3997..6012
                     /locus_tag="MB216_000974"
     CDS             3997..6012
                     /locus_tag="MB216_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBT4525828.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TETGGGILFDFGRAVNGCVELISLDDHIESITLCYRESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6095..7468)
                     /locus_tag="MB216_000975"
     CDS             complement(6095..7468)
                     /locus_tag="MB216_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBT4525829.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7494..7778)
                     /locus_tag="MB216_000976"
     CDS             complement(7494..7778)
                     /locus_tag="MB216_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4525830.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7780..9795)
                     /locus_tag="MB216_000977"
     CDS             complement(7780..9795)
                     /locus_tag="MB216_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBT4525831.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     THIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFDSFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDDPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9974..10279)
                     /locus_tag="MB216_000978"
     CDS             complement(9974..10279)
                     /locus_tag="MB216_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBT4525832.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHY"
     gene            complement(10368..11399)
                     /locus_tag="MB216_000979"
     CDS             complement(10368..11399)
                     /locus_tag="MB216_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4525833.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNILVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIKSGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTTNSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11606..11794
                     /locus_tag="MB216_000980"
     CDS             11606..11794
                     /locus_tag="MB216_000980"
                     /inference="COORDINATES: protein motif:HMM:NF013577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4525834.1"
                     /translation="MLIEEKLTKQELFTTTEKRIADYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKNGIQRI"
     gene            11775..12482
                     /locus_tag="MB216_000981"
     CDS             11775..12482
                     /locus_tag="MB216_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4525835.1"
                     /translation="MGFSGFKEFRFELGWEVHQLIAQFNQTIDPNFPFSSEDSTQQIA
                     KKMADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKL
                     NKFLIMAEEYSDSSWNAANLISEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGN
                     QHSEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLK
                     NKQELMQKGLLKEYRKT"
     gene            complement(12634..13227)
                     /locus_tag="MB216_000982"
     CDS             complement(12634..13227)
                     /locus_tag="MB216_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4525836.1"
                     /translation="MHNKIHNKERLLIWAFFLKQIQLTIILDHNFFDLLFFDWKWQRA
                     SSVLLEGTSYPTGGENTHAVISSHRGLPQAKLFTDLPKLTLGDSFYIEINGRTLAYQV
                     DQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMVNSHRLLVRGHRVPYHAKEVQKEVQK
                     VSENKRFFLYGLLAACILIFLLLIYLFRQSFKIRNRS"
     gene            13231..13959
                     /locus_tag="MB216_000983"
     CDS             13231..13959
                     /locus_tag="MB216_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HBT4525837.1"
                     /translation="MRESQGTPFEWKAGDSYVQLINIDKKDFLILKNLLGKLKYSSYI
                     KVVNYFVNTIFSEFSEKEKNEISEQIRKNMSYCSFSSSDCKEKNEEYVVQLLTQIYFF
                     SRIFPYDTTHLTICATYFAQQMQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSSYIFW
                     MLIGNPEIHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITEDQLSK
                     YNLLEYDLNVSFANEEELIRLFEL"
     gene            complement(14032..14679)
                     /locus_tag="MB216_000984"
     CDS             complement(14032..14679)
                     /locus_tag="MB216_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBT4525838.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKIGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNKKWLELLRI
                     NHFSIQSSEEAVSFLYFSMSLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(14687..15373)
                     /locus_tag="MB216_000985"
     CDS             complement(14687..15373)
                     /locus_tag="MB216_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4525839.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(15379..16452)
                     /locus_tag="MB216_000986"
     CDS             complement(15379..16452)
                     /locus_tag="MB216_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4525840.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEEGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHELKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            16675..17349
                     /locus_tag="MB216_000987"
     CDS             16675..17349
                     /locus_tag="MB216_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4525841.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIETLRNVG
                     YILQRV"
     gene            17354..18694
                     /locus_tag="MB216_000988"
     CDS             17354..18694
                     /locus_tag="MB216_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4525842.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMATIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            18783..19641
                     /locus_tag="MB216_000989"
                     /pseudo
     CDS             18783..19641
                     /locus_tag="MB216_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         6159 a         3652 c         3545 g         6285 t
ORIGIN      
        1 ttttccatca aaatctgctt atgcaaaatt ataaaaaaat tttgcagttt ttcttacttt
       61 ttttgtcgtt taattgaatg ataaaaacca gtttctttat tcagttaatc aaatatgaac
      121 gaattagata tgaatcattg tatttgccgt tgcagaagga ttttttaatt aaccacagat
      181 aaacattgat aacgattcgc ttattgtaat caacagatta aatttcatat gttcatatgt
      241 acggatacat ttttggactg attaatttta accattctgt ttatttgctt acctattaga
      301 tatcaataaa aaactgctta acagctattt cagctgttaa gcagttttag gatttttagt
      361 tcatattcat tgtttcttat aatacagtgt tcaatacttc tcttaaagca cttgttgatt
      421 tgctgaacat tgctttttct tcttcgttga ttgataattc aacaacttca cgaacaccat
      481 tttcgtcaac gattgatggc acacctgtaa agatatcttt ttcgtcgtat tcaccagtta
      541 aataagcaga tacaggcaat acagcttgtt cgttattcaa gatagctttt acgatacgtg
      601 cagtactcat accaatacca tagtaagttg cttttttacg atcaatgatc tcataagcag
      661 cattacggac tttatcagca atcacatcta attcatcttg ggcaatacga tggtcttttt
      721 ctacaatttc aaacactggt ttaccaccga cagttgtatg tgaccatgca gcaacttctg
      781 aatcaccatg ttcacctaag atatagccgt ggacgctgcg tgggtcaact tttaatttca
      841 atgcgatttc tttacggaaa cgagttgtat ccaatgttgt tccagtaccg ataacacgtg
      901 atgttggcag accagattcg ttccaagcga tatatgtcaa aatatctact gggtttgaag
      961 caacaacgat aataccgtcg aatcctgatc ccatgatttc ttttacgatt tggcgcatga
     1021 ttgacgcatt tgttttaact aaatctaaac gagattgacc aggtttttgg ttgataccag
     1081 ctgtgataac aacgatatca gcatctttac actcttcata gccacctgac catacagcca
     1141 cattttcatc gccccaagcc ataccatcta aaaggtccaa tgcttcccct tctgcttttt
     1201 cttgattcac atcgatcaat actaattcat tagagattcc ttggttgatc attgcataag
     1261 cgatacttgt tccaacaaaa cctgtaccga cgattactac tttacgactt gtttttttca
     1321 tgaataaaca gctccttttc ataactattt gttactttca tgaaaagtat agtcttgttt
     1381 attcatttta tcaacatgtt aattataata gttgttatta taatttttga tggaaacgaa
     1441 ttcctgaata cgcataacag ataaatgaca aatgtttaca cctatatccc ctgataatac
     1501 aaccaaaagt ttagaactga aaataaatcc aaaaaaaatt cgctgcaacg atataataag
     1561 acagcaaccg cacctaaaac aattttcctt catatgcttt agtgaaagat tttcttctta
     1621 atgtttattt ctccattcat tgggagtaca tgttgaatac ttcttgaata atttgaataa
     1681 agaagtcgtt tgtttatatc cgatacgatt agcgatttct tccaacgaaa aactcgtttc
     1741 ttgaatcagt tccaacgcaa agtcgaattt tttctttaga accagttctt gaaaggaagc
     1801 atcaaaatac attttcaatt tattactaag atagaccgga ttgtagccaa aatgagctgc
     1861 tgtttctttt aacgtacaag aagtaaaatg cttatcgata taatctaata ctctaagcat
     1921 ctcattttct gcgcatccat ctgctgatga aaagctttgt tgttctaata aatgagtcaa
     1981 ttcgatcagt aatagtgatg tatagagatt taacgaacgg aacttatgac tgtatgtatc
     2041 aaaataaatc aacagcatat tttcaatcaa atgcgtcaaa cgacttgttt ttccgccgcg
     2101 aaaatgaatg aattgtgtat gttccccttt ttcttttgat gcattgatca aaaaagaaga
     2161 aagtagatta tttgtgcttg ccaaatgatt caatatctct gtagataagg tttcttcttt
     2221 taataagatg ttgattaaaa tatcctcttg ccctgtgtat tcgattcttt gagtagcttc
     2281 tttatcaatg atcaccacgt cactttcttc catctctatc ctttctccat tgataatttg
     2341 taaacatgta ccgctataca tataattgat ttcaacaaaa tcatgtgtat gctcgggaac
     2401 ttccgcatat cgacggtgtt tactgatatg aatatttccc tgttgaaata gatctcctcg
     2461 gtggattttt ggcggtgtct gaagatggta agctaagttt aaatcattga tattcattcc
     2521 ttgttcacgc tgcagagttt cgattgattc ttcgttacgc aaaaaagtgt ctaattcagc
     2581 tagttccaat tgactccctc ctttttgtaa gtggtttctt tcgcttatta gtttacgctg
     2641 tactgctatt atatgcaaag ctatttttgg tttcataaag actttctatg gttgggacat
     2701 gaacttcctg tgattccgct attgagagaa taaccacttc atactatatt tttgataagc
     2761 gcttacaaaa atgaaaggaa catgcttatg aataaaaaga tgatgaaatt ttctctgctg
     2821 tcagtctctc ttattctgac ttctgctggt gcagtcacgt ctgcaattcc cagtatgtca
     2881 gctacttatg tcacgcgttc tttatctgct gttgaattat taacgactgc tccctccttt
     2941 atggtcacta tttttgtttt gattagtagt tttattgcta ggaaaatcgg acaaaaacaa
     3001 actgtgatca tcggattagt gattgcatcg atttgtggta tcctccccct cttctcacaa
     3061 agttacagta ttgtcttagc ttcaagagct ggactaggta ttggctttgg attgatcaat
     3121 tctttggcag tcagcatgat tgctgccttt tatcacggag atgaacgagc ctctatgatt
     3181 ggttttcaaa gtgcatttca aggatttggt gcaggacagc tagtacagtt aggttggcaa
     3241 tatacctttt taatctacgc catcagctta cctattcttg tcttgtttgt tttgtttgtt
     3301 cctacacctg aaacaactga acttcaatca catggcacag ccccaacaag acaagttatc
     3361 aaaacgaatc ctaaaatgat tagttatggt atttttctct tgctggtcat cgtggtatat
     3421 aatgctgtat cgattaaatt agccacagtc ctgacgactt attctctagg aaacgaaact
     3481 aatgcagcga atatcttaac aatcatgcaa ttggcaagta tggctgccgg agtttgtttt
     3541 ggctggcttc agacaaaagc acatcgctat atgcttacac tttctttact actgatggga
     3601 atcggctttg tattgattgc cactgtgata aacctgtatg tgattgggtt tggtgcgatc
     3661 ctaacaggaa tttctttttc gttattcatt ccttacttat tcaaccaagt aagtatccaa
     3721 gcgcctaagc acgcggagag tttcaatact tcaatcttat tggttggcgc taatttaggt
     3781 actttcattg caccatatgg tttgctcttt ctctcttttg cttctcttgg gagtaaaaca
     3841 cttgctgttt tcgtcaatgg cagtatcttg ctgttgatta gcgcgcttat tagccttcta
     3901 gtagtacaca ggacgaatag aaagacagtg ataagagtag atgaagaaat aaaagaacaa
     3961 ttgacaacaa aataaatttg atggaggtcg tgtaaaatga caaatgaaaa acataaagat
     4021 agatggcttt ggtatcctgg agactttgag atccgccatg gactgctaca aaatttccaa
     4081 cgggaagaac gaggttttga ctggccagcg tactggtaca tggatgattg ccatcggaac
     4141 gtcaagttta agagatatta ctttcttgat caaccttcca tgtttaaagt gacgatccaa
     4201 ggagttgggt atgtagaaat taatggacaa aaacatccat gtggaaaatg gctgacttgt
     4261 ccagctggta aagcaaaaat tcgtatcttt gtaggacata catcagggct accagcgatg
     4321 tttatcgagg gagacgaagt aaaatcagat attggctgga cagcaagtaa ttttattgaa
     4381 gaatatccag ctggctggag tccgctttat gtcgatatcg caaaagaccc aaatcaaatc
     4441 tattatcaaa aagaatgcgt ccatccagtc actgaaacgg agactggcgg aggaatctta
     4501 ttcgattttg ggcgagcagt gaatggctgt gtagaactaa tatctctgga tgaccacatc
     4561 gaatctatca ctctatgtta tagagagtcc gatgtagaag ctatggatat cgattattgc
     4621 tactataagc aaataggaat gacaagaaaa atgaaagctc gtaaacgcgc ttttcgctat
     4681 ttgtttatac cagaggtttc tactggaatg gttcaacttc aagtttttca tgaattttta
     4741 tctctttcga aacaagctgc tttttcaagc gattcttctt tactgaatca gatctggtca
     4801 gtatcagaag aaaccttcaa gttatgtagt gatttgtttt ttatcgacgg tatcaaacga
     4861 gatcgctgga tttggtctgg agatgcgtat caatcattca tgatcaatca atatcttttt
     4921 tttgacgagg aaatcaataa acgaacgata ttagcattaa gaggacaaaa tgaaatcaag
     4981 cagcatatga atactatcgt tgactattcg atcttgtggt tgattggtat cgaaaaccac
     5041 tatatgatga cgaaagacta tgaattttta cgcatcgtct accctaagat gcaatcatta
     5101 atgagatact tgatggagca aacgaatgaa ttagggttta tttatgggag agcaaaagat
     5161 tggatattta ttgattggtc tgaaatggat aaagaaggaa cgcttgctgc agaacaagtc
     5221 ttattactga aagcctatca ttccatgatc acttgcggag atatcttagc tgaaacatat
     5281 ggagagaacg aaaattatca tactcatctc tatcaagaaa aatatcaact attgaaacaa
     5341 aacctatttg actacttttg ggatgaagaa cttggcgctt ttatcgattg ctatgaatct
     5401 gggagaagaa atgtgacgcg tcatgcgaat attttcgctg ttttattcga ttttgctaac
     5461 aaagaccaac agcaatcgat cttaaagaat gtcttattga atgacactat cacacagatt
     5521 actactccat atttcaaatt ctatgaacaa gatgctttat gcaaactcgg acagacgaat
     5581 atcgtctacc aaacgattct tgattattgg ggagggatgt tatctcatga tgcggtaact
     5641 ttctgggaag aatacgaccc tactcaaaca ggaaatgaaa agtacagtat gtatggagat
     5701 ccttatggaa aaagcttatg ccatgcatgg ggagcaagcc caatctatct tttaggacgc
     5761 tattttatcg gtttgtatcc aacaaagcca ggttatgaag aatttatgat acaacctaat
     5821 ctagattcat ttgaaacact gaattgtacg ctacctatca aagatggaaa gatttcgctt
     5881 gaaaaaacaa aagataagat cgtgattact tctgatagaa caggcggaac agtgaaaatg
     5941 aatggtaaaa gttatcctct tctagcaaaa cagccacttg tcttaacaag ctattcaaaa
     6001 ataaatgcat aaaacaaaaa actgcttgcg acatatcaga agactttaaa tcttcttctg
     6061 atattgtcca agcaattttt attttgaagt ttgcttaatt ctgttcttta gaaacaatgt
     6121 ttgttctttt tccggcaaaa agaaagctag ataatacgac aagtaaaaag aaagcgatga
     6181 ttccaactgt caaggctatt tgaccgtatt ctcctagttt cccaagtaca agtcctggta
     6241 ctagataatc agtatcggca aatgtagaag aagcgatttt aagatctcct aatactggaa
     6301 ggataaataa tggcagccaa ctaatcaata agctattgaa agcagaacct aataccgccc
     6361 ctcttctacc gccagtcgag ttgccgaaga caccagaagc agctccgcag aagaaatgtc
     6421 ctactacccc aggaatgata actgtagttt gcaagcctat catagcaaac atggatagtg
     6481 ttcctacaaa gaagctaaca aaaaagccaa ttaatacagc attaggtgca tatggaaaaa
     6541 cgataggaac atctaatgct ggtttagaat cgggtacgag ttttttagca atcccttgga
     6601 atgctggaac gatttctgcc aacaccattc gaaccccttg aaggacgata acaaacccag
     6661 ctgtgaatgt tccagcttgg atcagtgcaa agattacagc gttgtctcca ttgcttagtt
     6721 ctctttcaac aaatccagga ccagctaaca aagcaagtgc gacatatacg atcgacatca
     6781 agagcatgat cgatacagta gaatctcgca aaaagctgaa acttttagga atctttatgt
     6841 cctctgtaga acggtctttg tttccaaaaa actttccgat ccatccacta gaagcataac
     6901 cgatatttcc ggtatgccct aacgcaactt gattgtttcc agtgatgttc ttcataaacg
     6961 gttgacaaag agcaggcgta atagttaata gtgttccttc gaagaccccg cctaataaga
     7021 tcatccacaa atttgcagta aaccccgcgc ttactaaaat gactgctgtt agacaagaaa
     7081 cataaagcat tgcttgacct gtaagaaaaa tgaatttata tcttgtgaac cgagcaagta
     7141 aaatattcac gatcatccct actagcatga tcaaagcagt aggtgtaccg tattgttgca
     7201 aagcaatcgc cactactgct tcattgctag gaactactcc ttgagtatgc agagctgttt
     7261 ggaacatcga agcaaaaggg gttagtgaat tactgattag accagcaccg ccgatcagta
     7321 ctaggaatcc gacaaatgtt ttgatagttc cttggatcac ttctgaaaat ttcttcttct
     7381 gaagaacgag accaagcagt gcaatcaaag aaacgaggat ggagggctga ctgacgattg
     7441 acacaaacaa attcaacaat ttatccattg atagttcctc ccttgaatta tttttattct
     7501 tttgacaaga tcttcgttac tttttctttt acttcttcgc tgtcaatgat cgattgcaaa
     7561 ggtacgagct tttcatcagg tatagaagtt agcgtatctt tcaaagaatg ctcaatgaag
     7621 aaataatcag ccatatcgct agatacactt ccaacatccg tatgacttac ttcaaactcg
     7681 tccacatctg ctccaagatc tgcaagtgct tgtttgatgt tcatctctac cataaaactt
     7741 gttcctaatc cactttgaca tactgcaaaa attttcatat cattcgtctc cttttcttat
     7801 gatcgctaat atttcttctt ttgttgatgc gtgtaataaa tcttgaagat tttgtttatt
     7861 agaaagtagc tttgttaaat ttgccaacgc ccgaagatgc gcctcattat cgatagcagc
     7921 taaacagata aacaaactaa tgggatgggc aggatcatca agcagatcca caggttgttg
     7981 aagtttcagc aaagacatcc ctacttcatt tacgccatct tctggacgag catgaggtaa
     8041 tgcaatgtgg tcgccaatat ggataaacgc accatactgc tccacgcgct cgatcatagc
     8101 tttgatataa ctttcttcta ttttattttc tgataatagc ggctgtgcgg caaatgtgat
     8161 tgcttcttgc cagttttttg gttgatccga taattggatc atttcttctg ttaataattc
     8221 agatagcata ggacgttgat cctccttctt ttctaaaagt ttgttcattt tacgatttaa
     8281 tacttgatag agcttttctt cgtctacgcc ttcttttaat tcgatataag gcagtagcgc
     8341 cgacaacaat tcttgggcag aaggcattga aaatcctggt atcagccaat ctctttgtac
     8401 ttgattgatc agctgatttt tttccaagtc agtcatgatt ggatgcagaa catagagaga
     8461 ctttttctta tcagtaagtg tgagaggtac agtagaaaaa ataatatcgt aagaagtttc
     8521 gtcaacctcg tgtaagcggt cagctgaatt agtctctgta aaaaagatca tcgggaataa
     8581 ttgctgaagt tcacttttta agatcaatga agagctaatg ccacttggac agacgatcac
     8641 ggctcttatt tttctatttc tttcttctgc gtccgctttt ctgatctctc cgccaaatag
     8701 tatagtgaaa aaaccaatct cttcttcagg gatgctcttc cctattcgtc tttctaacgg
     8761 aagtaatgct tttcttgtca tttcgtagat ggaagcatat tgatgcttaa tttgatcgat
     8821 catcacatta ggtaaataaa aagaatagga aatacgaaaa taggcaggga ccaagtgata
     8881 aaagacattc atcaataatt cacgaaacga atcaaattct acggccatca ggaattccat
     8941 gcgatggatg acttctgaag aacattccag caaaaaatca agtgcaggct cctggacatc
     9001 tccttcaacg atcgatgcta agcaaagaga gaaaaatata atctccgaat tttgatgttc
     9061 ggaatagcca aggccagtca atacttgtcg actaatgtca gtgactgcaa gagaagccaa
     9121 cagttcagca tcttttacca gttccatgga tgtttttcca attcgaatgg ttgtcaaact
     9181 caaataataa gctaattcat ccattcgatt tggtgcgaat cgaagatctg atagtgaaat
     9241 cgttttatct agcaaatctc taactttggc gtaataatca atggaaaggg ttgcggttaa
     9301 atacgtgagc ccaaatcttc cagcaagtga ctgcatcaaa cgagcagtgc gattttttgc
     9361 ttctcttctt aatattgctt ctgaccctag cagcctaaaa ccagttttcc tagtatacga
     9421 cagctgacaa tcttccactt ctaattcatt tcttatcttt ttgatatctg acaggactgt
     9481 ccctttagaa acctgaagaa aatcttgaaa atgaaaaaca gaaacagacg ttgcagtcga
     9541 ataaaccatc aaataaagaa gatctcttcg ctcttgttcg gtatacacag cttcaaatcg
     9601 agtaatatcc aaaaattttt ctgcccagtc gattgctcgg aatttcttta tacggatatg
     9661 ggtatcatcc acatattcgg caaaaggagt taattgtttg atcgcctcgc ttgtatttac
     9721 ttcgatgatc tctgctgctt tttctagact gatttctctt ggtgttttca ggagataact
     9781 aattttgtag ttcacagcgc tgttcacctc ctacttatat tctagtatgg tagcgctttt
     9841 aaatcgaggg gtgtttggta caacttacag ttggattttt tttacgaatg taccgaacga
     9901 cttctatcga atggatacag aaaaaaagca ggtgaccaaa tgtctcctgc ttttatgaat
     9961 tgatttcgtc cgatcaataa tgatcatgtt tattttttga ggtcagttct tcaaattttt
    10021 ctttgacatc ttcaaccgct ttttcgatat ctttttcaaa gtgattgggc tcattaggct
    10081 ttgtttcctc ttctacagaa tttgaaccag ataaagctgt ttctttattt tctgtttcac
    10141 cagagtttgt gccagctaat gattcttctt tgtcttctgt ttcaccagaa ttagttcctg
    10201 ctaaagccac ttcttcttct gttttagggg ccattgaacc tcttaatcgt ttgtgttttt
    10261 caccgtcata atttgtcatg ttggttcctc ctagtagttc tatacttttt catcgtacaa
    10321 taagacagtt tatgcgtcaa aaaacaaacc cgattcttac aaatatgtca tatttttcct
    10381 tctgattctt gaatgctttt gattccttct tccaaaattt tcatttgtaa gattgtttct
    10441 tcatctttga tgactttttc tgcgccatta actaatgtct cgtaaatccc ttcgtagaca
    10501 cggctgtaat caccaacctc ggaaattact ttttcttcat gataactccc ttgttcatct
    10561 atataggtca atacaccata atgttcgggc aaatcgatac caaagtcttc atgatcaggc
    10621 atgtaaaaca ttttcagatg ttcttcttgt cgatcttttg tttgtttcac gaacatccct
    10681 ttttttccgt aaacgacgaa acttggacgt tctttgatgc ggaaataact ggattttatc
    10741 gaaattttca tatttccata gaaaaaatcc agatcgaagt aatcattcat tcgtcctttc
    10801 cctaaaagtt gacgtacatc atagctgata tcatctggtt cgccgaaata agaaagaact
    10861 tgatctactg tatgacaagc atgtccatat aaatagctgt tcgtagtaga aaattcatgc
    10921 gtattttctg gtacttccgg tctgaaataa tcataatgca tttctacttc taaaatatcc
    10981 cctaattttc cgcttttgat tactttttgg actgtcaaga agtcagaatc gaaccgtctg
    11041 ttttggtaac attggataaa tagccctttt tcttttgcta atgcaaataa ctcttctgct
    11101 tcctttgacg tttctgcaaa aggtttctct actagtatat tcttaccatg ttcgagtgcc
    11161 atttttccat attcataatg aaattgacta ggtgtagtga tgacaactaa ttggatctca
    11221 ggatcatccc aaagatcaga cagctgatca gtatattcga tgtcttcgaa tgttttccac
    11281 actggttcag atggggaaaa aatttttttg atattgattt tgttcgtaag ttttcttgaa
    11341 aaaggcaaat gatagcgatt tgtactcttt ccattgccaa gataagcgat tgtaagcatg
    11401 agtggtgcct ccttttattg atgttttcag tatatcctag aaaagcgcag aaagtattta
    11461 taatagctat tctggtaaaa aagttcagaa aaacacttgt tattattcgg tttgatgaaa
    11521 ttaatgttca aagtaaggat aaaaatcaaa aagatagtag cggaaccaag tgtttatcag
    11581 taagatagaa tttggaggtc agatcatgct tattgaagaa aaactaacaa aacaagagtt
    11641 gtttaccacg acagagaaac ggattgccga ttacatccgt aggaatatcg aagctgcagt
    11701 ctacatgacg attgaagaat tagcaaaagc tacgtatact tcccattcag ctatcattcg
    11761 tttatgcaaa aaaaatggga ttcagcggat ttaaagaatt ccgtttcgag ttagggtggg
    11821 aagttcatca attgattgct cagttcaatc aaacgattga tcctaatttt cctttttcaa
    11881 gtgaagatag cactcagcaa attgcaaaga aaatggcgga tttatcgatc cagtcaataa
    11941 aaaaagcaca attgcagatt gaacatgaag atttagatca aatcggtaaa gtattgaaca
    12001 aggcacagcg aatctttctt tttgcaaaag gtgattctca aatcacggca aggaaattcc
    12061 agaataaaat ggtcaagttg aataagtttt tgatcatggc cgaagaatac agtgattctt
    12121 cttggaatgc agccaatttg attagcgagg attgcgccat ttttattagc tacagcggtc
    12181 gcatccatca ttatgaacga ataatgacct atttgaagca tgtagaggcc cctaccttgc
    12241 tgattaccgg aaatcagcat tcagaaatgg ccaaacaggc tgatatgtgt cttgttatat
    12301 cgcaggagga atatgatttt gctaaagtag caactttctc ctctcaaatt gccttcgatt
    12361 acgtattaaa tacgttattc tcagtgatat attctcaaaa ttttgaagag aatatccgca
    12421 acttgaaaaa caaacaagaa ttgatgcaaa aaggccttct taaagaatat cggaaaactt
    12481 aagaaaagat cctcatttag taatgaggaa gatagtgttt gataggctct ctttgatatc
    12541 atcataaaaa caggctgaag ttttcttttt caaacttcag cctgtttgtt tacggaacga
    12601 ttcgaataga tatattatag aaatgaggaa tagctaactt ctgtttctta ttttgaacga
    12661 ttgacgaaaa agatatataa gaaggaggaa aatcagtata catgcagcta acaatccata
    12721 caggaaaaat cgtttgttct cggatacttt ctgtacttct ttttgaactt cttttgcatg
    12781 ataaggaacc cggtgccctc tgacaagtaa gcgatgactg ttgaccatat aaggtgtaca
    12841 agttaggagt gtcacgaaat cctgcccttt ttcgattctc agatcttttg tatcagtcgg
    12901 ttctaccgtc ttgatctgat cgacttgata agcgagtgtc cgtccattga tttcgatata
    12961 gaaagaatca cctaaagtca gtttcggtaa gtctgtgaac aactttgctt gaggcagtcc
    13021 tcgatggctg gaaataacgg catgtgtatt ttctcccccc gtaggataag acgttccttc
    13081 caataatacg gaagacgcac gctgccattt ccaatcgaaa aaaagaagat caaagaaatt
    13141 atggtcaaga ataattgtca gttgaatttg tttcaggaag aaagcccata tgagcaatct
    13201 ttctttatta tgtattttgt tgtgcatctc atgagagaat cccaaggaac tccatttgaa
    13261 tggaaagctg gagactctta tgtacagttg ataaatatcg ataaaaaaga cttcttaatc
    13321 ttgaaaaatc tcttaggaaa attgaagtac agcagctata taaaagtagt gaactatttt
    13381 gtcaatacga ttttttcaga attttcggag aaagaaaaaa atgaaatcag tgagcagata
    13441 cgaaaaaata tgtcctactg ttcgttttca tcctctgatt gcaaagaaaa aaacgaagaa
    13501 tatgttgttc aattattaac gcaaatttat tttttctccc gtattttccc atacgatacg
    13561 actcatttga cgatctgtgc aacttacttt gcacagcaga tgcaacaccg ccagatacgt
    13621 ctatatgctg gatttcgtgc agaacttcaa ttttattcca ctctactgaa tcagaatctg
    13681 atttattatt tatctagcta tatcttttgg atgcttatcg gtaatcctga aattcaccat
    13741 tatacttctg ataaaaaaat actgatcatc agtgacttaa gtcttcgaca tagtcaatat
    13801 attgaagaat atatcagtga tattcttgct gtgcataaaa ttcactctga gacaactgct
    13861 attactgaag atcagctgtc gaaatacaat cttctcgagt atgatctgaa tgtgtcattc
    13921 gcaaatgaag aagaactcat ccgcttattc gagttatgaa taatcaaaaa acagctatgt
    13981 ataaaccacc ctgataaaaa tttcaggaca aatacacata gctgtttctt tttattgtat
    14041 tccttcagca atccaacgag atttatagcg aaagtctttt aacaactctt cttttgtcca
    14101 attatttgca atgagatccg tcaatgtgtt cattacgagg gacatggaaa agtataggaa
    14161 actaactgct tcttccgacg attgaatgga gaaatgattg attcttaata attccagcca
    14221 ttttttgttc attggagaat cagccgatag ttctctacgt ttggttgtct gctgatcatt
    14281 tcgctgggtt aacaagtata acctttgcca atagatactt tcgtgatcta agtcgcagca
    14341 ccactctaaa tatttttcga tccccaaaaa gaaatgttgt ttgttttcgc taatgaattt
    14401 taagatatct tgatggacta tgtgagaact gcttttgatc gtatacaaat aagcatcttc
    14461 caaatcttga aaatatttat aaaaagcacc gcgcgacatc cctatcttgt caacgatctc
    14521 tgagactttg acttggctga tgtgctgtga gcaaaatatc tccagtaata ggtcatcgat
    14581 tctctgtttc tttgcgttcg ataaattgaa aaaagtttct tttggcatcc acatctctcc
    14641 tttatctctg attatacgtg gatagccctt cattgtcatg aaggattcat tccttctttt
    14701 tctcttcttc tgtcaagtac ccatcttcca ttcgataaac gcgatcactc cattgaatca
    14761 tacggacatc atgagtaacc ataatagtcg ctttttgttt ctcatgagct tctttaacta
    14821 gtaatttgac gacttcatat gcatgatctg tatccaaact agctgttggt tcgtctgcta
    14881 agatcaagct tggctcatta tataaagcgc gagcaattgc cactctctgt ctttctccac
    14941 cagaaaggtc tttaggataa ctatttttaa gtgactggat atctaaagat tgcagcaagg
    15001 aatcgatttt tgttgaatct tctttttttg aagtaacttg gtcaactagt tcaaattgtt
    15061 cctttaccgt taaaaaagga atcaaattag agctttggag aataaatcca atttctttaa
    15121 agcgtaaatc tgctcgtttt ttttctggaa gctcagttag agagtgaccg ttgatcacta
    15181 tttctccgga agtcggtgtt tgcaatcctc ctgaaatggt aaggaaagta cttttacctg
    15241 aaccagaagg accaatgatg ctgacgaatt ctccttcctt tacataaaaa tcaattgctt
    15301 tcaaagctga gacctttgta tgaccagaac caaatgtttt ggtaactttt ttcatcgtta
    15361 atattttttt catttttatt aacctcctat tgctgtaatt gggtctactt tggttatcgt
    15421 acgtatcgaa aacaatccgc ctagcattgc aacaatgatc aaaatcccac tatatagcaa
    15481 ccattgtgac caaaaaacag caaaaggcat cgcgcttggt aaaatcagac tagtcaaata
    15541 agcaaacaaa agagcaagca gaacgcctac caccccaaca ataaaggact gtgctactaa
    15601 agatttagcg atgaagaaat ttttgatccc ttgtgctttc attactccaa aaatagcagt
    15661 cttctgaagt gtcatcacgt acatgaaaat accgaccaca gcagcaacca ctaagaacaa
    15721 aaagtatatc atcgcattta aggtcatatt ttgggcagaa tagccaggaa tactttcaat
    15781 gaatgtccca atagttaata actgtagacc cttcgatgct ttttctttcg aaattttagc
    15841 tgaatccttt attacgatcc cattgatcgg ctgttcttct tcagaagcaa acggctgttg
    15901 tccatacttc aaagcagtcc acgtatctag ggacgcatag atgacgggag aaactgtgta
    15961 ataagtttca ggaaagatac cgacaatcgt caaaggatcg tcgtaactgc caattttgat
    16021 cgtatctcca attttatagc ctgaatcagc cagattttga gagatgatga tttcattctt
    16081 tgttttaagt tcatgccctt tcgtgagttt tggtaataaa aatgcttctt tcgttgtgcc
    16141 gaaaactgta acattttctt tgttttttcc tttgattgct ccgctgtaca atccgattgg
    16201 tgctttctct ccttcttcaa tgtatttcag atctcctctt gttaattgtg aagctgcaaa
    16261 tacttgattt gcatcctccg ataaaacaat ctcttgtgcg tcccaatcat cgattgcttt
    16321 tttaaactct tctgaaagtc cattcgctag tcctgacaac atgaatacga catacgcaat
    16381 caagaacata ataccgacga tcaagccata tcgtaattta gaatagcgaa tttctttgat
    16441 agctagaaac atttgttttt cctccctatg atagattgat agtgacaaaa tgtcactcgt
    16501 tgaatagtgt aactccaaag tgacattttg tcacttatgt tgtttttttt cttatcaata
    16561 aattgtctaa gacatctttc gataaaacct taacatgagt cataaaaaat ttatttaagc
    16621 cttatttatg ctttccatga taaactaaag tgaccaaatc aatgaggtgg gaaaatgatt
    16681 aaactattag tagtagaaga cgataaagta ttatccaata atataaaaga aatactcgct
    16741 gcaatcggcg agataacaca agtttatgat ggtagtgaag gactgttcga gttggaaagt
    16801 ggagtttatg atcttgcaat cttggatttg atgatgccgg aaatgaatgg atatgaagtg
    16861 ttagcaaaaa tacggaaaga aaaaatacag actccagtat tgattttgac tgcaaaagat
    16921 agtttggaag ataagattga aggatttgaa cgaggtgcag atgattatct aacaaaacct
    16981 ttctatcgag aagaactgat catgcgcgtg aaagcattat tgaaacgctc gctaggtctc
    17041 tttgatgaaa atgttctgaa gtataaggat atcagttgtg atttaaaaag caatgaagtc
    17101 ttgtataaag aacaagtttt gcccatacaa ggaaaagagt ttgaattgct cgtttatttt
    17161 ttacaaaata agggaatcat tttgacaaaa gagcagatat ttgaccggat ctggggattt
    17221 gattctgata cgacgattac agttattgaa gtttatatga gcaacttacg aaaacatctg
    17281 cgaagtactg gattggataa agaaatcgaa accttacgta atgtcggtta tatactgcaa
    17341 agggtgtgat gcaatgaatg gagatattac acgaaagcaa cgtagtcgct tttttgcgac
    17401 aaatgtagca gcatttgctt ttatcttttt tcttttggga ttcattactt tacagatatt
    17461 aaaccaatct gcatacagag aaacagatct gtcattagag aaaataaatt ttgattctcg
    17521 aacagtacaa ttagagatac aaagatatca gcaaggcgat ccctttttaa acccgactca
    17581 aacggaacaa aacagaacaa acatggaaca gccattaatg gaaccatcaa atgaccgttt
    17641 taacactcaa gtgatcttgt ggtctaaaaa tggtgagata ttgaataaag cagctattgg
    17701 tgggcgtttc aaccaaatca aagaattaaa aatggatacg aaaaatctga ataccatcca
    17761 aaatattgaa ttaaatgaag acagtggctc atctgccctc tctttccgtt cgattacgaa
    17821 aaaagcggag acgaatcaag atggcgttgc ctacatccaa attttagaaa atacaaatca
    17881 aatcaagaat tcattagcga cttttcgcac gattatgatt ttgtgtatga ttgttttttg
    17941 gattctctct attggtatca gttattactt gtctagtcta agcatgcgcc ctattattct
    18001 ttcttggaga agacaaaaag aatttgttga gaatgcttct catgaattgc gcacaccgtt
    18061 aacgattatt caaaatagtt tggaacatct ttttaccaag cctaatcata ctattattga
    18121 agaatccgaa agcatcgctc aagcattgtc agagacgagg cgcctcactg gattgacttc
    18181 ggatttgctg acaatcgctc ggaatgattc tgatcagcag ctgctttcaa agcaaatgat
    18241 caatacacaa gaatatatcg aacaacttgt taaaccattt caagagatgg ccaccataga
    18301 tggaaaagag tttattctcc ataacgatgc aacattagaa gtaccaattg acgaaaaaaa
    18361 aatccatcaa gttttggtta ttttgctgga taatgcatta aaatatacga atccagaaga
    18421 taagatcatt ttggactcat ctgttaacca taaagaatgg ctgattcacg taaagaatac
    18481 tggcccttct atcagtgaag aagatcaagc aaggattttt gatcgatttt atcgggaaga
    18541 tcaatctagg gcgaaagaga ctggcggata cgggcttggc ttggcaattg caaaacaggt
    18601 aatcgaacag catcatggac aaatttctgt atccaattat cttccacaag gagtcgattt
    18661 taaagtccaa ttgccgttga agcctaaaaa ataatgctta tccctaaaaa agggctgtga
    18721 caaatgtacc gacccccaaa agttagacca aaaaatctaa cttttggagg tcggtatttt
    18781 ttatggctaa gtatactttt gaacttaaat taaagatcgt tcatgattac ttggatggta
    18841 aaggcggaag tgattatctg gcaaagaaat attccattaa ggctccatca caagtgaagc
    18901 gctggatcaa tgcctatcaa gaatttggtg aagagggtct agtaagaaag cgacaaaaga
    18961 aaaaatattc tgttcaattt aagctggatg cgatagagtt atatctaaca agtgagctgt
    19021 cctatcgtga agttgccaac acacttaaca tgaacaaccc taatttgatc gctagttgga
    19081 tgcgtcaatt ccgtgagggt gggatcgatg gactctcaaa aacgaaagga tgtccaccta
    19141 tcttgtctaa gaaaaatgaa cccaaaaata agaaaaaatc tacaactaaa gcaacgtcta
    19201 aagaacgtga acgcatcgag gagttggaga aacgagtccg ttcccttcaa attgaaaatg
    19261 cctttttaaa agaattgagg aagctgcgaa aacaggaagc ccaacaacga cgaatgaagc
    19321 aatcgcacga atcatcgcaa gcctccgagg accgtttaaa ttagttgagc ttctagaaac
    19381 tttaaagttc cctaaggcaa cctttatgta ctggcaaaaa cgattggacc gtaaaaatcc
    19441 agatcaaggg attgaagcgg aaatgcttaa agttcgggag aagcacaagg attatggctg
    19501 tttacggatg accaatgaac taagaaaccg cggtttctct atcaataaaa agaaagttca
    19561 gcgcctgatc aagaagctag gaataaaagt aacgacatac acacgtaaat cacggcgcta
    19621 caattcttat cgaggacaag t
//
LOCUS       DAGCYL010000036        18915 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.037, whole genome shotgun sequence.
ACCESSION   DAGCYL010000036 DAGCYL010000000
VERSION     DAGCYL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.037"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..216)
                     /locus_tag="MB216_000990"
     CDS             complement(<1..216)
                     /locus_tag="MB216_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBT4525843.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENN"
     gene            complement(213..731)
                     /gene="msrA"
                     /locus_tag="MB216_000991"
     CDS             complement(213..731)
                     /gene="msrA"
                     /locus_tag="MB216_000991"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBT4525844.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(751..1464)
                     /locus_tag="MB216_000992"
     CDS             complement(751..1464)
                     /locus_tag="MB216_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBT4525845.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(1467..2363)
                     /locus_tag="MB216_000993"
     CDS             complement(1467..2363)
                     /locus_tag="MB216_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBT4525846.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(2521..3363)
                     /locus_tag="MB216_000994"
     CDS             complement(2521..3363)
                     /locus_tag="MB216_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBT4525847.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3495..4148
                     /locus_tag="MB216_000995"
     CDS             3495..4148
                     /locus_tag="MB216_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBT4525848.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(4211..4738)
                     /locus_tag="MB216_000996"
     CDS             complement(4211..4738)
                     /locus_tag="MB216_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBT4525849.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4758..5705)
                     /locus_tag="MB216_000997"
     CDS             complement(4758..5705)
                     /locus_tag="MB216_000997"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBT4525850.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(5717..7603)
                     /locus_tag="MB216_000998"
     CDS             complement(5717..7603)
                     /locus_tag="MB216_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBT4525851.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(7763..9841)
                     /locus_tag="MB216_000999"
     CDS             complement(7763..9841)
                     /locus_tag="MB216_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBT4525852.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(9854..10129)
                     /locus_tag="MB216_001000"
     CDS             complement(9854..10129)
                     /locus_tag="MB216_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525853.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(10491..10946)
                     /locus_tag="MB216_001001"
     CDS             complement(10491..10946)
                     /locus_tag="MB216_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525854.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(11100..12308)
                     /locus_tag="MB216_001002"
     CDS             complement(11100..12308)
                     /locus_tag="MB216_001002"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBT4525855.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(12420..12752)
                     /locus_tag="MB216_001003"
     CDS             complement(12420..12752)
                     /locus_tag="MB216_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBT4525856.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            12900..13775
                     /locus_tag="MB216_001004"
     CDS             12900..13775
                     /locus_tag="MB216_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4525857.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(13857..14438)
                     /locus_tag="MB216_001005"
     CDS             complement(13857..14438)
                     /locus_tag="MB216_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBT4525858.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(14444..15703)
                     /locus_tag="MB216_001006"
     CDS             complement(14444..15703)
                     /locus_tag="MB216_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBT4525859.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(15705..16751)
                     /locus_tag="MB216_001007"
     CDS             complement(15705..16751)
                     /locus_tag="MB216_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525860.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(16986..17261)
                     /locus_tag="MB216_001008"
     CDS             complement(16986..17261)
                     /locus_tag="MB216_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBT4525861.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(17504..18814)
                     /gene="der"
                     /locus_tag="MB216_001009"
     CDS             complement(17504..18814)
                     /gene="der"
                     /locus_tag="MB216_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBT4525862.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
BASE COUNT         5249 a         3941 c         3218 g         6507 t
ORIGIN      
        1 attattttct aaatattttt cccatgcttc gtcaaagata tccatggacg gcaaatagtc
       61 gacattcagt tccagataat tagaaatttc ttcgtagcta gagctgtgct taggaaattg
      121 gatgtcttta ccggcttctg tagcgaaaac agataaagca tcatgtgtgc caccgcctcg
      181 cagtgtcatt aaataatgaa aaaagcttcg attcaccctt cgttcctcca attttcttcg
      241 ataaaggcag cccggcgtgc atggtttctt ttaaaatttt caggatgctt tttataatac
      301 tcttgatggt cttcttctgc tagataaaat ggttgggctg gttcgatttt tgtaacgatt
      361 ggctgttcga aacgtccact ttcagctaac tgtttcttac ttttttcagc aatgatccgc
      421 tgttcttctg tcaaataaaa gatcaccggg cgataattgt caccgcgatc ctcgaattgt
      481 ccgaaagcgt cagttggatc agtttgctgc cagtaaagtt ctactaattg ttcatatgaa
      541 atcaatgttg gatcatagat aatctccact gcttctgtat gtccagtcgt ttggcttttt
      601 acttgttcat aagtcggatc tggtacatgc cctccagtat atcctgacat cactgtatgg
      661 ataccaggaa gcgtgtcgaa aggctggatc atacaccaga agcagcctcc tgcaaatatt
      721 gctcgttcca ttatgattcc ccctctaata ctactcatcc gatgattttt tattttcttt
      781 tgtcttgctt tcagtactgc tggtgctagt tttgttggta tttgagttgt ttgaagcatc
      841 agaggtatct tttggcaaat atagactaaa ccgaatgtca tcatttacta agtcgatttt
      901 atctgcttta atatacatcc cgttttgcat tcggaattga tccaacctaa gcatgatagt
      961 cagatcatct gtattgactt cgatccaatc tggtaattta tagtttctct tgatcatatt
     1021 catcacatct ttgattggca aactcaatgt tccgatagaa agacttctag cttttagttg
     1081 gacatttccg ttttccatca catacggatc aaaatataaa tagaaatcga taggaaaacc
     1141 tagtacttcg aaagtaccat tgagtagtgc ttcattttcc aaataaaatt tatattcaat
     1201 atcactatct ttttgatact cactaaggaa aaagctgatg atttgattga ctttttcttt
     1261 atttgaattg atcgttaaga caggatctcc ttgtctttcc gtaagagctg gtatttctgg
     1321 gagttctggt tcccgattag caaaaatacg gctgcctaaa aatacggtcg aaccaagtac
     1381 taccccaaga aggataagga aggcgaattt ccagtaattt gttttctttg ttttgtttga
     1441 agatacgtca gatcttgttt tcatgtttat tgatcatcct ccttctctaa ccattggttt
     1501 tgtgtctcga tcagctgatc tctcactgca tttgccatca attgataacc taaattattt
     1561 ggatggaaat gatcttcttc atagagcaca ttatttttga tgccatttag attttctgtc
     1621 gtgctagttg tttgatctgt tccttcttct gtgacaccta cttcctgatt caatccttga
     1681 taaagcaaat cattgattgg gataaagtag cagttcggca ttttttttgt caccgtttct
     1741 gttcctttgt tccaattgtt aacaattgtc tgcatgtcag tgatctcagg aaagttcaaa
     1801 taaaatggat tgtagatgcc taaaatgtaa ataggtgcgg ttttattgag ggatcgaaat
     1861 tcttccaata gttctgtgac attttcctga tagtttttca gtggcttttt aaaggaattg
     1921 accgttaacc cgaataaatt attttggata actttcatca aatcattacc gccaacagtc
     1981 agtgtgataa aatcagctgt tttgatattg tttcgaattt cttcttttgt ttttactcgc
     2041 tttaagatct ggtcacttcg ttcaccattt acaccatagt tttctgtttc aaccgcggtc
     2101 aaattataac ggtcttttag atcatttgcg acaaggggaa cgaatcctcc ttgcttggtt
     2161 tgatctccta ctccttcagt cagcgaatct ccgatagcta tgtagtgaag tttttctttt
     2221 ttatttggat cttctctcac agtagttact ggtttcatta gtgggccagc tttcggtata
     2281 gaagcagcta aacctgcata gatgatgatc gcaattaata atgggaacaa aacgaggatc
     2341 aaatgtcgtt tcgttaattt cattttttca cctcttgtat tcgattggtt ctaatgttat
     2401 caaaaatttt atcaaaaagg tagtctttat gaaaaaagaa aacaattatt ttcctaattt
     2461 tataaagaaa aagagaataa gctgcaagcc tttcaggctg agggcttatc ctcttttcga
     2521 ctaatctaca taatacatga ttgcaaacgc gtctttacca gtatgcgtag cgataaccgg
     2581 attcgtatgc agtacaggga tatgcatgtc cgggaataat tcttgcaaac cctctttgaa
     2641 ttcttgcgcc aattcttttg catccgcatg tgaaataccg atttgtcgca catttggcaa
     2701 ttctttcagt tcatttttca aattattaaa ccatttatcg aacgttttct ttcctcgccc
     2761 cttagttaca gggattaatt cagaatgggc gaaattcatc accacgcgta tgttcaaaac
     2821 actagacagc aaaccagttg ttcggctgat tcttccacct tttaccaagt tatctagtgt
     2881 ggatattccg atgtataatt ttgtattgtc ttgtactttt ttgacttctt caaggatttt
     2941 ctctactgtt ttaccttctt ttgctaattc agctgctcga attacctgaa aagataatcc
     3001 ttggtcagtg gtatcactgt cgataaccgt tactttcgtt tttgttagat tactggcttg
     3061 tcttgctgct tctactgttc cactcaagcc ttttgtcata tggatcgaaa tcacttcact
     3121 gccgtcttct cctagctcat catacatcga gatgaattct ccaattggtg gttggcttgt
     3181 tttgggcaga ttttttgctt tcgccatcat ttccataaat ctttcgccgt ctagttggtc
     3241 atcatcggga tatactgttc cgtcgatcat gatggatagc ggcatgacac gaatgcctaa
     3301 agcatcgcgt acattttttt ccatcgtaca tgaagaatct gttacgattt ttacatttgt
     3361 cattattttc tctctctctt tctttaacaa gatgccttat tcatgttaaa ctacatgtaa
     3421 gacaatttct atttttagta taacagacaa ataatgattt ttcattaatt acacctagta
     3481 aaggagccgt tttcatggaa aaaacgaaat ttaatcgaac gtatgaaata ttaaatgaag
     3541 tgtttaatgc agtcacccat ggaattggga ctggattgag tatcgcggga ctagtggttc
     3601 ttttagtaaa aggtgcccac ttgcattcct ccctccatat tgtctcttat gctatctatg
     3661 gatcgatgat gattctttta tttttaacgt ctactttgtt tcattcattg atttttacaa
     3721 aagcaaaaaa ggtgttccaa gtattcgatc atagttcgat ttttcttttg attgcaggaa
     3781 gttatactcc tttttgtttg ctgagtatcc gaggatggct aggttggact ttatttgtat
     3841 taatctggtt gtttgcgatt cttggcatta tatataaagc gttgacgttg cataaacggg
     3901 agagagtatc aaaagtatct acgatcatct atatttttat ggggtggctc tgtgtcgtgg
     3961 ctgctgttcc tctttatcac tctcttggaa tgactggtgt tattttgatg gtagcaggcg
     4021 ggctttctta cacgataggc gcattctttt atagcttgaa ttctatccgc tatatgcatg
     4081 ttgtttggca tttgtttgtt atgttaggtg caggttttat gtacttctct gttttattct
     4141 ttacttaata aaaaaaataa agctatggat gggacagctc taaataacag ctagtctatc
     4201 catagctttt ttatatcgtc aattcgcctg tcccgctttt tacaggattc tcattacgct
     4261 gatacgtttc aaattgatgc ggataacgat ttttttcatc catttctcct tgactagtat
     4321 tgatcagtga ccaatcagac cagtcaacag gtggaaaata agcatctcca tcgaatacat
     4381 gatggatgac tgtacggtat aatatcgtac aaaatggcag aaattcttga taaacagctg
     4441 accctccagc gatgaaaaag ataccgtctg tttctttttc cagagcaagc acgtcttcca
     4501 cactatgcat gacatgcacg ccttctgctt tataggaaga atctctcgtc aaaacaatcg
     4561 tctgacgatt cggcaacgga cggctaccca tcccttcgaa tgtttttcgt cccatcacta
     4621 gtgtatttgc ttctgtcatt tgtttgaaga atttcagatc atttggcaaa cgccaaggaa
     4681 gacggtcttc ttttccaatc aatccgtttt catcctgtgc ccaaattgca gctaacattt
     4741 tctcatcctt tctgattcta aacagcgata ggagctttga tcgccggatg tgggtcatat
     4801 ccttctaatt ggatatcttc catctcaaaa tcaaatactg actgcttttc ctgattcaaa
     4861 actaaagtcg gaaatgcgcg tatttctcgg ctcaattgtt ctttcatttg ttcgatatga
     4921 ttgatataaa gatgagcatc tcctaatgtg tgaacaaatt cgccaaccgc taaacctgtt
     4981 tcatgtgcaa ttaggtgagt cagcaacgca taacttgcaa tgttgaacgg aacgcctaga
     5041 aacacatccc cactgcgttg atacaattgg cagcttagcc gtccctcttg aacataaaat
     5101 tgaaacatcg tatgacaagg aggcaaagcc atgctaggaa catcttccgg attccatgca
     5161 gaaacgatca atcttctaga atcaggagtc tttttgattg cttcgatcac ttgtttgatt
     5221 tgatcgataa agccaccgtc ttttgtttcc cagtgacgcc attgatagcc gtagatgtgt
     5281 ccaagatcgc cgtatgtttc agcaaaggaa tcatcggtta ggatcttctc gcaaaaaagc
     5341 ttgctctgct ttttatattc ttcattgaat tcaggatctg tcaaacatct atggccaaaa
     5401 tcagtcatat ctggtccatt atagtctgga cttttgatgt agcgttcaaa tgcccattca
     5461 tcccaaatat ggtttttatt ttgtaataga tagcggatat tcgtatctcc ttttaaaaac
     5521 caaagcaatt cgcttttgat caatccaaaa ggtactcgtt tcgttgttaa caaaggaaac
     5581 cctttttgca gatcaaaccg catttggtat ccgaataagc ttaatgtgcc tgtgcctgtt
     5641 ctgtcttctt tacgatgccc ttcttccaac aattttcttc ctaagtcaag atacgcttgt
     5701 tccatccttt ttcctcctaa tcttctaccc attcactcaa gtattcccac cgtgccattt
     5761 tttcttcaag ttgtgcttct gtttcagata cgtcgttttg caattcttgt aatcgagtga
     5821 aattatctcc ttgatgattc atctcttctt ggagcaatga gattttttct tcaagttctg
     5881 caatctcatc ttcaatcgtt tcccattctt tttgttccat gtaagataat ttcttctttt
     5941 cagcaggttc cttgctctgt atttttggtt tcgctttttc agcaggctgc tgggtctctt
     6001 tttgcttcag taaataactg gacatgtttc cgtaataaag ttcttgtttt ccgtttcctt
     6061 ggaatactaa taacttatct gctaccttgt ccaagaaata acggtcatgg gaaacagtga
     6121 tcacagcacc tggaaaactt ctaaaataat cttccaaaat cgtcaatgtt gctatgtcca
     6181 aatcattggt cggttcatcc aataataaaa cattaggttg ctgaatcagc agtttcaaca
     6241 aaaataaccg gcgtttttct ccgcctgaaa gtttgcctat cagtgtccca tgcataaatc
     6301 ttggaaataa aaatcgttca agtaattctg ctactccgat ttgactgcca tctgctgttt
     6361 ttgcttcttc agcagcttct tgtaagtaag caatcatccg tttatctggt gccatttctt
     6421 cattttcttg tgtataatac gcaagatgaa ccgtttcacc gatagacatt gtaccgctat
     6481 ccaggggaat tctgccagct agaatattaa gcaatgtcga ttttcccgca ccatttttac
     6541 cagtaatacc tagtctttcc cgagattgaa tcaataaatc taaatgttct agcagtgttt
     6601 ggtcatttat acggtaactg ccatctttga tttccagtac ttttttccct aaacgttgtg
     6661 tcgctaggtt cagctctaaa tcatcttctt ggtttacttg gtacaagttc tcctttagat
     6721 cttcgaaacg gttgatccgt gcttgctgct tggtgccacg tgcctgtacg ccagccctca
     6781 tccaagctaa ttcctgttta taaagccgct tgcgcttttc ttcttgttct acttctacac
     6841 gatcacgttc tgctttttct aagacgtaag cttcataatt tcctttatac tcgtataatt
     6901 ttccaaatga aagttcgaag attcggtttg ctactctatc tagaaaataa cgatcgtggg
     6961 taaccatcaa tagtgcgcca cggtattgtt tcaaatagtt ttccaaccac tcgatcgctt
     7021 cataatctaa atggtttgtt ggttcgtcta gtaatagtaa atcgggttca tcaatcaaca
     7081 cttgcgccag actaactcgt tttttctgac cacctgataa agtactgatt tttttatcta
     7141 attcactgat tcctaatttt tgcaaaataa tctttgcgtt ggtatctgta gtccaagcat
     7201 cttctttatt catttgttct tctgctttag cataacgttt ttgcacatca gggtcttctc
     7261 cgttttcccc tagttcgatc aatgcttcct catatgctcg aacagtttgg atcaaggggc
     7321 tgtttccttg aaagactgct tggagaacag ttaattcatc ggaaaattca gctgcttgag
     7381 aaagataacc aatacggtaa tcagttggat aaaacactgt ttgccgatca ccgtcgctgc
     7441 tatcgatacc ggcaataata tttaagagac ttgttttacc cgtcccgttc gtcccaatca
     7501 acccgatacg gtctttttcg tggaccaaaa aagaaatctg gtcaaacaaa tctttttcac
     7561 cataagtttt tttgaggtca gttactttta attctttcaa catgttccct ccttatcttg
     7621 gatattgtat cataaaaata gtgaacttca ggaaaatttt tttggtattt gttctttttg
     7681 tgttctcaaa aaaagaacag gaccgattcg atcctgttct gatatgtttt gttttttata
     7741 aaacttgaaa atttttacct ttttatttta ttggactcgt tttttttgac tttgctcgaa
     7801 acgcaaatcg gaaagaaaag ttatcagatg gtttgttcat cgtataagat tctaacaaat
     7861 tgatattcct gcgatagctg ttcgcagaaa acgtcgtgat tttttcagca acttcgtatt
     7921 catcaatgat ttttctttca agataataac ctcttagcat ttttttcgta aacagactat
     7981 cttgctggat catccacgaa gaaatcaaat tccccgagtt catttttatt ccttcaagtt
     8041 tgcgttgctg gataaaaaat tgaaccatcg tttcatcata aatatcagtc aagttatcta
     8101 aactttcaat aatcaactga gtattttttt gatatgtctc tctgatttct tggatatctt
     8161 catcactaat aaatgtctga cgatcagcat atcttctttg ccaaaacatt ttcggatgga
     8221 gaacgattcg cattaaccta cggacaacaa tgaaccaaaa gccgataaaa gacaaaatct
     8281 ggcttgtgat agaatgttcg aatcgagaaa gaaaacggga gtaaaaacga taaccattag
     8341 aagaaagctt gcccgaacga taactttcat ccaatccatc ccgttcgatc gacaaaatca
     8401 gtgcttgtat ttcttgtact tcttgctttt catcatcggt taaatcttcc aagtacagat
     8461 cacgtatccg ttcttgataa ttttctatca ctgcttcagt ggctaaaaat tcttttgtat
     8521 tacgatcaat ttcctttatt tcttttcgca aaacttcaac gacctcttct aaaatcatca
     8581 aagcattcaa atccgtgctt ttcgcttcct ctgtttccgt cagcataggc aagacaatga
     8641 tccctatgac caaagtcact aagatgacac ccgccgtcag aaacaaaagc aaagaccgct
     8701 ggtaaaacat catattattg attgaaaatg gcaaaataaa aatcgtagcc aaactgactg
     8761 ttccctttac accgccaaat gttaagatca agatctcgtt caactgtttt ttgaactttt
     8821 tactgccatc tttgaataca taaaaaagac tgatagaaat gaatctgatc acaaaaagca
     8881 tgacagagat taatacgata atcgctaata aaagaccgtt tggatataaa ccgtctcccc
     8941 atactggcga gaacacttgc gtcaattcga ttcctaaaaa tataaatact aatgcattca
     9001 gtgtaaaagc aatagttgtc catgtactgt gggacaagct cgacaattct gcatcaaaca
     9061 cagagatttt cctaaaacca gaagcttgga gaatacctgc tgccactgca gcaatgattc
     9121 cagatacatc aaatgcctct gccaatacat aggctagaaa aggaagcaaa agttcgatca
     9181 gcagataccc tgtgacatct tgtgctgatg ctttttcgat caaatgaatt attttttgtt
     9241 tgaaccagac aagcaaaaag ccgatcaaag ctcccccaat actagaaact actagactca
     9301 tccccgcgtt tactgctgaa aaagatccgg tgatcagcgc accaagagca aactggaagg
     9361 cagtaacacc agatgcatca ttcaaaagac cttctccttc caaaatatgc atcgcctttt
     9421 tagggatatt cactcgtcca gaaagagaac tgactgctac cgcatctgtc ggacctaacg
     9481 ctgctccaaa agcaaagcag gcagcaagtg ggacacttgg caacaggaat gataacgtcg
     9541 cacctacccc gacaagggtc aaaatgactc ctccaaatgc caagaacaaa attgtcccga
     9601 aatttttgag aatcgaagaa atatctgctt tttctccttc tctaaataac aatggtgcaa
     9661 taatcataac taagaacaat tcaggttcaa aatcgattga ttgtccccac tctgtcagac
     9721 ctaataccac tccaaggaaa atttggatca gtggagctgg aatcattggc aaaatgcggc
     9781 tagctatgtt cgataaggtg atcgttactg caaatacaat aactaactca gcaaattcca
     9841 tcgtccatcc cctttatttt ttattttgtg taagcatttt taaaaattgt tcttttttct
     9901 ttaaaatttt ggtttcgact acttcaacct gtgtttggtt cccctctaat tcacactgta
     9961 cacgttttga ctccaaacga agcagatcat gtttcagacg ttccaactga cgatacatca
    10021 atatatttct tttacgcaac ttacaaagta tttgacggcg gtcatccatc gaaagggcgc
    10081 catagcgatc atgcaacagt tttttttgtt gttcaaaact ctctctcatt ttccagatct
    10141 cctatacaca aacttccatc attatagcaa agttcttttt ggatgaaaaa tatttcattg
    10201 tttcatcgtt tattgctagt tagaaatttc cataaatttt tcaacgcctc attctattcc
    10261 aaatagtatc agatatcaag taaaacttaa gaaagataaa agtttctgac aaaaggatgt
    10321 ttactttccc attcctgcct ttcttgttct tttatcacaa caaaaaaatg gggctgcgac
    10381 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
    10441 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
    10501 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
    10561 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
    10621 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
    10681 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
    10741 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
    10801 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
    10861 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
    10921 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
    10981 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
    11041 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
    11101 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
    11161 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
    11221 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
    11281 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
    11341 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
    11401 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
    11461 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
    11521 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
    11581 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
    11641 gtttcaataa atgcatggat tcccgcggcc cgttcatttc ccaagagcat cttgatgaat
    11701 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
    11761 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
    11821 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
    11881 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
    11941 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
    12001 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
    12061 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
    12121 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
    12181 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
    12241 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
    12301 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
    12361 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
    12421 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
    12481 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
    12541 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
    12601 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
    12661 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
    12721 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
    12781 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
    12841 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
    12901 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
    12961 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
    13021 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
    13081 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
    13141 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
    13201 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
    13261 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
    13321 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
    13381 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
    13441 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
    13501 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
    13561 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
    13621 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
    13681 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
    13741 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
    13801 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
    13861 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
    13921 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
    13981 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
    14041 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
    14101 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
    14161 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
    14221 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
    14281 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
    14341 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
    14401 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
    14461 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
    14521 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
    14581 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
    14641 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
    14701 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
    14761 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
    14821 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
    14881 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
    14941 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
    15001 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
    15061 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
    15121 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
    15181 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
    15241 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
    15301 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
    15361 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
    15421 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
    15481 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
    15541 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
    15601 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
    15661 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
    15721 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
    15781 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
    15841 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
    15901 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
    15961 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
    16021 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
    16081 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
    16141 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
    16201 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
    16261 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
    16321 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
    16381 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
    16441 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
    16501 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
    16561 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
    16621 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
    16681 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
    16741 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
    16801 tagaaaagat aagagcaaag ctcacctcat aacttgcaaa taaataaaaa ttaaataaaa
    16861 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
    16921 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
    16981 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
    17041 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
    17101 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
    17161 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
    17221 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
    17281 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
    17341 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
    17401 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
    17461 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
    17521 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
    17581 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
    17641 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
    17701 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
    17761 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
    17821 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
    17881 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
    17941 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
    18001 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
    18061 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
    18121 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
    18181 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
    18241 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
    18301 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
    18361 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
    18421 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
    18481 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
    18541 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
    18601 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
    18661 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
    18721 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
    18781 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
    18841 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
    18901 gtatttttat tcctt
//
LOCUS       DAGCYL010000037        18504 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.038, whole genome shotgun sequence.
ACCESSION   DAGCYL010000037 DAGCYL010000000
VERSION     DAGCYL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.038"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            99..746
                     /locus_tag="MB216_001010"
     CDS             99..746
                     /locus_tag="MB216_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBT4525863.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="MB216_001011"
     CDS             1139..3784
                     /locus_tag="MB216_001011"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBT4525864.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="MB216_001012"
     CDS             4094..5407
                     /locus_tag="MB216_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBT4525865.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="MB216_001013"
     CDS             5397..6050
                     /locus_tag="MB216_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4525866.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="MB216_001014"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="MB216_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBT4525867.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="MB216_001015"
     CDS             6947..7147
                     /locus_tag="MB216_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4525868.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="MB216_001016"
     CDS             complement(7401..9080)
                     /locus_tag="MB216_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBT4525869.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="MB216_001017"
     CDS             complement(9082..9294)
                     /locus_tag="MB216_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBT4525870.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="MB216_001018"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="MB216_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBT4525871.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="MB216_001019"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="MB216_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBT4525872.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="MB216_001020"
     CDS             13269..13466
                     /locus_tag="MB216_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525873.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="MB216_001021"
     CDS             13619..14104
                     /locus_tag="MB216_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBT4525874.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="MB216_001022"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="MB216_001022"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBT4525875.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="MB216_001023"
     CDS             14981..15508
                     /locus_tag="MB216_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525876.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="MB216_001024"
     CDS             15599..16336
                     /locus_tag="MB216_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBT4525877.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="MB216_001025"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="MB216_001025"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBT4525878.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="MB216_001026"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="MB216_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBT4525879.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5983 a         3201 c         4023 g         5297 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt atcttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acgaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DAGCYL010000038        18378 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.039, whole genome shotgun sequence.
ACCESSION   DAGCYL010000038 DAGCYL010000000
VERSION     DAGCYL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.039"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            450..872
                     /locus_tag="MB216_001027"
     CDS             450..872
                     /locus_tag="MB216_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBT4525880.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            869..1099
                     /locus_tag="MB216_001028"
     CDS             869..1099
                     /locus_tag="MB216_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4525881.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            1597..1797
                     /locus_tag="MB216_001029"
     CDS             1597..1797
                     /locus_tag="MB216_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBT4525882.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            1824..3017
                     /locus_tag="MB216_001030"
     CDS             1824..3017
                     /locus_tag="MB216_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4525883.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(3085..4650)
                     /gene="guaA"
                     /locus_tag="MB216_001031"
     CDS             complement(3085..4650)
                     /gene="guaA"
                     /locus_tag="MB216_001031"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBT4525884.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(4709..5632)
                     /locus_tag="MB216_001032"
     CDS             complement(4709..5632)
                     /locus_tag="MB216_001032"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBT4525885.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            5828..6433
                     /locus_tag="MB216_001033"
     CDS             5828..6433
                     /locus_tag="MB216_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4525886.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            6741..7757
                     /locus_tag="MB216_001034"
     CDS             6741..7757
                     /locus_tag="MB216_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBT4525887.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            7898..9199
                     /locus_tag="MB216_001035"
     CDS             7898..9199
                     /locus_tag="MB216_001035"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBT4525888.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            9245..9907
                     /gene="deoC"
                     /locus_tag="MB216_001036"
     CDS             9245..9907
                     /gene="deoC"
                     /locus_tag="MB216_001036"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBT4525889.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            9928..10326
                     /locus_tag="MB216_001037"
     CDS             9928..10326
                     /locus_tag="MB216_001037"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBT4525890.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            10392..11474
                     /locus_tag="MB216_001038"
     CDS             10392..11474
                     /locus_tag="MB216_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4525891.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            11686..13248
                     /locus_tag="MB216_001039"
     CDS             11686..13248
                     /locus_tag="MB216_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4525892.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            13241..14374
                     /locus_tag="MB216_001040"
     CDS             13241..14374
                     /locus_tag="MB216_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4525893.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            14374..15330
                     /locus_tag="MB216_001041"
     CDS             14374..15330
                     /locus_tag="MB216_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4525894.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            15429..16595
                     /gene="deoB"
                     /locus_tag="MB216_001042"
     CDS             15429..16595
                     /gene="deoB"
                     /locus_tag="MB216_001042"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBT4525895.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            16619..17437
                     /locus_tag="MB216_001043"
     CDS             16619..17437
                     /locus_tag="MB216_001043"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBT4525896.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            17454..18161
                     /gene="deoD"
                     /locus_tag="MB216_001044"
     CDS             17454..18161
                     /gene="deoD"
                     /locus_tag="MB216_001044"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBT4525897.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         5870 a         3332 c         4010 g         5166 t
ORIGIN      
        1 cctatagaac actgtggaat ttattaatgc gatgaatcca atttcaaata gaaaggtcgc
       61 tgattttatt tacctataca actttataaa atttagtcat actcctagaa agtagataaa
      121 attggcgact taatggaggg aaaagtcgcc atgagaaaga taatctgtca tctagttctc
      181 ttaattaagt tcttatcaaa aaaacttatc cacctgtact ggatagatat gaccatcaat
      241 gtggttttgc agattacata gatagaaaaa tcaaagcata tgaacagaga aaacaatctg
      301 tttatgtgct tttttggtca cttaataaat atcttttagg atttctcttc atttatgcaa
      361 caaatccccc gttcgtgtcg gggtttagta tgaagggaga taaagaagca catgccactc
      421 cctttcacga cagaaagggg gtgaaaagca tgaaaccatc ttcttttcaa gaaaccatag
      481 aaaatcagtt tgactatatc tgcaaacaag ttattgaaga tgaacgaaaa gattacttca
      541 aacatctaga cagactatca gcaaaagaag tctcgttttc tgatatagat aactaccttg
      601 tcaactcttt ttctactgtt gatacgtacg tcactgattt ccaactattt aaattatttg
      661 gattaagaat caatgttgaa agtgatttat taagtgaagc cctgcgtcat ctttcagaga
      721 aaaaacggaa cattatttta cttcattact ttatggatat gagtgatgtg gagattgcgg
      781 agttactaaa tttgaatcgt tcgactgttt atcgtcatcg aataagtgga ctagccatga
      841 ttaaagaatt catgaaggag tgtaaagaat gaaaacagct tatccaaggg ttcccttccc
      901 cctcattgta aaggcgacag atggcgacgt ggaagcaatt aatcagattg ttaaacatta
      961 tcgagggtat acctcaaaac gttcattacg tcgcatgaca gacgaatatg gaaattctca
     1021 tatggttatt gatgaaaccc tacgtggcag aatggaaacg aaactcatct ctaagatttt
     1081 atcgttcgaa attaaatagt cccatttttt cgtggaagcg tgtcgaatcg tatccacgct
     1141 tcccgatttg ggaggtttgt tattccatca aagcatattg tgtgttcagt aggttttgat
     1201 aggctaacaa gccaaacgtt ctttgacaac caaataaagg caatcgaata cgtttcggta
     1261 agaaaagagc tgatgaacta acacgccatg acctacaaaa ttagtgagcg attcatgtta
     1321 gtaattcgaa agtaatcttt agcaggattg ctgccatgac tttcttatcg tgataatgat
     1381 actcccgtat agccatagtc cgagcgttag aagcgtcaca ggcaatgggt acggctacat
     1441 gagaaccatg cagaagtgaa attcctgtga gctttgctag agctgttcga ttgctaagat
     1501 attcttctct tgtcagtgta ttattataaa actatttgat ataattatag gtgaatattg
     1561 gagttttcct agcctataat tgggaaagga gtaataatga taacggtcac aaaaaaggat
     1621 ttagttgagc taggatatgg aacttctttt tcagcagaca tcataaaaaa agcaaaagaa
     1681 ttgatgatta caaaagggca tatctactat caatctcgaa agctagatag agtccctcgt
     1741 gaagctgttg aagaaattct aggaattcgg ttttcaaatg aaaacaggtc tgagtagttt
     1801 ttgcacagta aggagtaaaa actatggtta aagacccaat aaaaaaagca aagaatggaa
     1861 cctattattt tcgtgcaaat ctaggttatg attctaaagg gaaaaaaatt caaaaatatc
     1921 gtagtggatt cactactaaa aaagaagcaa gagaagaata ttcgaaactg ctattgatga
     1981 aaccagaaga attaagtgag aataaggata aaatgacgtt taaacattat atctttgaaa
     2041 ttttccttcc atggtataag acacaggtaa agctcagaac atatgaaaat cgcttaccaa
     2101 ctattaagaa gcatttctct tattttgata aaatggctgt ttccgatatt gagcctattg
     2161 atgtgcaaaa ttggcaacta aaattatcaa agaagtgtaa gtcttcttat gtaagagctg
     2221 ttcaaggact attttcagtt gctatggatc gagcaattgt ccttgggata accaccacaa
     2281 atccatctaa gattattggc aatgtgaaaa agcaaaagtc taaaattgaa ttttggacaa
     2341 aagaagaatt tgaaaaagta atctctctaa tttacaaaga agattactat caacactttt
     2401 tatttatctc tctgtggttt ttatttatga ctggaatgcg aattggtgaa gctactgcca
     2461 ttcaatggga agatattgat tttgactcag gagtactaac catcaacaaa acactctatt
     2521 acaagaatca atcgaattat agatttgttg aaccaaaaac aaaggcgagc gttcgtcata
     2581 ttgtattaga taaatgcaca ttaacttatt tatccgaatg gaaggaagta caacaaaatc
     2641 taattcagac agactttgta atgagctata acggtattcc tacccaaaaa catacactat
     2701 catatgctat tgaacgctat gctaagctgg ctggtatcca tcgaatcaga atccatgggt
     2761 tacggcattc tcatgcttcc ttgttaatca gtatgggaga aaatccactt atcattaaag
     2821 atcgtctagg acacgaagat attgagacga ctttaggaac gtatggacat ctttatccta
     2881 acagtaattt cgaggtagct aacaaattaa atggagccat ttcctttaaa gaagcaaaac
     2941 aaaattatga tacttcacca aagaatcaat ttactgtaga ctacttgcga aacaaagaaa
     3001 tgaaaaaagt gcaataaaag tgcagtaaaa agataagaag agccagaaac ccttggtata
     3061 agaggatttc tagctctcct acaactattc ccactccact gttgctggtg gtttagaagt
     3121 gatgtcatac acgatacggt tgacgtgggc tacttcgttt acgatacgta cagagatttt
     3181 ttgcaagact tcccatggga tacgggcaaa gtcagcggtc attccgtcga ttgaagttac
     3241 agcacggata ccgactgtgt agtcgtaagt acgtccgtca cccataacac cgacagagcg
     3301 gatccctgga agaacagtga aatattgcca gatatcacgg tcaagtccag cagctgcgat
     3361 ttcttcacgc aagatagcat cagattcgcg tacgatttct agcttgtctt cagtgatttc
     3421 gccaagaaca cgaataccaa gaccaggacc agggaatggc tgacgccaaa cgattgaatc
     3481 aggcatgcct aactgagtcc ctaattcacg gacttcgtct ttgaataatg tgtttaatgg
     3541 ttcgatcaat tcaaattgca tatcttctgg cagtccacct acattatggt gagatttgat
     3601 cgtctgtgct gtttctgtac cagattcgat aacgtcagta tacaacgttc cttgtgctag
     3661 gaaagagacg ccttcttcac cagcaagttt tgttgcttcg tcatcaaaaa catatacgaa
     3721 ttcattccca atgattttac gtttttgttc tgggtcagat acacctttca acttgtctaa
     3781 gaagcgatct cttgcatcga ctttgatgat gttcaagccg aattttccgc ctagactttc
     3841 cattacttgt tccgcttcgc ctttgcgtag cagaccgtgg tcaacaaaga tagatgtcaa
     3901 ttggtcaccg atcgcttttt gtaaaagaac cccaacgaca ctagaatcaa cgccacctga
     3961 aagaccaagc aagacttttt tgtcaccaac ttgttcacgg attttttcta cttgcatatc
     4021 gataaagtta tccattgtcc agttaccttc acagtgacaa acatcaaagg caaagtggcg
     4081 cagcaattcg attccgtatt cggaatgacg tacttctgca tggaattgga taccataaaa
     4141 attacgtgtt gtgtcttgga tagaagcaat cggacaatct ttgcttgttc cgactgtttc
     4201 aaagccttca ggaactttag ttaccaagtc accatggctc atccatactg tttgttgttt
     4261 tggtgtttct ttaaacaaga cagcttgatc gcttgttact tctaacaagg ctttcccata
     4321 ttcgcggttt ttagcaggct ctacggaacc gccaagtttg tacgtgatca attgcatacc
     4381 atagcaaatc cctaatacag gaatacctaa attgaagatc tcaggatcga tcccaaatgc
     4441 attctcatca tatacactgt ttggtccgcc agaaagaatg attccctttg gattcatcgc
     4501 ttttacttct tctgcagtaa tgcggtggct caataattcg gaaaagactc caagatcacg
     4561 aatacgtctt gtgatcaatt ggttatactg actaccgtag tctaatacga taatcttttc
     4621 aacgttcgtc atatcggcaa cgtttgtcaa atttattacc cctatccctt taaattttat
     4681 ctcaaaatag tcaaactatt ttaaaatgct aatattttcg catgaaaatc gaatcgatga
     4741 tatgattttc agttttatgg agaattatat cggctcttcc tcgtgtaggc aagatatatt
     4801 cctgtaaatt tttcaaattc acatttttcc atacatcttt tgccatttta aaggcttctt
     4861 cacgatctcc gatagcaaaa cgataataat aattctctgg attttggaaa gccgtatcca
     4921 gtaacgcacc aaaacgatcc aaataccatt tttcgatcag cacgggatcg gcatcgacat
     4981 agacagaaaa atcaaaaaag tcgctgacgt agatttgctg attcgcaggt aattgaagcg
     5041 tattgatccc ctcaacgatc aagatatcag gctgctggat caattcgtac ttgtctggaa
     5101 tgatatcata cacatcatgt gagtaaacag gtacttttat ctcttcctga ccagatttta
     5161 cacggttcaa gaaatcgatc agcatctcca tatcataact ttcaggaaat cctttacggt
     5221 ccatgattcc acgctctttt aatatacgat ttggataaag aaatccgtca gtcgtgatca
     5281 gctgaacgtt tcttcgtttg aaggtacgag acaaaatcgt ctgcagcagt ctggctgttg
     5341 tactttttcc aacggctaca cttccggcga tcccgataat aaaaggcggt acttctacgt
     5401 atttatgaag aaataaacct ttactcaaga acaaagattc aaattctttc atataaagat
     5461 aaatcagatg agcaagaggt acatagatat ctcgaacatc ctgcatggaa atgcggtcat
     5521 tcaaactttt gatattatct agttcatctt gtgtcaaagg tgcttgccca ttacgataaa
     5581 attctcgcca ttcttctctt gccacacggt agtagttcat tggatcattc atattagcct
     5641 cctgcgttct cccatcaatg gaactatttt aacacaaaat aaacgcaaac cccaccactt
     5701 aagagagatt gccgatcttt ttgtataatc tttataatag aaagaaacgg agtcttattc
     5761 ttgacttgcg cacggataca taaaaggata gactgttgtc atgaaagaaa tgagaaggat
     5821 ggaagacatg acaaatcatt actattctga acgcccagat acggcgcacg attttgaaca
     5881 atgggctttt gagttaaaag gcagaacctt tcaatttgta acagattctg gtgtattttc
     5941 acgtgaaaca gtggattatg gttcacgtgt cctgattgat gctttcgatt gggaagagtt
     6001 acctgatgaa ggaaagatct tagatgtggg atgcgggtat ggaccgattg ggttagcttt
     6061 ggcatttgcg acacagcggt ttgttgaaat ggtcgacatt aatgaacgag cggtaagtct
     6121 agcgcaagga aacgcaaagc gtaaccaaat cgaacatgtt gatatccatc aatcgaatat
     6181 ctatgaagca gttcatgaag aaacctatgc agctatcgta agcaatccgc cgatccgtgc
     6241 tggaaaaaaa gttgtacatg aaattttgac aggcgcatat cctcggttga agaagggcgg
     6301 tacactgaca attgtgatcc agaagaaaca aggagcgccg agtgcgcaaa agaaaatgga
     6361 agaaacattt ggtaatgcag aaatcgtcac aaaagataaa ggatattata tcatcaaaag
     6421 tgtgaaagaa taactagctg aaaaatagtg gagcgacgaa agagtggaac tgggcgtgca
     6481 tgtacgggta cgtatcagtt ctttttttgt tgagaagttt ttaaaaacaa agaactattt
     6541 tttcttcaaa aagaaatagt aggaacagag tcgttgtttt ttacgataaa ataaataaga
     6601 tacagaactt gtatagaagg ctgccaagtc ggtagataaa ggaggttttt atcaaaaaac
     6661 atactttcct tttttctcta ttcttttctt gggggttttg gtacactgat agggaattta
     6721 gatagaaggg agccaatagg atgaagctga ccatcagaga tatcgcagaa atggcaggcg
     6781 tatcagtaac aactgtctca cagattttga ataacaaagg aagcagattt agtgataaga
     6841 caagaaaaaa agtgttgtcg atcgtgaatg aacatcatta taagcccgat tatttcgctt
     6901 caaatgtgat caatcgacat tcaaagacca tcggaatgat cgtccctgat gtcactgatt
     6961 tcttcttctc caaactgatc gaaggtgtag agagctattt gaatcctttg ggctatgtga
     7021 tcatgttatg caattctcgt catagccaag aaaatgagat caagtattta caagagcttt
     7081 cccaccgctc agtggacgga atcttgctgg ccacaccaaa tattttacct gagaaatatg
     7141 cattagacag cggtttttat caaaaaatgc caatcatttt aatcgatcgc ggattgaatc
     7201 gtcgggataa cggacggctt atcgtaaagg aatatgaggg cgcctatcaa gctgtttcgc
     7261 tgtttattga aaatggccat acgaagatcg gcatgctgaa ggaaaccact ggatactatc
     7321 aattagaaga acgtttcaac ggatatcgtc atgcgctgaa agatcatggc ctgcctttta
     7381 atgggaaatt tgttgaacat ggagagttga ctgtccaagg tggatatact gcatcaaaaa
     7441 aattactcaa acataaagat atcacggcta ttttttgcgg gaatgatgcc atggcgatcg
     7501 gatgctatca ggcgatcgat gaattgggca aaaaaatccc agaagatatc tcagtcatag
     7561 gattcgatgg attaaagctg tcagaataca tgattccgca attgacgaca gtgcagcagc
     7621 caagttttga tattggattt tatgctgcac gtttcctgat cgatacgatc gaatttcctc
     7681 aaagaaaagt tccgaacaaa gtgttcgaaa caaaattaat tattcgtgag agcgtaaaag
     7741 tattgacaaa gggctaactc ggcggtatat tgcttatgta agcatttaca aaggctagtt
     7801 agtgcttttt ttatttttag taaaccagtt tactaaagtg gtttaccttt cctaaaaata
     7861 atgtataata ccgaatgaag acaaggagga tcaggcaatg cgaatggtgg atctgattga
     7921 aaaaaagagg gatggaaacg agctgtcgaa agaggaaatc gagtacatcg tcacgaacta
     7981 tacgaatgga aagattccag attatcaagt cagtgcatta ttgatggcaa ttttttatca
     8041 agacatgaca aatgaagaaa tcacgaactt aacattggcg attgctaatt ctggtgatgt
     8101 catcgatttg agttctcttg aaggaataaa ggtagataag cattcgacag gtggcgttgg
     8161 tgacacgacc acattgattt tagcccccct agtagctagt gtcggagtga cagtggctaa
     8221 aatgtctgga cgcggtctag gttacacagg tggtacacta gacaagctag aagcaattcc
     8281 cggatttcag atcgagcttt cggatgaaga atttgtacgc attgttaatg aaagtaaagt
     8341 agcggtaatt ggtcaatcag gaaacttggc accggcggac aaaaaattgt atgccttgcg
     8401 tgacgtcaca gcgaccgttg attctcttcc gctgattgcc agttcgatta tgagtaaaaa
     8461 aatcgcggca ggagcagatg caatcgtttt ggatgtgacc actggagacg gtgctttcat
     8521 gaagaacatc gaagatgcaa gacgtctggc aaagaccatg acgagcattg ggaaactggc
     8581 taaccgagaa acagtggcgg ttatctcaga tatgtcagaa cctcttggcg aagctatcgg
     8641 aaatagtcta gaagttgtcg aggcaatcga aacattacaa ggaaatggtc cagaagatct
     8701 agtagaaatg tgctacgcct taggaagcca aatggttgtt cttgctggca aagctaagac
     8761 aaccgatgaa gcaagaacgt tgcttcaaga agctctggaa tcaggtaaag cgttagcaaa
     8821 attcaaagaa atgatccaaa atcaaggggg agacccagcg atcgtggaac atccagaacg
     8881 aattttgact gcacgctaca caatggagct tcctgctaaa caaagcggag ttgtgtcaaa
     8941 aatcgtggca aatgaattag gaattgctgc aatgatgcta ggtgctgggc gtaaaaccaa
     9001 agaagaagat attgatcacg ctgttggatt gaaactgcat aaaaaaattg gtgacactgt
     9061 gacaaaaggt gagtcactat tgacgatcta tagtaacgac gaagaaatca cttctgtcat
     9121 tgaattatta tataaaaata ttgaaattgg tgaatcggca atgaagccaa cgttgattca
     9181 tgacatcatc acagaataaa ggaaaaaagc ggactttacc gttttaaaga aaaggggaaa
     9241 agaaatggaa ctaaaccgaa tgatcgacca tactattttg aaagcagacg catctaaaga
     9301 agctgtgatg caaattatcg aagaagcaaa aaaataccat ttttattctg tctgcatcaa
     9361 tccagcatgg gttgcgctag cgaaagaaca attacaaggt gagccagtag ccgtatgtac
     9421 agtaatcggg tttcctctag gagcaaacac acccgagaca aaagcatttg aaacaacaga
     9481 tgccatcaat aacggtgcag atgaagtcga tatggtcatc aatatcggtg ctttgaaatc
     9541 aaaggatgat caacaagtcc aaaaagatat cgaagcagtt gttgaagcag caaaagatcg
     9601 tgcgcttgtc aaagttatta ttgaaacatc gttattagat agagaagaaa tcatccgagc
     9661 ttgcgaaata gcaaaagctg caggcgctga ttttgtcaaa acatcaactg gtttctcaac
     9721 aggcggagca aaggtagagg atgtccgttt gatgcgtgaa actgtaggac cagaaatggg
     9781 tgtcaaagct tccggcggta tccataacga agaagaagca atggcaatga tcgaagctgg
     9841 tgctacaaga atcggaacaa gcgcaggagt agcaatcgta tctggttcaa ctggtgaagg
     9901 ctattaattt tttcaaagga gaagctcatg gcagaactca aacaagaatg gatcaacaca
     9961 gctattgaag cgttagataa agcctatgtt ccttattccc attttccggt aggcgcttgt
    10021 ctcgtaacgg aatcaggcaa gatttatcaa ggcatcaaca ttgagaatgc atcatttgga
    10081 ttaacgaatt gtgccgagcg cacagctttt ttcaaagcag tatctgaagg agaaaaaaca
    10141 tttacgcatc tagtcgtagc ggggcacacc cccgatccta tctctccttg tggtgcatgt
    10201 cgacaagtaa tggcagaatt ctgttcccct gatatgccgg ttacgctagt aggagacaat
    10261 ggtgtgacga aggcaacgac cgttcgtgca ctgctgcctt atgcatttac tgaaaaagat
    10321 ctataactca tgttcattca gtcagctgac tgttgaaaaa taaaacaatt attttaggag
    10381 gctttacaga aatgaaaaaa gcaaaattat ttggtttagg tgctgtcgca ctagcagcag
    10441 ggttattttt aggtgcatgc ggcaacaacg gctctactga ttcatccggt ggcaaaagct
    10501 catcagacac aacaactgca gcattgatca cagacacagg tggtgtagac gaccgttcat
    10561 tcaaccagtc agcttgggaa ggactagaaa aatgggggaa agatcaagga ctttcacgag
    10621 gaaatgatgg cttccaatat ttccaatctt caaatgaatc agattacatc ccaaatattg
    10681 accaagcatt gaatgctgga ttcaaaacga tttttggtat tggttataaa ctaaaaccag
    10741 cgatccaaga acaagcaaca aacaatacag gtacgaactt tgttattatc gatgatgtca
    10801 tcgatggatt agataacgtt gtatctgcaa ctttcaaaga caacgaagct tcttatcttg
    10861 caggtgttgc agcagcttat acgactgaaa caaacgtagt cggctttatc ggcggtgtaa
    10921 aaggtgaagt aatcgatcgt tttgatgctg gattcaaagc cggtgttgat gcaggtgcga
    10981 aagaattagg aaaagaaatc aaagtattga atcaatatgc tggtgacttc tctgctccag
    11041 ataaaggacg ttcaattgct caaggtatgt atgcacaaaa cgcagatatc atcttccatg
    11101 cttcaggcgg tacaggtaac ggtgtattcc aagaagcaaa atcattgaac gaatcaggcg
    11161 acaaaaaagt ttgggttatc ggtgttgacc gcgaccaatc agacgaaggc gaatacacat
    11221 taaacggcga aaagaaaaac ttcacattga catctacact gaaagcagtt ggaacagtag
    11281 tagaagattt ggcacaaaaa tcagcggacg gtaaattccc tggcggcgaa cacactgttt
    11341 atggattgaa agaagacggt gttggtctaa cagaaggaca actttctgac gaagcgaaaa
    11401 aagcagttga tgaagcaaaa gaaaaaatca tttcaggtga cgtaaaagtt ccagaaactc
    11461 ctgaagaaaa ctaaaaccat ttctatagcc ttgttagaca catgtttgac aaggctatgg
    11521 tttgattaga acaagaattg ttcatttatc caagctgcct tgtgacagtt tggataaggg
    11581 aacaactccc tgaagtaatg tttgtttcaa aagaagaacg ctcactaagc gtgaatctta
    11641 gtcaatcgtt gccctagata gaaatcgtta ggatgtgaaa aaaaggtggt tcaagaaaat
    11701 attgtcatag aaatgcagaa tattaccaaa gaatttggaa cattcaaggc aaatgacaac
    11761 atcaatttac aagtaaaggc aggagaaatt catgcgttgc ttggagaaaa cggtgctggc
    11821 aaatctacat tgatgaacgt gctttccgga ttattagagc cgacatcagg gaaaattttg
    11881 atgcgtggga aagaagtaaa gatcacaagt ccgaccaaag ccaaccaatt agggattggg
    11941 atggtccatc agcactttat gcttgttgat gcctttactg taacagaaaa catcgtgttg
    12001 ggaagcgagc ctagtcgcgc agggatgctt gaccataaaa aagcacgaaa agagatccaa
    12061 aaagtttctg aacagtatgg attgtcagtc aacccagatg cttacgttcg tgatatttca
    12121 gttggaatgg aacaacgggt agaaatttta aaaacacttt accgaggagc agatgtactg
    12181 atttttgatg agccgacagc tgtattgacc cctcaagaaa ttgatgaatt aatcgtgatc
    12241 atgaaggaat tagtcaaaga aggcaagtcg atcattttga ttacgcataa gttagatgaa
    12301 atcaaagcag tagctgaccg ctgtacagtt atccgccgtg gaaaaggaat cggtacagtc
    12361 aacgttaaag acgtaacctc acaacaatta gccgatatga tggttggaag aacggtttca
    12421 ttcaaaacga tgaaaaaaga agcgaagcct caagaagtcg ttttgtctat tgaaaatcta
    12481 gtggtgaaag aaaatcgtgg attagaagcc gtgaaaaact tgaacttaga ggttcgtgct
    12541 ggcgaagtac ttggtatcgc tggaatcgat ggaaacgggc agtcggagtt gatccaagct
    12601 ttgactggtt tgcgaaaggc agaaagcgga catatcaagc taaaagggga agacatcact
    12661 aataaaaaac cccgaaaaat cactgaacat ggtgtaggac acgtgccaga agaccgtcat
    12721 aaatacgggt tagtcctaga tatgacatta tctgaaaata tcgcactaca aacctatcat
    12781 caaaaacctt acagtaaaaa cggcatgctg aattattcag taatgaatga acatgccaga
    12841 gaattgatcg aagaatatga tgttcgaaca acgaatgaac ttgttcccgc aaaagcttta
    12901 tcaggcggaa atcagcaaaa agcaattatc gctcgtgaag tcgaccgaga tcctgatctg
    12961 ttgattgttg caaatccaac tcgtggactg gatgtaggtg cgatcgaatt tattcataaa
    13021 cgtctgatcg aacaaaggga caaatacaaa gcagtgttac tgatcagttt cgaattagaa
    13081 gaaattttaa atgtttcgga tcgtattgct gttatccatg aaggagaaat tgtcggtatc
    13141 gttgatccga aagaaacatc tgaaaatgaa ttaggtctat tgatggcggg ctatacctta
    13201 gaagaagcac gaaaagagtt agaaaagggg gctggcgcac atgaatgatc gcagcgaaaa
    13261 attccgtaat ttattagtgc ctgtactttc agttgtttta ggattgatct taggcgcgat
    13321 attgatggct gcttttggtt ttcagcccat ccaaggttat tcttctatgc tagatgcagc
    13381 ccttggttcg caaagaggga taggtgaaac gttaaggcaa gcaacgccgt tgatttttac
    13441 agcattaggt ttttctgtag ctaactcagc tgggtttttc aatattgggc tttctggaca
    13501 agcactttgc gggtggatcg tcagtgtctg ggtggcactt gcgtttccag atctgccaaa
    13561 agttgttttg cttccgttat gcgtgatctt aggtgcctta gctggtgcga tggcagcggc
    13621 tgttccaggt ttacttcgag cattttttgg aacaagtgaa gtgatcgtaa caatcatgat
    13681 gaactatatc ttattatacg taagcacatt tgctttacat gaagtgatgc ctgaaagcta
    13741 tcgctcaagt ttggactctt ccaatatgat cagtcaaaat gcctcactgc ggttaccatg
    13801 gttaacacaa gcttttggcg gctctcgcgt aaactcagga ttatttttag caatcatcgc
    13861 gcttattttc gtttggctgc tgatgaagaa aacgacttta ggatttgaaa tccgttctgt
    13921 tggattgaat ccttttgctt ctgagtatgc aggaatgagt agtaaacgta cgatcgtttt
    13981 gtcgatggtc atttctggcg ctttggcagg gcttggcggt gtggttgaag gattaggaac
    14041 gtatcaaaac ttctttgttc aaacgacttc gctatctatt ggatttgacg gaatggctgt
    14101 ttccttgctt ggttctggat cagcaatcgg tattctttta tcagcgatct tgttcagtat
    14161 tttgaagatc ggtggacttg gcatgcagac aggagcaggt gttccgtttg agatcgtcaa
    14221 tgtatcgatt gctttgatta tcttctttgt cgggatcaac ttcctgattc gtttcgtcat
    14281 ggctaaagtc ttccaaggga aaaaacaaga agagatcgtt gcgacaatag aatcgaaacc
    14341 aacgaatcaa aaccatgaag ggggcgaact ataatgtcga ctatcgaatt aattgccttg
    14401 atccttactt ctacactggt ttattcaaca ccgctaattc taacgtcact aggcggaact
    14461 ttttctgaac gaagcgggat cgtaaacgta gggctagagg gaatcatggt catgggtgct
    14521 tttagttcca tcgttttcaa cttgacgatg agtccatcac ttggttctat gacaccatgg
    14581 atcgctattt tagtgggtgg cgtcgtaggt atcctatttt ctctgattca cgcagtagca
    14641 acagtcagtt tgcgtgctga ccatattatc agcgggacag taattaatct gatggcacct
    14701 gctttaggtg ttttcttgat caaagcatgg tatggtaaag gtcaaactga taacatcacg
    14761 cagaatctgg ggtatttttc tttcccagga ttagcagata ttccggtcat tggaccaatt
    14821 ttctttaaaa atacattctt acctgcttat tgtgctattt tgattgctgt cctagcttgg
    14881 gtgatcatct tcaagacgaa attcggcttg cgcctccgtt cagttggtga aaatcctcaa
    14941 gctgccgaca cattaggtat caatgtatat gctatgcgtt attcaggcgt actgatttct
    15001 ggcttgttag gcggtatggg cggtgctgtt tttgctcagt cgatttccgg taacttttca
    15061 gctggtacga tcgttggaca agggttcatc tccatggctg ctatgatctt tggtaagtgg
    15121 aatcctttag gggcaatggg tgcctcattg ttctttggtt ttgctcaaag cttgagtatc
    15181 attggcgcac aattaccagt tatttcttcg attccgccag tattgctgca aattgctcca
    15241 tatttgctga caatcattgt gctggttgtt ttcttaggga aagcttctgg tccaaaagca
    15301 aacgggaaga actatatcaa atcaaaataa tcttttagcg aataaaaagg gtgggttacg
    15361 ggaagtgttt ttcgtctcgc cctttttgtt ttaaaggcaa taaaaataaa aagaaagaag
    15421 gaataatcat gtttaaacgc gtacatctga tcgtcatgga ttcagtagga attggagaag
    15481 caccagatgc cgataagttc ggtgatgttg gttctgatac attaggacac attgcgaaag
    15541 aagcaggatt gaacgttcca catttagagc agcttggttt aggaacgatc cgtcctcttg
    15601 aaggtgtgga atcagtgaaa gatcatcaag gctatgcgac gaaattagaa gaagtttctg
    15661 tagggaaaga tacgatgaca ggccactggg aaatcatggg attgaatatc aaaactccat
    15721 ttcgtgtgtt tcctaatgga ttccctgatg atctgatcaa acaaatcgaa gaattttctg
    15781 gccgtaaagt cgtatgtaac aaaccttata gcggaacagc tgttattgac gattacggtg
    15841 aacaccaaat gaaaacaggc gatttaattg tatatacttc tgccgatcct gtccttcaga
    15901 tcgcggcaca tgaagaaatc attcctttag aagaattata tcggatttgc caattcgttc
    15961 gtgatataac aaaagatgat ccgtatatga ttggccgaat cattgcacgt ccgtacgtgg
    16021 gtgaaccagg agacttcaca cgaacaagca atcgccatga ttatgcgttg aatccattcg
    16081 gacacacagt ccttgattct ttgaaagaag caggaaagga cgtgattgct gttggaaaga
    16141 tcaacgatat tttcaacgga caaggaatca ctgaatctgt ccgcacgaaa agcaatatgg
    16201 atggcgttga ccagctgctg aaagttatga aaaacgattt ttcaggaatc agctttacca
    16261 atttagtaga ttttgatgca ttatacggtc atcgtcggga tgtagtcggc tatgcgcatg
    16321 cgattgaaga atttgactta cgaattccag aattgcttga agcgatggaa gaagacgact
    16381 tgctaatgat tacagccgat cacggaaacg atccgacgtt tactggtacc gatcatacgc
    16441 gagaatatgt accgttattg gtttacagta aaaaaatgaa agaacaaggg gcattgcctc
    16501 aaggatatta ttcagatatt tcagcaacga ttgctgagaa ctttagtgtt tcttcgactg
    16561 aaaatggcga aagcttcttg aaattattaa aataatcttt ttaaatagga gaaagcaaat
    16621 gaccaagtta agcgagatgt tacaagaaac gacccagttt atccttgata aaggagtaaa
    16681 agaaattgat tttggtttga ttctaggttc aggattaggt gaattagcag atgaaatcga
    16741 agatgctgta atgatttctt atgatgagat cccgttcttt cctgtttcga cagtagttgg
    16801 gcatgcagga aagctagttt atgggtctct ggcaggcaag aaagtgttag ccatgcaagg
    16861 gagattccac tactatgaag gtcattccat gcaaacagtg acctatccag tacgtgtgat
    16921 ggccggacta ggggcccatt cagtcattgt gactaatgca tgtggtggtg tcaacgaatc
    16981 atttgtacca ggagatttga tgctgattac cgatcatatc aattttactg gtgaaaatcc
    17041 tttgatcggt ccgaatgaag atgcaatggg ccctagattt ccagatatga gtcaagctta
    17101 tacgcctgac tatcaagcaa cagcaaaagc agcagcagaa aaactaggtt tggatcttaa
    17161 agaaggcgta tatatgggct attctggacc aacttatgaa acgcctgcgg agatccgaat
    17221 gtctcggaca ataggagccg atgcagtagg gatgtcgact gttccagaag tcatcgtagc
    17281 agctcacagc ggattaaaag tgttaggaat ctcttgtatt accaacttag cagcaggtat
    17341 gcaagcgaat ctgagccatg aagaagttgt agaaacaact cagagagtca aacaatcttt
    17401 taaagcattg gttaaagaaa cattgacatt attgtaatag gaggaacaat aaaatgagtg
    17461 tgcatattga agcgaaagaa ggcgagatcg ccgataagat tttacttccg ggagatcctt
    17521 tgcgagctaa atatattgcg gagacttttt tggaagatcc agtttgttac aatcaggtac
    17581 gcggtatgtt aggctttacc ggcaaatata aaggagaacg tatttctgtt caaggaacag
    17641 gaatgggaat gccatcagcc accatctatg cccatgaatt gatccagtcg tatggtgtaa
    17701 agaaattgat tcgagtaggt acatgcggtg cattgtcaaa agacgtccat gttcgtgatt
    17761 tagtactagc acaaggtgca gcgaccagtt cttcgatgat cgaaaaaaat ttccaagcct
    17821 ttcattttcc tccaatcagt gatttcaatc tattactgaa agcatatgag atagctaaag
    17881 aaaagggcta tacggttcat gtaggaaatg ttctttcgga agattctttc tacaaagatg
    17941 atttgacaga gacgttccaa ttagctgaac ttggcgtttt aggagtggaa atggaagcag
    18001 cggctcttta ttacttaggc gcgaaatacc atgtgcagac attaagtttg atgaccgtta
    18061 gcgaccattt gatcaccggt gaagaaacaa cagctgcaga acgtcaatct acgttcaatg
    18121 aaatgatcga agtaggattg gaaacagcga tagcagaata agaaggaaat gaaaaagctg
    18181 ccctgcatta agtaataaga acatccaagc aaaaatagct cctcatgttt ttgacttggc
    18241 tggacttatt attgcaaatg taagcttttg aacaccgttt accaagaggt tgtgaatctg
    18301 ccgtttaact ctgagtcaca aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat
    18361 attgtgactt gtgaccga
//
LOCUS       DAGCYL010000039        17715 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.040, whole genome shotgun sequence.
ACCESSION   DAGCYL010000039 DAGCYL010000000
VERSION     DAGCYL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.040"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            4..816
                     /locus_tag="MB216_001045"
     CDS             4..816
                     /locus_tag="MB216_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBT4525898.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="MB216_001046"
     CDS             813..1613
                     /locus_tag="MB216_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBT4525899.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="MB216_001047"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="MB216_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBT4525900.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="MB216_001048"
     CDS             3110..4819
                     /locus_tag="MB216_001048"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBT4525901.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="MB216_001049"
     CDS             5031..9383
                     /locus_tag="MB216_001049"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBT4525902.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="MB216_001050"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="MB216_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBT4525903.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="MB216_001051"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="MB216_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBT4525904.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="MB216_001052"
     CDS             11221..11514
                     /locus_tag="MB216_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBT4525905.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="MB216_001053"
     CDS             11511..11822
                     /locus_tag="MB216_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBT4525906.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="MB216_001054"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="MB216_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBT4525907.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="MB216_001055"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="MB216_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBT4525908.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="MB216_001056"
     CDS             complement(14623..14844)
                     /locus_tag="MB216_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4525909.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="MB216_001057"
     CDS             15292..17655
                     /locus_tag="MB216_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HBT4525910.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWELFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6036 a         3146 c         3903 g         4630 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc tttagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaatccaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcttttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaaagat tatccttcgc tgaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccgctcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgacc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgtatt
    17701 atttgtcaaa ttaag
//
LOCUS       DAGCYL010000040        17352 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.041, whole genome shotgun sequence.
ACCESSION   DAGCYL010000040 DAGCYL010000000
VERSION     DAGCYL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.041"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            51..863
                     /locus_tag="MB216_001058"
                     /pseudo
     CDS             51..863
                     /locus_tag="MB216_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            876..1604
                     /locus_tag="MB216_001059"
     CDS             876..1604
                     /locus_tag="MB216_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4525911.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(1673..3199)
                     /locus_tag="MB216_001060"
     CDS             complement(1673..3199)
                     /locus_tag="MB216_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBT4525912.1"
                     /translation="MKRLIGILAMLVLAGMLTACGGASGKAEDSKEKLSVMTTFYPMY
                     DFTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQK
                     TLQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVAN
                     IRDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLAL
                     EYGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGV
                     SLEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNG
                     YFEDEDVQDRSLSDWAGTWQTVDTYVEDGTFAPVFEYKEKLNQDKTAQEYKEYYTKGY
                     QTDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGA
                     FRYIQFRDHTIAPADSEHFHLYAGNESQEALLKEMENWPTFYPDTMDGREIAQEMLLH
                     "
     gene            3451..4632
                     /locus_tag="MB216_001061"
     CDS             3451..4632
                     /locus_tag="MB216_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4525913.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSVW
                     LVPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(4692..6320)
                     /gene="treC"
                     /locus_tag="MB216_001062"
     CDS             complement(4692..6320)
                     /gene="treC"
                     /locus_tag="MB216_001062"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBT4525914.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRITIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(6717..9428)
                     /locus_tag="MB216_001063"
     CDS             complement(6717..9428)
                     /locus_tag="MB216_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4525915.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEHNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKITEGKAQIAQGRTQ
                     LEDAKKQLAQQSAAIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKAAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWSYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(9438..10139)
                     /locus_tag="MB216_001064"
     CDS             complement(9438..10139)
                     /locus_tag="MB216_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4525916.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            10324..10926
                     /locus_tag="MB216_001065"
     CDS             10324..10926
                     /locus_tag="MB216_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4525917.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILTNN
                     DLLAKTLKRTAALKVLVNVDITTFSYLSHQTDAGGISALSAPAEFTDLLLKDSLKDTN
                     NMLTITKYNPSAFFGTSLDLQLRRRGIETIVLSGVATTNGVYATALDAFQHGYHVVLA
                     EDACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            11007..12020
                     /locus_tag="MB216_001066"
     CDS             11007..12020
                     /locus_tag="MB216_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBT4525918.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            12129..13046
                     /gene="rbsK"
                     /locus_tag="MB216_001067"
     CDS             12129..13046
                     /gene="rbsK"
                     /locus_tag="MB216_001067"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBT4525919.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            13074..13469
                     /gene="rbsD"
                     /locus_tag="MB216_001068"
     CDS             13074..13469
                     /gene="rbsD"
                     /locus_tag="MB216_001068"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBT4525920.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGSEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            13469..14842
                     /locus_tag="MB216_001069"
     CDS             13469..14842
                     /locus_tag="MB216_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4525921.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPIMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            15199..17343
                     /locus_tag="MB216_001070"
     CDS             15199..17343
                     /locus_tag="MB216_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBT4525922.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFEPVPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         5202 a         3434 c         3195 g         5521 t
ORIGIN      
        1 aggaactttg agatattctt taggatatta aaattaacta gcaccacggg tactatatca
       61 ttatttttta tacccgtttt tctggtgaaa cacctgatgt cttagctttg aatacgagtt
      121 ttttcattct atatctgtgt gttggcctga tcatcttggt ttattattgg aaaatctcta
      181 ataagaaatt agctgatcag ttattagagc aacaagtcag gatgcaacag gattatatca
      241 aagatctaga aaaaaactat caagacttga gagaattcaa acatgattac caaaatctgc
      301 ttttttctct tcacagctat atttcagaag gcgacttaga aggattacgg tcttattatg
      361 ctcaaacgat attacctaca cgccagatga tgaacctttt tccggccaat ctaagtatgt
      421 tggataatat gaaagttcct gagatccgta gtttactgtc attaaaactc atgatggcac
      481 aggaaaaagg gttggtcgtc cagcttcatt ttcctgagcc aatcgaactg gatacgaaac
      541 atacggtaaa ccttgtacgt atgcttggta ttatcttgga caatgcgatt gaaggagcta
      601 gtgctgcaaa gaagaaaaaa atcgaattga ccatactcaa gaagaatcat tccgtgatga
      661 ttcgtgtgat caatacaaca acaaatacat tgccgttaaa ccagttgaaa caaaaagggt
      721 tctcaacaaa aacaaatcca aaaaacgaag gccttggctt gtttatcttg gatgaattga
      781 caaagaccaa tccttatctt ttccttgaaa catcaattga aaaccagcgt ttttctcaaa
      841 cgatttatat acaaataaaa taaaggagta atgatatgat tcccatttat atttgtgaag
      901 acgatccaaa acaattagaa gttttatcaa cggtgatcaa aaatagaatc atgattgaaa
      961 acttagacag ttatgtccat atttctacct ctgatccgga agaacttctc aatgcagcca
     1021 agatcagaac ttcttctttt ggtatttact ttttagatat cgaactagaa gacagtgaaa
     1081 tggatggtgt agcgatcggt aaattgatcc gtgaattaga tcccttagga aaaataatct
     1141 atgtgacttc ccatacggaa atgtcgatga aaatcttaaa aagcaacatc gaaccgactg
     1201 attatatcgt caaagaagat ctttatgatt taaaagaaaa tgtcgagcat atcctgagca
     1261 aaattttcga agattttcaa atgtcccaga tccaaaaaga tatcttcaaa atagagttta
     1321 atgatgagat caaattttta ccgatcaaag acatccaata tttttctact tcgctaggag
     1381 caccacataa actggaagtc catttgactc atgcccaatt acaattctat gagaagatca
     1441 aagaaatcga gaaaatgcat cggaatttta tccgttgtca taaatcctat gttatcaata
     1501 cacaaaatgt gcggtcgatt aataaaaaaa ccagagaagt cacgttggca aacggcgaga
     1561 ccatcccagc ctctatccgt ggattaaaga aattgatttc ataaaaagaa ccgaatagtt
     1621 tggaccgact aaatgactag ccgatccaaa cccttcggtt cttttttgcc tatcagtgca
     1681 aaagcatttc ctgtgcaatt tcacgtccat ccattgtatc tggataaaat gttggccaat
     1741 tctccatttc tttcagcaat gcttcttgac tttcatttcc tgcatataaa tgaaaatgtt
     1801 cggagtcagc cggtgcaatc gtgtgatcac ggaattgaat atatctaaaa gcgccacttt
     1861 ccttatcttc tgcttcgaat agatacctta cgccacggtt tcctgcctcg taggtcaaga
     1921 tttctttccc tacataacga taatttgaac gtttcgttgt tccatcctta aacgtaaatg
     1981 ccatctcttt ctcactgatc ttgattgaag caatatctgt ttgatagccc ttcgtataat
     2041 attctttata ttcttgtgct gtcttatcct gattcaattt ttctttatat tcaaaaacag
     2101 gagcaaaagt cccatcttct acatacgtat ctactgtttg ccaagttcct gcccaatctg
     2161 aaagagagcg atcttgtaca tcctcgtctt caaaataacc gttgtagacg ctcttttctt
     2221 ttttgggggt caaagtatct tctagcgggt tttctgtctc tgtcgttttt ttcaacgctt
     2281 cgaggtttgc ttccatgata gaaagatagt tttctccatt ttctatttgt tcattcgtca
     2341 atccttccaa aggattcaat acttctaaag atacacctgc ttcgtttgct aacgttctgg
     2401 caatactatc tttggcgttt tcttcaaaat aaatatagtt gattccatgt tctttaacaa
     2461 agtgtttcag ttcagcaatt ctcgccgcag acggttcttc actagaagaa agaccagcaa
     2521 tcgatacttg attcaatcca tattctaatg ccagatagtt gaatgcagca tgttgcgtga
     2581 caaaagatct ttgttttgct tgcttaaagg tttcttgata aagctgatgg agtgcttgaa
     2641 gtttctcggt ataggctgct gcattttttg tccatacttc ctgtttttct gggtaagctt
     2701 caatcagctg atcacgtatg tttgctacct gcttgattgc taaacttgga gctagccata
     2761 catgtgggtc tagctcatgt gtatggcctt cttccccgtg ttcatggtca tgatcatgtc
     2821 cttcttcact tcccggaagt aaaagcatac cttctgtcgc ttttatcgta tggacattgc
     2881 cttcttgtaa tgttttttgg attgcgggga cccatgtttc catgttttca tcattataaa
     2941 caaagatatc tgtatcttgg atctttgcca tgtctttagc agaaggttca taatcgtggg
     3001 aatcagtccc tgctggaatc aataactcta cttctccttc atccccgacg attgcttttg
     3061 taaaatcata cataggataa aaggtcgtca tcacgctcaa tttttcttta ctgtcctcgg
     3121 ctttcccact tgccccgccg caagcagtta acatacctgc taaaaccagc atggccaata
     3181 tacctatcaa tcgtttcatt tatgacactc ctcttctcat cttctttcat ttgaatgttc
     3241 cgattcgcct tttacaaaac gtaatagtta cgttttagca acaggattta atttatcata
     3301 tggttaaaag aactgtcaag cgttttctta ttttaattga tgagaaagtt tatatgaatg
     3361 attgacgata tttttgcaat caacctctat cattttcctt ttatacccgc ttttgtctat
     3421 aatgaagtta tagataaagg aggaaaaaga gtgaaaaaag aaacagatac aacgaatcgt
     3481 ttgatccgct ttttaggagg aaaaacctct tactatgttt taggaatagt tatcttactt
     3541 gcgctgatca tttatttttt ccatcagatc tcgtttatat tcaagcctgt cggaatcatt
     3601 atcgctacta tcttgccccc attattgttt gcactgatct tgtattatct tttggaacca
     3661 gtcatcaaac gtttgagcaa gcacctttcg aggaacttgt cagtcattat cgtttatgtg
     3721 attattttat ttttattggg gcttggaagc gtatggcttg ttccttttct tgaagaacag
     3781 gcaaagacat tgatcagtag tttgccagat ttgtttcaag attttcaaga gagtctccga
     3841 caatttttag agaaaacacc ttttgccgat tcatttaatc aatttttcgc ttctattgac
     3901 gacgtgacgt ctgatatagc tggatttatt ggaaattatt gggaaagtgg cgcacaacgg
     3961 atcagcaaca ttttttcaac tgttacagct atttttatta ctttatttac tggtccaatt
     4021 gtcgcttttt ttcttttgag agatcctcaa aaattttatc agtcagtttt agcgattgtc
     4081 ccaccagcat ttcgtacaga ttttaaaaat ttgacaaaga ttgctaatca acaattgggt
     4141 tcatttctaa aggggcaaat cattgcttca tttatccttg gagcagtcta ctgggtttgt
     4201 ttcttactga tcggattaga atttgctagt gttttagcta tttctgctgg gttgttatgt
     4261 atcattccat atattgggcc gtttatcgcc ttctttccag gtttgatcat tgcattccaa
     4321 gactctactt ttatggctgt caaatttgtc attgtctggt ttgccgtaca acttctccac
     4381 ggtgacttgg tgattcctcg agtaatgggt gatcgtttac agatccatcc gattacgatc
     4441 ttaatcgttt tacttgtcat gggtgatcta atgggaattg tgggcgttat ttttggtatt
     4501 cctatctata cgttagtcaa attactcgtt acttttattt tccgtaaatt caaacaacgc
     4561 tacaataaat tttatggcga taaaggagag tatgaaaact cttcattttc tgaagaagat
     4621 tattttaaat aaaacacaaa acgtcgatct tacgagaata gctcgtagga acgacgtttt
     4681 gtgtttttgt tttaattttt cagcaaagca attgcctggt atggttttag ctccagactt
     4741 tgatgaagaa cagtatcgga ataattatcg ataagaatat tggcttctgt gaatccttct
     4801 gggatggtga tccttgtatc tttatcatag aaatttgtta atacaagcag ctgctgtcct
     4861 tcaaattggc gaagaaagcc ataaacttcc ggatgatttg cttcataagc atgataatct
     4921 ccttgtgcaa cgattggata tgtttttctc agccgaatca atttcttgta ataggacaaa
     4981 atagaatctt cagaagatag ttctttttct acattaatct cttctagctt gcctccaact
     5041 tttaaccaag gtttccctgt agtaaatcct gcttgttctg tgtctgacca ttgcatgggt
     5101 gttcgagagt tatcgcgaga ttttgctttg atccgacgaa acgcttcttt ttctgtgaat
     5161 ccttgttcaa ggagtaactt ataagcattc aaactttcta cgtcaatata gtcggaaatc
     5221 gatgaatagt ctgggtcgat catgccgatt tcttctccca tataaatata gggcgtccca
     5281 cggttcaaat ggatcatggt tgctagcatc tctgctcctt gttttcgata ctgatcgata
     5341 gctacaaatc gattgagcgc acgtggctga tcatgattat taaaaaatag agcattccag
     5401 ccgttaccct cactcatctt ttctccccat gtatgaaata gtcgctttaa ttctgcaaaa
     5461 tcaaatggag gagtagacca tttattccca ttttcataat cgactttcaa atgatggaaa
     5521 ttaaagacca ttgataattc ttctctacta ggttctgtat acaagataca attttcgatc
     5581 gtcgtagagc tcatttcacc gactgtgatg atagtggaat ccgtcccaaa cgttgcttgg
     5641 ttcaattctt ttagatacgt atgggtgatt ggtttgtccg tatattcagg tttgccatca
     5701 ttttctttgt tatttttcaa ttgttcgtct ttaccgataa cattgataac atcgaaacgg
     5761 aatcctttta cccctttttc catccaaaaa cgaacaactt tgaatagttc ctctcttact
     5821 gcttggttcc gccagttcaa gtcagcttgt gtccgatcat ataagtgcaa ataatatttc
     5881 cccgtatctc caaatggcga ccaagcactt ccaccaaatt ttgataccca atccgttggc
     5941 tcttctctta aaatgaagaa gtcctgataa tgctggtccc ctgctagtgc ttttttgaac
     6001 cattcatgtt ctgtcgaaac gtgattcagc accatatcga gcatgatttc tatccggtat
     6061 ttctttgctt cggctattag ctgttcgaat tcctccatcg taccaaagat aggatcgatg
     6121 gctgtgtaat cagagatatc ataaccgttg tctttttgag ggcttggata aatagggttt
     6181 agccaaatca tgtcgatgcc tagatcattc agatacggga gtttttcttt gattcctttt
     6241 aaatctccaa ttccatcacc attactgtct ttgtaagatt tgggatagat ttggtaaatg
     6301 actttgtctt taaatgtcat ggatatttac ctccattcaa ctgatttatt acctaccgca
     6361 acttatatat acaagttatc atttattatt ataactgaac agaaagcgtt tgtaaaattt
     6421 atctttatac attctaagag gtctgttgcc caataaaaaa caagagatca gacatgtatt
     6481 tgtctatcct cttgtttaaa atagcaaaat attcttgttt cttctataaa tggtgctcaa
     6541 aagcttttat ctacggcaat cagtcttctc aaatgtaaaa catgaggagc taattttatt
     6601 tgagtattcc ttttgctcga tgcaggacag ctttttcaca acctctgatt cacggtgttc
     6661 aattagcagc tgaccctcgt tattagctca aaacgctgat ttcacaacct ctgtttttat
     6721 tcattcgatt tcaacgcatc aatcatatcg atatgcttta atttgaaatg ggtaacaatc
     6781 atcacaataa ccgtaaatac gatagttaat cctgctgata agacataaga ccaccatgtg
     6841 atcactaatg gaaagatgac attctccata gacgcttgtt gcaagatgaa gtcagtcaat
     6901 atatatccta caccaaatcc tccgatgatt cctaaaagag taaagatgat attttctcga
     6961 acaatgtaca tcgtgacttc tttatcaaaa aatccaagga cttttattgt agataattcg
     7021 cgtacacgct cggaaatatt gatattagtt agattataca agacgataaa agccagcagt
     7081 cctgataata cgacaaagat taagacaacc gagtctaagt tcgccattga ttcttcttgc
     7141 gtttcgattt gtttggacat gaaagacgta tttgtcacag cgtctgtggc aagcagtttc
     7201 tcggaaagca tttcttcttc ttttgttgtc atcttatctg tacgcagcaa aaatgcatta
     7261 tccatcatcg attgatcagt aactgaagca taatattcag gggacatata aataaagcta
     7321 cctagatagt ttttcacgat ttcttttact tcgactttcc attctttttg atcttcatca
     7381 taaagcgaaa tatgatctcc tgtagataag tcaaatagtt ctgctgcttt ttgcgtaata
     7441 gcgattcctg ttttaggcaa ggagcgtggg ttctcttcaa gatcagttaa atggataaaa
     7501 cgatccaaat cagctgtttt acttggtacg atcactgtca gactttgcga agcatgtcct
     7561 ttttttctca attctacggt ttgagtatga atagctagga tctcatttac tttttcttgc
     7621 ttctgcagca tatccgctgc tttttgacga ctatcttccg tggatgaatc caatgtaacg
     7681 attgcttgat agtcaatgat cggtccaaat tgtttagctg agacagaact tagagaatct
     7741 ttcaatccca cgcctgcaac catcagcccg gcacatccag caattccgat aatcgccata
     7801 agcattcttg acttatatcg gaagagattt cggtaactga ttttttgatt aaagctaagg
     7861 cgtgaccaca gcggagtgat gtattcaaga aaaatgcgtt tccctggctt cggtgctttc
     7921 ggttgaagca atgcagcagg ccgttctctt aaatctttga ataaaacgat cattgcagcg
     7981 ccaaatgcgg caatgaagaa tgcggcagct gcttgtaaaa tcggcggcca gtcataaaaa
     8041 ataagcgcac tcccaatatc ataccgctcg ttagacagct caaaaatgat ccttggcaaa
     8101 agattcgttc ctaatatcgt tcctaaaaca atgcctatcc ctgaggctag acttgcatag
     8161 attgcatatt tttttgcgat ttcaactttc gtataaccta acgcctttaa tgtcccgatt
     8221 tccctgcggt tttcttctac catacgtgtc attgtcgtaa aggtgatcaa tgcggcaata
     8281 aagaaaaaga aaacaggaaa aacattggca atcgcagcga tacgatcaga cagatcacca
     8341 aattcttgaa aaccgacatt ttctgaacgt tcattgtata ggtatgatgg tttagttaaa
     8401 ttttcaattt tttctgcatt tcgattcaat tcttcctctg cagaggccag ttcacgttct
     8461 ttctcaacta attgcctttc ttgttcagca agttgagctt cttgttgaga taatgtttga
     8521 acagcgattg ttcgttgcgc ttcaggaagc tgctggatag ctgctgactg ttgcgctaat
     8581 tgttttttag catcttctaa ctgtgttctt ccctgtgcta tttgtgcttt gccttctgtt
     8641 attttttgct gtgctggttc taattcttcg tttgccgatt ttttcagctc ttctaagcgt
     8701 tcttcagggc gatcttgtaa atacttttct gccttctctt gattgtgttc cattttatct
     8761 ttgtaagctt tgctgtacgt attcaaattc tttacgtttg taaaatcaag atataagaca
     8821 gaataaatat ctgttttaaa aacggattca ggtaaataga caaaataatc gacgctacca
     8881 tttccgacgt tcgcgtatcc tctctccaaa ttgttgatga acaatggaga acggacaaag
     8941 cccgtgatgg tatactcttt ttctttcaat tcatctgaat caatctgata aatatcaccg
     9001 attttatacc cttcatcttc cgctttttca tccaatacaa gctggtttgg tttttccggg
     9061 agttttccat cagttaattg tagcttgttc tgcttgttat ttttgttata agaaaatatt
     9121 tgaatcactt cgtttttacc agtattggaa taatagatct gttttcctgt ttctactttt
     9181 gcatctggga tcttttctgc ttgagcaata tctttttgtg tcaagccacc agtagaaacg
     9241 atttgcaaat cagaaagtcc tgctgtacca acatagtggt ccatcgtttt ttgcatgaca
     9301 ggtccagcag ttttgacacc tacaaataaa agtgttccta gcatgacaat aaggatgatt
     9361 gcaatgaatc gtcctttaga ttgccgaatt tcgcgaaaac ctgctttaag atatgttctt
     9421 gttttcataa aatcccccta ccatacaatc tcatcaatgg atgaaggatg atcatttaat
     9481 tcaacagacc ttactttagc atcattaatg tgaatgactc tatcagcgat gggcgctaaa
     9541 gcagaattat gtgtaatgat caaaacggta ttgccgtgtt gtctgctagc gttttgcaat
     9601 aatttcaata cttgcttgcc cgtttcaaaa tccagcgcgc ctgtcggttc atcacataaa
     9661 agaagtttgg gatttttagc tagtgcacga gcaattgaaa ccctctgctg ttcgcctcct
     9721 gataattggg acggaaagtt attcaatcga tgagctaggc ccacttgcct caatacctct
     9781 acaggatcaa gcgcatcagg agaaacttcc gttgccaatt ccacattttc ttttgctgtt
     9841 agattaggaa cgagattgta gaattggaaa acgaacccta catctgttcg tcggtaagca
     9901 gtaagctgtt tatctgaaaa ttgcgcaata tctgtgtcat caataatgat ctgaccttca
     9961 tcaggtgtat ccatcccacc taaaatatta agaatcgttg actttcctgc tccacttgga
    10021 ccaagtataa tcactaattc gcctttttca attgaaaaag aaatatcgtc atttgctgta
    10081 atggtcgttt ctcccatttt gtatcgtttg tattcatttt ttacttcgac atacttcact
    10141 ctgattcctc cattttcacg atatttctcg ctatatctat gcatcgaatg cgttttctca
    10201 tacttattta ttttaccact gaatttctct agactaaaga aaggaatcct ttatttagtt
    10261 gacaaataaa cgatttatga tttacttaca taaaaaacat tgttatttga aagaaggaac
    10321 atcatgaatg atttaaagga aaatatgatt tttgatttat caaaaactgc ttttgtcgcg
    10381 atcgacatgc aaaaagggat cgtaaataac ggtacactct ctccacacac tgcacaatct
    10441 attctgacaa acaatgattt gttagcaaaa acattgaaac gtaccgctgc cttgaaagtt
    10501 ttagtgaatg tagacatcac tactttctct tatctaagtc atcagacaga tgctggagga
    10561 atatctgctt tgtctgctcc ggcagaattc actgacttgt tgttgaagga ttccttgaaa
    10621 gatacaaaca atatgttgac tatcacaaaa tacaatccaa gtgccttttt tggtacttct
    10681 cttgatcttc aacttcgccg gcgtggaatc gaaactatcg ttttaagcgg tgtagcgaca
    10741 acaaacggtg tatatgctac tgcattagat gccttccaac atgggtatca tgttgtactt
    10801 gctgaagatg cttgcagtga tcgtgacaaa gaatcccatc aattgttcat aaaaaagata
    10861 tttccgaaaa ctgcacgagt acgctcaacg aaacaaataa tcgaagctat ccaacaaagc
    10921 aaataagtac tggcacacgt gattttcatt tacttctttt tgatttcatg ggaaaataac
    10981 cacaggattt agataggagt atgaacatgg tgaaaaaaaa gatcacaatc aaagaagtcg
    11041 ctaaactttc cggcgtttca atcacaactg tctccttgat attaaatgga aatacggaga
    11101 aattcagtcc gaaaactgtt cagaaagttt tagctgccaa agaagaatta ggctatcagc
    11161 ctgactattt tgctagacaa atgattacca aggaaacgaa aacaattggt gtactcgttc
    11221 ctgatatcac gaatccattc ttcagtacac tgatgcgagg aattgaggat atcttgtata
    11281 agcagaattt cgtcacgata ttatgtaatg ctgattctga tcatcaaaaa gaaatcgaat
    11341 atctagccga gctcacacgt cgaggtgtgg atggattcat tatcgcgact tctgctgtct
    11401 ctacagatgc aatcaacgaa aatttgaaaa aacaaggccg gcccttcatt gttttggatc
    11461 agaaaaaatc tgaaggattt agtgatgccg tacgaacaga cgattttcgc ggtggttacc
    11521 tagcgggcat gcacttgctt agtctaggtc atcaaacgat cgcccttgtc tatcctgaaa
    11581 atccgccgga aaatgtccac gctcggattg aggggtttaa atctgctctt gatgtttacc
    11641 aaatctctca cgaccaactg atcctcttac ctacacagtt ctcaaaacaa ggtggctacc
    11701 aaatcacagc agaacttctt gattctgctg caactggtgt gtttgcttta aatgatgaat
    11761 tagcttttgg attatatcgc ggattagagg aatctggaaa aagcattcct gaagactaca
    11821 gcattattgg ctatgataat atagatatgt gcgagtatat caagccgaaa ctgacaacga
    11881 tcgcacaacc gatcttcgaa ttgggacaaa catctgccaa attattgtta gatcggatcc
    11941 aattcccaga aaaagaatgg gaagaaaaaa gattacctgt acgattagaa aaaagatttt
    12001 caactgcacc cttgaaatag ctttcaaggt gtgctatatt tttatcgaac tattaaaacg
    12061 ttttactaaa acgttttaaa aaataatttt tcccatcaaa ataaatgaga tagttggagg
    12121 tttgtaaaat gaacacggtt acagtaattg gaagtattaa tatggacact acccttcgtt
    12181 taaccaacat gcccaagcca ggcgaaacaa tgcatgcgca tgagattttt cacgctggcg
    12241 gaggtaaagg tgccaatcaa gcagtggcag ccaaacgttc aggcgcacgg acatctttta
    12301 ttggaggcgt tggggctgac agcgaaggac aacagttatt agatctactg acaaaagaaa
    12361 acatcgatac atctgggata gctgaaatcc aaggcgccac aaccggacaa gcaatgatta
    12421 tggtagacgc agcaggagaa aatagtatct tgatccatgc aggtgcaaat aatgctttcc
    12481 atgaacaaga agtcctaaaa aacaagcaac tgattacaaa cagtgacttt attattgctc
    12541 agtttgaaag tagcttagat gcgacgatcc tagctttttc tattgcaaaa gacgcaggaa
    12601 aaacaacgat cctgaatcct gcaccagctc gtgaaacgat ccctactgaa ctacttgaaa
    12661 aaacagatat cattatacca aatgaaacag aaacagaaat cattacagga atcagagtga
    12721 cagatcataa cagtttggtc gcagcagccg aaaaactcca tgaattaggt attggaacag
    12781 taatcattac actaggcagt gccggcgctt tctatcatac agaaaaagaa cacggtatcg
    12841 tccctgcttt taaagtggat gccgttgaca caacagccgc aggtgataca tttattggcg
    12901 cattaagcag cacacttcag ccagacctta gtaacttgaa agaagcgatc ctttatggaa
    12961 atctagcctc ttctgttgct gtgcagtctt atggtgcgca accttctatc ccttaccgcg
    13021 aagctttaga acaatcagca gtctaaaatt aacaatgaga ggaagaaaca aacatgaaaa
    13081 aaggaaaagt aatcaattca gatatttcac gagtaatcgc acaaatgggg cactttgata
    13141 aattaagtat cggggatgca ggcatgcctg tcccctttgg tagtgaaaaa atagatttgg
    13201 ctgtctcaaa cggtcttcca agctttttat ctgttttaga aaacgtttta gaagaacttg
    13261 aggtacaatc gattgaattg gcggaagaaa taaagacaat gaacccggaa atattggaaa
    13321 aaatcaaatt attgttacca gatacaccga ttagctttat cccacatgaa gaaatgaaaa
    13381 aagagttgaa taactgtcat gcatttattc gtacaggcga aatgacacct tacgccaata
    13441 ttatattagt gagtggagtg gtcttctaat ggaaacgaca atgaatcctg aacagatggt
    13501 actgtccgag cagattcata tgctgattga acagttggga ggaaaagaca atattgattc
    13561 tgtccttcca tgtgcgactc ggcttcgtat caaattgaaa aaacagccag tagccaatgc
    13621 agaaaaatta aaacagcaaa tcccttccat taagtatttc attttactcg atagatatgc
    13681 agaaataatc atggatcagg gattggctcc tgcttacaag gaattaacgc gtcaaatcga
    13741 tcattcagct cctaaagaaa agctaaatcc gatcgaaaaa ctatttggtt tgatatcgga
    13801 aatctttacg ccgatcattc cgctgtttgc cggttctggt atattaaaag gtctggttgt
    13861 tttagcgact acgattggct ggcttagtga aacaagcgga acttatcata ttttgtctgc
    13921 tgcgtcgaat gccgtctttt attttctgcc aatctttctt gctttttctg ccgccaaaac
    13981 gtttaaagtg aatggatata ttgcggctgt cattgctgca gcactgattg aaccgaatct
    14041 gacctcttta ctttcagaca gtggcaaagc agctactgat tttctgggga ttcctgtagt
    14101 actgatgcag tatacgtcta ctgtcatgcc ggcgatttta ggtattttct tttattcttt
    14161 tgttgaaaaa ttcctcaaaa aatatattcg cgaaaatatg caattagtat ttgtaccttt
    14221 actttcatta gttatcacgg ttcctttgac gttaatggta ttcgggcctg cgggtgttta
    14281 ccttggcgaa ggtttagctg ccgcaattac ttggatgatg gatattaacg gaccgatcag
    14341 cggcgcaatc atcgctggtg gttggaatat tttggtaata tttggtatcc aatgggctgt
    14401 aaaccctatc atgatcagta atatctctgc ttatgggttc gaccgaatcg ttccgttgac
    14461 aggagcagcc aattttggaa tggctggtgc agctttaggt gtatttctac gaagtaaacg
    14521 ttcaaaaact cgttcgatct ccggttctgc ttttgcttcg attttacttg cgggagtgac
    14581 cgaaccaact gtttacggaa ttgcgatccc attgaaaaaa ccatttgttg ctgcttgcat
    14641 tggtgcagct gctggtggag ctgtaatggg atttgcccaa gtgaaagcga tcgcttttgt
    14701 atttggtagt ctgaccacat tgcctgcatt catttccggt acttttttct ggtacttagc
    14761 aggactggct gttagtctag tcgtagcaat gattactact ctcgttagcg ggtttgatga
    14821 agacctaatg tcttatgaat agttaatgaa aaagactgaa gtatgagatg tttatcacat
    14881 atttcagtct ttttcttttt aggatcatta tttttataaa tttttgtgat actttccaaa
    14941 ctttggtata atttacatat gcataaccat ttttatcccc acttgtttat ccccaaacaa
    15001 aggtggtcta tgcaataaaa tgaacgtcaa tgaaaaacga atgaatgctc gttgcttatt
    15061 tacaaattgt tttatttaga atacacagac agatttatga aaagaaaaag cttttcgaca
    15121 cgattatctg ttccaaaaaa tgtctaatga atgctgaata agacagaaaa tcaatgaaag
    15181 gaaaaggact agtttattat gaaaaataaa aaaacgatac gtttgctagc tgctacttgg
    15241 ctgattgctc cgtctgtttt gtcgaccaat accgtatttg ccgtagataa tcaaacagct
    15301 gatcaaacag tcacaacaga tacgactaca tccagctcag ctactagtga aacaacggat
    15361 tcttttacag aagacccaac tggaacaact aactcaacaa caggatcgac cgagccaact
    15421 gaaattgatc cggctgatcc atcagaaaat gaaaagccag ccgagacata ttcccttaca
    15481 attgctcctt ctttgattgg taagatccaa ctgactgctt cgacagcgga ttcccctgaa
    15541 cagcaacctt tggatattac ggaaagtgga acggtagaag gtttattagc ggaaacgaag
    15601 gtaacttaca ctattacgcc agctgaaggt gaacaaattg cttcagtcgc tgttaatggt
    15661 gccaaagaag taaattatga tgcaaagacc tttacagtag gtacgaacaa tttatctatt
    15721 actgcagaat ttgaacctgt accggatact tcaacagaga aaccaactga tcctagtgaa
    15781 gaagtcccta gtacaccaga tccggaaacg ccaaacaaac cagatgtggg taataacaac
    15841 aataataatg gttcaaataa ttcaaacaat aataacaata attcgaacag cgggaattct
    15901 tcgaatcaaa caaacccatc tggatcgaat aacaatggaa atactcagag agaaccaaac
    15961 catccaaaca atggttcatc aaatggcgga acgaacaatg cttcatctcg tccaaataca
    16021 gcaaatcaaa gcggtataga gaatccatct tctgcttctt ctgattttgt tgtaaaatca
    16081 ccaattgatg ctgtattgcc tacaaataca acgaatgtcc aacaagcaat cgttcgagaa
    16141 gcattcaagc atcttggaaa accatatgta tggggagcaa aaggtccaaa tacttttgac
    16201 tgttctggtt tgacttatta cgtatatatg aaagcaaccg gtcattatat cggtggctgg
    16261 acaggcgaac agcaatatgc aggtacacag attccagtaa gtcaggccca acctggtgat
    16321 ctcgtcttct ggggaccttc ttctggtgtt acccaccacg taggtatcta tattgggaac
    16381 ggacaattta tccatgcgcc tcaaccagga gataaagttc gagtaacttc tatttcagat
    16441 ttcacgcctg attttgctgt ccgagtaaac ttagcaggtt tacctgctgc ttcaggttcg
    16501 ctgacaggcg gctcatctat tttagatgga ttagatggct cgttccattt cagtcaaaac
    16561 caaacaactg atcagttctt gaaaaagatt gcagatgatg cacaggaaat cgggcaaaaa
    16621 gaaggaatct acgcatcagt catgatggct caagctatcc ttgaaagtgg atctggaaac
    16681 agcttattat caagcgaacc aaatcataac ctttttggta tcaaaggatc atataaagga
    16741 agcagcgtga ccttcaatac acttgaacaa gatagttccg gacaaagcta ccaaatccgc
    16801 gcacaattcc gtaaatatcc ttcttacaag gaaagtttgg aagactatgc cgacttgatc
    16861 aaaaatggac tgactggcaa tcctgatttc tacaaaccaa cttggaagtc ggaaacaaaa
    16921 gactacaaag aagcaaccaa atatttggaa ggacgttatg ctacagatcg ccaatattca
    16981 caaaaattga acgcgatcat cgaagcgtac gacctgacaa aatatgatga accgaaaaaa
    17041 gaagcagcaa caactgaaga agcaaacgct gaaatcctca atatgtctaa gcgattcgat
    17101 gtgccaatca aatggacaac agatggcgtg ccaatcacag gattagaatt ttcaacgata
    17161 aaacgcgtca acaacgcttc ttccatcttc cgtgtcgctt caatctggga tttgtggaac
    17221 aattttacgg caagacagat accagaaaca actactcgta cagttaaagc tgcaactgtt
    17281 cctgtactaa ccttgttaag tttagacaaa tcattctggc ttgatctgcc tttaaagaaa
    17341 tagaataaat ta
//
LOCUS       DAGCYL010000041        17349 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.042, whole genome shotgun sequence.
ACCESSION   DAGCYL010000041 DAGCYL010000000
VERSION     DAGCYL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.042"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(39..584)
                     /locus_tag="MB216_001071"
     CDS             complement(39..584)
                     /locus_tag="MB216_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4525923.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(596..1288)
                     /locus_tag="MB216_001072"
     CDS             complement(596..1288)
                     /locus_tag="MB216_001072"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBT4525924.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1307..1591)
                     /locus_tag="MB216_001073"
     CDS             complement(1307..1591)
                     /locus_tag="MB216_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525925.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1604..2167)
                     /locus_tag="MB216_001074"
     CDS             complement(1604..2167)
                     /locus_tag="MB216_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBT4525926.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2395..3057
                     /locus_tag="MB216_001075"
     CDS             2395..3057
                     /locus_tag="MB216_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBT4525927.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3181..4377
                     /locus_tag="MB216_001076"
     CDS             3181..4377
                     /locus_tag="MB216_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBT4525928.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4478..5854)
                     /locus_tag="MB216_001077"
     CDS             complement(4478..5854)
                     /locus_tag="MB216_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4525929.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5891..6757)
                     /locus_tag="MB216_001078"
     CDS             complement(5891..6757)
                     /locus_tag="MB216_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBT4525930.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6846..7871)
                     /locus_tag="MB216_001079"
     CDS             complement(6846..7871)
                     /locus_tag="MB216_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBT4525931.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7889..8563)
                     /locus_tag="MB216_001080"
     CDS             complement(7889..8563)
                     /locus_tag="MB216_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4525932.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8747..9640)
                     /locus_tag="MB216_001081"
     CDS             complement(8747..9640)
                     /locus_tag="MB216_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4525933.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9714..11411)
                     /locus_tag="MB216_001082"
     CDS             complement(9714..11411)
                     /locus_tag="MB216_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBT4525934.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11404..12717)
                     /locus_tag="MB216_001083"
     CDS             complement(11404..12717)
                     /locus_tag="MB216_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBT4525935.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12717..16754)
                     /locus_tag="MB216_001084"
     CDS             complement(12717..16754)
                     /locus_tag="MB216_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBT4525936.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTAINRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16767..17225)
                     /locus_tag="MB216_001085"
     CDS             complement(16767..17225)
                     /locus_tag="MB216_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4525937.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4865 a         3896 c         3013 g         5575 t
ORIGIN      
        1 attttgtgct ttttatttta tcaaatcact ttgttttttt ataaaatttc tgcgcctagt
       61 gtggactcaa aatgtctcaa tgcccattca tgtccaacgg gatcgaaact tgccacggaa
      121 tctttgaaaa tatggagttt gtatcctaga ttatacgcat cgactgctgt atgcaacaca
      181 cagatatctg tacatacgcc ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg
      241 atgtctagat ctgttccact aaaagcggaa taatgccgtt tgtctatcca ataaacattt
      301 tcctcttgct catagtcttg gtataaactt tttaaagagc cgtacaattc ccgtccaccc
      361 gttcctatca cattatgcgg aggaaataag cggttctccg gatgaaagga atcttttgga
      421 tcatgcgcat caatggcaaa aacgacaaaa tccttttgat caataaactt ctttgtatga
      481 gcagtcagct ttttttcaat tacttgtcct gctgtcccag tagtcagctt tccatcctct
      541 gcaacaaaat catatgtgta gtcaatcgag atcaatgcgt tcatcttagt cctccttata
      601 cgagatgttt gacaaattcg atcaccattt ctgctgatcg tttgcctgcg tcttcgataa
      661 attcgtcaaa gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca cgtacgatca
      721 aaaatgggat attgaactga cgagctgttt gtccaacagc ggctccttcc atttcgcaag
      781 ctaatgcttc agggaaattc gttaagattt ctttgatttt atctggcgag tcgacaaatg
      841 tatcacctgt aacgatcaat ccttcttttg cgttcaaatt cgttgctttt gctgctttaa
      901 ccatttcact gcgcaaatat gtgcttgctt cataatatag aggcattcct ggtaattgtc
      961 ctgggttgta gccaaatcct gttgcatctg catcaaaata agctactttg tctgatataa
     1021 cgatgtcacc tacttgtaag ccttcaccga ttccgccagc tgaacctgta ttgatcacca
     1081 tgttcacgcc gtattgttgg atcaacagac ttgtggtaat agatgctaag acttttccta
     1141 taccggaacg tacgacgatc acttcgtggt tacctaatga acctgaaata aataaagcgc
     1201 ctgcgcgttc ccatgataat ggttcgctta ggttctctct caaaattttt acttcttctt
     1261 ccattgcgcc gataataccg attttcatct ttttaattcc ttctttctat ataaatgcga
     1321 ttgctaaaaa aaccacacat aacattagga taacaataat aagagatttc atcaaaaaag
     1381 agttccattt ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt ggtttattct
     1441 ttttgctttc tttacgataa aagctggcaa tctttttttc ttcttgctga taagctgttt
     1501 ctgcttttcg ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt
     1561 cgcttcgcgt aataagcggt cctttggcca taagccgatc cctctacttt cctttggtca
     1621 atagttccca gtattgaatc gcataaatcg tctttgcatc acaaatcaac tgatctttca
     1681 tcgcttgttg cgcctcttca agagttagat gatacaactc cagtacttcg tcttcatctt
     1741 gtgctaaagg attctcgacc ttctctaccc cttgtgcatg ataaatatga aggacttcat
     1801 ttgcaaaacc tggtgaaaga tacatcgaag taagatggct cagcgacttc gcacgatagc
     1861 cagtttcttc ttctaattct ctagctgctg tcatttcggg attttgtcct tctcctggat
     1921 cgatttttcc agctggaatc tccaaaatca ctttttctaa aggttttcga aattgtttga
     1981 caagaagcag acgatcttgt tcgtcaaaag caatgatccc gacaccgcca ggatgaaata
     2041 ccagttctct cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa
     2101 taatttttcc gctatagatt tctttgcgac tgattgtttt ttcttcaaat tgctcattgt
     2161 tcagcatatc ttctcctgct ttctttgtaa tcataactgc ttttttttca tttttcaatt
     2221 tttctcaaaa actccttatt agtttaccat agataaacct tagattttct ctaaatatag
     2281 aaaatccttc tttagtccga tactctttct agtagtccgt atgctaaaat aagtatgtaa
     2341 tcgaataacg aaaagaattc aatactcaac gataaatgac atggagggtt tattatgata
     2401 aaacgaaatt ggccgtggct tctagcattg gtcttgctat ttctctttgt ccgaaacaat
     2461 agtttttctt ttgttctagg acttttattg ataggtgtag ttttagtttt gtggggactc
     2521 tttggttcgc gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac tgacgaactt
     2581 gaaactcact atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca
     2641 atgaatcaaa caaaaaatga gatcaatcaa ttgcaaatga atatgcagca gacagccaaa
     2701 cttaaagcaa tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa
     2761 ttggtgaaag aaccaaaccg actgcatgaa gcaagtcagt ttctttatac gcatctgccg
     2821 aatctagttg atttgacaaa taagtataat gagatcaatg accatgagat caaaaacaaa
     2881 cagacctatg aaaaactaga agaaagcgcg cagatcatcg accagctgtc agctttaatc
     2941 gcacaagatt atcaaaaatt cgttgctgag gatctagatg atatcgacgt agagatatct
     3001 gttgcgaaac agagcttaaa gcgtgataat aaatatgaac cagaacaaaa ggattaactc
     3061 ttttgttcat aaaagaagac tcggaacatt cagatgatgt ctggctgttt cgagtacttt
     3121 ttctaaagta gagacattca tgtaagaatc gagaacagat tcaacaagga ggaatttttt
     3181 atggatcata aaccgactga aacaaaagat gttacaccag ttaacgatac acttgaagat
     3241 ttattgaaca acccgttttc aactccaaca gatacgctga ccacttcaca acaagcggag
     3301 attgatcagc tccaagagca acagacagct gctcgtttaa ttgacaaact accggcggaa
     3361 cgccaagaac aagctaaaca gctagcagca aaaatcgatg ctaatgatgc acagtctgtc
     3421 atcagctacg gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat
     3481 catgtgcaag cacaagatat tggtcctgta ggagattcct taactgaact gatgtatcgt
     3541 ctccaagaag ccaatcctga cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc
     3601 ttcgggaagg tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa gattggtgca
     3661 caaattgaca agatttcagt gaaattagat aaagaaaaag acggtctgct aaaagataat
     3721 ctgatgttag aacagcttta ccagaaaaac aaagactact ttgatgcact gaatatttat
     3781 atcgctgctg gagaattgaa aatggaagaa cttcaaacaa cgatcattcc agaagcgatg
     3841 aaaagagcag aagaaactgg cgatcagatg gatgttcaga ttgccaatga ttacacgcaa
     3901 ttcctagatc gtctggataa acgtacgcat gatcttcgct tagctagaca gatcacgatc
     3961 cagcaagcac cacaaatccg tttgatccaa aacacaaatc aggccttggc tgaaaagatc
     4021 caagcatcta tcgctacagc cattccgctg tggaaaaacc aagtcgtgat cgcattgacc
     4081 ttgcttcgtc aaaaagatgc agtaactgct caacgccaag tctctgaaac aacaaacgac
     4141 ttattgaaga aaaattcaga aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat
     4201 gaacgtggaa tcgtggatat cgagaccctt caaaccactc aaaatgattt gatcgaaaca
     4261 atccaagaga cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta
     4321 gagcttggac acatggaaga agatctgaaa caaaaactgt tggatttaac ccaatagatc
     4381 acctttcgct tgttactaaa acaaacgccc aacaacagtt tattacaact gttgatgggc
     4441 gtttttctta tctttcttct aacttctcca ttggagtcta tttctcattc atcttgctgt
     4501 gtccatagcc atagccaaaa taaatgatca gtccaataac cattacgata ccgaacacga
     4561 tccatgtcac agtttgcaga cgaagcatca atacaataca agtcaagatg gagacgattg
     4621 gcaatagcgg aacgaatggc actttgaatt ctccagcttt tggtttgcca aacatttttc
     4681 tcaacttcag taagcctaga ctaacaagca tcaaataggc gagcgccatg atattcgtta
     4741 gttccgctaa tacattcaat gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg
     4801 cataagtcgc ttttttcggt gtacgatgct tttgatcgat ttctgtcata aacttaggaa
     4861 gtaagccatc cttgctgaca gcaaacagta aacgagcaag tgaatacatc atcgagatcg
     4921 ttaccgtcag tagcgttaaa attgccccta cagcgatcac cgcgcccact gttccatgtc
     4981 ctacatacct cattgcaaaa gccactgggt cattgactcc tagttcagta tacggaacga
     5041 ttcctgtcaa gatcagcgtc acgatgatgt aaaggacagt agcaatcagt atcgaaccaa
     5101 tgattcctct tggcacatct ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg
     5161 catcaaatcc caagaaagca aaaaacacaa gagctgcacc atctagcaca ccggaaaaac
     5221 caaaaggcat gaatggctgc cagttatcag gtttaacgaa aaatattcct acaatgataa
     5281 acaacgcgat gataccaaat ttgacatata ccatggcatt attcaaacgt aatgctttct
     5341 tggcttcaag cgacacgatg taagtgacga atatcactac gagcaccgca atcaagtcaa
     5401 tgtatgtacc attttccgga ttgtacccgc cagttaatgc ttgtggaagt tcggccccta
     5461 aacttttcaa aaaaccttgc atatagccag accatccgga agcaacagag gaaaccgcta
     5521 gtaaaaattc cccgatcaac aaccaccctg tcagccaggc agcaaattca ccaaatacaa
     5581 catacagata ggcatatggc ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac
     5641 ttagtccaga caaaatgaca acgattgcag ctaagacaaa tgaaagtgac agtgctggac
     5701 cagcattttg attggccgca acccctgtca ccacaaatat tcccgtaccg atgatcgcac
     5761 caattccaag catgatcaaa tcagacgttt ttagttcttt tttcatctca ctcgtatgat
     5821 tgagattgat ctctttctta cgaaacaaat ccattgaagt tcctccccat tttaggttgg
     5881 aaaacagcga ttaaactgtt ttcatatacc ctaaaaggat accacctaaa atgactaaaa
     5941 ggcaaccaac aataacataa atgagctctt ttttcgtttt tctttcgcca aggatgaaaa
     6001 tcccacctaa agtagagata atgatcccca tttgtgaaag tgagaagcca acagctaaac
     6061 caagtgattt gactgttaga agcatgcaga tgtttcctaa gccccataat aatccacaga
     6121 tcatgttttt ccacacaaac tgatcgactt taactttacg gaaagcaaag aagcttgcgc
     6181 ctaagatcat tccgatactt tgaggtaaga taattgacat cgcatccaaa ttcgcccagt
     6241 tgacgatgat ggtatataca ccataaccaa tcgtagagaa gatcaatgct ctaaaaccct
     6301 taccaaaatc aagcatttta ttatctgtat ctatttttcc ttcgtcatct tgtcgggcag
     6361 tcaaatacgc accgcttaca agaagagcta aagcaataat accgtataca taatctttcg
     6421 tttgtgtcca ttcatggaag aaaacggctc ccgcaatcgt attcaaaatt aattgaaaac
     6481 cagtagacaa tggtagccct acagaaacac ccatgtactt cattccatgg aactgtccat
     6541 tttgtccaac actccaagct aagccggata aaaatccgat caagaaaatc caaggggtga
     6601 ttacaggact aacaacgaaa aagacaacta aagaaaacag aaatgcaccg atcgtcattc
     6661 ctagtgtttg ttgattggca tcgccaccaa ttttaccact tactaaacca atactacccc
     6721 atgccaacat agggactaat gctattaaaa tttccataac caatctcctt ttatttcaca
     6781 tgataagaaa cttatcagcg aaaacgttaa caacacaagc actttttttc gtttctaacg
     6841 attttctaac gtttttcatt ataaaaattt aagattggga aaaataaagt cattgttgtc
     6901 ccttttcctt cagttgattc agccgtcaaa tggataccta atttttcggc cagatttttt
     6961 gctaaataaa gtcctaatcc tgttgaatgt gttttgctaa gtcgaccatt tctcccagta
     7021 aatcccttat caaagatccg tttcaagtct tccgctggga taccgatccc tgaatcttta
     7081 actgaaaggc tgactccttg cgcttccttt tctatgctca caatgatgtt tccgtgatcc
     7141 ggcgtatatt tgatggcatt actaagcaat tggttgaaga taaaagcgat ccatttagca
     7201 tcggtcaaaa cagtatgatc ctcaccaacg atctcgtagc gaatattttt ttggataaaa
     7261 taatttgcct gactgcgtat cacaggctga atgattcctt ttagtccata ttcttggatg
     7321 aggtagtccc gcgagaaact gtcgagtctt gcataataca aaacttgttc tacgtattca
     7381 tcgatttttg ataattcatt ttctagtaat gtatactttt gatcgtcaat atcgaactcg
     7441 atcgaacgaa ctagcaattt agaagcggaa agtggcactt tgatttcatg gacccatgag
     7501 tcaatataat ctttttggtc ttgttgatta ctgattgctg attgcattac ttcttggtgt
     7561 tcacggatca gctgattgat atattcttct accagttttt cttcttctgt cctcccacca
     7621 ctcaaataat tttgcaaagg ggaagttttt tgtatgtcgg ctaattttcc ccaccatctt
     7681 ctcttgctca agtaaccacc cagcagataa aaaatcagaa agacgccctc aatcaaagct
     7741 aaataagcaa ttgtgctcaa atcaactttg atgtttggcg ccagccacat cacaaagatc
     7801 gtcaatgcaa taaaaaacag ccatcccaag atcaatgtcc actgatcttt caaatattta
     7861 aaaaaattca tcttaaccgc tcatcctttc agggaacgat atatccctgt cctacttttg
     7921 tttcgatata tccttggata cctgcctgtt cgatttttcg gcgtaaccga ttgatgttga
     7981 ctgtcaatgt attgtcatct acaaatcgtt cgtcatccca cagtgctcgc agcaattttt
     8041 cccgacttaa gatctttcca ttgtgcttca tcaaaataaa taatagacga tattcatttt
     8101 tgctcaaatc aatgatctct ccgttgattt ccatacttcc gttgtcaaca ttcaacgtaa
     8161 tgccattgtg gctcatcatt tcactagata attctgtgta gttgtaagaa cggcgaagta
     8221 atgcattgat ttttgcaatc agtacatcga tttggaaatg ttttgtcaca aaatcatctg
     8281 cccccatgtt catagccatg atcatatcca tattcgtatt cctgctggag ataaaaatga
     8341 tcggtacttt cgatacttca cgtatttttt gacaccaata atagccatcc aaaacgggaa
     8401 gattaatatc tagtaataca agctgtggtt cttctctttc aaagtctgag aacacttgat
     8461 tgaaatcggt cgttccaaag gtttcaaatt gccatttctc aagttcctcc atgatcaatt
     8521 gtcgaatgat ctcttcgtct tcgactacca tgatttttgc cataaaaaat ccctccatct
     8581 ttcttcttgc tactacaata tcaaagaagc ccttgtgaaa caagttggct tacctggaaa
     8641 atgtcatttt agcaaacgtt aaaaaaaact ggaaatatgc gatttaatcg cgtttgtctc
     8701 ttccaagcag cttaaattta ttatgttatt cttaataatt ggtctattat ctgttatttt
     8761 tctttcgttg tttttgtctt ttaaattttc gataacacca ataaccgaaa agcgttagca
     8821 ctagcaatat acctagaagg agtataagta atcgataaag atggtatttt tttgtctgtt
     8881 ctatctttga agagacttct tctgtaaagg gaactcgtct tcctgtcact agtaatcggt
     8941 gagtgttcac ggtgtaaggt gtacaagtga taagtgtgac tagatcctgc ttttctctaa
     9001 ttgctaatgt atttgttttt gttggaagga caacaatttt ctcaacgact tcataagcga
     9061 gcatccgacc atacacttca agatagaatc ggtcatcttg ctccatttct tcaagatccg
     9121 taaagagttt tttctcagca ataccgctat gggcggtaat cacggaatgt gtactctctc
     9181 ctccagttgg aaaagaggtg ccttgcagga ctgtcgctcc tctatctaac aaaagatcat
     9241 tcgtttcatc aaataaaggc aagctaacat tgattttcgg aatatagatg gctccgatca
     9301 tatgttgttc gtaatattct tttcctggat tttggacatt tctaaccgct tgctcaaaag
     9361 gatctttgac ttgtcccatt cctgggatta ctgatgtatg agcaagcgtc tgatttttct
     9421 tttcatgcac tgctaattgt tgctcatgtt tttcttcttt atcgcgttca agctgctttt
     9481 gataattctc gatccttctt tgatccaaat aattgctgag agcatctgct aaaaatggat
     9541 atgtaaaaac tgcagctccg gcaagaaaca tcactgccat aaataattgt aataaaagac
     9601 tgatttgggt ttgaggtcta ttacctattt tctttttcat tttcttcacc ttcttattta
     9661 aaaaaactgc agcaaccttt ataaaaccga ttgctgcagc ttatttttgt tgcttatact
     9721 tcagacttca ttttagattt acggtaccag ctgtacgctc caatcatcaa acttattccg
     9781 attagtaaga atgccaagag cccgttccca ccagtagcag gaagtgaccc gcgtttcata
     9841 ttcggtactc tttctggccc tggattttct actcctacga tctgaatttc ttcattatag
     9901 cttccataag agatgataaa ttcagtaaag gctgcctcac ctgttggaag tacataacct
     9961 tctggcgcca tggtctcttt catttgatag gttccataat ctaacccttg aacctcaaag
    10021 tatccatttc catctgcgac aaattctgtt gcttgtccgc ttgttgccca ctcaattcta
    10081 tctgctcctg attcataaac agctaaaggc gactcccctt tataaaatat tgcgtattca
    10141 gaacggtctt cattccacaa ttcgaatctt gcatctttaa gcaattcact gcttgatgca
    10201 tcatattttt caaactttct tccacctgtt gtgactttct ctggcggctc tactccttct
    10261 tcataatcaa attcatcatt tcgtccgatt tcaataaccg ctgtgttgtt atggaaatca
    10321 tccgcttcta gttctgtgga aaccttcaaa ttatacgtga tcgttaaatc tcctccacgt
    10381 gcatctgcta atctacctaa agtatctact ttggctactc cattttcttc tgtcaaaatc
    10441 gtcacgacaa acccgttgct tactacttct actatataat cttgtccttt tgttaaaatc
    10501 gtctcgccgg ctcgcacttc tatttcctga tcataataag ttagaccaac tgtaggcgta
    10561 tctgtgattt tgtaattttt gataacagcg cttccgtttt caaggacaga atcgatgaaa
    10621 taaggaatag gtgctgtgat cgtgtaagaa gccgtttttc ctctttctag atcaatataa
    10681 gaaatcgttt cttgttctcc agcttcatta ataatcgtta cttgtctaag gtcgctttcc
    10741 tctacattca tctgtttatc tgcttctctg atttcgtttt tcggatacaa atgcacatct
    10801 gtattgtatg tgtctttcca tgtagaacca tcccacacgc catccaccat atcaggcatc
    10861 atcactggca tcataacaac cattggagac gcgattctgg aaatatgagc tggcgaataa
    10921 gtttctaaga aaagatagac tttatctcta gaagaaggtg cttcttccgt cacttggacg
    10981 gtagagaata ccgcttctcc agcttcattc gttgtttgtt tgtcaatact tgtcagtcga
    11041 tattttaaaa accaactttc tgtgtgccgt ttttgaatcc attcaatcgc ttcggcagaa
    11101 ttaagaccgt cttctcgttc cggtgttaaa ggattatctc gaatcaaatc gtaatatact
    11161 tccgttacgt caaacaaatc aaaatcgact cctggaatat tttcttcacc aaaatccgta
    11221 gaaaccaatc cgctattcgg tcgttcttcc ggaacagatg agaaaccttt tttgtgtaat
    11281 gtaatcgtca cttcacttgg tttttgtgcg acttcttcgg ctttgactag ttttccgttc
    11341 ccgaaaatcc ctattcccgc agtaacgagt aacagtacac tcattcccgt gacaatcatt
    11401 tttttattca tttttttctc cccgttcttt ttttattaat aaaacagcac ctaacaataa
    11461 aagaattcct agtaaagaaa tcaacgttct tgcttcattc gtttgcggga atacggacca
    11521 ttttcctgat tgacctggat tgcttggacc actcggctct ttcggtttgt ccgttgtttc
    11581 ttttacggtc tcgttataaa cacgtggtgt tgcggattca taggcagaga ctggaacttt
    11641 cccggattct aattcagcca tctcctgtcc gttaactact acatattgtg gttggccttt
    11701 tgcatccacc caaaattcag ggatgatgac ctcgattctc ttacttgctt catccatctc
    11761 gtaaccttcc acacctttta attcttcaaa ataataggtg ccagaaggca agaaacgttg
    11821 tcctgtcgta acaaggccct ctttatccga aatgaatttg cttacatttt tgtcattcaa
    11881 aggatcattg tctgctggtt tgatccattg atttttcaag tcattagcag gagccatatc
    11941 taaataatat ttcccccctt ctatcatctg atatagagca aaaacagcac cttctaatgg
    12001 ttttcctttt tcagttgttc ctctttgacg tccatacttg aagaagtatg gatctcttag
    12061 ataaccgaca tttttaggat agatatgaat ctcttgcagt tcctcggttg attctgtcgg
    12121 gtgataaatt ggcaaaatag cagcaattgg gatagctgtc tgatcgatat caacatccaa
    12181 tccaacttct ggatcaatcg attcttcgaa aatcaaataa actgcatccc gatcttggtt
    12241 tttcttaggt gctttgatac gtgcaatccc gtctcctaat ccgccaaccg tatctttccc
    12301 agttgttacc gaagtcccta tttggacaag cctttcttct gtgattagct gacggatatt
    12361 gtttctgttc gtttcagcga tttgctggac gaattcttta ggagtcatgt tttcagcagc
    12421 ttttttttca tgataataat cggtcatgtc gtaaatggca aatgtcgctc catttagtgg
    12481 caccgtctgt gaaatgatat ctgctgtttc ccctgcttcc tcagagatag ccaagccatc
    12541 attttcatgg tattcaaatt gatcgttata atcgatatct cgaatcatcc gtttatgcaa
    12601 cacgaattcc acgacatctt cccctgattc ttctgccgac atcgcatgtg gaagaaggac
    12661 tgctgcaagc ggtagtaaaa caagtaattg aagcatcaaa cacaacagct ttttcattac
    12721 cttgctccct cctttttaga ttgtctgaca aacaaaccat aggctgctcc tgcaagcgcc
    12781 atgaagaatg cagcaagcag gatatagaat aatcgtccgg atcctcctgt agcaggcaac
    12841 ggattttcag gatcgttccc gacactaagc tgtaatctga ttcgatttgt ctccaaaagt
    12901 tcatattcta actcttgctg tagatcaggc gtctctgctt cttcgtttgg ataatacgaa
    12961 atctcaactt ttagtgtacc cgcttctttt cctggaacta cccgatcatt ttctatgtca
    13021 ggttcttctc gataactaat ttctaaaata aagtaacctg gaagttttct atagctgtca
    13081 ggtgacttca cttccttgat ccgatagacc ccttccaaca agtctgaagc attgaatagg
    13141 gaagaatcgt ttggatctgg tgccaagcct ccctcgatga tttcatactc tcctgcacta
    13201 ttttgtttct caattgaaaa ttctgcgcct tcaagacgtc ggctattcat actgtcaatt
    13261 ttcaaaagtt ccaaatcgat tggcgctagt tcatcttcta aggttacttg tagtacacca
    13321 tcgatgattt catagccttc cggcaaggga gcagtttcgt cttcgatgac cgtatcttca
    13381 atgataaact taccttcttc attcagtgca aactggaaca cttgatcaga tagccgatac
    13441 ccatttggtg cgacagattc cgagacctca tattcaccaa tagacagatg cagaggttgg
    13501 tcgtaattta aatcgacata cttttctact cctacggcac gagaatagaa gtatccgatc
    13561 ccaggcaaac gtttcgattc ttctggttct aacggattac cagtgtatgc tcgatacagc
    13621 gggaaggaag aatcccccac tttcctgata tcgaaacctg caccatctaa tgtatggcct
    13681 tcatggtcta attttctaac tcggatctcc cgataatcaa acgtattgta agcagtgaat
    13741 gctacccata aacgatcctc ttcatctgtt gtatacgtga gatattgcca attttcttca
    13801 cctgtttgct tgagttggat agtccctata gcattatcgt aaagtcgcaa gatccatgtg
    13861 tcattttcat caggttcttt gaaaccttct gggttctggc tctcaatgat tttgtattct
    13921 gcatagttgg tagtttcttc actatcctta taaacaaatg aatctggctc tccgattgga
    13981 tgaataactt tctcaccatc cacgattaca tatttcccgt cttcatctac tgaatagaac
    14041 gtgagtctcc ctttctcttc ctctacttcg ttcacaccag aaagcccttt cgctactgga
    14101 tatccgattt ccagtccgtt accatctgtt tcataaagtt caaactctgc gccattgatt
    14161 ggcatattac tgccttcaat tagtttattg atattcatgt ggattggctt gaattcattt
    14221 cgcatttcca gtttgacata caactcatca tactctgtgc cttctatgtt aggctcttgt
    14281 acaacagaaa gcttgtgata ttctccttca taataggaat tcggatcttt accttccggt
    14341 ccgccgactc tcgaaccaga tgtatattca tctacaagct cgtagtgacc taacatctca
    14401 tcttcgttgg ctgcatattt tttgatttgg aattgatacg tctcatctac taactgataa
    14461 cgtggtggtc cagcgatctc tctgatccaa aatatgtcgc tgactgctcc agtttcatct
    14521 cctaaaatcg gcatcttcgt ttcaaagatc gctattccgt ccgtggtagt tttacttgtt
    14581 tcgattggtt cggcttcccc actctctgga ttttggtcat ataatgcaaa ttcgactcca
    14641 gccaacggct cgccattttg acccttcttg ctgacttgga aattacggcc tttatgttcg
    14701 taacgaatgg aagaagatgg gaaatacatc cgtccagtag tgatatcatt ctcataatcc
    14761 tccaggttct gaatataggc catattgttc actgtgcaaa aatttccgtc ttgagctccc
    14821 gcatcaagtt ttacttggta cttgatcctg accatttccc cttcacctaa agaaatcgga
    14881 gaaaatttat aagcttggtt ttcccgtccg acatacgttg tcggagtcgc cactacttct
    14941 ggtggggctt gtccatgacg ttcttcatcc catatttctg gctcttggta cttcatgccg
    15001 ttttctcctg gatctagatg gtaaaactga atatctgccg atctgccagc tccaaatggt
    15061 gagacattaa aaccagtttc atcataaagt gtggcttgct ggatcgttct cttgaaggta
    15121 gaagagatat cctccaaggc tgtaacaatg tcattcttgt tgttcgcatt gtaatatcgc
    15181 ggtgttacgc catcttcttc taaatcagca atatttttca atacatttcg tccaatagca
    15241 tctagtcgtt gtctcccgct aacgtttcca tcgattccta aaccaatgga gaagatactt
    15301 ggcgggttct cttgatactt atttttcagc caatgatgat aagcaactgt gttgatcgca
    15361 gatgactttt cttgcgaagt aggactgcca ttcgtattgt tattagaaga accatcacca
    15421 acccagtgag tcgctttatt atctccttct tttacctctc cataacgata gtgtacattt
    15481 acttcatctt ctggacggtt aaagtcagat gcataggtaa ctggatgagt aaaccctgca
    15541 ttagaagtat tcccagaaaa ctcctcaaaa tttctgaaca aaacgtcatt atcttcagca
    15601 atcttatctt ccatcacagt ccagttgctc caagctccat tagtgctcgt acgataactt
    15661 gactgaatcg gtgcataact gaaggtcggt gtcccatctc ccacaacgat taggattttt
    15721 tctggatttc tattttctcc gccattgtct ttatacaatg tttcatagcc atttttaagc
    15781 cctagtgtaa gcggagttcc tccgattggt gtacgattta ttgctgtatt ctcacttccc
    15841 gtcaaacgat tgatatcatt tgtcaatgga aagatgtccg aactgatttg ctcatggtta
    15901 ttgatagctg tactattatg gtaaaaattc accataccga ttcgaatatc cagactcggg
    15961 tcagagagca gatctttact catttcgttt actgcctcaa tcaacgcagc atttttagtt
    16021 tgactattgg tcccagcggt taattcactc atactcgctg acttatctag aacaaaaacg
    16081 atatcgattg gactgatttc ctttttctcg cccccgatga catctagata gacattgtaa
    16141 agggttgttg catcttcagg atgtagatca gatgctgtgc tgcttggcag aacaggttct
    16201 acccattttt tgatgtacgc cccgttataa tccaagtaac tgttttcaaa attccgttca
    16261 tttccccaaa gctgaacggt ttgagctgga acgtttctat cttcttcgcc tgcagttgat
    16321 ccattattag tagcatagtc gtaatttcgc cagttcgctc ccccgtttgt ataatcacta
    16381 tcatcgatca agttctctcc gtcatatggc gagcctttgt ctccttgata gcgttcattg
    16441 ctttgtgtcc aatttcctaa cacttttgta tagtcattcg ttgtaaactt cgggtagcga
    16501 tgctgttcgt tttcaacttc tccgctagga tagatatatt cataggggtc ttgcgtagct
    16561 gttggcccaa gacgctccaa aataccgttg aataactggc gttgaatgcg ctggagtgct
    16621 tgaccgctgg gtacgattgg ttcgtccagc ttttccatct cattttcttc gctacgttga
    16681 atttcagcgg aatcttctgt atcagtttca ttttcttgta attcttggtt atctattctt
    16741 tcatcaacca ccacttgaag aggtagttac ccttcgttca catcggggat cttttctgtt
    16801 tcaaaaatca atgtgccggt ttctgcttct tttgaataaa cttttccgac ataccattct
    16861 tgaccatcga tcgtctcgac ttttaggtct tctccatctt catcatagga ggatagacct
    16921 gttccacgaa cgttttttac ggtaccaatc ccggcggctg ccgcatcgat tgctaaacgt
    16981 attctacctt cattggcact ttcctttttt tcaaaagcca gatgccattc aagggtttct
    17041 tctttttcaa tatacttccc ttcaattttt gcatagtcct catctaagaa tacctcatcc
    17101 tcctttgttt ctgttgactc agcagctaca tccagggtcg tcatatgata gactgactgg
    17161 aaatatggtc caaatagcaa actgacagtt actaactgca aaagcttcaa ttttaaccct
    17221 ttcacatcct tatactcctt tcaaaaatat ttgtcttctt tcacaacaat caacaaaaca
    17281 atgcccatct aattataatt taatttaatt ttaattttaa atcaaatgat atccttttta
    17341 gttaaaaaa
//
LOCUS       DAGCYL010000042        17128 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.043, whole genome shotgun sequence.
ACCESSION   DAGCYL010000042 DAGCYL010000000
VERSION     DAGCYL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.043"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            48..443
                     /locus_tag="MB216_001086"
     CDS             48..443
                     /locus_tag="MB216_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525938.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            768..1385
                     /locus_tag="MB216_001087"
     CDS             768..1385
                     /locus_tag="MB216_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4525939.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1426..1725
                     /locus_tag="MB216_001088"
     CDS             1426..1725
                     /locus_tag="MB216_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBT4525940.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1759..1926
                     /locus_tag="MB216_001089"
     CDS             1759..1926
                     /locus_tag="MB216_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4525941.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1971..2534)
                     /locus_tag="MB216_001090"
     CDS             complement(1971..2534)
                     /locus_tag="MB216_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4525942.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(2562..3242)
                     /locus_tag="MB216_001091"
     CDS             complement(2562..3242)
                     /locus_tag="MB216_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HBT4525943.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGITA"
     gene            3317..3406
                     /locus_tag="MB216_001092"
                     /pseudo
     CDS             3317..3406
                     /locus_tag="MB216_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            3483..3812
                     /locus_tag="MB216_001093"
     CDS             3483..3812
                     /locus_tag="MB216_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBT4525944.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            3802..4089
                     /locus_tag="MB216_001094"
     CDS             3802..4089
                     /locus_tag="MB216_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBT4525945.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            4375..5232
                     /locus_tag="MB216_001095"
     CDS             4375..5232
                     /locus_tag="MB216_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBT4525946.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            5233..5781
                     /locus_tag="MB216_001096"
     CDS             5233..5781
                     /locus_tag="MB216_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525947.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(6078..7025)
                     /locus_tag="MB216_001097"
     CDS             complement(6078..7025)
                     /locus_tag="MB216_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBT4525948.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            7213..8400
                     /locus_tag="MB216_001098"
     CDS             7213..8400
                     /locus_tag="MB216_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBT4525949.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            8381..9685
                     /locus_tag="MB216_001099"
     CDS             8381..9685
                     /locus_tag="MB216_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBT4525950.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            9711..10724
                     /gene="citC"
                     /locus_tag="MB216_001100"
     CDS             9711..10724
                     /gene="citC"
                     /locus_tag="MB216_001100"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBT4525951.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            10728..11021
                     /gene="citD"
                     /locus_tag="MB216_001101"
     CDS             10728..11021
                     /gene="citD"
                     /locus_tag="MB216_001101"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBT4525952.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            11026..11931
                     /gene="citE"
                     /locus_tag="MB216_001102"
     CDS             11026..11931
                     /gene="citE"
                     /locus_tag="MB216_001102"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBT4525953.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            11924..13483
                     /gene="citF"
                     /locus_tag="MB216_001103"
     CDS             11924..13483
                     /gene="citF"
                     /locus_tag="MB216_001103"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBT4525954.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            13480..14037
                     /gene="citX"
                     /locus_tag="MB216_001104"
     CDS             13480..14037
                     /gene="citX"
                     /locus_tag="MB216_001104"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBT4525955.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            14030..14893
                     /gene="citG"
                     /locus_tag="MB216_001105"
     CDS             14030..14893
                     /gene="citG"
                     /locus_tag="MB216_001105"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBT4525956.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            15127..15536
                     /gene="tnpA"
                     /locus_tag="MB216_001106"
                     /pseudo
     CDS             15127..15536
                     /gene="tnpA"
                     /locus_tag="MB216_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(16173..>17128)
                     /locus_tag="MB216_001107"
     CDS             complement(16173..>17128)
                     /locus_tag="MB216_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBT4525957.1"
                     /translation="GTLELKVPRTRDGHFSPTVFERYQRNEKALIASMLEMYVSGVST
                     RKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVRE
                     ENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEYLKERGLQGTELVISD
                     AHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAR
                     EAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         5941 a         2652 c         3326 g         5209 t
ORIGIN      
        1 ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg atgattatgg
       61 aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga tgtggaatta
      121 tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc aagatagtag
      181 attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag tttgaaagag
      241 taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa gagtctccaa
      301 atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa aagaaagaat
      361 acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct aaattagaaa
      421 tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa agaagtcaaa
      481 ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg gataactagt
      541 ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg aataaatata
      601 atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat gccttgattt
      661 tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt ctgatttaat
      721 ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg agaaaatttt
      781 tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt tccgcgaagg
      841 aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa gattttaaac
      901 gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc tatctacaat
      961 cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga gaattaacta
     1021 aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact gctagttaca
     1081 agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta ctcagctata
     1141 tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta gaagctgcga
     1201 aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa tttcctgcga
     1261 tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat caattgggca
     1321 tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg caccactttt
     1381 cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga cttaacgaat
     1441 caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc catcggtttg
     1501 gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa tttcctggtt
     1561 tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa ggaacaaacc
     1621 gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt cgaattttat
     1681 gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt agtaaaattt
     1741 acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat tgaaataact
     1801 agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt agcaaaaagt
     1861 gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg ggtacatgga
     1921 acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt ctacaaatca
     1981 ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt tgcaatctct
     2041 gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa gcgtttttgc
     2101 ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc ggctttttga
     2161 gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt ttgtgcaata
     2221 taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc atttccagta
     2281 ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg tttcataaaa
     2341 gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga tgtaacgatg
     2401 acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg ctcagtatct
     2461 ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttcggt tctgttgcga
     2521 agtttgaaaa tcaaacgcgc cctctctgaa ctccaaatat cttaggctgt tattcccatt
     2581 aataccttga tttcagtaga caccgaaaag ccgaagagcg ttccatttct tcggttcttt
     2641 ttatatattc ctcgaatggt ctccatgccc ttaatcgtgg aagaggctgt acggagactt
     2701 tgataaaatt tatttcgtcg tttaataggt cgatggtctt gttctattaa attgttaaga
     2761 tacttcacag ttcggtgctc tgtcttagta tataaaccca cactctgtaa ctttctaaag
     2821 gcggagccaa gagaaggtgc tttatcggtc acaattgctt tcggctcacc aaactgttta
     2881 tggagtcgtt ttaagaaagc ataggctgct tgcgtatccc gtttctttcg taaccagata
     2941 tctaaggtta agccgtccgc atcaattgca cgataaagat aatgccaacg tcccttaatt
     3001 ttgatatagg tttcgtccat tttccatgaa tagaaggatt gtctattttt cttcttccaa
     3061 agataataga ggactttgct gtactcttgc acccaacgat aaatcgtagt atgacaaaca
     3121 tttattccac gatcatataa caattcctga acttcacgat agcttagatt gtaacgcagg
     3181 tagtaaccaa cagcgacaat aatgacgtct tttttgaatt gtttgccttt aaaatgattc
     3241 attactctgt cctctctgtc ttttttctca attttacact aaaatagatt ttttggaaaa
     3301 ctttgcaaca gaaccgaata gacaacaccg ggttgtttat aaggtagatg aagatgaaca
     3361 cgtggttgaa atttattcag caaggacgca ttacgagtca cagtaattac ttgtaaatac
     3421 attatctcgt ggtataatgt atttactaag taattacata ctgttagaaa tgagggattt
     3481 aaatgattac aaaaacaaga aaacaaggca attcaatcat gttgacagta cctaaagatt
     3541 ttaatgttcc taatggagtc gaagttgaag caaagttggt agagaatggt attctttatg
     3601 aatttgttga accacaaaaa gaattttatg attttagcga agatattttg tctgatataa
     3661 ttgcagaagg ctacgataaa gatgagattc ttgtagaatt taagaataga aaaaacaaaa
     3721 tgcattcttc ctttagagat attgctgaag atacacttac taattctaaa gtaatgacta
     3781 aagaggagtt ggcaacagaa attggcttat gaaatcgttc cttcaaaaca tgtcattaag
     3841 tatttaaaaa agctaaaaga gaagccattg aaagaaaaat ttttgaattt aatttatgat
     3901 gaaattgcga ttgatccata taagggtgaa caaaaaactg gggatttatc aggtatttgg
     3961 tctagtgggt tcaaatatgc gggcacaaca tatagagtag cttacgagat tatagaaaac
     4021 aaggttattc cagtattgtt atgtggaacg catgaaaact tttatgaaca gttgaaaaaa
     4081 ttaatctagt gatttgttgt cgttcttaag aaggacggct tcttttttat cgaaaatctg
     4141 attcgtctac atgactaaag tcgcgagtat tctctgcttc tcgataaatt aaataacgtc
     4201 attactttga ggaaagctat aaatcaatat atttcaactt ttccttattt atgtactacc
     4261 ttttacttat taattataag gtagtacata tatatctttt ttttggtagt aactaaaaat
     4321 aaatatgtta taatagtatc aaatgaatat tgctaaaaga aaagaggtag gaacgtggta
     4381 gcgaagaaat atgtaattgg taattttaaa ggtggcgtag gtaaaagtac gtgcgcgcaa
     4441 atgttagggt ttgaatcagc agttaatgaa ggaaaaagaa ccttgataat tgacttggat
     4501 atgcaaggaa atacttctga tgtgatgaac ttaacgcata tgaacttcac taacgaagaa
     4561 ggaggcggag aaggtgaacc gattgtttac gaaaatactg tgactgacgt gttgataaac
     4621 aatcttccac ctaaagaggc tgtctataaa gtaatcaata acctctatat acttccagca
     4681 gatatgtcct ttgaaatgta tgatgattgg atcaaagacc aatataatga ttcattatcc
     4741 caattcaaat atatgaaaga aaagctagaa ccattatttg atgagtttga cgtcatctat
     4801 ttagatgtcc caccttctat ttctgtatat agtaaatccg cgatgtacat tgcggactgg
     4861 gcaatcgtcg tcttacagac ccaagtaaaa tcgatgagaa atgcgatgca atatcttgag
     4921 tatatggact tttttgtgaa agaatttgat acaaatttat atgttgctgg tgttattcct
     4981 ttcatgttgg aaagcggtga cgcagtagat caggaaatgt ataaacaagc ccaagagatt
     5041 tatggggaac atttgattaa aaatgttgta ctcaaaaatg cgcgcttaaa acgatacgac
     5101 ggatcaggta ttacgatgga gaagacaaag aaaggtctct taaaacaatg ggatcgcaaa
     5161 agccacgaat tatttatcag tattttgaaa gaattggaag aacacgagaa ttggtatgcg
     5221 gaggtggagt aattggctga catcaaagga cttgatattt ttgggaacaa caaaggactc
     5281 gccaaaataa aaaaagaagc agaagcaaag aaaaagcagc aagcaattac accatctgaa
     5341 aataagcaat tagaagtgca ccaagttcaa gaaataaaaa cttcctccaa tgttgaaaga
     5401 cagagtagag gaagaacaaa aaaagttggc gcaccaatca gaaagtttga taaggtttat
     5461 tccaccaagc atcctttaaa actgtcgact ttgttaaatg cgacttccag agttttagta
     5521 gaaaagtatg agactaatat gacaagagat gaattgctta gaaaagcttt agatgagtat
     5581 attaaacaga atcttacgca agaagataag caagatttgt ttaatgacgt ggtgaaagaa
     5641 ctagacttgt tcagagaaaa atatccaatt gttccagaat tagatgaccg tggagaaata
     5701 ataagaacta ccagtcagat aaaacaagat accgaagact cactgcgcaa agggtgggga
     5761 atgaataata aagttagata gaaccccctt tttatagcat aaaaaagatt tagaaggata
     5821 tatgatccta ttgcatgaga agtacaataa aattaataag aagtaacatt aacgaaaaaa
     5881 cgtggaaatt attcagtgaa tacaaagaaa ttaaatcttg aaaagtaatg gagtatggct
     5941 tggaaaagtt caaaaaatat caagacaagc ttctcaaaaa aagggatttt gatatccaat
     6001 aaaaagagtg aatcattgta ctgccctcca aaaaagtagt accaaaaatc taaatttttt
     6061 ggaggatttt acatgactca ctcttttttt atcaaagaaa gtattttcca agcagttttt
     6121 tcatccaaaa ataaatctgt aaaaaaatta ccattcaagg cgcctaaaca actctccgct
     6181 ttgaacttac ttttacaaat accaattcgt tttggaatat cgaaaatttc ttttaaagaa
     6241 agaccaaaag tcttatcctc agatgctttg atgaattcac cattgatatt gaagggtctt
     6301 ccgtaaagaa atccaacagt atccgtcatg cttggaaata ggattttacg atgcttgttc
     6361 cagggttcga cagaatcaat agaggataag gtgccaattc cagaaattaa catatctgca
     6421 tgcgaagcgg ttgctaaggt attagctaaa gaaggttcta gagccattag ttcacgtgca
     6481 gtttcattga tgacatataa aggaatactg agagtcaaat aagaagactc ataacgttca
     6541 gcagcttttt gaactaatcg tgtggagcca gctaaggaat tatattttcc gatttcgcca
     6601 atgaattgag taaaaattaa ttcttcacga gcacccattt taaactgatc aatcactttg
     6661 taaacggtat ctccccaaga tagtgcaacc acattggctt tcttaattaa ttcttgaatg
     6721 taatcggcag caaattttga aaataataaa tcgtgttgtg ataagtcttc actacttttt
     6781 aatatataga tattcgtttt aaagtaattt ttgaagattt cttctagctc ataattccta
     6841 gcatacggag aattaattga aatactaact aattgttttt cacgtgcatc ttcaatatat
     6901 tttaaagctt tatatctggt gatcccatat tttttagaaa tttcactaat attgatatcc
     6961 tcgagataat agtcttcaca aatttttaca aggagtgatt catagtcaga gaattccaga
     7021 tgcatagtat agctcctaac tttttcaaat agatattttt tctcattata ccaaaaaaat
     7081 tgaatttagt ctttttttaa atttttaaaa ataagcatca tttttttaaa atttaaaatg
     7141 aaagggtttt tttattttaa aaaaagtgtt aaagtggttt tgtaattaaa aaatgtaaaa
     7201 aggagtgaag gtatgcttga tgaagtttta gaaatccata aaaaaaatgt aggtgtttta
     7261 cggatggaaa caacagaagc cgtgcaagat gatcaagatt tagcaaaaat gtacacccca
     7321 ggtgtagcag aactaagttt aatgattgag aaaaatcatg ctttggcaag agaattaaca
     7381 attagtggta aattagtggc cgttattaca gacgggtcgg cggtattagg tcttggaaat
     7441 gtaggaaacc aagctggatt accaatcgtc gaagggaaag cgctgcttta taaaaattta
     7501 gcaggtgtca atgcgattcc attagccatc gaacaaaaat cagtagacga gattgtacaa
     7561 acaattgtca atttgcaaaa tagttttgca gggatccatt tagaagatat tgcagcaccg
     7621 aagtgttttg agattgaaga aaaattacaa gaaaaacttt ctattcctgt gtatcatgac
     7681 gatcaagaag gaacagcgat agttgtttta gctggattga tcaatgctgc taagatccaa
     7741 aggaaaacat taactgaact tcgagttttg atcaacggca tgggagcatc tggggttgca
     7801 acagctagac tattaatcgc tgctggtatc aaaaatctaa cattggtgga taaacaaggg
     7861 agattaaaga agcaagatag tacattaaat aaatatcaaa gagagttgtt ggatcatata
     7921 catgatacaa ataataatgc aagcgttttg gaagatgcaa tcgttggaca agatgttttt
     7981 atcggtcttt caataggaaa tgttttaacg cctgaaatga ttggaaaaat gaatgataat
     8041 ccgattattt ttgctttagc caatccagta ccagagatca cacccgattt agcaaaagaa
     8101 tctggtgcaa agctaatcgc tacaggttct tcgaaatatc ctaaccaagt aaacaatatt
     8161 ttagtatttc ccggattgtt caagggacta ttactatcga aagcgaataa agtcgatctt
     8221 tcattacaac ttgaagtagc caaacaatta gctgctaagg tcgcagagcc tacggtagaa
     8281 aaattcatcc ctaatgtttt tgatgaagga gtcgcaaacg tcgtttcaca ggcagttttg
     8341 gattatttac aaaaactaaa agaaaacgag gataatatac atgctgaaaa cattaaatag
     8401 aattaaaatt ggttttattc ctttatctct ttatatcatt ttggtactcg cgtatcttgc
     8461 aatgacgata agcaatatcg ttccagaaaa tgatatgtta gtttctttgg gtattatgac
     8521 tgtttttgct tatttattag aagaaattgg taaaactatt cctattttaa aagatttagg
     8581 tggtaaagta ttagttgtta catttttgcc tgcatttcta gtctataaac attggctacc
     8641 tcaaagtact gtaactgtgg tttctgattt tatgaataat aacaatttct tgatgttctt
     8701 tatcacacta ctagttgtcg gcagtattgc ttcaatgaat cggcaaacat tggttaaagc
     8761 aacttcacgt attatcatta ttttaatttt atgcgatgtg attggttctt tgataggaac
     8821 aggtgtggga atactccttg ggctatctgc ttggaatgcg tatttcttta tcgttgcgcc
     8881 gattatggct ggtggtgttg gggaaggagc gatgccatta tcgattggtt atgcagcaat
     8941 attatctggt atgggacaaa atgatatctt tgggaagatt ctaccgtgtg tgatgttggg
     9001 aagtttagtc gcagttctac tgtctggtat gctcaaaaaa attggagaga aatacccaca
     9061 attaaatggt cagggtaatt tgatcgatgg ggatgatgaa catttagaga acttgaaggg
     9121 ttcagctaaa ccagtcaata ttgaaaaaac attgttagca ggcgtggtag caataacgat
     9181 ttattttgtt gctgtctatg ttgagcactt attaaaatca ctccttggct tttctattcc
     9241 ttcacctgtg atcttattag tctctcttat gggggctaaa atgcttggtt ttatccctaa
     9301 aaatatcgaa gaaggtggaa attcgttgta tggattcact gttcgtggga tcacacctcc
     9361 cttacttttt ggcgtagggg ttgcaaaaac agattggtta gagctgatcg aagtatttaa
     9421 aaatcctgca acgatagtaa caatcattgt gacggtagta gctattgtcg ctacagcttt
     9481 tatcgccgca aaatttttaa aaatgtaccc agttgatact gccatcgcag tttcatgttg
     9541 tagcggacaa ggcggtacag gtgctttagc tatcttagct gcgggtgatc gcatggaatt
     9601 aatgccattt gcacaagttg ctgttcgact agggggtgct atgaccgtaa cgttcgctat
     9661 ctttttaatg ggtctgctat cataagaaca taaagaaagg acgtgaccat gtgtttgatc
     9721 aaacagatat ccaacgatta tttttaaaaa ataactatat caaagaccaa tgggaaaatc
     9781 ttttgcttga ttcaggaata caaaagaagc aaatcgaaga ttttcaacat ttagatcaaa
     9841 cattgggtat ataccataaa gaaaagttag taggaacagt atcttatcaa aacaacgtga
     9901 ttaaatatat cgccgtgtct gaaaaatata aagatggtgg acatacattt aatgtactag
     9961 tctctgcaat aattcaggca atgagtcaac aagagatttt tcattatatt gttttcacga
    10021 aaccgcgcta tcaaaaaagt tttaactttt taggttttga aacaatcgta tctaccccaa
    10081 aaatagctgt attagaaatg ggagatagtt ctataaaaca gtacctagag aggattccta
    10141 aagtgaatgg tgaaaaaaaa gcagcaattg tcatgaatgc gaacccattt acagaagggc
    10201 attattattt agtaaaacaa gctgccaata acaacgattg ggtatatgta tttgtactcg
    10261 aaaaagaaca aaatcttttt tcgacgaagg accgactcaa attagttcaa caaggattac
    10321 aaaaactgaa aaatgtcatt gttctatcag gtggcgagta tattattagc cctgcaacgt
    10381 ttcccaccta ttttttacga gaagatgatg aaatagcaaa ggagcaaatg gtcgtagatg
    10441 ctactttatt caaagaaaga attgccactt atttagatat ctctgctaga tatgtgggag
    10501 aagaaccact ttccccaatg actcgttcat ataacgacat actggaaacg attttacccc
    10561 ctgaaataaa agttcatgtc cttgcaagga agaaaacgga acaacaccaa gttatttctg
    10621 cttctcaagt tcgtaaagcg tatcttgctg ggcaattgga aaaaataaaa tatatggttc
    10681 cagaaacaac ctatcaatac ttaaaaaata aaagggagcg ttaagtaatg gaaataaata
    10741 aatcagcgat tgctggaaca ttagagtcaa gtgatgtcca aatcatgatt tcaccgaata
    10801 cagaaggaat agaaatcgat ttagacagtc aagtgattga gcaatttgga aaacaaatca
    10861 aaaaagttat ctatgacaca ctagcagttt atcagattaa aaacgtaaaa ctcaaggtag
    10921 tagataaagg ggcattagat tgcgttattg ttgcacgatt aaaaacagcg atccatcgtt
    10981 ccttaggtat ccaacaccaa gaaacaccgt gggaggttta attagatgat gaaagaacgt
    11041 ttgagaagaa caatgatgtt tgttccagga aataatccag cgatgatcaa agatgcaggt
    11101 atttatggtg ctgactcgat tatgttcgac ctggaagatg cagtgtctat ggcagaaaaa
    11161 gacagcgcaa gaatgcttgt ggctgaggca ttgaaaacag tggattatgg tgacacagaa
    11221 ttaattgttc gaataaatga cctgcactca gaatttggac gtgcagacat ttttgcaatg
    11281 gttaaagccg gcgcgcatgt attacgttta cctaaaacag aaacctctga agatataaca
    11341 gaagtagaaa aagtgattat agaagcagaa aactatcata atgttccgat agggcgaaca
    11401 ttgatgatgg cagccattga aggtgcttct ggtgtattga acgcacctgc gatcgccaaa
    11461 gcatctgatc gattgattgg aatcgccatt ggggcagaag actatgtgac aaatatgaaa
    11521 accaaacgat atccagatgg aaacgaattg ttttttgctc gcagtatgat cgttcatgct
    11581 gcacgtgcag caggcatttc cgcatttgac actgtttact ctgatgtgga tgatatggaa
    11641 ggatttttaa acgaagtacg tttaatccat caattaggtt ttgatggaaa atcttgtatc
    11701 aatccacggc aaattccagt agtcaatact gtttatcagc cgtcaatcaa agaaatcaaa
    11761 cgatcactag acatcatctc ggcaatcgaa gaagcaaaag aaaaaggttc tggcgtaatt
    11821 tctttaaatg gaaaaatgat cgataaacca attgtagaac gtgcacaacg gacattgatg
    11881 ttagcaaaag catcacgatt gattgatgag gagggaaata tccatgcata ataaacaaag
    11941 acgtagcatt ccatcaagtg ttttaacaaa gtataaaaaa gaattccaaa gtgtttcatt
    12001 atcgccacaa acgattcaac gttgtgcacc aaaagtaacg actgtaacta atcagacaaa
    12061 taaattgatg cactcgatca aagaagctat agaaaaatcg ggcttaaaag atggaatgac
    12121 gatttcattt catcatcatt tgagagaagg tgattttgtt tttaaccaag tgatggccgt
    12181 gatccgtgaa atgggcatca aagaattgac cttagcacca agttctttaa cgaacactat
    12241 gaatgagatg gttattgaat gcatcaagga cggtacagta actcatatca caagcagtgg
    12301 gatgcgcggc agcttaggtg aatttatttc tcatggagga atggacaatc ctgtgatttt
    12361 acgttctcat ggtaatcgtc cacgtgcaat tgaaagtggt gaattgaaaa tcgatgttgc
    12421 atttctagga gtaccaacat ctgatgatta tggaaatgct aatgcgactg ttggaaaatc
    12481 cgtttttggt tcattagggt atgcaaaagt agatgcacaa tatgcagata aagtcattct
    12541 tctaactgat catttagttc cttaccctaa tacgcccatt agtattccgc aaacacaagt
    12601 cgactatgtc gtggaagtag aagcaattgg tgatccgaat aaaatcggtg ctggtgctac
    12661 acggtttact aaaaacccaa aagaattaaa aattgctgaa atggtcaaag atgtcattgt
    12721 tcactcacct tatttcaaag atggcttttc ttttcaaaca ggaacgggtg gtgcagcttt
    12781 agcagtaacg cgttacttag ctagtgaaat ggaagaaaaa cagatcaaag cttcttttgc
    12841 attaggtggc atcactaaac caacggttga tttattggaa aaaggtcttg ttgaaaaagt
    12901 gttagatgtc caagactttg acaaaggagc agctgaagga atggcgcggc ttgagaacca
    12961 acaggagatc gatgcttcct tttatgctga tccaagtaat aaaggagcgg ttgttaacca
    13021 attagatgtc gctattttat ctgcattaga aattgacaca aacttcaatg taaatgttat
    13081 gactggttca gatggtgtgc taagaggtgc gattggtgga catcaagatg cagcaacggc
    13141 taaacttact atcatctcag cgccgttagt acgtggccga atacctacag tagtaaatga
    13201 tgttactaca ttgatcacac cagggaaaag tatcgatgta ttagtcacag aagtaggggt
    13261 tgctatcaat ccccaacgta aagatctaat agctattttt gaaagaattc cacaaattcc
    13321 tgtttttaca atagaagaat tacagcaaaa agcagaaaaa atagtaggca tcccggaacc
    13381 tttgcaattc acagatcgga tagttgccta tgtagaatac cgagatggtt caattttaga
    13441 tgtcgttcgt caggtgaaag aatttgaaga agagagatca tgatgtttga tactggagaa
    13501 attcaagaaa tcgaggatat tttgatccat aaagaacaac gtgctaactt gcaaagcaac
    13561 ctgctttcca agtattcaaa tagttcattg atggtcttta cattgaatat tcctgggccg
    13621 atcaaaaata atccaatgat tgagcaggta tttcgtcagg gacagcagtc aattcagcaa
    13681 atgtttttaa aaaaaaaggt ggaaatgacg tacacaaaag aaatcaattt gccaagtgga
    13741 ttggactggt acgtaatagt aacagaagca cctttgaatc tcaaaaaatg gttggtcacg
    13801 ttagaagagc aagacgcatt aggtagactt tttgatttag atgtcttgtt caaaaaagga
    13861 gagcagataa caattgtcag tcgccaagct cttaatcatg gggaacgtcc ttgttttatt
    13921 tgtgggaaat cagcaaaggt atgtgcacgt aatcgtacac atactgtgaa agaaatgcaa
    13981 aagaaaatcg aacaaatagt agccgattcc ttaaaaggag cattgaaaca tgcatgataa
    14041 tattcaaaaa atagcacaag aagcacaatt agcgatgctt tatgaagtta catgtatcaa
    14101 taaaccagga ttggttgatc cagtagaccc cgggtctcat caagatatgg acatttttac
    14161 ttttctacaa agtagtgtag ttttgactcc ctattttgaa gaatttatcc aaacaggatt
    14221 ggaccgacaa catcaaccaa ttgaagaaac ctttatggcg attcgacaga ttggtttaga
    14281 ggctgaaact gcgatgttct cagtgacaaa tggtataaat acacataaag gtgctatctt
    14341 ttcattgggg atttttcttg ctatttgtgg ccggttaact atttggaaag ttccttgtaa
    14401 aaaaagtttc tttcaaaaag aaattcaaaa aatgacgaaa aatctactta acgattttgg
    14461 gaagattgat caaacaaaac caaaagcatg gacttgggga gaatatctat ttgttaacca
    14521 tggattgaca gggatacgag gggaagccaa aagaggttat ccttgtgttt ttgaccgagg
    14581 attgccttat tatcaagcat atcaagggtc gcaacaagat aagatgatag acactttact
    14641 ttttctatca atggaagtag aagataccaa tttgatcaaa cgaagtaata atagacatgt
    14701 ttttgaagag tatcaattac ttattcgtcc ttattttgaa ttagggggtg taaaaaccat
    14761 acaaggtaaa aaatatttgg attacctaaa tcagcaattt aaaaagaaaa attggagtat
    14821 tggtggaagt gcagacttat tgatccttac aatcttttta gataaaatca tggacaaagg
    14881 atggttatgt tgatactata atacattatt ttgtattagt aagctaataa ctaaaaaatg
    14941 gttcaataca aatattttat aaaaaatggt agtgcctatc ctttcagatt gtagacaaag
    15001 aaaaatttct ttgtctacaa tttttttgtt aatgacttca gtcagtttcg tgcgcaaagc
    15061 gtgcgaaaca agaaaaccat gcgtttctac acgtggaaga actagcatat cgaaaggagt
    15121 tttttcatgt caaatgacga taaaagttta gcccatactc gttggaattt taagtatcat
    15181 attgtattta caccaaaata tcgacgaaaa gtaatctata ggcaattacg aaaagatatt
    15241 ggaaaaatac ttaggaagtt gtgcaaaata aaaaacgtgg aaattataga agcacatgca
    15301 acgccagatc atattcacat gcttgttcaa gtaccatcaa aaataagtat atccagtttt
    15361 atagttatct gaaagatcgt agtgctgtgc taatccacga gcagcacgta aatcacaaat
    15421 atagatacgg aaataaatca ttttggtcgg aggaatatta tgtgagtaca gtcgaattga
    15481 atcaaaagac aattgaaaaa tacatccgtg aacaagaagc tgatgatcgc atttgagata
    15541 gtataagtaa aagagagtag atgttgcaat aacaaaaggg taaaaatgga ctacctccag
    15601 taaaataccg aaaaatagtt taacttttgg agataagtcc atttaagggt ctatagaatg
    15661 acaatttttt taggggattt tactattatt acttcaagac ccgcttggat tcgcaaagtg
    15721 agtgaactct taaaactacc tgatagtagg ttgttttaag agttatctga aagtaaaaat
    15781 tgcatttata tttttgataa atacgtaaat ttatatatac gtatttatca aagttttagg
    15841 ccaaaggaat attgtgtaaa tacactagga ttgaatgaat agaccagaag aaattgcgtc
    15901 caagaacaaa agaagcatga tagtgctcta tataaattgg gcattcaaga atctgaaatt
    15961 taaaggtaga attttagtta gacttttaga ttttaaacac aaaaataacc atacaagcaa
    16021 caagaagcaa agtgctatat tataaattgt ccgtccgttt acttacataa gatcattggg
    16081 gctattttta aaagagaata atagataata aatgaaaaaa aagtcaagtc cacaatcctg
    16141 tgtaaaatac tatacaatgc tctcactatt ttttacttgg caaatttaat atattttctt
    16201 gatgaagaaa gccactcatc gtgttgatca ataaggactg ctccaattaa acgattggct
    16261 gaaacgcagt ttggaaagat acgaataatt ttttctcttc tgcgcacttc ttgattcagt
    16321 cgttcaatta gattggtact ctttagtcga ttgtgggaat ttccttgtac ggtatattga
    16381 aaggcgtctt cgaatccatc atccaatgat gcgcaagctt ttgaatattt tggttgatcg
    16441 atataatcat gaatcaatcg atttttagcc tcacgagcta agttaatatc tgtgaactta
    16501 aaaattcctt taacagcttc tctgaaagat tttgaatttt ttttaggaat ggtggtaaag
    16561 atatttctta ggaagtgaac ttggcatctt tgccaacttg cgttggtgaa ggattttcta
    16621 atggcagaga ctaatccttt gtgcgcatca gaaataacga gttctgtacc ttgtaaaccg
    16681 cgttctttta ggtattcaaa aaatgttatc caggtctctt cgctttcgcc actttgaatc
    16741 atgaagccga taatttcacg gtcgccatct ttggttattc caatcgctat atgacagctt
    16801 tttgagagta ctcgattttc ttctcgtact tttatataga gtacatcggt cattaagtaa
    16861 ggataatttt tttctgataa taaacgattc tgccactcgt taaccattgg ttctagctgt
    16921 tctgttaagc tagaaacgaa ggacttagag acggatttac cacaaagttc ttccacaatt
    16981 tttgatactt tacgagttga aacgcctgat acatacattt ccaacattga agcaatgagg
    17041 gctttttcgt ttcgttgata acgttcaaac actgtgggtg aaaaatggcc atcacgtgtt
    17101 ctgggtactt ttaattctag cgtgccta
//
LOCUS       DAGCYL010000043        17059 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.044, whole genome shotgun sequence.
ACCESSION   DAGCYL010000043 DAGCYL010000000
VERSION     DAGCYL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.044"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            32..1000
                     /locus_tag="MB216_001108"
     CDS             32..1000
                     /locus_tag="MB216_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4525958.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            1113..1391
                     /locus_tag="MB216_001109"
     CDS             1113..1391
                     /locus_tag="MB216_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBT4525959.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            1509..1745
                     /gene="secG"
                     /locus_tag="MB216_001110"
     CDS             1509..1745
                     /gene="secG"
                     /locus_tag="MB216_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBT4525960.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            1848..2603
                     /locus_tag="MB216_001111"
     CDS             1848..2603
                     /locus_tag="MB216_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4525961.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2606..4963
                     /gene="rnr"
                     /locus_tag="MB216_001112"
     CDS             2606..4963
                     /gene="rnr"
                     /locus_tag="MB216_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBT4525962.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            4985..5449
                     /gene="smpB"
                     /locus_tag="MB216_001113"
     CDS             4985..5449
                     /gene="smpB"
                     /locus_tag="MB216_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBT4525963.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5751..6470
                     /gene="atpB"
                     /locus_tag="MB216_001114"
     CDS             5751..6470
                     /gene="atpB"
                     /locus_tag="MB216_001114"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBT4525964.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6523..6738
                     /gene="atpE"
                     /locus_tag="MB216_001115"
     CDS             6523..6738
                     /gene="atpE"
                     /locus_tag="MB216_001115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBT4525965.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6829..7353
                     /gene="atpF"
                     /locus_tag="MB216_001116"
     CDS             6829..7353
                     /gene="atpF"
                     /locus_tag="MB216_001116"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBT4525966.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            7340..7882
                     /locus_tag="MB216_001117"
     CDS             7340..7882
                     /locus_tag="MB216_001117"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBT4525967.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7905..9461
                     /locus_tag="MB216_001118"
     CDS             7905..9461
                     /locus_tag="MB216_001118"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBT4525968.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            9475..10377
                     /locus_tag="MB216_001119"
     CDS             9475..10377
                     /locus_tag="MB216_001119"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBT4525969.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10400..11806
                     /gene="atpD"
                     /locus_tag="MB216_001120"
     CDS             10400..11806
                     /gene="atpD"
                     /locus_tag="MB216_001120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBT4525970.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     RGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11820..12242
                     /locus_tag="MB216_001121"
     CDS             11820..12242
                     /locus_tag="MB216_001121"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBT4525971.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            12711..12941
                     /locus_tag="MB216_001122"
     CDS             12711..12941
                     /locus_tag="MB216_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBT4525972.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            13038..14339
                     /gene="murA"
                     /locus_tag="MB216_001123"
     CDS             13038..14339
                     /gene="murA"
                     /locus_tag="MB216_001123"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBT4525973.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            14352..14528
                     /locus_tag="MB216_001124"
     CDS             14352..14528
                     /locus_tag="MB216_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBT4525974.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            14663..14959
                     /gene="yidD"
                     /locus_tag="MB216_001125"
     CDS             14663..14959
                     /gene="yidD"
                     /locus_tag="MB216_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBT4525975.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            15023..15406
                     /locus_tag="MB216_001126"
     CDS             15023..15406
                     /locus_tag="MB216_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4525976.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            15575..16459
                     /locus_tag="MB216_001127"
     CDS             15575..16459
                     /locus_tag="MB216_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBT4525977.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            16573..16809
                     /locus_tag="MB216_001128"
     CDS             16573..16809
                     /locus_tag="MB216_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBT4525978.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            16897..16977
                     /locus_tag="MB216_001129"
     tRNA            16897..16977
                     /locus_tag="MB216_001129"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16931..16933,aa:Tyr,seq:gta)
     gene            16984..17055
                     /locus_tag="MB216_001130"
     tRNA            16984..17055
                     /locus_tag="MB216_001130"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17016..17018,aa:Gln,seq:ttg)
BASE COUNT         5675 a         2923 c         3780 g         4681 t
ORIGIN      
        1 ttatagtttg gcagaaagcg gcgggtgtac gatggttatt ttttatttca ttcttattct
       61 gatatttata ggaatcagtt accattttct ttcttatttt tatgcaaaga aattagtaag
      121 agcgggggta cagagaggct atgactggta tgaagaaact gggcaccgct tgatggatcc
      181 tgctgcagtc acgtcagtag aaaaaaagcg acaaaaatta gaagaagagt gtgaaagctt
      241 ctgggaacaa ggaataccga aaacgatcaa aagttatgac gggttgaaac tagctggaca
      301 gatgttcctc caaccaactc agcagaataa atgggtcatt tgtgtacatg attatcgaag
      361 tacagggaaa agagatatgt ctcatatagg taaaagatat gcagagaaag gattcaacgt
      421 tcttataccc gatttacgtg cgcatggaga aagtgaagga gaaatcatcg gaatgggatg
      481 gctagaccgg ctagatttaa ttgcatggat ccaactcatc ttagatgaac aaccagatgc
      541 atccattatt ttgcacggca gttcgatggg cgcttctact attatgatgg caagtggtga
      601 aaagcttcca tctgcagtca aaggatttat cttagacagc ggatatgttt ctgtttatgc
      661 ggaatttcgg tatatgttaa gtaaaatcac ggtctttcct aaaaaaatga tcatgagata
      721 tgcgaatcac tatgctcaga aatacgcagg ttattcttta aagcaagcat cagctacaag
      781 acagcttggt agtaaccatt tgcctttgtt agtcatccat ggtgaaagag atcattttgt
      841 tcctacagaa gcagcataca cgattcaaaa cgctacggca ggggataaag cattactgct
      901 tgttccagaa gcagaacatt tggaagcatc gatgaaagat ccgaaaacct attggacagt
      961 gattttttca tttattgaac aaagaataac gatggattga ttttctcttc ctttttgtaa
     1021 gcaacacgag gataaataat tgtttataga ccatgagact gttaaaataa ggtaggaata
     1081 aaaaaatatc agttatttga ggagtgcggc atatggaaat caaagaggaa aaaaatcgtc
     1141 ttgctttatt caatgatgag caacaagaaa tcggagaaat gacatggtcg gatgcggggc
     1201 cagacatcat gatcattgac catacatttg tcgatccagc atatcgtggt caaaaattag
     1261 ctgaaaagct agtgtatact ggagtagaac tagcaagacg agaggggaaa aagattatcc
     1321 cttcatgccc ttacgcaaaa aaagagtttg agaagaaacc agagtatcat gacgtcttac
     1381 gccaatcata ataataacag ctgaaagatt tgcactttca gctgtttttc catttatata
     1441 gagttgtcac ggagttttat ctatgataaa gttaaagaga cgatatcttt tgggaaggag
     1501 gctgctatat gtataattta attttgggaa tcgtgatcgt tttatcgatt gttatggtca
     1561 ttgcgattat gatgcagcca agcaagcaaa atagtgcagc gagtgcattt accggcggtg
     1621 cggatcaact attcggcaaa caaaaagctc gcggctttga agctgtcatg cagcgatcaa
     1681 ctgcagtcat gggagctgtc tggatgatct tattatttgt cctagcattt ttatcttcga
     1741 agtagtcgaa taagcctctc gcacaacggc gggcggcttt ttttattgca aagagaacgc
     1801 ttaagaagaa gttatgaaag aaactatggt aaaatgaggc gaaagaggtg aaacgagtga
     1861 aacaattacc aaaacccctt tatgcaaaac atggaaaacg agcagttttg ctgcttcatg
     1921 cgtactcagg aagtccaaat gatgtccgga tgctcgcacg gtttttagaa aaagcagatt
     1981 atacagtcta tgcgccgcta tttaaaggac atggaacgat ggagccatat gatatcttgc
     2041 aagaaaaagc agaaagctgg tgggcagata ctaaaaaagc tatccatttt ttacagtctg
     2101 aacagttttc tgatattgcc gtattgggat tatcgatggg tggcatcttt gctgtacggg
     2161 cactagaaga agaatcagta attggcggcg gttttttctg ttcgcccctg tctcctgtag
     2221 aaacaaacgt gcctgaaaat tttgaaaaat atgtacgtca agtgctgaag atagcaggca
     2281 aatcagaaaa agaaatcaat gaaaaagctg tgtcttaccg ctcgttagct gaacatcaat
     2341 taatggatat acaagaccaa gcagcaatcg cagaaagtag actatcagat atccaacagc
     2401 caatattttt agctcaagct gggaaagatg aaatgatcga cccaaatggt gtatttgaaa
     2461 cggcacgcaa attgagccgc caacgagtga cacttcaatg gtatcctgaa agcggtcatg
     2521 tgattacagt tggcacagca agacgcgaat tagaaaaaga cgtgttggaa tttttagaaa
     2581 aattaccttg gaatgaggaa taaatatgac aaaagaaaca ataaaagaga aaattctctt
     2641 tttcatggaa agcagtcgca aacgtagttt ttctatggaa gaaatcgctg aaggtctagg
     2701 ctttcaaaaa agcgatgatt ttaaattact agtacaaacg attgctcaaa tggaaagaga
     2761 acagagtatc gttttcaata aaaaaggaaa agtcaaactt cctttaaaac cgatcttggt
     2821 cgaagggaca tttcgggcaa atgaacgagg atttggattt gtaacgattg atcccgaaga
     2881 agatgatatc tatatcccga aagaagcgac tggctttgca atggatgggg ataccgtagc
     2941 aattgatatc atgaagcgag caaatacaga tatggatcgc ggagcagagg gaaaagtagt
     3001 ggagattcga gaccgagcga ctactcagtt agccggagaa tttgttgctt attcagacga
     3061 agagatacag gaaacggatc tttatggtgt ggtcatcccg aaagatagaa agcttaacca
     3121 attcaaagtg tatgcagcgg cagagggagt tcgtccagtt gacggaagta ttgtattgat
     3181 cgaactgacc cattaccctg aaaaagggta tgcaactagt ttagaaggga taatcaagca
     3241 agtgattggt cataagaatg accctggcat ggatatcctc tcgattgtcg tggctaacgg
     3301 tattcctaca aaatttcctg atgatgtgtt atctgaggca gatgaagttc cagaccagat
     3361 cagcgaaaag gatattgttg gacgcagaga tttaagagat cagctcatcg ttacgattga
     3421 tggagaagat gcaaaagatc tagatgatgc agtgacggtc aaaaaattag aaaatggtaa
     3481 ttattttctt ggagtacaca ttgcagatgt atcttactat gtgacgcaag gcagtcaact
     3541 ggacagagaa gcatacgaaa gaggaacaag tgtctatttg accgatcgag tgattccgat
     3601 gatcccacaa cgtttatcca atggtatctg ttcattgaat ccccatgttc cacgactcac
     3661 catgagttgt gaaatggaga tcgatccaaa tggacaagtt gtctctcatg agatttttcc
     3721 aagtgtgatc caaacaacag aacggatgac ctacacagct gtcaatcaaa tcttagaaga
     3781 tcaagatgaa gaaacgatgc aacgttatgc tcagctcgtg cctatgttcc aagaaatggg
     3841 tgaattgcat cgaatccttg aaatgatgcg aatcaaacgt ggagcgattt cttttgagga
     3901 tcgtgaagcg aagatccttg ttgatgctga aggacaccca aatgatatcc agctaagaag
     3961 cagagggata ggtgaacgct tgatcgaatc ctttatgctt gctgccaacg aaacggtagc
     4021 tgaacattat aataaattaa atcttccgtt tatttatcga atccatgaac agccaaaaga
     4081 agaaaaaatg cagcgcttct ttgattttgc ttcagctttg ggcattctgg tccgaggaac
     4141 gaaaggaacg atcacaccaa aagatttaca gcatgtgatc gaaggagtag aagacaaacc
     4201 agaagctgca gttatcaata ctatgttgct aagaagcatg caacaagcac gctattcaga
     4261 agacaatttt gggcattatg gcttagcggc agattactac acacacttta catctcctat
     4321 tcgacgctat cccgatctaa tcgtccatcg tctgatccgt tcttacagtg aagataaaag
     4381 tgaaagcaat caaagttcgt gggctgcgga acttcctgaa attgcagatc atagttctaa
     4441 gatggaacgt cgtgcagtag aaacagaacg tgaagtagat tccatgaaga aagcagaatt
     4501 catgatggac aaagtgggag aagaattcga tgggatcatc agctctgttg tgaaattcgg
     4561 cctgtttatc gaactgccga atacagtaga aggattgatc catatcaatg aattgaaaca
     4621 ggactacttc cattttgttg aaaatcattt agctcttgta ggtgaaagaa ctggtctgac
     4681 ttttaaaatc ggacaaaaag tgcgcgtaaa agtagtcaaa gctgatccag atgaacgagc
     4741 agtggatttt gaactcgttt ccgcagaaga ggtcactgcg ttggaacgtc caaaagcacg
     4801 caatggaaaa tctggaaaac gagaaggaaa acaagcaggc aaacgctcag aaaaaaatgg
     4861 caagaagaac aaacgatata gtgatagtaa gcaaacgttc ggtaagaaaa aagggaaaaa
     4921 gccattttac aaaggtgtca aaaagaagaa aaagaaaaaa taaaatgagt tggaggaaac
     4981 agctatgcca aaaggcgagg gaaaactaat tgcacaaaac aagaaagctc gccatgatta
     5041 ttcgatcatc gacacgatgg aagcagggat ggtcttgcaa ggaacggaga tcaagtcgat
     5101 acgaaacagc cggatcaatc tgaaagatgg atttattcgc gtccgcaacg gggaagcttt
     5161 cttgcataat gttcatatca gtccttatga acaaggaaat atttttaatc atgatccgtt
     5221 gcgcacgaga aagttattat tacacaagaa acaaatcgtt cgtcttgaaa acgaattgaa
     5281 aaacactgga atcactgttg ttcctttaaa agtctatatt cgtaacggct atgccaaggt
     5341 attgattggt ctggcaaaag ggaaaaaatc gtatgataaa cgggaagatt tgaaacgaaa
     5401 agatatcgat cgacaaattg atcgaacatt aaaaaatttc tctagataat ttcattatcc
     5461 tttttcgcat attcggtgct tttgctttta cttaatgata aaatttgtta gcatttaatc
     5521 tgataaaaca tgattatcac aggttttttc ttagatcagg agcaccgtgc atggggaaaa
     5581 attcgcttcg tttttgactg aaataaaaaa gtgtctaatt ttcatgaaaa tgttttgaat
     5641 tttctgtttc atggtggtat gataccgtgt ggttagaaac cgattggaaa aaaccagata
     5701 cgcacatgaa ttgattttat gtgaatttcg gggaaaagag gtgaaatgga ttggatgaac
     5761 gctcgctaac gtttcacatt ggaccaattt ggtttgatgg tacagtttgt ataatggtgt
     5821 tactgacctg tttgattgtt ttcttcttag cttacttttt tacaagaaat cttaagttga
     5881 aaccaacagg caaacaaaat gcgatagaat gggtcattga ttttagtcga ggaatcgtaa
     5941 cagataatct gcctagaaaa gaattgagca atttccattt gttggcattt acattgtttt
     6001 tatttgtttt tgttgcaaac aacataggtt tagtaacaaa aatcgtctta cctaacgaaa
     6061 caacactttg gaaaagcccg acagcagacc cgtttgttac tttaactttg gcatttataa
     6121 tgatcttact tactcactta ttcggggtga aaaagttagg cttcaaagga tatttcgtaa
     6181 attcgttctt gaaaccctat agttttatgt ttccgatgaa actgattgaa gaatttacaa
     6241 acttattaac acttgcttta cgtctttatg ggaatattta tgcgggggaa gttttgctaa
     6301 ccttgattgc aaaaatgatg acaagcttag gctggttctc attaccatta gcaattccat
     6361 tagagatggt ctggatcgcg ttctccttgt ttattgggag catccaagca tttgtctttg
     6421 tcactttgtc aatggtttat atgagccata agattgaagt agaagaataa tactcggtta
     6481 aaaaacaaac tactatttat ctattttagg aggaaaacaa ttatgaatta tatcgcagca
     6541 gcaatcgcaa tcatgggagc agcaatcgga gccggttatg gtaacggtca agttatctct
     6601 aaaacaatcg aatcaatggc tcgccaacct gaaatgtctg gtcaattccg tacaacaatg
     6661 tttatcgggg tagctctagt tgaagctgtt cctatcttag gggtagttat cgccttgatt
     6721 ctagtttttg ccgtttaatt gaatcagtat taaggcagaa acaacatcgt ttgttactgc
     6781 ttttttacat ggaaactaga aatactccaa agaaaggaag gctcgcctat gctgaatcaa
     6841 ttggcaattg cagaagttgg caatgcgatg ttaggtaata tcatcgttgt cagcggctca
     6901 tttttgatac tactggctct cttgaagcac tttgcttggg gaccaatcag cgatattttg
     6961 aaaaaacgtg aagacaagat cgccaatgat ttagattctg cagaacaatc tcgcatcaac
     7021 tcagcgaaaa tggaacaaga acgcgaacaa caattgttag cctctcgttc tgatgcagct
     7081 gatatcatca aaaatgcgaa agaaagtgga gaattaagcc gccaaaatat tttgaaggat
     7141 gctcaagaag aagcagctcg tctaaaaagc aaagcccaag ctgatatcac tgtagaacgt
     7201 gattcagcgc tgaactctgt aaaagacgac gttgcagaac tctctcttca aatcgcggaa
     7261 aaaatcttga acaaagaatt atctccagaa atgcatgaat cattaatcaa tcaatacatt
     7321 gaaggtctag gttcttcaaa tgaaactaga taaatataca gtaggaagac gttacgggaa
     7381 agctttattt gaactggcga tcgattcaaa tcaagcagaa gaaatctatc aagatctcgt
     7441 aagtttacgt caaatctatg aacaagtacc tggattaggg aatatgttaa gtgatgttcg
     7501 tctagaacct catgaaaaac gagaaatcat ggacaagctt gtcagcggtt atgatggaat
     7561 cgttaaaaac tttttagaag ttgtttatga gtataaccga atggatgacc ttttgttcat
     7621 gattgaagaa tatgaacgtc gatatgatga acatcaaggg ttactattgg gaagtgtcac
     7681 aacagcagtc ccactttcag acgaacaacg aatacaatta gaaaagaatg ttgcaaaaac
     7741 aatggactat caatcagttg aattaaaaca aatcgttgat tcttctatta ttggtggcgc
     7801 gattgttgaa gccaaccatc gagtaatcga cggaagcatt cgcacgcaat tagaaaaaat
     7861 gcgtaatcaa ttaaacagat agtacaaaga ggtaggtgaa tcgaatggcc atcaaagcag
     7921 aagaaattag tgccttgatt aaagaacaaa ttgaaaatta tcaaaacgtg ctttcagtcg
     7981 aagaaatcgg taccgtaact tatgtaggtg acgggatcgc tcgtgcacat ggtttagaaa
     8041 acgcgatgag tggtgagttg cttgaatttt caaacggctc atacggaatg gcgcaaaact
     8101 tagagtcaaa tgatgtaggt atcattatcc ttggcgattt cgagtccatt cgtgaaggag
     8161 ataaagtaaa acgaacaggt aagatcatgg aagttccagt tggagaggcc ttgatcggtc
     8221 gggtagtcaa tccgctaggt caaccaatcg acggactagg tgaaatcgtt actgacaaag
     8281 ctcgtccagt agaagcgatg gcaccaggtg ttatgcaacg taaatctgtt aacgaaccaa
     8341 tgcaaacagg gctaaaagcg atcgacgccc ttgtgccaat cggacgcgga caacgtgaat
     8401 tagtcatcgg tgaccgtaaa acagggaaaa cttctattgc gattgatacg atcatcaacc
     8461 aaaaaggtca agatatgatc tgtatctatg tagcaatcgg acaaaaagat tctacagttc
     8521 gtacacaagt tgaaacattg aaaaaatatg gcgcaatgga ttacacaatc gttgtgaatg
     8581 ccggtgcgtc tcaaccagca ccattacttt atatcgcacc atatgctggt actgcaatgg
     8641 gtgaagaatt catgtacaac ggtaaacatg tattgatcat ttttgatgat ctttcaaaac
     8701 aagctgttgc ttatcgtgaa ctttccttgc tacttcgccg tccaccaggt cgtgaagctt
     8761 atcctgggga tgttttctac ttgcactcac gtttattaga acgtgcagca aaattaagtg
     8821 atgatctagg cggcggttca atgactgctt tgccatttgt tgaaacacaa gctggagata
     8881 tctctgctta tatcccaaca aacgttatct caatcactga cggacaaatc ttcttagaaa
     8941 gcgacttgtt ctacgcgggt actcgtccag cggttgatgc cggactatct gtctctcgtg
     9001 tagggggttc tgcgcaaatc aaagcaatga aaaaagttgc tgggacttta cgtctagact
     9061 tagcaagtta tcgtgaatta gaagcattta cacaattcgg ttctgatttg gatgcggcaa
     9121 cacaagcaaa actaaatcgt ggacgccgta cagtagaaat cttgaaacaa aaactgcatg
     9181 caccgctagc tgttgagaaa caagtagtga ttttgtatgc cttgacacat ggattcttgg
     9241 acagtatccc tgtagacagt attttagact tcgaacatga attattcgaa tatctggata
     9301 ccaatcattc agatattttt gaaacaatcc gtacaacaaa agatcttcca gaagaagaaa
     9361 cgttgaacag tgcgattcag gaatataaag acatgttctt agctacaaaa ggaaataatt
     9421 cctctacaga agataaattg aaatccattc aaaatgcata gtgaggtgag atgaatgggt
     9481 gcttcattaa atgaaatcaa gacacgtatc gcttcaacga aaaaaacgag ccaaatcacg
     9541 cgggcaatgc agatggtgtc tgcttctaag ttgacaaaat ctgaagcttc ttcacagaag
     9601 tttcaggttt atgcaacaaa agtaagagaa atcgttacgc atttgactgc tactcagtta
     9661 aatgatattg cctcagaaaa tcctagaggg gatatcaatt acaacagcat gctgctcagc
     9721 cgtcctgtga agaaaaccgg ttatatcgtc atcactgcag atggcggtct ggtcggtgga
     9781 tataacagtt cgatattgaa acaaacgatg tctattttag aagaagacca ccaatcaccc
     9841 gatgattacg tgatgatcgc aattggcgga actggtgcag acttctttaa agcacgcggt
     9901 atcaacctag catatgagct gcgcaattta tctgatcagc caagctttga tgaagtccgc
     9961 aaaatcgtaa atatggcaac aactatgtac caaaacgaag tattcgatga attatacgta
    10021 tgttataacc atcatatcaa ttcgctaact agtcagtttc gtgtagaaaa aatgctgcca
    10081 atctcagact tagatccaga agaagcaacg actttcgaac aagaatatat ttttgaacct
    10141 tcaaaagaag aaatcttgtc tcagttattg cctcagtatg ctgaaagttt gatctacggt
    10201 gcgatcgttg acgccaaaac agcagaacat gctgctggta tgacagcaat gaaaacagca
    10261 actgataatg ctgctaatat cattgatgag ctgacggttt cttataaccg tgcaagacaa
    10321 ggggctatta cccaagaaat taccgaaatt gttgccggag cttcggcact agaatagtat
    10381 tgagattgga ggaaaaaaga tgagttcagg caagattgtt caagtaatcg gtcccgttgt
    10441 cgacgtggaa ttttctttag accaatcctt accagatatc aacaacgcat tagttgttta
    10501 taaaaatgac gaaaataaat caaaagttgt tcttgaagca gcgttagaat taggggacgg
    10561 agtgatccgg accattgcga tggaatcaac agatggttta caaagaggaa tggaagtcat
    10621 tgatacaggc aaagcaatct ctgttcctgt aggtaaagaa acattaggtc gcgtattcaa
    10681 cgtactagga gatacgatcg atttagaaac acctttccca gaagatgcgg aaagaagcga
    10741 aattcataaa aaagcaccag cctttgacga attaagtaca agtacagaaa ttttggaaac
    10801 agggatcaaa gttatcgatt tgcttgcccc atatttaaaa ggtgggaaag tcggactatt
    10861 cggtggtgcc ggtgttggta aaaccgtact gatccaagaa ctgatccata atatcgccca
    10921 agaacatggt ggtatttctg tatttaccgg tgtaggtgaa cgtactcgtg aaggtaatga
    10981 cttgtattat gaaatgaaag attcaggagt tatcgaaaaa acagccatgg tgttcggaca
    11041 aatgaacgaa ccaccaggtg cacgtatgcg tgttgctttg actggtttga cgattgcgga
    11101 atatttccgt gatgtagaag gtcaagatgt actgttgttt atcgacaaca ttttccgttt
    11161 cactcaagct ggatctgaag tatcagcctt gttaggacgg atgccttctg cggttggtta
    11221 tcaaccaaca ttggcaacag aaatgggtca attgcaagaa cgtatcacat ctacgaaaaa
    11281 aggttcgatc acatcgatcc aagcaatcta tgtgccagcc gatgactaca ctgacccggc
    11341 gccggcaaca gcattcgccc atttggatgc gacaaccaac ttggaacgta aattaacaga
    11401 acgagggatt tatccagcgg tagatccatt ggcttcttct tcaagtgctt tagcacctga
    11461 aatcgtggga gaagaacatt ataaagtagc aacagaagtc caacacgtat tgcaacgata
    11521 tcgtgaatta caagatatca ttgctatctt aggtatggat gaattatccg atcaagaaaa
    11581 agtgttagtt tctcgtgccc gcagagtcca attcttcttg tcacaaaact ttaacgtggc
    11641 tgaacaattt actggtcttc caggttcata tgtccctgta gaagaaacag tgaaaggatt
    11701 ccgcgaaatc ttggaaggca aatacgatga tcttcctgaa gaagctttcc gaagtgttgg
    11761 ccgaatcgaa gacgtcgtag aaaaagcgaa aacactaggc tactagaaag gggtgcccca
    11821 tggatcaata tttaacagta aatgtggtaa ctcctgccgg tttggtctat gatcaccatg
    11881 cagctatcgt cgttgcacgt acgacagctg gtgagcttgg gattttgcct aaacatgcac
    11941 caatcatcgt tccattgacg attgatgaag ttcgtgtaaa acgaacagat tcagataccc
    12001 acgtcgattg gatcgctgtt aatggcggaa tcatggaagt tcgtgataat gttgtttcaa
    12061 tcgttgctga tagtgcggaa cgtgaacgtg atatcgatgt atctcgtgcg gaacgtgcaa
    12121 aacaacgcgc agaacgccaa atcgaagaag caaaagcaaa acaagatacg aacgaattga
    12181 aacgggcaac tgttgcgttg caccgagcaa tcaatcgtat caaagtatct aagcactcat
    12241 aaaatgagat gcggatgttt atccgtgttt aagaacagta aaaagaggtt atgacagaag
    12301 tggtcagctt caagacataa gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa
    12361 tctcagctta tttttcgaag aagctacttc tggaacaccg tatatcagag gttgtgaaat
    12421 cagcgttttg tcctgagtat tagagaaaca aatggaaaat agttcctcat attttgccgt
    12481 tttttgagct aataccgagg gtcagatgat tgaacaccgt ttatttggaa taagggactg
    12541 cgacaagact tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa
    12601 atgtaagggg attcctatat ttttaagtat acttgttttt ccttttatac attttatggt
    12661 attatcattt tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat
    12721 acggaattga tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct
    12781 ggtctttacg ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta
    12841 gaatggcaat cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag
    12901 aagttatcgc tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta
    12961 ttttaaaagc aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat
    13021 tttttggagg gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa
    13081 cagtaagaat tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag
    13141 cagaagaagg tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga
    13201 atcaagtgat tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa
    13261 caatcgacgc ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc
    13321 gtgcttcaat cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta
    13381 tgcctggtgg atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag
    13441 ctttaggagc aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga
    13501 tcggtaacac gatttattta gatttcccta gtgtgggtgc tactcaaaat atcatgatgg
    13561 ctgccgtaaa agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg
    13621 ttgatttagc aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa
    13681 caatgcgtat tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc
    13741 gtatcgaagc aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg
    13801 cagatgctat ctctgaacat aactgcccat tgatttctaa attgatcgaa atgggtgcag
    13861 aaatcattga agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag
    13921 atgtaaaaac gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta
    13981 tccaattagt tgcagaaggt acaagtgtgg tgactgaaac agtgtttgaa aatcgtttcc
    14041 aacatttaga agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa
    14101 tcatggatgg aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag
    14161 cagcagcatt agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga
    14221 attatctaga ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg
    14281 tcgaacgtgt agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag
    14341 gaagtggctg aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg
    14401 tgatcttatt gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg
    14461 gtggcggcaa tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt
    14521 ttcattgata aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga
    14581 acatgataaa agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa
    14641 aagaaaagaa ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca
    14701 gcgctttatt tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta
    14761 tatgataaaa gcaattcaaa cccatggtgc gctaaaaggg acggtaatgg ggactgcacg
    14821 gatcttacgc tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattccg
    14881 attaacgcga aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac
    14941 gggtagcact gagcgataaa gaaaaagagg aaatttattg tcaaagtaac aactccgcaa
    15001 ctacttatat gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc
    15061 cttgttatta gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag
    15121 tggctggata tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga
    15181 aaaagaatac gaaatcgtca atgtagcagt aaccccaacg tatcaaggaa aggggattgg
    15241 cggaaaatta cttgagacag cttttcagaa gctttcacag ctagcttcaa accaaactcg
    15301 gatcattata cgtactggca ctactagtag cgctgcgtta cacctctatc aaaaaatggg
    15361 ctatgtggag attggtcgag tcaaagacta ttttatccat cattaatctg agcccatttt
    15421 tgaaaatgga gatcaattaa gggatcaagt gattttagca attcacttac ctaaaaatag
    15481 ctttaactaa taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac
    15541 aatagcttta gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg
    15601 tggatcggct gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt
    15661 tgcaacgcct gaatcttggg aagaaatgaa aacgcaatta tttcaagttt ataaaaaaat
    15721 actcgtttct tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga
    15781 gaaacaggtg attgagggaa tcagtgcgat tccttatatt catggattta atattttccg
    15841 agatttggaa actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg
    15901 aatggctgaa ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc
    15961 aggaactggg attggcgggg cgttgttcac gaaaggacaa ataaataaag gggcacatct
    16021 ttacggtgga gaatttgggt tgatgttctt agatggcgat aagacattca gtaaacttgg
    16081 tacagcggta cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaatgctta
    16141 taccggaaaa gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt
    16201 tgagagtttt tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc
    16261 agaagtgatc gtattaggcg gcggggtttc tgcaaaagaa gggctcattg atgagatcaa
    16321 aagccgaatg aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt
    16381 attatgtgag tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt
    16441 acgtcaagga gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagagaatg
    16501 aaatgaatag gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc
    16561 tgagaggtga aaatgatgtc aaaaaaaaat aaagatattg aagtgcgtat agaagaagtg
    16621 aaaaaagata tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg
    16681 attggtgaga tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat
    16741 tttggaaaag aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg
    16801 cacgaataaa aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta
    16861 ggtattataa ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac
    16921 gcgacggact gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt
    16981 cattggggta tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga
    17041 atccagctac cccagtata
//
LOCUS       DAGCYL010000044        16706 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.045, whole genome shotgun sequence.
ACCESSION   DAGCYL010000044 DAGCYL010000000
VERSION     DAGCYL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.045"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(157..3798)
                     /locus_tag="MB216_001131"
     CDS             complement(157..3798)
                     /locus_tag="MB216_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525979.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(3801..5750)
                     /locus_tag="MB216_001132"
     CDS             complement(3801..5750)
                     /locus_tag="MB216_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBT4525980.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(5792..6850)
                     /locus_tag="MB216_001133"
     CDS             complement(5792..6850)
                     /locus_tag="MB216_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HBT4525981.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(6969..7424)
                     /locus_tag="MB216_001134"
     CDS             complement(6969..7424)
                     /locus_tag="MB216_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525982.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(8008..8811)
                     /locus_tag="MB216_001135"
     CDS             complement(8008..8811)
                     /locus_tag="MB216_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4525983.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            9792..11015
                     /locus_tag="MB216_001136"
     CDS             9792..11015
                     /locus_tag="MB216_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBT4525984.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            11050..11817
                     /locus_tag="MB216_001137"
     CDS             11050..11817
                     /locus_tag="MB216_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4525985.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFKDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            12007..12405
                     /locus_tag="MB216_001138"
     CDS             12007..12405
                     /locus_tag="MB216_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBT4525986.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(12711..13085)
                     /locus_tag="MB216_001139"
     CDS             complement(12711..13085)
                     /locus_tag="MB216_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525987.1"
                     /translation="MKQTQTEPINDTLADQLAGYYKLFEMSKAQRLIHYLWKYKILFL
                     FWEATLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(13107..13484)
                     /locus_tag="MB216_001140"
     CDS             complement(13107..13484)
                     /locus_tag="MB216_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525988.1"
                     /translation="MKSKKETEPMKSTHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(13474..13869)
                     /locus_tag="MB216_001141"
     CDS             complement(13474..13869)
                     /locus_tag="MB216_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBT4525989.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEDLLEK"
     gene            complement(13936..14121)
                     /locus_tag="MB216_001142"
     CDS             complement(13936..14121)
                     /locus_tag="MB216_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBT4525990.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(14203..14457)
                     /locus_tag="MB216_001143"
     CDS             complement(14203..14457)
                     /locus_tag="MB216_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525991.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(14731..15015)
                     /locus_tag="MB216_001144"
     CDS             complement(14731..15015)
                     /locus_tag="MB216_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525992.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(15509..16048)
                     /locus_tag="MB216_001145"
     CDS             complement(15509..16048)
                     /locus_tag="MB216_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525993.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKQQLDRIKEENNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            complement(16161..16427)
                     /locus_tag="MB216_001146"
     CDS             complement(16161..16427)
                     /locus_tag="MB216_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBT4525994.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(16429..16650)
                     /locus_tag="MB216_001147"
     CDS             complement(16429..16650)
                     /locus_tag="MB216_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBT4525995.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
BASE COUNT         5150 a         3107 c         2571 g         5878 t
ORIGIN      
        1 gcataagccc tttttaatta tacaactttg aacagtaaat atttaattca tttatagaca
       61 atttaaaata atttttttgc ataaatagaa attttccttg acatcttcag ttagaaatca
      121 aagtaaaaag aaggagcaaa tcaatgctcc ttctttttac tcgtcttcag acttattttc
      181 agttgatttt tgattaccgt attttacaat atagtccttg actgacatgg tagatttctt
      241 cactacttta ccagttttgg atgctcctgt agcagtagcc ttggcaacct tagccgttcc
      301 tttacctatt ttaatagctt cgtttttagc ttcataggtt atccctgcta gactaccttt
      361 tggagctttt gctaaagctt tatctgctga ggtgtaaaca tttttaccag tttttgatag
      421 atattcaacg gctgatttag ctgttgatgt tttcttggtt gattgattag atttccctga
      481 attatctgaa gtagatttag aattatttgt tctattattt ttatttgtca gttcaggttc
      541 tctaattgat tgattgtctt tcgacattcc tgatccaata tttgtttcat atttatctgt
      601 ttgattaaca ttaggagtag gaatgaactt tttcttcaca tctgattgtt ctttatccgt
      661 ttcttttgtt agcgattctc tgtctttaag tactgtgtca ttccgaatag cactattatc
      721 aaagttgctg ttaagctctc ttgactgact ttcaccagat gctccatctc ctaattctcg
      781 atgcactctg tctgacacgt cctcaccact catttcattc ctcagttctt gatgagtttg
      841 atctaaagta atctcgtctt cttcattctc aggaattgca acagcaattg gcgtatgccg
      901 ttgttgttcg agttctctat ttcgatctgc cacaagcgca tctctcgtct caatgatatt
      961 cccatcctca tcgactaaat ctccattcac attatatcca tcgctgtgtt taccaagttt
     1021 attaccaaac tcatcaacaa gatttccatc ttcatcaact aaacgacctt tttcgtcaac
     1081 taaacgtccg ttactatcat gaccaaaatc accatttagc aacctgcgtc cattttcgtc
     1141 aacaagattt ccatcttcat cgactaagcg acctttttca tcaattaaac gtccttcgct
     1201 atccatacca atattaccat cttcactatc ttccaatggt ttaccattag tatctataag
     1261 gtttccatct tcatcgacca aacgcccttt ttcgtcaata aagttgcctt tagcatcttt
     1321 accgtattta ctatctttta atatttcacc attttcatcg acaaagtttc cgttttcatc
     1381 aaccatacgt cctaaatgat caatatattg ttgggagtta ttacgtagtt gatcttgacg
     1441 aactaaatct ttgaaagagg tattattgat tgtggaacca actccgccac cgttgaatga
     1501 cattccttct ggtgtttcag gaattacttt atcagcaatg gacttagcat cagcattgct
     1561 atctcccata ttgttcaaac gttcagccat tttttgcgct ccaactgctc caagacctcc
     1621 tgcaaccata tcttttactg agctgttgcc accatcataa tagggatttt tagctccttg
     1681 gcttttcttg attgctttag aaacgccgtc cacaactcgc acagcatcat ttctattacc
     1741 aaataataac ttagcacgtt catcaaaccc aaacgctact tcaagtggta agccaataag
     1801 catattaatc aaattattta ctttttcgcc ttttttaaag actaaaagat atcctacacc
     1861 taaaatcata gcagatcgaa caatatttgc tacgccatcg ccaactaatg tattaggaat
     1921 gaatcccaca gcagtatcac caaatgcacg aataaaagta atcagaaaga taggtaattg
     1981 tagtccgatc agagatgttg taacaacacc taatgaaatt gccatcacac ctgctcctga
     2041 tgcaagtgat cctgttagaa ctacttccca aatccgaata aattcacgtt taaatgcttt
     2101 cacgatattc acattgaaca tcgcccaagc acatgctaag taaagaactg ctaaaacgat
     2161 tgattcagcg ttcatcgcta gccacgagcc aaacttaccc atagctccat gaccaactaa
     2221 ggatgctgta cgccattctg tttttactgt tgacatattc ttagcactgc cttgaattcc
     2281 tgaaggagcg atattatacg cataaaactt agctcccgat gtatcaagtg ccgattggag
     2341 caataaagct accgactgtg tgctaaaagt tgcattgtta gcaacgcccc aaggttgagt
     2401 agcatcaaag ttatgcgctg ttccttttcc atcaggcttg cgattgtcgt ctgtcacatt
     2461 ttgatttgct gaccaaatat atccatttaa attggttggt ttcttaattg cacttccttc
     2521 ttttccatcc cctaaattat agcctgtcca aacagcaggc gctcttccca tataagcatc
     2581 attttcggcg acattaacat caccggcata agtattaata ttgaaagtat cattcgatga
     2641 ccattctttt aatagagcca taccgactga attagaatct agatcattat tttgtctggc
     2701 ataactgcta gtattgatcg ctcttatcat tgcagatttt ttagatggtt cataatctgt
     2761 atcaacgaac ttgatttcgg atgtaggctt tccgtcaggt acatcaccac cagtatcaga
     2821 tgatggagca agattttgag tagaagccca aagtcttgtg ttaatgatgt agtcagaaac
     2881 aacatttgtt ggtatatctc catctttttt tgctgactgg atgccgttga tataaactcc
     2941 caatagcgat cctgccactg gtaagaatat aaacaataca aaaatacgaa caatccattt
     3001 ttcaagtact ggtgataatc ccttcgagtt aacaccatct ttcgacaata atttcattcc
     3061 ttgataacct agtattgctg tgaaaaagag catagataag cttgctatcg ctttaaataa
     3121 ttcattattt aatcccaaat tgttaagcaa gctagttaat gctttagata ccggattaga
     3181 tacatccgat ccaccttctc cccaccccat aagagaaaat atattcatac tcatgaaaaa
     3241 ttttccaagg agttcattta tggattcagc aatattagaa aggattaaac taattgatcc
     3301 gataaaacta ccaatcgtcc taccaatact tcctagaatg gatgatgctt gactggcaat
     3361 agcatttcct gagactgttt caatatatcc gtagtaacta taagttgcta aagtagatgc
     3421 tagttgagta cctgcattac cccctgcaat cgaattacct tgtttagaaa tgtcagaata
     3481 agggaaatcg gcactgatac caccttgtcc taacaaattc atttgtccag ttttagcggc
     3541 actatcatta acttctttat ctcccattag gatacgagca tatagtgtcc acattccttg
     3601 ttctgccggt ttactttccg catatgcaac gttcttattt ataactgttt cttgtttgct
     3661 atcttgattg tcaggagttg gatcattcat tccaaaagga gcgcctaaag taactgctaa
     3721 tactcctcca ataactagac ctcttacgac ccatcgtttt attctaaggt tttgtagtaa
     3781 atatttcttg aatttcatag ctacgcccct ccaatcgaaa atacaatctt gttttcttta
     3841 ctttcagcaa cggaaggctt tgcaataatt tcagcattag aatcaccttt tccttcaaac
     3901 gctccggctt cactcaactt aacatcgaat atttttgcgt ttggataagt aattattgct
     3961 tcagtaatcg cttcttcaaa ctcagattca actaaatgca agatattcct tgcaccccct
     4021 gatttagaat cctcttgtga agccatgtca aaaactaagt gcatggagag atcgtctgca
     4081 taaaaatttt gaccatatac tctttctttc ctaggtggaa gacgtaaatc tatatttaaa
     4141 tgttttcgta ctaattcagc actcttcaac atttcattat aagcaatttc cagaagagtt
     4201 tgttcggata aagcccaaaa tggaactatt cgtccaaatc ggttaataac ttcaggcttt
     4261 agaccttcac caatcaaggc ttgtttgatc cctgatgcga cttttcggta ttctcttttt
     4321 agttcagtgc tatattcatt agaattttcc ttgtttgtaa cacgagatag tgagctatat
     4381 ctgtttaacg aatcgaacat ctccgcacct gcatttgttg tcgcaaataa aattgtattt
     4441 gacaatgaaa cagtgtcgtg aacttctcga tcatcccatc ctctttcagt aaaacttatc
     4501 ctaccttcat ctaaaattga taaaaaggca tcgagtgttt ctacaccagc tttctcatat
     4561 tcatcacaca caataatcgc agaaggatca tgccttacat gagtacctat gagtcgtcta
     4621 aaacgcattg catcatcttt tgttttgtag tccggcatat taactaagag aactttattt
     4681 tcatcgccat aaagtcctct tgccacggct ttagccattg cagttttacc gacccctgtt
     4741 gatcctgtaa acagagcagt tgcttttggt tgctttcgac ctttaaaacg atcaaatcca
     4801 agcttcatac gtttaaacaa cattgttata gtataaactg ctaacggttg cccttttatg
     4861 gactttaatt cctcttgaag tttttccata tcaaaatcag aatctagctg aatattatac
     4921 ttaccaaata tttgcaatag actttctcga ttataagcat ttccgtctac tcgatctttt
     4981 gcaatcaaat cctccattac ggcaattgat ttagccggtt cggacaaatc ctcacgataa
     5041 agcttgtttg cttgtacgat ttcttgaagt aattcatcag gaatcctttg cgataattta
     5101 aaagaagcct gaggatcatc tttaacagag gatgtattta gccattgtct caaaatatcg
     5161 acgacaacat cgctttttag ttcattcaat tctaacggtt gtaaccgtct ccctaaggca
     5221 tcatctcttt tgatatagtt ttcatattct tttcttgtag tagcggtaat caattgaagt
     5281 gattcatttg ctaacggttc ttttagcgaa tcacccccaa ttttattgcc actaccaaaa
     5341 gagctaacaa ctttatgaaa ctcgtcaatg aagaggacaa attcattttc tttgtcctca
     5401 gattttaaaa ttcgctcata ttctttcaat cttggaatta atgtttctaa acgtgtttgg
     5461 aggacatcat ctcccccaac acgcaattgt ccgattgata atttgtatac ttgtactttc
     5521 tttccatctt gtcgctgtct ttcagcccat tcacttgcaa gtgctgtttt acctgttccg
     5581 gcttcaccta acaagatagc ttgcggtgtt tccggtctat tcaagatcag tgacaactca
     5641 tggagttcgt catcacgtcc cactaactta tgtttcggtg gtttcattgg tgtgagagca
     5701 tgattaaatt cagtttgaat attcttaaaa aactcggtac tattttccat ctgtttgtgt
     5761 tcacttcctc taaaataaga ataatttact tctagtcagt cagagttagg tacttaaatg
     5821 tcgtcttcat gtctccaaaa gaatctttgt taaagtcagc atttttgtat tgatcaatca
     5881 atttttgtgt atcattgatt gctttaattt cggtctccat tcgactgtct agttgacgaa
     5941 tactttcttg cgaatcttta tcgtcaggat tttgttgtaa acgttccaga tactcagctt
     6001 ttgtagcttc aattttcttg atgttagtat ttgaagcatt taactgatct tctaacttac
     6061 ttacaacatt attcttactt gtaactgcta caacttttga ataatcaata tcgccatctt
     6121 tggtaagaaa gcttccttga tagacattag ttgaatctga atatggattt attctgaaat
     6181 tgatattatc aggagtatta acaggttttg cttttttccc aaaagaaatt actccttcat
     6241 tattaacatc attcatagaa tattgagaaa gtgcttttgt aactgaatct gcttcattca
     6301 cattgacttt ggtatcatcg tcagctgtat catcaccaga aattagtgat acttggtttg
     6361 caataaaaac ttgataagct ctttcatcta atttaccttt taggaataag aaagcatccc
     6421 catcagtact taacatccca tagctcattt caagcttttt aggacgatct ttttctttcg
     6481 ttgccatata taagttgtag ttttttcctt gcgtagataa caactttcta gcgttttgcg
     6541 agtaaccgag ttttactact gttacatctc tgttttgatc tgtccacaca tctgaaatag
     6601 tgatctctgc tcctgatcga gcaaactcca atgaagagcc tataggtgtt gtattgttca
     6661 cgagataata attatagttt cctaattgat aaatgtagct acccaagcct gttaataaaa
     6721 aaagcactcc tagaaaagtt gcaagcataa tgcgtaatcc gtgacgtgtt ctcttatcct
     6781 tatcaccaag caaccctcca ataatactgt attgatcttt ggtagatgtt gaattgacgt
     6841 ttttttccat tagtttgtct gccccctttc ataaaagagc aaacaatcag acgttttttc
     6901 ggtaattcta ggttcgggat tttgaatcat ttcggtatat tcttccccat taatatttaa
     6961 cacaaaaatt atcttccttt cttcgttcct ttcttttgct ctttattttc caaataatca
     7021 atgatttttg ctattgtacc aagatagtaa ttcccctcgg ctttgttttt tcggattttg
     7081 ctaaccattg gagtaggtac aatctcttca tctctcaatt cactgaaaga ttttttacct
     7141 agatattttc caataacaga ttttaaaaaa cgttcaaaat ctttctcgta agttgttttc
     7201 ttgcctttta gttcatggtt catattgtca acgacttttg cggtttcaat aattgtttta
     7261 gttctcagct taataatttc atctggaatt ttcaatcctc gtaatttcat cgttgtacta
     7321 actggtaacc catgctttac tgcatcagca atactaaaac tttttagatc atctagtaag
     7381 ctctccatat agttaacaaa ttcatgccaa ttaatagcat tcattgtctc accccttcat
     7441 ttttttaaaa tgaggttcgg gcgtaacaca tttatgttat tataatatca taataacata
     7501 aataaatcaa tcagcaaatt tagatatccc ttaaagtatc agcaattatc caacgagata
     7561 tttattttag ctatttcagt catcatgaaa gactaattag caataaaata actaatatta
     7621 tccatttatc agacgaaaag aaaaaactta tctaattact ctatattaac ttttaacaaa
     7681 tagttgactt gtccaaaaat gttaccttca atctcacaaa gatattaaga tgtttcctac
     7741 caaaactgat atcaccggac tttttattct gtgttgtcca aaagtgttac ctttagtcga
     7801 tcaacaaatt aagaaaaatt gtttttttct tgacttgtcc aaaagtgtta cccatagttg
     7861 ttaaaatttg tatagattag ccattctttc aaatattatt tagactgaaa cttgcttaca
     7921 aaaaaacttc ttgcatttat tgtccaaaag tgttacccat acttttttaa agaagcaggt
     7981 tgtattaaaa ttacaacctg cttcttctca atcttcttct aaccaattat gcattgaaat
     8041 ggttggcaag tttgattctg gctcactaaa agtgaatctg agccgatcaa ctcttcttcc
     8101 tttctttgca tagatttttt cataatcaaa agtttcaaat acctgtattc cgttttcatc
     8161 aggagctaaa aattcttctt tcgcctgctc tagtattctt ttatcaatat ttgtcattct
     8221 ataacttgaa ggtatactta atgctcttct aaaatcttca actttgacta cccaataacc
     8281 atttcgccac tctttatttc tgtattgcat gagatgacga tacaattctt ttgtgtagct
     8341 acttcttagt tttgtaaaat tatcaagttc aaaacgagta aagtttttgc caattgagtt
     8401 taagataaat tcaaattcct catttgcctt aattgtaact gtttccttct gtttatctat
     8461 ccggaatttt tgaaaaagcc acagctgttc atagacgtct gaatcttcaa aacggtaggt
     8521 taaattacct agtttgtcac tcaaatcttt taacacagaa taaaaactgt ccttttctct
     8581 tctgtcgtat ttgaccaaat aacgaaagag gttgaaatca aaagttactt ctgctgttcc
     8641 ttgacgtttc atttttgata aaatagccca aaacacatcc atttcaacag gcttaaatcc
     8701 tcgcaaagct gttgtattca gaccattgtt ataacgcaca attgcatttg tttgttctga
     8761 tactaaatct acatcactat caataatctt ttcggtaact tcttgagaca ttgattactc
     8821 tccaatcttc tttttgcgct cctttaatca tgttaatctg gtactaatta aaggaaacaa
     8881 ttttttctgc ttgttaccta aattaaatca caactaaagg taacagtcaa caaagatttg
     8941 ttactaatct aattttctat tttttcgcct tccaaatcaa gaaaaccgaa ggtaacgtct
     9001 tatcctagtg tgatacctca aatgtccact tttgttacct ttgctgtcca aaactgttac
     9061 ccttactgtc cacttttgtt acctttactg tccaaaagtg ttacctttat acccctttaa
     9121 tacttactct cccaagagat taagcctccc ctaacagata tattaacaga tatttaacag
     9181 atatattaac agatatttaa taaaggagga ttactcctct cttatttcaa aatcttaaaa
     9241 ataaaaaatc ttaaaaaaaa ctaacactat ctaggagttt attttgcttg tgtaactttg
     9301 aataattgaa aaaaaggaaa aggaggaagc ttcagcttcc ccttttattt atatttataa
     9361 tatatatttt aatctttaaa actttagcaa cctccaaaaa ggctttatat caatcttaaa
     9421 tagattttag ggtggacata aagcgatgcg aacatggaca aaaagcgatg tgaatgtgga
     9481 caaaaagcga tgcaaacgtg gacaaaaagc gatgtggtat tccgaatata tccaaaaaaa
     9541 gaaaaagtat gtgaatttat ttctaaaaac tagaaaaata tttgtttttt aattttggta
     9601 atttttataa tttcgtattt tattgaaaag gatttttatt ttgaaaatgt tctaaaatag
     9661 cgatttctgc tgtattttca atgaaatttg attggaaaaa ataaaatgtg ttttactgac
     9721 attgagttat gttataaatt tacgatacat ttcttattgt aacgtatcga aaacaggtct
     9781 atacttattc aatggaatcg taccagttcc aagtttttaa gctcgtacca agcttagaaa
     9841 gaagaggcaa tcgtatgaaa ataacagaaa agaatgtaaa cccgaaattt gaaactaccg
     9901 aaagcaatct aaaaaagttt gatttaactg ttactttatc gaatcaactt gtagatagca
     9961 ttgttgaaat ggatgctatg ccacaaaaaa tttatcttta cgctatagga catcttgacc
    10021 ctaaaaatcc gccttttaat aataccgtca aagtatctaa aaaggcttta tttgcgttga
    10081 tgtcttctag ttcatcttct tcaaaacatt caagattaaa acaagttatt gaaaaattgc
    10141 atcagcaatc tgtcattaaa attcaaagaa ctaaacctaa cggtaagact gaatacaaag
    10201 tcatctcacc gctagaaatt actgaattca atgatttttc tgatgcagta tctattaaat
    10261 ttagtagttc aattatgcct ttattaacga acttagaaga tcattttacg caatatacat
    10321 ttgatcaagt agttaacttg acaagcaagt atgcgatcag tttttttcaa ttattcacta
    10381 aagattttaa tctgtatgaa gcaaatagag ataacttaac atggagtaca aaatcacttg
    10441 atagattaaa aaatcctgtt ttctccattg cagatttacg aaagaaattt agtattgaga
    10501 aaaaatataa agcttttcga gactttgaaa gacgtgtaat taaagaaccg attgcccaac
    10561 tgaataaata cactgatttt aaaattgact atgagcttat caagaagggt catacttata
    10621 caaacataaa gttcttgctt acacgtacca tgtcagttcc ggtaccttat aagcaagaac
    10681 aaagagaccc tgcttattta aaaggcgcag aagataaaac agagaaaatg aatcaatcgt
    10741 acttgaaagc tatgaatagc aaatacacta aattactaat caaaaacttt ttgatcagtt
    10801 ctgccgactt atgcgatctt aaacttatga gccgacttca gaataaagtt tatccgcttt
    10861 atgatcgttt atccaaagat agaggatttg aatcagttgt caatcaccta tcttacgttg
    10921 ctactcataa ggaggattac acaataaaaa atactgttaa atatctcgaa aaatcaatta
    10981 cgagttattt gccttatgtg aacctttatg attaacaact gaaaaaatgc gtgaaggaag
    11041 gaaaaacttt tgaaaaaaga tgaactgaca aagtacaatg aggagttctt agcaacaatt
    11101 gatatgaaaa tggatttaaa gtcgtatgaa ctttatcaat tgctgttcag ttatatctca
    11161 cccacaaagc taaagaaaac aggcgtagta gatttacctg ttaaagcgat tattaattac
    11221 atggatgttg atcaaaatct tgaaacggaa ctaaaaacca tagttaacac tttgcaaaga
    11281 caagcagttt atgaaatatc gaatctagac aagaaatata agacgtatac tactgtatct
    11341 gctattgatc ggattcattg ggatttagaa gagcatagtg tatctttcag tttttcagaa
    11401 gatgtaattc ccttaattgt ctcaatcaaa aagaagtatc cgttctttgt ttttaaagac
    11461 ttagcatctt ttcaaaaaaa atatagcatc gtgttataca aatatttggc gatgtattat
    11521 aaaaaatatc ttatcgccaa aaataaagga gattttgaga aagctgaaat atataagaat
    11581 cccctgattg aaatagttaa aattcgtgag attacgaaaa cagaaaatga gtataaaaca
    11641 atatcgaatt ttgatagacg tgttttaagg gaatcagttg aagaaattaa tgaaaaatca
    11701 atgctgaaga ttgattttgc taaagttaga aaaggcagaa attacaattt tgctgtccag
    11761 tttaatattt ctagtgagtt tgatgaaaaa ccttatagag aaaaaaaatt aggttaatgc
    11821 aaaatatatc ttactcgttt gaagatgctt cagacgtatg ttaagatatg tagtgccttg
    11881 ggagcaaggt attaatctcc tttagaggtt aattagcttg tgatcgccta tcaatttttt
    11941 ggtaggcgat tctttttttg aaaccaaaaa gcaaaaatta ttttttaatt agagaggtgc
    12001 aatgcgatga aatacttttt ttgtgcagta ttcgaatgga aaggaaatga atatgcagtt
    12061 aaattcccag attttccaga cttatatacg catggtgaaa ctttagaaga tgcttttgag
    12121 atggcgcaag aagtattgga aggatacttg gtgcttttag agttgcaaga aaaagaaatt
    12181 ccatcgccta cgcctgttca agagttaaat gcaagaatca agcgatctga gaatgttaat
    12241 ttatttgtcg taaaagctaa tacgaaagtt gctaaagctc gaaacaagca aaattatgat
    12301 cgaaaatcag tgactgtgga acactattta aatgttttag cggctgaaga gaatattaat
    12361 ctgtcgaaag ctttgaatga tttgttgaga gaaaagttga tttgatgtct ctcagatgat
    12421 catattaagc aaaaacagct tgttttagga cgtatggatt taagaaagac aaattatcaa
    12481 agaatatatt aaaagctctc agaagcgttc tgagagcttt taaattgcta tgtattatac
    12541 tgcatctgct caaattgatt ttcttttcat gcttaccatg tatctagttt tccatcaagt
    12601 aatttttaga tatgtattga ggaataaata gcaaatttgc ttgatataag ctcgaattag
    12661 gtgctttgta atgtgttagg ataaattata caagaaaatg tcaaaagctc tcagaacgct
    12721 tctgagagct ttttatcggt aggcttgttt aacgtgatct tgaaagatac ccttttgatg
    12781 tttctctcga tccctgcatc gctaattact aagtgttctt caagtgtgat gtaggtatat
    12841 aaattaagca tcaaaatggt gtagataagc aaaaaactcc ctaaaactaa gtgttgatta
    12901 taaaaatctg acttataaaa aagaactgag aatgttagtg ttgcttccca aaaaagaaac
    12961 aaaattttgt atttccaaag atagtggatc aagcgctgtg ctttactcat ctcaaataat
    13021 ttataataac ctgctaattg atcagcaagc gtatcgttaa tgggttctgt ttgtgtttgt
    13081 ttcatctttc tcttctcctg tttcaattaa tctaaaattt cagattgagc aagcaactca
    13141 ttgtggtagt cggtttgaaa ttcaaataac aatttagcct tcttaacgag gtttaaagcg
    13201 ttcgttagtg attcacttaa tgcttcaaca tctttaactg attttgcatg tttcattttt
    13261 tcagtgtgcg tatagcagaa ttcaggtatt gagttaggag ttgcctgttc aataaacatc
    13321 tcaattcgca agttattatc ctcaaaaata attagatcat tggcaaagtg tttggtacta
    13381 acgctaatgc cagttgtatc ttttaataga ttattgatta acgttcgaga gaaatagttt
    13441 aagtgtgtag atttcatagg ttcggtctcc tttttacttt tcaagcaagt cttcttctag
    13501 tgctttttga agtaattgag agaagtttat gtttttctcc ttccctaaaa gaactaacca
    13561 ttccggaaca ctaacatttt ttctaattgt ttttgtattt aagcttcttc ggaaaagttt
    13621 aatgtttagt tggatgatta ctactacttg attggctttc aaagttaatt cttccactgc
    13681 tgatggttta ggaaattctg catctggtac actatatagc gctaatccta aagcatcctt
    13741 agcgtttaaa tatgcttctt caagactatt tccaaaggta gatatttccg gtaagtcagg
    13801 gaaaacgatg ttataagctt ctccttcttt ctcgaagatt gccggataaa aaatattttt
    13861 tgtcttcatt tcctcgctcc ttttctagtg taagagagga tagtatgcct aataccaaat
    13921 aggaaggcaa ggctgttaaa gccctgcttg cttccagata ttagcttctg ttcctagtgg
    13981 tacatcacca ttgtggatcg gaacaacgac cgttctttta gtcttggaat tataaaattt
    14041 tgcatgactg ccagtttgtc cagtcttttt gaagccgtta gatttgagaa tttttacaat
    14101 ttctcttgct gtcttaggca tagctaccct ccctttacaa aatctattat acacattgtt
    14161 gtgtatggaa tcaatagaaa aagaggatgt tttattgcaa aactaataaa atgatttatt
    14221 ggttttttca gcaaattgaa tgttattagc gttgcaaata ttcataattt cccaagtatc
    14281 attagaagta taggtatcta agaattcatt gacagttacg gttgattcgt atggttcata
    14341 agtcaaagcg ttctccaagt cttttgatga aatgatcaag actttttcaa tttcctcccc
    14401 tttttcattt gtgaaaacgt attccaaagt gatttgttct tcatcggtat aattcatatt
    14461 gtgttctcct ttcttgtatt aagaaaaatg gatatttcca attgcatccc actcggcacc
    14521 attgtggtat aagacgtgac cgagcgttac gcctttgaaa tgctcttcta aatacatcta
    14581 aacgcattca aggctagtac ctgctttgag attgtagaag tctgcatcca ataccttttc
    14641 agaagttatc ggatcaatgc gagttggtac ctcaataaat tcagtccaaa tttctgcaaa
    14701 agtcttacct tccaaagtcc acactcccta tcaattaatt ccaaaagcaa atagagaaat
    14761 caagcgaccg tcaatagtca cttcaacaac tgaaaatgcc gttgaaacag ttttaacatc
    14821 cacactttcc aatttcccta tgaaggaatt tttgtgcttt agaatagctt tctttaactg
    14881 tctgttatac gcttgacgac tgttaaactt tttgaattct cgatccttga tcagctgttt
    14941 aacgacccct gcattaattg tatagctttc tgcaatagca acgtgagtag gttgctgtaa
    15001 tgtagctgtt agcatattgt cactccttcc taaaagttaa ttagcttgtt tacaattaca
    15061 ttatacacac tagtgtgtat aatgtaaaga gaggtttttt gctgatggat cagctgtgct
    15121 aaacaaaaga aaacgataga gtaacaaaaa aagtcagcaa agttaaaaag gaggaaacgg
    15181 ctagtccggt tgctttgctt gtgtaagttt cggctttctt atagatcaaa taggaaatta
    15241 tcagtgatat tccagtaaag aataatgtac tagctgtcac taaaagaaat aggttaatca
    15301 tacgatacac tccttctgta atattggtat cagatacgtt taaatgcgtt tctgagctgt
    15361 tttggatcaa tggatgtttt agccctaccc taaaatcaaa gcccagaaaa gcatttaaaa
    15421 acgttcgtac ctaatacgtt tatattcata tacatataca taaacataaa cataaacgta
    15481 tacacgttta tgtttatgta tttaagtttt aggctacttc cttaacatct cctgctgata
    15541 gtcgagtgaa ccgagtgagt gatccatctt gtagtctaaa aataaagcta cgtttgagtt
    15601 tatactgata aacagcgaca tacgatccat cccagattga gtaagtttta cctgcaacta
    15661 ataagtcatc aaggtttggt ttattgtttt cttctttaat tctatctagt tgttgcttaa
    15721 ccattttttc ttgatcaccg acaaagaatt tcaaccattt tttcggaatt ttcttaaagt
    15781 aagcaggact gtcatttaag aagtagattg gtttgtacat ttcatctcga aagatgaagc
    15841 tgtcaatatc gtaggttcct tctgcatctt tgaaaaggat aataccatag gtcttcgtta
    15901 ctttggtatc tacgatttcc aaaccatttt tggcgtaccc tttcttttct tcgatcactt
    15961 catttgctga tgaataagca ccataccatc ccataaaaaa tcgctccttt tgaaagttaa
    16021 ttagcttgtt tacacttata ttatacacac cagtgtgtat aataacaaga gggtattgtt
    16081 cttttttagg taaagtggga tcgtaaacag tgattttgtt gggttaacaa aaaaacagac
    16141 aagccttatc agcttgtctg ttacttataa acttcacttc tgtgaccaat agacaatata
    16201 agaattgtca cttcatgctc gctaatatca gctattatac ggtaggcacc taccctatat
    16261 cgccagtagc ctgatttatt agctgttaga cctttaccat gttttcttgg atcatcagtg
    16321 cctacaagat ttttctcgat ccagtttagg ataacctttg cctgaaactt atccattttt
    16381 tttagcgctt tttgagcttc acgttcatat cttacttggt aagtcatctt ataagcctaa
    16441 ctcttttagc atatcttcgt gggaaatact ttgatcgttt tcctcatgct ctttcattag
    16501 ttccttgtag gtggcaaagt ctactaacgt ttctgctgtt tgcagggaat tactacgcat
    16561 aaattcagaa atgttatctc cgttgaattc agcaactttt tcaagaaaat ctttttcttc
    16621 tttagataca cgaatactta ttactggcat agtttatcaa ctccttttca attacattgt
    16681 actacgattg tatgacaaat gcaata
//
LOCUS       DAGCYL010000045        16683 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.046, whole genome shotgun sequence.
ACCESSION   DAGCYL010000045 DAGCYL010000000
VERSION     DAGCYL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.046"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..546
                     /locus_tag="MB216_001148"
     CDS             <1..546
                     /locus_tag="MB216_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBT4525996.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="MB216_001149"
     CDS             735..1403
                     /locus_tag="MB216_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBT4525997.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1989)
                     /locus_tag="MB216_001150"
     CDS             complement(1603..1989)
                     /locus_tag="MB216_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4525998.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1961..3343)
                     /locus_tag="MB216_001151"
     CDS             complement(1961..3343)
                     /locus_tag="MB216_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBT4525999.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3499..4686)
                     /locus_tag="MB216_001152"
     CDS             complement(3499..4686)
                     /locus_tag="MB216_001152"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBT4526000.1"
                     /translation="MEIKRSLNTKFAEIFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4693..5724)
                     /locus_tag="MB216_001153"
     CDS             complement(4693..5724)
                     /locus_tag="MB216_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBT4526001.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5770..7194)
                     /locus_tag="MB216_001154"
     CDS             complement(5770..7194)
                     /locus_tag="MB216_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4526002.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            7369..8274
                     /locus_tag="MB216_001155"
     CDS             7369..8274
                     /locus_tag="MB216_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBT4526003.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8365..9042
                     /locus_tag="MB216_001156"
     CDS             8365..9042
                     /locus_tag="MB216_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBT4526004.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9039..9209
                     /locus_tag="MB216_001157"
     CDS             9039..9209
                     /locus_tag="MB216_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526005.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            9323..10219
                     /locus_tag="MB216_001158"
     CDS             9323..10219
                     /locus_tag="MB216_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4526006.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10274..11644)
                     /locus_tag="MB216_001159"
     CDS             complement(10274..11644)
                     /locus_tag="MB216_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4526007.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11882..12628
                     /locus_tag="MB216_001160"
     CDS             11882..12628
                     /locus_tag="MB216_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBT4526008.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12625..13464
                     /locus_tag="MB216_001161"
     CDS             12625..13464
                     /locus_tag="MB216_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBT4526009.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13465..14415
                     /locus_tag="MB216_001162"
     CDS             13465..14415
                     /locus_tag="MB216_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBT4526010.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14556..15308
                     /locus_tag="MB216_001163"
     CDS             14556..15308
                     /locus_tag="MB216_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526011.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15327..16478
                     /gene="queG"
                     /locus_tag="MB216_001164"
     CDS             15327..16478
                     /gene="queG"
                     /locus_tag="MB216_001164"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBT4526012.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5431 a         3105 c         3339 g         4808 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttttcttga aaatgccaaa catgttatcc
     1141 tagagaatgc cggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt tgataccgtt gactagttct tccatcgcaa aagcagtccc
     1921 ccattttaat ccaaatggcc gatctcgata ctcgttatag tcaatgaatt tttttgctgt
     1981 ccgtctcatc ttttccacct tctaatccac tcataccacc tgcatgcccg ccaacgagag
     2041 aactacgtgc gatggcgcgt cctccttgca agagactatg cgcatggatg attgattgaa
     2101 agccgtattt ttttctaatc gtatcgacaa caaaatcaac tttcgcttca tttatctgct
     2161 tttcaggatc ttcaaaaagg ttaagctgta aacctgtcgc gtatacgagg tcggaacagt
     2221 tcacgcctac acgccgaata tcctggtagt ggtaatgctg gtggaaaatg gtcaataaag
     2281 catctgctag atctctgcta gcatttgtcg cttcgatttt catttgctgg tgaaacccgg
     2341 tcttgccatt tggatcaaga tagcctaaag agtatcctac ccataagctg actacttgtg
     2401 tttttgctgc agatcgtctc aagcgagtgc cgacttggtc ggccatttct ttgataacga
     2461 tctcgatttc ttctcgtctt gtataatctc tatctaggat ttgactattt ccgatacttt
     2521 ttgattttgg tgtatattct tgtcccaaaa aacttcgatc gatgccccaa gcatgggcat
     2581 aaagctgagt accgatgacc cctagcttgc ttttcaagtg ataataatcc gcgtgagcta
     2641 aatcataaac agaatgtata cccatttgtt tcagacgttt tgctgttcgt ttgccgattc
     2701 cccaaaagtc agtgatatct gagattttcc ataacttttc tggtacatct tcataacgcc
     2761 attcagcaaa catttgcttt ccgtcttttg agccattgtc taaggctagt ttagcaagta
     2821 gcgggttatc gccaattcct atagtcgtgt agattccggt ctgctcaaac acatctttat
     2881 ggatcagttt agccatgtcg tacgcatttg ttttaccaaa gagtgccatg ctatttgtta
     2941 catcaaggaa gctttcatcg acggaataga catgatggtt cgcttcgtct gcatattttt
     3001 tatagataga attgatttct ctgttctttt ccatataata ggccatcctt ggggcaacaa
     3061 tatataaatc acttggatag gggaagggaa gatctctggc acggctgata ttggtgatgc
     3121 cgtaagcttt ttttgctgca ggactggaag ccaagattaa tccgctaccc cgtgtttctg
     3181 tcgaatcgga cggataggac atgactacta atttagttgt aaggggatca agatcctttt
     3241 caacgcactc tacggaggcg taaaaagatt tgcagtcaat acataaaata tctcttgaag
     3301 gttctttttc ataaacaaat accggatttt gattcgccca cataacaatc acctcttcaa
     3361 cacaattaga acacacgttc tctttccgtg tcaataaaaa acagcaattt tcaaatacga
     3421 gcaaaaggct cacatttgga aattgctgag aggttatttt tatctatttg tttttagctg
     3481 agatcgttga tttttttatc aaacatgaac agcttctgga tcgatctttg ttgtcccatt
     3541 tccaatgtgt gcaacataaa attccggagc atagccgaca actttcatgt attcatcata
     3601 gacattgttt ttgaaggcag gtatattttc ttctttgaca agagcaatcg cacagccacc
     3661 aaatccagct cctgtcattc gtgcacctaa gacaccttct tgtttttgag cagcatctac
     3721 tagtgtatct aattcgatcc cagttacttc ataatcatag cgaagagagt ggtgagaatc
     3781 atttaacagt tgaccaaatg cttctaagtt cccagcttct aattcagctt ttgccttcaa
     3841 agtccgctga ttttcagaaa cggcatggcg agctctgcgt atcaatgtat catcgccgat
     3901 ttggtcaata gttgattcaa attcttcttc atctaagtct cctaaagaag agataggcaa
     3961 agttttttgc aaacgtgcca aggcttcttc gcattcgcta cggcgttcat tgtattttga
     4021 atctgccaat tcccgccgct tattcgtatt catgatgacg attgcataac catctagttt
     4081 cactggaacc atttcgtatt tcagcgtatt tgtatctaat aagattgcat gatctagctc
     4141 accgaagccg atggcaaact gatccatgat acctgaattg acaccgatga attcattttc
     4201 tactttttta cctgtttgga ctagttccaa gcgatctgtt tttaaatcaa acagatcttc
     4261 taatacaaca cctactaata attctaatga agcgctactg gaaagtcctg caccattagg
     4321 gattgttcct tccactaata actcaaaccc atgatcgatt gagtgtccag cttcttttaa
     4381 tttaaggacc attcctttta cgtaatttgc ccagtttgct tttttatcat attctagctc
     4441 atctaaagta aaactaatga caccgatatc gtcaaagttt gtggaataaa ctaatatttt
     4501 attatcgtct cgaggtgctg ctacaccttg cgttccataa gtgatggaag cggggaagac
     4561 atggcctcca ttgtaatctg tgtgttcacc aatcaaattg atgcgtcctg gtgagaagta
     4621 ttgtgtagtt tttttaggtc cgaatatttc ggcaaatttt gtgtttaagc ttcttttgat
     4681 ttccatgatg agttattcct ttcttgtttt gattttgaat tctgtcactg tttcaaatac
     4741 tgatccggct tttaaatgaa tcgaaccaaa tgctgagaag cgctctgcac ctggagctgt
     4801 ttgagtctca aatgtgatgc caccgtgatg tgccagtttt cggttgcgca tttctggtgt
     4861 ttctgtgccg aaatttgcag tgaaaataac cacactagaa gcatctgtag caatatctac
     4921 ttggattctt ttatcggggc tagtcaagcg agcggcttct ttacccaagc cggtttcttt
     4981 taagaaaaat ggatgatcga ttccgtcaac aagatttttt tgatccaaat cactagcaaa
     5041 aacggacgct aattttttag gtatttgaaa atcgaaagca cttcctgcag cattttcttt
     5101 tacccctata ggaatactat ctgttcgcaa aggagcataa gcttcgctat tgagccaaag
     5161 ctcatgttga tcgattgact gagaagcatc tcccgtcaag ttgaagtaaa catgatttgt
     5221 cggattgaat aaagtatctt ggtcactggt gccgcgagag gtcactcgcc agatattatc
     5281 ttttgtcaaa gtatagcgga tttccacatc cattgtacct ggaaaaccat gttctccgtc
     5341 gggacttgta gtagtaaaaa tgacagaagc ttcattttcg ccattcaaaa tgacataaga
     5401 ccattttttt aattcaaatc caggagcacc accatgcaga ttatgtccag tttttggatc
     5461 tgtggctaat tggtaagttt ttccattgag agaaaagcgg ccattttcga ttctccctgc
     5521 ggtgcgaccg atagaagcac cgatatacgg atctttttcg atatattctt cagcggtgtc
     5581 aaatcccaaa actaattctc gatcatttga ttttaaactg acgattctag cacctaaatc
     5641 agagatagaa agtgctagct tgtttttgtt ctccaaggtt atcaagtgat aaccttggcc
     5701 aaaatctttt tctgtaatct tcattttcaa gatcctttct taatgggaag gaactactgc
     5761 atcgtgaagt taatcttcca ttgtatcagc tggagccaat ttgttttcca atttctcaac
     5821 gatttgtgca tgttttttct ctgtcaatgt aatcttccaa gcaaaaataa tagcggagaa
     5881 aatcatcaaa gcagcaggag catagaatgc ataagctttg aaagtcgtta cgccatgagc
     5941 agtgatatca gcagcagtag cattaccagt catcccagaa gcgatagcga caaaccctac
     6001 gatcccgtta gacaaagcac cagcgatttt atctagtaaa gggcgaattg atagagtaac
     6061 agcttcgtta cggataccat ttttccattg accatattca acagagtctg tgatcgtcat
     6121 caaagcggat aagaaaatca attgttgagg gaagtagaac aatactagcc cgatgataac
     6181 gattggcaaa cttgaacctg cgatcgtaaa gaaaaagtaa gaagaaatca tcattgcgat
     6241 ccctccaagg taaacgtagc gacgagagat gacgcgtgta agcattggga atagtggaac
     6301 agctactaaa ccaagtaagg cagcaactag accaacgatc caaaattcag ttggttggcc
     6361 aagtacatat ttgaagaagt agaataatac ggcagttgtt gctgcattgg caatcgcata
     6421 agcgatataa gcaagggcaa gccacatcaa ctgatcattt tgagcaactg ctttgaacac
     6481 atctttgatc ccagcttttt cattttgctg acggatcgca gtatcgactt cttttgtgcc
     6541 caaggcagtg ataatcgcag taccgcctga aataacagca gcaatcacac cgaaccagaa
     6601 ccagcctgaa gcgccttcaa tatgtttatg agtaacaaga taggtaaaga aagtaacgat
     6661 agggacaacg actagagtag taccatttgc acctagagaa gagccgaaac gagcaaacgt
     6721 agcaaatttt tcacgttcct ttgtttccgt actcaaagct ggaatcattg accaaaaagc
     6781 gatatcctta aatgaataaa aactatccaa taagatgaaa acgattgcaa ataatatgat
     6841 aaataaagtc gtattatgag tagctaagcc aaagaaattg gtaaagattg ctaccaagag
     6901 tacggaacta acaacgccgc cgataactaa ccatggtttg aatttaccgt attttgtacg
     6961 agtattatcg atgatgccac caatcaaagg atcaaaaata atttcaacta agcggatacc
     7021 tacaactaag cttgtaactg tagcaatcat ttttgcattc gttgccttat ctgttcctgc
     7081 gaacatctgg cttgtcacga ataccataaa ataagtgctt aatgtaacat agaatgtgtc
     7141 gtgtccaaaa gcgcctaaag cgtaggacac acgttggatc attttgcttt tcataatctg
     7201 tattcctctc ctgttcaata tataatacta agtaaaccgg ttacaacgaa taacaaagat
     7261 cttttaatga cttaaactta acaagaaatc gcttcctttt taatatattt ctgtgacttt
     7321 tatttaactt tttgttgcat tatactaaag gagtaaggag ggatagacgt ggagcaatcg
     7381 atttttttaa aaaaggaaca tcaacaagta gaaaaagaat tgtattttga tttttgtggt
     7441 ttctctaaaa cgatgccgtt tcattctttt ggtcctgcta ttcgcaatga ttacatcctg
     7501 catgtcgtga tggaaggcaa aggatcttat catgtgaaaa atcaacaatt ccaattgaaa
     7561 aaaggtgatc tgttcttgat tcgtccagga gactcgacgt tttacttatc agatggagaa
     7621 gatccgtgga tgtattgctg gctttcattt ggtgggagtg ctgcagaaga aatcattcgg
     7681 cagtcgcttt tcagagaaga ccaatacacg atggtctctt ctgacataca aaaatatatc
     7741 gacattattt tagaatgcat gaaagatacg gaagaaacct tggaaaatga attacagtta
     7801 acagcattaa cctaccgttt tttatcagtc cttttaaaag atggcggaaa ggtcagcgga
     7861 ggtgtacaaa aggttcattc ctctttagct gttgaaacag tcaaatacat cgaggcacat
     7921 tatgctgaaa aattgtctgt tgaggaaatc gcccagcatt tagcggtcaa tcggagccat
     7981 ttgtcgcgag tattcaaaaa tcatatgggc accagtatca aggaatattt gatcggggta
     8041 cggatcaatc gaggggcgtt tcttctttct ctgacggatg aatccgtgga aagtattgcc
     8101 tatcaagtag ggttcaatag tctagtcgta tttagccgaa tgttcaaaaa gagtaccgga
     8161 gaaacggcta cgaactatcg taaacgtatg aaaaacgaaa attttaagaa tattgctttg
     8221 gataccttga aaaaacagtt ggaaaaacaa accgcagttt cttggacaac ctgattagtc
     8281 tggttaaaat gacaagcatc ggcaaaagaa acttgtggcg gttatgctac actgtaaagc
     8341 gaagactaaa aaggaggaaa agggatgcgt acacttatca tcattagcca tccaacgatc
     8401 gaagaatctt taaatcagca atttttcaaa gaagcttctg ctggtttacc agctacttgg
     8461 catcatttgg aatcaagtta tccagacgga aaaatagata tagagactga actacagctc
     8521 ttggcccagc atgatcggat cattttccag tttccgtttt actggtatag ttctcctgct
     8581 catttaaaaa tttggcaaga taatgtcttg gaacgagcag cacatgtatt aaaagggaaa
     8641 gaactaggaa tcgtattgac aacaggtgtg gcggaaaaag aataccaagc tggaggaaaa
     8701 gaagaatata cgatttccga atttttgagg ccgtatcagc gaatcgcaaa taaattccat
     8761 atgacctatc ttccgccatt cgttttagca caatttatgt atttatccca ggaaaaacgt
     8821 tgggagaagc taattgctta tcagcagtat ctctcattgg aaggcaaacc atcgctgact
     8881 cagcggattg actggtttat tcaaagagta caggaaaatc agaaaatgca ggaagaagaa
     8941 tctgaaaaac aagcatatat cgtagaagca ctgactgagg caaaagagca gatcgaagac
     9001 ttgaccttta cactgcaaga aatgaaagga acttcattat gacaaatgat tggctgatcc
     9061 aacaattaga tgaattaagc gaaaaatcag aatatcaaga ccgtgcgctc ttatatgaaa
     9121 taaagaaact gatggctgag atagacgaac gaacaatgca gctgcaagga gaaatcgacg
     9181 gacaatcttg gaatcatctc aactggtgaa attttcttat aaataacaaa gataaaaatg
     9241 tgcacagcga caattatttg ctacaataag aatatgaagc gtttgataac gctattatac
     9301 ataaaaattg gtggtgaaaa aaatgtatga tgaaaatgag tggggagaaa atgttcggat
     9361 gacccgaaga agacagcaaa aacaggcagc tcgtaagcgg atgatcatga ttggggcagc
     9421 agttgccgtg tttttgatca tagcaggagg agttgcgggt ctatattttc atggaaaaaa
     9481 ttcgtcagaa gcgaaaagta cgaccataac ggaaacaacg acaaagaaaa cagagacgaa
     9541 acaaacaagt gagtcatcaa ctaaaacaaa agagacacaa acttctgaag aaaaaacagc
     9601 aaatggtttg ccagagcaac ttcaagcaat cgtaaatgat tattcgaaaa agatcgaaga
     9661 aaagacgcct gcattgattg aagaatacca gacagaaatt cagggaaatc aagagggaat
     9721 agctggttta tcagcagttg ctaaccaaaa agcacgtgag cttcaagcaa tctccgacga
     9781 agggatcaga aaattacgtg cagcgtatca ggcagcagaa aataaagacg gtgtagactt
     9841 agatactttg atcaatcaat tatcggctaa ttatacgaat catgtcgcta agatttccga
     9901 tatttatttg cagactagcg cctctcttca agcagagtca acaagcacac aagacacaac
     9961 atcttctgat tctgagacag cagagtcggt agaagcttct gaaatgcaag ctcgaacaac
    10021 acaagacaca acagattcct ctgaaactaa taccaacagc gcgacttcac aggctgcgac
    10081 aaccgtagtt ttggaaggag aaggtccaaa tcaaatagcc gaacgaactg gcgtaccagt
    10141 agaaacgatc ttgtcgctga atgggatgag catggagaat tatttcctga ctcccggtga
    10201 gacgctaaaa ttgaattgat aaaagcaata aaaaagaacg aagcttccac taaggtgaag
    10261 cttcgttctt tttttatttt ccggttcttt tttcgcgtac aaagtaatgg atcgtcatgt
    10321 aaagcaagaa aacgagtcca ataatcaatg agattctcgt ttcaggattg ataaacatga
    10381 aaatcaatac gatagctaaa aaagcaagtg tcacataatt cgtataagga gatagtggca
    10441 ttttaaaagg atgatcatgc attttatctg cgtgcttctt tttgaagcga atctcgctga
    10501 tcaaaatcac tacccaagga accataccag gcaaaatact tgagctgtat acgaaaacga
    10561 aaatattttc tgactggttg atgaacattg gtagaagata atttaagatc agaccaatta
    10621 agattccacc tgagattgct acgacaggat aaaaaggcgc accgtgacga gatagtttca
    10681 gaaatttttt agacatcttt ccatttatgg caagtgtata gaccatacga cttgcgctga
    10741 aaatccctga attgcaacct gataatgccg ctgtaatcac gacaaaatta atcagtcctg
    10801 cagcaaatgt aatgccgact ttagaaaaag tttgtacaaa aggtgaacca atctcactca
    10861 attgattcca cggatagata ctcacaatga taaaaatagc accaatataa aaaatcaaga
    10921 ttcgaccgat agtggactgg acagcttcaa tgatcgtctt tttaggattc tctgcttcac
    10981 cagcagtcat tccgatcagt tcgattcctt gataggaagc aacaacgatc gataatgcaa
    11041 agaaaaatcc tttgacgcct ccagtgaaga atccaccatt tttccaaaga tttgaaatcc
    11101 ctactgcctg accatgatta ccaaatccaa ataagattac cccaagacct gcaatgatca
    11161 tcaaaatgat cgttatgact ttgatgatcg aaaaccaaaa ttctaattcg ccaaacgcct
    11221 tgacagaaat aagatttgcc agcataagaa acaagacgac aatgattccg ggaataattg
    11281 ccggcaaatc aggccaccaa taattcatat aagatcctac agcaatcact tcacttattc
    11341 ctacgacaat atattgaaaa atattactcc aagcggtcaa gtagccagcc acaggatgga
    11401 tgtactcgct cgcgtaattt gcaaaagaac cagtcgaagg atcaacgtaa agcatttcgc
    11461 ctaaagcacg cataatcaaa tacaaaaaga atcctgcaat cgcataggct cccatcactg
    11521 agggccctgt ccaacgtatg gttgaggagg cacccataaa cagaccgaca ccgatcgttc
    11581 cacctagtgc gatcatttgg atatgacggg cactaagact tcgagttaac ttttcttctt
    11641 ccatacaaaa actcctttcg ttgttgagtc aacaagaaaa aataatcaaa cgtttcaaca
    11701 catgtttttc attattaggt tagccttatt gaactcttga tgtgattcta cagaaaattt
    11761 tcataaaatg cgaggaaaat tcgaaaaaaa ctttttcatc attctacttg aaaatgcttt
    11821 ccattttagt taaactatat ttaaataaaa agttaggtaa acctaaaaag gagtgtaaga
    11881 tttggaacgt acggccattg taattcaaga cctttcagcc gcttatcaag ggaaaactgt
    11941 tttgaaaact atcaacttga cgataaatcc acaaaaaatt accggtatta tcggaccaaa
    12001 cggtgcagga aaatcaacat ttatgaaatc tttgctagag ttgataccag ctactactgg
    12061 taaagtcacc ttttctggac agccggtaaa ctcagtccgt aaaaaaatag cctacgtgga
    12121 acagcgaagt gaattggatc tttcctttcc aattgatgta ttaggcgttg tacttttagg
    12181 aacatatcca tctttacgaa ttggacaaag acctgggaaa aaagaaaaag aacgtgcaag
    12241 acaagctttg aaaaaagtag ggatggaaga atatgccaaa agacagatca gcgaactatc
    12301 gggtggacag cttcagagag tttttattgc aagagctcta gcccaaggag cagaatggat
    12361 ctttttagat gaaccattcg tagggattga tgcgttaagt gaacgaaaga tctttgacat
    12421 cttgcaggaa ttgaagaatt caggaaaaac gattttgatc gtccatcatg atcttcataa
    12481 agtagaagaa tatttcgatg aggttattct tttaaataaa cagctgatcg cttccggtcc
    12541 agtacaagaa gtctttacat cgaagaacct tcaattggcc tatggtgaga tcatcagaca
    12601 tttagtaaaa ggaggagaga aaaaatgatc caatccttta ttgatggatt aatgaattat
    12661 caattcctgc aatacgcttt ggtcacttcc atgcttgtag gacttgcttc tggagtcatc
    12721 ggttctttta ttattttacg agggatgtct ttgatgggag acgctatctc tcacgcagtt
    12781 cttccaggtg tagccatttc ttatatgttt ggttttagtt acatctttgg agccactgct
    12841 tttgggatgc taacagcagc agtgattggt ttcgtgactc agcggagccg attgaaaaat
    12901 gatacagcga ttggaattgt ctttagctcc ttttttgcat tagggattat cttgatttca
    12961 tatgcccaga gtgcgacaga tttgtatcat attttatttg ggaatgtctt agctgttagg
    13021 gaaagcgatt tgttgctgac agcactcgtt agtggaatcg tcttgatatt tgtctttttc
    13081 ttttataaag aattgaagat cacatcattc gatccgacaa tggcaaaagc ttatgggctg
    13141 aacacatcgc tgatccatta tcttttgatg ttctttttga cattagtggc tgtagtcagt
    13201 ttacagacag taggaacaat cttggtgatt gccatgttga tcacaccagc cgccacggct
    13261 tacttgctaa cgaaccattt actgaaaatg atcattacag ctgcaggaat cggtatgcta
    13321 agtgcagttg tcggtgtgtt tttcagttat agttacaatt ggccatcagg agctacgatc
    13381 gtgttagcat gtaccgcatt ttttatcctt gcttttttat tttctccaac aaaaggaatt
    13441 ttatttcaga aaggaagcag atagatgaaa acaaaaaaat cactcttttt aatcttagca
    13501 gttagtttct tggtattagc cggttgcgga aagcaggctt ctgaccaagc ggatgaagga
    13561 agtaaagaaa agttgtcagt cgtggctacc aattcgatct tggcggacat ggcaaaagag
    13621 gtcggaacag atcaaataga tatccacagt atcgtaccag tcggaacaga tccgcatgaa
    13681 tatgaagtac taccagaaga catcaaaaag gcaagcgatg cagatgttat tttatacaac
    13741 ggtttgaatc ttgaaacagg taacagctgg ttcgataact tgatggaaac ggctaaaaaa
    13801 gaagaaggaa aagattattt tgcagttagc aaaaatgtag aacctcttta tttaactagc
    13861 ggtgaagaac atacaaaagc agatccccat gcttggctag acctatctaa cggaataaaa
    13921 tatgtggagg aaatcgcacg tatattctct gaaaaagatg cagaaaatgc gacactctat
    13981 aaaaaaaatg cagaagcata tgtggaaaaa ctaaaagaat tagatacaca agcgaaggaa
    14041 agttttgctt ctatcgaaga gaacaaaaaa ttattagtaa caagtgaagg ggcattcaag
    14101 tatttttcac gagcatatga tttgccagca gcttatatat gggagatcaa tacagaaagt
    14161 caaggtacgc ctgatcaaat gaaagcgatc attgatcaga taagagcaaa agaagtaccg
    14221 gttttattcg tggaaaccag tgtagactca agaagcatgg aacgggtagc aaaagaaacg
    14281 ggtttgaaaa tctatgataa actgttcact gattccatag caaaagaagg agaacaagga
    14341 gattcttact atcagatgat gaaatggaat attgaaacga tccatgaagg tttgtcacaa
    14401 acaaaagaaa gctgaaaagt gcaaggtcta gcttgaggag cagaccttgc attttttata
    14461 agagatatga aaaaaaatta tgctgttttt cgtcgtacag ataccttttt tgaagaaatt
    14521 ccgttatagt tagagtaagg aaggaaggtg tggctatgaa ttttacacgt aaagataaat
    14581 atacgattaa agaagaagtc tttcgattga tcagccagca ggaaaatacc gacaaggatc
    14641 aagaccttca atttcttgaa gcagaattag ctaaattatt catagcaaat caaaatgaga
    14701 caacagaacc agatgaagag ctgtacatag gcaaatggtt gtttgatgaa tttcagcctt
    14761 tagatgaaaa taccggtatc ggttattttg tgttcctttc tccagtcggc ttcacacctg
    14821 gcgtattagt taaacaaaac gacgaaataa ccgtcaaagc atggcctgca ttattttcag
    14881 tcatcaaagc agaaggagta atcaaaaaga tccagtcggc ctattataaa agtcaattta
    14941 ttgccaacag caaattaatg aatatgtttt ataatcgtct agtagaaatt gctaggcctg
    15001 ggaaaatccc gcaaacattc acgtacacac agcaaaaaaa tctctatgaa gaagtattgt
    15061 attcccgcga tctgtctgag ttttcgccat gggaagaggt aaggctccat gaactgttaa
    15121 gtacaaaacc agtttcattg aattttttta atgcattaga taacaaaaga aaattaattt
    15181 caacaaatta ttgggaagta tttgctgcaa gaaaaaaatg gatggaagaa aagaagattg
    15241 ccgatcagca gatccagttt gtccagctga aaaaagaaaa actatattat gggttataca
    15301 gccgttaaca gcaagcgagg aagaccatga cattaaaaga agaaatcata caagaaagca
    15361 aacgattagg aattgacaaa atcgggttca ccacggcgga accttttgat tcattaaaag
    15421 aatcgttgga agaacaaaaa gcagctggcc atacttctgg ctttgaacat ccaaatatcg
    15481 atgagcgtct ttatccagaa atgacatttg atcagccgaa atcgatcatt tccattgcac
    15541 ttgcgtatcc tactaggatc catgaagaag tgcctaggga tgaaaaacgc gggcagtttg
    15601 ctcgtgcttc ttgggggatc gattaccatg atatactcag agaccgtttg aaccgattga
    15661 tcgaattcat ccaaacaaga gcagaaaaat ggcaaaaaga agaggaatgg cgtctagctc
    15721 cgcaagtaga tactggagaa ctgatcgatg tcgctgtagc acagcgagct ggtttaggat
    15781 ttattggacg aaacggattg ttgatcactg aagagttcgg ttcatttgtc tatcttggtg
    15841 agatcgttac taatattgta tttgagcctg ataaaccagg agaatttggt tgcggtgact
    15901 gtatgcgctg cgtgactgct tgtccgacaa aagcattgct tggtgacgga agaatgaatg
    15961 cccagcgatg tttgtcttat caaactcaga caaaagggat gatgccggaa gaatatcgta
    16021 aaaaaatgcg aaatgtcatt tatggatgtg atatctgcca gttagtctgc ccttacaatc
    16081 aaggaaaaga ttttcatttt catgaagaga tggaaccaaa agtagacgaa atatatccaa
    16141 aattaaagcc aatgctttcg atgtctaata aagacttcaa acagcaattt gggcatttag
    16201 ctggttcatg gcgtgggaag aaacctttgc agagaaacgc attgattgct ttagctaatc
    16261 taggtgatcg aacggcattg cctgagattt cagaatgttt aacagatgtc cgccctgtca
    16321 tcagaggaac agcagcttgg gctattggac gtctaggaac aaaagaaccg gaaaaatggc
    16381 ttgatgtatt agatgaaata ttagctaaag aaacagaaga agtcgttata aacgaattgg
    16441 aacatgccca gaaattattg cggagaaagt taaaataaaa aaatagttaa cattattcaa
    16501 agtttttgtc cagtaattgt tgtaacaatt actggttttt ttgtattttt tacatttttt
    16561 taagagaatt tgataaaaat caaaaatttt tttgcttttt tttatggatt attgcagtga
    16621 atgttacaga atctgcgttt ttcattcaaa aataaaaaaa catagaaaaa cgattacttt
    16681 gct
//
LOCUS       DAGCYL010000046        16588 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.047, whole genome shotgun sequence.
ACCESSION   DAGCYL010000046 DAGCYL010000000
VERSION     DAGCYL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.047"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1474)
                     /locus_tag="MB216_001165"
     CDS             complement(<1..1474)
                     /locus_tag="MB216_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4526013.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRI"
     gene            complement(1471..2157)
                     /locus_tag="MB216_001166"
     CDS             complement(1471..2157)
                     /locus_tag="MB216_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4526014.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2350..3771)
                     /gene="gndA"
                     /locus_tag="MB216_001167"
     CDS             complement(2350..3771)
                     /gene="gndA"
                     /locus_tag="MB216_001167"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBT4526015.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(3941..4120)
                     /gene="rpmF"
                     /locus_tag="MB216_001168"
     CDS             complement(3941..4120)
                     /gene="rpmF"
                     /locus_tag="MB216_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBT4526016.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4178..4747)
                     /locus_tag="MB216_001169"
     CDS             complement(4178..4747)
                     /locus_tag="MB216_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBT4526017.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            4933..5835
                     /locus_tag="MB216_001170"
     CDS             4933..5835
                     /locus_tag="MB216_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4526018.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(5870..7654)
                     /gene="pyk"
                     /locus_tag="MB216_001171"
     CDS             complement(5870..7654)
                     /gene="pyk"
                     /locus_tag="MB216_001171"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBT4526019.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(7728..8690)
                     /gene="pfkA"
                     /locus_tag="MB216_001172"
     CDS             complement(7728..8690)
                     /gene="pfkA"
                     /locus_tag="MB216_001172"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBT4526020.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(8858..12172)
                     /gene="dnaE"
                     /locus_tag="MB216_001173"
     CDS             complement(8858..12172)
                     /gene="dnaE"
                     /locus_tag="MB216_001173"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBT4526021.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLIKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLIENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKDGSQIPLENLSASLPLT
                     ANGYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITNYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLGPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            12399..12584
                     /locus_tag="MB216_001174"
     CDS             12399..12584
                     /locus_tag="MB216_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBT4526022.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12646..13842)
                     /locus_tag="MB216_001175"
     CDS             complement(12646..13842)
                     /locus_tag="MB216_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBT4526023.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSESTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFILSNIFLSLFNFRK
                     VLRSRL"
     gene            complement(14012..15448)
                     /locus_tag="MB216_001176"
     CDS             complement(14012..15448)
                     /locus_tag="MB216_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBT4526024.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNEVSVALWIVDILFIINFIILATI"
     gene            15741..16550
                     /locus_tag="MB216_001177"
     CDS             15741..16550
                     /locus_tag="MB216_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4526025.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4864 a         3500 c         2915 g         5309 t
ORIGIN      
        1 agattctaaa aattgtccct tgccctacta cactttctgc taagatacgt cctttatagt
       61 tttcaaccag ttgtttagcg atagacaacc ccagaccatt tccacccttc gttctagctc
      121 ttgctttatc cactcgataa aaacgattga agatcttatt aaggtcttct ttgggtattc
      181 cttcgccaaa gtcctgaatc gcgatctcaa attcattcag agtggaagaa atagagatat
      241 ggacttcttt tcgtgtagtt gaatacttta ctgcgttatc caatatgata atgattaatt
      301 gttcaaagtg attacgataa atttgcaggg tcacttcttt tgtcaaatca tcatctaaag
      361 tgatcacata gtctggatat aatatcttga aattattata aacttgatag gtgacttctt
      421 ttcctttagt tgtttcattc gcataatgaa catctacttg ttctgctctt gataagtcta
      481 gcatctcttg aactaagctt ttcatacggc taatttcttg catgctggcg cttaatgatt
      541 cttctaatat ctcaggatca tctttccccc aacgatttag cagactaaga tgcccttcaa
      601 taattgcaac aggtgtcctt aactcatgag atacgtcttc cacgaattgc tcttgttgtt
      661 caatatacaa acgcatacgg tcaagcatct cattgaatat ttcagctaaa tctgccaatt
      721 catcatttgt gtcgatttca ggcatgtgga tatctgattg cggatcttta cgtatagtat
      781 ccattgtatc tctgagtacc ttgagtggtt ttaagaaata agaagataaa aagaaaccta
      841 aaatactact taagatcaaa gaaacaactt ctaaaacaat aagagtcaat aatagcttat
      901 ttcgaatatc atagaaagaa gataattcat agaagctttg cgcatagccg atcttttctc
      961 ttgtaccttt agaatatata ggctggatag ataaaaagcc agtttttcct tctagtgtaa
     1021 cgatcgttgg aactttacgt gtagtctgaa tcaggtttcg actgttttca tgggttttaa
     1081 aaatcaagtt ctcgtccaaa tcataaacat aaagatccat tgatggctgc cctagctcag
     1141 ctataaagga atcgatcttc attaagtttc cttcgaccag tgaagtatga tctacgacct
     1201 catctccgtc atttgtcgct ttcgtcagat tacgatacgt atttactaat gtcagttctt
     1261 catccgagtt agccaaacga gaagctactt ctgaaatcgt acgctctaca ttgtttcttt
     1321 cttttgccac aatcagattg atagatgatt tataggtaat gacagcgaaa attgtaaaca
     1381 caacgaatat gaagaaagaa ctcgcaaagg cccacttgat tgtcaaggag ggccctttca
     1441 gttctctttt ttccgatttt ttcggcttta tcacgagcgc atcacataac ctgttccgcg
     1501 tacagtttgg atatagcttt cctccccggg aacgtcaatt ttgttgcgaa gataacgaat
     1561 atatacgtcc actacgtttg tttcaacttc tgtttcgtat ccccacactt tatttagtaa
     1621 aacgtcacgt gctaatacca cgttcacgtt ttccattaaa gtaagcagca actcgtattc
     1681 tctttttgtt agttcaatta cttcatttcc gcgacgtacg acacggtttt ctttttcgat
     1741 agtcaaatcg cgataagtaa tagtagtttg tttagccaca tttttatcgc cttcaatatc
     1801 gattcgacgc aacaatgcac gaagacgtgc taataattct tcgatagcaa atggtttgac
     1861 gatgtagtca tctgctccgt ggtcaagtcc agatacacga tcgatcacag aatctcttgc
     1921 agtcatcata atgattggtg tatttttgac ttggcgtaca cgtctgcaga cttctaaacc
     1981 gttcaattct gggagcatca gatccaacaa tatggcatcc cactcattat ttaatgccgc
     2041 atctaaacca gttcggccgt tgtagtggac ttctgcgtta tacccttcat gcttcaactc
     2101 tagctcgaca aatcgtgcca agttcttttc atcttcaatg attagaatat tgctcattta
     2161 ttattttatc ctttctattc tactggcttt ttaaaaactc tatcattata ccgataatat
     2221 gcttaaaaat ctatagtcat tttatcatgg atagcaattt taaaaaagag ctgaagtcta
     2281 ctcataacaa tcgacttcag ctctttcgtc ctctttttat tttctcattt cctaacagcc
     2341 gaaagactat tattcttcat ggtaccatga gtaatggaag attccttctt tgtcgacacg
     2401 ttcataggta tgtgcgccaa agtaatcgcg ttgggcttgg attatatttg caggcagacg
     2461 ttctgctctg tatgaatcga agtaagcgat ggctgatgag aaagtcggta caggtacgcc
     2521 agcttggaca gccaaggcaa cgacgtctct gactgattgc tgatattttt tcgtgatttc
     2581 aacgaagtaa tcatctagca ataaattttc gatctcagga tttttgtcat aggcatccgt
     2641 aattttttgc aagaatcgag ctcggatgat acatccagcg cgccagattt ttgcgatttc
     2701 accaaatggc aagtcccaat caaattcttt tgaagcgaca cgtaattgag caaatccttg
     2761 ggcataactc atgattttac tgaaatacaa cgcttctcgg attttttcga ccaattcttt
     2821 tttgtctccc gtgaactcaa aatcgttcgt acgagataaa atcttgcttg cttgtacgcg
     2881 ttcttcttta taagcagaaa tatagcgggc aaatacagat tcagtgatca atggaagtgg
     2941 cacacctaag tcaagcgcgc tttggcttgt ccatttacct gttcctttat tcccagctgc
     3001 atcaaggatc acatctacta ctggtttacc tgttccttca tcgtctttac gtgtcagaat
     3061 gtcagcagtg atttctatca aatagctgtc taattcgcct tgattccatt ctttaaagat
     3121 atcagccatt tcttctactg aaaggtttaa aatattcttc attaagtcat aagattcagc
     3181 aatcagttgc atatctccat actcaatacc gttgtgtacc attttcacat aatgtccggc
     3241 tccatttggg ccaatatatg tgacacatgg tgcttcgtct tctgcttttg cagaaatttg
     3301 ctctaagatt ggtgcaacta gcgcataggc ttctttttgt ccaccaggca tgattgaagg
     3361 tccttttaaa gctccttctt caccaccgga aacgcctgta ccgataaagt tgatgccaga
     3421 gttggctaac tcttcattac gacgcatcgt atctttaaag aatgtatttc ctccgtcaat
     3481 caaaatatcg cctttatcaa ggtgaggaag caactcttgg atcgttgcat ctgttgccgg
     3541 tcctgctttg accattagca agatccgacg tggtttctcg atagattcta caaattcttc
     3601 gattgtatat gttgctttga aatttttatc tgggtgttct tctactacag cggttgtttt
     3661 agcccctgta cgattgaata aagccactga atagccgcgg ctttctatgt tcaaggctag
     3721 atttttaccc ataacggcca taccgacaac gccgatttgt tgttttgaca tatgaactcc
     3781 tcctacatac gggatttctc cctaatctct tacattatag cttaacacgg ctacattgca
     3841 aactaaagaa caacatattt tttatttacc gactacaaaa aaataaacct caaggctcca
     3901 tggttagcct tgaggtttta cattttctaa ttaaaatgta ttaagcttct tttgacatta
     3961 cgtccttacc atcatagtaa ccacaatttg cacatacgtg atggcttttt ttcatttcac
     4021 cacagtttgg acattcgttt aaccctttga ttgtcaattt gtagtgtgta cgacgttttg
     4081 cttttttagc ttttgatgtt cttctagctg gtactgccat tttgctacac ctccttgtaa
     4141 agtcttgtat ctaaattaga cacaatatat tccaatctta cttgttatcg tcgtcttttg
     4201 atttagaatc aaaaagttca gatagttttg caagacgagg atcaatttta ttctcggctt
     4261 cttccatttt tctgcgctgg tattcttctt cggaaacaac ttcccaatca ttgccttttg
     4321 gaagttctgt tgctgcttcc tcttcttttg tcaatacccg cattggaatc gccaataaga
     4381 tgttgtcttc aatcgactct gctaaattca ataatggacc ttcaattagc aaaatctcat
     4441 ccgcagacat ttgttcatct ctactttgga attgttcttc tgtcatgaat acttcgtcta
     4501 cagaaaaatc catgggcagt tcaacaggtt ctagtgaacg tgttgaagga actgtcaaag
     4561 tcgtctgcac agtgtaatga acgatatagc ctttcttatc aactgagacc aaaccttcga
     4621 cttccactgg cgcaacatca agtattaaat tgtctcgctt catgagagcg gctttcaagt
     4681 cgagcgtctc atgaaaagtt aacggtgtct cctggtattt tctcaattcc aataaagacc
     4741 atttcatttt tcatcactcc taagcaacaa aagcaattat acagggcaaa aagcatgttg
     4801 tcaatgaaat tttcttgaca ccttgcagtt cataaaaaaa cagaaaaatc tttattagaa
     4861 agcctctgtt tgctatcatc ctcagaatca tttacaataa tcattattcc attaaaggag
     4921 cggatgatct tcatgaacac gaaagatatc gaatactatg tccaaatcgt caaacaaaaa
     4981 aattttacga aagtggctaa ttatttccgt gtgagccaac ctactattac atatgcatta
     5041 caacgtcttg aaaaagagtt tgacaccgta cttattacaa gagatcgatc gcatcacgaa
     5101 ttaatcgtca cgccttctgg aaagcagctg tttctccatg cacaaaccat tctccaagag
     5161 atttctactg caagagctga aatcactcag ttgcaaaaga aaaagcttcg ttttggcatg
     5221 cctcctatta tcggcaatga atactttcct aaactgtcaa gttactttat caagcatgaa
     5281 ttgatgaatc aaattgagat taccgatgga ggctctcggg acttatacgg gatgatccga
     5341 caagggagga tcgatttagc tgtattaggc tcaacacagc caatcgtgga tcaatattta
     5401 gaaacagagt tacttttgga caaacgtttc atgatcgtcg tgccacctac acatcctctt
     5461 tccaagcagg atactgtctc tttcaaagat atagcagatg aacagtttgt actattgaac
     5521 gaacattatg tccaccccgc agcattcact aagctttcta aacaaacaca tactcatcca
     5581 aaagttgttt atcaaagcaa tgacttgacg attttaaaga gtatggtccg agaaaatgtg
     5641 ggtatcggtt ttttggcaga gattgctgtc catcctgaag atcatttagt ttgcctccct
     5701 ctttcagatg aagttcagcc tcgcttcttg atttcattgg caaaacgaac acagcagatc
     5761 gtcacgcctc ttcaagaaca agtagtccaa gttatccatg aattcgttac agcacaaaaa
     5821 aacagctctt cgtaaaagaa gagctgtttt tattttttat taagctaaat tagatagctg
     5881 ttgtttcgcc acggtaaacg atgccgcgac gtgaatcaac ggtaatcaat tcaccttctg
     5941 taattgttgt tgttgcgcct ttagcaccaa cgattacagg aatatcttgt gcaattccta
     6001 caactgcagc gtgagaagtc aatccgcctt cttcaacgat aacagcagct gctttttcga
     6061 ttgcaggcat atattctttg tcagttgatg gtacaactag aaccattccg tcttttgctt
     6121 tttttacagc ttcttcagca gatgaagcaa taactgcatt tgcaacgaca gatttttgtc
     6181 caacgccttg agcttctaat aatttagaac caatcatttg aattttcatc acgttagttg
     6241 ttccgcgttc accaactggt acaccagcag tgatcaagat taaatcgcct tctgatgcga
     6301 aacctaattc aacagctttt ttagctgcta gttcaaacat ttcatcagtt gttgatggtt
     6361 tttcagcaac tgttgggtaa acgccccagt ttaacatcaa accacgtttt gtgcgttcgt
     6421 caaaagtaac agccaagata tctgcatctg gacgatattt tgaaatcatt tttgcagtgt
     6481 atcctgattc agttgcagca acgattgttt tcacgcctag attttttgca gcacgagcta
     6541 cagataaacc gattgtttca gttacatctg ttttatcgaa ttcgttcaat tggaatgtac
     6601 ctaattctga taatgcattt tcagcttctt ggtcgatacg tgccattgtt gaaacagctt
     6661 caactgggta gtccccgttt gcagattcac cagatagcat tgttgcatct gtgccatcaa
     6721 atacagcgtt agcaacgtca gaagcttccg cacgtgttgg acgtgggttt tcttgcattg
     6781 attctaacat ttgtgtagca gtgataactg ggataccagc agcattacat tttttgatga
     6841 tgcgtttttg aaccattggt actagttcag ctgggatttc aacacccata tctccgcggg
     6901 caatcataac accgtcagaa actttgatga tttcgtcaat gttgtcgata ccttcttgag
     6961 attcgatttt agggaagatt tgaacatgag tcatgtcttt ttcttctaag atttcgcgga
     7021 tatctagaac gtcttgtgct ttacgtacaa aacttgcagc gataaagtcg atatcgttgt
     7081 ctaaaccaaa acgaatatcg tcagcatctt ttggagtaat acctggcaaa ctaatagaaa
     7141 cgcctggtgc atttacccct ttacgtgatc ctagcatacc tgcattttta acttctacga
     7201 ctaattcacg gtttgcttcg tctttttcaa tgatttgcat atcgatcaaa ccatcatcaa
     7261 ataaaatgtg tccgccaacg tgaacatcgt caaataatcc tgggtaagta accgcgattt
     7321 tttcttttga acctacatgt tcaggatcca tagagatgcg tgtttgatct cctacttcaa
     7381 atttgatgta tcctgcacga ccaaaatcag cttctgttgt gccttgaaca gttgtacgga
     7441 tttcagctcc ttttgtatct aaaagaatac cgacatcttt tcctgtcttt ttaactgctt
     7501 cacggaccat agtcatacgt gataattgtt cttcatggtc accatgagaa aagttaaagc
     7561 ggaagacatt tgccccagat tcaatcaatt gagaaataat ttctactgag ttacttgctg
     7621 gtcctaatgt actaacgatt ttggtttttt tcatttaaaa aactctccca ttcaattttg
     7681 tttatttaaa gctgtacaca gcttaaatag cttggtttct ttcactctta aaatgaaagt
     7741 gaattattta gatcatatag tgataagtct ggtttatgtt tgttgttttc aagtgtatca
     7801 atgatgtcag acgcaacgat ttcgttgtca tggataccga cacataatcc accttttcct
     7861 tcttgaagca attcgacagc gtaagcaccg aatttacttg ccaacacgcg gtcgcgtgcg
     7921 cttggtgctc caccacggac aacgtgtccc aagatagata cacgtgtatg atagtcgcca
     7981 aattctgaaa gtttctcagc gaattcattt ccgcccatta caccttcagc caagatgatc
     8041 aagcaatgtt tcttcccgcg atcgcggcct tcttggattt ttttagcaac tgaagccata
     8101 tcaaaatcat gttctgggat gatgatttca tccgctccgc cagccacacc tgaccataaa
     8161 gcgatgtctc ctgcattgcg tcccattact tcgataacga atgtacgaac atgagaagtc
     8221 gctgtgtcac gaatacggtc aattgattct agtactgtat tgattgctgt atcaaaaccg
     8281 attgtgaaat cagtcccagg aatatcatta tcgattgttc caggaatacc tactgctggg
     8341 aaaccgcgtt ttgtcaaagc cattgcgcca tgataagagc cgtctcctcc gataaccact
     8401 agaccttcga ttccgaattt tttcaattgt tcgattcctt ttaattgacc ttcttcagtt
     8461 gcaaattctg gataacgtgc agaatacaag aatgtaccac cacgttggat cttgtcacct
     8521 acatctgcca catcaagacg acgaatatct ccagcaacta aaccggcaaa accataattg
     8581 atcccgtata cttccagtcc ttcgtaaata cctttacgaa caacggcacg aacagctgca
     8641 ttcattccag gtgcgtctcc gccactggtc aaaattccga tgcgtttcat aataaacatt
     8701 cacctcatta aaatacttta tcctaagtat gacttaggtt tacaaccatt tttgcgttaa
     8761 ccacgactaa cattctaaca ttatttttct cacttgtctt gatttacgga taaaaaaatg
     8821 aaaaaaaatg aaagcaagta gtatttttta ccgtaagtta tttgaaaacc acattatctt
     8881 cgccaagcag ataaataagt tgccccttta tactcttgct gtcatccacc caataagcat
     8941 ctgacagaac tacttttcgt tgcgtttttt cgtgatacat aactactggt atatatcctg
     9001 ggtgtttctg aaaaatcact tgtaattgtt tcattatctc gggatcttcc cgttcttctg
     9061 taatacgcaa atagcaagtt tgtgaaggat actgggcttc taactggtta ggatcagcaa
     9121 agctttctcc aattagttgc agttcctgat tatatttgct gatttcgctc cgcccttgaa
     9181 caaagatcac tcgatcaacg gcaaacaaat tacgatattt acgataaaga gttggaaaaa
     9241 ctgtgacaga aatttctcca ctactgtcgg ttccttcaac aaaggccatc agttctcctt
     9301 tttttgtccg aatttctcta acttccttga tcatcactaa taaattgact ggttgattag
     9361 ggacgatatc tgtaatcagc tgaacttgtt tagctaaacg aagacgatca aacccttctg
     9421 caggatgacc tgacaaataa atacctaagt actcctcttc cagcttcaag cgttcttcta
     9481 gtgtatagtc agcgacttct tcttctttta atgccatgat tcccagcaag tcaagacttc
     9541 caccactgta aacgatattt tggatttttc cttcgagatc aagcatcaat tgtttcctgt
     9601 taggagataa ttcatcaaaa acgcctactg ctgtcagtga ttgcagtaac tctaatttca
     9661 gccaacgctt atcgatccgt atgaggaatt gatccactga ctggaacggg ccattttgtt
     9721 ttctttcgtt taatatatct tctacaaaat cacgccgaat accttttatc gctgtcagtc
     9781 cgaatctgat ttcttcctta ttgaccaact caaaactgga attactttga ttaatggacg
     9841 gcgaggaaag tttgagttta tttttacgtg cttctgcaat atattctttc aattttgatg
     9901 tgttatgccg aacagaattc atcaggctgg taaagaaagc acctggatga tgggctttta
     9961 gatacgccat ttggaatccc acgaatgaat aagcaaatgc atgtgaccga ttgaatccat
    10021 agtttgcaaa tttttcgata tattcatata cggtcgctgc tgttttttcc gaatatccct
    10081 gttgtaatgc tcctgataca aaatgcgact tttcttcatc taatacgtct tttttctttt
    10141 tactcactgc ccgtcgcaga atatctgact gccctaatgt aaatccagcc atagtcgagg
    10201 cgatctgcat gatttgttcc tgatagacga tcactccata tgtgtttttc aaaatcggga
    10261 tcagtgaatc atctgggtag cgaatggctt cttttccttt cttcctggca atgaaggtat
    10321 ctatattttg catagggcct ggtcgataca atgcattgac tgcggcaata tcttcgatgt
    10381 tttctggacc taatcttcgt aaaacgttcc ggataccagc agattcaaat tgaaatactc
    10441 ctgaggtttc gcctcgttga aacaagagca gcgtttttgc atcatttagc gggatctctt
    10501 ttaatcggat ctcataccct tctactcgct taacagcttt caaagtaaaa tcaataatcg
    10561 ataagttgcg tagtcctaaa aagtccattt tcaaaagacc gattgcttct acctcattca
    10621 tcgtatactg ggtcagaaaa gcttcatcag aaccgcgttg gagcggaact agttcaagta
    10681 gattcttatc gctgataact acacctgctg cgtgagtcga aacatgtctt ggcaatcctt
    10741 ctaaaatcac tgcagttttg aataaaagtt ggtttttttc gcttgcctga actaattcta
    10801 ctaattgatg cgaagtttta taagcttggg tcaatgtgat tttcaattga ttgggtattg
    10861 cttttgacca gcggtttgct tcactttgcg ataaaccaaa cactctagct gcatctctaa
    10921 gaaccatttt tgctgccatt gtgccaaatg ttgcgatttg tgccatatga tattgtccat
    10981 acttttctcg aacataattc aatacttctt cacgtcgatt gtcaggaata tccaaatcga
    11041 tatctggcat cgtaaagcgt tcggggttta aaaagcgttc gaacagcaaa tcatattcta
    11101 atggatccac atctgtgatt tctaacacat aggctactaa tgaacccgca gcagaaccac
    11161 gacctgcacc ggtaacgatt ttgtttctat gagcaaaagc cattacatcc catacgatca
    11221 agaaataatc atcgaatccc atttgatgga tgacagataa ctcgtagtcc aaacgttctt
    11281 ggtattcctt tgtataatta gtgattcggc gttccacttg cgaatagcat aactctctta
    11341 aatattctcc agcttgtttt ccattaggaa ctggataatg cggaagtaat ttctgatgga
    11401 actggaattc aaatgaaatc gattcagcta aacgctgggc gcctattaca gcttgggaaa
    11461 gacccgcttt ttcgaatcgt ttgatcattt cttgttctga aagaagatag ccatttgctg
    11521 ttagtggtaa agatgcagat aaattttcta aaggtatttg gcttccatct ttgatatgtt
    11581 tcatcacttc aagtgcaaaa agttcttctt gttgtaaact agcgatggga tgtaatgcac
    11641 aaagcgaata attttctttt atcacatact gataccattc aggcaattga tcaggcgtgt
    11701 tttctatcaa gccgatataa aaatttctag taaaaacatt ttcaagtaaa aactgccaat
    11761 gaccgaaaaa gttatcctct ttattgaaag cctcagaaac agaatgattt gccggaacaa
    11821 caaacgctag atggtcggtt tctgtcattt ctgcaagttc caatttttct tggatcattt
    11881 ttcggctgga aagttttatc agctgctgat aacctatttc atcttttgcg tacgcaagca
    11941 agccataatt tttttcttca taagtataac taagatgtaa tccaatgatt ggctgtactc
    12001 cttcttctac acaaatacga tagaattcag gtgcaccgta taagacatct ttatccgtta
    12061 tggctaatga agtgtagcct tgtttttttg cttcttgtac atattctcga atacgtatcg
    12121 tactttgtaa aagagaataa gaagtaattg tataaagttg tgggaaaaac atcttgcatt
    12181 ccgccttttt tttcgttaaa attcactagc gttaaaaggt ttgatccttt tactttcatt
    12241 aagatagcag gcgctgaagt tgattattcc acttcatttg cttgtaatct gtaggaatga
    12301 gttctttcgc aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga
    12361 aaatctggta aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat
    12421 tctgggctgt cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa
    12481 catatgattt tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga
    12541 ttggtgcagt tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc
    12601 ttgagatatc tcaagctctt ttttattttc tgctcctcac caccattata atctgctgcg
    12661 aagaacttta cgaaagttga ataaagaaag aaaaatattt gacagaataa acaaacttgt
    12721 cacaataaaa acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc
    12781 taaaacttgt ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc
    12841 ttgaggcgta atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa
    12901 gcctaaaata aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa
    12961 ataacagcaa aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc
    13021 ccctagtttt cctagtttcg gtgaagaaaa aacagcggaa acaccagcta tcgaaacaat
    13081 caagccgctg acaaaaagga tattttctgt actttcgctc aattcacgaa tatataaggt
    13141 caaaataggg ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc
    13201 gatcaaaata gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt
    13261 tttctcgatc ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat
    13321 caacaaagcc cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc
    13381 gcccattgaa ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc
    13441 ccatccgctc ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata
    13501 tccagacaaa attccattcc aaaaccgcat gatcaaaagc caatacgcgt tcggtacaaa
    13561 agccaaagat cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg
    13621 tccttttcga tcagctagat tcccccagat tggcgcaacc acggcagatg caaaagcagt
    13681 cacagagatt gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc
    13741 aacataaact ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc
    13801 aatccacgca atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt
    13861 ctcttttatc atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa
    13921 aatcccgcaa aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag
    13981 aacaaatact gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga
    14041 taatgaataa aatatcaacg atccacaaag caacagaaac ttcattgtat tttcctttga
    14101 ttactttcac aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc
    14161 acaatcccat aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt
    14221 tgatgtctgc aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg
    14281 cttgatttgg tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg
    14341 ccactactac tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga
    14401 cataagttgt tgtattcgat gtaccaaaga tagcccccac ggaagtagcg atcgcatcgg
    14461 caaatagtgc tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt
    14521 cttcttttga gaaaatgcct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga
    14581 aagtatcaga taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat
    14641 cactgaataa agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt
    14701 ctttaaatga attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac
    14761 ccataaaaat accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat
    14821 tttttacaac taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt
    14881 tgtcaaagtt ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc
    14941 cgccttcaac gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg
    15001 catttttgat tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa
    15061 gactttcggg aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat
    15121 tgattaatcc gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa
    15181 aaacaaccgt aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta
    15241 catttgcgaa caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa
    15301 atactgcttg gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat
    15361 aactcatggc aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt
    15421 ttttctttaa accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat
    15481 ggaaaactat aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat
    15541 tatatagacg tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact
    15601 taacgttcgt gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta
    15661 ggaatagctg attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt
    15721 tgaggaagga aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta
    15781 ccctcaatca agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca
    15841 atgccggtca tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt
    15901 accaacaatt agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc
    15961 ctaatcagca ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa
    16021 tcttatcgca aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt
    16081 tttttattga cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag
    16141 agaagaattt gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa
    16201 cagacaaacg ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg
    16261 tcgatgttcg gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc
    16321 aaaaagcaaa aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca
    16381 ttgcctttgg tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag
    16441 ctatggctaa cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca
    16501 atcaggaaga cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta
    16561 gattccacaa aaaagaggtt gtgacaat
//
LOCUS       DAGCYL010000047        16491 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.048, whole genome shotgun sequence.
ACCESSION   DAGCYL010000047 DAGCYL010000000
VERSION     DAGCYL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.048"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            19..1158
                     /locus_tag="MB216_001178"
     CDS             19..1158
                     /locus_tag="MB216_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBT4526026.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1151..1951
                     /locus_tag="MB216_001179"
     CDS             1151..1951
                     /locus_tag="MB216_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4526027.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1951..2778
                     /locus_tag="MB216_001180"
     CDS             1951..2778
                     /locus_tag="MB216_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4526028.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2756..3490
                     /locus_tag="MB216_001181"
     CDS             2756..3490
                     /locus_tag="MB216_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4526029.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            3590..4456
                     /gene="rfbA"
                     /locus_tag="MB216_001182"
     CDS             3590..4456
                     /gene="rfbA"
                     /locus_tag="MB216_001182"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBT4526030.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4470..5042
                     /gene="rfbC"
                     /locus_tag="MB216_001183"
     CDS             4470..5042
                     /gene="rfbC"
                     /locus_tag="MB216_001183"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBT4526031.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5064..6092
                     /gene="rfbB"
                     /locus_tag="MB216_001184"
     CDS             5064..6092
                     /gene="rfbB"
                     /locus_tag="MB216_001184"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBT4526032.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6190..7041
                     /gene="rfbD"
                     /locus_tag="MB216_001185"
     CDS             6190..7041
                     /gene="rfbD"
                     /locus_tag="MB216_001185"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBT4526033.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7076..9109
                     /locus_tag="MB216_001186"
     CDS             7076..9109
                     /locus_tag="MB216_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526034.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9153..10433
                     /locus_tag="MB216_001187"
     CDS             9153..10433
                     /locus_tag="MB216_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBT4526035.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10643..11449
                     /locus_tag="MB216_001188"
     CDS             10643..11449
                     /locus_tag="MB216_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4526036.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11461..12681
                     /locus_tag="MB216_001189"
     CDS             11461..12681
                     /locus_tag="MB216_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4526037.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12671..14257
                     /locus_tag="MB216_001190"
     CDS             12671..14257
                     /locus_tag="MB216_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4526038.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14296..16434
                     /locus_tag="MB216_001191"
     CDS             14296..16434
                     /locus_tag="MB216_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4526039.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALKMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         5228 a         2729 c         3505 g         5029 t
ORIGIN      
        1 attaaagtag gtgcccatat gcttgtttct tttagtatgg ttataagatt ttttctgaca
       61 attttattat cactgatatt aacacctgta ttcaaaatca tttcagtaca aacaggtatg
      121 gtcgacaaac caaatgaaag aagaataaat aaagttccaa tgccttcagc gggcggactg
      181 cctatcttta tttcatttgt tatatcgact ttattcttat tccgtaaaat tatccctcaa
      241 tcgtatattg taccgatctt gattgcttct ttagtaatca tagtcacagg agtgctcgat
      301 gataaatatg agctgacacc taaacaaaaa agtataggga tcttgattgc ttctcttatt
      361 atttattttg tagcagatat acgtattgat tccttcacgc ttccttttat tggatatatc
      421 caactaggct ggcttagttt tcccgtaacg attttttgga tcttcggtat cacgaatgcg
      481 gtcaatctga ttgacggttt ggatgggctg gcggcaggga tttcattgat cggtttaatt
      541 acgataggaa tcattggata ttttttcctc catgcttcta cagtgtatat tccagttgtt
      601 attttctgtc ttgtagcaag tattatcggt ttttttcctt acaattttta tccggcaaag
      661 atttatttag gcgatacagg agctctattc ctagggttta tgatggccgt cctttcacta
      721 caagggctga agaacgtgac ttttgtttca tcgatttctt tgctgatcgt catgggagtg
      781 ccggtaacag acacgttttt tgctatcatt aggagaaaag caaaccgtgt ttctttttct
      841 acagcagata aaaaacatct tcatcaccga ctaatcagtt taggctttac tcataaaggt
      901 gctgtcctga cgatttatgc gatggctttg atgttttctt tcacagctat ggtcatgaat
      961 tatactggaa gaatcggaac gatcatattg attattgcca tgttgtttgc tgcaatttta
     1021 ttatttgaat tgattggtct gatcaatgag aaacatcagt ttattttaaa aacattgcga
     1081 tttttaggga ataaagagta ccgtacgcaa gtaaaacaga aatatttgaa gaaatggaac
     1141 cgtcatatag atgaataaca aattaaacca gccgatcctt attagtattg tgacggcaaa
     1201 cagtgataaa atttttcgga cactagataa ttttatttca actgtaaaag atgaaacagc
     1261 agtaaaaatc cgaatttttg acaacaattc acttcccgag tacaaaaaaa gactaacaga
     1321 gtataggaaa tatccctaca tcgatatcta ttttcatgat gaaaattccg gatttggtta
     1381 tggacataat cacaatctga tagctagcga ttctcgctat ggcattattt gtaacccaga
     1441 tattttagtg gatgaagaga cagttacagc attggtcttg ttattgaaag aacatccgga
     1501 atgtgcgatg ttagcaccta agattcttaa tgaagatgga acaactcagc acctcatccg
     1561 tgagcagctg gctgtttttg attacgtttt gcgctttgtg cctttcaaat gggtcaaaaa
     1621 aaagtttgac aaacgactgg ctgtgtttga atgccgagga ttgcctactg atgctaatag
     1681 ttatgtacgg atgatctcag gtagttttat ggtagctgat attgataaat ttaaagagat
     1741 aaaggggttt gatgagcgtt tcttcatgta ctttgaagac aacgacttgt gtttgagttt
     1801 tgaaaaagca ggttacaagt tattgtacac acccttttat gcagttatcc atttttacgg
     1861 gaagggtgcg catcgaaatg caaaactgtt tcgcgtgttt ttgacttcta tgatgaaatt
     1921 tttcaataag tgggggtggc gttttttctg atgaaaccag aaatattagt agtcgttgtg
     1981 ctttatcagc aactattcag tcaatctcca gcttatggac cattaagcaa agcacttagc
     2041 gaaaagcaaa ttcagcttat tatttacgat aatagtcctc aaaggcagca aaatgagctg
     2101 tttgaaaaag aaaatatcgt ttatcatcat gatccgcaaa atcctggttt agcggtggct
     2161 tataattttg cgttgtctca agcagatgaa ggaacacgtt gtctcgtcac tctggatcag
     2221 gatacgaggc tagtcgacga ttattttgaa ctattgcgta aagtaacctt tacagatgaa
     2281 tgtgttgctg cagttccgat gattttttct gggagccgac aagtttctcc cctatattca
     2341 gatcgttata tcaatcgtac agctcaagca gtagaagttg aaggcccaac agctgaacga
     2401 atcatggcaa tcaattcagg tgttgcctgg tctgtcaaat ttctaaaaaa gattggcggg
     2461 tttaatcctg ctttttcttt agattttttg gatcattggc tgttttggaa agtggatcag
     2521 ttggaaaaaa ctgtggagat ccttcctgta cgaatggaac atgatctttc ggttttagac
     2581 tatgaaaaag tttcaagtaa tcgataccgt tctattttac aagcagaaaa tcgattttat
     2641 catgaatatg atcaaaggca tttatccagt caccgacgac aattattgtt aagaacagct
     2701 aaacagttct tgaccgtcaa aaaccggcat atctggcgga tgactttgcg ctcatatgtt
     2761 aagaattgga aggtgtgaat tcgtgcgttc ggtttgtata gctaccttta atggaggcag
     2821 atatttaaga gagcagttag atagtatttt atctcagctt tcagcagaag atgaagtagt
     2881 catttctgat gatgggtcag tagatgatac atgggatata ttaactgaat acgcagaaaa
     2941 agataagcga atccagcttt tttcggggcc tcgtcaagga ttgatcgcta attttggtta
     3001 tgcgatccag cagacgaaag gagatctaat ctttttggca gaccaagatg atgtttggtt
     3061 ggagacaaaa gtgaaaaaga tcactgacta ttttgagcag aatccggatc agctagtagt
     3121 agtttctgat ttaatcattg tggacagcga gctgcaagag atccatgatt cgtatttttc
     3181 gtttcgaaat tccaaagaag gatggcttaa taatgtgata agaagtcact atattggtgc
     3241 tggtatggct ttccggtctt ctttaaaacc cgatattttg ccgatcccgt cagaagtgcc
     3301 gatgcatgat atgtggattg gtttgctggg aggaaagaag actgggttcc taagagaacc
     3361 gttaactctg taccgaagac atgatttaaa cgcgagtgag attgaaacac attctaaatt
     3421 acggcaaaaa atttcttggc gtgttcatct gattacggcg ttgatcaaac gaaaatggtt
     3481 taaacgttga taaaagatgt ttaaatcctt aatatttcca tgacaaatat aacaattgat
     3541 gagataataa caaagtgaaa tttgaaatca aaaaggagag cagatatcca tgaagggaat
     3601 cattcttgct gggggcagcg gaacacgtct gtacccttta acaaaggcaa catcgaaaca
     3661 attgatgccg atttatgaca aaccaatgat ttattatcca atgtctactc tgatgttggc
     3721 gggaatcaaa gaaattctga ttatttcaac gccacaagat acaccacgtt tcaaggaatt
     3781 atttggaaac ggaaatgatt taggactgca gatcgaatat gcagtacaag aaagtccaga
     3841 tggattggca caagcgttta tcattggaga agaatttatt ggagatgata gtgtctgctt
     3901 ggtattaggg gacaatattt actatggtgg cggtctgtct aaaatgttgc aacgagcagc
     3961 ttcaaaagat acaggcgcga cagtatttgg ctatcatgtc aatgatccag aacgctttgg
     4021 tgtagtagaa tttgatgaga atatgcaagc tttgtcgatt gaagaaaagc ctgctcagcc
     4081 aaaatcaaat tacgctgtaa ctggccttta tttttatgac aatgaggtaa tttctatcgc
     4141 taaaggtatc aaaccttcag aacgtggaga actagagatt acggatgtca ataaagcata
     4201 tttggataaa ggaaaactat ccgtagaact tatgggacgc ggatttgctt ggcttgatac
     4261 aggaacacat gaatcattgc ttgaagcatc tacctttatc gaaacaattg aaaaacgtca
     4321 gaacttaaaa gtggcttgtt tggaagaaat tgcttaccgt atgggctaca tcgataagga
     4381 acaattgctg gcccttgcac agccattgaa aaaaaatcaa tacggtcaat atttattgaa
     4441 tttggccgct gaataaggag tccttacaga tgaaagtaac agatacaaaa ttaacagatg
     4501 tcaaaatcgt agaaatggac gtctttggcg atcatcgcgg tttcttcaca gaaagttatt
     4561 caaaagcaaa atttgcagaa catggattga aatatgactt tgttcaagac aaccattcac
     4621 tatcagctga agctggtgtc cttcgggggc ttcatttcca aaaaggagaa gctgcacaga
     4681 caaaattgat tcgggtagtc actggagcag ttttagatgt agtagtggat atccgcaaag
     4741 gtagcccgac ttatggtcaa tgggacggat ttatcttatc tgaacataat catcgacagt
     4801 tgcttgtacc taaaggatat gcccatggat tcgttacttt gacaccaaat gtgaacttcc
     4861 tttataagtg tgataattac tataatgcag aagcagatgg gggcatcgca tttgatgatc
     4921 cagatttagc gattgattgg ccaattgatc catcaagagc gattatgtcg gaaaaagatc
     4981 accgtcatcc gacattaaaa gaatttgaag cagaaaatcc atttgtttac ggagaaatct
     5041 aagaattaag gagaaatttt cttatgaaaa acatcattgt taccggaggt gccggattca
     5101 tcggctctaa tttcgttcat tacgtggtaa acaatcatcc agaagtgcat gtcactgttt
     5161 tggataaact gacttatgca ggaaacaaag agaacttggc cggacttcct agcgatcgcg
     5221 tagaattggt cgtaggagat gttgcagatg cagaattagt ggaccgtcta gtaaaagaag
     5281 cggacgcagt tgttcattac gcggcagaat cccataacga taattcatta aaagatcctt
     5341 tcccatttgt acaaacgaat atcattggta cgtatacact tttagaagct tgccgcaagt
     5401 atgatgtacg ctatcatcac gtatctactg acgaggttta cggcgatctt ccattacgtg
     5461 aagacctgcc aggacatggg gaaggcgaag gagaaaaatt cactgcggaa acaccatata
     5521 atccttcaag tccatattct tctacaaaag ctggttcaga tctgttagta aaagcatggg
     5581 tgcgttcttt tggcttacgt gcaaccattt caaactgttc aaacaactat ggtccttatc
     5641 agcatattga aaaatttatt cctcgccaag taacgagtat cttaagcggt attcgtccaa
     5701 aattatatgg cgaaggaaag aatgttcgtg actggatcca tacgaatgat cattcttcag
     5761 cagtgtggct gattttgacg aaaggcagaa ttggcgaaac ctatctgatc ggtgcagacg
     5821 gtgaagaaga taataagacc gtgatggaat tgatcttaga aatgatgggc caaccaaaag
     5881 acgcatacga ccatgtaaac gaccgcgcag ggcacgattt gcgttatgca atcgattcga
     5941 caaaattgcg agaagagcta ggttggcagc ctgagtttac gaacttccgt gatgggttgg
     6001 ctgacacgat caaatggtac gaagaacatg aagactggtg gaaaaaagaa aaagaggccg
     6061 ttgaagcagc atatgccaaa aatggacaat aatacagagg aaatttcact gtattaatgg
     6121 aaaaacacat gtgaggcagg cgctcgcctg tctctttttt aaaataaaaa tcaaggagcg
     6181 gataaccata tgtttttatt aactggagga aatggacaat taggtacaga attgcgccat
     6241 ttattagatg aaaaaaatct gccatatgtt tcaacagatg caaaagactt ggatatcaca
     6301 gatgctgaga aaacaatggc ttatattaca gaattaaaac ccgatgttat tttccattgc
     6361 gcagcttaca cagcagtaga taaagcagaa gaagaagcaa aagaattaga tgagaaaatc
     6421 aatgtcgatg gcacaagaaa tgtagcactt gcagcaaaag ctgtgaatgc acaattcgtc
     6481 tatatcagta cagactatgt tttcgatgga aaaaacaaaa atgtagaata cgaagtagac
     6541 gaccaaacga atccactaaa tgaatatggt cgtacgaaat tattaggcga aaaagcagta
     6601 gaagagattt tagaagacta ttatatcata cgaacttctt gggttttcgg gatctatgga
     6661 cacaatttcg tcttcacgat gcaacgccta gcggaaaccc gcgacaaact gactgtagta
     6721 aatgaccagt ttgggcgacc aacttggaca cgtactttgg cagaatttat ggtatatgtc
     6781 atcgaagaaa aagcaccatt tggtatttat catttgtcga acgaaaatag ctgttcttgg
     6841 tatgagttcg caaaagaaat attgaaagat acagatgtag aagttgcacc tgttacgtca
     6901 gaagaatttc cgcaaaaagc aacacgtccg cagtattcag tcatgagcct gaaaaagaca
     6961 gaagcgttag gttttgtcat accaacttgg caagaagcac ttgcgcaaat gctggaagct
     7021 gccaaaaaaa tgaataagta atttcctttt tatcaaatag aatttggagc atgatatgga
     7081 aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa gcgaatcagg cagattcgaa
     7141 tttggtgctt gacctgtttc taaaactccg ctatcttatt ggcgttttag tcatcgtggt
     7201 catcgttact tttaatttaa acggaagttc acttggtgtt tgggataaat atgtgtcgca
     7261 aagagacgat gggaaaaaaa ctgatgtgat ttttggacag aatcgggaag tgcggtcaga
     7321 cgaatggctg gtgcagacac ctttttacct ctcacaagct gaagaggact tttcgttggt
     7381 gaacgaggat tattcattaa acggacaaaa catgatcatt gcgtataatt ctcctgtaaa
     7441 ggatattacg gttatcggaa aaccattcaa ctggggattc tttttcctcg gaagagatcg
     7501 aggactttcc ttttattggg cgatgaaatt agtcgtcatg gtcttgcttg gattcgaagt
     7561 actgatgata ttaacgaaga ggaaaaaagc tctatcgcta atcggtgcat ttgcgctgac
     7621 attttctcca gcagtccagt ggtggttcat gcaacatgtg ggcgatttga ttttctttac
     7681 tttgggactg atgattgctt tttatcatta tttctacaat catgataaaa aatggctacg
     7741 aacgttgatg atgcttttgg tggttatttt cggtattggg tttattttag tgatttaccc
     7801 agcccatcaa gtgatgctgg cgtacttgct gattttttat tttgttggcc tgcttattta
     7861 ctatagcaag aaagtttcat gggatctgtt ggatgtagtg ttgattggag gggcagtttt
     7921 atttattggc ggagtcatgg ttcatttctg gctaacgtca aaagacgcat tgttagcttc
     7981 tttgaatacc ttatatccag gaaaacgggt aagtactgga ggaaagtgga cgattggcga
     8041 atttttttct ttccttacaa attggaaaat tccttttgaa gatatcaact attcgaataa
     8101 ctctgaagtg gccctgttct atcacttttt tccaactgta ttcctagcaa gtccatttgt
     8161 gttatttggc aaaaaagata gcgaacaaaa gttactggga cgtattttga tgattttttg
     8221 tctgttttct atcttttgga ttaccgttgg gttgccaaaa tggctagcag aaatcactct
     8281 gctgtcttat gttcctccat taagagccat ccttactttt agttttgcgg cttgtttatt
     8341 atcgatctgg tttatcgctt atatttggag agaagatgtt attcctattt ggtataaatt
     8401 cattttgttg gcagtaaatg catggtctta tttctatgcg attacgcatt cgaaaatgga
     8461 taattatttt tcggattttg aaaccatcgc agttgctgtg ttgggaacag tgattttagc
     8521 ttttgcatta tttggctgga gacgcttgtt ttacctcgct tttgtcgcct tagttatcgt
     8581 atctggcttt ttcgtcaatc cagtcgtaga aggtacagga gcgatttttg aaaaaacact
     8641 ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt aaagtttggt tgtctgaaga
     8701 tgaattatat aacttcaccc caacattggg agtgaaatcc ttcaatacag tacgctttta
     8761 tccagatata gctgcatgga agaaaatcga tccgaaagaa gaatacgaga agtattataa
     8821 tcggtatgct catacaagag cttttatcgc taaggaagat acaaagttca ttttagatcg
     8881 tccagatatg ttttcggtca cgttgaattt tgaagacgca gaaaaattag ggatcgacta
     8941 tgtgatcagt aaacgaccat taactgatta caaccaatta tatgaagcgc agttcacgca
     9001 gctatatgga ccggataaag acggttatat gatttttaaa gtagaatatc cggattatcc
     9061 agatgtacca caaacacaaa ctcaggaaaa tcagctagtc gtacagtaat aggctagctg
     9121 agaataatcc tgaaatatta gtagaaagga gagtgaagat aattcaagca atcgtacaaa
     9181 aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt gctgacgcta gtcttttttg
     9241 tttggacagc aggggtaggt tttccagcaa ctttgtttat atataacaat agttcattac
     9301 ttatcgcttt tagtgtggct attttgctca ttttaaatgt ttataaagca aaaggcgtcg
     9361 attttcttta tctaggtgtt gcgcttgccc ttttcatttt cttttcttac tttcgggaat
     9421 taagaaatga gtctttattt ggagacttac tgtttccttt gattatttta ttttgtttag
     9481 taattaaatg gatcgagtgg acaaaatggg atcgcggtat ttatctgatt gtctttacgg
     9541 tcttcctcgg aatcacgtta tatcgtgtat ttacagagat ccaagtacca gaaggacaaa
     9601 gcatctgggc tccaggcaat tcgctttctg atatttggat caatacgaat acaatcggaa
     9661 gttctttgat gactcttgct ttattgatca gtggatttgc cagttctttt gaacgatggt
     9721 atgttcgaat catcggtggg atagcagtcg ttgcagcctt tttaggtatt tgggtctgcc
     9781 aatctagagg tgcattagtc gcgttgattg tttttgcgat attggatctt ttacctaaat
     9841 cgttgatgcg tgtcattcgt gcgccattta ttgcctacac ggttacgatt cttttagctt
     9901 tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa tttgtttacc ggtcgagaag
     9961 atatttggca taaattctat cagacgctag gagaaaaatc tgaacaagtt ttactaggaa
    10021 tgaaaacatt tatcttccag cgaggaaacc aatttttagg aaaccacaat agttataatt
    10081 ctattttgaa tatctatgga ttgataggat ttgggattgc tgctttgctg cttatccttt
    10141 ttattggtcg cttaacgttg aaagcagatt tatccaacgg gcagatgact tttatctggg
    10201 ccttttttgc ggtgatgatg cagtcattta tggaggatac attgacttca tacccatggg
    10261 ttccgattat ttatatcctg ttggctatgg ccgttcatcg atatgatgaa ccagaagtta
    10321 cggaggaatt ctatgttatg gaagaggcat atgacgatat gacttatgat gatacatctt
    10381 atgaggaaca accagatagc gctcattctt ccaggatcca acgctatcat tgataggcac
    10441 aaaatacgag agaggctgtg acaaccgtcg cagccttttt tggtttattg caattgtcta
    10501 gcatcttctg taaaatagaa acgttagaga aaatcttgta tcatagaaaa ttaagaaaga
    10561 aataagaacg cagctcggta ccaaaaatat ggtaaaatgg cacttgactg taattcactg
    10621 aaaatcgctt ggaggaacac aaatgtttaa agatttgtat ttatttatga aggacattta
    10681 tcagaacaga aaattactgc tacagttttc gatcaatgac ttcaaatcaa gatatgcagg
    10741 atcgttttta ggaatcttat gggcgtttat caatccgcta tttactgtat tgatttattg
    10801 gctggtattt ggtgttggac tgaaagcaca aatgatcgat gcaaaatacc catttatcgt
    10861 atatttagtt accgggatga ttccctggtt cttcttctca gatgcatttg catcaactac
    10921 ggttgttttt cgggaatata cttatttagt aaagaaagtc gtattcaata ttcgtatctt
    10981 gcccacgacg aaaatcctat ccaatctgta cacccattta ttcttcattc tgattggcat
    11041 agctgtcgcg cttataaatg gtatatatcc ttcagcaatg tctttacagc tgatttatta
    11101 tttattttgc atgtgtgctt tcttgacagg attgacttgg ctgacggcat cgatccaacc
    11161 atttttacct gatatcatgc aattaattac gatcgtgctg cagttgatca tgtggacatt
    11221 accgatatta tggagcccta atcagtttga ttcaggtctt gtaagcatat tgaaattaaa
    11281 tccgctgtac tatgttgttc aaggttatcg tgagtcattt ttaagtgaag cttggttctg
    11341 ggagcatgga acatatactt tgtatttctg ggttttcacg ttagtaatgt tcgtaatcgg
    11401 ctctgcagtg ttccgtagat tgaaaccaca tttttcagat gtgctgtaag gagaatagct
    11461 atgacagaat atgcaattag actaaaagat atcacaaaat cttataatat gtacgcaaaa
    11521 ccttcggatc gatttcgtga agccctcaat ccttttaaga aatcctatca tgatgtattt
    11581 tatgcgttga aaaacgtgaa tgtagaaata aaaaaaggtg agatgatcgg tttcgtggga
    11641 gaaaacggat cgggaaaatc tacaatgttg aaaatcatta cgggagtact gacaccaaca
    11701 tccggcacga tggaaatcga aggaaaaatc tcagctttgc tagaactagg atcaggattc
    11761 aatcctgagt attctggcta tgaaaatatc tatttaaatg gaatggttct aggattttca
    11821 agagaagaag tcgatgaaat ggtcgatgat atcattgaat ttgctgacat cggcgaccat
    11881 atttatcaac ctgtaaaaac ctattcaagt gggatgttcg ttcgtctagc ttttgctgta
    11941 gcgatcaatg tgaatcctga tattttgatt gtggatgaag cattggcagt aggagatctt
    12001 gaatttcaac taaaatgtat ggaaaaattt acagaaatca agaactcggg aaagacgatt
    12061 ttattcgttt ctcatgatat tagttcgatt cgccgttttt gtgatcgttc ctattggttg
    12121 aaaaatggaa aagttgtcga atatggtgat acgatggaag taacgacgaa ttacgaaaac
    12181 tttttgaaga aaaaatcagt taaaacagta gatcgaaata aaaaccaagt agaacgcttc
    12241 gcagctccag atatcgtaga cgtgacgaaa gccgaattgt tggataaaga taaaaatcca
    12301 gtggatatgc tggaacaagg agaaaaacta tacgttaaag taacctatga agtaaaagac
    12361 gatacaatca aaaatccagt tttaggaatt gctctgcgta cagtcgacaa tttttatgtg
    12421 tgcggactca atactctact agatggtatc aagatccctt ggaaaaaagg tgagaatatc
    12481 ttttatctgg agtatgaaaa gttaggtttg ttagctggtg aatattattt tgacgttgcg
    12541 gtctttgaag aaaatgcaac ggttcctttg gtatacaaga caaaatacat gaacttgttt
    12601 gtcagcggtt cttatatcgg agaaggcatc gtggtcttag accataaatg ggaagaaggg
    12661 actcatagca atgaaatatg attttgaaat ggacctagat gaacaaagca gtgtgggaaa
    12721 aattgccgca cagatcaaac caggaagtaa agttttagag tttggtcctg gaaacggccg
    12781 attaacgaaa catttgatcg gagcaaaaca atgtgaagtg agcattgtcg agctagataa
    12841 ggaattgttc gattttgtca gcgagtttgc ccaagatgga ttttatggag atatcgaaag
    12901 ttttgaatgg gcaaactatt atgctggaca aacatttgat tacgttttgt ttgccgatgt
    12961 tttagaacat ttggtagatc ctggaaaaac gttgaagaaa gttagagaat ttttgaatga
    13021 agaaggagaa attctaatta cattccctaa ccttgcgcat aattctgtca tgattgattt
    13081 gttcaataat caattaccat gggcttctta tggtttgcta gatgaaaccc ataattcttt
    13141 ttatactcat gatggcttcc aaaaagtttt tgaaaaagca ggattataca tcaatattga
    13201 agattatttg tacttagctg taggtgatac agaattaaaa tcaacttatg aagaattgcc
    13261 ggaagctgta cgttatgatt tcaaaatgcg accatttggc gaagtctacc aatacttctt
    13321 ctcattgatg aaacatccag tagcacaaag ttccattgcg cagccgcaaa attctaatta
    13381 tgtgaaagta ttagaagtaa tccagcaaac gaaacaagat gaaataattc aacaaatacc
    13441 attcaataat ttcacagggg aaaatgagac actaagtttc catgtatcag aattagcaga
    13501 acgaatggtg tttcgcttcg cgcagcaacc tagctttatc gaattcagcg cagaagctgc
    13561 tggagaaaaa ctcacattta tcgattcaaa tgctgtagtc aaaacagtaa atgattgtta
    13621 tttgtttgat ggtaaagatt taccagagtt ctttttaacg gatattgcag ggaaagaagt
    13681 gactatccat tgtcactatc gttttatcgg agaattgaca ccaacaatga aagaattgct
    13741 ggaaacaatc cgtccaatgg ctgaagttac aaagcagctt tctgagaaaa atgatgaatt
    13801 agaaagagaa aatcattatc ttattgagaa taatacaaga ttagatcaga cgttgaaaac
    13861 aaccataaat cgctattgca cgttgttgga aagtgatgaa tggacaatca aacatagatt
    13921 agggcgacgt aagaaagaaa catcgaaaaa aatccaagaa aaagagcttt ctctatgtat
    13981 tgacgaaaaa atctgggatg cagaaacaaa gatattgaaa atcatcgggt ggggaatcgc
    14041 taattcggat cgccaaccat tgtcctataa gttatcagcc gaccaatcac cattttttga
    14101 agcaattcct gttttacgag aagaagtcaa tcaagcaaaa cagctagctg aaggagcaaa
    14161 agcaggattt gaactgcgga tcctttgcga gaaggaacgt tcattcttag ttgaagcagt
    14221 ggctcaaaat ggtcaatcat ggatcattga aatgtaaata atgattaact tataaagata
    14281 aggagtaaac gaatcgtgca aaaaaaagaa attgccgtat ttatcgatca aatcactcgt
    14341 gaacgggcca ctggcgattt gcttattgtg ggctgggcaa ttgatgaagt gaccaaagaa
    14401 atccctacga taaaggtaga aaaagaaaat gtgattgctg aagcaacaca tgtcgtaaga
    14461 ttggatatca atcatttata taatcttgat gttaagaccc aaagcggttt taaggtacgt
    14521 ttatcaggga aaatgagagg aaaagcaatc ttagacttcc aaacagcaag acatcaaaat
    14581 ggcatagctg tgaaattaaa cggtaaatat ccttatgatg acggcattga atcttcatgg
    14641 gaaagaaaaa aaagattact gaaaaaaggg ataaattatg caaggactca tggcgtaaaa
    14701 aaagcaatcc gccgggtcaa actggaattg aaaccaggat cgatcgacta tgcagagtgg
    14761 atcagtcgtc atgagaaacc tgatctgaaa gagcaaagga aagaagtaca aggatttgcc
    14821 taccgcccat tgatttccat acttatgcct gtctataacg ttgaaatcaa atggctcgaa
    14881 aaatgcattg attcggtact tgatcaaacg tatgatcact gggaactatg tatcagtgat
    14941 gatgcttcta ctgatccagc tatccgaaag tgtctagaat cctatcaagc aaaagatgat
    15001 agaattaaag tcgttttccg acaagaaaat ggacacatca gtctggcaac taattctgca
    15061 ttgaaaatgg cagaaggaga gtttatagcg ctcttagata atgatgacga attacctcca
    15121 tttgccttat atgaagtggc aaaagtgcta aacgtacatc cagaactgga tttgatctac
    15181 agtgacgaag ataaaatcga tgcagatggg aatcgctttg acccgcactt caaagcagac
    15241 tggtctcccg atacattgat gggaaataat tatatctctc acttaggcgt atatcggaca
    15301 agcattgtta aggaacttgg cggtttccgt aaaggatatg aaggctctca agattatgat
    15361 ttggttcttc gtgtgacaga acaaattcct gcagaacaca tttaccacat tgaccgtgtc
    15421 ttgtatcatt ggcggacgat cccgggatca actgcaagca atggagaagc taaaagctac
    15481 atctatgatt ctggagtgaa agcattgaca gatgctctat ctagaagaaa tatcaaggga
    15541 agcgtacatc ctggcagaat ttctggtttt tatgaaatca cttatgatgt tttgcaagaa
    15601 gacttggtca gtgtgatcat tccaactaag aatggctatg aagatctaaa aacttgtgtg
    15661 gattcgatca tcgaaaaaac gtcttatcca aattatgaaa tcattattgc agataatgga
    15721 agtacagatc ctaaaatgca agaattattt gcagaataca agcatcagct gaaagaccga
    15781 tttatcgtcg aattaattga cattccattc aattattcac gtatcaacaa tctggcagca
    15841 gaaaaagcaa acggcaaata ctttttattc ttgaataacg atacagaagt gattgaacct
    15901 gactggatga cagcgatggt ttcttatgca cagtttgatc gaatcggctg tgtaggagca
    15961 aaattatttt atccggatga tacaacgcag catgcaggtg tattattagg gatcggtggc
    16021 gttgcaggac atgcattgaa taactatgat cgaacacatt gtggttactt tggccgattg
    16081 gtcattgacg tcaattactt agcagtcact gcagcttgta tgatggtcaa ggcagctgac
    16141 ttcaatgctg tcaatggatt tgatgaaata ttagaagtag cattcaacga tgttgacttg
    16201 tgcttgaaag tttatgaact gggtcgttac aatgtctatg cacatcaagc agaattgtac
    16261 catttcgaat ctaaatcaag aggttatgaa gatacaccag aaaaacaaag acgttttgct
    16321 ggggaaatca aaaaaatgca agataagtgg ccttcttata ttgctcatga cccattttat
    16381 aacgataatc taactaaaga aggaatcggc gatttttcgt tgagacctga ttgattaaaa
    16441 gaactgtatt ttcattttta ttaatgaaaa tacagttctt ttaatttaat t
//
LOCUS       DAGCYL010000048        16446 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.049, whole genome shotgun sequence.
ACCESSION   DAGCYL010000048 DAGCYL010000000
VERSION     DAGCYL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.049"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..477
                     /gene="scm"
                     /locus_tag="MB216_001192"
     CDS             <1..477
                     /gene="scm"
                     /locus_tag="MB216_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBT4526040.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(632..1618)
                     /locus_tag="MB216_001193"
     CDS             complement(632..1618)
                     /locus_tag="MB216_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBT4526041.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1641..2207)
                     /locus_tag="MB216_001194"
     CDS             complement(1641..2207)
                     /locus_tag="MB216_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBT4526042.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2221..2637)
                     /locus_tag="MB216_001195"
     CDS             complement(2221..2637)
                     /locus_tag="MB216_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBT4526043.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3094..5028)
                     /locus_tag="MB216_001196"
     CDS             complement(3094..5028)
                     /locus_tag="MB216_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4526044.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5309..6265
                     /locus_tag="MB216_001197"
     CDS             5309..6265
                     /locus_tag="MB216_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBT4526045.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6355..7458
                     /locus_tag="MB216_001198"
     CDS             6355..7458
                     /locus_tag="MB216_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBT4526046.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7471..8424
                     /gene="menA"
                     /locus_tag="MB216_001199"
     CDS             7471..8424
                     /gene="menA"
                     /locus_tag="MB216_001199"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBT4526047.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9238..10584)
                     /gene="gor"
                     /locus_tag="MB216_001200"
     CDS             complement(9238..10584)
                     /gene="gor"
                     /locus_tag="MB216_001200"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBT4526048.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10718..11971)
                     /locus_tag="MB216_001201"
     CDS             complement(10718..11971)
                     /locus_tag="MB216_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBT4526049.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11977..12900)
                     /locus_tag="MB216_001202"
     CDS             complement(11977..12900)
                     /locus_tag="MB216_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBT4526050.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13121..15613)
                     /locus_tag="MB216_001203"
     CDS             complement(13121..15613)
                     /locus_tag="MB216_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBT4526051.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15620..16087)
                     /locus_tag="MB216_001204"
     CDS             complement(15620..16087)
                     /locus_tag="MB216_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBT4526052.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16223..16296)
                     /locus_tag="MB216_001205"
     tRNA            complement(16223..16296)
                     /locus_tag="MB216_001205"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16260..16262),aa:Arg,seq:acg)
     gene            complement(16354..16427)
                     /locus_tag="MB216_001206"
     tRNA            complement(16354..16427)
                     /locus_tag="MB216_001206"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16391..16393),aa:Pro,seq:tgg)
BASE COUNT         4852 a         3445 c         2927 g         5222 t
ORIGIN      
        1 agcacggaca cgtcagcttc aagcgacagt acggatacat ctaccacaac aagtgatagc
       61 acggacacgt cagcttcaag tgacagtaca gatactacct ctaccacaag tgatagctca
      121 gattcatcta ccactacaag tgatagcacg gacacgtcag cttcaagtga cagtacagat
      181 acatcctcta ccacaagtga tagctcagat tcatcaactt caagcgatag tacagacacg
      241 tcgtctacca caagcgatac acgtcatgca agtgtctcct tctcaaaaac aagttcggat
      301 aacaggacga actttaactt aactaataaa acaagcaatg acaatggaaa caaatcaaaa
      361 agagcacttc ctaaaactgg aagtcaatcg aataattgga taacactcgc aggcgttata
      421 ttacttgtaa tcggtttaag aatcactttt atgagttata agaaatcgaa aaggtaaaaa
      481 aatattcaag ataaaaaaga gcaacgtcgc taacggacgt tgctctttat ttttgctttt
      541 tatcataaat acaagaacta caattcaatt tgtcaaaaaa cacgtagatt tccttctgag
      601 aaatttattt acgtatcgaa cctttcccta ttcagtcttt gcgatccaaa atcagttggg
      661 taagggaaaa gagattctct tttgttcgaa gagaggtggc aggtaatttc tttatttcct
      721 tcaaagcctt ttctgtgtaa gaggccgcta acttcctagc ttcttccacg cctcctgtat
      781 gttcaacgat tttgcgtacg gcgtcgacat cttcttctgt cattgccgct ttcttctcta
      841 aataaggtag tagctttggt ttgttttttt gcaaagagta gatcaacggt aaagaataga
      901 ccccttgacg catgtcttct agtacaggtt tgccgatttc ctggctctct tttgtataat
      961 ctaaaatatc atcgataatc tgaaaggcca tcccgataga aagtccgata tctcctgctt
     1021 tttttgcgaa gcgctcgctt gttccacttt caaaagcgcc aacagaacaa cttaattgga
     1081 acagttcagc tgtcttacct gaaatatttt ccaaatattc ttttacagaa agttcataat
     1141 tatagcgatc atccatctgc cctaattcgc cattcaatac tttttccata ctgcgagaat
     1201 tcaattggag acttttcaag gaagtggagt aatcagacaa tagtttaaaa cagcagacaa
     1261 ataaatagtc tcctgcatag acagaaacga cattaccaaa ctgactacgg atattaggct
     1321 gaccacgtcg cagatccgct tcatcaacga tatcatcatg gatcagtgtg gccgtatgga
     1381 gcatttcgat agaagctgcc aaagcgatgg ctttgtctcg atcttgctct ggtccaaatt
     1441 gtgagaacaa tagctgatag gcaggacgca acagctttcc gccggcatgg atcgtttctt
     1501 tgatggcttg ttcgacaggt ttgtttttca ggcggacagt ttcttccatc cgttttagtg
     1561 tttctgtcaa ttctttactt aaagcaggat agttttccca cattggatgt agcttcataa
     1621 aaaaactcct tgtgtgacga ttattttttg gtaaacattg ggctccattt ttgtttggtt
     1681 ccctgctcat aatctttttg gaatcgttga agtgtctgca catgtcgtaa aagataattc
     1741 gctgcgatcc cgattgcaat ccctgataaa atccctaaaa ctgaaaggac tggcaagtac
     1801 aacatgactg tccatgattg tgcaatgaaa gaagccatca aaagctgtcc gacattatgc
     1861 atgaaaccgc cggccgcact aattccgatg atactgacac gttttggtcc taattgcttg
     1921 accaataaca ttccaaaata actaagcaaa gcaccactca tactataaag gaatgtggaa
     1981 atcgttccgc ctagcaaagt agtcaggaat agtcgtagcc aaagtagtaa gaagctgtct
     2041 tgttttttca tggtaaaaat ggcaacgatc gtgattagat tggctaagcc tagttttgca
     2101 ccaggcgcaa aagaaaaagg ataagggatc atattttcca ataaaccaat gatcactcct
     2161 tgtgcaacaa gtagcgaaat ataaatcatt ttttgtaatt tactcatggt gtgtgaaggt
     2221 ctaataaatc agactgtctt cgtcacctcc atctgttgaa cggacttcga tcactaattt
     2281 atgtggcaaa cagacgatgg tttcgccatt tttcgttgcc cagcctcgac gtacgcagat
     2341 ctggtcatcg caatctgctt cttttatccg tactcgatcg ccatctatct cgatcaagtt
     2401 ataatctccg tgttcatctt tgtaagtata tgtgttgctc tcttctccag ccatcaaagg
     2461 gaatgttttg atttctgtgc catccacacg cagtaccgct tctttttctg ctgttgcggt
     2521 gttttgctga agaccaaaaa tcatgactgg taaaaaagaa agaacagtca aaagcaaaat
     2581 aataaaaaca tcccaaggtt tgagcagact tttttcagca aattctttaa ggctcatttt
     2641 tgttctcctt tttccagatt aactcaccca attttagcat aaaaacaaga ggttgtgaca
     2701 cctgtgtctt gctttgagta ataagaacga agaatagaaa acagctttcc atgttttcta
     2761 attctttggg cttattaccg agaagcagca ggtggaacac cgtttataac aagaggttgt
     2821 gacagaagtg ggcaacttca agaaataaga aggaatttac gaaaattact tttcaagttt
     2881 ttgtgaaatt tcggcttatt tttcgaagaa gctacttctg gaacaccgtt tataacaaga
     2941 ggttgtaaca gaggttgtga cacctgtgtc ttgctcatgg aggcttatct cgggcatctt
     3001 attcctttaa ttttacataa aaaggaggct gtgacggttt gtctcagccc ctttctattg
     3061 aaatcttgca ttcaaataac gttctttatc tatttatttc ttgccgtact gggaacttgg
     3121 tctaccgtac caccaattac ctagtgtacg ttgtagccat ggaattaccc agcgatccaa
     3181 cccaatcgca cgtccggaac cgttcatcaa tgcgaaagca acaaaaatga accaaatgtt
     3241 tacccagtaa aacatgcctg ataagcagaa cgtgatcacc aatacaatcg ttgtcgcact
     3301 gcatagccat gtgaataatc cagcaatcaa tgctaatgcc aaggcaattt caaagaatac
     3361 catgaatttt tggaagaaca ttgctacatc attgtttggc atgatcactt gcatgatctt
     3421 tgtgaaccag tctggtgact tctcaatcac agccattggt tcttcaccgt atgcataact
     3481 caaaccaaaa tgtgcagctt gagtcgctgc ttctccggca cttgcccctg ctccgctggc
     3541 agctgtggtt gcatcaccag cagcagaagc accactggca acatctgtta cattgtattc
     3601 ccatgtccaa gggaacacac tgtttccagt gaaccatgat ccttcaccaa accaagtact
     3661 tggtttgaac acttcaccat tcccaatgat ttttttggaa gcttcaacga accaaactaa
     3721 accatagaaa atacgaagtg gcacactcca taagacattg ccgtaacgag aagaatgtcc
     3781 tcgagtcacg ttgcgatcat ctttgatatg gaaaaactca tgcattaaat attggaacat
     3841 ataataacca gaacgaatat caaagaagta tttcaaattc acgatatgtt tcattacgat
     3901 agctaaaaat ccgcttaaat ggatcttatc aaataggtta gctacccccc atttggcacc
     3961 aatagaaacc ataaatcctt gatagtttcc tttaaattta tgcttttctc cgcctttgat
     4021 cgaagccacg atattcgctg cggctgtgtg tcctgtttgt tcagcagctt gaacgatctg
     4081 cggtgtcggt gttcctggga attcttcata aaagactaaa tcaccaatga tatagatgtt
     4141 tttatcttcg tagccttttg cctgcatata ttcattggcg atcaaccgct gtccgcgtgc
     4201 tgattctaat ccaaagtcag cagcgtcaga agttcctttg acaccagctg tccaaatcag
     4261 tgtgtgagtc ggaatgttgc gtccgtcttt caatttgata tgatcagcag ccacttctac
     4321 gattggtgca ttcaataata attccacgtt gtgcttacgc aaatagcgtt cagcttttgc
     4381 agcatcattt cgatccaaca tattcaagat cgtcggcatt gcctctacga ccatcagtgt
     4441 gatttcgttt ggatctaatt tataatcttt ggctaggcga tttttccagt cgatcagttc
     4501 cccaatcatt tcaataccgg taaatccaga accacagaca acgaaagtca gcatggcttt
     4561 acgcagttca tcatctggtt ccaatgcagc ttttgctact gtcttctcaa tatgttcccg
     4621 aatccttaca gaatcttcaa atgaccaaag ggtgaatccg tgtgctttga caccaggtgt
     4681 accaaagtca ttcggctctc cgcccattcc aaggatcagc tgatcaaaag gataggaacc
     4741 taatttggtc ttgacgattt ttcgttcttt atcgataccg attacagtgt ctgtgacaag
     4801 ttgtacattc ttttgacgag caaacaaatg ctgcagatcg tattggattg cttctggttc
     4861 tacacgtccg ccagctactt catgcaactc ggtcatcata gtatggtacg aatgacgatc
     4921 gatcagagta attgctacat cttctttttt cagtttcttt gccaagtatt ttgtagctga
     4981 aacaccggca tatcctgctc ctacaacgac aatattttgc tttgccattg atcatccgct
     5041 ccttatagac tatctatata atgaatatat gataattctt ttagtttgat acttattaca
     5101 caatctccat atattatata gatgttctgt tcttttgtct aactcctttt ggattttttt
     5161 caaattttaa aataaagcgt tttttcacga aatttgctga aagaaaacgt ttgaaatatt
     5221 acatgggtta tttatcaaaa atagttgact tataggctaa aaagattaga atgtataatg
     5281 aatcacaaag aaaaattgtg gactagaaat ggaatggtgg ggtattaaaa tgaagaaaaa
     5341 acgttttgta actggatttg ccgtattagc attttcagct ctagtgttag gcgcttgcgg
     5401 cgcagatgac aacggaagct caaactcttc ttctgaatca tctacagcac aatcttcgac
     5461 tgcaaaaagc acaacagaat catctgcaaa agttgtagct ggcggagatt tgcaagacgg
     5521 cacatataaa ttagaagaaa aaaattattc aaatggttac cgtgcagtct ttgaaatggt
     5581 tgtaaaagac ggcaaaatca ctgaatcaaa atatgataac gtcaatgaaa acggcgaatc
     5641 aaaaacaaaa gatgcagaat acaacaaaaa catggaagca aaatctggca caaacccaga
     5701 aaaatacatt cctgagttga atgaccaatt cttaaaagca caaagcgcaa gcggtgtgga
     5761 agtcgtaact ggagctactc actcatctga aagcttccaa aactacgcac aacaattgat
     5821 ccaagctgct caagctggta acacagatac aatcgaaatc gacaatggtg ctgatttgaa
     5881 agacggcact tacaagcttg aagaaaagaa ctactctaac ggctaccgtg tgcaattcga
     5941 aatgacagtt gctggcggca aagtaactga atcaaacttt gattatatcg acaaagacgg
     6001 taaatcaaaa caagatgata cagaatataa tgaaaatatg aaagcaaaaa gcggtacaga
     6061 accaaaaact tacatcccta ctctgaacga cgaattcgtc aaagcaatgg gcgaagaaga
     6121 tggctcacct gctaatgtag aagttgttac aggtgctaca cacagctcac attcattcat
     6181 catgtacgca caacaattag tgaatgcagc tgaaaaaggc gacactcaaa caatcgaagt
     6241 agataatatc gtaacagaaa aataatcaca taatcatact aataaaagtt ggagggtggg
     6301 acgatagcca gattaggcag tcgtctcacc tttcaaaatg aagagaggaa aaagatgcgg
     6361 aaaaaactac tattttcctt aatcacactg ggcatctctt ttatggtgct aactggctgc
     6421 agcaactcag caaataaaac aaccactgaa agttcagcag ctaccaagat attgaaagat
     6481 ccatattctg atgaacagtt cttgttaggg acttacgttc gtatccgtgt gtatgatgaa
     6541 ggcaaagaat cagcattgaa acctgccttc gatcgagtaa aagaacttgg tgataaaatc
     6601 acgatcaatc aaaaaggttc tgagatcgat gaagtgaatg agcaagctgg gatcaaacca
     6661 gtaaaagttt ctgatgacgt ctacacgtta gtcaagcggg cctacgaata tagccaagat
     6721 tctcaaggcg gattcgatat ggcgattggt gcaatcacac aattatggcg aatcggtttt
     6781 gatgacgcca gaaaaccttc ccaagaagaa atcgatcagg cattgaaatt agtggattat
     6841 cataagatcg aactaaatga caaagaaaaa actgtttatc taaaagaaaa aggaatgatc
     6901 atcgatttag gtgcaatcgc caaaggttac attacggacg aagtagtgaa agtcttgaaa
     6961 aaacaaggag tgaccactgc catcgttgac ttgggcggta atgtctatgt cttaggccac
     7021 agcccaagag gcgaaaacca agactggaca gtcggcatcc aagatccgaa catggctcgc
     7081 ggatctgttt taggttctat taaagaacgg aacaaaacat tagttacctc tggaatttac
     7141 gagcgttatt tagaagtaga tggcaaaaaa taccatcatt tgtttgatcc aaaaacgggt
     7201 tatccgttcg acaatgatat cgcaagtgtc acgatcataa cagataaatc aattgatggt
     7261 gacgggttgt ctacagcagt cttttcaatg ggtgtgaaaa aaggactgga atacgtcgaa
     7321 agcgaattga ataacgggac ggaagcgatc ttcgtgacaa aagacgataa agtatatgtg
     7381 accgatccaa tcaaagacac cttcaaatta tcggaggact cccattacac gatgggtgac
     7441 cgttctgaac taaaataagg agtacgaacc atgacactaa aaatcttttt agaagtagtt
     7501 gaaatacgaa caaaggtggc tagtgtattt ccttttgcgg taggggtatt attctctatc
     7561 gctttttttc atgagataca ttggctaaac acttttttat tttttattgg aatgctagtt
     7621 tttgatttag cgactaccgc tatcaataat tacatggatt atgaaaaggc tcattctgaa
     7681 gtatataagt atgaagaaaa tgtcattggc cgcagaaatc tatctcctgt attgattcga
     7741 aacatgattt tagctatgat tgcatttgtt tttgttatcg gcgttattct aacgatcaat
     7801 acgggttggc ttttactttt gatgggattt atctgttgtt ttattggagt attctataca
     7861 ttcggaccga ttcctttatc tcgaatgcct ttaggggaaa ttttcagtgg ggtgactatg
     7921 ggccttggaa tttttgccat ggtcgtttat gtcaatacat tcggttcaga tgtctatgat
     7981 ctgacagtca acttttcatc tggacaattt gatttggtag gaaatctttg ggctatagca
     8041 gcaatcgtat tagcttcctt acccttggtg tttacgattg ccaatatcat gttggctaat
     8101 aatttgcggg atcttgaccg tgatattgaa aatcatcgct atacactagt tttttatatc
     8161 ggacgtcctg cgggaacagt cttattccag ctattgatgt atgcttgcta tttcgtgatt
     8221 ttaatggggt tgttgagcgg tatctatgaa tggccgatct tggtcacctt cttgaccttg
     8281 ccaattgtga agaagcattt ggatcaattc aagcaaacgt taccccagcc tatcagtttc
     8341 agctattcca tcaaaaatat ggtcttattc aatgccagtt atgtattggg gctattacta
     8401 acaatcatta tgcaagccat ttaacagcat gaagaggttg tgaaaaaact gccctgcatc
     8461 aagtaataag aacagccaaa taaaaatagc tccttatatt tttcgtttgg ttgggcttat
     8521 taccgcagat gcaagctttt gaacaccgtt tatcaggagg ttgtgaaaaa gtcgcttagt
     8581 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct
     8641 taataccgag aaactggctt ttgaacaccg tttatcagag gttgtaaaat cagtgttttg
     8701 tcctgagtat tagagaaaca aatggaaaaa tagttcctca tattttgccg ttttttgagc
     8761 taataccgaa ggacagctga ttgaacacta tttgttcgga aaaagtgact aactatgaca
     8821 caattttttt cacagtccct tttttcactt gatttgattc gtaccagcga acttgacgag
     8881 aactttcttg gtttggtaac tatatggtat tcttttaaca tcctcgtttg cttcttctaa
     8941 tttatttata caaagtcaca cttttttaaa catctgttct tcgatacatt tctatttctt
     9001 ttaaacatcg ataccaatat tttgaactta agagtgcata ccaaatggat tcatttactc
     9061 ttcgtcagtt acgaagataa tcaaaatctc caacttccct acttatttcg ttttgagaat
     9121 tatcgaagca atcttacata aataactaaa aaacaaaaaa ccagaaaatc agtatactga
     9181 ttttctggtt ttacatggtt tgttcaataa aaagctgttg gaactgttaa tattaaatta
     9241 tcgcatcgtt acgaattctt ccgctcctgt tggatggatg gctactgtat tgtcaaagtc
     9301 ttctttagtt gcacccatct tgatagccac ggcaaaacct tgcagcatct catcgacacc
     9361 aacaccaata gcatgtaagc cgacgattct ttcttctttt ccgacacaga tcaatttcat
     9421 ttcgcatttt tggcgataat catttaatgc aaagtacatt ggggtaaagc gtgaacgata
     9481 aactttcaat tcattttcgc catatttttc caatgcttct tcttctgtca aaccaattgt
     9541 tgcaattggt ggatgcgtaa agacgactgt cggaacaagt ttatagtcaa gataagaatc
     9601 tttctttcca ttaaataaac gttctgataa acgtctacca gctgcgattg caacaggagt
     9661 cagatctaat ttaccgatca catcaccaac agcaaaaata tgttcatccg ttgtattttg
     9721 gtatttatct actttgataa accctttttt gtccaattcc acatttgtat tttccagacc
     9781 taatttttcg gtattcggta cacgtccacc ggcaaaaagt acacaatcac cagttaaggt
     9841 ttcaccattt tcaaatagca ccgtatattc gtcattgttt ttagtaattt ctttagcaat
     9901 atgatgtgcg taagttttta ttccagcttc ttggtacatt tggatcaaat tgtccgaaag
     9961 cattttatca aacgttctaa gtggtcgttc ttttcgatat gcccacatga cttcactacc
    10021 taaaccacga aatacacctg aaagttcagc cgcaatgtat ccagcaccta aaacgattgc
    10081 tctcttaggt aactctgtta attcaaaaaa tccatttgaa tcaattgcgt attctccacc
    10141 aggaatggtc attttacttg cacggcctcc agtagcgatc aagattttag gagcacgata
    10201 ggtttcaccg tttacttcga tcgtttgact atcgataaaa gtagcgtaac cttttatagc
    10261 ctcgacatga ttgctatcta atcctctttg ataagcaccg tgtagaaaat caatatattt
    10321 ttctcgtctt tctactagac cggcgaaatc aacatttttt aaatctgctt ggataccgta
    10381 acttgaggca tctcgttcaa ttgtttccaa aatcgtactt gcttgccaca taactttttt
    10441 aggtacacaa ccgacgttga cacatgttcc gcccaattca ttcgcttcaa tcagcaaaac
    10501 ttttgcgcca tgcatccctg ctctgtttgc tgaagcaata ccgccagagc cgccaccaat
    10561 tacgatgtaa tcatatattt gcattttgtt cctccttatt gaagagatca atcaaaacct
    10621 ttatttaacg atcctttttc tacagaaaac aagtgtaaca cttttcttga attttgtatg
    10681 cgaagatgct taaaacaggg atatatcagc tttttcctta tttacgtttt cggatcatat
    10741 ctccaggttt caccggctgt gcgactggca tagttaaaat catcattgga tttggtgctc
    10801 ggtcaatgga ttcgccttct tcattatgca taacttcaac ggtttgactg aaatgactga
    10861 atcctggacc ataaaattcg atttcatctc ctacagaaaa caagtttctc tgacgtatcg
    10921 tagcaatctg tgttgcctca tcataagata atacttcacc gacaaattta tatactggga
    10981 ttttacgacg ttcaccgaat aattgttcgt tttcagttgg tgtattgtag taaaaacctg
    11041 tagctaattc tcgttgtgct actttccata actcgtcgat ccattcttgc ttgcagacat
    11101 agttttcagg atcttccata taactgtcta cagccgcttt atagacattt gacaccgtag
    11161 atacataatg gatcgatttc attcttcctt cgatcttgaa gctgtctacg ccgttttcaa
    11221 tcaattctgg aatatgttcg atcattgaca tatccactgc actcatcgag aattcttctt
    11281 ccacttcgcc tgtatcagtt aaactgtttc gttcttgacc aaaaggcata tcgaacaatc
    11341 catacttcca gcgacaagat tgagaacatc cgccacggtt tgcatcccgc atcgacatat
    11401 ggttggacaa tgtgcatcga ccagaataag agatacacat ggcaccatgg ataaatgctt
    11461 cgatttcggt atccgtattt ttacgaatct ctgccacttc ctccattgaa acttcacgag
    11521 ctaaaacgac acgttccaac ccttcttctt tccagaattc caatgtctca aaattagttg
    11581 ctgaagcttg agtggataaa tggatcggca gacctggggc ttctgtcgca caaatttcga
    11641 tcaaagctgg atcagaaaca atcactgcag aaataccgac atcacgcaaa ctgcggaaaa
    11701 attcccctgc tccatcttca tttccttcat gggtgaccat attagcagca acgtatacct
    11761 tcgcatcatg ttcttttgca aaagcaactc cttcagccat ttcttcataa gtaaaattac
    11821 ctgcacggct tcttaaacca tatgcattcc cgccaatata tactgcatct gctccataat
    11881 ggatcgcggt ttttaacttt tccagtgttc ctgctggcgc taggatttct ggtcgtttca
    11941 attttctttt tactgtcatt tctttcatca tactccttat ttgatagcag aaggatcaat
    12001 ataaaagaat cctgtatcta attcacggtt ttcaggatgc aatttactga tttcgctagt
    12061 cattttttcc gctttttctt gatcccagct accttctaaa atttgttttt ttgcttcggc
    12121 gaatagtcga gtgatgttta cgaatttttc gcctggagta aataatccgt ccaatttcca
    12181 tgtacagtaa tgatgctgat aaagttcatt caattcattc gagagattca gatcattgct
    12241 ggcaaaaata tgtgtgccat gactatcttc ataaatagaa taatgtgtct cttctttttt
    12301 aggttccgag ataaaaagac ctctttcacg atctttttgt tcgtcttgtt tcgtatagtt
    12361 atagtaattc tgtagcagtg gacgtttgga ttgatggatg catgtagcac catacacaag
    12421 ggtttctaca ggaatatcga gtttttcttc catcgccttc atctcttcaa aaggaacttc
    12481 acgagcaagt actgctccaa ctgcgccttt tttcgcccag aaattgattt gacgtgcgct
    12541 cgttactaat gtttcagcat cataaatata cggtatacgc aattcaggat tttttttcat
    12601 cgtataaata atacctgtat caccaactgt gatcacatct acgtttattt cttttaaaaa
    12661 agccaaatat tcaggtacat ttttcatttt ttcaggatga atgatcccat ttacagcaat
    12721 attcgctttt ttaccatact tgtgtgctaa ttctaccaat tgtcgctgtt cttctcgtgt
    12781 aaatgaagca ggcagacgca atccaaattg ctcttcaccg aaatatagtg tatctgcacc
    12841 tgcagcaagc aactgttctg cttgttcaat agtttctact gtggttatga gttcgatcat
    12901 aattttctcc tcctacaact tcataatagt agcatagctc taaaaacttt ccaatacttc
    12961 ttctatttaa agtcggattt ttgaaaatta tttcacaaaa aatcaaaata aagttcgata
    13021 tattctgtcc aaatataaaa agagattgtg acaaaaatct tgtcacaacc tcttaatcaa
    13081 gtcttactat tataaaaatt cagctacaca ggccaagtaa ctatagggaa cctactgttt
    13141 cctttttagg tccgcgaaca ttcaatgtga tcttaccttt tgaagcacct actgttacgt
    13201 ggtcacctaa acgaatttct ccgccaagca aagcctcgct caatcgatct tcaatttctt
    13261 tttgaagtgc gcgacggatt ggacgtgcac catattcagg atcaaatcca gctttcccga
    13321 tcacatcgat tgcagatggt gtgattttca cttgaatatc ttgttctttc aatcgtttga
    13381 taatcgcttt gctcatgatt tggacgattg tatggatttc atctttactc aatgaatgga
    13441 agacgacagt ctcatcgatt cgattcaaga attcaggtcg gaaagctttc tttaactctt
    13501 ctaagatacg tttttgcatg gctttgtgat ctttagtaac atcttggaca ttgaatccga
    13561 catttttttc ttctcgaatt tgtgtcgctc cgatatttga agtcataatc atgattgtat
    13621 ttctaaagtc cacttttcta cctttagaat ctgttaaatg accatcatcc aatacttgca
    13681 gaagtaaatt gaaaacgtct ggatgtgctt tctccacttc atctaagaga atcactgaat
    13741 atggtttttg acgaattttt tctgtcaact gtccaccttc atcataccct acgtatcctg
    13801 gaggagaccc gattaatcgg cttgtactgt atttttccat aaattctgac atatcaacac
    13861 ggatcagggc atcttcactt ccaaacatga cttctgaaag tgctttggcc aattctgttt
    13921 ttccgacacc agttggtcca aggaacatga atgaaccaat tggacgatca ggatctttca
    13981 atccgcttcg tgcacggcgg atcgcacgtg aaactgcttt gacagcctca tcttgaccaa
    14041 cgacacgttc atgcagaagt ccttccagtt ctaacaagcg ttcgctttct tttttctcca
    14101 actgctgtag agggacacca gtccactccg atacaactgt agcaacatct tcttctgtta
    14161 cacgatcaga ataaccttgc tcctgttttg cttcaattgc agaaactttt tgcagctttt
    14221 tacgtagtgc tttttcttgt ctgcgtaatt gagcagcatt ttcaaagtct tgtttttgga
    14281 tcgctgcttc cttttcttgt actagttcat cgatttctag tttgatcaca gttgattttg
    14341 ttaagtgatc ggtttgatcc aaacggactt tcgctgctga ttcatcgatc aaatcaattg
    14401 ctttatcggg tagttgacga gaagtgatat aacgaacaga aagattgact gctgctcgta
    14461 cagcttcatc tgtgatctcc actccatggt gctcttcata tcttgtacgt aatccttgaa
    14521 gaatcacttc tgcttcttca ggagttggct catctacttg aatgcgagca aaacgtcgct
    14581 ctaatgcaga atctttttcg atatattttt gatattcatc tagggtcgtt gctccgatcg
    14641 tttgtaattc tcctcgagct aacgccggtt tcaagatatt cgaagcatca attgctcctt
    14701 ctgctccacc tgcaccaatt aaggtatgca attcatcaat gaataagatt acttgaccat
    14761 cattatagat ttcgtcgatg acctttttca ttcgatcttc aaattcaccg cgatatttcg
    14821 taccagcaac caaagatccc atatcaagca tcatcaggcg cttttcttgc atgtcttctg
    14881 gcacttcacc taaaatgatt ttttgagcta acccttcagc aatggctgtt ttgcctacac
    14941 caggttcacc caccagtaca gggttattct ttgtccgtcg gctaagaatc tggataagcc
    15001 gtttcacttc tctcgaacga ccaacaactg ggtccagctt gttttctttt gctaatttcg
    15061 tcaaatcacg tgctaatgaa tctagcgtag gtgttccttg cggtgtttgc cgtttatctt
    15121 gtacatttac atttctacga cgattcattc cttttccagc agaatcacca gctagcccca
    15181 ttttttgttt cagaacttga cgggcttttg aaaggctgat acctaagttg atcatgattc
    15241 gagtagccat gatactttca tcttttaata agctcatcaa aatatgttct gttccaactt
    15301 tttgagaacc agttttcttc acttcgctgc tagctaaagc aaaaacttgt ttcattcttg
    15361 gtgaatatgg aagaaagact cctttagggt aagaacggat accaccataa ccaatcagat
    15421 gttcaatttc ttcataaatt tccgtttcat tcaaatccat ttcacgtaat acttttccag
    15481 caattccatt ttgctcgacc actaaggcta gtaaaacatg ttctgaacta actgattgat
    15541 gtttcaaccg ttttgcttct tcttgtgcaa tttctaatac tgcttgggct ctttcagtaa
    15601 aaagttcttc catctaatct cacttcccat cttcataact taaacgttcc agaaatgctc
    15661 ggagaatccg agcacgtgta tgatcttcat attcattgaa attcaatgtg ttttttgcaa
    15721 ttgcactcaa catcaagttt ccttcttttt ttgtaattat ttggtcttca taaagttttt
    15781 gaataatagc aaaagcattt ttctcactga ttgtatcatc aaataactga tttatctgct
    15841 caagtaattg ttttttatct gaaatcctta cttttgcaat tcgtatataa ccaccaccac
    15901 cacgtttgct ttctactaga taaccacgtt ggatggtaaa acgggtatta atcacatagt
    15961 tgatttgtga aggaacacag ttaaagaggt ctgccatttc aatccttcga atctcgatca
    16021 tttcactatt ttccagaatt ttctttatat atgattcaat taaatccgag gtattctgat
    16081 gtgccatctt attcattccc tttgactaat attgacctta attataacga atttgataat
    16141 agaaagaaag aagaacgctt tttgttaaat aaatccttta gaatcaacaa aaaaaaatac
    16201 ggaatatact attccgtatc gacgcgccca agaggagtcg aacccctaac cttttgatcc
    16261 gtagtcaaac actctatcca gttgagctat gggcgcatat tcaatttaaa aaaattataa
    16321 atcatttgaa aacaaaaagc aagataaaaa aaatcgggaa gacaggattc gaacctgcga
    16381 ccccttggtc ccaaaccaag tgctctacca agctgagcta cttcccgttt aaaaaataaa
    16441 aaagac
//
LOCUS       DAGCYL010000049        16340 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.050, whole genome shotgun sequence.
ACCESSION   DAGCYL010000049 DAGCYL010000000
VERSION     DAGCYL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.050"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..632)
                     /locus_tag="MB216_001207"
     CDS             complement(<1..632)
                     /locus_tag="MB216_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526053.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNNVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDL"
     gene            complement(811..1530)
                     /locus_tag="MB216_001208"
     CDS             complement(811..1530)
                     /locus_tag="MB216_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4526054.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1661..2812)
                     /locus_tag="MB216_001209"
     CDS             complement(1661..2812)
                     /locus_tag="MB216_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBT4526055.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3029..3769)
                     /locus_tag="MB216_001210"
     CDS             complement(3029..3769)
                     /locus_tag="MB216_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4526056.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3773..4129)
                     /gene="rsfS"
                     /locus_tag="MB216_001211"
     CDS             complement(3773..4129)
                     /gene="rsfS"
                     /locus_tag="MB216_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBT4526057.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(4153..4746)
                     /gene="yqeK"
                     /locus_tag="MB216_001212"
     CDS             complement(4153..4746)
                     /gene="yqeK"
                     /locus_tag="MB216_001212"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBT4526058.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDVEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4730..5380)
                     /locus_tag="MB216_001213"
     CDS             complement(4730..5380)
                     /locus_tag="MB216_001213"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBT4526059.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5390..5701)
                     /gene="yhbY"
                     /locus_tag="MB216_001214"
     CDS             complement(5390..5701)
                     /gene="yhbY"
                     /locus_tag="MB216_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBT4526060.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5717..6826)
                     /gene="yqeH"
                     /locus_tag="MB216_001215"
     CDS             complement(5717..6826)
                     /gene="yqeH"
                     /locus_tag="MB216_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBT4526061.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6823..7350)
                     /locus_tag="MB216_001216"
     CDS             complement(6823..7350)
                     /locus_tag="MB216_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBT4526062.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7577..8362)
                     /locus_tag="MB216_001217"
     CDS             complement(7577..8362)
                     /locus_tag="MB216_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBT4526063.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(8355..9224)
                     /locus_tag="MB216_001218"
     CDS             complement(8355..9224)
                     /locus_tag="MB216_001218"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBT4526064.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9225..10604)
                     /locus_tag="MB216_001219"
     CDS             complement(9225..10604)
                     /locus_tag="MB216_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBT4526065.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10612..11031)
                     /gene="fabZ"
                     /locus_tag="MB216_001220"
     CDS             complement(10612..11031)
                     /gene="fabZ"
                     /locus_tag="MB216_001220"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBT4526066.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11056..11532)
                     /gene="accB"
                     /locus_tag="MB216_001221"
     CDS             complement(11056..11532)
                     /gene="accB"
                     /locus_tag="MB216_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBT4526067.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11535..12770)
                     /gene="fabF"
                     /locus_tag="MB216_001222"
     CDS             complement(11535..12770)
                     /gene="fabF"
                     /locus_tag="MB216_001222"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBT4526068.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIIGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKRDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12789..13526)
                     /gene="fabG"
                     /locus_tag="MB216_001223"
     CDS             complement(12789..13526)
                     /gene="fabG"
                     /locus_tag="MB216_001223"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBT4526069.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13538..14455)
                     /gene="fabD"
                     /locus_tag="MB216_001224"
     CDS             complement(13538..14455)
                     /gene="fabD"
                     /locus_tag="MB216_001224"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBT4526070.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14562..14786)
                     /locus_tag="MB216_001225"
     CDS             complement(14562..14786)
                     /locus_tag="MB216_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBT4526071.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14839..15801)
                     /locus_tag="MB216_001226"
     CDS             complement(14839..15801)
                     /locus_tag="MB216_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBT4526072.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15811..16251)
                     /locus_tag="MB216_001227"
     CDS             complement(15811..16251)
                     /locus_tag="MB216_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4526073.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4312 a         3728 c         2794 g         5506 t
ORIGIN      
        1 tccaaatctg tttcaggata ttttaatttg acaagcgaaa taatcatttt cagttcttcc
       61 atgttttctg gatattctgg gtcaaatacc ttgctatgac ggccaatccc gccattgtcg
      121 atcaatcggt aaattcgatt ccattcaatg aattggtcaa ttgattggaa attgctgatt
      181 ggatagccgc cacaatccaa aatctctgat acacgaaatg ctaggtatgc actatcgatc
      241 acattatttt ttttcgtgtc tatcttctca tttctttcga taagaatttt ttcaggacga
      301 tgcttcaagc tccactctaa tagtagaaag aaagcctcca tccgatactc ttcttcaaat
      361 aaccgaaagg ctgaatcaga acgacgaaaa agcagcggaa tcagataatt ttgtctataa
      421 atcagcattg gtgcgcggtc caccatacac tctaaataac ggttcagata ccctgtaccg
      481 acatagtttc ctaatcgata gccttcaacg ccgaatcttg cgtatttttg cgctaatttt
      541 gaacaatcag ttacgttcac agctaactct tctactttgc ggtaaccgct taagatacgt
      601 tcaaaaggtt ctaaagtgat ttttgcttcc atattcttcc ccgtccatct tccacatact
      661 tcatatcatt tcacatgata cttatgctct catcatacca caggttgaat tagaaaagaa
      721 aataaatcac acaaaaaagc aagacaatct gttagtgaga ttgtcttgct tttcgaaaaa
      781 gactgaatac ttttcgttaa aactggttgc ttagtcttct aatgccacat tatggaacac
      841 atgctgtaca tcttctagat cttccagtgc atcaatcatt ttttcaaatg tttcttgatc
      901 ttctggagat agtgttacct cgttttgagg gatcatttgg atctcagcga ttgaaaaatc
      961 agtgattcct ttttctttta atgcttcttg tatactatgg aaatccgcta cttcaccgta
     1021 aacgatgatt tgtccttctt cttccattac atcgcgaaca tcgatgtctt tttccattag
     1081 gtattcaagg atctcgtcag cgtcttctcc tgcaaaagca aagattgctg tattgtcaaa
     1141 catgtaagaa accgctccgg aaacacccat gttcccgcca tttttcccaa atgctgcccg
     1201 tacgtctgct gctgttcgat tcacattgtt cgtcaatgta tcaacaatca ccattgagcc
     1261 gcttggtcca aatccttcat agcgcaattc agagtatgtt tcatctcctg caccttttgc
     1321 tttttcgatt gctcggtcaa taatatgttt tggtacatta taagttttag cgcgatcaat
     1381 gacgaaacgt agtttttggt ttgcatgcgg atcaggatca cctgattttg ctgccgcgta
     1441 gatttcaatc ccaaattttg cataaatccg gctattatta gcatctttag cagttttctt
     1501 tgctacgata tttgcccatt tacgtcccat attttcactt cgctttctga tttgatagtt
     1561 tcataacgca aaaacgtcct gttccattat acttgtgaat cacgattctg tgaagctttt
     1621 tttctttctt ccttcattct tctcattttt agtcaattat ctatactcgg attgggattt
     1681 gcccgaaatt ctgttccgga atattgcctc ccaattgata gatttgatca cttctaacta
     1741 acagttccgc ttcttgtgcc tctttttttc cgattcggga ataaatagaa atctcttttt
     1801 tttcttttaa gaatcttctt ccaacatcgg taaatccaag tagtcgaaga taatttttat
     1861 tttgttggac aggaatctct tgctttttga tattcagcaa gacataacaa gataatcgtt
     1921 ggatccgtgt ccatgtatat ctttttgttt ttacctttga cacaaaatcc tcaaaactat
     1981 cagcagttgc agctgctgtt ttcatgcgcg attctattcc ttcggtcatc tgatagatat
     2041 ctgtcagttc cagcaaagta gaagataaca accgataacg caaatacggc caataagatt
     2101 cccaagtctg cagcgaataa gttgaaagat cttcttctgt ctgaggcggc acaagtccag
     2161 ctattctttt tccttcttct aacgcatggc gaatggctgt tgcactggct atcctctgat
     2221 ctgtgatgat tttttcatga tagcctgcac ttttacgtcg gatcggatgt aattccatag
     2281 ggtggacata cttggcattt tcctttgcat aacttaatcc caagatatga tttggcgaag
     2341 aaaaatcaag cgtaatatcg ggatacagac tgcgaaaaac ctcagtcatt ttttgcggat
     2401 aactatacgt ttgatcagac agttcttgaa atgcctcttc tatcctatct tgattctctt
     2461 ggacaaagcg tccgaaactg ctgtaatcaa acgaagtctc ttcttctgtt ccaaaacaca
     2521 aactgtcaca gtccagtgtt tgcaagattt ttactgcacc tttagcaaaa tagtcagctg
     2581 actgaaccgc ccattcgacg ggaagctcca ctactaaatc cacaccattt tgtaaagctt
     2641 cattcgcgcg atgccattta tcgatgatag ccggttcgcc tcgttgcaaa aaattcccgc
     2701 tcattacagc aatgacgacc tctgctcctg ttgtttttcg tgccatttcc acatgatagc
     2761 gatgtccatt gtgaaaagga ttgtactcta cgataatccc gcagcttttc atgcgcatcc
     2821 tccttcatag ttatcgataa aaaagaaata gagacaagta aaaaaaggca aaaatcggaa
     2881 aaaatacccg aactatataa actattctcg caaacacaat acttctgcga cgaaaagctg
     2941 cactgcaaga gcaatgccta tatcgttcgg atatataatt tatgttgaag atttattctt
     3001 ttcttgttaa ctcgagcagg caaaaccgct agtctttgta tgctacaaag aaccagcgct
     3061 ttgatttctc tgatgctgct tcgtctgtaa aatcagaaaa tgcttcgact ttcgaaaagc
     3121 cgctgttttc cagcattctt aaataacttt ccaatggata ggtacgctct tgatgtagtt
     3181 catcttcacg aataaatcga ccagaccctt cttgttcttc aacgaaaaag gtaagaaaat
     3241 gttcgatgct gtgtttctcg cttcctggat aactatccca caaaaaagca aaattatccc
     3301 cttgataatg atagctatat tctggaaaaa tttcatctac ttgataggtg gagtgtacgt
     3361 caaataaaaa tcggccattt tcttctaacg cttgatagac ttcatcaaac acttgctgta
     3421 cctcttgtct atttttcata tagcacagcg aatcagaaaa acaggtaatt gcctgatatt
     3481 gtccgatttc tgataaatcg agcatattgc cttccacaaa ttggatcgat gcgtcttctt
     3541 catatgctcg ctggctggca atcatcaaca tctcttctga aaggtctaac cctgtcactt
     3601 tatatccatc ttttgcaaag gcaactgcta atgctccagt accgcatgcc agttccaaaa
     3661 tattttttgt gtcttcttgt aaatgacgtt tcgaaaaagc tagccattgc tgataaagtg
     3721 tttcgtccat cacttcatcg taaatgaaag caaatgtttc ataagccata tgttaatcca
     3781 accattcact aagatcgact agcggagcat ctgaccatag tttttccaga ttatagaaac
     3841 ttcgttctgg tgcatggaaa acatgtacga tcacatctcc aagatcgatc aatacccatt
     3901 ttccgccttc ttttccttca atacgtttta cttcatattt gttttcttct tctttatcaa
     3961 tgatttcttc tgtaattgcg ttgatttgac gttcgctatt tgctgaacag atcatgaaat
     4021 aatctgccaa caaggaaaca tttcgaatgt ctaaagcgat aatgtcttct gcccgttttg
     4081 aatctgctgc ttcaaccgca atttttaatt gttctttact atctatgatg atttcctcct
     4141 aatatgagca gcttattttt ttgctaccca gtaattatat gtttcgattg tttttggata
     4201 aatctgttgt tcttgcgcaa gcaaatgggc caatgtatgt tttgtttcaa atgctacagc
     4261 ttcgtctaga tcagcccatg caatggctcg tgcatcttgt accccaggaa aatctcgacc
     4321 tggttcaata tagtctgcta cataaatgat tttgtcaagg agactcatct tagcagcccc
     4381 agtcgtatgc acccggattg catgaagaat ttctacatcg gtgattccta attcccgctc
     4441 tacaaacgaa gcgccaacaa gaccatgcca gattgcatta ttataattca ataactctgg
     4501 atcaaaaccg tctcttacga ttaccatttt gaactcctca tctggtcgtt cttttgcata
     4561 atcatgtgtc aatgcagcaa tactagcttt ttcaggagaa gcaccgtatt ttttggcaag
     4621 agccacggct gtttcttcta ctccaagcac atgcttgaag cgttgttcgc tcattctcat
     4681 ctgtattttt tgcatcaggt tttctctaga ataagcagta tattcattac tatagtccat
     4741 attcatacaa cccttcattt tgaatataat caattacttt atctggaaca aggtagcgga
     4801 tagaacagcc ttctttgatc ttttgacgta ttttcgtgga actgatgtcg atctcaggta
     4861 catctaccca aattacagga tatggagtat ccgttgtgta tcctggtcga cgtataccca
     4921 caaagttgac catagaagtc agctcatcga ttttatacca ttttggcaaa tattctacca
     4981 tatccccacc gataataaag taataatctg tatccggatt gttttgcgtc aattccttca
     5041 tagtatcata ggtgtaactt ttcccaccac gagctaattc aatcgtttca atctgcaaaa
     5101 aaggattgtc ttccacagct aattccaaca tgttgagtcg atgttttgca tcaatcgtct
     5161 gtttctcatc aacatgaggt ggctgataag aaggcatcaa aaatacgcgg tcaagaccta
     5221 aattccggcc agcttgttca gccatcacta aatgtgcaag gtgaacggga ttgaatgttc
     5281 cgcccaagat ccctacttgc tttttcttct ctaagaattg aggcatttct tcaggaaata
     5341 attgtgaacg aacaaatgtc ttagcttgcg tattcatcat ggtcactcct tatattgctt
     5401 ttacttcttt tgatagcctt tgatactttt cttttgtgga aggtttgtat aatacaataa
     5461 cacgtccaat gatttggacg atttcgcaat ctatttcttt ttctaatact tgagctgctt
     5521 cttctgcaat ctcatctgta ttttgaagta acgatacttt gatcagttct cttttttcta
     5581 aagcttcttc aatttgaacg atcattgctg aattgatccc accttttccg atttgaaaaa
     5641 tcggctgaag atgatgcgct ttgctacgta aaaaacgctt ttgttttcct cttaaactca
     5701 taattttcct tcttccttaa atcaatgctt tgcgtttcaa tacatctaca ccctttggcg
     5761 cccagcccgc aattactact ggcgctgtta cagtgatcca gcctagaccg gcaaaaacga
     5821 tgtctgtttt ttctttcaca gaaaactcaa aacgaacaag ctccggaaat gcctcgactt
     5881 catcaggacg aggaggctgt aataatccac ctacatgttt ttgataaaat tcgtctgctt
     5941 tttccagttt cgttcgatga atatttacat catttgacac ataagcaata aatgaagcac
     6001 gattgccgct gataaaatca aatcttgcaa gtccgcccaa gaaaagtgtt tgcccttcat
     6061 tcagctgata aactttaggt ttgatttctt ttaacggtgc aatgatccgc aaatcttttt
     6121 tacctaagta gtgcgccatt tgatggcgat ggataattcc cggtgtatca atcaaaaaat
     6181 gcccgtcatc taaaggaatc tcgattttat ccaaggtcgt tcctggaaac tgcgaagttg
     6241 tgattacatc ttgtactccc gctgtatttt tgatgatttg attgataagc gtcgattttc
     6301 cgacatttgt tactccgacg atatagacat cccgtccatc gcggtattct tcgatttttt
     6361 ctaatagttc cgtcatctca tgagatttct tagcactagt taataataca tctactggac
     6421 gaattcctgc ttcgtaagca cgttccttca tccattgtgt taatttagtt tttttcaatg
     6481 atttaggtaa tatatccact ttgttcccaa ctaaaagtac tggattgtca ccgacaaaac
     6541 ggtgtagtcc tgggattagt gaaccattga agtcaaaaat atccaccaca ttgacgatca
     6601 atgcatcttt ctgccctaat tcattcagta agcgcaaaaa gtcgtcatcc gtcaatgaga
     6661 catcttggat ctcattataa tggcgtaatc tgaaacaacg ctgacaatag acttccccgg
     6721 aagctaaacc tttttccaaa gccgtttgcg gcgtgtatcc taattcaccg ggtttgtttg
     6781 tttgtataac ggcaccacag ccgatacatt gtaattcttc tgtcattcga ttccacctcg
     6841 ccagcccata tcagggtgtt ttgctaatag ataggccata attctttttt cccaaaaacg
     6901 attaaattgt gtcttccaac tatctgttgc gataatgggt tttacaagga tgctacgtat
     6961 ccctgcacga ttggctccac gtatatcagt cataatctga tcgcctacca ttactatctc
     7021 atcatctgct aaatccaatt gtttctttgc tatgttgatc cctcttgcta aaggtttcat
     7081 tgctcgtgca acatacaaaa gatcaaattt ttcaatcgct cgcgcgacac gttttgaatt
     7141 attgtttgaa acaacaacaa cagggattcc tgcatttttc atttctaaaa tccaagtaag
     7201 caactcttct gttccatctg gattattcca tgcaatcaac gtattgtcta aatcggtcaa
     7261 tactgctttg atgcctagat tctttaattg ggcaggtgta atctgataga tggcttctac
     7321 catccatgtt ggtttgtaat ttgaaaacat gctgtctcct catctgcaaa aaatgaagga
     7381 gcacataaag tactcctcaa ccccgtatta tactgcatat cagcaaaaaa aactacctct
     7441 atactcgttc gtctttcaga aaaagcattt ctttacaaaa tgaccaaaaa taaagattga
     7501 tcacagaaaa atttagaaac aaaaaacaac taagcgagca agaccattct ttactctttg
     7561 cttagttgtt aaacacctaa tattggcgga acctcttgta tcttttttcc agtaattcat
     7621 gctggtcgaa tcgaacaagt tccgtcaatc tttcaatcaa cgcttttttc ataatgtgtg
     7681 cgactttata tcgtggcaac tgctcgccgt tgaacgtttc aggaataact cgatcaacga
     7741 ttcctaactc gcgtagttct ggcgcagtaa ttttcatcaa ctcagctgct tctgcggcgc
     7801 gactaccgtc tttccacaaa atcgacgcaa atccttctgg agaaagcaca gcataaatcg
     7861 tttcttccag catccagact tcatccgcta aacctagagc aagggcaccg ccacttccac
     7921 cttcaccaat gatgatggcg atgatcggta cctttaaatc agacatttcg agcagatttc
     7981 tggcaatagc ctctccttcg ccgcgctctt ctgctcctac accgcaatat gcacctgctg
     8041 tattgatcaa tgtaatcacc ggtctattga acttttccgc ttgtttcatc agtcgtaaag
     8101 cttttcgata gccttcagga tggggagaac caaaatttct gcggatattt tcctgcagat
     8161 cccgtccttt ttgaataccg atcactgtta ccggtttgcc atctaatcga gcgattccac
     8221 caacaatcgc ttcgtcatct gcaaacaaac gatcaccatg gcattcctcg aaagaatcga
     8281 agatctcttc aaaaaaatcc aagcttgtcc atcgttcttt agctcttgct aactgaacga
     8341 catcatgtgc tgtcttatcc acgatctatc catcctttct gtgtatgcaa ttcaagaagc
     8401 gtatggattt tctgcttcaa ttccatgcgg ggaacaatct gatcgacaaa accatgagat
     8461 aataagaatt cagccttttg gaaatcttct ggaagttcct gcttgatcgt ctgttcgatg
     8521 acgcgtcttc cagcaaaacc gatcaagctt tggggttctg ctagaatgat atcgccttcc
     8581 attgcaaaac tggcagttac tcctccggtc gtcgggtccg ttaaaacagt tagataaaaa
     8641 agaccttcat tgctatgccg tttgacagca gcagagattt ttgccatctg cattagagaa
     8701 aagattcctt cttgcatccg tgcgccacct gaagctgtga acaagacgac tggaagactt
     8761 tcctttgttg ctcgttcgaa taatctggtg atcttttctc cgacaatcgt tcccatactt
     8821 cccatgataa aattaggatc catcactcca ataatgaagg gaatcccttg aatggttgct
     8881 ttaccagtga ggactgcttc atgaagtcct gttttatctt gcattttttt aattttttcc
     8941 gtatattcgg gaaatgctag cggatctttg gttacaagat ccgtatccca ttcttcaaaa
     9001 cttttttcat caataatcaa agctagacgc tgccatgcac cgattcgaaa agcgtatccg
     9061 caatgagggc atactttttc ttcaccgatg tctttcgtat aaaggatatg tttgcaattt
     9121 ggacactttg cccacatgtt atcaggaaca gatggcgagt tagcaggttt gctctctcta
     9181 ttaggattga tccgaatata tttctttttc ttaaacagag ccatctatgc gcctcctatc
     9241 tccgtttctg gtgtccaatt cggtaaaaat tcttcttgta aaaatgctgt gctgtaatca
     9301 cctgctatga ctgcagggtg actaatcaaa tccatctgaa attctgcgtt cgtgatgatt
     9361 ccttctgtga taaattcact taaggctcgc tgcatcttca ttaatgcttc aaaacgcgta
     9421 tttccatgga cgattatttt tgcaatcatt gaatcataat atggcgggat cgtatatccc
     9481 gggtaaactg cactgtctac ccgcagtccc atcccacctg ctggaagcag taagttttgg
     9541 atcttccctg gagaaggagc aaaatggaaa gcaggatttt ctgcattaat ccggcattcg
     9601 attgcgtgtc cttggaatac tacttcttct tgacacacat tcaaaggctc accggctgct
     9661 atctccacct gtttttttac taaatcgatt cctgtgacca tttctgttac tggatgttcc
     9721 acttggattc gagtattcat ttccatgaaa taaaaatcgc cttcttcatc catcagaaac
     9781 tcgatcgttc cggcattttc ataattgact gctttagctg ctcgaacagc agcgtctcct
     9841 agtgcttctc tttttgtttg tgagatcact acagaaggag attcttccag tactttttga
     9901 ttattacgtt gcaaagaaca atctcgttct cctaaatgaa tgacattgcc aaaatgatca
     9961 cctaaaatct gaacttctat atggcgagca ggataaatga ttttttccaa atacatatca
    10021 tcatttccaa atgccgctgc tgcttcttgc tgagctgaag aaaaatgctg tgggagctct
    10081 tcttttgatt gaactttccg aatgcctttc ccgccaccac ctgcagctgc tttcagcatc
    10141 actggataac caattctgtc tgctatttcc aatgcttctt ctactgtaga taatacacca
    10201 gtacttcctg gaatgactgg gacatttgct tcttgcatca attgacgcgc attgatctta
    10261 tttcccattg catcgatcgt tgcactttta ggaccaataa atgtgatatt gcactcttca
    10321 cacatggagg caaatcggct attttcagat aaaaaaccaa atcctggatg aattgcttcg
    10381 gcttttgtca cgattgcagc gctcagtact tcctgaacat tcagatatga atcaacggct
    10441 tttgccgggc caatgcagat tgcttcatct gctaactgtg tatgaagtgc ttcggcatct
    10501 gcttctgaat atatcgctac ggtttggatc cctagttcgc ggcatgcacg aatgatacgt
    10561 acagcgattt caccgcggtt agctatcaat actttagaaa acatatttca cctatccaat
    10621 cataaaggtt aattccgctt cggctacttt tttaccgtct actttagcga ttcctttgcc
    10681 tattccggct gaagctttca ctttcaatat ctcaacttca aggtataagg tatctcccgg
    10741 tgtaactttc ttacggaatt ttgctttgtc caatccgccg aaataagccg tctttccctt
    10801 gaattgttca agagaaagta atgcaactgc tcctgcttga gccatcgctt caacaattaa
    10861 tacccctggc ataacaggtt cttctggaaa atgtccttga aaaaagggct cattgatcgt
    10921 tacatttttc ttagcaacga tccgttctcc ttcaaccatt tcttccactc gatcaatcaa
    10981 aagcattgga taacgatgtg gaatgatttc ttttatttct tgtatgttca ttgatttccc
    11041 tctttcctgc tttgcttatt tttgctgaat cctaaagagt ggctgtccat attcgacaac
    11101 tgactcgttt tctacaagga tttctgtgat cactccgtcg gcctcactag ttatctcatt
    11161 catcagtttc atcgcttcaa taatgcagat aacttctcct ttggcaactt gatcacctac
    11221 ttttttgaaa gctggtttgt cgggcgccgg ttgcagataa acgattccca cgattggcga
    11281 aacaacctcg attccatcaa atactgccga ttctttttct ttcattgttt ctggagcagt
    11341 tgttgcttgt tcaggtatga cattgactgg ctccgaaact ggcaacgttt cgtttgtttg
    11401 aaccatattc atgcctcgac ttgttttatt tttattcata tacaattcaa attgaccttc
    11461 acgcaaatcg aattccgtta gcgaggattg atcaaattga ctgactaatt ctctgatttc
    11521 attgatatcc aaaattattc ctcccagcgt ttcatgcaca gaaccgcatt atgtccgcca
    11581 aaccctaatg aattactcat tgcataagta aaacttgcag gtttgctttc gtttttgaca
    11641 actgtcaagt caatctcagg atctcgtttt tctacattga tcgtaggtgg gataaattga
    11701 tgttctaatg ccagcaaagt tgccaaggct tcgatcccgc cggctgctcc caataaatgt
    11761 ccagtcatcg atttcgtgct gctgacgtaa gtatctctga aattttcacc taatgcgatt
    11821 tggattgctt ttgcttctgc ctggtcattg gctggtgtac ttgttccgtg agcattgata
    11881 tatccgactt gttccggctg tacaccagct tcttccatcg caagcttcat tgctttagac
    11941 gcaccagaac catctggagt aggagctgtc atatggtaag cgtcacagtt tgcaccgtat
    12001 ccaatgattt ctccatagat tttggcaccg cgagcttgtg catgttctaa agattcaaga
    12061 actagaacac ctgctccttc acccatcaca aaaccattcc gattttcgtc aaacggaatc
    12121 gaaccacgat taggatcagt tgattctgag agtgctgtta aagctgcaaa tccagcaatc
    12181 cccatctcac aaatcgttgc ttctgttcct ccagcaagaa tgacatcaga atagccatgt
    12241 ttgatgttac ggaatgcttc cccaattgaa ttcgttccgc tagcacaagc tgttacgata
    12301 gctgtacaaa taccttttgc acctacgcgc aaagcaatat ttccagctgc catgttagca
    12361 atcgccattg gtacgaacat tggagaaact cgttttgcac ctttttcatg tagacgaata
    12421 acttggtttt cgattgttgg aagaccaccg atcccagatc cgatgatgac gccaaaccgg
    12481 tcttgattca ttttttcttt atctaaaccg ctcatctcca ttgcttctaa agcagcgtag
    12541 attccataga tcgaaaacat atccattcgt ttgctatctt ttttaataaa atatttatcg
    12601 taagggaaat ctttgacttc acccgctaag gtaatccctg tttctgaagc atcaaattta
    12661 gtgatcggtg cgatgccatt cgtcccctct tttaagctat tcaaaaagct gtctgcgtca
    12721 tttccaatag gtgaagtgac tccatagcca gtaattacca ctcgattcat ttgtttgctc
    12781 tccttttctt atccgtgcat gaccaatccg ccatcgacat tgatgacttg tccggtgata
    12841 tatggatttt gtgctaagaa aactgctgtt tgtgcaatat cttctacttg tccgaaacgc
    12901 tgcatcggaa tttgcttttc cgcttgttcc ttgactttgt ctgctaaaac ttccgtcata
    12961 tcagttgtga tgaatcctgg cgcaatggca ttacatgtaa tgcctctagt tgcagcttct
    13021 cgtgccacag attttgttaa tcctactacg ccagctttac tagctgcata atttgcctga
    13081 ccaatatttc caatcaaccc agatacacta gagagattaa tgatggcccc ttctcgctgc
    13141 ttcatcattt ttttcaatac gtgctgcgtc atattgaagg ttccaatcaa attgatatca
    13201 agacattttt tgaaatcttc tgcatccatt cgcatcacaa gtttgtcatt tgtgattccg
    13261 gcattgttta ctaacacatg gatcgatcca agcttttctt ctgcttcttt gatcatttgt
    13321 cccgctttct cataatcaga gatgtcaccg gaaactccta cgcattttac accgaatgct
    13381 tcgatttctt ctatcttttc ttttggaatc tctccgcgac cattcaaaat gatatttgct
    13441 ccagcttttg caaaagctaa ggccatcgct ttgccgattc ctcttgtact acctgtgata
    13501 aatacatttt ttccttttac atccataacg ttcctcctta tagtattgct attgtttcat
    13561 caaatgtctg cttattctca acacgcagaa ctggtattgt tttatcgatt tttttcataa
    13621 atccgcttaa tacttttccg gggccgactt cgatgacttg ttcgatgccg attgttttta
    13681 acttatggat actctcatag aaacgaacag gttgcatgac ttgctgttca agtagtcccg
    13741 cgattgtttc tttcttcatt atttccgttg ttgtgttgct tatgatagga aaagaaggtt
    13801 cagagaattg aatctgactt aggtccttcg ccagtttttt tgctgccggt tctaaaatag
    13861 ctgtatgaaa aggcccgctg acatttagag ggatcattct tttgaaccct ttttctttta
    13921 atagtaaaac agcttcgtct actgcttttt cttcgccacc aatcacgatc tgctgaggtg
    13981 tattataatt tgcaggagat acgatgccgt acttgctagc ttcatgacag ctttcctcga
    14041 tcgtttcaat cggcgcattc atcactgcaa ccattttccc gcttccagct ggggcagctt
    14101 ctgtcatata agcgcctctt tttgctacta acgcaactgc ttcagtgaag gatagcgcat
    14161 tactagctac taatgccgaa tattctccta aactcagtcc taaagctgca tctgggatga
    14221 ttccatgctc cttcaataag cgataatacg cgatgctgac tgttagaata gctggctgtg
    14281 tatattgcgt ctgatccaaa cgttcatttt ctgtaaagca aagttcagcc atatcgtagc
    14341 cgagaatctc acttgcttca tcaaatgtct gctttacaac ggcttcttca tataaatctt
    14401 ttcccatacc ttgatattgt gcaccttggc cactaaataa gaatgctgtt ttcatgtttt
    14461 tctccaatat atccagaata tatgtttgat tcttcctatt caaaaaggcc gagacattcc
    14521 tatttcagga aatgtcccag cccccaacct aattttaatg attatgcagt ttgtttttct
    14581 acgtaagtca ctaaatcttg aaccgttttg attccgtctt ctgtttcgat tttcacgtca
    14641 aattcatctt caatttcatt gatgatttgg aataaatcca agctgtcagc ttctaagtct
    14701 tcttggatgt cagtagtcaa tttgatttct tcagtttctt ttcctaattc ttctgcgatg
    14761 atttcttgaa ttttttcgaa taccataata atatattcct ccgatattgt ttgcttttta
    14821 tttttataat ttaacagtct acaactccat gagaatactt ccccatgtta aacctccgcc
    14881 atagcctgta aatattactt tctgctgttt tcccaaggtg atctttccac tagaaactgc
    14941 ttcatccaat aagataggga tgcttgcagc agatgtattc ccatatttat ccatattggt
    15001 caggaacttt tcttgcggca tctttacttt tcgggcaatt ttctcaataa ttctgaggtt
    15061 tgcctgatgc aaaagcagat aatcaactgg tttgtctttc atgacttctt ggatattgtt
    15121 tgcaacgtct cgtacggcaa aatcaaaaat atctctgcca gtcatttgca aataatacga
    15181 accttctgaa tcttctgaat agaacggatt tttattttgc tggtagccag cggtcaatga
    15241 gctacttctg gtaccgtcag ctgctaattt ttctttcagc aattttttct gtggattcgc
    15301 ttctaagaga atcccacctg ctccatcgcc aaacaacaca gcagtcgtac gatcagacca
    15361 atcgattagc ttagatagtg tttcaccacc aatcactagt cccttagttg agccgcagcg
    15421 gatcattttt tcagccatgc ttaacgcata aacaaatcct gagcacgcag cactgatatc
    15481 aaaagcgata gcatgagttg ctcccaattt cccttgtact tgtgctgcta cagatggcgt
    15541 cgtgtaatcg ggcgacattg ttgctactaa aataaaatcc aaatctttag ggtttagatt
    15601 tttctgtttt atcaattttt ccgccactaa aatgcataaa tcagaagtct cctgatttgt
    15661 tacacatctt cgttcacgaa tacccgtcct tgacgaaatc cattcatcag aggtatccat
    15721 catcttggaa agatcattat tagtaataat cttttcagga aggacgctgg ctgttgcagt
    15781 aatcattcca aattgttcca ttaaatatcc tcacttatag tcttgcaaga agtcgtgcaa
    15841 gtttttcagt gcatccagca aggcatgctg ttcctccgta gccattccgt ctaagatgtt
    15901 cttgaccatt tcacgatgaa aatgctgatg gacccgaaat agtaactttc cttttttcgt
    15961 tagtcccaac tttacaacgc ggcggtcatc ttcactgcgg atacgttcga catagccttt
    16021 ttttactaac ttgttaatag cagtggtcaa ggtcccaaca gtaattgata gttctttggc
    16081 tacttctgaa gttgtttttt tcttgtacat cccgattgcc tcgatcgtgt gcatctctgt
    16141 gattgacaga tctttgaatt gagatttttt cagctcagac tcttcgattg tcaaaatatc
    16201 attgaagaca ctaacaagat aatcatttac tgtttttaaa tctggttcca tgttccaccg
    16261 tccactaatt agtttgatta tcaaatcttt tgatgttcaa actatatctt aacttttttc
    16321 aaaatgcaac cttaaaagat
//
LOCUS       DAGCYL010000050        16322 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.051, whole genome shotgun sequence.
ACCESSION   DAGCYL010000050 DAGCYL010000000
VERSION     DAGCYL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.051"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(103..501)
                     /locus_tag="MB216_001228"
     CDS             complement(103..501)
                     /locus_tag="MB216_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBT4526074.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(498..1271)
                     /locus_tag="MB216_001229"
     CDS             complement(498..1271)
                     /locus_tag="MB216_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBT4526075.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1268..1951)
                     /locus_tag="MB216_001230"
     CDS             complement(1268..1951)
                     /locus_tag="MB216_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBT4526076.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2118..3005)
                     /locus_tag="MB216_001231"
     CDS             complement(2118..3005)
                     /locus_tag="MB216_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4526077.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3002..3871)
                     /locus_tag="MB216_001232"
     CDS             complement(3002..3871)
                     /locus_tag="MB216_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4526078.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4008..5294)
                     /locus_tag="MB216_001233"
     CDS             complement(4008..5294)
                     /locus_tag="MB216_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4526079.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5567..6145
                     /locus_tag="MB216_001234"
     CDS             5567..6145
                     /locus_tag="MB216_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBT4526080.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6145..7074
                     /locus_tag="MB216_001235"
     CDS             6145..7074
                     /locus_tag="MB216_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4526081.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7071..8807
                     /locus_tag="MB216_001236"
     CDS             7071..8807
                     /locus_tag="MB216_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBT4526082.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8870..9706)
                     /gene="pheA"
                     /locus_tag="MB216_001237"
     CDS             complement(8870..9706)
                     /gene="pheA"
                     /locus_tag="MB216_001237"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBT4526083.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9703..10212)
                     /locus_tag="MB216_001238"
     CDS             complement(9703..10212)
                     /locus_tag="MB216_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBT4526084.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10205..11500)
                     /gene="aroA"
                     /locus_tag="MB216_001239"
     CDS             complement(10205..11500)
                     /gene="aroA"
                     /locus_tag="MB216_001239"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBT4526085.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11513..12604)
                     /locus_tag="MB216_001240"
     CDS             complement(11513..12604)
                     /locus_tag="MB216_001240"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBT4526086.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12621..13787)
                     /gene="aroC"
                     /locus_tag="MB216_001241"
     CDS             complement(12621..13787)
                     /gene="aroC"
                     /locus_tag="MB216_001241"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBT4526087.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13789..14853)
                     /gene="aroB"
                     /locus_tag="MB216_001242"
     CDS             complement(13789..14853)
                     /gene="aroB"
                     /locus_tag="MB216_001242"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBT4526088.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14886..15908)
                     /gene="aroF"
                     /locus_tag="MB216_001243"
     CDS             complement(14886..15908)
                     /gene="aroF"
                     /locus_tag="MB216_001243"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBT4526089.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4702 a         3617 c         2900 g         5103 t
ORIGIN      
        1 tttactattt cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca
       61 tccgccttct tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc
      121 tctatcgtta ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat
      181 cttagtgata ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat
      241 atcgatgttg atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac
      301 taatgtcaac acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc
      361 atgtacacca gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga
      421 aaagtcgata acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt
      481 caatgctaca agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt
      541 ctgtcttttt catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt
      601 gtcaatgcaa tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt
      661 tgtgcagcga ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct
      721 actccaccgt tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata
      781 aatgctagcc cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc
      841 cacatagaca atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc
      901 gatcggattc tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc
      961 tcagcaaacc ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa
     1021 ggcaatcccg caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact
     1081 ccgtgcagga aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag
     1141 aaacgctttt tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata
     1201 ggtgccactg ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc
     1261 cagaaactca ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag
     1321 caataccgac cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca
     1381 tccctccggc aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg
     1441 aatcaaaagc aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta
     1501 tgtagcctat agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa
     1561 aggctagaaa tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct
     1621 ctccgtcaga tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg
     1681 tgatcacgat cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga
     1741 tcatatattt ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa
     1801 agagcgtcat tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc
     1861 cttctaacgg tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag
     1921 cagcaatcac ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta
     1981 ccttaaaatg aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa
     2041 cataaaataa cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac
     2101 gcgcttattg tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc
     2161 aaaacaaaaa atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt
     2221 ccatagtctg gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta
     2281 tcaggagatt gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg
     2341 atgattgcag ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata
     2401 cttagttctt ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta
     2461 acattttgtc gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc
     2521 aagaaactgt tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg
     2581 atcatcaatg ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct
     2641 tttcgtttac tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca
     2701 aacacagtgg tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc
     2761 tcattggaaa acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc
     2821 gatgtgatat ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg
     2881 gaaataaatg ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga
     2941 agttctaatg cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt
     3001 ttcatcgact ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa
     3061 caataacaaa ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat
     3121 tatagatgta ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt
     3181 gtacttcatc aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga
     3241 ttggttttaa atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa
     3301 tagatgctgc ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca
     3361 tattgtatcc agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg
     3421 gatgagtcaa ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta
     3481 caaggccaga ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga
     3541 tacatggaag aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta
     3601 aaaggttgga aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca
     3661 ggaatgtatt aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac
     3721 ctaacccgtt aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca
     3781 aaaccatcgg aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat
     3841 gcccgttttt gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta
     3901 tctacatgaa aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt
     3961 tcgtttgtct gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat
     4021 tgtgcttcta cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg
     4081 attcgatgaa gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc
     4141 acttctggta tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa
     4201 aattcagaag cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt
     4261 ccgatttctt tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg
     4321 aaatctttcg ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca
     4381 aggttagaag cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc
     4441 cattttccag attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg
     4501 ctagaagcta ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt
     4561 tcgactagat cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca
     4621 ttgtcttgga tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc
     4681 ataatcatcc ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc
     4741 ttttctttga catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc
     4801 tcttctgtat aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc
     4861 gaatcgaaag gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt
     4921 gcaaaatgat cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc
     4981 aaatagcctt gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc
     5041 ccgctcgcta aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact
     5101 tctatctctg tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg
     5161 aatgtctcat cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca
     5221 cttccgcatc ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac
     5281 tttctgatgt tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca
     5341 ttcatcatac caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc
     5401 ctaaaataat aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac
     5461 caaagatcga aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa
     5521 ataagtattt ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt
     5581 aattcttatc gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc
     5641 tgagcacggg tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc
     5701 tttggtacat gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat
     5761 cgaattatcg cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag
     5821 ctatgatgaa tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt
     5881 aaagcctcga ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat
     5941 caaaaaaaag cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct
     6001 ttcacagcag attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac
     6061 cttgtttttc tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa
     6121 aaacagtcga tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt
     6181 tctacctttt cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg
     6241 aagtttgttc aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat
     6301 tcttttgcaa tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga
     6361 caacgtttct atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta
     6421 accaaccacg agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct
     6481 ttagctgtca aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca
     6541 gcttatttga aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac
     6601 gagcatgttg ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc
     6661 gatgctgagt atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc
     6721 cacttttctg caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc
     6781 gtccaggaaa attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt
     6841 tcatatagct atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat
     6901 ctaaaaaaag tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg
     6961 acggaaatct gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa
     7021 gaagagacag gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa
     7081 aaatcaaaga gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc
     7141 tgcaagccat cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc
     7201 tgttcaatca atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga
     7261 atgatacaat cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt
     7321 tttccaatga tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa
     7381 tgaatgactc tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag
     7441 aaaatggaag aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt
     7501 ttcaatctgc gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg
     7561 cacaccacgg attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac
     7621 tgaaaaatac attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac
     7681 tagcgaatct ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg
     7741 aagaacagac gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt
     7801 tcaaaaaact aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc
     7861 ctctgtaccg ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc
     7921 aaatgggcgt gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct
     7981 cattggtatt tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc
     8041 taaaacggct agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag
     8101 tccaattgct agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac
     8161 agacgatcca agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga
     8221 tacagcccca ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa
     8281 aaacagatga agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg
     8341 ggaaaaaaga agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg
     8401 atcttttgag tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac
     8461 tgcagtcatt atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc
     8521 atgcttttca caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc
     8581 ttcttttatt cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa
     8641 aaaatgatca tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt
     8701 atggcgatgc atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga
     8761 tttgtctacc gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc
     8821 gctgattttt ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg
     8881 tctcttctac tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct
     8941 tgatttcttg gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca
     9001 cgaaaaaata ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga
     9061 tttcccgcca gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta
     9121 gtgtcaagat gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac
     9181 cgacgatcca aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct
     9241 ccaatccaac atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat
     9301 gttctgctac atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag
     9361 gataatgcgt ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt
     9421 ttgtaatctt tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac
     9481 ttttaacttc gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt
     9541 ccaatgaatt ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca
     9601 aacaaacagg gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg
     9661 cctgataagt gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct
     9721 ttctataatg gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt
     9781 cgcactttct tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt
     9841 ttttccaaga gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca
     9901 gtccgcttct gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc
     9961 tgcactttgg acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct
    10021 taaaatatct gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc
    10081 aatcgacaat tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa
    10141 acattgagct aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa
    10201 aatgctagcc atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg
    10261 gataagagat tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag
    10321 cagctacttg cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat
    10381 gcagaggtgt cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca
    10441 ttttctgtaa ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt
    10501 ctgcgtcttt gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca
    10561 attcatcaat cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg
    10621 tcttgacact taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg
    10681 cgcccatctc ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt
    10741 ttaacaacag ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg
    10801 aaatatcacc tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga
    10861 tcgttttatc ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt
    10921 tacgggaacg ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca
    10981 gcaaagcaga tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag
    11041 agcgggttcc tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag
    11101 cgcccatctc gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat
    11161 ccccgaacag agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg
    11221 tcgtaccgga gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc
    11281 gaccatgaat cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg
    11341 catgtaaagt actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt
    11401 cagcaattgc tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa
    11461 cagtccctct taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga
    11521 tccagcaatg ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt
    11581 ggtttttaaa actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca
    11641 aattaatcaa agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag
    11701 gttcatctgg cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga
    11761 caggcaactg gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt
    11821 tctcagtcat gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt
    11881 ccaataatat ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag
    11941 cgatcctagt gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt
    12001 gattgaattg ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta
    12061 gcattcctgt tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc
    12121 cgcaaaatag ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga
    12181 tgtaataggc attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac
    12241 cagccattgg atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt
    12301 gctgcttcgt actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa
    12361 ggacatcaag ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt
    12421 gttctgcacc tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt
    12481 gctcacgggt ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac
    12541 gaatacaaag tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg
    12601 ccatcttatt tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca
    12661 gctttcaact caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca
    12721 gtggctacaa cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt
    12781 tctacacttg ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac
    12841 aatgtaggaa tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg
    12901 ccttcgaagc cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg
    12961 atctcatcca tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct
    13021 acgcctttaa aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg
    13081 tcccattgta cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt
    13141 cctccgatcg tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca
    13201 actgctggat caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc
    13261 gagatatttc caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg
    13321 atgatgtcaa gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc
    13381 gtctctcttg cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata
    13441 ccgcctacca aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct
    13501 ttttcaggca cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct
    13561 accactaaag tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga
    13621 acacgatctt tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg
    13681 agttcgtgat tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg
    13741 gctgttaatt ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc
    13801 taagtaatct ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc
    13861 tagcaagatg atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa
    13921 taattcatct ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct
    13981 ttctaaagca gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat
    14041 ggctaccatt ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt
    14101 ttcaattgca tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa
    14161 gacatcttct tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc
    14221 atgttccaga agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc
    14281 tgctatagct gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa
    14341 agtttttaga acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag
    14401 gtttttcgct ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc
    14461 aagtagcgta gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa
    14521 tgctgctaga tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa
    14581 atcattatct gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc
    14641 acccgccggt acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat
    14701 tttttctgca tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct
    14761 ccaaatcgtt tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc
    14821 gtatttttgg ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc
    14881 tggaattaat cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc
    14941 atcatgcgtg agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca
    15001 ggatcaggat ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc
    15061 attggtggta caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa
    15121 tgagttaatt ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt
    15181 tcgtaagtcc gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg
    15241 tattctgcag cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca
    15301 ggtttacctg ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca
    15361 atctgtagga tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca
    15421 gtaatcactt tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct
    15481 tctaaacctt ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt
    15541 tttgcgcccc cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata
    15601 gaacaaggac cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc
    15661 acgactgtat tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc
    15721 acagcatttt ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca
    15781 ccttttagtc caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct
    15841 ttttcgatat gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt
    15901 actatcattg tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta
    15961 ctttttcaag gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt
    16021 ttcgctcctt ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg
    16081 aaattgcctc tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa
    16141 tagccactat agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca
    16201 tcatagatac aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa
    16261 atagctaaag taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc
    16321 ca
//
LOCUS       DAGCYL010000051        15977 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.052, whole genome shotgun sequence.
ACCESSION   DAGCYL010000051 DAGCYL010000000
VERSION     DAGCYL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.052"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(233..1903)
                     /locus_tag="MB216_001244"
     CDS             complement(233..1903)
                     /locus_tag="MB216_001244"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBT4526090.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGIIAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNEWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            2085..2318
                     /locus_tag="MB216_001245"
     CDS             2085..2318
                     /locus_tag="MB216_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBT4526091.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(2417..2848)
                     /locus_tag="MB216_001246"
     CDS             complement(2417..2848)
                     /locus_tag="MB216_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBT4526092.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            2927..3340
                     /locus_tag="MB216_001247"
     CDS             2927..3340
                     /locus_tag="MB216_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBT4526093.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(3378..4526)
                     /locus_tag="MB216_001248"
     CDS             complement(3378..4526)
                     /locus_tag="MB216_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBT4526094.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFDDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(4648..5244)
                     /locus_tag="MB216_001249"
     CDS             complement(4648..5244)
                     /locus_tag="MB216_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4526095.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(5444..8086)
                     /gene="alaS"
                     /locus_tag="MB216_001250"
     CDS             complement(5444..8086)
                     /gene="alaS"
                     /locus_tag="MB216_001250"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBT4526096.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDAPTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            8474..8905
                     /locus_tag="MB216_001251"
     CDS             8474..8905
                     /locus_tag="MB216_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4526097.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMEKSLA"
     gene            complement(8950..10299)
                     /locus_tag="MB216_001252"
     CDS             complement(8950..10299)
                     /locus_tag="MB216_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBT4526098.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(10369..11532)
                     /locus_tag="MB216_001253"
     CDS             complement(10369..11532)
                     /locus_tag="MB216_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBT4526099.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKLAFDSGTVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            11661..11984
                     /locus_tag="MB216_001254"
     CDS             11661..11984
                     /locus_tag="MB216_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526100.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPQALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(12166..13515)
                     /locus_tag="MB216_001255"
     CDS             complement(12166..13515)
                     /locus_tag="MB216_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4526101.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETRGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13584..14537)
                     /locus_tag="MB216_001256"
     CDS             complement(13584..14537)
                     /locus_tag="MB216_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBT4526102.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWDLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDAMTKDMLEKLKEKLVETV"
     gene            complement(14639..15808)
                     /locus_tag="MB216_001257"
     CDS             complement(14639..15808)
                     /locus_tag="MB216_001257"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBT4526103.1"
                     /translation="MYLEKELDQLKEKGLYNTIDILESENGACIIVDGKRMINLASNN
                     YLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGTEAAIAFQ
                     SGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKDLEKKAKE
                     AIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLVTYVDDAHGSGVTGKGAGT
                     VKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSLTPGAAAA
                     ALASITLMQEHPELVEKVWENANYFKEELKKAGYNIGMSETPITPVILGDEKVTQTFS
                     KKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKEMEII"
BASE COUNT         4635 a         3371 c         2774 g         5197 t
ORIGIN      
        1 ggtacaagat catcaattgt taacatagca aattgatttc taccttcaat cgattgtttt
       61 ctcatcatta gaatcactcc cagaatagtt tatacaatga gtataaaaca aaaaaactgc
      121 aaacaacact cccaatttag gagtttgtct acagtctgga agctgtagtc aaaatacagc
      181 tccttcgatg tttaatgaaa gttatttagt atacggtaga ttataatcaa gcttaaaaca
      241 atcccaacac ttctccgtca tttgtcacgt ccatattcaa tgcagcaggt tttttaggca
      301 aaccaggcat cgtcataaca tctccagtca aggctactaa aaatcctgcc cctaattttg
      361 ggatgatttc gcggatcgtg atggtgaaat cttccggtgc acctagcagt gaaggatcat
      421 cagaaaatga atactgtgtt tttgccatac aaatcggcag tttgtcccat tcattcttcg
      481 taaactcttc tatttgtttt tgcgcttttt tgctgaaaac aacaccattt cctccgtaaa
      541 ttttcttgac gattgtttct gtcttttctt gtatcgtttc ttctaggcgg tataatggct
      601 tgtaattagc ttcttttcta tcaatcaatc gtactacagc ttttgctaaa tcaacgccgc
      661 cttctgctcc atctgcccat acactcgctc tcttggcaat aatcccctga ttttcacaca
      721 agtgctccaa cagtgtgagt tcattgtctg tatctgttac gaattcattg attgcgacga
      781 tcacaggtag ctgatattgt tccatattac gtatatggcg ctttaaattt gcaaaaccta
      841 ctttcaaagc atcaagattc tcatttttaa gttcatcttt tttcattccg ccatgcattt
      901 tcaatgcacg gacagtggcg acaatcacca cagcatccgg cgcttttttc aagttaggta
      961 ctttgatatc taaaaatttc tctccgccaa gatctgcacc aaatcctgcc tctgtgacaa
     1021 cgtaatctgc taatctcaat gctgtcttgg tagctaaaat actattgcag ccatgagcga
     1081 tattggcaaa cggtcccccg tggacaaaag ctggtgttcc gtaaattgtt tgtacaagat
     1141 tcggcttgat tgcatctttc agcagtaatg ccagagcgcc ttctgctttc aagtctccag
     1201 cagtcacagg ctgacggtca aatgtatagc cgacaacgat ccttgcaaga cgagccttta
     1261 aatcttcaag gtctgccgca agacacaaga tagccatgat ttcgctggca actgtgatat
     1321 caaacccatc ttctcttgga acaccttgga ttggcccacc caagccaaca attactttac
     1381 gcagttcacg atcattgaga tcgactaccc gtttccaaat cactcggcgg gcatcgatgt
     1441 tcaattcatt tccttgatga atatgattat ctaaaagagc agataatgca ttattagctg
     1501 ttgtgatagc atgcatgtca ccagtaaaat gcaagttgat atcttccata ggcaatacct
     1561 gtgcatatcc tccgcctgca gcaccacctt tgattcccat cacaggacca agtgagggtt
     1621 ctcgcaatgc aataacagat tttttattga ttttattgag cgcgtctcct aaccctacag
     1681 tgatcgttga ctttccttcg ccagctggtg ttggattgat ggaagtaact aagatcaaat
     1741 gcccctcagc gttatccttt gatcgattga ttccagaaaa attgattttc gccttatatt
     1801 tcccatacag ttccaaatcg tcttccttaa gatctaattt actagccact tcaacaatcg
     1861 gttctagctc tgtttcttgt gaaatttgca gatcattctt catttgatta ccccttttca
     1921 cgaactttat tcaacaaaat atcttctttg ttaagatatg ccaattatac ctttttttat
     1981 aataaaaaaa acataaaatt acaggttttt aatttatttg acgtacttat aaatactctt
     2041 tataatgaag gctatgatca gactttagaa aggtcgtgag tgctttgacc acaggaattg
     2101 taaaatggtt tgacaacaaa aaaggatacg gctttatcag ttatgatgac acagaagaaa
     2161 tttttgtaca ttttactgcg attgaagaag agggattcaa aactttggaa gaaaatcaag
     2221 tggtcgaatt tgagatcatc gaagggaatc gaggaacaca agctgcccat gtcaagaaag
     2281 gcaacgttca gactaccgat actagtatgc atgaatagat aaacaaatag agtgagacgc
     2341 taaacgggat gaaccgtacg tctcactctt ttcttttcct gtaaaaattc gtttttttag
     2401 gttctgattt ttaaaattag ctttttcttt ttttcgtata aggataagga attgattctg
     2461 atttgggtcc gtttttttca aaatgatcaa aaaggactac ttgatctttt tttcgcatca
     2521 tgaaaacaga tggggctttc tcatcttgca acatcatttg caccccagta ttagttggcg
     2581 tcaaagatcg aatctgtccg acagatattt ctcggtcttt ccaaaaagca gcataacgta
     2641 taatcacaga gtttgatttt aaaataaaag aacgtcgcaa acctagaaat agaaaaacaa
     2701 gaaaaatacc taaaacaaaa ttgcttttcc aataaggacc tgtgttttct aaagaaagaa
     2761 tcagactaaa aaacagaatg atcaaggtca atgaccaata gatgatcgta tgtgccagtt
     2821 caggctgcca tcgaatattt tttttcattc tctaacctct caccgattca gtaatgatat
     2881 aatcttacca taacattagt ttgaacaaaa aggagaatct ctttttatgt taaaagtata
     2941 cattgatgcc tctacaaaag gcaaccctgg tcctagtgga ggcggaatct tgattgtcta
     3001 tcaaggtaaa caagaacagc tatctatccc gctttctata gggacaaacc atcaagcaga
     3061 gttcgaagtc tttttaaaaa ctttagaatt tctaaagaaa aacgacttac agaatgaaac
     3121 gattttctgt ttttcagaca gcaaaacact agtttctacg gtcgacaaaa acgcaacgaa
     3181 gaacgagctc ttccttccct accttcaaag aatccaagaa cttctatccg aattttcttt
     3241 attgatcctg caatggttgc ccgaaaacaa aaataaagga gcagataatc ttgcacgtca
     3301 ggctctccat aaacaattaa aagaaaaaaa gactctttaa attaaaaact ttaaggagtc
     3361 tttttatagt ttctcattta atataaaaga taattgtgat caaacgtaat ttccaattgg
     3421 taatctccga ttttttcttc tatttgacta cgtaagattc tttctagttc tgtatcatca
     3481 aactggaatt tctcagggac cactacatca aattgcagcg tttttccttg atgaattact
     3541 ctaaaatcat gaatattaag ccctgtacct gtttcaagaa gaatttgctt taactgccgc
     3601 aagatattat agtatttttc gttatcaaca ggcattggat ccaagtgaca gacaagttcc
     3661 acatccaaag actttttaaa gtctttttct atcagatcaa taatcttatg agccacattc
     3721 aaattcattg attcatctac ttcgatgtgg actgatgcaa agcgtttatt gggtccatag
     3781 ttatgcacca ataaatcatg atagccaagt atatcatcgc aagcatttaa atgctcttcc
     3841 atttcttcaa tctctgctgg cgttgggcga cttcctaata gttcgtatac aaaatttcga
     3901 agcatcgtca atccactata aaggatatat aatgctaaag caaaacctat atatccatcg
     3961 atacgccatc cagttaccca ttcgattgct gctgaaagca atacagccag tgtagtaaag
     4021 acatcattat aactatctgt tgctgtagct ctcaatgttt cagaatcaat ggattgcgca
     4081 ataacctgat acattttgcc ttgtgcaaat ttcaaaaaga tggaaatgat taggacaaaa
     4141 aagacgatgg gtgttaaaga gacattttct ggatggatga ttttttcagc agaagactct
     4201 aaaaattgaa atcctacata agtaatgatg atcgaaacaa ataacccact gatatattcg
     4261 aagcgttcgt gtccataagg atgttcctga tctgctggct ttgcagcaat tttaaatccg
     4321 atcaaagtca atatggatga agcagtatct gatagactgt tcactgcatc agccataatg
     4381 gagacacttc ctgaaaaaag cccgatcatt agcttagaga caaacaagat cagattcgta
     4441 accaatccta agatacccgc tagtactcct ttttttgtcc gctgttgcgg tatttgtttc
     4501 tttttttctt cttgtttctt agccaagttt tttacccctt ctagtttgct tgttgctcag
     4561 gtaaaaagat ggagaggacg attgttttcc tctcctctct tcctttttct atctatcgtg
     4621 cgtcctatta ctattaaaag atggtcatta ttctggtttc aacggtgttg ctgcaaataa
     4681 cgcttttgct agtaatggtg ttatgattcc cgcacctatt gcttctggta tcccattgat
     4741 tgctgcaacc cctgctaagg ttttaaataa aagagcagaa tctacaccat aggcatctgc
     4801 cgttgcacct gtataaaata atcccatgaa tcctaacact aagactgtat tgaccaatga
     4861 acccagtacc cctgcaaagg ctgagctgat cgttattgat ttattttttt gataaatcca
     4921 gtggaaaacc aagcctgcta ctaagccaac aagtactctg ggaactaccg agataatcgg
     4981 attcgtgaac actaatgtat caaatggcgt tgttggcatg gcataggcac gaacaattgt
     5041 agcgattccc caaaacaagc caatcaccat gccatctttt gggcctaata ctactgcagc
     5101 aataattact gtgatatgga taattgttaa actaatgaat cctaatggaa taaaccccag
     5161 ccaaggaacc atcgcttgta cgatgatgat tgctaacaag atcgcacgta agactagtcg
     5221 aaaggtctta tttctactat tcattttttc accccttttt atttatacta atcgtaaaaa
     5281 tttctgtcac tttcgtcaac taaagaccat ccgaatccga ccatttgatt agatgcgcca
     5341 gccaattgaa aaaagtatga gggcaagact tgtttgtctt cgcccccata atcatgtttc
     5401 gttcaatgtt tgaacttttg tttttctgtc aaacgtaaac gccctattgt tgcgcaaccc
     5461 attctttcgc agctttaagt gcatctggaa taccagcagg atttttcccg ccagcttgtg
     5521 ccatgtctgg acgtccacca ccgccaccgc ctactttcgg tgcgattgct ttaatcaagt
     5581 cgccagcttt tagtccagct tcatttgatt ctttcgtcat tgccgctagc agactgactt
     5641 ttccttcttg ttcgtttgct aaaacaagaa tatctgaaat atttttttgt ttccattggt
     5701 cagctaattg acgcagctga ttcatatctt tcacttttac ttgtgctgca atgattgttt
     5761 ttccattgat cgtttctgga tctttgaata catcaccagc ttgttgattc gctaacttgc
     5821 tagctaattg ttcattttcc ttttgtagct ctcttacttg ctgttgcaat tgttccgctt
     5881 ttgaaacaac ttccttcaat tgaggtgatt taacaattgt tgctacttgt tttagttgtt
     5941 tttcttcttc ttcaagtagt tcgtacgctt ctttacttgt tacagcctcg attcgtcgca
     6001 cgcctgcacc aattcctgat tcagaaacga ttttgaagat tccgatgttt tctgtatttg
     6061 ctacatgtgt gcctccgcac agttcgattg accagccgcc aatatttact actcgaacgt
     6121 catgtccgta tttttcacca aacaaagcca ttgcacccat gtttttggct gtatcgatat
     6181 ctgtttcaat tgtttcaact gggatagctt cccagatttt ttcgttgacg attctttcca
     6241 tttcagctaa ttcatctgaa gtgatttgac caaagtgggt aaagtcgaat cgtaaatgtc
     6301 caggagcgac taaagaacca gcttgatttg catgctcgcc aagtacttct ttcaatgcct
     6361 tgtgcagtag atgggttgct gtatggtttt tgatgatccg attgcgcatc gattgatcaa
     6421 tcaatagatg ataggtttgt ccttcactca ttggtgcttt tacttctacg gtgtgcataa
     6481 attgaccatt aggcgctttt tgaacatctg taacaactgc tacgatttcg cctacttcat
     6541 tcatgatcca tccttgatcc gctacttgcc cacccatttc tgcatagaaa ggtgtttgat
     6601 caaagatcat ttgagcttca ccttctgcga cttcattcaa aagagactca cctttaacga
     6661 tgaccaataa ttgactatct gattcttgtt ctgtgtatcc aatgaattta ctttctactt
     6721 tgatatctgt caataaagca gattgtaccc ccattgattt ttcagtactg cgggcagagc
     6781 gagcacgttc gcgttgtgca tccatttctt tttcaaaacc agcatgatca actttcagtc
     6841 cttcatcttc tgccacctct tccgttaatt ctaccgggaa gccatatgtg tcgtatagtt
     6901 tgaagatgtc ttttcctgct agagtcgtcc cgttttcgtt ttttacttct tggatcactt
     6961 gatttaagat ttccaaacct tcattgattg tttcattgaa acgttcttct tctgtacgga
     7021 taactttttc aataaattct ttttgttgaa gtacttcagg ataataactt accatgattt
     7081 ccccaacaac agggactaaa cgatataaga atgcttgttc aatacctaat tttttcccat
     7141 gcatgacagc acgacgaagc aaacggcgta agacataccc tctcccttca ttcgatggca
     7201 atgcaccgtc gccaatagca aatgaaaggg cgcgaatatg atctgcgatt actttgtaag
     7261 aaatatctgt tactgggttt tcaccatatt ttacttgttt gcttagcttt tctacggcgt
     7321 ggataatagg cataaacaag tctgtctcaa agttcgttgg cgcatcttga acgattgaga
     7381 ccatgcgttc caatcccatc cccgtatcaa tgtttttatg aggaagtggt tcaaatgtgt
     7441 tatcaggctt atggttgaat tctgaaaata ctaagttcca gatttctaaa taacgttcat
     7501 tttctcctcc tggatagttt tcaggatcgt cttctgctaa atcgttataa gcttctccac
     7561 ggtcatagaa aatttccgta tccggaccac atgggcctgc accgatatcc cagaagttgt
     7621 cttctacatc aataatgtga tctagtggca ggccaacttc ttcatgccag atgcgttttg
     7681 cttctgtatc ttttggataa actgttacat aaagtttttc tggatcgaat gccatccatt
     7741 ttggatcagt caaaaattcc catgcccagt ggatggcttc atttttgaaa tagtcaccaa
     7801 tcgaaaaatt acccagcatc tcaaacatcg tatggtgccg tgctgtttta ccgacatttt
     7861 cgatatcatt tgttcgaata gatttttggg catttgtgat tcttggattt tctggcacaa
     7921 ctgaaccatc aaaatatttt ttcaatgttg caacaccaga gttgatccac aataaggttg
     7981 gatcgttcac ggggatcaaa gacgcactag gttcaatcgt atgtccttta gattggaaga
     8041 aatcaagaaa catttgtcga acttgtgcac tacttaattg tttcatgtca ttacctgctt
     8101 tctataaatt aaatacaaaa aaacaccatc acagccaagg acgaaaactc gcggtaccac
     8161 cttgattgca atgatgtgta atcattacct cttaaactcg ataacgctga gctgcgtcgg
     8221 tatttctacc aaaatagaaa aagggagcaa tttgattagt gactattctt ttttcagcca
     8281 agaaagaatt ttctgtgagt cttaaaaaac aaatcatatc cttttcgtac ttaattagta
     8341 tacgcattct attgatcact gtcaatatct caatttttat tttcatttcc ttttggtcat
     8401 actccacgat ttttggtacg ataagaataa aggaatcaat acatctctgc tattcatgac
     8461 aaggaggaat atgatgaaaa aaatagttgg agcggatgaa tggatacgcg cagctagctg
     8521 ctttttgaga tatcaagttt ttgtattaga acaagggatt cctgcagaac tagaattcga
     8581 cggaaaagat atcccgcaac aaacctatta tctatttctt gatcaaaaaa gaccagtagc
     8641 tactttgcgt tatgaaaaga aaacacctac ttgcttcaag ccggaacgat tttgcgtagc
     8701 aaaagaatac cgaggaaaag ggttaggcgg tcagttgctt gaagctgctg aaaaacgggc
     8761 ggtttcagaa ggttgtcaga ctagttatgt agtagctgaa atgacggctg tagattttta
     8821 tgaaaaacaa gggtatcata cttgcacaga accttttatt gaagatgaag tactttgtat
     8881 cggaatggaa aaaagtctag cttaaaaaca agagattgtg acacgccttt tgtcacaacc
     8941 tcttgttttt tatttatgtg aatttttctt atttttacga gcagtacggc tttgctgtct
     9001 gcgttcgatt ttgcgttctt ttttattttt ttctgcaatt gcccaatcga tttttttctt
     9061 ataaccaggc ttgatctttt tctttttctt cttcacaaga ccaatcagcg tgggatctaa
     9121 tacatctttt gatttttctc gtttggcacg tctattacga tcgtatgttt tgacgatttc
     9181 cccgtttttg atttcttttg gttcaaatga gacgcccaat ttttctatcg catcaatggc
     9241 ttgctcatca tttggcgaat ataacgtaat ggccgttcct tttaagccat tacgtccagt
     9301 acgtccgaca cgatggataa agaagtctag atcttctgga atttctgcat tgataacatg
     9361 agaaacacct tcgatatcga tccctctagc agctaaatca gtcgctacaa catattgata
     9421 ctccagattc tgtacttgtc gcatcactcg ttttctctct cttggggtga tgtctccatg
     9481 aatctttgct actttcaagc cttggttttt caagtaatcg gcaatttcat ccacatgctg
     9541 ttttgtattg gcaaatacga ttgctaaata aggatgacca attgtcagca gttgataaat
     9601 catttgattc tcatctttac cttttgttga aatcagccaa ttttcaatgt cttcagaaat
     9661 aactgctttt ggtttgattt cttcaatgat tggattttcc atgtattttc gtaagaatgg
     9721 acgaagtttt tctggaatag tggcagaaaa aactaaaaac tgcagctttt ctggtaatcg
     9781 acttgcaatt tggtctactt catttaaaaa acccatatcc aatgtcatat ccgcttcatc
     9841 gacaacaaat gcaaatgcgg tgtgaatctt cagcgcttgt tcattcatca tgtctaatat
     9901 tctaccaggc gtgcctacta cgacctgtgg ctgttggtga tgcagacgat ttaattgacg
     9961 ctgtttgtct gtaccgccga caaaattcga gacacggatc tccggtgtag aaaatttagc
    10021 gatttgtgta gctgcttcat agatctgtgc agctagttcc ctacttggag ctgtaataac
    10081 aacttgtact tcctcttttt ctgcattgat tttttcaaac aaaggcaaca aaaacgtatg
    10141 tgtttttcct gtaccagttt gagattgccc aatgatattt tttccttttg caattaaagg
    10201 aatcaaacgt tcctggacct ctgtgggctg tttgaagccc tttgcggtta acgcttcatt
    10261 gataaacggt ttgaagcgga atatatcaaa attattcatt cgtgcacctc ttctctttaa
    10321 attatcacga ctttggcatt ataacacaga caaagggttt ttgctcagct aatttcgaga
    10381 atcatcatcc tttgtttggc caatcgtgct aacagtatat gtgtgactag ctttttcata
    10441 aaacagaatc cgtttttttc tcaaaaatga tagcaagata tttaaaataa aaataccttg
    10501 cagtaacaag acaccgagag aaatgaatac tgcactcatc tgcaacaccg tattcgttgt
    10561 cgaattcatg atttgctgaa gcaactgccc caaccagctt aaaccataaa aaagaaagta
    10621 cagatacccg tagcggatca tcaatgcttt taaagaaatt cgttcgcggt cttcttccac
    10681 caaacggata cgcacaattt ttttgccaag tgtccatcca ttggttataa acggtacaat
    10741 cataaagtat acccctactt ctaataaaag ccaccagata tgagaagtaa ctgtccctga
    10801 atcgaacgca agtttaaaga aaatcgccgc taccaaatta ataagagata aaaatgtcca
    10861 gtcgatcaaa taagcgacca agcgtcgagt caatgagaca gtctttcctt tttcataaga
    10921 aagttcatcc attcgtcttc ttgtaggaag cataaatgta aataacggcg taacgacaaa
    10981 accaatgatt ccacccaatg tatttgtcac taaatcattc acatcaaata agcggtatgg
    11041 tcttgggtag ataaaataaa gtccacttag ctgcgtcaat tcaaaaaata gagaaaggca
    11101 gaaactagca atcatcgttt tcataaaaga aagccggaaa taatatctta aataaatgcc
    11161 aaatggtatg agtaataaaa cattaaaagc tggttccaaa aatacactct gcttcattgc
    11221 cggcaggtag gtactaggtt cagacaagac caataccgtg ttgtctctga aatgttgcca
    11281 tgaagcaaat aaatgcagct ccatctttgg cccagtcatt tgggcaaccg tctcctttgc
    11341 cggaagtggt aagatcacca aaaaatacgc acacaataaa taaaaaacga aagaatacaa
    11401 aataactgcc cttgttacta gaaatgttcc atactttcga tattcataaa taaaaaaaac
    11461 agatgagatc gctaaggcta aaaatggaaa aacaatcagc gcaaaacgaa tcggttcgat
    11521 ataacttgcc atatgactcc tccttgattc tttgtatctc tcttatctta gcagtccttt
    11581 gctgtttcaa gcaagtaacg aggctattat ttctcatttc tttcaaagta aagtaagata
    11641 aacacaaagg gggtgtcgag atgctgaata tgtactttgt ttttggcgta cctatttttt
    11701 tactgttcct ttatgctacg atcgcttatg taagaaaaag aactacaatt cattatttag
    11761 gatttattct cctgattatt tcgggtttca tgctagtctt caatcttcag acttggcagc
    11821 aagcacttct tgaaatggat aaaatgactc cacaagcttt gtctaaagtg cttggctacc
    11881 ctgtctatct gatttggctt cccattttca tttctggttg cttggtttta ttaaatattt
    11941 atcgcggcgt acgccgaata gttcagttac gcaaatcaaa gtaaaaggca gaaatcggtt
    12001 gagcccgatt tctgtctttt actttgctat ttcattgtag acaaaacgac atttacgtga
    12061 tcaaaattaa cgagtacttt tgacatttct tccacgtact cctcatttaa ataaaatatt
    12121 ttgtttggca gagagtgcag ttctaataaa attcgaatgt tttagctatc tacgttttaa
    12181 atagagataa atcgggatac cggcaagtgt caatactatc cccatcgaag ctaaaaaggt
    12241 ctgagtgata agcgtgcttg atacgataaa agtccctcca atgattgcta ctagtggaat
    12301 aaacggatac attggaactt tgtatggtcg aaacaaatca ggttcttttt tacgaagaag
    12361 aatgacacca acaaagacca tcgtataaaa aatccaaatg acaaagatca gcatatccgt
    12421 tagtgtatca aaaccaccga tcatcatcat gccaatagcg atgactagct ctaagatacc
    12481 tgcggctaca ggaactttag ttttatcatg aagtttagca aagagcttgc taaaaggcag
    12541 gttgttttct tttgccataa cataagggag acgcattcca gtaagggtat agccattgat
    12601 ggttccataa acagaaatca agataccaac tgtgaccagt cttccaccga agccgccaaa
    12661 aatcattttt gcaacgtcac ttgccgcatt gctgtttcct gcgactccgt cgatagatgc
    12721 ggttcttaag aacaccgcat ttactaataa ataaacgatc atgattccga tgattcctaa
    12781 agaaattgct tttggcaaat ctttcgccgg ctttttcaat tctcccgaaa tatttcctac
    12841 gtgtatccaa ccgtcgtatg cgaacatcgt tgctagcaag cccgctccca aggctgagaa
    12901 aaatgaaaga ttttctcctg cttggatcgg aaacagttgg aagtctactc cttcttgtcg
    12961 gaacaaaccg aaaatgacga tcacaaacaa agggataagc ttgcagacaa gcgtaatcga
    13021 ctgaaaagcc cctccagctt tagaacctaa aaaattgatc agcaggatcg atacagcagc
    13081 tgtaacagct acgggaacaa tcattgactg ggaaagacca aatagattga cgaactgtgt
    13141 accgaaaatg atcgacaagg ctgctacatt tgctggaaaa taaatcacga cttgcgccca
    13201 gcccaataaa aaagctgctg tatttccgta aatccgttcg atatatttaa tcatccctct
    13261 tgtttctgga atagcagctg ccagttcagc tcctgttagt cctgcacaga cagaaatcat
    13321 accaccgaga aaccaagaaa acatatgcaa actggcggat cctgtaactt ctgctacgct
    13381 tgcggcttta aaaaataccc ctgcaccgat cactgtgccc ataacagtcg ataaagcagt
    13441 aaaaaatccc atcgtacgtt ttaattcatt ttctattttt acttctttta ctggcgtggc
    13501 ttgatattct tccatccctt gtcccctttc ataccatagc caatcccaaa agtataaaaa
    13561 actttttgga tgtggttctt gttttatacc gtctctacca atttttcttt cagtttttcc
    13621 aacatatcct ttgtcatcgc atctaagtcg tatttcggcg aaaagcccca ttcctcttgc
    13681 gcacaagaaa catctagtga atttggccaa gattctgcta ttgactgacg tacaggatcc
    13741 acatcataat ccatttcaaa atctggtatg attttttgaa tagatgcttt gatctcttcc
    13801 gggtcaaaag acatcgccga tatattgaag gcatttcgat gaaccaatcg atcaggatct
    13861 gcattcatga gttttataat ggcatcgatc gcatctggca tatacatcat atccattgat
    13921 gtaccttgat caatgaaact agtgtacttt ccttctttga ttgctgaata ataaatatcg
    13981 actgcgtagt ctgtcgtacc gcctcctggc aaggttgcat aagaaatcaa tccagggaaa
    14041 cggacaccac gagtatctac gccatatttc acataataat aatcacatag tagttctcca
    14101 gctaccttcg ttaccccata catggttgta ggacgctgaa gagtatcttg tggggtatga
    14161 tcttttggag tatttggacc aaacgcgccg attgaacttg gcgtaaaaaa tttcagctgg
    14221 aattctctag cgacttccaa tgcattgacc agcccagtca cgttcaaatc ccaggctaat
    14281 tgtggttttg cttctgcaac agctgacaaa agagcagcca aatgaatcaa tgtgtccact
    14341 tgatattgtt ctgctatgct gcgcattttt tcgtaatcca ttacatctag gatctcaaag
    14401 atatcgtttt tctcaatgac ttcaggcatg cgtatatctg tcgcaatgac attttcactg
    14461 cctagttctt gacgtagtcg tgtgatcaac tctgaaccaa tttgtcctaa acatcctgtc
    14521 accaatactt ttttcatgat gatcctccct gatctttttt actactatct tcagatgatt
    14581 aacaatatat gcgtgaacat gttctctaat tttaaataga cgaaaaaact aaatttagtt
    14641 aaataatttc catttctttt cctatttttt gataaactgc tactgcttca tcaagcattt
    14701 ccctcgtatg ttccgcagtc ggcatatttc tgatcctgcc tgtcccaaga ggcacagttg
    14761 gataaacgat tggtttagca taaatcccat gttcgatcag cttcttagag aaagtctgag
    14821 tcactttttc gtctcccaaa atgactggag taataggtgt ttcagacata ccgatgttat
    14881 agcctgcttt tttcaattct tctttaaaat aattagcatt ttcccaaact ttttcgacaa
    14941 gttccggatg ttcttgcatc aaagtaatcg aagccaatgc agctgcagct gcacctggag
    15001 ttagcgaagt agaaaatagg aatggccgac ttctagcttt caaccagtcg attagtgttt
    15061 tcgatccagc tacataaccc ccaacaacac ctatagcttt cgataacgtg cccatttgca
    15121 tatcgatttt gtcgcttaat ccaaaatgct tgacggttcc tgcacctttt ccagttacgc
    15181 ctgagccgtg cgcatcatca acgtaggtga ccagtccata tttttcaaca atcggaataa
    15241 tttccggcaa tcgtgcaatg tctccatcca tggagaaaac accatctgtg atatacatga
    15301 tttttttata tttcttactt tctatcgctt cctttgcttt tttctcaagg tctttcatat
    15361 cttgatgttt aattcggatg atttttgctc cggataagcg gcaaccgtca ataatagatg
    15421 catggtttaa ttcatccgat aagatagcat cttcttttgt cattactgca gagatagcgc
    15481 ccatattgca gttgaatcca gattgaaagg cgattgctgc ttctgtccct ttgaattctg
    15541 cgattttttc ttctaattgc tgatggattt tcagcgaacc attgatcgtt cggactgctc
    15601 ctgctcctac accataagtt tcggttgctt caatacatgc ttttttcaat tcttcgcgat
    15661 tggcaaatcc aagataattg ttggatgcta gattgatcat cctttttccg tctacgatga
    15721 tacaagcacc attttcactt tctagtatgt cgatcgtatt gtataacccc ttttctttca
    15781 gttgatccaa ttctttctct aaatacattg ttagttcttt tggaagacat tgtctccacc
    15841 tccatattca tctgtcttgc agaacatacc aaacatatat cgtgtcattc atctacaaag
    15901 caagtatgcc atatttttga taacgcttac actgattatg agaattattt aaccacattt
    15961 tttatttaca gtatctt
//
LOCUS       DAGCYL010000052        15850 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.053, whole genome shotgun sequence.
ACCESSION   DAGCYL010000052 DAGCYL010000000
VERSION     DAGCYL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.053"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(105..629)
                     /locus_tag="MB216_001258"
     CDS             complement(105..629)
                     /locus_tag="MB216_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4526104.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="MB216_001259"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="MB216_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBT4526105.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="MB216_001260"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="MB216_001260"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBT4526106.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="MB216_001261"
     CDS             complement(2351..3031)
                     /locus_tag="MB216_001261"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBT4526107.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="MB216_001262"
     CDS             3157..4026
                     /locus_tag="MB216_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4526108.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="MB216_001263"
     CDS             4052..4567
                     /locus_tag="MB216_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526109.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="MB216_001264"
     CDS             complement(4614..5207)
                     /locus_tag="MB216_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4526110.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="MB216_001265"
     CDS             complement(5232..5411)
                     /locus_tag="MB216_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526111.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="MB216_001266"
     CDS             complement(5488..5997)
                     /locus_tag="MB216_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526112.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="MB216_001267"
     CDS             complement(6093..6473)
                     /locus_tag="MB216_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4526113.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="MB216_001268"
     CDS             6803..7552
                     /locus_tag="MB216_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBT4526114.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="MB216_001269"
     CDS             complement(7736..9013)
                     /locus_tag="MB216_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526115.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="MB216_001270"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="MB216_001270"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBT4526116.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="MB216_001271"
     CDS             complement(9935..10879)
                     /locus_tag="MB216_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBT4526117.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="MB216_001272"
     CDS             complement(10881..11567)
                     /locus_tag="MB216_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBT4526118.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="MB216_001273"
     CDS             12041..12361
                     /locus_tag="MB216_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBT4526119.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="MB216_001274"
     CDS             12515..13420
                     /locus_tag="MB216_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4526120.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="MB216_001275"
     CDS             13417..14190
                     /locus_tag="MB216_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBT4526121.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="MB216_001276"
     CDS             complement(14371..15795)
                     /locus_tag="MB216_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4526122.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
BASE COUNT         4649 a         3297 c         2812 g         5092 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gtgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt
//
LOCUS       DAGCYL010000053        15395 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.054, whole genome shotgun sequence.
ACCESSION   DAGCYL010000053 DAGCYL010000000
VERSION     DAGCYL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.054"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            132..2194
                     /locus_tag="MB216_001277"
                     /pseudo
     CDS             132..2194
                     /locus_tag="MB216_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
     gene            2277..2846
                     /locus_tag="MB216_001278"
     CDS             2277..2846
                     /locus_tag="MB216_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4526123.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3021..3827)
                     /locus_tag="MB216_001279"
     CDS             complement(3021..3827)
                     /locus_tag="MB216_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526124.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3965..5479
                     /locus_tag="MB216_001280"
     CDS             3965..5479
                     /locus_tag="MB216_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBT4526125.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5882..6865)
                     /locus_tag="MB216_001281"
     CDS             complement(5882..6865)
                     /locus_tag="MB216_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBT4526126.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMKANKNIGKIILKITE"
     gene            complement(6870..7517)
                     /locus_tag="MB216_001282"
     CDS             complement(6870..7517)
                     /locus_tag="MB216_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4526127.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7602..8234)
                     /locus_tag="MB216_001283"
     CDS             complement(7602..8234)
                     /locus_tag="MB216_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4526128.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8250..8801)
                     /gene="coaC"
                     /locus_tag="MB216_001284"
     CDS             complement(8250..8801)
                     /gene="coaC"
                     /locus_tag="MB216_001284"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBT4526129.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8798..9535)
                     /locus_tag="MB216_001285"
     CDS             complement(8798..9535)
                     /locus_tag="MB216_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBT4526130.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNCLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEETQSKSEIAALITEKIRLEESK"
     gene            complement(9532..10521)
                     /locus_tag="MB216_001286"
     CDS             complement(9532..10521)
                     /locus_tag="MB216_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBT4526131.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10779..11321
                     /locus_tag="MB216_001287"
     CDS             10779..11321
                     /locus_tag="MB216_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4526132.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11338..12423
                     /locus_tag="MB216_001288"
     CDS             11338..12423
                     /locus_tag="MB216_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4526133.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12435..13241
                     /locus_tag="MB216_001289"
     CDS             12435..13241
                     /locus_tag="MB216_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4526134.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13242..14069
                     /locus_tag="MB216_001290"
     CDS             13242..14069
                     /locus_tag="MB216_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4526135.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14066..15139
                     /locus_tag="MB216_001291"
     CDS             14066..15139
                     /locus_tag="MB216_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4526136.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4953 a         2778 c         3137 g         4527 t
ORIGIN      
        1 tgaaacaata aaaaatcaaa aaaaatactc tcctaatagt ccatcttctt ttgattttat
       61 ttatataatg aaatcatctt atgaaaatta ctacttcatg tttagacaag agtagggagg
      121 actaagaata aatgtgttca aaaagacgtt caaaacaact aaataaacac gcttgtcgtt
      181 tagataagga acggtcgtgt tctgaaaaac actacgttaa gtttatgatg cgcacgatgc
      241 tgatcagttt atctgtaggt gtctatatgc atgtacccgt tcaggcaacg acacaaacca
      301 tcaaggtcgt acgtacctct gtttctgaga ccactgattc ccctatcact attactcata
      361 ctttcactga tgcaaatgga aaacagctac ttcctgcatt gacttcctat aataaagtca
      421 gttatcctcc aacgataaac cactactggt taattcctaa caaaacatta tggacatcgg
      481 aaaccggaag cagtgtgccg ctttctatct tttccgatat gactgatcct acaaaaggtg
      541 atcagttaaa acagatcact gatattttga atcatgcttt gacagatata aaggacggtg
      601 gaagcgcaca agtttcttat gtatatgaaa aagattattc acagtttgaa agaaagaaaa
      661 tcgatgttct ggcagggaca aaagtttcga tatctgatct ggtagaaagg ctgaagaacg
      721 cagatggaac acaaggagat atgtccaaag taatttcaaa caatgggaat atcgtggata
      781 cgactaatac aggaacatct actatttcct tgtcctattt tgatccaatt gctttaaaaa
      841 caatgactgc atctgctgtg atccatgttc gtgctgatca aacagctatc aaaggaaaag
      901 atcttcactt aagaacagga gagagttacg cccctaaaga tcttgttgaa tatgtaacaa
      961 aaagcgatgg aacttcagga aataaagaag aaatcaaaat gaacgggaca gtagatacta
     1021 caactccggg agaatatccc gttgtattaa cttatataga ctcgaaaaca tcaaatgtgg
     1081 cagaaacaac agctgtagtt tatgtagaag catctgctgc ttcatcgact gtcatcactc
     1141 attttataga tagtcaaaca aataaagaac ttcaagcaga aacacatatg agtagtgtaa
     1201 atcgtattcc tgatttgcgc ctacctgctg aattttctga tcgagtgcca aatatataga
     1261 tttatcgact tatgaacaaa atgggaaagt cacttctatt tctgattgga tgcaagcaaa
     1321 cggattagac agaaaaagtc actggtcgga gatccttgaa gtacttcact cagaactgat
     1381 gaataatagg aagcaatcag gaggggaaat tacctttact tatgtttata taccagacaa
     1441 tcgttcatta gaaggtcatg atcttattgt tcatccaaac gaaaaaataa acaaagaaga
     1501 gttgatccaa tcagcaacaa attccttagg taaacccgta ccgttaaagg atgtatcggt
     1561 tacaatcaat gaagtaccat tggaggatgg ttatttgacc aaacaaacag gtgattttac
     1621 gattatgtat agttatcatg acccgtattc actccaagaa ctccatgctc agtcactatt
     1681 acatgtttta ccattaaaca aacagataga tcaaaaagaa caaaaaagac aagaagaaag
     1741 cttgtcagaa aaaaaacaac aaatggctca tgcagtttct ccaacaggga aagtaaaaca
     1801 aacaagaagc ctagagttag caagaatatc tagaaaaatc tcagaatcta ataaaacgat
     1861 aaggcaagaa gctcatttgg ccccacaagt ttctggattg gaaaatgaaa ttttagagcc
     1921 taaaataaaa caacaatcag aaaaagaatt tgttgctaaa cagtcggaag atcagaagga
     1981 atcagatgac cagaaggaaa cgaagaaaaa acgcttgtca actattgatt ccaccgatga
     2041 gcatctgcag ccgcaagtac cacttcctag tccgggaaca cccgcacctg gtggaggtat
     2101 tccttcttct gctttaggag atttcatgcg aggtatggca ggaacatggg tatacgctaa
     2161 tcgaaatgaa tttgacgcag atgactggtt ctgatttata tctctatctg ttatagtcaa
     2221 ataagtagct gagtcatatg ttatttggca taatggagac gaggagggat agaaaaatgt
     2281 tagataaaac gttaccttac gcagaaatct ggatgacaag accacttcaa caaaatgttc
     2341 caatcacgcc tttagcggaa ggttttgaat ttcgatttta tcaaaaagaa gatgaatctg
     2401 cttgggcgga gattgagact tccgttgggg aatttcaaac gacagatgaa gcacttgagt
     2461 attttcaaca aaactttgct ccttatcctg aagaacttga aagaaggatg ttttttgtag
     2521 agacaaaaaa aggagaaaaa gtagcaacct gtaccgcttg gcgaaaaaaa tcaagagagc
     2581 aagtgtatcc ggtttttcat tggttagcag tgaaaccaga ttaccaagga aaagggctcg
     2641 caaaagcatt gactgccaga gtcttgcaag aatttcctac cctccatacg gacggaccaa
     2701 tctatcttca cacacagaca tggagccatc aggcgattgc tctttataaa aaaatgggtt
     2761 ttacttttat tgctgaaaat ctagacggca cacctaatcc agattatgaa aaagttatgc
     2821 aggttttaag taaattagat aaataacgaa atggtgaaat aagtcctcag tattgggata
     2881 aaaaacgaaa tagtcattcc tcatatctta cttttttgag ttaatactat cggataacta
     2941 atggcatgcc atacattcaa aaaagaaggg gcagtgacaa aagtattgtc actgcccctt
     3001 ctcttgtttt ttagcatgtt ttatcgtttg atttttttag gaataaaatt cttgatgaac
     3061 gtaaattttt ggtaatcgtt tcgactatag aggtaaccaa atacactgaa aacaactgct
     3121 ccaataaaat taacaaataa atccttcata gtatccatga ttcccacgtc taagtaacca
     3181 ttttggatga ttgttttatc tgttttacca tctgcatttt gactttcaat gattgtccga
     3241 gtgatatctg gaacatgata gacagtgttt ccgtctttac ttaactccac actattgatt
     3301 tgctggatga tcgtatcttt ttgcatatcg aattgcagta atttatctgc tgaaaattcc
     3361 acaaactccc acataacacc aactgtcatc gaaaaacaga aggaaacaac tgctagtaat
     3421 aaaggagtca agttcatggt ttttgtattt ttattgagca aataaacaag ggaaaagccg
     3481 acacccccag ctaaaaatcc gctgaatgca tgtagaaaag tatcccacat tgaaaaatag
     3541 ccataaaaat cacttaactc tcctaaaact gttgaactaa aaataaaaga taacaaaatg
     3601 atttctagca tatttggaat ctgtatcttt aatatctttt ccagaaaata agggatttca
     3661 aataaaatta atgtcagtaa taaaagcatt gtatttcccc agtgttggtt ccagacctga
     3721 tccactagca taataacgac gatgatacgt aataaaatat agattccgtt tgtgattcta
     3781 cctgcagttt ttgtactgtt cgtaaaaaag gttttcaaat gattcataaa ataactcctt
     3841 catgtatatc cttaattata ctaagaagcg tgtgtttacc aaagctatat gacttaaaga
     3901 aaaacagagt tgatattgtg aaaaaaatca cttaatatag tataaggaga aaagggggat
     3961 acaaatgaaa aaattagttg ctggtttatt aacagccgtt gcacttcttg ctatgagcgc
     4021 atgcggcgca gatcagaaaa atacagcgca gacaagttct agtacgaagg aatctgccga
     4081 agtatcatca ggcgcttctg aacaagtata tacagatcca aaagaaatga aagaatctta
     4141 tgatgtcgtg attattggag ctggtggtgc agggatggca gcagctatcg aagcaaaaga
     4201 tgctgggttg aatccagtaa ttttggaaaa gatgcctgtt gccggaggaa acacatctaa
     4261 atcatcaagc ggtatgaatg cttcagaaac aaaatttcaa aaagaagaag gaatcaaaga
     4321 ctcgaatgat gccttctatg aagaaacgct aaaaggtggt ggaggaacga atgataaaga
     4381 aatgcttcga tactttgtcg atcactctgc tgatgcggtc gattggctag atcaaaacgg
     4441 tatcaaactg gacaacttaa cgatcactgg agggatgagt gaaaagagga cacatcgtcc
     4501 gtctgatggc tcggctattg gtggatactt agtggagggg ttattgaata acgtccatga
     4561 gcgggaaatc cctatttttg tcaacgcaga tgtgacagac attaagaaaa atggggaaac
     4621 ggtcaatgaa gtgactgtac atgttcaagg agaagaacca aaagaggtca ccggaaaagc
     4681 aatcgtagtt accacaggcg gttttggtgc cagcaaagaa tttattgaaa aatatcgtcc
     4741 cgatttagct gattatatta cgacaaatca agaaggatca actggagatg gtatcaccat
     4801 gattgaaaaa ttaggtggac aaaccgttga tatggataaa atacaaattc atccaactgt
     4861 gcagcaggat gagggggtct tgattggtga ggttgttcgc ggagaaggag caatcttggt
     4921 cgatcaagag gggaaaagat ttgtcaatga aatggataca agggataaag tatctgcagc
     4981 aattacagca ttgcctgaaa aatcagctta tttgattttt gaccaaggcg tgcgtgatcg
     5041 tgccacagct attgaatttt atgaccaaaa aggatatgtc acaactggaa agacaatgga
     5101 agaattagca gaaaaaatcg atttaccaaa agaaacacta gcaaaaacag ttgatggatg
     5161 gaatacggat gttgcggaga aaaaagatgc tcaattcaac cgtgccacag ccatggaaca
     5221 tgacttatcg tctccgaatt attatgcgat caaaatcgca ccaggaatcc attacacgat
     5281 gggcggtgtg aaaatcaata cgaacactga agtattagat aaagaaaacc agccaattag
     5341 ggggttatat gctgctggtg aagtcacagg tggcttacat ggaaacaacc ggatcggtgg
     5401 aaattcagtt gcggagatca ttatttttgg gcgacaagcg ggcatacaag cagctcgatt
     5461 tgcttcggaa aatgaatgaa atagaacgta aataaaaaga ggttgtgaaa tcagcgtttt
     5521 gagctaatac cgagggtcag ctgattgaac actgtttagt gaagaggttg tgaaaaagct
     5581 gccctgcatc aagtaataag aacagccaag taaaaacagc ttcttatgtt tttaacttgg
     5641 ttggacttat taccgcagat gcaagctttt gaacaccgtt tattcggaaa aagagcgtgg
     5701 gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaagcaga
     5761 agaaattcct tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat
     5821 ttcttcttat ttcttggagt taaacgcttc tgtcccagcc tcttttttga tcagcatgat
     5881 tttattcagt tatcttcaaa atgatttttc cgatgttttt gtttgctttc atatattcat
     5941 gcgcttgttt gactgcgctg aattggaatg tacggtcaat gattggccga atcttgccat
     6001 tttctagaaa aggcccagct ttttgcataa aagtttgtgc gagctctgct ttatactgat
     6061 cacttctagg tgtcaataat gtaccaagca gttggacacg tttcccaatc aatacaccaa
     6121 ggtctatctc aggcacgatg gtcccaccta gcataccaat caagacccaa cgtccatcaa
     6181 cggctataga agctaaattc ttttcccagt aagaagcgcc gatgaaatcc aaaataacat
     6241 cgactccccg accttcagtg atttttcgta cctcttcttg aaagtcttgt tttttatagt
     6301 tgatcaattc atctgcacca agttctttac aaaaggcgag tttttcttct gatcctgcag
     6361 taacgatgat tttcgcgtga gaaagctgtc tagctaattg gatcgctgct gttcccacac
     6421 cacttgctcc tgcatggatc aagacagttt cgccttcttg taattttcca agccagtaaa
     6481 gtgtttgata agcagtcaaa aaaacttctg agattccagc agcagaaaca tagtctagcg
     6541 tatcagggag aaggattgca cgatcggcag gcatcacagc atattcggca taaccaccta
     6601 gattgactaa tccgtatact tttgtcccag gagtaaaagt ggagatatca cttttattct
     6661 caactacctc tccagctact tcgactccca aaatagggta gggaggtttc aaagagactg
     6721 attctctttt cataatatct gttcgattga cagctgctgt atggactttg atcagtaatt
     6781 cgccttgttt tggtttagga acaggcactt caatttgttc taatacatct acagaaccag
     6841 gatgcttgat tgaaatggca cgcatggatc tattcctcct ttgcccattc ttggaatgct
     6901 gtattcaact ctttgctacc ttcagaaagt actagagtcg tacgagaaat atccgtttct
     6961 tccactagag cggcaagtac agcagcaact gtagagcgag tgatcgtttt tgatgccttc
     7021 tcattaggct tagttaattg aatggaagta acttcttcat cgttagtcaa tgtgactggt
     7081 tgaagaatga caaaatctaa gttagttcgt ttcagccatt catctgcata atgtttagta
     7141 atataataat ctgtcatgga ctcaggccat tttgctggtt catctgcata aacagcactg
     7201 accatcagat agcgagagac atgcgcattt tcagctgcga tcactgtttt gattgcgcca
     7261 tccaagtcta cttgcagaag attctttcct tgagagccgg cagcaaaaat caatacatcg
     7321 atgcctttga atgcttcggt cattttttcc acggaccaag ttaaatcaaa agagacatac
     7381 gaaacattct ctttttttac taatgtttgg gaagcaacat ctctcacccc tgcaaaaatt
     7441 tcatgggaac ttggagctaa atcttttatc aggtgtcggc caatttgtcc gtttgcacct
     7501 acaacaaaaa ttttcatttg gatcatcctt tcgacgtcaa atctttccat tcaatagtaa
     7561 caccaaaaga gaaaaaaatc agagatcggc tacgatgact atcagttagt aagagaaggg
     7621 tgcttgaagt atcttaacaa gacattgcat actgctgcag tcgcaagacc tgcgacgatt
     7681 ccttcaggta taccacttgt aaaaataacc gctagaatag ctgaataaac agcatttaaa
     7741 tttgttccga tgactttggc ataatcttgc tggaataaaa agtagatcat attcatcact
     7801 aaaatcgtat tggtcaatga accggaaagt ccagctaaga ataaagaaac ggaatctgct
     7861 ttttgtttca ttagtttgcg cagaccttta taaacgaaat aaggaaccac cccaatcaag
     7921 atgcgaggaa taaacgcgat cagcaaggct ttccagcttc catgttctgt tccgatgact
     7981 gggataaacg gagaaaagac aaaagacaaa ggtgtttgga taactgtgct acggatcatg
     8041 ctgatcagtc caaatgtacc gccaagaaaa gcacctagtc ttggtcctaa aacgatcgaa
     8101 gcaatgatta caggaatatg catggttgtt gcattgattg gaccgatagg gataaaacct
     8161 aaaggcgtaa cagctaataa aatcaaaata gctaaaaaca tcgcagttaa tgtaaagttt
     8221 tttgtatttt tcataagtac tccttttttc taagcatttc tttcattaag agtcagcatg
     8281 acctgttcga tgatcgtctg aatatcagct aaggcacctt ttccaaaatc tccacaagct
     8341 aaaagagatt ctcttggttc aatttcctga tagccgacag atctcaatgt ttccagatta
     8401 cgctgagtga tcgggttttg atacatattc gtgttcatgg caggggcaat caattttggt
     8461 gttcccactg gaagcgccat tgcgactgtt gaaagaagat cgtctgcaat gccgtttgct
     8521 agcttgccga taatgtttgc tgtagcaggc gcgatgagaa acaagtcagc ttgttttgct
     8581 agttcgatat gattgattac ctgcggattt gattcttgca tgacgtctgt atggactgcg
     8641 cgcttcgaca gagattgcaa ggttaatggc gtgataaaat gtgtgctgct tttagacata
     8701 atcacatcta cctgggcacc taatttgacg aattgacttg taagatcagc gcttttgtaa
     8761 gcagaaatac ttccagtaac gcctagtaag atctttttca ttttgattcc tccagtctaa
     8821 ttttctcagt aatcaatgca gcaatctcag atttactttg agtttcttcc actgttccat
     8881 ctttagataa gagatagccg tggtgtgtct cgccatggat ttccgtcaaa tcatttgcga
     8941 aaataaaatc agttttgtta tttatcagac aattttttgc gactgccaga agagattcct
     9001 ttggtacatc tactaatagt ttgaaaccaa tgatttttgc ttttggctgc cattcacgta
     9061 gataagaaat gatttttggt gttttcttca aaacgagcac aagatgctcg gtgttagaag
     9121 agatctttga aacagtggta tctgtctgtt cattttccgc aaaccaattg tccagttctt
     9181 cttgcgttga agaagtgggt aggttttttg ccagctgttc ttctgaaaaa ctaaaagcag
     9241 gagtaaaatc actgactgcc atactgtgga taatcgcatc gtagtgttct tttttcgtta
     9301 gttcttctaa ttgtaaaaat aagtccagtg cagattcgat cgtataaatc gttatattgg
     9361 agtgtctttt cggtttcagt gcttgtttcg tagtcacgta atcgattgtg gtatccggat
     9421 tggcagctaa ataatcagct aatgcttgcc ctaatcgtcc tgtcgaatga tttgtaatag
     9481 cacgtacttg atcgattttt tcagtcgttc ctccagctgt gattagaatc ttcatttatg
     9541 gtcacctaca tttactagtg ttcttccttg gtgtgttcca gcaagtagtt tagatgctgt
     9601 ttcaggcaat tgatccagag taatctcttg gacgaccagt tcatctgcaa tctgcaaatc
     9661 cgccatctgt tgccataaag aaagacgttg atctattggt acattgacag aatcaattcc
     9721 aatcaactgg atgtttcgca gaataaaagg caagacagtc gcgtttagtt tgattccccc
     9781 agcattgcca cataaagcta ctgcaccgtt ataagagatc aatggaagaa gactgcttag
     9841 ctgctcgcca cctactgtgt cgatgacata gtcgatttgc tgttttccta aagcttttgt
     9901 tgtctctggc agaaattcat caggtcgtac gacttgcaaa gcacctagac tttttagcca
     9961 tgtgacagac tcttcttttc tggaaaaggc aatgattgac tggaatccta gtttatgtaa
    10021 cagggctatc gctgtactgc ctacaccgcc agaagcacca gtaacgacaa cttttttatc
    10081 ggaggtcata ccttgacgga ttaaagcgtt cacacacaac atagcagtaa accctgctgt
    10141 accaaggatc atcgcttgac gcagcgtcat attttttggt agaggaacta accagtctcc
    10201 tggtactttt tgatattggc tataaccgcc tggatgggtg acgccaagcc caaaaccagt
    10261 agcaagtaca agatcttctt ttgaaaaacg tggattgtta ctttggacga cagttccgga
    10321 taaatcaatc cctggagtca tcggatatgt tcgtatcacc tgaccagact cgctcattgc
    10381 caatgcatct ttataattga catcagaata ggcaattttt accaagactt cattttcagc
    10441 caactcagta aatggcagat cactgattgc tgtatggaaa acaggttcct ttattacctg
    10501 aaaagatcga aaagcgttca ttcgtgagac actcctttta aaattttctt ttatcatacc
    10561 ataaagccta ctcttttatt cttgttcttc cgtaaattta gacaaaaatt aggtgaaaaa
    10621 taaagaatca aacaactaga atttattcca aaagataaag aaatattcag caaaaaaaat
    10681 tttacaaagg gttgcctttt tcaataaatt tcgtatacta atacgagtgg tagtaaacag
    10741 gaagtttact attagaaaac ttcgattcgg aggaaattat ggaaattggt gagaagttaa
    10801 gaaatttgcg catccaaaag aatttgacac aagaagaact aggcgaacgc acagatttgt
    10861 caaaagggta tatttcccag ttggaacgag atttaagttc accatcaatg gaaacattct
    10921 tttctattct tgaagtctta ggtgtgactc cagaagaatt ttttcatcaa gaagcagcga
    10981 atctgcaagt ggtttattca aaagaagatc atacggttta tttcgatgaa gaaaacgggt
    11041 atgaattgga atggctcgtg gctgattcga atgaaaaaga aatggagcct gtcttgctga
    11101 cttttgaagg tgcaggagaa tataagacgt ttgaaccttc attatctgaa acgtttattt
    11161 ttgtattaga aggggaatta gagttaagct taggagagac ggtctatgtg gcaaaagcag
    11221 gacagtcgat ctattatcaa gcgacaaaac aacatcaatt aaggaatcat ctcaaaaaac
    11281 gaacaaaagt attgattgtt gcaacgcaat catatttgta atataggggg taaaaacgtg
    11341 gggaaaccaa tcatttcatt tgatcaagta gtgaaacgct atgatgatga aacagtatta
    11401 aaaaaagtaa gttttgagat cgaacaagga aaattttata cactgcttgg tccttccggc
    11461 tgtggtaaaa caacgatcct acgtattata gctggattca ccgaagctac agaaggggac
    11521 atctattttg aagggaaacg gttgaatgat ttgccagcta ataagcgaca agtaaataca
    11581 gtcttccaag attatgcact ttttcctcat atgaatgttt ttgataacgt tgcttttgga
    11641 ttgaagatca agaaaatgtc caaatcagat atcgaaaaga aggtaaaaga tgctttgcgt
    11701 atggtccagc ttcctggata cgaaaaacgg gagattagtg aaatgtctgg tggtcaaaga
    11761 cagcgtgtag cgattgcacg agcaattgta aatgaaccga aagtattgct attagatgaa
    11821 ccgttatcgg cgcttgattt gaaactacgt acagatatgc agtatgaact gcgggatctg
    11881 caacaacgat taggaatcac gtttattttt gttactcatg accaagaaga agcgttggca
    11941 atgagtgatg agatttttgt catgaataaa gggaaaatcg tccaaagtgg tacacccgta
    12001 gatatttatg atgagccaat caatcatttc gttgctgatt ttgttgggga gagcaatatc
    12061 gtggacggtg taatgatcga agataatcta gttgagtttg ttggtaagca atttgaatgt
    12121 gtcgatggcg ggatgcgtct aaatgaacca gttgaagttg ttttacgccc agaggatttg
    12181 acgatcacga caccagataa aggaaaacta gtcgtaacgg tcgatactca gctgtttcgt
    12241 ggtgtccatt atgaaatcat ctgttatgat gaacagcaaa atgaatggat ggtccattca
    12301 acgaaaaaag ctaaagaagg aagcaaagta ggattggcct ttgagccaga agatatccat
    12361 gtcatgcgtt tcaacgagtc tgaagaagaa ttcgatgcac gtcttgacag ctacgaagaa
    12421 taggggggac aagaatgaaa acaatgcgca ggatctattc cgtcccttat attttgtggc
    12481 tcggtttatt cgtgatcgcg cctgtattaa tgatcattta tcaatctttt tttgatatga
    12541 atgggcaatt cacgctgaat aattatctat cttattttac gtcggggaca tcgctttcta
    12601 tgacgatcaa ttctgtatgg tatgcatttt tgatcacgtt tttcacgctg atcatcagtt
    12661 acccgactgc ttatttttta acaaaactga agcacaagca attatggctg atgctgatta
    12721 ttttgcctac atgggtgaat ctgctgctta aagcttacgc atttattggg attttcagtg
    12781 tgcacggcag cgtgaatcat ttctttgatt ttatcgggat cgggccgaaa cagatattat
    12841 tcacagactt cagttttctg ttcgtggcaa cgtatatcga gattccattt atgattatgc
    12901 caattttcaa tgcattggaa gagatgaatc cttctttgat cagtgctagt agagatttag
    12961 gcgcgaatag ctttgagacc ttccgacgag tgatttttcc gttatcatta aatggtgtga
    13021 aaagtggtgt ccaagcagta tttattcctt ctctttcttt attcatgctg actcgtctga
    13081 ttggcggaaa tcgggtgatc actttaggaa cggctatcga acagcatttc ttggttacac
    13141 aaaactgggg gatgggttct acaatcggtg tgattttaat cattgctatg ttcatcgtga
    13201 tgctcctgac aggcgagaag aaaaaaggag gtcgctcata aatgaaaaaa tttaaatggt
    13261 cgtatcttta tttagggatc gtctttttat tcttatatgc accgatcttc tatctgattt
    13321 tttattcctt caatgaagga ggaacgatga acagctttac aggatttact tgggaaaatt
    13381 atgaagctgt ttttgaagat acacgtttga tcattatcgt attgaatacg ttcatgctgg
    13441 ctttcttatc tgcattgatc gccacgatca tcgggacatt tggggctatg ggaatctatt
    13501 acacgaaaaa acgacgtgca cggacagcgt tgttgagcat gaataacatc ttattagtct
    13561 ctcctgatgt tattatcggt gctagtttct tgattttctt tacactacta ggattcggat
    13621 taggttttca gagtgtattg ctttcccata ttgctttcag cattccaatc gtcgtcttga
    13681 tggtcttgcc aaaattgcag gaaatgaacg attcaatggt cgatgcggct agagaccttg
    13741 gtgctaataa tttccaagta gtcaaaaata tcattttgcc ttatctttct ccaggaatca
    13801 tagctggata ttttatggca tttacctatt ctcttgatga ttttgcggtt acatttttcg
    13861 tcacaggaaa tggctttacg acgctctcag tagagatcta ttctcgtgca agacaaggaa
    13921 tcagtttaga aatcaatgcg ctaagtgcgt tagttttctt gttctccatg atattggtca
    13981 ttggatacta ctttattagt aaagaaaacc gtccaaaacg gctgaagaaa aacagaagag
    14041 tacgcgaggg ggtggcacgg ctgcgatgaa aaaattacaa tctctgtttt taggtattct
    14101 catcattatt gcggtgctgc tgcttggtgt tcgtcaactt gaaaaaacaa gtggcatgag
    14161 tggagcaaag gtacttacca tctataactg gggagattat atcgatccta gtttgatcac
    14221 gaaatttgaa aaagaatatg gatacaaagt gaattatgaa acatttgatt ctaatgaagc
    14281 gatgttcaca aaaatccagc aaggtgggac aaactacgac atcgctatac caagtgaata
    14341 tatgatccaa aaaatgatca aagaaaaact agtacttcca ttagatcatt cgaagatcaa
    14401 aggattaaat aatattgatc cacgattttt agacttggat tttgatccag ataatcagta
    14461 ttcgattcca tatttttggg gaactttagg gatcgtatat aacgataaat tctttgatgc
    14521 aaatcaaatt aagcattggg atgatttgtg gaagccggaa ttaaaagaca gtttgatgct
    14581 gattgacggt gcacgagaag ttatggggct ttctttgaat agtttaggct attctttgaa
    14641 tagtaagaat atggcacagc tgacagaagc agcaaataaa ttgaatcatt taacaccaaa
    14701 tgtcaaagca atcgtggcag atgagatcaa aatgtatatg atcaatgaag aagcctccgt
    14761 ggcagtgaca ttttctggcg aagctgccga tatgatgtgg gaaaatgaac atttacatta
    14821 tgtgatccca ccagaaggat ccaatctttg gtttgataat atcgttatgc caaaaaccgt
    14881 gaaaaataaa gaaggtgcct atgatttcat caactttatg ctagaaccgg aaaatgcggc
    14941 acaaaatgca gagtatattg gttattctac accaaatgaa gaagcaaaag cgttattgcc
    15001 aaaagacatt tcgtcggacg aacaattcta tccatctgat gacacgatct cccatcttga
    15061 agtctacaaa gatctaggat cgaaatacct aggcatctat aatgacctat tcttagaatt
    15121 caagatgtat aggagataat ggcaaaactc agtaagaatg gggacttatc aacgttctaa
    15181 agagctaaaa atcgtccttt ttctatctga gttttccaat cttctaccaa aagcggatga
    15241 aacgccgctt tttttgtgtt attttatttt ttctaccacg tttttctacc agagaaaagt
    15301 ggacaaaacc attgtaaaat agggcccgag acgggctagt aaatctaaga ttgaaaaagt
    15361 tacagaagga taaagtaatt ggaaactatg caaat
//
LOCUS       DAGCYL010000054        15266 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.055, whole genome shotgun sequence.
ACCESSION   DAGCYL010000054 DAGCYL010000000
VERSION     DAGCYL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.055"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            17..1135
                     /locus_tag="MB216_001292"
     CDS             17..1135
                     /locus_tag="MB216_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4526137.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1263..2639
                     /locus_tag="MB216_001293"
     CDS             1263..2639
                     /locus_tag="MB216_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBT4526138.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3040..4620
                     /locus_tag="MB216_001294"
     CDS             3040..4620
                     /locus_tag="MB216_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBT4526139.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4818..5042
                     /locus_tag="MB216_001295"
     CDS             4818..5042
                     /locus_tag="MB216_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBT4526140.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDLKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(5247..5567)
                     /locus_tag="MB216_001296"
     CDS             complement(5247..5567)
                     /locus_tag="MB216_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBT4526141.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(5715..7949)
                     /locus_tag="MB216_001297"
     CDS             complement(5715..7949)
                     /locus_tag="MB216_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4526142.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8428..8694
                     /locus_tag="MB216_001298"
     CDS             8428..8694
                     /locus_tag="MB216_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBT4526143.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            8694..10421
                     /gene="ptsP"
                     /locus_tag="MB216_001299"
     CDS             8694..10421
                     /gene="ptsP"
                     /locus_tag="MB216_001299"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBT4526144.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            10585..11631
                     /locus_tag="MB216_001300"
     CDS             10585..11631
                     /locus_tag="MB216_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBT4526145.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            11648..12871
                     /locus_tag="MB216_001301"
     CDS             11648..12871
                     /locus_tag="MB216_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBT4526146.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            12960..13847
                     /locus_tag="MB216_001302"
     CDS             12960..13847
                     /locus_tag="MB216_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBT4526147.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            13933..14163
                     /locus_tag="MB216_001303"
     CDS             13933..14163
                     /locus_tag="MB216_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBT4526148.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(14220..15185)
                     /locus_tag="MB216_001304"
     CDS             complement(14220..15185)
                     /locus_tag="MB216_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBT4526149.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRAQLPDLQ
                     EMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMIASADVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNKKEAEKQLN"
BASE COUNT         4953 a         2621 c         3115 g         4577 t
ORIGIN      
        1 gaaaggagga aatctattgt ttgaaaaact tagacagtct aaactaatgt tttggtcgct
       61 tgaattattg atcatagctg cattgatcat gatttcatca gaaataaact ttattttcga
      121 accgatcggg acattttttt ccacgttatt tgcacctgta ctgattgcag gctttcttta
      181 ctatttattg aatcctattg tcaagctgtt gatgaaagca aaaatcaaaa gaatatgggc
      241 tgtcgcaatt gtgctgctcc ttttagttgc agcaattatt tggattttac ttagtgtcat
      301 cccaagtcta gtacagcaaa tttcctcatt ggcatctaat atgcctgatt tcatcaaaca
      361 ggtagaaagc tggttaaaag aggtagccga attgcctttg ttcaaggaag tagatatcaa
      421 taaatatttt gagcaattag acatttctta cgggacaatc attcagcaat tccttagtgg
      481 agtatccaat agtttaggtt cgatcgttgg aacgatcgct tcggcaaccg tagtaatcat
      541 tacagcacca ttcatcttgt tctatatgtt gaaagacgga gaaaaactag tgccaaacat
      601 ccaacagttt ttcccagaaa aaagaagaaa ccaaattgtg gagttattgg ggcaactgaa
      661 taaaacattg gctaattaca ttagcggaca agcaatcgaa tgtttattcg ttggtacctt
      721 cacattttta ggatatatgg taattggtgt ggattatgct ttcttatttg gggtcattgc
      781 gggattgaca aacctaattc catatttagg accttatcta ggactagcac ctgcatttct
      841 agtaacagtc ttcaatgatc cggtaaaagc attactttgt tgtgtggttg tgctaatcgt
      901 ccaacagttg gatgggaata tcatttatcc taatgtaatt gggaaatcac tgaaaatcca
      961 tccattaacg atcattatcg ttcttctagt agctggaaat attgccggat tgcttggtat
     1021 cttcttaggt gttccttttt acgcaatctg caagacgatc atttcctata taatcaaaat
     1081 cgtcaaagaa gacaagcaaa atgaaaacaa gaagaaaatt gctacgacct cgtaggtagt
     1141 tttagtacac ttgtttttat tgcaaagaaa attgctttgt gataccattt taacgtggct
     1201 atagtgccac gtttttttct atcgtaaact aaaaattaat tttataagga gagaatgcga
     1261 ttatgaatgc tgaccctgag agtcagtcgc tgttagcgca gattttatta ttgatcgttt
     1321 tgacattgat caatgctttt ttagcagctt ccgaaattgc tgttgtttcg atcaataaga
     1381 atcgaatcga gcaaaaagct gatgaaggag atgtgaaatc tcaaaagctt ttaaaaatat
     1441 tgcagaatcc aaataatttc ctatcgacaa tccaagtagg aatcactttg gtcaacattt
     1501 tgtctggtgc ttcactagct aatactttat cggaacggtt agctccggta ttaggtggcg
     1561 gcacagcggc aaaaaatatt gcaaatatca ttgtgctggc tattttgacc tatgtatcga
     1621 ttgtttttgg agaattgtac cctaaacgta ttgctttaaa taaatcagaa gaagtcgcaa
     1681 agttcacttc agggctgata cgcttgattg gtgtagtagc taagcccttc gtctggctgc
     1741 tttctgcttc cacaagttta ttggcgcgta tcacgccgat gacttttgat gatgaagatt
     1801 caaaaatgac cagagatgaa atgcgttata tgcttgaaaa cgaaggcgta ttgaataatg
     1861 aagaattaga aatgctccaa ggggtttttt ctcttgatac gaaagttgcc cgagaagtca
     1921 tggtcccacg aacggatgca tttatgattg atatcaacga cactgtggaa gaaaatgtga
     1981 atgaagtttt atccgaaaac tattcaagaa tccctgttta taacgaagac aaagataaag
     2041 tagtcggaat ccttcataca aagaatttac taaaagctgc ccataaattc ggttttgata
     2101 atttagatat caagaaaatc atgcaggagc ctttattcgt tcctgaaacg gtctttattg
     2161 atgatctgtt atatgaaatg aaaaagacgc agaatcagat ggctatttta ttagatgaat
     2221 atggcggcgt cgttgggtta gcaacactag aagacctttt agaagaaatc gtcggagaaa
     2281 tcgatgatga atcagatgaa gttgagaatc tttatgaaaa aatcgatgaa cacgaatata
     2341 tcatccaagg acgtatgctg attgatgagt tcaatgaagc ttttgacagt gatctgcata
     2401 tgagtgatgt agatacgatg gctggttatt tgattacagc attaggcatg attccggatg
     2461 aaggcgaaaa gttatcgttt gatgtcgata atattacgct ggtttctgaa gaaatggaag
     2521 gttctcgaat ccttaaaatc cgtgtcattt ttcatgatcc ggaagaaaca gaagcggaac
     2581 ctgaagaaga acgaagatac ttcagaaaag aattcgaaga tgacgagcca agaagataat
     2641 tggaaacttg gttatgaaag acgtgttgct tgaataaaca agcaacacgt cttttctttt
     2701 agtcagctga caaaaaaata tttgactctt atggtttggt ggctttttca ttcgatgaat
     2761 ccatgctata ctgattcagt tgtagtagtt gacaaaggtt gtgaaaaagc tatcctgcat
     2821 taagtaataa gagcagccaa gcaaaaacag cttttcgtat ttttggcttg ggtgggctta
     2881 ttgctgatag gctgtctcta gaacgccgtt tactcggaaa aagagactgc tacacggctt
     2941 ttgtcacagc cctatatgaa attgtctttc tttaagagat cggacaatct gtgcctagag
     3001 aaaatataaa taattgaatg gaagaaatgg agttaggtta tgaataatcc agaattaaag
     3061 caacaagtgg acaatcgcag aacttttgcg atcatttccc atccggatgc tgggaaaaca
     3121 acgattacag aacaattact attgtttggt ggggctatcc gtcaagccgg gaccgtaaaa
     3181 ggcaaaaaaa caggtaattt tgccaaatca gactggatgg aaatcgagaa acaacgtggg
     3241 atttctgtta ctagttctgt tatgcagttt gattatcaag ggaaacgagt aaatatcttg
     3301 gatacaccag gacatgaaga tttttctgaa gatacttatc gtactttgat ggccgtggat
     3361 agtgcggtca tggtaatcga tagtgcaaaa gggatcgaag cacagacaaa gaaattattc
     3421 caagttgtaa aaaaacgtgg catcccgata tttactttta tcaacaaact agatcgtgat
     3481 ggtcgtgaac cattggaatt gttagaagaa ttagaagagc tattagatat cgaatcttat
     3541 ccgatgaatt ggccaatcgg tatgggtaaa gggctagaag gtctttatga tatctaccat
     3601 aatcgtgtag agttttatcg tccggaaaat tatcaggatg aacgattagt agaattggat
     3661 gaagatggac tgcttcccga aaatcatcct ctaactgaaa attccttata tgaacaagtc
     3721 ttagaagaag tagaactaat caaagaggct ggagatgcat ttaatcaaga aaaaattgct
     3781 cgtggcgatc agacaccagt attttttggt tctgcattga ctaacttcgg tgtacagact
     3841 tttttagaga ctttcgttga attggcacca gctccttatg gacacaaaac acaagatgat
     3901 cagatggtaa gtccttatga ggaagaattt tcaggtttcg tattcaagat ccaagcaaat
     3961 atgaatccag cccatcgtga ccgaatcgct tttgtccgaa tctgttctgg aacattcgaa
     4021 cgaggaatgg atgtctggtt ggaacgtaca aataaaaaac tgaaattaag caatgtcaca
     4081 caatttatgg cagattcgag agaaaacgtc gaaaaagcag tagcaggcga tatcatcggt
     4141 gtatatgata caggcaatta tcagattggg gatacattgt tcgaaggaaa actaaaagtt
     4201 gcttatgaag aactcccttc atttactcca gagctgttta tgaaagtgac tgcaaaaaat
     4261 gtcatgaaac aaaaatcctt ccacaaagga atctatcaat tagttcaaga aggagcgatt
     4321 cagctttata aaacatatct aacggaagaa tatatcattg gagctgttgg tcagctacaa
     4381 ttcgaagtat tccaatatcg tatgttaaat gagtataacg ccgaagtaat catgtctcca
     4441 atgggacaca aaatcgctcg ctggatcgat ccagaagatt tggatgagaa gatgagttca
     4501 agtcgcaata tcttggctcg tgaccgtttc gaccaaccat tgttcttatt cgagaaccaa
     4561 tttgcagaac gctggtttgc ggataaatat cctaatgtaa aattaaaaag tttgatgtaa
     4621 agaagtcttg acacaagctg ccctgcatca agtaataaga atagccaaac aaaaacagtc
     4681 tctcatattt tgtttagagc cgtgacggat tttccgtgac ggctcttttt actatattaa
     4741 aatcatagaa atgtctaatc aaaaaaatat aaaaattatt ttgcgtacat atgattaaag
     4801 aaaggagagt gtgacacatg gaaacagaat ggcaaatacg tgaaagaaaa aaacagaaaa
     4861 aagaagagat gttacgatta tgtcgattat tagatttaaa agactgggac aaaaacgagc
     4921 aaattgcaca aaaagtacga tcaatcatcc ataaagagga aaaagttaaa aataaagaca
     4981 cgcgtagaac tgaaaatgaa aagagaaaag tacaaaagaa aagagaaaaa tcaaaaagct
     5041 agaaagaaaa agaggctgag acagaagcgt ttaactccaa gaaataagct ggaattcaca
     5101 aaaatttgaa aagcaatttt cgtgaatttt agcttatttc cgaaggagtt gcttctgctt
     5161 ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt ctttactttt gtcccacgct
     5221 ctttaattat tatgttcaaa atttgtttac acggctaatg tagcgcctaa ttcatgtttt
     5281 ggaatcgaga tttcaacaag gcctgccaat tcgattactt tgatttcatc ctcgtggtaa
     5341 acggcgtctt tgattttatc taagaaataa tttttagcat cttcgatttc tttcggctgg
     5401 attttacgtt tgtcattgat cagcagaatt tcattatgtt caggggcttc agtgtagaca
     5461 atagtggtat ttccggctgt gtagaccttg actagatggg catcagtgtt ttcaagctgg
     5521 tttaatacaa gacgggaatg gctgtttgtt acatttacca atttcatggc gctcacctct
     5581 tttatgtatt tttaattgct tgtttctctt attataattt cttttttata aaatgaacag
     5641 tattttgact tttctgaaaa aagaatgaga cggcatttct caaagaaata ccgtctcacg
     5701 ctataaaact ggatttattc agccgtttct tcggctgatt ctttagccaa acgttctggt
     5761 ttagaagtaa cgatgatttt tccatcttta tccagttttg ctttcaactc atggatagaa
     5821 gggtgatcaa gataaaattc agcgatacca tcttcgattt gttcttggat cgtgcgtcga
     5881 agaggacgag cacccataga tggatcatag cctaagtcaa ctaatttttc tttgacattc
     5941 gtaggaactt cgatgtgaag ttgctgtgct gccagcattt ggttgacatc gtctagcatt
     6001 agtgtgacga ttgtcatcaa gttttctttt gataatgcac tgaattcgat gatcccgtca
     6061 aaacggttca gaaattctgg tgtaaagtag ttgtttaatt ggttcaatac agaacgagta
     6121 acaccttcgc gtgccgcacc aaaaccaacg tttgcttcta ctttaccagt tccggcatta
     6181 cttgtcataa tgatgatcgt atctttaaag ctgaccgttc ttccttgtgc atctgtcaat
     6241 cgaccatcat ctaagatttg caagaacata tgcaaaacat ctggatgagc tttttctact
     6301 tcatctaaca agacaagact gtaaggattt ctccgtactt tttctgttaa ttgaccagct
     6361 tcttcgtatc cgacataacc tggaggagaa ccaatcaatt tagagacact atgtttttcc
     6421 atatattcag acatatcgaa acgaatcatt gaatcttgtg aaccgaatag ttcgtaggca
     6481 agttgtttcg caagctctgt tttacctaca ccagttggtc caacaaataa gaaggagccg
     6541 attggacgat tctgtttgtt taatccaaca cggtttcggc gaatcgcttt cgctactcga
     6601 tctacagcgt tatcttgtcc gatgacatga gctttcaagt catctgctaa gtttttcagt
     6661 tgtgtctgtt ctttttcttt taattcaccc actggaatac ctgtccgttg ttcaacgatt
     6721 ttttccatat ctttttcggt aatcacaggt gtttcctctt cagtgagctg acgttctttc
     6781 attttttgaa gtttattgat ctgatcgcga taataagctg ctttttcgaa atcttcttct
     6841 cgagaagcta aaactttttg ttcttctgct tcttgcagtt ttttctcgat tgttttagga
     6901 tcaacgattt ggatcgtcaa gtttttctta gaacctgttt catctagcaa atcgatggct
     6961 ttatccggca agaagcgatc ttggacgtaa cgattggata aagttgcggc tgcttcgatg
     7021 gcctcatcag tatattttac atgatgatag tcttcataac gtttttgcaa acctttcaaa
     7081 atactgatcg tttcttcgac agttggttca tctacccgta caggttgcat acgacgttct
     7141 aaagcagcgt ctttttcgat gatgcggtac tcatttaagg tagtagcacc aaccatttgc
     7201 aattctcctc gagctaaagc tggttttaaa atattaccag catccatatt tccatcacca
     7261 gcagaaccag caccgacgat ttcgtgaact tcatctatga ataggatcac attttctgct
     7321 tgacggatct cttcaatcaa tttttgcata cgttcttcaa attgtcctcg tataccagta
     7381 ccttggacta aagatacgac atctaaacga atgacttctt tgtccattaa tttttgcggt
     7441 acatcaccat ctacgatttt ttgtgctaaa ccttcaacaa ctgctgtttt accaacacct
     7501 ggttctccaa tcaagacagg attgttcttg gttcggcgat ttaaaatttc aatgactcgt
     7561 ttgatttcgt catcgcgtcc gatcacagga tcgatatcac cttgtcgtgc ttgttctgtg
     7621 atattgattc cgtattctcc caataaacct tctgtattgc cttgaggtgg ctgaccacca
     7681 ttgaagttgt tgtttccgcc aaattgtgtt ggtggtgttt gctcataatt tccttgctgt
     7741 tgcatttggc gagacatgga acggaaaaga tcgtccaaac tgccaaatcc aaatggatca
     7801 ttttgggcca tattagtcag acctccattt tgttgatttt tgattttttg ataacagctc
     7861 tgacagtaat cgagttgtgt ccgctgacca ttgacagtgg catacaagtg aatcgttgcc
     7921 tcattcttgc cgcagttttg acaaagcatg aatcattcac gtcctttcac aaaaatgggt
     7981 acatccatat tttacatctc gtttttcatg aggtaaaaga ataaccccta gttttttaag
     8041 agatttatga ggtagaaaag gtgaaatctc tattgaccat tcctgacctt tgactcttat
     8101 tatactaaaa ataaaaaggg aagcaagtaa ttcgtttcat atgaaaacaa agagggtcaa
     8161 aggaaagaat gataaacgtc aggagaagag aaagcgacaa gaaaaagaaa ataaacacgt
     8221 ttgttataaa atgtgaagaa aaggatgaaa aagaatccgg aataatttga atacttttat
     8281 ctgaaattga atgaaaactg ttgtatctaa cgaaaaaaaa tgttaatctt tacagatgaa
     8341 agggtggcat ttgttaggat tctctgaatt actaaaaaag aaaaccctca cataaaaaca
     8401 acactcactt aaaggagacc gattaatatg gaaaagaaag aatttcacgt agtagcagaa
     8461 actgggatcc acgctcgtcc agctacacta ttagtacaaa cagcaagcaa atttaactct
     8521 gatgtaaact tagagtataa aggtaaatca gtaaacttga aatcaatcat gggcgttatg
     8581 tctttaggtg taggtcaagg ttctgatgtt actattactg cagaaggtgc tgacgaagct
     8641 gatgcaatgg cagcaatcgt tgaaacaatg aagaaagaag gcttatctga ataatggttg
     8701 aaatgctaaa agggattgcc gctagtgacg gtgtagccgt tgcaaaagct tacctgctag
     8761 ttcaaccgga tttaacattc agtaaggcaa cagtagaaga tactgcggct gaagaagctc
     8821 gtttggatgc tgcattagct aaatctactg aagaattgca gcaaattcgt gaaaaagcag
     8881 cgcaaagctt aggtgaagca gaagcgcaag tatttgatgc ccatttgatg gttctttcag
     8941 atccagaaat ggtaggtcaa atcaagcaaa atattaaaga caacagcgta aatgctgaat
     9001 cagcgctaaa agaagtaacg gatatgtata tcggtatgtt cgaagcaatg gaagataatg
     9061 cttatatgca agagcgtgct gcagatattc gtgatgttgc taaacgtatt ttagcgcact
     9121 tattaggcgt tactttacca aatccatcaa tgatcaacga agaagtggtc gttgttgcac
     9181 atgacttgac tccaagtgat acagcacaat tagatcgcaa ttttgtaaaa gcgtttgtaa
     9241 cagacattgg tggacgtact tcacattctg ctatcatggc acgttcttta gaaattccag
     9301 caatcgttgg tacaaaagaa attactgcta aagtcaaaga aggcgatatt ttggctgtca
     9361 acgggatcga aggcgatgta atcatcgatc ctacagatga acaaaaagct gaatttgaaa
     9421 aagcaggtgc agattacgca gcacaaaaag cagaatggga aaaattaaaa aatgcagaaa
     9481 ctgtcacagc tgacggcaag cattttgaat tagctgcaaa catcggaaca ccaaaagatc
     9541 tagttggtgt ccataataat ggtggagaag cagttggtct gtaccgtact gaattcctgt
     9601 atatggattc tccagacttc ccaacagaag atgaccaata tgaagcttac aaagctgtat
     9661 tagaaggtat ggaaggaaaa cctgtagttg ttcgtacaat ggacatcggt ggagataaag
     9721 aactaccata tctgcaatta cctcatgaaa tgaatccatt cttaggatat cgtgcattgc
     9781 gtatcagttt atctgaacaa ggcgacgaaa tgttccgtac acaaatgcgt gcattgcttc
     9841 gtgcatctgt tcatggtaac ttacgtatca tgttccctat ggtagctaca ctcaaagaat
     9901 tccgcgcagc aaaagcgatc tttgaagaag aaaaacaaaa attgatcagt gaaggcaagg
     9961 aagtatctga tacgatccaa gtcggcatca tgatcgagat tccagcagca gcagtcttag
    10021 cagacaagtt tgcaaaagaa gtagatttct tctctgtagg tacgaacgat ttgattcaat
    10081 acacaatggc agcagaccgt atgaacgaac gcgtttctta cttgtaccaa ccatataacc
    10141 catcaatttt acgtttgatc aaaaatgtaa ttgatgcagc acacgctgaa ggaaaatggg
    10201 caggtatgtg tggagaaatg gctggagatc aaacagctgt tccattgcta gtaggtatgg
    10261 gtctagatga gttctcaatg agtgcgacat ctattcttaa aacgcgtagt ttaatgaaac
    10321 gcttggacac agcgaaaatg gctgaacttg ctgatcgtgc attgaaagaa tgcgatacga
    10381 tggaagaagt cgttgaactt gttcatgaat atgtaaaata aacaacgtat caaaagctgg
    10441 tgtggcttgt aaaagagcta cgccagtttt tttgttcttt tttaagaaaa cgtttttgtc
    10501 agtaagaata gataaaaata gggcttaagt ttgagtgaca gtttgtaggc tatgttatac
    10561 tatataagtg aagggggacg taaggtgaag gtattattat attttgaaag tgaaaagatg
    10621 cttgcaaaat ctggaatcgg gcgtgcgctt gatcatcaaa aacgtgctct gacggaagtc
    10681 ggtatctctt atacactaga tgaaaaagaa gattatgaca tcctgcacat caatacatac
    10741 ggaatcaata gtcataatat ggtcaataaa gccagaagag aaggaaaaaa agttgtctat
    10801 catgcgcatt ctacagagga agatttccga aattccttta tcggatcgaa ccagctttct
    10861 cctatcgtaa aaaaatatct ggtaggattg taccaaaaag cggactatct gatcacgcca
    10921 acaccttatt caaaacagtt gttggaaagc tatagtatcc gtgtgccgat tcaagctatt
    10981 tccaatggga tcgacttaga gaaatatcgc cctgatccaa tgaaagaaca aaagttcaga
    11041 gaatatttca aactgtctcc ggatcaaaaa gtaatcattt gtgtcggttt gtttttcgaa
    11101 cgtaaaggaa tcattgattt cgtagaaatt gctaagaaga tgcccgaata cacatttatt
    11161 tggtttggtc atgttcccat gtactcgatt cctcggaaca tccggaaaat tgtaaaagaa
    11221 gatcatcctg aaaatgtcct gtttcctggt tacattcgtg gagagatcat agaaggtgcg
    11281 tattcaggtg cagatctatt ctttttccct tcttatgaag agacagaagg gatcgttgtt
    11341 ttagaagctt tagcaagcaa acaaaacgta cttgttcgag atattccagt gtataatggc
    11401 tggttagaag atagcaagaa ctgctatatg gggaagacaa acgaagaatt tatccgactg
    11461 atccaacaga ttaccaatca agaactaccg agtacgacac aagccggtta tcaaacagca
    11521 aaagagcgaa gtatcaataa aatcggtgaa gaacttaaaa atgtatacga aagtgtactg
    11581 aatgaaaaag tctctagcaa attaaaaaaa gtaaatcagg tcaaggacta gtttggaggc
    11641 gaaaaacatg aaaatcggct tttttaccga tacctacttt ccgcaagtca gcggagttgc
    11701 tacatcgatc aaaacattaa aacaagaatt agaaagacat gggcatgaag tctatatttt
    11761 tacaacgacc gatcctaatg cggataagtt ggaaaaagat gtgattcgaa tgccaagtgt
    11821 accatttatt tctttcaagg atcgaagagt tgttgttaga ggcatgtggt atgcttattt
    11881 ggttgccaaa gaactagaac tagatcttat tcatacccat acagaattcg gagcagggat
    11941 acttgggaag atggtagcta agaagttgaa aatacctctg atccatactt accatacgat
    12001 gtatgaagac taccttcatt atatcggtaa aggaaaagtg atcaggcaat cccatgtaaa
    12061 atacttgtct agattatttg caaatcatac gacaggtgta gtttgtccaa gtgaacgtgt
    12121 tattgataca ttacgtagct atggtgtaat agcaccgctt cgcgtgattc ctactggtat
    12181 cgacattgaa aaattcaaac gttctgatat cactcagcag gatatcagca aattaaggga
    12241 gtcactaggt cttcaggaaa accatttgat gattctttct ttaagcagga tttcttatga
    12301 aaaaaatatc caagccttga ttaatgggtt tcctcaggtc atctccgctt atcctaacgc
    12361 cagattagtt atcgtaggaa aaggacctta tgtagaagag ttagaggaat tgatctcaga
    12421 actgcagtta gaagaatttg tgcagttcac aggagaagtg gacaacaatg atgtagcact
    12481 ttattataaa gcggcagatt attttgtcag tgcttctact tcagaaacac aagggcttac
    12541 ttatacagaa gcgatggcag ccggaacacc ttgtgtcgtg gaaggaaatg cttatttgaa
    12601 ccgattgttc gatcatcctt cattagggaa aacttttgaa accgacgaag actttgcacc
    12661 agcacttatt gattatattt ccagtgaagt tcctaaagat ccaaaagttt tagaagctaa
    12721 actttatgat atttcatcta cacatttcgg cgaagctatg atcgaatttt atcaagacat
    12781 ggttgtttat catgaagaag agatgcgaag aaaagaagaa gaagactcga ttgaacgaat
    12841 caaaataaaa ttaaattcat ttaaaagata aaattcaaac attttttatg aaaaaatcct
    12901 tgtttctagc gtatactagc atagaagcaa ggatttttga tttttaggag gtcgaaaaaa
    12961 tgaaacttgg ttttatcgga acaggcgtca tgggaagtgc tgtcgcacgc catctactag
    13021 aagcaggaca cgaagtagct gtctataatc ggacaaaagc aaaagctgat cctttagtaa
    13081 cagaaggagc tatctgggca gatacaccaa aagcggtagc agaacaaagt aatattctct
    13141 ttacaatggt aggttacccc aaagatgtag aagaaatcta ctatggacag tcggggattt
    13201 tttctgcaga tatctcagga catattttag ttgacttgac tacaagtaca ccaagcctgg
    13261 cagaaaaaat tgcaaaaaca gcaaaagaaa aaggcgcaga tgcgttagat gcaccggtat
    13321 ccggtggtga cttaggagca aagaatggta cactgacgat catggtcggt ggagaagaag
    13381 ccgtttatga tcaagtactg cctttattca aagaatttgg tacgacattt acattgcatg
    13441 gttctgctgg gaaaggtcag catacaaaaa tggctaatca gatcatgatt gcaggtacga
    13501 tgacaggaat gacggaaatg cttgtctatg cacaaaaaaa cggacttgat ttaaagaaag
    13561 tgattgagac gctttcaggt ggaagtgcag ctaactggtc tttaagcaat tattctcctc
    13621 gtattttaaa agaagattac acacctggtt tttttgtcaa acattttatc aaagatttaa
    13681 agattgcatt agaagaagca gaaaaaatgg accttgtatt gccagcaact acccaagcat
    13741 tgaaactata tgaagagttg gcagataaag gttttgagaa tgacggaacc caagctttga
    13801 tcaaactttg gtggcctgaa ggaaaaatac cagaaaaaaa atcataaaaa acgagttttt
    13861 ttagagaaaa tcaatgcaag taggctatgc ttttgataca atataacaga aataagaaaa
    13921 ggaggacctc cgatgacaca ttcacaatta gtcgcaatca taaaacgact ggaggctatg
    13981 gtcgagtcag cagataatga actgcaagtt cgccgttttg aaaaagaagg cgtagagaag
    14041 tgtacagtaa cttatgataa agcgaccgat acatttgaat taactgaaac agatactcat
    14101 cagcaatacg aatttgataa tatcgatatc gtagcgatgg aaatctatga cttaatccaa
    14161 taaattttag aataattgac atttgaagtt gaagatgcct aaaagaagcc gagaagcaac
    14221 taatttagtt gcttttcagc ttcttttttg tttaacagtt caatttcaga ctgttctttt
    14281 tctaattttt tgcggtaact gatcaagatt tgtacaggat cgatttcttc ttttgacaat
    14341 tgacgtttca aagataaatg agcttctttc ttttcataat agaagtcagg agaaacgtca
    14401 gcagaggcaa tcattgtttc tgcaagttga ccataacaaa cgaggtagtg ctttttctga
    14461 ctctcatctg ctgctaatgg catgctgaag gtgagcgatg gtgttcttaa aaaatgcttt
    14521 tgttcctgga tccgtctaat aaaataagta actaattcga tatctttttg aagcataccg
    14581 gctttttcga aattcaactg atagatgaaa tgttgttgtc tttttttcaa gtaagttagt
    14641 agctttttat tttggccttg aaacagccct tggagttcct tttttttctg ttcaaaagtc
    14701 atttcctgta ggtcaggaag ctgggctcgc aaagctaaca aactaatttc agacaaccaa
    14761 ggcatttgat aagtttcttc cattatacgc aaaatagacg gcattttttt atagagacga
    14821 aaaggcccgt atgttcgagc cgtaggagta tctgttaata caaatccctt attggtaata
    14881 tgtacataat tataattact gaaataattc atttgtcggt tataaaaagg acggtaatgt
    14941 tggatcaatt gacactcgag caataaagca tctaattccg tgtctactac gacgaaatca
    15001 aaatctgtta tatgatgaat catacgtaat actttttttg aatgctgttt attctgatga
    15061 aaatagctgc ttactctatt tttgagccgt tttgctttac caacatagat gaccttacca
    15121 tcacggtctt tcataagata gacacccggc agcatgggga gagttttcat tttttctttt
    15181 aacataggat ctccttttac gctttgaata aaaagactat aacaaatttt gaaataatgg
    15241 acaagtttta aggaattcta tagttc
//
LOCUS       DAGCYL010000055        15237 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.056, whole genome shotgun sequence.
ACCESSION   DAGCYL010000055 DAGCYL010000000
VERSION     DAGCYL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.056"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            164..2761
                     /gene="adhE"
                     /locus_tag="MB216_001305"
                     /gene_synonym="adhC"
     CDS             164..2761
                     /gene="adhE"
                     /locus_tag="MB216_001305"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBT4526150.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2990..4096
                     /locus_tag="MB216_001306"
     CDS             2990..4096
                     /locus_tag="MB216_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBT4526151.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4113..5137
                     /locus_tag="MB216_001307"
                     /pseudo
     CDS             4113..5137
                     /locus_tag="MB216_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            5143..5622
                     /locus_tag="MB216_001308"
     CDS             5143..5622
                     /locus_tag="MB216_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBT4526152.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5638..6216
                     /locus_tag="MB216_001309"
     CDS             5638..6216
                     /locus_tag="MB216_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBT4526153.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGAFDVHPKS"
     gene            6248..7587
                     /locus_tag="MB216_001310"
     CDS             join(6248..6689,6689..7587)
                     /locus_tag="MB216_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBT4526154.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            complement(7627..8319)
                     /locus_tag="MB216_001311"
     CDS             complement(7627..8319)
                     /locus_tag="MB216_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBT4526155.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(8756..9325)
                     /locus_tag="MB216_001312"
     CDS             complement(8756..9325)
                     /locus_tag="MB216_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBT4526156.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(9399..9722)
                     /locus_tag="MB216_001313"
     CDS             complement(9399..9722)
                     /locus_tag="MB216_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526157.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(9876..10688)
                     /locus_tag="MB216_001314"
     CDS             complement(9876..10688)
                     /locus_tag="MB216_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526158.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            11042..11890
                     /locus_tag="MB216_001315"
     CDS             11042..11890
                     /locus_tag="MB216_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBT4526159.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            12035..12976
                     /locus_tag="MB216_001316"
     CDS             12035..12976
                     /locus_tag="MB216_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBT4526160.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            12966..15137
                     /locus_tag="MB216_001317"
                     /pseudo
     CDS             12966..15137
                     /locus_tag="MB216_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         4870 a         2785 c         2999 g         4583 t
ORIGIN      
        1 ctttatatat ttttctatac aaacacctgt aaataaagca attttatatt taaaaataac
       61 tgtgaaaaaa atcacaaaaa agtgtttaca tagaaaaata aatattgtat gatatacatg
      121 tgaattattt aacaaataac catgacccat taggaggata atgatggcta aagtaataga
      181 aaaagaaaaa acgaagacaa ttgacgtaca agcaatgatt gatgatttgg cagaaaaagc
      241 aaatgcagca ttgaaagaaa tggaaagctt tgatcaagaa aaagtagacc atatcgtgca
      301 tgaaatggca atggcagctt tggatcaaca tatgccatta gcgaagatgg ctgtagagga
      361 aacaggccgc ggtgtttacg aagataaagc aatcaaaaat atgtatgcgt ccgaatatat
      421 ttggcataat atcaaacatg ataaaacagt tggtgtgatc aatgaagatg tccaaaaagg
      481 gttgattgaa attgctgaac cagtcggtgt ggtttgcgga gtaactccaa caacgaatcc
      541 gacttccaca acgattttca aatcaatgat cgcgttgaaa acaagaaatc caatcgtttt
      601 tgctttccat ccaagtgccc aaaaatcttc tgcagaagca gcacgagtgg tacgtgatgc
      661 tgctatcgct gccggagctc ctgaaaattg tatccaatgg atcgaacatc cttcgatcga
      721 agcgacttct atgctaatga accatccagg tatcgcgatc gtcttagcga caggtggagc
      781 tggaatggtc aagtctgctt actctacagg aaaaccagct ttaggtgtag gtccaggtaa
      841 cgtaccagct tatattgaaa aaacagcaaa aattaaacgt gcagtgaacg atttgatcgt
      901 ttcgaaaaca tttgataatg gaatgatttg tgcttctgaa caagctgtga tcgtggataa
      961 agaaatctat gcagcagtaa aagcagaatt ccaagcacac caagtttata tcgtaaaacc
     1021 agatgaattg caaaaattag aagatgcagt gatgaacgaa ggaaaatatg cggtcaatcc
     1081 atcaatcgtt ggacattctg cgatggaaat cgcaaaattg gcaggaatca gtgtaccaaa
     1141 aggaacgaaa atgctgattg ctgaattgga aggcgtaggc ccagattatc cgctatcaag
     1201 agaaaaactt tctccagttc ttgccatgat caaagctaac aacacggacc atgcgtttga
     1261 tttgtgtgaa ggtatgttga atcttggagg attaggacat acagcagtta tccattcaga
     1321 aaacgaagag ttgcatgtca aattcggtct tcgaatgaaa gcttgtcgta ttttagtgaa
     1381 tacaccatct gcagaaggcg gcatcggtga tatctataac gaaatgattc cgtcattgac
     1441 tttgggatgc ggttcttatg gtaagaactc tgtttctcgt aacgtctctg ctgtaaactt
     1501 aatcaatgtc aaaactgtag cgaaacggag aaataacatg caatggttca aattaccgcc
     1561 aaaaatcttc tttgaaaaga actctctatt gtacttagag aaaatggaaa acgttgaacg
     1621 tgtcatgatc gtttgtgacc ctggtatggt ccaattcggt tactgcgata cagttcgtga
     1681 agtattggca cgacgtaaaa atgatgtgaa gatcgaagtc ttctcagatg ttgaaccgaa
     1741 cccatcgaca aataccgtat acgcaggaac gaaactgatg gcagacttca agccagatac
     1801 agtaatcgct cttggaggcg gttctgcaat ggacgcagct aagggaatgt ggatgttcta
     1861 tgaacaccca gacacatctt tctttggtgc aaaacaaaaa ttcttggata tccgtaaacg
     1921 gacatataag atcgacaaac cagaaaaaac acaattcgta tgtatcccga caacatcagg
     1981 gactggttct gaagtaacgc catttgcggt catcactgac agtgaaacac atgtgaaata
     2041 tccgttagca gattatgcat tgacgccaga tgtcgcaatc gtcgatcctc aattcgtcat
     2101 gtcagttcca gcatctgtca cagcagatac cgggatggat gtgttaacac atgcaatcga
     2161 atcctatgtt tcagtcatgg cttcagatta tacacgtgga ttgagcctac aagcaatcaa
     2221 gttagtcttt gactatttag aaaaatcagt aaaaacacct gatatggaat cacgtgaaaa
     2281 aatgcacaac gcttctacaa tggctggtat ggcgtttgct aacgcattcc taggtatttg
     2341 tcactcagtc gcacataaaa tcggcgggga atacgggatc cctcatggac ggacaaatgc
     2401 gattctttta ccgcatatta tccgctataa tgcaaaagat ccacaaaaac atgcgatgtt
     2461 ccctaaatat gactatttcc gtgcagatac agattacgca gatatcgcaa aattcttagg
     2521 tctgaaaggc gaaacaacag aggaattagt cgaagcttta gcaacggctg tttatgaatt
     2581 aggaaaatct gtcggcatca acatgagtct aaaagctcaa ggagtcacac aagaaacact
     2641 agatacaacc gtagaccgta tggctgaatt agcttacgaa gaccaatgta cgacagctaa
     2701 tccgaaagaa ccattgatct ctgaattgaa acagatcatc attgatgctt acaacggata
     2761 agcaaaagaa agcgtagaaa aaagataaga aaatagatga aaaagaactt ggaaaacgtt
     2821 gtttctccaa gttctttttt taatttccaa gtaactgaaa gttacttttg ttatttaaca
     2881 tttaggtcaa ttttagacag tttttgttca aaaagcgata tttatagtgg acggtattac
     2941 tgctatatta aggatataaa atgaaagcgt ttaaaaaggt ggcgtaaaga tgattattaa
     3001 agaagagatt tttgtaccta aagaattagt gaaagaaaaa attgacctgc tcgttgaaaa
     3061 cctgacaacg atcaaagacg ataatggtga atttttgttg gactttgatg gactgaaagt
     3121 agatgacaaa agctggacag tgtggaattg gccacaaggt gtcggtctat atggtattta
     3181 taaaaattac cagatgacaa aaaatcctcg cgcttatcaa gtggtcaatg aatggtttga
     3241 ggatcgtatg gaagaaggcg ctccgcccaa aaacgtcaat acaatggcac cgctattgac
     3301 tatggcttac ttgtatgaag acacgaaaga ttcacgctat ttgccttatt tagaacaatg
     3361 ggcagaatgg gtcatgaatg acatgcctcg gacaaatgaa gacgggttac agcatgccac
     3421 atatggaccg gaaaataaga atcagttgtg ggacgataca ttgatgatga cagtgcttcc
     3481 tttggcaaaa atcgggaaac tgttgaatcg cccagaatat ttagaagaag ctcgttacca
     3541 gtttatgatc catatcaaat acttgatgga taaacgaaca ggattgtggt atcacggctg
     3601 gacgtttgaa ggaaatcata actatgccga agcattttgg gctcgtggaa actgctggtt
     3661 gacgattgct attccagaga tcattgaaat cttagaatta tcgaaagaag atgcattgcg
     3721 caaattattg atcgaaacac ttgaagcaca agtgcgtgca ttaaaaactt atcagtctga
     3781 aagcggttta tggcatacgt tgttggatga tccttcttct tacgtagaat cttctgcaac
     3841 tgcaggattt gcttacggta ttttgaaagc ggtccataaa cgttatttac cacaagaata
     3901 taaagaagtc gcttatcgtg ctatccaagg attgcttgaa caaatcgatg aaaaaggaga
     3961 agttcaaaac gtttcaatcg ggacaggaat gggggacagt ttagattttt atcggaatat
     4021 cggaatcaca gcaatgcctt atggacagtc gttaacggtg ctttgtttga cagaattgct
     4081 cgtctcttat tgttaaaaga aaggaaaccc atatgaaaaa aacatcactg ctgtttgcag
     4141 caagttcgct tagcctaggt ctgctaggtc taagcggctg cggaaataat tctagtgtgg
     4201 caactgtcgc agatgcagac gtaaatgaga tagttacctt gcgctttgct tatgcaagca
     4261 acagccagta attgatgcaa tgaatgaatt tgggcgttta gtaaaagaaa aaacagatgg
     4321 ttccgtacag atccagtatt tcccagatgg acaactgggt ggagaacgtg aactgatcga
     4381 attgacacag tcaggagcag tagatttcac aaaagtaagt gcctcagcgt tggaaagttt
     4441 ctctaaagat tattcgattt tttccgttcc ttatctattc aatgatgaag aacatttctt
     4501 taaggtgatg gataatgaag aaataatgga gccaatctac caatcgacca aaaatttagg
     4561 atttaccgga ttgacctatt atgattctgg tcagcgcagc ttttatatgg tagacggtcc
     4621 gatccatacg ccggaagatt tgaagggaaa gaagatccgt gtgatgcaaa gcgaaaccgc
     4681 tatcaaaatg gtagagcttt taggcggttc gcccgtacct atgggaagtg atgaagtgta
     4741 cacgtctctt caatccaatt tgatcaacgg atcagaaaac aatgaatttg tcttgtatac
     4801 agctggacat ggcggggtag ctaaatacta ttcctatgat gagcatacac gtgtgccaga
     4861 tatcatcatc atgaatgatg ccatcaaaga acagctaact gatgaacagg aaaaagccat
     4921 tgaagaagca gcgaaagaat caacggtttt tgaaattgaa gcttttgcaa aagcaattga
     4981 agaagaaaaa gctttagcac aagaagaaca tggagtccaa ttcaatgatg tagataatac
     5041 accgtttcga gaggcagttg ccccacttca tgatatattc aaaaacgatc ctacctacaa
     5101 agacctttat caaaagatcc aagagcaagg agcgtagctg taatgaaaat gattttaaac
     5161 aaggtattag agatcctggg agctgttttg cttgttggga tggtcgctgt tgttttatgg
     5221 caagtatttt ccagaacggt tttaggaaat ccaaatacgg tcacggaaga gcttgttcga
     5281 ttcggtcttg tctggtttgc catgttgtct tccgcgtatg ttgtgggaca aaaagcacgt
     5341 ttagccgtaa cggttttaag tgaaaaatta actggcaaaa aaagtaaaat tttagaagtt
     5401 gttgttcaaa gtttatttct ggtatttgct gcgatgatca tggtgtatgg cggatggaat
     5461 gctgtcacat tgacaatggg gcagatctcg ccttctttaa gtattcctat gggatatgtt
     5521 tatctgtcag tacctgtatc gggggtactc atcattatct atagcctgat caatctaatc
     5581 gatacattaa gcaacaagcc agcaattcaa gaaaaaatat aaaaatggag gaataaaatg
     5641 gcaatagaag ccggattagt gttatttttt gtatttgttg tgttgcttgt attagggatg
     5701 ccgattgcta taagtattgc attctcatcg atgatgactt tattgttagt tataccgttt
     5761 gatgtttctg cttttagttc agctcaaaaa atggtgggaa gtatcaatag tttttcgctg
     5821 atcgctattc cgtttttcgt tctctcgggc attatcatga ataatggagg gatagctaag
     5881 aaactagtgg attttgcgat gttgttcgtc gggcgcgtgc cgggggcttt ggctcatacg
     5941 aatgtgttag ggaatgcttt atttggttcc atctctagct cggccattgc cgcttctacc
     6001 gcgattggtg gagtgttgat ccctcagcaa gtggaagaag gatatgaccg gaagtttgcg
     6061 acagcggtca atatcgcgtc agctccgaca ggtatggtca ttcctccaag tactgctttt
     6121 atcatgtatt cacttgttgc tggtggtgct tcgatctctg ctttgtttat gggaggttat
     6181 ctcgtaggcg cttttgatgt gcaccccaaa agttagacta gaaatctaat taaaaggggt
     6241 gctttttttg cggaaatata catttgtttt taagaaaaaa gtagtttcag actatttaaa
     6301 taacgaaggc ggctacaaat atcttgcaca taaatatcaa ataaatcgta cactggttag
     6361 acattgggta aggatttata actatcatgg ttgggaaggc ttggttggag gtggcaaaag
     6421 ctacactaca aaatttaaac ttgatgttat agaatatatg gaaacaaatg gtctttctat
     6481 ccaagaaact gctaaaaaat ttaatatcgg ttcaaataga actctaagta aatggataga
     6541 gcaatatgaa gaaggcggtg cttcttcact tgagagccaa aaaaggggca gaaaaattag
     6601 tatgaattcc aagctaaaca ttcctaaaaa acttaaagat gagtctcttg aagaagaagt
     6661 tattcgttta agagcagaga acgcatattt aaaaaagtta gaaaccttga ttcaagaaca
     6721 agatttgagc aagaagaaat caaggttaaa acaatccttc agctcaaaaa agaatttaaa
     6781 ttgaatctat tactttcaat tgcacaatta gcgaagtcaa cttattatta ctgggtaaaa
     6841 aaattagata agccagataa atatagcaag ataaagcaag aaattacagc gattgtaaaa
     6901 gaatcaagaa actcttatgg ttatcgtaga gtcactttag cgttaaagat gaaaggatat
     6961 acaatcaatc ataaaacagt tagaaaatta atgtcccaaa tgggacttac ctgtcaaatc
     7021 cgaataaagc gatataaatc ttataaagga acagtaggaa aaattgccaa gaatgtgtta
     7081 aaacgaaatt tttcagtaga tacacccaat aaaaaatggg tgacagacgt cactgaattc
     7141 aagataaaag gaagaaaaat ctatctatct cctatacttg atttatttaa cggagaaata
     7201 attagttata gtatatcaac tagtccaaca tataaactga ttgaagaaat gcttcaacaa
     7261 gcaattaaaa aaaagggaac tgagggttca ctgattctac attcagatca aggatggcag
     7321 tatcagatgc cacaatatca aaaaaaatta aaagagaata acattattca aagcatgtct
     7381 agaaaaggaa attgcttaga taattctgta atagaaaatt tttttggtgt gcttaaatca
     7441 gagttttttt atcgagaaaa attccgatca attgagatat ttcaaagtaa attaaatgag
     7501 tatattaggt ggtataataa caaaaggata aaactaaagt taaatggact atctccagta
     7561 gaatatcgga aacagtccat taaatagttc aacttttggg gttcagttca aagagggggg
     7621 gattttttat cgttgtggaa tatttaaata ccaacgcttg tcatgaaaat cttgtgctcc
     7681 acccttagta tttccttctg gatcgtttgt cgcacgcatc atgacgtata ctttcttatt
     7741 agggaaatca cgcatattga aagatacatg atagccaaca tttcctaaag tattataagc
     7801 ttgatttacg tctggtctat aaatcccatc agctcttact ctagctagtt cttttccagt
     7861 attgtagtcc ataatgaaaa tatactcgta tttataatta gcaatgtgcc atccagctac
     7921 atgcaagttt gcgttttcga tttctccgaa ctgatcaatg tgggcgtaat ttgttccatc
     7981 tgtcagcgta gaatttgcag cacctgctcg agttggatca attactggtt tatcctccga
     8041 agtagttgga ttatcatccg taaaaccatg agccaaatca taggctagtt tttctttact
     8101 tacacctatt tcagaaagat aaccgtaagg atctgtatga tcgccccaaa tattttgtgt
     8161 tacccataaa tgcgatttga ttcctggttg gttataagga gtgtctaatg ttaatggaat
     8221 accatatttc attgctgaat ctcttgccaa ttcaacgtat actttatagt tcttttcaaa
     8281 agtttcttta tcatgtgtgt gttgtaactc gatttgcaca ggacttttgg ctcacgaggt
     8341 tgtcctactc tacatcttgc tgatagatat gctgaaagga aaacaccaac attatgtttt
     8401 gctttttcag ctagaacgag acaagcagga atacctttag aagatatcaa aggtttttta
     8461 ggtcataaag atgtttccac tacacaagtc tatgcccata tttctcccga agtgaaaaaa
     8521 agatcaatga atcaactaga aaactatata gaagagcaaa taaaaaagca ctcaaattga
     8581 gtgctttttt aagctcacta tcactaaaac tatcactaat tttatttata agcgaacatg
     8641 tttctatcac tgatttttta aatctagcga aaaaccctta tcttaaaggc gttcgttcaa
     8701 ttctttggca aggtcttcaa agcctggctt gcctagaagt gcgaacatgg ttttcttata
     8761 gctcatgtag aaaaattttc ttgatgatga ttttatccat caattgagat tgactgtttt
     8821 gctctcctat gatttcagct atgattgtcc gattttgcgg ttttcttctt aactggtagt
     8881 tgattttccc agtttccatc aactcgctaa gcatatccac gactccgcca aatccggtct
     8941 ttttcgtaat aaaacctagt gctaccattc gttcatctag attttttgct gtaatcgtca
     9001 caccagtaat gagcgagtgc tgtaatgttt gggccagctc ttccatccct tcagcattgg
     9061 atacgacgga agctgttttg ttaataggtt cttcgtttat ttcttttggt tctttcctct
     9121 ccactttcac tgtttgacga aggacatcta gttcttgcaa gagcgtttcg tattgttctt
     9181 gggtcagcat cacgccagct actttttcac gattaaatat atatacgcca gttcccgcgt
     9241 ctcgcgcttg agcaaataca tccatcggcg atcgtttcac ttctgtgatc gaagatgtgg
     9301 gaacttctaa tttctttaat tccaatggat ttcctccttt actaatcgct ttttattata
     9361 gcacagactg acttgttttg atcttttttc caaaggtttt atcggttttt gcctaaccag
     9421 taattgatat cggtaagaag tctttcgata ccaaatgaag gatggtacag atacgttttt
     9481 tgatcttcgt tatggtctag tggaaaaaaa tgatcgatga tcactagatc tgcataccga
     9541 ggcggcttat ttcgagtaca tacatgtata gagatccgtt ttcgatgtct tagcgttttg
     9601 ttcagtaccc cagcaagtct tcttgcttct tggtgttcat agcgaaaaaa aaagacgatt
     9661 ttcacttcag gagggtggac atctaaatac cctcgttcct ggagtgtttt cagtaaaagc
     9721 atcccccact ctactgcggg gtgtgcaagt gcttgttgga tcgctagccg aaattgatat
     9781 agtttatctt tggaattggg aaatttttct aatgtttcct gataataacg aacttcttct
     9841 atcagccggt catggatacc aattacgtaa cgaagtcatt cttggtaaag tacttttgtc
     9901 agctttgatt tgctttcgct ctcaaattcg ctattcagcc aaaacagcga atcaagcttt
     9961 tcagtcacaa tcgtcaattt ccgatagtct tgtgtctctt ctatccgttt ccaccgcttt
    10021 tcagtaatct ggttacagct caagcgcaaa attgctagat aatttttttc ttttattggc
    10081 aaaatctgct cctctctaaa tagcaattca ggaaacaatt caaagaatag tgttcgactg
    10141 tctcctaaaa tctcgatttc tgccggatcc tttctttgct tcaataattg gatgaacatc
    10201 tcagaaaagg actgctctcc atcatctgca aaaaaatgcg gaaaatcttt caaaaagcaa
    10261 aacagcagat atcttacttt tctttctgaa ccaaagatct gtgctttttt ataagaaaaa
    10321 gaaaggtggt actcgccaaa ccactttctc aatcgctctt gcgtagaacg atcgtacttc
    10381 tgctcgtaag gttcattcaa tagcagattt ttcagtaaac aaaacggggg tgattggatc
    10441 agaaaaacaa acaagagata aggttcgcta tatccttgat cataaactaa ctgaacggtt
    10501 ttcttatgaa gaatcagctg aatgggacag tcgctttccg ccaaacgttt cagatgccga
    10561 tccactgttt taacatcgat tttcagttct tcagctaaat cagcttttcg ccagatcaaa
    10621 tctttcatta gattgattag tgaaaataaa ttctgtgcat tttttgataa tagtttttgc
    10681 catctcatcg atcatttctc acctgatttc taaacatatt gtttgatcaa aatgggttca
    10741 ctcctaaaaa gataaaaaac tccctttcta tttatttaaa tacgcaaatc ttcctcatta
    10801 ttttttattt tactcaattt aatcgtattc tcatttcaaa acgaatgtac tttaaaaaaa
    10861 cataataaga tcactttgct tgtaggatgt attcccttcc attacaatga aataaatagc
    10921 gattttatga gaaggtgatg tgaaaatgga agaaaaaata gaagaatcca aacaaaaaat
    10981 cgatcagttg atataagaat tagaaaaagc ggtcatcggc aaacgttcaa ctatccaatt
    11041 gatgatcgtg gccctattag caggtggtca tgtcttattc gaagatattc ccggtgtagg
    11101 taaaacgtta ctggtcaaag cactagcaaa agctgtacag ggaaccttca gcagagtcca
    11161 atgtactcct gatctgctgc ctagtgatat tcttggtttt tccgtataca attcacaatc
    11221 aaaagagttt gaatttcgtc cgggaccgat ctttaccacg atcttgttag cagatgagat
    11281 caatcgtacg actcctcgta ctcagtcagc acttttagaa gcaatggccg agaatcatgc
    11341 aacgatcgac aacaatacat accctctaga caatcatttt tttgtcttag ctacgcaaaa
    11401 tccaatcgag tacgaaggaa catatccttt gccggaagca cagctcgatc gttttttatt
    11461 ccgactacaa atcggttatc cttcttttga tgacgaactt ttgctacttt tggataaatt
    11521 cgaaaaagtg ttggataact taaatgaagt attgaacagt tatgaaatag aagaaataaa
    11581 aacaaatgtg gcgaatgtct ttgtcagtcc cgaagtggca tcttatgcct tgcagttagt
    11641 gtcagcaaca agaagtcatg ctgccatcca gcttggcatc agtccacgag gaagcctttc
    11701 ctttattcag gcagcaaaag cttatgcttt aatccatggg agaaattacg tcacgcccaa
    11761 agatttgcag gatttagttc cttacgtgtt ttcccatcgc ctgatttttt acgatcgttc
    11821 attgaaggaa gacgaaaaga aacaaatcat tcatactctt gttgcacaaa ttcctattcc
    11881 tgtgaggtag attgaggtag acaatggaaa aaatcaaaga ttatttcatt aattttgtgt
    11941 tatttctggt atatttcatc attgtgcttt ttgctcttgt attcaacaat tctttaggct
    12001 ggttcttgtt ggcctttttt actctttatg tatcatggag cttgatttca ttaagcagct
    12061 ttctaaagaa actttcggtt acttgtacat tgcagccact cgtttatcgc aaaaaaagta
    12121 tgcaggttat tttttccata gcaaaaaaga gaactttttc tttacctctc cctcgtttac
    12181 aaatcggctt cccagcaatc tttgcagaaa aaaacaagag attttgattc ttacaaataa
    12241 accgcaagaa gtccaattgc tttggcagcc aaaagaaaga ggatgccatg aaacattaac
    12301 tgtcattttt acagcctatg atagtctagg acttttcaga aaaagtgcaa aaagagagat
    12361 ctcttttcct gtgactgttc tgcctgcttt ttgtaagatt caagcagaaa aattacagag
    12421 agttctggaa aaaaaacagc agctgaacgc gtaccaaaga ggtcttgatt ttcgggaaca
    12481 tcgcgcttat cgtccaggtg attctttcaa ccgaatcgat tggaaattgt ctgctcatac
    12541 tcaagagtgg ttataccgtg aatacgatta tcaagaagaa gaatgttctc ctcttttttg
    12601 cttttggggt tcgcccagtc caaaatttga acctctgcta gacttgtatt tttctttatg
    12661 gcatttgaga aaagaaaagc agccggaact tctgatatta ggcaaccgcg cctttcatgg
    12721 caaagacccc ggtcatacct attttgcttc tttggaagct ggcgatgaga aaactttttt
    12781 cgcacacttg aaaaaatatg caaaaaaaca aatcgtcctc tttgttcctg agaattctcc
    12841 tgaagtctca gaagctattg aaacattgtc aaaagatcga acggtttatc ttgtttattt
    12901 cgtagaaaaa caactcatgg tccaagaaaa agacaagata tgttcattgc caggaggtga
    12961 atggattgac gattaaacgg gaaaaatggc tgcgtggcgt ttactccttt gtgatactaa
    13021 gtgtaggtgc tccgatattt gtagtagcta atcaattgca aaacagctac cttcttatcc
    13081 taactattgg tgtcctatgc cttattgtct gctgtattcc taaaaaatgg gtacgtttgc
    13141 tgctttcttt gccagtaatg ataggttgtt tgtttttata ttttccttcg aagacatttt
    13201 ccatattatg gatcttacag tttttttcgt cagttacaga ggaattcttc catctatccc
    13261 aaggtaaact ttattatttg ccagaaaaga cagctttttt tctcataatg ttgctgatct
    13321 atttatttat tacattgacc gtggattacg actattggta cgcacccttt ctttctttga
    13381 tggcttactt catgatgctt accgtttttc ggaaaaaaga tttactgtac gaaatgattg
    13441 cggtagtaac tgttttgttc gtgtatctgg cagcaaggca atttttctcc atccggttgt
    13501 tgtcgagaaa cactcgattc cagtcattga ctacagttct gcttatttgt ttcttaactt
    13561 ttagcgctat cttgccttta tatcttccga aagtacacca atctctcgag gaaacgagtg
    13621 aaccgatccg agaatatctt aatgatgaag ggctttatga cacactccat gagtatcaat
    13681 taaccggaag tgcaagaaca ggcttcagtg agaatgatga acagcttggt ggacctttat
    13741 atgataatgg cgaagttgtt tttacagcga accaaaaagg aaatggctat tggaagatcg
    13801 aaaataaaaa tcattacacc ggttctggat gaatagaaga tcaggacgca cccatccaac
    13861 caatccaaga attccctgtt gacatcacga gctaccaaac cgttatagaa gacaaagaaa
    13921 acattacatt gaccctaaaa aaacaagctt tcttacctta tccttatgga aatactacct
    13981 tgactgattt ttcattagct agtggacaaa cgctttacta tttgcctgaa agccagcgat
    14041 tcattcctgt cccgaatgaa tcgacacgtt ctgcaacttt acaaacagaa aggttagttt
    14101 ttacgagaga agaattaaaa agcagtcagt taaaagctga tgatttagaa aatcagcaga
    14161 caaatcttca attgccaaaa agtttgccta aacgtattga ggacttggcg gtcgaattga
    14221 ccaaaaatga agcaacttta attcgtaaag tcgaagctgt ctaacagtat ttaaaaacaa
    14281 gcggaaattt ccgctattcc aaaacggata ccgggcatac tccaaaagaa cacgattatg
    14341 tggattattt cctctttgat tcaaaagtcg gctattgtga caacttttca acttctatgg
    14401 tcgtccttct tcgaacactt ggtatcccag ctcgctggac aaaaggtttt acaaatgggc
    14461 tcagaacagg cacagataat caagggaatg aggaatacag cattctcaat agtcatgctc
    14521 attcttggcc agaggtatac tttcctggat ttggctggct tccatttgaa ccaacccctt
    14581 catttgccaa tccaatgggt tcacaaccag ttgctgaaac aaccgaaacc gtccagcctg
    14641 ctatcgaaca accaaatgaa agcgaaaata tggcagaaac tatttccagt gagagatctg
    14701 aggtcattcc atcagaagaa acaacactca caccagctta taaaatcgtc ttattcagcc
    14761 ttggagtgtg tttatccgtc ttgttgttct ttctattgtt attcagaaaa tctctgcgct
    14821 ggacaagtta tcttatctat cttcattttt ttgcttctac taatacgatt gcatatcaaa
    14881 aacttcttaa ccctttggaa acattggtac cgcgcaaacc cgcagaatct ttgcaggaat
    14941 atagcctgcg aatagaacaa gaattcaagc tagatctgcg tttcattcga ttaactactc
    15001 aatatgaagc agaaatatat ggacgacaag cagatccggt aaaattcgat aaaaaaacct
    15061 atttagaaat aacgcatata ataagaaaaa cggtatacaa aaaaattctg acaaaaggga
    15121 gccgcttcaa aaaataagat aaaatttctg gagaatctac ttctcgaatg ccgagaacca
    15181 gctgattgag tatcgattac tcgaaaaaaa ggttgtgaca cgttttttgt cacaacc
//
LOCUS       DAGCYL010000056        15187 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.057, whole genome shotgun sequence.
ACCESSION   DAGCYL010000056 DAGCYL010000000
VERSION     DAGCYL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.057"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="MB216_001318"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="MB216_001318"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBT4526161.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="MB216_001319"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="MB216_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBT4526162.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="MB216_001320"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="MB216_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBT4526163.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="MB216_001321"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="MB216_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBT4526164.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="MB216_001322"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="MB216_001322"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBT4526165.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="MB216_001323"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="MB216_001323"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBT4526166.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="MB216_001324"
     CDS             complement(8053..8523)
                     /locus_tag="MB216_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBT4526167.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="MB216_001325"
     CDS             complement(8569..8994)
                     /locus_tag="MB216_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBT4526168.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="MB216_001326"
     CDS             complement(9061..9330)
                     /locus_tag="MB216_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBT4526169.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="MB216_001327"
     CDS             complement(9341..10936)
                     /locus_tag="MB216_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBT4526170.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="MB216_001328"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="MB216_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBT4526171.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="MB216_001329"
     CDS             complement(14489..15049)
                     /locus_tag="MB216_001329"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBT4526172.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
BASE COUNT         3926 a         3436 c         2695 g         5130 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagag
//
LOCUS       DAGCYL010000057        15144 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.058, whole genome shotgun sequence.
ACCESSION   DAGCYL010000057 DAGCYL010000000
VERSION     DAGCYL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.058"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            10..156
                     /locus_tag="MB216_001330"
     CDS             10..156
                     /locus_tag="MB216_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBT4526173.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
     gene            complement(218..2029)
                     /locus_tag="MB216_001331"
     CDS             complement(218..2029)
                     /locus_tag="MB216_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBT4526174.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2026..2391)
                     /locus_tag="MB216_001332"
     CDS             complement(2026..2391)
                     /locus_tag="MB216_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBT4526175.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2554..2949)
                     /locus_tag="MB216_001333"
     CDS             complement(2554..2949)
                     /locus_tag="MB216_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBT4526176.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2967..3929)
                     /locus_tag="MB216_001334"
     CDS             complement(2967..3929)
                     /locus_tag="MB216_001334"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBT4526177.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3929..4141)
                     /locus_tag="MB216_001335"
     CDS             complement(3929..4141)
                     /locus_tag="MB216_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBT4526178.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4162..4860)
                     /locus_tag="MB216_001336"
     CDS             complement(4162..4860)
                     /locus_tag="MB216_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBT4526179.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4880..5422)
                     /locus_tag="MB216_001337"
     CDS             complement(4880..5422)
                     /locus_tag="MB216_001337"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBT4526180.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5554..6558)
                     /locus_tag="MB216_001338"
     CDS             complement(5554..6558)
                     /locus_tag="MB216_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBT4526181.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6555..7544)
                     /locus_tag="MB216_001339"
     CDS             complement(6555..7544)
                     /locus_tag="MB216_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBT4526182.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7541..8347)
                     /locus_tag="MB216_001340"
     CDS             complement(7541..8347)
                     /locus_tag="MB216_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4526183.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8513..9469
                     /locus_tag="MB216_001341"
     CDS             8513..9469
                     /locus_tag="MB216_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4526184.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9546..10064)
                     /locus_tag="MB216_001342"
     CDS             complement(9546..10064)
                     /locus_tag="MB216_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBT4526185.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10152..10301)
                     /gene="rpmG"
                     /locus_tag="MB216_001343"
     CDS             complement(10152..10301)
                     /gene="rpmG"
                     /locus_tag="MB216_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBT4526186.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10529..10975
                     /locus_tag="MB216_001344"
     CDS             10529..10975
                     /locus_tag="MB216_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBT4526187.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11170..13065)
                     /gene="asnB"
                     /locus_tag="MB216_001345"
     CDS             complement(11170..13065)
                     /gene="asnB"
                     /locus_tag="MB216_001345"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBT4526188.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(13390..14364)
                     /gene="bsh"
                     /locus_tag="MB216_001346"
     CDS             complement(13390..14364)
                     /gene="bsh"
                     /locus_tag="MB216_001346"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBT4526189.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(14910..14983)
                     /locus_tag="MB216_001347"
     tRNA            complement(14910..14983)
                     /locus_tag="MB216_001347"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14947..14949),aa:Ile,seq:cat)
BASE COUNT         4715 a         3167 c         2580 g         4682 t
ORIGIN      
        1 gtatctggaa tattagtagg aagtctggca acagccgctg ccgttgctgg tttggttgct
       61 tctgtgaaaa aaacagtgat cgatccaatt gacgaaaaag aagcaatgat cgaagaaaac
      121 cgtaaaaaag caatgcgtaa acgcgtttct cgttaataat tatttaaaaa tacgcatgaa
      181 gactttctaa aaaacagata agtcatcatg cgtattttta tttgtcagtc aatataagtt
      241 gtgctatttt atctgcctgt ctcagattcc atggatctgt ataaggtcta gctactgctg
      301 cagttagcca atccacacct tcaacaggta ttccatagca ataaaagtca gactggattt
      361 cccctgaagc atcaatcacg cgattcgtac ctcgctcaac atctatagct tctgaatgat
      421 aacttcctgt attatctgaa aatgaaaaag ggcgaacaag tttgttttcc ttcaaatttt
      481 ttatgacagg attacttgaa cgagaaaagc ttgtttgtgg caatcgagct tcaatcaaat
      541 agcgactgct gatttcttta tcttgtagag aaataacata gcggttgtgc ttttgctgaa
      601 tatcaaatgg cggttcgatc aaatgaaaaa tacccgcatc tatcagtgct gctaattctt
      661 tcgtccgaat gattggcgga ccgatcgtta aaaagctatt tagatgagta aaccatcccc
      721 atagtatttc tttgtattcc ttagcagaaa aaacgtgcca ttcaatagcc tgccggattg
      781 gatcacgcca gtcttttaat gcatcaaatg ttgaagcaaa tgctcctgtg caattgcctt
      841 tttccgcttc ttttatctgc ccttcaataa atattcggct agcttctgaa aagggctcat
      901 tctgataggc aagaggagtt tctaaatacg accaatccca tctataaggg attagtgctg
      961 gatgctttcc gagacactcc tctgaagaga gggagagaaa atcatgctca aaatttttct
     1021 ttggaatcgg caagtgatat tcttcgatga ttttcttgta ataaaacaac tcaacgtctt
     1081 ttttcagata gtcaaaaaat gtttcgccag tcaaaaatcc cgactgatgc cattgctcca
     1141 ggttttcttt tgttagtaac cgtggccatg ccattgctcc gccttgcttt tgatttcttc
     1201 ctcgagggta ataagggagc ccccttcttg atccactgta tacaatcggt tccttgcctg
     1261 atggatgata gaccagtttt cccttttcgt tctcgaagcg ccctcctctt gatactgtaa
     1321 acaatccgac ataatcaaaa aaacttaagc ctagacctct taaaaagaca ttttctttag
     1381 gcggtatctt cgaaagatct acatctgcag gattagaagg aagctgatac agtaaaacat
     1441 ttttctttgc gtagtctacc cacactcttt cttcttctgt ctcatcattt tcccagtgac
     1501 cgcttgctaa aacaagctga tcgatagaat agctttgctg atctgtctgt aaaagataac
     1561 catcttttct tttgcttact ttttttacta aagaataaac aaactgaata gaagctggaa
     1621 attcttcttg caactttgag aaaaaccagc gttgatagag tccgtataaa catcgagtag
     1681 attgctgatc tttttccaga cgattgattt cttctataat tagttgctta tttttggtta
     1741 ccgtctgttc gatataatct gtagcaggtc ctttactcca ttgaaacaag ttgggtccct
     1801 gtgttaccaa tcctccactg gacaatgttt catctgtaaa aagcgtcact tgttgagaaa
     1861 cagaattcat taataactcc ggtggctgat tcaaccgcca aactcgacct ccaggaccat
     1921 tgggatcgaa aagaaaaagt tcaagagaat gtctgtcctg attattatgg agaatccttt
     1981 ctgctacact taatcctcta ggtccggcac caatgatccc tattttcatt tgcatgcctc
     2041 catttcgata aattttttca ataaaacagc atggttctga tgttcatttt ttgcaccata
     2101 aacaaaagta atgactggat gttctttcat aagttgactg agtttaaaaa tagcttgctt
     2161 cttttcttct tcgaaaatca attcttgctg gtatttcttt gcaaatgaag caaatcgttc
     2221 tggtacatgg tcaaaagttt tgcgtgcttc attactaggt gcaatttctt tcaaccacag
     2281 atctaactgc gctttttctt ttgaaattcc tcgtggccag aatctatcca cgagtattct
     2341 atagccatcc tctttttcag catcaaggta aacacgtttt aatttgatca tagttgtttc
     2401 cttctttcta ccttttcgct tagcttatat atgtagataa tagcacaaaa taaaaaaaat
     2461 gaacggcaca actatccatt gcgccgttca tgtgatcttg gtcaaaatct aatgattgat
     2521 tcggttttta aagctgtcat acaatcatct gttttatact tttttacttt tgatttttcc
     2581 agaccatccg tcatatccac ccttaagtaa ataaatctct tgataacctg ctttacgtaa
     2641 tttatttgct gtacggatgc tcaaactttt cttttgatcg taaatataga caggctggtc
     2701 ttttcgaata gatcctaatg aatctttcaa aacagtgtaa gggatgtttc ttgcacctaa
     2761 aatatgccca gcatcaaaca catctttttc gcgaacatcg attacttgtg cttttctcat
     2821 tccttctcga aattcttctt ctgtcaaagt cgttgctgaa cgtttggcca ttactcgtag
     2881 atagacttcc caaaggatga ttgcaagcac aatcaatagc aaaatgatat taattaccca
     2941 taaaaccatg taatgcctcc gtttttttaa gcaaatttca aggctaatga ggcagcacca
     3001 atgacaccag cttcattacc aagctctgct aatttgattt ttgttgattc tcttacttgc
     3061 gggaaagtaa attctttaaa atatttctct acacggctac gtaaaaattc tcctgctgcc
     3121 gatactcctc cacctaacac gatacttgac ggatttaatg tattccccaa gtttccgcaa
     3181 gctaaaccta agtagaagca tacgcgatca acaaccatca atgcaaacgg atcgtcttct
     3241 tgagccattt caaagatatc tttacttgtt acttcttctc cgttgtctag cattgctttc
     3301 agttttgaat cccctgcgta ttcttctgcc aagaaacgac ctaggcgtac cacacctgta
     3361 gcgctagata cagtttctaa acatccgcgt ttaccgcaag tacattcgaa cccttcagga
     3421 tccacagtga tatgtccgat ttctcctcca gctccgctca cgccgtgaat caaatttccg
     3481 ccagcaacga tacctccgcc aacacctgtt cctagagtaa cgaatacaac gtctggatcg
     3541 ttttctccag cacctttcca tcgttcacct aaagcagcaa cattcgcgtc attatcgata
     3601 gcgaatttga ttcctgttcc tgcctcgatc agttttttca caaattgaac ttctgtccag
     3661 tttaagttat acgcaccaat aaccgttcca gcttcgctgt ccacgcttcc tggggtaccc
     3721 atgccgattc cgataaagtc ttcggctttc atattatata aattcaaacg atgattgatt
     3781 gattcaatga tttcaggaac gatatgctta ccatcatcca aaatattggt atcgatgctc
     3841 catttttgct gaatctctcc ttctggtgtt aaaattgcaa attttgctgt tgttccacct
     3901 aaatcaatcc caataatctt cttgtccatt acaaatttcc tttctttcgg ctttcttcaa
     3961 ttcggtgctc tctttttaat atgcgccacg ctgtcatgta tgtatcatga tcaatcaaac
     4021 ggctatcata cagattacgc aattcgatag tcattaattc aatgtcatag atgcgtgcac
     4081 ccacatagat aaagatgcca aattttttga acagctgctg tacatcatat aagctctcca
     4141 tctttaagca cttccttcat attatacaag taaaccatat ttttttaaac ccagtaaaat
     4201 acatattaca aaaagaaata caaaaactag tgtagaaaga atcctttggt gtatcgaata
     4261 gcttgtctgt tgtttcggta gacccaaaac attccctaac actaatccgc caattagccc
     4321 accgacatgt ccccaaacat ctacagaggt atccagaaga ccaaaaacaa atgtcaacac
     4381 aataaataaa agaaaatgtc tgactagctg ctggatcgct gggttatgtt taaaatgaaa
     4441 ccctaagata aacaacgcac cgaacaaacc gaatatcgaa gtacttgcac caccagacag
     4501 aacattcgct tcgttaaaag caaagcttgc tgtatttccc attatcccgc tgaacatata
     4561 aatcaagaaa aaacgccaat ggccatagat tgcctcgatt tgctgcccca taaaatacag
     4621 tatcagtgag ttcattacga aatgcatcag tccaaaatgg atgaagattg gtgtcactaa
     4681 acgccaccat tcattgaagt gaacaagata aggaccatac attccggcat aataagggat
     4741 ctcaagtcgc ggaaataatt ccatcatgag gaagaccgct gtctgtatcc ctaaaaaagc
     4801 ataggtccat aacggtcgtc ttaaccatgc acgcaatttt atttgctgtt gataattcat
     4861 gaaaacctcc catttcattt tagtcaataa acaattgttg gatcttctga tcaaagctct
     4921 ccggttgcca ttgttcattc aattgttcac gaaaaacaag gctacatgtt tttttatgaa
     4981 aattatttaa ataccggtcg taataaccac cgccaaaacc gattcgataa ccggctctat
     5041 tgtagataat acctggtaca atgaggagat cgatcatctc cggcaaatag agcttctctg
     5101 attgtggctc aagaacacca aaactcgaac gtacaagaac ggactgttca tccatttcat
     5161 aaaacgccat ctgacgtttc ggcaaagttt taggtacaac tattttcttt tgttcttttt
     5221 ctgctcgttg gataaccggc tgtgtatcca gttcaaattc attcgataaa gtcgtcccta
     5281 tcacttttgc tgacttccag tactcgctgg cgaatagctg tttatagatt cgctgttctt
     5341 tttccttttt cttgccactt ttggccagat ttttcagctt ttcgattcct ttccggcgta
     5401 atcttacttt caattggtcc aaaaactcac ccttccttct ctcataaaat ggtataggaa
     5461 aaaaagaaaa atagcaatcg aactttcaaa taaatgcata aatagaataa aaagaagacc
     5521 tagaaatgat tggccttcct tttttaatga atttcaactg ttcagcgcgt gtttttgtct
     5581 gataaaaagt gcatacagaa aatatggcgc accaaaaagg gcaatcaaaa ttcctgtagg
     5641 tacttcccca cttagtagaa tcactctagc aaccatatct gcactaagca gaaaaatgga
     5701 accgaacaaa ccgctcagaa caaaggtcca gttattttct ttacgtacga ctagcttcgc
     5761 agcatgagga gctagtagtc ctacaaaaga taaactacct gtcaccgcta cactaccgct
     5821 tgccaaagca acagctaaca acaaaaggat tttttgactt ttagccacgt taaggcctaa
     5881 tccttgcgcg cgttcttgac caaatgatag caatacaatc ctctgtgcat aaagtcctcc
     5941 tagcggaatt aagataaccg cccatggaag caatgcccaa acataaggcc agctcgcacc
     6001 ccagattgat cccgctaacc atgcattcac aaatccataa ttttccttag ataattggat
     6061 cgtgcatagc aatgttaatg cagaaaggcc ggcattaacg gcaataccgg acaataagat
     6121 tcgattcata cccaaaaagc catttcgatc ataggccgca agatagacac agcaagcagc
     6181 taaaacacca cctagaaagg ctacaactag caatagaaac ggcagcgaat agtgcgaaaa
     6241 gaaacctaaa taaagcatca ctaaaaatcc tgctccggca ttaatgccca atatactaga
     6301 atctgccaat tcatttcttg ttactccttg caagatataa ccagaaacgc caagacaagc
     6361 tcctgctaat aaactgatca gtaatcgagg aactcgaaat tctctaacaa tcagcgccaa
     6421 agaagatgat tctttcccta tgaacgtggc aaaaagctga tccaggtaaa taaaaggcgt
     6481 accaatcatt aaactaccta tagacaggat gacaagtaca aaaagcaaga aatagaacgt
     6541 ccaatatatt tttttcaccg ttgttccctc cttacttgta tcagtaaaaa cggaacgcca
     6601 atcaatgcaa tgactaatcc aaaaggtgtt tcaaaagggg gattgatcat cctcgctgta
     6661 aaatccgcca acataaccaa tccaccgcca actaaaaaag agacaggtag aacataacga
     6721 tagtccgttc caaccattcc gcgaacaact ggcggaacca acagcccaat aaatgaaaca
     6781 ggtccgacta acgaaacggc aatggctgct aacactgcaa ctagacagaa aacaatcaac
     6841 cgcgttcgtt taggatgact tccaagagct aatgcatgtg tttcactcaa atgaatcaaa
     6901 gatattgctt ttcctaatga aaaagaaacc aaaacagcaa gtatatataa aggaaacaat
     6961 actgctaatt gtttccatgt gatattggca gctccgccta caaaccaaaa agcaagatcc
     7021 tgctgcagat taaagaattg ggtaagtgct tggctgatag cagtaaaaaa agaactaata
     7081 gctacgccag ctaaaatcag ctgtaaagga ttcatgccca ttttcgaaaa ctgcaaaaaa
     7141 ccaaatacga gcaaagttac agcaaaagaa cctgccaatg acattagaaa cacagaaaaa
     7201 tcagaagctt taggccaaaa agcaaaacta agggccattg ccaacgaagc acccgcgttg
     7261 attcctagta agcctgaatc tgctaaagga tttcttgtca ctccttgcat caatgcaccg
     7321 ctcaatgcaa aacaactccc tacaagaaat gctcccgcca cacgaggcaa acgaatattt
     7381 cgtatgattt ggtgactttg ttttgatcta tcaaaatcaa acaacgcctg ccaaaaatct
     7441 gcttttccca cagtcgctgc gccatttgaa agagcaacaa aaaaaataat caaaagaaca
     7501 aaacagacca aaagcaggag tctatttttc ttcatagtcc tcataattga tcctcctgct
     7561 cttttggcaa caaatcatag gttagaaaaa ctggtttatt tgttcttgga agcgtgacaa
     7621 tcgttgcatc aatctcaaat atttttttca tacactcttt tgtgatcact tcttcaggtg
     7681 taccttctgt gatcaactta ccgtttttca ttgctaataa ataatctgaa aaacgtgaag
     7741 catgattaat gtcatgaatc gacatcaaga tcgttgtttg tttcatttga ttgatttcag
     7801 tcaataactg taggatctct aattggtgtg ccggatctaa aaatgttgtc ggttcatcca
     7861 agatcaagat atctgtttcc tgagcaagcg ccatagcgat ccagacacgc tgggtttgtc
     7921 caccagacag cgttgcgata tcccgcatac gaaatccagc tagacctgta gcatccaacg
     7981 cccactcaat tgcttcatga tcttgcgtgt ccatgccact gaacagtttt tgataagggt
     8041 atcgtccgta agatactact tcttctactg tcatacctgt agggtgttca ctagtttgtg
     8101 ctaataatgc aatttttttt gcaagttctt ttgaactgta tgtagagata tcagagttat
     8161 tgacaatgat ctttcctgat ttcacaggta gtatacgggt aaaacttttc aataaagtag
     8221 attttccact tccatttggc ccaatcaaac tagtgatttt cccttcagga atcgtaacag
     8281 acatgttttc aatgattttt ttgttttcgt agccggctgc aatgtcagct gctcttaatc
     8341 tcgtcatttc acccatcctt tcgctgaaaa tcattctcaa ttatgacgct gtttcaacat
     8401 ttcgtcaagc gctactttca tatctatagc caaatgaagc ggttgacagc gttcgaaata
     8461 tcaggaataa taaccaatga gaaagattct caattatgtt aggaggaaag aaatgaaatt
     8521 aaaaacaaaa gtattcacat tatctagcat attgctgctg gctggagtac tgagcgcttg
     8581 ctccccctct tcagataaag aaacagctac aactgccaat tcgacgactt catccaatag
     8641 tactcataca gtcacagata cacttggaca taaagtagag attccaaacg cgcccaaacg
     8701 gatcatcggc agttacttag aagactatct gattgcacta ggggaaaaac ctatcgccca
     8761 gtggactgtt ggcagtggtt ccattcaaga ttatctgcaa gatgatttaa aggatgtccc
     8821 aacgatttct tatgaccttc cttatgagaa agtgttaagc tttgagcctg atttgctgtt
     8881 gatttcttct tccgctactg tcgaaggcgg gaaatacgaa caatatagca aaatcgcgcc
     8941 aacttatgtt gtaaaaaacg gaacagatgt cacatgggaa gaacaactaa aagatgtcgg
     9001 caaagctttg gcaaaagaaa aagaggcaga aaaaatcatt tccgattatc aaaaatccat
     9061 caagaaaact cgagaagaac tagcagataa aatcgagaac aagacagctg cagtgttgtg
     9121 ggtgaccaac aactcagcct ttatggtcag caaaaatcgg tcaagtggaa gaatcgtata
     9181 cgacgatctg gaatttggtg ttcctgatct agtggaagag gtctccaaaa aagccacttc
     9241 tgactggtct gctgtttcct ctgaaaagct agctgaatta gatgctgact acatcatcct
     9301 tgtgaacagt gacaaagatg ctgccatgtt caatgaacca agctggaaga atctaaaagc
     9361 agtgacagac aatcatgtac tgcaattcgg acctgaatct agttggctat ataacggacc
     9421 tattgccagt caggaaatgg tcaatgatat caaagaaaaa ctaaattgat tttaactaaa
     9481 aaaagcatct tcacgattag tcgtgagatg ctttttttct tattaacaaa ctagacgcat
     9541 agattttatt cttcttttga agcaacccta ttttttactc tttcccattt ttgaaaacga
     9601 tgggcatata gattttcttg atccgttatc ccctcatact cctctataag ggcgaagtct
     9661 gaaaaatcaa tattcccaat atacgtatcg ccttcaaacg tgctatcgat caatgttcgc
     9721 cagatccgac cagtgtaagg aagaagttct tgaaaaatcc gactaccacc cgaaacatag
     9781 atatcttctg agatcgtttg actatacgac aaaagttcat cgatcgaatg cattacttcc
     9841 gcattttctt tcgtgcgaaa atttggttgc gttgtcaaca cgatgatatg gcgataagga
     9901 agtgaaagat cccccattcc ttcataggtc tttcttccca tcactaaaat tcgatccatt
     9961 gtgtgttcgc gaaagaaacg catatcattt ggcaatcgcc atggtaacaa accatttttg
    10021 ccgatcagac catttttatc ttgtgcccac atggaaataa acatattatc cgctccttat
    10081 gattttagat gttttgagat aaaaaaagtc tcaattcttt gaccagcaag gaattgagaa
    10141 ctttaaaaac attattttgt ttcacggtgc aaagtaactt tgcgttcgcg tgggcaatat
    10201 ttttgttttt ccaaacgatc aggattgtta cgtttgtttt tgcttgttag atagttacgt
    10261 tctttacaag aagtacactc taaagtgatg tttacgcgca tgtttttccc tcccattttt
    10321 acaagaatcc tctaagatga ctctcagact tgattatctt accatgattt tttctagatt
    10381 gctagtgttt ttcacgattt tgttaagtaa aactacttga cgataaatac aacggtttta
    10441 cttctcaaac aaaatctttt tctttttgta taaataccct tacacttgtt tatatagatt
    10501 atgtttttta ataaaggggg atactaatat gccatggaac atgaaggact ttcctgcatc
    10561 aatgaaaaac ttagataaat tgacaagaaa aaaagcaatc gacatcgcaa atgcattatt
    10621 agatgaaggg taccctgata accgtgcgat cccaattgcc attgatcaag caaaaaaatg
    10681 gtatgaagat gctagtgaat cagaaaaacg aactttcgaa aaagaaaaaa atccaagcaa
    10741 gacggatgaa cacgatacga atcctcgtgc tggaaaactg cttgattcag atgtgatcgt
    10801 agaatatgag gaagaacaat ggatcgtgaa atctaaaggg gcaaaaaagg caagcaacca
    10861 ttttgataca aaaaaagaag caatcgaaaa aggaaagcaa gtagcacaaa acaaagaatc
    10921 aactttgttc atttataaaa aagatgggac aaaagaaaaa gaaatcgatt attgaacata
    10981 tgtaaagggg ttgtgacgaa aatcgtgtca caaccccctt atgttcgaaa caatggtgtt
    11041 cctctgccaa ctccgaacca taagtcataa tatctataaa acgggaagag ctgttttttg
    11101 aaattgtttc ttatcactca gtgttaaacg gtaggagaaa acctcttttt tattgatatt
    11161 tgattttcat caaacagtca catagtcgat tgcttctgct tgcggaatcg tttcatctac
    11221 aaagtagact ttataccatg tcaagaaact gaagattgtc cagatttttc tttgctcttc
    11281 tttacgtcct tcgtgatggt catctagcaa cttcatgatt ttttcttgat caaaaaattc
    11341 ttttgcaaaa tctgcttcga ataatgcttt gacttgttcg tagccgtgtt cttctttcaa
    11401 ccaagctttg attggcacag ggaatcctaa tttcacacga ttcgaccatt cttccggaag
    11461 atgtttgttc gcagcttgtc tgaaaatatc ttttgtgttg ttttgattca ataaaaactt
    11521 acttggtatt tgttgagcca atttcatgac ttcgatatcc aataaaggta ctcggacttc
    11581 caaagaactt gccatagaaa gtttatctgc ctttaacaag atatcttttg gcatccattg
    11641 atgaagatcg acatattgca tcttattgac ttcatctttg attccttctg ttttctcgta
    11701 atgaacagtc atgatttcat ccacagatgg tgctgtttga tattttggtg ttaacacttc
    11761 tgatgcttct gattcttcga acactcgagc atgaccgata aaatattctt ttgcaggagc
    11821 aagtgattca tataaatgga tccgtccatg gaaatttttc atttttccga ttttacgtcc
    11881 taaattatag cgtgttcctt ttggtaattt tttcaatcct tgagcaatca cacggatgaa
    11941 ttttgaattc gtatggaatc catacgtttg gtatcccgca aacagttcat ccgccccttc
    12001 tccagattgg actacccgca cactttgtga agcaagattt gctaaaaagt acaaaggtac
    12061 gcaagaagga tttgaatcag gttcatctaa gtgatactgg atcaaaggga atgctttgaa
    12121 cgacatgtct ccgtcgatca ctgctgctgt attatctaag tccaatagtt cggttagttt
    12181 tctggcttca atcgcttcat tataggtttt atcatcaaac ccgattgaga aagaatgatc
    12241 tggtttcaat acagaggtta cataactgga atcaaccccg ctggataaga aagaaccaac
    12301 ttctacatca ctgaccgtat gtgcttcaat agaagcttga acggtttcgt cgattttttc
    12361 gatccattct tgtcgggaat gcgtttcttt tgtttcaaaa tctgcatccc aatattgctg
    12421 aatgtctatt tttccatctt tatacgtata ataatggcct tcaggcaaac gatagacatc
    12481 tttgaagaat gtttcgccgt ttaatggaga atattggaaa gtcatataag gtttcaacgc
    12541 ttctttgttc aattctttgt taaaatcagg atgaggcaga aaacttttga tttctgaacc
    12601 aaacataaat gttccgttca tttctgcata ataatatggt ttgatcccaa agtggtctct
    12661 tgcaccgaat aactcattct tttcattatc ccaaatagca aaagcaaaca ttccgcggat
    12721 cttttgcaat aattctgttc cccattcttc gtaaccatgc aaaaggactt ctgtatctgc
    12781 atgtgtttga aaaacgtggc cagctgcaat caattcttct ctcaaaggct ggtaattata
    12841 gatttcgcca ttaaaaataa tgatctttgt ccgatcttca ttgtaaatag gctgagtacc
    12901 gccctctaag tcaataatac ttaatcttct gaatcctaaa gcaacatgtt tatcgatata
    12961 ttctcctgaa ctattaggtc cacggtgaac gattcgatcc atcattgtat taataattgt
    13021 ttttttgttg tccttgtcgt taacaaaacc tacaataccg cacatttaac tttcacatcc
    13081 taatataaaa ttactgttta tttcattcat atgcatctta tcatagaata aatagcggac
    13141 gaatattttc tctcttacaa tttcgtcacg tgaaaacaaa agatgaaagt ttctgttttt
    13201 acagaaaata aagtcccaat atctaattta ccatgttttc ttttttgagg ctaccttatt
    13261 ctcttcgtgt taaaaaaata ttagtggatg ctatttccaa aaaaaccagc aaaaaaagac
    13321 acgacctaaa gatcgtgtct aattatgact gtatcagaaa atatgatacc aaacggatct
    13381 attcattaat taattgacat agtttatctg ttgctctttt atgactggat aactgaccaa
    13441 ttcatgacga tccaagtctt ctttattcat atttacagca gtaatttgac tgtcttcata
    13501 cgtacgataa taataaatcc ctttatcaac gttacagcaa gaagaataaa tcgtatattc
    13561 gtattttctt tctccagtat cgcatactcc tttttgttgc tcgactgagc ctagtatatg
    13621 aaaaaattga ctgatacttt cagattctga atcacctgat gcagcattca ttttagtaaa
    13681 tgtggctttt acaaatcgag aaacagaaga gagatctcca ggtaagccca atcctcccat
    13741 cccacgacta taaacattca agctgatctg gtttgaaaaa tgattttctg gtgttccatt
    13801 tgatagcgag cgataattgt tcagattaaa taattgataa tcaaatggcg gattatttgt
    13861 caacacaccg acaggattat catataaatg aagtccatct ttcatacttt caattactat
    13921 cgatttttct ttatcagcta ataaccagtg taaaggtgac aagggtagtt tttcactaaa
    13981 atcgatattg actagattga tagtagccaa taattcttta gcttcgtcta tggttgagca
    14041 ttgccctagt atccatggaa taaactcaaa tggtgcaacg ttatcttttc cttcttgtaa
    14101 ttctttataa tcagcattgc caggaaaatt cagtcctgcc atactcaacc ctttttcatt
    14161 tgtagcctca taatagagtg gatagttgga taccccagct gctattccta tcattgcata
    14221 atgtttatct aaatttttta cctttctaaa atcgaaacga taatttcttg gagtaatagt
    14281 gaccacttca ttataagaca tctcataatc aaaattcctt ccaaaataat ggtctttcgt
    14341 tgcataagtg attgcggtac acatcgtttc ccgtcctaaa acaaatcgtt tttacacttt
    14401 tatcttacat ctagtatttt ttttatcaaa aaaaggccaa agaagattga tttactagca
    14461 tttataaaaa atagtactca gggggaatca aaaaatattg taggttaaaa gtacaacaat
    14521 aaggggctgt gacaaaagtc ttgtcacagc cccttattcc gaacaaacgg tgttcaaaag
    14581 cttgcatctg cggtaataag cccaatcaaa cgaaaaaata ggaaaagcta tttttgtttg
    14641 gccgttctta ttacttgatg caggacagct tcttcacaac ctctaatcgt tgatataagg
    14701 aaggtacagg atttgaacct gcgcgccggt attagccggt tcgccggatt tcgagaatag
    14761 ggtttaaagc tgattttaag tgtttttcgc ttattatcag ctttttatta tctaaaaaaa
    14821 caacttttag agacatccat atactgcttg ggggagtcaa aaggggagtc atttttatat
    14881 tttattcgca ttttaaatca cttagagtat gtaccctgta ggactcgaac ctacgaccgg
    14941 acggttatga gccgtctgct ctaaccaact gagctaaggg tacggaaagc cataaacagt
    15001 caaccagtag aatgtgtggc aacaaacctg ttatcgcata tcttggagtg tgactattta
    15061 tgagtgatag tgaagatatg cggcaacatc actattttat cgaatgattt ttatagtttt
    15121 caatatagtt atgtaccgcc cctc
//
LOCUS       DAGCYL010000058        15106 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.059, whole genome shotgun sequence.
ACCESSION   DAGCYL010000058 DAGCYL010000000
VERSION     DAGCYL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.059"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(21..251)
                     /locus_tag="MB216_001348"
     CDS             complement(21..251)
                     /locus_tag="MB216_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526190.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="MB216_001349"
     CDS             complement(687..1253)
                     /locus_tag="MB216_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBT4526191.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="MB216_001350"
     CDS             complement(1253..2209)
                     /locus_tag="MB216_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBT4526192.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="MB216_001351"
     CDS             complement(2209..2865)
                     /locus_tag="MB216_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBT4526193.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="MB216_001352"
     CDS             3235..5253
                     /locus_tag="MB216_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4526194.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /locus_tag="MB216_001353"
     CDS             5680..8094
                     /locus_tag="MB216_001353"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBT4526195.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="MB216_001354"
     CDS             8442..9995
                     /locus_tag="MB216_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBT4526196.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="MB216_001355"
     CDS             complement(10376..12160)
                     /locus_tag="MB216_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4526197.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="MB216_001356"
     CDS             complement(12175..13896)
                     /locus_tag="MB216_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4526198.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="MB216_001357"
     CDS             13996..14757
                     /locus_tag="MB216_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4526199.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4911 a         2771 c         2978 g         4446 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaata
//
LOCUS       DAGCYL010000059        14998 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.060, whole genome shotgun sequence.
ACCESSION   DAGCYL010000059 DAGCYL010000000
VERSION     DAGCYL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.060"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            162..803
                     /locus_tag="MB216_001358"
     CDS             162..803
                     /locus_tag="MB216_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4526200.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            846..1538
                     /locus_tag="MB216_001359"
     CDS             846..1538
                     /locus_tag="MB216_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBT4526201.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMEQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQDFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            1535..2437
                     /locus_tag="MB216_001360"
     CDS             1535..2437
                     /locus_tag="MB216_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4526202.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQLG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     LDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2595..4301)
                     /gene="efbA"
                     /locus_tag="MB216_001361"
     CDS             complement(2595..4301)
                     /gene="efbA"
                     /locus_tag="MB216_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBT4526203.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIAELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFHRLSQTELEPKAIQRQFQGIGFDTAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKNFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRDGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTEYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            4491..5114
                     /locus_tag="MB216_001362"
     CDS             4491..5114
                     /locus_tag="MB216_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526204.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIRQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            5564..6613
                     /locus_tag="MB216_001363"
     CDS             5564..6613
                     /locus_tag="MB216_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4526205.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDVLIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVA
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            6610..7491
                     /locus_tag="MB216_001364"
     CDS             6610..7491
                     /locus_tag="MB216_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4526206.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            7494..8264
                     /locus_tag="MB216_001365"
     CDS             7494..8264
                     /locus_tag="MB216_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4526207.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCKEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            8543..9397
                     /gene="aroE"
                     /locus_tag="MB216_001366"
     CDS             8543..9397
                     /gene="aroE"
                     /locus_tag="MB216_001366"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBT4526208.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            9575..10738
                     /locus_tag="MB216_001367"
     CDS             9575..10738
                     /locus_tag="MB216_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBT4526209.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            10855..11265
                     /locus_tag="MB216_001368"
     CDS             10855..11265
                     /locus_tag="MB216_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4526210.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            11439..13667
                     /gene="topB"
                     /locus_tag="MB216_001369"
     CDS             11439..13667
                     /gene="topB"
                     /locus_tag="MB216_001369"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBT4526211.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            13838..14122
                     /locus_tag="MB216_001370"
     CDS             13838..14122
                     /locus_tag="MB216_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526212.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            14282..14518
                     /locus_tag="MB216_001371"
     CDS             14282..14518
                     /locus_tag="MB216_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBT4526213.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            14515..14913
                     /locus_tag="MB216_001372"
     CDS             14515..14913
                     /locus_tag="MB216_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBT4526214.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         5038 a         2531 c         3182 g         4247 t
ORIGIN      
        1 gaagagaata atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa
       61 gggagctgtt tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat
      121 atgatattcg gaaaaagaaa tctcaaaggg aggtaacttt tatgggattg aatgaattta
      181 ttttaggatt gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag
      241 agcatacagt tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca
      301 ttgaagaagc gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc
      361 atgactacac agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag
      421 agttaagagg gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag
      481 agattccagc tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa
      541 ccgtagaagg agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg
      601 atgaaatcat caaaaataac ccggaaaaag tttataagga gatgtttatt gaggcagtga
      661 ctacgattaa aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc
      721 cggaactatt gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga
      781 ttgtaaagaa agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa
      841 agaatatgga tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt
      901 tatgtgattg gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca
      961 agggggaaga aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt
     1021 ttactgacaa ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg
     1081 tagagttcaa gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac
     1141 atatcatcaa cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa
     1201 aagagaatct agtcgtgggt gtcatgtacc agctaatgga acaggaaggt tggctgtata
     1261 atcaagaaaa tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg
     1321 tgttctttcc ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta
     1381 ccagcgggcc aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagattttc
     1441 ttctcccctt cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg
     1501 gaagagaaat ttattatttc gaggagtttg agcaatgaat atccagcaga tgaaatacgt
     1561 ggtcgctgta gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca
     1621 gccaagtctt tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac
     1681 tcgaacgaat cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga
     1741 gaaaatattg gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga
     1801 caatttttcg atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact
     1861 cgaggaattc aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt
     1921 gatcgaagaa gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa
     1981 tcaagctggt atcagaagat atttacaact aggagagcta agtggccgca gcattggaaa
     2041 attcaaaacg catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac
     2101 caaagaagaa ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc
     2161 gtatttttat gaagacctta tcgagatcct tgatcaagaa acagtgattt atacgagtga
     2221 tcgaggtact ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat
     2281 tgtgattgga gggattcgcg agcatttgcg gttaatccct ttggcagaag gcgaactaaa
     2341 tgaattttat atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat
     2401 caaagagctt actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt
     2461 tattgaacac cgttaatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt
     2521 tttgtagcaa acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta
     2581 gaaacactca ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc
     2641 acgataatcg tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt
     2701 cggatatgct ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa
     2761 aaataggcag ctaactcggc agcttctgta attgtttcat cggagggctt gtcactttta
     2821 atgatgacgt gcgaaccggg tatatttttt gcatgaagcc aatattcggt ttttttagct
     2881 gtcttcattg tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg
     2941 tcagaagaaa agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc
     3001 ttctttttca ggtatccttc tgaagtcaat tcttctttga ttgcttcgat atccattggg
     3061 ccggcaattt ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct
     3121 tggatttgtt cgccaatcag cttaacagcg ttcttcagct tttggtaacg atggaaatat
     3181 ttctgtgcat tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct
     3241 tcttcataat aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa
     3301 gtagtaagta gttcgccatc tctacggtaa ttctcagcgt tttcggattc tttgagtgtt
     3361 tgttcacgtt ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc
     3421 agttctccgc cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct
     3481 aataattgac tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga
     3541 taagcaatcg gtgtgaaaaa atttttattt tccgtttcgt aaaaagtagg aataggctga
     3601 tggctgatgg ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt
     3661 aatcttttgg tcagctcttg agcggtatcg aaaccgattc cctgaaactg acgttggatt
     3721 gcttttggct ctagttcagt ttgggaaagc cgatgaaaga ttttttcttt ctcggagcta
     3781 aatgggttca agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa
     3841 cgataagtgt tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct
     3901 cgattaacta agacgatcgt actatgacgt cccattaatt cagcgatcaa tatgatattt
     3961 tgtagatcac ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg
     4021 atttgttcga tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag
     4081 ttaggcggat tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt
     4141 gggtgcgcag ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt
     4201 tcattttcat aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat
     4261 tcgttgacca tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt
     4321 ttcataagta tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa
     4381 agtaaaagtc tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa
     4441 atccaaattt ttaggtataa taaaaaatag gagaaaaagt agaaaagaag gtgtttgtta
     4501 tttgggaagt agttaaatcg agagtacaag aaatgaatga acatttctca gtgtcattcg
     4561 cagaaaatcc aagtaagtat tttcgacgtc ttgtgtttct gctcatctta tcattgctgt
     4621 taacagttgc aatgttttta ttgccggaaa aaatccgaga agagcaagta aatagtctcg
     4681 tttattcgtc cataccaacc agcaaattga aaccttttga ttacagcaaa gctgatcaga
     4741 aaatcagaca aagccgggcg atatcggtga tgttcagcaa accgaacggg aaaacctatc
     4801 aggacatgct tgatgttttt aatgatcctg agcttatgga agaattgaat cgaccgatct
     4861 tttattatcc gatcgtttat gatgtacatg aactggaaca aaaatataac atacgaacgg
     4921 atgaagtgac atttattttt tttgatggag gaaaagaagc aaatcggata actgcgggta
     4981 agggaggaat aactgatttt gataaggagt tgatccctga actgaatcga cttccgttag
     5041 ccaatatcaa acaattagaa gaagaattgg atagaacggg agtaacttcc caaaattccg
     5101 agattgctaa ttgagacatt ggcgcgatga aacggtgggt ctatatagcg ctatttttaa
     5161 ttatcagaac aagtgatgtt tgatcttcaa aataacaaat aaaaaattaa aaaatatagt
     5221 tgacgaaatt caaaaattcg attagactta aatcaattct taaggaaagg gtgacctgta
     5281 atgaacttat caaaacgtat ttcagcagaa aacaaaaaaa attggcgcag ctggcgtaga
     5341 taagttgtat taatggtgag actttcccat agatacaact ttttagagtg catttctaaa
     5401 gcttgtatct atgctagaca tgaacattga ctgccgcata gattagaatc tatgcggttt
     5461 tttgtgtaaa gacaatgacc gctttggcca ttgtcttttt ttgtagactg gatacttgcc
     5521 taattataat aaataaaaag attgactttg caggaggaaa aaaatgaaaa acaaacgatt
     5581 aataacagtt gtagtaatta ttttagttta tttagcaggc gcatttattt atggaaaagc
     5641 ctcaggcgaa aaagaaagta cttcgacgaa ccagacaaaa aaaacaacag ttgttggggt
     5701 attgcaatac gtgagccatc cggcattaga tgagatttat cgaggaatca aagatggtct
     5761 gaaagaagaa ggattaaaag aaggagaaaa tcttgagatc aaatttcaaa atggacaagc
     5821 cgatcagagc aagctagcca caatgagtca acagctagta caatctgatc cagatgtctt
     5881 gattggaatc gcaacacctg ccgctcaatc attagcaaac acgacttcat cgatcccagt
     5941 tgtgttaggt gctgtaacgg atcctgtggg ggctggatta gttgcttctt tagagaaacc
     6001 tggtggtaat gtaacaggtg tttctgatca gccgcctgtt gcttcacaaa tcaagcttgc
     6061 ttctgatttg ttgccaaatg caaaaaaagt cggaatcttg tatgcttctt cagaggataa
     6121 ttcaaaatac caagtagcac aagcagaaga agctgcaaaa aaagaagggt tagaatcagt
     6181 aaaatatgcg gtgccttcca ctaatgagat tgcacaaacg gttcaagtga tgagccgaca
     6241 agtcgatttt atctatgttc cattagataa taccattgcc aatgcgatgc aaacagtggt
     6301 aaaagaagca aacaaagcaa atatccccgt cattccatct gtggatacaa tggtggaaca
     6361 aggtggacta gcaacaatcg ggatcaatca atatcaatta gggcttcaaa gcggtaagat
     6421 ggcagcagaa cttgcgagtg gaaaagaaaa accggaaacg acgcctgttt atacatttga
     6481 tcaaggggat acagtgatca atcaatctca agctgatcgc ttaggaatca ttgttcccca
     6541 atcgatgaag gaaaaagcca aaatcattac agatgactcg cagcaagaaa catctaagga
     6601 ggatgacaaa tgatcgtatc agctatttca caaggcatgc tttgggcaat attaggatta
     6661 gggattttca tgacttatcg gatcttagac tttcctgata tgacgacaga agggtccttc
     6721 ccattaggtg ctgctgtctg tgtgacagct attactcatg gggtagcgcc gattgtagct
     6781 acattattag gagtaggtgc tggcatgata gcaggactcg ttacggggtt actttataca
     6841 aaaggaaaga taccggttat tttagcaggg attttagtga tgtcaggatt gaactcagtt
     6901 attttgtttg tgatgcgaac accaaatctt tctttattga atcatccgat tctacaagac
     6961 agcttccatc gctttgggtt acctgattac tttgacacag tccttcttgg cttcatctct
     7021 ttgggcatct tgattgctgt actgctattc ttcttcaata cagatttggg tcaaggctat
     7081 attgcgacag gagacaacga aacgatggct cggtcgttag ggataaaaac cgatcgtatg
     7141 aaaattttag gtttaacttt gtcgaacggt gtcattgcat tatccggtgc gctgattgcc
     7201 cagaacgacg gatatgcaga tgtaaataaa gggattggcg taatcgtcat cggtcttgct
     7261 tccatcatca tcggtgaagt gatcttccaa gaattgacgc tagcagaaag attgattgct
     7321 attgttgtcg gcagtatcat ttatcagcta ttgattttag tagtaatcaa actagggttt
     7381 gacacgactt atctgaagtt gttctccgct atcatcttag ctatttgtct gatgatcccg
     7441 caattgaaga aagcactgaa cttaaaaacg ggttttgaga aggaggcata gtaatgactg
     7501 atatattgac aatcaaaaat ggcagaaaag tcgtaaacaa tggaatgaat gaagagaaag
     7561 tactattgga tgaaattaac cttacaatca gagaagggga cttcatcact gtccttggcg
     7621 ggaatggtgc tggaaaaagc acactgttca atacgatcgc tggtacgctt caactcagtc
     7681 aaggatcgat ctcttttaaa gatcagctga tcacaaaaga ttcagaagaa aaaagagcac
     7741 gattcctttc tcgagttttc caagacccca agatgggaac ggctcctcgg atgacagtag
     7801 cagaaaattt gctacttgca caaaaaagag gtcaaaaacg gaaattccgt ttacgtaact
     7861 tgaaaaatca aaaagaagat tttttcagac tatgtaaaga agtcggtaat ggcctagagc
     7921 aacacttgga tactccagcg ggtgaattat ctggtggtca acgccaagca ttaagcttgc
     7981 tgatggcgac gatcacagct cctgagttac tattattaga tgaacatact gcagctttag
     8041 atcctaaaac agctaaagca ttaatgcgat taacaaatca gcggatcaat gaacaaaaat
     8101 tgacttgtct catgatcacg catcgaatgg aagacgcctt gaaatacgga aatcgtctga
     8161 tcgtactcca aaaagggaaa atcgttcaag atatttctga aaaagaaaag caacatttga
     8221 caatgggcga ccttttgcag ttctttgaag aagtagtgga ttgatttcga cagaaaaatt
     8281 cctttcagta gaaggacttg aaaactcatt gacatcttcc tttgattcat cctatactta
     8341 aaacaataaa caattaaaca tatttgggtg ttttgtagga tcacgtagtc aatgatttta
     8401 acaattaaat agaatattct ttgctctgac aagtaaacga tgagaaaaga atttgctact
     8461 ttgccaccta gacgtttcgt actttttcgt atcaaacgct aagtggcttt ttttattgaa
     8521 gtaagaacaa aggaggaata agatgatttc tggacaaacc aaattagctg ggctctttgc
     8581 tgatccggct tctcacagtt tatccccatt gatgcacaat actgcttttg aagcaaacaa
     8641 catcgatgcg gtgtatttag ctttccaagt aaatccaatg aatttgaagc aagcagtcga
     8701 aagtattcga acttttgata tgttaggtgt aaatctttcg atgccgaata aaacagcggt
     8761 tattccttat ctggatgagg taagccagga agcgcaattg atcggagcag tgaatacgat
     8821 cgtgcacgaa aagggtcgtt tgattggcta taataccgat ggtgcgggct tcatgcaatc
     8881 cgtgcaagaa gctgggattt cgatcaaaaa gaaaaaaatg actctactgg gagctggagg
     8941 tgcagcaaag gcagtcactg ttcaagctgc gttagacggc gctgaggaaa tcgtagttta
     9001 taaacgaaac aaccaaacat tcgctcaagt aaaagaaaca ttcgaacaac tcagctatcg
     9061 aacttcttgt aagataaaag ttcgtgactt tgccgatacg catcaattaa gaaaagactt
     9121 gaaagaaagc tgtcttctag tcaatgcaac agatatcggg atgggaaaga aaatcaatca
     9181 gtcaccgctc ttagatataa gcatgatgca tgagaatcta gcggtttttg atttgattta
     9241 tgcacctaga gaaacacgtt tattaaaaga agcaaaaaaa gtaggagcaa aaggatacaa
     9301 tggacttggc atgttgatct accaaggagc aatcgcgttt gaattatgga caaaggaaaa
     9361 gatgccgatc catgtgattg aaaagctgtt tgattaaaaa aggataatag ataaagatct
     9421 tttattctcg cataaacggt ctcaagaaaa gtaatccttt atgtatcgaa gcatttattt
     9481 cgttattttt tattaaatta tcaaaaaatg ataggctttt gtcgattggt tatcgtatcc
     9541 tttatttatc agtaagaaac gaggaggaac cacaatgagt cggatggatc gctataagga
     9601 catccataag aaggcgaaac ctctgaagcg ggatacgggg agtatttttg ctagaagaga
     9661 acgaaaacga acgcgtatgg aaaatgagga agatgctacc cgtatttatg aaagagagca
     9721 gcattcattt gaacatttga accaatacga gcaacagata tacagagatg caccaagagc
     9781 agaagaacaa caaaaaaata aaaaattttt caatcggcaa aagaagaaca aaccgttaaa
     9841 acctcgaaga aagcgatcgt ggaaaaaaat cattctatcg attttgcttg ttttgatcct
     9901 ttattcaggt atctcatttt ttattggtaa atatgtagct gaacatgaca caagcttaga
     9961 ggcagcgact tcagaaacct ttcatgggca aaaagcgacg aatggtgcgc ataatatttt
    10021 gatccttgga agtgatacac gcggagaaga tgccgggcga gccgatacga tcatggtctt
    10081 acagttggat ggtccttccc ataagccgaa actgatttct tttatgcgtg acagcttcgt
    10141 tacgatccca ggggtaggac aaaataagat caattcagct tatgcatacg gaggagccga
    10201 tctagtgcgt caaactttag tagaaaattt tggaatcgat tgtcaatact atgcgaaagt
    10261 cgatttcaaa tcgtttgaaa aagtgatcga tgccttattc atgaacggag tgaagatcga
    10321 tgcagaaaaa gatctgaatc tggatggtgt agatattaaa aaaggggtcc agaagatgga
    10381 tggacatgtt cttttgcagt acgcacgttt ccgaatggat gaacaaggag actttggccg
    10441 tgtcagacgc caacagcaag taatgaacgc tattttcagc caattgaaga atccattgaa
    10501 tctgatccgt gcaccttatg cagctggtaa agctttagga tatacatcca cagatgtcac
    10561 tgcttggttc ttgacgaaaa acctcttttc gattgcacga ggagctggtg gcattgatcg
    10621 aataagtgtg ccagtagacg gttcgtggaa ttatgggcaa agtgattatg ctggcagtat
    10681 tcttgaaatt gataatcaaa tgaatcaatc tgctattaca gattttttga ataaatgatc
    10741 ataaaaaaga tcaagaagaa gctgcgcaag gcttttgtca tgaatcaatc aaacgattca
    10801 ctcgtaaaaa acgctttctt atggtagatt taaccaagag aggagctgtt tacattggat
    10861 atctatctga acttcaaaaa tgagtcgcgt gaagccatcg ctttttatga aaaagttttt
    10921 cagactgaag catcaggtat caccacatac ggtgaaatgc ctggaataga acaaatgaac
    10981 ttgccagaag aagcaaaaaa tctaatcacg aatgcacggc tgaacattta tgatacatta
    11041 gttatgtttg cagatacgcc agaaggaatt ggtcctgaat tagttgtggg aaataatgtt
    11101 tctttagtca tccagacaag aaatgaagaa gaaacgaagc gcctatttga aagcttagct
    11161 gagggtggta agattgagat gccactggat gctgttccgt gggctcagtt gtatgggatg
    11221 gtaactgatc gatttggcat caactggcag attaaccaag agtgacagct aaagatgaat
    11281 attttctgga aacatcaagc agcaaacgga tcatcgtttg tttgcttgat gtttttgtgt
    11341 ctatttcgac tatacggtat ctttttttta ttgccagatg cgaaaatatg ttccataata
    11401 gagaagtccg caaaaaatta agtaacggag gtcagaagat gaagacggtg atactagctg
    11461 aaaaaccttc acaagcaaaa gcctatgccg aaaccttcca acagcaaaag cggcatgaag
    11521 ggtattatga aatcagcgat ccattgtttg ctggtgaagt aaccatcact tatggttttg
    11581 gacatttggt agatatggtt gctccaggag cttatgaaga gcgttgggca aaatggtctt
    11641 tagaaaattt gcctattttc ccagaaacct tccgttacga agttccaatg gacaaacgtg
    11701 cacaattttc gatcgtaaaa cgagaattgc agtcagccga taccatcatt attgcaacag
    11761 atggtgatcg agaaggtgaa gccatcgcgt ggtcgattat tcgacaagca aacgcatttt
    11821 ctaaggaaaa gaactattta cgtttatgga tcaattctct tgaaaaagaa gcaatctatg
    11881 aaggttttaa gaatctccaa cctggaaaga actattttcc aaaatataaa gaagcgcaag
    11941 caaggcagaa cgctgattgg ctgattggaa tgaatggaag tccattatat agcttgctac
    12001 ttcaacgaaa agggattgat ggcagttttt ctcttggtcg tgtacaaaca ccgacactct
    12061 atatgattta tcagctgcaa gaagaaatca aacacttcaa aaaagaaacg tactttgaag
    12121 gagaaggttt aattacgaca aacaaagggc agttttctgg aaaaatcgta ccaaaaaaag
    12181 catttaaaac ccaagaagag ttaacgaagg aaatagaaaa acttggagcg catttaggta
    12241 agcaaaacgg acggattctt gaagttacga aaaaggaaaa gaggttgcat agtccacgtt
    12301 tattttcgct atctagtttg cagacaaaga tgaatcagct aatgaaagca agtgccaaag
    12361 ctacacttga tgctgctcaa ggattgtacg aaagcaagtt tctaagctat ccaagaacgg
    12421 attcctttta cattacagaa aatgagcatc agtatctggt taaacatctg caaaattata
    12481 aaaaattttt aggaatagaa gaagtacaga caactgagat caaagcaaag aaacgatatg
    12541 tcgatgcgaa aaaagttcaa gaacatcatg caattattcc gacaaagacg attccgactc
    12601 ctgcacgctt tgcgaagctt tccaaattac agcaagcaat ctatttgcaa gttttgcgca
    12661 ctactgtggc tatgtttgct gcagattata tctacgaaga gacgaccgta atgacgggcg
    12721 tacagcaatt aaagctaaaa agcatcggaa agatcccatt acagcaaggt tggcaggaaa
    12781 tcttacgcat caagtcaaaa aagaaagaag ctcagactct tcctcctatc gtagaaaatg
    12841 aagaagcaga agtagaactg aaaagtatgg agaaagaaac acaacctcca aaaccctata
    12901 ccgaaggaac gttgatcaca gcaatgaaaa ctgcgggaaa aacgctggat aatgatgaag
    12961 cgcaagaaat tctcaaagaa gtagaaggaa tagggacaga agctacacga gccaatatca
    13021 ttgaaaatct taagcagaag cattacatcg aagtcaataa aaacgaaata actgttacgc
    13081 caaaaggaat cactctttgt aaggcagtag ccaatgaacc attactgacg agcgcagaaa
    13141 tgactgcgcg ctgggaaggg tacttgagaa aaattggtaa acaagagggg acaccatctg
    13201 tttttttaga gaatatcaaa aaattcatcc tacatttatt ggcagaagtt cctaatcaaa
    13261 tcgacaaggt agacttttca gatgagatta gcgggacacg tcagataaaa gcgatggaga
    13321 agaaaaacga tcaattagga agatgtccaa aatgtaaaaa gggaatagtc atgctttatc
    13381 ctaaaatcgc tacatgcttg aatccggaat gcgacttcaa attgtggcca acagtagcta
    13441 aaaagaagct gactaaaacg aatctcagag atttgctttc aaaaggaaag acaagtaaag
    13501 ttgtcaaagg ttttactggt aaaaaaggga aattcgatgc agttttagta ttaaaagaag
    13561 atatgacaat cggtttttct tttccatgga tcgaaaacag tggaacaaaa gcagttgatg
    13621 gaaacaaaga aacatcaaga aaaaataatg aaaacgaagt agagtaaatg aaaacaatga
    13681 aatagcggaa tatttattcc gaaaagtgag aatataaata ccaataaaac taaaaaatat
    13741 cagtgaatca agcaaaaacg ttggggtagg ctgattttat atagatagca aggagttttt
    13801 ttatttgaaa aaagtgcatt catttttaaa ttatcttatg ggagcaatca cgattgtggc
    13861 tgtgtaccaa gcatttctaa agtatcctga ttacgggacg aagttgttgt cattgacgca
    13921 caatcaaaca gcatattcac caacgttgat tttatcgact ttgactgcag gagctaaaag
    13981 cgctacttat ttttttcata aaacgacaag catgctaaag cgaaagaaac agcgaaaaca
    14041 attgttggtg gtggtagcgg ctatgatcat tatttatttt ctaccagtta ttacacaagt
    14101 atttcatcaa tattttggtt aagaatacga gagtttgtga caaaagtcat gtcacaaact
    14161 tttttctttg tcattttttc gtaagaaaaa gcaaaataaa agtatataca ttgattaaaa
    14221 gtatatactt ggatataatg tacttactat agaatgagaa tggtatagag ggtggcgaaa
    14281 tatgaaaaaa atgaaaaagt ataaaattag aaaatctggc aatagcgatg taacaactat
    14341 cccaccagaa gtaaaagaat tcatgggtgt gcagacaggt gacgcaatca gttatgtttt
    14401 tcaatctgat ggttctgtac gaatgatcaa agcacaagaa gaacctgata ttgattcatt
    14461 agttgattcg atcatgaatc aatatgaaga cgcgttaaaa gatttggttg atttatgagg
    14521 tatctttcga ctaaagaaat tatcaaaatc aacgcgaaag ttattttacg tcactcgcca
    14581 ggcgaaatga tcggtgtaaa agatgcaaat gcattagata tggccgtcaa acagccttca
    14641 caagcagtat tcaatcaaga actctatcct gaaatctatg aaaaagcggc gattttagca
    14701 attaatttag ctaaaaaaca tccttttcat aatggcaata aacgaactgc tcttgttgcc
    14761 atgttgttgt ttcttcaatt gaataattgt ccagttgctt tttctcgtca agaagctgtt
    14821 gattttatca taatgattac cactagttca ttggatttcg attcactaaa gtccaagata
    14881 accagttatt tacgacaaac cgcaataaaa tgaacgaatt aaaaacttta tttctcaatt
    14941 agatcagcat gtcattaagg aatatgctga tttttttatg tttaatcata caaaatag
//
LOCUS       DAGCYL010000060        14922 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.061, whole genome shotgun sequence.
ACCESSION   DAGCYL010000060 DAGCYL010000000
VERSION     DAGCYL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.061"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..501
                     /locus_tag="MB216_001373"
     CDS             <1..501
                     /locus_tag="MB216_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4526215.1"
                     /translation="LCPNNQPLLFSTITRDGYRTYKSNPKFCRSCPLLKNCTLSTKHQ
                     KIVTRHVWADLMDELEHQRHTALNREIYKKRKQTIERIFADAKEKHGMRWTKYRGLEK
                     VATHTMLTFAAMNLKKLATWLWKAKKPMAFYLKFLQRLTKRSWQLSHSFLSTVWELYF
                     DYSFFF"
     gene            590..976
                     /locus_tag="MB216_001374"
     CDS             590..976
                     /locus_tag="MB216_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526216.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINDEKRSVLSHEKKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            963..1661
                     /locus_tag="MB216_001375"
     CDS             963..1661
                     /locus_tag="MB216_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBT4526217.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            1666..2130
                     /locus_tag="MB216_001376"
     CDS             1666..2130
                     /locus_tag="MB216_001376"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBT4526218.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLVLAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICILIYIILDEKDQRKK"
     gene            2127..3038
                     /locus_tag="MB216_001377"
     CDS             2127..3038
                     /locus_tag="MB216_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBT4526219.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKPAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            3273..3812
                     /gene="pyrR"
                     /locus_tag="MB216_001378"
     CDS             3273..3812
                     /gene="pyrR"
                     /locus_tag="MB216_001378"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBT4526220.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            3826..5109
                     /locus_tag="MB216_001379"
     CDS             3826..5109
                     /locus_tag="MB216_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBT4526221.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            5122..6048
                     /locus_tag="MB216_001380"
     CDS             5122..6048
                     /locus_tag="MB216_001380"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBT4526222.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            6067..7347
                     /locus_tag="MB216_001381"
     CDS             6067..7347
                     /locus_tag="MB216_001381"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBT4526223.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            7352..8428
                     /gene="carA"
                     /locus_tag="MB216_001382"
     CDS             7352..8428
                     /gene="carA"
                     /locus_tag="MB216_001382"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBT4526224.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            8433..11618
                     /gene="carB"
                     /locus_tag="MB216_001383"
     CDS             8433..11618
                     /gene="carB"
                     /locus_tag="MB216_001383"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBT4526225.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTYHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            11672..12448
                     /locus_tag="MB216_001384"
     CDS             11672..12448
                     /locus_tag="MB216_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBT4526226.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            12445..13377
                     /locus_tag="MB216_001385"
     CDS             12445..13377
                     /locus_tag="MB216_001385"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBT4526227.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDPDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            13374..14090
                     /gene="pyrF"
                     /locus_tag="MB216_001386"
     CDS             13374..14090
                     /gene="pyrF"
                     /locus_tag="MB216_001386"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBT4526228.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKEIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            14097..14726
                     /locus_tag="MB216_001387"
     CDS             14097..14726
                     /locus_tag="MB216_001387"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBT4526229.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            14714..>14922
                     /locus_tag="MB216_001388"
     CDS             14714..>14922
                     /locus_tag="MB216_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526230.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISS"
BASE COUNT         4817 a         2669 c         3397 g         4039 t
ORIGIN      
        1 ttatgtccca ataatcaacc gttgttattt tcaactatca ctcgtgatgg ttatcgaacc
       61 tataaatcaa atccaaaatt ctgtaggagt tgtccattac ttaaaaactg tactttgagt
      121 acaaaacatc aaaaaatagt aactcgacat gtttgggcgg atcttatgga tgaacttgaa
      181 caccaacgcc atacggcgtt gaatagagaa atttataaaa agagaaaaca aaccattgaa
      241 cgaatatttg cagatgcaaa agaaaagcat ggtatgcgtt ggacgaaata tcgaggtctc
      301 gaaaaagtcg ccacgcacac gatgcttacg tttgctgcca tgaatttaaa aaaactagcc
      361 acatggctat ggaaagcaaa aaagcctatg gctttttatt taaaatttct acaaagacta
      421 acaaaaagga gttggcaatt aagccactcc tttttgtcta cagtctggga gctgtatttt
      481 gactacagct tcttttttta gacgataggg aattaatttt gtttacacta ggatcattct
      541 ttttctttat tgtattttga gctatactaa agcataatag gagggtatga tgaaaacgac
      601 atttataaga aaaaccggtt tttacggtat gggtagctcg attcagctaa gaatcaacga
      661 tgaaaaacgc agtgttctca gtcatgaaaa aaagataacg cttgatctgg aagtgccctt
      721 tacgatgcag gtcacctttt attggctaaa aagccccgtt tatacagtga aagaagcaaa
      781 gcctgcttat gtgatcacaa tgaattactt gattcttcaa atctatccgt ttttatttct
      841 agcaactggg ttgggggccg ttttcctaca aaatttattt ttcagttttt ttgcagttat
      901 tgtgttgatt gcgttctttt tctatataaa aaaccgggca tatttgatca aggaggaaaa
      961 ttatggcaaa ctctgatctt tttttaatca gtttcaatcg aatcgaaaaa tggatgagag
     1021 acgagatggg aaatgcacga aatatgggat ttaccgaact tgtcagacgc ctagctcaaa
     1081 gaaaacaatt aatgatcaga aaatacgaag atgatctttt acagcttgct cagttaagga
     1141 atgcgatcgt ccatgatcgg atcgcagtcg attttgtcat tgcagagcca aatgattgga
     1201 cagtcaagcg aatccagctg atcgaacaag aactgattca gcctgaaacc gtgctgcctc
     1261 gatttgcaaa acgtgtaaca ggttttgaac aagatcttcc gttacttgaa ctgttaaaga
     1321 tagtagcaga aaaaaggtat tcacaatttc cgttatacaa taaaggaaaa tttgtagcat
     1381 tgattacttt acgtaatatc gggttttggc tagcaaaaga aagccaaaaa ggtccggttg
     1441 atttaacaaa taaacgagcg ttggatttga tcatccaaaa cggaaagtac acaaattatc
     1501 attttgttcc agctaaaaca catatttacg aggttgaagc gatgtttcgt gagcaaggga
     1561 ctttggaagc cgtacttatc actaaagatg ggaatcccga tggtaatctg ctcgggatcg
     1621 tacgacctcg tgatatttat aaacaaatag aaaaggattg attttttgtt aattatttat
     1681 tggatattca gcgctttgct agtaggagtg gatcagttgg tcaaatggtg gatcactgat
     1741 aatctagcgc ttggagagac gaaaaatctt ataccaggta ttttgtcact gaatcatatt
     1801 cgaaacactg gagccgcatg gagtatgcta gaagggaaga tgtggttctt tactatagtg
     1861 acactcattg cagtcgttgt gattctcaca ttgatgatta aaaatcgtga aaaaggtaat
     1921 cgctggttca tgattggatt gagtttggtt ctagctggtg cgattggcaa ttttatcgat
     1981 cgtattcgtc ttggctatgt agtagatatg ttccagacag attttatgag ctttccgatt
     2041 tttaacgtag cagatgtgac acttgtatgt ggagtgattt gtatattaat ttatattatt
     2101 ttagatgaaa aggatcaaag aaaaaaatga cagaacttaa aacgacgatt caccatgaat
     2161 ctggtaggat cgataaagta ttgactaatt tattcaccga ttacagtcgt tcgcaaattc
     2221 agctgtggct gaaaaatgga gcggtttctg tcaatggaca gccagtcaaa gcaaattaca
     2281 aagtaaaaca aggagacgag attactgtct tgatccccga accagaaaca ttagatgtgg
     2341 tcccagaaaa tatcccgttg acgatcgttt acgaagatga agatgtagcg gtgataaaca
     2401 aacctcaagg aatggtcgtt catccttcag caggtcatac gcatggcaca atggtcaatg
     2461 cgctgatgta ccatatcaag gatctttcta cgatcaacgg cgttattcgt ccaggtattg
     2521 ttcaccgaat cgacaaagat acttccggac tgctaatggt tgccaaaaat gaccaggcac
     2581 acgaatcatt agctcagcaa ttaaaagata agacctcttt gcgcaaatat gttgctttag
     2641 tccatggaga gattcctcac gaaaaaggaa ccatcaatgc gccaatcggc cgttcgaagg
     2701 aaaaccggaa aatgcaggcg ataagagaag atgggaagcc ggctgtcaca cattttactg
     2761 tattggaacg tttcgaagga tttactctgg ttgaattaaa actagaaacc ggaagaacac
     2821 accagatccg tgtgcatatg aagtatatcg gttatccgtt agctggagat ccagtatacg
     2881 gaccgaagaa aactttgaaa ggcaatggtc agtttctcca tgcgcaaatg ttaggtttta
     2941 cgcacccgac tacaaacgaa aagatgatct ttgaagcacc tcttccagaa gtttttgaaa
     3001 aaactttaga aaaattaaga aaaaacattg cattttaatt tagataacgg tatagtaatt
     3061 aacaacgaaa gcataaaact taatagaatt cctttaagtt tagtcccgtg aggctaagaa
     3121 gggtgggagc aaggatacgt atggtgcgcc ataagtagat tgctcattga catagtatag
     3181 ctatcagtgt atcagtatac gctcagtcta aacattcctc ctgtcctctt agggcaggag
     3241 tttttttatg caaaaaagta gggggaatga atatgcaagc aaaagaagta gtagatcagg
     3301 taacgatgaa gcgagcattg actcgaatta cttacgagat tatcgagaga aatcatagta
     3361 tccaagatat tgtattagta ggtatcaaaa cacgtggaat ctacattgca tcaagaattg
     3421 ccgaacgcct gaagcagcta gaagatatcg atattcctgt aggagaactg gatatcactt
     3481 tataccgaga tgacaaaaaa gaaaatccag aagaaccgga acttcattct tctgacatcc
     3541 cagtttcctt agaaggaaaa gaagttattt tgattgatga tgttttgtat acaggacgaa
     3601 cgattcgagc agcaatggac gcagtaatgg attttggcag accaagaaaa atttcattag
     3661 cagtcctggt cgatcgtggg catcgagaat tgccaatccg tgcagattat gttgggaaga
     3721 acattccaac atctcgggcg gaagagattt tagtagagat gcaagagctt gatggacaag
     3781 atcgtatcat gattttgaaa gaggaagatt agaggagaga gaaacatgtc ggagaaaaaa
     3841 ggatttcata atgatgacgt ggtgttagat atccacgatc gcccaacccc aggacattgg
     3901 gtaggattaa gtttacaaca cttgttcacg atgttcggag caaccgtgtt agtccccatc
     3961 ttagtaggta tcgatccagg tattgcgcta gtcagttcag gactaggtac attggtgtac
     4021 ttatttacta ctaaaggaaa gatacctgcg tacttaggaa gcagcttcgc tttcattgca
     4081 gcgatgcagc ttttgatgaa aacagatggt tatccagcga ttgcacaagg agcagtcaca
     4141 actggattag tctatttgat tgttgcgctg atcatcaaaa agatcggttc agcatggctg
     4201 gataaaatct tgcctccaat cgtcgtcggt ccagtcatca tggttatcgg gttgggactt
     4261 gcggcaaatg cagcaacaaa tgctatgtat aatcagggca cgtatgattt caagtatatt
     4321 gctgtagcgc tgatcacatt aggattaaca attttcttca acatgtggct aagaggtttt
     4381 ctcggattga ttccgatttt actaggtatc attagcggat acatcgtagc cttgctgttt
     4441 ggaatcgtgg atactaagcc aatcgtagac gctgcctggt ttgctgttcc taactttgag
     4501 attccctttg tacaatatga accaaagcta tatcttggag caatcacaac aatggcaccc
     4561 atcgcttttg tgacgatgac agagcatatc ggccatctta tggtattgaa taaactaact
     4621 aaacgtaatt tctttgaaga accgggtctt cataagacgt tgacaggtga tgggttagca
     4681 caaatcgtgg cagcctttgt cggaggtcct cctgtaacaa gttacggtga aaacattggt
     4741 gttctggcta tcacacgtgt ccatagcgtg tttgtgatcg gtggagctgc agtatttgca
     4801 gtcattttag gattcgtagg aaaattaagc gctttgattt taagtatccc tggaccagtc
     4861 atctcaggaa tcagttttat cttatttgga gtaattgcgg caagtggaat gaagatttta
     4921 atcgagaaca aaatcgattt tgataaaaag aaaaatctgt tgattgcttc agttatctta
     4981 gtagtaggaa ttggcggact cgtctttgaa gtaggcacat ttacattgtc ggcaatggca
     5041 ttagcaactg tacttggcat cattttaaat cttatcttac cagaaacttc acgaagcgaa
     5101 gaaaactaag gaggaaacat tatgatcatc aaatcagaac gtatcagctt aaagcattta
     5161 ttgaccgttg aggcacttac tgatcaagaa gttatgggct tgatccgcag agggcaggaa
     5221 ttcaagcaag gtgctaattg gactccgcaa aagcgccagt actttgctac aaacctcttt
     5281 ttcgaaaata gtactcgcac acataaaagc ttcgatgtag cggagaaaaa actagggcta
     5341 gaagtgattg aatttgaagc aagcacaagc tctgtccaaa aaggggaaac actatacgat
     5401 acagtactga cgatgtcagc attaggcgta gacgtagcgg ttatccgtca tggtgatgag
     5461 aactattatg acgaactgat acaaagtaaa acaatccagt gttcgattat caatggagga
     5521 gacggaagcg gacagcatcc aacccaatgc ttgcttgatt tgatgaccat ttatgaagaa
     5581 tttggtcatt ttgaaggtct gaaagttgcc atcgtaggtg atatcactca ctctcgcgta
     5641 gctaaatcca atatgcagat gttaaaacgt ctaggcgcac aagtattctt ctcaggtcct
     5701 agagaatggt acgacgatga attcgaagtg tacggacatt atatgccatt ggatgaacta
     5761 ctagatcaag ttgatgtcat gatgatgctt cgggtgcaac acgaacgcca tgatggaaaa
     5821 gaaagtttct caaaagaagg ctaccatcag gaacatgggt taacagttga gcgtgcgaag
     5881 aagttgcaaa aacatgcaat cattatgcac ccagcaccag ttaaccgcga tgtagaatta
     5941 gctgattcat tagtggaagg attacagtcg cggatcgttc agcaaatgag caatggagta
     6001 ttcgtcagaa tggcaatctt agaagcagtg ctaaatggaa aagcgtaatt ataggaggat
     6061 gaaaaaatga aaacactcat taaaaacggt cagatcaata cacgaaaaaa tatgacgaca
     6121 ccagcagaaa tttggataga agatgggcga atcaaagcaa tcggtacagg attctcagaa
     6181 gccgaatttg atgaagtgtt tgatgcaaag ggacaattga tcacacctgg tttagtggac
     6241 gtccatgttc atttgagaga gccaggattt acatacaagg agacgatcga agctgggact
     6301 cgctcagctg ctcgtggcgg tttcactact gtatgcgcga tgccgaattt aaatcctgta
     6361 cctgatacgg cagaaaagct gagacaagta tatgacatca ttagaaaaga tgcagttgtg
     6421 aaggtattgc agtatgcgcc aatcacagaa aatctgcgaa gtgagaaatt agttgatcaa
     6481 gaagcactga ttgaagaagg tgcttttgct ttcacaaatg acggtgtagg tgtccaaaca
     6541 gctgggacga tgtatctggc tatgaaagaa gctgcaaaaa acaataaagc cctagtagcc
     6601 catacagaag atgaatcttt attatttggc ggcgttatgc acgctggcaa gaaggcagaa
     6661 gaacttggtc ttcccgggat cttaagcgta accgagtctt ctcaaattgc tagagatctg
     6721 ttgctagctg aagcaactgg tgttcattac catgtttgcc atgtttctac taaagaaagt
     6781 gtccgtgtaa tcagagacgc aaaaaaagca ggaatccacg taacagcaga agtttcaccc
     6841 catcatctta ttttgatcga cgaagatatt cccgaagatt tcggcttttg gaaaatgaac
     6901 ccgccgctaa gaggacggga ggacagagaa gctttgatcg aaggcttgct tgacggtacg
     6961 atcgattgca tcgctacaga ccatgctccc catggattag aagaaaaaag ccaaagtttc
     7021 atgaaatcac catttggtat cgtaggaagc gaaacagcgt ttcaactgat ctatactcat
     7081 tttgtagaaa ctggtcgatt cacgttggaa caagtgatca attggttggc agttaaaccg
     7141 gctgagattt ttggtttgaa tgcaggaacg ctaacggttg gtgcccccgc tgatgtagca
     7201 gtttttgata ttacacaaac ttgcacgatc gataaagaag actttttatc aaaaggggag
     7261 aatacacctt ttatcggctg gaaagtaaaa ggcgaaacac aaatgacttt tgtcaacgga
     7321 aaacttgtgt ggcagaaggg agaataaaag tatggaacgt ttgcttatat tagaagatgg
     7381 aacagttttc gaaggaaagg cttttggtgc agatgctagt gtcttcggag aaatcgtttt
     7441 tacaacaagt atgactggtt atcaagaaac tatcactgat caaagtttta acggtcaaat
     7501 cattacattt acctatccta tggtgggtaa ttatggggta aatagagatg attatgaatc
     7561 gatttctcca acatgcaaag gagtagtagt aaaagaacat gcacgtgtag cttcaaactg
     7621 gcgaaaccaa atgacgctag attcattttt gaaacaaaaa ggaattcctg gaatatcagg
     7681 tatcgatacg cgcgccctta ctcgcaaaat ccgtgaagtc ggaacgatga aagctagtat
     7741 cgttgatgct ggagattcct tcgaacatgc atttgatcag ctgaaagcaa ccgttctagc
     7801 aaccaatcaa gtgcagcaag tctcaactaa caaaccttat ccaagtccag gaacaggtcg
     7861 gaaagtcatt gtcattgact tcggcttgaa acatagtatt ttacgagaat tgagtaaaag
     7921 aaactgtaac ctcacagtac ttccctataa cacagatgcc gagacgattt tagcgctatc
     7981 accagacgga gtcatgctga cgaatggacc tggtgatcca aaagatgtgc cagaagcaat
     8041 cgaaatgatc aaaaaaatcc aaggaaaagt accgattttt ggtatttgtc tcggtcacca
     8101 gttatttgct ttagccaacg gtgcagatac ctataaaatg aaattcggac atcgaggatt
     8161 aaatcacccg gtacgagaag tagcgaccgg aagaattgat tttacttctc aaaatcatgg
     8221 ctatgcagtc gatgaagcca cgattgatcc agaaaaactc atcgtaactc atgtagaagt
     8281 aaatgacgga acagtagaag gagtcaaaca cagagattat cccgctttca gtgtgcagtt
     8341 tcatccagat gctgcccctg gtccgcatga tgcactacat ttatttgatg aatttatgga
     8401 aatgatggac gcaggaaagg agcagtaaac agatgccgaa acgtacagat atcaagaaaa
     8461 ttatggtgat tggatctggt ccgatcatca ttggtcaagc cgcagaattt gactacgcag
     8521 gaacacaagc ctgccttgcc ttaaaagaag aaggatatga agtggtttta gtaaattcta
     8581 atcctgcgac gatcatgacg gataaagaag tggcagatca agtatatatc gaaccgatca
     8641 cacttgaatt tgtatcaagg attcttcgaa aagagcgtcc agatgctctc cttccaactt
     8701 taggcggaca gacaggcctc aatatggcaa tggagctggc agcatcaggt attttagaag
     8761 aattgaacat agaacttcta ggaacaaagc tttctgcgat cgatcaagca gaagatcgtg
     8821 atctattcaa acagttgatg aaagaattga atcagccaat ccccgagtct gaaatcgtca
     8881 atacagtaga agaagctgta gattttgcaa ataaaatcgg ctatcctgtc atcgttcgtc
     8941 cagcctttac tttaggcgga actggtggag gtatgtgtga aaatgaagaa gaattacgta
     9001 tcgtatcaga aaatggatta agtctttccc ccgctaccca atgtttgatt gaacgaagta
     9061 ttgcaggttt taaagaaatt gaatatgaag tgatgagaga ttcggctgat aatgcaatcg
     9121 ttgtctgcaa tatggaaaat tttgacccgg tgggtatcca tacaggtgat tctatcgtat
     9181 ttgcccctag tcaaacattg tctgaccatg aatatcaaat gcttcgtgat gcttctcttt
     9241 cgattattcg agcacttaag atcgagggag gctgtaacgt ccaactcgct cttgatccac
     9301 atagttttaa ttattatgtg attgaagtca atccgcgtgt ttctcgttca tctgcgttag
     9361 caagtaaggc gactggctat ccgatcgcta agctagctgc taaaatagcg cttggattaa
     9421 cattagacga aatgaaaaat cctgttacag gaacgacttt tgcagaattt gaacctgcat
     9481 tagattatgt tgtagctaaa attcctcgct ggccgtttga taagtttgaa aacggagaac
     9541 ggcttttagg cacgcagatg aaagcaactg gtgaagtgat ggctatcggt cggaatatcg
     9601 aagagtcttt attgaaagct gtgcgttctt tagaaatcgg tacataccat atcgaacttc
     9661 ctgagttggc agacatcacg gacgatgacc taatccaaaa agtggttcga gcacaagatg
     9721 atcgcctgtt ttatgtagct gaagctatta gaagagggta caccatagaa gaattagcgg
     9781 atttgacaaa aatcgattta ttctttttag ataaactgtt gcatatcgta gaaatcgaac
     9841 agcagttgaa acagcagccg ttggatgaca agatattgaa aacagcgaaa caaaacggtt
     9901 ttacagacaa aaaagtggcg gagctgtggg aaataaacga agaagccgtt cgccagatga
     9961 gaaaagaaaa tgatttgatt cctgtatata agatggttga cacttgtgca gcggaattcg
    10021 aatcaaaaac accttatttt tatagttcct atgaatatga aaatgaaagt tcacgatcag
    10081 aaaagccctc aattctagta ctgggatctg gtcctatccg tatcggacaa ggggttgaat
    10141 ttgactatgc gactgtgcat tctgtcaaag caatccagca ggcaggatac gaagcaatca
    10201 tcatgaacag caatcctgaa acagtttcga ctgacttttc tatttcggat aaattatatt
    10261 ttgagccatt gaccttcgaa gatgtgatga atgtgatcga gctagaacag ccgataggag
    10321 taatcgttca atttggtggt cagacagcaa tcaacttagc cgaacctttg actgctgctg
    10381 gtgtaaaaat cttagggaca acgatcgaag acttggatcg tgcggaaaat cgtgatttat
    10441 tcgagcaagc gctgaaagag ttggatattc ctcaaccttt aggggatact gctacaagta
    10501 aagaagaagc cgtggttatc gcttcgagaa ttggttatcc agttcttgtt cgtccaagct
    10561 atgtattagg cggacgtgcg atggaaatcg ttgaaaacca gcgagattta gaagattaca
    10621 tggaacatgc tgtgaaagca tctccagaac atccagtact agtggaccgt tacttgcttg
    10681 gtggtgaatg cgaagtggat gcgatttgtg acggtgaaac agtactcatc ccagggatca
    10741 tggagcatat tgagcgtgct ggtgtccatt caggggattc aatggctgtg tatccacctc
    10801 aaacattatc tgaatcgatc aagaaaacga tcgaagatta cacgatccgc ttagcattag
    10861 gtttgaattg tgtcgggatg atgaatatcc agtttgtcat ccatgaaaat gaggtttatg
    10921 tgatcgaagt caatcctcga gcaagtcgga ctgttccatt cttaagtaag gtgacaggaa
    10981 ttccaatggc acaaattgcg acgaaagcta ttttgggaga gaaactaaag acacttggtt
    11041 atgaaaacgg actttatcca gaaacaaaag aagttcatgt taaagcacct gtcttttctt
    11101 ttacgaaatt gcagaaagtt gatacttact tagggcctga aatgaaatcg actggagaag
    11161 taatgggatc tgacagacaa ttagacaaag ctttgtacaa ggcattcgaa gcttctggat
    11221 tacacttgcc tgaatatgga gccgtattat ttacgattgc ggacgaaaca aaagaagaag
    11281 cactagaatt agctaaacgt tttacagaaa ttggctatag tctgatagga acgaaacata
    11341 cagctgctta tttcgaaaaa aatggtctgg tcgttgaaac agtagcgaaa atttctgagg
    11401 aagcagcaga aaaaaatgta gtcgacttaa taagagatgg taaaacacaa gtggttgtca
    11461 atacgattga taaagatcgg ggaaatgctt ctaaagacgg tttcatcatc cgaagagaag
    11521 cagttgaaca tggtattcca ttgtttactt ctttagacac agcagatgcg atcgttcgag
    11581 tgatggaatc ccgtgctttt tcaactaacc ctatttaacg gcgagcacat gttaagacaa
    11641 aaagacgaaa aaccgttgca ggaggggaca gatgaaacaa gaaatgatgg aaatcgttca
    11701 gcaaaaacag ctggcaccaa agatttttga aatgacattg aaaggtgatc tagtaaaaga
    11761 aatgcagcaa ccaggacagt tcttgcatat tctcgtgccg cgtgcagact tgctattacg
    11821 ccgaccgatt agcttgaatc atatcgacca cgaaacaaat acttgccgta tcatttatcg
    11881 ggtggaaggt gaggggacta gagtcttctc tactctttct gccggagacc atttagatgt
    11941 catgggtcct ttaggcaatg gttttgattt gtccaatctt catacaggag acgaagtatt
    12001 tataatcggt ggaggaatcg gtgtccctcc tctatatgag ttatcaaaac agttagtcga
    12061 aaaaggaatt aagccaacgc attttttagg ttttgccaca catgaagtcg tttattatga
    12121 aaaagaattt aatcgtctag gagaaacaag aatcgctact gacgatggaa catacggtat
    12181 gcacggaaat gtcggtaatt tattgttagg aacgaagacg gaacctacag ctgtttttgc
    12241 atgtggttca aacgggttat tgaagacagt ggaacagctg ttttcttcgc atcaaaatgt
    12301 ccagttatca ttagaatccc gcatggcatg tgggataggg gcttgctatg cttgcgtttg
    12361 tcatacgccg gaagatgaaa caggaacaaa aagtgtgaaa gtttgtgacg aaggaccgat
    12421 ttttaaaata ggagaggtaa ttatatgaat gagaatcctt tagcagtcaa gctccccggt
    12481 ctagaattaa aaaatcctat catgccggca agcggctgtt tcggttttgg caaagaatac
    12541 ggaaaatact atgatttgaa tcagcttgga tcgatcatgg tcaaggcaac gactccaaat
    12601 gcccgctttg gcaatcccac accaagagtg gcggaaacac catctggcat gttgaatgca
    12661 attggtttgc aaaatcctgg tctagacgtt gttatgcata aacttcttcc agaattagag
    12721 gagtacaatg agttacccat catcgcaaat gttgctggtg cttgtgaaga agactatgta
    12781 gaagtttgta gcaaaattgg cgaagcgcca aatgtgaaag caatcgagct gaatatttct
    12841 tgcccaaatg tcaaacatgg cggaattgct tttggaacag atcctgatgt tgcttttcaa
    12901 ttaacacaag cagtaaagaa agtttcttct gttcctgtct atgtcaaatt gtccccaaat
    12961 gtaacagaca tcgttccaat cgctcaggca atcgaagctg gaggggcaga tgggtttacg
    13021 atgatcaata ctcttcttgg tatgcggatt gaccttaaga caagaaaacc gattttagcc
    13081 aatcaaacag gtggactctc cggaccagca atcaagcctg tcgctattcg tttgattcac
    13141 caagtagcaa gtatatccag ccttccaatc attgggatgg gcggcgtgca gacagtggat
    13201 gatgttcttg agatgtttat ggctggagca agtgctgtag ctatagggac cgcgaatttc
    13261 accgatccgt atatctgtcc taaactaatt aaagagctcc ctgtgcgaat gagcgaacta
    13321 ggcatcgaat cgctggaaag attaataaaa gaagtgagag aggaacgtga acgatgaatc
    13381 aactagacca acgaccaatt attgcattag atttttcaac taggcaagag gtagaagact
    13441 ttcttcgatt tttcccgaaa gaagaaaaat tgtttgtgaa aatcggaatg gaattgttct
    13501 accaagaagg acctgaaatc gtacgttatc taaaagaggc tggtcataat gtatttttag
    13561 atctgaaact tcacgatatt ccgaatacgg tcgaaaaagc aatgcgggga ttggcaaagc
    13621 ttggtgttga tctcacctgt gttcatgccg ctggcggtat tcgtatgatg gaggcggcaa
    13681 tgcgaggatt agaagagggg actccagaag gtggaaaacg tccgctactt ctagctatca
    13741 ctcagcttac ttctacaagt gaagaagaaa tgcatgccga tcaactaatc gaagtttctt
    13801 tagaaaaaag tgtgatccat tatgctagct gtgccaaaaa agcaggatta gatggtgtgg
    13861 tttcttctgc ttgggaagta gaagcgatca aagaaatggc aggagacgaa tttgtttgct
    13921 taactccggg tataagacca gaaggaacag tcgcaggaga tcagacaaga gtagtcacac
    13981 cttctcaggc aaaagagatc ggctcaactt ttatcgttat aggtcgacca atcactcagt
    14041 caactaatcc ttataaagca tatcaaacga ttcaaacaga atggagctaa cctaaaatga
    14101 atgttgaaca aagtatcgca aaagacttat tagaaattga agcagtattt ttaaatccaa
    14161 gtaatccttt tacatgggca agtggaatca aaagcccaat ttactgtgat aaccgaatca
    14221 cgatgagcta tccaaaagtc cgaaaagaga tcgcaaaagg attagcaagc aaaatcaaag
    14281 aagcttaccc tgaagtacaa gtgattgctg gaactgcaac agcaggaatc cctcatgccg
    14341 catgggtagc ggaaatactg gatttgccaa tggtctatat tcgaagtaag gcgaaagatc
    14401 atgggaaagg taaccaaatc gaaggacgta tatttgaagg tcagaagatg gttgtgatcg
    14461 aggatttgat ttctacaggc ggaagtgtct tggaagcagc tgaagcagca aaaagagaag
    14521 gtgcggatat ccttggtgtt gcggctatct ttacgtatga gttgccaaaa ggaaaagcaa
    14581 atttcgaaaa agccgaaatc cctcttatga cgttgaccaa ttattctgta ttgattgaag
    14641 cagctttaga agatcgttat atcgatgagc aagaattaac tctgctaaaa gaatggaaaa
    14701 aagatcctga aaattggcag atttaagcgc gcttgatgaa gatagagaaa tccgatttgt
    14761 gtctccagaa tcatcaattg gaatagcaaa aatccaaaaa ctgattgcga gaagcatctt
    14821 ttcatcaggt atgaaaagaa acttctatcg tgatcagttt ttgatttttt taaaattcat
    14881 ttttctaaga ctggttagca gaaagaagag aataatctct tc
//
LOCUS       DAGCYL010000061        14485 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.062, whole genome shotgun sequence.
ACCESSION   DAGCYL010000061 DAGCYL010000000
VERSION     DAGCYL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.062"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(199..1647)
                     /gene="cls"
                     /locus_tag="MB216_001389"
     CDS             complement(199..1647)
                     /gene="cls"
                     /locus_tag="MB216_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBT4526231.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1799..2692)
                     /locus_tag="MB216_001390"
     CDS             complement(1799..2692)
                     /locus_tag="MB216_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4526232.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2676..3362)
                     /gene="ftsE"
                     /locus_tag="MB216_001391"
     CDS             complement(2676..3362)
                     /gene="ftsE"
                     /locus_tag="MB216_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBT4526233.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3467..4568)
                     /gene="prfB"
                     /locus_tag="MB216_001392"
     CDS             complement(join(3467..4495,4497..4568))
                     /gene="prfB"
                     /locus_tag="MB216_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBT4526234.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4694..7228)
                     /gene="secA"
                     /locus_tag="MB216_001393"
     CDS             complement(4694..7228)
                     /gene="secA"
                     /locus_tag="MB216_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBT4526235.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            7554..8855
                     /locus_tag="MB216_001394"
     CDS             7554..8855
                     /locus_tag="MB216_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4526236.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(8951..9502)
                     /gene="raiA"
                     /locus_tag="MB216_001395"
     CDS             complement(8951..9502)
                     /gene="raiA"
                     /locus_tag="MB216_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBT4526237.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(9630..10349)
                     /locus_tag="MB216_001396"
     CDS             complement(9630..10349)
                     /locus_tag="MB216_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBT4526238.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(10346..11659)
                     /locus_tag="MB216_001397"
     CDS             complement(10346..11659)
                     /locus_tag="MB216_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBT4526239.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(11740..12060)
                     /locus_tag="MB216_001398"
     CDS             complement(11740..12060)
                     /locus_tag="MB216_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4526240.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(12168..12455)
                     /locus_tag="MB216_001399"
     CDS             complement(12168..12455)
                     /locus_tag="MB216_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526241.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            12582..13226
                     /locus_tag="MB216_001400"
     CDS             12582..13226
                     /locus_tag="MB216_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBT4526242.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(13285..13992)
                     /locus_tag="MB216_001401"
     CDS             complement(13285..13992)
                     /locus_tag="MB216_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4526243.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(14212..14382)
                     /locus_tag="MB216_001402"
     CDS             complement(14212..14382)
                     /locus_tag="MB216_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526244.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         4085 a         3118 c         2525 g         4757 t
ORIGIN      
        1 actggctttg aaatgtgtct cttttaatag catatattag cttatttctt gctttcccca
       61 aaaaacaaga gataccccaa agttaatttc gctcatgcga gacgctttct ccatttctga
      121 cgtactctca gaaatggccc cgttagaaaa ggacacgaga tctccatctg ttgatggttc
      181 tcatgtcctt tttatgtttt aaaggagcgg cgaaatcaat cttgctaatc cttctttcat
      241 cttgatgatt attcctcggt tttcatacat ttcttttgtg agatgcgtag aaattttcga
      301 gtcttcaaaa aaagcttttc ttacacgtga agccattcgt tcatcataaa ccagtgtatt
      361 gacctcaaag tccaagcgaa atgaacgaac atcgatattt gctgagccaa ctgaaacaat
      421 gccaccatcg ataatcatcg tctttgcatg aataaatcca ttctcatatg tttcgatcct
      481 tgctccatac tcgattagct ctgcagcgaa agaatacgtt gcccagtaaa cgagaagatg
      541 atctggtttg ttaggtattt ggatatgaac ttttacgcct gaaagaagag ccaacttcag
      601 tgcttcatga atcgatccat ccggtatgta ataaggcgtt tggatcaata tctcccgttt
      661 ggctaacgaa atcattttca aataagtcat tttgatttgt tcatgttcgg tatctggtcc
      721 gcttgtcacg atttgaacag ctatatcacc agtagagtga atggacggaa aaaatttggg
      781 agaatagcct acttcttttg tatgctgaga attccagtcc atcaaaaaac ggttttgcaa
      841 ggtatacacc gcttctccgt aaatacgcaa atgattgtct cgccaataac caaatttctt
      901 taccaatcct agatattcat ttccaacatt gaacccacct gtatatccga ttttcccatc
      961 aataactacg attttacgat gattgcgata attgatccga ggattaatat acggaacaaa
     1021 tagaggaaag aaaaatgcga cttctccgcc tgcttttttc aaattctgaa aaaagctgga
     1081 agaaacttgt gtggaacccc atgcatcaag aaggactctg acttttaccc ctcgtcttgc
     1141 cgcctctgtc aatgcatctc gaacttcttc acctaatgca tcactgcgat aaatataata
     1201 ctggaagtgg atatggtctt ctgcctgctt gatatcttca atcagcgcat cgaatttttc
     1261 acgaccatct gtaaataaag tgatttcatt tttagtcgta tagagcgtac tttcaaatac
     1321 cgtgagcata tagatcaatt gtttcacatc tacttgtcca gtaggcggat gaggataaag
     1381 ccctcgcatc aatgcctgtt tgtcttcttc aatttccagg ttcttaccga ttttcccttg
     1441 gatcttcaaa tcaaagattt tctctttgga aatccctctt cctaaaaaaa tatataaaat
     1501 aaatcctaca actggaatga acatcaaaac gagcacccat gcccatgttt gagcagtttc
     1561 ctttctttct cgaaaaacga tgataaagga aagcaaaata ttcataatca aaaggatcgt
     1621 aaaaaaattg tcaacgataa ttttcacggc ttcacctctt cttctaccaa cttaagaata
     1681 cccaagttgt tttttaaagt aaagggaaaa aagccgcttc cttctattct cctagccaat
     1741 aaaaaaagaa agctggaacg aaactgtcca actttctcat tttatttata ttgtggtatt
     1801 agaatttcaa gaaacgtctc atagaaatga tcgagccaag tgaaccaatg atcacaccaa
     1861 tgccgaccat taaaagattg attttccaaa cgatattttg tggatgaatc aatgaataat
     1921 gggaacgtaa taatgatgga ttgatcaatt ggaaaaacca tgcataacca aatgtgatga
     1981 tcaaaatcgg aagaatcgca ccgatcagac cgatccagcc accttcaagg aaaaacggcc
     2041 agcgaatata tccatttttt gcaccgacta aacgcatgat ttgaatttca cgctgtctag
     2101 acaaaatcgt gatccgaatc gtgttcgaaa tgagaaacat cgctacgaac agcagcaatg
     2161 ccgctgcaac taatccccat gtccgaactg caccagcgat ttggaaaatc cgatctgagg
     2221 aagctccacc gtaatcagaa cgagaaacat tcttcaaatc tgctgcttct tctgctactt
     2281 gttttgtata agaaggatca gtagcactta ctacatacac atcatataac gggttattgt
     2341 caccttcaaa caatttccag gaatcaccca tttcgttttg gatttttttc aattcctgtt
     2401 ctttgcttga aaattcaact tttttaacat gatctaagtc attcaattct ttttctagct
     2461 ttttcatctc ttcgtctttt gtaccgatat ctacgaatac agagacatca acgtttcctt
     2521 ctatatcttg agctaattta gttgcgttca tgataactgc catgaataca ccgactaaag
     2581 tcaatgtgat cgttaccgca ctgactgaag caactgtcat ccagccgtta cgtttcaaac
     2641 tcttgatact ttctaataaa tggcggaaaa aggttctaat catcgtatcc atattcccct
     2701 tccgcttgat ctcgaataat gcggccgttt tcgatagcaa ttactcggtg ccgaatcgta
     2761 tttacgatcg tactgttatg tgtagccatc acgacagtag ttccttgggc attgatccga
     2821 tctaataatt tcatgatttc ccatgagttt tctggatcca gatttcctgt cggttcatcc
     2881 gcgataagta ctttaggcgt attcactatt gcccgagcaa tagagacacg ttgctgttcc
     2941 ccaccggata attcgctagg aaagactcga actttgtgtt tcaaaccaac aaggtccagc
     3001 acttccatta ctcgtttttt gatatcccga ggttttctgc cgataacttg catagcgtac
     3061 gcaacatttt catatactgt ttttcttggt aataatttat agtcttggaa caccacgccg
     3121 atttctctgc gtagataagg aacttcgcgg tttttgattg ctattagatc atgacctgcg
     3181 acgttcaaag ttccttttga agctttttct tctcgataca tcaatttgat gaatgtagat
     3241 ttaccggctc cagacggacc aaccacatag acaaactctc cttgttcgat gacaattgag
     3301 agattgcgga tagcggtcgt accatttgag tacttcttca ttacatcctt catttcaatc
     3361 atggcgttct ctccatatct ttttcagtct ttagctatta tagcatggca atgtttcaac
     3421 atgcttgcaa aatcttacaa ttttatttca atttaacaat gggttcttat tgtagatgca
     3481 tttttaaata gccgtcaata aataaatcaa ggtcaccgtc cataactgct tggacatttc
     3541 ctgtctctac gtttgtccga tggtctttta ccatagaata tggatggaaa acataagaac
     3601 ggatctgtga accccagccg atttcttttt gttctcctcg aagtgcagct gcttcttgtt
     3661 ctttcttttc cacttctaat tgataaagct tagcttttaa catccccatc gcttgctcac
     3721 ggtttttcaa ttgtgaacgt tgtgcctgac tagctactac tgttcctgta ggcaagtgag
     3781 tgatccgtac agcagattct gttttattga tgtgctgacc acctgcacca cttgctcggt
     3841 acgtatcgat tttcaaatca tctggattga tttctatatc gatcgtttca tcaagctctg
     3901 gcatcacatc taccgaacaa aaagaagtat gacgtcgttt cgctgagtca aaaggcgaga
     3961 tacgtacaag gcgatgaaca cctttttctg atttcagata accaaatgcg ttgtgtcctt
     4021 tgatcagtag ggtcacactc ttgattcctg cttcatcacc tgcttgataa tctagtgtct
     4081 ctacttgata cccatgctgc tctgcccagc gtgtatacat acgtaaaagc atactgcccc
     4141 aatcttggga ctccgttccg ccagcccctg gatgcaattc aacgatggcg ttgtttcgat
     4201 cataaggacc atctaacaac atagataatt catatgtctt cattttttca tctagcgcat
     4261 tgatacgtgt ttctgcttct tcttgcatgg ctgcatcagg ttcttcctgc aacatctcca
     4321 ccatcaattc gatttcttcc agttcttcag caagagaatg gaattgatca taagtttctt
     4381 tatttgcatt gttttcactg atcacttttt gagcagcttc gctatcatcc caaaaaccag
     4441 gttcagccat ttttgcttct gcttccgcaa tgtcttcttc cagctggtct aagtcaaaga
     4501 gacctcctga agcttgtgac cttttcctgc atttctgtca ggagatttcg aatttcggat
     4561 atttccatgt tttttccttc tttcactata cttttttcat tatatctagt ctaacagaat
     4621 cagcaaataa cagaaagaag gtgagacgaa ttcttaaaaa aagaatttgt ctcaccaatc
     4681 ctgctgtcag aaattagttt gcattttttc catgacagtt cttatatttt ttcccgcttc
     4741 cacaaggaca aggatcattt cttccgacct tgttcacatg gattggctgt tttttcgcgc
     4801 tagtattact attttctttg gcatctccgt cttctgagtg agaagctgtt ccttgagaaa
     4861 cctgttcacg ctgtacgttt tgacggatct cagctttcat aaagattcga gtgacttcat
     4921 actcgatggc gcctaccata tcttcaaaca tcttatagcc ttctgtttga tattcgacta
     4981 atggattgtt ctgtccataa gcacgtaagc cgattgactg acgtaattga tccattgcat
     5041 caatatggtc tgtccattta gagtccacaa cacgcaagat cacgactttt tcaaattcca
     5101 acagttgctc tggaccattt agttgagccg ctttggtatt aaatacttct tgtgctcgtt
     5161 cgtttagata ctctttgatc tcctgagctg tttttccttc gatatctgca agagtaatac
     5221 tatcttcatg gacaagagta ctgccggcaa aatcgatgat actttccaaa ttccaatttt
     5281 ctttctctaa ttgtgtatgc gcatctacta cacgactgat tgtccgtttg accatgttca
     5341 ttagtggctt ggataagctt ttttcttcca tgattacttc ttgtcgctgt ttatagatta
     5401 cttcacgttg ttcacgcatc acatcatcgt attgtaagac gtttttacgt gtgtcatagt
     5461 tatttccttc tacacgtttt tgtgcagatt ctacttgttt cgtcagcatc ttgctttgga
     5521 taacagcatc ttcctcaccg atcttcatcc gatctaagaa agatttgatt cgttctgaac
     5581 cgaatcgttt catcaaatca tcttccaatg acaagtagaa ctgtgacata ccagggtctc
     5641 cttgacgtcc tgcacgtcct cgaagctggt tatcgatacg acgagattca tgtcgttctg
     5701 tcccgataac tgctagacca ccaagttctc ttaccccaag tcctaacttg atatctgttc
     5761 cccgaccagc catgttggta gcaatcgtca cagcaccttt ttgaccagca ttcatgatga
     5821 tctctgcttc tttaaagtga tttttcgcat tcagcacttc atgaggaata cgttcttgat
     5881 tcagcatatc tgataataac tcagatgttt caaccgcaac agttccgact aacacaggtt
     5941 gacctttgcg gtagcgatct ttgatatctt gcacgacagc gtggaacttg ctttgtaatg
     6001 ttgggtaaag caaatctgga cgatcatcac ggataaccgg acgatttgtc ggaatctggt
     6061 atacttggat gttataaatt tctctgaatt cttcttcttc tgttttagct gtaccagtca
     6121 ttcccgcaag tttcttgtac atacggaaat agttctggaa agtgatcgtc gccatggttt
     6181 tcgtttcgtc ttcgatctct acgccttctt tagcttcgat tgcttggtga agtccatctg
     6241 aataacggcg tccatccatg atccgaccag tgaactggtc aacaatcagt acttttccgt
     6301 cttggacaac ataatcgata tctaaaagca tgatatagtt ggctctcaat gcttgatcca
     6361 agtgatgggt caatgcagta ttttcaatgt cgtataagtt atccaaaccg aatgtttctt
     6421 ctgctttttc gatccctgct tcggttaaac tgattgtttt tgattgtaca tcgattttat
     6481 aatcttcttc ttctttcaaa cgtttcacga agttgtctgc tcttgtatat aaagctgtgg
     6541 atttttctgc ttgccctgaa atgatcagcg gcgttctcgc ttcatcaatc aagattgagt
     6601 caacttcatc gacaatggca taattcagcg gacgttgtac catttgatgg cggtaaacga
     6661 ccatattgtc tctcaagtag tcaaatccta attcattatt cgtactataa gtgatatcac
     6721 aattataagc ctcgcgtttt tcatccgaag attttgagtt gatattcaac cccactgtta
     6781 atcctaagaa attataaagt tcgcccattt ccgtcgagtc acgggttgct aaatattcat
     6841 tgacggtaac tacgtgtacc ccttcgccag tcagagcatt tagatatact ggcatcgttg
     6901 cggtcaaggt tttaccttca ccagtacgca tttcagggat atttccatcg tgaagcacga
     6961 ttccacccat caattgtacg tggtgtggat ataaccccaa tacacgtttt gctgcttctc
     7021 ggacgactgc aaaagcttcc ggaagtaatt gatctaatgt ttctcctttt tggtatctgc
     7081 ccttgaattc agcagttttt gcctgtaatt gttcatcagt taatgctgcc atttggtcag
     7141 cgtatgcttc aacttttttt gcaatgctat ctaaacgttt tagttctttc ttatcgtttt
     7201 cgataagtga acgaatcaaa ttggccatgc gttgtgtaat ctccttttcc ttctgtgtat
     7261 tatccagact atgtagactt tacttttcat tttatcatta gttatagata attgaaatat
     7321 cttaagcgta ttaaagtaag aacttcataa tttattcata tttcatctaa aagaggagga
     7381 aactcaaaaa aacttgcggc agctgtcagt agctgctgca agttttcggg ctctataatt
     7441 tctaggactg atagaaaatc attctatcgg tcccttttga gcatgaaaaa acaaaacata
     7501 cagaagctcc gttagaattg aatcaccaca acacaatcaa aggagcgttc tgtatgcctg
     7561 atcttcatca tatcaaacta ctacttggaa tcaaagataa aaacattttc atcacaaatg
     7621 tggaatcgaa gagcattaag aatatcaaga gtcttgttgt ttctgcgacg ttaagtaaag
     7681 aaattcgtcg ctgtcctttg tgtaaacaga tgaatcatga aggcatgatc gttaagaacg
     7741 gaaagaaaaa atcgctcatt caacttaata aatgtgcgaa ccaactgact tatcttgctt
     7801 tagcgaaaca acgctaccac tgtcgtggct gccacaccta ttttacggcg aatacctaca
     7861 ttgtcgatag aaactgcttt atcgctaaac aagtgcgcta taaaatttta gaagagctaa
     7921 ctgaaaaaca agcaatgacg acaattgcta agcattgcgg cgtttcttgg tcgaccgttt
     7981 ctcggacact cgcttctctg atccccatga caaaagtaaa aagaaattgg ttaccgcgtt
     8041 gtttattaat ggatgaattt cgttcactga aaaatcaagt cggtccctac tccttttctt
     8101 gtatggatgg cgatactgga aaattactgg atattcttcc ttcacggaag aaaaaggctc
     8161 tcgtttctta ttttatgcaa tttgaacgac gcgcacgatt gaaggtaaag gtcatcgtga
     8221 cggacatgaa tgtttcttat gcctctctga taaaggaatg ttttccaaag gcaaaactcg
     8281 ttgtggatcg ttttcacatt gtcaaacatc tcattcgtaa atttgaggat attcgcgtac
     8341 gaatcatgaa aagctttgat cgaaacgatc caacacaagc caaatattat cgacaattaa
     8401 aggcgttgtc tcggcttctg atcaaacgac aggacaagct tgtttatgac aaatggacca
     8461 aatggcgcaa ttttggttgg gcgtatctca ccgaaagtga agtggtggat cgtctgcttt
     8521 cgatctctga cgaattacga atcgcctatt tttactatca agaaattctt caagcgtttc
     8581 acgacaaaga ggcggacact ttctttaaac ttgtaagaac gatgcccaat tccgttccaa
     8641 aagaactgca ttctataaaa aaagctttta taaactatga atccggcatt cgtttagccc
     8701 tcgaattgcc ttattcaaat gccaaaatag aaaatctgca tacccacatc aaagcattga
     8761 aacgagtcgc ttatggcttt cgaagctttc ggaaaatgaa gaccagaatt ttcttactaa
     8821 ataatctgat tacctatgaa tcaaaaaaca tctaaagtag aaaaggaatt ctaccttaga
     8881 tgttcttatc tcactctatc agtcctattt gacaaagagc cagttttcgt tgttatttaa
     8941 gtaaggctga tcagtctgtt tcaatcagtc cgtattttcc atctttacgg cggtaaacga
     9001 tacttgtacc atttgtttct gcatcttcaa aaataaagaa gttatgtccc agcatgttca
     9061 tttgtaagac tgcttcttca ctatccattg gtttcaatga aaggcgtttc gtacgtacga
     9121 tatctaattc agaatcattt tcgttttcgt cctcttcatt gaataaagca gcagcatctg
     9181 cagtattcat gcccgtttca cgggatttac gattaatttt tgttttgaat ttgcggattt
     9241 ggcgttccaa tttatcaaca accaaatcga tacttgcgta taaatctgga gaagtttctt
     9301 cggcacgtaa tacaagataa ggtaatggga tcgttacttc cacttttgct gttttttctg
     9361 tataaacttt taagttcaca tgcgccgttg catcgggtgc gtcactgaaa tagcgttcta
     9421 gtttgcctac ttttttctca acgtagtcgc gaatcgcttc agttacttcg atattttcgc
     9481 cgcgtacatt atatctaaac ataaaaatta cccctttcgt ctaccacaat caagaaggag
     9541 aaggaccact ctttccctct tcttaattct attatatcga actctaggtt atttgcaaat
     9601 taaatcgcta tcaaatttcc acatctaatt catctcgcca aagaaatggt tcggatgatt
     9661 ttcggctgtg cgacctgcaa acaatctgct gcgtggaaaa gagtctgtcc tgttgtatag
     9721 acatcatcaa taatcaatat ttttttattt tgtatcttag aaaaggtgac tgcaagttca
     9781 aaaggttgag gcatctccaa tctttccttt cgcgtgtttt ttgcttgagc cagcagatgc
     9841 ttctctcttt tgagtagcat ctgaaaagga attcttgctg ctttcaacat ctcgcttact
     9901 tgattgaatc ctctttctgt tcgccttttt tcagataaag gtattggaca atagatgtat
     9961 gtcgggtatt ctttttttat ctgcgtccat ttatatgcaa aggtgcctgc tagtcggata
    10021 tctcccataa acttgtattg attgagccag gccttcatat tttcatcgta cacaaaaaag
    10081 gcacggtgct gaaaatcata ggtcggatat ttcttcttcc attcttgaca gtcactgcag
    10141 taaacgggaa tagactttac tttctttttc gtactctcta gattgaaatg gattggtttt
    10201 tgacagccag aacagcctct tgtttccaat aaatgaaatt gtgcgcgaca aactgaacaa
    10261 gtctgaggta atctgtattt cgaaacacca ataatctcgc tgaatgttaa attttggaag
    10321 atatctttac tgcagcaact gcacctcaca gcatcttctt cccttcttca ttcatttctt
    10381 tgatttcctg acaagctttt cgaatagctg cagttttttc ttcatagaaa aagtatactt
    10441 gactgttcgt ataaggccct ttacgatcgg ctcttcctgc gatttgtacc aaagaagatt
    10501 tagtaaaaac cggatgatcc gctcctaaaa cgataacgga tattctttca aatgtgactc
    10561 cacgttcaag gatcatcgtg gttaacagta tatcatattt tgcttctcgc attttctgga
    10621 ctttttccgc ccgctgattg tctcttgaag aaacatctgt gatctcatat ctcggaagag
    10681 cctcttgaag tgtggctttc aaacgatgca tcatcgtgat acttggacaa aataaaagga
    10741 tatggttttc tttaattaat tgatgaatga tccgcaccaa agattttatc ttcttcttgt
    10801 tcttgcattt ttgtcgccaa ttattcatga aaaccaagac gggttcagga agcaaacgcc
    10861 gatgaaagcg taaagcaagt ttgtggatgt caaacgtctg cttgatttcc tttagtaact
    10921 ttttatcagg tgtggcactt aagtagatga atcgtccgtc tggtttaatc gctgtttgca
    10981 cagcatgagc taatcctgca tccccgttat aaggaaacgc atcgatctca tcgatcacta
    11041 ttaaatcaaa ggaccgataa aaacggtata gctgatgaat cgtacagata acaaaagaag
    11101 taaaggtata agccgttgct gtattcccat ggagcaacgt gattttttct tctggaaaaa
    11161 cataacgata gcgtaaaaac aattcattac aaacatcgac tcgcggtgta caaatcgcta
    11221 ctcttcctcc agcttttaaa gtttccacta aaataggata aagcatctct gttttccctg
    11281 cacctgttac agcccaaata agatgatgtc ttttttctga atggcaaagt attctctttg
    11341 agatttccgt ttgtttttca gtaagctgtc ctgtccaaaa aaagacgact tctcgaggtt
    11401 ttgcttcagg ctgatcaaac aaatataaat cttgttgagt gtcgcatcgc cctaaatata
    11461 gacaatgtgt acaaaaataa ccgtaatctg caagctgtcc atcaatgacg gacgtcctca
    11521 ttccacagcg atgacaaaga atccagttgc ccaaacgttc cactgctgcg atcttctcat
    11581 aatttggtaa atttacctcc tcacagtcta ttgcttcaat atctctcgtc agaagccttc
    11641 taccccaaaa atgattcata ttcttcgccc taaaaaaata tacgaaaaaa caagcgatct
    11701 attttttgga tcgcttgttc gatagttata ctgttctatt tattcaccta tgagcgtgat
    11761 tgcttgatct aacacttttt cgccattcat cataccataa tcagccatat tgatgacatc
    11821 taaaggaatg ccttttggtt ccagacgttt ctcaaaatca gctttcataa agcgaacttg
    11881 tgggcctaat aacaacacat ccacgtcttt atttgcaagg ttgttatctg cgtcagaagc
    11941 ggacacagcg aaaatatccg agtcgattcc ttttgcttca gcagcttttt gcatttttgt
    12001 tactagtaaa cttgtgctca taccagccga acatacaagc atgattgttt ttttagccat
    12061 acttttctct cctttttaaa acaaactatg caaacgtttt cttcgcgtct gttactctat
    12121 tatataaaaa gaaagaaatt tgtcaacgtt ttctaaacgc ggtttattta atcaaatagc
    12181 caactagatt tcactaattt attgaacacg acatattctt tgtccaaatt gatgacaact
    12241 acttcatagc ctgcatttat ccagccatag gcaccttttt cagggtgttt ataatcattt
    12301 gcccaccaat ctgtactttc ccttgctgtt ttgggaagtc cgttactggg aaacagcagt
    12361 tcgtcaaatt gggtaaaggt caacgttact tgagagccgc cattgttctt caaataatgc
    12421 gtaattgcat cgtacttttt cattcgttcc tgcatcccca gatccacctg cctttgtctt
    12481 tattatcgca tactacagca attgcgcaac cgttacagtg ttgaaacaac ctagaacttt
    12541 atttatactg aataaagaaa atgaaaaaaa gaggtgacaa gatgatcgag cgctatcgta
    12601 caataaaaga aaatggacaa agtgagattg agatcaaaaa atcacgcttt atctgttctc
    12661 ttagacgaat agaatcagag gaccaagcaa aaagtttcat tcaagaaata aaaaaagaac
    12721 attggaaagc gaaccataac tgcagcgcct tcatcattgg agaaaaaaat gaaatccagc
    12781 gaagcagcga tgatggagaa ccaagtggaa cggcgggcgt accgatgttg gaagtcctca
    12841 aaaaaaatca actgatcaat gtagctgcag tagtcactcg atattttgga ggaacgaaac
    12901 tcggcacagg cggattaata cgagcataca ctcatgcggt ctctcaagca ttgtctgaca
    12961 ttggaatcgt agaaggtacc ttgcaacagg agatctctgt gatgatcacg tatccgcaac
    13021 taggaaaatt acagaattat cttgaactcc aacatttttt tataaaagaa atccaatata
    13081 ctgatcaagt tagcgtcatc tgtgctgtag atgaaaaaag tgctgaccac tttaaagaat
    13141 ccatcattga gctgctgaac ggacaagtcc aatttgaaga gggttctgtt tcttatcacg
    13201 agcaactgat aactgatcat atataaaaac aggtgattct gattctcttt tatttctaat
    13261 aaggaatcag ggatcacctg ttttttattt ttccaaataa aactcaaaac gatttcctac
    13321 gtattgcgta cgcacatact caaacggcgt accgtcttca aaataagaaa tctggcgcaa
    13381 acgaagaact gcatctcctc gttttacctc taaatagtca gcaattttct cagaagccaa
    13441 cacagcagta atcgtttgat tcgctggacc gatctttttc ccgccttttt cttctagcgt
    13501 gcgatacaac gattcagtaa tctctgcttt gctgtagcca tcgatcagct tttgtggaac
    13561 actggcaact tcaaaacaaa tagggacatc atctgcgaaa cgaatacgtt ccattcgcaa
    13621 gatcgtgtca tctttactca agtttagttt ttccatttca cttgagcttg gcgaagtcaa
    13681 gaaataagag attgtccgac tagacggtac tcgattttgt gattccataa tttctgtaaa
    13741 acttgttgca ccagacattt tttcttgtac tttttctcgc gcaacatacg tacccgaacc
    13801 gatctttctt tctaagattc cttcatctgc caagttttga attgcttgtc tcaacgtcat
    13861 acggctgaca ttgaatttca ctgctaattc tctttctgaa ggaagacggt ctccgacttt
    13921 ccatataccc tgttcgattt cattttttaa ttggtcatga atctgtatat atacaggtaa
    13981 ttgattcgac ataacgtaca cccgtccttt cgtttcttct attataactg gtatatacct
    14041 gattcacaac tgaaaaagat aaaggtttct atccatcaac agaggattta gacacacatc
    14101 gttctcccta ctcctattgt cagaaacaat tttttagata tgttcaacct agaaaataac
    14161 aaaagaccct aaaatcgatg aaaatgccga cttatggatc ttttgttatt attagcggtt
    14221 tttagccatc catttttttg tatgattcgt tgggcgtttt gattttacct ggaccgcttt
    14281 tccgcctttt ttcggaatat tcacttcaat attgtatttt tctgatttga ccaaatcata
    14341 taacttttca gctgcttcgt ctactttttc tttccatttc atgtggatcc ctccaattct
    14401 tgtagttaac ttaactctat cttaattcag cagaatcttc aaggaacaag gtagcaaagc
    14461 aaaaaaaaga gctctcgaac taacg
//
LOCUS       DAGCYL010000062        14354 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.063, whole genome shotgun sequence.
ACCESSION   DAGCYL010000062 DAGCYL010000000
VERSION     DAGCYL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.063"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(500..1003)
                     /locus_tag="MB216_001403"
     CDS             complement(500..1003)
                     /locus_tag="MB216_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBT4526245.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1362..2387
                     /locus_tag="MB216_001404"
     CDS             1362..2387
                     /locus_tag="MB216_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBT4526246.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2710..3588
                     /locus_tag="MB216_001405"
     CDS             2710..3588
                     /locus_tag="MB216_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBT4526247.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4064..4180
                     /locus_tag="MB216_001406"
     CDS             4064..4180
                     /locus_tag="MB216_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBT4526248.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5108..5446
                     /locus_tag="MB216_001407"
     CDS             5108..5446
                     /locus_tag="MB216_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526249.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5540..5770
                     /locus_tag="MB216_001408"
     CDS             5540..5770
                     /locus_tag="MB216_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526250.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6170..6541
                     /locus_tag="MB216_001409"
     CDS             6170..6541
                     /locus_tag="MB216_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBT4526251.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6815..7507)
                     /locus_tag="MB216_001410"
     CDS             complement(6815..7507)
                     /locus_tag="MB216_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4526252.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7510..8295)
                     /locus_tag="MB216_001411"
     CDS             complement(7510..8295)
                     /locus_tag="MB216_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4526253.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8532..9932
                     /gene="lacG"
                     /locus_tag="MB216_001412"
     CDS             8532..9932
                     /gene="lacG"
                     /locus_tag="MB216_001412"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBT4526254.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9957..10274
                     /locus_tag="MB216_001413"
     CDS             9957..10274
                     /locus_tag="MB216_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBT4526255.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10287..11960
                     /locus_tag="MB216_001414"
     CDS             10287..11960
                     /locus_tag="MB216_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBT4526256.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11974..13605
                     /locus_tag="MB216_001415"
     CDS             11974..13605
                     /locus_tag="MB216_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBT4526257.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
BASE COUNT         4572 a         2327 c         2923 g         4532 t
ORIGIN      
        1 tgttatttta tacttaaaaa ataacgacac agaaaatacc gtgtctcaga tttctctact
       61 acttctaaaa actactgttt ttttcctttg aaatgccttc aaaaagtagg atcaggcaga
      121 atatactggc agaatcaagg agaagggagc ctaaataagg agaaaaacca tagaaaatat
      181 aaagtaatta cttataaaac aagcttttta tagaaaaata atatacctga gtagggtata
      241 aattaagacg gaattccttt ctagattgag atattgacta ggaagaatgt taagatttta
      301 agcctttaag ctaaaaaaaa accctttttg ttaaataagg ggaatatagc ctgtcagttt
      361 caaactgcaa gtcgtcctgc tgcaataata tagtagatta gagatgaaaa tagatttttt
      421 taaaccaatg actgaataaa aagaccgtca tagatacaca ttggaatgtg tctattacgg
      481 tcttttttta cgaacgattt tattgaagac ggacagttcg ggtcaaaaat aaaaaagcta
      541 cgatcagaaa taagaataaa atgtatccca aaataatggc tagacggtct aacttatttg
      601 tcgatagatg tttgtttaga agttgatagg cccataaacc tatcagtcct cctgttgtat
      661 tagttatcaa atcagtgatg tccgctacgc caataccgaa taggaattga atcaattctg
      721 ccaagaagct tacaaggaaa ataatcccta attttttaga aaaagaaagt ctcttgaaat
      781 tgacattcaa aagcacccca aaaggaaaaa agacaatcaa gttataagtg gtctcacgta
      841 ataccgtcgt attttcaaaa gaaaaagtag aaaaagggac gagattgaca ttgctgtaag
      901 aataagtgag aaattgtgga agattaaatg atagcttgaa taaaatcaac cataatagaa
      961 agaataaata aatccctaat aaaaattttg atacataccg catagacatc tcctccattt
     1021 aaaaagtata ccatcgaaaa ccgctgaaaa atcataaaaa agtatgaagt ttaaggacca
     1081 acgtagttat cgtacgaaaa ttagaagctt ttttttgttg ggaatccggt ttctttaaca
     1141 aagctaagat agcgagttgc ttggaaaagt tgctatcttt tttctgtcaa aacgactcgg
     1201 aatgttcgtt ttttgttcta atttctccaa gttgtttact ttttgatact aagtttatga
     1261 aaaatcaaga aatagttgtt atactaggta atgctgacaa acgggattgg catttcatct
     1321 tggttcagct ttttttccaa ttactaggaa ggagggatag attgttcaag acttattttc
     1381 gtttcaataa aatacaagat aatccttttc gtatcatgaa tctgatgcta cttatcttta
     1441 ttgtattatg gttaggttac ataaatcatg attttttgat ttcaagtttt tcatcattag
     1501 ggatctttac ttttttctat tatcaaaaca taccgatgaa atatttgctg aaacgtatgt
     1561 ttctgatcgg ttgcggactg ttggttgctt tcgttttggg gatcttatcg acttatgttt
     1621 tatggcttga accatttgcc gtagctttgg ttgctttttc cagcagattc gtgttgcgat
     1681 tattccatat ttctaaacca ggtggcttgt tttttgcgat gctttctgcc atgggaacga
     1741 gcatgcagct gccaatcgct caattaccga tcgtctcact ttattttttt atgggtgtcg
     1801 tttttgccct gattgcggca gtgattacaa aattactgga ttcacggcct gagcagacaa
     1861 ttgaaaaagc aactttaaaa gaaagatttc acgaagagcc tttagtgatc attgattctg
     1921 ttttctacag cgctgcctta tttctttccg tttatgtcag tcacggcttg aatctgcata
     1981 atccttattg gttgaccttg tcatgtgcat cgattttgct agcagaaaac ttggatgcga
     2041 tgaagcatcg acaagtccaa tatctgattg gatcgatggg tgggttatgt gtgtcggcat
     2101 tcttgtcatt tgtgccgttt acgcagttgc agaccatttt tctgatcact tttctatatg
     2161 gaatcgcaca atttctagtt gctagaaatt atgcagttgc taatattttt cttaatccga
     2221 tggcgttgat gctatctaca cttattcgtg gagcatactt gatttctttg atcgaatatc
     2281 gttttttagg gatcgtcata ggaagtttca tcggtttagg tgttgcgtgg gtaatgacgg
     2341 ttggtttgca acattattta acggttgttc gtcaaaaatt agaatagaag gacaaatata
     2401 tgtgccaaaa gaggttatga caagattttt gtcacaacct ctaattcaag gtgttcaaaa
     2461 gccagattct cagcattaag tcaaattcac acaaaaatgt ggaaaggaac tttcaaaaaa
     2521 attttatatt tattctatca aaaaattttg tttatgatgg taactgaata aactttcctg
     2581 agatggtcaa tgctttcctt gaaagtctta cagatattct ttcttcatag aaagtttaag
     2641 ttttatagtt agtgtacaat aaaagtatgt ttataccata tggtgtccgg ataatcagtc
     2701 atttaaacaa tgaggttcaa agatatgaaa aggggaaatt tgatgaagaa aataagcaat
     2761 gaaatccaaa ttctggcaga aactttaact cttcccaaag tcgttataga gcaaatgatt
     2821 gtacaagcaa aaaacatcct tgatgaggtg tttgagcaat atagtcatga actaatggat
     2881 cctaataaag caaaggcgac tgcaaagaaa atagacaaag tctatgaaga ggaacctttt
     2941 gcagcattag ctatttatct atatgcagct tctcaaagtt ggaaaaaaat ttatcaacca
     3001 ttgaagattc caagaactat ctatttggca acaatgaatg cttttactag atttattcat
     3061 gaacatttcc aagtaacagg aagctatcgc tttgaccgtg gtttttggat ttggcattac
     3121 actagtggct tgatctttcg gattggtgaa ttggagtacg aaatgacata ttttccgaaa
     3181 gggtacacgg atctaagatt agaaggaaag cagtgccttt ctcttcatat tccttccgat
     3241 gcagatttat ctcagaataa gataagagat agttacgaaa gtgccattcg tttttttgaa
     3301 ctttattttc ctgattacca ttaccaagtg atgtacacag atacttggtt actatcaccc
     3361 aatcttacta aatggttgaa aaaagagtca aaaatctgtc tttttgcagc cgattatcga
     3421 ttattatcag ttgatgaaca ggacgatagt ggagtccctt ggatctttgg acgagtagat
     3481 gcccaaatac atgattatcc agaaagtacg agtttacagc gacaagcaaa agagcaattg
     3541 cttgcaggcg agcacattgg ctctggctta ggtattctta cttcataagt ttagtacttc
     3601 gaaatagttt aaaagagcgt gagacaaaag taaagaacac ttttgtctca cgctctaaac
     3661 acgtataaac ggcggaagca gaagcaactc cttcggaaat aagctgaaat tcacaaaaat
     3721 ttgaaaagca attttcgtga atttcttctt atttcttgga gttaaacgct tctgtcccag
     3781 cctctttttt agtgttctat cgaacacaca gtacgttcaa taggtaaact agctataaca
     3841 atagatatta aagttaacgg acaagagggc cttttttagg tagctaaaac gtaataatga
     3901 aaatttattt ccagagatag gagtcaatca gctagtctga tcgactgttt taacaagttg
     3961 actttcattt taacttttga taccatttaa aacacaaatc gtaattgttt cgatttgtgt
     4021 tttttagcat gactacaatc aaagaagaaa gtgggtcgtc catatggaaa aaacagatat
     4081 ctcaagtgca taccgccgct tgaaaagccc aaatatcaaa acacgaaaac gtgcgctgaa
     4141 gatcatcaaa gatgtgaaac ggaatagtgg gaaaaggtaa gaagaaggaa acttaattaa
     4201 aaaaatagcc gaaaaacgtt tttcggctat tttcaataat cggtttttgt gtaaggtttc
     4261 cgaaagaact gctgttgatt ttccattttt aacatatgat aaattggtag aatctttctt
     4321 ttcaaattga taccattccc taattccagt tatatagtta actacagaac cacaaagcac
     4381 gtacgtttat aaaagtatta tctgtagtta tttttcaatt ccctttcaac ctcttatttt
     4441 gaccagtagt attgatcact ttacctttta acacctttgt tcgtttttga aacattttta
     4501 gtcaagaatg gaaacgtaac tacataatca aaggttttgc gaaatatttc atgaaatatt
     4561 tcatgtagaa gcgagcaata cggttgcaaa taaccattat cgtttaataa gcagttacga
     4621 aagctcccaa tatctctaac gttcagctca ctccatttct tttcttcaaa aaaatcagtg
     4681 attcgtatct ctttctattt gtcgaaaaag ttaactttaa aaagtaacgc cgaaaaaata
     4741 tgcaaaaaaa taaaaaagca gttactattg ctagaataac atcaaggaat aataagtttt
     4801 ggttttcaga taatagtcga taagttgtgt attggaattt tagatacagt aataaagtat
     4861 ttttcatgaa attttgtgag aatcactatt acaagtatct ttaataaaat taatggttaa
     4921 ggaggaactt aaagtagaat gtaaatgaat agttgtagtc agtcgattgt gatactagac
     4981 tcaaagttgc aggaataagt ttttcaattt gtgattggaa gataaaaaat gaaaaagaag
     5041 ggcttttttt tatactttgt tgattgtagt gtttattttc cctctgttta gccaagtaac
     5101 attttctatg actcaaccag tttcaagtga ggaacatgat aacctttttc gtgctacaga
     5161 agagtttaag aaatatcagg aaaatattgt tccggagact ccattagcta gtgagccaat
     5221 tactgatatt gatggaaacc aaataggatg gtatatacag tacgaagtta tggtggtaaa
     5281 taatgactca atagtaaaaa atacagtagg cttagagcta acaccgattg aaaaggtgag
     5341 ttcattgtta acgtatattt atactgatga aagtgacgag actgagatca ttttaacgga
     5401 ttactcaaaa attgctactg ataaaaagta tatattgtgg atgtagacac tagtgaaata
     5461 ctttatgata tagaaattga tgaaaattta ctagatgtag cacaagaaat cgatcatcaa
     5521 aagcaggaag ctattcgata tgctggtaga aaagggaaaa gaaaatattt attacgtgaa
     5581 tgtagcaaaa gttagagaag atgaaaatga gtggaaagaa ttcaagagtc gctactcaat
     5641 taactcgact ccgacattta cagtttacag agaaggatcg atagaaaaaa ctgttttttg
     5701 gactaaagaa agtggaatgt ctttagctga agtagaagag tttttggatt atgttagtat
     5761 gcaacagtaa gggagttctt attatgaaaa attttgacac atattatgtt attgtttttt
     5821 cgatatcttt gctgttaata ttactaactt taatgcagta taaagagtat ccagagtatt
     5881 ttattttaac ttttgtatat ttagtaagta gctacacact tctatataaa ttcagaagtt
     5941 cttctagagt ttctaaagtt tgtttcttaa ttagcttatt gattctataa tatttttgat
     6001 ttatatagga aattaaaaat attatagaat caacactcag agaacgggtt ggtttttaat
     6061 agatttaata agtactgata atgaaatttc tcctggttgg gaaaacgaag ttggttaccc
     6121 tgtagaagaa ttttttcaag attactttgc ggagtatgcc gataggataa tgagagatta
     6181 cgtaacagat aaaactcaag aagtcatcaa tcaatcccag tcattaaatg aattatctga
     6241 taaggcgaaa gaagaagcag ttgccattat ttcttcaggg attagtgact atattgatat
     6301 gagtggtgaa atcaattatg gcgataaaga caaaattgta cgcatgataa aagcttttat
     6361 caaaactttt gatgtgaagg cagatcttaa gtttaatgat acttacctag taggaaaatt
     6421 aattaatatc cttgggacag agaaagatac ggaaacgttt atttcctctt tgtctatgtt
     6481 gtttacagat aagcattttt caactcagct tggtggagag gaactagtgg aaatctttta
     6541 accattacgc ccagcattaa taaaagtata tcaaaaaagt actcctgatg atttttaaga
     6601 ttggtttgag aaataatgtg aaaacactat ttttaatgtt aattatagat agagatgaac
     6661 gcgtcatatg gttaaaagaa tgaaaaagtc aaagatacac tgctatattt cactaaggat
     6721 atagcagtgt atctttcatg tcgtttcact ggcagttttt tataaaagga ggtgtgcatt
     6781 tcagttgctt ttctttggta gttagttgaa tcgtttaaga attaacggat gtggcagaca
     6841 ggtgctctgt ccaaaaacca gtaaaatctt tgatggttcc atctttattt agctgtgcct
     6901 gttcaaatac tgttttgttt ttaccgaatg gatcaattgc aataattgtt ccgatccccg
     6961 cacgtttggc agcaagtaat cctgagtaag catcttcgat aacaagacaa tcagccggag
     7021 ctaagctcag tttgtctgcc gctttaagaa aaatatccgg ttgcggtttc ccagggaaag
     7081 agccgtcgtc atagacaatc ttgtcccagt caaaccagcg ggctaaatcg aaaatatcaa
     7141 aataaaaagc aacattctct ttgacagtag ccgtggcaat cgttaatgga atctgttggt
     7201 cagtcagctg gtcaagagtc gcttcaagcc cttttgtcaa ctgaagctcg ttttcatttt
     7261 gcaagcaaag catacgataa tagctttctt tttcttctga caatacggcg atctcagagg
     7321 cggtcaaatg ctctgaaatg aaatgtttca aaatctccgt attcgtccga ccatgaatat
     7381 tttttaaaat atcctcgtct gataaaccat cttttgtatg atcatgtatc atatgcagcc
     7441 aagcagcttc atgcagatga gagtccaaaa acattgttcc gttgaaatca aatataatcc
     7501 ctttcatagt tacgctccta tagtggtatt ttcggttgaa tcagcaattt gcagatcgac
     7561 atattgtgca tagtgctctt gaacctcccg cgagatctgg cgatccgtaa tgagcagatc
     7621 gaaatcatgc aaattataaa acacataaaa atcatctcga ttgaattttt tattatcaac
     7681 gagtagatat ttcttacggg aattatttag agcgatctgc tgtgcttctc cttcttcggt
     7741 actgtaagta gagatcgctt cgttgtgaac gccatttgaa ctgataaatg cttttgtaaa
     7801 ctttagtctt cttaaattgt cgttggtgat cggtccgaca aatgcgccgg tatttttccg
     7861 gaaatcaccg ccaatcagta gaatttctgg tccatctgct tgagcaagaa tatcaaaaac
     7921 tggataatta tttgtaatga tccgaatttt cttgttcagt aaatgcgtcg ccaatagttc
     7981 gattgttgta cctggtccta agaaaatcgt ttctccctcg tgaatcaagg aggctgctgt
     8041 ggcagcaatt tctttttttt cttgaatctg cagagtggct ttttcagtgt gagaaagttc
     8101 ttgatctaaa gtgtaggaaa gactttgagc ccctccatgg acacgcagca gcttgcccga
     8161 tttttctaat tcatctaaat ctctgcgaac ggtcatatca gaaacatcta attgattgat
     8221 gatttcatta acggtaagga ttcctttttt attgaccatt tctacgatag taagcaatct
     8281 ttcttttttc aacattgtct tcacctctaa tttaaggata atctatcata taaacggaca
     8341 acagtcaatc taattcgaac aaaagtaaac atttagggtg gtttttatgt ttgttacaat
     8401 agattttatt gctgttttct ggtttttatg attatttgtg tttgtttttg ttgacaaatg
     8461 ttttggatgc gtttacaatg caatcagtta gaaagagcga aaactgatta tagatttgag
     8521 gagagatgta catgcttaaa ttaccagaag attttatttt tggcggagcc acagcagcct
     8581 atcaggttga aggagccact aaggaaggtg gcaaaggcgc tgttgcttgg gatgactttt
     8641 tagaggaaca agggcgattc agtcccgatc cggcaagtga tttttatcat caatatgcaa
     8701 aggacattga attgtgtgaa cgttttggtg taaatggtct aagattatcc attgcttgga
     8761 gccgcatttt tcctgatgga gctgggaaac caaatccaga aggaatcgct ttttatcatc
     8821 gtgtgttcga agaatgtaag aaaagaaacg taacgccgtt tgtgacgttg catcattttg
     8881 atacccctaa acgtttgttt gaccatggag attttttgaa tcgagaaaca atagaagcat
     8941 ttgtttccta tgccattttt tgttttcatg aattcaaaga agtcaaggtg tggagtacct
     9001 ttaatgaaat ctatccagtt gcaaccaatc aatatttgtt aggtgttttc ccaccaggaa
     9061 tcaaatatga ttttacaaag atcgtcgcct gtcttcataa tatgatggtt gctcatgccc
     9121 gtgtagtgaa ctatttcaaa gaaaacgaac ttccgggtga aattggtgta gttcactcat
     9181 tagagacgaa atatgcggca acagacgcgc cggaagataa gcatgctgcc tttttagacg
     9241 atgcattgtc gatccgtttc ttattagatg ctacctattt aggctattat tcaactgaaa
     9301 ccttgactgc tcttgatgag atatgtgaag caaatcaggc cagctatcat tttcctgaag
     9361 aagatttcgt tgaattgaaa aaagcatcca cgcgtaatga ctacttaggg atcaatcact
     9421 accaatgcca ttttgtcaaa gcttacgatg gagaaaatgc aatccatcat aacggtacgg
     9481 gtgaaaaagg gacctctgtt tataaagtga agggaatcgg agaacgtatt tataaagaag
     9541 gaattcctcg aactgattgg gactggctga tttatccaga aggtctatat gacttgttgc
     9601 tgcgaattaa aagcgattac ccacattaca acaaaatcta tatcactgag aacgggatgg
     9661 ggtataaaga tcagtttgaa gatggcatta ttatggatca gccgagaatc gactatttga
     9721 gagtctattt ggaatcctta agcaaggcga taacagccgg ggtaaatgtg aaggggtatt
     9781 ttttgtggtc gcttatggat cttttctcat ggacaaatgg gtataacaaa aggtatggct
     9841 tgttttatgt tgattttgaa acccagaagc gttacccaaa agaatcagct tactggtaca
     9901 agttggtcag tgaaaccaaa acgattattt aataagtgat aatggaggaa gaaaagatga
     9961 ataaagagga attacaaatg cttggttttg agatcgttgc atattcaggc gacgctcgca
    10021 gtacattatt gaaattattg aaagaagtaa gacaaggaaa ctttgaacat gttgattcgg
    10081 cgttgaaaga agccgacgaa aatttaaccc ttgcacataa ttcgcaaaca aaaattttag
    10141 cggaagaagc atctgggaaa gagatggaga tgggattcat ttttatccat gggcaggatc
    10201 atttaatgac cactttattg ctgagagatc tgattcaaga ctttattgta ctgtacagac
    10261 agaatcaagg ataggagaag aacgctatgg atggaatcat taaacaaatc gaaaaaggga
    10321 aaccattttt tgaaaaaatc tcacgtaata gttatttagg agccgttcgt gacggatttt
    10381 taacagcgat gccagctatt ttattttcaa gcattttcat tttgattgct gcggttcctg
    10441 agatttttgg ttaccaatgg cctgaatctg tctccacatg gctatggaaa gtctataatt
    10501 attcgatggg tgttgtaggg ttactcgttt cagcaaccac tgctcgctgc ttggctgatt
    10561 cgatgaatcg tcgcttaccg aaaaacaaag taatcaacac cacctctgtg atgctggcct
    10621 ctgtggtaag tttcctgttg ttaagcgtct ctccgattga aggtggattt tcaacggaat
    10681 atatggggac aaagggacta ctggcttcct ttgtcgctgc atttttaaca gttaatttat
    10741 ataagttttg tgtgctgaaa gatattacca ttaaaatgcc taaagaagtt cctggaacga
    10801 tttcgcaaac cttccgagat atttttcctt tctctttttc agtatttgcg gccgttttaa
    10861 ttgatttagg cattcgacaa atcttccaaa tctcatttgc agaagcaatc attacggtgc
    10921 tgcagccgct atttacagca gctgacgggt acttaggaat cgctattatc tggggagcga
    10981 tggcgctgtt ttggtttgtc ggtgttcatg gaccgtcgat cgtggagccg gcaattgcgg
    11041 caattatcta tgccaatgtg gaaaccaatc tgcaattgtt caattccggt gaacaagctt
    11101 ctaaagtact gacggtgggt ttagggaatt ttgtaggaac tatgggagga actggggcaa
    11161 cattggttgt tccttttatc ttcctactct ttgctaagtc taagcagttg aaggcagttg
    11221 ggaaagcttc ctttattcca gtaagtttcg ctgtcaacga accattgcta tttgcggcac
    11281 ccatgatttt gaacccttat ttttttattc catttttgtt aacacctgtt gtaaacgttt
    11341 gcctgttcaa gttttttgtc gatgtattgg gcatgaacag ctttatgtac gttttgcctt
    11401 gggcaactcc agcaccgatc ggtttggttc taggaacggg catgggtatc ttagcttttg
    11461 ttctagcagc ggttctaatc gtcgttgact tcctgatcta tctaccattt tgcaaagcat
    11521 acgatgctgt cttagtagag aaagaagcac aaaaagcaga ggtgatcgca accgaggaag
    11581 gtgcttctgc cgatgtacgt gaggaagtac ctgtggcagc gagtcaagtt atcagcgatg
    11641 caaataaaga aatcaatgtg ttggtacttt gtgctggcgc agggacaagt gcgatgttag
    11701 ctaatgcttt aaaaaaaggt gcatctgaat cgaaagcacc gatcagtgca atggcgggtg
    11761 cttatggttc tcattacgaa atgctgcagg attttgatat ggtggtctta gcaccgcaag
    11821 tcaattctta ctatgatgat atcaaagaag acaccgatcg cttaggaatc aagctggttg
    11881 cgacaaaagg agcagaatat atcggattaa cgagagatcc tgaaaaagct gttcagttta
    11941 ttctgaacca atttcaataa aggaggagcc ataatgaaaa tgggaatttg gttgcgtaaa
    12001 gctgttttat ttgcagctgt gttaggggct ttgggggctg tttcaaaaac tgttgaagca
    12061 tctgccagcg aatttattcg cggagcagat atttcgatcc tgtcagatat ggaaaaatct
    12121 ggtgcaaaat attatgaaga cgatatttca aaagatgcct tagagatttt aaaaaataat
    12181 ggtgtcaact atgttcgact gcgtttgtgg caggatcctt ataatagtaa tggagaaagt
    12241 tatggagcag ggacaaatga tctggaaacg acgattgacc ttgcaaaacg agcaaaaaat
    12301 ctgggactta aggtgctgct ggattttcac tacagtgatt tttgggttga tcctgggaaa
    12361 cagaatcttc ctaaagcatg gcaaggtctg acctttgaag aaatgaatac ggcattgtac
    12421 gactatacaa agaacgttct gtcggaaatg aagcagctgg acgtttatcc tgatatggtt
    12481 cagatcggca atgaactgaa tagcggaatg ctctggcctt atggcaagtc ttggggagaa
    12541 ggcgggggag aatttgatcg attggctgct tttttgaagt ctggtatcca agctgttcga
    12601 gacactcaac ctaaaacaac tccagtaatg ctccacttag ctgatggtgg cgatacagga
    12661 gcatttactt ggtggtttga tgaaatcacg agtcgaggtg tgtcatttga tttaattgga
    12721 gtgtcgtatt atccgtattg gcacggttca atgaatgaac ttcaaaccaa tatgaatgcg
    12781 atcagccaac ggtatggtaa agaagtgatc gtagttgaaa cagcttatgg gcatacaaca
    12841 gcgaatgccg atactatgcc aaatgctttt ggtgaagccg aagcagctgc ggggggatat
    12901 gaaccaacac cgacaggaca agcagagtat ttgctggatc ttgccgatcg gattcaggca
    12961 gtaccgaata atcgcggggc gggcttcttc tattgggaac ctctttggta caacggaaat
    13021 gtgagttggg caacacaagc aggtatgaat tatctcagtg ttcaatccac tatgggaaac
    13081 gaatgggaca accaagcgat gtttgatttt gctggaaacg ccttgccaag cttgcgtgcg
    13141 tttaaacaag caggggcgca aataaatcta gttaaaaatg caagtttcga aagcgatgga
    13201 ccaacgacca gtccttccaa ttggcaaacc tggtttcaac aaggaaccgc taagggtacg
    13261 atcaaaacgg aagcgggcac tttttttggg gactacaaac taactttttg gcaggatcaa
    13321 gcatacgagg catcagctta tcaaaatgtt agtggcataa gcaaaggaac ttacacattg
    13381 agtgcctatg taatgtctgg gggagagttt gatactaatc agttatatat caaaaattat
    13441 ggtggagcgg aactaaatac tgcggttgtt aaaacaagtg aatggacaaa aattacaatc
    13501 aaagacattc caattacaac aggtgtttgt gaaattgggg tttatagcaa ggcttcagga
    13561 aatgcttgga cgaacgttga ttttgtttca ttggtgaaac aatagactgc ggatcgtttc
    13621 gattcaagat cggaggttcc cttcagccac atcgttagcg tagaaggcag gaatcgaacc
    13681 tgcacctcgc ctttgccaaa aataaccaga aacgatttcc agtcattttt accaaaacga
    13741 gtaaagcacc agacttcatc tacactttgt attatgctaa agagagttca acgatttcgt
    13801 aagaagcaat gaaaaaattg caaaaagttg tgcagataac ataaagtttg tttaagaaag
    13861 ggtaacctta gaattccgtc gtgaatatga tttgagcact tagatggata gaaaaaagtt
    13921 ctcggaaaac agttcgtttt tcgagaactt ctttttatga taagaatgaa acaaattaac
    13981 ttatattata gtctcttttg gtagggaacc atcttatttt taatgaaaat caagcatttt
    14041 agctattgaa gaatgttgat ttatagggat tccccctcca tattatgttg gctctttatt
    14101 cccactctgt tttcaacgta aagtgtataa aacagaatgc gatactatca agggagttaa
    14161 aacaatatac agaacagtaa aacactataa ataaaaataa aagtgcaata aaagtgcaat
    14221 ttttgcacct aatcatactc tgtttatata tgaaaactag attctgaatc caatatttgg
    14281 ttaacgagag gcgttcgaat ttgaacgtct tttatatatt actttcaaat aaatatgagg
    14341 tagaatacta tcta
//
LOCUS       DAGCYL010000063        14184 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.064, whole genome shotgun sequence.
ACCESSION   DAGCYL010000063 DAGCYL010000000
VERSION     DAGCYL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.064"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(12..3686)
                     /gene="rpoC"
                     /locus_tag="MB216_001416"
     CDS             complement(12..3686)
                     /gene="rpoC"
                     /locus_tag="MB216_001416"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBT4526258.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(3744..7361)
                     /gene="rpoB"
                     /locus_tag="MB216_001417"
     CDS             complement(3744..7361)
                     /gene="rpoB"
                     /locus_tag="MB216_001417"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBT4526259.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8089..9057
                     /locus_tag="MB216_001418"
     CDS             8089..9057
                     /locus_tag="MB216_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4526260.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9075..9980
                     /locus_tag="MB216_001419"
     CDS             9075..9980
                     /locus_tag="MB216_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4526261.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9977..10783
                     /locus_tag="MB216_001420"
     CDS             9977..10783
                     /locus_tag="MB216_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4526262.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10919..11908
                     /locus_tag="MB216_001421"
     CDS             10919..11908
                     /locus_tag="MB216_001421"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBT4526263.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12225..12749)
                     /locus_tag="MB216_001422"
     CDS             complement(12225..12749)
                     /locus_tag="MB216_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBT4526264.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13036..>14184
                     /gene="scm"
                     /locus_tag="MB216_001423"
     CDS             13036..>14184
                     /gene="scm"
                     /locus_tag="MB216_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBT4526265.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTT"
BASE COUNT         4019 a         3066 c         2800 g         4299 t
ORIGIN      
        1 ttttaaaata tctattttgt ttgattgttc attgcgtctt ctgcagccat ttgcgcttca
       61 atatctgaaa tactgtagac attttcgctt gctacaccga cttcttttgg ttccatgtca
      121 cgataacgtt gcataccagt accagctggg atgatcttac ctataataac attttctttc
      181 aaaccaagaa gaggatcttt cttaccgcgg atcgcagcat ccgtcaagac acgagttgtt
      241 tcttggaatg atgcagcaga taagaaacta tttgtttcta atgatgcttt tgtgatacct
      301 agtaagacag gtcgagaagt tgcaggaacg cctcctgaga ctagcgtatt gtagttttga
      361 tctttaaagt ctgcgatgtc taataatgta cctggtaaga tatctgtatc acctggatcc
      421 attacacgaa ctttacgcag catttgacga accattactt cgatatgctt gtcgccgatt
      481 tctacccctt gcatacggta aacacgttgt acttcacgaa gcaagtagtt ttcaacagat
      541 aatacatcgc gaacttgcaa caattgtttt ggatcgattg atccttctgt caatggtgca
      601 ccacggtgga tcatatcgcc ttctgctact ttcatacgtg ctgtataagg aacagtatac
      661 gtacgtgtat ctgtttttcc tttgattgtt acttctttgg tacgtgttgc aggatcttct
      721 gagatatcga tgacatcccc tgttacttct gtgatcactg cttgcccttt cggattacgt
      781 gcttcaaaga tttcttggac acgaggaaga ccttgagtga tatcgtctcc ggcaacccca
      841 cctgtatgga atgtacgcat cgtcaactga gtaccaggtt caccgattga ttgagcggcg
      901 atcgtaccaa ctgcttctcc gacttcaacg tcagagccag ttgcaaggtt gcgtccgtaa
      961 caatgtttgc agacaccatg ttttgtatta catgtgaaga cagaacggat tgtcactgtt
     1021 tcgacacctg catcaacgat ttgttttgcg atgtcttcgg aaatcaattg gtttcctgca
     1081 atgatcaatt catttgtttc aggatgacgt acttctttac gggtgtaacg tcccactaaa
     1141 cgatcttcta atgattcgat gatttcattt ccctcgcgga tggcttggat ttcaagacct
     1201 cggtcagtac cgcaatcttc ttcacggatg attacatctt gggccacgtc aaccagacga
     1261 cgagtcaagt aacctgaatc ggcagtcttc aaggctgtat cggtcatccc tttacgagcc
     1321 ccgtgagttg agataaacat ttccaagacg gatagtcctt cacggaagtt cgaaatgatt
     1381 ggcaattcca tgattcgacc attaggagcg gccatcaaac cacgcattcc ggcaagctgt
     1441 gtaaagttgg aaatgttacc acgagctcca gaatcagaca tcatgaagat tggatttttc
     1501 gcttccaaac cttccatcag tttttgttgg atgctgtctt ttgcagcatt ccaaacagca
     1561 atgacacgtt cataacgttc gtcatctgtg atcagaccac gacggaattg ttttgtaata
     1621 gtttcaactt gtttatgggc attttcaatg atttcttgtt tttcattcag tacactgatg
     1681 tcagcgatcc ctactgtgat accggcatgt gtcgaatgtt tgtaccctaa gtctttcata
     1741 cggtcaagca tcttagaagt ttctgttact ttgaatcgtt tgaagacttc tgcgatgatg
     1801 tttcctaagt ttttcttctt gaacggtaat actaattctt gctctttgat atgtgcagga
     1861 atatcagtac cagcttcaac aaagtatttg tccggtgttt ggacggtcaa gttgaagtct
     1921 gttggttcat tcagataagg gaattcagga ggcatgatct cgttgaagat gatcttacca
     1981 actgttgtga tcatcgtacg ttctttttgc cattcagtaa atggtttttc acctaatgag
     2041 ttagggttca caccgatacg tgaatgtaaa tgaacatatc cgttgcgcca tgcgataacg
     2101 gcttcgtttt gatctctgaa gaccattcct tcgccttcgc gtccttcttc ttccattgtc
     2161 aagtagtagt tacctagaac catatcttga gaaggagtaa caactggttt accatcttta
     2221 gggttcaaga tgttttgagc agctaacatc aacatacgtg cttctgcttg tgcttcttca
     2281 ttcaatggaa cgtggacagc catttggtct ccatcgaaat cggcattata agcttcacat
     2341 acaagtggat gcaaacggat tgcgcgtcct tgtactaaaa ctggttcgaa tgcttggata
     2401 ccaagtcggt gaagcgtagg tgcccggttc agcaatactg ggtgttcctt gattacgtct
     2461 tctaaaacat cccatacttc gtcttcttga cgttcgattt tacgtttcgc atttttgatg
     2521 tttgaagcta gttcgcgttg aacaagctca cgcatcacga atggtttgaa taattcaatg
     2581 gccatttctt ttggaagtcc acattggtac atcttcaaga aaggtccaac aacgataacg
     2641 gaacgacctg agtagtcgac acgtttacct agcaagtttt gacggaaacg accttgtttc
     2701 cctttcaaca tatgagaaag agatttcaat gggcggttac ctggtccagt aactggacgt
     2761 ccgcgacgac cgttatcgat caatgcatct actgcttctt gcagcatacg tttttcattt
     2821 tgaacgatga ttccaggtgc attcaagtct aacaaacgtt tcaaacggtt attacggttg
     2881 attacacgac ggtaaagatc attcaagtca cttgttgcaa aacgtccacc ttctaattga
     2941 accattgggc gtaaatctgg tggaataaca gggataacgt ccataaccat ccagcttggt
     3001 tgatttcctg aagcgcggaa tgcttctaaa atatccaaac ggcggattgc acgtgtacgt
     3061 ttttgtcctt gtgcagattt caattcttct ttcaattcgg ctgcttctgc atctagatca
     3121 acattatcaa gtaattgttt aatcgcttct gcacccatag cagcttggaa ttcttgtcca
     3181 tattgttcac gtttttcgcg atattcgcgt tctgtcaaca attgtttttt ctctaaattc
     3241 gtatttcctg gttcgatgac tacatatgaa gcaaaataaa tgatttcttc caaagcgcgt
     3301 gggctcatgt ctaatacaag acccatacgt gaaggaatcc ctttaaagta ccagatatgt
     3361 gaaacaggag ctgctaattc aatatgtccc atgcgttcac ggcgaacttt cgaacgagta
     3421 acttccactc cgcatcgatc acaaacgatt cctttataac ggatacgttt gtattttccg
     3481 caggcacatt cccagtcttt tgtaggacca aaaatccgct cacagaacaa cccttcgcgt
     3541 tcaggtttta aggtacgata gttaatcgtc tcaggttttt ttacttcacc gtaagaccag
     3601 cttctgattt tttcgggaga agccaaacct atttgcatac tttcgaattt atttacatcg
     3661 atcaaaaggg gttccctcca tttcatttaa tggcaaaccg caagacggct gatcttcgac
     3721 cgccttgcag ctcatagacc ggattagtct aaattatctt ctgcggtttc aaagttttgg
     3781 atgacatcgt ctttttcatc ttccacttgt tcagcagctt tcttttctaa ttctttggca
     3841 gtttgctgtt cggcaaattt tgtcaaagca tcaacagtga tcaaatcgtc gtcttcatcg
     3901 tccatgtcgc gaagttcgat ctctgtttct tcgatatcca agacacgcat gtccagacct
     3961 agtgattgca attctttcac caatacgcgg aatgattccg gtacacctgg ttttggaatt
     4021 ggttcaccct tgacgattgc ttcgtatgtt ttcacacgtc cgactacgtc atctgatttg
     4081 tatgtcaaga tttcttgtaa agtataagcc gcaccgtaag cttccagtgc ccatacttcc
     4141 atttcaccaa aacgttgtcc accaaattga gctttacctc ccaatggttg ttgtgtaacc
     4201 aatgagtaag gtccgattga acgtgcatgc aatttgtcat cgaccatgtg agcaagtttg
     4261 atcatgtaca tcacaccgac agacacacgt ccatcaaatg gttcacctgt acgtccgtcg
     4321 tacagaacag ttttcgcatc gctggccata ccggcttcac gaactgtttc ccatacgtct
     4381 tcatcacttg caccatcaaa tactggcgtt gctacgtgga tacctaactg acgtgcagcc
     4441 atacccaagt gtaattccaa tacttgcccg atgttcatac gtgaaggcac ccctaaagga
     4501 ttcagcatga tatcgattgg tgttccatca ggtaggaatg gcatatcttc ttccggcata
     4561 atacgggata caaccccttt atttccgtga cgaccggcca ttttatcccc ttcatggatt
     4621 ttacgttttt gaacgatata aacacgaact aacatgttta cgcctggtga caattcatct
     4681 ccagcttcac gagtaaagat tttcacatcg tgaacgatac cgccgccgcc atgtggtaca
     4741 cgtaatgatg tatcgcggac ttcacgagct ttttcgccaa agatcgcatg caatagacgt
     4801 tcttctgcag ataattctgt tacccctttt ggcgtgactt tccctacaag caaatcgcca
     4861 tcttggactt ctgcaccaat acggatgata cccatttcgt ctaaatcttt taatgcgtct
     4921 tccccgacat tagggatttc gcgtgtgatt tcttcaggtc ctaattttgt atcgcgggct
     4981 tctgattcat attcttcaat atggatagaa gtgtatacgt catcttttac caaacgacgg
     5041 ctcatgatga tcgcatcttc atagttgtac ccttcccaag tcatgaaacc aactaaaacg
     5101 ttttggccta gagccatttc gccttgttcc atagatggtc cgtctgccag agtatcccct
     5161 ttttcgactt tctcacccaa atgaacgatt ggacgttggt tgtagcttgt tccggagttt
     5221 gaacgacgga attttgttac atcgtattta tctaatgcac cgttgtcgcg gcgtacgcgg
     5281 atttcagaag catcaacgta ttcaacgaca ccgtcatgtt tacataacaa agcagctcct
     5341 gagtcatgcg ctgatttgta ttccatacca gtaccaaccc atggtgcttg aggattgatc
     5401 aacggcactg cttgacgctg catgttggca cccatcaagg cacggttgga gtcatcgttt
     5461 tccaagaaag gaatacatgc tgtcgcaacg gcaactactt gtttaggtga tacgtccatg
     5521 tagtctactt tatcaataga tacttctaag ttttcacttt gagcacgagc cataacaacg
     5581 tcttgagcaa atgttccatc ttcattcaat gggctgtttg cttgggctac gatgtaatgg
     5641 tcttcgatat cagctgtcaa gtagtcgatt tggtcagtaa cacgacctgt ttcacggtct
     5701 acacgacgat aaggtgtttc gataaatccg aatttattta ctttcgcata actggacaaa
     5761 ctattgatca acccgatgtt tgggccttca ggagtttcga tcggacacat acggccatag
     5821 tgggagtagt gaacgtcacg aacttcataa ccggcgcggt cacgagtcaa tccgccaggt
     5881 cctaaggctg aaagacgacg tttgtgtgtc aattcgccaa gagggtttgt ttgatccatg
     5941 aactgtgaca actgagaaga accaaagaat tctttgatac ttgccacaac tggacggata
     6001 ttgatcaatt gttgaggtgt caatgtttca gtatcttgga tcgacattct ttcacgaacc
     6061 acacgttcca tacgtgctag cccgatacgg aattggtttt gtaaaagttc accgactgaa
     6121 cggatacgac gatttcctaa gtggtcgata tcatcgacat tgccgatacc ttccatcaag
     6181 ttcaagaaat agctcattga agcaatgatg tcggctggac gaaccgtttt tacagctgct
     6241 tctggatagc cgtttccgat cacgtttact tcacgttcag ggtctcttgg tgaaaatact
     6301 ttgatgactt ggacagtcat tggatctgtg accacaccgt cttctgatgg atagtaagtc
     6361 actgaattta ggccattatc aatgaaagga gctagtgttt ccatgacttg gtgagtcaga
     6421 actgttcctt tttcaacgat gatctcgcct gtttctggat caactaatgt ttcagctaat
     6481 gtcagattca atagacgtgt tttcaaatct aattttttgt ttactttgta acgaccaacg
     6541 tttgctaagt cgtaacgttt tggatcgaag aaacgagcat tcagcaagtt acgtgagctg
     6601 tctgctgttt ttggttcgcc tggacgcaaa cgttcgtaaa cgtctttcaa accttcttct
     6661 gttcttgagt cgcttgcgtt tttatgcaag tctttttcga ctgtattgcg taatgtttcg
     6721 ctgtcgccga agatttcaaa gatcgtatca tcggaaccaa atcctaacgc acgaactaat
     6781 acggttaaag gaatcttacg tgtacggtcg atacgtacat aagagatgtc ttttgcatca
     6841 gtttccattt ctagccaagc tccacggtta gggataacag tagaaccgaa tccttctttt
     6901 ccgtttttgt cgacttttcc atggaaatag acacctggtg aacggactaa ctgagaaacg
     6961 attactcgct ctgccccgtt gatgatgaat gttccttgtt ctgtcattaa tgggaaatca
     7021 ccgaagaata cttcttgtgc tttgatttcg ccagtctcgc ggtttgtcaa acgcaatgtt
     7081 acatgtaatg gtgcagagta gtttgcatca tgcgcacgag cttctgctac tgtatacttt
     7141 ggttctttca attcataatc tacaaattcc aaagatagat tgccattgaa gtcatcgatt
     7201 ggcaaaatgt cttcaaacat ttctctaagt ccctcatcta agaaccattg gtaagagtct
     7261 gtttgaattt cgatcaaatt tggtaattcc aacacttcac tgattcttgc gaaacttcta
     7321 cgttcgcgat gttttccgta ttttactacg tgtccagcca agctcttcac ccctctaaaa
     7381 tgtcattcaa aaagactacc cgatcaattg ttacaataat atttcgaata ttatttttag
     7441 gtaacaacag acattttttg cggcttttag gcaaaaaaaa accaaaaata gaacattctc
     7501 ttgaaacctt acttcgtgag cttctacttt tgtttcctta tattaaagcc gaaacggaca
     7561 atatttcgtt tctgcctttt cttcatcaga tagtttttgc aacttcatat actacactta
     7621 ttttctataa aagtcaacac ttcctggcgt ttcttctgct ttttcgcaac attttcttaa
     7681 tgttttcaat tttatgtttt caattatctg acaattctta attgacagac ctttttatct
     7741 cggttatact ttagaaaatt cattttgtgg aggaacttct atgcgaacca acatcaacca
     7801 aatcgcacca acacaactga ataactttta ctttagctac tttagatagg tttgtattca
     7861 tcatgatgga tacaaacgtt aaacgacagt ttgtatctat gatggctaaa gaactttgta
     7921 ttgcagcaaa aagccacgta gacaaaatgc taagtggctg agcagtgaaa atacaaagta
     7981 aaagtatcaa gcattctact ttttcaattt attttccgac ttttcaatcc atttggacat
     8041 tttgtctgaa tggatttttt gtttcccaaa attaaggagg aaaaagaaat gaaaaaaaat
     8101 ataatcatcg gattaggcgc tgccctgcta ctagctttag gaggttgcgc acaaaaagac
     8161 aattccgcag aggcatcaga tggacaaaaa acaattggcg tattgcaact tgtacaacat
     8221 aactcgttag atgctgccta taaaggattc aaagaaggat tagcagaaga agggtataaa
     8281 gaaggcgata atctaacaat cgattatcaa aatgcccaaa acaatcaaga caacctgaaa
     8341 agtatgagtg agaaactcgt caaagaagat cctgatttgc tacttggaat cgccactcca
     8401 gcagcacaat ctttattaaa tgaaacaacc actatcccga tgactgtcac agcggtcaca
     8461 gacttggaac aagcccattt agtcacatcc aatgaaaaac ctggcggcaa tgtgactggc
     8521 acgacagata tggtgccaat cgaaaaacaa atcggcttac tgttaaatat cgtgccagac
     8581 gccaaaacaa tcggcatcat gtataacaac ggtgaagcaa actctaagat ccaagctgat
     8641 ttggcagaaa aagaattgaa aaaagcgggc gtcaaagtca aagtactgac agcaaattcc
     8701 acaaatgatg tccaacaagt gacgaaaagt ctagctaaag atgtagatgg aatctatatt
     8761 cctactgata acacgttcgc aagtgcggca agtgttgtcg gtgaagtcgc aaaagaaaca
     8821 aaaacacctg tcgttgctgg cgcagttgat caagtcaaag acggcggtct agcaacagta
     8881 ggaatcgact acgaaagctt gggcaaacaa acagggatca tggcagcaaa aatcttagac
     8941 ggcaagaatc caagtgaact tcctgtcgaa acagcgaatg atttggaact gtatgtcaat
     9001 aaagacatgg ccaaagcatt aggcatcaat ccagacagca tcaaagaagc tgaataaaac
     9061 acaggaggat tctcatgaca aatttgctct ctcttttctt atcatctgca tcgcaagggg
     9121 tcttatgggc cctacttgcg attggtgtat acgtcacttt ccgtatcttg gacattgccg
     9181 acttgactgc tgaaggaagc tttcctttag gtgcagggat cgcggcagta agtattacaa
     9241 atggctatca tccattgaca gcttgtcttc taggcttttt agggggtgcg gcagcagggc
     9301 ttgtttccgg actgcttcac acaaaattga aaattcccgc ccttcttgca gggatcatca
     9361 ccatgactgg tttatattct gtcacatccc gtgtgatggg tgcgccgaac atctctttat
     9421 tgggtcagcc aacgatcttt cattgggtcg agtcattcgg cataacaaaa gacaacgctg
     9481 tactgatcgt cggtctgctt tttgctttga tcgttgtatg tttacttgtt ttgttcttac
     9541 gtacagaaac aggacttgct attcgtgcaa ctggcgataa tctggcaatg agcgaagcaa
     9601 acgggatcaa cacagataat atgaagatca tcggttatat gatttctaat ggatgcattg
     9661 ctctttctgg ctccttactt gcgcaaaaca acggctttgc tgatttaaat tctggtattg
     9721 gaacgatcgt aattggactt gcttctatta tcatcgcaga agttttgtta cgtaaccagc
     9781 cactatttct ccgtctcttg actgtcgttt taggtgcggt catttatcgt tttattttag
     9841 cgttagtctt cgaactgaat gttgaaccaa gtgattcgaa acttgcttct gctttcgtgc
     9901 tagtgatctg tctttctttc ccgcagatcc agcaaaaatt ccatttacct caaaaaaaga
     9961 aaggcggtag tgtcaaatga atgacgtctt aacattggaa aatctccatc atacctttga
    10021 aaaaggaacg atcaacgaaa atcatgtcct gaaaggcatt aatctttcct tagatcccgg
    10081 cgacttcgtg acgatcatcg gtggaaatgg ggctggtaaa tctactttgc tgaacagcat
    10141 cgccggtaca ttttctgtcg atcagggtca aattttattg aatggaaaaa atatcacgaa
    10201 aaaatctgtt gtcgaacgtt cgaaatccat cagtcgcgta ttccaagatc ctaaactagg
    10261 gacagccgtt cgtttaacgg tagaagaaaa tcttgctctt gctatgaaac gaggaaaaaa
    10321 acgcggcttc ttccgtggtg tcaaaccaca agaccgaaca tttttcaaag accagctggt
    10381 tcggctaaat ttggggttgg aaaatcgttt atcctcagaa atcggtttgc tgtctggagg
    10441 acaacgtcaa gctattacgt tattgatggc cacattacag cgtccagaac tgatcttatt
    10501 agatgaacac actgcagcgc ttgaccctgc tacttccaaa acggtgatgg aattgacaga
    10561 aaagctgatc actgagcaga atctcactgc ttttatggtc actcatgaca tggaagacgc
    10621 gatccgctat gggagtcgac tgatcatgct gcatcaagga aggatcgtcg tagatatcca
    10681 gcaagaggaa aaacagcagc tcacagtgcc ccaattatta gcgttgttca aacaaaacag
    10741 cggcacggca ttaaaagacg atcaagtact tttatcttca taatcagaaa gaggatggga
    10801 caaaaaatga acgcccaccc tcttcttttt tctctttcct tctttcgttt ttgctttatg
    10861 tatcaaccgt tgtttcttcg aaaactattt tgttctctcc ttcttcctga tagaatggat
    10921 gggaaaggag cttgatgcca tgacaacgaa agacaaggta cttcaattat tgaaaaagac
    10981 accagatttt ctttccggag aaaagttggc acagcaactg gaagtttccc ggacaagtat
    11041 ttggaaagcc atcaaagaat tggaaaaggc aggctatcgt tttgagcacg ggcctacagg
    11101 gtatcgttac ctgccctctg acgtactaga tgcaaaagaa atccagcaag agctacggtc
    11161 gtttattcct gatttgaaag tacaaacgat gttgacttct gaatctacga tgaaagatgc
    11221 caaactggca gcagctcaag gagattctgt ttcgaagtta tttgttgctg atacacaaga
    11281 acaagctcgt ggacgttttg gacggccttt tttcgctgaa cctggacgag gaatctacat
    11341 gagtctcttg ctccatccag acaaacggtt tgatgaattg cctcaatata cgatcatcgc
    11401 agccagtgcc tgtgtgaaag ccattgaatc tgtaaccggt cgttctgctt cgatcaaatg
    11461 ggtcaatgat atctacatgg atggaaaaaa agtctgtggg attttatctg aagcagtcag
    11521 tgatatggaa agcggcagga tcagtcatat catcattggg atcgggctta atttttctgt
    11581 tcctcaaaat cattttccag ctgatctcca agaaaaagcc atctctgtct ttcccgacgg
    11641 acaagctagt gtcacaagga atcagctaat cagcaaaatc tggcaagaat tgttcactta
    11701 tctttctcac ccagaaaaag cgttggactt ctataaagaa aaatcattcg tattgggaaa
    11761 aaccgtgtcg ttcaatcaaa aaggaacgac ttatgtcggt cttgcaaaag aaatcggtga
    11821 tcatggcgaa ctgatcgtcc aaacaaatga acagaagatg agtttgtctt ctggcgaaat
    11881 cagtctcaca tctatccgta cgcaataatt atcagatttc acaaaaggac acctgatctc
    11941 atacagtgtc cttttttctg aattctcttc aaaagactct ccttattcta tcagctgaca
    12001 gtttctctat cctctagaaa aaggtattct aaaaatatct ataaagcatg gtacactttc
    12061 tttaaatgaa tcaatgcaaa aaacaagaga gtcatcagtt tacacgatca aaagagatag
    12121 caggccgctt cctttttcaa tcttatagat agaccatggg taacgataaa aacaaaaaag
    12181 aacttgcgac ttattttgcc gtaagttctt ttttagctag tgccttaatc tcttaatccc
    12241 aacaaacgtc tcgcatagat cacctgacca gagtgcatag cagcatcatc aataatagaa
    12301 acgagacgca ttcctcgtgt gactggtggt gtccagttct cgtcaattac ttcatctagc
    12361 tcggaaggtt ccagccttct taagtaagaa atagcaaaat cagcagctcc tcgcaagtat
    12421 cctagcagat cttctatttg attagcttgg atctgctttg cttgttccag actatgcttc
    12481 caatcttgtt cgttatctgc tatcgtcatc ggaaaccggc tttcccagtc ttgactatgc
    12541 caaattggag aagttcctgc taaatcagca atctggaaat ctaattctcg agcggtatgc
    12601 caagccagcc aagtcattga tttgatctgc ggggctctat cttccactgg aaaacgattg
    12661 gcctcctcaa tagagacgcc ttcaaacaaa cgttcaaacc gatcgtatgc ccgatccaca
    12721 ctctcgcata gcatatctaa ataatccatt tattttcctc ctttttattt atcctactta
    12781 ctttttccta attctaggat acctacttat atgttactac acacccttat tttttacact
    12841 ttaacagtca ccatcatcaa tagtttggag attgaaagaa aaagatagtg tcgaactatc
    12901 aattgaacaa gaagcaacaa tatgaacgtt tcattaatat tctttaaata aacaatgatt
    12961 tttcaaacga acagtttaag actaggagaa aaagagtata ataggaaaat gtttgaaagg
    13021 aaaggaaggg gaaaaatgaa aaaaagggca agtttactag tcctagttgc tttgttgttc
    13081 aatatctttt cgtatggtgt ggccgtttca gcggaaacat acattcctaa agaccctggg
    13141 aatatttata atgatctaac tggtaactat ggcttgttag gaattgctag tcaattccac
    13201 gtatttgcta aaaataagac aactgtgaat gcacatacag acggaaatat agcaacacat
    13261 gaacttgatg caaaaaataa ttttggtaca gagcttcatg aaggtttatt gaaccaagaa
    13321 ataaattata tacaaaaaac tgattctttc gctaattctt caggcatacc tacaggtagc
    13381 acaagaatga ataaatttgt cgttggcagt aacgtggatg tagcacttca agatggtcat
    13441 cctacagtca atggatccag gcttgatcac ttaactccag atgattttta taaagatcgt
    13501 gctgggcatg aatatattga tttcgagcag gaatttgcta aattgaatgt agcagcaaat
    13561 gatcttgcaa cgatcactcc agctaaaaca tatactgctg cagattttcc agacatgaac
    13621 aatcggacaa tcgatttaac tgatttatca gataccgggt ttttattggt aaatatcgat
    13681 gcagaagtgt taacgatgaa tacgccactt caaatcatta atccaaatga tcaagtagtt
    13741 gtatttaatg tcatcaacag ttcttctgca ttgaacgtac agtcaccaat caaatacaat
    13801 gatcgttcaa atcatgaaac agaagatttt tcggatgcga atatctcttg gaattttgga
    13861 aatgagatga ctgatttgac aatcagtgct ccttttcaag gaacaatcct tgcgcctaat
    13921 gctactatcc gcattactca aaaccaagac ggaacgatta taggaacaaa tgtgatttta
    13981 gatgcagcta ccaatcgttg ggatcctaac gaaatattta tcagcgacaa tggtacagat
    14041 acgacagaca caagcgacag tacagataca tctaccacaa gtgatagctc agattcatca
    14101 acttcaagcg acagtacaga tactacctct accacaagtg atagcacgga tacatcagct
    14161 tcaagcgaca gtacagatac tacc
//
LOCUS       DAGCYL010000064        14103 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.065, whole genome shotgun sequence.
ACCESSION   DAGCYL010000064 DAGCYL010000000
VERSION     DAGCYL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.065"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..237)
                     /locus_tag="MB216_001424"
     CDS             complement(<1..237)
                     /locus_tag="MB216_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4526266.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQ"
     gene            complement(716..1435)
                     /locus_tag="MB216_001425"
     CDS             complement(716..1435)
                     /locus_tag="MB216_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBT4526267.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1484..3130)
                     /locus_tag="MB216_001426"
     CDS             complement(1484..3130)
                     /locus_tag="MB216_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBT4526268.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3309..3899
                     /locus_tag="MB216_001427"
     CDS             3309..3899
                     /locus_tag="MB216_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4526269.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3991..5496
                     /locus_tag="MB216_001428"
     CDS             3991..5496
                     /locus_tag="MB216_001428"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBT4526270.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5580..6932)
                     /gene="celB"
                     /locus_tag="MB216_001429"
     CDS             complement(5580..6932)
                     /gene="celB"
                     /locus_tag="MB216_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBT4526271.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6932..7450)
                     /locus_tag="MB216_001430"
     CDS             complement(6932..7450)
                     /locus_tag="MB216_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526272.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7466..7792)
                     /locus_tag="MB216_001431"
     CDS             complement(7466..7792)
                     /locus_tag="MB216_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBT4526273.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7805..9784)
                     /locus_tag="MB216_001432"
     CDS             complement(7805..9784)
                     /locus_tag="MB216_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBT4526274.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9805..10119)
                     /locus_tag="MB216_001433"
     CDS             complement(9805..10119)
                     /locus_tag="MB216_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBT4526275.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10287..10580
                     /locus_tag="MB216_001434"
     CDS             10287..10580
                     /locus_tag="MB216_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBT4526276.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10658..11770)
                     /locus_tag="MB216_001435"
     CDS             complement(10658..11770)
                     /locus_tag="MB216_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBT4526277.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            12157..12336
                     /locus_tag="MB216_001436"
     CDS             12157..12336
                     /locus_tag="MB216_001436"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526278.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12530..12700
                     /locus_tag="MB216_001437"
     CDS             12530..12700
                     /locus_tag="MB216_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4526279.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(13124..14071)
                     /locus_tag="MB216_001438"
     CDS             complement(13124..14071)
                     /locus_tag="MB216_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HBT4526280.1"
                     /translation="MQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAYQLVETQ
                     APTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQ
                     DANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAV
                     VELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDG
                     LAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTS
                     SGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4383 a         2894 c         2415 g         4411 t
ORIGIN      
        1 ttgatccgaa gaaaataaaa atttgaaatt ttcccaaccc acccaaggac tttccctcaa
       61 gctagccaag aacccatctg ctgaaatatg aaaatttttg aatgctacga cattcgccat
      121 tacaggtatg taaaagaaaa aaatcatcca aagcataccc ggtacagcca tcaggacaag
      181 tgctttataa cgccatagat tcataaaaaa atgattgaac ccctttcgtt ttttcatcta
      241 aaaacccctt tcgattcatt cgcaacgtaa ttatagcgct ttcaataatg agaataaata
      301 aacaaagtat agggaaaatg aaacaaagta taggagaggt tgtgaaatca gcgttttggc
      361 ctgagtatta gagaaaaaaa cgggaaaata gttcctcata ttttgccgtt ttttgagcta
      421 ataccgaggg tcagctgatt gaacaccgtt tatcagaggt tgtgaattca gcgttttggc
      481 ctgagtatta gggagatcgt gtccttacta tctaactctg agtcacaagg aacaatgggg
      541 actgtaacaa aagtcttgtc acagtccctt attccgaaca aacggtgttc cagaagtagc
      601 ttctacggaa ataagccgaa atttcacaaa aaagccatcc gaaagtcaga cggacaagtc
      661 tgcttttcag atggcgctac aactactctg cttattcttt tagctatcca ttcaattatt
      721 tttctctcac ttgttctagc tcttctttgg tcatgggtcg atattgccct ttattcaaat
      781 ctttaggcag ccaaaatttt cccatacgga ttctttttaa atataagact tcttttccta
      841 ctgctttgac cattcgctta acttggtgaa actttccttc atgcaaaatc agacggataa
      901 ttgaagtttc tgtctcttga tctacttgat caatttggag ttttgccggc aatgtctgct
      961 catcgccatc aataatcagt ccttctgcaa ataaacggat atcttcttct gtcattatac
     1021 cgctaacctc agctagatat tctttctcca catgtttttt aggtgatagt aactgatggg
     1081 caaacacccc gtcattcgtt aaaatcaaga ggccctctgt gtctttatct aatcggccga
     1141 ctggaaaaag atcttcccga taatcctggt cttccaataa atcgattacg gtttgatcat
     1201 gattatcctg agtagcagaa atgactcctg caggtttatg caaaatgtag taaaaatctt
     1261 tttgatacgt aatgatttct cctaaataag taaccgcatc ttcgaattca tttacttgcg
     1321 ctttatcgct tttcaccact tgtccattga cttcgatcgt tccttttttg attaatcctt
     1381 tgacttcttt gcggctgcca agtcccatat ctgctaaaaa cttatctaaa cgcatgcgtt
     1441 tcccccattt caaaagagca tcaatctagc tcttctgatt cacttatttt atttttaatt
     1501 tatgtcgtaa acgcgctatt cctggtccta gtagtctttc tgccaagcgg attttcaaag
     1561 ctaaataacc atacactaaa gcccctatac ctgctactac aagaatcaac aatagagact
     1621 ggaatttgct atcttcgttt aaaaaagcac caaaaatcat ctttgtgatc cacgaagcca
     1681 aaagcataat caaggtaaga ataaagatca gcaatgtcct gcgccagaca aaacttggtt
     1741 taaagcgggc tacttcatgg attctcttta agatcaaggt acaagacaca gcaaatccga
     1801 tcattgtcgc caataaaggg ccgtatactt caaataaacg aatggctggg tattgaagaa
     1861 ggagtttcac tagaaaccca ataaccaaat aattaattgc cgccttgttt tcaaacattc
     1921 cttgcagcat gttggaaact agcatatata gtcctaggaa gagtcctaca aaacttgctt
     1981 ggatcagtac attacttcct agagaatccg gtgtataaaa taatgtattt agcggatagg
     2041 caagtaacat aacaccaaat gtcgccggaa acataaaaaa tgaaaacaac tgtaaatttc
     2101 cgcttgtcaa ttttgccaga cctactcgat ctttcaacgt aacagcttct gtaatcaacg
     2161 gtaacccagt agacgcgatc gatgttgcca atgcaataac taccatcgtc aacttatctg
     2221 gattggcact aaaaatcgaa aaaagatcca gtaactgtgc attggaatac tcggtggatg
     2281 cagacatgat cttcataaaa gtgaactgat cgactaattt gaagatcgtt acccctgaac
     2341 ccacaataat aaacggaatc gcttctttaa cggtatctaa caacaattcc ttcgttgcaa
     2401 tatttatttt gttttcgctg tagtgaataa acgccgatgt atacgcttga tgtttcttca
     2461 aaaaatacaa aaggacaaga atacttgcta gcataccgat aaatgccgcg aaagttgatt
     2521 gcgtgaccgc cgtcacatag tcaccatcca tgactttcat gatgatgaat gtagccaata
     2581 gcatataaaa aatacgtgcc acctgttcta caatctgcga cagcgcataa ggcatcattt
     2641 cttgattgcc ttgcaaatac cctcgtatca cactcataca aggaaaaacc aatacagcga
     2701 tactcaaaga acgcatcgtt ggaatcagtt cttctccgcc accagaagca tgagctagcc
     2761 aaggagaggc gatgtacatg aacaaagcaa acaacaggcc taacccgccc atcatctgta
     2821 atgcacggat aaataatcgt cgtgaagtgc catattcatt caatgaatta tagcgtgcag
     2881 tttgttttgc aatcgcagca ggtattcctg ctgtagagat cagtagaaac aatgcatata
     2941 tattgtatcc catattgaat aatccattgg cagccttagc attttcgccc atccaagcat
     3001 accagggaat gatatagaca gcgcctagca atcttgaaat gatattactg gcggtcatcc
     3061 aagcagagcc tcgtgccatt ttttcttgcg gtgttaattg atctaatgct ggttttcttt
     3121 gattatccat cggtcagatt ccaactttct acaaaacatt actatcattt taattggtaa
     3181 aacagaaact ggcaatcgat tttatataac ttttataaga acaattgttt ttttgaattc
     3241 ggtttcgttt ttttggtaaa gtaaagaaat atacagcctt ttatggctaa catttatagg
     3301 agatatcaat gacacaagaa ataaaaaaac tacccgttat atttttaggt cttttcttta
     3361 tcacagttgg attgaattgg ttcttgctcc ctcacgatat agcttccgcc ggagtgggcg
     3421 ccatcggaca tttagttgaa acggcctttt ctatccaacg ctcagttact gtttggagca
     3481 tcaatcttat gatgctgctt ctagccgctg tcctattagg aaaatcagta ttttttaaga
     3541 ctgtagcggg aagtctgctt tttcctgttg ttttagagat tgttccaaaa gtggaaatga
     3601 tctcctcaca tttcttttca ctgatttcag gaagtctttt gttcagtctt ggggtttaca
     3661 cattgtacac agtcggggcc tcaaatggcg gtataacgat accgccgatt attttccaca
     3721 aattttttcg gcttccaatg ccgcaaggac ttttacttac caatagtctt atcgtctttt
     3781 tgaattatta tgtttttgga ttgttggaga cactattcgt tctcctatct attacagtca
     3841 gttcattgtt tatgaaaata ctcatccgct tgtcccctgt gtcaagaaaa tttgaataaa
     3901 gctggatttt gtcatagcgg aaacaaacga tatattaaaa agagtgtgct ataataaacc
     3961 aagaaattga ataaagcgag ggatcaatca atgagcttct ctttagaaga catccgtcat
     4021 ttactattaa aagaaaatct attaaaagaa tttgtttcaa caagaggctg gcatcttgat
     4081 gtaccagaaa aagctgaatt tcttcagtta agttatgatt caagaaaagc tgatgcttct
     4141 actttgtttt tctgtaaagg aatgaatttc aaagaagaat atttggattc agcaatcgaa
     4201 cagggtattc aatattatgt atcagaacaa ccatacgaaa attcagcagt aggaatcatc
     4261 gtaacggata tccgtaaagc aatggcgctt cttgctatgg ctttttacga ctatccgcaa
     4321 aataaattga aagtgatcgg attcactgga acaaaaggta aaacaactgc tgcgtacttt
     4381 acaaaagcaa tcttggatca cacaacaaat aaaaaaacag cattgctttc tactatgaat
     4441 acaacgttag atggcaaaac atatttcaaa tcccatttga caacgcctga gtcattagat
     4501 ttatatcgta tgatgtcaga agcagttgaa aatggtatga ctcatctagt gatggaagtt
     4561 tcttcacaag cttacaaaac acaacgcgta tacggcttga ctttagatgt cggaatcttc
     4621 ttgaatatct cgcctgacca tatcagtccg atcgaacatc cgacatttga tgactatttc
     4681 tattgcaagc gacaattgat cttgaattca aaaacagttg ttttaaatca tgaaagtgac
     4741 tattttgatt tacttaggga aacagccgat ctattccaga ttccaacgat cacttatggt
     4801 cgttcggaag aaagcaatta tcaagtgatc cgttcagata aaggtactca cggttttact
     4861 ttatcttctc atgaagatcg cttagctatc tgcgatactg cttatgatat tttgctggct
     4921 ggaggattca accaagaaaa tgcagccagt gcgatcattg ccgcagcact aacaggtgcc
     4981 tccagtcaag atgcacaaga cggattaaaa gaagctcgtg taccaggccg aatggatcaa
     5041 ttgatccaat ccaatggagc gcatgtctat gtggactatg cacataatta tctaagcttg
     5101 aaaacattgc tgaaatttgc caaaaatgaa catccagatg gccgtgtaat tgttgtatta
     5161 ggcagcccag gaaacaaagc aatctcccgt cgacatgatt ttggaaaagt gctatcagaa
     5221 acagctgaca tcgccttttt gacggcagat gacccagcat ttgaagatcc gaaaaaaatc
     5281 gcggaagaaa tcaatgaagc aattactaat ccagatttga tcgttcatta tgaaatggat
     5341 cgtcctgaag cgattcgacg cgctttagca gaaagcgctc cgcaagattc agtcgttatt
     5401 gcgggaaaag gcgtggatcc ctatcaaaaa atcaatggtg tagatgaacc ttatgaaggg
     5461 gattatgcaa tcgtgaagcg gttgattgaa gaataatggt atttatatac tagtggtttc
     5521 ccatttctaa gaaaaaaagg gactgtgatt ttttgtcaca gttccttttt tcttagaaat
     5581 cattttaact tttcactagc taattctgct gccttttctc tttcttcatt taataattta
     5641 ttatcataga atttgataaa tgggaaccag ataatgaatg cgatgattgc acaaatgact
     5701 gctaacacag cagctttcca atcaccacca ctaccaacaa atgctccgat cccgactggt
     5761 gaaggccacg gcatctgtgc aagcatcggt ttgacgattt ccagccgaat ggcaaaataa
     5821 gcgagtgtag cagaagccat cggagctaag aaaaacggaa ccgctaaata gggattgtag
     5881 acgattggca tcccgaaaat gattggttca ttgatattga ataatcctgg tacaagtgag
     5941 gcttttccta acactttcag ctgatcagat ttcgcgagga atgcaataaa gatgatcaat
     6001 cctaaagtcg cacctgatcc accaacggtt acataagaat tgttgaattc tcctgccaac
     6061 ggataatttg cgccttgtgc gttagctgcc atattagcta atacgatcgg ggtcaagaaa
     6121 gaagtgatga tattcgctcc atggatccct acgatccaca atgcatggat caagaaataa
     6181 atcaccatga caccaagcca tgtattcgtc aactgcgtaa caaacccaaa tgggatagca
     6241 atcattttga agatatccgt tcctagtgca actaataatc cattgatgat catcaccaca
     6301 aacgcaatga caaatgttgg gatcagagca gtgaaagaac gagaaactcc tggcggaaca
     6361 acatctggca tttttatgac ccaatttctc ttgacacaca tacaatacaa ttcgaccgca
     6421 ataatcgaca taataatagc tgtaaagatt cctgaagttc ctaaacgttc gacaaaagag
     6481 cccatcctaa taccatttac tacagtttct gtctcattga tagaagcaat caagctgatt
     6541 ttaccatcag tcatgatcaa ttcagggata cacataaaga atgcaaaaac agatagtaat
     6601 gcccccgtga gcggattaac atttaatttt tgttcatctc gttcaattga cgttaattca
     6661 taagcaaaca caatggcaaa gtaaatagat aaaatcccca tcgtcattgt attgacgatc
     6721 atgtacaagt cactaacttt gaaaaaagtt gcattaaaaa atccttctaa aaatgggaac
     6781 ggtaatggca aaatatttaa aaccaaaaac atcgagccta caatcgtgaa aggaatagaa
     6841 gccatccccg ctgccatgac agcccgaacg attttaagtt gcgcaatttt ccccataggt
     6901 cccatcaggt atttctgcaa aaaatcaaac attagttatt ctcctttcct aaatataacg
     6961 ctttttcata ttgtttaata cggatatact gttggtcttg gttgagtgaa attttcttta
     7021 aacgatactg tataaatgca ctggcaaaaa taccgcttac aacaagaatg aaaacaaata
     7081 actgtcctcc attgttattt gccataaaag gatacaagaa tgtaaatcct gaggtaaaca
     7141 acactggaat cactaataaa ttaaagacac tctggaacaa aaaataccac tttgtccatg
     7201 gaacgttcgt ttggtgtttt cgataaagct ttacttgttc tcctactgca ggcaaaatag
     7261 ctaaaatcag aatcatcggt aaactacttc ctaaactttt agctgataga agcagaatca
     7321 tccattgcat gttcatgaaa aagaaaaagg cagtcaggta acgtacgatt agataacgat
     7381 tgaaatacat gtttttcaaa ctaagttgct ttttttctaa ttcgattttc ctttttactg
     7441 tatcctgcac aatgatcctt cctttctatt tatttttttg atacaaataa ttcatttcct
     7501 gtgcaacttc caataatgtc atcgtcgtca tcagatgatc ttgtgcatgg accatgataa
     7561 tttccatttc tatttttgtc ccgctggcat attctttcag aagttgagtt tgtgactgat
     7621 gcgcatgaag aatctcttca tttgcttgtc gcaacttctc ttctacttct tcaaacatct
     7681 ctttacgcat cgagcgaaat gcttcatgta tcagtgtccg tgcatttccg ctatggaaaa
     7741 tgatatcaaa agaagtcact tgtagtgttt cactgtttat tttttcagcc atctgttttc
     7801 ctccttattc gatcagtttg ataaatttac tgctgaaatt ctcaaaaata ggttcagcta
     7861 agatttcctg ctgtactttt agctggtcaa ctaacctaac gattgcttta gtcacgaccg
     7921 taattccttc attttcgaca taagaaggcg aaactaaaaa aacaaaatga atttcttgat
     7981 attgatccca ctgcatccca gaaggaatca atgcaacggc tacctttgtt tcgattccaa
     8041 ctggaacagc gggatgtggg actgccactg tatcactgaa tatgatttgt cccatatttt
     8101 cacgttgctg gatctgcttt cgcatttctc ttatatatcc ttcttcttca tttatgctca
     8161 gcttttgcag caaatcatga agaatttctt cctttttcgg ttcgtacgta taagtcttga
     8221 aaaatttttc agacaactgt tcggtgtaaa tctgcttttt ttgctgtaga gaaaatgctg
     8281 gtatcggtct atctgaggaa tgatagggta tagattctcc aactaccttt cgtattttac
     8341 gaacatcatc ttcattcaaa aaaatactga catgtaaaac tggaattttg aaaaacattg
     8401 tcgaaagatc aatagaagaa ataatcaggt cgattccttt taaagttgtt tcattgatct
     8461 cataatagcc ttttacactt accactgtaa tatttttacc gaattcactg actacgcgat
     8521 tctttaatag ttgggcactg ccgtaacccg ttgcacagat aatcaatgca tgtagtttat
     8581 gcgcagctct ttctttttcc aatgccgcca tgagatgcaa agccaaatag gcccattcat
     8641 cttcattgat ggtatagttt ttcaaagccg gcatgttgct gaaatgcctt tttactgcgt
     8701 caaagacttt gggattttct ttctttatct cctcagtcaa tgggttctca atgcgaatac
     8761 ctcggctcaa tcgaatcatc atcggtgaca aatgatcgag tagtccattg aaaagctgga
     8821 gatcctctac taatgaaaaa tgaaattcct gagacaatct tttcagaact ttcgctaatt
     8881 cctttgatag ttcctgatca ttttgatttt ctttgtgatt cgacttagcc atgagatgta
     8941 atgtcaaata agcaatttct tcttcgggaa acgatacacc tgcgattacc tcaatacgtt
     9001 gaacgatccg ctgcgccact tgatactcta ctcgcttaga aacttcttgt tcgatgccta
     9061 attccttgat ttcaaaccct tctcggagcc gtttgatccc caaagccaag tgtaaagtca
     9121 gattctgtat gataaaatcc gaaactttta acttagcttc tcttgtttca tcgagaatga
     9181 taatgactag ctcttcaaaa ctgatgtctg ggaaaaaacg gctttgccct aaatattctt
     9241 tcaatgaatt tccataatgg tttccaaaaa aagtatccat gataaaatgc cgtcgatttc
     9301 tttcttcccc ttcgatccat gtcccttgac catgcttaga aacgattttt agtgaatatg
     9361 gaatcaatcg ctccttgatt tcttgcatgt cctttgtcaa actggaacga ctgatataaa
     9421 gctcttctgc caaatcatcc ataaaaagaa tggcatcttc caaaagcaat tggttcaata
     9481 tatacttttg tcgatcttcg gactcataaa atccttgcgg ttctctagca tatccaggta
     9541 ctatttcttg ccgactcaaa aacaagtcaa atgtcagtct ttgtaaaata tgcagttggt
     9601 agccataacc tggtttagca atgatcttac ctccgttttt ttctactaac tctttgatcc
     9661 gctgcaagta attacggatt gtccgatcag ataataacaa ttcagaagct aaacgttgac
     9721 tggtgacata ctccttcttg tgatcaatca aatatctcaa cagcaatttt tccttctgtt
     9781 tcattatcat ccactcctat tccatcatat gttctcttcg atcacctctg ctaacttctg
     9841 gatgcctgta gggattggga catacgcttg gaaagggata ctaacaacag gctttccaac
     9901 agattctcct aattttttga atttatccaa aaacatcgtc gtttgcgggc tgatcagaaa
     9961 taaatcaaaa tcactattct taatcatatt atcgccttct gtagctgaaa ctgcatctac
    10021 gagtacctct tctctttttt gcttaaagaa atccgttgtc tttgaggcca tcatcgagga
    10081 tgacattcct gccgcacaaa taatcaatgc ttttttcata aatcaatcac tccttttatt
    10141 tttataaacc aagcatatct tgtaagcgtt ttctattcaa aatatatatt tccgtagcat
    10201 tgcggaaaaa atgtgtatca tatcttgaat aataatttct ggcaccgtga tatactagtg
    10261 atagaatttt ttcaggaggg gatttagtga gtatcacagt gaatattttg tatacaagta
    10321 caaatggaca agccagaaaa tttgcagaag aaatggtcga aagaaacatt gtcaaagcga
    10381 ttcgtgcaga agaaggaaac gagaaatatg actattactt tccactagaa gatccagaat
    10441 ctcttttact gatcgaccgt tggaaagatg aagaagcaat cgaaaagcat cacaaatccg
    10501 aaatgatggc tcaaataaac gaactccgta aaaagtataa attaaaaatg aaagtcgaac
    10561 gtttcactga tttaccgtaa aaagatacac atggaatgat agcaatttat catccatgtg
    10621 tatcttcact ttttccttat tttttattcg atctcatcta atgattcaaa ccgtgttttt
    10681 ctcttatttt tcggataagt atttcagtca ccccaatata catgattcct aaacataccc
    10741 caaggatatt ctcaaaaatg cggatcgtta ctgctcctgg aaccccgaat aaactggcag
    10801 tgattgccaa agcaccaaaa gaattaaaaa ctgttttata tctataggta gaacaaacac
    10861 ctaaagctag accacctaat ataccgaccc agttaaaagg aataaattgg ctgataccaa
    10921 taaataaaac acatccaatc aatgagccaa tgattcggtc tactgctcgt tctttgaaac
    10981 caatcgacat caatccatag ctagatacaa tagaagaaaa tgcaaaccct gcccacatca
    11041 atcgctgaaa agggacatac tctcctgcaa cgatcaaaag actaatgcca aatgcataga
    11101 tgagcatcca aattttttcc tgcgaataca tgcctttcaa aaaaatctct ttaaacaagc
    11161 ttttgtcttt gttcttttct cgatgttttc tgtaaaggag aactgaaaac caacaaaaaa
    11221 acaaaaataa taagctactt gtttgtgtaa agaaattctt gttcaacgaa tctttaggta
    11281 acgaataaac aataaagaga tatgaaaagc cgtacaaaga agcattcccc attttgggat
    11341 cactagcggt tgccattaat atgctgctta gtaaaataaa atgaatcaac atgcccataa
    11401 ctgaccattt gatcagctgg acatacggtg ctactaatag tgagaacatg acgattgcta
    11461 aacctatgat cgaatgggaa atcttataac caaagtcaac aaaacgaata ctcatcaaca
    11521 tacagaataa aaccacagcg tatggtttat gcttcgattc aaataatgtt gtaattataa
    11581 tgataaaaca aatggcaaac aatacgatga ataatgatcg taaaaacaat gaacaaatat
    11641 acatgcactt ttctttttta gaagctgctt ctcgtatttt ttgttttaaa ataaacggat
    11701 ctagttgtag agactgataa aaatccacct gttgtcgtcc tttcttttta cttttatttt
    11761 ttacagtcat ctatgacagg ttgtaaatac tatggaacat catatggcgt tgtcattttc
    11821 ttgtcaacta tttttcctat tcatgttttt ataaaaaaac atatcgtttt cttgtatgat
    11881 taaatcacca caaaataaac aactgagaga acgatatgct ttatgaatgg ttctatcaaa
    11941 aaaattctga gatcaataga caaaaatttg atgattacag aggtctctta cgaaacctta
    12001 cagaagaaaa aaacattgat cgttgctgca gttctcttcc ctgctccatg tgtctgtaga
    12061 aattgtggtt ctgctgtggt agatggaaat aggaaattaa tggtggtgaa aaacaagaag
    12121 aaggaaacca ttgtccactt tgaataatat aatcatatgc caatggttat acgcctaaaa
    12181 aagcagcgct atacctgtaa aaactgtcga agccattgga acgctcaaag ttattttatc
    12241 cgtccaaggc actcgatttc aaatcatgtc agacataaaa tcacttcttt actgactgaa
    12301 aaagtgtcgt tattttttat ttcgaaaagt tgttaggtat ctttgacaac cgttattcgc
    12361 acattgaaag agttgagaag ctatttacca aagtcttcca aaagaattct gtcttgagtg
    12421 ttgagggttg tgaatttcgt tctcacgttt ccgttgagga taaactgagt tttatttccg
    12481 cggatggcga gacagaaaaa ttaatcgaga aaatttacct acacaaaaaa ttcctcgatt
    12541 aacaaactat ttccttagct gcactaatcc taaagaaata gaattcttag ttacagacat
    12601 gaacgccacc tactttcagc tcaccaaaca tgttctacca aatgcgaaat tggtgattga
    12661 tcgattactt ggtttttcca cgcctttcaa ggaggcgtaa cctttattca cgagttggtt
    12721 gaagctttcc gagacaaaag acccgacttg tttttctcct tattgacaaa gagccattaa
    12781 aactagagaa aaaattacaa ctgatcgctg attatttgta gaaagaaaat caaagtatta
    12841 cccaataaac ttgagtcatt aggcaaaaaa cactgcgaaa ctcaagaatg attttcgcag
    12901 tgtttttgta tattttttat taactagtat tactacaaag gtttattttt cacttctcaa
    12961 cgcagcacaa aattgtcttt acttatttac tggttgggtt caaccttctc tactccgata
    13021 ctaatatttg tttgtatacc ccttctaaaa tcggtagttt ccattcaatt gatgggtact
    13081 agttgatttt aaaaactatg cttattcaat tttttatttt agctcatata tttcgtttct
    13141 ttctaaattt tatactgatg aaaacaagta gcacacccaa tattgacaat ataaactggc
    13201 taatgatggt ttctccagtt tttggaagat gcttatttcc actgcttgtt ggattgtttc
    13261 ctcttatatc tgagctatca gcaacttttc tattctcttt tgttaaaaag atcgccactt
    13321 cttgttcttt ttcgatcttg aaagttactg gtgtcgcatc caattcatac cccgttggcg
    13381 cttgagtttc taccagctga tatgctcctg gtgctaagcc atctatcgcc agttttccat
    13441 ctgctcctgt ggttagacct gactgcagaa cggttccatt ggcatcttgt aactcaaaaa
    13501 cagctccttg caggacttct ccgctttggt catcgatctt tgtcaacacg acaccgcctg
    13561 gtgtaagtcg attttctttc gtcaattcta ccaccgctgt ctgacttctt tcgatctcaa
    13621 actcgattgg tgtcgcatcc aattcatacc ccgttggcgc ttgggtttct accagctgat
    13681 acactcctgg tgctaagccg tcaatcgcca gttttccatc tgctcctgtg gttagacctg
    13741 actgcagaac ggttccattg gcatcttgta actcaaaaac agctccttgc aggacttccc
    13801 cgctttggtc atcgatcttt gtcaacacga caccgcctgg tgtaagtcgg tttgctttcg
    13861 tcaattctac caccgctgtc tgacttcttt cgatctcaaa ctcgatcggt gtcgcatcta
    13921 attcataccc cgttggcgct tgagtttcta ccagctgata tgctcctggt gctaaaccgt
    13981 caattgccag ttttccatct tctcctgtag ttagaccggt ttgtagcgtt tctccttctc
    14041 ggttctgtaa ctcaaaaaca gctccttgca ggatttcccc gctttggtca tcgatctttg
    14101 tca
//
LOCUS       DAGCYL010000065        13934 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.066, whole genome shotgun sequence.
ACCESSION   DAGCYL010000065 DAGCYL010000000
VERSION     DAGCYL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.066"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            160..1278
                     /locus_tag="MB216_001439"
     CDS             160..1278
                     /locus_tag="MB216_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526281.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYYEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAIFRY"
     gene            1781..2101
                     /locus_tag="MB216_001440"
                     /pseudo
     CDS             1781..2101
                     /locus_tag="MB216_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2553..2753
                     /locus_tag="MB216_001441"
     CDS             2553..2753
                     /locus_tag="MB216_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4526282.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            2900..5689
                     /locus_tag="MB216_001442"
     CDS             2900..5689
                     /locus_tag="MB216_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBT4526283.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            5736..6263
                     /locus_tag="MB216_001443"
     CDS             5736..6263
                     /locus_tag="MB216_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBT4526284.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            6284..7474
                     /locus_tag="MB216_001444"
     CDS             6284..7474
                     /locus_tag="MB216_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBT4526285.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIETIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            7514..8812
                     /gene="asnS"
                     /locus_tag="MB216_001445"
     CDS             7514..8812
                     /gene="asnS"
                     /locus_tag="MB216_001445"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBT4526286.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            9865..10539
                     /locus_tag="MB216_001446"
     CDS             9865..10539
                     /locus_tag="MB216_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBT4526287.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            10536..10877
                     /locus_tag="MB216_001447"
     CDS             10536..10877
                     /locus_tag="MB216_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526288.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            10907..11266
                     /locus_tag="MB216_001448"
     CDS             10907..11266
                     /locus_tag="MB216_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBT4526289.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            11790..13871
                     /gene="cadA"
                     /locus_tag="MB216_001449"
     CDS             11790..13871
                     /gene="cadA"
                     /locus_tag="MB216_001449"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBT4526290.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         4644 a         2404 c         3011 g         3875 t
ORIGIN      
        1 taattgagga aattatgaag ataacttaaa aaaatagcta aatttaaaac cgtagaaacc
       61 gttttatttt tttagatacg ttttattgta tggaaaaata gtgttgtggt agactttaga
      121 tagaaacaaa ggaatgattt ttttaggagg ggtacgttta tggcaaaaaa ggataaagaa
      181 ttattgtcac aattgacttc agaaggcgct aaaggaccat tcgtcacgat catgctgaat
      241 acgcatgtcg cccatcaaga tgtggaaaaa gatcagatca aattcaaaaa ttttgcgaaa
      301 gaagcaaaga aacgttttga gaaaaaatat tacgaacatg actggtcccc gttccaagaa
      361 aaaattgaaa atttgttagg agatccttcg ttttggcgta gtgcaactac gagtgtagcg
      421 gtcattataa ctgctgacca aacctatatc catcgtttga gtatcccggt agacgatcaa
      481 tactatgtaa gtgatcttcc ttatttactg gctattatca aaaatgggca gttcaactat
      541 tcttattatt tattgggatt gaaccgcgac tccatgaaat tgtatgaagt cagcaacaaa
      601 caagtaaaag aagtatctct accagaaggg gcaccggttg atgtcgttac tgcattggga
      661 tctgaactga caggtggcgg tttgaacttc tcttctaatg gaggaggcgg agcccgagat
      721 ggagtaactt tccatggcgt aaatccaaaa gatgaagaag tagagatcga ttgggtcaac
      781 tattaccaag cagttgatac gtacttgaag gattctttcg aaaatccaga acaatggccg
      841 atttatttat ttgctctacc agaaaatcag acgatgttca aaaaaattgc taaaaatccc
      901 tattattgtg cagacgttgc gatttctgct tctcctgcgc agctatcctt tagagatatc
      961 gaagaagggg cgaagaagct gtcaagtgaa ctcgcagctc aagaatcaaa agaatacaat
     1021 aaactcttag ataagaaatt catcgatcag ttagttgata tcgttccagc tgcgaaagaa
     1081 ggaaaagtgt cacatttatt tatcgctacg tcaaacttag ctgatggttt cggtgaagat
     1141 cctgaaacag aatatgatcg gcgtcaagtg ttgaatgcat tagcagatga tgtgatcatt
     1201 aatggcggaa aagtgtttat tttggatcaa aaagatgcgc cagatgaaaa gagtctgaca
     1261 gcgatttttc gttattaaaa gaggttgtga aatcagtgtt ttgacctgag tattagagaa
     1321 acaaacggaa aaatagtttc acatattttg ccgttttttg agctaatacc gaaggtcagc
     1381 tgattaaaca ccgtgtatga gaggttgtga aaaagctgtt ctgcatcaag taataagaac
     1441 agcaagcaaa aatagctctt catatttttt gtttggtggg cttattaccg cagatgcaag
     1501 cttttgaaca ccgtttaagg aagaggttgt gacagaagtg gtcagcttca agaaataaga
     1561 agagattgac aaaaattatt tctcaaattt ttgtgaaatt tcggcttatt tccgaagaag
     1621 ctacttctgg aacaccgttt aaaaaagagg ttgtgacgtg gtcacgacct ctttttgacg
     1681 attatgatac aaaaaacatc atttatgaac aaaatgtgaa taacgggcgt tcttgagata
     1741 tgattttaat tgggaaaaac ttatcgataa ggaagtcttt atgaaaaaaa gactgttatg
     1801 gtgtttgctg tttctatccc taagtttaac aagtcagcca gtttttgctg aagaaacaca
     1861 gcggtctcct attacaggca cacgtctgta caaaaatgtt tatgtacaga caccaaatct
     1921 tgattctgcg attcctgaaa cagaagaacg gtctttgcag ccaagatttg gttacttttg
     1981 gtactgtccg acctgtgatt aagaaagttg ctggcatttc ttacattcta cagctgcagc
     2041 ttttgctttt actcatactc agaaatatga tggacatcat cctgttattt atgaggtata
     2101 gatatgagca aaaaagaaaa actaatctcg ttgatcgtat tggtttgcct tgttcttctg
     2161 gtcactgtca taggctatca gaaataccat gagaacatca gacctactaa agaatcttcc
     2221 ccagcctcga ccgttgaaac agaaccgata agtagtcaga aagaataatt tttttagttt
     2281 aaagaagaac atcagagcaa aaaaacacca actgaacaat atgtcgttta ttttaaaaaa
     2341 agttcggttc ttcactttat gtaatcgctg agaaaacttt ttttaataaa aaaagtcgaa
     2401 aaactctagc ttttttgtaa acggtatgat atactatagg agtagttttt gttcggtaat
     2461 ctgtggtagc ttgtttcaaa taaacatctt ccagatatac accattcaag taattgcaaa
     2521 atatgtgttg atacacaagg aggattttta ttatggaaac aggtacagta aaatggttta
     2581 actcagaaaa aggatttgga ttcatcactg cagaaaacgg aaacgatgta ttcgttcatt
     2641 tctcagctat ccaaggcgac ggattcaaaa ctttagaaga aggccaagca gtaactttcg
     2701 atgttgaaga aggccaacgc ggacctcaag caacaaacgt tactaaagct taattaacct
     2761 atactaaaaa ataaacgccc agacatttgc gtgtctgggc gtttttgtct attttcttta
     2821 aaaagcgatg agagtatttt tttgcacttt gtagtataat ttcaaaagaa cattagaggc
     2881 aggagaagag gaagatattt tgaaaccaag aacttatgca gtcgttgata tcgaaacgac
     2941 tgggacgaat ccaaaagaag atcggatcat ccaatttggc tgcgtcatga ttgaatcagg
     3001 tcggatcact tcacgttttt cgatcgatat tcatccaggg cggaagattt caaaacaaat
     3061 ccagcattta acagggatca cgaatcaacg cgtccaaaaa gcgccttatt tcgaagatgt
     3121 cgcccaaacg atttacaatc ttttggcaga caccatattt gtcgcgcata atatttattt
     3181 tgattataat tttttgaacc atgaactcat gcgatgcgga cttcctagtt tgaagatacc
     3241 aggcatcgat acagtagaac ttgcacaaat tttcttgccg acggaaccaa gctttcgctt
     3301 agctgatttg tcagaaagcc ttggatttat tcatgagaat cctcatcagg cagatagtga
     3361 tgccgaagtg acaggtcaac ttcttttgct gatcgaagaa cggatgagaa aattgccaat
     3421 catcacgtta gagcagattg cgcgattaag tagacacaca ggtatggata cgagtcgttt
     3481 catttatcat gtgattgaag aaatgaaaga aaaaccagag ccacttgatc cctcattaga
     3541 aatcgtcgat gggctagctt tgcagaaaaa agaagtggaa ctattcacat ccgtccatta
     3601 tggcgagcgg acatatccaa gaaaaaaaca agcaaaagaa aaaatgtttg gtaaaacact
     3661 gatgtatcga aaagaacaga acagactgat gaatgcggtc tacgaccatt tcacaaaaga
     3721 tgaatcaaaa gatttgatga tcgaagcagc tacaggaatg ggtaaaacaa tcggttatct
     3781 gctgccattg agttatttgg ctacacctga aaaacccgct gtcatcagta ccgtttcgct
     3841 agttttgcag cagcagatca tagaaaaaga cattccattg ctgaatcaat tgctagatca
     3901 gcccatacaa cctgtaatta tcaaaagcta tcgccactat attgatttgc agcgttttaa
     3961 aggcacactt gtggaaccgc cagaacaaaa acaatatgct ttgtatcaaa tggcggtctt
     4021 agtttggctg acgcaaacga agactggtga tttggatgaa ttacatttaa caaatttgaa
     4081 ccattcattc tttgcagata tcgcgcacag aggtattgga tttttagcaa gaaatcagtc
     4141 attttacgaa caagactttg ttcgtttctt gcaaaagaaa atcaggcaaa gtaatttttt
     4201 gattgtcaat catgcgtttt tagtacaaga aacacagcga aaagaaccat tgcttccagc
     4261 tagtccattt ttgttgatcg atgaagtaca tcatttgccg gaaatcgctg aaaaagtaaa
     4321 tgacaaacga ttaagtatta actatttcca taaacaaatc cagcaattag aagaaccaaa
     4381 tcagctgttt gaccgaatac aaagctattt accgaaagaa catgaagcct tacgctggtt
     4441 atcgatttat cgagaagaac tttctgcgct atgcgaagca cagatttgta tggcagaagg
     4501 attaatgagt ttagtgagcg gcaagcaacc taacggacag tatccagaag aagtcatgat
     4561 gacaaaagag atgttggatc agctgccact ggaatctggg acagccattg atcacctgca
     4621 gcttttttat caggagatcc agcagctaca gtcacaaatg cgtctgactt ttgagattat
     4681 ccaagaaagc tggacaaacc agatgcggat cgatttagca agtttatata gcctatttga
     4741 tcaattagac aagcaatact tgctgatgga tcaatggctg gaagactggc gagatcattt
     4801 gatccattgg ttcgttcctg ggaacacagc gcagcagatg atttttcatc tccatgattt
     4861 tcaagcggct atgttgccgt cgactgtttg gtatcctcga tatgatcgta ttctttatat
     4921 tggaggaacg ctgaagatcg gccctaatcg acactatctc tctaagaggc ttggcattga
     4981 agcagcacct cttaaagtga ttgcctctcc ttatgattat gagaaacagg caaggatcta
     5041 tgtgccaaaa gaggcacctg ccgtccatca agtcagcaat caagcatata tcgattattt
     5101 agagacagca cttacaggtc tgatcgaaca agaagatcgg ccaatacttg tgttattcac
     5161 ttctcatgat gttttgcaga aggtctatca gaaaatgcat ctgtctatgc tagaaaaagg
     5221 acgagaaata ctagctcaag ggtttggtgg aagccgggag aagcttttga aacgcttcat
     5281 tttgtcagaa aaaagcgtat tgtttggcgc tgacagcttt tgggaaggtg tagatttgcc
     5341 tggagatgct ttgcagatcg ttgtagtcac acgtttaccc ttcgataatc cgcttcgccc
     5401 gatggtcaaa gctaggaacg cttatttaga atcagagggg atcaatcctt tttaccagga
     5461 atccgtacct aaagcagctt tgaagttacg ccaagcatta ggtcgcttga taaggacgga
     5521 gacagatcgt ggcgtattgc ttattttgga tcgaagacta gtcactgcaa aatatggcgc
     5581 acggatcatc catgcattac cgaaaaaagt accgatcaaa gagctagcta tggaggaaat
     5641 ctatcaggat atccatgaat tcttaaagaa aaatgaagaa ggaagttaga ggagctgtct
     5701 tttttcataa ttaaaatctg ctataatgaa aaggaatgaa gataaaggag cgacgaccct
     5761 tgaagaaaaa acagacaaag gaaaaaacga ttaatcgcgt gttacttggc ttgatcgtat
     5821 ttttattagc ggtcatcgtg atcagctcga ttttttactt gcgttccagt cgtccggcgg
     5881 tgcaggcgaa gaaagaagca gtagaaatcg caaagaaata tgcaaatctt gaggaaaccg
     5941 atcattttta ttggtttact cgaaagaaga catacttttc actgacaggt acaaatgata
     6001 aaggaaaaga aatcgccgtt atcattccta aatctggtga aaaagtaaaa gtattggatc
     6061 aaacaaaggg actgactgaa aacgaagcaa aacagacaat tgctgcggcc catcctgaaa
     6121 tccaaatcga aaaagctgct ttagggatgt atgatgatca gcctgtgtgg gaagtagtag
     6181 gaaaagaaac aaatgggaaa ctgaattatt acctgattgg ctttgaatct ggagatgaag
     6241 tcaagacaat caagggaatt taaaaggtaa aggaatgaat gggatggagt tatcaaaaag
     6301 aacaaaacga ttagaaccgt ctgtcacttt ggcagctgcg gcaaaggcca aggcattaaa
     6361 agcaaaaggc agagatgtgc tgagtttgac tgtgggagaa ccggattttg cgacaccaga
     6421 aaacatccaa gaagcagcaa tcgaaacaat ccgcaatggg aaagctagct attatacacc
     6481 aacagcagga atcccagagc ttcgacaggc aatcgttgat tatctgaaga aaaactatgg
     6541 acttgtctat gaaccttcac aaacagtcgt aacagacgga gcgaaatttg ctttgtatac
     6601 ccttttccaa acgatccttg acccgcaaga tgaagtcatc attcctgtgc cttactgggt
     6661 cagttacggt gagcaagtaa aactggcaga aggacttcct gtctttgtga aaggggaaga
     6721 atccaatagc tggaaagtta ctgttgaaca gctagaacaa gcacgttcag aaaaaacaaa
     6781 agccgtaatc atcaattctc catcgaatcc gactggaatg atttatagca agaatgaact
     6841 gcaggcaatc ggtgaatggg cagtaaaaaa tgatattttg atagttgctg atgatattta
     6901 tggtcgttta gtttataatg gcaatgagtt tactccaatt gcaacgattt cagaagcaat
     6961 caaaaatcag actatcatca tcaacggcgt gtctaaaaca tatgcgatga caggctggcg
     7021 cattggttat gcggtgggaa atcctgtgat catcaatgga atgatcgctg ttgcttccca
     7081 gtcaaccagt aatccgacag cagtcagtca atatgcagct gttgaagcat tgaaaggcga
     7141 gcaagataca gtggaagaaa tgcggaacgc ctttgaagaa cgcctgaata cgttatattc
     7201 gcttgtggca gaactgccgg gtgtttctct tgaaaagcca caaggagctt tttatctttt
     7261 tcctaatgta aaagaaacgc tacgcatgtg taaatatgaa aatgtcacac aatgggtaga
     7321 agatctttta gaagaaacag gggtagcgct tgtgactggc gaaggctttg gggcaccaga
     7381 aaatgtccgc atgagttatg ctactgatcg cgctacgtta gaagaagcgg tacgcagaat
     7441 caagcaattt attgagagta aaagtcaaaa ttaatcctaa agaaattcaa ggaatataaa
     7501 aggagagtca tacgtggaac aaattcaaat catcgattcg aaaaaccatg tgggagaaac
     7561 agtaacaatc ggcgcttggg tcgcaaataa gcgatcaagc ggaaaaatcg catttttaca
     7621 attaagagac ggaacagcct ttttccaagg tgtagttgta aaaagcgaag tgtcggaaga
     7681 agttttccaa acagctaaaa atctacacca agaaacatcc gtgttagtaa ctggagaaat
     7741 ccgtgaagac agtcgttcaa aatttggtta tgaaatcgga attacaaatg tggaagtggt
     7801 aagcgagagc cacgattatc cgatcacacc aaaagaacac ggaacagact tcttgatgga
     7861 tcatcgtcat ttatggctgc gttcttcaag acaacatgcg attatgcaga tcagaaatga
     7921 aatcattcgt gcaacgtatg aattctttaa tcaacatcat tttgtcaaaa ttgatccgcc
     7981 gatcttgact ggttctgcac cagaaggcac tacggatcta ttcgaaacaa attactttga
     8041 tcaaaaagct tatttatctc aaagtggcca attatacatg gaagctgctg ctatggcttt
     8101 cggaaaagtc ttttcattcg gtccgacttt ccgtgctgaa aaatcaaaaa cacgacgtca
     8161 tttgatcgaa ttctggatga tcgaaccaga aatggctttc actcatcaag aagaaagttt
     8221 agaagtacag gaacaatatg tagcattctt agtacaaaat gttttagatc attgcgaata
     8281 tgcattagat gtactaggac gagacaaaga agtcttaaaa acttatacaa aattgccatt
     8341 ccctcgtatt acttatgatg atgcgatcga attactgaag aaaaatggat ttgatgacat
     8401 cgaatggggc gaagactttg gttcacctca tgaaacgttc attgcgaatt cattcgaaca
     8461 gcctgtcttt atcctaaact atccaaaagc catcaagcca ttctacatga aaccacatcc
     8521 aacaagagaa gacgtggtga tctgtgcaga tatgatcgca ccagaaggtt acggtgaaat
     8581 tattggcgga agcgaacggg caacagacta tgatttcttg ctagaacaaa tccgtaatca
     8641 cggtttggat gaaaaagaat attcatggta tcttgatcta cgtaaatacg gttctgttcc
     8701 gcactctggt tttggtctag gtcttgaacg aacagtcact tggattgctg gtatcgacca
     8761 tgtacgtgaa gctattccat tcccacgttt gttgaaccga atttatccat aagaggtgat
     8821 gaaaaagctg ttctgcatca agtaattaga acagccaagc aaaaacagct gttcatgttt
     8881 ttgaattggc gggacttatt accgcagatg taagcttttg aataccgttt agtggagagg
     8941 ttgtgaaaaa gctgtttagt ttcgagtatt aagaaaaatt ttgtggaaat ggctttccac
     9001 atttttgcat aaatttggct taataccgag aaactggctt ttgaacaccg tttaaggaag
     9061 aggttgtgcg cctgccgttc agctccgagt cacaaggagt aatttcaaaa aacagttttt
     9121 catgtttgat gactattgtg acttgtgacc gaggagttgg ccggagagca ccgtttattc
     9181 taaaaaagaa ctgcgaactc tcgcagttct ttttttaaaa tacgggataa aggaagtttc
     9241 cagcagacaa gagaagaaag caaaagaaat tgctatttga ttcacaaggt tctataacga
     9301 aatcgcaatc aatcaaacat ttacaaagaa ggtaattcaa atggaaatct gatttgatga
     9361 tgcttctgct gttgaaataa agcagcatct agcacctgaa aacaaactaa tagtaacatt
     9421 cgaagatgga gtaggtccgt attttcagca tgcaataatc catatgtaag ttcaattcac
     9481 ccttgaaatc atcggaccag ataaagttat gaccaatcta tcccaagcaa tctagaagag
     9541 atattagtaa agggacattt actagaagtc ttagatgagc ctataaatat atgttctgat
     9601 ggcgtcatca gtggtctgat tgacggaagc ttaggtgtga tcgatctctc tggaccaaac
     9661 aacgaaggaa tcaaggggaa tccggcacgt taagaaaaaa atagcataaa aaactagaca
     9721 cttgatgagc gttcattcaa ttgtttgaag ggactaggct ggtgtcattt tttatacata
     9781 tattttctct ttcgtcttga tagggcttat gggataatat aaggaagaaa atcatcgttt
     9841 tattcaaaaa ggagtttttg acaaatgaaa aaacgtatat tatttatcgg tgatagcatt
     9901 acagacgcag gaagagaccg agcagaagca actgatctag gtaaaggtta tccacttttg
     9961 attgccggaa aactaatctc ccaatatcca acgatggaat ttgaatgcat gaaccgtggg
    10021 ataggaggag acaaaattgc agatctgcaa gtacgctggc aagaagattg tttagcttta
    10081 caacctgact atgtatcgat tttgatcgga atcaatgata cctggcataa tactagcgat
    10141 caggctatat tcggaactga gaaatctgct cagcaatttg aatccgttta tcgaaaatta
    10201 ctaaatgaac ttcaaacagt gggaatctgc aacatcatct tgatggagcc gtttgtactt
    10261 cctataaccg acgaccgtca tggctggcgt aaagacttag accctaaaat ccaaattgtt
    10321 cgtaaacttg ctaaggaata tggcgcattg ctggttcctt tagatggtat attcaatgct
    10381 ttagggattg tttatggttt ttcgaaatat acaagagaag atggggtcca cccaacgatt
    10441 ggcggacatg agatcattgc acaaagctgg atagatgcaa ttgccgcagt cgattttttt
    10501 agtggagata aaaatgagcg aaagaaaagt gagaaatgat ggaaatacgg tgggcagtaa
    10561 aagaagatct acctcaaatc aatcagctga ttactgatca tccagaatca ttcgtaaaag
    10621 caaaaacggc agctgaccta aatgataaga ataaactatt gctcgttgct gtaacaaaag
    10681 aaaaacggat tatcggattt gcgcaagcgg acgtgcaaca gtcagaagaa cgattggatc
    10741 agcgaccatc aggaaaacta atccaacaca cattagtaac aaaagaaaat acggaagaaa
    10801 tccaaaaaaa atttgcagaa tatttgatac tggaagctaa aaaaagaaga ataaaatcat
    10861 tttttgccca gttttaatca aaaacagtaa tagaaggaga aatacaatga gttgtttagg
    10921 aaatgtcatc tggtttatat tcggcggact aatcggtggt cttagctggt tagcagcggg
    10981 gatactttgg tgtatcacga tcataggtat cccaatcggt atgcaatgtt ttaagatcgc
    11041 ctccctttct tttgcgcctt ttggcaaaga agtaatttat caccagtctg gcatgtcatt
    11101 gattgttaat atcatctggc tgatcgtttc aggcctacca attgctctcg cacatgtcgt
    11161 cagcggcatc cttctatgta tcacgatcat agggatacct tttgccaagc aatctttcaa
    11221 acttgctcga ttggcgttga tgccattcgg tgcagaaatt gtataaaaga gaataataaa
    11281 ggttagataa agcatagagt caggtgggat acatgtattt cacctgactc tatgcgttct
    11341 cctgtataag ttcttccatg tgacttttac gtcttttatt tatatggggt tactgtttat
    11401 ggaaagttga aataattttc attaaagaac tctttctata tttttgaaag cgtagtggag
    11461 cttcgaatat ctgggaagga cattttgcag ggattgaaaa aagatattgt cctatcgaaa
    11521 aattaatgaa tttagacaaa ttgccagcaa caggcttcaa acttagttgc tatcctgtca
    11581 agatcaagaa ggcaagtgcg ggttggataa gagcagtggc aatgatcgaa aagaaaaaga
    11641 aagaataagc caaaacatct gtatggaaga aattttccga tgcagatgtt tttcattttt
    11701 tctttcggat accttgacat atgaataacc gttcatttat aattagttat gaagagatat
    11761 tcatatgttg aggcgaggag gtcattaaaa tgaaaagtta cagattagaa ggtttagatt
    11821 gtgcgaactg tgcaatgaaa atcgaaaaag gagttcagaa gatcaatgga gtcaaggaag
    11881 caacggtaaa cttcacttct ggaaaactaa cgattgacgc agaggaagat catttggcta
    11941 cgattgaaca agaaataaaa aaagtagtga aggaattaga accagatgtc aaagtaacag
    12001 aaatagataa ggaaaaagta tctgaacatg gaaatgaaaa agagagaaat acgctgttcc
    12061 ggattttatt ttcccttgca ggtatcgctt tattgctatt atttgatttt aatgaaccaa
    12121 ttcgcttgat agggtattta ttgatttatc tgctgatagg atatgatgta gtgaagaagg
    12181 cagtgatgaa tatcgtcaaa ggaaaaatct ttgatgagaa ttttcttatg tctgttgcta
    12241 cgattggcgc tatgttgatc ggtgaatatc cagaagccgt agcagtgatg ctattttatc
    12301 aaatcggcga gtatttccaa ggattggcag tcagtcattc aagaaaatcg attcgcgaat
    12361 tgatggcgat ccgtccagaa acagctcacg tgcagacgac ggaaggattg atgaccgtga
    12421 accctgaaga cgtgcagatc ggtcagtttg tacttgtgaa gcctggtgaa cgtgtaccgt
    12481 tagatggaac gatcatcgaa ggagaatcat tagtcgatac gtcagcactt acaggagaat
    12541 cagtccctaa atctgtatat gttggtgaaa cagtattaag tggatttatc aataaaaata
    12601 aaccattttt agttcaagta gaaaaatctt atgaaaattc tactatcagc aaattattag
    12661 aattagtaga aaatgccagc agtaaaaaag caccagctga aaatttcatt actaaattcg
    12721 cgcgctatta cacaccagtc gttgtaggat tggccgtttt attagctgtt ttacctccac
    12781 ttgttgtttc tggagcggcg ttttctgaat ggatctatcg agcattgaca tttttagtga
    12841 tctcatgtcc atgtgcatta gtgatctccg taccccttag tttctttggc ggtatcggag
    12901 gagcaagtaa aatcggtgta ttagtaaaag gttcaaatta cttggagttg cttgcacaaa
    12961 ctgagacagt ggtattcgat aaaaccggca cattaacgaa aggtgacttt tcgatccaga
    13021 cgatcgatac aaaaatcgat ccaaagatat tcatgcaata tgttgcgagt gcagaacagt
    13081 tttctacaca tccgattgct caatctgttt tagaaggcta cgcaggtccg cttttaccaa
    13141 cagctaatat ccaagaattt gcaggtgaag ggatcttagc tgaagttgat ggcaaacaag
    13201 tattagcagg taatcacaaa ttgatggaaa gatttgaaat ctcttttcct tcaagtcaag
    13261 aaatcggtac attgctttat ctagcgatcg atcaaagtta cagtggttat cttgttattg
    13321 cagatacctt gaaagaagat gccgtagacg cattggttca attaaaaaag gctggtgtga
    13381 agaacacagt catgttgacg ggcgattcga aaaaaattgc tgatcatatc gggaagcaag
    13441 taggcgtgga caagatatat agcgaattgc ttccagaaga taaagtgcag cggttagagg
    13501 aaatcttaca aagaaacaat aaaaagaccg catttgtcgg agatggaatc aacgatgcac
    13561 cggtgctagc aagagcagat gtaggaatcg caatgggtgg attgggaagc gatgctgcaa
    13621 ttgaagcagc agatgtagtc atcatgaatg atcaaccatc gaagattgca gaagcaatcc
    13681 atttagcaaa gaaaacattg aaaatcgtca aacagaacat tgtctttgct attgggataa
    13741 aaattttggt acttgcctta ggtgcttttg gttttgcatc aatgggagat gcagtatttg
    13801 cagatgttgg ggtgacagta ttagctgttt tgaatgccat gcgaagcttg catgtaaaaa
    13861 ataaacgttg atcaatgaag aggacgaatg aacgtcctct tttttttata tccttttctt
    13921 aatgaaaaat gtaa
//
LOCUS       DAGCYL010000066        13816 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.067, whole genome shotgun sequence.
ACCESSION   DAGCYL010000066 DAGCYL010000000
VERSION     DAGCYL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.067"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="MB216_001450"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="MB216_001450"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBT4526291.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="MB216_001451"
     CDS             complement(2260..2511)
                     /locus_tag="MB216_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBT4526292.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="MB216_001452"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="MB216_001452"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBT4526293.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="MB216_001453"
     CDS             3461..3679
                     /locus_tag="MB216_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526294.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="MB216_001454"
     CDS             complement(3968..4480)
                     /locus_tag="MB216_001454"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBT4526295.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="MB216_001455"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="MB216_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBT4526296.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="MB216_001456"
     CDS             complement(6933..7376)
                     /locus_tag="MB216_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBT4526297.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="MB216_001457"
     CDS             complement(7377..8165)
                     /locus_tag="MB216_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4526298.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="MB216_001458"
     CDS             complement(8162..9103)
                     /locus_tag="MB216_001458"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBT4526299.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="MB216_001459"
     CDS             complement(9280..9867)
                     /locus_tag="MB216_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBT4526300.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="MB216_001460"
     CDS             complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="MB216_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBT4526301.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11698..12708
                     /gene="gap"
                     /locus_tag="MB216_001461"
     CDS             11698..12708
                     /gene="gap"
                     /locus_tag="MB216_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBT4526302.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12793..13728)
                     /locus_tag="MB216_001462"
     CDS             complement(12793..13728)
                     /locus_tag="MB216_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4526303.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3810 a         3095 c         2436 g         4475 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgtaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgtattaat tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg
    11701 acagtaaaag taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc
    11761 aaagaagtct cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg
    11821 ttagcacaat tattgcaatt tgactctact catggtactt atcctggcac agttactgca
    11881 acagaagact caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca
    11941 agcaaaatcc cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt
    12001 tatacatctg aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt
    12061 tctgccccag ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat
    12121 gaaaacgaca aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct
    12181 tatttcttaa ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac
    12241 acttccacac aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt
    12301 tcagctgctg acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt
    12361 atccctgagc tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt
    12421 tcattaactg aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa
    12481 gcaatcaaaa aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt
    12541 tctagcgatg tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta
    12601 acaactgctg gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga
    12661 tttacttgcc aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca
    12721 atcgataaaa gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac
    12781 ttttaaactc gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt
    12841 ttcaggatct gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt
    12901 tggtcccttc gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc
    12961 gtctccatgt ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc
    13021 ttcaccgaaa aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg
    13081 aaatgctgga agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga
    13141 ataaccgcaa tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat
    13201 cataactgtt gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt
    13261 gttttctagc actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc
    13321 aatgtaatcc ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc
    13381 catatcatga aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg
    13441 agccacaaga gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt
    13501 cagaccgtct tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt
    13561 atgtgcatcc gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc
    13621 tttctcagaa ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac
    13681 aatcagagct aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc
    13741 tcatttctct tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa
    13801 tgttcatttt tataac
//
LOCUS       DAGCYL010000067        13786 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.068, whole genome shotgun sequence.
ACCESSION   DAGCYL010000067 DAGCYL010000000
VERSION     DAGCYL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.068"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            260..598
                     /locus_tag="MB216_001463"
     CDS             260..598
                     /locus_tag="MB216_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526304.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(803..1042)
                     /locus_tag="MB216_001464"
     CDS             complement(803..1042)
                     /locus_tag="MB216_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBT4526305.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1018..1365)
                     /locus_tag="MB216_001465"
     CDS             complement(1018..1365)
                     /locus_tag="MB216_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBT4526306.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2097..2678)
                     /locus_tag="MB216_001466"
     CDS             complement(2097..2678)
                     /locus_tag="MB216_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4526307.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2861..3487)
                     /locus_tag="MB216_001467"
     CDS             complement(2861..3487)
                     /locus_tag="MB216_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4526308.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3509..4840)
                     /locus_tag="MB216_001468"
     CDS             complement(3509..4840)
                     /locus_tag="MB216_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBT4526309.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4995..5396)
                     /locus_tag="MB216_001469"
     CDS             complement(4995..5396)
                     /locus_tag="MB216_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBT4526310.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5482..5688)
                     /locus_tag="MB216_001470"
     CDS             complement(5482..5688)
                     /locus_tag="MB216_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526311.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5783..7285)
                     /locus_tag="MB216_001471"
     CDS             complement(5783..7285)
                     /locus_tag="MB216_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBT4526312.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7303..7758)
                     /gene="argR"
                     /locus_tag="MB216_001472"
     CDS             complement(7303..7758)
                     /gene="argR"
                     /locus_tag="MB216_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBT4526313.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7856..8473)
                     /locus_tag="MB216_001473"
     CDS             complement(7856..8473)
                     /locus_tag="MB216_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBT4526314.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8694..10067
                     /locus_tag="MB216_001474"
     CDS             8694..10067
                     /locus_tag="MB216_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4526315.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10142..11254
                     /locus_tag="MB216_001475"
     CDS             10142..11254
                     /locus_tag="MB216_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBT4526316.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11326..12963)
                     /locus_tag="MB216_001476"
     CDS             complement(11326..12963)
                     /locus_tag="MB216_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBT4526317.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13131..13739)
                     /locus_tag="MB216_001477"
     CDS             complement(13131..13739)
                     /locus_tag="MB216_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4526318.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3726 a         2897 c         2462 g         4701 t
ORIGIN      
        1 ctatataaca ctaagtaacg caaaattccc ctttgtaaac tatctagaaa attgttttgt
       61 ttaaagtata tcttattgga tataaataga aataaattgt agccagttca ttttttcttt
      121 aactaaaaaa ataatccaag aaacattgaa cctatggatc caaaagctac ccaaatacta
      181 tagctaaccc cccgctacct tcaattttgc agataaaacg ctttgggaga aaatttcaaa
      241 gacaacaggc catcctatca tgcccgatta taaacaaaaa gctggctatt ccttttgctc
      301 tgacaaattt caattgggaa agttcacgaa actagcaggt gaagaagttt caactgttcg
      361 gatcaaagaa tgcccgcttc aaatagaaac attggttcaa cgaatttcaa ctagacaaga
      421 ctttgctatt gtagaacgtg agatcgtagc tatttttgta gaagaaggga ttttacatga
      481 tccaactcat atcaatgtgg ataaatggca acctcttatc tataaattca gagaatatac
      541 aactgccaac cagtcgttaa ctttgaattt tagattccaa gaattcaaag agtcataatg
      601 ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac gagtaaaaaa caaatagaag
      661 ctatacatta gaaaggtcga ctgtgaccct atagatgcag tatatgcagc ttatgagcat
      721 ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc tttttcgttt tttgccgctc
      781 tttctttgaa aaatcgatta tgttatacct tctccattac taattcaggc atctgctgag
      841 taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg ttcgatttgt tcttcagtca
      901 agacaagcgg cattcggtca tggattggtg cgactgtgcg gttaggttca gtagtcatta
      961 tgattgattc tgccattttt tcatcctgat ggctacgata aaaaccgcca atatacacta
     1021 ttttttcctt atttgaaaac ataaatttgt ctttttcttc attccactca ttgggaaaac
     1081 acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg tctctgcacg
     1141 ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca tgatttccgg
     1201 agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg gaaaaacttc
     1261 tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc gataataatt
     1321 ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa tccctcccaa
     1381 tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc tttcaatttt
     1441 tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta ttctcctgcc
     1501 aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt ttgaaattgt
     1561 tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg tgttcaaaag
     1621 ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagctatt ttcacaaaat
     1681 ttttcttaat actcgaaact aagcgacttt ttcacaacct cttccttaaa cggtgttcaa
     1741 aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc tatttttatt
     1801 tggctgttct tattacttga tgcagggcag ctttttcaca acctctccac taaacggtgt
     1861 tcaatcagct aaccttcggt attagctcaa aaaacggcaa aatatgagga actattttgc
     1921 cgtttgtttc tctaatactc aggtcaaagc gctgatttca caacctctaa tacacggtgt
     1981 tccagaagta gcttcttcga aaaataagcc gaaatttcac aaaaatttga gaagcaattt
     2041 tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc taattattaa
     2101 agaacctttt gcgaagttaa caatctttct ttgatcaatt gaaaattctg cctaagtagt
     2161 gatagatcct gtgtgacttt caacaataag atacttccct ggatttcagc aatcaacgct
     2221 ttggcttgac ggtctgcttc ttcatcggac agtttttctt cagaaagtgc caggatattt
     2281 tgtttcgtat acttgaccca ttgattgact aattctagta gcaacagacg gtattcttca
     2341 tccactgtac tcaactcaat aatcaaatta cctacaggac agccatgata agtgatctgt
     2401 gtctcatgat cagcgaaaat ccagtccaac atttcttcta atgcttcttc tggctgtttt
     2461 ccagcattta agatgtcttc gaacaattct gtctgccata cgtccatatg cgcatggatc
     2521 accgctagac aaacttcttt ttttgaagaa aagtaataat aaaattgacc ttttcctgta
     2581 tcagaagctg ctagaatatc tcggattgac gtttcatgat agcctttttt ataaataaga
     2641 tctttggtcg cttccaagat cttttccttt gttttcaatg atattctcct ttttcatttc
     2701 tcatagtcct atattataca gtaaattcat cattttttct tcttaatcgt ttaaaaaaga
     2761 ctgtgccaaa ttatctgaca cagccctcac tataaaactg ttctcatata gctagatgtt
     2821 cattgattca agaactgcga ggacaaaagc aattcctatc ttatgatagt tgctctaata
     2881 cttcctctgt tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac gctgcatcca
     2941 ttgcttcttg gccaggagca ccaattgcat ctgtcgctac cacaacattg aatccactct
     3001 ctaccaattc acgcatatgg gaatcgctac acaagtttcc gttcattcca ccaagaatta
     3061 ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc attgctttct ggtccaaaga
     3121 ttttatgagg agagctgatg atcgtatttt catccaaata aggtttcaat tcttcgataa
     3181 aatcggctcc tgaaccttct tccactgatt catattgact ttttctagca aacatctgct
     3241 gctcaagcat catatgttct cctgcaccgc caaattgcca tttatggtca tgtgggtaaa
     3301 aataatgagg agaaataaag agtttgtatc ctttttcttt tgcttgtttt accatttcaa
     3361 tcatattttc gatcgtattg tatttttcta agatatcttt tgtcaaacca aagccaccgc
     3421 ctgctggttt caagaactcg acttgcgggt cagtgagaac tactgctgta tttttattat
     3481 tgaacatggt ggattctcca ttcgttattt attttaatac atcatttaga gcttcactca
     3541 ttgtcggatg agaatagatt tgatctctca ataacgtata aggaagtttt ccattcatag
     3601 ctagcgaaat caagttgatg acttcatgag aatcttccgc atagatactt gcccctagaa
     3661 tcaagtcagt ttcagggtcg actaaaactt tgaatacacc tttaggatct tctaatactt
     3721 gtgctttagg gacaccgctt gtcatatact tgaataattt gtaattgacg ccttgtgctt
     3781 tggcttgttt ttctgttaaa cctacattgg ataaagtcgg agtgataaat acactgtaag
     3841 gcacagcttt ccggttattc gttgttcgtt tattctcgcc cttcaactga tccataatga
     3901 ttcgataatc atctagagag atataagtga actgcaatcc gcctttgacg tctccaatcg
     3961 cccaaatgtt gtctgcatct gtacgaaggt attcatccac agcgattgct ccgcgatctg
     4021 ttagtttaac atctgtattt tctaatccaa gtgatgctgt acttggtttt cttccagttg
     4081 cagccaaaac ttttgacgct tggattgtta cttcttggcc atcttctgta aaggtgacgg
     4141 cagctgttga ttcttggtct gctacttgat ctacagatac acctagatgg aattcgattc
     4201 ccgcatttgt catgtcttct aaaatcattt gcgcgatatc ttcatcttca cgtggaagaa
     4261 attcagaatg ggcatcaaga acgacaactt tagatccgta ttcattgaac attgatgcaa
     4321 attccaagcc aatatatcca gccccgatga tcactaattt ttccggtaat tgttcctgat
     4381 ccatcgcttg cgtagaatca atcaagtagc gacttgtatt caatcctttg attggtaaaa
     4441 tgattggttc agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc actttttcac
     4501 cattattcgt cacttcgatt tgatggttag aaagaaaacg tgctgtaccg tccatcactg
     4561 tcccagttgc ctcatcgctg atcatatgat aatttttggc gcgaagcatg cccgtcaatt
     4621 tttctttccg agcaacagcc tctgtaaagt cgattcctct ttctccattg aaaatcaatg
     4681 atttagaagg aatacagccg atgttgatac atgttccgcc atacatacgt gttgactttt
     4741 caacgactaa aaccgattca ccttttgaag ctaacgcctt cgctaaagtc ttaccgccct
     4801 ttccaaaacc gatcacaata ttttgatatt tttccatcat ttttccctcg cttctttttt
     4861 acctaccgat tggtacaaaa atatgataac cgataattgt ctattctcca aatcatctgc
     4921 tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc acgtttttag tctggttcga
     4981 tttcctttat tcttctaaac aatcgttact tctacatcga tatttcctct agttgctttg
     5041 gagtaagggc agaattcatg cgcatcttct gcaatttttt gggcttcttc ttcgctgatg
     5101 ccatcgatat gcacttctaa tactacaccg atctgatagc cgccatcatc taaattataa
     5161 agagagacac gtgcttttac tgtggactct gctgtcattc ctgcttcttt gatcatatgt
     5221 tgtaccgccc cattgaaaca agaagcataa cctgctgcaa ataattgttc tggatttgtt
     5281 ccttctgcat ggattcctgg aggagtgatt ttcatatgca tcgagccatt tggtgcctcg
     5341 acttctccat tgcgcccgcc atggttgatc attgctgttt cgtatgcttt tttcattgta
     5401 ctgctccttt cgattgacca tttgctactt ggctataatt atagtgtatg acaagtaagg
     5461 cgctttctac aaaaattctg cttattcgaa taccagaatg ccgtcttcta ctatttttcc
     5521 aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata tttgttccaa aataaatagc
     5581 aatcttttct acataatctt ttatatgatt gattttaaga tacatatata ccagaaagtt
     5641 gaccccataa accaatactg ttaaaaaaat aaccatattc gttaccattt tttcctcctt
     5701 ttgctggtct ccctaccagc actcgattta accataccac tttttttgcc gtccaagcaa
     5761 ctagcctttc aaaaagactg gtttattctg ctgttaatga ttcaagatca gaaaaatgtc
     5821 cgaataattt tgctaatttt actaacgcca acttgttctt tccgaattca tcattttcta
     5881 tctgtaatac tagtaacggc tcatgtaagc ttaaacggat caaaaaccag ccgtttccgt
     5941 atggctcttc tagatcatat cgaatacctt cttcattttc cgggtcgaag ttcatccctt
     6001 tttcttcttc caacagtcgc ttcatttctt ctaatatctt gtttccttgt tcgcgtggtt
     6061 catcgctgaa caattttaat cggatttctt gtacttctac gggctgtttc aactctttga
     6121 tttgttcatc taatgtgata ccttttcttt ttaattctgg caaaagcatc aggatctttg
     6181 cgatgacata tgcaccatca tccagaaaat agttttcctt gaatgcagcg tgtccgcttg
     6241 tttcgatggc tagctgtgta tctttcccaa gacggttaag ttctaatgct ttattgatta
     6301 catttcgata gccggaaata taacgaactt ggatgcctcc tttttcttca atgaatcttt
     6361 gtagatgaga agaagtaggg gaattcgtca cgattcttgt tcctggatgc tcttttaata
     6421 cgattgtact taatactgcg atcaaattat ttcgattgat cactgttcca gaagcactga
     6481 caactgcaga acgatccaca tctgtatcga aaatgatccc aagatctgct tcatttgcta
     6541 aaacagcctg cttgatactt tccatcgctt ctttattatc aggatttggg atatgattag
     6601 gaaatcggcc atctggttcc aaaaattggg aacctgttga atccgcacct aagctattca
     6661 atactttttc agcaaagaat ccaccagcac catttccggc atcaacaatg attttccaac
     6721 catttagtgg tttgctttct agtgttccaa tccccttttt aatttttgtg actaaatctt
     6781 gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg ctttttattt
     6841 tttgctgctg tgataggata aaagcaatat cttctttttc tgctcctcct tctgaggtaa
     6901 agaattttat tccattataa taatagggaa gatggctagc agtcagcata accgcagcat
     6961 cgcacgagaa ctgttcaaat tgagtggaca taaacatagc aggcgtagta gctaattcga
     7021 aatcgatgac atgtatccct tgttctttca gactttggat caacgcctct ttcagtgctg
     7081 gtccgcttat cctactgtca tgaccaatcc caatcgtcca ttttttattt ggatcggttg
     7141 atttcttctt tttctttatc cagtttgcta gtccccagcc gatctttttt accatgtctg
     7201 tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat tcctcgaata tccgatccat
     7261 tttgcagcgc tttcatatct ttcatataaa agtcctcctc atttattccc tcattttttc
     7321 cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg caaataataa tgaatgtatc
     7381 tacaccggcc atcgttccga caacttcagg gtaagccact tcatctagcc aattcgttac
     7441 cacatcggca ccatttcgtt cagtcaatac gataaccatg aactgaacag actggatacg
     7501 caaaacctcg cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa cagatggctg
     7561 gttaaataat gtatagcgta ctattttgtt ttcattttga ctttttacta acttcatttc
     7621 tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat gcagccgcgc
     7681 aagcagctct tcctgtgtcc caataggttg ttctgtaatg atctggcgga tcagtgcttg
     7741 tcgctgtttc tttttcatat tagacacctc tcaatgttga ttatagcaaa agataagtaa
     7801 tatttcttca tattttggta ttttttactt ttattttttg ttttcattca ggttgctaac
     7861 ctttttctat tttaacacgc aaaatcgtct tcattttttc aggtgctgat tttcttggat
     7921 cgctgatgta aatctctttg tgttctttgg aagctctttc atagccttct tttgcgatga
     7981 atgcttctaa tttctcaaac gttgctggtt cttcggcata gggcccgacg tgtaagattt
     8041 gggcacaaag accttcttcc atctttttga atactacttg ttctttcaag tcttccggaa
     8101 gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag tcaggctgtc
     8161 ggatcatcaa ttgataagaa aaatgttctt ttttcatcac tggttcatgc aaatactttt
     8221 cttgcagtcc ccattgtcct tccagtggat agacagtata catctgataa tcaggaatca
     8281 gccaattttt tttaggcgac atgcgtattg catagctgac tgcatataga caggctactc
     8341 ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct gatgctaaag aatgattttt
     8401 taggaacctc tagtatttgc gggctttttt tcatactata gctcttgtct ttacgccatt
     8461 caattttttc catttttctt cctccgcgaa attccattta gttatttatt atcatacctc
     8521 tttttgagtg acttggctaa tcgtgttgac actatttttc agtttgtgct agaataagtg
     8581 aagtaaataa atcgtacaga taacacgttt cggcgtgtcg agagagaggc ggtttctatt
     8641 tttataatag aaatcactct ctttttttac gaaatttttt agaggtgatg taaatgacaa
     8701 aacaaaaaca gctgctttta gtacctgcag taattgcaac agggatgatg tcttttgcgg
     8761 gcgtattgat cgagacggcc atgaatgtaa ctttcccaac tcttatcaga cagtttgatt
     8821 taacgactgc acaagtccaa tgggtaacaa cgatttatct tttgatgatt tcgatcattg
     8881 ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa aaaactcttt ttagcttcaa
     8941 atctgctttt cttggcaggt ataatgatcg atttcttctc tcctaccttc atggtcctat
     9001 tgtttggtcg attgctccaa ggtgcagcga caggtatcgc tcttccgttg atgttccata
     9061 ttatcttgac atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc ggtactttga
     9121 caacggccat tgctccagca attggcccca cttatggagg cattatgact tcacatttta
     9181 catggaatca tatcttcctg tttcttgtgc ccgttttgat tctttctttg attatcggct
     9241 ggtttgctat tcccgagatg gcagtacaaa aaagtggtta tttggattgg ataagcgttt
     9301 taggaatttg tcttttgttt agtggaagtc taacattttt gagtacgttg ggaacagttt
     9361 ccagctggat tgctttaatc atcggcggta ttggctggtt ccttttttac tatcgaagta
     9421 aaaaagtgga gcatccactg atccgactgt ctattctttc aaataaaaca ttccgcttgt
     9481 ttttgattgg cttcttagtt tatcagtttt tattacttgg tgtatctttt gtcttgccga
     9541 atttccttca gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg atgcttcccg
     9601 gcgcagcggt tggtgctttg ctttctcctt tttccgggaa aatgctggat caatatggtc
     9661 cgaagaagcc aatttttgct gggctgattc tttctttgat tggttggcta gcattattcc
     9721 tactcatagg acacgcttca gtgggtgtat tgattgcttg tcatgtgttt tatatgatcg
     9781 gtatcggatt atcttacagc aatatgatga ccaccggaat gaatgcactg aatgaagaac
     9841 tacaaggaga cgggaacgca atgttcaata ccttgcagca gttttctggc gctgtggcta
     9901 cttcattagt agcggtcatt atcaattacg tccagcatca tacttcccat aactatgaag
     9961 tgtctactac attaggatca aaagcagcat taggcgtctt gttgctgttg cttctcgtaa
    10021 gctttgctcg attcgcctat tatctgttct ttgctaaaaa agcgtagatt ccatatacgg
    10081 caggtgttgc atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag gaggcttttt
    10141 tatggaaaaa attgccatta tcggtggcgg gatcattggt atgaccctag ccagttactt
    10201 agatacatca aaattcaatg ttgctttatt tgatgaaggg acaggacagg caactaaagc
    10261 tagcgctggg attatctccc cttggttgtc gaaacgcaga aacaaacaat ggtatcgtct
    10321 ggctaaagac ggcgctgctt ttttccctaa acttataaaa gatttccaac ttgatcaaac
    10381 gatttacgca caatcaggga cacttttgct tcgacctgaa aaagatttag ctgaattggc
    10441 tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg aatggctttc
    10501 acctgaacag acatctcaag cacttccgtt attgaaacca gcagtttcac tgcgtatcag
    10561 tggtggtggt cgtctggatg gaaaagcata tttagaacat cttggtcagc gtttagcaca
    10621 aaaacaaatg gagcagatcc aggcaaaagt cactttgcat aagaagagcg attcttggct
    10681 catcaaggga ccgtttggag aatacacagc tgatcgtgta attttgacac ctggcccggc
    10741 acttaagcct ttattacaag aattaggcta tgctgcagat atacgtccgc aaaaagggca
    10801 gctcttagca tttcagacat cttataagat gagtcaagac tggcctgttg ctatgcttga
    10861 cggagaagct gatttcattc ctttcatgga tggaaagatc cttttaggtg ctacccatga
    10921 aaacgatcaa ggctgggatt tggctcctac tagcgaagct tataagggat tgactaagag
    10981 tacagaacag ttcttgactg atccagatca gataaaaaac tggccatacg agttccgtgt
    11041 aggtacccgt gcttatacgt cagatttttc cccatttttc ggcgtactgc cagatgatcc
    11101 aagcttactg gctgctagcg gtctaggctc ttctggcttg accacaggtc cttatatcgg
    11161 ctatctatta gccaacttct taaatactgg tcattggcct acggaagatt atcaaaagga
    11221 catctccacc tatattacga aaaatccatc atagaaaaac agctttttca agtaaggatc
    11281 ccgagcgata ttgcgcatgg attacttctt gaaaaagctg ttattttagt tcaatttctg
    11341 aatcaaattc tccaaacttt cttttgcttc atctggcaag agatcttttc ctatctgagc
    11401 ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat aggacgtatc
    11461 ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg atgtcttcaa atggaccaaa
    11521 cgacttgaat tctgccgttt gttgcacgat tctttcaacg attgacaatg tcgtttcagg
    11581 tttgattttt ttatcatcga aatatccagt attcaggaca tagcaggctg tttgctgttt
    11641 caaaaacaac tctcgaaaag cttgatagtc ttccctaaga ggatagacac ggaaaggatt
    11701 agcaaatggt tcaatgacta gctcatctag ctgtcttttt gtagtgatat tttccgcgtc
    11761 tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct aaaactgggt cattgatctt
    11821 gataattggc ggaagcgaat catctttcat gatccaaaaa acagcatcga tcttttcgct
    11881 aagatgatcg actcggtcgg gtgtcgcata acgtgatttg actgtccgcc catttccgtt
    11941 tcgtatatct cctgttacta ggactttttc tcctttactt gtacatgtca cgcccacatt
    12001 ctgacaagta aggaaagaag aaatggccgg atcatcagac ggatagtctt gtgttttatc
    12061 aaagtaagaa ggttccaagg cagtagcaaa accatttttt cgagaaataa cgaaagcatc
    12121 gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa tcgttgattt
    12181 tccagaaccg gacaacccaa atacagccat cgtataggtc tggtcattac tagcatattg
    12241 tttcatccca ccgtggcagg caataaagcc attgcgattt cccaatgccc aagcaagcgt
    12301 caaagtcgct ttttttaatt ctccaaagta acgtaatcct agcacagccg ctgtattatg
    12361 gagagcatca aacaaaatga gtccatttgg aaatgtttca tgtgtccatt cgggatcaca
    12421 aaagatataa agatctccct catcaaagga aacagacttt tgataagtct ctaagatctt
    12481 cgaattcgct ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa atccttctgg
    12541 aatcagcaaa tgactttgca cagaaaattc ttcagatagg ccaacgatta cttttgtatg
    12601 gtaaaactgg cgcttgcgac tttgatacac agcttctctt aaaattttct cgtatttgct
    12661 ttgttccttg tctaagtcct caaagatccg ccgagcactt gctgttcttc ccacaatgct
    12721 tccatcattg aatactagtg tcctaacatc agctggtaaa tctagttctt cagcatgtga
    12781 gattgttaaa tctgtgatga tcgttcctgg actccctttt gctagttgat aagctgtctt
    12841 cctatcaaaa acttcatgaa cattattacc gtaaaatgtc gtttctacaa tcgatcgaat
    12901 actggataac agtggatttc cacttgtgat ttcgtttaat tcgagactct cttttgtact
    12961 catggctcct cctcattccc taaacaaaaa aacgttttct ttattataca ttattcgagt
    13021 ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg
    13081 agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc ttagtaccag
    13141 ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga tgttacatat
    13201 tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta tgaagcatct
    13261 aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc tgattctttt
    13321 tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc tggggcagca
    13381 ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt tgctgtttca
    13441 gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga atcatttgaa
    13501 gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat cgaatttttt
    13561 tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat cttagataat
    13621 gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt tcctacaaac
    13681 attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa tgaattcata
    13741 ttaaactcct ttttttctcc gttttttatt ctttcgacgc tattaa
//
LOCUS       DAGCYL010000068        13647 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.069, whole genome shotgun sequence.
ACCESSION   DAGCYL010000068 DAGCYL010000000
VERSION     DAGCYL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.069"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            74..316
                     /gene="yaaA"
                     /locus_tag="MB216_001478"
     CDS             74..316
                     /gene="yaaA"
                     /locus_tag="MB216_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBT4526319.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            303..1427
                     /gene="recF"
                     /locus_tag="MB216_001479"
     CDS             303..1427
                     /gene="recF"
                     /locus_tag="MB216_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBT4526320.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1424..3370
                     /gene="gyrB"
                     /locus_tag="MB216_001480"
     CDS             1424..3370
                     /gene="gyrB"
                     /locus_tag="MB216_001480"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBT4526321.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3390..5861
                     /gene="gyrA"
                     /locus_tag="MB216_001481"
     CDS             3390..5861
                     /gene="gyrA"
                     /locus_tag="MB216_001481"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBT4526322.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6097..6396
                     /locus_tag="MB216_001482"
     CDS             6097..6396
                     /locus_tag="MB216_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBT4526323.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6446..6958
                     /gene="ssb"
                     /locus_tag="MB216_001483"
     CDS             6446..6958
                     /gene="ssb"
                     /locus_tag="MB216_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBT4526324.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6984..7220
                     /locus_tag="MB216_001484"
     CDS             6984..7220
                     /locus_tag="MB216_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBT4526325.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7363..9336
                     /locus_tag="MB216_001485"
     CDS             7363..9336
                     /locus_tag="MB216_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBT4526326.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9342..9794
                     /gene="rplI"
                     /locus_tag="MB216_001486"
     CDS             9342..9794
                     /gene="rplI"
                     /locus_tag="MB216_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBT4526327.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10068..11435
                     /gene="dnaB"
                     /locus_tag="MB216_001487"
     CDS             10068..11435
                     /gene="dnaB"
                     /locus_tag="MB216_001487"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBT4526328.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12215..13507
                     /locus_tag="MB216_001488"
     CDS             12215..13507
                     /locus_tag="MB216_001488"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBT4526329.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4549 a         2467 c         3002 g         3629 t
ORIGIN      
        1 gataaatttg ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa
       61 tgagggatgg gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca
      121 aatgttgaaa gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga
      181 aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg
      241 catgatggtt gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga
      301 taatgaggct gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg
      361 aattttccaa gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt
      421 tggaaagtat ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat
      481 tgattggctg gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca
      541 ctcttccatt agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg
      601 aacagaaaaa gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag
      661 atctttcttt agtaaaaggc agcccgcaga ccaggcggaa gtttttagat atggaattag
      721 gccagatcaa tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa
      781 gaaaccagta cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata
      841 ttttgtccga acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg
      901 tcaagcgatt ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc
      961 agctggaaat cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa
     1021 tccaagaaca atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa
     1081 caacgacagt tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc
     1141 agacttatgg atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa
     1201 tcgatttgat taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga
     1261 gcgagctgga tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa
     1321 cgttcttaac aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga
     1381 ttttttatgt tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag
     1441 tttagtagaa cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga
     1501 agccgttcgt aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca
     1561 ccttgtttgg gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa
     1621 aatccatgtg attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat
     1681 acctgtagat atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt
     1741 acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt
     1801 aggatcttct gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg
     1861 aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga
     1921 acaaacggat cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga
     1981 gacaactgag tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa
     2041 ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata
     2101 ccattatgac ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat
     2161 ttttccagaa cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat
     2221 gcaatataca gacggctacc attccaatat catgagtttt gccaacaata tccatacgta
     2281 tgaaggcgga acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta
     2341 tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg
     2401 agaaggattg acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac
     2461 aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca
     2521 tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt
     2581 actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg
     2641 tgctttagag attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga
     2701 aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg
     2761 taatcgagag tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc
     2821 aagcatggac aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac
     2881 agggtttgga gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac
     2941 tgatgccgat gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta
     3001 catgcgtcct attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt
     3061 aaaacaaggg aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa
     3121 acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact
     3181 tggagaaatg gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat
     3241 ggcccgcgtc agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg
     3301 ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt
     3361 agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg
     3421 atgtcaatct aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg
     3481 tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat
     3541 acggcatgaa cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg
     3601 tcggagatgt catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg
     3661 tacggatggc acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg
     3721 gttctgttga cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa
     3781 tcgctacaga aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg
     3841 atgatacaga aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg
     3901 gaacaacggg gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg
     3961 tagtagcggc tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg
     4021 aagtattacc tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc
     4081 gaagagcgta tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg
     4141 agatgccaaa tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag
     4201 caaaattgat cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag
     4261 atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg
     4321 ccagcgcatc tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg
     4381 gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc
     4441 ttgaaaacta tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag
     4501 aaaaagcaga agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg
     4561 atgaaatcat tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga
     4621 tcgaacggtt cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc
     4681 gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga
     4741 ttgaagattt aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg
     4801 aattagcaga cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg
     4861 aggtcttaag tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga
     4921 caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg
     4981 gacgtggtgt ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt
     5041 gttctaccca tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag
     5101 gatacgaaat tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag
     5161 gtatcgattc gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag
     5221 gccactatct cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt
     5281 ttgctaacat ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg
     5341 tcaatgtgct actgacagat ggtcaatcta atatcattat tggaacacat aacggctact
     5401 ctgtcacttt caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag
     5461 gaatacgcct gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag
     5521 aagtattagt gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag
     5581 tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac
     5641 tagctggatt gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg
     5701 tcattatccg tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac
     5761 gtttgatgaa gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag
     5821 ctgcagaaca aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt
     5881 aagctagcaa gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt
     5941 tgaatttctc ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag
     6001 tgtataatca gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa
     6061 cctaagagcc gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc
     6121 atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc
     6181 gatgcaatct tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc
     6241 cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct
     6301 tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat
     6361 atcattcgtc acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag
     6421 aaaacgatga gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac
     6481 gaaagatcca gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc
     6541 tgtgaatcgt aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt
     6601 gatttggcgc aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg
     6661 tgttactgga cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt
     6721 gacagaagta gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg
     6781 ccaacaaagc ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa
     6841 taactctaat caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc
     6901 tgacccattc ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac
     6961 gaatttgata ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta
     7021 aagtagacta cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac
     7081 taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg
     7141 ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac
     7201 ctttcgttag cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct
     7261 attcaattcc tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa
     7321 agttatggta aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat
     7381 catttatccc cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc
     7441 ttaattttac catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt
     7501 gttcgctatt ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag
     7561 caggcttcga ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga
     7621 gtgattgctt atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa
     7681 atcaaagatc aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa
     7741 gaaaaaaaga aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca
     7801 gtaaagggtg ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag
     7861 acgaacagtc agccggtgat tggtatttta tctattgata actatgcaga tgccattgat
     7921 aaattggatg acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac
     7981 tggatgaatg aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta
     8041 gcacagatga tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt
     8101 cgagcagaag ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc
     8161 tcaagaaccc tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc
     8221 cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat
     8281 ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc
     8341 gctttgcgtc ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg
     8401 gatatggatg ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa
     8461 aaagcttaca tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa
     8521 gaaatccata agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag
     8581 ctgaaaaaag aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc
     8641 tctcagggcc tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg
     8701 gaagagtttc caagtaaacc tctgctgact tatatcgaat cttcagcttc ttcagcttca
     8761 gagctagttt ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt
     8821 gaggccacat tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca
     8881 actgcacgaa cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta
     8941 gtacaatatt tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct
     9001 ggaagcgact atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat
     9061 aatagtgtta tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca
     9121 gctttcgtca ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc
     9181 acgatcaacg tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca
     9241 gctactcaac tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc
     9301 aaagaaaata tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac
     9361 aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac
     9421 aaaattatct aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt
     9481 tagcaggaca aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa
     9541 agttaaaaga attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg
     9601 aagacagccg tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac
     9661 aatacaaaat caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag
     9721 gctacacaaa cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg
     9781 tcgtacaaga ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa
     9841 aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa
     9901 atcgatttca gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac
     9961 gagtataata aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct
    10021 ctcattttct tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt
    10081 ggcaggatcg aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt
    10141 ttcttgacgc agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc
    10201 gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga
    10261 ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg
    10321 gtttaagtta tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact
    10381 atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa
    10441 tcgttacgaa aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac
    10501 gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag
    10561 tactgaactc cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag
    10621 gtctaccaac tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac
    10681 taatcattct tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc
    10741 aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag
    10801 aatctcttgt aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta
    10861 caggtcagct ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc
    10921 gagcaaatat ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat
    10981 gtcggaaact agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt
    11041 tgattgaagg taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac
    11101 tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg
    11161 gagtagaaca gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa
    11221 ttgaacaaga tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag
    11281 gcggagatga cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg
    11341 agaagaaccg aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata
    11401 agttttcgtc gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa
    11461 tcccgagagc tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa
    11521 tttaaacaga tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat
    11581 aaacggtgtt caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa
    11641 ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt
    11701 ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga
    11761 gaagcagttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc
    11821 tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat
    11881 atgagaagct atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa
    11941 cctctgattc acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa
    12001 aatttgagaa gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta
    12061 caacctcttt tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata
    12121 aaataaaaat aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg
    12181 ttcaggatta aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac
    12241 gcaatggggc gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt
    12301 gattgcacgg taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac
    12361 ttataaactg catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg
    12421 aaacggcgtt gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa
    12481 aggtgtagaa acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca
    12541 tatcaaattg gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat
    12601 taaaggaatc ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga
    12661 cttattagat aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg
    12721 tcaatttgta aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata
    12781 ttatgaatac ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga
    12841 tgctttggat gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat
    12901 cgaccaggga acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat
    12961 cggaagcggt gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac
    13021 gtcacgtgtc ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat
    13081 ccgcgaagta ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt
    13141 tgattctgta gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa
    13201 ctcgatcgat gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga
    13261 tggcgaacta atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat
    13321 ctatgaagaa ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact
    13381 gccagaaaac gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc
    13441 aacattttct gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca
    13501 aatctaagga taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt
    13561 ttatctatac gataataata acttgaacac tgtttattca gaataaggga ctgtgacaaa
    13621 aaccgtgtca cagtcccttt ttccgaa
//
LOCUS       DAGCYL010000069        13592 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.070, whole genome shotgun sequence.
ACCESSION   DAGCYL010000069 DAGCYL010000000
VERSION     DAGCYL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.070"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..591)
                     /locus_tag="MB216_001489"
     CDS             complement(<1..591)
                     /locus_tag="MB216_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBT4526330.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKS"
     gene            complement(839..1675)
                     /locus_tag="MB216_001490"
     CDS             complement(839..1675)
                     /locus_tag="MB216_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBT4526331.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1686..2615)
                     /locus_tag="MB216_001491"
     CDS             complement(1686..2615)
                     /locus_tag="MB216_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBT4526332.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2807..3160
                     /locus_tag="MB216_001492"
     CDS             2807..3160
                     /locus_tag="MB216_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBT4526333.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3234..3890)
                     /locus_tag="MB216_001493"
     CDS             complement(3234..3890)
                     /locus_tag="MB216_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBT4526334.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4116..6050
                     /locus_tag="MB216_001494"
     CDS             4116..6050
                     /locus_tag="MB216_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBT4526335.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6136..7359)
                     /locus_tag="MB216_001495"
     CDS             complement(6136..7359)
                     /locus_tag="MB216_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBT4526336.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7353..9251)
                     /locus_tag="MB216_001496"
     CDS             complement(7353..9251)
                     /locus_tag="MB216_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBT4526337.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9245..10363)
                     /locus_tag="MB216_001497"
     CDS             complement(9245..10363)
                     /locus_tag="MB216_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBT4526338.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10446..11279)
                     /locus_tag="MB216_001498"
     CDS             complement(10446..11279)
                     /locus_tag="MB216_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4526339.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11272..12189)
                     /locus_tag="MB216_001499"
     CDS             complement(11272..12189)
                     /locus_tag="MB216_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4526340.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12416..13540)
                     /locus_tag="MB216_001500"
     CDS             complement(12416..13540)
                     /locus_tag="MB216_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBT4526341.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         4237 a         2718 c         2234 g         4403 t
ORIGIN      
        1 tgatttttta tattttttaa ttgtgccgtt tacgagaatc tcttcatctt tcaatactag
       61 gcctatttta tttttataac ctggcatgct cattagataa aggatttcat cgtaatggat
      121 atagtgatcg ctattaccat aacggacagt tgtccacctt tttttcgtag catgttgaac
      181 aggatagttt tctttttctt tcaataagta actttggatg ctttttttat ttttttcata
      241 agtgaattct tttatcaaat aaccactagg cgtaatcttt cgattaataa cagttattgc
      301 tttattcgga tgagacgtta gataaacaat tttacacttt tcattctttt ctctaagcat
      361 ttcgcctagt tcgataccgc tataataggc gttcaaatcg atgtctagga aaaaaatatc
      421 tgaggaatag atttcattat ttttaatgaa ttcaacaaat tgtataacgt tcaatccctg
      481 aacgatctca aatttccaag gacttaataa aattctggat tcttccaaaa tttctactag
      541 cattttttca aaaaccaaac tatcatccaa aataaatata cgcgttacca taaaaagtac
      601 ctccactcga tattttacta tcaaaatcat ttcatacaac ttttcaacat acatgttagg
      661 aataaattgt aattctcttc acaatctttt ccaacacctt tgaaaccgat atcaaatact
      721 ttcaggatgc tgcttctaaa ataaatatca aacaaatgaa tacatcatgc aaaaaaagcc
      781 agacaaccga cgtccttgcg tgtcagctgt ctgagctatg tttatttatt ttgttctttt
      841 aaaaagaacg atacacatac tgatttttcg gagcagagac tttctcagcg tttgtaacaa
      901 ctacagatgg gatttcacgc ttgaatggtt gataatagtc tgattggatg gttccttcta
      961 ttttatacca ttcatcattt ttaaccgtta ctccttccgg catatgcgtc aacaaaccaa
     1021 agactcctga atctgccaca caatggataa tgccgaatcg gaacaagaag atatctgtat
     1081 tgctatcgtt cggtgtttgg taaacaaatc cttcatagct gatttttttg ccaatgaatt
     1141 cgctagggta attataaatt agctccataa cttctaaata gttttcatct gtgatcttga
     1201 tagtttgctt atccccatac ttttctttca actcattcat ttggtctaag tagtctgatt
     1261 tgttgaaata aatactggta tccggcttta agtattgtgt ctgcatctcc ggatccccta
     1321 ctgattcttt acttactggg aaattaaatc ctttggcttc tacgatcgta gtgtcgagac
     1381 taactgttgg aaacaacgtc cccacgatta ctggcaaagc tagcagtaca taagcgattc
     1441 cacgttgata aggttttgac aacccatggt catgatcgtg ctcgtgtgcc ttttcttctg
     1501 cttttccgtt tttgacccac aaatacagct gaactaaagc caaaagagca gataaaatca
     1561 ttgaaagaat agctaagtag cgataatgga cattgatata ttgatcgagt ttgccagaaa
     1621 cttgcaagta catcatcaat tcgaaatacc cagataaaat caaaaaccgc agcatatttt
     1681 ccctcctaaa taaataatgt gaatccaata acgctaaggg caatcagtcc gatcagcgat
     1741 aaaataaagc gtcctgtaaa atatcgtttc atcatcagta gatttttgat atcgaccatt
     1801 gggccaaaga ccaaaaaggc agctacaggt gctgtgccga acagacttaa caaagaagat
     1861 cctataaacg catctgcttc tgagcaaaga gacatcgtaa aggccaataa cagcatgatg
     1921 ataatggcta aaatctttgt tgagccgagc gttagcataa ttctagttgg taaataagtc
     1981 tgcatcgcag ctgcgattaa tccgccaatg atcaaataac gacctgtatc aaaaaattcg
     2041 tcgattgaat gtgtcaacac atgcccagtt tgatggatca gctgattttt tggttcattc
     2101 ttctcacttt gttcttcttc atggacatga tgatggtgat tttctgctac tttagacgtg
     2161 attttttctt tcaatacagc gtctttttga aaataggcaa gccagacacc aatcacaagg
     2221 gcaacaaaaa agctgcctat cacccgccag cttgccatct gccaagaatt gccaaatgcg
     2281 ataaaagtgg aaaaaatcac gattggattg atgatcggag cagtcagcat aaaagcaaac
     2341 gctgtgtgaa ctggtacgcc ttttttaaca aattgatgaa cgatcggtac gatgccgcat
     2401 tcacatgaag gaaagaaaaa tcctaagatc gaaccagtaa aaatagagaa gatacgattt
     2461 tcaggaagcc atctcttcac acgttctggg gtcaaaaata cttgtaaggc gcctgagata
     2521 agacagccta ataatacgaa aggtaatgct tcaataacga tcgataagaa aatcgtactc
     2581 atctgcaaaa ccgaatttgg aataaaagaa aacatgagtt tcctcctttt tatttctagt
     2641 tcattcgctt tgcaaatata cgcttttatt caaaaaaaac aagttgctct gtaaattttt
     2701 acacaactct tattttttac caaagcactt caattgactt tttctgttat tcccattaaa
     2761 ttaaggtctc atacatatca cttctatcat aaaagggaga ataaaaatga aacaaaccac
     2821 taacttgact gaagttcaag acatactgca acagtcatcc gttgccgtca tttatctttc
     2881 gatgcctaat tgcagtgttt gtcacgctgt tcgtccacga ctagaggaac ttctcacaca
     2941 ttatgatatt ccggcacttc atttggatgc atttgaaaca ccagaagtag ctagtaaatt
     3001 tgaagtgctg actgcgccag ttgtcctgat ttttcatcaa ggaaaagaag tctttcggca
     3061 agcacggttc atcgacttta aaaaaatccg ttatctacta gatgaactga cagcagaaga
     3121 tgattccctt tcttatgaag aaatcttccg aacaaaataa tcgttgtcca aacagaaaaa
     3181 agcgattgaa caagtgaaag ttttgccttc atttgttcaa tcgcttttta tttttattct
     3241 gctgtatttt cggaatgcgc atttgcgtca gaatgcaaga aatagatcaa ggtttgcaat
     3301 tcattcgtca aatcaacatt ttggatcaat acatctgacg gagcattcaa tcgagcagcg
     3361 gtaaagttca agattccttt gattcctgct tccgttaact gattgactac ttcttgtgct
     3421 ctttcagcgg gtatcgttaa aattgctact tcgatttgtt ggatcttgat ttgttctgac
     3481 atatcttcca ttggataaac ggggataccg tctacgatgc gcccaacgat ttcctcattc
     3541 acatcaaaag cacaactgat acgtatgcta ttactttgat ggaatttgta ttttagtaaa
     3601 gcacttccca agttccctac accgatcagc gcaacatttg tcaattgatc gtcgtttaat
     3661 gtctttgcaa aaaagtgcat aagatcttct acatcataac catacccacg ttttcctaat
     3721 tcgccaaaat aagaaaaatc tcgacggatc gttgcactat ctacttgaac ggcttcactt
     3781 aattcagttg aagacacttt tttctttcca gcattattta aaatccttaa atagcgataa
     3841 tacaaaggca gtcttttagc tgtcgccttt ggaataatat gatctttcac gaaatgacct
     3901 ccacgtattg tctaattaca tctttgattt ctccatcata gcagtattta taggaaaaaa
     3961 tcaattccat tgctcagata ttgtgaaatt ttattcttta ttcatttatt tatcatttct
     4021 gcaaatctcg cagtataatg gaatttatgg tagactacta attgaaaaat agttttttat
     4081 ttgaggagaa ttcacaaagt agaaaagagg attccatgat attattacaa gccaatcaga
     4141 ttgctcgcca ttttggtgca gaagtcttat ttgaaaacat acatttagaa atcagtacag
     4201 gcgctcgtgt cgctttagtt ggcagaaatg gcgctggaaa atcgacttta ttaaagatca
     4261 ttgctggaat tgaagcacct gatgaaggaa agatcgctaa aaataaaaca gcgactctcg
     4321 gatacctcgc tcaggacaca ggacttgatt ccaatgaaac agtttggaac gagatgctga
     4381 aagcttttga acaagttaga caaatggaag agcggatgcg cgagctagag attttcatta
     4441 gcgaaggtac gccagacaca cctgcttata gttcttattt aaaagaatac gaccggctgc
     4501 aacacgattt ttcagatcga aacggctatg gttatgaaaa tgaaattcgt tccgtattgc
     4561 atgggtttaa attcgattct tcattttatg atcagaaaat cgatacttta tcaggcggac
     4621 agcgcacacg tttagcttta gctcgcatgc ttttgcaaca ccctgacatt ttgattctag
     4681 acgaaccaac gaaccattta gatatcgaga cactttcctg gcttgagaat tatctgcaaa
     4741 gctatactgg tgcattattg attgtttccc atgaccgtta ttttttagat aaagtagtca
     4801 atgaagtcta tgaattaagc agaagaaaaa tgcgccatta caaaggtaac tattcgaaat
     4861 atctagattt gaaagcagaa cagttagcaa gtgaatggaa ggcttatgaa aagcagcaaa
     4921 cagaaatcca taaattagaa gacttcgttg ctcggaacct tgttcgcgcc tctaccacaa
     4981 aacgagcgca aagccgcaga aagacattag aaaaaatgga ccggctggat cgtcctcaag
     5041 gggatgaaaa acaagcaaac tttctattca gtatagaaaa gccttctgga aatgtagtct
     5101 tgcaaatcga agatgccgct atcggatatg ataatgtcac cttatctgaa ccaatcaatc
     5161 tagatatccg tcgtcaagaa gcgattgctc tagtaggtcc gaatggtatc gggaaatcga
     5221 cgttgctgaa atcaatcatt caccagatcc cttttatccg tggaaaagaa acgctaggga
     5281 caaatgtcac catcggctat tacgatcaag gtcaagctga tctgcataca aacaagacga
     5341 tcctagcaga attgtgggat gagcatccta ccactccaga aaaagagatc cgaagtgctt
     5401 taggtgcttt cttattttct ggcgaagatg tagaaaaacc aatccctctg ctaagtggcg
     5461 gtgaaaaagc acgggttgct ttagccaagt tagcgatgaa caaagaaaat tttttgattt
     5521 tggatgaacc aacaaaccat ttagatattg acaacaaaga agtattagaa aatgcattga
     5581 tcgattatga cggcacactg ctttttgttt cccatgaccg atatttcatc aatcggatcg
     5641 cgacaaaagt cgtcgaactc tctgaaaatg gcagcaaagt ctatctagga gactatgatt
     5701 attatttaga gaaaaagcag gaagaagaag aaatcgccga attgttggca caagatgagc
     5761 cagaatctgc tcctgctccc acgaacaaat ctgattatta ccaaagcaaa gaacagcaaa
     5821 agcttatccg tagccttcaa cgaaaaatta ctcaggttga agaagaaatg gcgcggatcg
     5881 atgaatggat cgatcagttg gaacaagaaa tggtcgcgcc agaaaacctg gaagatcacg
     5941 taaaactcaa tgagctgaac caagatttag aagctgctag acaagatcaa gaaacaaaat
     6001 tgacggaatg ggaagaacta agcttggaat tagaagaata tgaaaattaa cgaaaaaacc
     6061 aaaacagaaa ccattcatgc tgttttggtt tttctttttt tattttatca aacaaaaatt
     6121 ccatatttac ctttttcacc aataagaaga ccaattacta gtcattctca ctaaagcttc
     6181 taaataatag taatcacccc aaatacaagg tgtatttacc ccattatttt tgggcttatt
     6241 ataaacagac tgtgttaaaa agccatcttc ttcttccgtt gtagcatatc tttctttcaa
     6301 ttgtcgcata attttccaaa cagcagactg gtataagttc ttttctgttt tgtctaatag
     6361 acctgattct attaattcta ataatccaca aaccatgatc accatactag atgtatcttt
     6421 ttcttctctg ctagaatcat caaagatcaa atcccaataa caaatggtat cttctggcaa
     6481 ccgattcata aaataattgg ctaatatttt gcttaaatca aaatattttt gttctttcaa
     6541 ataacgataa cttaatgcaa aaccgtaaat cccccatgct tggcctctag cccaacagga
     6601 attatcacta taaccttgat gcgtacttcc atatcttggt tttccatctt tgacatccat
     6661 ataaaatgta tgataagttg aaccatcttc tcttactaaa taatctgctg cattttgaat
     6721 atgagcctga gcagcttttt tatatttttg atttccagtt tcttcactcg cccaatacaa
     6781 taatggaaga ttcatgcaac aatccatgat cattctacct tgttgtgaag gatcactagg
     6841 atctccccaa gcttgtataa ctcctgcctt ctcccaaaaa cgctgaagta atgtttctgc
     6901 agcatcaatt gaagcttcct tcatttttcg atttcccgtt attttataat ctgctacggt
     6961 tgataaagaa tacaaaaaac caagatcgtg atgattagtg ataatttgac agtcaatcct
     7021 gtggataaaa tctatatctg ctttttcagc tgtttgttga aataaaggat cacccgtctt
     7081 ctcaaataac aaccaattaa ttcctgtcca aaatccttct gtccaaccaa tattgtctct
     7141 taatggataa cgtcctttta taggacactc gcttgggaat tgatactgac tgttactgat
     7201 acgtttgatt aacctctccg tgtgtctgcg acaacgctcg atctcattat tccattcttt
     7261 ttccattaat tctttataac attcatttat ccatttttct ggatgtatta tctcttcaaa
     7321 catcacttta tcaagcattt ccccaccagc ctttagcata attttagtct ttttttattg
     7381 tttttatttt ggatagttag cccggttgtc ccatcaaaat caattttggg acgattgttc
     7441 catacttcta aactataatc ccgatcttga tgagcaacca ctcctgttcc gagccaatgg
     7501 gttccttttc caaatgtacc ctctaatcca ggaataaaag tgttttccca tgtcatcaag
     7561 tttgtattgg gcaaacccat aatagctgtg gattttcgtt taatagtatc gtcacttaaa
     7621 cagataatac ctgaaaagcc agcttcactc accctatagt tttccttgtc ttcttctatg
     7681 attattcctt ctttctcagg agaacattca atagcaaacc ctgtctcata tagaacatat
     7741 tctgtatcta attctacttt gtgtacgcga atctgccagc cttcaaaagg aataagccaa
     7801 gttctgattt ttgcctgtaa agtaggttcc catgtagagg caagtacctc tttcttacat
     7861 acaatgtctc gtacattatt tcgaacgatg aactgtccat aggttttgat gctctcattc
     7921 ttaaaactgg tcgtgatata tgtgtccgct cgctgaatcc ctagcattga atccattgcc
     7981 atttctgctt tcaattgatt cgctcgcggt atcgaaaaac caaattgact agaataagca
     8041 aattttaaat atttttcatt ggtcaatgta tggaaatttg gccctaattg tcccgcattc
     8101 aaaaaaaatg tatgtccatt atcatgttgt atgagcaaat tggcttctga aagaagttta
     8161 gttccttcac gaataggcat aggcatttct ttagccgtcc aatactcatg gtcttcaggt
     8221 agcgccaaca ataaaaaaat cttattcagc cataatggag ataacgtact attatagggt
     8281 tcagtcaatg tcaattgagg gtaggtataa ccaatattca ggataccttt ttcatcaaat
     8341 atttcttttt gcatccacca tcttatattc cgattgacta accccttgat ttcacctaat
     8401 ttaaaaggca aaagatcggc ccacaacata gctgaccaaa agcttgaaac tgcaaaacga
     8461 taaatcatgc ttcgaccaaa aggcacattt gcaccatctt ccgcaaaaaa atgaatatag
     8521 tctctagaaa aatcaatagc tcggttctta tatatctggc taatgggctt ttctggttct
     8581 agtacgctat aaatcaaacc ataataatga aatgcaaaag gattataata atcttggcga
     8641 cctcttgaac tgtctcgata ccagccattt tgaagataac aattttctat attatcaaat
     8701 gcttctttca gtgctgattc tttaactgga taaccaaatt tttggataat tgttccgaca
     8761 attatcttaa aaaattgcca attaccatct gcacattcat attcaaaaat ttgcgagaac
     8821 caatgaataa tctggctttt ttctcctgta tctaattttt tccataataa ttgttcatga
     8881 tgatataacg ctaataacat tgcgggcatt tctaccatac gctggtctct ttcatgtatt
     8941 tctccccaat aatcgggatg ggaaggatct gttccagctt ttattcctga tagaattaat
     9001 ttcaaccatc gttcatctaa ttggtcaatt gcgggccccg ctccccataa aacccgagag
     9061 aatccttcca ttcctgcaat tttatttcca tatcctaccc cttgatgtat ataatcaatt
     9121 tgtgcgccac ttgctcgata aaacggctgg agtggttgat ttatcttttt aaataagtca
     9181 aagtaatcct gtctggtcct taatagtggt actggatgtt tcattgtatt ttctcctctt
     9241 tttactacca aatagctaat tccgactgtt ctaatcgaag taagccttca ataaaaaaat
     9301 aatcagaata gatcagtggc acattataag tcagttctct atggtatgct tctgctccgc
     9361 ctccaatgat cccatcttca tccatattcc aatttgcata gtgcttttct gtcttttgga
     9421 gtatattgat agcagcttca tgatacattt ttgatcgtac agaggaaaca tatcctgcta
     9481 attccagaag accgcatgca gcgcatactc cagcactggt atcaatattc gaatctttta
     9541 cagcaaaatc aatgagagga atataaccag ttttttcgat tttctgaata aaatttactg
     9601 ctatcttttc tgctatagct agatagtacg gattttttgt gtaacgataa cttaacgcga
     9661 atccatataa agcccatgca tttcctctgc tccaacaaga attctcattg tatccttgcc
     9721 cgccaagtaa ccgaatatat ttccctgatt ctgcatcaaa ttctgcgata tgcccggttg
     9781 atccatcttt cctcactaga tacttagctg ttgtatctgc atgctgccga gcaattttag
     9841 agaacctcgg atcaccagtt tccctcgaag cccaatacag taatggtaaa ttcatcatac
     9901 tatcaataat catccaacca ggtttatggt cattccatgc tactaaatag ttccctgaaa
     9961 tattatagcg tccagcaagt accgtagctg catgtaaccc tagttgtcga ctatgtttgt
    10021 tttgagactg ttgataatgt gcaatagccg atggtaagaa taaaaaacct acatcatgat
    10081 gaacgtctga aaaatggtac agggcagatt ctaataattg ttcttgttgt attgctgcct
    10141 ctttgaactt ttctcgtttt gtcttgtgaa agagctgcca taaaatacca ctccaaaaac
    10201 cattggtcca ccagtcgatt cctctgtcct ttattacgtc tgtatatttt ccgttgtaag
    10261 aaatatatgg aattttattc cccattctgt ctaattccgc atctatcttt tcagaagcta
    10321 cattcaaaat ttttcttgac cattccttcg tctgttcatt catggatatc ccgccattct
    10381 tttaattttt atgaatgtgg agttgctcct agaacgaact agatggttaa cattcgttat
    10441 tttttttact tatttgcagc tccaatgtca ttctccttaa atttagactg cgcccacaca
    10501 taaatcaaga ccattgggat cactgaagtt gctaaaatta gcataattgc gccataatta
    10561 attggctcac ctgtactaga aaccgtcata tttttgataa ttaagggaac cgtatattta
    10621 tctttatctg aaagcatgac taatggaaat agatattgat tccaacttcc cataaagata
    10681 agtaaaccac tagtaataat cgtaggtgat aaaatcggta cagcgatttt cgtaaaaatc
    10741 acccactcat tggcaccatc gattctggca gcctccatca gtgctgtagg aaattgactg
    10801 ctatattgtc gcatcataaa cactgaagtt gtactggcaa tcgaagggat gatcacacct
    10861 gcataagtat tcgataatcc taaacgactc ataatgtaaa ataaaggaat caatgtggta
    10921 aaaaaaggca ccatcctaga tagcataact gtattgaaca acagtttatt ccccataaaa
    10981 cgatactttg ctaatgcata acccgccatt gtagaactgg ctgtagctaa aactgtactg
    11041 attacagcaa taacaatcga attccaaata atcaagggat aattatatct cgccatgatg
    11101 gcttgccagt tttttaatag catatcccca aacttgaaat tgattccgcc acttaaaatt
    11161 tcttgattac tttgcgttga agctacaatc atatataaat atggcatcaa aaatattaag
    11221 gcaccaaaca caataatgac gtacgtaaaa attctgcttt gtatcttttg tttagtcatt
    11281 tttatcacct ccgaatttaa attgaatagc cgataatata acagatacaa acaccatcgt
    11341 ccaggcaata gcagaggctt ttccaaaatc aaccatttca aaacttgttt tatataaatt
    11401 gactcctacg accaaagctg aattttctgg tccattggaa gtcccaaaga atatatttgg
    11461 gatattgaaa ttacccaagc cattgatagt ggctaaaact gtggccatta aaatgattgg
    11521 ctttagcatg gggatcgtta ataaaaagaa ttgccgaaat ttcgatgctc catctatttc
    11581 agcactttcg tatatctctt tagaaatccc ttgaagacca gctaaaaata aaatagtgtt
    11641 atatcctaca ctaagccaaa tcatcgtgat gatcattgat attcgtatcc aaaaaggatt
    11701 tgttaaccac tgtacaccgt taattcctag cgcattcaaa aatccattga ataacccagt
    11761 cggattgaaa aaagtcatga atacggaagc tacagccacc gttgatgtaa tggttggaat
    11821 ataatagaca gttctaaaaa atccttttcc ttttaactgt gctgaattca ataaaacagc
    11881 aaagtaaata gctaatagca tcgttattgg aattgatcct gccatgataa tcaaaacatt
    11941 cgtgtaagat ttaataaaca atggatctcg gaagatagct agataattgt caaatccaac
    12001 aaattcaaat gagtagccaa ttttcataaa actaaaagca aacgagataa tgatgggaat
    12061 caaaatcgtc acaataaaga aaataacaaa aggtgtgacc aatccataag cacttttttt
    12121 atgagacgta tctgttgctt ttttcttgct gttctttatt gtcttgcttg gtaacgaaga
    12181 ttgcatcatc gttaagtcct cctaatattt tttaatcacc agttaatttt acgccgtact
    12241 tatgagcaaa attttctgct tcttttagat atgaattttc aaaatcacct gtttgccaat
    12301 agttatatgc agcggtagtc aaataagacg tagcatctga ataagtggct ggtaaaacag
    12361 tatttgatgt attttcattg attttattca atagttcata gtatttttgt cctccttagt
    12421 agtcaaactt tttctcagct gcttctgttt gataggccaa tgagttagat gtagccacgc
    12481 ccagctccag tgcgttggct aagcattctt catctgtcag cataaatgta agaaattgtt
    12541 gtgccacttg tggatttttg gcatctttag ggacataaaa acttgatcct cctgatactg
    12601 caccttttcc tggattttct tttgaaaaag gaatcagtgg agccatgcgc catttgtcag
    12661 atgcatcagg aaaattcaaa gacatattcg tagcatacca gccgccagca acaaacattg
    12721 cagattcttg aaatgctttc tcagcatctt gtgcaccgta gaaatgtaca atatctgagt
    12781 cgatcatttc tttgattaac tttgcagcat gtttcgcttc tttagtattc agacggatat
    12841 ttccttcttc atcaagcaaa gattgattct gctgggctag catgttgata aacaattctt
    12901 ggtccccaga ttctggcaag gcaattccaa aactaccagt tttttctttt aatgcttttg
    12961 ccacctggat atattcttcc caagaactga tttcttcaaa atctacacct atttcagcaa
    13021 atttttttgg ttgataataa caaagtgctg gggtaaaatc actagcccaa ggaataatcg
    13081 tttcattact cgtttgttga cgcaaaatat tcaatttttg atcataaaac tgatcgccat
    13141 atttgtcata aaatccatat tcatcagcac tataaaaagc atcttgaaat ttttccagca
    13201 cacctgctgc tgaagtgtct gccatggaga taatatccgg catatcttcg tgtgttacca
    13261 attttggggt gatcatttgt actgttgctg ttcccgaagc tacattcaca atttttaagt
    13321 caatatttgc tccagttttg cggttataga tttttgcagc attttcaaca tagtttgtca
    13381 gtttgccgtt tgcgacatat acttctaatg tagtggcttt ttttatcatt ggatctgcga
    13441 tgtttgctgt tacttcttcg ttagtttttc cacatgcact taagctgaat aatagggtaa
    13501 tgtttagcaa agctatcacc aatttgtatc tttttttcat gtctatccct tctttctttt
    13561 caagttctta tataaaacgc tttcacttat gt
//
LOCUS       DAGCYL010000070        13406 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.071, whole genome shotgun sequence.
ACCESSION   DAGCYL010000070 DAGCYL010000000
VERSION     DAGCYL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.071"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            54..1952
                     /locus_tag="MB216_001501"
     CDS             54..1952
                     /locus_tag="MB216_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBT4526342.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(2282..3001)
                     /locus_tag="MB216_001502"
     CDS             complement(2282..3001)
                     /locus_tag="MB216_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBT4526343.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            3338..3670
                     /locus_tag="MB216_001503"
     CDS             3338..3670
                     /locus_tag="MB216_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526344.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3657..5660
                     /locus_tag="MB216_001504"
     CDS             3657..5660
                     /locus_tag="MB216_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBT4526345.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5679..6149
                     /locus_tag="MB216_001505"
     CDS             5679..6149
                     /locus_tag="MB216_001505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBT4526346.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6170..6754
                     /locus_tag="MB216_001506"
     CDS             6170..6754
                     /locus_tag="MB216_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBT4526347.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6769..7755
                     /locus_tag="MB216_001507"
     CDS             6769..7755
                     /locus_tag="MB216_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBT4526348.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            7745..8056
                     /locus_tag="MB216_001508"
     CDS             7745..8056
                     /locus_tag="MB216_001508"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBT4526349.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8078..9859
                     /locus_tag="MB216_001509"
     CDS             8078..9859
                     /locus_tag="MB216_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBT4526350.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9852..11228
                     /locus_tag="MB216_001510"
     CDS             9852..11228
                     /locus_tag="MB216_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBT4526351.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11235..11870
                     /locus_tag="MB216_001511"
     CDS             11235..11870
                     /locus_tag="MB216_001511"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBT4526352.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11900..13255
                     /locus_tag="MB216_001512"
     CDS             11900..13255
                     /locus_tag="MB216_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBT4526353.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT         4338 a         2337 c         2995 g         3736 t
ORIGIN      
        1 tagtgtataa caactttata atattgagta taccaatttt tggaggcgtt ttaatggacc
       61 gttatttaga tttactttca gaaaaattcc cctcaacgga ggatgtcatc acggaaatca
      121 tcaatctcga agcaattttg cagcttccaa aaggaacaga gttatttttg agtgatatac
      181 atggcgaatt tcctgctttt gatcatattc tgcgtatcgg ttcaggaaat cttaaagaaa
      241 agatcaaaga ccttttttct gaacaaatga cggaaacgga tatcagccaa ttcactattt
      301 ttacggctta tccagaatat gcattaacga ctgattggta caaagaacaa gataaatcgc
      361 agctgatcca tcatttgatc gatcttttac ggtttacgac agtcaaatat acccggtcaa
      421 aagttcgaaa aggattgccg aaagaatata gctatatcat tgaggaactc ttgtatatcg
      481 atgaccgtat caatggaaaa aaagactatg taaaaaaaat cattgaacaa cttgttttga
      541 tgaaggaaga agaaaaattt ttaacgaaac ttgcacagac gatccaaaaa tctgtcatcg
      601 atcatttgca tatcgttgga gatatttttg accgaggaac tcaagcagcc aaagtgatgg
      661 atcgaatcat ggatttttct tctgtagata tccaatgggg aaatcatgat attttgtgga
      721 ttggtgcgta ttgcggctct gaggcatgtt tactgacact tttacggatt gcagcacgct
      781 ataattattt atttgaatta gagaaagaat atgggttgaa tttacgtcca ttgttttctt
      841 ttgctcatca aacttatcag aagaatccag tatttgcgcc taaaaacagt aaaaatctat
      901 ccgaaagaga gcaagaagaa ttagaaaaaa tccatcaggc actttctatc cttcaattca
      961 aatcagaaca tcagttattg gatcgtcgac ctgaatttaa aatggacgat cgtaaattgc
     1021 tggaaaaaat cgactatgaa gcatcaacga ttgatttaga aggtcagctc tatgagttga
     1081 aaggaacttg ttttcaaacg atccagccag atgatccaaa aaaattcttg gaagaggaac
     1141 aaaaagtgat tgatagtttg atgtattcat tccagcactc tttgagaatg caaaaacata
     1201 tgacgttttt gatcgaaaaa gggggaatgt atctcacgaa caatcagcat gtgctgtttc
     1261 atggctgtat acctgtagac gagaagggac agctcttagc atttgaatta gaccaatctt
     1321 atcgcggaaa agaactgctg ggcttttttg aaaaacagat aactgaaagc gcaaaagatc
     1381 tgactgcaaa agaagattta gcaaccgatt tgatttggta tgcgtggagt gggccatttt
     1441 cgcctttatt cggcaagagt aaaatggcga cttttgaacg ttattttctc acagagcctc
     1501 atacgcacgt cgagaaaagc aatccttatt atcacttgcg agatgaagag tggttcagtg
     1561 aaaaaatttt agcagaattc gacacaaaag aagaagggtc agcaatcatt aatgggcaca
     1621 cgccagtaaa agtaaaaaaa ggggagtcac cgatcaaagc aaatggaaaa gtatttgtca
     1681 ttgacggagg tttatccaaa gcttatcaaa aaacgacagg aattgccggt tattcactat
     1741 tatgcaattc ttatggtttc caaatcgtga cacattatcc gtttttgcct attgaacaac
     1801 aatttgcaaa aaaatccgac caaacaaatg tgaaaaaagt aatcgaacag caactaccac
     1861 gcaaactcaa tcgtgataca acaaaaggta tggaactgca gcagcagatt acccagctca
     1921 aaaggctgtt ggactatcga aaggacgatt gagaagaatt ccagtgaaca ttgttccata
     1981 gactggagac tgagacaaaa gtcgtgtcgc agtctctttt tccggataaa cggtgttctt
     2041 ctgccaactc ctcggtcaca agtcacacta ttcaacaaac atgaaaagct gttttttgaa
     2101 attgctcctt gtgactcaga gttgaacggc aggctcacga cctcttggta taaacggtgt
     2161 tcaaaagcca gtttctcggt attaagccaa atttatgcaa aaatgtgaaa agccattttc
     2221 acaaaatttt tcttaatact cgaaactaaa cagctttttc acaaccccct cagaaagtct
     2281 attaaaattc attcacaaag aaatcgaata agcgctgttc agatggatgt acacggtggg
     2341 taagttccgg atgccattga acaccgagga ttttatgcat atcatctttg ctttgaaccg
     2401 cttcaacgag tccatcgtta gaaaaagcga tggcttttaa taaaggtgaa agatctttga
     2461 ctgcctgatg atggtaagaa ttcacaaaat atggatcggg gagaaggttg cttaacaaac
     2521 tgtctgaaac aatcttgact tcgtgagtcg taaattgcgg ggcagtgggc tgttgttcat
     2581 gttttactga ccactttgga tataatgaaa gatcttgata aagtgtacct tccaaaacga
     2641 cattcaatag ttggagtccc cggcagactg caaaaattgg ttttctttgt ttcaatgctt
     2701 ccaagatcag tgcctgttca aattggtctc gttgaatatt tgtttccgtt agctttggat
     2761 gaggatcttc catgtaaagt tgtggactga catcttgacc acctgcaaga ataagtttgt
     2821 cgatttgtcc aatcagttga ggagctgaat ctggagacat gactggcaga ataagaggca
     2881 atccttgagc atctaaaact gcatccacga atccttgcgg tgtatagctc acttgatttc
     2941 cttggaaagt atctgttgct cgaatcagtt gatttccggc aatgccgatg attggtttca
     3001 tttgtattcc ccctaaagta gacttattgt aacaaggatt taaaaaaaat taaactatgt
     3061 aagacttgcc cctatactta tctgttcttg ctgtgttact atctgtaaag agaaatttcg
     3121 tgtgaaattc atgacgatag ttgaaaatat tgcttaattt gttataataa aaagtaagca
     3181 gaaataaaaa ggttgggcgt aagcttcaag gatctagttc ttcaaggggt gagaaggaca
     3241 ttttattgaa ggggcctgcc tatactaagt tatctttaga ggtacatact ttcaatgaaa
     3301 aaagaaagca tgtacctctt ttttaaatcg aaaggaagtg aagcaagtgt tagaagcttt
     3361 ggaacaaatc cgaaaagcag aagaagaaaa tgaaaagcgt gctcaaacac tgcggcagca
     3421 gctgaagatc tatgcaacag aaaaagagca ggaattggaa acgatccaaa caaactatcg
     3481 acaacaacta aaacagttgg tggaagaaaa agaacaattt gaaatacaag aattagaaaa
     3541 agaaaaaatg aaattgaaag cagaagcaga caaggtgcaa gcaactttga aagatcaata
     3601 tcaaaagcat cagagtcaag cgatcggtgc gatcattgaa agggtgaaag aaacttatgg
     3661 ccgtcactaa gatggaaaaa gtgaccctca tttcagataa aagaaatcag gagaggattt
     3721 tacaagcagt acaaggaatc caaaacgttg aaatccggga tctgtatcaa aatagaacaa
     3781 acaaccagtg ggtggagaag tattttccag ataccacgat tattgataaa gaagcaaaat
     3841 taagcgcttt aggtaatcgg ttgaaagaca tccaagaagc tatccaattc atacaacacc
     3901 acggcgatag ccgtcaaaag aaactccatt tgaaacgtca agaactttcc ttacaagatt
     3961 tggaagccaa ttattccgaa gaaaatttca taaataaatt gaaagaagtc cttgatttaa
     4021 aagaaaagtg ggaacaacta actgaagaac gtgatcgttt agaagaagag gaagaatggc
     4081 ttcttcattg gcaacagctg gatattgctc caagtatgta tgcaagtaag acgaccattt
     4141 ttgagatggg gactgtgagt gcagcaaatt ttgaaagttt caaggaagaa ctgagtaagc
     4201 ttgatgacgt ttatatggaa gaaatagatt atacgccaaa acaagtttat ctttcgtatg
     4261 ttgctttaaa aaaatcagtt gctgttatag aagaaattgc tgggaaatac gggttcaata
     4321 aagaaacgta tccttatgaa caaaatccta aagaaaaact gcaagagacc aagcagcaat
     4381 tatccattgt tcacgagcag caaaagaaga taacgacagc gttaggtcag tgcagtggct
     4441 atatccaaga atttgaatgg gtagaagaag tcacacttgc cgttgcagaa cgagaaaaaa
     4501 taaaagaacg gtttatccat gcgacttatc tgatcgtttt gcaaggttgg gtagatgcag
     4561 aagaaaaaca gaatttgctg catgtgttaa acgaaaccgt atcagaagaa gatatatacg
     4621 tcagtttcga aacaccaaac tcagaagaga ttcagcagga agtccctact aaattgaaaa
     4681 accatccgct tgtcgaacct tttgaattgc tgactgaaat gtacagtctg ccaaaatatg
     4741 aagaagtaga tccaacacct tggatgacac cattttatct tgtcttcttc gggatgatgg
     4801 tcgcagatgt tggttatggt ttgctgatgc tcattgggac aatactagct caaaaactac
     4861 ttgttctacc acgcgggatg aaacgtttcg taaaattctt tgagattctc tcgataccat
     4921 cgatcatctg gggattgatc tacagttcat ttttcggaca gtcattacca aaagaactgt
     4981 ttggcatccg cttgcctttc ccaatcctat ctacgacgga tgacgtcaat acgattttga
     5041 ttttatccgt gattttcggt ctgatacaga ttttggttgg attatttgtg gcagcaaagg
     5101 aaaatatcag gagaaaagat tacttgagtg ctgtcagtga tggatttgcc tggcaaggga
     5161 tcctaattgg gatcgttatt gcgttggttg gtgccatgtt gataaaaaat acagcatttg
     5221 tttaccttgg cgctgggcta tcgatcattg ctgccttatg tatcttgatc gttccgatta
     5281 tccaaacgcc gtctaaagtc aaaggtgctg ccaaaggtgc gtataatctt tacggactga
     5341 ccggctatat cggtgattta gtcagttata cacgtttgat ggcattgggt atttcaggcg
     5401 gaagtattgg agcggcattc aatatgctag ttgctttcat gccgccagct gctcgattca
     5461 gtgtagggat ccttttgatc atcgctttac acgcactgaa tatgtttttg acactgctaa
     5521 gtgcctatgt ccatggcgca cgattgcagt atgtcgaatt ttttgggaag ttttatacag
     5581 gaggcggacg tgcctttgat ccgctgaaaa cggcggaaaa atacgtcaac atcaatcaca
     5641 agaaaaaaaa caataaataa ttggaggatt tttatataat gatggattac ttaattagtc
     5701 aaaatggtgg aatggtattt gcggtattag cgatggcaac agcgacgatt ttttcaggga
     5761 tcggttcagc taaaggtgtt ggaatgacag gtgaggcagc agcagcattg acaacaagtc
     5821 aacccgaaaa attcgggcag gctctgattc tgcaattgct tccaggcacg caaggtttgt
     5881 atggattcgt tattgccttt ttgattttta tcaacttagg aaatgacatg tcagttgtac
     5941 aaggactgaa ctttctaggt gcttctctgc cgattgcttt tacaggcttg ttttcaggta
     6001 ttgcccaagg aaaagtcgca gcggcaggta ttcaaatttt agcgaaaaaa cctgaacatg
     6061 ctacaaaagg aatcattttt gctgcgatgg ttgaaacata cgcgatttta ggtttcgtta
     6121 tttccttcct attagtgttg aatgcctaag cataaaggag gaaaagcaga tgaacgccat
     6181 tgaaaaaatc atctctcaga tgaacgaagc agcagagcag gaaagagcgg cgttagaaca
     6241 agaagagcga atgaagatcg atcaaaactt tgaacaaaaa cgtacgcaag ttgaaacaga
     6301 acatcaaaaa caaaaagaaa aacaaatcga attactcgaa aaaaaatacc gtcagctaag
     6361 gaatcgccag caagtagaag tacgacaaga aaatttgaat gcgaagcagg aatttttaag
     6421 acgattgttt gcagatgcgg taactgaaat ggaaaattgg gatgaatctg aacaaatcca
     6481 gtttatcaaa aatgcattgt attcattacc tttgacagga aaagtagcat tcattgctgg
     6541 agaaaaatca gcagcctatc tttctcagac gttgttggat gaatggaaca atgaacttcc
     6601 ctttatgatg gttttaagtg atgaaactgt tgctgatcaa gcgggttttc tgataaacga
     6661 tcaaggagtt cagtataatt ttcttttcag cagccttgta caagatatcc aaggaacgat
     6721 gagttttgag attgccaatc aattattcga ataaggaggg atcagtgtat ggaataccac
     6781 gaactaaatc ccttgattcg cggaagagag cttgaacttt tgtccaatga gacatttgaa
     6841 cggatgattc aaacaaattc tgtcgaagca cttggtgaaa tattgaaatc cacgatttac
     6901 agtccatata tttatgacgg atatgaatat gacttcgaac agaatctttt aaaggagcag
     6961 agccgtctat ttcgctggtt aaaggaatca gcaccggagc ctgaaatcgt ttggatctat
     7021 acgatgcgtt atactttcca taatctgaag gttttgacaa aagctgagat gacaaatcaa
     7081 aatctggacc atttgtatat cgacgatgga ttttattcat tagaaacctt gaaggatgcc
     7141 attcatacgc aagtttcaaa tgaacttcct gaaagcatta tggacagtat acgagaagta
     7201 cgtgattatt ttgaagagtc taccattctt cagggaattg acgtgattta tgatcgacag
     7261 tttttgacag agcagcgacg gcttgccgat aagctaggat atcaagaatt gctgacagaa
     7321 gtcattgctt ttattgactt gaccaatatc acaacgatgg ctcgaggaat attgcagaaa
     7381 agaacatatg ggttcatgac gacggtactt tccagctcgg gaagtatcga aaaagaagta
     7441 tttttgaact ttgtagataa agatatggaa gattacacga attttctatt agagacagat
     7501 tatgctgatt ttctgcgccc tgtcatccgt gagggaaata ttgatctcgt aaagcttgaa
     7561 cagttgaaag atgattatct aagttcattg tatcaagaag cacaaaccca ggctttcgga
     7621 ccactgccac tactagcctt tttgaacgca aaagaggtag aaagcaaaaa ccttcgttta
     7681 ctggttatcg gcaaaagaag ccatttcgaa tcagaaacaa tcagaaagcg ggtgagacaa
     7741 gtctatgacg cataagatcg gtgtagttgg cgacaaagat tctgttttgc cattcaaatt
     7801 gtttggcttt gatgttcgtt atggtacaac caagcaaacc gttcgtcagg caattgaaga
     7861 aatggcaaag aatgagtatg gcgtgattta tgtcacagag caatgtgcag cattgagccc
     7921 agaaacgatc gaacgttaca aggaacaaat gatacctgct atcgtattga ttcccaatca
     7981 tcaaggatca ttagggatag ggctggaaga aattcaaaaa aacgtagaaa aagcagtagg
     8041 tcaaaatatc ttatagtatg aaaaggagat agaaattttg caaattggaa aaatcataaa
     8101 agtctccgga cctcttgtaa tggctgagaa catgtctgat gccagtatcc aagacatgtg
     8161 tctagtggga gatctaggag ttatcggaga aattatcgag atgcgtggcg atgtggcgtc
     8221 tattcaagta tatgaagaga cttctggaat tggtccaggc gaaccggttc gctctactgg
     8281 tgaagcttta tctgttgaat taggaccagg gatcatttca caaatgtttg acggaatcca
     8341 acggccatta gataccttta tggaaatcac acaaagtaat ttcttagggc gcggagttca
     8401 attgcccgca ttggaccatg agaaaaaatg gtggttcgaa ccaacagtag aagccggaga
     8461 aacggtgagt gctggagatg ttatcgggat cgtagaagaa acaaaagtta taaaacataa
     8521 aatcatggtg ccaaatggga taaaaggaac gatcaaaaca atcgaaaaag gttcattcac
     8581 tattgatgaa acagtatgtg tcattgaaac cgaaaatggt gacaaaaatt tgacgatgct
     8641 tcaaaaatgg ccagtaagac gtagtcggcc aatcaaagaa aaattaaacc cggatgcacc
     8701 catgatcact ggacagcgag tgattgatac attcttccca gtgacaaaag gaggagcagc
     8761 agctgtacct ggtccttttg gtgcagggaa aacagtcgtt cagcatcaaa ttgcaaaatg
     8821 ggccgatgtg gacatggttg tctatgtggg atgtggcgag cgtggaaatg agatgacgga
     8881 tgttttaaat gaattccctg aattgattga ccctaataca ggagaatcgt tgatggaacg
     8941 tacggtattg attgctaata catcaaacat gccagtagca gctcgagaag cttctatcta
     9001 tacgggaatc acaattgcgg aatatttccg tgacatgggt tatgacgttg ctatcatggc
     9061 agactccact tcacgatggg cagaagcact tcgtgagatg agcggacgtt tggaagaaat
     9121 gcctggtgat gaaggttatc cagcttatct tggttcacgg cttgcggaat actatgaacg
     9181 atctggaaga gtcattgcat taggatcaga aggtcgcgaa ggaagtataa ccgcaatcag
     9241 tgctgtatcc ccgtctggag gggatatttc ggaaccagtc acacaaaata cattgcgggt
     9301 cgtaaaagta ttctggggtc ttgattccag tttagcccaa aaaagacatt ttccttcgat
     9361 cgattggata caaagctatt ctctttattc aacagaagta gggaagtata tggatcatat
     9421 ccttcaagct gactgggcat ccatggtcac cgagggcatg cgtattttgc aggaagaaca
     9481 gcagctgaat gaaatcgtgc gtttggtagg gattgattcc ctttcagata atgaccgtct
     9541 gacattagaa gtggcaaaat ccattcgtga agattatttg cagcaaaatg cttttgatga
     9601 tgtcgataca ttcacttcaa gagagaaaca attcaaaatg ttgaagatga tcttgacctt
     9661 tggtgaagaa ggacgccgtg cgttgtcgtt aggtgcttat ttaagtgaga tcatggacgg
     9721 taccgtatcg attcgtgaaa ggatcggacg aagcaaatat atcccggaaa cagaattaga
     9781 aaaactagat caaattattg gtgaaatcaa gcaaacgatg caaacgatcg tttcagaagg
     9841 aggcatgagc aatgattaaa gaatatcgta cgatcggtga agtcgtcggt ccattgatgg
     9901 cggttgaaaa agttgctggt gtcaaatatg aagaattaat cgaagtccgt atgcaaaatg
     9961 gcgagattcg tcgtggacaa gtattggaag tccaagaaga taaagcaatg gtccaaatat
    10021 tcgaaggaac aagtggtatc aatttacgtg actcttctgt acgattttta ggccatcctt
    10081 tagaactagg tgtatcggaa gacatgatcg gtcgagtatt cgatggtttg ggacgaccga
    10141 aagataacgg accagagatc ctgcctgaaa agcatttgga tattaacgga gaagtaatca
    10201 atccagtagc tcgggattat ccagatgagt ttatccaaac aggtatctct gcaatcgatc
    10261 atttgaatac attagttcgt gggcaaaaat taccagtatt ttctggctct ggtcttcctc
    10321 ataaagaatt agctgctcag atcgcgcgac aagcaacggt actcgattca tcagatgatt
    10381 tcgccgtagt ctttgctgct attggaatca cttttgaaga agctgaattt ttcatggaag
    10441 attttcgtca aacgggcgca atcgaccgtt ctgtcatgtt tatgaattta gcaaacgacc
    10501 ctgcaatcga acgtatcgct actcctcgta tggctttgac tgctgcagaa tatcttgctt
    10561 atgaaaaggg aatgcatgtt ttggttatca tgacagacat gaccaactat gcagaggcat
    10621 tgcgagagat ttctgcagca cggcgagaag ttccaggaag aagaggatat cctgggtatc
    10681 tttacacgaa cttagctact ttattcgaac gagcaggaag aatccgcgga ctaaaaggat
    10741 cggttactca aattccgatt ctaacaatgc cagaagacga taaaactcat ccaatccctg
    10801 acttgactgg ttatattaca gaaggacaga tcatcttgac tagagagctt tacaagagtg
    10861 ggattcagcc accaattgat gtactcccgt ctctttcgcg gttaaaagat aagggaactg
    10921 gacccggaaa gacaagagaa gaccatgcag ctacgatgaa tcagcttttt gcagcctatg
    10981 cacaaggaaa acaggcaaaa gaattagccg tagttttagg tgaatctgct ttatctgatg
    11041 tagacaagat ctatgcgaag tttgctgagc ggtttgaaaa agaatatgtc aatcaaggat
    11101 tctacacgaa ccgttcgatt actgaaacac ttgatctagg gtgggaactg ctatcgatgt
    11161 tgccaagaac cgaattaaaa cgaatcaaag acgacatgct ggacaaatat ctgccagaag
    11221 ggaagtgagt tctgatggca cgattgaatg tgaatccgac ccgcatggaa ttgactcgat
    11281 tgaaaaaaca gttgacaact gccacaagag ggcataaatt attaaaagat aagcaagatg
    11341 aactgatgcg tcaattcatc ctgttgattc gaaagaacaa ccagctgcgt caagaaatgg
    11401 aagagtctat gacagaagca atgtctgatt ttgtattggc aaatgcttcg atcacggaac
    11461 ctttcatcga agaattattt gttttgccgg cggaaaacgt tgatttgtct atcgtagaca
    11521 aaaatatcat gagtgtaaaa gtacctgtga tgaattttga atatgatgaa cagctgacag
    11581 acacaccttt ggaatacggg tatttgaatt ccagtgccga actggatcgg tcaatcgatc
    11641 ggtttactac cctgcttcca aaacttttgg aactgacaga aatcgaaaaa acttgtcaac
    11701 tgatggcgag tgaaatcgaa aagacacgtc ggcgagtcaa tgcattagaa tatatgacga
    11761 tccctcaatt agaagaaacg atctattata ttcgtatgaa gctggaagaa aacgagcgcg
    11821 cagaagtaac aaggttgatc aaagtaaaaa atatgggtac cactgactaa gtggtatctt
    11881 tttttaaaag aggaaaacga tgattaaaca aagcaaacaa ataaagaaac gtttgccggc
    11941 tgttcagctt ctcgcattag gattttttct cctgatttta gttggcggcg gcctacttac
    12001 cctaccattt tttagtaata gtgggcatgg gacaaatttt atcgatgcgc ttttcactgc
    12061 aacttctgct gtctgtgtaa cgggattgac aacattgaat acagcagagc attggaacag
    12121 tgctggtcaa tttttgatca tgcttttgat tgagatcggc ggtcttggtt ttatgatggt
    12181 tccgattcta tgctttgcat tgataaagaa gaaagtcagt ctcagcacac gtattatatt
    12241 aaaagaagcg ctgaatctag aagaagtatc aggtgtgatg aagctcatgc tttacatcct
    12301 aaaattggcc tttgtgatcc aattgattgg tacggttgct ttatcttttg tatttgtgcc
    12361 tgaatttggc tggttgaaag gtatctggta cagtttgttc catgcaatct ctagtttttg
    12421 taatgcaggg tttgatttgt taggagacag tttagccaat caccagcaaa atgtttattt
    12481 gatcatggtg gtatctgctt tgatcatttc tggtgggtta ggcttcatcg tatggcgtga
    12541 tttactggaa tatcatcaga aaaagaagat gacgattcat tcaaaaatcg cattaaccgt
    12601 tacaggtacc cttttattag gcggattcgt tgtgttcttt ttcacagagc gcaatggggc
    12661 aacgctagtg gaagggaata tgtttcaacg attggcaaac acctttttta tgagtgtgac
    12721 acctagaacg gctggatatt attcagttga ttacctccat atgagccatg caggtctgat
    12781 tttaacgatg tttttgatgt acatcggcgg aacatctggg tcgacggccg gcggattgaa
    12841 aacaactacg ttaggtgtgt tgctgatcca gatgcgttcg atgttgaaag gacgtacgag
    12901 agcggaagca tttggccgta ccatccgaca aggcgcagtg ttgcgggcac ttactttatt
    12961 ttttgttacg ttgacgttgt gtgtagtcgc catcatgatt ttatcggtga ctgaaacgat
    13021 acctaaaact tctggcatcg aatatattgc ttttgaggtc ttctctgcgt ttggtacagt
    13081 aggtcttacg atgggattga cgccagactt gactgcaata gggaaattga tcattatagc
    13141 gcttatgtac attggacgag ttggtgtatt gactgtggtc ttctctctga tgacaaaagc
    13201 acaaagaaca caatcaaaat acaaatatcc agaagaaagt gtcatgattg gataatttca
    13261 tcttctgaca gaaacctagt gatttagtaa aataagaaaa taaaataatt tttcttgctt
    13321 cttggatact ttaagtgcaa aatgttcgtt tattttttga ggcactaccc gttgttgaac
    13381 ctcgggtagt gcctcttttt tattga
//
LOCUS       DAGCYL010000071        13382 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.072, whole genome shotgun sequence.
ACCESSION   DAGCYL010000071 DAGCYL010000000
VERSION     DAGCYL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.072"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(162..527)
                     /locus_tag="MB216_001513"
     CDS             complement(162..527)
                     /locus_tag="MB216_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526354.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            735..1217
                     /locus_tag="MB216_001514"
     CDS             735..1217
                     /locus_tag="MB216_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526355.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1414..2304
                     /locus_tag="MB216_001515"
     CDS             1414..2304
                     /locus_tag="MB216_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBT4526356.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(2591..3865)
                     /locus_tag="MB216_001516"
     CDS             complement(2591..3865)
                     /locus_tag="MB216_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4526357.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            4138..6609
                     /locus_tag="MB216_001517"
     CDS             4138..6609
                     /locus_tag="MB216_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBT4526358.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(6712..8448)
                     /locus_tag="MB216_001518"
     CDS             complement(6712..8448)
                     /locus_tag="MB216_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBT4526359.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(8445..9149)
                     /locus_tag="MB216_001519"
     CDS             complement(8445..9149)
                     /locus_tag="MB216_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4526360.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            9280..9783
                     /locus_tag="MB216_001520"
     CDS             9280..9783
                     /locus_tag="MB216_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBT4526361.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            9743..10081
                     /locus_tag="MB216_001521"
     CDS             9743..10081
                     /locus_tag="MB216_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBT4526362.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10102..11520
                     /gene="ffh"
                     /locus_tag="MB216_001522"
     CDS             10102..11520
                     /gene="ffh"
                     /locus_tag="MB216_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBT4526363.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            11632..12210
                     /locus_tag="MB216_001523"
     CDS             11632..12210
                     /locus_tag="MB216_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBT4526364.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            12214..12735
                     /locus_tag="MB216_001524"
     CDS             12214..12735
                     /locus_tag="MB216_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBT4526365.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(12814..13368)
                     /locus_tag="MB216_001525"
     CDS             complement(12814..13368)
                     /locus_tag="MB216_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBT4526366.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         4330 a         2422 c         2763 g         3867 t
ORIGIN      
        1 gaatcattat tgctgcaatt attacacaac tgtttttagt gtttggagag aagaaaagaa
       61 gtaaaaaata gaaaaactag tgatagtttt ggtgatataa gaaaaacata tatgtataaa
      121 aagaagaaag tagcttctct ttgtacatat atggtttgtt tttatggatg ccgagctttt
      181 ttataagata aggctaccgc ccataacgac ccaaggattt gtggaactaa gtaaatacct
      241 gcaagaagca agatcagctg ggtcggtttt aaaggatgat agaagtaacg ccattcagta
      301 aaattggcgc aaaataaata ggaagtgaca gtagatataa tattggcaat gaataaagat
      361 ataatacgcc tgttcctttg aaaataatat ccagcaaaaa gagataaaaa tatcaatgtg
      421 atgaacccgc ttacggaatg ataacgaaaa tcaagcagta aggcgaaatg cacataagga
      481 acaaaagata aaagcaacgg aaggtttttc cagcaagttt ttatcatctt tcatcaccac
      541 ctaacaagtg ttattatttt gttagtttta ttgtaaattg aatcctgcaa taatcaatta
      601 ctttttcatt atttctagat taaatgaata aactctcatg ccattatctc taaaaaaaaa
      661 gtttttcccc ctaatttttt tagatagaaa attgtatagt atgatgagag aaaaagtagt
      721 ttgggggaac gttaatgaga agaaaagcag taatcgatcg gctttattta tttttttggt
      781 tttttcctat cgttttgcta ccttttacat gggtaaatgc tgttgtacaa ggtgcggaga
      841 tattaggcct gatccaagga agcggatttg tggtttctag gaattttcca gctttgattg
      901 ttttctattt agtgataagt gtaggtctct tattaccaaa atctctgatc aatcactaca
      961 attggcagct gctttacttt tcttgcttat tagtatttac atctatgttt tctttgctgc
     1021 ctcgcttgat tatcggccct aatggtttga gcgagagtgg attgaaggat ttagcttatt
     1081 cctccatgta tttcttgacg atcaaaccat ggttctattt agtaattgcc tcatttattc
     1141 ttgcaacatt tttttacttc tggacagaaa tcaggataaa aagtgaacga tctgtatcta
     1201 taaaaaataa ctattgatgg tggatcatca gtaaaaaaac atatgagctg cgtaaaatgc
     1261 atgtttctca tatgcttttt ttattattat gtaaagaatg cgaaaaacgt ttacatgatt
     1321 tttacataac ttaactatat ttttacagca accatacgag ggatttacag tcgactgata
     1381 aactttagtt gtaaataaga aaggagcaat aaaatgaaaa aagtattgtt tactctaggt
     1441 atgatgggaa ttcttttagc cggatgcggc tcaggatcag gaaatacaga tggctctgcc
     1501 acacaaaata attcaagtga ctcaaatgaa caagtaaaaa tcgttgcggt aggttcgaca
     1561 gcattacagc cgttagtcga tgcagcacaa gaaagctttg tacaagaaaa tccaaattat
     1621 caaatctctg tacaaggagg aggaagcgga actggtttga gccaagtcga agctggagca
     1681 gttactatcg gaaactctga tgtatttgca gaagaaagag acggagttga tgcttcaaaa
     1741 ttagtggatc ataaagtagc agtggtcggg atggcaccaa tcgtcaataa agatacagat
     1801 gtcaaagata tcaccaaaca ggaattgatt gatatcttta caggaaaaat cactaactgg
     1861 aaggaagttg gcgggaaaga tcaaaaaatc aacgtcgtga accgtgcaaa cggcagcggt
     1921 acccgcgcga cattcgaaaa atggggactg gatggtgcta cccctgtgca gtcccaagaa
     1981 caagattcat caggaacagt tcggcagctg gtaagtcaaa caccaggagc aatcagctat
     2041 ttagctttct catatctcga tgattccaca caagcactaa gtatcgacgg tgtagaacca
     2101 aaagaagaga atgtagcaga taacagctgg ggaatctggt catatgaaca tatgtacacg
     2161 aacggaaaac cttcccctga agtccaaaaa ttcttagact acatgatgac agaagaaatc
     2221 caagaaggac ctgtcaaaga attgggctat ttaccaatca caatgatgga agtagaacgt
     2281 gatcacgaag gaaatatcaa ataactagca gataaacatg ccacggtagt cgttactatt
     2341 aaggaaaaat caactatgtt tccccacata tacatttttc ctttaattgt gaaaaatagt
     2401 tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta
     2461 ttcgaaaaaa agagctacga catagaactg tcgtaacttt ttttggctct tcgtcaaatc
     2521 gtgttggtga agacaaattt gaataaaact tagtccgata tgcggcgcgt tctgtgcgcc
     2581 gcatattttt ttagttttca aagatcagac cttgttgtaa atagatccgt gcccgaaagt
     2641 tataaaagtt gcgataacca aacgcaattc gtttaatcag cttgatcttg ttattcagcc
     2701 cctcagtgac gccgtttgag tgggacacct taaaggcatt tgaaataccc gattggtagc
     2761 aatggaagat cgtgaatttc tttttaaacg actgaggtaa cgtagatgaa attgttttaa
     2821 gggtttcttg aaagccttcc gtacctcgtt taacgaaata atagcgtaat aattgacaaa
     2881 aatgatacgc agttcgcaat tcttcgttgt agcttaacag ctcatcaacg atttctgttt
     2941 gactaattgg tcgtttaaac agtcgatgat aatgccgttt gagtggctcc catgtcgtgg
     3001 aatctttgag taagagtttc caatatcgct tgagccgacg gtacttcttc tgattttcag
     3061 agtgtgaggt tcgaaaacga ttcatgatct ggacacgtaa ttgattgaag ctgcgattga
     3121 tgtgttggac aatatgaaag cggtcagtta cgatctccgc atttggaaag actgttttaa
     3181 gcaactgaca atagctggca ttcatgtcca tcactaggta cttgaccttt aaacgggcct
     3241 ttctaggaaa gcgcaggaag tattgagtta gtttgtaaag tctgcgatct tcaaggatcg
     3301 taaatagctg attggttgtc ccatcaacac aaataaaact catggaacca gaaacagaat
     3361 tcattgattt gaactcatcc atacacaaca ccgtcggaag gaagttcata cgtggctggt
     3421 aatgcttcgt tgcttcacgc aagatccgtg tcaccgtccc atccgagacg aagtacttct
     3481 tagcgattag tttacgtgat tgattttcgt acagttccat gagaatcgcc tgcttcagtt
     3541 cctttgttag ttgatgatgt tcatcaacta agtccgtttt cgcagaaaag gtccttccac
     3601 aatcatggca gagaaaacgg gtacgtttta attctaaaat agttaacttt tctttgactt
     3661 tcaacatctg agtgcgtgtc gtataggaac cgttgcggat aatacgaacc gatcgacagt
     3721 gttcacaata ggaagaagag tcatccaatt ttcctttaat aaactgcgct tgaacgcctt
     3781 tatattcttt ttcaatcagc cattcttcat caaaaaataa atgtttatct gttaatccga
     3841 gtaattttct agtatgatta tccattgaga gactcctttg gtaggatgac gttttggtcg
     3901 actaaatttt accaaaagtg agtctctttt tgagttgaaa cgaagaaaaa gtgttagtag
     3961 aaaatcgcct tcgattttcc accaacacaa aatattatag aacccttttt ttttgctata
     4021 tgacaggaat gaaaggtgtt tgcaagctta atgtgaggaa cactttccat atctgttcta
     4081 tactggatag gagaagtttg gaaatatcca gaaaacgaaa tcaatcgagg tagccacatg
     4141 aagaataaat ggataaatcg aagtaaaaaa aagaaagtaa cacaaagatc atcagataaa
     4201 gtcatccgtc tttttccatc gtataaagaa ggattaacgg cagatcaagt caaggaacga
     4261 atagaaaaag gtgcagcaaa taattcagtc gatcccacat tcaaaacaaa tcagcaaatc
     4321 gtattggaaa acatatttac gtatttcaat ttgatctttc ttatcttagc cattttgctt
     4381 tgtttagtag aatcctataa gaacttgact tttttaccag ttatcatagc caatacagga
     4441 atcgccattt ttcaggaaat tcgatccaaa aagatcttgg acgaactaaa tgttttaaat
     4501 acagcaaaag tcaatgtcat acgagacggg atagaacaaa caatagatat agaagacctt
     4561 gtaatagatg acattgttat tttagagact ggatatcaaa ttccagcaga tgcagaggtc
     4621 gtggaaggga aactgcaagt taatgaggct ttattgacag gcgaagcaga tgaaataacc
     4681 aaagaaatag gtgatccatt aatgtccggc agtttcattg tttccggaaa agcttacgcc
     4741 cgattagaca aagttggagc agattcgtat atttcccagc tgacagtcaa agccaagaca
     4801 atgggagaag gagaacagtc ggagatgatt gcttctctta atcaattgat caaatggatt
     4861 gggatcatca ttattcctat cggtatcctg cttttttctc aaagttactt ttttaatgga
     4921 aacacgatca aagaaagtgt cgttgcaatg gaagcagcat tgatcggtat gatacctgaa
     4981 ggactgtact tgctgacgac gattgcatta gcattaagtg cgactaaact agcaaagcaa
     5041 agagttcttt tacacaatat gaaaagcatt gagacattgg caagggtaaa tgtcctttgt
     5101 gtagataaaa caggaacgat cacagaaaat aaaatgtctg ttcaaaaaat gatcgtctct
     5161 aaaaaacaag aaataatcag tcagtcagat caattggaag catgcattgc tgactatgca
     5221 aaagcgatgg caggagacaa tgcaacaatg gaagctgtta aagcatattt tacaaaaacc
     5281 actgatcaat cctatcacca tgttattcct ttttcttctg tccaaaaatt cagcagtgta
     5341 gcgttggcag acaaggtgta tgttttagga gcgcccgaaa tggttttgcg cgatcaattg
     5401 ccggaatatg cggaagagtt tgtttctttc gctgaaaaag gctatcgcgt gttggtcttt
     5461 ggtgtgtacg atgggattct tgaagaacca gtattgacag aagcagtcac accattaggc
     5521 tatattctga ttgctaatcc tatcagaaaa gaagcaagag ccacatttga ttacttcaaa
     5581 aaacagcaag tagccatcaa agtgatttct ggagataatc cgctcaccgt atccaatgta
     5641 gctgtccaag cagggatttc acatgctgat cagtacgttg atgcatctaa attatcagaa
     5701 gaagaatatg aagcagctgt cgaaaaatat acagtttttg gacgcgtcaa gccagaacaa
     5761 aagaaaatct tcgttcaatt gataaagcgt aacaatacag tagctatgac aggtgatggc
     5821 gtgaatgata ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat
     5881 gaagccgcca tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca
     5941 gaagttgttg cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt
     6001 ttagtgaaga atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc
     6061 tatcctttag aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct
     6121 ttcttgcttg ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg
     6181 atggaaaaag ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc
     6241 tgcggggtga ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg
     6301 attgcagtag ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt
     6361 cagatcattc ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg
     6421 ttttcaatca ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg
     6481 gcagattcga tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat
     6541 gaaaaagagc aaaagccgtt tagtcgcgta gctaaaaaat ttctatcgat atttaaacga
     6601 gaaaattgac gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt
     6661 cgaagcagga tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt
     6721 cggaatggta acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc
     6781 attaaggagt tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg
     6841 tcggcttcgc gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat
     6901 gtcgatacct ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg
     6961 gataataatc tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat
     7021 aattggataa aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat
     7081 cgtcaaccgt ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg
     7141 ttctgtataa attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat
     7201 ttcccgaatc agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt
     7261 cagagtttgc gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga
     7321 agtcacagga gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc
     7381 taaacgtcgc actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga
     7441 attagtaaag tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc
     7501 ctgatggatc agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc
     7561 ttgaatgact tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg
     7621 catctttgga ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat
     7681 catcagttcg ttcagtaacg tatataattg ctcttctgtg gtagtacacg cccgatacat
     7741 cttactcatt tgttcgctca acgcattaat cgtctgatac aattcttccc attgatcggg
     7801 tgtttgcatg attacttccg cctgattagg attttgcacc atttttttta gtacgggcag
     7861 cacggttttc aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac
     7921 aaggaagaaa gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa
     7981 aaaaacgctt gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac
     8041 tggtaatgcg acatataaaa gatcctcatt caaagtagta cttttgcgca aagcgctacc
     8101 taggttccca ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa
     8161 tgtttcattg tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc
     8221 gttcgactga tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc
     8281 gacatctaat tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc
     8341 cacgatctgc tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac
     8401 taaaagaata agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc
     8461 tgaaacttat agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc
     8521 tcgatttttt ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag
     8581 tcaaaattcc aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc
     8641 atgaaataaa caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg
     8701 gtcacttgat aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca
     8761 gcttcatctt ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct
     8821 aatacttcgc gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca
     8881 atgatccgat ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt
     8941 tcttgacgca gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa
     9001 atgatgaaat caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca
     9061 ctggttacct tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt
     9121 tcatcatcaa caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt
     9181 ttatatttta gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa
     9241 taggaaggca ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt
     9301 ttaggcggtc tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa
     9361 ctagttgaaa ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt
     9421 cctagagatc ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca
     9481 aaagtatgga cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa
     9541 gcctatcaaa aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct
     9601 tcatgcggaa cccattcgat ttatgacgga agtttttccg gaataaaaaa aaatggtgcc
     9661 ggagtggcta ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg
     9721 ctgaaaacta gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt
     9781 tgaattttat tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc
     9841 agatgatttt tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata
     9901 tgacaatatc aagcggacaa gcaagatctt ggaggattat gaaaaaaaac tccatctttt
     9961 ttctaattat attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa
    10021 gtacccggaa gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata
    10081 aataaaagga atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg
    10141 atgggaaaaa taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg
    10201 gaaatccgcc ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact
    10261 aagcgcgtac gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa
    10321 caaatcgtaa aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag
    10381 ttaaataaat caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt
    10441 aaaacgacct ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg
    10501 ctcctaatcg caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt
    10561 caacagctag acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc
    10621 cgtcaaggga tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca
    10681 ggacgtctgc atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg
    10741 caaccaaatg agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta
    10801 gctgatagct tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg
    10861 gatactcgag gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc
    10921 atcggttctg gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc
    10981 cgtatccttg gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactacgac
    11041 gagaaaaaag cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac
    11101 tttattgaac agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg
    11161 atcccaggaa tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt
    11221 gaacgcaaaa aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg
    11281 ttgaacccaa gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg
    11341 aatcgaatga tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg
    11401 gatatgaaca tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc
    11461 aaaatggcga tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa
    11521 gatgaaaaaa acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag
    11581 caatttttat tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt
    11641 ccgtggagtg aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct
    11701 gaatatgatg accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg
    11761 agctggcaga cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat
    11821 atcaaaaaag tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga
    11881 attattcgta accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa
    11941 attcaagaag aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc
    12001 gttgattctt cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa
    12061 ttagcaaaag atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct
    12121 tttttagaag ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag
    12181 gtcgtacgta tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct
    12241 ataagtttag atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact
    12301 ccaacaggta agcaaactac ttatgaagct ttttatgaaa caatcgatac gaccatcatg
    12361 ggcagaaaaa cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag
    12421 aaaaagaatt tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta
    12481 aacgaagatc ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg
    12541 gttgtgggtg gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg
    12601 tatatccaga ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca
    12661 tatgaagcgc gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat
    12721 tatgtacgaa agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt
    12781 acatagttgc cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc
    12841 tagatacgca tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt
    12901 ttcatcaatt agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac
    12961 attcaaaaag acaagtgatt ggcgtagatg gtggtttgcg ccaaagccgc cgatcccacc
    13021 tggtgatgca gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct
    13081 tgagcctaca tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt
    13141 aacgaaaacg acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg
    13201 agcctgttct ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa
    13261 tacactttcg tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta
    13321 agaatctttt cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct
    13381 cc
//
LOCUS       DAGCYL010000072        13338 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.073, whole genome shotgun sequence.
ACCESSION   DAGCYL010000072 DAGCYL010000000
VERSION     DAGCYL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.073"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(624..1307)
                     /locus_tag="MB216_001526"
     CDS             complement(624..1307)
                     /locus_tag="MB216_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBT4526367.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(1325..1942)
                     /locus_tag="MB216_001527"
     CDS             complement(1325..1942)
                     /locus_tag="MB216_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBT4526368.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(1952..2776)
                     /locus_tag="MB216_001528"
     CDS             complement(1952..2776)
                     /locus_tag="MB216_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBT4526369.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(2769..3329)
                     /locus_tag="MB216_001529"
     CDS             complement(2769..3329)
                     /locus_tag="MB216_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526370.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            3502..4458
                     /locus_tag="MB216_001530"
     CDS             3502..4458
                     /locus_tag="MB216_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4526371.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(4763..5719)
                     /locus_tag="MB216_001531"
     CDS             complement(4763..5719)
                     /locus_tag="MB216_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBT4526372.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            5823..6425
                     /locus_tag="MB216_001532"
     CDS             5823..6425
                     /locus_tag="MB216_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBT4526373.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(6513..8012)
                     /locus_tag="MB216_001533"
     CDS             complement(6513..8012)
                     /locus_tag="MB216_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBT4526374.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(8028..8552)
                     /locus_tag="MB216_001534"
     CDS             complement(8028..8552)
                     /locus_tag="MB216_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBT4526375.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            8850..9599
                     /locus_tag="MB216_001535"
     CDS             8850..9599
                     /locus_tag="MB216_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBT4526376.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9590..10375
                     /locus_tag="MB216_001536"
     CDS             9590..10375
                     /locus_tag="MB216_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBT4526377.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            10344..11060
                     /locus_tag="MB216_001537"
     CDS             10344..11060
                     /locus_tag="MB216_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBT4526378.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(11162..12196)
                     /locus_tag="MB216_001538"
     CDS             complement(11162..12196)
                     /locus_tag="MB216_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBT4526379.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(12200..13258)
                     /locus_tag="MB216_001539"
     CDS             complement(12200..13258)
                     /locus_tag="MB216_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBT4526380.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         3908 a         2757 c         2395 g         4278 t
ORIGIN      
        1 atttattaaa cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc
       61 taggatgaac aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg
      121 ctaaaaacac ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca
      181 gacaactgcc caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc
      241 cctaaaaaac agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg
      301 atgcacaagc attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc
      361 gacaatttag ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt
      421 ctatagaaag cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa
      481 aggttttaac tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt
      541 tgaattctga acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt
      601 cctcgtcttt tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt
      661 tccattttac gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct
      721 cctgcttgaa tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga
      781 agttcaggat attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg
      841 atattgataa tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt
      901 accgtactga taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc
      961 gtaggaccgt tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag
     1021 gaagaacgag caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct
     1081 gcctcaataa tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact
     1141 gcattcgcat ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc
     1201 aattttccaa atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata
     1261 tcttttctca attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc
     1321 tttgctatct ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt
     1381 gcgcataacc ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat
     1441 agttggtggc cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga
     1501 acagttgtgt atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt
     1561 ggatagttat ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat
     1621 ccccataagc cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc
     1681 aatttgattc taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt
     1741 gtagttcttg ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat
     1801 tttcttgctg attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta
     1861 agagtatcac tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct
     1921 ttttccactt ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt
     1981 cttctagcgt caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta
     2041 agtgccaagg ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga
     2101 taaatccata ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac
     2161 ttgcatccaa tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg
     2221 ccagacctgt gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg
     2281 tcgcctcctc ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa
     2341 cttgttgttg ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg
     2401 cttcagcagc cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc
     2461 cgttacttaa tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg
     2521 gaccaaccaa aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac
     2581 gatcttttgt ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac
     2641 tagcagttgt tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat
     2701 catcatcgct aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc
     2761 ctaaaaattt attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca
     2821 tgaacgtaag tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct
     2881 tcgatctgtt tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat
     2941 tcatcatatt gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc
     3001 aaagggatca gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt
     3061 ttgatttgct tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga
     3121 cgcaaaatct tttgcgttag catccattca taatcagtga aaagattttt tactttctgc
     3181 atcgcttcag atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg
     3241 atcagcaagt attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc
     3301 ataataagat caatatatac ttgatgcacg tttatcccct ccttttaact attctactta
     3361 agttttgatc aaaaagcacc tcttaaatcg ctgctttcat ttttttaatg tttcaagtcc
     3421 tctcctcttt attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca
     3481 actatgaagg agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc
     3541 atcgcacatg agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct
     3601 tccagaactt cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac
     3661 ggaagctatc aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca
     3721 aatagacagc atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt
     3781 gaaaaagcca tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa
     3841 aagaatttga tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta
     3901 cgctcaataa tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc
     3961 agttacaaag aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct
     4021 ctgttggata tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct
     4081 gaagtagttg cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact
     4141 atcttgcgta acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg
     4201 ccaaaagtcg gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt
     4261 gctgatcgtg cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat
     4321 acatctgaag caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg
     4381 cccaatcgct ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt
     4441 ctatggaaaa atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa
     4501 gaacagccaa acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga
     4561 ggaaagcttt tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc
     4621 acaaggagca atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg
     4681 aggagttgac aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga
     4741 atattagctg aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga
     4801 tttcaataaa acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt
     4861 cagggcgact agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg
     4921 ttaaaatttg tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac
     4981 catagatatg ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt
     5041 ctttaatcac attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct
     5101 gtctgagcca gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg
     5161 tcagtaaatg ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg
     5221 ccatttgtct caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct
     5281 gcttcgtacg atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca
     5341 ttaatcccgc ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag
     5401 tgaccaaaac cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga
     5461 tgaacaggaa taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga
     5521 acagtgctgt tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg
     5581 tagagcgctt ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca
     5641 acagcgataa gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg
     5701 ttcgcattcc tatacgcatt tacttccccc ttttttattt taattatact tgaataatga
     5761 aaaactagct atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct
     5821 ctatgcaatt tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt
     5881 ttttatgtat cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat
     5941 ctgctgtttt ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga
     6001 tcatcggcaa tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt
     6061 ttcacaatga ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg
     6121 tcgtgtcaga tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc
     6181 tgattgcgtt gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag
     6241 atacttttat agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa
     6301 cagaaaagaa ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc
     6361 taagaacaaa tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg
     6421 actaattttc ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct
     6481 ccatttttga ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct
     6541 gcctcttttg gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc
     6601 tctacgattt ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa
     6661 agcaaatttg tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt
     6721 cctgtatcga caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc
     6781 aattgcggta gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag
     6841 gcattggcga tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg
     6901 gcttctgctt gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca
     6961 cggaatcgtt ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt
     7021 tctttagcgt accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca
     7081 cgacgtgtga tttctttttc ttccagttcg atttgttttt ggcgttctac gattttaatc
     7141 tgcatttcct gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg
     7201 tctgcttttg ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt
     7261 tcttttaatg attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt
     7321 tctgcttgag cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca
     7381 tctcttttga cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct
     7441 ctgacctctt tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa
     7501 gcgacttctt gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc
     7561 atagaaccaa gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt
     7621 tcttctcttg tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa
     7681 ccgattttga tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact
     7741 tcaggggtgg agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa
     7801 actggcaaga cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca
     7861 atgtgcacat ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt
     7921 gctgtctggt acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt
     7981 gcgatcgcga taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc
     8041 atgctttcat aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc
     8101 gttccccgct cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa
     8161 ccggtaacac ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca
     8221 acagctactt gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc
     8281 atcggcatca atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta
     8341 gcaataaata aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag
     8401 cttgtgaaag ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat
     8461 acatctccaa aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa
     8521 taaatcgtct caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct
     8581 atcataccaa tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta
     8641 taggaaggtt gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt
     8701 cacaataaat tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc
     8761 tccagaacac gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg
     8821 ccatattgat caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct
     8881 ctagcattgc taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg
     8941 acccgacaca tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt
     9001 ttatcgttgt catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg
     9061 gtcttccttt ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct
     9121 ctgaaaaaga aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc
     9181 aagagattat tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca
     9241 aagatggaca aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag
     9301 gcgccgtttt tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa
     9361 tagcagaaca gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag
     9421 ccatctcttc tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc
     9481 aaacagagca gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt
     9541 tttgtttaaa gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa
     9601 tgggatagaa actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc
     9661 agtcgcaact ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac
     9721 gaaacaatat ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt
     9781 catttctggt ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct
     9841 aaatcaaaat tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat
     9901 tgccggtttt ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt
     9961 agaaagcgat tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca
    10021 agtgttaggg aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa
    10081 cggacaggtc cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt
    10141 ctgcgatgac ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt
    10201 ttcgacgaag ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg
    10261 ccacggtgga tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga
    10321 attaccaaaa aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa
    10381 tggttgcaga attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa
    10441 gtttcagaaa tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc
    10501 tgcttgatgt atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc
    10561 aaggaaatga tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga
    10621 atgtgatccg ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc
    10681 agctggcgat cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg
    10741 atcagaaatt gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat
    10801 tcatcgattt gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc
    10861 taaaagaaga tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta
    10921 tatccgtcaa gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca
    10981 aatatttgga aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa
    11041 agctcacaga ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag
    11101 aagtaacaac tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt
    11161 cttatcttaa ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta
    11221 ttatttcacg aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat
    11281 ttgaacctac atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag
    11341 cagggtctgg aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg
    11401 caaaaacatc ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt
    11461 cattatcttg aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag
    11521 ctaaaaatcc ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac
    11581 tgacaatatc gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag
    11641 cagcttctgt ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa
    11701 cagcttccgt caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt
    11761 cacgactaaa catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat
    11821 acgcagtatt tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg
    11881 gcgaatacga tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga
    11941 tatgattgac tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg
    12001 caagattggt ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg
    12061 ctgacacgat attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa
    12121 tattcaattt ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca
    12181 tactttcttc ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa
    12241 cgatccatac aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg
    12301 ctaaaagtgc ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg
    12361 tagccatcat gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg
    12421 ccgtcaaaac tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac
    12481 tgtaatacca tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg
    12541 cgccgattgc tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa
    12601 atagccacgg tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg
    12661 cacctgtaac tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa
    12721 aagtagcgat aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca
    12781 agccactcat ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa
    12841 ttgaggaata catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa
    12901 aggtcgtaga accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc
    12961 ttaagaaaaa aggttttgaa actagaataa gaataatata aataactaat ccccaaatca
    13021 agacattgat tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg
    13081 ccagtgctgg caaagccact gttaagacga ctttcgatag aatttttcca acagattcag
    13141 aaaaaatccc cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga
    13201 ttgcactgac aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct
    13261 ttctcctcct aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg
    13321 tattaacatt tttaattt
//
LOCUS       DAGCYL010000073        13020 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.074, whole genome shotgun sequence.
ACCESSION   DAGCYL010000073 DAGCYL010000000
VERSION     DAGCYL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.074"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(80..152)
                     /locus_tag="MB216_001540"
     tRNA            complement(80..152)
                     /locus_tag="MB216_001540"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:Lys,seq:ctt)
     gene            533..1102
                     /locus_tag="MB216_001541"
     CDS             533..1102
                     /locus_tag="MB216_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBT4526381.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1089..1949
                     /locus_tag="MB216_001542"
     CDS             1089..1949
                     /locus_tag="MB216_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBT4526382.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2139..2843
                     /locus_tag="MB216_001543"
     CDS             2139..2843
                     /locus_tag="MB216_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4526383.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2848..4683
                     /gene="walK"
                     /locus_tag="MB216_001544"
     CDS             2848..4683
                     /gene="walK"
                     /locus_tag="MB216_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBT4526384.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4680..5996
                     /locus_tag="MB216_001545"
     CDS             4680..5996
                     /locus_tag="MB216_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526385.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5997..6866
                     /locus_tag="MB216_001546"
     CDS             5997..6866
                     /locus_tag="MB216_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBT4526386.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6921..7730
                     /locus_tag="MB216_001547"
     CDS             6921..7730
                     /locus_tag="MB216_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBT4526387.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7833..8462)
                     /locus_tag="MB216_001548"
     CDS             complement(7833..8462)
                     /locus_tag="MB216_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBT4526388.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8632..9924
                     /locus_tag="MB216_001549"
     CDS             8632..9924
                     /locus_tag="MB216_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4526389.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10000..10662)
                     /locus_tag="MB216_001550"
     CDS             complement(10000..10662)
                     /locus_tag="MB216_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBT4526390.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10887..11171
                     /gene="groES"
                     /locus_tag="MB216_001551"
     CDS             10887..11171
                     /gene="groES"
                     /locus_tag="MB216_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBT4526391.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11222..12847
                     /gene="groL"
                     /locus_tag="MB216_001552"
     CDS             11222..12847
                     /gene="groL"
                     /locus_tag="MB216_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBT4526392.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4332 a         2250 c         2815 g         3623 t
ORIGIN      
        1 aaataaaaaa agaacagcgt aaatgttttc gaaatgcttt cgcaccaaca tttacctgtt
       61 ccgtgggttt ggcacccaat gggccgtgag gggctcgaac ccgcgacccg ctgattaaga
      121 gtcagctgct ctaccaactg agctaacggc ccgtacaaaa agtacttaat aatagtagca
      181 ctaagaattt taaaatgcaa gactacattt aaagaaaatc agaaaaatct ttacttcctt
      241 ataggaaatc attcatagat tattcacatt tgctcgctat tatataagca taccaacaaa
      301 caaagttctt gcaagagaac aaaccctaac acttttttca ttttttactc ctttcccgcc
      361 gtctccccgg cgggtctcct gttcttaacg ataaaagaca agaaggctta gccttcttgt
      421 tttttgtttt catggaatcc cgctaatcaa atcacctagt taaagcaact agattgaatg
      481 tttatcataa gtctgatatc atgtttatga aatgaaaaaa ggagcggtta tgatggaaaa
      541 aatgcgtcag caattaatgg agtgggggaa ggaactagaa acattccgtc tgcctcactg
      601 ggaagaactc cctgacttag aactttatat ggaccaagtc agaacgttag ttgatcgtta
      661 tctttcacct gtgatacaag gagaaaagca tccattgctt acctcttcta tggtaaacaa
      721 ttacgttaaa ctaggtttga taccagcacc agttaaaaaa agatataaca aagaacacgt
      781 agcattttta cttgcaatca ctacgttgaa acaagtattg actattcctg aaatcaaaga
      841 agggatattg ttccaaggaa aaacagtagg aattcgtgaa gcctataatc tattttgcga
      901 tgaacaagaa gcagctgtgt ggatggtcag ccaattagca caaggtaaat ctcatccgca
      961 aaaattcgac caggcaaccc ctgtagaata tattgctgta agagcggcaa ctttatcgtt
     1021 tgcaatgaaa cttcttgcgg aaaaaacgat tgttctagaa accgaatatt taaaagagga
     1081 gaaaacaaat gaaaaacaat aaaatcgcaa ttttagtaga ttcaggtacg gatgtgccag
     1141 aagaattgat aaaaaaatac gagatgtata tgatcccatt gaaaattatt tataaagatc
     1201 aggtatatac agataaaatt gacattacct ctgaagagat ctatcaacgt ttgccagagg
     1261 aaatccctgg tacatctttg ccggatggtg agtcgatcac agaaatcttt gagcaaatca
     1321 aaaaagacgg ttatgaaaag gtacttgcgg taacaatttc cagtggattg agcgggacat
     1381 ataatgtggt tcgtttaatt gcacaacagc aagaagaatt ggaagcatat gtactagata
     1441 cgaaaaatat tggtattggt gcaggttttt ctgcgatcca agcagcaaaa tggctggaag
     1501 aaggagtaga atgggatcaa ctgatcagta atctgaatga gttagtcgaa agaacaaaag
     1561 ttttctttaa tgttgcgaca ttggaatatc ttcaaaaagg tggacggatc ggtttagttg
     1621 cttctattgt aggaaccgct ttaaagctta acccaatcat ctcatgtaat gaagaaggga
     1681 tttattacac agtaggaaaa gctaggggac gtaaaaaaag tttggataaa acggttgctt
     1741 acgtcaaaga aaaaatcggt aatgcaaagg tcttcaactt agctgttgcc catggggatg
     1801 cgaaagaaga agcaattgaa atggaagcga gacttaaaag agagttccca caagcaaacc
     1861 aaatctactt tggccagatt tctccggcat tagtagtcca cactggtccg ggattattgg
     1921 gtgtcggtgt acaagtttta gaaggataaa tcaaaaaaag gaaacactga ggtgaaaatc
     1981 agtgttttcc ttttttattg aagttagaac aaaaaataat aaagtatttt gcactttcat
     2041 tttaattttc tcttttgttt tataggcaaa gatgaaacaa aagcagaaat acggtataat
     2101 gagaaggtat gacaataata tataagaggt ggctctttat gaaaaaagtt ttagttgtcg
     2161 atgatgagaa gccgatttcg gatatcgtca aattcaattt agccaaagaa ggctatgatg
     2221 tttatactgc atatgatggg gaagaagcct tggaaaaagt tgctgaggta gaacctgatt
     2281 tgattttact tgatttgatg ctacctaaaa tggacggctt agaagttgct cgtgaggtac
     2341 gcaaaactta tgatatgccg atcattatgg taacagccaa agattctgaa attgataaag
     2401 tcctaggctt agagttaggc gccgatgatt acgtgaccaa gccattttcc aaccgagaat
     2461 tagttgcacg tgtcaaagca aatcttcgtc gcggtgccac ggctgcgaag gaaccagaag
     2521 aggcagcacc tgcggaattg acgattggtg atttgacgat ccatccagaa gcttacatgg
     2581 tgacgaaacg aggagaaaca atcgagttaa cacatcgtga attcgaacta ctcttttact
     2641 tggcgaagca ccttggacaa gtgatgacaa gagaacattt attacaaacg gtttggggtt
     2701 atgattattt tggagatgta cgaacagtgg atgtaactgt acgtcgtttg cgtgaaaaga
     2761 tcgaagataa tccaagccat ccaaattatt tagtgacacg tcgtggtgtc ggttactatt
     2821 tgcgtaaccc agaacaggag taattggatg aaaagaaaag tccgcttttt tcgatcagtc
     2881 aattttaaga tcgctattac ctttattctg attttattga tctcgatcga aattattggt
     2941 gcctacttta tacgtggttt agaacgttcc acgatcaata catttattaa agatatgaat
     3001 caaacggtgg aatcattagc aacaacaatc agcccggaat tgaatcgaaa agataatgca
     3061 gatgatgaag aggtaaatgc gaatatcaaa agatttattg aaaacagtgc gactagcgat
     3121 atcatcgaaa tacgggtcgt tgacgaaaaa gggatcatcc gaggaacaac cgatgtcaat
     3181 gaacaaagcg ctgtgggaaa gaaaaatgat tatgttgata tcaatgattt cacgactaaa
     3241 cgttatgttg ctttagataa cgacgataaa cgggtgaata tcaatgttca accgatcctg
     3301 tccccgaccg gcgatgccgt tatcggggct ctttacgtca aaagtaatat cgaacaaaaa
     3361 tattcggaaa tcaataacac agcagtcatt ttctttactg cctcactaat tgcggcattt
     3421 atttcaatga tcgtatctgt tttagttgct cgttcgatta cacagccgat cggtgaaatg
     3481 agagaacaag ccattcgaat cgctagagga gactacagtc ggaaagtcaa agtgcatgga
     3541 caagatgaac ttggacagct ggctgatacc ttcaatcagt tagctgaacg tattgaagaa
     3601 gctcaagata caatggaagc tgaacgcaat cgtttggata gcgtgctatc ccatatgaca
     3661 gacggcgtca ttgcgacaga tcgcaggggt aaagtcatta cgatcaatga tatggctgtt
     3721 tccttgctag atatcaaaaa cgaggaagca atcggacaat cgattttgac acttttagat
     3781 atcgaagaag aatatacgtt gcggaaatta ttagaagatc caaacgaaat ggtacttgac
     3841 cgttcaacca gtacattgga aagtgatcag atgatcttgc gagtagactt ttctatgatc
     3901 cgtcgcgaat caggttttat cagcggatta gtagctgtct tgcatgatgt gacagaacaa
     3961 gaaaaaacag aacgggaacg tcgtgaattc gtctctaatg tttcccatga acttcgtaca
     4021 ccgttgacaa gtatgcgtag ttatatcgaa gcattgagcg aaggtgcgtg gaaagacgag
     4081 gaagtagcac caaatttttt gaaggtaacg ctagatgaaa cagaccgaat gattcgaatg
     4141 atcaatgacc tcttaaatct atctcgcatg gatacaggta atacccagct tcaattagaa
     4201 tatgtcaatt tcaacgagtt agtgaatttt gttcttgacc ggtttgatat gatggtggga
     4261 aatcaggaaa aaaattataa gatccgtcgt gaatttacgc aaagagatct ttgggtcgaa
     4321 gtagatacag ataaaatcat ccaagtagtc gataatatca tgaataacgc gatcaagtat
     4381 tcaccagatg ggggaacgat tacttgtcgc ttattagaaa cgcataataa cgtggtttta
     4441 agtatcaccg atcaaggatt ggggattcct aaaaaagatc tgaaccgagt gttcgagcgg
     4501 ttctatcgag tagataaagc acgtgcaaga gcacaaggtg ggacaggact tggattagct
     4561 atttctagag aagtgattaa ggcgcatcga ggcgctattt gggcagagag caaggaagga
     4621 aaagggtcga ctttttatat ctcgctgcca tatgaacctt atgaggagga atggtgggaa
     4681 tgagaatctc tgaaaaagtc gttcgtatag gactcatttt gatggttgcg ttaagcatat
     4741 acttctctta tgcaatctgg ctgagcccag caggaaaaag ttcaatcaac gtagatgaga
     4801 caaacactca aatgatcgag tctcaaagca atcgaaaaac ttccgaagtc tttcttccgc
     4861 ttcatgttac atggcaccat tcgggaatga tccaagaaac caacagtgaa aatctcgtgt
     4921 accgattgca attgatcatc gataaagcaa gatttggaaa gctttctgaa gtagtttcag
     4981 gagacaaatc acagtttgaa caggtgaaaa atctgaactc tggttttgag ttggcctata
     5041 atgcgccttt tttgctaaga gaatacaagg aagcatttga tttagattta gatgtgtcga
     5101 acttggcaaa agaaagcgaa aatatgtatt ttactagaat acaattcgat caaggaaata
     5161 aaaaaatccg atttttgaat tattcgaacc atctcgtata tgaagcaaat atttcgttga
     5221 attggacaga tatcaataaa gaattaacag caaaagacac aaaatggaca gaaatgacaa
     5281 cagaaccatc gacgatcgac acgcaatatg ctacgaaaca ttcgatcaaa ctgaaaaagt
     5341 atagttatat cctgtcacag caaccctata cactttttag aaatgctttt ttccagaaac
     5401 cagatagcgt gaaaaataat gatgaaagca atgaattgat tttttacggt gggaatgaaa
     5461 caatgtctat ccattcggaa acgcaagtag ttgattttcg tggagagctg ccagagaaaa
     5521 aagaaaatgc tagtgtattt tctgaaagtt tcccctatat tagcaaatta ggcagttctt
     5581 tagggaacct cagatatttt gaccgcagtg acaatacgat cgattatcgg atattcgtgg
     5641 aaggcttccc ggtttttggg acagacagca gaggacagat tggtgtggaa gttggcggtg
     5701 aaacgaatgg ttctgtgcaa gtgaatatcc aaaccagttt gaatacgatt caagtgccga
     5761 taccttcaga cgaagaagta gaacttgcaa gtacgaatga aatcatccag cagttaaaag
     5821 ctaagggagc agatagcaac aagattggtt cagtgatcgt tggctataca tggcaaaatg
     5881 tcaaagaaac gaatcgtgtc gttgatctct tacctgaatg gtatgtgaaa tataaagata
     5941 actggtattc agctaacgac cttctggaaa aattacctgg attggaggcg aattaattgg
     6001 atttcaaaaa aatcgaatgg atatttttcg tcgctttttt agggttgaat gtgtttttat
     6061 tcagcagtta tcatgacgcg gtataccaag aaaacaatgt cattcgctct aatcagactg
     6121 aatcaatcca acaacgtctg gctagcgacg atatcactta cacggggaag atttcgtctg
     6181 agaacaagca aggctattat cttagcgcgg agcagaccaa tcttagcact tcattagcga
     6241 attatcgcaa ggctggtaaa agcggcttgc tggcaagtgg tgtgttttta gacggtgatg
     6301 ttttgacgaa atctttatcc agttcagatg ctgaaaagaa cgtgatcgat ccagataaaa
     6361 ttagtagttc cttggaagat tttttatcta acaaagacaa tattttattc ggtaatgatt
     6421 atgtgtacac gaaaaacttt tctcatttag aagaagaacc actagaaatc caagcaagcc
     6481 aagagtatga aggcatccca ttcaatgatg atacagcaaa aatcgttctt tctttagaaa
     6541 aaaacgataa tgattatagc atcacgaaat acatgcagac gcatttatct gatatcgagc
     6601 agctgcgcga aaagacggaa ctgtatacgg aagaggaagc aatcaataca ttgtacatca
     6661 ataacaaaat accaagagga tcaaaaattt tatggcgtca gctagcttat tcctgtattc
     6721 tgaaagtccg ggaaaaaaat gtgtatgttc ctgtttggca tgtagcgatc gaaacaagcg
     6781 ataaagtggt ccaaattgag agtgtcaacg ctttcagcaa tacgatcgtg acaaacaata
     6841 ctgtaccaaa agtggaagat caataaaaaa gcgagtataa tgaaaaaagt ttacaaagta
     6901 agaagggaag taggagtcaa atggctgaag aaaaaccagc ttttaaaatc agtatcctag
     6961 caagtggaag ttcgggtaat tcactttata tcgaaagtga aaaaaaacgg cttttggtcg
     7021 atgcaggact tagcggtaaa aaaatcactt ccttgatggc tcaaattgat cggacaccgg
     7081 accaattaga tggaatactt gtgacgcatg aacatcgaga tcatatacat ggcgtgggtg
     7141 tcttggctcg taagtatcat ttggatgttt ttgccaatca gcagacttgg gaagcgatgg
     7201 aaccactgat tggaaatgta ccagtggaac aaaaacatct atttgagatg gggaaagtca
     7261 gaacattcgg cgatatcgat attgagagtt tcggcgtctc ccatgacgca gcggctccgc
     7321 aattttatcg tttccataaa gaaaatcggt cattcgtcat gttgacggac accggatatt
     7381 gcagtgatta tatgcgaggt gtcattgaaa atgccgatgg ttatttgatg gaaagtaatc
     7441 atgatcttga gatgttacgc atgggacctt atccgtggaa tttaaaacag cgtattttag
     7501 gagatagagg acatctttcc aacgaagacg gtgcacttgt catgactgat gtgatcggta
     7561 atcggaccaa acgaatctat cttggccatc ttagcaagga aaacaatatg aaagaacttg
     7621 ctcatttaac aatggaaaat gttttgaaag aaaaagattt tggcgttgga catgattttg
     7681 aaatctatga tacagatcca gatgaagcaa ctgagctatt ttcgatttaa acagttcaat
     7741 caataaaaat ataaaaagca gaagataaaa tcaatcaggg ttggttttat cttctatttt
     7801 tattttttta gcatattaaa aaaataaccg tattatcgac tgactacact gatgtgctgc
     7861 ttttcatgtt tctcatccag tatctcatct actaatgcaa tcgcgtaatc tgccatgctg
     7921 atcgtactgt cgccagcagc gtttctttgt aaataatcat cacttaacaa ataagtacct
     7981 gtacgtttgc catgtggatc aaataaagaa gctggactga tataagtcca atgtagattt
     8041 ttagattgtt tcaattttaa caatccttcg tacatattga aagctgttgg atagaagggt
     8101 gcatttttag gtacttggtc aatcatacga gtcgtctttt ctgtatctaa gaataacgaa
     8161 gaggcacccc caacgacaat taagcgtgta tcggttcctt caagcatctc aatcaaatgg
     8221 gacaaagaag tttgatgcat ttcttctttt cctttgggag cgttgaaagc atcaacgaca
     8281 acatcaaaag atgcaagatc agtagttgtc aactcaaata aatccttttg tataaacgga
     8341 acatctgatg gcaatttttc tggatgacgc acgattgccg tcacgtctaa atctctggac
     8401 aatgcttcgt ctaaaataaa tgaacctgca tggccagtag ctccaatgag tgcaattttc
     8461 ataaaaaacc tcctttttta ttcctgtcca ttgtaagcag ttgctgaata ttcgtcaaag
     8521 aatccgattt ttgaaaagct cagtgcgcat tcctttcatg gttcgtgatc attgggtata
     8581 gttaaattaa gaaaactgat tgataaaaat agaagttgaa ggagataaga aatgaaagag
     8641 tggttagatc gtgccttttt accaatgata acatctgttg tgaatcttag acccttaact
     8701 gctttgcgta atggaatgac ttacacgatc ccaataatta ttgttggctc gctgtttttc
     8761 ttatttgctc attttcctgt ggattacatc gctcagtgga tagacaaaac aggattttct
     8821 atgtatttta cacaagtata ccaggctaca tttgatgtga tagccttttg gatcgttttt
     8881 gccattgcat acgcttatgc aaaagaagaa gacatcgatc gcttaccagt cggcataaca
     8941 gccttgtctg cttatctact gcttattcag ccactaaata ctcagataga tactgtctgg
     9001 acaggtgctg aagggatact aggagcagtt acaactgggc tcatcatcgg atggggctat
     9061 gcacgtttag taagacttgc gaaagggtgg aagcaaagag agaagatacc tgaaaatgtt
     9121 tggcagtctt ttacttcatt gattccaatc attgttatag tcacttttac gcttatcggt
     9181 tcagcggttt ttcaacagtt gttcagaatg actgttgttc aaggagtatg gacatatgtc
     9241 cagcttccca ttcaatcgtt catagacacg ttgtttggtg tgcttttatt aggttttttt
     9301 gttccgtttt tatggttatt tggtcttcat ggatctgcga tggtcaatgg gttggttagt
     9361 cccgtcttgc aagcgaattc attagcgaat gcagaaatct tagcctcagg acaagagttg
     9421 acggtggcaa atggcggaca tatcgttact cagcaatttc tcgatcagtt catgacggtt
     9481 acgggtgctg gattgacgtt aggggctgta ttttttatga cggtttttgc gaaatcgaag
     9541 aaatacagag aattaggaaa tcttgctctt ttaccagcct ttttcaacat caatgaatca
     9601 attatttttt ccacaccgat cgtgatgaac ccgatgatgg ctgttccttt tattttcgca
     9661 ccggtcgttt caggattgat tacctattcc gctttatatt ttggattcgt tcctttattt
     9721 acagcagtcc aagtcccttg gacaacaccg ccgatcctct cgggtttctt agtaggcgga
     9781 gtgcctgcag cgattttaca aggtatcgtg ttgtcaatta gttttttcat ttatttccca
     9841 tttttgaaaa aaatagattc tgcaaactgc aaaaaggaaa gacagacaaa aaatgatgga
     9901 gcagcagaaa agatgaacga ctaaaaaaca aaaatactcc tgtcgcttta gattgggaaa
     9961 atcaccaatc taggatgaca ggagtattta tagtcatgtt tatactagcc ctttttggac
    10021 agctaattga acaagaacaa ctaatgtgtt cattcctacg tgtgtgatca tcgatgtcca
    10081 gatccgacca gttgctttgt actctaggct gaagaagaaa cctaggaaaa agtagattag
    10141 cagatgacca tcattatgag caaacgcaaa cagcaaagag ctgatcactg ctccgaccca
    10201 aaaatttgaa tatcgactga tgatccccag tatactgcgg cggaatacga actcttccat
    10261 gatgggtcca gcaacagttg tcgccaaaat aaaagcgggt gcttccatga tcatctggat
    10321 gatattttgt gtattctcgg aaggggttgc tttgccaaac aatacagatt ctaacatgac
    10381 agcgatgctt tgcagaatca atgcgacaaa gattccaacc aatccataaa taacaatctt
    10441 tggagcagaa gcttttttta ccgctgattc gatcgctaag ggttcacgtt gcttaaaata
    10501 aaaaatagct aggacaacgg cgcctagtat atacatgact gcagttgctg tgatcaattc
    10561 agacgtagat tttacaagtc ctatcgaagc taggatcaag ggtgagataa aaattagacc
    10621 atagcaaaaa atgctgagga agctatattt ttttaaagac atgtaattcc tccttttaag
    10681 ggataacaag catgcaaggc aagcttatat cgattgattt caattatatc ataatttcaa
    10741 taagcagctg tcagaataaa atttcttttc tttgatttaa aagcttgcaa aaaagaggga
    10801 gaatgagtat tattaaaaat gtaggttagc actcgaaggt taagagtgct aacagattaa
    10861 aaaaagagtt atcggaggga tttatcgtgt taaaaccatt aggtgatcgc gtcattatcg
    10921 aagtcgcaaa agaagaagaa aaaactgtcg gaggaatcgt tttagcttct gctgctaaag
    10981 agaaacccca aactgggaca gttgttgcag tcggtgaagg ccgtttgtta gaaaacggtg
    11041 aaaaagttcc tgccgctgta aaagcaggcg accaagttat gttcgaaaaa tacgccggta
    11101 cagaagtaaa atacgaagga aaagaatatc tgatcgtcgc aggaaaagat atcatggcga
    11161 ttgttgagta aatttggaag ataaaaaaat aatcaaaaat aatcaatgag gtgaatcaat
    11221 catggcaaaa gaattgaaat ttgcagaaga cgcacgcgca gctatgttac gcggggtaga
    11281 taaattagca gatacagtga aagtaacatt aggacctaaa ggccgaaatg tcgtactaga
    11341 aaaatcttac ggctctccat tgatcacaaa tgatggtgtg acgatcgcaa aagaaatcga
    11401 attagaagat cattttgaaa acatgggtgc aaaacttgta tcagaagtag catcaaaaac
    11461 aaatgatatt gcaggagacg gtacaacgac agcaactgtt ctgacacaag caatcgttcg
    11521 cgaaggttta aaaaacgtaa ctgcaggtgc taatcctcta ggcattcgtc gcgggatcga
    11581 attggcaaca aaagcagcag tcgaagaatt gcataatatc tcaactgttg ttgattcaaa
    11641 agaagccatt gcacaagtag cagctgtttc ttcgggttct gataaagttg gacatttgat
    11701 tgctgatgca atggaaaaag ttggaaacga cggtgtcatc acaatcgaag aatcaaaagg
    11761 gatcgaaaca gaactagatg ttgtagaagg tatgcagttt gatcgtggtt acttatcaca
    11821 atatatggta acagataacg acaaaatgga agctgttcta gaaaacccat atatcttgat
    11881 cacagacaaa aaaatctcta acatccaaga tattctgcca ttgttagaac aaatcttgca
    11941 acaatcacgt ccattgttga ttattgctga cgatgtagat ggtgaagcat tgccaactct
    12001 tgtattgaac aaaatccgtg gaacattcaa cgtagtagct gtaaaagctc ctggtttcgg
    12061 cgatcgtcgt aaagcaatgc ttgaagatat cgctattttg acaggtggta cagtaattac
    12121 tgacgatcta ggtttagaat tgaaagatgt aacaatcgaa aacttaggaa atgcttctaa
    12181 agttgttgta gataaagaca atacaacgat cgtagaaggt tctggagaaa aagaagcaat
    12241 cgaagctcgt gtgcaattga tcaaaaacca aattgctgaa acaacttctg attttgaccg
    12301 cgaaaaatta caagaacgct tagctaaatt agcaggtggt gtagctgttg ttaaagttgg
    12361 tgcagctact gaaacagaat tgaaagaatt aaaattacgc attgaagatg cattgaatgc
    12421 tacacgtgcc gcagtagaag aaggaatggt ctcaggtggt ggtacagctc tagtgaacgt
    12481 tatcagcaaa gtttctgctg tagaagcaga aggagacgtg gcaacaggta tcaagatcgt
    12541 tgttcgtgct ttagaagaac caattcgtca aatcgctgaa aacgctggtt atgaaggatc
    12601 agtgattgtt gataaattga agaacgtgga actaggcact ggtttcaatg ctgctactgg
    12661 agaatgggta aacatggttg aagcaggtat cgtagaccca actaaagtaa cacgttctgc
    12721 cttgcaaaat gccgcttccg tttctgcctt gttattaacg acagaagcag tcgttgcaga
    12781 taaaccagaa ccagcagcgc cagcagctcc tgcaatggat ccatcaatga tgggcggtat
    12841 gatgtaagaa aaagcaactt gcttatctac aactgatcat tagtgaaaga cagataaaaa
    12901 gaggctggaa cagaagcgtt taactccaag aaataagaag aaattcacgg aaattgcttt
    12961 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgctgtttat
//
LOCUS       DAGCYL010000074        12908 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.075, whole genome shotgun sequence.
ACCESSION   DAGCYL010000074 DAGCYL010000000
VERSION     DAGCYL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.075"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            127..3654
                     /locus_tag="MB216_001553"
     CDS             127..3654
                     /locus_tag="MB216_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBT4526393.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="MB216_001554"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="MB216_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBT4526394.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="MB216_001555"
     CDS             7442..7783
                     /locus_tag="MB216_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4526395.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="MB216_001556"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="MB216_001556"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBT4526396.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="MB216_001557"
     CDS             9140..11560
                     /locus_tag="MB216_001557"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBT4526397.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="MB216_001558"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="MB216_001558"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBT4526398.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4386 a         2243 c         2738 g         3541 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaacttta
//
LOCUS       DAGCYL010000075        12298 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.076, whole genome shotgun sequence.
ACCESSION   DAGCYL010000075 DAGCYL010000000
VERSION     DAGCYL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.076"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(320..934)
                     /locus_tag="MB216_001559"
     CDS             complement(320..934)
                     /locus_tag="MB216_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4526399.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1540..1917)
                     /locus_tag="MB216_001560"
     CDS             complement(1540..1917)
                     /locus_tag="MB216_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526400.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2217..3098)
                     /gene="galU"
                     /locus_tag="MB216_001561"
     CDS             complement(2217..3098)
                     /gene="galU"
                     /locus_tag="MB216_001561"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBT4526401.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3141..4160)
                     /locus_tag="MB216_001562"
     CDS             complement(3141..4160)
                     /locus_tag="MB216_001562"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBT4526402.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4793..5797)
                     /locus_tag="MB216_001563"
     CDS             complement(4793..5797)
                     /locus_tag="MB216_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4526403.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5810..6859)
                     /locus_tag="MB216_001564"
     CDS             complement(5810..6859)
                     /locus_tag="MB216_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4526404.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6876..7697)
                     /locus_tag="MB216_001565"
     CDS             complement(6876..7697)
                     /locus_tag="MB216_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBT4526405.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7684..8463)
                     /locus_tag="MB216_001566"
     CDS             complement(7684..8463)
                     /locus_tag="MB216_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4526406.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8480..8950)
                     /locus_tag="MB216_001567"
     CDS             complement(8480..8950)
                     /locus_tag="MB216_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4526407.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(8962..9381)
                     /locus_tag="MB216_001568"
     CDS             complement(8962..9381)
                     /locus_tag="MB216_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBT4526408.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9462..12239)
                     /locus_tag="MB216_001569"
     CDS             complement(9462..12239)
                     /locus_tag="MB216_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBT4526409.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
BASE COUNT         3585 a         2596 c         2020 g         4097 t
ORIGIN      
        1 ggaagactct ttcatcaacg tgtattaaga gacaagaaag aatccatatg gattctttct
       61 tgtctctttt tccactactt tttatttttc tgacactaca tattcttata actcatttct
      121 cataactgtg aaacaactta taaaaataac aaactttaaa caaaacaagc ttaaaatcaa
      181 agatgatcca tcttaaaata actaaaacaa aaaaagtagt cgttgttaat cctcattaat
      241 aatcgttttc tttttttcaa acgctccatt tttatgtgga acattatttt tttaaggaac
      301 tttgtcaaca tgtggataac tattttccac ttgtgtcctg gactagctgt tttgctgtaa
      361 aatacaaaac ttgctgcagc aaaaagatcg ttgcaagaga ggctgttccg cagatcgctg
      421 gatctagatt tggcaaagtc aagactgcat catgtaaatt ggcgtggttg tccaaaacca
      481 aatcagcaaa ttcaaataat cgcaaaccac tgctatgttg cgatttgata cttcgtgaca
      541 aatcaaatcc agtaacaatg attaatggat gaccattttc tttgatccac tgagctagtt
      601 cgatgattgc cgggtttctt ccttcattcg aaagaagaag aaaaatgtct tctgatttga
      661 cagaaagctg atcaataatt gcctgtcctg caccttctag cgtttcatag acacctccgg
      721 ctggattttc aatcaatata cttggtacaa gatccggggt ttgatcccaa aattcaaatg
      781 ctacaccact tagtgtgcgg ctagcatata tctgcaatct accaccttga ttaatttgtt
      841 tcgcgatcca agtgccagct tgaagcatca gcggctcttc tatccttaat ttctcttcca
      901 attggtgata atagtcaaaa taacataaac tcatttatct ctcccgccta gctatacttg
      961 acatgaatag aaacagctgc ctaatctaag gaagtacata aaaaaacagt agttagacag
     1021 taacacatgt ggtctttgct gtttcaaaca caaaaaaggc attgaacgaa tacgcccaat
     1081 accttgacat agcaaataaa acaacgtgca gaccatggaa aatatctaca taggggtaga
     1141 attttcaccc tagattaggt acaataaatt aggtcgatgc actgcattga ctatggcaaa
     1201 atgatcggtc agggtgtatg tgtgtgactg gtcattccct gattcttagc gccaactaag
     1261 ggtcagggag ccttttttta ttcagctgtt tctatctgaa tttattgtaa gtaatctctt
     1321 atccaagtgc aagcaagaaa atacaaaaaa acattggtat gatttatata tatataaata
     1381 acataaaaaa aggaatggct tatcccgata caacaagcat tcctttaaaa aagattcttt
     1441 tatataagtg aaaaaagctt ttttccacca attaaataaa tctctcatct caatagttta
     1501 tccattattt tatctttttt tgcgtatacc tactaaatat taccgtcttc ttctcgttct
     1561 tctactttgt tcgagagaat ctgtactaga aaaagtagtt gtatgtctac cttttctgcc
     1621 tatccgagac tttcttttca tatatgggat ataaatgatt agatacagta cagcaaaaaa
     1681 taaagcaaat acgcaagctt cccaactacc taacgccaat tcaaccatca aggtagcaac
     1741 cccaacggat gctgcatacc agataaaaat cagaaacgtt tctttttttc tgaccatgcg
     1801 aaacatgata aatagactaa taagcaatag tggcaacaca aatatccaat tgcttccttc
     1861 catcagccac ttgagtatat caagcagcga ctgtgcaaca gcgactaatt ttcccatagt
     1921 gaacacacac ctttttttat aattaaaaaa ttcgcctttt tattctacta tgaatcttct
     1981 ggcttggctt tcatttctct tatgtaatca ttttctttta tacaacttaa aaaaaaatta
     2041 aaccattagc ataatatttt ctgaatctat tcacgaaagt ctttttctat ctttcgtttt
     2101 ccacgactca actgatctag agtcaaaaaa ctaaaacgac agttgcgcaa atgacgcaca
     2161 agctgccgct ttaataacaa atagcctttc attctcagca ggccacttat ttccagttaa
     2221 ctttctgttc ttagcttttt actcaactgg ataatatatg cacttaatga agtcccaatt
     2281 tccgaatgtt gaagtccata ttcaatattt gtcttcatat atcctaactt atcgcctacg
     2341 tcgtatcttt ttcctttaaa ctctctcgca tagaccgcct ctgtctggtt aagagtctga
     2401 agtgcatccg ttagctgtat ctcgtttcct gctcctggtt cttgagtttc taatatctca
     2461 aatattttag gagtcagtag ataacgtccg ataatggcta aattacttgg tgcttttccc
     2521 atttcaggtt tttctacaaa cccttttact ctgcaacgac ccttttcgct atctgtttta
     2581 ggatcaataa ttccgtattt agatgtttca ttttctggta cacgcatgac ggcaacagcg
     2641 gaagcttgtg tttcttcata atccatcatc aattgttgag tcaatgggat atcatctttc
     2701 atcaaatcat cccccagcat gacgacaaac ggttcatttc ctataaatgc ttttgcttga
     2761 agcacggcat cccccagtcc tcttggatga gattgacgga tgaagtgaag attgacttga
     2821 gtcgtttctt caactaaagc gagtaattct gttttttcct tttcccgcaa attgttttcc
     2881 aattcgatat ttgcatcaaa gtgatcttca atgggtcttt tttcttttcc cgttacgatc
     2941 aagatgtctt cgattcccga atccaatgct tcttccacaa taaactggat cgttggttta
     3001 tccacgattg gaagcatttc ttttgccatg gcttttgttg ccggcaaaaa acgtgttcct
     3061 agcccagcgg caggaatgac tgcttttttg accttcactt gctgttcctc ctaaacgacg
     3121 attaactatt gatcatctat ttaacgaaga gaaaattcat tctccatttt tccgtcacgt
     3181 aacatgattt cttttgctgc ttttttgata tcttctccat tatacaaaac attatagatt
     3241 gtttgagtaa ttggcatctc aacacctagc tgttgagcta gctctactgc tgcttttgtc
     3301 gttgccacac cctcaacgac cattcccatg ttttctagaa cttcttccag cttatgtccc
     3361 tgtcctaaca agttaccggc acgccaattg cgagaatgaa cacttgtaca agtaacgatc
     3421 aagtccccca caccactcaa accgataaat gtcagaggat tggcacccat cgcgacacca
     3481 agacggctga tttctgccaa tcctcgagtc atgattgctg cttttgcatc atctccgaat
     3541 cctaatccat ggatagcgcc ggcaccaatt gcaatgatat ttttcagtgc tgcaccagtt
     3601 tctacgccaa tcacatcatt atttgtataa atcctgaaat aatcattcat gaacagtttt
     3661 tgtacatagg ctgctgcttt ttgatcaata cttgcagctg taattgttgt gatatcatgt
     3721 actgccactt cctcagcatg gctaggacca gataatacaa caattgcctg tcttttttct
     3781 tctggaattt cttcagccaa aacttctgag attcgtttat gcgtatcttg ttctagtcct
     3841 ttacttgcat gtataatgat tggcttagta ttcattttac caataagttc ctgtgcgact
     3901 gaacgaatcg cttttgttgg aacaacaaat aaaatagcat ctgcgttatc cactgcttct
     3961 gccaattctt tatatccgat aatcgattct gggatcgtca aatcaggtaa aaaatgctga
     4021 tttgtatgat acttattgat ttcatcaatc tgttcaggta catttcccca aatacggaca
     4081 tcatgcccgt tttctgccaa tgtttgtgct aaagcagtgc cccaagatcc aggaccaaga
     4141 actgcgattt tttgtttcat ggatattcct ccaaataaat aattgataat ttccattata
     4201 gtatacattt cttaaagttg ggactaattt ccattgtttt taagtgtttg tttacttctt
     4261 ttatcgtttt tacttgaaat aatcaatgat atgttacaat agtctacatt ttatttttac
     4321 actcgttatg ctttgcttgt tcctaagagg aagcagtttt gacaatgtta ctaccagcat
     4381 tactattagg aggttaggaa acttctccta gtgagtattg atttaaaata cataagagac
     4441 agattgaaca tttctaaatc tgtcaggtcc tgcaacggac agattgtctc tgttgacggt
     4501 tgacatagga acttttgttt tagtcaacat tctggataca aaacgctatg taaactggcg
     4561 caactcgttg tacttggact tttactacgg atccatgcaa caactaaata gtactattgc
     4621 ataacaaaaa atggttggca cccacatgtc ccattaaaca gattataagt tgaactgtag
     4681 ataatgcaaa aaatgtgttt tataacgtaa ttgatgccaa tattttaaaa aaataaaggg
     4741 agcagttatc gctgctctct ttatttttta tattcttttt atccagtcta tcctaataat
     4801 caaactgacg atagtagcgg cgtatctcta atggatggca gttcaactgc tccacatgag
     4861 catcgatgcg ttgtgtcact acatgcatca ataagaaaga caaaacacgt ccgcgatatt
     4921 tttcactgat accttctact ggatagtctt ttgtgtctat caatgtataa ttgctgcaaa
     4981 tttttggcaa aagtttagcc acacgttcag ataacactct ctgttcatct tcgcctaaaa
     5041 ataatgtgac tggggttgtt tcttctacga tctctaacgt cccgtgaaga aactcggcag
     5101 catggataga tttcgatgga agccagcttt gttcttccca gtaacacatg gcatatgaat
     5161 aaacagctcc ccattgattc cctgctccaa taaaataatg catactatca cgtcgatgtt
     5221 tttctgcaaa tgataagcca aatgcatctg cttttttctc tgcttccact aaccctgttg
     5281 gtaaataacg ttcgagctgt tcatagtatt ctgaataagc ttcgaattct ccattcaaat
     5341 acatcaatcg atctgctgcc ataaaaaatt tgatttgttc tgttccagga gctggataag
     5401 tgaccacaaa atcgcatagc tgagacaact tactgttcgc tttatcgata aatccaatca
     5461 gtgtcgctcc tactttcttg atttgttcta ctgctttcac tacttcttga gttgtaccgg
     5521 taacagagga caaaataacg atagaatctt ttgttaatct tttgtttcca gtagtgtaat
     5581 attctgcagc atgctgtaca aagacaggca agttgctttt cccattcata taagtgacag
     5641 cttgcatcga ggaagcatac gttccaccga ttcctagata atagacagcg tcaaattttt
     5701 ccgcataaag tttatcgatg atttcttcta cttgcggacg taaagctagt gcgcctttta
     5761 ttgctttgat ttgttcttct tcattgaatt tcagcatgat ttccacctct tataatttac
     5821 ttttcaagac tgtatcaaag tcttcaaaaa tctttttgtt caagtcaata cctttagcta
     5881 ctgcggtttt ataagcgaat agttgaaacg gaacgacaag cgctaataca gacagatatt
     5941 catcacattc tattgctaat ccaagtgttt tctcgtttct tgcttttttt gtggtaatgg
     6001 tgagagtctc tccaacgtac cgactcatat atctttgcaa cttttgcgaa cgttccttat
     6061 ttacactttc cccttcaata aagaatagca aatgcgaagc atctgcctcc aggtatgggc
     6121 cgtgcatata agcttctaac tcaaatcctt gtgacggaac gcggattgtt tcagtcaatt
     6181 tcgtctctgc ttctttagct gttccccagt ttggaccata tccaatggtg acgaaacgct
     6241 gtgctaaacg aaataaactt tgatatccat caaaaaattc ttccgttttt tgaataatcg
     6301 ctggtaaatg gttgattatt ttgcgcaact gttgcatgta gtcttgttct atttttttgc
     6361 tgattttatt tttacttatt ccaattccta tccctaagag tagtaactgt aaaacagtcg
     6421 cagaatagcc ttttgtaacg aagcctactg attctacccc ggcacctaaa tcaataattt
     6481 gtgcagcttc taaagcaagt ggactttgca cgtcatttgt gatagcaaca acaggtaacc
     6541 cttgtttttg tatcattttt actgcttcaa cagtagaagc acttttcccg ctttgtgaca
     6601 cagcaatcac agtatcgatt gaaggagaaa gttttccata attgaaaaaa taaaaaggtt
     6661 cttgaatatc gacagtaaga tcaccataag attccaatgc tggcttcgct gctaaacaag
     6721 cgttataaga tgaacccgtt gctaagatca gtaaattcac tgtttttttc atatgttctc
     6781 ttgtcgaaaa atcattcttt ttcaagatcg tacttagtac ttccttttct tcatagatat
     6841 aatcttgcat cgtaaacatt ttctctgctc cttttttaaa aaatccctat ccacgaacca
     6901 aaaattccaa agaaagctag tccgatcaag atcgttaatg gtttcacttc tttcttcaat
     6961 agataaaaca ctatgccaaa tgctccaaga cataagatat ttggaacgat atcatcaaaa
     7021 atactttgaa cagtcacagc attttctcct gaaccgattt ttaaaggaat attgatggtg
     7081 atcatcgtgg cagtcattgc tccaacgacc atcagtccaa taatagacgc gcctaatgtc
     7141 aaactttcca tcataccgtt tgcctggatc tttttcaaaa aacccgttcc taagcgatat
     7201 ccccaacctg ttgctagata gcgaaacaga atgtgcggaa tattgaaaat caaaatgaac
     7261 aagatagggc ctaaaatatt tccttgcatg gctaaagagg tacccacacc tgtggcaatc
     7321 aatcgcaaag ttccccaaaa gaaagaatct cctaaccctg caagaggtcc catcaaagat
     7381 gttttgatac tatcgatcgc agtcacatca aattcaggat catccttatt ttcttcctcc
     7441 attgcagccg tgattcctaa aatcagtgta acgatatgtg gggtcgtgtt aaaaaactct
     7501 agatgccgtt tcaaagctgc tgccatttct tctttcgact gatataactt tttcaaaatc
     7561 ggaatcattg cataaacata agccatattc atctgacgtt cataattcca agaaaactcc
     7621 atttgaaaag aacgccaaaa tacgtgattt agctcttttt tcgttatttt gctgtttttg
     7681 atctcagaat tcttcatctt cttctacctc ccctgcagtt gtttgaagtg gtagttgacg
     7741 gacattcata atattgacta ccacaactgc taaaatcgca ccaaaaatag ctactccggt
     7801 cacaggaatc tctaagtaag aagctatagc aaaacctaag aaaaaatatg gtgcaacttg
     7861 tttgttgatc agcaaacgag caagcatcgc aaaaccaaga gctggaatca accctgtagc
     7921 tactgacaac cccttttgaa cgaagtcagg aatcatattt aatagatttc cgatcgtatt
     7981 gcttcccacc atataagaaa ccattaccac aacaccaagc atcaaggaaa gaccgatacc
     8041 ggcaagcaag tgcatatgct cgactccttt ataatttcct tcttctgcat aattgtctgc
     8101 tttatggttc ataattggaa tcaaaagccc taagtaaata tttttcaata tcaatgtcaa
     8161 cgtagcaatc ggcaaaccta gtaaaagcgc ggtttctgta ccggctccag ctgtaatagc
     8221 aaaagccgtc cctaaaatcc caccggtcac aacatcaggt ggaatcgaag cacctactga
     8281 gaacgatccg ataaatgcta actctaaagt tgcgcccata atgatcccag tttttacatc
     8341 acctaacacg attcctgtaa acagccccgt cacaatcgga cgagaaagca acgatgtccc
     8401 tagtgcatat tctgattgtg cgacaaatgc aacaattcct aataagactg cttgtacaat
     8461 cattgaggtt cttcctttct tacaaaactt ctcttacatc cactttttta tctgctggta
     8521 cttgacggat ttctacttct atttgtttgt caattaatcg tttcaagcgt tcctcctctt
     8581 ctggtaaaat atttaccgct tttccgatgc tgcgagtacc aggttttgcc ttgatccctc
     8641 ctaagttgat ggttttgatt tctggatacg cttctgccag cttatatgca tcctcgatcg
     8701 attccactac gatgaataac ttatatttat ctgtcacccc gcttttcaac gcggcaatcg
     8761 agtcatcaat tgatttgatc actagtttga ctccttgtgg cttggctaat ttgatcgttg
     8821 tctttcgtat ctcattcgta ggtacatcat cattagcaat caaaatacag tctgcgccta
     8881 ttgtttgtgt ccatgaaaaa gctacttgtc cgtgtaataa ccgatggtcg actcttgtca
     8941 atacaatcat ttttcttcct cctaaaattc gtcttcttct attttctgat tgagccgctc
     9001 gttacaaaac tggatcattt tttttgaatc agttagcacc tgctggatca aaacagttgt
     9061 ctctacttca gcgtccaacc gagatactag ctcaatcaat agtggcaaat tcaatccggc
     9121 aacgagatag acatgatccc gattgatata ttgtaagaat tcgttgttga cacttccgcc
     9181 aaaaagatcc gtcagaacaa tgatttcttt atctttgtac ctcataaaaa gctgccttac
     9241 tgtttccgct aaatcaaaat tctcatcagt atagcaatcg aatacagtaa tagaatgatt
     9301 tttcccatag atcagctcta atgaatctaa tatccctgaa gcaaattttc catgggaagc
     9361 taagataatt tctctatcca ctttttcatc tcctcttttc acttacacat actaaaaaag
     9421 caagttgcat gccaaaatta gtggcaacaa cttgcttatg ttcaaaattc ttcttctatc
     9481 gagagaaact ctgtatcttc actcaaaata tcatagatat aggcaatctc agaattgggg
     9541 atcatgacac tataatgctc ttcaatgaca ctaaacgcag atttaatagc agataaccct
     9601 tctttttgac actgcttcca tttctctaat ccttgatagg tttcgattgg catttgtcgg
     9661 atcaatcgtt caatgagaca gctcacatgg acatacaacg ctaatttttt gggattgctg
     9721 atgcttcgat tcaactgcac ctctaattct ctcataaaat gatcgatttc tcccatcacc
     9781 ttctctgtat ctagaatagt cacggaatcc agtacttttt ccaatgaaaa attacgaatg
     9841 atgcgctgat tgaatatttc cttctctgaa gcattcatag tagtgtccaa ccattcagct
     9901 agtacagaca tcttttcccc cgagatgagg tcttctaatg aaagatagtc gatattggtg
     9961 atatcaggat ctgtggtgcc aatcattcct acgacttcgt atatggaaaa taatgggtca
    10021 ctgtttttct tatcttttaa ttgctgatat tcatatggga tgattttcaa ctgacaagcc
    10081 gtaggtaagc ttttttctag cagttcactt aagtggaccg ccgtaccgat tccagtgaag
    10141 caagtagtaa tgagtgcttt gggtttgttt tcctctggat agagcagtcg ccattctggc
    10201 tggatctgtc gaacagtctc ttctgctatc tcttctaatg atacattttt ttgcaggcat
    10261 tcccctactg tgattgctaa aggtgtcgtc acattattca aaatcaaaag cggtacggat
    10321 aactgttttt taaaaaactg atggatttct ttcagagatc ccatgtctac taatatgatc
    10381 aatccatttg aaacatcgct gttttcgcaa taatcaagga tttctgaagc aatctgctgt
    10441 ggtgtgacat ctagtggcat atcaaatgat tcaaatagat cctcttgtaa taaacgattt
    10501 actacatttg ccatactgct agcagttgca taaccgtgag cagcaatcat cgcttttggc
    10561 agtcgttttt cttttgacca tttcgctttt tttagataga tcgtcagtaa aatccgatcc
    10621 atacttgtgt cttcaatatc taatttgatc ttacttaatt ccagaattcg ttggacatag
    10681 tgataactag atggataaga ctgagaaact tgttgttcta attctttgat tagttggata
    10741 atatcatgtt cttccggtaa ccatttgata gtgcttcgct gatagagata gtaactcaag
    10801 gcatagacac tgtttccatc gaatttgatt tgataggcag tttccatttg tcgaaaggtc
    10861 tctcggatag attgtgtcca ataaaccagc agctcgtgtt tttcttgtct actggtctca
    10921 aacagcaaaa aatcaaataa ttgaatcact tcttgcttta attttccttc gcaagaatat
    10981 agcagacagt tatttttctt gaaatcaatt aggatttgct caaaactttt aacgattcgc
    11041 tgctgttcgg gctgttgctt ctccattaat tgctctaagg tctggtcttg cgtaatatag
    11101 attccttgct gtgctgtatt tttagtaata ggctgcgcaa gtagttcttt ttgcaaatga
    11161 tggatcgtga catggatttc aggctgttca cgctgttcag catatgcttt tgctgctgaa
    11221 actttgacga tatttttcag ctctcctatg tttccggata atttctgact tgttaaaagt
    11281 gacaaagctg gtccactgac tgacaaaggg ctccccattt ttctttgttc ttcgagaaaa
    11341 aaagagtaaa tcagttctag ccgttcatta cgactacgct gattaagtgc aggtagttcg
    11401 attcggatag gtattcttcg aatgaaagtt gttaagaaat gactagtaat ctcttcagtc
    11461 gtagcaaaaa agagacgtgt tttgatccgg cgtgacaaat taggttctcc catacgataa
    11521 atcacacctt gatcaagata ggtgaacagt ttttcctgac cttctggtgt taagcgatgt
    11581 acctcgtcta aaaacaagat cccaccatct gctgcttcaa aagccccttt tttattttct
    11641 tctgcaccag tgtacgctcc cttaacatgt ccaaacaaat tgctagtcaa aagttcagga
    11701 ttatctgcat attgtgcaca attaaaagtg atgaatggtg cagaatcttc tagcagatca
    11761 tgaagcagac aatactgata gaccaaatta actaaataac ttttacctgt gccactttcg
    11821 cctgtaatca aaagtggtaa tccgccatct ggatagtaaa gagctgtttt gatttgttcg
    11881 atactttctt tcaaactggc gtcgtatccg atcataaaag agaataagtc ttttggttgc
    11941 gcgaaaaaag gctgttcctc tttgagatca tcgatagaaa aataaaaatt cgtgcgtaac
    12001 ttaaaaaatt gtttttcaaa agcttctttg tggaaataaa tcactggtcg agaattgatt
    12061 ttgactagta gttgctcatc atttaactgg ttcaaataat gactgactgt gtttcttttt
    12121 accccaaaat gatcagctaa cctttgtgct gtaaagacct cgttcatatg ttctaaatcg
    12181 aaaaaggctg tctggttctc taagtaacat aaaatttcct tcttcaacaa cttattcacc
    12241 cctcctccat catagaaaac ggattcattt tgtcaataaa atctttaata aagaattg
//
LOCUS       DAGCYL010000076        12198 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.077, whole genome shotgun sequence.
ACCESSION   DAGCYL010000076 DAGCYL010000000
VERSION     DAGCYL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.077"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(160..324)
                     /locus_tag="MB216_001570"
     CDS             complement(160..324)
                     /locus_tag="MB216_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526410.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            619..1947
                     /locus_tag="MB216_001571"
     CDS             619..1947
                     /locus_tag="MB216_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBT4526411.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2149..2982
                     /locus_tag="MB216_001572"
     CDS             2149..2982
                     /locus_tag="MB216_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBT4526412.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2998..3735
                     /locus_tag="MB216_001573"
     CDS             2998..3735
                     /locus_tag="MB216_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBT4526413.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3890..4858)
                     /locus_tag="MB216_001574"
     CDS             complement(3890..4858)
                     /locus_tag="MB216_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBT4526414.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5090..5923
                     /locus_tag="MB216_001575"
     CDS             5090..5923
                     /locus_tag="MB216_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBT4526415.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5957..6796
                     /locus_tag="MB216_001576"
     CDS             5957..6796
                     /locus_tag="MB216_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4526416.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6822..7382
                     /locus_tag="MB216_001577"
     CDS             6822..7382
                     /locus_tag="MB216_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBT4526417.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7581..9302
                     /locus_tag="MB216_001578"
     CDS             7581..9302
                     /locus_tag="MB216_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBT4526418.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9467..10609
                     /locus_tag="MB216_001579"
     CDS             9467..10609
                     /locus_tag="MB216_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBT4526419.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10625..11836
                     /gene="thiI"
                     /locus_tag="MB216_001580"
     CDS             10625..11836
                     /gene="thiI"
                     /locus_tag="MB216_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBT4526420.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4154 a         2048 c         2535 g         3461 t
ORIGIN      
        1 ggttcgagtc ctactgccgg agtaaataaa gcttatgcaa agaaaaagaa atccaatcac
       61 ttgggtttct ttttctttta ttattatgat gaatcaagct ttctgttttt gtcacgtatg
      121 ttgtaatctt tacgaaagag tagagttggc tactcataat caaccatact tcccatcatc
      181 gctaaatacg aaataaatca gcattcctac accaatcaaa gcacaagctg caccaagaaa
      241 agggatgaca ttcccaaagt tatctgtatt cataagtatg ccaccaccca caaacaaaaa
      301 gccgccagca atcaggtttt tcattttatt catctccttt cttacgttat agtatttcct
      361 tctttcaatg agtattttga aagctatctt attgtatgat gttcttctag gcctcttctt
      421 tattatacag gccattttcg ttatctcata gaaatattcc tttttatctg aaaactattg
      481 tataccgtat gacatttttc ttcatgaagt tgtaagtata ttctcattca aatgaaaatt
      541 tcatcataga agagattgta tttctaaatt cttaagagta aaatacgaag tataacttgg
      601 atttggaagg atgattggat gaaagctatt acgcctgata tgacaacaaa atttaacaaa
      661 cagtttcaag caaatatcaa acagcaggca ttgcagcggg cagttgtgaa gaacggaatt
      721 actgcttctg ctgaaaatgt ttcagcacat gtggagaata caccggtatt ttctatagat
      781 ttggctacgg ggaaagtggc taatcaaatg caatcggggc gttgctggat gtttgctgca
      841 ctgaacacat tccgtcataa gatgctgaat caattccatt taaaagagtt tgaattatca
      901 caaaattaca cttttttctg ggataaatat gaaaaggcaa attatttcta tgaaaacatc
      961 attgcaacag gaaaagaggc aatcgatagc cggaaagtag ctttcttact cgctactcct
     1021 caacaagatg gcggccaatg ggatatgatc gtttcattat tccaaaaata tggcgttgtg
     1081 cctaaatcgg tgatgccgga aagtgcgaat agttcaaatt ctcgagattt gaataactac
     1141 ttgaacaaat tactgcgtaa agatgcagta gtgttacgga aaatggttgc tcaaggagaa
     1201 acattagacg ctattgaaga aaagaaagaa gaaatgctag aaagcattta taacttctta
     1261 gctatctcat taggaacacc tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag
     1321 aactatcatc ttgaccgagg actgacacca caaatttttt atgataaata catcggtgta
     1381 aaattagatg attatgttag cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt
     1441 tcttatacag tagagatgct aggaaatgtc gttggcggga aagaagtcaa atatctgaat
     1501 gttgatatgg aaactttcaa aaaattagcg attgctcagc ttgaagaagg agaatctgtt
     1561 tggtttggtt gtgatgtagg tcaatcttct actagaacta gtggcatcat ggccttagat
     1621 gcttattcaa tggatgatct atttgatacg gattttacaa tgactaaagc tgaacgttta
     1681 gattttggag aaagtctgat gacccatgcg atggtattga ccggtgttga cttagtagac
     1741 gggcaatcaa caaaatggaa agtcgaaaat agctggggcg aaaaagttgg aaagaatggc
     1801 ttctttgtta tgagtgatgc ctggatggac gaatatacgt accaaatcgt tgtaagaaaa
     1861 gatttattga cgaaagagca attaaatgcg ttcaatgaag aaccaattgt tctatctccg
     1921 tgggatccaa tgggtgcttt agcatagacg agtatttcaa tgaagagtat gtagtaaaga
     1981 taaaaagagc tggcttaagc cagctctttt tatctttaaa gtccattccc tgttttttat
     2041 ggtccctgtg ctataattct aaataaagga taaggattgg aaataatcct tttatcatta
     2101 aaaatatgat gacgaagtag aataaaattg ggagtagggg ggaaagtcat ggatatccaa
     2161 actgttttat ctgatctgaa attaaatggt aaagtgattc cagttgttgg tggcgatgtt
     2221 aatcagacct atcgtataaa aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat
     2281 gtaaaaaaag gattttttga agcagaagta gacggattaa aagaactgtc agcattcgta
     2341 cgtgtaccgg atacgtatat gctaggtgaa acaagtgaag gcgcttattt gttaatggaa
     2401 tggatcgagc cgggaaaagg ggatcaacga gatcttgctg cagctttagc taatctccat
     2461 cagcaaacag cgccacaatt tggttttcgt aaagataact atttgggtac acttgttcag
     2521 aaaaattctt ttgaagaaga ttggtggact tttttcttta aagatcggct tgaatcccag
     2581 atttctttgg cagaagaaac gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga
     2641 tttaaagaac gtgtattgaa atcggttgaa ccaaaaaaga tcactccacg cctcttgcac
     2701 ggagacctgt ggagtggtaa tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac
     2761 ccagctgttt cttatgggaa tcgggaacaa gatattgcaa tgagccagtt attcggtggt
     2821 ttccgaccgg aatttttaga tgcttatcaa actatatttc cattagaaaa gggatggaaa
     2881 gaccgactgc cgatttacca gttatattat cttttggcac atttgaatat gtttggagaa
     2941 agttacggtt cccaagtaga tcaattacta gaaaattttt aagaggaggg cgtgtttttg
     3001 gcaaaaaaat ataccaaaaa gcatgaagtt tcatattatg agtgtgacat caaccaaacg
     3061 atgacatttc cttcgatgtt aggagtagtt atcaaaacgt ctgaagatca aagcgaccag
     3121 ttgggaagag gcagtgaatt cgtcaatagc tttggactta cttgggtcat cacgaattat
     3181 tccatggaga ttaatcgtct ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc
     3241 atggagtaca ataagttttt ctgttatcgg aacttctgga tccatgatga agatgggaaa
     3301 gaattagtca aaatagaatc tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc
     3361 agtgttaatg aagagatcat tgctcctttt gagagtgaga agatcaagaa aatcaagcgt
     3421 caggaaaaaa ttgaaaagat tgattcgggc ttcatgttgc catatcgtgt tcgtttttat
     3481 gatatcgata gtaatcagca tgttaataat gcgatgtatt ttaattggat cattgacgta
     3541 ttaggatatg actttttgac tacgcatgtt ccagaaaaag taattattcg atttgataaa
     3601 gaagtagagt acgggaatga gatcgaaagc cattttgaac aaatccctat cgaaaatggt
     3661 gtcaaaacaa aacatgaaat ccgaatgcaa gaacagctgt attgtgaagc aaatattata
     3721 tggaaaaata aataaaaaag aggttgtgac agaagggggc aacttcaaga aataagaaga
     3781 aatttacgaa aattgtttct caaatttttg taaattttcg acttagtttt cgaagaagct
     3841 atttttgtca cagcctctct ttttaagttt agctattaga taatgatgtt tagttttgga
     3901 taaacgaaat caattcttca cccgacaaac catagtttac agcaatcaat agttttaaac
     3961 gtgctttttg actattaata ctgttacaga aaataatccc cattttttct agttcttttc
     4021 ccccgccttc ataatcataa acaggctctg cgattccatt gaaacaacga gaaacaagta
     4081 ctacagggat tttttggaca agaagctttt ccaaagcagc taaggcttgt ggagggagat
     4141 tccccgcacc taaagcttcg attaccaaac cgtctacttt cgtatgggcg atggcttcca
     4201 ataagtcccc ttgcatccca gcatatgctt taacgatcgg gatagtccca tctacagcgg
     4261 aaatgtccag tcgctttgtt tctaaaagtt cctgcaagaa taaaattcga ttcttagtta
     4321 ctaagccgat gggacccaat gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct
     4381 ttgtcacgta gcgtgcagaa tggatctcat cgttcatgac caccaaaacc cccttatcta
     4441 gcgcttcttc acaacttgcg acacgaatcg cactttcaaa attataaagg ccgtcacttc
     4501 caagttcatt acttgaacgc attgccccag tcaaaacaat tggtaatttg ttgccaatcg
     4561 ttgtatcaag aaaaaaagca gtttcttcta atgtatctgt accgtgtgtg atcaccactc
     4621 cggatatacc tgatgcataa gcggacaaga tacgttcttt taataccaac atgtgagaag
     4681 gcatgatgtg tggtgaggga agattgaaga tatcttcaac taataattct acatgcttgg
     4741 ggattgtgat ttctgcatct aataatgggt ttgccacatc aggactaaca ccgcccgtga
     4801 gcatgttttc tttcatggcg atcgttccgc cggtgtgtag tacaagtatt tttttcattt
     4861 tacagacttc cttacgaata attttgcata gccgttaaat cttattgtat actatataaa
     4921 agagttttgc tatcagtgaa gaataacgaa tagcctaact tatcataaaa aagctgacct
     4981 tattaaaatg gaaaagactc aaccttgacc tcctaataca atggagtatc tggttgagtt
     5041 tcttttacaa gaaagcagcc aaggaaatca atcacgaagg tggccggaaa tgatcagaat
     5101 cggattaaca tcgtttaaag aacatgacaa attaactggt aaaaagaata acactcttta
     5161 tgaatatgca gggtttctac ccttagtgga attagatact gcctattatg gaattccccg
     5221 aaagtcctca gttgagaatt ggataagtga agtacctgaa tcatttcgct ttgtagtcaa
     5281 agtctatcaa ggaatcagcg gtcaaggaga ctggaagcct tattatcaga ccgaagaaga
     5341 gatgattgcg cattttttag atgttatgtc gccaatggta gatgctggaa agatgttttg
     5401 ctttttagtc caattcccct catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg
     5461 caaacttaga aagtggtttg agggttatcc tgtttctata gaattgcggg attattcttg
     5521 gtacagcgat gaatttcttg aacgtacaag aaactttatg tctagagccg ctttttcttt
     5581 agccatcatc gatgagccac agttgctgaa tacaacgatt ccttttgatc catttgtcac
     5641 caatccgaac tttgctcttt ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa
     5701 tgataaagag tggcgtaaaa aaagaacgtt atatcgttat tcagaaaaag aactgcaaga
     5761 actagcacat gaagtcaaag tagttgattc taagacaaaa gaggtaggtg tgatttttaa
     5821 taataattct ggtggtgatg cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat
     5881 caagccagat ggactcaatc caacccaaat ggatctgttt taagggctta ttttattcaa
     5941 gaaaggggcg aagtctatga tcaaagctat tttttttgat attgacggaa cattggtcac
     6001 caatcgttca aaagcgttgg aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg
     6061 cattttatgc ggtgtagcaa cggggcgcag cccagtaaag atcaaagaaa tcattgatga
     6121 attggaatta gatatgtatg tcgtgtataa tggtcaactg gtttttactg cagatcgcac
     6181 gattatcgac cacccttttg aacaaaaagt attggaacat attgttgaat ttgcagatga
     6241 gaaccatcga caaatcgttt ttggtgcgag aaatcggtta gatggatcaa cgacgatgct
     6301 attaggacaa tcgattttta tcaagcgact agtcagcttt ttaccgcgta aattcccggt
     6361 acgtctgatg aaaaaaatcc tccaggtttt tagccctcac cgacaaaagg atcgttatga
     6421 aacgttagag atattaaaag aaccaattta tcaatgtata ttgctcagtc cggagtcaga
     6481 acagaaaaag ttagaacatc gctttcccga gtgtacgttt cagcgttcaa atacgtatac
     6541 tgtagatatc atcccaaaag gtggttcaaa attactaggc atccaagctt tcgcaaatgc
     6601 tgtcgaaatt gaaatggaag aaattatggc attcggcgat cattacaatg atatcgagat
     6661 gctgaaaggt gtaggaattg gagtggccat ggggaatgct cagatcgaag taaaacatgc
     6721 tgcagacttt gtgactcaat caaatgaaga agatgggata tacgaaggac tgaaacactt
     6781 cggtctgatt tattagtcaa tgaaagaagg aaaaaatcag tatggaagca gagacacctt
     6841 ttcaaaaagt agaaacattc cacaaaactt ttgatccaag aagaccagag atacccacgg
     6901 cttttacaga aaatgaagct catacacgtg ctgcgttcaa aatagaagaa cttgttgaat
     6961 ttgttgcagc tacttcacct aataaagaac aactgcatga agctgttatg ctgctgcacg
     7021 aagcattaga taaagcagaa gaaaaaatac ttggtaaaac acagtgggga agtgatccgc
     7081 tggttgaaca agtagatgca ctgacagata tcctttactt tacttatggt tctttttcat
     7141 tactaggaat agtgcctgat cgaatattcg aaatcgtcca tcaggcaaat atggggaaat
     7201 tatttccaga tggaaaacct cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa
     7261 actggcagat ggaatatgct ccagaaccta aaatcgcggc agaaatccaa cgtcagattc
     7321 aagaagcgat tgctaagaag aaatccgctg atataggcat gtgcacaaat gaaaaagagt
     7381 gatcagagtt tcactatttt tgttatcttt atacgatgga aaaacaaaag attttcgttt
     7441 aagttagtga atgcatgaaa atagtatgct ataatctaga atagatgtct tttgcacgag
     7501 agacgtatta tttggattta tgccaagcag aacgccatct gttgcataaa agatgcatac
     7561 tgaaaacgga ggaaccataa atgggaaata atgttatcat cggaatcgtc attgcaatca
     7621 ttatagtcgc cattgtcgtc tttttggtta gttacttgat gaagaaaaaa aaccaagatc
     7681 gattggatgc cttagaaaaa cgcaaggaag aattatttga cttgccagtg attgaagaag
     7741 tcgatgatgt taaaaaaatg catctggttg ggcaaagcca aaataccttt cgtgaatgga
     7801 gtcaaaaatg gaatgacata tctacttcct cgttcgctga actagaaagt caaattttcg
     7861 aagtggaaaa cctcaatgaa agttttcggt tcttaaaagg aaaaaaagca gttgttgaag
     7921 cagaagctac aatggaccaa atggagaaac aagtgacgga aatccgtgaa ggtctaaagg
     7981 aattgcggga aacggaagaa cgcaactcac tagctgttca gcaggcactt gacgtctatg
     8041 aagaactgaa agaaaaagtc agagaagacg agcatgaata cggaccagct ctttttgaat
     8101 tgcaaaaaca gatcaaagtg atcgaaaaag aatttacgca gtttgtgact ttaaacactt
     8161 ctggagaccc tgttgaagcc cgcgaggttt tggataaagc agaaaaacat acgtatgaag
     8221 tggaagctct gatgaaaaaa attccaccgc tgtatgagga gctccatacc actttccctg
     8281 aacaacttga agaaatcagt gatacttacg agaaattgat ggaagaacaa tatgtattcc
     8341 cagaagaaga tcttgctgaa gatatcgcaa aagtaagtcg aagaattgaa aattcattag
     8401 ccaatcttga aaaaacagaa gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt
     8461 tgattgattc gttatatgat atcttagaac gggaaatgga agctcagaga tatgtaaaaa
     8521 ctaaccaatc aacgattgca gagtatatca aacatacaac aaagaataac cgtcaattat
     8581 tgattgagct ggatcacaca tctcaaagct atacattgaa ccacaatgaa attggccgtg
     8641 tacgaggttt ccaaaccgaa gtggaagaaa tggaacgtca aaatgaacag atgattccac
     8701 agatccgtca acacgaaatt ccttattcag aagtgcgcac gttctataaa acagtcttca
     8761 aagtcttaga agatatcgaa aagcaacaag tagagatcga cgactcatta catgaattga
     8821 gaaaaggcga aaaagaagcg caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt
     8881 tgaaacgttt tgtagaaaaa caacgtttgc ctggcttgcc gaatgattac ctagaattct
     8941 tttttgttgc tactgaccgt attgaagaac taagtgttgt cttgaataaa attcgagtaa
     9001 acatggaaga ggtcaatcgt ttagttgctc tatgtgaaga agatctagaa ttattagata
     9061 agaagacaca tgatttagta gatgcagcag cattaacgga gcaaatgctc caatatgcaa
     9121 atcgttatcg tcatacacac gaagaagtga gagaagcaat tgataaaagc tactatttat
     9181 tcaataaaga atatcactat caagaagcat tagacgaaat cggtacagca ttagagcgag
     9241 tagaacctgg tgctttcaaa cgcatcgaag atttttattt taatcatcca gacttagtat
     9301 aaagcaaaaa agttagtaaa ccagcaagag caagacggat tattgtcttg ttcttgtctt
     9361 tttttgtata tttgaaaatt gaaacaagtg aaaatttcac ctgctcttga aagcaccttg
     9421 gacaaccgtt ataatagaaa agaatttcaa aaaagagggg tttcttatga tatattttga
     9481 taacagtgca acaaccccaa tctttccgca aagcttagat gcatatgtaa aaaccagtca
     9541 gcggattatt gggaatccat caagcttgca tgatttaggc aatcaagcta accggttgtt
     9601 gcagcaagca agaaagcaga tcgctgattt gatccatgta gaaccagaag aaatctattt
     9661 tacttcaggc ggaacagaag gagataactg ggtattaaaa ggaacgatga tcgaaaaaag
     9721 agaatatggg aatcatatga tcatttcagg agtcgaacat ccggccgtca gcgagacagc
     9781 agaacaatta aaagaattag gaatcgaagt aagtatcgcc ccagtggata aacgcggatt
     9841 tgtacgtgtc gacgaattga aagaattaat aagaaaagag acagtattag tctctgttat
     9901 ggctgtgaat aatgaagtag gatcgattca gcccatccag gaaatcagtg atcttttgga
     9961 tgattttcct aaaattcatt tccatgtaga tgctgttcag gcgattggaa aagtaccatt
    10021 ctcgcaatgg ttgacagaaa gagttgattt tgcaacattt tcagctcata aattccatgg
    10081 cccacgggga actggtttca tttattggaa aaagggtcgt cgtttagcac cattactgac
    10141 aggtggaggg caagaaagaa atcagcgaag tgggacagaa aatgttccag gtattgtggc
    10201 tatggttaaa gctttacgtt tatcattaga gataaaggaa caaaaacctg agcagacaca
    10261 acgcttaaaa gtagctctaa tcaatgcgtt gaagacgtat gagaaagtga cgattttttc
    10321 agaaggaact gactatgcac cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga
    10381 agttctcgtc catgccttag aagaaaaaca aatatttgtt tctactacta gtgcctgctc
    10441 ctcacgtaaa catatggcaa gcagtacatt gcacgcaatg catgtaccaa atgatctggc
    10501 tacatctgct gttcgaatca gcttagatga aaacaattcg cttgcagaag tcgagcaatt
    10561 tatgattatt ttcaagcagc tttataagaa attttctgca atcaattaaa agaggagtgt
    10621 tcttgtgaaa tacagtgaaa ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa
    10681 tcgaaaaagc tttatcatgc aactagcaca aaatgtcaga caagctttga aagactttcc
    10741 agaaatcaaa atccatgctg atcgggaccg gatgcatctt ttattgaatg gcgcagacgg
    10801 gcagctagtt attccaaaac tagcaaaaat atttgggatc caaaatttct ctccaagtat
    10861 tcgtgtagaa aaagatgtcg aagttttgaa acaagctgtt caagacatca tgaaagaaat
    10921 ttatactggg aaagaaacat ttaaaattat cgctaaaaga agcgatcacc aatttgaatt
    10981 gacaagtaat gaattgaatc aaacattagg taatgctgtt tttgatattt tcccgcatat
    11041 ccaagttcaa atgcgtcaac cagatattcc attacgtgta gagattcggc gtgatggcgc
    11101 ttatctatcg tatgaaacga tcaagggagc tggaggattg cccgtaggaa caagcggaaa
    11161 aggaatgctt atgctttctg gggggattga ttctcctgta gcagggtact ttgccatgaa
    11221 gcggggagtg gaaattgaag ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc
    11281 actgcaaaaa gcaaaagatc taacagccaa actagcacct tatgtgggaa cgatccattt
    11341 tatcgaagtg ccatttaccg agattcaaga agaaatcaaa aagtcagtgc cacaaggata
    11401 ttggatgaca attactcgtc gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg
    11461 tcatggttta gtcattttga atggtgaatc attaggtcaa gtggcttcac aaactttgca
    11521 aagtatggtg gctatcaatg aagtaacgaa tacgccaatc attcggcctg tagcgacaat
    11581 ggataaactt gaaatcattg aagtagcaga acagatcgat acatttaatc tttctatcca
    11641 gccttttgaa gattgctgta cgatttttgc tcctccacag ccaaaaacac gccctcaact
    11701 ggaaaaggtc catcaatatg aagagcgttt agcaatagat gaaatgatcc aacgagcact
    11761 agctggcttg aagatggaag agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt
    11821 ttcagaattg ctataagagg ttatgaaaat agtgttttga ccagagtatt agagaaacaa
    11881 aacgaaaaaa tagctccatg tattttaccg tttttgaact aatgtcgaag attagctgat
    11941 tgaataccat ttatcaagaa gttatggaaa agccggttct tttttactaa ctgaaaaata
    12001 aaattttatt tagagatcaa gctacagttg ataaatgata taaaaaagag aatttcccat
    12061 gaaatagcga ggttgtgaca gaagtgggca gcttcaagaa ataagaagga atttacgaaa
    12121 attgcttttc aaatttttgt gaaatttcgg cttatttttc gaagaagtta cttctggaac
    12181 accgtgtatc agaggttg
//
LOCUS       DAGCYL010000077        11995 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.078, whole genome shotgun sequence.
ACCESSION   DAGCYL010000077 DAGCYL010000000
VERSION     DAGCYL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.078"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(56..643)
                     /locus_tag="MB216_001581"
     CDS             complement(56..643)
                     /locus_tag="MB216_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526421.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            818..2140
                     /locus_tag="MB216_001582"
     CDS             818..2140
                     /locus_tag="MB216_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4526422.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2295..3071
                     /locus_tag="MB216_001583"
     CDS             2295..3071
                     /locus_tag="MB216_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBT4526423.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3064..3633
                     /gene="rnmV"
                     /locus_tag="MB216_001584"
     CDS             3064..3633
                     /gene="rnmV"
                     /locus_tag="MB216_001584"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBT4526424.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3626..4510
                     /gene="rsmA"
                     /locus_tag="MB216_001585"
     CDS             3626..4510
                     /gene="rsmA"
                     /locus_tag="MB216_001585"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBT4526425.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            4961..5590
                     /locus_tag="MB216_001586"
     CDS             4961..5590
                     /locus_tag="MB216_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526426.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5714..6823)
                     /gene="ugpC"
                     /locus_tag="MB216_001587"
     CDS             complement(5714..6823)
                     /gene="ugpC"
                     /locus_tag="MB216_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBT4526427.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7032..7454
                     /gene="mgsA"
                     /locus_tag="MB216_001588"
     CDS             7032..7454
                     /gene="mgsA"
                     /locus_tag="MB216_001588"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBT4526428.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            7777..8313
                     /locus_tag="MB216_001589"
     CDS             7777..8313
                     /locus_tag="MB216_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBT4526429.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8353..10116)
                     /locus_tag="MB216_001590"
     CDS             complement(8353..10116)
                     /locus_tag="MB216_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4526430.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10119..11834)
                     /locus_tag="MB216_001591"
     CDS             complement(10119..11834)
                     /locus_tag="MB216_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4526431.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3741 a         2218 c         2448 g         3588 t
ORIGIN      
        1 aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact
       61 tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca
      121 gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat
      181 agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa
      241 accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat
      301 aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata
      361 aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat
      421 acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca
      481 aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta
      541 atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg
      601 ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg
      661 tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga
      721 gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga
      781 aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga
      841 aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt
      901 cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg
      961 gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc
     1021 aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag
     1081 ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc
     1141 gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt
     1201 gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg
     1261 tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat
     1321 atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa
     1381 agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga
     1441 cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc
     1501 taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt
     1561 ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc
     1621 atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc
     1681 ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc
     1741 tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt
     1801 tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc
     1861 tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt
     1921 tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc
     1981 actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac
     2041 gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc
     2101 agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag
     2161 aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt
     2221 cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa
     2281 aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg
     2341 aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg
     2401 tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata
     2461 tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa
     2521 ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg
     2581 attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg
     2641 ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca
     2701 cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca
     2761 gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg
     2821 gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg
     2881 aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc
     2941 gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc
     3001 cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg
     3061 aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga
     3121 aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa
     3181 gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat
     3241 cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa
     3301 catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag
     3361 catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc
     3421 caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg
     3481 gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac
     3541 ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa
     3601 gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag
     3661 aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt
     3721 tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac
     3781 gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc
     3841 aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat
     3901 gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac
     3961 agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta
     4021 ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact
     4081 agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc
     4141 ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt
     4201 accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag
     4261 aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc
     4321 ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga
     4381 tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg
     4441 tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa
     4501 tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa
     4561 taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttattaccgc
     4621 agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa
     4681 gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta
     4741 cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc
     4801 acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt
     4861 tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag
     4921 tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg
     4981 aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc
     5041 taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc
     5101 gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt
     5161 tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgcgttgat
     5221 tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt
     5281 tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga
     5341 aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca
     5401 aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt
     5461 tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt
     5521 atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac
     5581 gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc
     5641 tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct
     5701 aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc
     5761 ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat
     5821 ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa
     5881 gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta
     5941 cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac
     6001 ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat
     6061 ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa
     6121 agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt
     6181 ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat
     6241 cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac
     6301 gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt
     6361 ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag
     6421 cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac
     6481 gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa
     6541 aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt
     6601 taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt
     6661 ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa
     6721 cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat
     6781 tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt tccacacttt
     6841 cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa cttcaatgtc
     6901 atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc tttaataaat
     6961 atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag
     7021 gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga
     7081 aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga
     7141 ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt
     7201 taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt
     7261 ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt
     7321 tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa
     7381 gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc
     7441 cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta
     7501 gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata
     7561 aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg
     7621 ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg
     7681 gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat
     7741 gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg gttagacgca
     7801 cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta
     7861 gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc
     7921 attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg
     7981 gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga
     8041 gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct
     8101 acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg
     8161 taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata
     8221 tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa
     8281 attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa
     8341 gactgcttgt ttttattgtg ctgtgaattg tgcttgatag agccgtcggt aataaccatc
     8401 tttttgaagc agctcatcat gggtgcccat ttcgacaata gctccctgat ccatcactaa
     8461 tatctggtcc gcatttttga ttgtggacag gcgatgggca atcacaaaac tggtttttcc
     8521 ttccatcatt gctaagaagg cttgttggat ttttttctct gtcaatgtat cgactgagct
     8581 ggtagcttca tctaagatca acataggggg attactgatc atcgttcgag caatcgtcaa
     8641 cagttggcgt tgtccatctg atatctttaa cccttgaccg ccaatcttag tatctaattt
     8701 ttccggcaaa cgttctacaa attcatacat ataagaggct tttagggcat cattgatttc
     8761 tgcgtcactt gcttccggat tcccaaaacg aagattttct cgcaatgtac tgtcaaatag
     8821 ccaagtatct tgtaacacca ttccaaaact tttacgtaat gtatcacgtt ggatagagcg
     8881 gatgtcatgg tcatcgactg taattgttcc ttttgttgtg tcataaaaac gcatcagcaa
     8941 attaacaagt gtggattttc cggcccctgt ttttcctact atagcaatgg tttgacctgg
     9001 ataagcagta aaattgaaat cttcgatcaa acgctgcttg gggtcataag aaaaagaaac
     9061 atgatcaaaa gagacttttc ctttgatatt tttgggttcc aaggcatagg catctggcaa
     9121 atcaggcttt tcttctggct gatcgatcaa atcaaatgct cgttctagac cagaaaaagc
     9181 ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa actgacttga
     9241 gtagatcgta aagctggaaa tcacaccaac agtgattgac gaaccactct tcaatgccaa
     9301 taggccgcca actaaaccaa tcgataaata agcaaggtga tctacaaaac gagaagacgg
     9361 gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt tcatatagat cagcatttag
     9421 cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa gctttcacga ttttctggtt
     9481 accgattcgt tcggtcacaa agcccgagat atccccaata atcctttgct gtctatcgaa
     9541 atttgtttgt gaagcacgag caacgatcca actcacaagg aaaataattg gtgtagaaca
     9601 aagaacgacc aatgtcagaa gtggacttag acgcatcata aaaataaacg aaagaatgat
     9661 gattgccact ccagaaaata attgattgaa ggccgcagaa attgcaatgg aaatattgtc
     9721 catatcattt gtaaatcgac tgacgatatt tccgtgggag ttttgatcat aataacgaag
     9781 tggcaatcga ttcagatgag caaatgcatc ttttcttaat tttgcaacgg ataagtaagc
     9841 taaccgattg cttaacactt ggatgagcca ttgactgact acgttgatta aaacgatacc
     9901 accgaataga ataagtaaat ggaacagtcg attgaagcgg acctgtcctt ctccaatcat
     9961 ttgatcgata ctttgaccaa tttgtaacgt catcacaact gttgcaatcc cattgatgat
    10021 tcctagtatc acagctagcc aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg
    10081 gcggagcgtc tgcaaggata atttttttac tttcataact attcttcctc ctgtgatgcg
    10141 acgatttcct gatattctgc tgattgagca agtaattctt catgggtacc ctgagcagca
    10201 atcttacctt gatccatcac taagatatga tgtgcttctt gaatagagcg gacacgttgg
    10261 gagataataa tcactgtact gccaagataa gtctgtagcg ccttacgcaa atccaagtct
    10321 gtttgataat ccaacgcact taaagagtca tctaaaatca gtaatgctgg cttggcaatc
    10381 aaagctcttg caatcgtcag tcgctgtttt tgtccgccag agaaattttt cccattttcc
    10441 atgacttttg tatctaaacc atttggaagc tgacggacaa attcttctgc ctgagcgatt
    10501 ttcagagctt cccaacattc tttttctgtg gcgtctttct taccccattg aaggttttca
    10561 cgaatagtcc cggtaaatag tacagaagtt tggggaacaa gagcaatttg ctgacggaga
    10621 tgatccagct gccaagaacg aacatccatt ccgtccatta taactgtacc ggaactgaca
    10681 tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt tggaccaatg
    10741 attccaagga ctgtatttcg cttcagctga aatgtaatat tttggagaga aagaccagaa
    10801 tcaggagtat aacggaagtc aacatggtca aaagcgataa ttgcttcaga agaaaaatcg
    10861 ggagtagtac tgacctcagc ttcaagaatc gaattttctg tgtctagcac ctctttcacc
    10921 cgatttcctg aagcttcggc acgtgtgaag atcacgacga ggtttgaaac aacgatcagt
    10981 gccaaaagca tttgattcat ataattgatc aaagcaagaa cttgtccttg ttgcaagcta
    11041 ccaatattta ctttgatccc gccaactgtc agcaaacaga tgatcccgac attcatgatc
    11101 aaggttgtag caggtgataa tagggcagag atattggaaa cacgtaaata attgttcgct
    11161 agatcatttg tgcttttatc gaacttctct gtttcttttt ttgtctgcgc aaatgcgcgg
    11221 atgacacgaa caccgctaag attctgacta acgtgttcat tgagctgatc tagtttttct
    11281 tgcacttttt ggtagagagg aacggttttt ttgatgataa aaaataaaat gattgagaaa
    11341 ataggcaata atgctaagaa aaacagacct acttcccagt cgatcacaaa agccatgacc
    11401 actgacccga tacttaaaaa cggtgctctg atcaataaac gaatgaccat tgctaaagct
    11461 agctgaagct gattgatgtc gtttgtcatt cgggtgatca aggtatctgt tccaaaatga
    11521 ttcaactcgt tgtgagagaa agtattgatt tttttgatta gctgattacg taactctgta
    11581 ccaaatcctt gtgaagctac tgaagcatag tattggcaaa tgatagcaca aatcagacca
    11641 atcacagaca tagcgaccat ccataagccc atttcaatga tttttcctcg gtcattcatt
    11701 tttagtccat tatcgatcaa ggaagccatc attaaaggca ggacaagttc gaagacagct
    11761 tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac gatagtcttt tgcataacgt
    11821 aataactgaa tcatatgatt cctcctatta tttatttgtc cacaattgaa aaaacaagta
    11881 ctaccacatt gtagcttagg aaatttagaa tgaaaagaga acaagccttt tcataaatga
    11941 aagttttatt atgaagaagc tttagaaatt gaatagacta ttttttttaa aaaaa
//
LOCUS       DAGCYL010000078        11811 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.079, whole genome shotgun sequence.
ACCESSION   DAGCYL010000078 DAGCYL010000000
VERSION     DAGCYL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.079"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            436..2076
                     /locus_tag="MB216_001592"
     CDS             436..2076
                     /locus_tag="MB216_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBT4526432.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2230..3552
                     /locus_tag="MB216_001593"
     CDS             2230..3552
                     /locus_tag="MB216_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBT4526433.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3614..4045)
                     /locus_tag="MB216_001594"
     CDS             complement(3614..4045)
                     /locus_tag="MB216_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4526434.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(4109..4945)
                     /locus_tag="MB216_001595"
     CDS             complement(4109..4945)
                     /locus_tag="MB216_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBT4526435.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(5135..5437)
                     /locus_tag="MB216_001596"
     CDS             complement(5135..5437)
                     /locus_tag="MB216_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBT4526436.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5463..5894)
                     /gene="ruvX"
                     /locus_tag="MB216_001597"
     CDS             complement(5463..5894)
                     /gene="ruvX"
                     /locus_tag="MB216_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBT4526437.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5891..6157)
                     /locus_tag="MB216_001598"
     CDS             complement(5891..6157)
                     /locus_tag="MB216_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBT4526438.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6411..7136)
                     /locus_tag="MB216_001599"
     CDS             complement(6411..7136)
                     /locus_tag="MB216_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBT4526439.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7502..7572)
                     /locus_tag="MB216_001600"
     tRNA            complement(7502..7572)
                     /locus_tag="MB216_001600"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7538..7540),aa:Gly,seq:ccc)
     gene            7689..8441
                     /locus_tag="MB216_001601"
     CDS             7689..8441
                     /locus_tag="MB216_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBT4526440.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8457..8936
                     /locus_tag="MB216_001602"
     CDS             8457..8936
                     /locus_tag="MB216_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBT4526441.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(9187..10767)
                     /locus_tag="MB216_001603"
     CDS             complement(9187..10767)
                     /locus_tag="MB216_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBT4526442.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10760..11503)
                     /locus_tag="MB216_001604"
     CDS             complement(10760..11503)
                     /locus_tag="MB216_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4526443.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KRLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11679..>11811)
                     /locus_tag="MB216_001605"
     CDS             complement(11679..>11811)
                     /locus_tag="MB216_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBT4526444.1"
                     /translation="NITSISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
BASE COUNT         3598 a         2343 c         2094 g         3776 t
ORIGIN      
        1 caatctaacg atttttttac tgggaagtta tccagggaga agtatgctca aaatgcctta
       61 cacccaaaat ttattcctca atatttttga acagtgaatg tttgaagtaa ctttttagtc
      121 aaaatcagtt gatagggata ttagtgatac atatttcaat aaaatgtgcc aaagaattaa
      181 agctatgaat tattcaggat tctatttatc tatctaacat atcctttatt cttctacttt
      241 tctttcttta gcatataaga gatacgcata gtaatcaagc aaggattgtt gatataaaaa
      301 gaacaaatac cgctattttg atttcttttg actctattac tcatataaat aaaatttatt
      361 atacctgctc tttttgacta tatcctcatc tgactcctat acttcgtttg tattaaaact
      421 taggaggaat cattcatgct tacagggcta aacttattaa ataatccttt tctaaataaa
      481 ggaacagctt ttacaaaaga agaacgggca aaatacggaa tcactggtat gcttccaagt
      541 actgtccaaa cattggaaca acaatctgtc caagcttacg gtcaatactt aagcaaacag
      601 accgatttgg aaaaacgtat cttcttaatg aatctattca atacgaaccg gacattgttt
      661 tataaattga tgggcgaaca tttagttgaa ttcatgcctg tcgtttatga tcctgtcgtt
      721 gcggattcaa tcgaacaata caacgaaatt ttcttgaaac cacaagatgc tgtatttctt
      781 tcaatcgacg aaccagaaaa tatcaaagca agtttgaaaa acgcagcaga tggacgcgac
      841 attcgtttga tcgttgtgac agatgcagaa ggaatcttag gtatgggtga ctggggtgtg
      901 aacggtgttg acatcgctat cggtaaattg atggtctaca ctgccgcggc tggtatcaat
      961 cctgcacaag tcttgcctgt atcaatcgac gctggtacta ataacgaaga attattgaat
     1021 aatccattat acttagggaa tcgtcatgct cgtgtagaag gcgaaactta ctacgaattc
     1081 attgatcaat tcgttcaatc agctactgaa ttattcccag aattgttatt acattgggaa
     1141 gattttggac gaggaaatgc tgcaacgatc ttagaaaaat acgaagataa aatcactact
     1201 ttcaatgatg atatccaagg cacaggaatc gttgtactag ctggtgtatt aggtggattg
     1261 aatatatcag gtgagcaatt gaaagatcaa acgatcttga catttggtgc tggtactgct
     1321 ggtgtaggta tcgcaaacat ccttttagat gaaatgatcc gtcaaggtgt acctgaagaa
     1381 gaagcacgcc aacatttcta tcaagtagat aaacagggtt tattgtttga agatacaaaa
     1441 ggattgactc ctggtcaaat cccattcgct cgtaaacgct cagaattcac taatgctgat
     1501 gaattgacaa acttagaagc agttgtaaaa gcaatccatc caacgatcat gatcggtact
     1561 tctacccaac ctggagcatt cactgaagaa atcgtgaaag aaatggcggc tcatacccca
     1621 cgtccaatca tcatgccttt gtctaatcca acaaaattgg cagaagcaaa agcaaaagat
     1681 ttgattgaat ggacggacgg aaaagcttta gttggaacag gtatccctgc tgctgatgtg
     1741 gaatataacg gtgtaactta ccaaatcggt caagcaaata atgcgttgat gtacccaggt
     1801 ttagggcttg gtttgattgc ttcaacagct acacgagtaa atgctgaaat catttctcaa
     1861 gctagtcgag cattaggcgg aatcgttgat gtaacaaaac caggagcagc tatcttgcct
     1921 ccagtagcaa aaatcactga attctctcaa acgattgctg aaactgtcgc aaaatcagtt
     1981 gttgcacaaa aattgaatcg tgaagaaatc acggatatca aagaagctgt tgaatctgct
     2041 aaatgggttc ctgaatataa atctttagaa gattaagatt gttatagtaa aagaacatac
     2101 agaaactttg tatgacgtct aaaaagaaaa aagcgactgc ggcaaaacag cagccagcca
     2161 actactgctc ttttgccgct tctctttggc ttttttcata cgatacagaa taagaaagga
     2221 tctgtaaaaa tggcacaaaa aacaacaacc gcaactgcag ttgaggaaaa gaaaaaagaa
     2281 gggttttggt caacaaaaat taccggcgta agtttgcccc tttatttgat catggtcctt
     2341 gtcctgatca ttgcaatggc tttaggaaaa ttaccgacga atatgattgg cccaatcgct
     2401 gttttggtca ttttcggtaa tttatttcat tttatcggaa caaaaatccc tatagtcaaa
     2461 agttatttag gtggtggctc cgtttttgcg atctttgcct ctgccgcgat tgctacattt
     2521 gggatcttac ctgagtatgt agtaaaatcc acagaaaact ttgtcagcaa tatgggcttt
     2581 ttagactttt atattgctgc cttgattact ggtagcatcc ttggtatgaa ccgtaatcta
     2641 ttgatgaaag cttctgttcg ttttattccc gttgcactta tttcaatggt cgtctccttt
     2701 tttgccgtag gactcgtagg tatgcttatc ggaaatggtt ttgcaaattc cgttttatat
     2761 atctcattgc ccgcaatggc tggtggtgtt ggagccggag ctgtaccttt atcaacgatt
     2821 tatgcaaatg ttttaggtac ggacgcttct tctatcattt cccgcctgat tcctgcgaca
     2881 gcaatgacaa atgtcctagc tatcattggt gcagcgttag ttgctcgtgc gggacaatca
     2941 atgccaaaat tcaacggtaa tggtaaatta atggaaaaag gcgaccttgg cgatggaaaa
     3001 cctagaaatg taaaaccttc cattccacaa ttaggtgtcg gtttgatggt atcattgact
     3061 ttcttcatct taggacaaat ctgtaactat tttgttccta aagtccatgc ttacgcattc
     3121 atgatcatta tcgttgttat ctgtaaaatc gcaaacctat taccagaata ttacgaagat
     3181 gcagctatca tgttcaacaa cttgattgtc aaaaacttga cagcagctgt tcttgccggt
     3241 atcggtattg cgttattgaa tctaaatgta ttggcttctg ctttgacatg gcaatttgtt
     3301 gtcttatgtt tgacaagtgt tatcgcgatt tcagtagttt caggatttgt cggtcgtttg
     3361 tttggtttat atccaatcga atcaactatt actgctggct tatgcaacaa ctcgatgggt
     3421 ggtacaggaa atgtagctgt cttatctgca gctaatcgga tggaacttat tgcttttgct
     3481 caaatgggca atcggcttgg tggtgccatc gttttgatca tctcaggttt cttgatgcaa
     3541 ttactttctt agtcaaaaaa aatacctgat catcaagtat cggcttgatg accaggtatt
     3601 ttttgataaa aatttacagg attttcaaaa atcttttttg cattttatcg gaatgataac
     3661 cgatgcgttc ataaaatcgg tgtgcttcta ctctgtattc tgcagaatta agtcttattg
     3721 ctgatattcc tcgtcttttc gcttcttcta cgccagtcat caaggccttt ccgattcctc
     3781 ttttttcagc atgtttagac acagcaagtg ccataatatt gaacatcgga tcgctgaaaa
     3841 tcgattcata ggactccgct tgtacatagc cagctgcttt tttagaagta ggatcttcat
     3901 agaccaagaa aaaatttttt tctggctcct tcaacagttt cttcatttgt ctcaccgtaa
     3961 cggttaatgg tacatcatat cctaattgtt ccgcattgat ttctcgtagt gcttctgcat
     4021 ctcctaaacg catttcccgc aacatccaaa tcaccagcca atttttattt ttttaaatga
     4081 atttttttat cacagtatac tccttttttc acttaaaaaa agacttttca gtgattttga
     4141 cttgcttgaa tccagatcct tctaatgttt gcaagatgac gaaagcattg taaagattga
     4201 tcaaaagaac cacgatgaca atcgcttgaa aaataaaggt cacttttttt ggagacaaaa
     4261 ccctgctaaa tcttgctccc cacaaacctc caataacagc agcggggata acataaaaca
     4321 acatagataa atcaaagcgc ataaagctac ttgttaaagc aatcgttacc aattttgcca
     4381 gttgagaaaa gaaaatcgta gatagagaat atagtgttgc ttcttttatt ggcaatgcaa
     4441 acatcagcat caacaaagaa acattgactg gtccaccacc aatacctaaa aaactagcta
     4501 aaaagcccag aaccccccca catataaggt accaagtcca tgacgtcaaa tgaaattttg
     4561 gctgatgatg cttggtgtag aagaacgcaa aaaccaaggt caataccgtc aaaaaaattt
     4621 ggatcagctg cacatgcttc tcgttttcaa ataactgtaa aaaaacttcg aatacaatgt
     4681 tgccgactac tcctccaaca acagctccac tagataccca accgacaatc tgccaattca
     4741 gtgatttccc gcttgatact tgtcgcacag tcgaaacaat ggacatcgtg aataccgcaa
     4801 ctgttgagta aaaagaaatc cctgcaacag aatgggcgcc gataagatca agaatcggtt
     4861 tgatcaacac gcctccaccc atcccggaaa tagcgccaac tgtgttcgcc agaacgatca
     4921 ccacaaaata aatcagacca atcatcttgt atttcctttc tcttgtttat atcttttagc
     4981 gattgttact cttttctttt attatacagt aaaagaaaca aacgaggctt cgaaagcttt
     5041 tctacttttt aaggaaattt agtgctaaac gaaaaaaccg ggctctactt ttcagagagt
     5101 ccagcttttc ttacacctta tcgattcttt tgttctattc ttcttccgcc atgaatgtat
     5161 tgaatacttc ttcgatcata tcccattctg cgtctgtttc gatttgttct aactcgcctt
     5221 ctgttccatc ttcgttttcg atgtatgcat aagcttgtaa ttctacatct tcatcttctg
     5281 aaacaccagc tggatataaa agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg
     5341 ttagcaagat ttcgtataat gtttcatttc cttggtcatc caccaatgtg atgtgttcgt
     5401 gtccttcgtg ttcgtgggaa tggttatgtt ctgtcataag attacctctt ttctttttat
     5461 tattaaaata attgcttacc tgagccgtct aaataatttt gtaaaatcat cacagctgct
     5521 aatttatcga tgactttttt tcgtttcgct cttgaagtat ttgcctgctc tactagcata
     5581 cgttctgcct gaacagtggt tagtcgttca tcttgataat caaccggcaa ggagaatagt
     5641 tctttgatct tctctccata tgacatggaa gcttctgccc ttggtccgat ggaattgttc
     5701 atattcttag gtaatccaac tacaaaacgg tctacttcgt actgcgccac taattccttc
     5761 aaccgatcaa aaccaaactc ttcttctgcc tcattgattc gaatgatttc tactccttga
     5821 gctgtccagc caaagggatc gctcacagca acgcctactg tcttagaacc gacatccaag
     5881 cccattactc tcataaattc actccatgat ttgagaggta gtctttgaca attacttcca
     5941 tgatttcatc gcgttcgtga cggcgaatca aatttcgtgc atcacgataa cgaggaatgt
     6001 aggccgggtc tccagaaagc aagtagccga caatctgatt gattggattg tagccttttt
     6061 cttccaaagc ccgataaaca atcgctaatg tttcgctcac ttcttttttg cgactatcgt
     6121 caaaaccaaa acgtacggtt tcatctgtaa aacccatgat tctacacctc tttcccgcaa
     6181 ggtaattcca gcttaccttc tacaccctta ttttactcga agaaagggaa aactacaaac
     6241 aaatattcca aacttctgaa taaattaaga aacacgattt gtctgatttt tccttttttc
     6301 tgtggctttt tcaaaagccc tctttccata aagtatacgc tatatttctt tgaaagtatg
     6361 tttttacaca attttttcat aaagttcata aaaaattcga attgctgcta ctaatttttc
     6421 gttttcgttg cttgccctgt ctcataattg atggtgtagc catcttgaac attgtagata
     6481 aagacattga attcaatttt tttgtcttca atgctttctg cttccaactg cacacctctt
     6541 gctaccagtt catttccctc gaaatgagga gtgacacgat agcgtacatg atgatttgtt
     6601 tccttgatat atgccgcaat atcattttcg tgggcaagca tgtacggagc attcaacgaa
     6661 cgtgtccctg tcattaagtt tttaatgtta ttattttgac cagtcaactg gaaaccaatc
     6721 aaatggctgc ggttgtataa ccattgtccg tcacttaatt tcttttgttt ccaacctgtc
     6781 ggatcaatat acaaaggttc tctcttttca gtaggaaaca agtcttttcc caacatcgca
     6841 ttggcttgtc ctactctgtt caatttgtct atatttgaat agctttccca tgtccctttg
     6901 gaaagactaa gatcctcttt tgtaaactcc ggctgattcc cattgatttc tatttcttga
     6961 tgttttccat catattctgg cacatccgct acttgagaag cttgcggtgt ggaatcaaaa
     7021 atggttgtaa tttgttggaa gatatctgaa tctttttgtg tggtggaaat accgagtgcc
     7081 cctgcaatca cagcagcgac gatccccacg atcgccttgg ttgttttttt gctcatttct
     7141 ttactccttt actaacagtt ccctaaatct taacatatgt tcccctataa ataaatactg
     7201 gaaagaaata gaaacagtaa aaaaataaaa ccagttttta gacggcttaa gaatacaaaa
     7261 aaagagacgg attcatcgtc tctactcaat cacaaaacac tatttacaag gtatttatta
     7321 tcattcaaaa aatttttctt aatactcaaa actaaacggc ttctgtacga cctcttggta
     7381 aacagtgttc aaaagcttgc atctgcggta ataagtccaa ccaagtcaaa aacataagaa
     7441 gcttttttgc ttgactattc ttattacttg atgcagggca gctttttcac agcctctttt
     7501 aagcgggtga cgggaatcga acccgcgaca tcagcttggg aagctgggat tttaccacta
     7561 aactacaccc gcgatacaaa aacagtatac gcctctttca ctccttcctc aagttttttt
     7621 attctttacg cttccgatat gttttgtagt aaaataaaac taatcaatca attattggag
     7681 aagatgctat gacgggaaaa gaattagatc tgttgctgga cacttgttta ctcgctggga
     7741 aaatcatgat ggaaagcaat gcggagatgt atcgggtaga agatacgatg agtcgaattg
     7801 ctttagcttc tggaaatttc cgtttggtca gctatgtcac tcaaaccggt ctctttattg
     7861 gtcttgatcg tacctcgacc atccgaatgg aacaaattac caaccgttcg attaatttag
     7921 aaaaagtagt aaaagtaaat gatctttcac gtaaatatgt tgcttcagag ctaacattag
     7981 aagaacttta ctgcgaactg caagcaatcg accgagaccg ttctttcttt cctgtctggt
     8041 tgcaaatcgt tagcgcggca gtgatcagtg gaacaattat ggtgttattt ggcggaaaga
     8101 tgaccgattt acctgtgact ttagtcattg gaggattggg atacgcagct tatctatata
     8161 gtttgaaatt ttttagaatc aaatttttat cggaattttt atcttcttta ttgattggca
     8221 tagccgctat ctacagcgta cgttttggct taggaagcaa tcaggatcta atcattatcg
     8281 gatgtgtgat gcctttagtt cctggagttc aaattacgaa cgctatccgt gatttgctag
     8341 ctggacatta tgtctcagga gtttctcgcg gaacagaagc gatgatgact gctacgatga
     8401 tcggttttgc gattgctttt attttccagc ttttttatta ggaaaggaac acaagtatgg
     8461 ggacgatatt tattcagttt tcttttagtt ttttagcgac tgcggcattt gccgttatta
     8521 caaatgtacc taggcgttca ttgatttgtt gtggtctttc tggtgctttt ggctggatga
     8581 tctactggat ctgtgttcaa ataggcggat ctgctgcttt tggttcattg cttggttctt
     8641 taggagtagc attcatcagc gatcttttct ctaaacgttt gaaaatgccc gtaacgattt
     8701 tcaatattcc cgggatggtt cctctagtcc ctggtggttt agcttatcaa gcggttcgaa
     8761 atctcgttac tgaatcctat cgagaagcgg ctcattatgc ggtccaagca attatgattg
     8821 ctggagcaat cgctttaggt cttgtattat cagaagtctt taaccataac atccgtaatt
     8881 tcaaagtaaa gcgtgacgca atcatgatga aacgaaaaaa acggcttttt aaataaacga
     8941 agtgtaacaa agaaaaggtt gtgaaagaga cgttcagcct tgagcgatga caaaatatct
     9001 cacaaacatt gcgacaagta accgcaggag caataagaag taattatgag tattaaaaaa
     9061 agttttgtaa aaatagcttc tcacattttt acataaaatt gacttaatac tgagaaactg
     9121 acttttgaac accctctatc tgtaaaaaag gggctgcgac atgatttttg tcacggcccc
     9181 tcttctttat tcaaattttt tccaaaacgt tcgttgataa taaacgatcc atagagcgga
     9241 aagtattaga actaaaccga tcacaggaat attcactaac caaaatggat aaaacaaagc
     9301 caacattccg tataagacga taagaataat actgatgatc aatgaaccca tcatagttgc
     9361 gactaaaccc aaacttcctg aacctctgtt aaataattgg ttaatattgg tccagttcag
     9421 cagcagcaac cgataatctc tagcgaaata acgtagtgac aataagtagc agccgaataa
     9481 agaaccaaat agcaagctag ggataaaaaa caaaggcagc tgaaaagaca gtccgcttaa
     9541 taatgcgatc ccaccagtta aaaaactctg caatatccat ccgaatctga acttttcttt
     9601 taaatattgg ttcatagaga ttggcaaaga acggataaac agaaaatttt cttgatccaa
     9661 agaaataaga ttgctgataa aagaagtctg attaactgtt aagaaagcca atgcgatccc
     9721 taccaggaaa acaacaccga ttaacttatt atctaaatca gttaatttta tttcgcctcc
     9781 tagggcaaaa gcaagaataa aaatgattgg agttaacaag gaagaagaaa gaacttgcat
     9841 aatcaaatta ggttctttta ctaattgagc attgtaacta aataaaagct gatgcacatt
     9901 ttggttcttt ttatgggttc tttttatttt gaactcactg tttgaatcag tcagttgctc
     9961 tgtaagcttt ggtactatca gctgttttac agcaaggaaa aatcccagag ccgtgatagc
    10021 tagtattccc caacccaata aaccttgttg agaaagtggc tgactggcag catcatagaa
    10081 tggcaataat atcgaaatcg gatgccgatc tgttaactca ttttcaatag tccccgtttg
    10141 atgattcatc cacatgattc cagcaaatgc gatcagcatc gagctgccaa gcaagatact
    10201 agaaactgtt tttctatgtt ttttaaacac tgctgttcga gccaaaccaa aaacaatcaa
    10261 actagccaga caaaaaagaa taatcaaaaa tagagaaaac aaaaggattg agataatgat
    10321 agtaagcaaa atccctagat cagctcgcca tccggttaaa ataaacatga ctagaagcgg
    10381 aaaaacaaaa ggaatgatgg tcaaagcaac taccaaaatc tttgctgaaa aaacctgttc
    10441 ttgtccaaaa ggcagaggca aaaatacttg caggtcattt ccttcaaaaa agatattata
    10501 aatgactgat atactttgag ttaaacctaa cataccaaaa agtgccacgt aataagtaaa
    10561 gaaacctggc aatttaacaa agttcatagc aaacatagca aatccgtaaa tcagtaaaaa
    10621 aatcagccct gaaaaaagat tttgagacaa aagatagata gttaatcgct ttcccttttt
    10681 tcctttctct cttgctttat tcgtgagctg tggattaaca tataaaagat taactcgaat
    10741 taattctaat aattgtcgct tatacatacg gtccatctcc tgtcgcttgc cttccagcca
    10801 ttttcaaata aatcttttct agcgtttcat caggtgagcc attcaataat tcactaactg
    10861 ccccctgata aatcaattca cctttcttca aaatagccag tttatcgcat aactgttgtg
    10921 cggtatccaa tacatgtgta gaaaaaatga ctgtttttcc ttttgcggca tgagccttca
    10981 tcatttcttt caaatcaaat gctgcttgag gatcaagtcc ttgcaaaggt tcatccaata
    11041 cccaaatatc tggatctggc agcaacgtgc cgatcaaaat cgttttttga cgcattccat
    11101 gtgaaaaact tgcgattgtt tcattttgat gactatacat atcaaaaagc gctgtcaatt
    11161 ctgctaaacg cttttctttt ttttgtgtgt ctaattcata ggcagctgaa atcaagtccc
    11221 aatattctcc agccgtcaat tgtaaaaaaa tatctgggga atcaggcaca taaccgattt
    11281 tttgtttgat actcaatcga ttttccgtca atttcattcc atctactaaa atcgttcccg
    11341 atgatggttc aataatacta actaatcttt taatcgtagt tgattttccg gcaccattat
    11401 gtcctaaaaa tccaaaaatt tccccttgtc ttatcgagag agacaagtct tttaatgctt
    11461 gttttttccc gtaactttta cttacattga tgaattcaat catttctgta ctccttttgt
    11521 ttgttattac tttttattgt acccttcgtt attggtggag taaatgtcaa aaagagtgca
    11581 taacaaaaaa ggatagaaac gactaggctg ctacttgggt tgttctcttc tacaggaaaa
    11641 cactcttcta acaggtaaga tatgagtctt tgttttcttt aaaatgattc attttctatc
    11701 ttttcttgca taaaattctt tagtttatca atacttctct ttcccatctc tagttgttct
    11761 ccatttgcaa atgttactat ttcgtttact ctgctaatag atgtaatgtt t
//
LOCUS       DAGCYL010000079        11692 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.080, whole genome shotgun sequence.
ACCESSION   DAGCYL010000079 DAGCYL010000000
VERSION     DAGCYL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.080"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            109..312
                     /locus_tag="MB216_001606"
     CDS             109..312
                     /locus_tag="MB216_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBT4526445.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(413..1075)
                     /locus_tag="MB216_001607"
     CDS             complement(413..1075)
                     /locus_tag="MB216_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4526446.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(1088..2590)
                     /locus_tag="MB216_001608"
     CDS             complement(1088..2590)
                     /locus_tag="MB216_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4526447.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            3138..4817
                     /locus_tag="MB216_001609"
     CDS             3138..4817
                     /locus_tag="MB216_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBT4526448.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4944..5423
                     /locus_tag="MB216_001610"
     CDS             4944..5423
                     /locus_tag="MB216_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBT4526449.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5642..6322
                     /locus_tag="MB216_001611"
     CDS             5642..6322
                     /locus_tag="MB216_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBT4526450.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            6330..7661
                     /locus_tag="MB216_001612"
     CDS             6330..7661
                     /locus_tag="MB216_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBT4526451.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7676..8638
                     /locus_tag="MB216_001613"
     CDS             7676..8638
                     /locus_tag="MB216_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBT4526452.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGL
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8749..9087
                     /locus_tag="MB216_001614"
     CDS             8749..9087
                     /locus_tag="MB216_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBT4526453.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            9166..9873
                     /locus_tag="MB216_001615"
     CDS             9166..9873
                     /locus_tag="MB216_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBT4526454.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(9976..11457)
                     /locus_tag="MB216_001616"
     CDS             complement(9976..11457)
                     /locus_tag="MB216_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4526455.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTTKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHKLYMKKGYENKTKRM"
BASE COUNT         3738 a         2015 c         2436 g         3503 t
ORIGIN      
        1 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
       61 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
      121 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
      181 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
      241 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
      301 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat
      361 atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt
      421 cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt
      481 caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa
      541 gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga
      601 ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg
      661 aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat
      721 ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga
      781 cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat
      841 catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct
      901 ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac
      961 cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat
     1021 aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat
     1081 tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta
     1141 aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag
     1201 gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg
     1261 ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac
     1321 cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc
     1381 ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac
     1441 aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata
     1501 gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta
     1561 tgataatacc ggctacagcc actagtaaaa caacattttt agaaaaactt gccacgttgt
     1621 ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg
     1681 tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag
     1741 tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag
     1801 tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc
     1861 cgcttgtttc gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat
     1921 cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag
     1981 tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg
     2041 tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga
     2101 tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc
     2161 ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc
     2221 catcttttgc catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt
     2281 cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg
     2341 gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt
     2401 ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt
     2461 cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca
     2521 cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca
     2581 taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta
     2641 aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa
     2701 aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac
     2761 gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac
     2821 gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa
     2881 tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat
     2941 tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc
     3001 tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat
     3061 tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat
     3121 aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa
     3181 atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc
     3241 taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt
     3301 tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga
     3361 tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga
     3421 cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt
     3481 tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc
     3541 gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta
     3601 tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt
     3661 atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa
     3721 atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata
     3781 cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag
     3841 tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag
     3901 atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag
     3961 atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag
     4021 aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc
     4081 gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga
     4141 tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt
     4201 tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga
     4261 tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg
     4321 ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac
     4381 gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag
     4441 aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc
     4501 gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa
     4561 agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc
     4621 gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa
     4681 gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact
     4741 tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac
     4801 gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg
     4861 ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac
     4921 gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag
     4981 aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag
     5041 aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag
     5101 gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa
     5161 aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa
     5221 tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg
     5281 attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg
     5341 gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta
     5401 tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc agagacctca
     5461 aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt
     5521 tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga
     5581 tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa
     5641 tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg
     5701 cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt
     5761 aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc
     5821 tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa
     5881 cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg
     5941 cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca
     6001 gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc
     6061 aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt
     6121 cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc
     6181 gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga
     6241 acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt
     6301 ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat
     6361 atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga
     6421 atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt
     6481 caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac
     6541 gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag
     6601 gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac
     6661 gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag
     6721 cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg
     6781 caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag
     6841 tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg
     6901 tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt
     6961 ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc
     7021 atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga
     7081 tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct
     7141 ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact
     7201 tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc
     7261 gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg
     7321 atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac
     7381 aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg
     7441 tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc
     7501 tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg
     7561 aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt
     7621 ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac
     7681 agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca
     7741 tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga
     7801 aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt
     7861 agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga
     7921 tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa
     7981 aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc
     8041 aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat
     8101 gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag ggctcttttt
     8161 gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt
     8221 tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc
     8281 cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc
     8341 gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact
     8401 tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta
     8461 ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca
     8521 tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga
     8581 aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata
     8641 cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc
     8701 tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca
     8761 aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca
     8821 gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg
     8881 gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac
     8941 ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt
     9001 ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg
     9061 aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct
     9121 actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga
     9181 atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga
     9241 attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac
     9301 aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc
     9361 ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg
     9421 agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat
     9481 gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca
     9541 cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta
     9601 ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta
     9661 cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt
     9721 ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt
     9781 gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg
     9841 atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt
     9901 tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga ataagaggat
     9961 gtgacaagaa ttttgtcaca tcctctttgt tttgttctca taaccttttt tcatatacag
    10021 tttatgaacc gcttttatta acttgccaaa taacactgta ttgtgcgttg tatccagata
    10081 gaaagtctcc gtttcatctg aaattttctc atgctgatct gtaatgatca aatcagcatg
    10141 ttctgaatat gcggtaattt taacagcatc tttgcgaaat aactgctgca aatggctttg
    10201 aagatgataa tttcctgtaa actgtttggt tatctgcaaa tggatgagta actgctcttt
    10261 cgtttctgtt tgtagtaagt gttggatcaa ttgactaagg tattgactga tttggtttct
    10321 ttccccattt tttgaaaaaa ttgtatcgat tttttctctt aatttctttt cctgtataaa
    10381 agaactagaa tgattggacg tggcttctgg aaagcaaagt gtagaaaaac tactgctgga
    10441 ttcacccaac agttcgaaaa aaacaactgc caacacaaga aaataaaaat aaattggtat
    10501 tttattttct aaatggttga gaggaaactg ttgagttaag taagtcatca attgaccgac
    10561 ttgtcgacaa ataaggtttt tgcttgtgga aaataaaaaa ccaatctcct gtttttgtcg
    10621 aaaagagatt tgatttggaa aaaaacaata gaaaaaataa ttaaagtagg ctctctctgt
    10681 ttctgtctct tcacaagggc gactcactga aaaagaagta aagtattctg aataatcatg
    10741 tgtatgctgc aataagtcca tcaattcagt gatttcagaa gaagtttttg gaaaatatgc
    10801 ctgttgtttg tttcgtaata tgaataaagt taacagcaag tagatatttt tttcttgttt
    10861 tgaaattttt tctatttgct catctgtact gttcattttt agtgcattta attcaagtga
    10921 ggaaaatggc caatcgttac ctccaaatgt atctgaaaga aaaaagtaaa gatagagacg
    10981 gatagataac tcatttcctt caagagtaca gcaatttttt acagatagga tccgtaaatg
    11041 gaattttttt aaggaatgat tgagctgttt tgtcagtttt tcgagatagg agggcgatat
    11101 ataaagttgt tcaagtaaat atttttttgg tacagcagtt tgtggataat tgattagtaa
    11161 catcaaatag ttaaaacgat tggattcttt taggtagcat aattttaact tagaaaaaac
    11221 agctaacttt ttagtttgat cggtttcttt ggtttggatc tctcctttat gattagaata
    11281 aataaattca tcaatcaata aaaattgctt gagtgataca tttatttttc ggatcagttc
    11341 tttggttgtc cgttttggaa tatgtaagtc attggataat tcttgtaaag aacaattatt
    11401 tttttgaatg accatttgta gaacaagtaa ctcttttttt cttgcttcaa ttagcataaa
    11461 tacctccttt ttattctgtt tttgtatata gttagacaaa aactgtgagt ggaaagtcat
    11521 ttcagtctgt gttattctca cagagtgtcg ccttcttttt tatataacgt tatttaattt
    11581 ttttgttacc ttgtttattc tattgtttat ttcctagggt attttgtcaa gatattcttg
    11641 tttttaaggc ggaaaaattg atttttaatg gatataacta aatatgtttt ga
//
LOCUS       DAGCYL010000080        11563 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.081, whole genome shotgun sequence.
ACCESSION   DAGCYL010000080 DAGCYL010000000
VERSION     DAGCYL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.081"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            70..3297
                     /gene="fss3"
                     /locus_tag="MB216_001617"
     CDS             70..3297
                     /gene="fss3"
                     /locus_tag="MB216_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBT4526456.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            3409..3723
                     /locus_tag="MB216_001618"
     CDS             3409..3723
                     /locus_tag="MB216_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4526457.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3736..4110
                     /locus_tag="MB216_001619"
     CDS             3736..4110
                     /locus_tag="MB216_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4526458.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            4111..4464
                     /locus_tag="MB216_001620"
     CDS             4111..4464
                     /locus_tag="MB216_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBT4526459.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTGEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            4533..5549
                     /locus_tag="MB216_001621"
     CDS             4533..5549
                     /locus_tag="MB216_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBT4526460.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            6143..8017
                     /gene="ltrA"
                     /locus_tag="MB216_001622"
     CDS             6143..8017
                     /gene="ltrA"
                     /locus_tag="MB216_001622"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBT4526461.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            8143..8499
                     /locus_tag="MB216_001623"
     CDS             8143..8499
                     /locus_tag="MB216_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBT4526462.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            8544..9686
                     /gene="dcm"
                     /locus_tag="MB216_001624"
     CDS             8544..9686
                     /gene="dcm"
                     /locus_tag="MB216_001624"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HBT4526463.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            9676..9965
                     /locus_tag="MB216_001625"
                     /pseudo
     CDS             9676..9965
                     /locus_tag="MB216_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10221..11405
                     /locus_tag="MB216_001626"
     CDS             10221..11405
                     /locus_tag="MB216_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBT4526464.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            11402..11515
                     /locus_tag="MB216_001627"
     CDS             11402..11515
                     /locus_tag="MB216_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBT4526465.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         3875 a         1983 c         2576 g         3129 t
ORIGIN      
        1 actttataaa caaagatgtt caagcgagca tcttattttt ttgtgcctaa tttgaaagga
       61 gaacatttca tgtataaaaa acaaaaaaag aaattatgtg tattaactat tttgagtttg
      121 gctgttcaat caattgtagg agcaattgct cctacgattg cttttgctga tgaaattacc
      181 catccacaaa cggtaaccgt agaactagat ttagcacatc aatatgctgt tgaaggaact
      241 tttagtgatg gtcgtccaat gtcagaagtc actgttccgc attatgcagt gtacaacggc
      301 gttaaacaag atattttctg tgttgaacca ggagtcccga tttacaatga gttcactcct
      361 ggatatgaga agaacccgtt accagatatg tccgataaag ccaaattagt ttccgtttta
      421 tggaaaaaag taggtacaga tgcagatact catattgtgg cacagaagat gatctggcaa
      481 gaagttaatg ggtataccct tcactcaata aaacgatcag atggtagtgc cgtaaatatc
      541 gcagccattg aggcaaaaat caatcaagcc attgccgatt atcagaagaa accaagcttc
      601 cataatagta cggctaaaac agtattaggg caatcgacta ctgtgacgga tacgaataat
      661 ctgaatttgt cagagttcga tgaggttgtg gaaaataccg caaacattga ttatcgtgtg
      721 aatggcaatc agttgataat cagcccaaat gccaattcaa aagaaagtgg tgtgttgacg
      781 cttaagaaat ctgctggtac cggaacacct gtcgcctata aaaaggctgg ccaacagact
      841 ttaatggccg gggcgatcga taagccgaac acttatacgg tcaaaattga tgtggaaatg
      901 gagggttctt tgaagattaa aaaagttgat aaagaatcgg gtgatattat accaggaacg
      961 gttttccatt tagactttgg aaaaacttta cccgcaaaag acgtgaccac cgataaagaa
     1021 ggcattgcga ccttagatga gattcctcat ggtacaaagg taaccattac tgaaaaatcg
     1081 gtgccggcgc cttatacgat tgataccaca cccatgactg ccacaattaa agcaggtgaa
     1141 accatttcag tgacttcaaa aaatacgcga gaaaaaggtc aaattattct ggataaaacc
     1201 ggaattgaaa cagggagtga tctttggaac gataattact ctttagcggg caatacgttt
     1261 gccattcgca aggatagccc aactggtgaa atcgtccaag aaatgaccac ggatgaaaaa
     1321 ggtcatgcag aaacaccaaa agagattgcc aatgcgttgg aattgggaac ctactatgtg
     1381 actgaaacca aggctagtca tggctttgtg aatactttca aaccagtaaa agtcgaattg
     1441 aattatgtca atcaaacagt ggctcttgtt accagtaacg taaaagggca aaaccaagaa
     1501 attactggtg aaaccacttt gacaaaagaa gacaaagata ccggtcatga aactcaaggg
     1561 aaagctgagt ttaaaagagc tgaatacacc ttgtttactg ttaaggatgg aaaagcggtc
     1621 aaatggaccg agaactttaa accagaattg ttgaagggaa cgaaagcttc tgatgaaacc
     1681 gtaactttga ctttagatga aaagaatcaa gttgccgtta aacacttagc gattaatgag
     1741 tattactggc aagaaaccaa agcgcctgaa ggatatacct tggatgaaac aaagtatcct
     1801 gtatccatca aaaaggtgga tgatagcgaa aaaaatgctg tgattactcg agatgttgcg
     1861 gcaaaagaac aagtgattcg ctttggcttt gatttcttta aatttgctgg atcaactact
     1921 ggcactgccg aaactggctt caatgatcta acctttaaag tgtcgccatt ggaaggaacc
     1981 aatgaaatca ccggtgccga agatgaagcg accacagctt ataacgagca attagggttt
     2041 gatggttatg gcaagtttga aaatcttcct tatggggatt atttacttga agaagtagaa
     2101 gccccagaag gttttcaaaa gattacgcca ttagaaatcc gttctacttt taaagaaaat
     2161 aaagaggatt ataccaagag tgattatatc tttactatta ccgaaaaagg gcaaaagcaa
     2221 ccgattaaaa cggtaacggt cccttatggg aatctaacca atgagaattt ctcagtcagt
     2281 ttgaaccgtt tgatgcttta tgatttgcct gaggaagaag atagcttaac ttctcttgcg
     2341 acttggaaag aaggagataa agaattgact actcttgatt ctaccgagct agtcgataaa
     2401 ttgagctata atctacatga aatcaaagaa gactggtata tcgtagctca agccatcgat
     2461 gtagatgcta caaaagctgc ccaagaaaaa gataaaaaag ccaaaccggt ggcgattgcg
     2521 gaaacaaccg caactttagc gaataaagag aaaacaggaa cttgggagat tctgcataaa
     2581 ttaactgtcg aacaagtttt agacaagacc attgtcttat tcaactatgt gtatgaaagc
     2641 aaagaagcct atgaagctgg tgatgagccg gtagcgaagg atgctagctt gaacaatcaa
     2701 gcgcaaactg ttaattgtat ggtggaacgt catgtttcca tccaaaccaa agcgcactta
     2761 gaagaaggtt ctcagacctt tactcatggt gacgtggtag atatgtttga tgatgtatcc
     2821 attacccatg atgtactaga tggctcaaaa gaagcttttg aaacaattct gtacgcttta
     2881 tttccagatg gtacaagtaa agaaatttgg aaatctggca agattgatta cgaagtgaat
     2941 gacaaagaat ttaccaaaac cgtacttgcg gaaaaagtgg atacaagtaa atatccagaa
     3001 ggtacaagtt ttaccttcaa agaaattaac tatgacaaag atgggaatat caatggaaaa
     3061 cacaatgaag atttgaaaga aaaatctcaa actttaacgc ccaaaaaagt tccgacaaca
     3121 ccaagtacac cagaacaatc tgaaatgcca actgttccaa gtggctctca agaatctagt
     3181 cctacagtga aggcattccc tcaaaccggg gagaaaactt ccaatgtgct actgttcatt
     3241 ggctttagct tgatctttgc gacggctggc tattattttt ggaatcgtcg gaactaaggt
     3301 gataagatga aacatgtaaa tagtagaaaa caatacgcgc gaccgcctcc taaaaaagtg
     3361 gtcgcgctat ttttgtattc aaaactaaca atgaaaagag gaaaaaatat ggaattgaaa
     3421 tttgttgtac cagatatggt cgagacgttc ggaaaactaa catacggtgg tgaaggtgaa
     3481 gttctatcgg aagggtatgg tcgaaatact acaattgttg gtcgaagtta ccatctgttt
     3541 tcaaataaac aacgggccga tgatattgaa gtggtggtag ctgcggaagc cggcgaaaag
     3601 gattttgacc aagatcaacc actaaaagct gtaaatcccc acttaattgc caaaggctat
     3661 gaaattgaga atcgtgggtt taccgattac gtgttgtatg ttgatgattt agtgaaagca
     3721 taggaggaaa agaagatgag attaacagaa ggtattgtcg tagattcagg attaaccttt
     3781 ggcaagttgc gtttttccgc attacgtcga gaagtacgca agcaaaatga ggacggaacg
     3841 gttagtaacg aggtaaaaga acggacctat aatttgaaat cctccgcgca aggtcgaatg
     3901 atccaagtca gtattccagc aaatgtaccc ttacgcgagt ttgcttatga tgcagaggta
     3961 gagctggtca atccaatcgt ggatacggtc gccaattatg tttttcggga aggaaccacc
     4021 gttaattggt ttatcaaggc agatgattta gtcttgaaac gacaactgaa tcaaggaaaa
     4081 cctacgaacc aaaatgaagg aaagaaatag gtgaagaata tggaaattaa tgttgaacat
     4141 attttagatt gtctcgatca atttggcaaa ggtgagctaa cgggggaaca actcactaac
     4201 gcattaacct atgatgaaaa aatgtttctg attatgcatc aagggttact ggaagtaggt
     4261 aacgatgcca aagaagactt tgatgtactg gattggttag gggaagaaga gtctttcttt
     4321 atggttgtcg aggtaaatga aagtttatgt cggcaggcag aatctgtttt ggaagaaatc
     4381 gatgtggaga tgccggatgc catcgaaaga tttttaaaac aattagtgga aacgaaacaa
     4441 cttcccgtag tagtaaatga ctagcataac tttttgaaat tgcccaatga aatcctattg
     4501 ggctttttcg actggaggtg gaaaccatca aaatgtacaa aggacatcgc atacgcgcag
     4561 gagatcaaca cttgatttat cactttgttc ttggctggct aatctttttg ttcattggtt
     4621 ggatgagcgt cttttattta caagaactta gacaatttga tatttctaag gtatcatttt
     4681 ccactagtga aattggctgg tctatgaagg atgtaatctg cttgctagga agtctcgctc
     4741 tttcaggaag catgatgtta ctttatatac acttttttca ggatcattgg cgaagtcttt
     4801 ggcatcgaca aaagttagct cggatgattc tagaaaacca ttggtatgaa gtaaagcaga
     4861 ctcaaggtga aggttttttc aaagatttga atagtagttg gaccaaagag agtatcagtt
     4921 acttccccaa aatttattat cgaatgaaag atggcttact ttctattcgc gttcaaattt
     4981 cactgggaaa atatcaggat cagctattga agttggaaaa taagttagaa agcggactgt
     5041 attgtgagtt agtagaaaaa gaactcaaag actcctatgt ggaatacacc ttgttatacg
     5101 atatgattgc caatcgaatt gggatagaag aaatagtggc agaaaatggg gcgttgcgat
     5161 tgatgaaaaa tcaagtatgg gcgtatgatt ccttgccgca tatgttaatt gctggcggca
     5221 caggtggtgg gaagacctat ttcttactca ccattattca agcactattg aagtcagatg
     5281 cagaattgtt tatcctcgat cccaaaaatg cggacctcgc cgatttaggt acgataatgc
     5341 ctcacgtata ctcccaaaaa gaagaaattt ctgaatgtgt ggaagatttt tacgaacgca
     5401 tgatagctcg tagtaggtca atgaaagaaa tgcccaacta caaaacagga gagaattatg
     5461 cgtatctcgg acttccgccc aactttttaa tctttgatga atacgtggct tatatgggtg
     5521 cgaatcgttt tcctacaagc atcgaataga tgtgaaatgg aagcctattg agattatcaa
     5581 tagtgacaac taatgttttg agagatggaa tgaaagcctt caagttgagc tgaaacatcc
     5641 ccgctaatac tcctacatgc tctaatcaat gaagtagatt ggttttggta tgaagctagg
     5701 tgaagtcgtc tgaaatctgc ctaagtgctt gagcataagg ctgactatag ggcgggcggc
     5761 tgaaaaagat aactatggta agaatgtctg aaaatgactg acgaatctgc gaatgtacgg
     5821 gtctaaaagg ctgatcttat tgaaagatag gaacatacgg acgtatggtg agataccgac
     5881 aagtaaggtt ccatgatatg aaaatcggga tagctaacga agaagctata atttcacagg
     5941 ctcatagtag atacctaagg ttatacatga tagctaaaaa cattggaacg atgtaagcag
     6001 aaaactatca attaaatgac tacttctaga taggtgcata taagtaataa cgaaattgct
     6061 ttgtttctgt gaaagtaagg gcacagtacc gatgaagcgt ctaacaaatg tggagggata
     6121 gccctaagtc tatttaataa caatgaatgt tgaaacagat gaagttcaag gatctggtaa
     6181 gaacgtggga aaatccgtta tggaggtgcc acgcttgaac acaaaaatac gttactggga
     6241 atattatgat ctacaagaaa cattcgatca gctatttact caaagtagaa acgggaaaaa
     6301 gttttatcaa ttatatgagt taatcatttc tgaaaataat attcttttag cataccgtac
     6361 tatcaaggca aacaaggggt cttcgacacc aggaacggat tccttcacaa ttgataacta
     6421 taaggaaatg aaccaagcag agtttatcca tcttattctc agtcatttag aaaattacaa
     6481 accaaaatcc ataaagcgag taatgatacc taaacccaat ggtgagaaac gcccattagg
     6541 aattccatgc atgattgatc gaatgatcca acagatgttc aagcagatac tggagccaat
     6601 atgtgaagct aagttttatg aacatagcta cggtttcaga ccattgagaa gtgcaaagca
     6661 tgctctaggt cgcattatgt atctcataaa catcagtaaa atgcactatg cagtagacat
     6721 tgatatcaaa ggtttctttg ataatgtaaa ccaccgttta ctgataaaac aattatggaa
     6781 tataggaatt tgtgacaaac aagtcttagc tattctttca aaatctctga aatcacccat
     6841 tcaaggagaa ggtatcccga gcaaaggaac gatccaagga ggtatcattt ctccactatt
     6901 atctaatgtt gttctaaacg acttagacca ttgggtctca aagcagtggc atacctttga
     6961 aacaaagtat ccctatacta aaggttacaa taaatttcgt gctctaagag atacaaatct
     7021 aaagcaaggc tatattgtca ggtacgcaga tgactttaaa atcatgacta atgattatcc
     7081 gacagcatta aaatggttcc acgctgtcaa gctttaccta aaagacagac ttaaattaga
     7141 tatttccaat gagaagtcta aaatcgtcaa tttgcgtaaa tgtaagtcgg aatttttagg
     7201 attcgctatt tgcgtgaaac aaaaagggaa aaaatgggtt tgtaattcac gtatttctaa
     7261 caaaaaaaag gatcagatta aagaagagat taaacaaaga atcaaagata ttcaaaagag
     7321 tcctacagca caaaatgctc tcctgtttaa tgctctcgtg ctcggtgttc ataattattt
     7381 caggtatgcc actcatatta atttagattt gaataggatg aactacgttc tttcattcac
     7441 tacttacaac cgattatcaa attgtagtac aagatgttac cccaacaaag cttccccaac
     7501 ttacaaaaag ttttactcaa caacgatgaa aacgtataaa gtagctggag tctatttgta
     7561 ccctctttgc gacgtatcta caaaaacaat ttacggattt aatacggagg atacaccgtt
     7621 tactccgttt ggtcgtcaaa gattggagca caaaagccta cagtctcttg tgttccaaga
     7681 attaagaaaa ttgatggaat cgaaaatacc gaatagaacg gttgagtatt tagatagtag
     7741 aatatctcgt tactcaatga aatcaggcaa gtgtgagatt acaaaacaat ttttgccagc
     7801 aaaagccgtt cactgtcatc actatttgcc aaaaagtctt ggtggagacg acaaattcga
     7861 taacttgaga attattcata aggatatcca tcttctgatc catacaacaa ataaaatgat
     7921 aatcgatcac tacgtgcatg aactgaaact ggtgccagag caaatagcaa aaatcaacct
     7981 ttatcgaaag atgtgtaatc tgcaaaacat tcaatagaaa ataaatagat ggaacgccgt
     8041 atgaggtgaa agtctcacgt acggtgtgga gcgggggaaa agatggagat tatatcaaat
     8101 tcttacctat cgctatagat gttaacgaca aaagaaagtg cggtgatttt gaataagctt
     8161 aaacaaatcg tgatgttagg ccgtcaatct ggtttctttc tgattctagc ttgtcaaagg
     8221 ccggatgcga aatatctagg agatgggatt cgcgatcagt ttaacttccg tgtggctttg
     8281 ggtcgtatga gtgagcttgg gtattccatg atgtttggag aagtcgataa aaattttttt
     8341 atgaaacgca tcaaaggacg aggttatgta gatacagggg gcagtgtgat tagtgagttt
     8401 tatacgccac ttgtaccaaa agggtatgat ttcttaaggg aaattagcaa cattgttgga
     8461 ttaaccgttc atacggaaag agaaaataat agtgtgtagc ctaggaatat caataatcct
     8521 gggctttttt attgaggtga tttatgacat ttttagattt attcgccggg attggtggct
     8581 ttcgcttagg tatggaacaa gccggtcacc agtgtatcgg tttttgtgaa attgatgaat
     8641 ttgctcggag gagttataaa gcaatccatg atacaaggaa ggaggtggaa atgcatgaca
     8701 tcacaagtgt atcagacgaa tttgttcgat ccctcggacc agtggacatc ctttgcggcg
     8761 gctttccgtg ccaagctttt tcaattgcgg gaaagcggca aggattttcc gatactcgag
     8821 gtactttatt ctttgaaatc gctcggttcg ccactcttct ccaacctaag tttctattcc
     8881 ttgagaacgt ccggggattg ctcaatcacg aaggaggggc tacgttcgag acgatcctcc
     8941 gaacgttgga tggattgggg tatgatgtgg aatggcaagt gcttaactca aaggcctacg
     9001 ttccccaaag ccgtgagcgg atcttcctta tcggacattc tcgagatgca tgtaccgaac
     9061 aagtatttcc tatcattgga tcgtcttcaa catctgatca aaacatcaga aacttggtaa
     9121 atatcaatcc ttccaatcga ggaatgggtg gccaagtcta tggttcagat agcgtagcac
     9181 caaccttgac cggtgatgaa ggaattaaaa ttgccttacc ggtaaaggat gggatttctg
     9241 tagcgggtat gttgtcggga aattttgaac aaggcaatag gatttatgag gtaaccggaa
     9301 cagcccccac tttgtcaacg aaacaagggg gaacgaagat tatgattcgg aaaaatgaaa
     9361 cagaatatca agaggtgaag ccaggggaca gtgtgaacct ggcttttcct aattccacaa
     9421 gtcgccgagg tcgtctggga aaacaaagtg tgcatacctt actaacagga gaccaacaag
     9481 cggtagtcac ggatcagtac cagattcgaa agttaacacc tagggagtgc tggcggcttc
     9541 agggctttcc agattgggct tttgatcgag cttctcaagt caattcagat agtcagttgt
     9601 ataaacaggc cggaaactca gtaacagttc cggtcatttt tgatattgct agaagattaa
     9661 agggggtaaa agcatatgat ttatgacgag ttgattggag aaatctactg ggtgattggc
     9721 aagattcagg cagatccgaa attggaagaa gaactgtacc ggctcaattt tgatattcgc
     9781 aaaaacgggg tgaaagtcca ggtgattcgt atgtagtgga tgaggaaacg gatgctcgaa
     9841 tggagctcaa tcaagtcatc ggtgaattgg aacgaattgc ggaccttgcc aaagaacctg
     9901 atatccgcca atacttgttt gagataaaag cagagttgga aattgagggt atcaccgcgg
     9961 agtaataggg aaataaccca ctgataggca ctgaccgcgg gggatttgcg aagcagaaat
    10021 cctccgtggt gctgtgcctg ttcatggacg gcggcagccg gccatgatgt ctcacccccc
    10081 gtatctaaca ggggggtaca aaaacacaaa aaacaagcaa gtaaccataa aaaatgcttg
    10141 ttgtatcaag gatattaagt aatttgtata ggtgttaact catttggaaa agttaacacc
    10201 ttgaatttgg aggtgaaaac ttggcgcaac gaaatttaga ttatcggtta ttaaaagaac
    10261 gacgcaacga atatggcgtt tcacaaaata aattagctac tgcttgcgga cttagccgcc
    10321 catacctaaa tcagatagaa aacggtggcg taatcgcctc tacaaagacc atgagaaaaa
    10381 tttttgatca actggaaagt ttcaatcccg atttaccttt aacgttacta tttgactatg
    10441 taaggattcg ctttcccaca acggatgtgc ggaaaattat tcaagaaatt ctccatttga
    10501 aattcgagta tatgcttcat gaagattacg ccttttactc ctatcaagaa caatatgtca
    10561 tgggcgatat tgtagtgatg ttatctcatg aagaagataa aggtgtcctt ttggaattaa
    10621 aaggtcgtgg ttgccggcag tttgaaactt ttttactcgc ccaaaagcgt agctggtacg
    10681 acttttttga agattgtcta aaagccggtg gcgtgatgaa acgcttggat ttagcaatca
    10741 atgatcgagt ggggctactg gatattcctg atttaacgaa aaaatgtcag aaggaagaat
    10801 gtatctcctt gtttcgtacc ttcaaaagtt accgttccgg ggaactgttg aaagctgatg
    10861 aaaaggatgg tatgggcaat accttatata ttggcagcct caaaagcgag gtttattttt
    10921 gtttgtacga aaaagattat gaacaatata tcaagttagg gattccattg gaccagaccg
    10981 aaacgaaaaa tcgttttgag attcggttga aaaatgaccg tgcttattat gcgattcaag
    11041 atttgttaaa aggtcgtagc attgaaagta ccacgttttc cattatcaat cggtatttgc
    11101 gatttgcgga taaagtagat ggaaaacggc gaaccaattg gcccttgaat gaacaatggg
    11161 gtcgttttat tggacggaat cggaaagaga ttcaattaac ttctgaaccg aaaccgtata
    11221 ccattgaacg aacattaaat tggctaggta gacaagtcgc ccctacgtgg aaaatggcta
    11281 aggagttgga tcgattgaat caaacaacgt atatccagga tatggtacag aatgcgcgac
    11341 tctcggaccg gcataaaaag attttggagc aacaaagtat ggcaatcgaa aacttgattg
    11401 tatgaaaaga ggaattttga tggaaattgt aaaagatatg ttattggtac ttggtggcag
    11461 tatgcttggt gttaccgtga tgtgcttgat gaatgcaagt gcagcagcgg attgagcaat
    11521 ggaaaagaaa ggaaagtgac aaaatgaatt tcggacaaaa ttt
//
LOCUS       DAGCYL010000081        11470 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.082, whole genome shotgun sequence.
ACCESSION   DAGCYL010000081 DAGCYL010000000
VERSION     DAGCYL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.082"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(35..2110)
                     /locus_tag="MB216_001628"
     CDS             complement(35..2110)
                     /locus_tag="MB216_001628"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBT4526466.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKITNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLNQIAAMALSL
                     ASLDQIITK"
     gene            complement(2112..3029)
                     /gene="glyQ"
                     /locus_tag="MB216_001629"
     CDS             complement(2112..3029)
                     /gene="glyQ"
                     /locus_tag="MB216_001629"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBT4526467.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3413..4225)
                     /gene="recO"
                     /locus_tag="MB216_001630"
     CDS             complement(3413..4225)
                     /gene="recO"
                     /locus_tag="MB216_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBT4526468.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4368..5267)
                     /gene="era"
                     /locus_tag="MB216_001631"
     CDS             complement(4368..5267)
                     /gene="era"
                     /locus_tag="MB216_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBT4526469.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5282..5677)
                     /locus_tag="MB216_001632"
     CDS             complement(5282..5677)
                     /locus_tag="MB216_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBT4526470.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5661..6137)
                     /gene="ybeY"
                     /locus_tag="MB216_001633"
     CDS             complement(5661..6137)
                     /gene="ybeY"
                     /locus_tag="MB216_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBT4526471.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6154..8346)
                     /locus_tag="MB216_001634"
     CDS             complement(6154..8346)
                     /locus_tag="MB216_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBT4526472.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8370..9341)
                     /locus_tag="MB216_001635"
     CDS             complement(8370..9341)
                     /locus_tag="MB216_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBT4526473.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10047..11426
                     /gene="brnQ"
                     /locus_tag="MB216_001636"
     CDS             10047..11426
                     /gene="brnQ"
                     /locus_tag="MB216_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBT4526474.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         3083 a         2419 c         2106 g         3862 t
ORIGIN      
        1 gtgcctgttc tctggtttat ttacatatta gaaattattt tgtgatgatt tgatctaaac
       61 ttgctagtga caaagccatg gcagcaattt gatttaattg cgttaatcgg ttcttacgta
      121 ctgctggttc ttctgccatg accatcgtct gatcaaaata attttcgatc agtgggcgaa
      181 ggcttacaag ctgatcataa ttttcgttga tcgtttggtc agcaaagctt gttttcattg
      241 cctctactgc tttgtgcagt tctttctccg cttcgttttc gaacagttca gggtctactt
      301 ctgattgttt ttcaagctcc gcttttttcg ctaagttcac gacacgtgtc aaagcttcca
      361 tcgatggttt gaagtcttca tctgcaagat gctgcttgaa caattgtgct gctgcaaata
      421 cttttgtcag atctttttgt tctgcgttca aaacagcttc gatgacatca tgacgaatat
      481 tcttcgttgt caataattgg cgcagacggc ctttgataaa gtcgatcacc tgttgttgtc
      541 ccgctgacaa gccaatgcca aaacgatccg tatctttact gataacttca ctaatcgttt
      601 cttgcagaac agacaaagga aaggcccatt ttttcgattc gatgattcga acaacaccat
      661 atgcttgacg gcgcaaagca tatggatcgt ttgagccact tgggatcatc ccaactgcaa
      721 agaaagaaaa gatactatct aatttatcag caatcgctaa aacagaacct actgtagtct
      781 caggtagatc tccttcactg gatataggca tgtaatgttc acggatcgct gttgctacgg
      841 ctggtttctc gcctttaagc agagcatatt tttctcccat gatcccttgt agttcaggga
      901 attctcctac catgttggtt actagatcaa atttatagat ttcagcagcg cgttgcagat
      961 ccatcaattc ttcctcagat agtcctactt gttcgcctat gatgcgagcg atgacttgta
     1021 cccgttgcat tttctcgtag atgctaccga ttttttcatg gaaagtaaca gtttttaatt
     1081 tttcaacgta ttggtcgatt gaagctttct tgtcttcatt ataaaagaat tccgcatctt
     1141 ctaaacgagc agtcaatact ttttgattgc ctcgtaccac attttccaat tttacccgat
     1201 cgccattacg agcagaaata aaatgaggaa gcaacatacc ttgatcgttc cgtacttcga
     1261 aataacgttg atgttctttc atagaagtga ctagtacttc ttcaggaaca gatagatatt
     1321 tttcttcgaa gtcgccagaa aagacagttg gatattcgac tagattgttg acttcttcta
     1381 gtaaatcttc atctagatca atgatccagt gatttttttc tgctaattcg tttgcttgtg
     1441 tgatgatcat ttcttttctt ttttgcggtg aagcaataac aaattgttca tttaattttt
     1501 cttcgtattc ttttgcatgc gtaaatgtca catcttcacc taagaaacga tgacctcttg
     1561 aagctctgtc tgttttgata tccaagatct caaacgggat tacttcgtca tctaacaatg
     1621 ccacgatcca gtgaataggg cggatatatt caaaatcgta ttctgcccaa tgcatcgtta
     1681 ctgggaaagt gaggctttta atgacatctg ctaaacctgt caatacttct tttgcttctt
     1741 tgccgtgagt gtatttcgtt acatagacat attccacgcc ttttaattct ctaaaagtga
     1801 tagcatctgt cgtcagacct tgtccgcgga caaatccttc agctgctttc gtccagtttc
     1861 catctgcgtc caaagcaatt tttttggctg gtcctttgac ttcttcttca ctgtctgctt
     1921 gacgttctgg aatgtttgtt attttgattg ctaagcggcg cggtgtagaa aacgtttcga
     1981 tcgtatcata agtcaattgg ttttcatcta aaaattttgc tgttttttct tctagttgtt
     2041 tcatgcttgg tgtgacaaca tgagcaggca tttcttccag cccgatttca agtaataagt
     2101 cttttgccat cttattcagc ctccttcatg ttttctggat ttgctgtgtt tttattcaat
     2161 aaagggaatc ctaatttttc gcgttctgcc acaaagattt tggctacagc acgagccatg
     2221 ttgcggatac gggaaagata acctgcacgc tctgttacgg atactgcacc gcgagcatct
     2281 aataagttga atgtatggct gcatttcaaa atataatcat acgctggatg aacaagattt
     2341 tcatcgatac agcgtttagc ttctttttca aaacggtcaa aattgcttag tagcaagtct
     2401 tggtcgctta cttcaaaaga atatttggaa tgttcatatt ctggttgttt gaagatctcg
     2461 ccatatttca caccatcagc ccattccaga tcatagacac tttcaacttc ttgaatatat
     2521 gaagcaagac gttctagtcc ataagtaatc tcagaagtaa ctggtttaca aggtagtcct
     2581 ccgacttgct ggaagtacgt gaattgtgtg atctccatcc catcaagcca tacttcccag
     2641 cccacaccgg cacaacccat cgatgggttc tcccagttgt cttcaacaaa acgaatatcg
     2701 tgttctaatg gatcaattcc taaaagacgc aagctttcta aataaagctc ttggatattt
     2761 tcaggtgaag gtttcattac gacttgaaat tgatgatgct gatacaaacg gtttgggttt
     2821 tcgccataac gcccgtcagc tggtcggcgg gatggctcta cataagcagc attccatggt
     2881 tccggtccga ttgcccgtaa gaatgtatat gggctcatcg tacctgctcc tttttctgta
     2941 tcataagctt gcatcagcat gcagccgttt gatgaccaaa atttttgtaa tgttaaaatc
     3001 atatcttgga cggtcaattt tttactcatc tgttttgccc ctttcaattt ttaagcgttg
     3061 ctgtcaataa gaatacagga aagaaaacga caaaaagtcc ctatgtctga ttacagacat
     3121 agggacgaat aatttcgcgg ttccacccta cttccgaata aagattcggc agcttcattt
     3181 atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc ggcttccact ctcccgaact
     3241 cgctaaacca cgtgtttttt actgatcttg gtcaatgtac gtattcattt tgcagttttc
     3301 ttgtttatat aatagatcct ctcagtgaac gatctattta atctacggtt aataatagac
     3361 ggaagtccct gtatttgtca aatagttttg atttttttgt tgtatctttc atttattctt
     3421 ctttttcagg tatctctttt tcaggcatat ttttctctgg cgtctttaat acgtgtgccc
     3481 aagacttcat ttgatcaatg aactttttgc tttttagatg aataccgaca tattcatcat
     3541 acaactggtc aatgaggtca cggattgcct gcttcgtttc ttctttcaag tggattccac
     3601 tgatcttatc ataagaaata gccgaaaaca ttcgaacgaa ataaacagcg cgaggatctg
     3661 catgataacg gtgttcgtcc attggccagt gtttctcgca tatcacaccg ccatatttgg
     3721 aagaatagtc aaattttcct tttgtttccc cgcatacgac acagtgcgtc caaattggcg
     3781 cgattccgaa gcgttgcagt atttgaactt caaagatatt cgtgatgatt tctgcatcat
     3841 ctccttgatc catccgctgc agagcttgtt gcgtaaattg gaataaatgc gggtcatatt
     3901 gatgatcttc gattgctgca tctactagat ttagcaaata ggtaccataa ccgctcaaaa
     3961 agatatcttc ttggatgttc cgaaacgcat ggacgtcttt gacactgctt aaaaacgaca
     4021 aaccttcact tcgaaaatta ccgatataga ctgcttcggt aaaggggagc aaagccggag
     4081 ctaatggctg attttgccga tggattcctt tgatataaaa catcattttg ccagaagatt
     4141 ccgtaaagat tttgactaat ttatcttttt ctttgtaatt acggctagat aaaatcaagc
     4201 ctttcgattc tgcaagctgg ctcatattct ccctcctaaa aaataaatct ggaaaagcag
     4261 attctgccga tccagattat ttatgtttcg atcttccgcc gtttttctca acgtgcggat
     4321 ttttatccag tttatttcta tacttaacct ataagaagtt cagaatatca gtattcgtct
     4381 tttcggtagc caaagtcttg cagatagact tttttgtctc gccagtcttt ttggactttg
     4441 acccaaagtt ctagaaatac tttgtctcct aaaagatttt cgatatcctt tcttgctttc
     4501 gttcctactt gcttcagcat ttttccgcct ttgccgataa tgattccttt ttggctatct
     4561 ctttcgacga tgatcgtggc ctggatatgg attttatcat tctcgtttcg cttcatcgaa
     4621 tcaacaacga ctgcgacaga atgaggcact tcgtctcgtg ttaaaaacaa gactttctca
     4681 cggatcaatt cagaaacgat aaagtattca ggatgatcag tcacttgatc atctggaaaa
     4741 tattgtgggc cctctggcat ttcactgact aatacatcca ttaagcgttc gacattgttt
     4801 ccttctgtag cagagatcgg aatcacttgt gcaaaaggca tctgcgtcga ataatcttcg
     4861 atgattccca gcaactcgtc tggatggatc ttatcgattt tattgataat cagataaact
     4921 ggagaatgca cgttttttag acgttcaata ataaaatcgt ctccccggcc gcgtttttga
     4981 tcagcactga tcataaataa gactgcatcc acttctttca acgcactgta agctgcttct
     5041 accataaaat ctcctagacg atgctttggt ttatggattc ctggtgtatc gataaaaatc
     5101 agctgagctt ctggtgtagt atatactcct tggattttat ttctagttgt ctgtgccttg
     5161 tcactcataa tggcaatttt ttgacccacg attcggttca ataatgtgga ttttcctaca
     5221 ttcgggcggc cgacgattgc gacaaaccct gatttatgtt ctggcatata cgttctcctt
     5281 tttaattaaa caacaaatga tatattttag ggacaaataa aatcaaccca atgatgacag
     5341 caaaacaagc ggtcactaaa actgctccag ctgccatatc tttgattttt ttcccaatcg
     5401 gatgaaaatg aaaatccgta aacatgtcca ctacattttc aaaaacagta ttgatgatct
     5461 ctactatcca aacgagaaaa acagctaaaa gcagccataa ccactctatc cgatcaagct
     5521 ggaaaatcac gcccataaat aaagcaagga taccgaacgc aacatgtttc ctcatattgc
     5581 gttcttcctc aaaaacggtt ttgactcctt ggacagcaaa ttccacagac atcaggaaat
     5641 gtttgttttt ctctacgttt ttatcttttg agtccatagg catccaagat ctctttctgt
     5701 aaaccaaaca tttctttctc atcttctggt gtcatatggt catacccgtt gatatgcaag
     5761 aatccatgta ccgctaaaaa gccaagttcc cgatcatatg aatggccgta agcttctgct
     5821 tgttcagcag cacgttcaat agaaatcatg atgtctccta gtacacgagg caatggattc
     5881 tctccatctt caaaaatcac tgggatttcg tcttcgcctt cttcttccag agcaaaactg
     5941 atgacgtctg tcggtgcatc ttttccacgg taatcacgat tgattacttg gattgccgca
     6001 ttgtccatga aagtaacaga catttctgtt tcctctggca gctttaaata agatgctgca
     6061 aattgcagca agtcatcgat ttctttcatt ttctcttctg agacttggtt tgtttcatcc
     6121 ataaaggtaa tatccattat acatcgcttc tttctattct tcttgttctt cctttatctt
     6181 ctcaacttct gttttcattt taggatattt gatccgagaa tggaaggtgc tacttaaacc
     6241 gctgatcaat gatttttcca cttttcggat ttctttcaag gtcagtccac tatcgtctaa
     6301 ttgaccatcg gaaatacgct cttcgataag attatggaca aaactttcga ttttttcgct
     6361 cgtcggatga tccattgccc gtactgcagc ctcacaactg tcagcaatat tgacgatccc
     6421 tgcttcacga ctttgcggtt ttggtccagg ataacggaat tgctcttctg ttacttccgg
     6481 attgcgttct ttcgctttga tgtagaaata acgcattagc gtggttccat gatgctgttt
     6541 gcaaatatcg atcaccattt gcggcatatt ataatcttcc aatattttgg ctccatctgt
     6601 cacatgtcca aagataatat gtttactgtc ttctggcaat aagaaattgt gcgggttttc
     6661 cgctcctgaa ggcaagttct caacgaaaaa gctagcatgt ttgatcttgc cgatatcatg
     6721 ataatagcaa gctactcgag ttagcaaaga acgtccgccg atttctgcta cagcgttagc
     6781 acttagatta gctaccatca tactatgatg ataagtacct ggcgcttctt ccagcaactg
     6841 tttcaataaa ggatgatttg gattgctgag ttcatttaat acaattacac tgtcatcatt
     6901 gaccatcaat tcaatatatg ggtgtagacc gatcgtcagc aagaatgaga agagtgcacc
     6961 agtgaacccg cagacaagca ttaaccaagt cgttgcatca ccaaagctca ttccttgata
     7021 gacgatcaaa atgacatcca tcatcacagg aaaaataatg acccacatgg cagcagataa
     7081 tccttgttct gagatccgtt tgcgtctgac aatcgttgcc atgagcccag agaaaacata
     7141 agacatcaag ataatcgtta gaaagttcgt gccaatcgaa tcataaaaaa tgaaaagtgc
     7201 agatactgct tggaaaatac cagccatgat ccctgctctt cggttcaaga aaaagcttaa
     7261 aacaagtggt gcaaaagcag cgggataaaa cagcgggata taagtagctt gttccgtttg
     7321 gaataattgg aaaaatttca tcagcactat gctgactgac atagctgtca catagaaaag
     7381 cacgtatttt gttctttctc ctgcattttg gaattgaagt gaattataaa tcagtacgcc
     7441 gatttgaagc aaaacagcta aaaccatggc aattagcgga aaaatcgaag ttgtttgatt
     7501 agtcagtcct agtaattcca attttttcat agctgaagca tcgatttgat taccttcacg
     7561 aacaatgatt tctccttgga agatcatcac tggttgtaca gcttctcgtg cggattgttt
     7621 caattcttct gtctttttct cattcaatac gtcattcact acgatacctt gatccactaa
     7681 gtagcgaatc atttgttgtt gtgctggtgt gacattcaaa tattggattt gctcttctgc
     7741 ttcttgttta aaagcttcca attcactttc acgaacttgt ttgctcatct gttcgtcaat
     7801 gagcttcaga ctctcatccc tcacagtatg gatctcagtt tcagtcattt caaatatcgt
     7861 cgaataaaaa ttgttcggta gtttttgata aaacgcaaca ttttctgcat tcacattttc
     7921 aaaatttttc ttcaacgcgg cgatccgctc ttctgtagta ggctttggga tattttcatc
     7981 ttcctttgcc ttatcaatct tttcgtcata agatttgttg atggcatcat tcgttttatc
     8041 gatcaattca aatagttgct tgatccgatt gttctggtca tctgcaagat ctttttggta
     8101 agtgtattct ggagtgactg cttctgctgc cagttttctt ttttgttcgg tttcttccgt
     8161 gttctcgatc gttttgttcg cacggatact ttcttctgct acttggcctt ctctgaaatc
     8221 attggccttt tgtttgacac tactaaatgt aacgccgcag ataatgataa aaaacaagaa
     8281 cagcaggaaa ggcaatgctg cctttccaag tttttcttgt agtttctgca gtgttttatt
     8341 caacatgtgc ttatcctcct aagcaaagct tattcttgat ggagatctgc cttttcataa
     8401 gctttgatga tttcggcaac tacagggtgc cgaacaacgt cacttgcttc aaaattaaca
     8461 aaatcgattt tcttgatttg ctttaatgtt cgttctgcat gaacaagacc acttgttgtc
     8521 cctcgaggca gatcaatctg actcgtatct ccattgacaa tcatttttga ttgataacca
     8581 agacgtgtca ggaacatttt catctgagca actgtcgtgt tctgtgcttc atccaaaata
     8641 acgaatgcat catccaatgt ccgcccccgc atgtaagcta aaggagcaat ttcgatcacg
     8701 cctcgttcca tcaatcggtt cgtatgatct aatccaaaaa tttggtataa agcatcataa
     8761 acaggacgca gataaggatc tactttttct tttagatccc cgggaagaaa gcctagattt
     8821 tcccctgctt ccacagcagg ccgcgtcaaa atgatttttt gcacttcacc ttttttcaag
     8881 gcagcgattg ctaatactac tgcaagaaaa gtttttcctg tcccggctgg tcctacgcca
     8941 aatacgatat catgtttggc taccgattcg acatatttct tttggccgct gttttttacc
     9001 cgtattggtt ttccattacg gtctttgacg atctcttcct catacatttc aacaaaataa
     9061 tctaatgtgc ctttttgccc cattttcagt gccgtcacga catctggtgt acttacatgg
     9121 attcctcgtt tgatcaattc ctgcaatgtc cgaatgactg aggcagccaa ggaaatctgg
     9181 gattgttcgc caatgatctg aatgatttct ccacgtgtat ggatggttgt ttctgttgtt
     9241 tcttcaatca gttttatatg cttgtcgtgt gcacctaaaa gcatgctgac atcatcagca
     9301 gccgtcaatc taatctctaa agaactagac tcttccgtca aaaatagaca tccccttttt
     9361 ttcgattcat tctttcctat tttaccatac tcagaagaaa ctatatagca aataagaaaa
     9421 ggttctaaaa gagttgctta accataagta ccaagaaacg ttaaaaaata gctgcttcca
     9481 ttctctaaag gtctcgtttg aataaaaaaa gctgatcatg taagctatgc ataagataga
     9541 ggacaagtat tccgtctaac ttagcaagta ttctctgggt atttttcttg tttcaaaaaa
     9601 agaaaacaaa atatttgaaa tattcttgca tttcaaaaaa aacaggtgta ttataacgtc
     9661 aacaaataaa tatacagcaa agcgtcgagg aaaacagtag gctattcagc gatttattcc
     9721 cagtgagtca tgtaagtgag aaatgacaat aaatcgaata gacgaacatc atttccgagt
     9781 caatgtcgaa aacgtagatc attgtggctg cctccattat caggctagcg gataacttta
     9841 tcgaaaagcg tccgtgaatg aagaagcttt tttaagcgaa aaaaggtggt accgttctag
     9901 aatctagagc ccttttccca agtggaaaag ggcttttttt tatattttaa ggtggtggtt
     9961 ttcatggaat ctgatattag tggcatttct agtttctgca acgtttattt tgttactcaa
    10021 aatgataatt attggaggaa aaatttatgg aagaaacaaa aacttattca tggaagcaat
    10081 tattgatggt cagttcactt atcttcggga tgttcttcgg gtcaggtaat ctgatctttc
    10141 ctgtccactt aggtcaaatg gctggatcaa actggatcag cgctggtgta ggatttgcca
    10201 tctcaggcgc tcttttccca ttattagcta tcttagctgt agtagtgaca aaaagtgatg
    10261 gtctgtatga cttagctaaa cctgtcggaa aatggtatgc tgccttgttc ttgatccttg
    10321 ttcatttaac gatcggacca ttctttggta caccgcggac agcagcaact gcttttgaaa
    10381 tggctgctaa accctttttg cctgaaaaat atgattcatt aggtatgttt ttattctctg
    10441 ctcttttctt tttacttgct tatttcctta ctgtacatcc aacacgtttg gtcaaatatg
    10501 tcggaaagta tttaaactcc ttgtttcttg cattattagc tatcattttt gtcgtttctt
    10561 tattccaacc catggggaat ctaaaccaat ctgctgcaga ttcttatcag acgaacgctt
    10621 tgacaaatgg cgttttagaa ggatacaaca cggttgatgc agtagcttta cttgctttaa
    10681 gtgtcacttt tgtccatgca gttcgtgatc ttggttttaa agataaaaaa gtcacagaat
    10741 taacagcaaa agctggtagc ctgagcgttg cgttagaagt catgatttat tttggtctgg
    10801 tccttctcgg tgcgatgagt ttgaataaga tgcctttatc agaaaatggc ggaactgcac
    10861 tttctcagat cactaatttt tatttaggta acttcggtgc tagcttctta ggggtaatgg
    10921 tcacattagg tgtcttcact accgctatgg gactcgttgt ttcttttgca caagatttcc
    10981 ataaactgtt ccctaaagtc agttatatga catggctgcg gttaacgact tttgtctctt
    11041 ttgtcgttgc gaatgccgga ttggataata tcatccaatg gtctttacca gtattgatgc
    11101 ttttgtatcc attgtcactt gcgttaatct tgctttcctt aacagcaaaa ttcttccaaa
    11161 agagtccatt tgtctatcaa gtaacgatgt tcttttctgc cgttccagcc gtattggata
    11221 tgttggcaaa ttcgcctgca ttagtgagtc agcaaactgt cgtagcaagc ttactaggat
    11281 tttatcatca tcacgttcct tttgctgcat taggacttgg ctggatggtc ccaacgttac
    11341 ttggttacgc tggcagctta ctcttttatt acgcctatcg cctatcaggg tataaacaag
    11401 aagcaaatga actaccagaa gaataaccaa atcaaacaca atttaacata agagatatta
    11461 aacaggaaaa
//
LOCUS       DAGCYL010000082        11386 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.083, whole genome shotgun sequence.
ACCESSION   DAGCYL010000082 DAGCYL010000000
VERSION     DAGCYL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.083"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(420..2429)
                     /gene="metG"
                     /locus_tag="MB216_001637"
     CDS             complement(420..2429)
                     /gene="metG"
                     /locus_tag="MB216_001637"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBT4526475.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2697..2975
                     /locus_tag="MB216_001638"
     CDS             2697..2975
                     /locus_tag="MB216_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526476.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            3072..3893
                     /locus_tag="MB216_001639"
     CDS             3072..3893
                     /locus_tag="MB216_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBT4526477.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4178..5188)
                     /locus_tag="MB216_001640"
     CDS             complement(4178..5188)
                     /locus_tag="MB216_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4526478.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5200..6153)
                     /locus_tag="MB216_001641"
     CDS             complement(5200..6153)
                     /locus_tag="MB216_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4526479.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6143..7213)
                     /locus_tag="MB216_001642"
     CDS             complement(6143..7213)
                     /locus_tag="MB216_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4526480.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7219..8262)
                     /locus_tag="MB216_001643"
     CDS             complement(7219..8262)
                     /locus_tag="MB216_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4526481.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8263..9204)
                     /locus_tag="MB216_001644"
     CDS             complement(8263..9204)
                     /locus_tag="MB216_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4526482.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9288..9635
                     /locus_tag="MB216_001645"
     CDS             9288..9635
                     /locus_tag="MB216_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBT4526483.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9705..11366)
                     /locus_tag="MB216_001646"
     CDS             complement(9705..11366)
                     /locus_tag="MB216_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4526484.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3218 a         2318 c         2069 g         3781 t
ORIGIN      
        1 tatctatatt ttcaatcaca catataagag cacaaactaa ttgaacataa agtgatagaa
       61 cttataatta acacatcaac gctactgtta gatataaata tagatgtaat aaagtcattg
      121 cattcaaact ttatgaatgt aaggacaata aaaaagagta ccagatgaat caattaaaat
      181 ctgcccgacc ctttatcatt atgctttttt tctgttttta cgattcctat tagtattatt
      241 ctaaattaac aaaaatcagc gaagctcgta cttctttttc taggatttat tttggatagt
      301 tctctgtttc tgtattatat gattggcttc aagtaataag aaggaattta cgaaaattgc
      361 ttctcaaatt tcggaaaaag ggggtatgac acaactagtg tcataccccc tttgaatttc
      421 tatgcgattt ctgagccatt tggcatggat tttggtgctt cgattacttg taaagagcca
      481 tctggtgctt ctgcagaaag gatcattcct tgactgattt gtccacgcat ctttcgtggt
      541 ttcagatttg ctacaatgac gacttttttc ccgatcaatt cgcttggatc aggataaaat
      601 tcagcgattc ctgacaagat ttgacggtct tggctgtctc cagcatctag tcggaattgc
      661 agcaacttat ctgccccttc tactttttga cagttgatga cttctgctac ttttagttct
      721 actttttcaa atacatcaaa tttgatttgt ttttctttcg ttgaaacaag ttctgtttct
      781 tctggatccc atttgactgt atcttcgttt gtttgagtac cttcagacat tttttcttga
      841 ataaaagcaa cttcttcttc catatctaaa cgtgggaaga ttggtgtacc ttttgcaact
      901 acttttgttc ctgaaggaaa ttcgccaaag tgtaatcctt caagattcat tgtttcagag
      961 tccaatccta gttgattgaa gatcttctct ggcgtttctg tcatgattgg ctgaagtaat
     1021 atcgctgcga tacgcaaaga ttcagctaaa tggatcatga cactattcaa ttcagctttc
     1081 ttttcttcgt cttttgctaa tacccatggt tctgtttcat caatgtattt gtttgcacga
     1141 gaaatcagca cccagatttc agccaatgct gtattgaatt ccattttttc cattgcatca
     1201 tggtaacgtc cgacaacgtt agcagccgtc gtagacagtt cactatcaaa tggagtgaca
     1261 agtgaggcat agtcaggaac gagtccatca caatatttgt tgatcatagc aatcgttcga
     1321 ttcaataagt tccctagatc atttgccaga tcataattga cacgggaaac aaaatcttct
     1381 ggcgtaaaga caccatctga cccaaacgga acagcacgaa gcaaataata acggagtgca
     1441 tctaaaccat aacgttccac aagcatttca ggataaacga catttccttt tgatttagac
     1501 atttttccgt ctttcatcaa tagccagcca tgcccaaata cttttttagg tagaggcaaa
     1561 tcaagggcca tcagcatgat tggccaatag atcgtatgaa agcgaacgat ttctttaccg
     1621 accatctgaa catctgcagg ccagtatttt tcgaatagac tttcatcttc ggagccatat
     1681 cctagtgcag taatataatt ggataatgca tcgatccata catacaccac atgtttcgga
     1741 tcactcttga ctggtactcc ccaagtaaaa gttgttcgtg aaactgcaag atcctctaat
     1801 cctggtttga taaagttgtt gatcatttcg tttttacgag attctggctg gatgaattcg
     1861 ggatgttctt catagtattc caatagacgg tctgcgtatt tgctcatgcg gaagaagtat
     1921 gattcttctt ttaccaattc tacttcgtgt ccacttggcg ctttaccacc gatcacgtta
     1981 ccttcgtcat ctttgtacac ttctgcaagt tgtgtttctg taaagaattc ttcgtcagaa
     2041 acagagtacc agccttcata ttcacctaag taaatatctc cctgttctaa taaacgatca
     2101 aatatttttt gaacagctgc tttatggtaa tcatctgtcg tgcgaatgaa tttatcataa
     2161 ctgatatcaa gcgttttcca taatttcttg acatcagccg ccattttatc tacatattct
     2221 tgcggtttta cacctagttc ttctgctttt gtttcgattt tttgaccatg ttcatccact
     2281 ccagtgagat aaaacacatc aaagcccatc aaccgtttat aacgagcaat tgcgtcacat
     2341 gcaatcgtag tatacgagtt gccgatatga agtttgccgc ttggataata gattggcgta
     2401 gtgatataaa aagtttcctt ttctgccacg gggctcttcc tcctttatcg catgatgcga
     2461 attcgtttcc ttgctagtat accataaaaa aagcatgctg ttagcttttt ttcaaaatga
     2521 aaaagactgg ataaacgact tctatctacg ttcaatctat cctttaaaag gatgaatagg
     2581 ttgaataaat gcgggttttt gttttgtttc atatctacta aaaaacggct ttttatttct
     2641 cccctcacac gtggtataat aaagactatc tatcttagta agggactgga acaatcatgc
     2701 agaagaaaca tctttttttt acacttagta ttgcttttct ttcattagca catttaatat
     2761 tcagctattt ttatattcgt atgtatggtt attttaacct ccatgggcat ttgaacagtt
     2821 ttatgacagc agcttggatt ttgcgtttta tcattgacgt atatatcgtt atttgtggct
     2881 tctttgccat tcgtgaagag cggtataaag tattgccgtt ttatctgctt ttctttttat
     2941 tcaatctcat acttccattt atttttcata tttaaatgat aacctagatt tttcaagtaa
     3001 atccttggtt ttttttcaga gtctgctaca ataaggtaaa aagtactttt actgtcaatg
     3061 gaggtggtca gatgaaccgt tttgcttcta aagaagaaga aaaattatat tatgaacata
     3121 aagcaagtga agaagaattt cttaactggt atcaaaaaca ggaacgtcca gaatatgaca
     3181 agccctcttt gactgtcgat atcgtactga tgtgctataa caaagaagaa gatcagctga
     3241 aagttttgtt gatccaaaga aaagggcatc cttatcgaaa ttcatgggca ttgcctggtg
     3301 gctttgtcca aaaggatgag tcaactggag aaagcgtctt aagagaaaca aaggaagaaa
     3361 ctggtgtggt tatttcaaaa gaaaatattg agcaactgca tacattcagt acgcctaatc
     3421 gagatccgcg aggctgggtc gtcacagtca gctacttagc ctttattgga gaagaaccgc
     3481 taatcgccgg agatgatgcc aaggaagtac gctggttcac actagaacgt catagtaaca
     3541 agatccattt atctagcgga gaagtggcta tctcgcttga tttagtcact ggagagtctt
     3601 ctggcaaaga tactttggct tttgaccata gtcaaatcat cttgaaagca ttcaaccgtg
     3661 tagtcaataa aatggaacac gagccgcaag ttctacaagt tttaggtaaa gatttcacga
     3721 ttacggaagc tcgaaaagta tttgctaaat tcttagggat cgattataaa acgatcgatc
     3781 attccaattt taagaaagct atgctccaat atttcgatga gatcggtgaa cgtcctgtag
     3841 gaatcggccg tccttcaaaa atctatcagc tcaagccggg gcaccatgaa taaggttgtg
     3901 agcaaaagat tcagacctaa tcattcaaga aaaaacgcaa aaacaactct ttgtgagtta
     3961 cgttttttgt ttcagaattt tgatgtattc gtaaactttc tgaataaaaa attaacaata
     4021 gcagaaaaag ctcgtacgaa ctttttctgc tattgtttta ttggtttgtc ataattagtg
     4081 agcttttctt caaaagaaaa attcctagaa acaaaagtca ttaagacttt ttattaacaa
     4141 ggctaaccct aacgctccta tcagtttcat tcatttttta tcccttgata accgaatgtc
     4201 tgcccatttt ttctactact tggctctttc ttacggaatt gctttcttct gctccgtcgg
     4261 tattgatcaa aagaaatttt ttattatacc attgggactt ataacgcaat tttgttatta
     4321 atttatgcca gtcgtgaata gaaaaatcag catgtgcctc ttcttttgga aaagattcta
     4381 caaataagta gtcatattgt ttgaccaatt tcccagataa ttgttcaaga taatcttctc
     4441 gctggttctt ttgtttcaga taaagatcca ttacttttct cttttgtttc tgataattgc
     4501 ttgcctgatc taacctgatc tttctatggt gagcggagcg gactttcaca ttcaatttcc
     4561 gctgtgcatg ttgcagcttt tcttctgtct ggtgaaattt tggtaaagtt acttctagag
     4621 gtcgagatgt ttggatcaat gtcttcggat gataggcgat cccgatccat ttagctaaag
     4681 attctttttt tactggcgaa acatcacaaa gtagtgcgac ataaaattct tcattatatc
     4741 gagcagaaat cgtagcggag cgaatcgatc cttcaactgg ccgatgctga tttatcttga
     4801 ttagttcttt ttgttttggt aactttaagt agccattttc cagatagatc gtacgggttt
     4861 ggttgttggt cgtataagat tgccacatac tttttttcgt tttaagcttt gggaagctgg
     4921 cacgtccctt gaaataattg cgaaaagccc gatccaaatt aagctgtgca tttgctaaag
     4981 caagactgtc tacttctttt aagaatgggt attctttttt taatgtagcg ggtgtgagcg
     5041 aaaaattggc tactcctgtt tgttgccaac ttttctgccg ctttttcagc aaatggttat
     5101 aggtaaatcg agcacatcca aaagtacgaa tcaattgctg tttttgttct tctgtcggat
     5161 acatacggaa tttaaaagct tttagcatct atgtccctcc tagttagcaa ccagaagaga
     5221 ttccttttgt cgttttagtt tttttgcgta atatgggact tcctgctcag ttgcatagac
     5281 aaaatgttct ggtgcgattt cacgaagttg acgtacttgt ccgtcatctg gttcaggttg
     5341 atatttgatt cttctgcgtt ttctctcatg gtctggatct ggtagaggaa tagcagataa
     5401 caaactttct gtataaggat gaacaccgtg attatagatt tcatcacttg ttcccatctc
     5461 taataacaag ccgttatgca ttacaccgat tcggtcacta atatgtttaa ccatcgacaa
     5521 atcatgtgca ataaatagat aggtcaattg atgttctttt tggagatctt gcaacaaatt
     5581 gaccacttgg gcttggatcg acacatctag tgcagaaatt ggttcatcac aaatgataaa
     5641 acgcggtttt actgccaatg ctcgtgcaat accaatacgc tggcgttgcc cacctgaaaa
     5701 ttcatgagga taacgagtac cgtggcttgg attcaatcca acagttctca atagatcatc
     5761 gacttttttt gcacgctctt cagatgattt agctagtcca ttaacatcaa ttccttcggc
     5821 aatgatatct ctgactttca tacgaggatt caacgaagcg tatggatctt gaaagatcat
     5881 ctgaacatca tgccggaatt tcgtcaaacc ttttttatct ttgatcttcg ttatatcttg
     5941 accgtcgaat aatatctcac catcagtagg ttcattcaag cgaatgatcg tacggccagt
     6001 agtggatttt ccgcttccag attctccaac taaaccgaat gtttctcctt cataaatgtg
     6061 aaagctgatg tcatttacag catgtacttc attctttttt cctacattga aatactgttt
     6121 caatccttta acttcaagta gtttatttgg catcagcaat tcctccttga ccgtaagtat
     6181 gtttttgttt ctctttaaag atagcccagc gacgttggat ttcttctggc ggtgtcactt
     6241 tgggtgcctg cggagcaagt agccaagttg ctgccttgtg tgtttttgat agttcaaaaa
     6301 atggtggttc ttgttctgca tcgattttca tcgcgaattc atttcttgga tagaatgcgt
     6361 ctccttttgg cgggttcaat aaatccggtg gagaaccagg aatagcatac aatcgatcgt
     6421 tcgcactatc taatgtcggc attgaaccaa gcagtcccca agtataggga tgttgcgggt
     6481 tgtagaaaat ttcttccgat gttccaactt caacgattct tcctccatac atgacagcga
     6541 cacgatcagc gacattcgct actacaccaa ggtcatgagt gataaagata attgatgtat
     6601 cgatttttgt ctgaatttct ttcagcagct ccaaaatctg tgcttgaata gtcacatcaa
     6661 gcgctgttgt aggttcatca gcaatcagga tttccggata acataccagt gcaatcgcga
     6721 tcacgatccg ttgacgctga cctcctgaga actgatgcgg ataactgttg atacgtttct
     6781 cagcttcggg tattccaact aatttaagca attctaatgc agctttttgt ccctcttttt
     6841 ttgaaacttt attatgcttt ctaagggact cagcgacttg cttcccaatg ggcatcgtcg
     6901 gatctaaaga ggtcattgga tcttggaaaa tcatcgctat ttctttacca cggatttttt
     6961 gcatctctct ttctgatttt ccaacgatat cgttaccttt aaagaggatt tgaccagcat
     7021 ctatattggc gttgcttgac agcaagcgca tgatgcttct ggtcgtcact gattttccac
     7081 ttccagattc cccaacgatc gccaatgttt ctcctttatt caaatgaaaa ttgacaccgc
     7141 gaatcgctcg tacctttcca gcatacgtat caaaagagat ttctaagtct ttcacttcta
     7201 aaattttcgt catcgtcttc actctttcat tttaggatca aaggcatcac gcaatccatc
     7261 agcaagtaag ttaaagccaa tcatgataac cgataaagtt actgccggaa tccataatag
     7321 ataaggcaga tacaggaatg tcttgtaccc ttcgctcaac atcgttccta atgaagctga
     7381 cggaggcgta agacctaaac cgataaagct taaaaaggct tcgaagaaaa tagcagatgg
     7441 aatactaaac atcatttgaa taataatgac actagagata ttcggcaaaa tatgtttgaa
     7501 tgcaattttc attcttgatt cacctaatgt caatccagct aaaacatatt cttgatcttt
     7561 taatttcatt gtttgtgcac gaacgatacg tgccattggt atccagtttg tgatggccat
     7621 agcagccacg attgaaaaaa ttcctggttc aaaaacgacc aacatcaaaa tcatgacaac
     7681 gaggtttgga attcctgata agatttccaa aaaacgttgc atcacgttat cgacacgtcc
     7741 gcctaacaaa ccagaaacca acccgtaagc tacaccaatc gttacatcta gtaatgcagc
     7801 aataaatgcg atcagcaacg agattctcat tcccataaat aagcggctta atacatcacg
     7861 tcccaaaccg tctgttccta aaaagaaatt tacgtttgaa gggacgtttg cttgagcata
     7921 tttatcaaca agttcaccac caaccattgc ttttccattc aatccatcga tattgattcc
     7981 cggaatacga ggaggcaaat tgatataagc cacgttttgg gcagttggat catgaggaga
     8041 aacaaagatt gtgatcacag agatcaaaat gatgaccaat agtaaaacca ttgaaataac
     8101 ggcagctttg tttttcttta aacggcgcca tgaatcttgg ataaaattca aagaaggtgc
     8161 agtaatctgt tcacgttctt cttcggtatt tttttgtaat ggaagaaatt catccgcagg
     8221 gatatctgca actgtttcat aacgctttgg tatcatttcc atttatcctc ggctcccttc
     8281 tgaaacacga attcttggat caactagacc gtacaataga tccacgatca aaataacaaa
     8341 aatcagcata aatgagtaaa ggatcgttac agccatgatc gtaggataat catttgttgt
     8401 gattgactta acgaattgtt ccccgatacc aggaatagca aagatatttt ctaccaccag
     8461 tgatccggtc atcaatgcta cagctaacgg tcctaataaa gtcatcaatg gaatcaaact
     8521 atttcgaaga ccatgtttaa aggcgatttc ccaacggctc aatccttttg cttttgctaa
     8581 ttccacataa tcactatgca gtacttccac catttccgta cggataaacc gagcagagtc
     8641 agcaagcggc gacatggcca acgcaattgt tggtaagatt gtgtacacaa agccatccca
     8701 ttttgcgatt ggcaaaactt gtaatttgat tgcaaagata tattgtaata aaacagcaaa
     8761 tacaaagttt ggaatggaac gacctaatat cgctacgagt gtagaagatg tatctgccca
     8821 tgtattttgc ttcatggcag agatcgttcc taaaatcgtc cctaggactg ttccaaagat
     8881 aattgcctgt aaccctaact gcaaggatgg tccgattctt ccagctaaca aatgtgcgac
     8941 tggctggttt ttaaattgga aggaaattcc aaaatctcct tgaagcaaat tagaaagata
     9001 aatcccatat tgtacgataa ctggtttatc caaaccgact tgtttattta gcatctcgat
     9061 tgtttcagga ctcaaacgtt cctgattcgt atatggcgtg cccggcagca attgcatcaa
     9121 gaaaaaggtg attgttgcaa ttaaccaaag cgtgatgatc atgaaaaaga cacgttttaa
     9181 tacatatttg atataactat tcactaatgt ttccccctag cttttgactt ctgacagatg
     9241 tttcgctaca ttattagata atgaaccaaa aataaaggag caatcaaatg aaaaaaaaat
     9301 ctatccccta cgcagtagct tttctactga ttctggttat tttaataaaa aatctcatca
     9361 accattcctt tacactcatt caattatcta atgatctatt cttatggtca ttgccgtttc
     9421 tgattatcgg cggtttttta tgggtctttt cttcgggttt cttcgatcat tttcagcgat
     9481 cggtccatct tgcacgaaca aggaaccgaa agaaaaaacc agaattttcc tcgctctcgt
     9541 ctgccagcta cggtatgtac agcttttggc tgatcattgc cggaatacta attgctcttt
     9601 ctgctatttt catgcttttt tctttgttag gataagtgaa tatgagaacg aggttgcgac
     9661 atttgccgca gcctcgtttt ttctcatgct cacggtatta acgattattc ttcaacgtat
     9721 gtccatttgt aatcccatga agcgcctgcc gcatggtgaa cgataccttt gattttttca
     9781 ttcactaggt gaccttctgc tttttggtac accggaacga cacccatatc atcagagatg
     9841 atcttgattg ctttttggaa atcatcccaa cgggcttgtt catcgccagc gtctgttgat
     9901 gaagctgctt taactgcttc gtcatattct gcattgctcc agcgaccacg gttgtatgaa
     9961 ttacctgtga caaacaaatc agtaaagctg cttgggtctg catagtcagc agcccatccg
    10021 cctagtactg cgtcaaaatc acccgaagtt gagcggtcaa gacgtacaga gaatggaaca
    10081 ggtgttggtt tgactgttac gccatccaag ttttcttgga ttgagttttg aacatattcg
    10141 attacttttt tcgatgaatc atcatcagaa gccatcaaat caaattctaa agaatcaacg
    10201 cccaattctt ttttcgcttt ttcccaatat tcttttgctt tatctttgtc ataagaaact
    10261 aattttccag cttcttttgc aaagtcttcg ttcgttgttg gactttttac catatctgat
    10321 gggatcaatc ctgtagagac aactgatccg tcacctagta cttgttttac taaagcttca
    10381 cgatcgatag aataagaaat agcttttctt aggttcacat tgttgaatgg tgaattcttt
    10441 tcacgttgat tgaattcgat atagcttgtc cgcgcttctt taacagaagt ataagctgga
    10501 tcgtttgcgt tttgctgtgc taactcacca gacaagatca cgtcatctgc ttggccatct
    10561 ttgaataagt tcaatgcagt agaagattct tttactacac tgactttgat ttcagaaagt
    10621 tttactgtat ctttgtccca gtaatgatca tttttcttat aagtccattc tgtatctgtt
    10681 cctggtccat caaaatcttc taatgtgaat ggtccattat aaacagcttt atcacttgca
    10741 gatgcatagg catccccatt ttcttccact acggattgtt tttgtgggaa aaatgatggg
    10801 aaagcaagca gatattggaa atatggtgtt tgtttcgtca atttgatttc taattcatag
    10861 tcaccaactg ctttgatccc tagttctgat ttatctttct ttccagctgt aatgtcttct
    10921 gcattttcaa ctggcgcata caggtaagca tattcagaag ctgtagctgg gtctactgtt
    10981 cgttgccagc cataaacgta atccgctgct gtaacaggat caccatttga ccattttgcg
    11041 tcttctcgta atttgatttt gtaagttaat ccgtcttcac tgacttctgc caactcgcta
    11101 gctcctgccg gctgtggttt gctgtcttca tcaagacggt aaattccttc ataaacgtta
    11161 ttcaatgctg taaaactgat tgtatctgtt gcaactgata agtcagctgt aggcatttct
    11221 tgaggcacga ctaagttaaa tacgcctcct ccatctgctg aaccgctttt tgctgaacta
    11281 ctgcttgtgg ctgaactgcc gccataacat ccagcaagta caaggccgca taccgcaacc
    11341 aaaccaaatg taaatttctt tttcatttta cgtacccctt tattgg
//
LOCUS       DAGCYL010000083        11211 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.084, whole genome shotgun sequence.
ACCESSION   DAGCYL010000083 DAGCYL010000000
VERSION     DAGCYL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.084"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            6..305
                     /locus_tag="MB216_001647"
     CDS             6..305
                     /locus_tag="MB216_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4526485.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="MB216_001648"
     CDS             378..1739
                     /locus_tag="MB216_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBT4526486.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="MB216_001649"
     CDS             1761..2021
                     /locus_tag="MB216_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526487.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="MB216_001650"
     CDS             2035..2787
                     /locus_tag="MB216_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBT4526488.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="MB216_001651"
     CDS             complement(2881..3486)
                     /locus_tag="MB216_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4526489.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="MB216_001652"
     CDS             complement(3539..4513)
                     /locus_tag="MB216_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBT4526490.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="MB216_001653"
     CDS             complement(4891..5610)
                     /locus_tag="MB216_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4526491.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="MB216_001654"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="MB216_001654"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBT4526492.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="MB216_001655"
     CDS             7161..8558
                     /locus_tag="MB216_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4526493.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="MB216_001656"
     CDS             8639..9079
                     /locus_tag="MB216_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBT4526494.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="MB216_001657"
     CDS             9227..9553
                     /locus_tag="MB216_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4526495.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="MB216_001658"
     CDS             9554..10303
                     /locus_tag="MB216_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526496.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELIGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="MB216_001659"
     CDS             complement(10383..10838)
                     /locus_tag="MB216_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBT4526497.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3557 a         1960 c         2300 g         3394 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg ataggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt a
//
LOCUS       DAGCYL010000084        11190 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.085, whole genome shotgun sequence.
ACCESSION   DAGCYL010000084 DAGCYL010000000
VERSION     DAGCYL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.085"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(254..1102)
                     /locus_tag="MB216_001660"
     CDS             complement(254..1102)
                     /locus_tag="MB216_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4526498.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1101..2168
                     /gene="gnd"
                     /locus_tag="MB216_001661"
     CDS             1101..2168
                     /gene="gnd"
                     /locus_tag="MB216_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBT4526499.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2170..3171
                     /locus_tag="MB216_001662"
     CDS             2170..3171
                     /locus_tag="MB216_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBT4526500.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3168..3452
                     /locus_tag="MB216_001663"
     CDS             3168..3452
                     /locus_tag="MB216_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBT4526501.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3485..4282
                     /locus_tag="MB216_001664"
     CDS             3485..4282
                     /locus_tag="MB216_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBT4526502.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HVAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4296..5129
                     /locus_tag="MB216_001665"
     CDS             4296..5129
                     /locus_tag="MB216_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4526503.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5147..6004
                     /locus_tag="MB216_001666"
     CDS             5147..6004
                     /locus_tag="MB216_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBT4526504.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6026..6535
                     /locus_tag="MB216_001667"
     CDS             6026..6535
                     /locus_tag="MB216_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4526505.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYSVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6572..6973
                     /locus_tag="MB216_001668"
     CDS             6572..6973
                     /locus_tag="MB216_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526506.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7085..7438
                     /locus_tag="MB216_001669"
     CDS             7085..7438
                     /locus_tag="MB216_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526507.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7472..7915
                     /locus_tag="MB216_001670"
     CDS             7472..7915
                     /locus_tag="MB216_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBT4526508.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7932..9017
                     /gene="uxuA"
                     /locus_tag="MB216_001671"
     CDS             7932..9017
                     /gene="uxuA"
                     /locus_tag="MB216_001671"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBT4526509.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9010..9723
                     /locus_tag="MB216_001672"
     CDS             9010..9723
                     /locus_tag="MB216_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4526510.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9930..10079)
                     /locus_tag="MB216_001673"
     CDS             complement(9930..10079)
                     /locus_tag="MB216_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526511.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10475..10675
                     /locus_tag="MB216_001674"
     CDS             10475..10675
                     /locus_tag="MB216_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4526512.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3764 a         1747 c         2408 g         3271 t
ORIGIN      
        1 gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact actgtatact
       61 aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct tctccaaaga
      121 tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc ttgcagaacc
      181 aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat ttttcgcttt
      241 ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta gcgatagaac
      301 gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc aaaatgctta
      361 gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca gaaatagaaa
      421 taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta ataacgattg
      481 tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt gattgccctg
      541 tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc atcaattgaa
      601 tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta tgataagcgt
      661 cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta gattcattga
      721 taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg tcatttaaag
      781 ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct gttttctgaa
      841 tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg gccattttaa
      901 aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa
      961 tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt ggattttcta
     1021 aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt gttttgattt
     1081 gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt atcatttttt
     1141 ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca aaaaatattt
     1201 ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga aatggaggcg
     1261 ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta gctttaaact
     1321 taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct gttagtcagg
     1381 caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct acttttgagc
     1441 agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac atgttaagaa
     1501 aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac tcttattata
     1561 aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac ttggattgtg
     1621 ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt ggaggagata
     1681 aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa agaggatatc
     1741 tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat ggaatcgaat
     1801 atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt
     1861 ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc ggctggctga
     1921 tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat cggggaatcg
     1981 tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg gctgttccag
     2041 ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa gatagttttt
     2101 cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg
     2161 gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt cggaagagtc
     2221 tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt tactgttgat
     2281 caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat cattattgca
     2341 agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact gctattgatt
     2401 tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga acaccagaca
     2461 atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa taatataact
     2521 aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa ggacgatcga
     2581 tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt tggtgtgatt
     2641 ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt tcgctgtgat
     2701 gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa
     2761 gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc gaatttgaca
     2821 gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga aaatgtttat
     2881 ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa tggtctgaaa
     2941 agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg acgtcaagat
     3001 catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc ctacacaagg
     3061 gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac
     3121 cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg atgaagaaaa
     3181 tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa gaaggtggta
     3241 tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg gtcacccacg
     3301 gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac gaaacgattc
     3361 aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga cgaatagcta
     3421 caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa aactgaccga
     3481 tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc ctactttagc
     3541 tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg gacaagcaga
     3601 agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc cccaattagt
     3661 gattgattcc cctgttccat atttcattgc cgggttagca gatacactag caaaatggta
     3721 tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt tgaagctagc
     3781 acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta aagcaatcca
     3841 agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga ttatctttgc
     3901 tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgcgg cagcacatgc
     3961 tatgcatgtc gctatcagta aatatattcc ggaaagccat cgttttctac atggagaaaa
     4021 agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa aaatagacga
     4081 gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa tgggattatt
     4141 tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga aagagaaagt
     4201 gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga tggaactgga
     4261 aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt gtagtacaaa
     4321 gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca
     4381 cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg atcatggggg
     4441 aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg ggggtttttt
     4501 ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca gtaccagtcg
     4561 cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca gtcggattgc
     4621 tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt aaatatacag
     4681 acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt ctttatccaa
     4741 cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat ttaggggcgc
     4801 cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac attcttgaag
     4861 tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa
     4921 aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg aaatctttag
     4981 atattacgat ggttacttat gccatttttg ggattattat cgctttgatc tttgtccagt
     5041 cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca tcttttgatg
     5101 acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg ctgaaataat
     5161 ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct
     5221 gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg cgccatcact
     5281 actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact ctttaaaagc
     5341 agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg gcggtgtgat
     5401 tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg aagaaaaaga
     5461 gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg gggcactagc
     5521 aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg caatcgcggt
     5581 accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct gctttgggtt
     5641 gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg gccgtaatgc
     5701 tacaggccta atgcaaaaca ctggtgttca gacagcgatt gaaatgctat cagttttagg
     5761 gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt
     5821 tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg tgccagggat
     5881 attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag gattgaaagt
     5941 cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa tcggtatttt
     6001 ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg
     6061 tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga attccatttt
     6121 tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca aaaatgcagg
     6181 aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat tctattggga
     6241 tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc aaacgattta
     6301 cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga ttgctcaaaa
     6361 aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat cagatgctga
     6421 aaaattgaaa gaaatgaatg aacaatatag cgtaaagaat atttattttc aagccactcc
     6481 atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt cataaacaaa
     6541 tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg
     6601 ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt
     6661 tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag tcattgctta
     6721 cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa aagcagagaa
     6781 agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt tcatgtttga
     6841 ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca ttttgttgtt
     6901 cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg tattcgatct
     6961 gctatccgta taaacaggta tcactgtact agcatttggg agctactttt tcttattggg
     7021 aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt catccatatt
     7081 aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg cgtttgttga
     7141 tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga aaaaagaggc
     7201 tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt cgggaaatta
     7261 tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa
     7321 ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca aaacagattt
     7381 acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt ttcgataagt
     7441 aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg ttacacatgg
     7501 taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat cgagccatat
     7561 tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga aggtcaaaga
     7621 aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc taattagcgc
     7681 aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa agaaagcagc
     7741 cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca atcatcagat
     7801 gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa aagataattt
     7861 aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt tttagaagga
     7921 aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat tcgatcccat
     7981 taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg ctgactaaaa
     8041 tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct gttgagagcc
     8101 aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc aaggcaggga
     8161 cagatgaacg agattactat attgataact atatacgaac aatcaaaaac ttgtctcgat
     8221 gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg aaaactgatt
     8281 tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa atcgtcgatc
     8341 agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga ttcaaattgc
     8401 cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa
     8461 tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg attccagtgt
     8521 gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg gaactttttg
     8581 gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat gcagtagatt
     8641 ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt
     8701 tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc agaaacgtgg
     8761 agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg
     8821 atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct atacgccctg
     8881 atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg tatgatcgtg
     8941 caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa aaaccactgg
     9001 gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct tttattagaa
     9061 actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga ttatgaaatg
     9121 cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag agaacaaatg
     9181 ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat caatcaatat
     9241 aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc agaagaaatt
     9301 ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa tacacgggta
     9361 aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca aatcatttca
     9421 ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa
     9481 atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg gatcgaagca
     9541 gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg gttgcccgat
     9601 cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa taaagcagac
     9661 tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc
     9721 taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac tgagggaaca
     9781 gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt gaacaattta
     9841 gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa gagaagaacg
     9901 agaatcgtcc atatgagggt ttccactcct tatgcagatt tttttaattc ttgcactgct
     9961 tgttcgatcg taacacctgt cgctacatga ttggtatttt ctttgtcaaa aacgacaaat
    10021 agattcaaat ctgtatccaa ggttactcga tattgtatat ctttgttata gaatgtcata
    10081 gccattctcc tttctttaaa tacttgctga aaggcaacga acgcgttgat aacgaaattc
    10141 catttataag tataccacca attgaaaggt tttcatagtt aagaaatttt aaagtcaaaa
    10201 gttgcagata aaagcaaata aacaagaaaa caaaaagtga atcaatcgcg gttattcaac
    10261 tttttcttcg attcgaagac aatagaagac aatgaatttt atatttcatt gcattatcct
    10321 tgtgttatta gaaaaagcat gatatattaa atgagtaatc tattttgaaa cgtaatttga
    10381 gcgatatatc tacatagata aaactccttt tgcctatcaa atatttattg caacgaaaaa
    10441 acgtacattg atacgtatca ggaggaattt atttatgaat aacggtacag taaaatggtt
    10501 caacgcagac aaaggttttg gctttatcac tggtgaagat ggaaatgatg tatttgcaca
    10561 tttttcagct atccaaggtg acggcttcaa aactttagaa gaaggccaag cagtgtctta
    10621 cgatgttgaa gatggtcaac gtggccctca agcaacaaac attgttaaag cttaataaaa
    10681 attgactttt aaccgtcttt tcgaaagaaa agacggtttt tttgtacgaa aaaattcatt
    10741 ggtcgttgaa tgggctgaat agtagtgtat aaaacaattt tgtggagcgt gaagctttta
    10801 tggtagagga aaatacgaat aaagtgatgt tgataaaaag attcctatct tgaatatgaa
    10861 tcgagatgtc ttgaaaatga atgagtttga ccaatcatta tcctatcgaa atttacagtt
    10921 tcgttcgatt ggtctgtcaa ttaatgaaaa agtaaaaaag aatatgttct aggaagaggg
    10981 cggcacatct aaatttttaa gtaacggaca gttcaaatat caagcattga aagaggctat
    11041 cctactgttt gaaggaatac gaatatataa aaacgacaaa gaggaaaaag tagttcatta
    11101 aatcccttca agtgatgaaa gaatgagatc taataaaacc tgatctttca tctgtttttt
    11161 taataacgta ataatttttt tattttttgt
//
LOCUS       DAGCYL010000085        11132 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.086, whole genome shotgun sequence.
ACCESSION   DAGCYL010000085 DAGCYL010000000
VERSION     DAGCYL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.086"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            59..814
                     /locus_tag="MB216_001675"
     CDS             59..814
                     /locus_tag="MB216_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HBT4526513.1"
                     /translation="MKELIKVTTNEENEQLVNGRELHEFLEVATEYKKWFSRMAEYGF
                     VENIDFVRVTQKCPTPGGIQNITDHAMKLDMAKEISMIQRTEKGKQARQYFIEVEKEY
                     KQQLLDTSKLSPELQMFQGIFNAVAKQELETKRLATQMENITEIVALNTTDWRRECRK
                     LVNKMAETQGGYGAYQEIQTAIYEEVDRRAGSSLKTRLTNLRNRMAGEGVPKSKRDKT
                     NKLDVIESDKRLKEIYLSVVKDFAIKYGVWKEK"
     gene            835..1101
                     /locus_tag="MB216_001676"
     CDS             835..1101
                     /locus_tag="MB216_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526514.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALNQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLVDHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            1115..1387
                     /locus_tag="MB216_001677"
     CDS             1115..1387
                     /locus_tag="MB216_001677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526515.1"
                     /translation="MTEITITDGHMSHTVKTKEEAQRIIDRYFPEGESTMQIRKPIVE
                     RSSSKMLLASIAEDKEPVYKVRQLQALCYISLVLNVILLSVVFAVL"
     gene            1406..2074
                     /locus_tag="MB216_001678"
     CDS             1406..2074
                     /locus_tag="MB216_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526516.1"
                     /translation="MSYNKWTREEEQLLIQNVRYDHRGFVCNCNELAELLGIDKKRIW
                     PKIHQMCKKDLIDEVYWDDPIEPPNKRYSQQEDKKIISMYQSGCPVTIIAQELNRTEN
                     AIRCRLNIMKKNGQLESNRKRRYTKREVELLISEIKFDKNGYVLNIDYLTRLLHRPKH
                     ELFRKICLLRKQGEITIKPDRTKASQNWYDVMKKQIDNHHKLIAARYSYQKKTRSPAS
                     KIVS"
     gene            2106..2435
                     /locus_tag="MB216_001679"
     CDS             2106..2435
                     /locus_tag="MB216_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526517.1"
                     /translation="MNEKIQNLIKELVAECANDDLGMSVSVVDEKGEVVLGQAGNDSL
                     VAWSVYRQYEKTKRDLQNNNCNCETHSTLKQLFGIEYEDPEFEEWYQDFLRFAEKMDR
                     KKGGIKF"
     gene            2435..2659
                     /locus_tag="MB216_001680"
     CDS             2435..2659
                     /locus_tag="MB216_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526518.1"
                     /translation="MISVKGLGDEMFEAMMHKAQQDVQDKILTAAKYGQTSCTVRSKG
                     LTPSFLAALESEGISNIQREEGSVKLFWEF"
     gene            2670..3020
                     /locus_tag="MB216_001681"
     CDS             2670..3020
                     /locus_tag="MB216_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526519.1"
                     /translation="MSVKDVAKKQILKMEHIIDQINEAEDLLISLKSPALNNLNELTT
                     DISIGVPTQFLGRGFFQEEQSRWHKVQLERDLGITEIQEEIRNLVSKSVQKRIEALES
                     ELRAMIYYRGDHDE"
     gene            3013..3207
                     /locus_tag="MB216_001682"
     CDS             3013..3207
                     /locus_tag="MB216_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526520.1"
                     /translation="MNDFDSLGARQQPPNEASPVGVDWQGNPLYPGDTCYLTEEGYVP
                     VDDILEYAQQHYPKIELGGI"
     gene            3210..3809
                     /locus_tag="MB216_001683"
     CDS             3210..3809
                     /locus_tag="MB216_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526521.1"
                     /translation="MDKSIRTDSDIVNHIKRLNLDGFSAGRSLVGNPATIADEKTIGL
                     FKEIKKVVKENGASYDQTRKALIMVDESFYQNLLKKENPFKEKQAEPINEPKKEDINV
                     TREDLESAELTIDPDLPGKLEKELRELSKEFFNKILASKKELLPSMKEEELVFLHKKL
                     NEFRRETPSDGFDANFHIGQILTDVILIVFAGDDLKYAD"
     gene            complement(3793..4143)
                     /locus_tag="MB216_001684"
     CDS             complement(3793..4143)
                     /locus_tag="MB216_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526522.1"
                     /translation="MYKLKKDNYNELSGICRSVMQNIKDLEEGSKMDLFDPRISDEEY
                     YLYSSVLKSYNSLGNQLLSNWETLKFYDIDAFFTKLSTYVEYLDIFIKEPTIENQLRL
                     TSIYDSLDKLNQRI"
     gene            4197..5219
                     /locus_tag="MB216_001685"
     CDS             4197..5219
                     /locus_tag="MB216_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBT4526523.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLANGKETTTVMTKKQIDQSWAKSKMKGSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDVTPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQDEVTRVADVPGHSEIEQAHLIENEDTDPIQEELLDIPDFGREEGVDDVSEFE
                     DDEYPF"
     gene            5182..5997
                     /locus_tag="MB216_001686"
     CDS             5182..5997
                     /locus_tag="MB216_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HBT4526524.1"
                     /translation="MSQNLKTMSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWSPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTAWKGKIDCLNVEEEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLYQQYGKSFMPIIAAVSKQTPSEARLITI
                     DQDNMDYELVMLKEKIERIVRVKNGEEKPNHCGLCEYCRGNLPITGFTSMDDL"
     gene            6013..6792
                     /locus_tag="MB216_001687"
     CDS             6013..6792
                     /locus_tag="MB216_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4526525.1"
                     /translation="MAEGWVKLHRSITENWIWDNPQYLKWWLDLILMANHKEKKILFN
                     GSFKKVDVGQRITSEQKLAERWGVSRNTVRKFLSLLVEDDMISIEKSRKNGTTYKVNN
                     YAIYQGFSEEKKQQTEQRAEHQKDNELNINKNEKNEKNDKNNNNLRNSRKTREYADDD
                     PNKKLAILLLKLIRKNQNIKEPDLDKWANTIRLTIESDKRTGREVQDMIVWATSNDFW
                     SGVILSPTSLRKHFDKMAIQKNKRTQQNISNDELPETGEDW"
     gene            6792..7640
                     /locus_tag="MB216_001688"
     CDS             6792..7640
                     /locus_tag="MB216_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4526526.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVSGSTIHAVITGRTGAGKTHLGAAVIKEVMMASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKTDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            7637..7879
                     /locus_tag="MB216_001689"
     CDS             7637..7879
                     /locus_tag="MB216_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526527.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            8022..8477
                     /locus_tag="MB216_001690"
     CDS             8022..8477
                     /locus_tag="MB216_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBT4526528.1"
                     /translation="MATRSKYGNKKHEVDGITFDSKAEARYYMKLKRNGMSFMPLSET
                     YCAMQENVLLQEGYLCNDRKIAPIYYRADFVIYENGQVKKVIDVKGYQDAISMLKMKM
                     FAHRYGFPVTFAKFDSKINKFIEMDCFESARQQRKRQTERRKKKLMEGK"
     gene            8479..9054
                     /locus_tag="MB216_001691"
     CDS             8479..9054
                     /locus_tag="MB216_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526529.1"
                     /translation="MTKQVNFRPELKKVTSKSNGNTEVLLVVSNGSLRGSTENLTEFL
                     GSTVTVVIQPETIEYTVPVNKQTKKPNVEYVVNADGTIDMLKEEQTSLDVGDGVEEVE
                     NVKILVSKETIDEFIKTATTLQLPENVTVNIRDVLIRLAEGDSMSEIAADHELSEIAL
                     IDQIELARHYFAPYADSWSKHKDDIIFPEEQ"
     gene            9051..9239
                     /locus_tag="MB216_001692"
     CDS             9051..9239
                     /locus_tag="MB216_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526530.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMDQ
                     KILDVGLLLLHLAEKNLI"
     gene            9247..9453
                     /locus_tag="MB216_001693"
     CDS             9247..9453
                     /locus_tag="MB216_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526531.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEASQWLLKTYTNNKKLRKKCPDL
                     FLKDDQIMPEPMILTSKEGGKCES"
     gene            9617..10015
                     /locus_tag="MB216_001694"
     CDS             9617..10015
                     /locus_tag="MB216_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HBT4526532.1"
                     /translation="MIPRFRAYSKEENEMYYPHNDKNVDWTIDDETGFIAPLVNLGGG
                     MWGMIDKYELMQSTGLKDKNGVDVYQGDIIRCTRGCHHEVIWLEEYGGIFFGGMPAWY
                     LSGLSNGYAWTGGEEVIGNIYENSELLEEK"
     gene            10015..10314
                     /locus_tag="MB216_001695"
     CDS             10015..10314
                     /locus_tag="MB216_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526533.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMKCDKWLAKSKENLAVVRLVDMEVINNEI"
     gene            10304..10462
                     /locus_tag="MB216_001696"
     CDS             10304..10462
                     /locus_tag="MB216_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526534.1"
                     /translation="MKFKILEEDTRYELEEKLNEFAKNNEIQHISLTAFMAGYTAYYA
                     AVVSYVSQ"
     gene            10560..10976
                     /locus_tag="MB216_001697"
     CDS             10560..10976
                     /locus_tag="MB216_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBT4526535.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKYGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         4162 a         1701 c         2398 g         2871 t
ORIGIN      
        1 atttttaata aaataactta tggaaaacat atttattcat ttttaaggag tgtaatccat
       61 gaaagaacta atcaaagtaa caacaaatga ggagaatgag cagttagtaa acggcagaga
      121 attgcatgag tttttggagg tggcaacaga atacaaaaaa tggtttagtc gtatggcgga
      181 atatggtttt gttgaaaata tagatttcgt aagggtgacc caaaaatgtc cgacccctgg
      241 aggaattcag aatattaccg accatgcaat gaaattggac atggcaaaag aaatctcaat
      301 gattcaacga acagaaaaag gaaaacaagc gcgtcaatat tttattgaag tagaaaaaga
      361 atacaaacaa caattacttg atacttcaaa gctaagtcca gaacttcaaa tgttccaagg
      421 aatatttaac gcagtagcta agcaagagtt agagacaaaa cgtctagcaa cacaaatgga
      481 gaatattact gaaattgtgg cattgaatac aactgactgg cgtagagaat gtcgaaagct
      541 cgtaaataaa atggcggaaa ctcaaggcgg atacggtgct tatcaagaaa ttcaaacagc
      601 gatatatgaa gaagttgatc gtagagctgg ctcctctctc aaaactagat taaccaattt
      661 acgtaaccgt atggctggcg aaggtgttcc aaaatccaag cgagataaga caaataagtt
      721 agatgtaatc gaaagcgata aaaggttaaa agaaatttat ctatctgtag tcaaggattt
      781 cgctattaag tatggagttt ggaaagaaaa ataaaattcg ataggaggca agtcatgaat
      841 attctaagcg aagagtttct gactcgtttg agaattgcaa ttgttgaagt tgtgaaggac
      901 gcacttaatc agctttcaaa aaagaatctt tcagaaacac gatatttgaa aaaaatcgaa
      961 gctagaaagt atgtgggagg tgtaaatgat caaggctttg agaagctagt agatcatggg
     1021 ttaaaagaaa ttcgtataga tggcttttta aggtatgaca aaaaagacat cgatgaactg
     1081 atggctaaat acaaaatcta aaaggaggac atacatgaca gagatcacca tcacagacgg
     1141 acatatgtct cataccgtga aaacaaaaga agaggcacaa cgtattattg atcgatattt
     1201 tccggaaggg gagtcaacta tgcaaataag aaaaccaatc gttgagcgtt cgagtagcaa
     1261 aatgctactg gcatcaattg ccgaagacaa agaacccgtg tacaaagttc gtcagttaca
     1321 agcactttgc tatatcagct tagttctaaa cgtcatttta ttgtctgttg tctttgccgt
     1381 tttataaaaa aaggaggtta gaaaattgtc gtacaacaaa tggacaagag aagaggagca
     1441 acttcttatt caaaatgtca gatacgacca tcgcggattt gtttgtaatt gtaatgaatt
     1501 agctgagtta cttgggatag ataaaaagcg tatttggccc aaaatccatc aaatgtgtaa
     1561 aaaagatttg atcgatgaag tttattggga tgatccaatc gaaccgccta acaaacgtta
     1621 cagtcagcaa gaagataaaa aaataatttc tatgtatcag tcaggttgtc cagtaacaat
     1681 aatagcgcag gaattgaata gaactgagaa cgcgattcgc tgtcgtctta atatcatgaa
     1741 gaaaaatgga caattagaaa gcaatcgaaa acgcaggtac acaaaaagag aagttgagtt
     1801 attaatatca gaaattaaat tcgataaaaa tggttacgta ttaaatattg attatttaac
     1861 tagactactg catagaccaa agcacgagct tttcagaaaa atttgtctat taaggaaaca
     1921 aggagaaata actataaaac ccgatcgtac aaaggctagt cagaactggt atgacgtaat
     1981 gaaaaaacaa attgataatc atcacaaact gatagctgct cgatatagct atcaaaaaaa
     2041 gacacgatcg ccggcaagca aaatcgtgtc ctgaaaaata ataattacaa ggagagttta
     2101 tcacaatgaa tgaaaaaatc caaaatttaa ttaaagagtt agtagctgaa tgtgcaaatg
     2161 atgatctagg aatgtcagtt agtgtagtcg atgaaaaagg ggaagtcgtt cttggacaag
     2221 ctggaaacga tagcttggtt gcgtggagtg tttaccgaca gtatgagaaa acaaaacgtg
     2281 atttgcagaa taataattgc aattgtgaaa ctcatagtac tttaaaacaa ttgtttggta
     2341 ttgaatatga agatccagag tttgaggaat ggtatcagga ctttctacgg tttgccgaaa
     2401 aaatggatcg caagaaaggt ggtatcaaat tctgatgatc tcagttaaag ggctaggaga
     2461 tgaaatgttc gaagcaatga tgcacaaagc acaacaagat gtacaagaca aaatcttaac
     2521 tgcagcaaag tatggacaaa caagttgcac tgttcgttca aaaggtctga caccatcatt
     2581 tctagcagca ttagaaagcg aaggtatttc aaacatccaa cgtgaagaag gaagtgtcaa
     2641 attattttgg gaattttagg aggcaaatga tgagtgtaaa ggatgtagct aaaaagcaaa
     2701 ttttaaaaat ggaacatatc attgatcaaa tcaacgaggc ggaagattta cttatcagct
     2761 tgaaatcacc agcactgaat aatcttaatg agcttacgac tgatatttcc atcggtgtgc
     2821 ctactcaatt tttagggaga ggttttttcc aagaagaaca aagcaggtgg cataaggtcc
     2881 aacttgaacg tgatcttggt atcacagaga ttcaagaaga aattcgtaat cttgtttcca
     2941 aatcagttca aaaaaggatt gaagcacttg aaagcgagtt gagagcaatg atttattatc
     3001 gcggtgatca cgatgaatga ttttgattct ttaggcgcca gacagcaacc accaaatgaa
     3061 gctagtcctg tcggagttga ttggcaggga aatccgttgt atccaggtga tacttgctat
     3121 ctaacagaag aaggctatgt accagtagac gacattctag agtatgcgca acaacattat
     3181 ccaaaaattg aattaggagg aatttaggga tggataaaag tatacgtact gattcagata
     3241 ttgtgaatca tattaaacgg ttaaacttag atggctttag cgctggccgt tctcttgttg
     3301 ggaatccagc tactatcgct gatgaaaaaa caataggtct atttaaggaa ataaaaaagg
     3361 ttgtaaaaga aaatggggca agttatgacc aaacgagaaa ggccctcatt atggtagatg
     3421 aaagttttta tcaaaactta ttgaaaaaag agaatccatt taaagaaaaa caagctgaac
     3481 caattaatga acctaaaaag gaagatatca acgtaacaag ggaagattta gaaagtgccg
     3541 agttaacaat tgatcctgat ttacctggaa aactagaaaa agaattgcgg gaactaagca
     3601 aagagttttt caataaaatt ctggcatcaa aaaaagaact tctcccctca atgaaagaag
     3661 aagaactggt ttttttacac aaaaaattaa atgaatttag aagagaaact ccatccgatg
     3721 gattcgatgc aaattttcat atcggacaaa ttctgacaga tgtgattctg attgtttttg
     3781 caggtgatga tcttaaatac gctgattaag cttatcgaga ctgtcgtaaa ttgacgttaa
     3841 tctcaactga ttttctatag ttggttcttt gatgaatata tccaaatatt cgacatacgt
     3901 actcagttta gtgaaaaaag cgtctatatc ataaaatttt aaggtttccc aattactgag
     3961 caactgattt cctaaagagt tatagctttt aagaacagat gagtataaat aatattcttc
     4021 atcactaata cgaggatcga ataagtccat tttactacct tcctctaaat ctttaatgtt
     4081 ctgcattact gagcgacata ttccagaaag ctcattatag ttgtcttttt tcaatttata
     4141 catttttatc gcctcacttt caaaataagt atagcaaaaa taaaggagaa taaaatatgg
     4201 caaatgactt aacacaaaca acacaacgtt cattagatga acaggtaatc agtaatcttg
     4261 gacggttaca ggaacaaggc ttagaaatgc caccaggata tagtccgcaa aacgcgttga
     4321 agagtgcttt tttcgaactc actaacaata caggtggaaa tttattacag atggctgcaa
     4381 acaatcaaga aatgaaaacc tccatttcta atgcactttt agacatggtt attcaagggt
     4441 tatctccagc aaaaaaacaa tgctatttca ttaaatacgg aaacaaggtt cagctaatgc
     4501 gttcatattt tggaacaatg gcggtccttg atcgtgtaac tggaggggca gatattacgc
     4561 ctgtagtcgt tcgacaaggt gatgagtttg aagttgcaat ggatggaccg aacatggttg
     4621 tcaaaaagca tgaaacaaaa ttcgagaatt tagacaatga aatcattgca gcctatgtag
     4681 ttatcaagtt agcgaatggt aaagagacca caacagtcat gacgaaaaag caaatcgatc
     4741 agagttgggc gaagtcaaaa atgaaaggtt ctggaccaca aaaggaattt ccagaagaaa
     4801 tggctaaacg gacagtaatc aatcgtgcag ctaaaacatt gatcaacaca agcaacgaca
     4861 acgatttatt agttcaagct gcaaaagata cgttagaaaa tgaatttgac aatgatcgga
     4921 aagatgtaac gccacaaaca gaaaaagtgg ctactctcga acaaaaattc ttttcaaaca
     4981 aaaagattac tgaaccaata caaaaagaac cagatccgat tgtgattcca gatgatatcc
     5041 aagatgaagt aactcgagtt gctgacgtac ctggccattc agaaatagaa caagcacatt
     5101 taatcgaaaa tgaggatacc gatccgattc aagaagaatt attggatatt ccggactttg
     5161 gacgtgagga aggtgtagac gatgtctcag aatttgaaga cgatgagtac cctttctgat
     5221 gaaaattatt actccaatga agctgactgg cagtacatgt cgacatcaca atataagtcc
     5281 tttttaaaat gtgaagccgc agccttagca aagttgaaag gcgattggtc accgacgtcg
     5341 gatcccaaag ccttactcgt cggaaactac gtacattctt attttgaatc aaaagaagta
     5401 catgaagcgt tcaaagaaga aaataaatcc aaaatgtttt ctagtcggaa gccctatgga
     5461 ttgttaaaag atttccagat tgccgaacaa atgattgaac gacttaagca agaagaagca
     5521 ttcatgaata tctatcaagg tgagaaagaa acgattgtga ctggggaact ttttggaacg
     5581 gcgtggaaag gcaaaattga ttgtctgaat gtagaggaag agtattttgt tgacatcaaa
     5641 acaaccaaag acatgcacga gcggaaatgg aatgaaaact acggatcaag agaaacgttc
     5701 attgtcaatt ttggttacgt acttcaaatg gcaatttacc aggaactgct ttaccaacaa
     5761 tacggaaaaa gttttatgcc gatcatcgct gcagtgtcca agcagacacc aagtgaagca
     5821 agactgatca cgattgacca ggataatatg gattatgagc tggttatgtt aaaagaaaaa
     5881 atcgaaagaa ttgtcagagt gaagaacgga gaagagaagc cgaaccattg cggtttatgc
     5941 gagtactgta ggggtaatct tccaataact ggattcacta gcatggacga tttatagaac
     6001 ggtggtgatt atatggctga aggatgggtc aagcttcacc gttctataac tgaaaattgg
     6061 atatgggaca atccgcaata tctaaagtgg tggcttgatt taattctaat ggccaaccac
     6121 aaagaaaaga aaattctttt taatggttca tttaaaaaag ttgatgttgg gcaaagaatt
     6181 acttcagaac aaaaattggc tgaacgttgg ggagtaagta gaaatactgt cagaaaattt
     6241 ttaagtctac ttgttgaaga tgacatgata agcattgaaa aaagcaggaa aaatggaaca
     6301 acgtataaag tcaataacta cgccatttat caagggtttt cggaagaaaa aaaacaacag
     6361 actgaacaac gagctgaaca tcaaaaggac aacgaactga acataaacaa gaatgaaaag
     6421 aatgagaaga atgataagaa taataataat cttcgcaact ctcgaaaaac acgagagtat
     6481 gcagatgacg atccaaataa aaaattggcc attcttttat taaaactcat tcgaaaaaat
     6541 caaaacatca aagaacctga tttggataaa tgggcgaata cgattcgttt aacaattgaa
     6601 tctgacaaac gaactggtag agaagttcaa gacatgattg tttgggccac aagtaatgat
     6661 ttctggtcag gcgtaatctt atctccaacg agtttgagaa aacatttcga taaaatggct
     6721 atccaaaaaa ataaaagaac gcaacaaaat atttctaatg atgagttacc agaaacaggt
     6781 gaggattggt aatgaataaa aaactaagtg caatggctgc accttacggc ggattaaaaa
     6841 cagcagatca taattgcccg aaatgtggtg atccattgta tatctggaaa acaaaaaata
     6901 aagatggtac tgatcgatgc ggtcctacat gtatcaataa gatttgtggt taccgagaaa
     6961 tggtaactaa aaatcaaaaa gaagctatcc aaaaagcgaa tgaagcaatg aaaaaggacg
     7021 ctatcaatcg aatgcttaac agttcaatga ttacagatga tgccatatgg accttcgatt
     7081 ttgatggata caaagtagtt gatcaggaaa cagcacaaat aaaagcgatg gctcaagaat
     7141 gggctaaaaa aatcgtaagt ggcagcacga ttcacgcggt tattactggt agaacaggag
     7201 ccggaaaaac ccatctaggt gctgcagtga tcaaagaagt aatgatggca tctaattata
     7261 aaattgcctg ttcatttata agctatcgag aattattaga gcaattgaaa tttgcaatga
     7321 atgatccaga agcaagaaaa gctgtgacag gttcgttgat ggctgaaatt aaaaagacgg
     7381 attttgtagt gattgatgat ttaggtgcgg agctaggaag aatggaagaa aacaatcagg
     7441 caacgcctta tgatgttgat gttctcacat cgctcacaga agctcgtcta aacaaagcta
     7501 cgatattcac aaccaattta tcatcgaagc aattaaaaca cgcatacggc gagcgagtgt
     7561 tctctcgtgt aatgaatggg acaaaaggaa acatagccgt attcaaaaca acgacagaca
     7621 aaaggaggaa tccagtttga cctttgtagt gaaaaaaatg tgctacttag atagccgagg
     7681 gcgaggagaa gccagtgtcg agtttgctaa acaccacacg actaaagaag aggctgacct
     7741 tgttgcaagt gtttgtggcg gcgaagtagt cgaagttatt aaacccaaaa gacgattttc
     7801 aaaacctaag acaaagccag tgaaaaaaga gtgttgttgt ccgaaaagta accaagcatg
     7861 gatgagaggt gcaaaatgac ttgtttaaaa tgcaacgatg aaacagttat ttggtataag
     7921 acatcgcttg gatggtcaac ttgtgaacct tgtccaatat gcaatgaaaa tggacgacgt
     7981 tcgaaagaac gactcgaaag actaaagaag gagtatagca aatggcaaca agaagcaaat
     8041 acggaaacaa aaagcacgaa gtagacggca tcacttttga ttctaaagca gaagctcgtt
     8101 attacatgaa gttaaaacga aacggtatga gttttatgcc tttatctgaa acctactgtg
     8161 ccatgcaaga aaatgttctg ctgcaagaag ggtatctatg caacgatcgt aagattgcac
     8221 cgatttatta ccgagctgat tttgtgattt atgagaatgg ccaagtgaaa aaagtgattg
     8281 atgtcaaagg ttatcaagat gcgatctcta tgctgaaaat gaaaatgttt gctcatcgat
     8341 acggctttcc agtgacattt gctaagttcg attcaaaaat caataaattc attgaaatgg
     8401 attgttttga atcagcaaga cagcagcgga aaagacaaac ggaacgaaga aaaaagaaat
     8461 taatggaggg aaaataaaat gacaaaacag gtgaatttca gaccagaatt aaagaaagta
     8521 acatcaaaat caaatgggaa cacagaagta ttactagttg ttagcaacgg atcattgaga
     8581 ggtagtactg aaaatttaac tgaatttctt ggctcaacag tgactgtagt aatccagcca
     8641 gaaactatcg aatacacagt gccagtaaat aaacaaacga aaaaaccgaa tgtcgaatac
     8701 gttgtaaatg ctgatggcac aatcgacatg cttaaagaag agcagacttc acttgatgta
     8761 ggtgatggag tagaagaagt tgaaaatgtg aaaatcctag tatcaaagga aacgattgat
     8821 gaatttatta aaacagcaac gacattgcaa ctaccagaaa acgtaactgt aaatattcga
     8881 gacgttctta tccgtttagc agaaggcgat agtatgagtg aaattgctgc agatcatgaa
     8941 ttatcagaga ttgctttaat agatcaaatc gaattagcta gacattactt tgctccatat
     9001 gcagatagct ggtccaaaca taaggacgac atcatttttc cagaggaaca atgagagcaa
     9061 ctgatccagt aattatcctt gaggaagcta aatttatttg gactcacgaa gagatagagc
     9121 aagcacgctt gctcttttct caaggagtta agccgagcaa agtagctgaa ataatggatc
     9181 aaaagattct tgatgtcgga ttgcttttgt tacatctagc agaaaaaaat ttgatttgag
     9241 gtgaaaatga tgattcaact tgcaggcata caaacaggga aaatttattt ttccggagaa
     9301 agcaaaagtg aagctagtca atggttgctt aaaacatata cgaacaataa gaagctgaga
     9361 aaaaaatgtc ctgatctatt tttgaaggat gatcagatta tgccagaacc gatgattttg
     9421 acaagcaagg agggcggcaa atgcgaatct tagagattgt ttgggaagta attaaattta
     9481 tttccaatat gtattttacg ttttggttag tgtgctgcct tctatatgga ttcaaatggc
     9541 aagtagggaa tttcatgagc gttaaattgc cggggatatt gagacggagt aaaagaattt
     9601 acaaggagga cagcaaatga taccgagatt tcgagcgtat tcaaaagaag aaaatgaaat
     9661 gtactatccg cataatgata aaaatgtgga ttggacaata gacgatgaaa caggctttat
     9721 tgctccactt gtaaatttag gcggcggcat gtggggaatg atagacaaat acgaacttat
     9781 gcaatcaaca ggactgaaag ataagaatgg tgtggatgtc taccaaggcg atatcatcag
     9841 atgtactaga ggttgtcatc atgaagtaat ttggttagag gaatatggag gaatattttt
     9901 tggaggaatg ccggcgtggt atctttcagg attaagtaat ggctatgcgt ggactggtgg
     9961 agaagaagtc atcggaaata tatacgagaa tagcgagtta ttggaggaaa aataatgaaa
    10021 ctaaaagacg gattttacgc tagtagccac ggtatcggcg gtttaatgct agacatgccg
    10081 acaaagaatc ctaaaacacg tgagaaacca aaagtcaaag tcggtgatat ggttcgctgt
    10141 gaagcagaag ggttcatcta tccgtttcgt ggatatgtag agcatctcta taatcactca
    10201 gcaatcattc gtattgaaaa cacgatgaaa tgtgacaagt ggttagcgaa aagtaaagag
    10261 aatttagcag tggtgagatt ggtggatatg gaggttataa acaatgaaat ttaaaatcct
    10321 tgaagaggac acacgctatg aattagaaga aaaattaaat gaatttgcaa agaataatga
    10381 aattcagcat atatctttaa cggcttttat ggccggttat acagcttact atgcagctgt
    10441 tgtgagctac gtaagtcaat aaagggaact cgacaaataa aaaaagccgg atcgctccga
    10501 ctcaaaataa ataaacacga cacgtttatt atatcacata aaaggagcgg tttgacttga
    10561 tgcaattgtt acgagaggta gatttcaaac agacaagatg taatgcgaga gatgtgctga
    10621 agaactttcg gcgtttggag cggatggcag gtcgctcttt gatagatatt aagtcgccta
    10681 tcataaccga tatgccgaag gctccgaaat atggaaacaa ggcagaagac gcgatcatac
    10741 agatgatgga tatagaagcg gagagagacg cgattttagc ggctttgatg gctctcagtc
    10801 tgattagtcg tcagatactc tactacagct tctgtgacgt aaataagcac tctaattatg
    10861 aaatagggca attgatacga ggatacggag agaagaatgt agagaagctg aaatccatcg
    10921 cattgataga atttgcagaa gcatacaaaa aaggcgtgtt agttcagtat cgttgatttt
    10981 gtagggtttt tgtagggata gtgtagggtt tttgagtgtt ttaacgtgat attatgatag
    11041 tgtcgaaaga ttagtgatag gtctgagaca aaataataat aaaaggaaca tcgttttatt
    11101 attgtttcac aattaagctt cgatagacag ca
//
LOCUS       DAGCYL010000086        11130 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.087, whole genome shotgun sequence.
ACCESSION   DAGCYL010000086 DAGCYL010000000
VERSION     DAGCYL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.087"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            92..1351
                     /gene="lysA"
                     /locus_tag="MB216_001698"
     CDS             92..1351
                     /gene="lysA"
                     /locus_tag="MB216_001698"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBT4526536.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1338..2138
                     /gene="dapF"
                     /locus_tag="MB216_001699"
     CDS             1338..2138
                     /gene="dapF"
                     /locus_tag="MB216_001699"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBT4526537.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2141..3022
                     /locus_tag="MB216_001700"
     CDS             2141..3022
                     /locus_tag="MB216_001700"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBT4526538.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3019..3735
                     /locus_tag="MB216_001701"
     CDS             3019..3735
                     /locus_tag="MB216_001701"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBT4526539.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3741..4436
                     /gene="dapD"
                     /locus_tag="MB216_001702"
     CDS             3741..4436
                     /gene="dapD"
                     /locus_tag="MB216_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBT4526540.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4452..5453
                     /locus_tag="MB216_001703"
     CDS             4452..5453
                     /locus_tag="MB216_001703"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBT4526541.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5450..6811
                     /locus_tag="MB216_001704"
     CDS             5450..6811
                     /locus_tag="MB216_001704"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBT4526542.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6771..7922
                     /locus_tag="MB216_001705"
     CDS             6771..7922
                     /locus_tag="MB216_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBT4526543.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7897..9066
                     /locus_tag="MB216_001706"
     CDS             7897..9066
                     /locus_tag="MB216_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBT4526544.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9241..10620
                     /locus_tag="MB216_001707"
     CDS             9241..10620
                     /locus_tag="MB216_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBT4526545.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10901..11130
                     /locus_tag="MB216_001708"
                     /pseudo
     CDS             10901..11130
                     /locus_tag="MB216_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3704 a         1945 c         2493 g         2988 t
ORIGIN      
        1 attttgttta atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa
       61 acggaaaaaa agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac
      121 agcaagcaca ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat
      181 gagacaaacg atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt
      241 gctttacgcc tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg
      301 attcgggcta gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc
      361 tagagatact atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact
      421 tgaaaatgat ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga
      481 attaagccaa cgctatttaa aaaaaatctg tatcatgctt cggttgaatg tcggagtaga
      541 agcacatact catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta
      601 tgaaagtaaa gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt
      661 agaaggattt catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga
      721 aatcgacaaa ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg
      781 cggagggttt gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact
      841 aaaaaaactg attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca
      901 attactgatc gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat
      961 tggtttcatc aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga
     1021 taatatccgt ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc
     1081 ggaagaaaaa acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt
     1141 aattaaagaa gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac
     1201 gggagcttat ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt
     1261 ttttgtcaat aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact
     1321 aaggggggaa gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct
     1381 tattagatta cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag
     1441 aaaaatatga tatagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt
     1501 acaacaaaga tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat
     1561 acgtaaatga tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca
     1621 caagaaaaat tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc
     1681 cagatgattc tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa
     1741 tagagatcaa tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcataccg
     1801 ttgtttttgt tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc
     1861 atcatccttt gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag
     1921 aattgatcgt ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt
     1981 gctgtgcctc ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg
     2041 ttcacttgga acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc
     2101 cagcagttca tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt
     2161 ggcgctgatc acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt
     2221 gatccaattc catttagatg aacaaacaga tggattatta gttttaggta caacaggaga
     2281 atcttctacg ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt
     2341 ggctggccgt gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga
     2401 aaaagcgaag cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta
     2461 caacaaaaca agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc
     2521 gattccgctt attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt
     2581 attagtaaat ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat
     2641 ggcttatgtg atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa
     2701 tgatgatttg atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc
     2761 gaatatccaa cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca
     2821 gcaagcaaaa gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac
     2881 caatcctatt cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg
     2941 cctgccttta gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa
     3001 agaaaggacg gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga
     3061 gtcgaagaag ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa
     3121 gaagcaacta cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca
     3181 gataatttag agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc
     3241 acgacaggtt atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct
     3301 gtgatgtata gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa
     3361 gcagctgcaa tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa
     3421 aaagatgcac catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag
     3481 ctgcagcccg tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac
     3541 agtattcgtg caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa
     3601 atatttacga tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa
     3661 gcagcggact ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg
     3721 gacaaagggg tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat
     3781 ctaaaaagac gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa
     3841 gtaaagtttt tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc
     3901 cggttttaga agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat
     3961 acagcgcagt tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta
     4021 ttatcagaga tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca
     4081 atattggtgc agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac
     4141 gagcgacagt gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg
     4201 aaccagcatc ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag
     4261 tgattgttga aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt
     4321 tagaagatgt ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa
     4381 gtgatgatca gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga
     4441 gatggagaaa aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc
     4501 aaacgatgct caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag
     4561 cttcaaaacg ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg
     4621 aattgacaga aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg
     4681 tatccaaaga gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct
     4741 cagcatggcg ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact
     4801 atacattgaa tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc
     4861 ttaaagcttt acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag
     4921 tttctggttc cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac
     4981 cagttttgta tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg
     5041 aaaatgggta tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc
     5101 agcctgcttt gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag
     5161 tctctgtggc agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca
     5221 gccagtttcc agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca
     5281 ttcttgctag aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag
     5341 agaacggatt gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg
     5401 ccgtacagat tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac
     5461 gctgaaagta gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt
     5521 ccgtacaatt gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa
     5581 acgtacgtca gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca
     5641 gcatcatgtt gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag
     5701 agatgttctt gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact
     5761 ggaagacgaa aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca
     5821 actgatggct gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga
     5881 ctatgatggg aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag
     5941 atatccgaaa atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt
     6001 attgggccgt gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga
     6061 aagttatgag aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga
     6121 tcatcctaaa atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg
     6181 ggctaacgtt cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca
     6241 aataaaaaat accaatgatc ctttcgctca agggacaaaa atatcaaatc acgaaataga
     6301 aaaggaaaat ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt
     6361 caaacgtcat atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa
     6421 acatgagata tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc
     6481 agcagaagcg attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg
     6541 cgtggaagaa atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca
     6601 taaagaattg attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg
     6661 cacatcgatc ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa
     6721 tcaatatgaa acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag
     6781 aatatcgaat ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa
     6841 aatatttata tgaacaagta aaaaacagcg aggccgttat ccatgaaatc ggcgaaacag
     6901 gactactcct ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg
     6961 atgcactgcc agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata
     7021 tgcatgcatg tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa
     7081 ctaaacacat tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa
     7141 tttccggtgg cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag
     7201 caatatttgg ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag
     7261 gacctttgat ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata
     7321 ttgcttctag tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg
     7381 tttatgattt tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa
     7441 tccaaggtgg aacgatccga aatgttttag ctggagaagt tattgtatcc ggaagtatcc
     7501 gttcttacag ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac
     7561 agtttcactc tacagctact atttcttttc gctataatga cggctatcca gctgtaatca
     7621 accatagagg cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc
     7681 cagttcttca agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct
     7741 ttttaggtgt cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg
     7801 ttttagaaaa tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa
     7861 aaatcgatag cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt
     7921 agtcctaggc tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg
     7981 tcgataccag atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat
     8041 gtcaagcaag caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga
     8101 ggattagagg aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact
     8161 tatgatccgc aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg
     8221 ttgttatctg ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga
     8281 tatcagccat tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca
     8341 gggtttgtct gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc
     8401 aaagcagtca ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa
     8461 ttgaaagcaa tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta
     8521 tatgctgaat tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa
     8581 acaatcgtga taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt
     8641 atttttgctc aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact
     8701 gccgctacaa cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat
     8761 agcgagaata tgaaagagca atatcaaaca cgaagagact atctggtcca gcaattggcg
     8821 cctttaggat ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca
     8881 gctgccattc aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt
     8941 gcgtgtatcc caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat
     9001 gctagcagca tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa
     9061 cagtgattca tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg
     9121 agacgattgt tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg
     9181 taatgttaag tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca
     9241 atggaaaaaa tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg
     9301 gaactattga cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat
     9361 atttccttca tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat
     9421 gatgtacgta atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat
     9481 cacgaagtaa aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat
     9541 aacgaaacaa aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac
     9601 aatctgccag taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg
     9661 gcaaccaaaa tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt
     9721 gcgggttaca ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta
     9781 ttgcttgatg tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt
     9841 ttagaacaac acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct
     9901 tttactggta acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat
     9961 ctagtcattc aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac
    10021 ttagatgaac gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg
    10081 tatgcagtag gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc
    10141 gctttagcga ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa
    10201 gtaccaaaca aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat
    10261 cattttgcag caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga
    10321 gtgtcttatt atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca
    10381 aaagtgtcca tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta
    10441 ttatctacct atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa
    10501 ctgactttag aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa
    10561 tggagcatgt tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa
    10621 gaaaacgaaa tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag taataagaac
    10681 agccaagtaa aaacagcttc ttatgttttt aacttggctg gacttattac cgcagatgca
    10741 agcttttgaa caccgtttat tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc
    10801 ccaaaagtta gaccaaaaaa tctaactttt ggaggtcggt attttttatg gttaaatata
    10861 cttttgaact taaattaaag atcgttcatg attatttgga tggcaaaggc gattggaccg
    10921 taaaaatcca gatcaagaga ttgaagcgga aatgcttaaa attcgggaga agcacaagga
    10981 ttatggctgt ttacggatga ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa
    11041 gaaagttcag cgcttgatca agaagctagg aataaaagta acggcataca cgcgtaaatc
    11101 acgtcgctac aattcttatc gaggaaaagt
//
LOCUS       DAGCYL010000087        11074 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.088, whole genome shotgun sequence.
ACCESSION   DAGCYL010000087 DAGCYL010000000
VERSION     DAGCYL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.088"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            41..910
                     /locus_tag="MB216_001709"
     CDS             41..910
                     /locus_tag="MB216_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBT4526546.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            930..1277
                     /locus_tag="MB216_001710"
     CDS             930..1277
                     /locus_tag="MB216_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526547.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            1290..2552
                     /locus_tag="MB216_001711"
     CDS             1290..2552
                     /locus_tag="MB216_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526548.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2632..4008
                     /locus_tag="MB216_001712"
     CDS             2632..4008
                     /locus_tag="MB216_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBT4526549.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            4005..4634
                     /locus_tag="MB216_001713"
     CDS             4005..4634
                     /locus_tag="MB216_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526550.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            4653..5132
                     /locus_tag="MB216_001714"
     CDS             4653..5132
                     /locus_tag="MB216_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526551.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            5634..5687
                     /locus_tag="MB216_001715"
                     /pseudo
     CDS             5634..5687
                     /locus_tag="MB216_001715"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5691..6290
                     /locus_tag="MB216_001716"
     CDS             5691..6290
                     /locus_tag="MB216_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBT4526552.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            6306..6782
                     /locus_tag="MB216_001717"
     CDS             6306..6782
                     /locus_tag="MB216_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526553.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            6799..7701
                     /locus_tag="MB216_001718"
     CDS             6799..7701
                     /locus_tag="MB216_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526554.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            7774..7965
                     /locus_tag="MB216_001719"
     CDS             7774..7965
                     /locus_tag="MB216_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526555.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            8129..8716
                     /locus_tag="MB216_001720"
     CDS             8129..8716
                     /locus_tag="MB216_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526556.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            8716..9615
                     /locus_tag="MB216_001721"
     CDS             8716..9615
                     /locus_tag="MB216_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBT4526557.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            9615..10529
                     /locus_tag="MB216_001722"
     CDS             9615..10529
                     /locus_tag="MB216_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526558.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            10581..10796
                     /locus_tag="MB216_001723"
     CDS             10581..10796
                     /locus_tag="MB216_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526559.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         4248 a         1498 c         2255 g         3073 t
ORIGIN      
        1 atttatctta ttatgataac acaataaaag gaatggtggt atgaaaaaga agaagtttag
       61 ccgatcgttg ttaggaattt tggttttagt tgcggttggt cttagtggca agggttactt
      121 tcagaataac agtactcagg aacatgattt tcagatcaaa agtagtgttc aggtaggtaa
      181 taaagcagac agtgaatcaa aagaattttt agggatacgt tatgatccaa acaaatatcc
      241 aaataatatc gtaaacatta atgatgggaa atatacactg acaaaagaag accatgaagc
      301 attatcagaa aaaggcagtc aaaccttttg ggtaaattat gcagatttag atcaacttgg
      361 aagagcagga gatgttgaag cgttgatcac ctatgatgct gtaaattctc actcatctaa
      421 aaatgtggaa cgtccatctt tcaaaagttc tgtccatatt gcaggagaat acaaggatgg
      481 agtatttgat caagagagag atacttgggt cggcaaagat tcaaacaatg aaatcactca
      541 gctagaaggc tataaaggtt atctctataa caaaagtcat tcattagcat ggtcgctagg
      601 aggagatatg gaggtacaca atttaacact tggaacgaga gcgcaaaatg ttgggaaaaa
      661 tgatcagaat ggagggatgg cttactcaga aacgattgta cgtgatgcaa tctataaaaa
      721 taagaatgaa aaagtattgt ataaagttga tccaatttat aaagggaatg aattagttcc
      781 tcgtggttct catgtcgaag ctttctcagt taacgataat ggaaaaacgg taaaccttaa
      841 tgtttatgta tttaacaatc aaaaaggaat caacatcaac tatcaaaatg gtacttgggt
      901 aagagattag aaattgagaa ggtgaagaat tggaaacaat aagcatcaat ggattagata
      961 aatcagttgc aaatcgaata aaaaaaggaa atggctacta tcttctgaag aagggtaaat
     1021 attcagttgc tagagagtta gaaacaatga gtatcgtgta tgtagctaag aatcattttg
     1081 acgtagaaca ggctattttt gatccagatt ctgaaatagg gaaatgtgtt gaattatcag
     1141 agaaaactgt tagtacaaaa ggggcgatga tcattgagag agaaatgtta gacgaaatca
     1201 gagtaatcat tcctaaagat acgcagatgt gtgaggacag cataagtgac gatgaaccaa
     1261 aatcagaaag aaaataagga gatattcaga tgagcgaaaa atccattgaa ttaaaggaat
     1321 ataagcgaag cgatggagaa aaatattatc ctgtagccag tttttctaaa tcagtatatc
     1381 tggttggacg tgcattggta gttggtgcaa ttggcatata tattttgcat tgggtaacaa
     1441 aggttgtaca actgcttgta gataccttag taagtattct tgtctcgtta aatgatgtta
     1501 acggaacaga tgatttagga ttaaaagaga tgtttaaaga gttactcaat gcgttttttc
     1561 ccaattattt aagttattca tataatgtac aaagcttttt tgcaaagcta cacttggtag
     1621 acatctctat tttagtatta gaagcaggaa cggtattctt tgttggatat ttggttactt
     1681 tgatgttcag gcatcactat ggagaacaaa gaccattttt tgatgatatg gaaagtagaa
     1741 ggttgaaaag agaagctcta gaagctatgg atgtgaagta tgatccaaac aagattatag
     1801 gagatgaaac gaacaacaaa aaaagaaaac gaaacaaaac gcaattagaa tccgatgtgc
     1861 tatcaacgct taaaaatatc aaaatttctg ttcatacaag acgtgatcta agtgtcaatg
     1921 aagatattcg agaatatatc gtaaaaatcc cgcaaccaaa agggaagaaa gttagagaaa
     1981 cagttttacg acaaattcaa gatttagggt cgatcctgac aagagcaaca agcggacgag
     2041 ccaaatttgg tgaaaaagaa gatgaagttt caggcgatta tttcattttc cgagcatcag
     2101 taaatattga aagagaacgt aaggaaagag taagtaccaa aaacaaagaa acggaaagag
     2161 caacatcttc aatcgcagtt tcaaattcac aattttcttt tccaatcaat ctctttgccg
     2221 ataattccaa aaatatccaa aaccaaaccg aaaaagctag aaaatatgcc gaaactcaaa
     2281 agcaatcttt agaactttat ctatcttctg ctgacgtacc tgcaagtcta acagatatgc
     2341 acgttgggaa tacatctatt gaatatttgt ttactttatc tcgtaccaga aaaattcaac
     2401 taagctcaat gaaagaagaa attgaaactt acttgcatat atcaggtgta atcgtgcgga
     2461 tcaacgcagg gcgtattcaa attactttac cattaggaga aaatgatcgt attccaattg
     2521 atgtaccatc aatgatcaat agcgtgtttt agaagacatc acaaatagaa acagaaaata
     2581 caaactacca gttcacaaac agaaaagtac cgagaaaagt gaggaataga aatggctatg
     2641 aaattattag agaaagtcaa aaagagagcc gaaaaagctg aatctatttt gctagataaa
     2701 gaacctaata taaagcttaa tggaaaattc agtgtcaata tgttgaacac aaaggctaaa
     2761 gacccaacag atgctatttt tggaattaag cctgatggca caattgctca tttttgcatc
     2821 agcgaaatgc cacacatgtt ggttgcagga acgacaggat cagggaaatc agtattactg
     2881 aatgaaattc ttgttacagt aatgtgtcac tcaacgcctg atgaattaaa aataggaatt
     2941 gtagacccaa aaagggttga atttggaaga tacaaaaaat tgccgttcat gttggctgat
     3001 ccaattacag atatggatga agcttatgat ttttttgaat atcttgtgat cttgatgcat
     3061 gaacgttaca aactgatgga aaaagcaggg ttacaaaaca tcacgctata taacgaatat
     3121 gccgaaaaaa atggattaga tagatttccc tatgtgatct tattagttga tgaatattca
     3181 cagttggttg gaacacataa agaagtagaa ggtttgattg ttcaattagg acaaatggca
     3241 agggcagcag gtattcatgt tatcttagca acacaatctc ctcgatcaac agttgtcact
     3301 ggtattatca aagccaattt tccgtcacgt gtttgcctta tggtagcaag tgatttagaa
     3361 agtaggattg tattagatga aggcggtgga gaaagtctaa gtccaaaggg agatatgctt
     3421 attaagcttg ttgatggaag tatggttaga gcgcagggag cttacatcag taacaaagaa
     3481 attgaaacaa tcttcaatca ccttagaaat actatgccag aaccagaata tgttgattac
     3541 cgagcaatcg tagctgaaac tcgtggtgag ggcggtgaag acgaggattt tggtggaatt
     3601 ggtaaagctc ctgtaagaaa atctgctaaa agaaatttga acacaccttt agcaaatcca
     3661 tcatctaaag agccatcaat tgaaaagaag agtgcgtttg ctattaagct tgaaaagaac
     3721 aaagaagaaa aagtcgagca gggagctaaa acactagaaa aactgcaagc agaaaaacta
     3781 gaaaaagttt taagggcatt aggaaaagct gatcctgaac cgcaaacctc aaacaacacg
     3841 aataataaac agccgattgc atctaaaacc aaacctaaag ctaatgatcc aaaaggctca
     3901 gaaaacgtag tgatcacaac tgataaatct gaaaagaaac aaataaatgg aagtacttca
     3961 cctatcatgc aaaaaatgtt aaaagcaaaa aaacaggtgc ataaatgatc atcgtcagtt
     4021 cttttttaag atggcttact ccatataaat atacagatga agtagttgtg aaacgagcga
     4081 agaaaagaga taaaaaagcg gtactaactc ctaaacagat gcttgatcat ggcattttag
     4141 gggtgaaggt tttagaagtg aataaaccgt ttgaatcaaa tagtgcaaga gaaaagttgc
     4201 ttggagctga agccgatgtc gcatattttt accatgacaa aagcaagcat caaatagcta
     4261 ttaaattcaa tccaaaatcg gcatggcggt atcaagcaag agatgacttg aaacgtagga
     4321 aacttagacc aatgctctat ccagaaggaa aagaagcatt tgatagaacc cacatcgttc
     4381 ccattggttt tcatggtaca gaatcagata accgcctatt agtaggttgg aacagcaatc
     4441 aaaatagagt tcagatcaat aaatttgaga agaaagtaag gaaattcaac gatcgaaaaa
     4501 cgatcctatg gtttgcagat atttgtaaac agccggatgg aacagcaaag tgggaagcgg
     4561 tgatatttga cgaaaatggt aaaaaagtaa tgtctgaaac atggcatgat aaagctaaat
     4621 ttgtttggca gtagcagtag aggtgaaaaa gattgctaga actttttgaa gcaaatagaa
     4681 caggctttaa tcaaaagtta gaagtgggag aaactataga atataaagat aaaaactata
     4741 ttattatcgg aatctataac acgagaatta acagaatagg tgatccacgt ataaccagtg
     4801 acttggtttg tcaatctgtt aactattcac ctgatctgac aagcaggtat aagaaatacg
     4861 tgttattaga atatagacac aagattactg atgaaattgg tgtaaacaac acacgaaaca
     4921 tatttaagat cggaacagtg attccttata gtaacaacga agaaaaaata ttttatcaga
     4981 tttacggaat agaaaagttt gaatatgaac atgtcgatct gctagtgacg taccaaatgc
     5041 gtttaataga accttggtca caggcagaaa ttgataaagc agtaaaattg aaccgattat
     5101 caaaatttaa cgtattagga aatgcctttt aaaaatcgca atacacaaac agaaaaatac
     5161 caaaaaatta gcaatgaacg taggtaagaa catgacaata aataataata accctaaaaa
     5221 atataatgct cttgtccaat gctatatcga aaacaaaatt acaatgcaag aagtcgctga
     5281 tgaattaggc aaatcaaaga gcctagtatc acacgatttt aagcacaatg tttttccgaa
     5341 cccgattgag ctgaggaaaa aatttcaaaa gagagtttat caagaaatag gtaagtggat
     5401 tgatgaaggg aatgcaattg accctttaat tgaaaaatat ccttttttaa aaagcaaaaa
     5461 taaaattcaa aggttaattc gtcaaggcga tattcaagct aaagaacctg ttttgacaac
     5521 caatgatttg gctaaaataa atcttcataa acaaattatt gaagactttg aaaaaaatct
     5581 ttcaacaact gaaattttaa aaaaatacaa tattccgaga ggaaagctaa catcgactct
     5641 ttttagagaa tatggagaat cggcagttac taaagcgaag gaagtagcga atgagagaaa
     5701 aaaacaaaaa tagtagtcga tacctagtag cgactgtagt ttttgtttgt gtattgattt
     5761 tcctgtttga ttttttgcag attggaaaaa gcataattgg aaatacattg agtgcaaagg
     5821 aaatggttgc tttaggtggt ttgtatgaag gacacttgtt cccgaatata ttcatttcta
     5881 tcgtttctca tattaacttg taccatttaa tttctaattt attgttctta gtagttcttg
     5941 aagaagcact gaatgatcag tatgagtggt taacattatt gatcgtttta atcatatcag
     6001 gatttctagg aagcttagtt ccttttctgc tacacagcaa catcgtaagt atggggctta
     6061 gtggaggtat ttttggatgg attgctttat ttgcgattgc atacatccgt tttgatagca
     6121 agaaacattt attactaagt gtttttgtat tattgataat cggcatttta ttggtatcta
     6181 cttttgaatc aggcgaggca aatgtcttgt cacatgtaat cggatttgtc tgtggaatga
     6241 tcttgggatt tattctaaag aacgtttcat tgatcaaaca aaagaattaa aagggagtgc
     6301 taaaaatgcc aaaaagaaaa gaaaagaaaa ttccagtgtt tttgtctgtc tcagaaatgt
     6361 tatcacttca aatactgtta agaaaaaaga ttgaaacaat agacgaagaa gttagaaata
     6421 aaagggctat gtacgaacaa cgtgaagcgc taagagaacg aatccactat tttgatgata
     6481 tacacactaa aatctatgaa gcacgtacaa atcacgaaga cgaaagtaaa ttaggattag
     6541 ctcaacaacg gatacttgaa tggcttaata cgaatactga tcaagaaccg ttaagagtga
     6601 ttagtaatct ttttgaattt ggaacgaagc attggtttaa tgctgtagag aaggatgctg
     6661 acttacaaga agcgtttgat acgctaagta aggatagcaa atccatgcga gatttattga
     6721 ttacttatct aaatgagcat gagtttgaac aaacgaaaac caaaaagaaa aataaagaat
     6781 agaaagttgt gatgaatcat gctattaacc ctgttaggtt atttatgttt aattcttgct
     6841 ttttttgggg caatcgctat aattattggt caatttcaaa caaataatcc gattatgcgt
     6901 aacataggca ttggaattgt gattgtaacc tttattgtgt ttaatgccac taagattttt
     6961 ggaagtaacg ataaacaatt tggtatatcg ggagttaata atagttatgc tccagattct
     7021 ttgggaaggg tagtaataga tggaacattt gaaaatgtaa cgagttataa aaatgtttcg
     7081 ctgacagtga aggaagatgg caagaaagca tcagacgacg ttctcgctac tattaagccg
     7141 tctcaatcac aatttgaagt gttttataca gttaaagaga atagccctgt acacaagctg
     7201 acattggttt ttaacgttga tggagaagaa tactataaag atgtaaaaat aaataaaaca
     7261 tcaacgaaaa agattatcga atcagcgaaa aaagaagtag ctacagaaga aagtacaact
     7321 gaatcaagtt ctactgtcga agcggaacta ccagataaaa ttgattttga tcctgaaaaa
     7381 gtcaaatcgt ttgaatctgt ttctggttct atggctgatc ggtttaacac tgaagtcttc
     7441 aaagctatca aaactgttaa attggatact gaaaagcaaa tgattaatgt taccttattc
     7501 aattcagttg aatctatgga tcaggataaa aaagttacgg ttgcaagaga actaggaaaa
     7561 agagtaattg gtttgtatcg tggtactgct aaaccagatt cagacggtgc agatttgcca
     7621 gttcatgtag tttatgaaga cggtaaagtt tttggagatt ctacagcaaa cgaagcatta
     7681 acgattaaat tggctaaata gccagaaagt tataataaac aaatggtgtc acaccaataa
     7741 tatataggtg tgacaccttg gagatgatag ctgatggtag taaataaaga aaaagttaga
     7801 aagtatgtga ctttaaaaat agcttctgtt aaatatttag aaaagcgagc taaagaagaa
     7861 gaaagagcaa gaggtaagaa agtaacagtt tcagagttgt tagaagaatt gatcgagaat
     7921 gatcaaacaa taagacgtgc atttactaat aggtctatca gataacaata taattaataa
     7981 aaaagaaaga gtgatttggt cttagactaa atcactcttt ttatataaag tttctttaca
     8041 ctcattccga ataatgttaa aattctttat atgatatata gttccttgtc agaggaagag
     8101 ataaccagca atattggagg gataataagt ggaaaacaat aatgatatta gacattttag
     8161 cattggggaa agagttacaa cagtaagtga attatcaaag gattatagag ataaagaggg
     8221 aacgattatt tctcttaata agaatcgaag aggcgtttgg caatatgttg ttgatttagg
     8281 tgatggcgtt acagttgagt gtaaagatag tcaactgatc agaaataatg gcagtcattc
     8341 cattgaaaaa gcaattcttg tatacccaaa agtatcaagt aagatcatga atgactggtt
     8401 gcttggaaaa tttggtgagt acaataaaaa agaaatcgca gaagtgatcg gcaaatctta
     8461 tccaacagta ttagggtatc taaaaaagat aacgagtaaa gaagcaaaaa ttgaggatgt
     8521 tttaacagta agagagcttc aatcattaat cgagtatcgg aataaacaaa taacaaatcc
     8581 tgagtacctt agaaaagaag atggtctatt gaaacaattg gaacaagtaa ataaaatccg
     8641 gaagatgcaa aaggtagctc ctttaagcct tgataccttt aaaaaaatct atttgaaaga
     8701 gatcaaaaaa gactaatgtg gactgtaact ttaccgttcc aacctgtatc tgcaccaaga
     8761 ccaagtgcaa gaaggaatgc tgctgatgaa actaatgacg tagcagagaa aaggatttca
     8821 acctactatg ataaaaagta tcttgattat ttgaaaaaaa taaagagctt tatcgaagat
     8881 caagatttat tagatgatga attttactcg attgtaaatg atcctatggg cgcaattatg
     8941 gaagtggact tttactacca gataccaaaa agctataaaa aagtctataa cattatgaag
     9001 acaacagcgc cagatttaga taatttagta aaaagtgcta tggatggtat ttttaacatc
     9061 tcagctatac gagatagcca tatcacaggc ttaattgctt ttaaatataa tgtggcgaac
     9121 gaaggaaaaa cagttgtgag gatcaaacga tattcagaat ttgagtggga tacttctgaa
     9181 ggattgaacg gagatatatg ggaagctaca tttgatttta aaccagtagc aacgccaaga
     9241 ccaaagtcta aatttagagg aaatgggatt atcacttact atccaaaaga gtacaacgac
     9301 tatttggaaa atatcaagaa tacgcttaaa gagaaagatt tggtaaatga tcaactgaaa
     9361 aaggtaatga atgctcctat gggggttata gctcagatag attattttta ccaagtgaga
     9421 aaagataaga aaaagcttga ttcactgatg cgtacttcac agccggatat tgataattta
     9481 gtcaaaggaa cattggacgg aatttttaac aaggaaatag gcatcaaaga tagcagggtt
     9541 gtagggttaa ttgcttttaa atacaatact tttgaaaagt cgaaaacaaa cgtgagacta
     9601 caggagatgg gctgatgaat aataatgaaa aaattcttca tgtacttgat agctttgaaa
     9661 taattcaaga agaactaaaa aaatatcggg atgttttgga acaaagatat gattttgtca
     9721 atcagcaaaa gagtaatcac atggatttca ttctcaatat gaatgacttg aagaagaaat
     9781 tagttgaacg aaaagaacaa gaaaagttga tcaaagctta ctttgaactc ggtgaaaaag
     9841 aagttaaaaa tgcaatggaa ctgaatgaag agcgcagggt tttagatcag ctgttagaac
     9901 aattattggt gatgtttcaa aaaggacgaa tagatgagga tttaattgaa gaaggattga
     9961 gaaaatatcc ttcaaactca ggtatcggaa tagtgctgaa agcgattgat gaagaagaaa
    10021 ttgaagcttt cataccgcca gaggattttg aatcggcaat ggaatatata aaatattata
    10081 gccaagggat cactgctttt agagagtttg atcctgaaga tgttattcat gacttgaaca
    10141 acctaaaaga atggtgtgaa agttatggag tcgatgattc cggattggac tacttgatca
    10201 gtattatgga aatcgaagaa gagatgccgg ataagcctga tccaacagat attttagaat
    10261 tgatccatga agctagaaat ccaattgcct atatttctag aggatatact gtattggaat
    10321 actataagcc ttatatatca gcgatgaacc atttaagacg tgtattaagg gaaaaacgtg
    10381 aataccgctc agtgttgaat gcaacgaata gattagaaaa agctgtttca gagctagatg
    10441 cttattatcg tgaacattac ctgcaagcag gtggtatgcc aagaaatacg aaagcaaata
    10501 tcagtaggta tataaaaaaa gctgagtagg cggagttagc ccataaaatg gcaaaaggta
    10561 acctgaaagg ggcattttga atgaaaaggg atgtttttga attgtacgta ttagaacgtc
    10621 tagaagacgg catgatggca caagagataa ttgatgaaat tgatgatatg atcagcagat
    10681 ttggtcaatt ggagttgaat gatggaaaca aaaattcggt agtgctaaaa caattttggt
    10741 tgaacaattt ttcgtataag gattttatca aagaaaaggc aaatacattg tactgactaa
    10801 attcacgagg agcagtgatg agagcttatt gctaagagca gttaggctct tttttgtttg
    10861 tgtaaaatta tttgttttat aatacacatt tatacacaaa aaaatattgg cagatagtac
    10921 acaaatgtgg taggatatat ttgtaagcaa gttagattaa caagttaaag gaagagcagt
    10981 tatgaaaaaa gaagaactaa tagatatatt tcaaattgtt gaacgagcaa ataatatggg
    11041 aattatgttt tttgatcgta tttctttaaa gatg
//
LOCUS       DAGCYL010000088        10930 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.089, whole genome shotgun sequence.
ACCESSION   DAGCYL010000088 DAGCYL010000000
VERSION     DAGCYL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.089"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            28..548
                     /locus_tag="MB216_001724"
                     /pseudo
     CDS             28..548
                     /locus_tag="MB216_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(450..1166)
                     /locus_tag="MB216_001725"
     CDS             complement(450..1166)
                     /locus_tag="MB216_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBT4526560.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1216..1425)
                     /locus_tag="MB216_001726"
     CDS             complement(1216..1425)
                     /locus_tag="MB216_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526561.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            1702..2975
                     /locus_tag="MB216_001727"
                     /pseudo
     CDS             1702..2975
                     /locus_tag="MB216_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3082..4113)
                     /locus_tag="MB216_001728"
     CDS             complement(3082..4113)
                     /locus_tag="MB216_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4526562.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(4120..4950)
                     /locus_tag="MB216_001729"
     CDS             complement(4120..4950)
                     /locus_tag="MB216_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4526563.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4947..5762)
                     /locus_tag="MB216_001730"
     CDS             complement(4947..5762)
                     /locus_tag="MB216_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBT4526564.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5759..6583)
                     /locus_tag="MB216_001731"
     CDS             complement(5759..6583)
                     /locus_tag="MB216_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBT4526565.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6573..7673)
                     /locus_tag="MB216_001732"
     CDS             complement(6573..7673)
                     /locus_tag="MB216_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBT4526566.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7851..8636
                     /locus_tag="MB216_001733"
     CDS             7851..8636
                     /locus_tag="MB216_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBT4526567.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8669..9052
                     /locus_tag="MB216_001734"
     CDS             8669..9052
                     /locus_tag="MB216_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBT4526568.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9128..9259
                     /locus_tag="MB216_001735"
     CDS             9128..9259
                     /locus_tag="MB216_001735"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4526569.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9228..9464
                     /locus_tag="MB216_001736"
     CDS             9228..9464
                     /locus_tag="MB216_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4526570.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9542..10513)
                     /locus_tag="MB216_001737"
     CDS             complement(9542..10513)
                     /locus_tag="MB216_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBT4526571.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3258 a         2535 c         2103 g         3034 t
ORIGIN      
        1 acatcaccct accaaaggag tcgctctatg gataatcata ctagaaaatt actcggatta
       61 acagataaac atttattttt tgatgaagaa tggctgattg aaaaagaata taaaggcgtt
      121 caagcgcagt ttattaaagg gagattggat gactcacctt cccattgtga acactgtgga
      181 tcgattcgta ttatccgcaa cggttcctat acgacacgta ctcagatgtt gaaagtcaaa
      241 gaaaagttga ctattttaga attaaaacgt acccgttttc tatgctatga ttgtgggcag
      301 accttttctg cgaaaacgga cttagttgat gaacatcatc aactaacaaa ggaactgaag
      361 caggcgattc tcatagaact gtacgaaaat caatcacgta atctgaaaat cagaagaagt
      421 accgtcggct caagcgatat tggaaactct tactcaaaga ttccacgaca ttggagccgc
      481 tcaaacggca ttatcatcga ctgtttaaac gaccaattag tcaaacagaa atcgtcgatg
      541 aaccgttgtg cacaatggtg tcctgaacgt acagcgacac cttccatatc cagagcagtc
      601 gccacatcac ggggatggat gccatccaaa ttaaaagcaa ccacgccagt atgatgttca
      661 ggatcttgcg gtccgtaaat ggtgataccg ccgatcgctt gtaatttagg taaaacgtag
      721 gcaaccagtt cttgctcata acgatgaata ttctccatac caatctcatt caagtaatcg
      781 attgctcctg caatgttcgg agtaccagct tcaaatttcc atggcagctc tttccatgaa
      841 ctatcgtaaa gattgacaaa atctatcatt tcaccgccga attcgactgg ctccatttgt
      901 tctaacaatt gacgtttgcc atataataca ccgattcctg tcggcccgca cattttatgc
      961 ccgctaaagg cgaaaaaatc tgcatccaac gcttgtacat caactgtcat atgcggtgct
     1021 gcttgggcac cgactggact atcctcaact acaacagcct gttcaaccct cagccgtttc
     1081 aagcacaaca aataaccttc tggatctggt ttgccgacct gaagatcatc gatcgtcaca
     1141 aaggaatcaa ttttttcctt aatcatcgga tgtgcttcaa aaaaatagtc tagctcccgc
     1201 agttcagaat tgagactact ccgattttcc agtgctgctg atccgctgct caaaatgttc
     1261 agcatccact ggtctcccaa ccgcttgact tatcccttca taaaaggaca gttctgctgc
     1321 cgtcccaaca ccttgttgat agtctgcaaa aggaatttta tagccgaact gctccagtaa
     1381 ataggcttca gtaaatagtg atgaatactc tcgtaaaaca tccattcact gtcaatcatc
     1441 gtcccatcaa aatcaaaaat aagtgctttg gtcatcttct ttccccctta cccaaaacaa
     1501 gcaaaagctg tgtcctgctc tctctacagt atcagcataa aaactgccgt ctgaaaagtc
     1561 aaaggtgaaa gaaatgtggc gctataattt ctaggactga tagtaaatca ttctatcagt
     1621 ccttttgagc ataaaaaaac aaaacataca gaagctccgt tagaattgaa tcaccacaac
     1681 acaatcaaag gagcgttctg tatgcctgat cttcatctta tcaaactatt actaggaatc
     1741 aaagatggaa acattcaaat cactcatgtc gagaagaaaa aaatacattc aatcaacagt
     1801 atcgttgttt ctgccacgct cattaaaacg attcagccct gtcctcagtg taaacaggtg
     1861 aatcaacatg ggctgatcgt taagaacgga aagaaaaaat cgctggtcct tctcaataag
     1921 tgtgcgaatc aacggactta tcttgctttg gcaaagcaac ggtacctctg tcgtggctgt
     1981 cacacctatt ttacggcgaa tacctacatt gttgaaaaaa actgttttat cgccaaacaa
     2041 gtacattata aaatattgga ggaattgact gaaaaacaag caatgacgac gatttctaaa
     2101 cattgcggcg tttcttggtc aaccgtttcg cgaacactcg cttctctgct ccccatgaca
     2161 aacgtgaaaa taaattggtt gcctcgctgt tcattggtag atgaatttcg ttcattgaaa
     2221 aatcaagtcg gtccctattc tttttcttgt atggatggcg atactgggaa attactggat
     2281 attcttcctt cacgtaagaa gaaagatctc atttcttatt ttatgcaatt tgaacgacgt
     2341 gcacgtttaa acgtaaaaat actcgtaaca gatatgaacg cctcttatgc tttgctgata
     2401 aaagagtgct ttcctcatgc caaactcgtt gtggatcgct ttcacatcgt caagcacctc
     2461 attcgcagtt ttaaggatat ttgactatga gtcatgaaaa gcttcgaccg aaacgatcca
     2521 atacaagcca aacactatcg acaagtgaag gctttatctc gacttctgat caaacgacag
     2581 gacacgcttg tttatgataa atggacaaaa tggcgtaatt ttggttgggc ctatttaacc
     2641 gaaagtgaag tcgtagaacg tctactttct acttctgatg aactacgaat agcttatgct
     2701 tactatcaag aaattcttca agctttttat gacaaagaag cggacatctt ctttcagctt
     2761 gtaaaaacaa tgcccaagtc ggttccaaga gaactgcatc atataaaaaa agcgtttata
     2821 aactatgaat ccggcattcg tttagcctta gagttgcctt actcaaatgg caaaatagaa
     2881 aatctgcata cccacatcaa agcattgaaa cgaatagctt acgggttagg atgagaacca
     2941 gaattttctt aatcaatcat ctgatcactt attaaacata aaaaaacatc taaagcagaa
     3001 aatcaattct accttagatg ttcttatatc actctatcag tcctatttga caaagagcca
     3061 gaaatgtttg ccgactatac actagctttg ttgtgcaaaa tgctgagtat caatttccac
     3121 agggattgct agaatatctt tcagctgaat gtataaggca tcgctaattt tctcttcccg
     3181 atcgttcaac cgatccacta aaatttcttt gccctccagc gtttgaaaaa cataccgttg
     3241 aatgttgcct aaaataaatg attccttcca tagaaccgga atcttaaccc cctgaggaac
     3301 cggttcaaat tgaatcagct ccggtcttaa ataataaatg cagcgattgc ccttagcgga
     3361 aaactcgtgg gtatactgat tcagctcata ccattcgaaa cgattatagt tgccgataaa
     3421 ttccgcaaca aatcggctct gaggattctg atacaattcc ttcgaggtgg aaacctgagc
     3481 cacccgccca gcttccatca caaaaatccg atcactgatt cgcatcgcct cttcttggtc
     3541 atgagtcaca aaaatcatcg tcatccctag cgattgctgg atagcgcgca aatcccgctg
     3601 cagctgtttt ctaattcggg catccaaagc acttaatggc tcatccagca gcaggacttt
     3661 cggttcagtg accaatgagc gagccagtgc cacccgttgt cgttgaccgc cagaaagaaa
     3721 ctggacattt ttatctgcct gctccgtcat atccaccaat gatagcatct caaaaacctt
     3781 ttcctgaatc acctcaggac tcagcttctt taccttcaaa ccaaaggcta cattttcaaa
     3841 aacagtcata gttggaaaca atgcatactg ttgaaaaaca aagccgatat tgcgatcctt
     3901 cgtaggctta tcggcaatat tctcattgtc cagcacaatc tcaccgctct ttggttgttc
     3961 caatcccgtg atacagcgca gcaacgtaga ctttccgcac ccgctgggac ctagtaaagt
     4021 aaccagctca cccttttcaa tctgaatttc atcaatagct aaaatctgtt tttcatcaaa
     4081 aaacagattg agatcatgta tttctaaaaa cattttccgt cacaactcct ttaagggctt
     4141 ctttttcgac tgcttctctt ccctcgtctg cttaagggac agctgttgct tttgtcgata
     4201 agtcagcata aaggtagtta aagcaattcc caccataaac agcaaataca aaaccatcac
     4261 agcgctagcc aaatggccgt tttcgttcat ccgccgcagc atatagatgc gtaccgtctg
     4321 aaatcgaccg ccaatcaaca ggttcgtcag catgtactcc ccaaaaaccg aagaaaaaat
     4381 catcagagaa actagggtca caccgatacg gatgtttgga aacagcacct ggataataat
     4441 cgtagacatc ggggcgccca aggtttctgc tgcatccact aattccttca gattaattaa
     4501 gcgcatctgg ttgttaagac taagatagac aatcggcaac gccgtaataa acaatgcacc
     4561 taccaacact acaaacattg ggataccagt ttcagcatac gttcgaagca aagccgttac
     4621 caaaatcact cctggtaaag catacggcag caggagcagc ttttccagcc aacgattcaa
     4681 acgagggaaa tacaaatgaa tccaaataat ggtgggaatc atcaacaaca gaatcgtcac
     4741 caagaccaca cctgctaata aaagtgagcg acccaccgca gcaataaatt ccctgtccgt
     4801 cagcagctgt tggaaccatt gcaaggtaaa atcactaggt aaaagcgatt ttgaccattt
     4861 agtggagctg gcatacaaaa tcgttccaat aatcggcagc aagtaataca ggacaaagca
     4921 gaacaaaatc ggttttatta actttttcac gtctgcacca ccttgccttt cttttgaata
     4981 atcaacattg ccgttaccag caggacacct aaagcgactg ccaacgcact gcctaaatta
     5041 ggcttggcga acacatctcc cgacactaaa gccgcaattc gaatcgttaa aagatttaca
     5101 ttactgctgg tcagggcata cgctgtttca taggtcccca tgccattagc aaataagatg
     5161 acaaaggtgc tggcaatcga tggtcgcaaa aaaggcaaag ccaccttgaa ccaagcatac
     5221 gctgatgaag cgcccagcaa ttgtgcggac tccagccatt cctttttcac ttccttcatg
     5281 ctgggataca aaaacaagac ccctaacgga atttggaaat aggtatacgt gatggtcaat
     5341 ccccaccaag aatacaaatt gaagtccgct agaaatggca aaatcatttt cagcacacct
     5401 acattcccca acaaaataat aaaagagaat gccagcggaa tcccagcaaa attagctgcc
     5461 aaattggaaa caaaggtgat tttctcctgc aatttagtcg atagattcat taagcataaa
     5521 cataaaataa acgtcccgat taacccaatc acactggagc tgacagcaat cagtacgctg
     5581 ttaacaaagg cctgtgtgta atatttgttg ctgaaaatat cactgtaatt ggccaatgtc
     5641 cactggttag ttaaatcact gtgcaagctg cacagaacca taaaaagcag cggcagcagt
     5701 aaaaatgcac agaagataaa ggaggcagga accagaacca agcgtttttt catctctttc
     5761 atcccctcat caattcttga atgtcctgca ttttctctga cggtgcaatt cctaataacc
     5821 agcaaaacaa tggagccgcc agcagctgtg gtacagtttg ttccttcagc ttttggcgtt
     5881 tatttgaaat caaataaaaa ggaacttcac gatgtgcagc caagctgccg ccatgcagtc
     5941 cgtgcgcatc catcccgtga tccgcagtca ccacgacttg ataacctttt tgcagccatt
     6001 caggaaggta aatagccaat aatctgtccg cctgattaac cgctgctgca tattcatgag
     6061 aatcggcggt aaatttgtga ccgacatcat caatattcat cgaatgtacg accaaaaagt
     6121 ctggctgata ctgattcact aaataatggg catccgcaaa taaatgcgaa tcagggtaag
     6181 tgtcctcact ataaaagatg ccatgctgaa tcagtcgctc ctcctgatgc tggatgcgat
     6241 caaccaaacg attatagggg gcttgattgt acaactcgct gtaccagtgg taagcggcgg
     6301 ctcctgtttt tccgccagct tccttaacta attcaaacag cgaccactct ttggatcgct
     6361 gagcatggta attactatag atgccatttt cataggctgg aacgccggtc atcagtactt
     6421 cgtataacgg acgtgaattg ctgggcagct cactttttac cttcaataag cttgcttgct
     6481 ggtgctcaac taaatgattc atgaacccaa gctgggcgac ggctgtgtcg tagcgacagc
     6541 cgtctagtat aactacaatc agtttgtcaa gtctactttg cataggtcag tacctccgcc
     6601 tgccaaagct ctggaagctc tacagaggtc ttgttccaag cttcattgtc ttcaactggc
     6661 accgccgctt cataagcatc ctccggcagt aatttttcag ctacttcttt aggcagctct
     6721 accttgcgaa taggtcgcgc atagcccttt gccaaattga tttgcccttc atcagataaa
     6781 atatattccc gtgctagctt tgccgcattg ggatgagggg catttttatt gattaaggtt
     6841 gtatacccgc taatgatgga gccgtctgtt ggcacagtta cctcaaaacg ctcggagtca
     6901 atttggtcac gataatttag tccattgaag tcccaaacaa ctgccacatc gatttcacct
     6961 ttttcaagat ttgctacgct ggcatcaacg gtggacaaac ggcctttttc tgccattttt
     7021 ttgtagtaat tcagacctgg ctgaatatcc ttttcgtcac ctccgttggc aatcgccgca
     7081 gccaatacag caaattgtgc ttggttggca atcgtcacat cccccacgct aacttgatag
     7141 tcaccctctt gcagctcctt ccagctagtc ggtgcttgct tcacattctc tttatcggtg
     7201 ataaaggaaa tggttcccgt atagcttaac aaccaatccc catcatcatc ctttgcccaa
     7261 tccggaattt cctcccaata gttggtctta taaggtaagg ttaactcttt gctttcagcc
     7321 aacggaccga agctgatacc gacatcacca atatcggcgg ttccattttt tccttctgat
     7381 tcaaacttag ccaattcttc tgcactggac atatccgtat ccacgtgact taagccgtat
     7441 tctttttcaa ggtcgttcca agtgcctacc caatttgccc aagtatccgg cattcccaag
     7501 ctggcaatct ctccttcttc cttggctttt tctataatcg tatctaaaga gtcgtctgcc
     7561 gcagccgatt gcttcgttgc ttcacctgag acgcagcctg tcagcaggat acttactgtt
     7621 gctgcacaaa ataaaccgat agtcatgtta cgaatttttt cattcatttt cattcattct
     7681 tctccccttt cgataattcc attctaacaa agctttgtaa tgagagaatc agtttcttga
     7741 aaaggtttca tgaacaatgt aaaggttgta tatggtttgt aaatttcgac gaattcccgt
     7801 taaccttcta aagcttttgc ggtatgatag tagaaaaaag gaggctccct atgcagtatt
     7861 atgatcagca tctccacacg tatttttcct ttgattcaga ggaaaaattt gaaaactatt
     7921 tagcctatca gccggagttt ttcgtttcta cggatcactt tgatttaaac aatccatcta
     7981 ccggaaaaga tgacattccg gattatccag cctatataaa caaactggac tctttaaaag
     8041 aaacctattc caccaaattt ctgcgaggta tcgagattgg cgttgttcca ggacaagaag
     8101 agcggatcgt ggattacctc gctcagcatc cttatgattt aaagctggta agtattcatc
     8161 aaaatggccg ctttgattat atggacgatg tggttttaac caaagatccc attcaaacta
     8221 ccaaggaata ttttgatcag atgttgcagg tcttgacgga ttttccagaa ggagatattt
     8281 tgactcactt tgattatgga ttacgccgct tcaccttcac agcggaagaa ttcgcggagc
     8341 attttgaggc tcagctgcta acgattttca aaaaggttgt tgaacgagaa ttggcgatgg
     8401 agttaaatgc caagagcttc ttgaaatacg gcaacgaaga cctttaccgt tatgccgttc
     8461 ctttgtatca aagtgtcggc ggaaagctct ttaccttagg ctcggatgct catgttgccg
     8521 aagattatca gctgggcttt aaaaaaatga gtcagctgtt ggcagactgt caggtagaaa
     8581 agttgctagt cattcaagga aaagaacgcc gcctgacgcc gctgccgatt ttgtgattcc
     8641 tatataatag aagaaagtgg tgaaaaaaat gagcaacctg tgggactaca atcaagaggc
     8701 acccatccat tatttaattg cgcgacattg ggatgcgttg aaaattgagg cggtttgccg
     8761 atccttgtta gcagccgttc caaaacaaca actggaaaat tttttggtgg ctgacagctt
     8821 gcagcgggaa aaagttcagg cttattttgc agcctttaaa gatcagcctt tggaatattt
     8881 gcatgcgcag tttcatcttt tttatcaagt agccgcccca gatgactaca acgatttacg
     8941 aggacagctg caattgacct ttcaagcgga tgaaaccgcc tataccgttt tattgggaat
     9001 ggctcgcctt ggtgatcagg cgaaggtcga gtggcggatt ttcgatattt gatggcagga
     9061 tcaaggcgtt tcagttgcaa accttcctct ctttcaatta gaaaaaccgc agcaggaaga
     9121 agtttgtgtg ctggtcaccg atgctggtga aattcgtctc cgcttatttc ctgaccaagc
     9181 tcccctggct gtagccaact ggcgctccct agctgagcaa ggcgtttatg acaacacacc
     9241 tttcggctcg tgtcattaag gaatttgtta ttcaaggtgg cgctttggat ggttctggtg
     9301 atgaagctgt ttccagctat gagggctttt tcgctgatga agtgcatcgc ggactttatc
     9361 attttaatgg cgccttggcc ttaggggacc acggccccca caccaacggc aaccagtttt
     9421 ttattgtgca aaacacaaag gcgcaggctg acctgctaat gtaaagcaag catacttgga
     9481 gcttggagga ctaccggagc tggatggtcg ttatactgtt tttggacagg tataccaagg
     9541 gttagaaatc gtagcagcga tcgcagctca gccgacaaac agtgaagatg taccgattga
     9601 gccagtgttc attcgacaaa ttaacttcga aaaaacatcg taaaaagaca ctcactgact
     9661 tcagcaaaag ctctattctt gagctggctg cagctagtga gtgtctttca tttatccata
     9721 agataaagct tggattcaat aaataaataa atgttttggt taaagctgtg accagcaaat
     9781 gaggaaccgt gcgagccagt tgatctgcca atgcaaacat ttctttaggt gaaggcgctg
     9841 gcgtgtctgc caacggatac tccagtccca atgcttggta accgtgcata ccatagcagt
     9901 ggtacggtag taattctaac ggtacgaacc gatcaagatc tgccacaaaa caagcaatcg
     9961 tcagcagctc ggtcaggttc atattgatac ctggaaccac aggaactcga attcgcactt
    10021 cgcagttagg atactcaatt aaaaagcgac gaatattctt caaaatttgt tggttatcta
    10081 agccggtcca ttgtttgtgg agcttgctgg taaacatttt tacatccaca aacaatgtat
    10141 ccacataggg agctaccttt tggatttcat tataagcacc acaaaagctg gtttccagta
    10201 cggtattgat tccaatgtat ttcgattttt gcaaaatttc tttagcaaaa tccgcctgaa
    10261 ccagtgcctc gcctccgcta atggtcactc cgccatccga gtgaaagtaa aaaacagcat
    10321 ccttctcaat ctcatccatt acctcttcag cggtcatggt ctggccaaat cctttttcaa
    10381 tctgcttgct ttgtgattcg ggaatcgaac accaggcaca gcgtaatggg cagcccttta
    10441 aataaacaac ggtccgtaaa ccagcaccat catgtagaga accctgttct attcttaata
    10501 ctgttccttc catatagatt tttgaattac acactagttt aaactaaaac acacaaggaa
    10561 aaacttacca tttaatcata aaattagctc atataaatat actaggatgt aaaatagact
    10621 ggtcaaaatc tggtcaaaaa agaggactaa tacaggtagt cctctttttt gttattttaa
    10681 tattctaaat cattttttat tggtttattc ccttattcga tgtagactca aatcactata
    10741 tcactagata tcaaacggcg tcatacggaa tcaaaaacat caaatttgac ctgttaaatg
    10801 gtcattgaat aacattcaaa atcaataaaa cggagtcaga cacggagtca aaaacgctga
    10861 gagtaaaaaa tatatacagc cttgtaggta aagtaccaca gggctgtata ttttttattt
    10921 cagattacct
//
LOCUS       DAGCYL010000089        10922 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.090, whole genome shotgun sequence.
ACCESSION   DAGCYL010000089 DAGCYL010000000
VERSION     DAGCYL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.090"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..557
                     /locus_tag="MB216_001738"
     CDS             <1..557
                     /locus_tag="MB216_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526572.1"
                     /translation="FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIY
                     DDHYDSDGTCKVSIEKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGN
                     SIDGVRKVNRSLILEIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRR
                     FKLYVLQKDYASNGKYSKKYRIFI"
     gene            595..984
                     /locus_tag="MB216_001739"
     CDS             595..984
                     /locus_tag="MB216_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4526573.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            968..3418
                     /locus_tag="MB216_001740"
     CDS             968..3418
                     /locus_tag="MB216_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4526574.1"
                     /translation="MMYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHD
                     NFRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSM
                     IGDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEE
                     IRRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKS
                     ETLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQ
                     QQQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSV
                     DELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMG
                     LHKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKP
                     ALASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWK
                     ETLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEK
                     FPVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGS
                     ITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNRE
                     IFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGL
                     KFAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVF
                     EELFHAFDTRPPVQERME"
     gene            3423..5531
                     /locus_tag="MB216_001741"
     CDS             3423..5531
                     /locus_tag="MB216_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBT4526575.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            5528..6532
                     /locus_tag="MB216_001742"
     CDS             5528..6532
                     /locus_tag="MB216_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBT4526576.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            6551..7462
                     /locus_tag="MB216_001743"
     CDS             6551..7462
                     /locus_tag="MB216_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4526577.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            7649..7936
                     /locus_tag="MB216_001744"
                     /pseudo
     CDS             7649..7936
                     /locus_tag="MB216_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8124..8339)
                     /locus_tag="MB216_001745"
     CDS             complement(8124..8339)
                     /locus_tag="MB216_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526578.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8598..8765)
                     /locus_tag="MB216_001746"
     CDS             complement(8598..8765)
                     /locus_tag="MB216_001746"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4526579.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(9110..10549)
                     /locus_tag="MB216_001747"
                     /pseudo
     CDS             complement(9110..10549)
                     /locus_tag="MB216_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         3550 a         1953 c         2215 g         3204 t
ORIGIN      
        1 atttttattt tgtattttgg gtagcagcaa ctttacttgg ttatgataaa attcagattg
       61 caggaattcc tatacacatg caatataagt tggttttgag tggaattttt tctgaggtgt
      121 tgccagatat atacgatgat cattacgaca gtgatggaac atgtaaagta agcattgaaa
      181 aagaaaattt cgatgacatc gatggatatg actctgtgaa cttactgatt attgatactt
      241 acgatataaa aatgagtgaa ttatctatgg aaaatcaaac atatcctacg ataattgtta
      301 gagggaattc aatagatgga gttcgtaaag ttaatagatc tttaatcttg gaaataaaaa
      361 aaacaatgga tgaaattcaa aaaagtgact ttaaaaaagt atttgttgct tcaacatcta
      421 atccaaaaaa tagtatcaat attattaatt catcgtttag atttttcggt cgatcacgta
      481 ggtttaaact ttatgttctt caaaaggatt atgcaagcaa tggaaagtat agtaagaaat
      541 atagaatttt tatttaagaa ggaatttttc cttcttttta ttttggagga aactatgaaa
      601 aaaataaaaa gctacaccag tatttgggca gttgaaaaag tcatttatgc gattaatgac
      661 tttcagctgc cttttcctgt gacctttaac cagatggctt ggtttgtttt atccttgatg
      721 tttgtcattg tatttgccca tgtgcctccg ctatccatga tagagggggc ttttttaaaa
      781 tacttgggca ttccgatagc ggtcacttgg ttcatgtcgc aaaagacctt tgacggtaag
      841 aaaccattgg gatttttacg atcctttatc agctatcact tacggtttaa ggtcactttt
      901 gcaggaaaga aagttaaaga acagaagaaa cgatgggacg aaccaatcac cttggtaagg
      961 agtgtgaatt atgtacccga ttaagtatat tgaaaataat cttgtcttta accaagaagg
     1021 cgagtgtttt gcttactatg aattagtgcc ttacaattac tcctttttgt cacctgaaca
     1081 gaagtatcaa gtacatgata actttcgcca attaattgcg caaaatcgtg aaggtaagat
     1141 ccatgcctta caaattgcca cagaaagtag tattcgtgcc acacaagaac gttcgaaaaa
     1201 ggaaattacc ggtagactca aagaagtagc aaagcaacga attgatttac agacagatgc
     1261 attagtatcc atgattggcg atagtcagat tgattatcgc ttctttattg gctttaaact
     1321 gatttcgaca gatgaagagg tcaatctaaa gaatctcagg aaatctttct tttctggact
     1381 tcaagaattt gtctatggag tgaatcatca tttgatgggt gattttgttt ccttatccaa
     1441 tgaagaaatt cgtcgctata ccaagcttga aaaactaatg gaaagtaaat tggctcgaag
     1501 atttaaagtc aaacgagtaa cgccgagtga cttgacgtat ttaattgaac atatctatgg
     1561 tgaaaaaggc acaccgtttg aagaatatga attccagcta ccaaagaaaa agctgaaatc
     1621 agaaacgctg gtgaaacgtt acgacttatt acgtcccact cgttgtttga ttgaagaaaa
     1681 gcctcggtac ttacggatgg aacatgaaaa tcatgagtct tatgtagcgt acttaacaat
     1741 taataccatt gtaggggaaa tggagtttcc ttcctcggaa cttttctatt accagcaaca
     1801 acaatttacg tttccgattg atacttcaat gaatgtggaa attgtgacga ataaaaaggc
     1861 cctagctacc gttcgtaata agaaaaagga attaaaagat ttagacaacc atgcctatca
     1921 atctgataat gaaacgaact ccaatgtatt agatgctttg gattccgtgg atgaattgga
     1981 aaccaccttg gatcagtcga aagaatccat gtataaacta agctatgtgg ttcgagtgag
     2041 tgctaaatca gtggatgaat taaaacgccg ctgtgatgaa gtgctcgatt tttatgacga
     2101 taccaatgta aaactagtcc ggccctttgg cgatatgatg ggtctacaca aagaattctt
     2161 accctcaagt aagcgataca tgaatgacta tattcaatat gttacgtctg actttttggc
     2221 gggacttggt tttggggcta ctcaaatgct aggagaatta gaaggcattt actttggcta
     2281 taacgtagat acggggcgca atgtttacct gaaaccggca ttggcatcac aaggggtcaa
     2341 aggctcagta accaatgctt tggcatctgc tttccttggt tcgttaggtg gggggaaatc
     2401 ttttagcaac aatctcttag tctattatgc cgtccttttt ggtgggcaag ctgtgattgt
     2461 cgatccaaaa ggagaaagag gcggttggaa agaaacgttg ccagaaattg cggatgaaat
     2521 taatattctt aacttgacca gtaagccaga aaatcaaggc ttacttgatc cctatgtgat
     2581 tatgaagaaa aagaaagact cagaaagttt ggcgattgat atcctgacct ttttaacagg
     2641 gatttccagt cgggatgggg agaagttccc tgtgttacgc aaagccattc gaacggttac
     2701 ccaaagtgat cagcgaggat tattacaagt aatcgttgaa ttgcgtaaag aggactcacc
     2761 agtcgcggaa aatatcgcgg atcacatcga gtcctttacc gattatgatt ttgcccattt
     2821 gcttttctca gatggttcga ttacccagtc gattagttta gataagcagt taaatatcat
     2881 tcaagtagcg gatttggttt taccagatgc agaaaccact tttgaagaat ataccaccat
     2941 ggaattgctg agtgtttcca tgctaattgt tatttccact tttgccttgg actttatcca
     3001 tagcaaccga gaaattttca agattgtgga cctggatgag gcttggagtt ttcttcaagt
     3061 agctcaagga aaaacgttat cgaataaatt agtgcgtgct ggtcgggcca tgaatgccgg
     3121 tgtttacttc gtcacgcaaa acagtgatga cttgttggat gaacgtttga agaataatat
     3181 tgggctaaag tttgcatttc gcagtaccga tattcatgaa atcaagaaaa ccttggaatt
     3241 ctttggatta gataaagaag atgaaagtaa tcaaaaatgc atgcgagatt tggaaaatgg
     3301 gcaatgcttg atgcaggatt tgtatggtcg agtaggtgtg gtccaagttc atccagtctt
     3361 tgaagaactt ttccatgctt ttgatacccg tccgccagtt caagagagaa tggagtgagg
     3421 agatgaaaaa gaaacttttt cgtatctttg gaattgtttt aatcagtttg gtcactctga
     3481 taatactttt gagcttagtt ggaacggtag ctgaggcaac aggtctggtt gatgatacag
     3541 ttggagccgg aaatctttat tcccaatatt cacttggtaa ctatcagttg gatttctttg
     3601 tggatagttc gtgggattgg ctgccttgga attggggtga tggcctaggg aagagcgtaa
     3661 tgtatggact ttatgccatt actaatttta tttggacagt tagtctctac ttatcaaatg
     3721 ccacgggcta cgtggttcaa gaagcctata agctggattt catttcagat acggctgaaa
     3781 gtattgggaa gaatatccaa accttagccg gcattaccga aaatggactc cagacctcgg
     3841 gcttttactt tgggtttcta ttattgatga ttttagctct aggagtttat gtggcgtata
     3901 caggattatt aaaacgagaa actaccaaag cagtccgggc tgtcattaat tttgtgatga
     3961 tttttctact ttcgggttcg ttcattgcgt atgcgcccac ctatatcaca aaaatcaatg
     4021 attttagctc agatgtcagc gaagcggctc ttagccttgg aaccgagatt gtcgtgccga
     4081 attcagaaag ccaaggaaag gacagtgtgg atttaatccg agatagcttg ttttccattc
     4141 aagtccaaca accgtggtta ctcttgcaat ttgatgattc caatatagaa gaagttggcg
     4201 aagatcgtgt taacaaaatc ctgtctgtaa gtccagatga aaataaaggc aaggatcggg
     4261 aagaagcggt caaagcggag attgaagata atgacaatgc gaatctcagt attaccaaga
     4321 ccatgaaccg cttagggacc gtggtctttt tggtgttgtt caatattggc atatccttct
     4381 ttgtctttct cctaacaggg attatgttgt tctcgcaaat tctctttatt atctttgcga
     4441 tgtttttgcc gattagtttc ttattgtcca tgttgccgac ttatgagagt ttgggcaaga
     4501 aggcgattat tcgcttgttc aatacgatta tgatgcgagc aggagttact ttggtgatta
     4561 cgactgcatt tagcatctcc acgatgtttt tcaatatttc ggcaacctat ccatttttca
     4621 tggttgcttt tctgcaaatt gtgacctttg ccggtatcta tttcaaattg ggcgacatta
     4681 tgaatatgtt caatctccag agtaatgata gccaatcaat gggaagacgg gtcatgcgaa
     4741 aaccgcaaat gttaatgaat cgaacattgc ggcaactcaa tcgaaatgtg ggacggactt
     4801 tggcttttgg tggtggtgca gcagttggaa ataagttggc aaaggaacaa tcaaaatcca
     4861 aatttaagcc atccggttct tctctacgaa agaattcacg attaccgaac gatcacgaga
     4921 taccatctga cttgaataag gaaaagccaa ttccaaataa taagaaacaa tcccgaatga
     4981 atttggccgg gagaaaagtc ggcaaggttc tggataccca agctttggtg aaagataaga
     5041 ccaaacaagt aaaggatgga gtacggaata cgccaaccaa cttgaaatac aacttccata
     5101 aaggaattga aaaaacgaaa aaagcaccgg aagagtttaa gcgaggactg gtccaagaaa
     5161 aagcaaatcg aggagagttg cgagaaaaac aacaacaacg tagagacgaa aaaatagctg
     5221 aaaagcgcaa agtattgaac gaaataggga atcctcatgg gaaaagaagg gaaaatgttc
     5281 cgataaaagc aaaggtccaa tctcaaaagg atagagcacc aaagcggatt gtgagagcaa
     5341 attcgaatcc cgaaatcaaa cgaaaattcg ctagccaaga aatcatttca aaaggaataa
     5401 accaatcctc aagtaagaaa aattcttttc aacaagtgaa acggcggtct actctgtcca
     5461 aacgaaccaa tcgaaaagta caaaaaagtt tgaaccctca tcccaaacca aggtcaggtg
     5521 agaaaaaatg aacgtaaaga aattaagtgc catttcgtta actttcctaa caatctcttt
     5581 tatgggtatc ttgatgtgtg tgggcttatt gtttggtgaa gacagtgaag gaagtagtag
     5641 ttcaggtgct cctcctgggg ggagtagtgt ctcagaagag gttttgaaac accgtgcaat
     5701 ggtggaaaaa tatgccaaag aatcggatat aacagagtat gttccgattc ttttagcgat
     5761 tattcaagtg gaatctggtg gcacaatgga agatgtgatg caaagttctg agtcagccgg
     5821 tttaccaccg aattcattaa gtaccgaggc atccatcaaa caaggatgtc tctattttgc
     5881 gtctttggtt aagcgttcca aagaattagg ttgtgatcaa gatagtatca ttcaggccta
     5941 taactttggc ggtggttatt tggactatgt ggcaaaaaac gggaagaagc atagttttgc
     6001 tttagctgaa tctttttcaa aggaaaaatc ttgcggtcaa aaaatcgatt accctaatcc
     6061 catcgcgaat aaggaaaatg gtggatggcg ctataactat ggcaatatgt tttattgctt
     6121 attggtgaag caatacctaa ctacaacaca gtttgacgat aaaacagtcc aagggatttt
     6181 tgacgaggcg tttaagtatg aaggaacggc ttatgtattt ggtgggtcaa gacccgaaac
     6241 aggatttgat tgtagtggct tgactcaatg gtgttatgcc aaagttggtc tgaaacttcc
     6301 tcgggtggct caagatcagt atgatgccat gacccatatt gacctaaaag atgccaaacc
     6361 aggggatttg gttttctttc attcgactta tgatgccggt acttatgtga cccatgtcgg
     6421 tatttatgca ggggagaatc gcatgtttca tgcaggagat ccagtaggct ggacgaatct
     6481 aacagaaagc tattggcagc agcatcttat cggtgctgga cggtacaaat aagagaaaag
     6541 aggaaaaaga atgaagataa aaattgaacg gaatcaaaag gaaaagagcc taaaaaagaa
     6601 aaaagaacga gtcgtatccg tgggtaagca tcgaaaaatg gtgcttgccc tttggctttt
     6661 attatcttgt agtcttactt ttggcgttta taaaaatttc accgccattg atcaacatac
     6721 aactcatgaa aaagtggtta ttaaagagaa agtagtgaat acttctggta ttgaaaactt
     6781 tacgaaggat ttttcgaaag aatatttttc gtggaaaaat aacaaagaag caattgaaaa
     6841 gagaatgaac aacctcgaac agtaccttac agaagaaggt ttcgctttaa gtcaagatat
     6901 ggttcgagca gatattccca ctaattccga agttcagtca gtaaaagtct tagatgttga
     6961 aaagagctcg gaggaatttg tcgtttcatt tttagtggag caaaagatta cagagggaaa
     7021 aaaggcacaa tcgatttctt ctgcataccg agtgacgatc tttgaagatg aaaacaggaa
     7081 ttatatagta acgagtttgc caactatgat tgcaaaacct gatagggcaa aatacaagac
     7141 aaaacaagta gaaaatgatt cgaaaattga tgccaaaact acagaagaaa tcgctgagtt
     7201 tttggaaacg ttctttaaat tatatccatc agcttctgaa aaagaattgg agtattatgt
     7261 tgaaaatgat gcgatgcgac caatcaatat tgacttaaag ttttcgaagt tagaggatcc
     7321 agtttaccta caaaacggaa aaaaaattct agtgagtaca aagataaaat accttgataa
     7381 tgtatcaaaa accactagct actttcagta caaattaata ttacaaaaaa atggaaactg
     7441 gaaaatcatt gatgttaaat aataatgact caatctaaat ttatgagaag ttataagtag
     7501 tttaacttat ttcaggcaga agtcgcccat cctctatagg tgggtgatga atgccgttcg
     7561 gtaactcgaa aaccgacaat taagacatag tgccttttta ttttaatcca tgtcataatc
     7621 agtctatata agaaatggtt gatataccat ggaattagat acaaataatc attcattatt
     7681 tcttctttgt taccacctta ttttagtagt gaaacattgt aaacatctca ttgatgatgt
     7741 aatgttagct tatgcaaaaa actatctttg aaagaatatc agaatcacat cacattacct
     7801 tagttgagtg aaatcgtgat aaagactact ttcatattat gctcaaagca caacctaaaa
     7861 ccgaattaac aaaattcagc aatgcctaag ctcatgtctg ataaaatgag actttctaag
     7921 ggtcaaaaat ttcctttgaa tacgatcatt agcttagata aaagtaaaaa ccttagaact
     7981 acggggatag cttggttaat aagagatatc gctgttaata aagacataag cttgcaagta
     8041 tgctctattc ccaagaatct cccacttcaa gcactaagga tcgttaaatt cagctaagtg
     8101 gcaagtagtt cactagacga taattatctc catatttcaa tctcagcttt tccatttttc
     8161 atagcaaatt ccgttatttt tatagcatct aaaagattca gcttttccac tgatctttca
     8221 ccacttttca gcttttttat tgtgcttaaa ttgataccag taccttttga tattgaattg
     8281 gaagtgaaat cattaagggt tttttttatt aaattcatat ccgcaattcc agcgttcata
     8341 agaatgaaga ttctcctctc atagactact ttgaaaatgg tactacagtg gctgtttata
     8401 tatttcagta ggttactgta gtggtcattt tattataatt agaaaaggaa cctaaatcaa
     8461 aaatgctaaa gaaaattttc atagtattaa acctatcatg actaaagcca cggaattctt
     8521 gataaaagag tatacttgca agcctatgtc tttactaaca gcgatatctc ttattaacca
     8581 atctatcccc gttgttccta aggttcttga tgttttctaa ggcaatgctt gtatacatag
     8641 cccttgatca aaatattgat actaacgttg acgtttcgat catgatgtgt acggcaaata
     8701 ggacaagtcc attctcgaat tgtgaatgac ttcttaccat ctgtgtgtcc acattctgaa
     8761 ccaatttgac tagacggaaa tcatttatct atcttgatga tttttttgac agaagtatcg
     8821 aaaatcttta cataatctgg gtgttgcatt cttatgcaga tgagagcgtt agctccgtat
     8881 tcataaccag ttgcctctaa aacgtagtcc atcggtggat acctagcttg cggttgccag
     8941 tggctttcgc agtcttcttg taatcaaatc gagcatctaa ttgagtacta gtattttgaa
     9001 gataatactt gagtttttcg gttttcagaa gttcacttca tcttccatca tccatggaaa
     9061 tcccactcac ccatacgcag gcctccaaaa attcttcaac aatggttttt cagacatttt
     9121 tctgaatacc tccttgtttt ttgatacgcc agatagtcag gaaaaaggca acaaaacaca
     9181 aaacaaagct gcctaaaaag gtaatcttca ttccatataa aaagacatcc ggccgatctg
     9241 cgatatagtt agttactcgt tggcccatct tagcagacat cgcttgatat aagaccgtcg
     9301 tagaaagcga aatcccgatg accatcccca aatttcgggc aaaggaattc atggagcctg
     9361 ccagtcccaa attttcgata gtgacagaac tcataaccat cgtattattt ggggactgga
     9421 acaaggcatt gccggcaccc atgatggcag tagcgacaat gtacagccac aaaggagagt
     9481 ccactgacaa aaacatatac atgacttggg ttaccgacaa taaacccaag cctactaata
     9541 caaaaatctc cgggccaacc cggtcggtca ggtatccgct taaaggcgcc ccgacgacca
     9601 tcatcaaggg aaagaccatc atcaacagtc cggcatagct aggactcaac ccgcgagcat
     9661 tttgcagata aaaaggaatc actacattaa caaagaagtt ggaagaaaaa atcaacaagg
     9721 ctgtaatcaa gctcatggta aaaatcttgt tcttaaaaat accgaaagaa atcaatggag
     9781 aggctgcttt cttttcaact tttaaaaagc tgacaaaggc aatcactgaa attacgaaca
     9841 gtacaagggg gactggatgg aaaaagccac tttcttgccc caaaaagatt gttccaaaaa
     9901 ggctgataat cgtagccgca atggcagcaa agccccctcc atcgattgta tggccgctca
     9961 tggtgatatc ttttggtaag aatttttcag caatcaacat cgttacgatt cccaccggta
    10021 cattgatcca aaaaatgtac gcccaagaaa attgggacaa aatcaagccg ccaagacctg
    10081 gtccagcaat cgagcctaat gaaacaagcg caccgattgc tcccaatgcc cgaccacgtt
    10141 catgtatggg aaagacttcc gtgatgattc cggcatttgt agccatagtc atactagcac
    10201 caatggcttg taccacccgt gcgaacaatt aaatacctag cgaatgattg aacccacaca
    10261 acagcgagcc caagacaaag atcagcgtac caatccgaaa tactttgatt ttcccacagc
    10321 tatcgccaat tttaccaaac agcaacaaac aagaacagac aatcatcaga taaacggaca
    10381 ctacccattc cgcttgattc attggtatgc ccatatcatc tgagatggta ggtagtgcaa
    10441 tattgacaat actgccatcc aatgtggaca tgaaggtaaa catagatata gcaatcaaaa
    10501 tccaccagcg atttttttgg acttttgcat cttcttgata actatgcata ataggatttc
    10561 ctttctaatg atcgtgtagc taagaacaat ataacctatg tctactgccc aacaacttct
    10621 cgcgactcta gccataggaa gcttgagtaa tttcgatttg ttattctcat gtaaccatgt
    10681 aagtttgaga tgctttattt gaacttactc tatagtaact agtgataatt cgctttactt
    10741 tcttctagca atttctaaaa gtcgacttca ttgttcttca gattaatagt gtgagctatc
    10801 tcgtgagacg taagaaaatt tccattctac tttttcccaa ttttttcata tttatggtgt
    10861 tgcacctatt tgttttccaa tttgcttttc aaatacattg ctatgatatt caattccata
    10921 gg
//
LOCUS       DAGCYL010000090        10822 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.091, whole genome shotgun sequence.
ACCESSION   DAGCYL010000090 DAGCYL010000000
VERSION     DAGCYL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.091"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="MB216_001748"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="MB216_001748"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBT4526580.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2479)
                     /gene="purK"
                     /locus_tag="MB216_001749"
     CDS             complement(1355..2479)
                     /gene="purK"
                     /locus_tag="MB216_001749"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBT4526581.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="MB216_001750"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="MB216_001750"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBT4526582.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="MB216_001751"
     CDS             complement(3376..4683)
                     /locus_tag="MB216_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBT4526583.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="MB216_001752"
     CDS             complement(4686..5267)
                     /locus_tag="MB216_001752"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBT4526584.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="MB216_001753"
     CDS             complement(5839..7131)
                     /locus_tag="MB216_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4526585.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="MB216_001754"
     CDS             complement(7128..7751)
                     /locus_tag="MB216_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526586.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="MB216_001755"
     CDS             complement(7754..8590)
                     /locus_tag="MB216_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4526587.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="MB216_001756"
     CDS             complement(8607..9494)
                     /locus_tag="MB216_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4526588.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="MB216_001757"
     CDS             complement(9510..10793)
                     /locus_tag="MB216_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4526589.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
BASE COUNT         3242 a         2456 c         1820 g         3304 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgcctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatcc tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat tt
//
LOCUS       DAGCYL010000091        10574 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.092, whole genome shotgun sequence.
ACCESSION   DAGCYL010000091 DAGCYL010000000
VERSION     DAGCYL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.092"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            12..84
                     /locus_tag="MB216_001758"
     tRNA            12..84
                     /locus_tag="MB216_001758"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="MB216_001759"
     tRNA            88..160
                     /locus_tag="MB216_001759"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="MB216_001760"
     tRNA            168..249
                     /locus_tag="MB216_001760"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="MB216_001761"
     tRNA            267..339
                     /locus_tag="MB216_001761"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="MB216_001762"
     tRNA            351..422
                     /locus_tag="MB216_001762"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="MB216_001763"
     tRNA            439..524
                     /locus_tag="MB216_001763"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="MB216_001764"
     tRNA            535..608
                     /locus_tag="MB216_001764"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="MB216_001765"
     tRNA            618..691
                     /locus_tag="MB216_001765"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="MB216_001766"
     tRNA            711..783
                     /locus_tag="MB216_001766"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="MB216_001767"
     tRNA            811..884
                     /locus_tag="MB216_001767"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="MB216_001768"
     tRNA            898..971
                     /locus_tag="MB216_001768"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="MB216_001769"
     tRNA            995..1084
                     /locus_tag="MB216_001769"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="MB216_001770"
     tRNA            1097..1170
                     /locus_tag="MB216_001770"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="MB216_001771"
     tRNA            1189..1264
                     /locus_tag="MB216_001771"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="MB216_001772"
     tRNA            1266..1336
                     /locus_tag="MB216_001772"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="MB216_001773"
     tRNA            1350..1423
                     /locus_tag="MB216_001773"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="MB216_001774"
     tRNA            1448..1535
                     /locus_tag="MB216_001774"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="MB216_001775"
     tRNA            1557..1628
                     /locus_tag="MB216_001775"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="MB216_001776"
     tRNA            1651..1740
                     /locus_tag="MB216_001776"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="MB216_001777"
     tRNA            1753..1826
                     /locus_tag="MB216_001777"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="MB216_001778"
     tRNA            1845..1917
                     /locus_tag="MB216_001778"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="MB216_001779"
     tRNA            1921..1993
                     /locus_tag="MB216_001779"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="MB216_001780"
     tRNA            1998..2068
                     /locus_tag="MB216_001780"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="MB216_001781"
     tRNA            2081..2154
                     /locus_tag="MB216_001781"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="MB216_001782"
     tRNA            2166..2237
                     /locus_tag="MB216_001782"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="MB216_001783"
     CDS             2503..3696
                     /locus_tag="MB216_001783"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBT4526590.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="MB216_001784"
     CDS             3826..5298
                     /locus_tag="MB216_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4526591.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="MB216_001785"
     CDS             5381..6169
                     /locus_tag="MB216_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBT4526592.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="MB216_001786"
     CDS             complement(6236..6904)
                     /locus_tag="MB216_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBT4526593.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="MB216_001787"
     CDS             7019..8731
                     /locus_tag="MB216_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4526594.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="MB216_001788"
     CDS             8734..10506
                     /locus_tag="MB216_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4526595.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3033 a         1926 c         2453 g         3162 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggca
//
LOCUS       DAGCYL010000092        10494 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.093, whole genome shotgun sequence.
ACCESSION   DAGCYL010000092 DAGCYL010000000
VERSION     DAGCYL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.093"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(128..613)
                     /locus_tag="MB216_001789"
     CDS             complement(128..613)
                     /locus_tag="MB216_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBT4526596.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    703..1048
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1093..1734
                     /locus_tag="MB216_001790"
     CDS             1093..1734
                     /locus_tag="MB216_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBT4526597.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1753..2388
                     /locus_tag="MB216_001791"
     CDS             1753..2388
                     /locus_tag="MB216_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBT4526598.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2406..3221
                     /locus_tag="MB216_001792"
     CDS             2406..3221
                     /locus_tag="MB216_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4526599.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3540..4976
                     /locus_tag="MB216_001793"
     CDS             3540..4976
                     /locus_tag="MB216_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4526600.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5165..7612
                     /locus_tag="MB216_001794"
     CDS             5165..7612
                     /locus_tag="MB216_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBT4526601.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7842..8909
                     /gene="rlmN"
                     /locus_tag="MB216_001795"
     CDS             7842..8909
                     /gene="rlmN"
                     /locus_tag="MB216_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBT4526602.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9102..10205)
                     /locus_tag="MB216_001796"
     CDS             complement(9102..10205)
                     /locus_tag="MB216_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBT4526603.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3461 a         1797 c         2278 g         2958 t
ORIGIN      
        1 tcgtattagt tttcagcgtt catactagtt gttacattgt ttgattggcc aaaagaattt
       61 ctgatatatt ttgtgctaaa taaagaaaaa gacgcttgaa ttgggaaata agaaaactac
      121 tctatcatta caaattttca taaactggca gatgttctaa gtaaacaagt atcttttcaa
      181 agtcggaaaa atccataatc aacgtagtgg tattgatatt tggatgaaag cctacggtat
      241 cttgacgatc aatagcagca tcgatcacga cttggatttt tttctctttg tcgtgcatca
      301 atgctaatgg agtcactgta cctgctggaa cgcctaggag gtcaggaagc tgttcttcag
      361 aaacaaaaga taagcgcttt tcttttaatt gttcagctag ttgttttaaa tcggcgtgct
      421 tttcatcagg caagatgaca agatagattt gttttccttt tcttgatttc aataatagat
      481 tttttacttg cggaccaggt agggaaaaag ggacattttt gactgacgta atcgcaggat
      541 gatcgacttt ttgaaaagag atacccagtt tttttagtag ttcataagct tcttgttcgg
      601 ttgcacgact catcgcgcat caccctcctt tttttattag tgtatcataa aaaagagttg
      661 acaaatgaaa aataatcatg taaattacac gtaaagttaa aacaaatgac atcgaaaaga
      721 agagtagccg ttaggtgttt ttagagagtc tctggttggt ggaaagaggc agcagttaac
      781 cgtgaaacac atcttggagt cgattttctg aagcgtatag taggaaaatc cggggagccc
      841 gttacagctg cggtcgttgc tagatgacca gataaggctg gcttttccgt gaggagtcgg
      901 taaaaaaagg tggaaccacg tgattaacgt cctttgatct tcggatcaaa ggactttttg
      961 ttttcatctg aacggcaacg actgtgtgag gttttttctg caaggaaaaa ataaacagag
     1021 gtggaaccac gtataatacg tcctcttggc atttgccaag aggatttttt tattttaaag
     1081 gaggaaaaga agatggagta tttgattgaa gttatgccgc aattattagt aggagcactc
     1141 acaacattga aattattttt atttaccttg attggctcat taccattagg gattgtttta
     1201 gcattaggat tgtctttgca ttttaagccg ctacgctact tgcttcaaac atatgtatgg
     1261 gttatgcggg gaacaccgct gttgttgcaa ctgattttta ttttttatgg gttgccgacg
     1321 attgggatcg tttttgatcg tttcgaagca gcactcattg cctttatctt aaactatgca
     1381 gcttattttg cagagatctt tcgtggaggg atacaatcga ttcctcaagg tcaatatgaa
     1441 gcagcgcaag ttttgcaatt aagtcccttt caaacggtga cgaaaatcgt catcccacaa
     1501 gtgacaaaga ttgttttacc ttctgtaggg aatgaagtta tcaacttggt caaagattct
     1561 tccttgatct atatattagg tttaggtgac gtaatgcgtg ctgggaaaat cgcgatggaa
     1621 cgcgacgcga ctttactgcc tttgctaggc gtggcaatca tctatcttac attgactggt
     1681 attctaacaa tcgtaatgaa acaagttgaa ggaaaattaa attattaccg ctaactgata
     1741 ggaggaagaa atatgattga attgaagaaa gtgaacaaag catttggtga aaaaaaaata
     1801 atcaaagatt tgaatctaat cattccagat gggaaaacat tagctatcgt aggtccttcc
     1861 ggtggcggta aaaccacgct tttacgtatc ttggctggct tagaaagttc tgacagcgga
     1921 gaattttatt tagacggtgt accgttcaac ccattcgagc ttaaaatgaa agaacaagtt
     1981 gtaggagtcg tattccaaga cttccagcta tttccacatc tgaatatttt tgaaaacatt
     2041 accttagcac caaaaatggt attgaaacag acaaaagaag aatacaccaa aaaagcagaa
     2101 gaattagcgg cttcattagg gattagtgat ttattgaata tctatcctta tcagctttct
     2161 ggcggacaaa aacagcgggt agcgattgcc agagcattgg ctatgaatcc acgggtattg
     2221 gcttatgatg aaccaacaag tgctttagat cctgaattac gccagcaagt cgagcgtttg
     2281 atcctatcat tgaaagaaga tggggtaacc caaattattg tcacccatga tttagtattt
     2341 gcagaaaatg tcggtgatca attactaaaa gtttccccaa ttcattaaga aaggagaaga
     2401 tagagatgaa aaaaaccagt tttctattcg ctttgatcgc aggtcttctt ctttttgcag
     2461 gctgttcgaa caaaaaaact tcagcagata attgggcgac tatcaacgaa gagaaaaaaa
     2521 tcgttatagg tttagacgat acatttgttc ctatgggatt tagagataaa gacgggaagc
     2581 tcacaggatt tgatattgat ttagccaaag cagtctttga acaatatggg attacggtag
     2641 actttcagcc cattgactgg tcaatgaagg aatttgaact gaacaatgga acgatcgatt
     2701 tgatatggaa cggctattcc aaaacgccgg caagagaaaa gaaagtgcaa tttacaaaac
     2761 cttatatgga aaacgatcaa gtgctgatca cacctaaaga atcaggaatc acaaactttg
     2821 gacaaatgaa taataagaga ttaggtgcgc aaaatgggtc atctggttat gatgtattta
     2881 cgaaacaacc agaagtatta aaagagatag tcaaaaacca agatgctgtt ttgtatgata
     2941 gttttaatga agcattgatc gatctgaaaa gcagacgggt cgatggtctg ctgatggata
     3001 aagtatacgc agactattat ttgaaacaaa gaaacgaact agaagacttc aacataacaa
     3061 aaggttctta tgagagtgag gattttgcag ttggtgcacg caagtcagat agtacattag
     3121 tgaaaaaaat caatgaagcc ctagatcaac tagagaaaac aggtgaattc caaaaaatat
     3181 ccgataaatg gtttggcgaa gatgttacgc cgagaaatta acaaataggg gactgtgaca
     3241 aaagacgtgt cacagtccct ttttccgaat aaacagtgtt ctcctgccaa ctccttgtga
     3301 ctcggagctg aatggcaggc tcacaacctc ttggtaaacg gtgttcaaaa gccagttttt
     3361 cggtattaag ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttac
     3421 tactcgaaac taagcgactt tttcacaacc tcctttacat atttttattt tgttgtggaa
     3481 ggggtttcgt gttattctaa aagaggaacg attgaaggtt ataggaaagg aagagatgaa
     3541 tggaacacaa agaacttacc tcatttccaa aagattttct gtggggctct gcttctgcag
     3601 cgtatcaagt agaaggagct tggcaagaag atgggaaagg agaatcagtc tgggatcgat
     3661 ttgtacggat acctggaaag acatttaaag gaacgaacgg tgatttggca gtagaccact
     3721 atcatcgtta taaagaagat attgctttaa tgaaagaaca aggtctgaaa gcttaccgct
     3781 tctctgtagc ttggacacgg atttttccta atggacgagg agaagtcaat caagctgggc
     3841 tcgcttttta tgagagattg atcgacgaat tgattgaaaa cgatattgaa cctatgttga
     3901 ctttatatca ttgggatctt ccacaagcat tgcaagatga atataatggt tgggaatcac
     3961 gtcagatcat tgaagatttc actcattatg cagaaacatt atttgaggca ttccgcggga
     4021 aagtccatta ctggattagt ttgaatgaac aaaatatctt cacttcatta ggttatttgt
     4081 tagcagcaca tccgccaggt gtgacagacc cgaaacgaat gtatgaagtt aaccatattg
     4141 ctaatttagc aaatgcatct gtcatcaata aatttcacga gttagaaatg cctggaaaaa
     4201 tcggaccaag ctttgcgtat acgccaaatt atccaattga cagtaatcct gaaaatgttc
     4261 tggctgctga aaatgcagaa gacctgatgg ccaattattg gatggatgtc tatatgtggg
     4321 gaaaatatcc gattgctgcc atgaggttct tagaagaaaa aggatgggca ccaacgattg
     4381 aagctggcga tgcagaattg ctagaatctg ctaaaccaga ttttcttggg atcaattact
     4441 atcaaacggc gacgaatgca ttcaatccat taaacggagt tggtgctgga aaaatgaata
     4501 cgacaggaaa aaaaggttca tcagaagaaa ctggagttcc tggaatgtat aaaaaagttg
     4561 agaatccatt tgtcgaacga acaaattggg actgggagat tgacccagaa ggattaagga
     4621 tcggactgcg tcgtatcaca agccgttacc gcatgccggt actgattacg gaaaatggct
     4681 taggagagta tgacaagttg acagaagata aacaaatcca tgacgactat cgaatcaatt
     4741 acttacaaag ccacgtcaaa gccatcaaag aagcaatttc ggatggagca gaagtcctag
     4801 gctattgcac ctggtcttat acagatttgc ttagctggct gaacggttat caaaagcgat
     4861 atggatttgt atatgttgat caagacgaaa cacaagaagg atctttggaa cgatataaga
     4921 aagacagtta ttactggtac caaaaactga ttgaagaaaa tggagagaac ctttagttca
     4981 catacttaaa acaagaaggt acggctggat atggccgtgc cttttcttga taaaatcatt
     5041 tagaaattaa gaaaaactgt aaaataagct gaaaaatgaa agaaagcgtc agttctttca
     5101 aacaaaactt tagttccctt actttttttt gtataatgaa acttgatgaa atgagggata
     5161 ataaatggca caaaagaaac ggcctactaa aaaaaggaaa aagacgaaaa aaacgaaaaa
     5221 gcaaaaacag cagcaggaac aaatgctgac gattggtgtt gggtttgcct tgattttatt
     5281 tgctgtcttc ggctttttaa aattaggttt tttaggaata ttgattgcaa acggctttcg
     5341 gatcattgca ggaaatactt accaaatcct ctgtctttta ttagccgtat taggattttg
     5401 gattgtcatt aagaatacag agttttcaat tggtaaaaac cgacgctggt ttggcggcat
     5461 actcttttat ttcggtatct tattattgtt acatgcccat ttatttggca aactccatac
     5521 tggggaacca aatatcatgg ggacgacatg ggatttgtta gccagtgata taaaacaaag
     5581 tcaagtagac aataacgttg gtggtgggat gattggagca atcctttatc attttactta
     5641 cttcttgatt gcacagcctg gaagttattt agcagcagtg ctattactgg caggaggagc
     5701 attcctgttc agtaatctgg aaggctatca gctgttaaat ggtatccaat caattggaga
     5761 acgagtacag gaattattgg aaggtgatcc tgccaagcaa gctcgaaagc aagcggcaaa
     5821 ggaagagaga atgaaacagc gcgcagaagc gaaagaagca aggcgcttag ctgcaaaaga
     5881 agctgcagaa aaagaagctg cagaatatga gaagaaaaaa gcggtgagcc agcaaaggga
     5941 aaatactgct gatgaatggc aggaaccaga aaatacagag ccggagcagc taagttttgt
     6001 tccaatcgac agttttcaag aaaatatcca tcctgcgaat ctagagaaac cagtaccaga
     6061 tacaccaaaa cagaccaata cagcagaagg tttttcagat gagttgccag aggatgacgg
     6121 tacctcatta gaatttgaga ttgaagcaga acaggaaaat caagactatg aattaccgac
     6181 agttgatttg cttgattcta taccaactgt ggatcaaagt gatgagtata aaaaaattga
     6241 aaaaaatatc ggcgtattag aacaaacttt ccaaagtttc ggtgtcgatg ccaaggtcgt
     6301 caaagccagc ttaggaccat ccgtcacaaa attcgaagtc cagccggctg taggggtaaa
     6361 agtaagtaaa attgtgaact taacagatga tatcgcgctt gctttagcgg cgaaagatgt
     6421 ccggatggaa gcaccgattc caggtaaatc actgattggt attgaagtac caaatggaaa
     6481 gatcagtatg gtttccttta gagaaatcat cgaagcacaa ccaaaccacc cagataaatt
     6541 actagaagta cctttaggac gtgatgtttc tggacgggta caaacggctg atctttcgaa
     6601 gatgcctcac ttactcgtgg ctggttcgac tggtagtggg aagtcagtag caattaatgg
     6661 gattatcaca agtatattaa tgcgtgctaa gccccatgaa gtaaaactga tgatgatcga
     6721 tccgaaaatg gttgaattga atatgtacaa cggcatacct catctgttga cgccggtcgt
     6781 gacgaaccct agaaaagcag cgcaagcctt acaaaaagta gtacaagaaa tggaagaacg
     6841 atatgaaaaa ttcgcggcaa ccggagtaag aaatatttct ggttacaatg aatttgttca
     6901 acagaaaaac ttggaaaacg gaacgaaaca tccaacctta ccattcatcg tagtgatcgt
     6961 agatgaatta gctgatttga tgatggtagc cagcaatgaa gttgaagatg cgattattcg
     7021 tttagcgcaa atggctcggg cagcggggat tcatatgatc ctagctactc aacgtccaag
     7081 tgtggatgtt atcacaggaa tcatcaaagc aaatgtacct tccagaatgg catttgcggt
     7141 atcaagcggt acagattctc ggacgatcat tgattctaat ggggcagaaa agctcttagg
     7201 gcgaggagat atgctattct tgccaatggg tgagaataaa ccgatccgtg tgcaaggagc
     7261 attcatttct gaccacgaag tagaacgggt cgttcaattt gttacagatc agcaagaagc
     7321 tcattatgaa gagaaaatga tgccgacaga tgaagttgaa acgacaggtg ctccagaaca
     7381 gccacaagat gagctgtttg aagaagcaaa agcacttgtc gttgaaatgc aaacggcaag
     7441 tatttccctt ctccaaagaa gatttcggat cggttacaac cgagcagcac gtttagtaga
     7501 tgaactggaa gctcatggag ttgttggccc gtcagaagga agcaagcctc ggaaagtatt
     7561 cattgagcag gaagaaacat cagaaacgtt gccggacgat aatgacatgt aaaacttgtg
     7621 tgatgaaggg ttgaaaaacg aataaccaat aaaaagacaa ggcatgatga taccaaaaga
     7681 aaaaatggtt tttttccatc ttttctaata cggtatgatg tcttgtcttt ttagtttttg
     7741 tgagattaaa gttctattcg atttatttgt gaagtattgc attaaggaaa tgaatcatgt
     7801 atagtatgta gcgaaacttt gagaaaaaga gggaaaatac tttggaaaaa cagtccattt
     7861 atggcttgac gaatgaagaa ttaatcaatt ggtttataga aaacggcgag aaaaaattcc
     7921 gagctgccca agtttgggaa tggttgtatc aaaaacgtgt aagtaatttc acagaaatga
     7981 cgaatctatc gaaacaattg atcgaaaaat tatcggagca ttttatcatc aatcctctaa
     8041 aacagatggt cgtccaagaa gcttcggatg gtacagtaaa atatttgttt gaactccctg
     8101 ataaaaacat gattgagact gtactgatga gacaagaata tggcttgtca gtttgtgtga
     8161 cgacacaagt aggatgtaat atcggctgta cgttttgtgc aagcgggctg ctcaaaaaga
     8221 accgtgatct gactgctgga gaaattgttg cacagatcat gatggtccaa cattattttg
     8281 atgagcgcaa tcttggcgaa cgcgtttcac atgtcgttgt aatggggatc ggtgagccat
     8341 ttgataatta cgacaatgta atggactttt tgcatatcat caatgatgcc aaaggattgg
     8401 caattggtgc tcggcacatt acagtttcca ctagtggatt agcacacaaa atcaaggaat
     8461 tcgctaataa cggcttgcaa gtcaatctgg ctatttcctt gcacgcgccg aataatgagg
     8521 tgcgaacatc gatcatgcgg atcaaccgaa gtttcccaat tgagaaattg atggaagctg
     8581 tggatgagta tttagagaaa acaaaccgtc gaatcacgtt tgaatatatc atgttgaatc
     8641 aagtaaatga tagaccagag catgcgcaac aattagcaga tctattaaaa gataagaaaa
     8701 aattagcata tgtcaacttg attccatata atccagtcag tgaacatgat caatattctc
     8761 gcagtccaaa agctgatgta ttgagatttt atgatgtatt gaagaaaaac ggcgtaaact
     8821 gtgtgatacg taaagaacat ggtacagata ttgatgcagc ttgcggacaa ttgcgaagca
     8881 agcaaatgaa aaaaacagtt gccaaataat cgtatcctac tcgttttttc aattaccata
     8941 aaaggagcag ttttcttaat taaggaactg ttcaacttaa ataagagaaa agattctata
     9001 gcgaaagcta taaagctttt tttgacaaga aaaaccctca tcaagcaaag aaatgtcctc
     9061 aatttgatga cagcctgctt gatgggggtt ttagcaatta tttaactgat tttcttatat
     9121 ccttgctggc tttcttgtgt agcatgataa gtttctacgt caagaatggc ctgtttaaat
     9181 ttttctcggg tgatttcata aggcacatgt ttaatatcat ttgttgccat ggctttatct
     9241 aagattgctt ctaaatcggt cgtgacattg agattcatgt cggttaacga tactggaagt
     9301 tggttctttt tcataaacac atagatttta tcaaacaaat cgaactgctg atccatcatc
     9361 aataaggtca gtacaccata actgacagat gctccgtgaa ggtatccctc cattttttct
     9421 agaacagtcg taccgtaata gaatgagtgt gccatagcag aattatagtt attgttaact
     9481 aaaacagaaa ccaatccggt agtaacaatg atatccgaaa cgacttgttc aattgcatat
     9541 gagggacgat cgtcggcact atctttcaat gcttgcgtac cataagtcaa gagacgatca
     9601 atgcacattt ttccgatttg gattcctaat ctatccgtgt agcctagatc atcgccacga
     9661 gcagaaaaag tagattcgac ttctttggat aaagcatcgc caatacctgc ccataagtat
     9721 ttatcaggtg cttcggcaat aattgctgta tcaatgaagg tgtgtacagg tggttttgaa
     9781 ggaaagaaga gtccgtccat tacgccttct tcgttataga tgacacatac cgcagtgacg
     9841 ggcgaacagt tcgaaccaat agtgggaaag gtgaagactg gtttagctag tttttcacca
     9901 atgactttta cagtatcgca agcacggcca ccaccgactg caaacaggac gtctgctttt
     9961 tggacacttt catgctggat caactgttca atatttccat aagttgaatt acccccgtac
    10021 cagagactgg cgacaacctc tatttcctgt gtcaaagcta cttttatttt gttttctgcc
    10081 tttgaaagtg cagtcttccc tccaatcagc actacttttg tcccatattc tttgacgatt
    10141 ccatttgctt tttcatacgc atctgttcca atggtataat ttggtaagaa aacactttga
    10201 ctcatattca tttccttctt tcttcgttga aacgggttaa aattttaaag agtgatcgat
    10261 ctgactgcgg ctgtagatgg agtagaggtg aacctaagac aggataagtg atgaataagg
    10321 tggcatgaac accttgaaat catcaataag tatcctaaaa acgaacaagt acaaaaacat
    10381 tgaaaacgac aataatgaat ttttgttcct cttagtattt ctaccgattc cctttgcttt
    10441 tttgtaatta tctaaaaatt ctaaaaatta agattattat atgaaaacag gttt
//
LOCUS       DAGCYL010000093        10240 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.094, whole genome shotgun sequence.
ACCESSION   DAGCYL010000093 DAGCYL010000000
VERSION     DAGCYL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.094"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            144..293
                     /locus_tag="MB216_001797"
     CDS             144..293
                     /locus_tag="MB216_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBT4526604.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            304..1818
                     /gene="dltA"
                     /locus_tag="MB216_001798"
     CDS             304..1818
                     /gene="dltA"
                     /locus_tag="MB216_001798"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBT4526605.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1815..3020
                     /gene="dltB"
                     /locus_tag="MB216_001799"
     CDS             1815..3020
                     /gene="dltB"
                     /locus_tag="MB216_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBT4526606.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3065..3298
                     /gene="dltC"
                     /locus_tag="MB216_001800"
     CDS             3065..3298
                     /gene="dltC"
                     /locus_tag="MB216_001800"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBT4526607.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3301..4569
                     /gene="dltD"
                     /locus_tag="MB216_001801"
     CDS             3301..4569
                     /gene="dltD"
                     /locus_tag="MB216_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBT4526608.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4710..5792
                     /locus_tag="MB216_001802"
     CDS             4710..5792
                     /locus_tag="MB216_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBT4526609.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5792..6595
                     /locus_tag="MB216_001803"
     CDS             5792..6595
                     /locus_tag="MB216_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBT4526610.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6665..7405
                     /locus_tag="MB216_001804"
     CDS             6665..7405
                     /locus_tag="MB216_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBT4526611.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7416..8420
                     /locus_tag="MB216_001805"
     CDS             7416..8420
                     /locus_tag="MB216_001805"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBT4526612.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8465..10162)
                     /locus_tag="MB216_001806"
     CDS             complement(8465..10162)
                     /locus_tag="MB216_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526613.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3287 a         1696 c         2077 g         3180 t
ORIGIN      
        1 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
       61 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
      121 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
      181 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
      241 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
      301 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
      361 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
      421 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
      481 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
      541 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
      601 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
      661 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
      721 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
      781 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
      841 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
      901 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
      961 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
     1021 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
     1081 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
     1141 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
     1201 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
     1261 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
     1321 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
     1381 aatcccgaaa agacagctgc tgcattcttt tgtcaaaatg acttagcaag ttaccataca
     1441 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
     1501 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
     1561 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
     1621 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
     1681 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
     1741 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
     1801 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
     1861 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
     1921 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
     1981 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
     2041 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
     2101 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
     2161 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
     2221 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
     2281 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
     2341 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
     2401 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
     2461 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
     2521 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
     2581 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
     2641 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
     2701 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
     2761 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
     2821 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
     2881 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
     2941 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
     3001 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
     3061 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
     3121 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
     3181 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
     3241 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
     3301 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
     3361 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
     3421 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
     3481 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
     3541 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
     3601 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
     3661 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
     3721 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
     3781 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
     3841 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
     3901 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
     3961 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
     4021 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
     4081 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
     4141 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
     4201 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
     4261 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
     4321 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
     4381 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
     4441 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
     4501 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
     4561 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
     4621 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
     4681 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
     4741 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
     4801 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
     4861 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
     4921 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
     4981 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
     5041 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
     5101 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
     5161 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
     5221 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
     5281 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
     5341 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
     5401 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
     5461 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
     5521 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
     5581 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
     5641 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
     5701 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
     5761 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
     5821 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
     5881 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
     5941 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
     6001 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
     6061 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
     6121 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
     6181 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
     6241 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
     6301 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
     6361 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
     6421 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
     6481 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
     6541 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
     6601 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
     6661 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
     6721 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
     6781 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
     6841 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
     6901 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
     6961 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
     7021 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
     7081 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
     7141 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
     7201 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
     7261 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
     7321 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
     7381 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
     7441 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
     7501 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
     7561 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
     7621 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
     7681 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
     7741 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
     7801 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
     7861 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
     7921 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
     7981 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
     8041 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
     8101 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
     8161 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
     8221 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
     8281 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
     8341 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
     8401 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
     8461 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
     8521 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
     8581 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
     8641 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
     8701 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
     8761 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
     8821 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
     8881 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
     8941 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
     9001 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
     9061 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
     9121 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
     9181 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
     9241 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
     9301 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
     9361 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
     9421 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
     9481 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
     9541 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
     9601 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
     9661 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
     9721 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
     9781 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
     9841 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
     9901 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
     9961 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    10021 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    10081 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    10141 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    10201 aataagcaat tcctgttaaa aataacaatg ataatataat
//
LOCUS       DAGCYL010000094        10204 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.095, whole genome shotgun sequence.
ACCESSION   DAGCYL010000094 DAGCYL010000000
VERSION     DAGCYL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.095"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..314
                     /locus_tag="MB216_001807"
     CDS             <1..314
                     /locus_tag="MB216_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526614.1"
                     /translation="FYNWNSWEKYTKNMKEIQKEFPQIVKSSPVDISGTMVGTGNSPI
                     SSYTADYYTTNQKGKMVELITQTRNTPTTATTTIWRGVTSIGENQLFYLEDLDSYVRA
                     E"
     gene            736..1473
                     /locus_tag="MB216_001808"
     CDS             736..1473
                     /locus_tag="MB216_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526615.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(1490..1906)
                     /locus_tag="MB216_001809"
     CDS             complement(1490..1906)
                     /locus_tag="MB216_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526616.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            2298..3545
                     /locus_tag="MB216_001810"
     CDS             2298..3545
                     /locus_tag="MB216_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBT4526617.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            3813..6203
                     /locus_tag="MB216_001811"
     CDS             3813..6203
                     /locus_tag="MB216_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBT4526618.1"
                     /translation="MKYEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            6252..8087
                     /locus_tag="MB216_001812"
     CDS             6252..8087
                     /locus_tag="MB216_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBT4526619.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            8156..9019
                     /locus_tag="MB216_001813"
     CDS             8156..9019
                     /locus_tag="MB216_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4526620.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            9009..10118
                     /locus_tag="MB216_001814"
     CDS             9009..10118
                     /locus_tag="MB216_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4526621.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
BASE COUNT         3803 a         1549 c         2009 g         2843 t
ORIGIN      
        1 tattttacaa ctggaattca tgggaaaaat atacgaagaa tatgaaggaa atacaaaaag
       61 aatttcctca gatagtaaag tctagtcctg ttgatatttc tggcacgatg gtaggcactg
      121 gtaatagtcc aatatctagc tatactgctg attactatac aacaaatcaa aaaggtaaaa
      181 tggttgaatt gattacacag acaagaaata ctccaacaac agcaacaaca acaatttggc
      241 gtggagtaac aagcatagga gaaaatcaat tgttttattt ggaggattta gatagctatg
      301 taagagcaga gtaaaataaa aaaagagcta acggtaccaa cgttagctct ttgtgctgac
      361 gatgataaca aaacctaccg aaaaaggatt gtttcatcac aaacacaaac agaaaagaat
      421 tgataaaaaa cccgaacctc agaattttta ttaatcaatt catttagatt ataataaatt
      481 gagctgaaca ttacaatacg tatatgttgt aaaacgtaaa aaaagtcaaa agtaaatact
      541 ttcttaaaaa gtcaaataaa actattaaag acaatatata tggtttattt gacataagaa
      601 aatggtctag atgataattg taatatagaa ttgtagactt aaataaagca taaaatacct
      661 tttatgataa ggtatagctc ttaattttaa gcagttttag atataagatt gaaagtaaag
      721 tgggggagaa taataatggc agatcataaa ttagaaaagt tattagaaga aacgtggaat
      781 ccaaaagaat ttagtgaatt ttttatggag aattttgaaa ctgacttagc agtgattgtt
      841 aaagatgctc ttagagagca agggtatcct gaaactgcaa actatataaa cattaatttt
      901 actttgtata ctgaaaataa aggaacttgg gatttttggg ctactttagc aaataaggag
      961 ttaagtgata aatcagacac aggaattcgt aacttttttg aatcaaacag agatgattat
     1021 atgtatgcta acaatcaaaa taaattgaac tttagagtag agtttgatga aacacctgaa
     1081 gaatttatag aaagacaacc gcctaaagaa aatgtagcaa aggtattgga agatcgttgg
     1141 aattcagatg aaatagtttc aacaattagc gaactaggcg gacaatatga accgctagta
     1201 gaagcggtta gagaagagct aagactgaat aagtttcctg atgttcaaaa tattgatgtt
     1261 tctcaaattg aaataaacgt caagatcacg aataaacttg attatggtag ttgggcagat
     1321 atagcattag aaaaatacat ctactcaacg ttaaaagagt ttatcgaaaa tagaatggat
     1381 atcatgtatc tacagcatcc acagtattta aactttggtg ttgaaatagc tacacctctt
     1441 gaagaatgga aaatggagca agggcttgac tagcctttgc tccatttttt tattttacaa
     1501 atgaatacag ttcgtcgttt ttaccaagaa tgattgtttg cccaactttg agattgtcag
     1561 gtgatccttc agcggtttgt aaggagaacg ttccattgaa cttatcggat accgttatag
     1621 gtagctttaa ttctgatcct tcctcatctt tgagaattgc tgtttttcgt tcatcaaaat
     1681 aaacagtctt tattaaataa tttttcgtag tgatctcagc agatttgtct ttttttgtag
     1741 tagtaccagt ttctttagaa ttagaggtat tttctacgac tattccatct ttggcatttg
     1801 agcttgtgtt tatttgattg aatattttca gcgacaagat aacgctaaga atagtcaata
     1861 tagaaacaag tgtaattaat actgataatt tccttttttt gttcattttt tcttaacatc
     1921 cttttttgtt ttgattagtt agatattcct tatctaacaa gcctttttag gctattaaat
     1981 gaagataaac cccaaaaaat attgcaatta tgacagtgca atggtactat tattatacaa
     2041 taaattttgc gaaaggggta ggtctaaatc gcaaacaaaa agaaattgaa cctagacaaa
     2101 tacaacttgt ataccgcaag ggaagcaagt ttgattttgg gtaaaggtag aaattatgtt
     2161 acaaacataa tcaactatta tccaaatcgt ttgcctaaag gttcgttcaa gaaagtgggt
     2221 gacaaaacct atattataac ggaagaagga atagaagcat tgcgatcaca aacagaaaat
     2281 aaaaacaaaa agaaggaatg aatatgaaga gaaattcgct tggtatggtt ttattggcat
     2341 cagctgtact tgcggggtta tcagtaccag taacctcaca tgctgatgaa tggactgcaa
     2401 acacaccgga tcaaatccaa attacaacag gacaatcaag ttatgcgttc aaaaaaggag
     2461 atactatttg gtcaactgta gagaagctta aaagcttagg ttacaagact actgttgctg
     2521 agtttgccga attgaacaat gttgatttgg caaaaggaga acaacggact atcccgattg
     2581 gacgaacttt caacatcagt aaagatacat taacagttaa ggaagcagat ggcgaagtag
     2641 ttagtcaaac tgttcaaaaa gataaacctg atcctattga aaatacggag caagagaaca
     2701 aaaaatccga gactacaaaa gaatcggaac caacagcaaa cacttctgat aagacagaaa
     2761 caagtgcttc aagtgttgat ggagcaagtt catctaatac aaataattct acaagtgaga
     2821 aagaagcatc acagggaaca agtgaaagta gcactacgga agctgatact ggaacatcaa
     2881 caaataactc acaatcaaca tcaaatgatg caaatgaatc aaccagtaca tcttctagtg
     2941 atcaatcaca aacaggttcg agtgatgaaa ctgacagttc tacttcacca agtaaacccg
     3001 aacaaccaac taatgttgtg ctaggcgttc catttattag tcaaggtaac acaatgcttt
     3061 gcgaaggaac atctttgcta gaagcgttac attataaagg tgttgcaaca aatcaagatt
     3121 taatgacttt tgttaaatca atgccactat ctcctgacaa taatccaaat aatggttttt
     3181 ctggtgaatg gagacacaat gtggatggaa cttatcaagg tatgaatccg gctcctgtag
     3241 tttcatgggg tgttgctaat ggcggaaatg ttgtagatat ttctggttca tcggtacaag
     3301 gattaaaaga tgagattgta aatggaaatc ctgtagtagc gtgggtaact tatgcctttg
     3361 aacctgctca acataaacca atgttttggg gcgatgctat ttggaatcga catgtagtag
     3421 ccgttgatgg atttaaagac ggtttttacc atgttgtcga tcctgtgttt ggggcaagtt
     3481 ggattaatgc aggatcattt gaaacagcat ataacgtatc tcaattagcg gtttcggtac
     3541 gttaataaaa gatatcgttt atatactaag tagttatata gtctttattt gtccttgctt
     3601 aactacttag aaaacttgtt gctcatggtg ggtagaacta aaaggggtga tgctatgaat
     3661 actagagcgt tgaaacagct tgctttcaac gaaaaaaatg attaataggt gtggtgattt
     3721 taacttagga atgggcgcga aggtcattac cacaccgaca tatcgtggct cattttcttc
     3781 gcctaactgg tataaagaag gagtaacgag agatgaagta tgaaaaaacg ataattttaa
     3841 aatcaaaaat aatttattgt tttgcgactt tagtaatgat gttatctacg cttataatgc
     3901 ctttttcagc aataagagct gaagcagatg tcacaaaacc gacaagtgca aaatttatag
     3961 aaactggcgt aaaaacagac ggttatatta gggttaatat gccgaatcat ccaaatgaat
     4021 ggatgatttc aagtcaattt aaagattctc atggaaatat tggatattgt atggattcag
     4081 aattgccatc cccaacagga agtggagccg gaagcttgaa gtacaaggga gctggcagtg
     4141 atgagttcta tcgtatgttt aaaggtggtt ttccatcaaa aacagcaaag gaactgggtg
     4201 caggaaatga tacagaagca tggtatgcaa cacaattagt ttcatgggtt ttagccggaa
     4261 actttaaagt ttcacagatt gtatggtcac atccaaacca taccgccgca gaaacagctc
     4321 gtgtaaaaaa agcatttgaa aaaatttatg actatgctaa gaatggaaaa gatacgccca
     4381 acactgagtt ttctatcaca gcttctaaga cagctgacga aggaaaatat cacactttca
     4441 catataaaac agcatcaaat aagactggta atgcgaagct cacttttaca tcagcaaaac
     4501 cagctggtat gaagatttat gatgcagatg gtaaagaaat tactaacaac actgtgaaat
     4561 taaattcaag ttttactatt aaagtacctg tgactacacc aagtggtact ttgtcattta
     4621 aaggtactgc taatgttagc acaaccaatc cttttacttt tgatggacga ggtgtgtatc
     4681 aagatgccgt agttatgatc actacttcag aaacaaagga ctctaaatct ttgagtgcta
     4741 aatggacaag agctgttggt caggtaacag ttaagaaagt tgatcaagat ggaaatgctt
     4801 taaaaggagc ggagtttact ctaactgaca ataacaatgc taaatctgtt aagacaaccg
     4861 gagatgatgg gttagctacg tttgatttgc aacaaggctt aacatacagc ttagaagaaa
     4921 caaaagttcc tgaaggttat caaggttcat ttaaaaagga tggtattact gttaataccg
     4981 atggacaaga atttagttac actgctacga acacaattaa aaaaggtaaa gtaaaagtta
     5041 aaaaggttga ccaagatgga aaaccattag ctaatgctga atttaccttg acggatgatt
     5101 catcccaaac acaagcagaa aaaacagatg aaaacggact tgcaagcttc gatattgtag
     5161 caaacaagac ctacagcctt gaagagaccg gagtaccaac tggttataca ggcgtattca
     5221 agcaagaagg aattaccgtt aaagatgatg gacaaacttt tgaatataca gctacaaaca
     5281 ctttgaaaaa aggtttagta aaagttcata aagtcgatca agatggaaaa cctttggcaa
     5341 atgcagagtt cactttaacc gatgaaaaag gtgcaacaca agtagaaaaa acagatgaaa
     5401 atggacttgc aagcttcaat attgtagcaa acaagacgta tagcctagaa gaaaccggag
     5461 taccagtagg ttacacaggt gcatttaagc aagagggtat caccgttaaa gacgatgggc
     5521 aaacttttga atatacagct acaaataatt tgaaaaaagg tgtagtaaaa gttcataaag
     5581 tcgatcaaga tggaaaacct ttagcagatg cagaattcac cttaacggat gaaaaaggtg
     5641 taaaagaagt taaaaagacg gatgcaactg gtctggctac gttcaacatt attgcgaaca
     5701 aaacttataa cttagaagaa acaaaagtac caacaggtta cacaggcgca ttcaaacaag
     5761 aaggaattac cgtcaaagat gatggacaag tctttgaata tactgctaaa aacactttga
     5821 aaaaaggtgt gatcaaagtt cataaagtgg atgatcaagg gaaagcatta gcaaacgcag
     5881 aattcacttt gacagacgac aaaggtgtaa aagttgtaca aaaaacggac agcaatggct
     5941 tggcaacatt taatattgtt gccaacaaaa cgtacaaaat cgaagaaaca caaaacccta
     6001 atggttatac agggaaatgg tctaaagaag gcatcaccgt tgctgacgat ggacaaacct
     6061 ttgaatatac agtaattaat caccttgtac ctgaaacagt aagaacaggc ggaatcggta
     6121 catcagcctt ccttcttgta tttctaattg caggattgag tatggtgcta ctgtattatc
     6181 gagaacagca caaagcaaaa taaaaaagtg ttaatggaaa agaagtaaat atgaaaaaag
     6241 aaggctgacg tatgaaatca aaaaaaattt tttcaaattt aatgctattt ggtactttgc
     6301 taggagtggg attgggcgca agctcaatct cttctagcct tggactaggg gggcaagtag
     6361 catatgcaca acaaaactat aattatcaag gaactggaac gattcatatc cataaagtaa
     6421 caaatactaa tggggatggg aaccctgtct cagatattga taacaaaggg aaagaaatag
     6481 ctttaccaaa tggtgtaaaa cctcttgaag gcgttgagtt ctcttatgtg aaacttacta
     6541 aagatgaagc taaatccttg ggatggacgg atagtgatac tacaaaaggt caacatattg
     6601 atgatgctaa agcacgatta ttactaaaca aaaaatacag tgaagtaagt agaatgaaga
     6661 ctgattcaca agggaatata gacataccta tacaagtaaa tacagacgat ttaacagata
     6721 acttgtattt gattgttgaa acaggaacag tcaaaggtgt agttgaagaa gcgtatccta
     6781 tgattgttaa cgtacctgaa ctaaacggat cagatgatca atactttaat tatgatgtac
     6841 atgtctatcc taaaaactat ttccaattaa actcagcaga gcttgataaa aaagacgaag
     6901 atggaaaggc tcttgccgga actgtttttg aattgcgaaa agatgatgga tctgatcagg
     6961 ttgttggaac ttataaaaca gacagcacag gtaaattaac tgtagatggt ttggaattag
     7021 gtgactatta tttcctagaa accgatttgg gaacagatgc taataaaaca acctatttac
     7081 tagatggtaa aaaattcaaa tttagcgtta aacgtgggga gactgctaaa gtagttcttg
     7141 atgttaccaa ctacaaacga cctgtaatta ctaaggtaat tgataaagaa accgttgata
     7201 ctggcgaaga tgttcaatat acattgaaac catcaattcc tggtaatatt ggtaattacg
     7261 aaaaatatgt tgtcaaagat gttctaggtg cagagttaac ttatgattca tcagaagtga
     7321 tcgaaggaat gactttagat actgattaca gtatcgaacg tgacggacaa acgacaaact
     7381 atgtctttac tgaatcaggt atggataagc ttcagaaaca atttgatgca ggggatcgct
     7441 tctttacaat tttgatgaac gctcatacta atagtaaagc taaaatggat acaccactta
     7501 agaatgatgt ttcaacgatc tacaacaatg gatatgaacc cgaagacgaa acaccagttg
     7561 atcacgctta tacaatcatt ggtgatcacc aattcattaa aacagatgga gatacaagca
     7621 aagcattgag tggagcaaaa tttgtagtga agaacaaaat tgatccgaat aaagttactt
     7681 acctaaaaca agatccaaca acgaaatctg ttacttggat tgcagataaa tctaaagcta
     7741 ctgaattcgt ttccggatca gatggtaagt tccaaattga aggcttgcaa tatacctatg
     7801 atgtagattt tgataaacaa ggtaatccaa caatcacaga taaaaaaatc aatgattatg
     7861 ctcttgaaga agttgttgct ccttcaggat atacaccaac tagaaatgat gttccatttg
     7921 tggtatcgaa tgatgatgct tctagcgaag taacagacat caagaacctt aaaacacctg
     7981 aaacagttcg tacaggtgga attggaacag tagtatttct agtggttgca ggtgtggcag
     8041 gaacagggct atatgttgtc tacaaacgag aacaacacaa agcttaatga aataaaattc
     8101 cctagagtgt ttttggctct agggtttctt tttaaaaagt gtgtgaaaag taggtatgag
     8161 catgaaaaag aagaatatca agttgatcat cttaaaaatt gggatgttgc tatgtttagg
     8221 agtaggtcta tatcctgtaa ttactcaggc ttatacctac taccaaactc aacaaacact
     8281 aaactcctat aaggaaaaat atgatgaagc ttctgaaaag gagaatgaag aagtcttcaa
     8341 gagatattca gaagttaata ggaaacgaaa agacacagaa caaacctcag tcacgattcc
     8401 tgaagataat aacaatattc cgtcaaatgg aaatcttcag aaggataaat taggtgaatt
     8461 agtgggtgaa attcttatac caaagattaa tgtggatttg ccaatttatg atggtgtaaa
     8521 cgatttccaa ctatcagcag gagcaggaat attaaccgga actgattatc caactggtgg
     8581 gattggaaac cattctgtta tatcagctca ttcaggcatt cctaataaaa aatattttac
     8641 agatttagag ttgctcaaac ttggtgataa attttttatt gatatttcag ggaaaaaact
     8701 tgcttatcaa gtagatcgga taaaaacaat tctaccaacc gaatttgagg atttgcttcc
     8761 tgtggcagga aaagattatg taacacttat gacatgtaca cctgttggag ataattctca
     8821 taggttactc gttagagggg ttagcatacc ttaccaaaaa agtattgttg agaaagaaaa
     8881 gaagaaagaa gatttaaagg taaaggcgca gatgaactgg tatcctatcg tagttgtagg
     8941 ggttttatta ataatagctg tgatagtcat tattaaaaaa atagtagcaa agagacgagg
     9001 aatgaaaaat gaagaataaa atactacttt ttttgactct tatttgcact ttggttttaa
     9061 caatttatcc tgcaaatcag ctctatcatc aattcaaaac aaaagagaat gtggcggaaa
     9121 tccaagagaa aattgagaaa aaacaaactc cttcaaaaga ggaagtggca aaaaaagtcg
     9181 aacaaataaa atcgaacagc ggtaaccaag gattagacaa tactcagaaa acagataaag
     9241 gagtagacaa tactcaggaa acagacggtg gatcatttga tattcgtgaa gctgaaggaa
     9301 aggtcaatcc atacacagga gatgtttttt caaaaaatca aacgaaagat tttgaaccat
     9361 ctacctattc aggagcggtt ggttatgtct atattccaaa aataaatgtg ctagagcttt
     9421 tgtttctagg cgcttcacag gagcatttat atagaggtgt ggcacaaatg agtgctacta
     9481 gtctaccttt atcaggtaaa ggtcatcaaa cagtaatagc aggtcatcgt ggcggttata
     9541 cgtcaccaca atttctttat gttgatcaat tagtagccgg agaccacatt tatatagatt
     9601 atcttggtga aaaagcagtt tatacggttt tccgaacaga cgttattaga aattacggtc
     9661 agaattcaac actgactgat attcctgatt tagatatgtt aacactttat acttgtcatc
     9721 catatcctca gaactacgat cgtttattag ttagaagtct aagagatgaa aaagcaaaat
     9781 acatcacgcc acaagtaaca gttggcaata ccgcatacgc tgatgccatt gatagcgagt
     9841 ccgtttcaga taagacagaa actgttacat cttctaatga tttatttgaa gatgttgatc
     9901 gtgccaacaa ggaattagat ggaaataatg atcttaatat tccggaacaa ggtagcgtag
     9961 agatgcaaga cagaacgttt gctgaaaagg caagttcaat atttacagga attaccaata
    10021 aacatcagaa aatgctttta gtcatttgga ctgtaatagg agcattagca agtctaaagg
    10081 ttcttattct tttaatcaaa gagtttcgaa aagcataatg aagattataa aaattgatag
    10141 agtacagtaa aaagggattg atactattat tgatctcttt tttattttga atagagctag
    10201 attc
//
LOCUS       DAGCYL010000095        10191 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.096, whole genome shotgun sequence.
ACCESSION   DAGCYL010000095 DAGCYL010000000
VERSION     DAGCYL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.096"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            98..373
                     /gene="rpsP"
                     /locus_tag="MB216_001815"
     CDS             98..373
                     /gene="rpsP"
                     /locus_tag="MB216_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBT4526622.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            385..636
                     /locus_tag="MB216_001816"
     CDS             385..636
                     /locus_tag="MB216_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBT4526623.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            761..1552
                     /locus_tag="MB216_001817"
     CDS             761..1552
                     /locus_tag="MB216_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526624.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1722..2243
                     /gene="rimM"
                     /locus_tag="MB216_001818"
     CDS             1722..2243
                     /gene="rimM"
                     /locus_tag="MB216_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBT4526625.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2233..2994
                     /gene="trmD"
                     /locus_tag="MB216_001819"
     CDS             2233..2994
                     /gene="trmD"
                     /locus_tag="MB216_001819"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBT4526626.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3130..3477
                     /gene="rplS"
                     /locus_tag="MB216_001820"
     CDS             3130..3477
                     /gene="rplS"
                     /locus_tag="MB216_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBT4526627.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            3735..4028
                     /locus_tag="MB216_001821"
     CDS             3735..4028
                     /locus_tag="MB216_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBT4526628.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            4871..7198
                     /locus_tag="MB216_001822"
     CDS             4871..7198
                     /locus_tag="MB216_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBT4526629.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            7324..7653
                     /locus_tag="MB216_001823"
     CDS             7324..7653
                     /locus_tag="MB216_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBT4526630.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            7693..8697
                     /locus_tag="MB216_001824"
     CDS             7693..8697
                     /locus_tag="MB216_001824"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBT4526631.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            8694..10123
                     /locus_tag="MB216_001825"
                     /pseudo
     CDS             8694..10123
                     /locus_tag="MB216_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
BASE COUNT         3624 a         1850 c         2000 g         2717 t
ORIGIN      
        1 gaatttaatc aagctgtaaa gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta
       61 catcttgtga ataaaacaat ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa
      121 acgtatgggt tctaaaaaga gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc
      181 tcgtgatgga cgtttcatcg aaactgtagg tacttacaat cctttgaaag accctgcaga
      241 agtagtttta aaagaagact tagttcttga ctggttatca aaaggtgcac aaccttctga
      301 cactgtacgt aacatccttt caaaagaagg cgttatgcaa aaacaccacg aagctaaatt
      361 ctcaaagaaa taaggtgacc ggatatgaaa gatttaagcg acttagtctt aacaatcgtt
      421 cgtccgctag tcacttatcc tgatcaagtc aagttagacg tcgtagagtc gaaagacttt
      481 tacgagtaca atctaacagt ggctcctgaa gatattggcc gtatcattgg aaaacaaggg
      541 cgtgttgcta aagcaatccg cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa
      601 gtgcgtttga atatcatcga tgataaggaa aagtaaataa acgtgctgta ctcgaatttt
      661 cgagtgcagc ttttttgttt tttttattaa ttgaagaaat gattatttgg caaaacaagc
      721 aagaaagcgt atccttatag gaggagggag tgaacagcca atgagaaaga aaatgttatt
      781 tattccattt gctctattgt taagcgcttg tagttcggtt actcaagaat cagacaatac
      841 acatctatca agcagtatat ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac
      901 ggcttccacc actgaacaaa caatggctac ctcaaataat gagaaaaaga ctgctttaga
      961 ccagttgaaa gaacaacagc caaatgttcc catgcctcta gatgtaccag tctcttcagg
     1021 ctatctcaat atcgcagcga cacatacaaa gcaaggttat tctattttat attacagaac
     1081 agatcgtccc ctcgggttga atgctgacga attgaatcaa gaaacgccta ttgcaaccta
     1141 tctatatcaa tatgggtttg cttctagtca agaaacaatc caagttttgc agccatttga
     1201 gatcgacaca aatggtcagc aagtggactt aggttctcga atcacagggt atcagcaagg
     1261 tgcagcaggt tcaagttttc ttgaatggca agaaggaaac tggcgtattc gaatccgtgg
     1321 gaataatata gaaggccaag atcccttact attagctaaa gaaatcgtag cttatttgga
     1381 agaaaacagt ctaccagcac ctgaacaatt tggtaaaatt actgttgata tgggtgatac
     1441 gacgaatcga gccgtagaag taagctggca agaaccgaaa aatgcctata cgatcactca
     1501 tcaagatcct atgtctgcgt tgaagatggc agtttctatg aagaggttgt gaaatcagcg
     1561 cgctgatctg agcccatcat ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc
     1621 gcatttttgc ataattttgg cttaatacct taaaagaaaa ctagaattgc ctcttcacac
     1681 atgctaaaat aaaacagaca aaaaagaaat ggggatataa gttgactgaa tacttgaatg
     1741 taggaaaaat cgtaaacaca caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg
     1801 atttcccaga agaacgttac aaaaaaggaa cggtgctaac actattccaa gacggaaaag
     1861 cacctgtgga attaacagtc aaaagtcatc gcaaacataa aaattttgac ttattgagtt
     1921 ttgaaggaca tccttctatc aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt
     1981 caaaagacaa ctctgtagat ctagctgaaa atgaattcta ttaccatgag atcatcggtc
     2041 tgcatgtatt agaagatgga actgagttag gtaaagtaaa agaaatcttg tcaccaggtg
     2101 ccaatgatgt ttgggtcgtt caacgtccga aaaaagctga tatattgctt ccatatatcg
     2161 cttctgtcgt caaagaagtc gatttagaag ctggagtggt ccgtgtagaa attccagaag
     2221 gactgattga cgatgaagat tgatgtattg actttatttc caagaatgtt tgaaggtcct
     2281 atgggggaat ccatcatcgg taaggctgta gataaaggat tattagatat caatatctct
     2341 aacttccgcg attattcaga taacaaacat cagacagtag atgattatcc atacggcggt
     2401 ggagcaggta tgctgctcaa agtccagccc atctatgata acatccaagc cattgaaaaa
     2461 gctgcaccgg atgtcaaaaa aagagtaatt cttcttgacc cagctggtaa acgattcgat
     2521 cagaaaatgg cagaagaatt ttcacaagaa gaacacttag tattcatttg tggtcattat
     2581 gagggatatg atgaacgtat ccgcacatta gtgacagatg aagtttcctt aggagattat
     2641 gtccttactg ggggagaatt aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg
     2701 ccagatgtgt tagggaatca gacttcagct caaacggatt cttattcaac cggtttattg
     2761 gaacatcccc aatatactcg cccggcagaa tataaaggga tgaaagtacc agaagtattg
     2821 acaaatggaa atcataaatt gattgaagaa tggcaattaa aagaatcact aagaaggacc
     2881 tatcaacgtc ggcctgatct tttggaaaca cttgaatgga cacctcaaat gacgaaattt
     2941 ttagaagaaa tcaagaaaga agaacaagaa aaaacagcag atgaagccaa gtgacttttt
     3001 ctaaaatctt ctttacagta ggttgataat atgttagtat tttatggtga gtgcaaaagc
     3061 acttaactat tacgatattc cgctgtgaga aaagctcata agaatatttg gaataaggag
     3121 aagaaaacca tgaatccatt aatcgaagaa ttaacaaaag aacaattacg ttctgacatc
     3181 ccagcgttcc gccctggtga cactgtgcgt gttcacgcaa aagttgttga aggtactcgt
     3241 gaacgtatcc agttatttga aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa
     3301 acttatacag tacgtaaagt ttctaacgga gtgggcgttg aacgtacatt cccattgcat
     3361 acaccacgtg ttgcaaaaat cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg
     3421 tactacctac gtgcattaca cggaaaagct gctcgtatca aagaaatccg tcgttaatca
     3481 ttgttatcag actgagaaag aacccttggt attcaagggt tcttttttct tagcatgtaa
     3541 tttgaacacc ctgatccagt tttttcttta attattggta ggggagaata atggattaga
     3601 ataaatctac ctataagtag tagtaaaaaa aagagcacta tgataatatc tttcttaaga
     3661 gtaaccgctt taataaaaaa tccagaatat tctaacataa atggagtatt cttagaatca
     3721 aaaggagaaa gaagatggtt tatacttata cggagcaaga aaagatgaaa gttttagaca
     3781 cattcatgaa tgatggaaaa gtaaaacaag taccaagttc aacaaaaaag aaatacatcc
     3841 tattgaaaga agtcttgaaa cgctttgacc atggagtgac ttatacagaa acagaggtta
     3901 attcgatact gctgaacgta ttttctagtg gcgattatgt cgaacaaaga cgttatctaa
     3961 tcacatttgg ctttttcaaa agaagttcag acggtcgggc atatcagatg atgggaattg
     4021 aaaattagaa gttttaattt atactcattg aaaaaagttc aagtatgcga tattggttct
     4081 acaccgcata cttgaacttt ttttgtcatc tgtatctata atacaaagcc aagaacgtta
     4141 ttcaaaatat ggatagctat gataaaagct aatgagcgat tcgcagttaa ataagcaatc
     4201 gtcagtaaaa tcgttccagg agcgtacatg aaaaaatcaa ttggatgcgt tacatgaata
     4261 aagataattc tcttttattt gtacgaataa ataccaaaac gtacctccta aaattagatt
     4321 ttggacagat gccctttaaa aagataacaa gatttcgttc ttgaaacaat atatatgctt
     4381 ttattccaat gaggttgtga aaacagtatt ttgtcctgag cgttagaaaa ttgaaaatgg
     4441 ggaatagcaa ctcgtacttt accatttttt aagtgaatac aagatcagct aattgaacac
     4501 cgtttattcg tgatgaggga cacaacactg cagtcgacac atttctcttt tatgttcaaa
     4561 gataacaaat atttaaaaca aagaaaaata gtagataatc ccgtcctaac tcttttattc
     4621 ttccctcata gcttccaaat ttaagcaata cttcaattaa tcaagtttta tctttttttt
     4681 tcaataatcg taaaaaaaga gtatttttta tttaaaaata aaatctttgt attaaaggac
     4741 aagactaaaa taaccttaga ataaaaagag aaaaaaagcc ccaaactttc tatacatcaa
     4801 taaaataata aaaattaaat ttagaataac gtgtaaagga ttatttgatg attttttatt
     4861 gagaggaggg atgaaaatga ttaagaaaac tcaaaataaa atccaacgta tatgtcaaaa
     4921 acattttgtt tctagactca aagctgtatc taaaagaaaa tacttacatc ccattatgat
     4981 ttcgatcatc ttgattttta ctttcttagg gagcttatca agtatttatg taaacgttta
     5041 tacagaaacg tcaaattttg ctaaccaaac gacagaggag acaaatagtc caccctattc
     5101 tgaaatacag tccatagaaa gcaccaatca gtctagcaac aacgagacaa cctcaaccac
     5161 atcctcgact caactgattg atccgacaga aaaagaaatc acagcagcaa aagcagcagg
     5221 ggagaaagct tattcagaaa caggtagaag ccagacattt agtactgttg cggccactgc
     5281 gaatttcaac ggacatacca taccagatgg atatacagtg atatatgcca aagatggagt
     5341 cattgttgcg gttaacggat cgatgagtgc ggataaaact gcgttacaag gcgtaaccaa
     5401 agcaatcgct tttattgata accgtccaga tatccataat ctgtttattg gctccacagt
     5461 aaatggtact actatcacat tcccaaaaag tatctatatg ggggtggcta ccgctggaaa
     5521 tacgatcgat tcagttacac ccaatgcgga taaaagttat tcttttacac cctcaacatg
     5581 gggagggctt cctgccggag gaggtatgag tactttttca atcggtatac agttagcaaa
     5641 tgatattcca ttcacggggt actcgatcaa aaatgggcag atcgttgatg gtaagggaaa
     5701 tgtttcaaat tttccaacct atcaagcagc aacggttcag gtcaatgcga atcggacagg
     5761 aactttttct tatacagtca atggtggagc agcaaaaatc ggaacgtacg ggactacatt
     5821 atctggaatc catgtgggtg acaaagtggt tatcactcca acagcagtaa cgggctattc
     5881 ttttgcacaa acaaacggtt ctttcacagc cctagaaagt aacgtcgata cggtgaccta
     5941 tacaggcaaa caaacaccaa tcactattaa gtatcaagac tattttggac agatgattgc
     6001 tccttctaaa ataatgaatc ttacttacgg aagtgctgca caagacctga cgactaatac
     6061 gcctactatc aacggatata cctttacagc tgtttcagcg acagaaacaa agaaccaatc
     6121 agcaatgagt gttttagcta gtttagatac aaatggaaat gtaatcgtaa aggacgcaaa
     6181 tggaaaacag atttctgaag tcatcctata ttataagaca aatgcaacag tatcaatcaa
     6241 tgcaaatggc agtaagtact atgatggtct ttctgttgta ccgatcgtta aatatgcctt
     6301 taatgataaa acagaatcaa catcactttt aaacaatgat ctcagtattg atcaaatcac
     6361 atggaatcca actgatttca aagcgatgaa tgaaaaggga gttgaagtaa ctgcgcctac
     6421 tgaaattgga acctatacgt cttggcaatt gacaaaaagc ggactagcta aactagctgc
     6481 cagaacaaac tatttattta ctatcgtgca gacatctgat gtctatacga ttaagcaaat
     6541 cagcggtgcg gtagagctca gtggcagtaa gacatatgat ggaaaagcag gtgtcccgtc
     6601 aatccatgtc aagctagcag agggtgtcac tagttcgctt actccagtac aagtggcact
     6661 tagcagtatg gattacacag cagatgcaca atcagcaaaa aatatggtta atgctggcag
     6721 ctatacaatt aaactaacga atagcggaat agacaaagtc aaagcagcaa atcccaagtt
     6781 gtcatttact gatatagcca acacatcagg aacttatacc atctataaag ctaacgcagt
     6841 gattactgtc gatgacgctg cctttaatta tgatgcccaa agtcactcga tttcggttgg
     6901 aaatctccac gttaccggtg ttgtcttagg cgaatcattg gattataccc taacagttaa
     6961 cagccggaca gatattggat cacaaatagt aacgattagt ttaggcgaag gaagtgtaaa
     7021 caataattat acgattatcg caacagatac cgctaagttg acgatcaatc cgactctctt
     7081 gcttccttcg acaggtggga taggtctact gccctttgtc tttttaggac tgatctttat
     7141 tttttctggc ttcttttatt ttatccatcg taagaaaaaa gcaggtgaac agcgatgaaa
     7201 aaaacaaaaa atgtacgtgt ggatatattt gctgttctct tttttatcct actggtcttt
     7261 tttataggtt ctccgactgt gtcagctaga gaatcaccta ttacatttac tttgcataat
     7321 cgaatgttcg aaagcgataa gatgccaagt atcagtcaaa acgacggaat aaatatcccg
     7381 gataactttc aaggagtaaa agggttgaac ggcgttactt tcgctatata tgatgtctcg
     7441 gatgaatttt acaagctgcg gtcagaagga agtagtgtgg aagacgcaca aagaaaacta
     7501 gcacaaaaaa gtgatagtga aaaaatactg gcagaaaatg tgacaaaaac agtagacgag
     7561 gaagagggca ttgcttcttt tagtgcatcg gacaaagatg agcaaggtcg tgacgctgtt
     7621 taccgatttg cggaaataaa gacttcagat taagtaaaag aaaaatcagc accatttgtc
     7681 gttgtgcttc ctgtgacaga ttcatctaat cataaattga cgaccatcca cttgtatcca
     7741 aagagcgaac aaaaaatacc tgctgcactc acattaacaa aaacagtaga gaataagcag
     7801 acagattttg cagatgggga taaagttcct tatctcatca ccgtaacaat tcctgagaac
     7861 atcaacgaga taggaaccta tacgatcaaa gatacagctg atcctcagtt atggctagaa
     7921 ccagaaacga tcgatttttt gattggtgga gacaagattc atacatttca cacacaaaaa
     7981 acaaaacatg gttttgtctt atcagattct ggaaaagatc ttagttcgtt tgctgggaaa
     8041 aaattaacga tccgttacca aatgacattg aaagaaaaca gtgaaaaaac gacatttgat
     8101 aacgatgtac gtttgacgac tgacaatcaa acagtagaaa cacatcttgc catccaaaca
     8161 ggaggcaaac aatttgtcaa agtggatcgg caaacgaaac aaaaactagc agatgctcaa
     8221 tttctagtca aaaaaaatga ccaatactta gcgcaagaaa acgggataaa tcattgggta
     8281 gccaaagatg attcaagagc aacgattttt acttccagta aagatggaac attttctgtc
     8341 catggattgt cttttggaag ttacgaacta gtggagatta aaccgcctaa aggatatata
     8401 ttgagtcaat caaccattct ctttgaagtt gaaaaaggca gttatgatcc gcttcatcct
     8461 ataccattgg aaatcatcaa cgaaccaaaa gcaaatccga cgaataatgg aaaaacgcca
     8521 gttcagatgc agacaaatgg tccatcaaat gaaaaaaatg gaggaagctt tccaaaaaca
     8581 agcgaagaga tgcttggagg tttctcaatt ttagggttgt tgctagtcct ttcaacaggg
     8641 acagcttggt tttataaaaa acaacaaaaa ggaaacaata ggagggaaat aaaatgaaaa
     8701 acatcaaaac aaaaatatgg acgtttttag gaactgctat catgctgctt cctttcgttc
     8761 tcggcctagg aactgcagag gtttctaggc cgtatcacct acaccagaaa atgttacggt
     8821 taatttgcat aaattgaaat tcacttcagc accagaaaat caaataaaca acggaacgga
     8881 attgacattt ccgaatagcg aaccgttgaa cggcgttgaa ttcaacgtat atgacatcac
     8941 tgctacgtac tatccaagta aagacacagc agttccagca gacgctacac ccttcgcaag
     9001 tgtaacgact tctggtgaag gattagctaa cctgactctt ccaggtaaat ctgatgggaa
     9061 agatgctgta tacgtctttg tagaaacacc aaagcccggt gtcgaaacgt cgccaaatat
     9121 cgtgttaagc ttaccctttt ataacccgaa ttcatcagac cctgacaaac ctttagacac
     9181 gattcaccta tatgctaaaa atgtcatcaa agaaagcaat atcaaaatcc agaaagctgg
     9241 gaacttttca ggggtagata agttagcagg ggcgcaattc cagatcaaaa attcagagaa
     9301 tcaattcgtg accggttttg atgaaaacac tggtgtcgct ctctatgatc ctgctgagtc
     9361 agcgttgaca ttcacaactg atactgaagg atccatcagt gtcaatggat taatagacgg
     9421 caattatact ttagttgaaa caaaagcacc agatggttat caattaacca atccacaaaa
     9481 agaaactact tttatagtaa aaaacggaaa attgcaacaa acaaccggta tcatccatgg
     9541 cgcagatgaa gccaatcttg ggaatccaaa cggtgtagtc aatcgggaaa atacaactac
     9601 ggataacacg attactgtag aaaatgtcca aaactacggt gatttccact tcatcaaaca
     9661 agatgtgaac acgcaagaaa aattagccga tgcagaattt tctattctaa gagcaaaaga
     9721 tccaactgtt aaattaatga aaaaaatagc aaatcttgcc tcaggagaat acccatatgc
     9781 ttggtcagat gaagtcacag gttctgactg ggaagaagtg aaattgattt ctgattcaga
     9841 agggaaattt tcaatcgaag ggctgaaaca aggggcatac tgccttcaag aaacaaaagc
     9901 accagaaggc tacgcattac ctaagaatga agctgcgtac actgaattta cagtagatga
     9961 tgatccaaca acttctgatg aaacaacgat cgataaccaa cctaaaggaa tcttgccttc
    10021 aactggtggt atggggatca tcgtattcat cttggtaggt actgctttag ttggaggagc
    10081 agtgatctac ttcaaaaaac gccatcaaga aacagaagct taaactattg gaaacaaaaa
    10141 tcatacgata aataaacgat agtgattcaa atggggagaa aggttgtgac a
//
LOCUS       DAGCYL010000096        10132 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.097, whole genome shotgun sequence.
ACCESSION   DAGCYL010000096 DAGCYL010000000
VERSION     DAGCYL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.097"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            116..622
                     /locus_tag="MB216_001826"
     CDS             116..622
                     /locus_tag="MB216_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBT4526632.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            639..1538
                     /locus_tag="MB216_001827"
     CDS             639..1538
                     /locus_tag="MB216_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBT4526633.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1625..2509
                     /locus_tag="MB216_001828"
     CDS             1625..2509
                     /locus_tag="MB216_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBT4526634.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2701..2772)
                     /locus_tag="MB216_001829"
     tRNA            complement(2701..2772)
                     /locus_tag="MB216_001829"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2737..2739),aa:Arg,seq:ccg)
     gene            2832..4166
                     /gene="rpoN"
                     /locus_tag="MB216_001830"
     CDS             2832..4166
                     /gene="rpoN"
                     /locus_tag="MB216_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBT4526635.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4315..5406
                     /locus_tag="MB216_001831"
     CDS             4315..5406
                     /locus_tag="MB216_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBT4526636.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5447..6448
                     /gene="gap"
                     /locus_tag="MB216_001832"
     CDS             5447..6448
                     /gene="gap"
                     /locus_tag="MB216_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBT4526637.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6558..7748
                     /locus_tag="MB216_001833"
     CDS             6558..7748
                     /locus_tag="MB216_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBT4526638.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7852..8607
                     /locus_tag="MB216_001834"
     CDS             7852..8607
                     /locus_tag="MB216_001834"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBT4526639.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8723..10021
                     /gene="eno"
                     /locus_tag="MB216_001835"
     CDS             8723..10021
                     /gene="eno"
                     /locus_tag="MB216_001835"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBT4526640.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHHLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3548 a         1870 c         2100 g         2614 t
ORIGIN      
        1 gtcacacctt cttttttgta tataaaaatc ccaagtaaat acataaaaaa taaaaaaatt
       61 gaaactttta gcgcaccttg tacgtcatat taagtgaggc agaaaggagt ggagaatgat
      121 ttttactgga tataagaaaa aacggcagat cgaaaaatac cgtcacctgc tgaatggact
      181 catcgaaact gattatcaaa aaatgttccg tatcacgatg aattacgtac atgataaaga
      241 agaggcctta gatgtgatgc aagatagttt tcataaagcc ctgaactatt ttgaaaaaga
      301 aaaagagatc gagcatttta gtgcatggtt ttatcggata ttgatccgca cggcattaga
      361 tagttggcgc agacaaaaaa ggacgcctgt ggacttattt gaaattcaag atatccccca
      421 acagttacct ttagaaactt ccgttgcaga gctccatatg attttagata aaatagacag
      481 tccggaaaaa gagattctga tcctctactt ttttgaagga tttcgtttga aagaaattgc
      541 gactatcttg gatatgaatg aaaacacagt taaaacgaaa atgtaccgct cattgaatgc
      601 tttacgtcaa atcctagaat gaaggagaga agagtgccat gtattcccaa aaagaaaatt
      661 taaaaaaact aaagaaaact tataaagaac aagcaattcc caaagggctt aaagaggaga
      721 tccgccagcg ttttattatc gaagagcaat catttataaa agcaaagcgg agaaagagac
      781 gtctccaagg gttcggagta tctttggcta tcgtaatgat tacaacaagt agccttcttt
      841 ttaatagtca agttcgaagc tttgcagaag atttacccat cttaggatca gtcattgaat
      901 tgatcttagg cgaacgcttt actgatcgat ctgaaaaaat cgatattcag gtaccgaaga
      961 tcaatacaca aaatgagaaa gaaaataaaa caattcatgg attaaatcaa aaatactttc
     1021 gcgaaggaca agcagatttt gaaaaagctg aaaaagaata tggaaatttt gaaacggatc
     1081 attaccaagt tttgggagat tatcagaaaa tcgtagacga taaccggttt ctcgtgattg
     1141 aaaggcagat cacccaaaca gcagcagata gccatgttga aaaaagatac gacacaatcg
     1201 acaagaaaaa cagtgtccag ctctcacttc cacttttatt caaagatgac acctatctat
     1261 ctgttttgac aaaagaagtc aaacggcaga tggccgaaca agtcaaggaa gatccatcta
     1321 agtactattg gactgagcaa gatattcaag aagggacgat agaaaaacca acacttgtta
     1381 cgcctacacg aagtttttat ttgaacaaag agcatcaatt agttttgact ttttctcaat
     1441 acgaaattgc tcctggttat atgggtacac cagaatttgt cattcctaag tccgtgacaa
     1501 aaacgatttt ggcatcggaa gattatttgg atcattagac gagaagtagt tgcgactagc
     1561 agtttcaatt ttcaagtaag tacaagtaaa taataagaag agaaaaagag aaagaagaga
     1621 tatagtgaaa agaaattatt tagttatcag tattattgct ttatccgtgc ttttgttttt
     1681 cgaaattact tcggtggcct ccttgatcac cgaagtaaaa ggacaaacac actcttcgga
     1741 gcattcgtca caatctttca aacaatcaga acaatcaacc attaaaagcc aatcaactga
     1801 aacagctaac gtcaaagaag ccattcaaga atcagcggaa aaggtacaaa gtcagtcatc
     1861 acaatcaaca caagacagta caaaaacgag ccgagatccg gctgtcaaaa gcgtcgcaat
     1921 cagctttgat gatggaccag gagcgaccac aacaccgcag cttctgcgaa tcttaaaaga
     1981 aaaaaacgtg catgccactt ttttcgtctt aggagaaaat acggcgcagc atcctgaaat
     2041 cgtgaagcag acagctgaag cagggcacga gattggcaat cacacatacg atcatcagaa
     2101 tctggccact ctttctgccc agtcgatgac ggaggaagtg acgaaagcag acactgagat
     2161 caaaaaagtc actggcaaaa cccccacttt tgttcgtccg ccctatggtt ctgtcacgaa
     2221 tgtaggagca acgatcatcc agcgaccgat catcgaatgg tctgttgatt cagaagattg
     2281 gaagacaaga aatcctgatt tgattttgca gaagatccaa gcaacagtat acgatggggc
     2341 aattatttta ttccatgata tctatcctga aacgattcgt gccgttcctc aagtgatcga
     2401 ttatctgaaa gagcaagggt atcgcatcac gacagttggg gatttattag ggcatccaac
     2461 ggctgtcgag aactattatg gcagaaacga tcatcggcca gttcagtaag tgagacaagt
     2521 atctttaata aataacacat tttaataagt agttgaacac ataactacct tacttgcaaa
     2581 ggcttaacaa tatctataca ggtacaacaa aaaactgtcc accaattttc tttggcggac
     2641 agttttcact taaaaataaa gaagcaacta ttcgtaaatt atgctaaaat agcagtagaa
     2701 cgcgtcctga gggaatcgaa cccccgtctc aagaaccgga atcttacgtg atatccacta
     2761 cactaaggac gcaagcactg ggataattgt aactaaacag ataaaaaatt acaaggggat
     2821 tggacaaaaa aatgaaattt gagcaaaatt tttcacaaaa acagcagcag acacagaaac
     2881 tagccatgac gcaacagttg caacagtcca tccaagtttt acaatataac agtgaggagc
     2941 tgttggcatt cgttgagaat caagcaatgg aaaatccatt ggtggaagtt gttgagccgg
     3001 aatggcagcc agattacata aaagcttctt catcttctta tgaaggagaa gagacaaatt
     3061 acctaaatca gattcctgat acaaaagggt ctttatttga ttcattgatc gaacaagtcc
     3121 atttgaatta ccgagatact tttttgagga aaatcgttct ttatcttgta gaatacatcg
     3181 atttgaacgg atttttgacg attagtttag aagaagccgg aaaagagatt ggagctacgc
     3241 ctatccaaat gctcgatgcg ttgactctga ttcagcaatt agaaccagct ggagtaggtg
     3301 cacgtagttt gcaagaatgt ctcatgcttc aaacagaacg agatgattat gcgccggaat
     3361 tagcttatat cgtgttggaa gaatgttttg aggaactggt ggagcgaaaa tggaaagaaa
     3421 tcgcacaacg tttttctgtt gatctacatg ctgttcagca gatattcgac tatttacaaa
     3481 cgttgagtcc aagccctggc cggattttcg accgtagcag cgaattgtac attattcctg
     3541 atgtccgggt attagtggac gacgataaaa atgtacaagt tatctcaaac cgaaaaaatc
     3601 agccaaatat acgctttcaa gagagttact tcaaacaaat gtcacaacaa gcagataaag
     3661 aaactgaaaa ttatttaaaa gaacggaaac aggaattcga atggttgaag aaaacgatcc
     3721 tacaacgtgg ggatacaatt ttgcgggttg ctcaagtcat tgtttcccgg caaaaagctt
     3781 tctttatcga caaggaacgt ccaatcaagc ctcttacgtt aaaggaagtt gcaactgaga
     3841 tagatgtgca tgaatccacg gtcagtcgtg cagtcaacgg gaaatatttg gaaacagact
     3901 tcggtgtatt tgaattaaaa aaatttttca ctacacgaat tcctacgaat agtacagaac
     3961 agacagaaga cctttcagca gatacagcga agaaaaagct gcaggaatta gtcgatcaag
     4021 aagacaaaaa caagccgttg tctgaccaga aattggttga attgctgaaa aaagatgaga
     4081 tagcgatctc aagacgaaca gtagcaaaat atcgtgattt attaggaatc ccgagttcat
     4141 caaaaagaaa acgttatgat aactaatcag tcaaaagact agaaaaagta tttcactttt
     4201 tctagtcttt ttagttgaaa aacgagagca aaactgttag acttacagtc gtgaggttga
     4261 gatttccctt tattgatgtg aaattcgaat agttaatttc tttttttatc gtatatgggt
     4321 cgttttaagt ctacattgga cgtaaagtgt ccaactaagg agatcgctat gctggatgaa
     4381 ataaaaatga ttgaatctgt tgcaccagat attatcgaag tgttgcaaga acgctataaa
     4441 attttacgaa atatctattg gatgcagccg attggacgtc gaagtttatc ggaaagtatg
     4501 gggatcacag aacgagtatt gcgaacagaa acagatcttt tgaaaaatct gaatttgatc
     4561 gacacctcaa aaagcggtat gacgctgaca actaagggcg aagaagttta tcaaagcctt
     4621 gaaaacttca tggatcagct tttaggtacg catcaaaccg aacaacagtt ggcagaatat
     4681 tttggtaacc aacgctgtat cgttgtttca ggaaacagcg aagagcagac caaagtggct
     4741 gatgcattcg gcgaagcgct gagcgaagcc ctagaccgac tacttccaga aggcgagaat
     4801 atcatcgctg tgatgggagg aactactatg gctgtggtag ccgaacaatt gtctaatctg
     4861 gaaaataaga aacggcataa cctgtttgtc cctgcaagag gcgggattgg tgaagcgata
     4921 actgtacagg caaattctgt cagtgcaaga atggctgcta aagcaaacgg taaccaccgt
     4981 gcactttatg ttccggaaca attaagtctg gcaacttaca attctttact caatgagcca
     5041 tctatccaag aagtcttaaa cttgattagt gaagcgaatt gtgttattca tagtattggg
     5101 cgtgcattac acatggcagc acgtcgtaag atgactgaaa aagaattagt catgctgaaa
     5161 caagcaaatg ctgttgcgga atcttttggc tactttttca atgaaaaagg tgaagtcgtt
     5221 tataaggtcc ctagaatcgg catagaattg caagatttgg aaaaagtccc gatcatcatg
     5281 gcaatcgcgg gcggaaaatc aaaagctaaa gcaattcgag cttatatgaa aaatgcacca
     5341 aaacaaacgt ggctcatcac tgatgaagct gctgcaaatg agattttaaa aggggtaacc
     5401 ctttaaaata aatatttttt tgattttcat aaggaggaaa tcttaaatga cagttaaagt
     5461 aggtattaat ggttttggac gtattggacg tctagcattc cgtcgtatcc aagatgtaga
     5521 tggaatcgaa gtagtagcaa tcaacgactt gacagatgct aaaatgttgg cacacttgtt
     5581 aaaatacgac acaactcaag gacgtttcaa cggaacagtt gaagttcatg aaggttcatt
     5641 caacgtaaac ggaaaagaag ttaaagtatt agctaaccgc aacccagaag aattaccatg
     5701 gggcgaacta ggcgtagata tcgttctaga atgtactggt ttcttcactt ctaaatcagc
     5761 agctgaaaaa catttaacag ctggtgctaa acgcgttgtt atctctgctc ctggcggaaa
     5821 cgatgttcca acaatcgttt ataacacaaa ccacgaaaca ttaactggta aagaaacagt
     5881 tatctcaggt gcttcatgta ctactaactg tttagctcct atggctaaaa cattgaacga
     5941 caaatttggt gttgttgaag gattaatgac aactatccac gcttacacag gtgaccaaat
     6001 gactctagac ggacctcatc ctaaaggtga cttccgccgt gcacgcgctg ctgctgcaaa
     6061 catcgttcct aactcaacag gtgctgctaa agctatcggt ttagtaattc ctgaattgaa
     6121 cggtaaatta gacggagctg ctcaacgtgt tcctgtacca acaggttcat taacagaatt
     6181 agtaacagtt cttgaaaaag aagtaactgt tgacgaaatc aatgcagcaa tgaaagaagc
     6241 ttcaaacgaa tcttacggat acaacacaga cgaaatcgtt tcttcagata tcgttggtat
     6301 gacttacggt tcattatttg atgctacaca aactaaagta atgacagttg gcgacaaaca
     6361 attagttaaa actgttgctt ggtacgacaa cgaaatgtca tacactgcac aattagtacg
     6421 tactttagaa tacttcgcta acttataaga tagacaaacg tacaagtgta aattatagac
     6481 atgtacattt aaaagcgggg aagcaatcgc gcttcttccg ctttttttga taataagttt
     6541 attcaggagg aaataaaatg gctaagaaaa cagtgaaaga tatcgattta aaagacaaaa
     6601 aagttcttgt ccgtgttgac ttcaacgttc ctttgaaaga cggcgtgatc actgacgaca
     6661 ctcgtatcaa agcagcattg ccaacaatca actacgttct agaacaaggc ggaaaagcaa
     6721 tccttttctc tcaccttgga cgtgtaaaaa cagaagaaga taaagaagga aaatctttag
     6781 ctccagttgc aaaacgtcta ggcgaattat taggtaaaga agtaacattc gtacctgaaa
     6841 ctcgcggaga acaattagaa gaagcaatcc gcaacatgaa tgacggcgac gttgtcgtat
     6901 tcgaaaatac acgatttgaa gatattgacg gtaaaaaaga aagcaaaaat gatacagaac
     6961 taggtaaata ctgggcttca ttaggcgatg tatttgttaa cgatgctttc ggtacagctc
     7021 accgtgcaca tgcttctaac gtaggtatcg cttcaactgg tatcccaaca gttgctggat
     7081 tcttgatgga aaaagaaatc aagttcatcg gtgaagcagt agaagaacca aaacgaccaa
     7141 tgattgcaat ccttggtggc gcaaaagttt ctgataaaat cggcgtaatc gaaaacttga
     7201 ttcctaaagc agataaaatc ttgatcggtg gcggaatgac ttacacattc tacgaagcaa
     7261 aaggaatcaa gatcggtaac tctctagtag aagctgataa agtggaacta gcaaaagaat
     7321 taatcgaaaa agcaggcgac aaacttgtct tgccaatcga caatgtctgt gcaccagaat
     7381 tttcaaatga tgttgaaaca caagtaatcg aaggagacat tcctgatggt ttgatggctt
     7441 tagacatcgg cccagcatca gtaaaattat ttgctgatac attacaaggt gcaaaaacag
     7501 ttgtttggaa cggacctatg ggtgtatttg aaatgagtaa ctttgctaaa ggtacaatcg
     7561 gtgtttgtga agcaatcgct aacttagaag gcgcaacaac aatcatcggt ggcggagact
     7621 cagctgcagc agcagaacaa ttaggatttg ctgacaaatt cacacacatc tcaacaggtg
     7681 gtggcgcaag cttggaattg cttgaaggta aagaattacc aggactagca gcaatcaacg
     7741 ataaataaac gaccaagttt gctcaattct gacgagtaat cgatcaaaag gtgaagggag
     7801 ttttcatgac tccctagcca tttttcaata aattaaggag tgtgttcctc aatgcgtaaa
     7861 cctattatcg caggtaactg gaaaatgaac aaaactgcat cagaagcaaa agaatttgct
     7921 gaagcagtaa aaaacaaaat cccttcaaat gacaaagttg attcagtcat cggatctcct
     7981 gcattattct tacaagaatt aatgacatca gctgaaggta cagaactaaa aatctcagca
     8041 caaaactgct actgggaaaa ctcaggtgcg ttcactggtg aaacttcacc agcagctctt
     8101 gctgatctag gtgttcatta tgtgatcatc ggacattcag aacgtcgtga atatttccat
     8161 gaaacagacg aagacatcaa taaaaaagca aaagctatct ttgcaaatgg tatgacacca
     8221 atcctatgct gcggcgaatc tttagaaacc tatgaagcag gcaaaactgc tgaatggatc
     8281 gaaggacaaa ttacagctgg tttagctgat ctttcagctg aacaagtgtc aaacatggtt
     8341 attgcttatg aaccaatctg ggcaatcggt actggtaaat cagctgacgc acaaatcgct
     8401 gacgaaattt gtggagttgt acgtcaaaca gttgaaaaac tttatggaaa agaagtatca
     8461 gaagcagttc gtatccaata cggcggttct gtaaaaccag aaaatattgc tgaatacatg
     8521 gcaaaagaaa atgttgacgg tgctttagtc ggtggcgcaa gcttgcaagc agattcattt
     8581 ttgtcattgt tagacgctgt aaaataattg ttgaaaattt gcgaaaaatg caagaaatgt
     8641 ttgcactaat gagcaaaatt aactaaaatc gtatatgagg gaaatccctt aggaaaaaac
     8701 aaactcaaag gagagacaaa acatgtcaat tattactgat gtttacgctc gcgagatctt
     8761 agactcacgt ggtaacccaa caatcgaagt agaagtatac acagaaagcg gagcttttgg
     8821 ccgcggaatg gttccttcag gtgcttcaac tggtgaatac gaagcagttg aattacgtga
     8881 cggagacaaa gctcgttatg gcggtaaagg tgtaactaaa gctgtcgaca acgtaaacaa
     8941 catcattgct gaagcaatca ttggctacga tgttcgtgac caaatggcta tcgacaaagc
     9001 tatgatcgct ttagacggaa ctcctaacaa aggtaaatta ggtgcgaacg ctattttagg
     9061 tgtttctatc gctgttgcac gtgcagctgc tgactaccta gaagtacctt tataccacca
     9121 cctaggcgga ttcaacacaa aagtattgcc aacaccaatg atgaacatca tcaacggcgg
     9181 atcacatgct gataactcaa tcgacttcca agaattcatg atcatgcctg ttggcgctcc
     9241 aacattcaaa gaagctctac gtatgggtgc tgaagtattc cacgcattag cttcaatctt
     9301 gaaagctcgc ggattagcaa cttctgttgg tgacgaaggt ggatttgctc caaaccttgg
     9361 ttcaaacgaa gaaggttttg aagtaatcat cgaagcaatc gaaaaagctg gctatgtacc
     9421 tggtaaagac gttgttcttg ctatggatgc tgcttcttca gaattctacg acaaagaaaa
     9481 aggtgtttac gtactagctg actcaggcga aggcgaaaaa acaactgacg aaatgatcaa
     9541 attctacgaa gaattagttt ctaaatatcc aatcatctca atcgaagatg gattagacga
     9601 aaacgactgg gacggattca agaaattaac tgacgtatta ggcgacaaag ttcaattagt
     9661 tggtgacgac ttgttcgtaa caaacactca aaaattatct gaaggtatcg aaaaaggaat
     9721 tgctaactca atccttatca aagtgaacca aatcggtaca ttaacagaaa ctttcgaagc
     9781 tatcgaaatg gctaaagaag ctggctacac agcagttgta tctcaccgtt ctggtgaaac
     9841 agaagattca acaatctctg atatcgctgt tgcaacaaat gctggccaaa tcaaaactgg
     9901 ttctctatca cgtactgacc gtattgctaa atacaaccaa ttactacgta tcgaagacca
     9961 acttggtgaa gttgcagaat acaaaggttt gaaatctttc tacaacttaa aaaacaaata
    10021 attcttttct aatgaagata catgatcgac tattgaaaga tagtcgagta tgatcactta
    10081 gttaagtaat cgaactctct acttgctgaa atatcagcag gtagagagtt tt
//
LOCUS       DAGCYL010000097        10126 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.098, whole genome shotgun sequence.
ACCESSION   DAGCYL010000097 DAGCYL010000000
VERSION     DAGCYL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.098"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(74..271)
                     /locus_tag="MB216_001836"
     CDS             complement(74..271)
                     /locus_tag="MB216_001836"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBT4526641.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(417..1295)
                     /locus_tag="MB216_001837"
     CDS             complement(417..1295)
                     /locus_tag="MB216_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBT4526642.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1334..2452)
                     /locus_tag="MB216_001838"
     CDS             complement(1334..2452)
                     /locus_tag="MB216_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBT4526643.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2449..4548)
                     /locus_tag="MB216_001839"
     CDS             complement(2449..4548)
                     /locus_tag="MB216_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBT4526644.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4599..5420)
                     /locus_tag="MB216_001840"
     CDS             complement(4599..5420)
                     /locus_tag="MB216_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBT4526645.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5417..6226)
                     /locus_tag="MB216_001841"
     CDS             complement(5417..6226)
                     /locus_tag="MB216_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4526646.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6247..6741)
                     /locus_tag="MB216_001842"
     CDS             complement(6247..6741)
                     /locus_tag="MB216_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4526647.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6753..7241)
                     /locus_tag="MB216_001843"
     CDS             complement(6753..7241)
                     /locus_tag="MB216_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4526648.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7318..10014)
                     /locus_tag="MB216_001844"
     CDS             complement(7318..10014)
                     /locus_tag="MB216_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBT4526649.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3077 a         2276 c         1661 g         3112 t
ORIGIN      
        1 tcttgctatc tttttataat cacaactctc tataatagaa ttacgattta ttttatacgg
       61 ctgaaaaaat ctatcaccaa aaaaattcaa acattaagcc aaggattatg aagtcaacat
      121 ttcgaaaaat gaacatacat tggtaagcaa ctataactat taccacagac ataaagtaac
      181 acaatatatt actatttaag tgtacgccac cagtgaatat cctcaaggta tacagcgcac
      241 aacaaaaaga aaaaaggaaa cgcagaagca tttcctttca gtgatttaat gaaaacaaca
      301 cgatttgact tagagccttt ttatttgaat tcattttcgc tttatccaac ttcatttgat
      361 catttgatta ctcggttaaa tacttttgac atgagtatca atgaaacatt cattaattat
      421 ggttgcttat ttcttaccgc ttttctggcc aatgctaatc cgccgattat tccggcatta
      481 tcacctaatt cgggcgtaat aatgtagcta tcaattggtg gtatttcaac atatccattt
      541 ataagctcaa caaatttatc ctgaactttt ttcttcatgt gttcttgttt cattacacca
      601 ccgccaaaaa taattctatc tggcgaaaac atcagtgttg tattgtatgc acattgggca
      661 aggtaaaagg cttcaatctg ccaaaacgaa tcgtcagcca ataaattctg gccttttaca
      721 cccaaccgtt tttcaatcgc tggtcctgct gccatcccct ctaaacaatc ttggtggaag
      781 ggacagtttc ctgaaaaatt gtcctttgga tgtcgcttga tcaacatatg ccccatttcc
      841 ggatgactga acccttccac gaaagtccca ttgaacaagg caccacctcc gatacctgtt
      901 cccacagtga agtatgcaac acctgctaac cctttgccag aaccagcaac atattcacca
      961 tagcaagccg cattgacatc tgttgtccat tcaatcggaa tattgaaata ttgctttagc
     1021 gttcctacaa aattgaaatt ttgccaagca agttttggtg ttgatgtgat atgaccgaaa
     1081 ctcgttgatt tgcattgaat atcaattgga ccaaagcagc cgaccccgat gcctgccaat
     1141 tgttccttaa aaggtttaaa aaaatcaatg acctgcttca ttgtttcaac tggagttgtt
     1201 gtgggaaaac tgactctttc caaaatcgtt aattcctcag taccgactgc acagacaaat
     1261 ttagtccctc cagcttcaat tgccccatat atcatcatag gttcctccct tcgaatatgt
     1321 gaaaaacact tcattaacgc ggcaagcgtt ctcgatcagt gaaatgacgt ttagtatttc
     1381 ctgaagcttc tgttgaatac cagtaagcga cactagcaac atcgtcttgc cgctcaaata
     1441 agcgaacatc atcgttgccg atttgttgta aagtgaccga taaatcttcg gtaaaacaga
     1501 tggggtcttg aagatgccaa cgataaagac catgtcgtgg cattgcgtcg tcaccaaagg
     1561 catgaacggc attcaatttt cccgtttcaa aacgatctct tgtggcatct ctatttgatt
     1621 ggtaaggata ccctaaaaag gctgttgaat agtttttcgc atgtggacgt ccgaactcat
     1681 cgtactcatg aaaggcccac gccccgccaa aataatcttc tgaacctgtt gaggttacgg
     1741 tcggatactc actatcccca tcaatgtaga atttaaactc gccttcgccc caccaataac
     1801 gttctaggca ggtaagcgca aggtaagtac ccacgtaata gccttgaccc tttaatcgat
     1861 caattaactt ataatcctcc tgtaatttgg taatgcgttc ccgatgccaa tttgcgtgaa
     1921 aatataggct attttcttcc aattcgtcca ccaaagcgta atttatcgta taaaaaaatg
     1981 aggacatatc cttcggatgt aaattctcga tcgtaatttt agcggatgtt tggaaaggca
     2041 tttcgaaata ggaattaaag ccccctgtag gattaaccac tatcggcaac gagttcacct
     2101 cgcaacgctc tccaaaccca ttacaaaaga aatcccctaa aggactttca acagcagggc
     2161 tatccgaccc atcccaataa attcttaaga ttaaatcacg taaaacaaaa ctgccaaatt
     2221 ctgttcgatc acgaatcgtc atccacatat gacgaattat tcccgttcca gtaatatccg
     2281 caatcacagt ttcttcacca gaagtcagcg gaattgcgcc acgacctttg cggcttggtc
     2341 ctagctcgct tggcaacatc gcagctttac ctttctcgcc tgttggattt tcgggagtga
     2401 ttgctcgact ctggattttt ttaaattggg taatattttt tagtatactc atctttttct
     2461 cctttgaatg tgaatcgttt ttcccttttc tacgtgaaga atttttttcg tatcacttat
     2521 tttcaatggc ttttctgaca aatttgaaat actcaactcg gattgcgtac attcaatcga
     2581 aatgaaggct tgaccgaact tcaaattctt ccaagaaaac gttaaattat caacaggatc
     2641 aatttgcaat actccttttt tgaatttcaa tccgaaatat tgactgatca ttaggctgac
     2701 aaaaagaccc gatgcccagc cgcatttgcc gcagccaaag tctcgcacga cttctccttg
     2761 ttgagcctct aagcgatacg gccaccacca ccacgaaccg tctaaatctg ccaaacaaat
     2821 tagttcttct aatcgggtta cgaaagcttg agggttgtga agctcgctca ttaaaacagt
     2881 gataaagcca ggaaaagtgg cgccggattc taatccccaa gtgatgcctt tgatttcctc
     2941 gctataggtt ggattgaaag aactagcaga aaaggccatg gtgttctgat ataaatgatc
     3001 tttattatct aaaaattgat aaaaaggcat caacgtagtg tctgattctt ctccatcatg
     3061 catcaataaa ttaatttttc catccacaat cacatcactt aaaaagatta acccttgctc
     3121 aagaatcggt ttttgatagt gcttcactga ataggttgtt ttctcctcat caccgattcc
     3181 ctccaaaaat tgctcaccaa aaggaccatc gatagtcatt tgtttacaaa ttgattcctt
     3241 tagtttacag gcaatgtttt tgtatcgctt gctaagactc agttgttcta gggcttcaaa
     3301 aatttctgca agtccaatac aggccttcca aagacaaaga ttgctgccgg tatgatactt
     3361 tccaagtgca aaggcgtctg ataaccaaac agatggaaaa agcattggtt cccctgaccg
     3421 acgttgagct aatatgtcct caataattct tgaagcattc agtagaacct gcggacaccg
     3481 ctcaaagaat tttgtatccc cagtatgttc aaaataaaga ctagccagta acgcactgga
     3541 taacgaatta ctcagagaat gattgatacc tcctgtaaat tttgaaccag gaaacttaac
     3601 gccatattga tcaaaccata gaaccgtttt ttgacataac tccggttcaa acatggtgaa
     3661 tggcaatgat gcgtaataca tatccttatt ccaaattcga ttagtagcag gatagctgcc
     3721 ccaattgctg ccgacgacct gtccctgtcg attcataccg aaactagaaa agctttgata
     3781 aagtgcacga cgatagagat ggacaactct atcgtttggc attgacagct gcccaaatag
     3841 ctgagcaaaa tagttcaatg tttccttcag ccatttttcg atatcagatt cctgaaaagt
     3901 cattacttct tcaaaagcat ttgggtccac aaaagcaatg gcgaagcttg ccacttcctg
     3961 ttgtgcgatc gtgtccttat ttcctttttg agaaatcaac acattttgct gatccgaata
     4021 cttctcttga tacgaaggca catctttcac tgaaacttga aggctttcgt gtgtttcatt
     4081 cacaatgtaa acctgttgaa caagcatact taaacgctta ttagccagta tgggagcaaa
     4141 ggatactaca atgaccttaa acgtcgaata atgatgaata tatctaggca aacagtcttg
     4201 aatcacatcg acttcaatac gatgatccga ttctgacaaa tggtagacaa tcccgtttac
     4261 ttcaaccttt aaggataacg aattggtttg agagagctgt ttattgaccc aaacacctgg
     4321 tttttcctca gttagtaaat tttctcgata aaaggtcaac gtttgaatcg tcccgcaaac
     4381 atcaaggttt gctaatgtct tttgattact tacatcgaag gtcaatccct ttacatcagg
     4441 aatattccga tagtagctgt attcgtttga agtagtcaca tttccgtcgt ttaactcagc
     4501 attaaaaaat gggtgtttgg gtgaataaat tacttcatta atagtcatct tttccctcct
     4561 tgctaaaaaa actgcagcct agtagctgca gcttcctact atgcgattcc aagtaatgac
     4621 aataaaattc ccaagacgac cattccaaaa ataattttga tgacgccgac ttttttatct
     4681 agtaatttga aacagctcaa tgttaccaat aacggtacaa ttcctttaaa aatactatcg
     4741 aggatttctt ggacattcat tagttcttcg cccttcattg aaaagctcaa aacggtattg
     4801 aatttgacca tactgatcgt cattgcacct accatgatca gacccaagat actagcagct
     4861 tttgtcagaa tttcaattaa cccgttcgca tacagctttt caatatattt agagcctaat
     4921 gaatagccta aaacgccgcc atagtatcgc atcaagtaat taggtatatt gaacataact
     4981 aaaaagataa ttggtgccaa aatatttccg gcattggcta agttgatcgc tagtcccgcg
     5041 gtaacgacac gccaaactcc ccagaacaat gaatcgccaa ttccagccaa tggtcccatc
     5101 aaactcgttt tgatcgcgtt gatactttcc acgtcaaaac cagctttttc agaattttcc
     5161 ttctccatcg atgccgcaat ccccatgaca aaggacgcgc aaggaatggt cgtcgaaaaa
     5221 tactgtgtat gacgttccag cgccgcagct cgatcttctt cacttttgta gaaatgatta
     5281 atgaagggca tcatagaata ggcgaaacca ccgccgccca tcgtcaccgg attcaccgaa
     5341 gcatacatag tcatcgagcg ccaaaacatc ttccgaacca attttttctc atctttagaa
     5401 attccatcag ctaatttcat tgaaagaaat cctcctcttc ttggtcaagc gttgcattta
     5461 cagcagcatc gctttgaact gcatgaacag atatattttt tagttgtaga tctctttggg
     5521 ctataacaat tgcaataatc gttccaactg cggcaattgc aatcaatggt aactcgagat
     5581 acgccataac aacggcccca aagaaaaacc acacagcaat atttttactc caaagcatcc
     5641 gtaacagcat cgccatccca actgccggca gcagatttcc agcgactgtt aatccgttca
     5701 tgatgacctc tggaatatta tccaagacgc ttttgacaac atctgctcca aataaaacgg
     5761 caaagaatgg aataagagca taaagcgcat agtggacaaa ccacataaag aagtgcagat
     5821 tgatgattcc tttttgatca ccagattttg caaatttgaa aaatttagca gccatcggtc
     5881 cacatacaaa agaaaaaatc atattcatta actgtaatcc taatactgct agtggaaaga
     5941 caatgggaat aatcgcatcc acgtttcctc ccattttgat tgcgaacgct gtagccagcc
     6001 cagcagccaa agaaggctcc gccgtactag agattccgat attgacgaca cccataaaga
     6061 tggcttctag tgacgccccg acaatcaacc ccgtctgcaa atcccccatc aacgctcctg
     6121 ctaacggagc aacaacaatc ggacgactca gcatggacat cccgaacatt tcatgtccag
     6181 caactccaat aaaaacggcc aaagcgacta caaatgcatc atacataatt tttctccttc
     6241 ctttttctaa aaatcactca aaatagagat agctgaacgt ttttccatcg ccggtgtagg
     6301 ctgaaaaacg gtatctggat aaagttcaac taattctttt aagtcttgaa tttctgacgt
     6361 cgttaacatc acttcctttg ataaggtttt cttatcttcc gatggcggtg tcattttccc
     6421 agcattcccc acgttcatta attcgatttc cgttgggaaa gctttcgcaa cttttaccgc
     6481 atctttgaca ttagggacta aaaccatcaa gcgcatgtcc ttcgcccgcg gatcttttaa
     6541 caaatgaatc gcgtcatcaa ctgttttaat aatgactttg acattcgatg gtgcagccat
     6601 cttcaatgtc atcttttgaa tatcgttggt agccgcctca tcgcttgcta ctactaaccc
     6661 ggttaaattt agactctttg tccaagtcat cgctacttgt ccatgaatta atcgttcatc
     6721 aattcgcact gctttaatca tttttcttcc tcctaaaaaa aatcttcctc ttcatttact
     6781 acatgtacct catcaagaat ggtcatcttt aattcctgtt ggcatttatt taccagttcc
     6841 tgaatatcct cctttgttaa ttccttggtt gccaatacta tttcgagcag aattggcaga
     6901 ttaaagccag acaaaatagt tacagcaatc tctttctcca aaaaggtttt ggtaatcttc
     6961 tgattgacac ttccgccaaa taaatcggta aaaacaaaaa tctgatcctg ctcgttcacc
     7021 tggtttacaa atacattcaa ctcctgttca aaatcacact catccacata agcattaatc
     7081 accgtaatct cttgaacctt ccccgtcagt aattgaatac tgctaagaaa gcctgaagct
     7141 aagtgaccgt gagtcgctat tagaattttc ttcatatggt taattccttt ctttctgttc
     7201 acttttatat aagcaattcc tgtgccaact tttaaaaaca ttggtttaat cacgttaatt
     7261 cacattaaaa aaagtgtcta acctaaaaaa aacagattag acactttttt agacacttta
     7321 tttttgaata tacggtccta ataattcata gagcagcgaa agctcataag cattgacttt
     7381 aaaattatat ttcatctcca ttgattgaaa aatacttcta gaaacataat aaaattcatc
     7441 ttcatctggt ttcaaatcat aattcggttc ttcagatttt tctgtccgag ccaagaataa
     7501 acgttccatc atcaatgccg tatgcatata gagatttagt tttacacggc catttaaagt
     7561 gaaatgatag taattttcat atttagaaat gactgtttcc acttcactca tcacgatcgt
     7621 tgggttcaga aagttcaaac ggtccgcaat tccttccaac gaaaagaatt ttactagctc
     7681 ctgaatcaag cggttaaatt ctgccggctt catttcagga ctcaaaatat gctccaaatt
     7741 ggctgctcca tcaccgtcta agatgtcata aacattaata ttaggaattg aaaaggaacg
     7801 tggaagatcc gaagtcgtga tgaccaattt tgtcttgtca aaataagttt catcattttg
     7861 gtcgattaat tcacgtagtt ctctgtagtc cttcgtgaaa acttcgatgg attcatccga
     7921 taaatgatga cgaaaaacat ctttcaattt gtccgaaatc cccaaacccg acatacagga
     7981 aatgataatg ttgctacttt gagaaaagcc ttcaaagtat tgaacattga ttgaatactt
     8041 gtctgctgca tcctccgcaa tctgtttaaa tggcgcattc atcgtcatct tcaagccaat
     8101 atcaagtgca atggaagtcg tcaaattatt cagaagaagt aactcccctt ttaaatcgtt
     8161 cttgatctgt gtgtaaattt gatttaaaga gcccatatct acgatcagca ctagactatc
     8221 tgtattcact tgccgtttca cgaaattcct tgtcgattcg acaatctctg acacattact
     8281 ctctactggc atatccaacg cttcaaaaac ataattgccg cataattgat tcactactgc
     8341 ttgaatgctg ctagccgtac tatctccgtg agcaagcaac aggcccttca gctcgatgga
     8401 ttcatctaca taatcagata aagtcacgac aataagcagt cttagaaagt tctcgtcctt
     8461 ttctgagagc ggtggtaaat gctccaagaa ctgattacat aagtaagtcg tccgaggtag
     8521 atttgcagct aatgtacgat ttatcccggt taagtctgtc ttatagcttg gggaaatgac
     8581 ataggatctg ctaataattt ctgcaacagg tccactcgtc gtcaatccgt acgtttttcc
     8641 taaaacggat acccagacct ttctaaacat ctgaatcatc aattttctac tcattgataa
     8701 aaaagaatcc ttatcaatgt gatgcaccaa atgctgtact tcattcttta tcgaagaaaa
     8761 attttcattg aaattacctt catttatcag ctgagtaatt ttttccaaaa tcgaatcata
     8821 atcaaacggt ttgattcgat ccgtttttat cttttgattt ttctttatca ataaactagc
     8881 aatcggcaag ctttctactt cggattccag catatttaac tgaatcaaga tctcattttt
     8941 cccaatcgtt aatggttctt cttgtgttcg actatacaca tccgcacaac tgattttaat
     9001 gatattcttc agcttaccaa tattcccact aaagggatga gataacatta aagacaaggc
     9061 ttctcttgaa acaataatat ctttttgtaa aacatccgct tcattttgaa aaaataattg
     9121 aatcagctct aatcgttctc gaatggggcg atcttcaaaa gttggcaaca taacactaat
     9181 ttgaatacgc cgatcaaagg tgtctaataa ataattttca cctttttccg tcgtggcgaa
     9241 aataaaacga accttagcag aatgccaatt tacattttca ccgactggac gatagtttcc
     9301 agtatcaata aatagaaaga gcttttcttg attttctttc gataggcgat ggacctcatc
     9361 gagaaaaaga tagccgttgt tggccgttgc caataaacca tcggtatcct tttcagcccc
     9421 cgtaaaggcc cccttcttgt atccaaacag tgttgccgaa agcagttcgg gattgtccgc
     9481 ataatcggca cagtttaaga tagcaaatgg tgcatctttt tcaataattt gctcatggat
     9541 agcgtattca aaaatttttg tggctaaaaa actttttcca acacccgtgg ctccatttat
     9601 taatacactg agtccgttag gcggatattt gacagcggct atacattttt gaatgacctc
     9661 tctcagactt ccgtcatgac caactacaga atgaaaggct tcgtttattt gctccgtatt
     9721 atctacaacc gcatattcat ctaaaggctt ccacaaaaca ggctttcccg gtaatttctc
     9781 gaccttttta tcttccacta atctcgtcaa ataatggctg gcattctgac gagagatacc
     9841 tagattcttc gaaattttct cggcagtgac agaagacata ttccctgtta gtcgtatcag
     9901 ctcatcaaaa actcgatctt tagcgctctt tttcattttt tcctccagtt taatgtgtct
     9961 aaaagcataa taaactatcg gctgcaatat gtctattcta tatttgtatt ccaattttta
    10021 ctttgaaaca caaatagtcg aatatttgcc tcagatatct gacaaaataa tttatctacc
    10081 aatttagttt ttatctaata tgatttttat gttttttcat agctac
//
LOCUS       DAGCYL010000098         9979 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.099, whole genome shotgun sequence.
ACCESSION   DAGCYL010000098 DAGCYL010000000
VERSION     DAGCYL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.099"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            507..713
                     /locus_tag="MB216_001845"
     CDS             507..713
                     /locus_tag="MB216_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBT4526650.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            713..937
                     /locus_tag="MB216_001846"
     CDS             713..937
                     /locus_tag="MB216_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526651.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            952..1389
                     /locus_tag="MB216_001847"
     CDS             952..1389
                     /locus_tag="MB216_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526652.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            1382..2089
                     /locus_tag="MB216_001848"
     CDS             1382..2089
                     /locus_tag="MB216_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526653.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            2470..2649
                     /locus_tag="MB216_001849"
     CDS             2470..2649
                     /locus_tag="MB216_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526654.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            2742..2927
                     /locus_tag="MB216_001850"
     CDS             2742..2927
                     /locus_tag="MB216_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBT4526655.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            2961..3392
                     /locus_tag="MB216_001851"
     CDS             2961..3392
                     /locus_tag="MB216_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBT4526656.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
     gene            4329..5603
                     /locus_tag="MB216_001852"
     CDS             4329..5603
                     /locus_tag="MB216_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4526657.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            5657..7084
                     /locus_tag="MB216_001853"
     CDS             5657..7084
                     /locus_tag="MB216_001853"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBT4526658.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            7097..7969
                     /locus_tag="MB216_001854"
     CDS             7097..7969
                     /locus_tag="MB216_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBT4526659.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            8064..9059
                     /locus_tag="MB216_001855"
     CDS             8064..9059
                     /locus_tag="MB216_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4526660.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
     gene            complement(9664..9771)
                     /locus_tag="MB216_001856"
                     /pseudo
     CDS             complement(9664..9771)
                     /locus_tag="MB216_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3494 a         1475 c         1939 g         3071 t
ORIGIN      
        1 gttattgcta gcatgtatgt tagaaatccc gatcatttta cacgatcgat gggcatataa
       61 ataaaagaag agttgtagtc ctaaatgagg attgcagctc tttttgtttt acatagaaac
      121 gggaacagga aaaggcctgt ttaggaaggc acaagcgcct tctcaattaa aaaacctttc
      181 tctcactcca accgccaccg ccgttcgaga agaaaggaag tgaacgctgc acgttcacta
      241 agatatggtg tcttgcaaag caagacaaaa taggaaaagc ttcctctcac caaaaggttc
      301 gttcctgttc gctatctctt tattgatttg ccagaccggc aacaagttgc cttttatcac
      361 caaaaagcga caaggacaag ggtaaatggc acgctgcgcg cccttgtcct tgtcgctttt
      421 cgttgattca ttctggccaa caaatcaata aagaaagaac taacgaaagg aagtaaaacc
      481 tttcctaaga ggaggcagaa tcaatcatgc gaaagaaatt tgattattgg ggtgttccct
      541 tcagcgaatt attcccaaac tatcatgcac cacatactgt cgaatgcgat tgtggagaaa
      601 gagcgaaatg tatcaaaagc tatcgtttgt atcaatgtcc aacatgcggg aaaaaatata
      661 ccttgtctta tggcgattac attttgattg atgagaaagg aaagaaacgc tgatgaaaac
      721 acaacacaaa ttattgaatg taaaagaagt catttctgaa aaagtagcag aagctttact
      781 tattccgcgg tactggacga tagaaacccc tttagtagag gacgcaccga ctatgctatt
      841 tgaagaccta gtgaagattg caggatatga actgcattat caagaaaact tggaaactta
      901 tgaaaacgaa tattggttaa ccaaattaga gaactaaaaa ggagaaaaaa tatggctcag
      961 aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa aatggaggat
     1021 atgacgttcc tataccatga aacacgtgta cccaaaaaac attacaagga aaaattgtca
     1081 gtcgcagtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc gacgtactat
     1141 cgaacattgg aagaactgaa aaaacaaaat ggaaaatggt ttttccaagc cttactatgc
     1201 ttggaagcag gcgtcaaacc aagcacaatc aaaccttctg aataccaagc tttagaacta
     1261 acgtatggaa aatttatcga aacgaaaaaa gccaagactg tttcaagtga atggttggac
     1321 tactttgaaa atgtcaacaa gtatggcgca tgttacatca tgaaaaagga ggacaacgaa
     1381 aatgagtaat gtggtttatc aactaacagt ggaagatgtt tctcactttt taacaaacaa
     1441 agaacatttg aatgaagtag tcagacggat tcaaaaagac tttagttgta tggaagaaat
     1501 gaagtacttt attcaaagag tggaagaaca gttgtatatg gaaagcgaaa cgagtcaaac
     1561 gaaggtcaca gtcagccata atcatttaga cgtgtataaa cgtcgtcctg aaacggtaaa
     1621 aaaaggaaca gagcttctac gactagaaac agaaaaatac atcatgaatg tgtacctaga
     1681 aaggagtgag ctggtcgaaa actttgtcag ctacacgatt tgtaggaaac actattactc
     1741 agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag aaatggaaga
     1801 gctaaacagc aaaaatgcga cggtagacga ttattacctt gtatttacta gatttctgac
     1861 gtgtgggacg gaatctatcg tgttaatggg aatggattat gaactcgctg tacccgaagc
     1921 atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt acacgacttt
     1981 aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa atgagcttga
     2041 ctgtaaacga atgccatatc aacgatttga aaggaaaaaa ggtcaataac catttatacc
     2101 gaaaagtgag aatgtgtgaa ataagaacaa aaaaccttga aattaaggga atggttctta
     2161 tttcgcacca atcgctccaa ccgcccccgc cgctcaggtg gtgcgaaata agcgggcaac
     2221 gaccgataac ctaagagaaa gaaggaaatc catatgaata aaatcattcc tctgttgttc
     2281 gtactgtttg ttggcgagat acttatgcgt catgctgcag ctgtacattt aaacttcgta
     2341 gtattcttag gaatactggt attttgtttg atcttttggc gatttgtatt agcccttgtg
     2401 agtctactat tcaagatgat tgcagccata tttttcatag ggttatttcg atgaaaggag
     2461 agagtactaa tgccaattgc aataggaaat aaacgtttac ccgtgacgtt ggatgaaaag
     2521 agacaaaaag aattgcagga actcaaacag aagtacggca aaagtgaatc caggattatg
     2581 tgtattgcgt tagatttatt gattgcacaa gaaaaagcag ggtttgatgt accagcactc
     2641 aaaaagtgac gtcacttttc atttgaagaa acaaaagtga tagcacttta cgcacgtgtc
     2701 gatacgtgtt atgattattt tgtaaggagg tatagtgcac aatgccactt acgggaaaag
     2761 aaatgcttaa gttattgaaa aaaaatggct gggttgagcg aaggcaagag ggatctcatc
     2821 atcatcttta taaggatggt gttagaatca ctgttcctgt tcatgccaat caagatcttg
     2881 gccgaggact tgagcaaaaa attttaaagg atgcgggatt gaaataatct cgcattcgat
     2941 taaagaaagg attgactgaa atgttaagtg agcctttagc aaaaacctat cctgctattt
     3001 ttagcccaga agaaggtggt gggtacttta ttgaatttcc tgatgttcaa ggtgcttaca
     3061 caggaattaa cgaagacgat atatcttatg gaattgctat ggcacaagag gtgttaggta
     3121 tggttttagc agattatatt gagcatgaag atctacttcc agaaccaaca ccaatcaata
     3181 agatttctgt tgaagatgat tcttttacta ctttaattcg tgtagatgtt gccaaatatt
     3241 tgaaagacac agaacttgtc aaaaaaacct taacaattcc tcaatgggcg gataagctag
     3301 gcaaaagggc tggcataaat ttttcagtcc ttctaacaga atcaattgca gataaagcag
     3361 ataacattct tcgttctgga agaaataatt agaactcaaa agagtcagaa acttaaaaat
     3421 ctttggcttc tttttttact acaaagatgg cttctttaaa ttatttgcct gattgattta
     3481 aaaaaattgc gcaaagacta cgagctaatt aagttgctcg tggtctttct ttatgaaaac
     3541 tttttagatc tagataaaaa cattgagaca ctttgaaaaa tttttttaat agtaatgggc
     3601 gcttctatgt caagctgatt cttctttcgg taaattaatt ttacttagaa tatcactatc
     3661 ttgataaaaa atggtgtatt cacaaattct ggcataaaaa atacctactt tctagttaag
     3721 gttcctgaat aagtcgcaat ccaattattt ccagctgatt tataactttt taaatataat
     3781 tttccataat accaataacc attacttttt tctgtccaat ttcgttattg agaagatcta
     3841 gaaggccagc caactttatc aaaaatcatc attcctgtca ctcgtatcgt tttagaagct
     3901 gctcgagtaa gtaatggaga aactgttaaa taattttaag taacagttaa gtcgttagtt
     3961 gggttatcaa tggctattcc gtccttatct gaacctcgaa acccaatcat agaaaaagtt
     4021 ttttgaacaa gttttaacag ataaatcaag ttatatacta gaaaattcgt aaaatatatt
     4081 tttaatgtca taatcgtttg atttttctgg attgacattt atttataaaa aatagtgcca
     4141 ttaaaaaaga aaagtgacac accaataaaa gtcatatcct gtgacattat atggcattat
     4201 ccaaatagaa taaccggttg acatgtaagc ggattccttt tataatttta ggagtataag
     4261 tccttttcta aaaatattaa ccggttatca tgaaagggga tacaaatata tcgttggagg
     4321 ataaaaatat gtcaaaggtt aacgtattta aaaatcaatc ctatttacaa agttcagcta
     4381 cattattact attttttgct tcttggggtg tttggtggtc attttttcaa ctttggctaa
     4441 catctgaatc aaatggttta gggttatctg gcagtgctgt aggaacagta ttctcggcaa
     4501 attcgttagt taccttaatt ttgatgttta tttatggaac attacaagat aaattgtata
     4561 ttaaacgaaa tttattaatt tttgcttctg tattagcgac acttgttgga ccatttttta
     4621 tatggatata cgggccattg ctagataaca attttaattt aggcattatt atgggagcac
     4681 tatttttgtc agctggatat ttagcttctg taggagtttt tgaagctgtg tcagaaaggt
     4741 ttagtcgttt atttggcttt gaatatggac aagcaagggc gtggggatca tttggttatg
     4801 ccttggtagc gcttttggca ggatttttat ttgtaaaaaa tcctcattta aacttttggg
     4861 cgggatcttt ctttggttct ttactattgt taaatttatt attttggaac cctaaagttg
     4921 aacgggaagc aaatcaaaat tttaatcaag aacaagctga atcaaatagt attccttctt
     4981 taaaagaaat gtttgatcta atgaaactgc ctcaattatg gacgataatc atctttattg
     5041 tttttacatg gacattttat acggtattcg atcaacaaat gtttccggga ttttatactg
     5101 gtttgttttc aacatcagct aatggtgaaa aaatatatgg gacattgaat gctattcaag
     5161 tattttgtga agcgttaatg atgggaattg ttccaatcat tatgagaaaa ttaggggttc
     5221 gaaatacttt gttattaggt gtaaccatta tgtgtgtacg aattggattg tgcgggtttg
     5281 cctcgacacc attatctgtt tcatgcataa aaatgttgca tgctttagaa gtaccattat
     5341 ttacattacc aatgtttcgc tattttacac ttcattttga tacaaagcta tcagcaaccc
     5401 tctatatgat aggatttcag atagctgctc aaattgggca agtgatttta tcaacaccat
     5461 tgggaatatt aagagacaac gttggctatc aaccaacatt taaaattatt tctcttattg
     5521 tattactagc aggcatatat gcattcttta ttcttaaaca agatgataga gatgttcaag
     5581 gggatccatt tattcgagga taatggaaac caacagaata aaaaacaatt ttatctagaa
     5641 agtgagcgaa tatattatga caaaaagagc tatagaatta acaaatacac gttatcgttt
     5701 gggatatcat gtgtctgcac catcaggatg gattaatgat ccaaatggat tttgctattt
     5761 tgatgggtat tatcatgtgt tttatcaaca tcatccatat agtgctgaat ggggaccaat
     5821 gcactgggca catgctagaa gtaaggattt agttcattgg gaatctttac cacttgcttt
     5881 gactcctgga gatcaagaag atgaaggtgg ttgtttttca ggaagcgcta ttgaaaaaaa
     5941 tggtgtgttg tatctgttct atacaggaca tcactattat ggagacaatg atccagatca
     6001 tttttggcaa aatcaaaaca tggcgtatag tacggatggc attcacttta ctaaatatga
     6061 aaaaaatcct gtaatagcaa aagcaccaga agataacaca catcatttca gagatccaaa
     6121 agtatggaaa cacgatgaaa aatattacat ggttttgggt agtcaagaaa aagatggatt
     6181 aggtcgtgct attgtttatc agtcgttaga tttattaaat tgggattaca ttggaccaat
     6241 ttctaaagcg aaggaagtac ttacagaagg atttatgtgg gaatgcccag acttttttga
     6301 attaaatgga aaatctattc ttttattatc ccctcaggga attgaagcaa atggaaaaga
     6361 ttatttaaat ctatatgaaa caggctactt tatcggtgat tatgattatg agacagcaaa
     6421 attcattaga ggaaattttt atgaattaga taagggacac gatttctatg caacacaaac
     6481 aactttatct ccagatggta gacgtattgt tatggcttgg atggatatgt gggaaagccc
     6541 tatgcctgaa aaagtagacg gatgggcagg agcattaacg cttcctcgag aattagagtt
     6601 aattggagat catttgtata tgaaacctgt gagagaatta gaacaacttc gagttggcag
     6661 cggcgttgaa acaaaagtta atttggcagg agaaataata atcgctcaag atgcttcatt
     6721 caaagaagct ctaattgatg tactattgac taaatcaaaa aatcaagaaa tcaaattttc
     6781 attaaaaaca aaagatgaag agttaattaa tattttttat tctaaggata aaaatgaatg
     6841 taccttaact cgtaacggca aaaatgatcc aaggtatgga accattcaga tgtgtgatag
     6901 attatcactt cgagtgttta tcgataaaag ttcggcagag atttttatta atgatggcga
     6961 gcttgttttt acagagcgtt attatacaga aaatcaacca agtatgcatt tgagtgttag
     7021 tgaagaagct tttgtctcga gtacaattta tcatctaaaa aaagatgcaa tttcttacga
     7081 gtaaggaggt tttatgatgt taattggtag catagaagca ggtgggacta aatttgtatg
     7141 tgccgtggga gacgaggatt accgagtaca agaccaaata atttgtccaa cgaccacccc
     7201 cagggaaaca ttgaaaaaaa ctgttgacta ttttaaacag tttgatataa atgcattagg
     7261 tattgcatca tttggtccaa tagaaattcg aaaaaattca ccaaaatcag gatatattac
     7321 gtctacacca aaggtgggat ggcgaaatgt taatgtgtta ggttatttac aagatgagct
     7381 gaatgttcca atgtcttgga caacggatgt gaacggatca gcttatggag agtacatcat
     7441 gtccacttta tcgaatgaaa aaataaattc attagtttat tacaccgttg gtactggcgt
     7501 tggtggtgga gctgttgttg acggaaaact attaggatcg tttggtcatt cagaaatggg
     7561 ccatacgttt gtaaaacgcc atcctgatga tattgatttt aaaggaattt gtccttatca
     7621 tggtgattgt ttagaaggct tagttgcggg accaacgttt gaagcacgtt ttggaaaaaa
     7681 gggtgaagac gtttctttaa cggatcctat ttgggatgta atggcctatt atctagcaca
     7741 agctgtgatt caaacaacat tgattatacg accggataaa atagtatttg gtggaagcgt
     7801 agtaaatgaa aaattgttag aaaaaattcg tgttaatgtt aatgaattat tggatgatta
     7861 cgtggaaata ccaccgttaa gtaaatatat tacgcgtcct ttaattaaaa ataatggatc
     7921 ggcaacacta ggaaactttg ctttgggctt gaatcaatta caagaataaa acatagaaaa
     7981 aatatcgatt atcaggtata gtagttctat agagttcttt ttttcgatag ttaattattg
     8041 cattatttag gagagacaag agaatgcgcc caaaattaga agatgttgcc aaaagagcaa
     8101 atgtatcaaa aacaactgtt tcacgtgtat taaacaatcg tggctattta agtcaaaaaa
     8161 cgattgataa tgtttataag gctatagaag aactcaatta tcaaccgaac gttgtagcgc
     8221 gtcaattatt tcaaaagaaa actaacattg tcgggttact atttcctaca gtagccaatc
     8281 ctttttttag tgaactcgtt gaggcattgg aaaaaaagtt atatgaaatt ggttataagg
     8341 tattaatagg caattcgatg aataataaag aaaaggaaac aaattattta aatcagttat
     8401 tgtctgatca agtagatggt ttaattgttg gtacacataa ccaagggatt caggaatata
     8461 aatatcagaa tttaccgatt gttgcaattg atcgagtgat gaatgaagat attccagttg
     8521 ttgaatccga taactacaat ggtggaaaac ttgcaaccaa gttattaatt gcacaaggtg
     8581 caaaaaatat tattcataca aacggtccaa ttgacttaca gactccggct aatcgtcgga
     8641 gactagccta tgaagatact atgaaagcat atcagttaat tccacgaact gtgacattag
     8701 atttcaatat ttcttatgta aagaaaaaac agattttttt ccaaatgttt gaggattatc
     8761 ctaaaataga tggtattttt gcttcaaatg acatcgatgc ggccctaata ttacaagtag
     8821 caaaagaaaa ggggttaaat gtaccagctg atttattagt agtcggatat gatggtactt
     8881 taatgactcg gagtatatta ccagatctga caacagtaat acaaccaatt aatgatatag
     8941 cagacacagc tgttgctatt ttgatgaaac gaataaataa agaagaaaca aaaaaggaat
     9001 atattttacc tgtcacatta tggatgggga agacatcagt aaagaaaaag gacaattaaa
     9061 tttataaaga ctatcaatga taatttcatt attaattcgt gatataaaaa gttgttaaat
     9121 ctcttataat agactatgat acaaaatcat gcttttaatc tgtgtagatg ttgccaaata
     9181 tttgaaagac atagaacttg tcagaaaaac cttaacaatt cctcaatggg cggataagct
     9241 aggcaaaagg gctggcataa acttttcagt ccttctaaca gaatcaattg cagataaagc
     9301 agataacatt cttctttctg gaagaaataa ttagaactca aaagggtcag aaacttaaaa
     9361 gtttttgatc ctttattttc aagtctgatt tgacagattt tcggcagctc atttatttat
     9421 ggtcagaaat agttgctgaa attttaatct attagcaaat agtccagaac gtatgaagag
     9481 gattcggata tcatttgtca ttaacaccat aaatatttat aaaattcgtg ttcacctaca
     9541 attaaattta atatataata ttcgttgtaa gggcttataa ttttttgtta atgaacaaga
     9601 tatgtaattg atagtgtcaa tagctattca aaaatgtagg cttttggccc ataaataaca
     9661 aggttactta tatgataact ttttttcttt ataatcttta atccgataga gctttccagt
     9721 agtttgaata atcttagcgt ggtgcactaa tcgattgatt agtgcatttg tgttcagtcc
     9781 attgagacat aatagtggat agttttactg agaaaattga taaaaaatag agatataatt
     9841 caaatacgta tataatgaaa ctaaagataa atacggagga aaatatatga ttataaaaaa
     9901 cttaggaaat gttaaatcaa tagatataca cgttaaagat ttgctgattt ttactgggaa
     9961 aaataatacg ggaaaaaca
//
LOCUS       DAGCYL010000099         9938 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.100, whole genome shotgun sequence.
ACCESSION   DAGCYL010000099 DAGCYL010000000
VERSION     DAGCYL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.100"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            246..452
                     /locus_tag="MB216_001857"
     CDS             246..452
                     /locus_tag="MB216_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBT4526661.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(654..1655)
                     /locus_tag="MB216_001858"
     CDS             complement(654..1655)
                     /locus_tag="MB216_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4526662.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1660..3573)
                     /locus_tag="MB216_001859"
     CDS             complement(1660..3573)
                     /locus_tag="MB216_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBT4526663.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3741..4247)
                     /locus_tag="MB216_001860"
     CDS             complement(3741..4247)
                     /locus_tag="MB216_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4526664.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4407..4847
                     /locus_tag="MB216_001861"
     CDS             4407..4847
                     /locus_tag="MB216_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBT4526665.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4873..6030)
                     /locus_tag="MB216_001862"
     CDS             complement(4873..6030)
                     /locus_tag="MB216_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4526666.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6033..6389)
                     /locus_tag="MB216_001863"
     CDS             complement(6033..6389)
                     /locus_tag="MB216_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526667.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6687..7661)
                     /gene="sgrA"
                     /locus_tag="MB216_001864"
     CDS             complement(6687..7661)
                     /gene="sgrA"
                     /locus_tag="MB216_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBT4526668.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7857..8696)
                     /locus_tag="MB216_001865"
     CDS             complement(7857..8696)
                     /locus_tag="MB216_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBT4526669.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8881..9768
                     /locus_tag="MB216_001866"
     CDS             8881..9768
                     /locus_tag="MB216_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBT4526670.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         2992 a         1988 c         1497 g         3461 t
ORIGIN      
        1 tggggaaatt cttttggggg tatcctaaat cttttctatt atattcccat attttctatt
       61 attggtctag acgtatgatg gtaaactttg tacctatctg attgctgaat cattaattgc
      121 ttatcattag ccatctttgt ttcaagttcg ctagatgatc attttattca catcctaaca
      181 aacttgtcac atttatagat tcttaatcta tttatcatac aattataaaa aaggaggtgc
      241 tgcttatgaa agctatccat atttctttgc tttttttcgc tagtgcattg attttatttg
      301 ctattcatct ttgttcccag ccttatatca ataacaacgg aatattagtg gaaccagcat
      361 tcttttgctt accactcggc tttttgtccc ttcttgcctc cgcaggcttc ggatttgttg
      421 ctttttttaa acgatcaaaa caacaaatct gacttataaa atttacgtaa aagagatcgt
      481 gacaataatc gtgtgatatt cccaaatttc gaataaaagg tgccccttgc catctctaac
      541 tcctcagtca caagttacaa tattcataaa ccatgaaaag cttgtttatt gaaattgctc
      601 cttgtgactt ggagctaagt gacagattac aacctctttt gcatcataag actttactcc
      661 ttgaaaaaca ttactaactt ttgatatgtt tcttgcgctt gtttattgct gaaatcaaac
      721 tgatattcat ggttcaaaac agcatttttc tccgcataat ctacttttgt gactttcacc
      781 cccgctttct caaatacaga agttacttct tccatttgtt tcgtaaacgt atttgtattt
      841 ccatccgtta agaatgtgtt ttgcgtccaa cgatccgcat gtctgaccaa ggctaaactt
      901 ttgaccttct ctgattgttc aaagtcctta ttatcaaagt aagcccatcc acatttttca
      961 taaagaaagc tagagatttt atcacttact tgagtaagtg cagaaggatc caacagacca
     1021 gacaaagaaa taaacttcgt aatctgcttg ttctccaaag tcggatcaag atgatcagct
     1081 tttttaattt cttcattttg aagtgctaac aaatattctc cactaatttg tgcacctgcc
     1141 gaatttccac caatcgaaat attcgaccaa tctgcttgat cttttatctc ttttttagct
     1201 gcttgaatgg cttcgttcaa ttggataact ggtgtaggat gttttgcttc cggtgatcgt
     1261 tcataatcta tatttacgat gatttgctgc gtgtctgaac atagatattc caaataatca
     1321 cgacaatcat ttttatcccc tcctacatat gcgcctccat gcatccagaa aagaatcggt
     1381 aattttttct tttccacttt cggtgtgtag atatccatat tattattttt gtactttgaa
     1441 ggataggata tttctttttt tatatcgatt tgttcgattt tttgctggaa atcatctggt
     1501 cgggcatata cttgttcttt ttcacttgtg tcaaattgtt ttctaacaag caagctaatc
     1561 ggctttggtg tgatttgatt aatcaaaaat acagtgacta aaataactac gatgataact
     1621 ggtactgcaa tcactattcg cttgaaccat ttcattcatt taccctctct ttcttcgcca
     1681 aatccatgtg ataatcgtgc taacagataa aaatccacct gaataaatga gaagcgaatg
     1741 tttcttttct ccagttttcg gcaacattct tttttttgca tctgtctttt tttgtacttt
     1801 cactgtcatc tgaatcggtt gcgtatcatc acttgattga tcctctcgag gttcattctt
     1861 tcccttattg tctgcattct ctgtcggatc ttgctttttt ggtggagcta ctgggatttt
     1921 tttgtaatag aaaataatag ttggtatcgt ctcattaaaa atcccttcag tcggtccttc
     1981 aacatttgct aattcgtatc cttcgaatgt cttttgctct gtcatataat tttctccata
     2041 agctccgtaa agctggattt cgtcctggtc tttgagtaga cttttattgt tttcatccac
     2101 atactttacg tttatctgat catggaacac tactttcaaa aagtccgtta catgtttcgt
     2161 gtaggcagca tggtcaactt cctgtgcctt tccatgccct gctccttcta ctaataaaag
     2221 atctttatac cctcctgata actcatataa tttttgcgcg ttagacacag gtactacatc
     2281 atctgctgtt ccatggataa tcaatttagg caatgaatct gacattccaa tcgtaggtag
     2341 agcagctttt gtatccaaat gcatctcctg atcaaaatag tattgtttca aaaggcccat
     2401 aaataaatct tcatgctctt gatccccaat gatgtctaac tgatttttta tggcatccgg
     2461 tagtgcgggg gctactgtct ggctgtacgt aaaacgtagt tgttcatcaa ttgatgcaaa
     2521 tccgcaattt tcaataattc ctttgacatt gacaggaata tctttcgcta atacagacat
     2581 catcgtagct gctcccatcg aaccaccata aagtaaaatc ttttgagatg gccgctctcg
     2641 aacttcttga tttatccagt aaaggacgtc atctgactca tattgtccat aggtcacata
     2701 atcgcctccg ctattaccag tcgctctgga atcaacaaat aataaattat acccggcctt
     2761 ataaaaatca ttgtattctt ctgtaacgat cccgttattc caattccctc gaaatccgcc
     2821 atggatcatg actgttttat ctgaaccttg atcgacataa tatgcatata catccatcat
     2881 tttgccatct tgtttgttta gtacagtttg atgaagttgt tttgtatttg gatagaagcc
     2941 tgtgttttct gtaggcgttg tcccatcatc tgtacctcca agcatcgaca tcaacacact
     3001 gttttttgca aaagcgatat cgtacaatag ataagtagca atcagatgtt tgataatctc
     3061 tttatctaca ccataatcat taacagcaag ctcagtgact aataactcta ttaattttga
     3121 cttgatgtct tctgttggtc cattaagtat ttctgtcatc tgtttaccga agtaattcaa
     3181 atattcttta ccattgaaat tttcaccttt accgatatca tcattttctg tcccattctc
     3241 tttgtcactt actttagcag aagaggattc gtcgtttgac atttttgtag ctgttgcttc
     3301 ttctgtttgt tggttatctg tggtagcggt cgtttcttga tctgtgctag taacagatgt
     3361 ctcaaccgtt gtttcagtat ttgtttctgt ttgtgaagta ctcgatttcg tttcatttcc
     3421 agcatcagta gtttctgtgg tttctacctc ccgagattcc gattcggaag aaatgctctc
     3481 tccatatact gtccattgcg ggacgcttgt acttacagca aataagatga ccaacgctcc
     3541 ctttaatact tgttttgttc tcaagctatt cactcctttt tatgtttcta ggagaattat
     3601 atgtaaacgc aaacattttt tatacaaaaa ataaatggtt tatcccataa aaatagaaca
     3661 ggattatttt ctcattttac gtaataaagc agaatggata aaccagtatc cattctgctt
     3721 tttttaaacg aagaaacaac ttattgctcc aaaaatgtca gaaatcgttt atcccaaatt
     3781 ttctcgtaaa aagcgattgt ttcctttgct ttcataaaag gattatcata agttgtcgtt
     3841 gctcctctac acatcataag ctggtatcct aatccatgag caaacttcac agtcgtatct
     3901 atacaatact ctgcttgtgc cccacataat tcgatcgaat gacagtccaa ttcagttaat
     3961 aaaacactca aattagtctt ataaaatgaa ttcgcatggg tttttctcac aaaataactg
     4021 tgctcaggaa tagataattc cgacaatagc tcccatggtt ttgtttgtgg cactaattga
     4081 gcatcttcgt gttgaacgaa aataactggt ttcccaatcg tttcgtatgt tttgattctt
     4141 ttattcacta atgctgtcac tgtttctaat tcgtgtatcg gcgaagaatc aaaacatacc
     4201 cctttttgta aatcgatcac tactattgcg tcggctgtgt ttgacatact tgtgccttct
     4261 ttcttttttc ttgatttaat agtatttctt atctagctag ttccattata cgtgacaaca
     4321 ttttcttatt ttacacctct aaatggcact tttcgactat ttcttcatgt tattatttca
     4381 taaatactta gctattggag gaatgtatga tgattaaatt gtatacaggt tctggctgct
     4441 cgtcttcaag acgcgcaaaa cgttggctta tcgtccatga tataccgttt atcgaacaga
     4501 acttaattcg tcaaccacta accaaaaatg aatttatcca cttactaaaa ttgacaaaca
     4561 acggcgtgac cgaaatgctc tctactcatt ctattagtta taaaaatctt gttcacagaa
     4621 tagatgaact cagtttgagt gaactatata ttgagattcg tacatcccct tctttattga
     4681 aaaaaccgat tatctacgac caatctgcaa aacttttgac tggatttagc gaagataaaa
     4741 tcaggatgtt cctttctaaa actaaaagaa agcaagagct caacgaactc ttgctttctg
     4801 gtcatcttgt cgattttaaa gaaaataatc tttttgcaat gtcttaattt tggcataaac
     4861 aatctgcttt tcttaattaa cgaaatccaa tttttctaat atggatctat ttttctcatt
     4921 cctgattttt atgatttcag ctttcagacg cgcccaatca ttttttgtag gttgtccatt
     4981 ccagtaaaat atctgtgtat tcgtttgatc atatgccttt ttgtcgatag gataatcgct
     5041 aatgacaata tcaggtaact gatcagcgga tgcactatat tttattgggc gttcagtcat
     5101 agataatatc cttttctttg cccataattc ctgtgtttta ccatactttg attgaatata
     5161 gaatatgaca ggttgttcgt gttttataaa aatatccctg actaacatac agtattgaaa
     5221 aatcaaccgt ctgttctttt cataataagt attaagctct ggaattctgc gagctacccc
     5281 actaaaaaac tgttgcatga ttggatacat cactggaaat atatgctgaa aatctctgtc
     5341 ttctgctgat tttccaaaag catccacctt cttttttgtc ccaaaaaccc ttgaatacaa
     5401 atggatcatt gccaaattga catatagata atttttttct gaagttgtaa aacaaatctt
     5461 gaagtttttt tcaaaatttt ctacaataga cgattcaatc cagccccaac tttcttgtac
     5521 tttatctgga gtatccaaca aatcttcttc caaataggtg ttcattacac caaacataaa
     5581 ataaaggtaa gcttgctctg ttggagtgat gtgattcatc gaccagaagt ttgcttcaaa
     5641 aaaaggctct agtatgtaat gcgtaaactc ttcttttgtt atcaatggat gcacaaattt
     5701 gttcatctct tctgggacat acgtaatcga gtggccttgt ttcattcgtt gcaacgaaat
     5761 tcttagtagg ctgatccatt tctttctatc tatactacgc gtatatggcg catgtttcgc
     5821 aaatttttct aatagttgac gtttattttt tatacttgat aatatatcag aatgattttc
     5881 gtcgaatgac tgccaataca tcaagtgaaa aaagtatcgg atacggtatt cttctccttc
     5941 tatcggatta ccttttttca agctgcaaga taaatgatag tttttcaaaa tagtattaag
     6001 ttctcttaat tttctttgaa tcgtacgcaa acttatataa ttttttgact aagattttct
     6061 aaggattgat aagtaccttt gtaaagtgaa tccagtaaaa tataaccaat cgttccctct
     6121 aaaagtatag aataaacttt actcagtaaa aactgagaag aaaactcgac ttttatcatc
     6181 ttgttttctg aaagaaaaga taatttatat tctggatacc tttcgcaaaa cgattctgct
     6241 tgttgaatca ccgaattcat cttagccagg gtgtagttta gatctaaagc gatatccttc
     6301 accacatata atttctcgtc tctttcataa caatagttca aaaattgata tacttgcctt
     6361 tcattgatat caataaattc ttcaaacaaa gtacatactc ctttttatct attatttaga
     6421 cttattccga cagctacatg aataacgtac aaatagattc gttactgatt tttcagagat
     6481 actaccatgc gcagtgtgca ttgtcaatta ttcttgctct tattttccat ttgtgttcct
     6541 gttatttcct gtttctcaaa tatgttagta aacaaaaata gatctggcaa agctacaact
     6601 tcgccaggca atacagcact ctcgaatgaa atattcactc aaaaatctat cgttttattc
     6661 aattttagat ctgttgcttt tattctttat ttcactcgat ggaagagaga acgaaaatag
     6721 cttgctccta caatcaaaat gagtaacaca cccatccatg taattagctt gttcaaggtt
     6781 ctactaccag tttctggtaa atcgcttttt ctattatctg tttctttcgc tttttcttga
     6841 tgatcaattc cacgagaaac aaaatcaggc tgttttagca cactttttag gttgctccct
     6901 gcatttctct tttgttcctt agttggtatg ataggtacca aatcaagttc attatttact
     6961 tttcctgtgc tgctttcgct gctcgtgctt cctgtgctac tctcactact catgcttcct
     7021 gtgctacttt cactactcgt actctccgta ctgctttcgc tgctcgtgct tcctgtgcta
     7081 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgttttctgt gctactttca
     7141 ctgctcgtac tctctgtact gctttcaccg ctcgtgtttt ctgtactgct ttcactactt
     7201 gtgctatccg tactgcttgg cggtccaaca actgtccaac tgcttggttt caaattctcc
     7261 gtgatcgtca tctgtccatc ataaaaaaca atcaaatcaa ctttatcttt actcttgatc
     7321 tcatcaatcg atagttctgc agaaatcatt attgtatact caataccagc ggtccattgt
     7381 acgtctttta aataaacttc cccaataact ttttctgaac ttccattacc atcccaacca
     7441 tatgttagaa cagcatgtac atcactagca ttcacgcttt tgtttggttt gactggaata
     7501 gtaatgactt gttgtgtcat gtcataaaca atccgatcag ttttcacagt aaatgaaaga
     7561 ttctcatttg cccgttcatt tgcagaagta ctgattccaa tcattgctgc aattatactc
     7621 agcccaagaa taacaaataa tacggtcgct gttttcttca tttctatcac tctccatata
     7681 attaccttct atttgataaa ggaattatat aaaatgaaaa tgatgagaac acgacagtca
     7741 gttgtgaaaa tctaacaaaa gatgtcgtgt attcgcttga atttgtttac tttttcgttt
     7801 aatcaatgac agacttaaaa aagcaagacc gatatcagcc ttgctttctt catcttttat
     7861 tgataaacag cagtgtattt cttcatccgc tttgttttat atgcatagag taacaaacca
     7921 acaatcaaga ctaacaagaa taatgtagtt gatatagatg cttcagcccc aaagtttccc
     7981 ccagtaatta ttgtattttg cgattggatc agagtggaga aaaaaccttg ttcttgcgtt
     8041 ccgctgactg gtatacccaa aatagctcct tgtgtccaat tccaagtaat atgagcacca
     8101 atagccgtaa ataaacttcc tgtactgtag ataagtgcgc tgaaaaagca acctgctaaa
     8161 aagatattga aaactgccag aaatgtcatg ttaggattca atccgtgcaa aagagcaaat
     8221 aatgcagcag atccaatcat tgctattaac ggaccaaatc taagcatctg atgctgaaaa
     8281 aagccacgga ataaaagctc ttctgatgcc cccactgcaa taaataaaat aaaactcgtt
     8341 atcagcggca caatgtttgc gtttcctgaa tctaaccgat acgcccccgt caaatataat
     8401 ggactagtaa taagcagaaa cactccaatt ccaacaacga gacctcccgc taaatacccc
     8461 cagtcttttc ctcgaacaga aacaccataa tcttctattc tttttccttc gaccttacta
     8521 gcaagaaata aagctagtaa aaaaacagct aaatgtccga ccgtaccggc aattgctcca
     8581 atccattcac tatttccaat aaacatatcc gcaagtgcat tattgacaag aattcctaaa
     8641 acaaaaccaa ctaagactat taaaacagat ccaatcaacc gaataatcct gatcatcttt
     8701 catccctcct ttatattaag tttaacaacc cttttctttc ttgtctcgtg aaaactatgt
     8761 aaatgttctc catcatagag agaatagatt tttacgaact aagaatatct acagcaacct
     8821 tacgaaaatg aaagactgtc tccctaattc ttctgttaga ctgtttttaa taaggaaagg
     8881 atgggaaatt ttttgaaaaa acaactacta attatgtgcc tttcagcatg tgcaatttta
     8941 ctattctcag gttgtagcaa gtccaatgaa acgattgcaa cgtttaaagg tggaacactt
     9001 acagttcagg atttttatga taacccaaaa gttaaatcaa ataatcaaac actattaaaa
     9061 aaaatgattg tcttcaaagc atttgatgat gtatacggaa aagagatttc ttcaaaggaa
     9121 gtaacaaaag aatataatga acaaatcaaa aaactcggtc caaactacaa ggaacaatta
     9181 aaagcagttg gccaaacaga agagacatat aagaaactct ttaaacagat gctggctttc
     9241 caatacggtt tgaaagccaa tgtaaaatta acagataaag atctagacac tgcttggaaa
     9301 gaattctatc cagaagttag tacacagatc attctatttt cgactaaaga agaagcagat
     9361 aaagcaaaaa aagaagcaaa tgaaggagaa aatttctcca agcttgttca agcttatgga
     9421 aaaaataaaa cattaaagga aactgatgga aaaatgaatt ttgattcaac caatccagag
     9481 attcctactg aagtaaaaaa agcagcattt aaactaaaaa acggggaagt ttctgatatc
     9541 attcctgtaa cagatccaac gacttatcag caaagctatt atctagtaaa aatggtaaaa
     9601 aaacaggata aaggttctaa taaagacaaa tacaaatctg agctagaaaa aattgctacc
     9661 gaagcaaaaa ctcttgatac ggaattcatg gataaaacaa tccgtaaagt gatgaaaaaa
     9721 gacaatgtaa ctattaaaga tccttatgta aaaaatatat tcaaataaac aaatttgacc
     9781 caattgggga tgtggcaaaa tttgagaagt gatttttgga aattccttct tatttttgag
     9841 gcaaaccact tttgccacaa cttcatcggg gccttttttt atttgtacca aaacaaatca
     9901 ctggatttgt tacaatcatg aggatataat ttaaaaaa
//
LOCUS       DAGCYL010000100         9924 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.101, whole genome shotgun sequence.
ACCESSION   DAGCYL010000100 DAGCYL010000000
VERSION     DAGCYL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.101"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            1..357
                     /locus_tag="MB216_001867"
     CDS             1..357
                     /locus_tag="MB216_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HBT4526671.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            373..723
                     /locus_tag="MB216_001868"
     CDS             373..723
                     /locus_tag="MB216_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HBT4526672.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            752..1531
                     /locus_tag="MB216_001869"
     CDS             752..1531
                     /locus_tag="MB216_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HBT4526673.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1542..2219
                     /locus_tag="MB216_001870"
     CDS             1542..2219
                     /locus_tag="MB216_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HBT4526674.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2238..3344
                     /locus_tag="MB216_001871"
     CDS             2238..3344
                     /locus_tag="MB216_001871"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBT4526675.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3334..4425
                     /locus_tag="MB216_001872"
     CDS             3334..4425
                     /locus_tag="MB216_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBT4526676.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4438..5178
                     /locus_tag="MB216_001873"
     CDS             4438..5178
                     /locus_tag="MB216_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBT4526677.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5466..7406
                     /locus_tag="MB216_001874"
     CDS             5466..7406
                     /locus_tag="MB216_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBT4526678.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7493..9415
                     /locus_tag="MB216_001875"
     CDS             7493..9415
                     /locus_tag="MB216_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBT4526679.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9695..>9924)
                     /locus_tag="MB216_001876"
     CDS             complement(9695..>9924)
                     /locus_tag="MB216_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBT4526680.1"
                     /translation="IDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAV
                     GSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3236 a         1647 c         2205 g         2836 t
ORIGIN      
        1 atgattaaag tagttattgc agaccgtatg ggtaaaggac aaaatgtggc caaaggggta
       61 gaagcagctg gtggaaaagc agttgtcgta ccaggaatgg gggcagatat gcgtttgggc
      121 gatgtgatgc aacaagagca tgcagatatg ggtatctctt tttgtggtag tggtggtgca
      181 ggtgcattaa cagcagcaac aaaatatggc tatccagaac gacatggtat gcgttcgatt
      241 gaagaaggga tcacagctat caaggatggc aaaactgttc taggtttcgg ctttatggat
      301 caagaggaat tagggaaacg tctgacagaa gaatttatca aaaagcatgg aaaatagaaa
      361 gagggacatg aaatgagcgt tcaaaagaca attcagcaaa aagttatcgt ctccggtaaa
      421 ggggaaacaa agcaacatgc ttttgcatca gcgttagcag aaattcaaaa caaaataatg
      481 gaacaatcaa acgtattatt gcgaatcgaa ccagtcaatg tggatatttt aaaggcggtc
      541 gaaaacaaat acacagaacg ctttctattt ttcttcttcc cacggcaacg tattagctat
      601 gaagtcgaac tctctgttac tgtggctgta acagaaataa aattggcaga catatcgttc
      661 agccagcaaa tgactgaaga tccaaatggg attgccattc catttgtaac taaaaaaatc
      721 taattttgaa tcataagggg gaaagaataa cgtggatttc gtaatcattc tagtcaaatc
      781 ggtcattatc gggggattac ttggttttgc agcatctgtc ggggcagctc gaatgtttca
      841 tgcacctaat acacaaggtt taggagcttt tagaacatta ggcgaaatga atgcctgcat
      901 gggtgatcca gcttctcatt tttcatttgg tttaggcttt ttctttaatg catgggcttc
      961 ttcagttggt gctggtgcat tcacacaaga tgtgacgcat cgaatcattc ccaattgggc
     1021 agcggcagca cttctagcaa aaaacaaaga tgtcacccag accatgcatg atccgaaaaa
     1081 aatggggatc attggagcgg ttatcggggc agtggtggta gccttcttaa acacaacagc
     1141 tgcagcaatt ccagaggcgt tacaagtgac ggcggtttcc gttttggtac cagcagcgac
     1201 gattctgatc aatacagtaa tgccagttat tttttggttg gcagctttag acgcagggaa
     1261 acgaactggt ttttggggca ctctttttgg tggtatcgcg caattgatta tgggaaatgc
     1321 ggtacctggt gttgttctag gaatcttgat tggtaaaggc gtcgatgaga gcggttggaa
     1381 tcgcgtcacg aagagcatga tgatcgctat tgttttatta tttattttca gtggattttt
     1441 ccgtggattt gatatggaaa tgatcaagag cttccagctt caagtgccta attggcttga
     1501 taacttgcac aacatcttta gtgtgaaata ggaggagaat catggagaca acagaagaac
     1561 aagcaaaagt attagaagca aagcggcaaa agagcttttg gtttgcagac tggtcttttc
     1621 cgctaatcgt aggaattatg tccgctgctg tatttgcagg aacacacatg tatgtgatgt
     1681 atggtgtggg tgcattcaat gaagtttcga tcgtggcgat gttaaaagcc gggctggatg
     1741 gcggatccta tggcgcagcg gcagcgtttg gtgctagttt cttatttgcc agaattttag
     1801 aaggctcttt agttgggatc ttggacttag gcggttcgat cctaactggg atcggaattg
     1861 gggtaccagc aattctctta agtatgaaaa tagcggcacc aatcgataat tttgcactct
     1921 ctttattgac tggtttggtt ctaggtttga ttgttggtgg catcattgtc ttgattcgta
     1981 aatttacgat caatcaatca aattccactt ttggtgctga cgtcatgatg ggggcaggaa
     2041 attcatctgg acgttttttg ggtccattaa ttatattaag cgcgtgtgca gcatctattc
     2101 caatcggcat tggcgctaca ttaggagcac taggattcta tgcttggaaa aaaccaattg
     2161 ctggaggtgc tattttagga gcaatgattt taggagcaat cttcccggca gtaaattaat
     2221 agaaagaggg tcattaaatg tttgatactt tgattaaaaa tggaaggtta gttgatggat
     2281 ctaaaataga ggtcgctata gaaaaaggca tgattaaagc agttgcagca gaaataaatc
     2341 agccggcgca agaagtagtt gacttggagg gaaagtatta tttatccgct gggtggatcg
     2401 atgatcatgt ccactgctat gaaaagatga atctttatta tgactatcct gatcagatag
     2461 gggtcgaaaa aggtgtgacg acggtcattg atgctggaac tacgggagca gaaaacatcc
     2521 gtgaatttta tcaattgacg aaatcggtca agacaaatgt gtatgcgcta atgaatatct
     2581 ctaaatgggg cattgtcaaa caagatgaac ttgctgatct aacaaagatt caagaagatc
     2641 tagtaagtac tgcgttagca gaactgccgg aatttattgt cgggattaaa gcgagaatga
     2701 gtaagacagt tgttggtgaa aatggcatta agccattaga attagctaaa aaaatccaga
     2761 cagaaaacaa tgatctgcca ttgatggttc atatcggttc tgcacctcct gagttagaag
     2821 aagtattgtc aagaatggaa agaggcgatg tattgaccca ttgttttaat ggaaaaccaa
     2881 acggtatttt agatccaaag acagatcaaa ttaaaaactt tgtctgggat gcttacaata
     2941 atggtgtcgt ctttgatatt ggacatggga cggatagctt taatttccat gtggcagaaa
     3001 ccgcactaaa agcaggaatg aaagctacct ctattagtac ggatatctac atccgcaatc
     3061 gtacaaatgg cccagttttt gatctagcaa ctactttaga aaaattgagg gtagtcggct
     3121 attcttggga agaaatcatt gaaaaagtta cagcagttcc tgcacggaat tttcatttga
     3181 agaataaggg ccagttagct gtcaattatg atgcagattt gacgatattt gagattgttg
     3241 aaaatgaaaa ggaattgaaa gattccaacg gatttacacg tatagctaaa gaacaaattt
     3301 taccgattaa aacaattatt ggaggcgtca tatatgacaa ttagctatgg aaagtttgat
     3361 ttaaaagaag tcattaatgc atcgggaaaa atgacgatcc taggggtttc taaggtatct
     3421 gaaagtgtcc tagcagcaca acgatttgga ggcgagcatt tttttgagat gagtgatctt
     3481 gccatcaaaa ccggcgctta tctttctcaa ttactgaaag tggaagatgc gcaaatcgtt
     3541 tcttctgctt cagcagggat tgctcaatct gttgctgctt taatcggaca gggcgacatg
     3601 taccatgtct atcatcccta tacagaaaaa atcgctagac gggaaatcat catacctaag
     3661 ggacataatg tagattatgg gacaccggtt gaagtaatgg ttgctcaagg tggaggaaaa
     3721 gtcatagaag ctggctatgc aaatatgtgt acaccggaac atgtttccat gatgattacg
     3781 gaaaaaactg ctgcgcttct atatattaaa agtcatcatg cagttcagaa aagtatgctg
     3841 acggtggcag aaatggtaga agttgccaaa gctcatcgtt tgccattgat cgttgatgca
     3901 gctgcggaag aagatttatt caaatatagc gaaatgggcg ttgacttagt gatttactct
     3961 ggggcaaaag caatcgaagg tccgagtgcc ggactggtca taggaaaaaa gaagtatatt
     4021 caatggattc ggctacagtc taaaggtatt ggccgagcaa tgaaaatcgg taaagataat
     4081 attttaggct ttacccaggc actagaagat tatttaaaaa atgggagcga atccggtgac
     4141 tccatgaagc aacgattagc gccttttgtt aacgcaatca atcaacttcc tgacctttca
     4201 gcgaaaatag ttcaagatgg tgccggcaga gatatttatc gagcaagtgt aacgattaat
     4261 ggcgataaaa gtgcgaaaga agtcattcag gaattaaaag cacgaaatcc agcagtctac
     4321 acccgagaat atcaagcaaa taacggaatc attgagttcg acattcgttc cgtcagccaa
     4381 aacgaaatgg aaaaaattat aaaaagattg agagaaatca tgtaggaggc ttttaaattg
     4441 tcagttatcc caaattatta taaaaatcgt gtatgtttaa atgtattagc tcattcagtt
     4501 gaaaatgcaa aagcatgcta tgaagcagcg gaagggcatg tcgtactagg tgtgctatcg
     4561 aaaaattatg ctagcgatga agcagcaatc gaagatatga aaaggtacca agacgcaacc
     4621 aataatgcgt tgtcagttgg attaggcgct ggagatccaa accaaagtca gatggtttcg
     4681 cgtttatctg gggttttaca accacaacat gtcaatcaag tatttactgg cgtggggact
     4741 tctcgtgctt tattgggcca aaatgataca gtcatcaatg gattggtttc accaacaggt
     4801 aagatcggct atgtcaatat tgctacaggt ccgttaagtt cacaagcgcc agcagctgaa
     4861 gtgcctattg aaactgccat tcaaatgctg caagacatgg gaggtacttc aatcaagtat
     4921 ttcccgatga aaggcttagc acatagggaa gaatttaaag cagttgcgga agcttgcgca
     4981 aaatatgaat tctatttaga gccaacagga ggaattgatc tttcaaactt tgaagaaatt
     5041 ctgaaaattg caatcgatgc aggtgtaaaa aaagtgattc ctcatgttta tagttcaatt
     5101 atagataaag aaacaggaga tacgcgtata ggagacgtaa agatactatt aagcatggta
     5161 aaaaaaatta ttcactaact cattagaaaa taagaagctt attccagaat gaaattaaat
     5221 ggaataagct ttttataatt aattacggaa attgttaatg tagataataa tttagatgta
     5281 cgtaagcaga agtacagaaa tgtaagattt tccaatatta ttttattcaa cataataaat
     5341 cgtatatcaa tttttttagc gaaatttcta tttattccaa gaatttcagt tattacggaa
     5401 tcattttgtg cttaataaaa agaaaccgtt tttatatact ataaatgtag aaaggcggta
     5461 ggaaaatgaa aaaaattatt gcaatcacat cctgtccagt tggtattgcc catacataca
     5521 tggcagcaga aaacttagag aaagcgggga aagcagttgg cgccgaggta aaagtcgaaa
     5581 cacatggttc catcggtatt gaaaatgaat tgactgcaag agatatcgaa gaagcagctg
     5641 gtgtaatcat cgcagcagat actaaaatag ataaatcacg attcggcggt aaacctttga
     5701 taaccgtagg tgtgcaagaa ggaatccacc atgcagatga gttggtgaga gatattttag
     5761 caggaaaagc gccagtctat aaaagtacgg aggcaattat ttcttccgaa aataaaagtg
     5821 aatcagaaaa tttaggtaaa aaaatctata agagtttgat gaatggcgtt tcgtacatgg
     5881 tacctttcgt agttacagga gggttattga tcgcgatttc tttaacatta ggaggaacag
     5941 ctacacctga agggatcaag ataccagaag gaacgatttg ggcaacgatg aatagtatcg
     6001 gaagtatcgc tatggggttg atggttccaa ttctatctgc atttattgcg caaagtattg
     6061 cagatcgtcc aggacttgtt cctggatttg ttggtgggat gttagcggca aacggagcat
     6121 tatatggtag tgatgcgaat gcgggattct taggcgggat catcactgga tttttagcag
     6181 gattcattgc tttaggaatc aagaaagtgc gagtaccgaa agctttacaa tcgatcatgc
     6241 caattattgt tatcccaatt ttaggatcat tggcagttgg ttttgtgttt atctatgtta
     6301 ttggagaacc agtagctctt ctatttagtt cattgactca ctttttagca gggatgcaag
     6361 gaggaagtga aattgttcta gctatgatct taggagcgat gattgctttt gatatggggg
     6421 ggccgttcaa taaagtagcc tttttgttcg gatctggctt gattgcagaa ggcaattatt
     6481 ccattatggg gccaattgct gtagctatct gtatccctcc attagcattg gggctggcat
     6541 cattgatttt aaaaaccaaa tttacgaaaa cagaacgaga agctggtaag gcgtccttag
     6601 caatgggact gtttgggatt acagaaggcg ctattccgtt tgcttcaaca gatccactac
     6661 gggtgattcc aagcattgta atcggggcaa tgacaggaag tgtgatcgcg atgctttcag
     6721 gagtgacaga tcatgtagct catggcggtc cgatcgtagc ggttttagga gcagtggatc
     6781 acgtagccat gttttttgta gcagtgattg ctggtattgc tgtaaccgtg ctaatgttaa
     6841 gaatccttaa accgacatta caacaaactg cggttgctgc agcaaatggt gtgacagtag
     6901 cagatctatc agagactgaa acaagtgatg tttatacgaa aactgaaact tttgattcta
     6961 tcacggatat tatgtcagat gaagcaatcg ttttaggtga aacagcaaca acaaaagacg
     7021 gagtaatcga agatttagtc caacgtttag aaaaaacagc ggcgatcaca gataaaacca
     7081 gatttaaaga agtcatctat gatagagaac atgaaagtac aacgggaatc ggtatgggga
     7141 ttgctattcc acatggaaaa tcagcttatg ttcttaaacc aacagtggca tttgccagaa
     7201 gtcaatctgg ggtagattgg cattctttag atggaaaacc tgcacatatg atctttatga
     7261 ttgctgtacc tgaaaacagc caaggtgata tgcacttgaa aatactgcaa cgtctatcac
     7321 gtaaattgat ggatgatgac ttccgtcaag cactgatgga agcgccagat aaaacaacag
     7381 ttcaccaatt attaagcgaa atgtaacata agatgaaaaa tcgaccgtga tgagacataa
     7441 tagatgtgtt atcatgatcg attttttatt tagaagggag tttctgcaca ttatgaatca
     7501 gcgtcagcaa tctttgatct atcaattaag tgagaacaat aattatataa caacaaaaaa
     7561 gctagcagag catttccatg tgtcagaaag aacgattcat agcgatttga ataccattga
     7621 aacttggttg agagacaaac atctgccttt aatgtttgaa agaaaaaaag gtacaggtat
     7681 cttgttgaat ggtctgcctc acgaaaggga acaactgaaa cgagcgctta atgaagagac
     7741 tcaaacagaa atggataaaa aacagcttcg tcagttattg attttccatc tgctagtagc
     7801 aaaagacgga tttagtcttg aagaactttc agaaaaacta tatgttggaa aacgtacgat
     7861 tcgtaaagaa ttgacagagt tgcaaagctt ttttgagtat caccatttac agctagtatc
     7921 caaaacaaaa ctaggaactt ttcttaaagg cgatgaacaa gaaaaaagac agttgcttgt
     7981 taagacatta cgaaatatgc agaaagaaga tccacaaagt ccgtcgttga aagaattctt
     8041 cgctaaagat acattaaaag ttatccaaca tacattacaa gatgtttttt atgaaaacca
     8101 tttagaacag ccgaatggac tggctagtgt ggagattcac atctacttta tgctcgagcg
     8161 aatgaaacag taccagaaag tgaaattaag taaagacgaa aacgaagtcg tggaacacac
     8221 ccaagcacaa aagttaagta gtcaaatatt agcaaaacta gccaccatct atccaattga
     8281 attttctcct gatgaaatca attatctggc tttgcgtatc gccaatcttt ttacaaattc
     8341 acaagcagcc acacgttttc aaaaggaatc tagcgcactt gctgatcatt tgatcgaaca
     8401 agtggaacag tttctaggct attcattaaa agaagatcag ctactaaaac aaaatttaag
     8461 gtctcattta tcttcaactt attttcgact ccattatggt ttgcagattt ctaatccatt
     8521 gacgaaaaat gtgtttagta cgtacacaca acttttctta gttttacagt taattttaga
     8581 agattatttt gaaaaggaaa atttttatgt tccacaagag gagacagctt atttaacgat
     8641 ccatttccaa gcagcgatcg agcgacacaa acatcagaaa agtagaagat atcaaacggt
     8701 tttagttagt gaatattcaa aagctatggc tacttttctc gaagcacggc tgaacagaga
     8761 acttccagaa ttaacgatta tggatctagt tgaatataag ccagaattaa agagacaaga
     8821 ttttccctct gttgatttta tcttggctac gttaccattt aaacatataa gcattccagt
     8881 aatcgagatc tcaccaatga tcacagaaac tgatctagcc tatttgacaa aatatatgct
     8941 ggagcatgtg cctatcaaga agaaaaaaac atttgatcta gcaagcttta ctcatccatt
     9001 tctgatcttt cctcaacttg aacggacaga cccagccgat attttaaact ttatgggaaa
     9061 cgtcttagtg gaacatcatt atgtggagcc ggaattcatc gattctgtct tggaaagaga
     9121 cagtcactcc agtactagag tagcaccatt tgtaacaatc cctcacggta atccgctgta
     9181 tgtgaagcat tcgatgattt ctattgctac aatgaaagaa ccagtcctat ggcacgggga
     9241 acagatacgt atcgtactgc tattagcaat aaagaaaaat gatttgaaac atgcagagtt
     9301 caaaaaaata ttttctgtta ttcactatct agaaaagcag ccagaacaaa tggctcagtt
     9361 gatgcaatcc aatcatccgc tagaaatctt aacgttgtta tctagttatg aataagtagc
     9421 gattaaaaaa ctgcctttat atttataata attctatcta tttatttgga agtagaaaac
     9481 taaatacaaa aaaagaggat gtggccaaag ttgtggcaca tccttttatt ttgagtttct
     9541 tataattttt tatagttgct tacgatgtct tgactactgg taaacggtgt tcaagagaca
     9601 atttgtcggc aataaggcac aattttgtaa aatatggaaa gctatttttt taacattgtt
     9661 tcttagtgct tgaaaaatca ttttgaatac ttctttatac gatcgatgta cgttgattta
     9721 agtctaaaac ttttcttttg attccttctc tgacttttct gaattccgtt aatatttctt
     9781 cttcgctccc gacagcttgt gcaggatctt ttaggtccca gtgaaggtgt tcgtgattgt
     9841 ttggaacggc agggcacttg tctttggcat caccacaaag tgtaatgatc aagtctgcct
     9901 gagtaaagta atctctatca atca
//
LOCUS       DAGCYL010000101         9896 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.102, whole genome shotgun sequence.
ACCESSION   DAGCYL010000101 DAGCYL010000000
VERSION     DAGCYL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.102"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..888)
                     /locus_tag="MB216_001877"
     CDS             complement(<1..888)
                     /locus_tag="MB216_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBT4526681.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLI"
     gene            1034..1837
                     /locus_tag="MB216_001878"
     CDS             1034..1837
                     /locus_tag="MB216_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBT4526682.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2015..2962
                     /locus_tag="MB216_001879"
     CDS             2015..2962
                     /locus_tag="MB216_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBT4526683.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(3030..3323)
                     /locus_tag="MB216_001880"
     CDS             complement(3030..3323)
                     /locus_tag="MB216_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBT4526684.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3351..4016)
                     /locus_tag="MB216_001881"
     CDS             complement(3351..4016)
                     /locus_tag="MB216_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBT4526685.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4156..4788)
                     /locus_tag="MB216_001882"
     CDS             complement(4156..4788)
                     /locus_tag="MB216_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4526686.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4795..5862)
                     /locus_tag="MB216_001883"
     CDS             complement(4795..5862)
                     /locus_tag="MB216_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBT4526687.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(5859..6590)
                     /locus_tag="MB216_001884"
     CDS             complement(5859..6590)
                     /locus_tag="MB216_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBT4526688.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(6758..7237)
                     /gene="greA"
                     /locus_tag="MB216_001885"
     CDS             complement(6758..7237)
                     /gene="greA"
                     /locus_tag="MB216_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBT4526689.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7373..8548)
                     /gene="mltG"
                     /locus_tag="MB216_001886"
     CDS             complement(7373..8548)
                     /gene="mltG"
                     /locus_tag="MB216_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBT4526690.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            8713..9873
                     /locus_tag="MB216_001887"
     CDS             8713..9873
                     /locus_tag="MB216_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBT4526691.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT         2991 a         2032 c         1554 g         3319 t
ORIGIN      
        1 aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat gtgtaatacc
       61 atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct cgcttagtag
      121 ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat actgccaaat
      181 atggacatga tgaacattct ttaccccatc gattgcttca atttcttgtt ccattttttc
      241 atagtcaata gcaggtgcac tttgtatcaa aatagcggca gcttttttga tcaccattat
      301 cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga tccagtagat
      361 atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca gtgtatcact
      421 taacaaatgc aaataactag attttatatt caaactttca tctgcttctt gtcgtaataa
      481 taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca tcaaatgact
      541 atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa acaaggataa
      601 tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct ggtagccata
      661 cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag aaaaaataat
      721 tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac tccctgacat
      781 aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta ctccaaatag
      841 tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt tatttatatt
      901 ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt cagatgtttc
      961 cctaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg acactactag
     1021 atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac gattcaaaga
     1081 cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt tagttttgag
     1141 tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaata acattcaatt
     1201 ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg ggcagcttca
     1261 aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca catttgatcc
     1321 agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt ttgttagtgt
     1381 cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga cttctgcttt
     1441 gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa acttaacagg
     1501 taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa aagtcctgac
     1561 atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc tatttaccaa
     1621 cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc ttgattgttt
     1681 aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct tgatggtctt
     1741 tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta tttttgctca
     1801 agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa ctcgttattt
     1861 ctacttaaat agaccctttt agaggttgtg acattttttt gtcacagccc cttttttatt
     1921 tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc ttgtatgcgc
     1981 tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca tttatgcaga
     2041 tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg gttatatctt
     2101 taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg gctggaactc
     2161 agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat ctatctcagc
     2221 tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac tttcaaatgg
     2281 tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc ttgggtattc
     2341 tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag gttcatcggt
     2401 ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc atattggaaa
     2461 aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca atacatccgg
     2521 tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg aagttcttcc
     2581 tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg ggctctttga
     2641 tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg gccgtggtgc
     2701 ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt cctttgcagc
     2761 attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga aaatggacaa
     2821 tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga aattaatggc
     2881 tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa ccaatcaagt
     2941 aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa aaagagaagg
     3001 tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata ggttggctcc
     3061 tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca ttcatctgct
     3121 tcctcgataa aaaaaagtgt cccatccatt tccactttct ttacaggatg ttctggctgt
     3181 tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta gacttttcca
     3241 aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca tttttgtgct
     3301 tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt ctattgattc
     3361 atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag attatcattt
     3421 tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac tcttcgtatg
     3481 gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt tttgttgaaa
     3541 aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacag ctctaagtag
     3601 tctagaatat tctttgatac taagttatgt gcaatccgta tccccatgtc tcgctcaggg
     3661 tgtacgacac gatctgctcc gatcttctct agtacacggg catggtactc attttgggct
     3721 tttgctaaaa tattcggtac gcccatttcc tttaccatca gagtaactaa gatgctggct
     3781 tggatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatacc taatgaacga
     3841 agtgtcatct catcctgtgc atttgccaca acagcatggg tggctatatt catatattca
     3901 ttgactcgat cttcattact gtcaatagcc agtacttctt gtccagcttc gatcagtgta
     3961 cggcagatgc ttccgccaaa tcgtccaagg cctacaattg caaaattttg tttcaccatt
     4021 ttcctccttt agtatcttct tttctcagta taacaaagcc tttccaaaaa caaaagcaag
     4081 catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt ttgattatcc
     4141 tctttatcat cgtttttatt tcaccaaacc atgtttaaac gcatagatag ctgcttgagt
     4201 tcggtcttct acctctaact tagctaatat attcgacaca tgcgttttga ctgttttcag
     4261 tgtgataaac aattcatccg cgatttcctg attacttttc ccttcagaaa tcagcatcaa
     4321 aatttcattt tctcgattcg ttaattcttc atgcaatact ggatcatttc gccgactcat
     4381 tttttccatc atttttgttg tcacttcagg ttctagtact cgctcgccgc gttgcgtagc
     4441 ccttatagca tcagctattt catgtgctgt agacgtcttc aacaaatatc ctgctgctcc
     4501 tgcttcaatt gcaggataaa ctttttcatc atcaataaaa cttgtgacga taatgatttt
     4561 ggcttttggc caatctttta agattgcttt tgttgattcg atgccatcca tttcttccat
     4621 gactagatcc attaaaatga catccggtcg aagtgccatc gccttttcat agccttgacg
     4681 tccattttca gcttctccaa tcacttcaat gtcttcttgg atagataaat aagaagagac
     4741 gcctaagcgt accatttcat ggtcatctac taataaaact tttatcatct ttatctatgc
     4801 ctccttcatc aaagggacct tggtctccac gctcgtacct tgccctttaa aactaatgat
     4861 ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc cataacttcc
     4921 cgttttggtt tcattcatat taaatcctgt cccgtcgtca atgatacgta ataaaagatt
     4981 gttatctatt ttatgcaaat agacttctaa ttcattagcc ttagcatgtc ttaatgtgtt
     5041 tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag ttaattttac
     5101 atcttctacc tcccatttta gcgaaatctg gattttattc tgcaattcct tcaataactg
     5161 ctcaatgcct tgttttaaac ttttttcttc taaattgact ggacgcagat gaagaagcag
     5221 cgcacgcatc tcagactggg aagcgttgat gatctctgcc accattttca actgcttacg
     5281 gaacatttct ggtgtctcac ttttttctgc ttgctccgtc aatgccgaca tcatcatcat
     5341 cgctgcaaat aattgctgtg aaactgaatc atgaagctca cgagctaatc gatgcctttc
     5401 taattttaaa atctcttctt tggtttgtcc atctacatat tgaggacggc tagtcataat
     5461 ctgtaattca ctggatactt cgatcatttt ttcttttatt ttagtaattt ccatatcaat
     5521 atctttgatg tacagatcat ccgacgcgct tgggatcggc tgatgcaaaa gtttggattc
     5581 ataattgcca gaagaaagtg cacgaagctt ttcttcgatt ttcccgtatt ttgccttttg
     5641 aataagtgat aacaatagaa atacgatcaa tcctacacct aatgaaattg ccatcaaatg
     5701 gaagataagc ggaacgtata ataaacgcgc acgcagcaat tctccccacc aatgactttg
     5761 gttactggca taaaaataag tgaaaagtga aaatagaata ataaggaaag ccgcaatccc
     5821 cgaataaaca gctagcattg ctttggatat tcttcccatc atattcgtat cacctcgaca
     5881 tctccaacca aagtattggt aatgatcttc aatctgcgag gattttcatc ataatcactg
     5941 ctgcagattt tgatctgttc gttttttaag gaaaactgct cttcttcaaa caagacattt
     6001 cctactaatg tcgcatgctc taaacgaata gccactccca ggggtactaa gatccgtgtt
     6061 ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag tgtattgccc
     6121 aaatcgatga tagtatcacc agaaatgatg gcaatgttga tatcatccca ttcatagacg
     6181 tcgtttccaa tacgttggtt accaaataat tgctgtcttt tacgctcatt attatgtgtt
     6241 tttaattgct ctgtttggac catcatgatc tgtttttttc gccaaaaagc attttttgtc
     6301 aaatctattc ctgaaatctc tacacctttc agcccgataa ataaaacggc caatacaacc
     6361 atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaagct ccctaagatc
     6421 aattgaaaat tttgaaattt agtacgagga tatttgcgca tagctagata tatattgaaa
     6481 ataccgaata ttactaaaag caaaagtcca gtattgttta cgatttgcca aacagcaaat
     6541 aacagcagca gtgcttcgac taccacaaaa aaacgccaag aactattcat cgctttttct
     6601 ccctcctgac acattctcat ttcatcatac ctattttttt gactgttggc tataggactt
     6661 tagatgtaag atcgttcagt gaaacctgca atactcaaag agaaaagatc tagagtgcgt
     6721 catcagtaag atgaactctc tagatctttc agtcagacta cgcttgttct actttgacga
     6781 ttttaactaa catatcgcca cctggtgtag caatggttac ttcatcattt ttacgtttgc
     6841 caatcaatgc ttgcgcgata ggagaatcat tggaaatctt tccagaaaac gggtctgctt
     6901 cagcacttcc gacaatcgtg tactcttctt catctccatc aggcaactcg atgaacgtaa
     6961 ctgttttacc gatcgatacc tcatctgcat ctacgccgtc attatcgatg atttgtgcaa
     7021 aacggatcat attttcaatt gttgtgattc ttccttcaac aaatgcttgt tcatctttag
     7081 ctgattcgta ttcggagttt tcggaaagat ctccgaaacc gcgagcgatt ttgattcgtt
     7141 caatgatttc tccgcgttta actgttttta gttcttctaa ttctttttct aatttttctt
     7201 taccttctag agtcattgga taaactttct ctaccatgtg taacgtctcc ttttttcgat
     7261 aaaataccgc aaaaggtaat cttttgagga atatagtcca aaaaagatta ccacgctttt
     7321 cttaaaatgt aaatgttttt ctaaaaatga tcaatgacta gcccctgttt ttctaagaat
     7381 tatttacata tttttgcact agttccatat gttcttcata cgttttagcg aaataaacat
     7441 ttccagtgct cgtatctgct acgaaataat aatagtcatt ttctgctggt tctaatactg
     7501 ccttgattgc ctcttcgctt ggattgtcaa aaggaccagg cccatagccg gtattggtat
     7561 acaaattgta tggtgaatct acagctgtat cttcatacgt caccaattct ttatgttctc
     7621 ctaatgcata taaaacagag atatctgact gaagtggcat atttgccttg attcggttaa
     7681 agaagacttg tgcaatattt ttacgatcat tttcttttac gccttctttt tcaactagag
     7741 aagccaaagt taaaacttct tgtacagtca aatttttctg tttgatagct gaataatagt
     7801 tttgcatgac cgtatttgtt ttgttcacca tctcaatcac aagatcctta agcgaagtac
     7861 tcgtgtagta atcataagta gctgggaata agtagccttc caaacggtat tttgtgttct
     7921 cagctttaga ggcactttcc aacaattctg ggaatttttg atacaactcg ttaaagaaag
     7981 tctcatcatt tactaaatct aaaaattctt tcttgtcttt accagttacc ttcgctaccc
     8041 gctcagcaat ctgatcgatg tcgtagcctt ctggaatggc aattttccca tctgcaactt
     8101 ttgttggctc agaagtgccg ccttcttgca gctgtttgcc aatctcgtca agagtcatat
     8161 taggcgctaa ctgataatag ccagcttgaa agccagttaa atttttaaat tttgtataat
     8221 aattgaaaac aatcccgctt ttaataatat tgtccttctc caaaatctca ccaatctgct
     8281 tgttagaaga gccactcggg atctccactt ggactaattg gtcatctgat ttatctaaag
     8341 gcttcaaccc gctatctaca taacgataca cggtaaatcc aagaaatccg ccaataatta
     8401 ataatgtaag agcaatcact aaaatgatct tcctcacaat cttatcttct tttttacgca
     8461 gatttctccg cttgtcgatt atatctgctg tagctgcctt actggcttct tttttatctt
     8521 tacttgggtg ttgtttgttt cctgacaaaa caactcctcc ttactatcat cttcgtaact
     8581 ctgtttatta tacatgaaat ttctactgcc gtgaattatt ttaccaaata ttatgtcaaa
     8641 attgtaagtg gcaaatactc tttttgagga ttacaatgaa aaatagttaa aaatggatag
     8701 aaaggaatta acatgtcaaa aggaaaacat cgaaaaaaac aaaaaatttc tatttctttt
     8761 cttgtaattt tattattgtc tgtcagcttc ttttcaacag gcatcattat aggaggtcaa
     8821 ttaacaaaaa tacctcattc ccaaacttat aaagcggagc caacacaact tacttccaat
     8881 ttaacgcccg atgagcagag gcttgctagc aaaattgatc aattactaca aaaatcagat
     8941 tatatcggtt cgatttatgt tcgcaaaaat aatcgcgtac tattagaaaa aggatatgga
     9001 tatgcaaatc tgcgcgtaaa agaatcgaat tcaccttcta tttactatca aatcggttcg
     9061 attcaaaaag caatgactgc tttactgata ctaaaacaaa tccaagctgg taagttaact
     9121 tttgacacaa agttagcaga attttatcct cagattcctg gaagtcagca aatcacaatc
     9181 aaaaatatgc tttatatgcg ctctggtctt catcgaactg cttctcccga caaacctatg
     9241 tcggatagtg aaatcattca gttcgccctt catcatttga aacttactga ttatgatacg
     9301 tatcattatg aaccattgaa ttatacattg cttacaggga tattgatcaa actaacgaat
     9361 cagccttatg aaactcttct aaaaaaagaa atcattcgtc cactccatct agaacataca
     9421 gatttttatg acaatgtaaa aaattcgcct caacatgctg tatcttacca gatgtctgca
     9481 actgacgatt attcaaaagc attgatcgag cctgaaaccg acatccgaaa tgaattaggt
     9541 acaggaaaca taagtatgtc tgtttatgac ttgaacaaat tctttaccag tgttctttct
     9601 ggcggtatca taccaaaaga actacttttc tcattgtggc aaaataatgg tgataaacat
     9661 ccttacagcg gcggtgttta cagtggaaac gattatatcc ttgctcaggg gaatatcaat
     9721 cgattccatg cagtagtggc gatgaaaaaa gacactaaag atgcaatcgt catggaaagt
     9781 aatatccaat cggataagaa catcaaattg ccggccactg atttaagaaa tcagatttat
     9841 gaactgatgg aaggtgttca cttatcaaat taaattttga cataatatta gttgtc
//
LOCUS       DAGCYL010000102         9812 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.103, whole genome shotgun sequence.
ACCESSION   DAGCYL010000102 DAGCYL010000000
VERSION     DAGCYL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.103"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(33..2153)
                     /locus_tag="MB216_001888"
     CDS             complement(33..2153)
                     /locus_tag="MB216_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBT4526692.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2383..3561
                     /locus_tag="MB216_001889"
     CDS             2383..3561
                     /locus_tag="MB216_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBT4526693.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3577..4335
                     /gene="aroD"
                     /locus_tag="MB216_001890"
     CDS             3577..4335
                     /gene="aroD"
                     /locus_tag="MB216_001890"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBT4526694.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4358..4843
                     /gene="ybaK"
                     /locus_tag="MB216_001891"
     CDS             4358..4843
                     /gene="ybaK"
                     /locus_tag="MB216_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBT4526695.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4914..6167)
                     /locus_tag="MB216_001892"
     CDS             complement(4914..6167)
                     /locus_tag="MB216_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4526696.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6284..7633
                     /gene="gdhA"
                     /locus_tag="MB216_001893"
     CDS             6284..7633
                     /gene="gdhA"
                     /locus_tag="MB216_001893"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBT4526697.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7745..9091
                     /locus_tag="MB216_001894"
     CDS             7745..9091
                     /locus_tag="MB216_001894"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBT4526698.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9399..9785)
                     /locus_tag="MB216_001895"
     CDS             complement(9399..9785)
                     /locus_tag="MB216_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBT4526699.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2010 g         2892 t
ORIGIN      
        1 attttgtcgc tgctcctttt aaatttattt tatcattcgg aagaacttgt ggtcgttccg
       61 tgaagttctt ggtacgtttg cgttttataa agatcaacag ttgattgtcc gttgtgttca
      121 ctaaagacac tagttgactt gtcaccaaga gatttttcag cttcttttaa tttctctaag
      181 ccattcttgt agtcataatc actggtatca attgggtcta atccactatt cgtatagaac
      241 cgaagaagat caccattatt gatttggtca gaaacagcta actgtttatc aactttttct
      301 ttcagtgaat caatttcatt ctgtacttcc tcagtaggtt cagtaatagc ttcaccagtt
      361 tcgttactat agatcgtacc accgtagtaa gtatattttg gtgtaacaaa atcaccatca
      421 cggaaagaaa caagctgttc atgatctttc gagaatagat cttgtcctaa ctgaatataa
      481 ttttttgtat ctacacctag taagtgaagt aatgtaggca aggcatcaac ttgtccgccg
      541 taagtgtgat tgattccgcc ctgtgtttgt ccagggatat ggatcatata agggacacgc
      601 tgcatttgtg cattgtcgta gtttgtccaa gtagaacttg tttttccaac taattcagct
      661 agatttttat ttcttgaatt cgagacaccg taatggtcac cgtaaaggac gatgactgaa
      721 ttatcataca agccgctttc tttcaaataa ttgaagaatt cttgtacggc ggtatctaaa
      781 taattggctg tcgcaaagta accgttgatc gtttcatcat ttgtattggc taacgggaat
      841 cctgcttcgt cattggtgaa ttttgaataa ggataatggt tagatacagc aatgaatttt
      901 gaataaaatg gttgttgcag acgttccaag tattgtacgg attgttggaa aaatggttta
      961 tcatgcaacc cgtattggaa cgaattgtca tcattaacat cataataaga agaatcaaag
     1021 aaataatcat atcctaaatt cttgtatgtc tcgtttcgat tccagaatgt accagcgttc
     1081 ccatggaata ccgcactaat gtaaccttgt gtttgactta aaatatcagg tgcagcttgg
     1141 aaagtatttt ttccgcctaa ttgtgtgaac aaggagcctt gattcaagcc aaacaatgaa
     1201 ttttccatca gcgtttccgc atcgcttgtt tttcctgctt ttacttgatg gaaaaagttg
     1261 tcaaaactga acgtgctgtt gctgtggtaa agactattga tgaaaggtgt aacttcatgc
     1321 tggagaccat tctcatctgt caatttataa tcgatcagga attgctggaa gctttctaaa
     1381 tggatataaa tcacattttt tcctctagcg agcccgaaca aatcgtcatt tggtgctgca
     1441 taatgttctt ttacataatt ttctacttct ttcatatcat tgggacttgc ttctgcccgc
     1501 acttggtttg tctggtaagt ttgaacaccg tcataaacca tgaaagcatt caagccaaga
     1561 tatttgacca aataatcacg agagaatgtt cgagtcagca attctggacg atcagcttcc
     1621 gccataaaaa gatttcctga gaaaatcata actgctaatg tagagatcgc aaaagccatt
     1681 cttgctcgga tcgggcgagg gtccaacttg attttttttg caatcagcag aacagtcaag
     1741 ataataaaat cgatccaata gatcacatca tatgggcgaa acagtcgaat ggcactttct
     1801 cccagaccac tcgctacttt cccagcacct aaaatcgtgt tgatcgtaat gaagtccgtg
     1861 aattctcgat aataagtgac attcgaaaaa acaagcaatg tcaaaagaaa ataaatgacc
     1921 atcatcgtga tataagcaac tctagggcgt ctgatataaa gagcaattcc aagtaataac
     1981 aatgtagttg caatagggtt gataaacaag ataaagtatt gaatgccact ttcgattccg
     2041 agatggaaat caactaaata ggcaaaaatc gttttcaacc aaagtaaaac gactagtaaa
     2101 gaaaaaaatc cgagcctctt attcaaaaag gcaggtgttt ttatacattt caagataatc
     2161 ttcctttctg cacttcttaa tcacagaagt gtcctgaaac catgttacac agctcactta
     2221 ttctcattta ttttactagt atgacaatat agtgtcaatt gaaaccagaa gtttatgctg
     2281 tttcattaag gttttcttta agcttcatga aaaaatgagc agtaagacaa gtcctatgtt
     2341 ttttgttata cttgggaaga gaaagctaaa gcaggtgaag gaatgaaagt aaaagtaaaa
     2401 aaaagagcaa gcaatcgatt gaaaaaagga tatccactga tccaaaaaga agaccttgct
     2461 catccccaaa aagaaaccac gcaatgggtg gaattagttg attcgcaaaa ccaatttatc
     2521 gcaaaaggat atctaggaaa gcagaataag ggaatcggtt gggtattgag ccagcagaac
     2581 atcccatttg atcaatcttt ttttgaagaa ttttttgtga agtcaaaaga aaaaagaact
     2641 ttcttttttt cagatgattc tactaccgct tttcgtatat tcaatggaga aggagacggg
     2701 atcggcggtc tgattgttga ttattatgca ggttatgctg ttttttcttg gtataacgaa
     2761 acgttatatg cacatcgtga attattgatt caagcttttc gtgaaatcta cccggaaatc
     2821 ataggaggct atgagaaaat tcgatttgaa tcagtaaatc tgcctgagag ccagttcatt
     2881 tacggaaaag aggcaccgga acccttgatc gtaaaagaaa acggagtaaa atttgccgtt
     2941 tatttaaatg aaggattaat gactgggatc tttttagacc aaaaagaagt acggggatca
     3001 ttagcagaag gtttcgcttc tgggaaaaca gttttgaaca tgtttagtta taccggcgcc
     3061 ttttctgttg ctgccgcaat gggaggggca atcgaaacaa caagtgtcga tctagcaaaa
     3121 cgcagtttaa aaaaaacaca ggaacagttt gaagtgaatg gtttggatgt cgatcagcaa
     3181 aaaatcattg ttatggacgt attcgagtat ttcaaatacg ccaaaagaaa acaattttta
     3241 tatgacatga ttgtattgga tccaccaagt tttgccagaa ataagaaaaa agtctttcgc
     3301 gtagcaaaag attatgggga actggtcaag gatagtctgg atattttagc agaagacggg
     3361 atacttattg cgtctacaaa tgctgcgaat gtctcgatag aacaattcca aacaatgatt
     3421 gaagaggaat tccatgccaa aaatgtttct tttgaacaac ttgctttata tcgtctgcca
     3481 aaagatttcc agacgacgga taccttttta gaaggcaatt atctgaaagt gtttgtttat
     3541 caaattaaaa ataaccatta gaaggagcga tgcagaatgg ttcgggtaaa gggattgaat
     3601 ataggaagcg gtagaccaaa gatttgtgcg cctcttatcg gggccgtccg agaggaagta
     3661 ttacgtgaag cagttcttgc acgtgaagca ggggccgatt tagtcgaatg gcgagtcgat
     3721 cattatcgtg aagttttttc ttttgatgaa gtagcagaaa cattggttct tatccatgag
     3781 atgcttgatg gactgccgtt attatttacg tttcgtacct tagctgaagg cggagaacaa
     3841 gacatatcga tccgtcagta tcgcgacctt tatgaacatg tgatacacac aggattagta
     3901 gatatggtcg atatcgagtt gttcaaagta gaaagtctag gaaaaagact gatagaggaa
     3961 atcaagtctt taaacattcc tttaattatc agtagtcatg attttaaaga aacaccagca
     4021 gaccctgtct tgctttaccg attgaatgtt atggaacatt ttggagccga tatcggaaaa
     4081 ctggcagtaa ctcccaataa tgaacgagat gttttgcggt tgatggagtt gactagacgg
     4141 gcaaatgctt ttgtctccat gcctattatt acgatgtcca tgggaaatct tggtaaaatt
     4201 tctcgtctgg ttggcggaac gacgggttcc gttatgacat ttggttcctt aagcagcgat
     4261 tctacttctg ctccaggaca attaccggtc aaagcattga agcaaattat tcaattatta
     4321 aatgaaccgt tgtagaacaa gtttgaggtg atattttttg gcaaagaaaa aaatagtaaa
     4381 aacgaatgca atccgtatgg tcgaacaaaa aaagattccc tatacagagc acgaatatga
     4441 gtgggacgaa agccacctaa gtgcgtcatc ggtcgctgaa cagcttccag aaagccaatc
     4501 tcgtatattt aaaacactgg ttgctgtggg caatgtgacg gggcctcttg ttgctgtgat
     4561 accgggagaa gccgaattga atctaaaaaa attagcaaaa gtttcaggga ataaaaaagt
     4621 agaaatgctc catttaaaag atttggaagc aacgaccgga tacatacgtg gtggctgctc
     4681 gcctataggt atgaaaaaac tatttcctac ttatttagat caaattgcag aaagctatga
     4741 acaaatcatt gtctcggctg gcagaagagg gctacagatg gagttagcac ctcaagatat
     4801 ctgtgcatta acgagcggac aatttgcaga tataaaacaa taaatcatac gtataaaaga
     4861 aagaaggagg ccgggatgtt gtttgtccag tctccttctt tcttttttat acattatgct
     4921 ctttgcccgg cattcattcc tgctacatgc cctgtagcga aagccgctgt gatattaaag
     4981 ccgccagtat aaccattgac atccagtaat tctcccgcaa aatacagacc atcaatgatt
     5041 ttactctcca atgttttagg ataaatttcc tttagagaaa cccctccgcc agttacaaat
     5101 gatttctcaa taggaaaagt tttgttgatt gatagcttaa actctttgca tagttgaaca
     5161 aaatcttgaa tttgtttttg agaggcttgt tgtcctgtca gatgatccat ttcaagccgc
     5221 tcaagaaaga aaactaacaa tcgctcaggc aatataccat gccaagcatt gaccaagttc
     5281 tttttggttg ctttactttt ttctgtcaat atatgaataa gctcttgttt ggtttgtgta
     5341 gggaaacaat ccagagaaag tgtcactggt tctcccgttt tctctaattc tttattgaca
     5401 aaacttgaac aacgcagtgc agccggacca gaaataccga aatgggtgaa aagtaaatcc
     5461 atcgtatgtt cagtcaatac acgacctttt ttatttaaaa cgcgtaaagt gatgtcttgt
     5521 aaagaaagcc cttgaagtgt tttctcctga atatacggtt catccgatat caagggggac
     5581 tctgtcgcat aaagaggcgt caccgtgtga cccactcgct tcacgatctt gtacccatct
     5641 ccagtagaac cagtagatgg ataagtccgt ccaccagttg ttaagataat ggcaggagca
     5701 tggaactctt cgaattccgt gcgtataccg taaattttat gttctttgtg gatcaatttc
     5761 tctactcgag tagagaatag aagcgtcaca tctagttctt tcagacgatt gagcaatgct
     5821 tcgataatcg tttttgattt atttgttaca ggaaacatcc gtccatgatc ctcttctttt
     5881 aaatgtacgc cttgtgattc aaagaagttc atgatatcga aattattcca ttgcgcaaac
     5941 gtactatata aaaactttcc atttccagga atatgtgcaa tcaaatcatc aacaggacga
     6001 ttgttcgtaa cattgcaccg accaccacct gtcatcagta gtttttttcc tgcttttttg
     6061 tttttttcga tcaagagtac gcgtgcgcct tgttcagctg cactgatcgc tgccatcatc
     6121 ccgcttgttc cagcgccgac tacgatcaca tcatacagtt tttccattcc ctcacctcaa
     6181 ggaaagtgta gcatatctga agaaagaaaa cagaaaaaaa tgttttgagc caaaagagaa
     6241 aatgagctaa aatgagggaa gataagcaaa ggagcgtgga acgatgacaa acgcaacaga
     6301 atatgttcaa gcaattcaag aaaagctaca tcaaaaggat caagggcaag aagaattttt
     6361 acaagcaatc gatgaattca tgcccacggt tatgtctttt ttggacactc atcccgaata
     6421 tatcgaaaag aatatcttag aacttttaac ggaaccagaa agagttattc aatttcgagt
     6481 gccttggcaa gatgatgaag gaaactggcg agtaaataga gggtaccgta tccaatacaa
     6541 ctcagctatc ggcccttata aaggtggatt gcgcttccat cctagtgtca atctaagtat
     6601 cttgaaattt ttggcttttg aacagatttt caagaatagt ttaaccggtc tgccaatcgg
     6661 tggaggaaaa ggcggaagtg acttcgatcc taaaggaaaa tctgataatg agatcatgcg
     6721 tttttgtcaa agtttcatgc tggaattgtc aaaacatatc ggcccatcga ttgatgttcc
     6781 tgcgggagat atcggtgtag gagctagaga aatcggctat ttgttcggcg aatataaacg
     6841 attgaaaaaa tatgatactg gtgtgttgac aggtaaacct ttggattttt ggggaagtaa
     6901 gatacgtacg gaagctactg gttatggatt agtgtattat gtcaaacatc tattgaacga
     6961 agaaaaagat tcatttaatc aaaaaacagt ttttgtttcc ggaagcggga atgtagccat
     7021 ttatgcaatc gaaaaagtgc aagagctagg cgggaaagtt gttacatgtt cagattctag
     7081 cgggtacatc tatgatccag aaggaataga tgtgaaacta ttgaaagaag tcaaagaaat
     7141 caatagacaa cgtttgacgg aatacgcaaa ccaacgtcct tctgctattt atcatgctgg
     7201 agaatctgtt tggacattga aagaaaaagc ggatatcgct ttgccatgtg ctactcaaaa
     7261 tgaaatagac gaaagcttgg ccgaaatctt agtagaaaat gggatcaaaa tcgtagcaga
     7321 aggcgctaat atgccatgta cagtaaaagc tgtttctgtt cttcatgaag caggcgtgtg
     7381 gtattgtcca ggaaaagctg ctaatgctgg aggagtagca gtatctgctt tagaaatgag
     7441 tcagaacgtc cagcgattga cttgggaaag agagcaagtg gatgagcagc tggatcaaat
     7501 catggaaacg atttaccata cgtgtcgcca aacagcagcc gagtacggcg acgaaaaaaa
     7561 tctgcttctt ggagcaaatg ttgctggatt tgaaaaagtt gccaaagcaa tggcagcaga
     7621 aggtttagtt tgaaaaatga aactttcttg gaaatattgt cagcattttg tttatttgct
     7681 tgaaaaataa taaaaaaaaa cgtaaagtat tacctgtaga gtaaacctat aaggaggaga
     7741 acaaatggca cacattcatt ttgactattc aaaagtagca ccgtttgtca acgaacaaga
     7801 gttagggtac atgcaaagcc aagtaacagc agcccacaac gaattacggg aaggtactgg
     7861 cgcaggaagc gacttccgcg gctggatcga tttaccaacg aactatgaca aagaagaatt
     7921 tgcacgtatc aaagaagcag ctaaaaaaat ccaatctgat tctgaagtat tagttgttat
     7981 cggtatcggt ggttcttatt taggcgctcg tgcagcgatc gacttcttga atcacacatt
     8041 cttcaattta ttagatagca acacaagaaa agcaccacaa atcttctttg ccggaaattc
     8101 aatcagctca acatatattg ctgacttgat cgaagtaatc ggtgatcgcg acttttcagt
     8161 aaacgttatt tcaaaatctg gaacaactac tgaaccagcg attgcgttcc gtgtattcaa
     8221 agagcttctt gttaagaaat atggacaaga agaagcaaac aaacgtatct acgctacaac
     8281 agataaagca aaaggtgcag ttaaagtaga agcagatgca gaaggttggg aaactttcgt
     8341 gattcctgat gacatcggcg gacgtttctc tgtattaact gctgttggtt tgttaccaat
     8401 cgctgttagt ggtgcagata tcgatgcatt gatgcaaggt gccgctgatg caagcaaagc
     8461 atactcaagc gacaaactag aagaaaacga agcttaccaa tacgcagcaa tgcgcaacat
     8521 tctttaccgt aaaggcaaag taactgagtt gttgatcaac tatgaaccag gaatgcaata
     8581 cttctcagaa tggtggaaac aattgtatgg tgaatctgaa ggaaaagacc aaaaaggcat
     8641 ctatccttca agcgcaaact tctcaactga tttgcactct ttaggacaat tcatccaaga
     8701 aggacgtcgc aatatttttg aaacagttgt gaaggttgaa aaacctcgta aatcaatcac
     8761 aattcctgaa caagcagaag atttagacgg tctaggctac ttgcaaggaa aagaagtgga
     8821 cttcgtaaat acaaaagcat ttgaaggaac attgcttgct catacagacg gtgacgtgcc
     8881 taacttgcta gtcaaaattc cagaaatgga tgcttataca ttgggttata caatgtactt
     8941 ctttgaaatt gctgtaggta tttcaggtta cttgaatgga atcaacccat tcgaccaacc
     9001 aggtgtagaa gcttacaaga aaaacatgtt cgcacttcta ggcaaaccag gctttgaaga
     9061 tcttgctaaa gaattgaacg aacgcctgta agataagggt ttttcgctag atttaaataa
     9121 tcagtgatag aaacatgttc gctttaaaat gaaaactggt acccatttag gtactcaaac
     9181 atactgcatc gtttattcag aataaggggc tgtgacaaga cgtttgtcac agtcccttgt
     9241 ttgtatagaa atcgattgat tgccttgctc tttagtttgt tttaggactt taagaacagt
     9301 gaggctgact tcgaaggtta attctgtttt tttgttttct agaatcagta agcaaaaaag
     9361 cactcacttc gagtgctttt ttgcgatata ttcttagctt aagataggcc taatttttct
     9421 tttacagatg agggtacctt atcgctggta attacttttt tctctatgat ccgcttttcg
     9481 cttgctttca actgcaagat cgttccttcg gggtaagcag aaccattggc agagtcacca
     9541 gaagtaatga catcgaaatc taactgtctt ttttcaccgt tttcagcata agcatctacg
     9601 ttgtatttaa aacctcttcc aattacttcc ccattatctg ctttgatatc agtttctgca
     9661 ggcattggag ctttgactac cgcataataa tctttaccta cataagtaga ctgatagtag
     9721 tcccaacctt tccatcctat aaaagcaatc aatatgataa cgatcaaccc taaaattttt
     9781 ttcatgaaag atctcctttt ttatttttat ta
//
LOCUS       DAGCYL010000103         9653 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.104, whole genome shotgun sequence.
ACCESSION   DAGCYL010000103 DAGCYL010000000
VERSION     DAGCYL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.104"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            4..741
                     /locus_tag="MB216_001896"
     CDS             4..741
                     /locus_tag="MB216_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBT4526700.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            755..1570
                     /locus_tag="MB216_001897"
     CDS             755..1570
                     /locus_tag="MB216_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBT4526701.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1583..2230
                     /locus_tag="MB216_001898"
     CDS             1583..2230
                     /locus_tag="MB216_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBT4526702.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2246..2902
                     /locus_tag="MB216_001899"
     CDS             2246..2902
                     /locus_tag="MB216_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBT4526703.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3064..3885
                     /gene="racE"
                     /locus_tag="MB216_001900"
     CDS             3064..3885
                     /gene="racE"
                     /locus_tag="MB216_001900"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBT4526704.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3888..5243
                     /gene="rph"
                     /locus_tag="MB216_001901"
     CDS             3888..5243
                     /gene="rph"
                     /locus_tag="MB216_001901"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBT4526705.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5244..5762
                     /locus_tag="MB216_001902"
     CDS             5244..5762
                     /locus_tag="MB216_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBT4526706.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5869..6369
                     /locus_tag="MB216_001903"
     CDS             5869..6369
                     /locus_tag="MB216_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBT4526707.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6649..7569)
                     /locus_tag="MB216_001904"
     CDS             complement(6649..7569)
                     /locus_tag="MB216_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBT4526708.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7734..8747
                     /locus_tag="MB216_001905"
     CDS             7734..8747
                     /locus_tag="MB216_001905"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBT4526709.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(8857..9612)
                     /gene="fabI"
                     /locus_tag="MB216_001906"
     CDS             complement(8857..9612)
                     /gene="fabI"
                     /locus_tag="MB216_001906"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBT4526710.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         3118 a         1699 c         2077 g         2759 t
ORIGIN      
        1 tctatggcaa tgattaaatt tgatcacgtg gataaatatt atggcaagtt ccacgcatta
       61 aaaaatatca atctcgaatt cgagaaagga gaagttgtcg ttgtcattgg tccttctggt
      121 tcaggaaaga gcacgatgct tcgctgtatc aatggattag agacgatctc ttcaggtaag
      181 ctgctgatta atgatactga cttgcatgat aaaaagacaa aactgacaga agtccgaaaa
      241 aacatcggca tggttttcca acatttcaat ttgtacccaa ataaaactgt tctggaaaac
      301 atcaaattag caccaatcaa agtgttgaag caagaccagg caactgcagt caaaaacgcg
      361 gagaaatttt tgaaaactgt caatatgtta gataagaaag attcttatcc ttccatgtta
      421 tccgggggac aacagcaacg ggtggcaatc gccagaggtt tagcgatgaa tcctgagatg
      481 ctgctgtttg atgaacctac ttctgctttg gatccggaaa tgattggtga tgtattagac
      541 gttatgaaaa aattggctca tgacggtatg tcaatgattg tcgtgacaca tgaaatgggc
      601 tttgccaaag aagtggcgga tcgtgttatc ttcatggctg atggagaagt attggaagac
      661 agcagaaatg tgagagactt tttcgaaaat ccaaatgaag tccgagcaca gcaatttatc
      721 agtaaagtta tcaatcattg ataggaggaa aaagatgcgc aggttaaaaa aaatacgcct
      781 gattttcctt ttaggcattg cttttcttgc actagccgga tgtaaaggaa atgggttggc
      841 aaatgaagat attttagagc gaagcaaatc taccaatgaa atcatctggg gtgtcaagta
      901 tgatactcga ttatttggaa tgatggatat tgagagcaga actgtacaag gatttgatgt
      961 agatattgca aaagcaatca ctaaaaagat ccttggtgac aatggaaaaa cagaatttgt
     1021 tgaagtcact tctaaaactc ggattccatt attgaaaaac ggaaatatcg atgcaattat
     1081 tgcaacgatg acgatcacag atgaacgaaa aaaacaggtt gacttttcag atgtctattt
     1141 cgatgctggc caagctttgt tggtaaagaa aggaagccaa atcaaaagtg tagatgattt
     1201 gaatgcttct acaacggttc tagcagtcaa aggttctaca tcggctgcga atatccgaca
     1261 gcatgcacca gatgcaaaaa ttttagaatt agaaaactat gcagaagcat ttactgcttt
     1321 acaatctggt cagggagacg cgatgactac ggataatgcg attttgttag ggattgcaga
     1381 tgaaaatcca gagtatgagt tagttggtgg aacgtttact aatgagcctt acgggattgc
     1441 gatcaacaaa gggcaagaaa acttcttgaa agctgttaac caagcgctag aggaaatgca
     1501 tgcagatggc acatacgata aaatctatca aaaatggttt ccaaatgaaa ctgaaggaaa
     1561 agtagagtaa gggggagagg caatgagcga attgatacaa acttatggac caatctttgt
     1621 cgacgggttc aagatcacat tgttttcgag tgtcttagca cttgtgttta gtttgattat
     1681 cggaacgtta atggcaatct tccaattatc aaagaataaa gtagtgagcg ccttagcaaa
     1741 ggcatatgta gaatttttta gaaacattcc attattaatc atcgtgatgt ttttttatgt
     1801 tgtcgtaccg atttattggt ttaatctaga tggattccaa gcaggtacga ttggattaac
     1861 gatctataca tctgctttta ttgctgaaac agtacgttca gggattcaaa ccgtaccaaa
     1921 aggtcaaaca gaagcaggac tatcttctgg ttttacctat tcagaaatca tgcgttatat
     1981 tgttttaccg caagcattca aaatcgttat cccgccatta ggcaaccaat ttatcaacct
     2041 cgtaaaaaac tcatctattt tagcaatcgt agctggttta gatttgatgt atcaaggaga
     2101 cttgatcgct agtgaaacat tcaatacctt cgatacgtat atcatcgttg ggttatttta
     2161 cttagttatc acgctaccgt tatcttactt aatggtttat cttgagaaaa aatgggcggt
     2221 tcgttcatag aaggagggga aatacatgga ttttagcggc gctttttcat ggatgaatat
     2281 ccgtttttta ttggaaggat taaaggttac tgttgaagtt tcattgtttt cgatcatttt
     2341 tagtttcttg atcggtggtc tgacaggtgt gattcgtttt gcgaatatcc ctttcttttc
     2401 caaaatcctt ggtgtagtga ttgatattat acggaatctt ccattattat tgatcatttt
     2461 cttcacttac tttgccttac ctcaaatcgg cattcagatg aacatcttct ggtccgctgt
     2521 cgcagcatta acgatttttg agtcagctat gttgtcggaa attttccgag ctggactcaa
     2581 tgctgttcca aaaggacaga tggaagcagg attatcaacg ggactgactt atgtagaatc
     2641 gatgcgaacg atcgtgttac cgcaagcatt taaatcaatg atcccggcaa ttgtgagcca
     2701 attgatttca ctgatcaaag atacttcatt agcagtaatc atttctttac ctgaactttc
     2761 tcatcaggca cgtattattt atggacagaa caccaattat gtgcttccga tgttcatcat
     2821 catgacactt atgtacttcg tggtatgtta cgcgctttct ctgctttccg gttacttgga
     2881 aaagagacgg tacagttatt aatagatgat tcttttagcg gttgtggcaa actaaagtca
     2941 caaccgcttt tcctctttcc attcgaaaaa aagagatttc gattgtttta agcaagtaag
     3001 agcacttgaa aattcacgct aaaaagtaca caatagggag gatagaattt attgaaaaaa
     3061 aggatgatac gattgacaga taatcgccct atcggattta ttgattcagg tgtcggcggc
     3121 ttgactgtag taaaagaagc cctgaaacaa ttaccgaatg aaaatatttt atttgtagga
     3181 gacacagcac gctgcccata tggccctaga cccgcggaac aggtaatcca gtatacttgg
     3241 gaaatgacgg attatctggt ggagcaagga atcaagatgc tggtgatcgc ctgcaatacc
     3301 gcaactgcgg tggctttaga agaaatcaaa gctgctcttt ctattccagt catcggtgtg
     3361 atccttcccg gtactagagc ggcagtaaaa aaaacacaaa ataaacaagt tggcattatc
     3421 ggtacgattg gtacggtaaa aagtcaagct tatgaaaaag cactgaaaga gaaagtacca
     3481 gaattgactg tgacaagtct tgcttgtccc aaatttgttt cagttgtcga aagtaatgaa
     3541 taccattcat cggtggcgaa aaaaattgtg gcagaaacat tagctccttt aaccactaaa
     3601 aaaatcgata cattgatttt gggatgcacc cattatccat tattacgccc catcattcaa
     3661 aatgtaatgg gagaaaatgt tcaactgatc gattctggag cagaaacagt aggtgaagta
     3721 tctatgctgt tagattattt caatttgagc aattcaccgc aaaatggtcg gacattatgc
     3781 cagttttata caactggctc tgccaaactt ttcgaggaaa tagctgaaga ctggcttgga
     3841 atcggacact taaatgtaga acatatcgaa ttgggaggaa aataaggatg cgtcatgacg
     3901 ggagaaaagc ccaagagctt cgcaaactgg agcttcaaac aaatgttttc aaatatccag
     3961 aaggatcggt agtgatccgt tttggtgata caacagttat ttgttctgca acaatagaaa
     4021 attctgttcc cccttttttg agagaaacag gaacaggatg ggtgacagca gaatatagta
     4081 tgctgcctcg agcaacgagt acgagaaatc gtagagaaag cagcaaaggg aaactttctg
     4141 gtagaacaat ggaaatccag cgtcttattg gtcgttcctt gcgtgcagta gtagacttgg
     4201 aaaaattagg agaacgtagt atcatcgtag actgcgatgt gatccaagcg gatggcggta
     4261 ctagaacagc aagtatcact ggggcatttg tggcattgaa attagccatt gaaaaacttt
     4321 taagagaaaa agaattgtca gaagatccaa taaaagaaca tcttgctgca gttagcgtag
     4381 gaatcttacc agatgggact tgcgtcacag atttggatta ccaagaagat tccgcagcac
     4441 ttgtagacat gaatcttgta atgacagaat ctggaaaatt cgtggagatt caaggaactg
     4501 gagaagaagc aacgtttgat ggtgaacagt tgaatgaaat gctctttttt ggaaaaaacg
     4561 cgattgaaga tttgatcaaa gagcaaaaac atgctttgct aacagagttt gctcaaaatg
     4621 atgagcggat agaagaaaca aaaacaatca tcattgcgac aagaaatcca ggaaaagcag
     4681 aagagtttcg aaatatgttc aaagaagcgg gatatcatgt aaaaacatta cttgactatc
     4741 ctgaattgcc agatgtggaa gaaactggca gcacatttga agaaaatgcc cgattaaaag
     4801 cagaaacgat tgcacaattg cttgatcagc cagtcttagc agatgattca ggtttgaaag
     4861 tggatgcttt aggcggtatg ccaggaatct attctgcacg ttttgcaggg gaacaaaaaa
     4921 gtgatgcggg gaataacgcc aaattattat atgaattgac agatgtgccg gatgaaaaaa
     4981 gaacagcaca attccattgt acgttagtat tcgcggcacc caaaaaagac agccttgtag
     5041 tagaagcgga atggcccggt cgagtagccc gtataccatc tggcgaaaat ggctttgggt
     5101 atgatccttt atttatccca gaaggtaaga aacaaacagc agcagaatta tcgagcgaag
     5161 aaaaaaacaa gatcagtcat cgggctcaag caatgaaaaa attgagtgcc gagtggaaac
     5221 aatggctgga aggagagaga tagatgcggt atttagtagt aagtgataat catggagaca
     5281 gagatatcct cgttgactta gtagatcgtt atcgttctga agtggatatc tttttccatt
     5341 gtggtgattc ggaattagaa gcttctgacc cattatggga tacattccaa gtggttcaag
     5401 gtaattgtga ttatggacca ggattcgaac agaaaaaagt gatccaaact ggtcaggata
     5461 cagtattcat gactcacggt catttatcga atgtccgatt tggtttgaca cagttagcaa
     5521 tcgaagctcg agctgcaact gccgatatgg tgttttttgg tcatacccat caaataggct
     5581 gtgagatgga tcagaaaatc ctgtttttga atcctggtag catttctcag cctagaggcc
     5641 cgatccaaat cccatcatat gctcttatcg aaagtttaga tgaccaaatt tctgttcaat
     5701 actataaccg tgcccatcag gcagttgaag agttggcctt ttctttttcg agagagcgct
     5761 gaataaaaaa actctaattt ttaataaaaa taagctttat tttatgaatt ttgcatccgg
     5821 tttcggtaaa atagtagtgt tgaatgaaat agaaatggag gccatcagat gattggacct
     5881 atagtaagag aaatgctgct ggaaaatcag gaaacgtttt tgattcctgc agaaaatgtg
     5941 gcaaatgtta tgtatcatca tccattgtcc catggtttac ttgtgttatc gaaggtcggt
     6001 tattccaaaa ttcctgtatt aggaaaagat gatcactttg taggactagt aagtttatca
     6061 gatgtggtca ataaaatgat tgacttgcaa acaatcagca tggacccgtt agaaggacta
     6121 acggttgctg atgtgacaga aacagatgtt ccgacaattg atgaaaattg ggaactagaa
     6181 gaagtactcc atttgttagt cgattcgcct tttttaccgg ttgtcacaga agatcggatt
     6241 ttcaaaggaa tcattacaag aaaagagctg cttaaagccg taaacttcat ggtgcatgaa
     6301 ttagaacgtc gtaatatatt gtctccgaaa atcgaagaat taaaagaaca aatcgatatc
     6361 gtgagctaaa gaacgagaaa ctaaaagata atcaaaaaat aagtagaaaa aacagcgtac
     6421 cagttttgac tttatcttga aaaagaggct gggacaaaag cgtttaactc caagaaataa
     6481 gaaggaattc acgaaaattg cttttcaaat ttttgtgaat ttcagcttat ttccgaagga
     6541 gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg ggacaaaagt gttctttact
     6601 tttgtcccac gctcttttta gtgagattcg ttgcatcgtt tgtccaaatt attttccgac
     6661 agcaattgga ctgttcggaa tcgtgaaacc agaagctgtc aattctttga cataggagct
     6721 caagaactct tcttttaagg catattgttt tccgttcaat gcgtagcaga ccgtacgaat
     6781 cgcaaaattg ctgtttccaa gatccactaa tccaaagata gaaggttcag tttggagctc
     6841 ttcttggtat ttttcagcta atgtacgatt tactcgatca atgatctcat agattttgtc
     6901 atatccttct tcaggaacaa tccgaatatc taaaagtact tgcatatttg cacgagagag
     6961 attgctgatc gtggtgatgt ttcgattcgg gatgaaatgg acagtaccat ccgttgcttt
     7021 cagctgaagg gtacggatac caacagatac gacggttcct tcaatcgtta agtttgctag
     7081 cttgatgtag tccccaacat ccatctgttg ttccatgata ataaaaaatc cggtaataac
     7141 atcactcatg aacccttggg caccaagacc gattgccaca cctgcaattc cggcaccagc
     7201 caaaagtgat ccgatcggca caccgatagc agataaaatt gcatagataa aaaagaaacc
     7261 aagggtataa tgaaaaatag tatttaaaag actatgtaaa gtctttaagc ggctctcgtt
     7321 tagaatggct tttttagaat actgattaaa agacttatca atcagatatt ttcccacacg
     7381 gtgaagaatc caaaaaagga cgatcaaaaa aatcagatat aaactttttt ggataaaggc
     7441 agccaaaatg gcgtcccaat caatcccgtc ccaaaaccgc tgaaacgcat ttactttgct
     7501 gacagcttga tctgtcaatg tctcgccaga agagtctgtc gtaccggcag ttgctaataa
     7561 tgaaaacata aaatcctctt ttctaaaatt tgattctttc tgtattgtac caagaagttt
     7621 tcaaaaaagg aaatagcaaa aataactata atgaaggaaa aaagctgaaa ataaaatgac
     7681 aaataatatg gataatgata aaatgtggag aaaataacga aggtggtaag tatatgcaaa
     7741 tcgattggaa caatctaggt ttttcttata tcaaaactcc ttggcgtttt attgccaaat
     7801 ggaaagacgg acaatgggaa gaaggagaat tgacagaaga taattacctg acgattcatg
     7861 aaggctctcc agcgcttcat tatggtcagc aatgttttga aggtctaaaa gcttatcgtc
     7921 gaaaagacgg caaggtcaat ctctttcgac ctgaacaaaa tagtcgacgg atgaatcaaa
     7981 gtgctagacg attgctgatg ccaatggtcc ctgaaaagat gttcgttgat gctgtcaagc
     8041 aagtggtaaa agcaaatgaa gattttgtgc cgccctatgg aacaggcgct acattatact
     8101 tgcgcccttt actgattggg gtcggtgaaa acattggtgt ccatccagct ccagaatatt
     8161 tatttgtcgt cttctgtacg ccagtaggag cgtatttcaa aggtggacta aaaccaacga
     8221 attttattgt atctgattac gatcgtgctg ctcctaaggg gactggtgca gcaaaagtcg
     8281 gcggaaatta cgcagcgagt ttacttccag gaacagaagc aaaagaactg gattactcag
     8341 attgtgttta tcttgatcca gccacacata cgaaaatcga agaagtaggt gcagcaaatt
     8401 tctttggaat tacaaaagac ggaaaattca tcactccgca atcgccctct atcttaccaa
     8461 gtatcacgaa atattccttg ttgcagttag ctgaagaacg actaggactg caagcaattg
     8521 aaggagatat tttcattgat gatctagatc aatttgcaga agctggtgca tgtggtacag
     8581 cggctgttat ttctccaatc ggtgggattt atcacaataa caatcttcat gtattttata
     8641 gtgaaacgga agtaggacca gtaacaaaac ggttgtatga tgaattgaca ggtattcaat
     8701 ttggagacat agaagcacca gaaggctgga ttgaagtcgt ggaataattt caaagtaatg
     8761 agttgaaaaa aacgtaaaaa gaaaaaatca ggaagagact ttaaaaagtt ttttcctgat
     8821 ttttatatgt tttactttaa agaatgcaac aaaagtttat gaaagatgga cacctttgtc
     8881 aacgtagata atatcaccga tgattccgct ggaaagtggg ctgattaaga aagcacaagc
     8941 attaccgact tcttcgatcg tcacaccttg ctgatcaggc gtacgagatt cagataactg
     9001 gatcagtttt tgatagtctt taacacctgt gacagctaat gttttgattg ccccagctga
     9061 gattccattg acccgaattc ctttaggcga gaactcagct gccaaataac ggatagcagc
     9121 ttcaagagaa gctttcgcaa tacccatcat gttataattc gggattgcgc gttctgaacc
     9181 tagataagtc atgctaacga tcccgctgtt tggtgcaaga aacggttgtg cgtattttga
     9241 tacagcaatt aaagagtagc tgctgatatc ttgagctaat tgatagccgt ctctggaaat
     9301 atcagacaca tttccggaca attcttcttt gttggcgtaa gcaactgcat ggacaaggcc
     9361 gtggatctcc ccagcatatt caccgatggc attcattgct ttttcgatgc tttcgtcgct
     9421 agaaacatca cattctacag tgaatgcttc ttcgccgacc aatttgacta atgatttttt
     9481 cattcgttca ttttggtaag tatagataat ttctgcccct tgtttcaaca ttgcttcagc
     9541 acaaccccaa gcaatgcttt ttttgttagc gacgcccata atgacgattt tttttccttc
     9601 tagaaatgac ataatgactc tcctttgttt ctgataaaaa tttgatagtc gta
//
LOCUS       DAGCYL010000104         9629 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.105, whole genome shotgun sequence.
ACCESSION   DAGCYL010000104 DAGCYL010000000
VERSION     DAGCYL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.105"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            185..1183
                     /locus_tag="MB216_001907"
     CDS             185..1183
                     /locus_tag="MB216_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4526711.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="MB216_001908"
     CDS             1295..1804
                     /locus_tag="MB216_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBT4526712.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="MB216_001909"
     CDS             complement(1834..2493)
                     /locus_tag="MB216_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBT4526713.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="MB216_001910"
     CDS             complement(2495..3259)
                     /locus_tag="MB216_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBT4526714.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3943)
                     /locus_tag="MB216_001911"
                     /pseudo
     CDS             complement(3267..3943)
                     /locus_tag="MB216_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
     gene            complement(3980..5365)
                     /locus_tag="MB216_001912"
     CDS             complement(3980..5365)
                     /locus_tag="MB216_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBT4526715.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5575..5659)
                     /gene="ffs"
                     /locus_tag="MB216_001913"
     ncRNA           complement(5575..5659)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="MB216_001913"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5682..6197)
                     /gene="tadA"
                     /locus_tag="MB216_001914"
     CDS             complement(5682..6197)
                     /gene="tadA"
                     /locus_tag="MB216_001914"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBT4526716.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6304..7161
                     /locus_tag="MB216_001915"
     CDS             6304..7161
                     /locus_tag="MB216_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4526717.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7242..7577)
                     /locus_tag="MB216_001916"
     CDS             complement(7242..7577)
                     /locus_tag="MB216_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526718.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7644..9014)
                     /locus_tag="MB216_001917"
     CDS             complement(7644..9014)
                     /locus_tag="MB216_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBT4526719.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9092..9628)
                     /locus_tag="MB216_001918"
     CDS             complement(9092..9628)
                     /locus_tag="MB216_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4526720.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2822 a         1985 c         1618 g         3204 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt ttttcttttt tcggcgctga gttgtctttt
     3481 cttttttacc gccgattctc tcgatgacaa aatacgcaca accaaaattg caatattcgt
     3541 aaagatagtc ttccaaggaa tcgattcgct gatctggatg cgcccgtcga ttatccgctt
     3601 ggaaaaatcc ttttaggcgt agttgttcat aaccccaatc tcctacgata taatcatatc
     3661 gggctagtac atcactgtaa cgttctccaa gcttttctgc attaaatcct tcacgataat
     3721 cggacacaag ccgataatgt ctttcaccta tcaaaaaatt actgtcatct aacagtgtca
     3781 ctagctcgcc tttgactttt tctgctttag gctcctcaac gatttcttcc aaagcagctg
     3841 tcagttcttc ggtgactgtt gtttcctgtt cagctgatga ttttttctta gcaatttctg
     3901 ttttatgctc aacgttctgc tgttcatttt gttttgcaga catgcgttca ccacctatct
     3961 tttttattat accttgtttt tatttgattg ggtagtgagc tttcaagtaa tctcctacca
     4021 cacttcggat aaattcaggg aacaaaaatt cattttttcc agcaatctca atcgttggaa
     4081 aaaatggaac aaacatcaaa tgatctacgg tagtaaaagt ataattttta ttcagatcga
     4141 ttggcttgtt cttccaaaag acttgatggt taatagaatc atatgtgatt ccattataga
     4201 ccatctgccc aaaaatctta ccgcgaaatc ccattccctt cataggatag ttacgtaaaa
     4261 agttacgatt tttttctatt tctaaaacta agcggatcag atcatttcca cccaatgtga
     4321 catttaacaa gtgcatcgga tgcggcaacg cagtgtgaag ctgatcctga ttgacccgcc
     4381 cttttggaat ttcagttaaa aacagaccac tgtttaatat tgctgcttcc gtctttcctc
     4441 gttctttcac cgcctcaaag gctacttgga taaacgaatg ctcattgaag atatctagac
     4501 tcagagaaaa tggcaaatct gctacttctt tttcttttaa taattcatgc ccacgttcta
     4561 gataactatt tatttcttgt tcatcttctg taaaagctgt cattgtttcg gtagggatgg
     4621 ctttggctga atgcttaagg atttttttct cttcatcgat ggttaaatgg acttcaccga
     4681 catactgccc gtactttcca gcggctgcta tttggacatc attgattacg tgaccttctc
     4741 tgaataaatg atgggtgtgc gatcctaaaa taacatcaat tgctggtatt tcttttgcaa
     4801 tttggaaatc atcctgaatc ccgagatgac tcatcaaaac taaaacatcc acttgtgaac
     4861 gtaaatcttc gactaattct ggcaaaatat cataaggatt ccgtatgtcc cagccattcg
     4921 gattataagt aagcggaaat ggagcagtaa atgcaattag tccgattttc gtctggtgtt
     4981 tcgttgtaat gattttatat ggtttcgtcc atgccggtgg ctggagcgta tttttatcga
     5041 ataagttatc caggatgata tcaaactcag catgatcgta taaatgattc aactcttctt
     5101 tggaatttcc tacgccttca ttgtttccta tcgtcgctgc atcataacca acttcattca
     5161 tcagcaagat attttcctgt ccattcgttg cttctgacaa tggatgccaa cgatccacaa
     5221 aatcacctag atctactgca atcgttgtcg ttttttcttt cttttcgttt tctcgtttac
     5281 gttgatccaa aaaccttctg atccttggcc aattttctaa atgagaatgc agatcattcg
     5341 tatgtaaaat cactattttt tccatgtacc ctctcgcctc tctttatctt tatcatacca
     5401 taggcagctt tttcttccat tgaaacaagc tctatttcag agtttttcct gccttgaagc
     5461 aaattgtata agtacaatat tcgataaatc agtctggcta aaaaatcagg aacattcatt
     5521 tttacagtac tataaaaata aaaaaaacag acccgaaagt ctgttgtcgt ttcattataa
     5581 tcagacacat ggcggaacct gcttagtgct gcttccttcc agacctgaca cgcttcacaa
     5641 gcccaccatt gtcctagcct ttcggcaaag aatagtatac cttatttttc attcgttgac
     5701 cagtcttttt ttaatttctt ttttcgctgg cggagctttc ggaaaaagtc agtcaaaatc
     5761 atcccacatt cgtcttctaa aatgccacct tctacatatg cttggtggtt aaaccgctcg
     5821 tcctccaaaa gattcatcaa ggtaccggct gttccgcctt ttggatcata cgcgccgaaa
     5881 taaacttctg ctacacgaga caaaatcatc gcgccgctgc acataggaca aggctctaag
     5941 gtaacaaaaa gtgcagcatc ttccaagcgc cagcttccca ctttttcaca ggcttccttg
     6001 attgcaagca tttctgcatg tgtagtagca tcttgcgtgg tttctctaag gttatatcct
     6061 cttccaatca cttttccatc caatacaacc actgctccaa tcggaacttc atgaagcatt
     6121 tctgctttct ttgcttctgc caatgcaagg cgcatccatt tttctttttc tactgcatca
     6181 agatttgtat ttaccaatta cccttccctg cctttgtttt ttcttgtttt actttagcat
     6241 atgataaaaa ggctgtaact aacttttttt tctatggtaa aataaataca agaggtgaac
     6301 aagatgaaaa tggataaatt acttgctctt tacccacaag cacagaaaaa aaactctcca
     6361 gcatcagaag cggatatcct ttcttttgca attgatgatg cttttatctg gatcaaacaa
     6421 gattcattat ctcaacaaga aaccagcctt ttgaaagcgt tattccctgt aatcaacgat
     6481 caaaaaaaac acccatggta tcactatctt tttaaagatg cttcttgtgt tcaagaaaaa
     6541 agctttcgtt tgatccagct tcacgtcgaa tcaaaaggag aattcttaaa aaaagaatgg
     6601 caagatacta ttttggaatt atttcgctat tctgctgatt tcttttttta tacagacaca
     6661 gatgcgctct tagtcgaaca aataaaccgt gattatcttg atgctactga aatcaacggt
     6721 atttttctat ctttagatgc tgattttgat acgacaacgt ctgtattcgt tggttctttc
     6781 cattcacctg gcaccgacct cgtccctttg tttgctgaag aaagaaaaat ttttttaaca
     6841 gagcaaaaca atctttatac tcacagccgt actttttcta tgcaagatgt ggctttacat
     6901 tactatacaa aagatactgt aaaagatagt aagttgatca aaagttacat agaactgatc
     6961 aaacaaaatg aaatggaagt gatcattcaa gaattatgga atgaattagg caatgtcagc
     7021 tctgctgcaa aaacactgtt tcttcatcgt aatactctaa aatatcgaat cgaaaaattt
     7081 caagaacagt ctggtttcaa tctaaaaaaa gccaacgatt tactgttttg ctatctttta
     7141 ttgcttcatg aacataatta aagatagatt aaaggggttg tgacaataat tttgtcacaa
     7201 ccccttattt cgaatgaata gtgttcaaaa gctagtttct cttacatcat tttttcaaca
     7261 aaatgcatta gccggtcttt tacatcttcg gttgaatctt tgattgaatc accataaaca
     7321 gagattttct tttttcctga tttgattttt gctaacattc ccatcaaaga atccaagtca
     7381 ctatctgaaa gaccatctac gatttctagt aatttttcat ttccatcaaa tttgtcatag
     7441 acgaattttt ttactttcgt ccgagtagtc atatgatgga tcttttccat tacttttccc
     7501 gatacaacga ctgctgtcgc tacactggca gcggctgcta cgcttaatcc gattgttact
     7561 tttgttgatg ttttcataag tatcagctcc tttttatcta tctccatcat agcatagaca
     7621 aaaaaataaa cgaactaatc cactcagtga aatctcaatc ctttagggaa aaagttcttt
     7681 actgttcctt ttacttgttt cccaaatccg acaggtatct tatctaatac tagcaaaacc
     7741 catccttgat tcccatcacg ttgaaatgtt tctcctgctg tgtaagattg ccattccttt
     7801 tgagtgatcg gcaagcaagg tatattctct atcttttttg tagcaagcgc taatgcatag
     7861 ctaggttcaa atcgattctt tttgaaatct cctaaatgca aacctgtacg tacgactttt
     7921 aaaccatcga gagaaggggc taactcagga acttcccaaa gatggtcgtt gaacactaaa
     7981 agtcttccag tagcttcata atgaaaatca tttgaaaact ccgtccagag tttttcctgc
     8041 tcttttgtca tttgcacttt actttttttt cgtgtcttct tttctttatg catctgattt
     8101 tgtccatgaa aagtcagctt tgcaacaaaa tgtccttcac cctgatcttt atgaggccat
     8161 atacgtattg ttttttccaa accagctacg cttccccatt cagaacgtcc tgaactaacg
     8221 gattgagtca aaggaatctc ctcaatcgtt actggataat tttctacaag ccagctaatg
     8281 atctcttcat tttcttctgg tgcaaacgta caagtcgaat aaatcagttg tcctttattt
     8341 tttagcattt ttattgctga agacaaaatc tcttgctgac gtttttgaca gtatagcgga
     8401 gattcttctg tccactcttt tattgcattt ggatcttttc ggaacattcc ttctcctgaa
     8461 caaggagcat caaccacgat ccgatcaaaa aagcccgaaa aatgagggac aagttctgcg
     8521 ggtgcatgat ttgtcactat tgcgttcgaa actccccatc gttctatatt ttctgataaa
     8581 atcttcgcac gtttcggaaa gatctcattc gtcactaata accctttccc cttcatttga
     8641 gcagctaatt gggttgattt accaccggga gccgcacaca aatccaaaac tttttcaccg
     8701 ggttttgctg cagcagctgt tccaacgatc attgcacttg gttcttggct atattcatac
     8761 ccagcttgat ggaggaaaga ctttccattg acagtgccaa gaaatccctc attgctgtag
     8821 ggagcgggtt gtaattcttc gctatggtaa gtttggacca tgtccaatcc tgccggtttt
     8881 agtggattcc accgaaatcc ttttttcacg cttccttgtt caagcgcaga aaaaaaatcg
     8941 gatgcttctt cgcctaaaag aagacgatat tttttgataa attgctgtgg taacgttgtc
     9001 gcttcctcct tcattccaat gtttcgttag acattttaca atctttttta cgaaaaataa
     9061 aggtggctga tctacttttt catgttgctg cctaaggtga ctgttccttt aagatatttt
     9121 ccaagtattc atccgtcacc tcgtttttat cccaaacagc gttcatcatt tcaagaaaat
     9181 cttgagaaga atcatctgag cgcaaacttc ccaatgcatt aagccatacc aattcaagcg
     9241 acttttcttc tttgatcttc gtgtgacctt tgacatatga gaaaaatggc cgcttaaata
     9301 gcgaatccat gtctaaatca gattttgcaa tgatttcacg aatactttca tggatagaaa
     9361 aaaaatcttt caataagtag acaatgctta aaacatcatc ggaactgcat tcagctaagt
     9421 agttttcaaa ataaacagtg atttcttcta atatcttctc ttttaacccg ctgattgatt
     9481 tgaaatgtgt ataaatcggg tatgtagaac aaccgcagaa ctctgctaac tctcttgcag
     9541 taattgactt cattccatat tcttccacgt ataccatact tttgaacagc aaatattctt
     9601 tactataatt tgttactctc cccatcatt
//
LOCUS       DAGCYL010000105         9554 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.106, whole genome shotgun sequence.
ACCESSION   DAGCYL010000105 DAGCYL010000000
VERSION     DAGCYL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.106"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            92..1738
                     /locus_tag="MB216_001919"
     CDS             92..1738
                     /locus_tag="MB216_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBT4526721.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIVEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            1729..2811
                     /gene="uxuA"
                     /locus_tag="MB216_001920"
     CDS             1729..2811
                     /gene="uxuA"
                     /locus_tag="MB216_001920"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBT4526722.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(2919..4472)
                     /locus_tag="MB216_001921"
                     /pseudo
     CDS             complement(2919..4472)
                     /locus_tag="MB216_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            4620..5761
                     /locus_tag="MB216_001922"
                     /pseudo
     CDS             4620..5761
                     /locus_tag="MB216_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(5887..6966)
                     /locus_tag="MB216_001923"
     CDS             complement(5887..6966)
                     /locus_tag="MB216_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4526723.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(7069..7509)
                     /locus_tag="MB216_001924"
     CDS             complement(7069..7509)
                     /locus_tag="MB216_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4526724.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(7566..7976)
                     /locus_tag="MB216_001925"
     CDS             complement(7566..7976)
                     /locus_tag="MB216_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBT4526725.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(8156..8590)
                     /locus_tag="MB216_001926"
     CDS             complement(8156..8590)
                     /locus_tag="MB216_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4526726.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(8690..9538)
                     /locus_tag="MB216_001927"
     CDS             complement(8690..9538)
                     /locus_tag="MB216_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4526727.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         3327 a         1714 c         1713 g         2800 t
ORIGIN      
        1 taaatagtaa aattttggtt gacatagcga tttttattgc ataaaattca cttgaataca
       61 aaaactaata tgttagttta ggaggggaaa aatgattgat ttaaacaact ggaggaactt
      121 tatccctgaa ttatctcaaa aagaagtaga aatacctcat tttgaccgtg catcattaag
      181 agaaaatgga caaaaaaatc ctgtctggat ccatttcggt ggcggaaatc tttatcgagg
      241 atttcatgca gaaatcgctc aacgtttagc ggatctaggc gaattgtcca ctggtgtcat
      301 tgtttgtgaa acattcgatg aagaaataat tgaaagggct taccaagatt ttgatgattt
      361 gattcttgaa gtggtcatgc caagcagtgg agagttcgtc aaacgtatat tagctgcgac
      421 agctgacagt ctgtattgtc atccagattg ttctgaacac tttgataaga tgattcgaca
      481 ctttgaaaat cctagtttac aattagttac ttttgctatt actgaaaaag gttatcgatt
      541 gaaagataca aataataaat atataggacc gattgcagtc gatataaaaa atggtccaaa
      601 gaagccatca catacaatga gtattcttgc tgtgctactt tataaacgtt ttttatctgg
      661 agcttggccg attgcacttg tctctacaga taatttttca caaaacggta gtcaattacg
      721 tacttctttg ctaacgatcg caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt
      781 tatccaatac ttatcgaatg aagaaattgt tgcgtttcca tggacgatga ttgatcgtat
      841 caccccgaat ccttccttgt cagttagcaa aatgttggca aaagagggct tttcaaatat
      901 gtcgattttc caaaccgcaa aaggaaataa tattgctcca tttgtcaata cagaggacat
      961 ccattattta atcatggaag atcattttcc caatggacgt ccgaaattcg aaaaagctgg
     1021 ggtattcttg actgatcgtg caacggtaga taaagcagat acgatgaagg tgacgacttg
     1081 ccttaatcct ttgcatacgt ctttagctat tatgggttgt ttactggggt ataggagcat
     1141 agttgaggag atgaaagatc cagatttatc gctgctagtt tctgaaatcg ggtacaatga
     1201 aggcctccca gttgtggagt ctccaggtat cattgatcca aagatctttc tggaagaagt
     1261 aattactgtg cgcttttcta atccaaatat tcctaatatg cctcagcgaa tcgccacgga
     1321 tacctctcaa aaggtggcga ttcgttatgg tgagacgatt aaaaaatata tggaaataga
     1381 agttaaagac ccgcagacac ttgtattcat accattgact ctcgctgctt ggcttcgata
     1441 ccttattggc attgatgatc aaggaaaacc attcatcccg agtccagacc cactattgac
     1501 agaccttcag cataagcttt ccgatattca actgggtacg caagatacat cacatattca
     1561 tgagaaaatg gctcctattt tgtcaaataa agaaattttt ggtgtagatc tttaccaagc
     1621 agagcttggc agaaaaatag aagaaatgtt catcaagatg gtagcaggac caaatgccgt
     1681 acgaaaaaca ttacaggatt acttacaaga acatggagga aatttatcat ggaaatgact
     1741 tttagatggt acggatcaca ggaagaaaag attaccttag atgaaatcag acagattcct
     1801 gcggtcaaag ggattgtagg cacattgatg gatataccag taggagaggt atggcctagg
     1861 gagaggatca aagaactaaa agaagaaatt gagtctcgtg ggttaacact aaaagtaatt
     1921 gaaagcgtca atgtgcatga tgccattaaa attggattac cttcccgtga gcagtatatc
     1981 gaaaactata agcagacaat ccgaaacttg gcagaatatg gaatagaagt catctgctat
     2041 aattttatgc ctgtatttga ctgggtgaaa tctgaccttg actaccgctt agaagacggc
     2101 tcatccacgt tagcttttat atctgatgat attccagatg atccaagaga aatcgtcgcg
     2161 cgcatcgaac attccagcaa tgaatttgga ttgccgggat gggaacctga gcgcttagct
     2221 cctatcaaat ccttgcttga agcctatgca tcagtagacg aagaaaaact tcgagaaaat
     2281 tttgcctatt ttctccaatc aatcatccca acatgtgaag aggtaggggt caaaatggct
     2341 gtacatccag atgatccgcc atactcgatt tttggattag ctcgaatcgt aaagaatcga
     2401 gaagatttag attggttgtg tcgcatagta gacagccctt ataacggaat cactctttgt
     2461 acagggagta ttgcagaaga tccagaaaat gatgtatatg ggattttagc agaattttgc
     2521 caaagagatc gaatcccatt tgcacatgtt cgaaatatta aatttttagc taatcgagat
     2581 ttctatgagg caccacataa atccgaatac ggttcattag acatgtaccg catactaaaa
     2641 acaatgtatg acaacggatt taacggatac atccgtcctg atcatggaag aatgatctgg
     2701 aatgagacag gaagaccagg atacggctta tatgatcgag cattaggtgc tgtttatcta
     2761 aatggattat gggaagcaat cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa
     2821 ctgtaatttt attatgtaaa taagaaggat gttcaaattc ttgatacata aatccggagg
     2881 ttgttaataa gcattcggat ttatgtatct tatttggtct aggaagtaac ttgattttct
     2941 tttttcgatt gatccatgag tgcttcttct tttaattcaa agatatattc tgagatcctc
     3001 ataatcactt ttttccagtc ttctggtgtg atgattcctt ttaaaatata gatttttttg
     3061 atatgagaag gcaattgttt caaagggcag tcgctgataa cgatgttgca gttctccata
     3121 aaatcatttt gtattttgat cgattcattt gaaaaaattt gttctatctt aacgatcaga
     3181 tctttactga tataaaaatc acgcgtatag ttcacaccaa tcacaattcg cggctgtttt
     3241 ttgtagcgat catacaaatg tactaggtct tcaaccatcc attgcacatt ttccttttct
     3301 aaaataactg gtaacaagtg ttgattttgt tctctgaaat ccatataaaa ttgttcaatg
     3361 gcggtttgga cttctttcaa tacgctatgt tcttgataaa gcatgaaagt aggaaggggc
     3421 gtatttggtt gattgaagtt aagaaatcca ttataaagtt tatttcgtgc gaatgccaat
     3481 gagtaaagaa tcttttcttt ttcaggtata aacaaatcaa atacagtaca aagggactcc
     3541 accatttctc ttgaataaag aacatgagga acgtttgctt tttttaaaag tttgtattgc
     3601 tccaagcttg tttcttccga ttcgattttc gtgatagaaa gtcgagctaa aatattcact
     3661 aagatgcgtt gttcgcgaca aattggcaga ttaggtcgca aaatatctac agagagatca
     3721 ataaaaaagt caatatattc atactctgtg aattcttttt gtacgtcttt cacagaagtt
     3781 gcaggaaaat tctctttaca tatttttaat agaaaatata atttatctat cataccgcta
     3841 tttagtcggt caagtacttc ttttttaaaa aaagtattca tcataaaagc cactacttgc
     3901 ggatcttcat cataaaattc tgtagcagta cttgaaaaga tgttccaata aatatctaat
     3961 gctgcatatt gaatttgtac ctcttttcca gaaaaagact ttctgccatt attagaaaag
     4021 gagatagaca aaccgtacta tgctaaaaat gcattaatct ttttcatttt cgtatacaaa
     4081 taagaggcac tatagtttgt ttgctgactg aaactgataa tcgaatttgt ctgacagctg
     4141 aacaaagaag tcaagatctg ataactgact gattcgttgc agtatcttcc aaatagacga
     4201 taatagcagt aagccggatc aattccttca aaattcaact ttaaaaaagg tttttgctta
     4261 tcaatatgca gttctgttag attgagacgc tcaaagtctt gttctaattc tttgatgtat
     4321 agtaaaaaat ttgattcaga catatggaaa tgatctgcta aatattgtgt agtaataaag
     4381 aaacgatgtt cagataggaa aagaaaaata tctaattttg tattgctttt tttcttcaag
     4441 ataaacgttt taatcgcttc ttgagtttgc atgaaagagg ctccttttta cttaaattgt
     4501 taaaacttga aaatattact gtttttttca taaatttaga caaaaaaaca gtaaacatat
     4561 aggtagaatc tctactatct tatttcttgc atacaaaaaa atcaaggagt gagagagtta
     4621 tgagatattc aaccatctta atatcaacat ttgcagcctt aagcttatcg cttactataa
     4681 atcagacagt ccacacagag gagacagaaa cagccaaatg gacagaaacc aagtgggcca
     4741 acgtcaatcg aattaccttt caggatgaat tttcatcctt caattcagga gtcgcaaaat
     4801 taagaaaagg acaaacagcg accatcaaag caaaaattga ctattcggga gacttagcac
     4861 ctatactaca atcagctgtt acatttgaga atgttgatcc cactttgtcg attggctatg
     4921 acgagaacac tttgactatc caacacaagc aagcgatatt taccttcacc gttacattaa
     4981 atcaggactt gttaaaacca gcgcttttta ctgtcaaggt atcagatgga ctaccgaacg
     5041 atcatcattt aacgccgtat agtcagcggc agacaattga gcaagattca gccatcgact
     5101 caggaggaga tcttgttgaa gaaccaacag ataaaccaga aaatgaaaat aagccagaag
     5161 taccgcctac agaaaatcca gacggagaac agaaaccgga aatagaacct ggagaagaac
     5221 ccgatacaga aactcaacca gagccagaca ataaatcaaa acctgaaata acccctggag
     5281 aaaaaccaga tgtaactcca gaaccagata cggattctgg aaaccaaaca gtaccggaaa
     5341 caaatccaga cacagataat gaaactgaaa atccagaaaa accagaggta gatccagaag
     5401 aaaaaccaga tgtgactcca gaaccagata cggacgcaag agaccaagga ataccagaaa
     5461 aaataaacaa aaaaacaatt caagaggatg gaaaaaaaga gagtaaaaaa tcaaatctag
     5521 caattctaaa aataaatgaa gagcagctga ataaaaaatc acgaatattt gatagcgccc
     5581 aatcagctga aaccttaaaa agtagcaagg atacaacctt tgctgtcctg aaacgaaaaa
     5641 taaacagcta ccaaaatccg gagagtcaca aaacaaagtt atactgtgga gcggaatcat
     5701 tctattaagt atagcaacaa tgctttctgc aaaaagattt aaacaaaaca gatctctcta
     5761 acatttgaat aatgtctgtg aacgcatttt tcaaataaaa agaaatatcc agaaaatgac
     5821 cccaaaatac cagattgaaa tctgatattt ggggtcgttc atactttatc tttcttcata
     5881 tttattttaa gggatatctg tttcttttgg aagatggttc gggatctttt tcactttcaa
     5941 tacatctaaa aagaaggtaa agatcggaat gccgacaatc aagccccaaa cgccaaagaa
     6001 ccgttcgcta acaagtaaga ccacaaatgt ataaaaaatc ggcagttctg ttttgctgga
     6061 catcaacttc ggattcagca cataagactc aattagatgg acaatcaaaa tcgtaatcag
     6121 gatataaaca acatctcgga tgcctccttg ggaataagct atgaaagata gaggaataca
     6181 agaaatgata acacctgcta ctgggatcag acttaaaatg aaaatcatga tcgctaaact
     6241 cagcagctga tgaaagccaa atacagccaa ggcgattgta gtgagcaacg tatttaaaag
     6301 agcgacaaca aattgagctt ctaataccaa accaaatgta ttgacaaact tatcggcaaa
     6361 atagtaaata tcttgaaaga accaagaaaa ttcacttttt aaaaacaatc ttgagaatac
     6421 aattgttttt ttcttttcaa tcataaaaaa gaaacttaag atgaatgaca tagcaaacgc
     6481 cactgccatt tttcctatat cctgtaaata gccaagcgca atcgaagcgc cattttgaag
     6541 ctgttcaaaa aaatcgtttc gctcaatata agtatggata tattgtaaaa caggatctgc
     6601 atcttttggc tgatgctcgt aaaaattaat aacggactgg atcatgtcaa atgtttgatg
     6661 aataagcatc gggatatact ttgtgaatgc cagatagata aggtaaacga tcagtgtata
     6721 aatcatgata gctaaaacac ctgtagggat tcggacaaat cgctgaatga tttgaacaag
     6781 atgaatcatc aaataagtaa agataaatgt gagcaaaatg gttgtaatca tacttctaac
     6841 taagaaaaga acaaaaacga caaataataa aacagtgaga cggcgtattt tttcattcgc
     6901 caaaaaacgc tgataaacgg tcaaatcgac aacctccaaa taacaaaaaa taaacacgtt
     6961 attcattata tgatggaata tgaaaataag aaagagcaaa aagaaaaacc gctaaatgac
     7021 gttatcttat gagttggttt tcctcataaa atcgttcatc tagcagtttc agcccttttt
     7081 ctggtcttga tgaaaattat ctagcatttt tcgggtcaga tgttgcagtg tatccaattc
     7141 ttcctgttca agtggaagat aagacttgat cttttgtgga ataccttctg cttgtttttg
     7201 caacgcagca ccttgagcag tcagcgtaac atttacgatt ctttcatcac ccgtttcacg
     7261 ctgacgatta atataatttt tttctgctaa cttttttagt aagggagtca gcgtaccaga
     7321 atccagacac aaatcatttc ctagctcttt cactgaagtc tttttctttt cccataaaac
     7381 taacatcaca agatactggg tataggtaag attcagcggc ttgaggaggg gagtgtagtg
     7441 tctaatgatt tctttagagg cggcatataa aggaaaacat acttgattaa ctaaatatag
     7501 atcttccatt tttccacctc cgctgtttta ctatagtgta tcatagcgga tttacacaaa
     7561 aaagtctatt cactaactgc tttcagagta acttctatat ttccagctgt agcttttgaa
     7621 tacggacaca ctttatgcgc tgcctctaat aattcttgcg tttcttccag agaaagacct
     7681 tcgatagatc cttcaatctc tacacctaag acaaagctcg tttcactttc tgaataaagg
     7741 gaaacttgtg cactgacagt agaagcccca gagatatttt tttgctgttt gatcaattct
     7801 aatgcactgt taaaacatgc actgaaacca gctgcaaaca attgttcagg gttggttgca
     7861 ttttcgacgc gtttaccagg agctgcaata ttcaattcga atgtacgatc gggattatgc
     7921 acttctccag agcgtccgcc tgtgttgatc attttcgtag aatacatttt tttcataaag
     7981 ttaccacctt taattatatt ttatacaatt gaattttaca caatataatt gtggaaagca
     8041 aagaaatgga ttggcataag ataaataaga gaaagcacat gaaacagacg cgctttctcg
     8101 aatagcaaac atatacaaaa aatatcagag acaaaaaata atctcatcat aaacattaca
     8161 tatatttctc aaaaagttgt tttctttctt caaaaattac ttcacgatgg acctgatgaa
     8221 gcatccataa ataaccaaaa ggatctctga acattgagtt agtcacacca aagtccatca
     8281 tttctgtcat ttcttgaatc tctacacagc catttcgtaa tgctttatta tgtgtgtcct
     8341 cgatatcaga gacgatgata ttgaaccaga tcgtatttgg atgttcagca gtaggagcag
     8401 tcaaaccaag agacgtgttt tcatccagca tatggaatct cgtaccatac aaggtaaaaa
     8461 tgacttcatt attgcctcta gggtaatctg ttgcctcaat cagttgtatt tcatcaaaga
     8521 ttgtttgata cagttctagt gctttcaaac tatcatttaa tacaaaatcg atttccgttc
     8581 ctttatgcat aaagatctcc tctccttttc agtcgaacag ttactagatt cttttattat
     8641 accagctaaa gaaaacgcat tcaaaaaaat agcggattta cttaccaact cattcgattg
     8701 gatgataagt aaatccgcgt cccattactt ctgctacgct gctgacaatg acaaaggcac
     8761 ttggatcgat ttcttctatc aatggtttca ttcgggaaaa ttcatgatca gtcacaacga
     8821 tcaataaaac ctcttttgac tttcgattat acccgccttt tgcctctagc agggtaaggc
     8881 cacgtccaat ctcagcactt aagcgaaggc ggatgtcttc caaataatgt tcactcatga
     8941 ccataacaga tttctttcga ttgatacctg tttcgatata agtcatgaca attgaagtga
     9001 ttgcaataga taaaatagca taaagaaact cttctattcc aaaaataaac agattaagag
     9061 aaacaacgaa tgcgtctgta aaaaataagc caatcgaagt attcaagtgg aaatattttt
     9121 taaaaatgag gggagggatc gtcgtaccgc cgctagaaga gttgttttta tataaaatcg
     9181 caactcctaa agcaaaaatc gcacttccaa aaatgacaga caacaaccga tctgaagtaa
     9241 tcattgtttc tggaactatt gcaatgatta aaggaaaaac caagcttccg aaaagtactt
     9301 taaaaaaggg ttttttacct aaaaatagaa gagctagtac caacataacg atattcaaca
     9361 ctaaaataac tatagctcgg tcaaagccta aagcagcttc cagtaatatt cctagaccgc
     9421 tgacacctcc agcagcaata tgatgaggcc ctaaaaacat attgatagcg actcctaata
     9481 tgaagagggc aaaaataatc ttaatgtatg tccatataaa ttgtctcgtt tctttcattt
     9541 attcacttcc ttaa
//
LOCUS       DAGCYL010000106         9545 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.107, whole genome shotgun sequence.
ACCESSION   DAGCYL010000106 DAGCYL010000000
VERSION     DAGCYL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.107"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            147..1016
                     /locus_tag="MB216_001928"
     CDS             147..1016
                     /locus_tag="MB216_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBT4526728.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="MB216_001929"
     CDS             1225..2250
                     /locus_tag="MB216_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBT4526729.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="MB216_001930"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="MB216_001930"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBT4526730.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="MB216_001931"
     CDS             3333..5036
                     /locus_tag="MB216_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4526731.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="MB216_001932"
     CDS             5038..7071
                     /locus_tag="MB216_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBT4526732.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="MB216_001933"
     CDS             7082..7522
                     /locus_tag="MB216_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4526733.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="MB216_001934"
     CDS             complement(7722..8849)
                     /locus_tag="MB216_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4526734.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="MB216_001935"
     CDS             complement(8852..9529)
                     /locus_tag="MB216_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4526735.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
BASE COUNT         3239 a         1653 c         1953 g         2700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caata
//
LOCUS       DAGCYL010000107         9355 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.108, whole genome shotgun sequence.
ACCESSION   DAGCYL010000107 DAGCYL010000000
VERSION     DAGCYL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.108"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(189..1556)
                     /gene="nhaC"
                     /locus_tag="MB216_001936"
     CDS             complement(189..1556)
                     /gene="nhaC"
                     /locus_tag="MB216_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBT4526736.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1665..3086)
                     /locus_tag="MB216_001937"
     CDS             complement(1665..3086)
                     /locus_tag="MB216_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4526737.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3329..5206)
                     /gene="tdc"
                     /locus_tag="MB216_001938"
     CDS             complement(3329..5206)
                     /gene="tdc"
                     /locus_tag="MB216_001938"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBT4526738.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5497..6753)
                     /locus_tag="MB216_001939"
     CDS             complement(5497..6753)
                     /locus_tag="MB216_001939"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBT4526739.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(6865..7070)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7333..8634
                     /locus_tag="MB216_001940"
     CDS             7333..8634
                     /locus_tag="MB216_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBT4526740.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8703..9302)
                     /locus_tag="MB216_001941"
     CDS             complement(8703..9302)
                     /locus_tag="MB216_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4526741.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2833 a         1939 c         1665 g         2918 t
ORIGIN      
        1 aaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
       61 cggcggaagc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttaaaaagc
      121 aattttcttg agtttcttct tatttcttgg agttaaacgc ttctgtccca gcctcttttt
      181 tccttccttc aattttttac acgtcgacga ttcaataaaa aaccgcctaa tattgtaaag
      241 acaggtgcaa gaatcgagaa aaaggcgaat ggcaagtaat gcaacgcgct aactttcatc
      301 gttcccataa tgaatgtgcc gctgactccc cacggtacga gagaattgac ggtcgcacct
      361 gaatccgcta gtgttcgtgt caaataggtt tttgataagc caacgtcatc aaaagaacgc
      421 ttgaatgttt ctcctggaag aatgatggac agatactgtt ctccaacgat cagattgatt
      481 ccgatacagc tcaaggcagt aaacccgact aaacgagaag gtcggtccat cttttctttt
      541 aattcttgaa cgatggtatc aacgatcaga tatttgatca gtaacccacc taatgccaat
      601 gcaagtaaaa tcaaactagc agaacctaac atactcatga tcccgcctcg agaaagcagt
      661 tcatctagct ttggatcagg tgtatttgcg acataaccat tcatcaagat gtctgctgct
      721 tttccgaccg tcaaactttt ctcatggata aaagaaagaa cgagagcagc tgtcgatccc
      781 gctagtaatg tagggatagc cggaattttt ttgattgccc ccccaaataa aataactagt
      841 ggaatcaatg tccaaggaga gatccaaaag ctcgctttca tgttttggat catttcattg
      901 atactagcta gatcggctga atgactgtta tgtcctaata tcgtataaaa aatcaacgaa
      961 aggacgaatg ctgggaaatc cgtataacgc ataccagcga tatgttcgaa tagattaact
     1021 cctccaatac ccgctgccaa gttggtcgta tctgataaag gcgagcagtt attccctaaa
     1081 aaggcaccgg aaacgactgc gcctgtagtc attgcatcat tgaatccaag taaatgaccg
     1141 atccccaaaa aagcaattcc aatcgtcgaa atcgttgtaa atgaactgcc gacagtcact
     1201 ccgacaattg cacaaacaag aaatgctgtc ggtaaaaaga aacgcacaga aacaagttta
     1261 aatccataaa ccataatcgt agggatggtt ccagataaaa tccatgaagc aactaagaca
     1321 ccaatcaaaa gaaaaatcag gattggaatg atccctggag tgattccttt gatgatcccg
     1381 tcatggatct catcccatgt gaacttttta aatctgccat aaaacaacaa acacatgaaa
     1441 gctgcaagga tcgggatatg tggcgagagc tggaatccga tgatcagtac tcctaggagt
     1501 gccaacaaca cgatcaatac agcgatactt tcttttaaag aaatattttt attcatcgtc
     1561 gatctcctat ttcaaatgaa aaggccctgc catgaaaaaa ctattcactt ggcaggacct
     1621 cctactaaat cagcgacaaa acgtcggctg aaagacttat ttttttattt ttcttctaaa
     1681 cttttttcta cctcaatccc ccaagaatct ttcttgaagt gattgatgat caatggaata
     1741 acgaacatga ccactgtgat agctgctact aataatgcat cactcaatgt cgctgtacct
     1801 aatgtagcaa atacgacaac gatgatagag aagatcagca tgcagctgac tacccaagct
     1861 aatccgtttc cgtttttacc aattcggtaa ggacgttcga attctggatg tttcttacgc
     1921 attttaagca atgaaacagc aatcaaaatg tacacgatac aataaataac agttgtagca
     1981 tttgtaagtg tcaagaatac agcacttacg tcatccatta caccataaag caaagcaaat
     2041 aatgaaataa cgattgattg tgttagaacg acatttctag atacgccgta tttgttttct
     2101 ttatggaatc cgaattttgg tggaaggaat ccttcacgag ctacttggat gattgttttt
     2161 gatggtccag taacccaagc tgataattgt aaaagcacac caataaatac catgaagctg
     2221 aagatatttc cgatcacttc tggtaagcct aggatttgac agtcaatcaa gataggctgc
     2281 gtgatatttg ctaattccat ttttccatct ggtacgacat ttgctacaag catcgcattg
     2341 atgacattca ataaaactaa tccgatcaaa gcgataaata cgccttttgt ataatttttt
     2401 gttgcgtctt tcaaacgagg catgtaaaca gaactcattt cgatcccaac aaaaataaat
     2461 gtgataccag ctaagtattt caatgaatca atatgttcca agtcagggaa agcttttgac
     2521 catgaaaagt cacctagata gccgtttgag actagtccga ctttgaaggc tgccaaaaca
     2581 cctaaaacga acatgatgac tactgggata tataccccta gccatacacc gatgttcccg
     2641 ccgacttttg ccatatcgaa tttcaagttc aagatcgtga tgatccagta gataactaaa
     2701 atacaaccta aaacgaacca gtgattattg ccaagttcta cattaccaaa tgtgtttcct
     2761 aacaatggtc ctaaagtaga agcgaccatt accatacctg ggaacatttg cacccataat
     2821 agccatgcaa cgacaaatcc ccatttgctg cccagtgctg tttttaccca taactgagga
     2881 ccgccttctt gaggaagcat cgtggaaagc tcaccggaaa ccattgatat cggaccggca
     2941 aaaaacaaaa ctgcaaaaat tgaataaaaa atcaatgtcc aaccgactgc ggctaacgta
     3001 ggaatactcc gaaccgttgc acacagtgcc attgtcagac cgataaaggt cccaagaccc
     3061 attttactta cttcagattt actcaccgtt gacactctcc ttgatttttt ggataaaata
     3121 acggtgtctt tgaagtagtt tcagcaataa acgaaacaat tcagcgaata tttgagggga
     3181 ttctttgagt ttattgcttg aagggtttct tcaaagacaa cctcaaaaca aagggatcga
     3241 agagcttagg tctccccttg ctcttcagat ccaaacatgc gtcagaaaca gttagtattc
     3301 aagctgtttt ctaacatgat taccttgctt attttgcttc gcttgccaat aattggtctg
     3361 cgtagatttt ttccaatttt tcttgaagag ctgctttgat tttttcagca tattcttcaa
     3421 agtgttcttc tttgttcatg tatggtgtca ttacgctggc acgcaataca gttacttttc
     3481 cggcacggtt ccattcttca tctgagaatc ctagttggtt cacaaattgt aatgggctgt
     3541 tgccatagtc tgggatagca aagtcagtat gtgaagttaa gaactcatta ccgtaaatgc
     3601 ttcctttcac gtatgaagaa taatcatata cgtcatggtt caatttattc attgcaacta
     3661 agtcatcgtt gcctttttct ttgaatacat agtctaccat attgaaatct ggataagtta
     3721 atggatgaac ttcgatttct ttgtctccta ctttaaatga caagtcattc aagaagttgt
     3781 agaaacgatg tgcaccttcg attgatgcac ccattaattt accatatcct gtgacattca
     3841 aaggtaagac atggtgtgct gcccaaacgc tggctgctgt tgcccctgct tttgaacctt
     3901 ccaagatata agccccaagt aatgctggga tatctgcgcc tttttcaaat acatatgtcg
     3961 caaagtaaga gataacgtct ctcatgcgga tatctttgat gacgatacca ccggcagaat
     4021 atggaacata gcccatttta tgtgggtcaa tcgttacaga ttctgcttct tcgattgctt
     4081 tatacgcact gtgtacttct tctaagatat aatctttgtt ttctgtaaag acattgtatt
     4141 tgtaatgaac atcttttaaa tcttcaaatg gaatgaagtt gttgtcttcg tctaagaaaa
     4201 tcgcacggcc gtatccgcca taagcagcat ctacgtgtaa gtagaagtaa ataccgtctt
     4261 tttctaatac gcgacgcaat gcaacgattt tatcgatacc gtcgattgct ccttcttctg
     4321 ttgatcctac aacaccgact acacctagga ttggtgtttt ttcagctgct agaccacgaa
     4381 cgattttttc aagttcgttg atgtccatgc gatagttgtg gtcaactggc actggaatca
     4441 cttggtctaa accgataccg atgatgtcgg cagcttttag ccatgaatag tgtttagttt
     4501 gtggaaccaa ccattttcct aatttttcta aatgttttcc gctacgtgct gagtgagctt
     4561 tgatttcgtc gattttttct gggacgctgt ctaacagatt catgatttct tctgttgaca
     4621 agttcattaa ttcccaatcg ctttttccag caactagttc aggtgtcact tctttcattg
     4681 caagcggcaa tgatttgatg ttgcgggcat accaaagtcc ttctaagttt gctaaagaac
     4741 catcagcaac gatgtggccc cagccatctt tatagctcat taattttgca aattccattc
     4801 ctacttcttc ttccatttgg cttgttgcag gagaagattc ataggcaacg ttattaccat
     4861 tccatagcat agcaaaattg tacgctagta aagatggcat caatgtttca gagttcatat
     4921 gaccccagta acgtccggca ttatgccaag gaacggaatg ggtacgcatc cgcgctgaaa
     4981 tttctgatag aacatccttt gttttattca cagtgtgttc aaaactagca ctgctttttt
     5041 cttctggcgt aataataggc atgtcttgag gcatgtagtt ttgacgccag cctaaatgtt
     5101 catccaccaa ttcattcaac aaagctttat agatttggcc gttctcagct ttgtccccaa
     5161 tgaatagggc atttaggttt agatctttcg acaatgattc actcatagtg aaaccctcct
     5221 aaaaaaatat ttaaattttg tcgagcccta cattacacct gttcttctat tctgtaaact
     5281 aagttttggc ctgattttta atcatatttt aatatgtatc aattatccga attgttcaca
     5341 cttttgaaat aattaaaaaa aacgagggaa tttacaaatg taaactccct cgcaaagatt
     5401 gtattaacga caaccagcgt tctattttag gatgggtttc aaaattggaa tgttgtaata
     5461 gtatttccct ttttcaaggt aaaaacttac taaattctaa tcaattactt tggccaaaaa
     5521 gtaatttttc ttaccttttc gaataaccac aaacttacca tcgaattcat cggatggatt
     5581 tatgacaaaa tttaaatcgg taacgcggtc accgttaata cttatagctc cgtttgaaac
     5641 atcttcacgt gcttgacgtt tagatggttc aatttttgtg gaaacaagca gttccacgat
     5701 attttcaggt gtgcttgaaa tttcaacgtt cggcatttta ccgaatcctt gcgcgatttc
     5761 ttccgcattt agttctttaa tatttccaga gaacaatgct tgcgttattt tttgcgcttc
     5821 tttcaagtct tcttcactat gcacaaatct tgtaacttct tctgccaaac ggcgttgtgc
     5881 ttcacgtttc tcaggttctg tctctacttt tttcgctaag tcttcgattt cttcttgaga
     5941 caagaaagta aagaatttca agtatttcac tacatcgcga tcatcttgat tcaaccagaa
     6001 ttggtagaac tcaaatggtg aagttttctt cggatcaagc caaacggcac cgcctgcagt
     6061 ttttccgaat ttcgttccgt cagccttcag catcaatggg atcgttaaac cgaacgcttc
     6121 tgcttctggt ccttctaatt ttcggattag gtctaatcca gctgtgatat ttccccattg
     6181 gtctgctcca ccgatttgca attgcacatc gtatgtttta tgcagatgca aaaagtcgat
     6241 agattgcagg atttggtaag taaactctgt aaatgagatt cctacttcca aacggctggc
     6301 aacgatatct tttgctaaca tcgtgttgat gttgaagttt ttaccgtagt ctctcaaaaa
     6361 ttcaagtaaa gagatttttg acaaccagtc gtagttgttc acaaatgtga tatttgcatc
     6421 tttaccaaac aattttctca tttgattgga aagtgcgtcc acgttatgtt gcacttgttc
     6481 catcgtttgc aacacacgtt ctgttttgcg gccgcttggg tcgccgatcg ttcctgttcc
     6541 gccgccaatc agaatatatg gatgatggcc tgccaattgg aatcttttca tcatcataaa
     6601 tggaatcaaa tgtccgatat gcatcgaatc accagttgga tcaacgccgc aataaagtga
     6661 aatgctcttt tcttcaacga gctcgcgaag tctttcttcg tttgtttgct gattaatggc
     6721 gtcgcgccaa gttaattcat caataatatt cattttactg acctccgtgt agttttttta
     6781 tgaaagcagg aacctgctag catgtgtaat taattatttc ttcaggatag gataggaaat
     6841 aaaaaaagtc ccttggcata taagccaagg gacgaaatct cgcggtacca cccaatttgt
     6901 atgtctagat atccaacata cctctcgaac atttaacgct gtcgcgtggc aatcactgcc
     6961 aaagactcct gaaatgtaat tcgctttaaa gcatgtactg gtttgcagcg accaccagct
     7021 ttctgaaaca ttggctttaa caactacttc gtttcgatct tcgtcttgtt attattaata
     7081 tgtgtctatc atagagcgca actttttctt tgtcaacaga aaaaggatat tttttttaaa
     7141 ttcctcattt tttctttttt gaatccgctc taattccgaa cattttattt tcaattattg
     7201 ttaaaagaaa taactttttc tgtatttttt ctgcaattta tcgagaagtc actttacttt
     7261 gcctcttatt ctttcttcgt tacaataagg aatgggacaa gtaaaaaaat gaaaaagcaa
     7321 gggacgaggt atatggaaaa aaagcaaaaa attattttag gagccacttc tgctttaact
     7381 atcgttattt taattctaag tttgtttttt gtcatccgat catctacaaa aaaagaacca
     7441 atcattcctg aaaaaaaaat aacagaaaaa gcaaaagaac cgacagatga taagcaacaa
     7501 ctagttactc aagcaaaaaa tctggctcaa agctacgatt atgaccaagc gattgcgtta
     7561 ttggaaaaag atagtaagaa cggatctact gaaacacaac agcttcttca aacactaaaa
     7621 aaggagaaag aacaactgat cgactggtct gatcctactc agatctctca tgtctttttc
     7681 cacagcttga tcgtggaccc gcaaaaagcc ttccactctc aacaggcaca aggatacaaa
     7741 gattatatgg tgactgttga agaattcaac cgttcgatcg aacaacttta tcaaaatgat
     7801 tttgttttag ttaatttgaa tgatttgatc caaaaagacg aacaaggaaa tctttccttt
     7861 aacgggcttg cgctacctga aggaaaaaaa ccattggttc tttcacaaga tgatgtcagc
     7921 tattatgaat atatggataa ttccggtttt cctagccgat tggtcgtatc aaataacaat
     7981 gaaatcaaaa atgtctataa agagaaagat aaagaagaaa tcggtgactt tgacatggtt
     8041 cctctgatcg atgcctttgt caagaaacat cctgattttt cttatcgagg cgcaaaaggt
     8101 accttggccc tgacaggtta caacggagtt ttaggttatc gaacatcaaa atctcaatat
     8161 ggagacaatg aaaaaacaaa aaaagagatt aaagaagcta aaaaagtggc ggaacagttg
     8221 aagaaagacg gctggagctt tgcttcccat acatggggtc acttgaatat gactcaagct
     8281 tcactagaag atgtaaaaaa agacaatgaa ttgtggcagc aagaagttgc acctatccta
     8341 ggaaaaacaa atattctgat ttatccattt ggtgcagaca tcagcgactg gcagccttat
     8401 tcagcagaaa atcaaaaatt tgcttacttg aagagtcaag gtttcgatat tttctgtaat
     8461 gtcgatgctt ctacaccagc ttggggacaa ttaggtacgg atttctatcg aaatgctcgg
     8521 atcaatatcg atggcattcg ttttcaatct gatttagacg gaaaaaatcc tattcttgat
     8581 ccattcatca atgtccatga tgtctatgac aaagaagcac gcgaatctgc ttgattttac
     8641 gtcatttatg aaccaaaaaa gccgaactga cattttatcg tcagtttcgg cttttttatg
     8701 ctttatttct tcttcggtgc gattcccttg atcaggactt ctgtccattc tgctgtatat
     8761 ggaagcagtt ttgcgtatat ctccggctta gaaattcctt ctaccattgc ttgaaaatag
     8821 aggatcaaga actcatccga atatttcaaa tccacttttc cttctttccg ccctcgatgg
     8881 aaaagatcaa gcatgatact gaaactttct tgcgtatacg catccatcgc gtcagaaaat
     8941 tctttttttt ctttttttgt atagtatgtc atcaaatcga tataaaattg tttgttcact
     9001 cgattgagat aacggatttt gttttcactc atggcaatca gtgtttcttc aaatggaaga
     9061 ttttccgcaa tcaacgcttt cgcttcttgc cctaaatcat tcataaatgc aaaaaatact
     9121 tcataaatca gcccttcctt gctgccaaaa tatttaaaaa tcgtcgcttt acttacctga
     9181 gcgttctcag ctaccttatc cattgtcact ccagcggttc ctatgtctgt gttcatcaag
     9241 tcaaaagctg cttgtaaaat ctctttcttt ttctcttgtg ctctcttttc aaaaccgttc
     9301 atttgtagag cctcccgtaa ttttaaactt ttataatata tatagttcaa attta
//
LOCUS       DAGCYL010000108         9302 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.109, whole genome shotgun sequence.
ACCESSION   DAGCYL010000108 DAGCYL010000000
VERSION     DAGCYL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.109"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            59..253
                     /locus_tag="MB216_001942"
     CDS             59..253
                     /locus_tag="MB216_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526742.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            522..821
                     /locus_tag="MB216_001943"
     CDS             522..821
                     /locus_tag="MB216_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526743.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(918..1352)
                     /locus_tag="MB216_001944"
     CDS             complement(918..1352)
                     /locus_tag="MB216_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4526744.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1584..2294)
                     /locus_tag="MB216_001945"
     CDS             complement(1584..2294)
                     /locus_tag="MB216_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBT4526745.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2284..3144)
                     /locus_tag="MB216_001946"
     CDS             complement(2284..3144)
                     /locus_tag="MB216_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBT4526746.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3149..3787)
                     /locus_tag="MB216_001947"
     CDS             complement(3149..3787)
                     /locus_tag="MB216_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBT4526747.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3802..4101)
                     /locus_tag="MB216_001948"
     CDS             complement(3802..4101)
                     /locus_tag="MB216_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4526748.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4144..5628)
                     /locus_tag="MB216_001949"
     CDS             complement(4144..5628)
                     /locus_tag="MB216_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBT4526749.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5648..6109)
                     /locus_tag="MB216_001950"
     CDS             complement(5648..6109)
                     /locus_tag="MB216_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4526750.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6127..7194)
                     /gene="ulaG"
                     /locus_tag="MB216_001951"
     CDS             complement(6127..7194)
                     /gene="ulaG"
                     /locus_tag="MB216_001951"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBT4526751.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7412..8182
                     /locus_tag="MB216_001952"
     CDS             7412..8182
                     /locus_tag="MB216_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4526752.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8455..9282)
                     /locus_tag="MB216_001953"
     CDS             complement(8455..9282)
                     /locus_tag="MB216_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4526753.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2877 a         1917 c         1471 g         3037 t
ORIGIN      
        1 attttcactt acatatattt gaatattaac taaaaacagc aagaaagaag aggatcaaat
       61 gtattatgta gaagtaaaaa caaaaggcgt caaaaacaaa caatatgtga aaggtatcag
      121 taacgaatat cctttacttg gttcatggaa agaagctgct cctttttcca aaccatgtgc
      181 cataaaaata aagaacgagc tagaaaaaga actgacttgt ggtaaagctg ttgtagatat
      241 catcgaaaag taaatccaat gtatagaaag gaagatcaca catgaaaagc gacgtacaaa
      301 ttgctaagat tatagaatta gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa
      361 tgtttgtata ccatatgatt atttcaatat gtatcctgga attggagatc tcgtagagct
      421 atatcaagat gaatcaaata tacttgtcat tcatcatgaa atatagagac ttataaaatt
      481 gtttattttt ataatgtatc agtatagaaa ggaaataaaa catggattat actgttgaat
      541 ttgacagtga actacttaag caatttccac aagaagaaca tttcatgtat aaagaagcaa
      601 tttcccacaa agtgacagag tatttggagc ataatttttt tcgtataaag ccagtgcgta
      661 ctcgttcggc aaaaggaata tacgagatga aagtcaaaat taataaaaaa aattatcgca
      721 tagcatttgc tttaaaaaaa aatcatgtgt tcatttttta tattagttcg aatttgcaaa
      781 aaattgtatt tgataaagag gtgagtaaaa aaataaaata gctgagcatt ggaggataga
      841 ttgagtgata ctaagtctat cctcttgttt ttctaaagaa ctaagctaga attttttctt
      901 ttgtacgtta ttatatgtta tttgtcatta ccaaaaagca ccttttttct tctagtatct
      961 atgacataag aaataccacc catttccttt acttgaacaa atcctactga ttcataaaat
     1021 ttttttgttt tctcagtatc atctgtcaat aattcgattt ggactggtcc ataactagtg
     1081 atcttctcca aagcttcatt caaaagcact ttgcctattc cttggcgctg atacctcgga
     1141 tgaatgagga tatcttgaat aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt
     1201 ccgataatct tcccttcccc attacgcttt atcagagatt cagattgttc aaatgctcta
     1261 gctaaccttg cagtgtcttt tgtgtaattc gtccaaccaa cgctcttata taattcagaa
     1321 atctcatcga tcataaggga atcaatattc atttttttct tcctttctta tacaattctc
     1381 cctctaataa tacagttctt tgactattta atctatcaca tataactctc ctgtaaagat
     1441 tctctcgatt ccttaaaatt cctctttcct tttagaatgc atactttttg ataaataaaa
     1501 aggttgtgat aaattttctt cacaacctct gtttctctct atcaagtgtt atcttttaat
     1561 tttctaccct taccctagca tctctattgc ccgtagtatg catactttcc atgtttgcga
     1621 aaataatgtt tatctagtaa atattgcggg atttcacata tattggggtt gatcagctcg
     1681 ttttctttgg ccatcagaca aatttcatct aagatcaaac tgttttctac tgctttcata
     1741 ggtgaatccc cccatgtgaa cggtccatgt ccatagacaa gtactccagg gacttcgttt
     1801 ggatctaatt ttctttcctt aaacgtctcg acaatcacat tccctgtatg aacttcgtaa
     1861 gcttctctta cttcttcttt cgttaattgg cgtgtacaag gaaccttacc ataaaaagca
     1921 tcagcatgag tcgtgccata agctggcaaa tcgcgtcctg cttgcgccca catgacggag
     1981 tgcgtggaat gagtatgagt aatagcagtt atatcttcaa aagtttgata caaaacaaca
     2041 tgtgtgggga gatctgaaga tggttttaag ctatcttctt ctattatatt tcccgacaaa
     2101 tctgttacca ccatttgatc tgcttgcatg cattcatatc tgacaccgct tggcttaatg
     2161 acgatgactc ctagctcacg gtccacttca ctcacattgc cccaagtcaa tttgactaat
     2221 ccgtatcgcg gcaatgccag atttgcttca aggacacgtt gcttcattgc tgcaattttt
     2281 tcttcacaag acatcatagc cagcctcctt caatttagaa cttaagtagt cttttgcctg
     2341 ctggatttct cttcgaaagt cttggctttt ttcactccac atttcaatca gaaatgttcc
     2401 atcataatct agacgtttca aatgtcttaa taatccaaga aaatcaacac aaccgctacc
     2461 aaaaggaaca tcacgaaact ggcctgaata ttcttttgtt acagggtaag tatcttttaa
     2521 gtggatagca gtgatataat cgatcccttt ctcaagttct cgagccggat tattttccgg
     2581 ccatgcggac aaattgccta ggtctggata tacttgtaga aaaggtgaat gaatctgttc
     2641 tttgatttct aaaaattttg aaatggagtt cataaatgga tcatccatga tttccacgga
     2701 aagaatgatg ccatatttag aagcttcctt tacgctttct tttattcctt cgataaagtc
     2761 ttctctggaa ttcattgatt tctcttcata ataaacatcg tatccagcta tttggattac
     2821 tttgacgctt agattatgcg ccaaatggat tgcttttttc attagttgtt tcgctgcttt
     2881 tttcttttct ggatcagcag aaccaaacgg aaatcgccga tgcgcactta aacaaatcga
     2941 atggatacgt acaccggagc tgaacatctc ttctctcagc tcagctatct tttcttcaga
     3001 ccaatctaat cgtgctaacc gctcatccgt ctcatcaatc gacatctcga taaaatcgaa
     3061 gtccatttct tttacaaggg aaaaacgttc tttccatgaa atatctttgg gtaacgcttt
     3121 ttcataaatc ccaatttgag ccatttcctt acccccagat acgaatgatc tcatctttga
     3181 agttttgtgc ggccttcatc ggatcatttg ctgctgtaat accacgacca gtgataaagg
     3241 tatacacatc gatcccttca aataagtgta atgtgtgtgg atttaatcca ccagttacag
     3301 aaacattaaa gcctagttca atcagttttt tgatcttcga taaatctttt tcaccccaag
     3361 tttcaccaga taacagcgca tcacggcttt gatggtagat tacctgccgg ataccgatgt
     3421 tatgccaatc catggcttgt tcaaaagtcc aattgccgta gagttctact tgcaattcac
     3481 ctatttcttt ttgtgctgcc tccatagttg gaatcgttgc acaacaaatg actgtcatcc
     3541 agtctgctcc tgcttttgca acattactcg ccactgttcc tccagcgtct gcacatttcg
     3601 tatcagcgac tagtttttta tctgggaaca tactgcgaat acagcgaatc gctttttgtc
     3661 cttcctgtaa gcaaagaatt gtgcccactt caataatatc tactacatca ccaactttca
     3721 taacatctcc taatgcatgt tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc
     3781 tgctcatctt agttccttcc tttacttgtt tttttggtac cactttgttt cagcaaattt
     3841 ttcactgatt tctttgactg acatcacatt tttgataccg ataactgtaa ctcctttttt
     3901 ctctgcctga gtaaacatat tcaaaaaatt gactggcgta aaaactacat cgtattggcc
     3961 agctgaactt tttccctctg aaatggcaca atgatggatt ttggtaattg gtacgtttag
     4021 ttctttcatt gctttttcca cacttctcat catcatcaag cttgttcctg atccatttgc
     4081 acacgatact aaaattctca tcttaatccc tccattatga gtgttgtcta atcagatttt
     4141 cttttatact ctctgctttt ctgcatatgt gtcatagtct tctacaatca aaaaataacc
     4201 atcaggattt ttccgatatt gtaactgcgg aatagccaat aaaccaatga caactacggc
     4261 aattccagca taacctagga ctttcatgat tactgtaaac aatggccaag ctgttgccca
     4321 gtcgaacatc ccgatataac caccgtatgt tgacatccca atccatgtag cgaataatgc
     4381 tgaaccgcct acttgtatga ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc
     4441 acctcggtta ttggcataaa ctgctataac ggcattatca aagaacaacg gaatgaaacc
     4501 ggctatgacg atcgttggtg atttcaataa aatcagtaaa ccaatcatca aaaactgtcc
     4561 caaagcacca aacaagaacc caatcgttac cgcatttggc gaaccaaagc caaaagtagc
     4621 agctacgtca attcctggaa cagctcctgg taaaatcgtg tttgagattc cttggaaaga
     4681 ttgagttagt tcatcgacga atgtccgaac acctaattgc aagatcgcca gatataccgc
     4741 aaaatttaaa gaagtcgtca taatgtagaa taagaaactc tgcccttctt gcatgaattg
     4801 cgcttgaatc aaataatctt tgcctagtac aatcagaatg atcccaaaga aaaatagcat
     4861 gagtaacgaa gtagcaacca tattctcatt gaaaatcgac aagaatcctg gtaatttgat
     4921 atcttcaatt tttttgtctt gcttgattga tctatttttc ttttcatccc gctttttcat
     4981 ccactcagct aattttgcaa atataaagat tccgaacatt tgctgatgtg ccactgcaaa
     5041 tccggcacct tctgtcaatt cctgcgtgat tcctactgtt aagttagaag ctactgccca
     5101 ataacaacct aaaatcagtc ccataaaaat caatacttgt atcctaccta gatcaggaaa
     5161 gcaaaacagt aaaatccaaa aagcggtagc tgcttgttga acctgaacgt ttccggtagt
     5221 gaaaacggaa cgcagtttcg tatatttctg aaaacggact aataagatat ttaaaacaaa
     5281 tgcaatcagt aataaaatca tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc
     5341 agcatccaca gcattttgtc caaaataagg atctgtaacc atggcatcca aattgaaccg
     5401 ttcctttagt ccaaccaaga ttggccgaaa attattgacc aaaccaccag agccaacagt
     5461 caaaataaag taaccgaccg ttgcctttaa gaatccagcg atgcattcat gaagcgggcg
     5521 tttcagcaaa atatagccta ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa
     5581 gatatttgtt gcaaaatacg tccaaatgct taacaaaaat tcccccatct tttttctcct
     5641 ctatcaatta attatattct gccatcacaa cttggtaatc atcgatggag ttcgtagaga
     5701 tgagacgctc aaccaatcca tctgtcatta ataattcgga taatttttgg atattttcta
     5761 gatgttcttc tggatctttt gctgcgagtg taaaaaacaa actagccgtt ttctcctcat
     5821 tctccccttc actttcaaaa cggattggct gtttcaactt ggtaaatgaa atagctgttc
     5881 caaaaacacc atcacttttt tctgaagaat gcggcattgc tacttgaggt gcgatcacaa
     5941 tataaggacc gtatttttct acacaatgga tgatctcctc cacatacgtt tcatcaataa
     6001 tttccttatc gatcaataca agacagcttt cggcgatagc ttcacgccaa tccgtgattt
     6061 ctttatctgg gtaatgaatc aattgatggt caaaaaagta tttcagcaag aaactcccct
     6121 tctttcttat aaaaacgacg gaaacggtgt atcattttca atcgagaaga catcttcaaa
     6181 tcctcgataa aaattaaact caagatcatc tttgtttgtc ggatagatga atttcccgcc
     6241 tacttgccag atataaggtt taaatccata cttcaggcga tcctttttga atttccaaat
     6301 ttcagtgatc tcttttggat cagccataaa attagaccaa atatcgtagt ggacaggaat
     6361 cactactttt gcattcaatg actccgccat gcgcagcata tcaacagaag taactttgtc
     6421 tgttatccct cgtgggtttt caccataggc ccctaaacag acatcgattt tgtgttcatt
     6481 accatgtttg gcaaataaat tggagtaatg tgaatctcct gcatgataaa gattccctcc
     6541 tgatgtttcg aataaatagt ttactgcaat ttgatccatc tcctgaggca tttttccttt
     6601 taatacctct tctggatctt cacaagtgac caacgctgtc cgatcaaacg cttctaaagc
     6661 tactacattg atgtctttga ttttgactgc atcacctggc tttactgtaa tcgttttttc
     6721 ttcaggaatt ccccattttt gccacgtgcg aaccacttct tttggcccaa tgaatgttgc
     6781 ttcaggacaa ttttgatgaa ctgctgcggc tgtatggata tctagatgat cggaatggat
     6841 atgtgtaacg attaaagcgt ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg
     6901 agtacgtaaa ttaggctgca tattttgaac gccactcata cgcatcatct gatggccttt
     6961 tttcatcatg ccattcccat gactacgttt tcccgtccca caccatagat cacataaaat
     7021 attcgttcct tcatgacttt tcagccaaat tcccgtacag cccagccacc acatagcaaa
     7081 agttccagga tccactactt ctgtttcgat ttcttcattc aagtatgtgc cccactcagg
     7141 aaatgtttct actaaccact tttcttttgt tacttcattg acagttttac tcatttgctt
     7201 gctcccccca tatcgttgtt ccaacttaca agtaaatatt aatgtgtatc agcatccatg
     7261 aaaacccttt tatatgaccg tttgtttttt atatgatttt ctttcagtaa tcaatgattg
     7321 tttcagtata ataaagacag atatgaatgt ttaaatgaac aactagtcaa ataaatccat
     7381 ggatcaagat gtccctcagg aggcaaaagt catgaaaaac tcacttgtca atattgaaaa
     7441 acgtcaaaaa gaaattttgg aactgcttca gcagactgaa caactatcaa ctttcgaatt
     7501 agctgaatcc cttgatgtat ctattagtac gatacgaaga gatttaaatt tattagaaga
     7561 aaaaaacgag atcatacgca attacggtta ttgttcttat aattacaaaa accagactga
     7621 ttttgatcaa tctggtcctg aaaggattaa acaagcgatt gccagagaag ctagtaaata
     7681 cattaacgat tatgatacgc tgtttatcaa ttctagctcg actgcgttga aaacactgga
     7741 ttttcttcaa tctaaccatt tgacagtcat tacaaacaac gtgaaaatca attactctcc
     7801 gcacaaagat aattgtagtt atattttaac tggtggagag atgcgctttc caaaagaagc
     7861 actagtgggc gacatcgcat taaatacgat ttcttcagtc aatgcagaca tttgtatcat
     7921 tggctgtagc ggagtcgatg tagtacatgg ggtcacaaca aagatattaa atgaatcaac
     7981 gatcaacgaa atgatggtca acaagacaat caaatgtaag attttagtcg ctgatcatcg
     8041 aaaaatcgga ctgacatcaa agttcaagat tgctgatatt tcagtctttg attatttgat
     8101 cactgataaa ttttgttctg aaaaagtatt agaggagatc agaaatacgg ggataaaaac
     8161 cattcaagta gattcatttt aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa
     8221 aatgaataac aaatttatcg ataaatgaaa aacacgctac gctcaaattg ataaaggaat
     8281 cccaagatgt atccatccaa tcgataccat cataatctat tgcttctttt ataaccgtta
     8341 tatgttgcct aaataggaat caaacaatat cgaaaaaagg aggtatgaca aaagtcacac
     8401 ctcctttttt cgaataaacg gtattcaaat cagaacactg atttcacaac cactttattt
     8461 caggtcgttt tcaatcacct ttaccgcgta atcatttccg ttgaaacgcg ctattttttt
     8521 tgcgtccata tatctttcta aataatcatc ttctcctaat tgcttatggc aagaagcaat
     8581 ttcataaagc aagtattcca atgcataagt cgatttatgt ttcttattat actcaatacc
     8641 cttctcacat aattggattg ctttatcata atcctcgatt ttacggtaat aggtggcgat
     8701 atcataatat acaatgcaga ccatcagatt atcaaaatca gtctcactca atattttaag
     8761 tgtacgctcc acataaactt tcgcaaaatc taattctcct tttgacaagt agatcattgc
     8821 cacgtttgca gctgcttgta agtgatggat atctgtttcg gtcgtttgtt ccaacacttg
     8881 attaaaataa aataatgctg catctggttt tttcgcataa agatgatcaa gcagtccttg
     8941 ataaaataaa atttgattta aaagatcaga attacttact ttttcctgat cgatcgtttg
     9001 taaaatttta taagattctt ggtattttga tacagaattc aatgattgaa tctcatttat
     9061 ttttttcctg attttgtctt cttctgtttc tactaatata tcggagatct cgacattcag
     9121 ctttttgcaa agcgcactta agatattgat gcctggcgac atattcttgt tttcgatatt
     9181 gctgacagtt gcttgttcac aaatgccttc tgctaattgt acttgtgtta accccttttt
     9241 ctttcggtat tcttttagta atgctccatc tactttaatc aaaacaattt ccttcccttt
     9301 aa
//
LOCUS       DAGCYL010000109         9295 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.110, whole genome shotgun sequence.
ACCESSION   DAGCYL010000109 DAGCYL010000000
VERSION     DAGCYL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.110"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(26..694)
                     /locus_tag="MB216_001954"
     CDS             complement(26..694)
                     /locus_tag="MB216_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4526754.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            828..1115
                     /locus_tag="MB216_001955"
     CDS             828..1115
                     /locus_tag="MB216_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526755.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1531..3069
                     /locus_tag="MB216_001956"
     CDS             1531..3069
                     /locus_tag="MB216_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBT4526756.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3066..4031
                     /locus_tag="MB216_001957"
     CDS             3066..4031
                     /locus_tag="MB216_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4526757.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4018..5340
                     /locus_tag="MB216_001958"
     CDS             4018..5340
                     /locus_tag="MB216_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4526758.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5403..5507
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5581..6324
                     /locus_tag="MB216_001959"
     CDS             5581..6324
                     /locus_tag="MB216_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4526759.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6339..7331
                     /locus_tag="MB216_001960"
     CDS             6339..7331
                     /locus_tag="MB216_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4526760.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7343..8080
                     /locus_tag="MB216_001961"
     CDS             7343..8080
                     /locus_tag="MB216_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4526761.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8070..8792
                     /locus_tag="MB216_001962"
     CDS             8070..8792
                     /locus_tag="MB216_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBT4526762.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8876..>9295)
                     /locus_tag="MB216_001963"
     CDS             complement(8876..>9295)
                     /locus_tag="MB216_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBT4526763.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3045 a         1603 c         1965 g         2682 t
ORIGIN      
        1 taaataaagt agtgaaaaat aagaatcagt gctctaactg ttcaagagga tcaagtggtt
       61 caaatgtttc tgtagtctgt tttgcttcca attggggcaa aacagatcca atttccagat
      121 acttcacttt attcaatgta taagatagaa tagaacagct aagtaatgta tacagaaaat
      181 gtgtgagatc caaataaagc aaggagacaa ctaaaatacc aaaagtaatc aagaaaagac
      241 ttttgccaat cttaatatga aaatattttt caatggcttg ataaatgata tctgttccgc
      301 ctgtgcttcc gccgaatcga aagacgagac cgcaaccaat gccagaaaga atacctgtca
      361 tcaaactatt gatcagtaga ctatctacag caaaatgtat atgaaggctc tcccataaat
      421 gtaaaaagaa aactagcgca aatgttccga cgatcgtccg gagaaaagcg tgttttccaa
      481 aaagaaacaa tgagataagc aataaaggta aattaaaaag cagtgaagtg acagaaggag
      541 cgaatccaaa aagattatgc aaaagcaaag caataccagt aaccccacca tctgccagct
      601 tagatggaat attgattcct gtcacagcta atccgtagat tcctgcgcct aaaatgattc
      661 cgcacaattc tgtaaataaa tttaattttt tcatttctag cctcttttct tttgaaaatt
      721 ttcagcaact tctttattat actgacttat actcttactt caagaggaaa atgaaaaaaa
      781 attatttttg gctgtttctt tttatttatc aaataaggag tggaaaaatg cgagcagcag
      841 acttattgac aattcttaca aaatcaccca aaaaaacaga gtatcaagga gtttttttgg
      901 ctcaagaaca aaaactggtg cctgtgacaa tggtccagat aaataaagca aatcaattta
      961 tctttttatt tgaacacggc aaagcaccac ttgctatgaa agacatcttt ctttttttga
     1021 tgaagaaccg tgagaaagct gtttattatc agtcaaaaga agaactcatt cctctttatg
     1081 gagtgaaaga gatagaaaat aaagtggtga tataagtaaa aataagaaaa caagacgttt
     1141 atgatgtcaa aacaaccatt ttttacattt ttggcaaaaa agtgtcatga tacagtataa
     1201 tattcgttga acaggccaat aaaggtgttc tcccccacga gatttctact ttattggctg
     1261 tttcttttta ttaaaataaa agttgtgtgt gtaaaaaaga tatagctgat tccaagtgac
     1321 aatttcgtat agatttttat ggtattcgta gtgaaaaaac gagaaccaaa aagttgatcc
     1381 gactttgttg cttggattct ttttttgtca gcatatttag aggtattttt ttcttttttt
     1441 tgttcgtgct gtaaggttaa ggcaagacaa gttagttggg aaaaaggaaa gaaatgttta
     1501 tacttaaaga ataacagaaa ggaaggtcgc atgaataatt ttatacaaac attaagtgaa
     1561 agaaaaggcg agttgctgga agctgcattt caacatctgt ccatttctct catttctttg
     1621 ctgattgcag cactgattgc tattccttta gcgatttggg cagccaatca taaaaaaatg
     1681 gcagaagtct tattgcagat cacgggtgtg ctccaaacga ttccttcact tgccttatta
     1741 ggtttactta tcccatttgt agggatcgga actgttccag cattgattgc acttgttatt
     1801 tacgctttgc ttccgatttt ccaaaacacg tatattggtc tagctgaaat tgacccgtct
     1861 attgaagaag cagcggtagc atttggtatg tccagaatga gacgcttgct gaaagtagag
     1921 cttcccatcg cattacctgt cattatctca ggtatccgaa ctgccttagt tttaatcatc
     1981 ggtacggcta ctttggcagc attgatcgga gcaggtggat tagggacatt tattttgtta
     2041 ggtattgacc gaaatacacc tgttttgaca ttgattggtg cgatcagttc tgccttgctt
     2101 gcaattatat tcagcggact gattcgctgg ttgcagcata agaaacctcg atattccatc
     2161 attactttag cagcagtggt gtgtttattt ggcggcgttt ctttatatca gtctgccttg
     2221 tttaaaaatg aaaccatcac gattgctgga aaattaggag cggaacctga aatcctgatc
     2281 caaatgtata aagaattgat tgaagacaac acaaaaacag aagttgaatt aaaaccgaat
     2341 tttggcaaga cgagtttttt gttcaatgca ctggatcaag gtcaaatcga tatctatcct
     2401 gagttcacag gtactgtcct agaaagcttg gtgaaggtac ctgaagcaat gaaaagtgct
     2461 gatttgacaa aagaaaaaac atatgacgaa gcagaagaat tattgaataa gcaattcgat
     2521 atgacattat taaaaccaat ggcttaccaa aatacgtatg ccttggcagt caagagatct
     2581 tttgcgaacg aacatcaact taaaaagatt tccgatttgg cagctatctc tgatcagatc
     2641 aaagcgggat tcacactgga atttattgat cgttcagatg gatataaagg aatccaagaa
     2701 gcatatggct tgaatttttc aagtgtacaa agcatggagc caagtttgcg ttaccaagca
     2761 atcaataatg gagatgtcaa tattgtggat gcctattcca cagatagcca gctcaaggag
     2821 tacgatctag tgactctaga agatgatcga gggttattcc cagcttatca aggcgcaccg
     2881 ctgatgaaaa aagattttgc taaagaacat cctgaaatcg ttaaagcact gaataaatta
     2941 agcggaaaaa taacagagga tcaaatgatt gatatgaatt accaagtcaa tgtcgaaaag
     3001 aaaaagccga cagaagtagc acgaatcttc ctgaaaaatg aaggattagt aaaggaggaa
     3061 gatcagtgac taaattaatc gagttcaaac atgtgcaaaa aaagtatgat ggaaagtatg
     3121 tcatcgatga cctaaatctg gcaatcaaaa aaggagaaat ctttgttttg gttggtcctt
     3181 ccggtagtgg aaaaacaact actttgaaaa tgatcaatgg attatctaaa ccatcagcag
     3241 gagacatcta cttcaaagga aaatcgttaa atgaatataa tctgcagaaa atgcggtgga
     3301 atatgggata tgtgctgcag caaatcgctt tgttcccgac catgacagtc aagcaaaata
     3361 ttgaagtcat ccctgaaatg ttaggttggg aaaagcagaa aagagcagac cgtgtagatg
     3421 aacttcttca aaaagttggc ttatctcctg atatttatcg agatcgtatg cctagagagt
     3481 tgtctggagg agaacagcaa cggatcggga tcatccgagc tattgctgca agcccagatg
     3541 tgattttgat ggacgagcca tttagcgcat tagatccgat ttcaagaaac tcattacaag
     3601 aattggtttt gtccttacac gaagaattgg gcacgaccat cgttttcgtt acgcacaata
     3661 tggaagaagc aatcaagcta ggtgatcgta ttgcatttat gaaggatgga gaaattatcc
     3721 aatgtgacac accagagcag ttattgatga atcctaaaaa cgactacgtt cggcatttct
     3781 ttgatgaacc gaaacagacg aaagagtggc gagtagaaga tttagtcgtc aatggatatt
     3841 tcttaaatga aataccagaa aatgctcgtc aacaagtttg ctttgatacg cctatgaaag
     3901 aagtatattc gcttctttcg gtgtatccaa gcattgtgat agtagaaaag caaagaagta
     3961 ttgggtcgct gacaagtaaa gaaatctttg cttttttaag tcgagaggag gaaggaaatg
     4021 aaaacatatg atgcaatcat catcggttca ggtgtgagcg gcttaagtgc tgcttatgga
     4081 ttgaaagaag caggtaaaac agtactagtg gtcgaagaag atctatgggg cgggacttgt
     4141 cctaaccgtg gatgtgatcc taaaaaagtc ttgttaagtg cagtagaggc acgtaatcgg
     4201 gtaaaacaat tgagtggaaa aggttttaac gagatcccaa ctgccaactg ggaagaactt
     4261 caaaaattca aacgaacatt tacggatcca gtcccagaaa gccgaaaaaa acaattagca
     4321 gaagctgaaa tcgatcatct ttcaggaacc gctcgttttt tggatgattc atctattgaa
     4381 gtcaacgaag aagtcttcca tgcggattac ttagttttgg caaccgggca aagacctacg
     4441 atcttaccag tggagggaaa ggaatacttg aaaactagtg cagacttttt atcattgcct
     4501 gtactgccta aagaaatcat ttttatcggt ggtggttata ttgcttttga gctagctacg
     4561 attgccaatg ctgcagggag caaagtaacc attgtccatc ataatcaacg gccattaaaa
     4621 gaatttgagg cgtctttggt agaggaagcc gtccatcaaa tggaagcatc tggcattcag
     4681 tttgcttttg gtgttgaaac ccaaaagatc atttcagaag gtactcgcta tcgtttagtc
     4741 ggaaaagaga cggaattagt agcagatatg attttttgtg cgactgggag acagccgaat
     4801 acagaatctt tagcacttga gcaagcgaat atcgtctttg ataaacacgg aattgcagtc
     4861 aatgattatc tgcaaacgag caatccaaaa atttttgcgt gtggagatat tgtttcaagg
     4921 aaaaccccaa aattgacacc agtcgccact tttgaaggaa attatgtagc taagcggata
     4981 acggatgcaa caagcgaacc tatcaaatat ccgattatac ctacgatcgt ctacgccagt
     5041 cctaaacttg ctgaagtagg agttacaaaa agtcacgcct cttcgtcaga tcaagtggta
     5101 gagatggatc ttaccagttg gttcacctat catcgtgtca atgaaccagt tgctaaggct
     5161 gaattgacct ttgaccagca aaattatttg attggtgctg cagtgattag cgagcaagca
     5221 gacgaattga tcgatgattt gacactcgtc atcaatcaaa aattgactaa aaaagagttg
     5281 gatagctata tcatggggta tccgacattg gcaagtgatc tctcctattt attgaaataa
     5341 tcagcatgat aactaaagcg cttgttgctt tttccttctt gtgctacact ttaaaaaatg
     5401 aatacctgtt tctggggatg tcgtagacga catgagagta agttgtgaac ttagacccat
     5461 tgaacctgtc agttaatact ggtgaaggga gaaacatagc agacgcttga agcgggttac
     5521 tatgtcgtgc ccatttcaag cgttttttgt tattcatatt agaagaaagg aggtaacaaa
     5581 atgaagaaaa atctttatcc agcagtcagt atggcattca ttttaattat ttggcagatt
     5641 gttgtcatag tttggaaaat acctgccttt attttacccg gacctgctgc tgtattttct
     5701 gcattagcgg cagatgcaca agtattactt tcccattctg tcacgacatt aaatgaagca
     5761 gtgcttggtt tggtaatagc tagcgttctt tcttttatta ctgccttggc aatggattat
     5821 tggaagtttt tagaaatgtc tttttatccg ttactagtga tttcacaaat gttgccaatc
     5881 atggtgttag ggccattact taccctatgg tttggtttcg gtatgcttcc aaaagtgatc
     5941 cttgttgtct tgataagcta ttttccgatc gtagtttctt tttcggatac attgaggaaa
     6001 gtatcaaaag agcaaatcat ctttttgaaa acgatggggg cagatacagg gaagatttat
     6061 cgaatatata aaataccagc gggtttatcc ggcttctttt ccggtttaaa agtggctgct
     6121 acttattgtg tcagcggagc agtcgttgga gaatggctaa gcgctcaagc aggcttaggg
     6181 tattacatga tacgcgtaaa aaacagctac cagatcgata aagtttttgc tgcgatcatt
     6241 tgtgtcattc tattgagttt attgctgaat ggcgcttgtt cattattgaa aaaagggtat
     6301 tatcgtatgc tttatatttt ttaagagaag gggatcaaat gaaaaaatta tggcttttat
     6361 taccgctact gttgctttta tccgcttgtg gcactgcaaa agaaacaagt agcaaacaag
     6421 aaataaagga tttgaaaaaa gtgacacttg ttttggatta tgtaccgaat actaatcata
     6481 ctggcatcta cctagcaaaa gaaaaaggct attataaaga ggctggttta aatgtccaaa
     6541 tcatcgaacc aggagataat tcgacaagta ttggtctagt aggtgcggac aaagcacaat
     6601 ttggtgtaag ttatcaagaa gatgtcactt atgctcacgc agatggacag aatataccag
     6661 tcaaagcaat tgctacagtg atcaaacata atacttctgg attcgccacc ctatcagaca
     6721 gtaacatcca ttcaccaaaa gattttgaag gaaaaacata tgcaggttgg caaagtccaa
     6781 gtgaagaagc tgtcttaaaa gctgtgatgg aaaaagacgg cggggacttt tcaaaactga
     6841 caatggtagg aagcaatgga gaagggccag aaagcttagg aaaatcctca gatatccaat
     6901 ggtactttga aggttgggat atgatcaaag caaaagaagc gggtattgaa ctaaactata
     6961 tcccattgaa agaattagat gaacggttag attactatac tccggtaatt attacgaatg
     7021 atcaactgat caaaagtgat ccagaattgg tacagtcttt tatggatgcc acgaaaaaag
     7081 gctatcagga agctattaag gatccaaatg acagtgctaa actgttgcaa aaatatgcaa
     7141 aagaaaatga tcggacgttt ttagaagaat cacaagcatt cttgtcaaaa aattatacgg
     7201 atgatcctga gaattgggga ttgatggaag aaaaagtttg gcgcaattat acagctttta
     7261 tgcaggaaaa cggattgatc aagcaagatg taccttcgca agaactattc actaatcagt
     7321 ttataaaata ggagtgatag atatgtcaat ccaaatcagc cacttgaatg tagtcattga
     7381 aaacctgaaa atattagaag acatccattt cactatccct aaagatacct ttacatcgat
     7441 tgtcgcgcca agcggagccg gtaagtcaac attgctgaaa actttaacag gagtccagcc
     7501 tatgacttct ggaacgatca aagtagacga tcaaaaagta actggtctcc atacagcatt
     7561 tagttatatg ccgcaagaag atatgttact tccttggctg agcgtctatc aaaacgttac
     7621 gttatatcaa aaaatcaatc atctaacaat caatgaagaa caagtaaggg attatctgga
     7681 tatctttggg ttaaacggct atgaacattt tttgccggaa caattatctg gcgggatgaa
     7741 gcagcgaacg gccttgttac gaacaatcat gaatccttct tcttacttgc ttcttgatga
     7801 accatttgga gcgttagatg ctatgaccag agggcagatg caagactgga tgttgaagct
     7861 gcctaaaaaa gcaaagcgga ctactttact tgtgacgcat gatatcgaag aagcaattta
     7921 tttatcagac aggattcttg tgctttccgc ccgtccggca catgtgattg ctgagattca
     7981 agtgcctgaa aaacgacggt cacgtgaatg gctactgcag caatcagagt tgaagcaaac
     8041 gatttatcag ttattggcag gtgaattaga tgttaagtga tgctcattgt catttagatg
     8101 ggagtcagag tttagcactc cttcagcagg aacacacgat tttgacgatc atcaattgtg
     8161 attcgccaga agaatggaag gaaaacagac agcttgcagc aagtaagact caagccttaa
     8221 gttacggtat ccatccttgg aaagcagata gctatacgtt tgaacaagtt gagccttttt
     8281 tgaaaaaagc aaggatcatc ggtgaaatag ggttagataa tatttggacg aatgtaccga
     8341 tgactacaca aaaaaaagta tttgagcgac agcttgcttt tgcagccata aatgagaaac
     8401 ctgttgtact gcacacaaaa ggatgcgaaa aagagatctt aaattatatc caagcttacc
     8461 ctaatcacta tttgatccat tggtattctt ctttggacta tcaacaagac tatattgatt
     8521 taggctgtta tttcagcatt ggtcttgatt tgaaaaagaa tccagcagtc tggcaactag
     8581 ctaaagcagt cccaatcgac cacttactta ttgaaacgga tggactacat gcagcagagt
     8641 gggctttagg ggggattttt tcagaaaaag attatgtctc actgcttgaa gaacagttaa
     8701 agacaatcgc tgagatcaaa gagctatcag ttgaagaagt taaaaaaaat acttatcaaa
     8761 acttggaacg tttcattaga ttaggtaaat aaaaaagaga tgtgacacaa ctagtgtcgc
     8821 acctcttttt ggctttcttt gtataaaagt aaactgtttg attaataaat gaatcttagt
     8881 accaaccgtt tgcttcccag aatgctttcg ccgcttccca agaaccataa cgtgaagtaa
     8941 catattgttc tgctactctt tcttggtttg cagcagagta gtcaccattt aagtaagaag
     9001 aatctaattg gtaacgtccg atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt
     9061 ctttttgtgc gatccattct tttgcagagc ttgtgcttgc tggagtagca gcagtttctg
     9121 ttacagcagg agcaggttgt gtttcagcaa caggcgctgc ttgtttttcc gtttgaaccg
     9181 tttcttgtac aacaggttgt tcagcttgga ctggtgcttc ttgaacggct gcttgaggtt
     9241 cagcagctgc ttgtgcagag ccttctgttg ggattgttaa ttgttgtcct gagta
//
LOCUS       DAGCYL010000110         9156 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.112, whole genome shotgun sequence.
ACCESSION   DAGCYL010000110 DAGCYL010000000
VERSION     DAGCYL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.112"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            23..247
                     /gene="nrdH"
                     /locus_tag="MB216_001964"
     CDS             23..247
                     /gene="nrdH"
                     /locus_tag="MB216_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBT4526764.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            406..2565
                     /gene="nrdE"
                     /locus_tag="MB216_001965"
     CDS             406..2565
                     /gene="nrdE"
                     /locus_tag="MB216_001965"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBT4526765.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2615..3580
                     /gene="nrdF"
                     /locus_tag="MB216_001966"
     CDS             2615..3580
                     /gene="nrdF"
                     /locus_tag="MB216_001966"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBT4526766.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3669..4808)
                     /locus_tag="MB216_001967"
     CDS             complement(3669..4808)
                     /locus_tag="MB216_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4526767.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5117..5830)
                     /locus_tag="MB216_001968"
     CDS             complement(5117..5830)
                     /locus_tag="MB216_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBT4526768.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6084..6785)
                     /gene="nagB"
                     /locus_tag="MB216_001969"
     CDS             complement(6084..6785)
                     /gene="nagB"
                     /locus_tag="MB216_001969"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBT4526769.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7019..8551
                     /locus_tag="MB216_001970"
     CDS             7019..8551
                     /locus_tag="MB216_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBT4526770.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8742..8921)
                     /locus_tag="MB216_001971"
                     /pseudo
     CDS             complement(8742..8921)
                     /locus_tag="MB216_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(8941..>9156)
                     /locus_tag="MB216_001972"
     CDS             complement(8941..>9156)
                     /locus_tag="MB216_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBT4526771.1"
                     /translation="SEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFE
                     SILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         3046 a         1787 c         1612 g         2711 t
ORIGIN      
        1 tgtgttagag gaggcactca ccatgaacgt aaaaatcttt tctaaaaata attgtatgca
       61 atgtaagatg gtcaaacggt ttttaacaga aaatcaaatt gcttttgaag aagtcaatat
      121 cgatgaacag cctgaagcag tagactggtt gaaagcacaa ggattccaaa gtgttcctgt
      181 cattacttct gatgcagaaa cagttatcgg attccgtcct gatcagttgc gtaaacttgc
      241 cagctaacat gactaaaaga atcaaaacgt gatacatgga tttgaagggc ttgagacagc
      301 accagttttt gtcgcaaact tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt
      361 tgcaatcaag catcatcaaa tacgaaatga aggttgggcg tacgtatgag tcttaaaaat
      421 ttacaagatg tcagttattt caagctgaat aatgaaatca atcggccagt caatggtcaa
      481 atccctttga ataaggataa agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca
      541 aatacgatga cttttccatc tattatggaa aaaatcaatt acttgatcga tcaagaatat
      601 atagaagcag aattcattca attgtattca ccatcattta ttgaaaagct atatcaattt
      661 ttggacgagc aacatttttc tttcaaatct tttatggctg cttacaaatt ttattctcaa
      721 tatgcgttga aaaccaacga cggttccgct tatctggaaa cttatgaaga tcgagtagcc
      781 ttcaatgcgc tttactttgc gaacggggat gaagaattag cacttgctct tgcagacgaa
      841 atgatccatc aacgatatca gccagctaca ccttctttct tgaatgctgg acgtaaacgt
      901 cggggcgaat tggtgtcttg tttcttgact caagtcactg acgatatgaa ctctatcggg
      961 cgctcaatca actctgcttt gcaattgtcc cgtattggtg gcggcgtagg gattacttta
     1021 tcaaatcttc gtgaagccgg cgctcctatc aaaggatatg agggtgctgc aagtggtgtc
     1081 gttccagtca tgaaattatt cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt
     1141 caaggagctg gcgtggttta tttgaacgtt ttccatccag acattattgc tttcctttca
     1201 gcaaaaaaag aaaatgcaga tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc
     1261 gtacctgata aattttatga attgacacgt aaaaacgaag atatgtattt atttagtcca
     1321 tatagtgtcg aaaaagaata tggtgtccca ttctcttatg taaatattac agctgagtat
     1381 gatcatctag ttgcaaatcc aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa
     1441 aatgaaatct caaaattaca acaagaatct ggctatcctt acgtaatcaa tattgataca
     1501 gcgaaccgcg agaatccaat cgatggcaag atcatcatga gtaatttatg ttcagagatt
     1561 ttacaagttc aaacgccttc tgtgattaac ggcaaacagg aatatgaagt attaggaaca
     1621 gatatctcat gtaacttagg ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt
     1681 ggtcaatctg tgcgtacgat gactcgtgca ttgacattta ttacagatgc atctgatatc
     1741 gatgtcgtac caccgatcca aaacggaaat aagctgaatc atacgattgg attaggtgcc
     1801 atgggcttac acacttatct tgcaaaagaa caaatggaat atggctcaga ggattcatta
     1861 gactttacaa atatttactt catgctgttg aattattgga cacttgttga aagcaacaat
     1921 atcgctcgtg aaagaaaaca agttttccat aattttgaaa aatcagctta tgctgacggc
     1981 acttatttcg aaaaatacgt aactggagaa ttccagccaa aatcagaaaa agtcaaagca
     2041 ttgtttgaag gcatttttat cccaactgct gaagattgga atgctttgaa acaagcagta
     2101 atgaaagatg gactgtatca tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca
     2161 tatatcaacg atacgagtgc aagtattcat cctatcactc gcatgatcga agaacgccaa
     2221 gagaagaaaa tcggtaaaat ttattaccca gcgccttatc tatccaatga gacgattccg
     2281 tattacactt ctgcttatga tatggacatg cgtaaagtaa tcgatgttta tgcaacggca
     2341 caacaacacg tcgatcaagg catgagtttg actttattca tgcgttctga aatcccagaa
     2401 ggcatttatg aatggaagac aacaacgaag caaacgacac gtgatttgaa tattttaaga
     2461 cattacgctt tccacaaagg aatcaaatcc atttattacg tacgtacctt tactgatgat
     2521 gcagaagaaa tcggaagcaa tcaatgtgaa agctgcgtga tctaatatca tagatagatt
     2581 cgcttattca tattttaatt aaaggagcat tctcatgtca gaaacatatt atgaagcaat
     2641 aaactggaat gaaattgaag atatcattga taaatccact tgggagaagc tgacagagca
     2701 attttggcta gatacacgta tcccactttc aaatgaccta gatgattggc gtacattgtc
     2761 tcaattggaa aaagatacag taggacatgt atttggcggt ttgacactgc tagatactgt
     2821 ccaatctgaa agtggcatgg atcagttgcg taaagatgtc cgcactcccc atgaagaagc
     2881 cgttttgaac aatattcaat tcatggaatc cgttcatgcc aaaagttatt cttctatttt
     2941 tagtacactg aatacgaaaa aagaaatcga agaaattttt gactggacaa atacgaatcc
     3001 ttatctgcaa aaaaaagctg agcgtatcaa tgaaatctac aaacacggta caccgctaga
     3061 aaagaaaatt gccagcgtct ttttagaaac attcttgttc tattcaggtt tttatactcc
     3121 tctttattat ttaggaaaca acaaattggc taacgtagca gagatcatca aactaatcat
     3181 ccgagatgaa tctgtacatg gaacctacat cggttacaaa ttccaattag gattcaacga
     3241 gttacctgaa gcagaacaag aaacgttaaa agactggatg tacaatcttc tttatgagct
     3301 ttacgaaaac gaagaacgtt atacagaaga attatacgat ccaatcggct ggacagaaga
     3361 agtcaagaca ttcctacgct ataatgccaa caaagcattg atgaacctag ggatggatcc
     3421 actctttcca gatactgcca atgatgtcaa cccaatcgta atgaacggga tttcaacagg
     3481 cacaagcaac catgacttct tctcacaagt cggaaacggc tatttgttag gtcatgtaga
     3541 agcgatgaaa gacgacgatt atttgattgg attggaataa ggatttattt taattcaaaa
     3601 atttttcgaa aaataaaaag agtttagcgt acagatattt taaatgtaca ctgaactctt
     3661 ttttatgctt attcacttgt ttcttccagc acttttggac tgtctaactt ttccgaaaca
     3721 tgttccattc ttctcaataa atagattttc cggatattta atattatcgt cttaatcact
     3781 gcatagacag gtatgcaaat cagcatgcct aatattcctg cgacacttcc tgctccaatc
     3841 aataccaaga tgatcgtcaa aggatggata ttcattgatt tcccaaataa caacggcttg
     3901 atcaaatttc catctaattg ttggatcact aaaatcgaca acgccatata caaagcttga
     3961 aatggagaga taaacaaacc gacaatgatt gctggtaccg ctccaataaa agggccgaca
     4021 tacgggataa tattcgtgat tccgcaaatg accgcaagca acaagctata aggctgatcg
     4081 aatattctca ttgccaaaaa gctcattaca ccaataatca aagcatctaa gatcgtactg
     4141 ctgatatagg cagataatgt ttcgtttaat tcttttaccg tttggcgtaa ctctttacga
     4201 atcgctttcg gaaagaagtg agacaacgca tcaataaatt tgtgcccatc cttgaacata
     4261 aatagcaaaa taaatggaac ggtaaataac aaaataaaaa attgcatcat aaatgaaatg
     4321 atttttgaga cacttgcaga taaactgact aatatgatat tgaacaaatt actaagagat
     4381 aaatttgctg atgctaattg ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc
     4441 aattcattta accatttgct tgtttcatct gcatacattg gcaagctctg ggtcaattga
     4501 attgtctgtt ccattaactg aggcactaca ttcatgacag ttaaaacaac taaaatgatt
     4561 acgaccaaaa acgtcaataa aaaaccgaac actctcggga tccgatgctt ttcgagaaaa
     4621 acaacgattg gatcgaacat ataatagata aaaccagcaa caagcaatgg taaaaaaatt
     4681 gccgaaagca ttttcaatat tggactaaaa atattcggca tttgtaaaat aaagtacacg
     4741 ccgatgatcg tgagtactag ctccactgtc cagaaaaata attttgattg tttaaaacgt
     4801 gattgcatac atctcctcca aggtcctttg atacttctat tctatcattt aacaaagagt
     4861 tacgcgctga gattttcatt tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag
     4921 tagacgagta cttaaacaaa aacacggttt ggagtagtga caaaaattat actgcagtcc
     4981 ctttttccaa ataaacggtg ttcaaaagct tgcatcttcg taataagccc agccaaacga
     5041 aaaatataag gaactatttt tatttggctg ggcttattac ttgatgtagg acatctattt
     5101 tacaacctca gatcatttat tgcgcaatac tgactaaatg caactttgtg atatttttgt
     5161 gtacggacaa ctcctcgatc acatcggcat aagtgagccc ttttggaaga tcaatcgtat
     5221 agatattttt gtacactcgg tagtcatcaa cgaattttac gtcaaaattc acatcttcga
     5281 ttacgatatt tttctcttca aaataatcgg taagaaaatc tttggtttct tttctatgga
     5341 taaattggat ttctaacttt ttagtcgtag gtacatggat cacgcgtttc actactgtga
     5401 gtgcaatagc aatcccgata aaactgatga tcgcaatgga atagtaaccc atgccgatgg
     5461 cgatccctaa tccagcaatt gcccataaag aagcggcagt cgtaagtccc gtcaccgatt
     5521 gtttcgtgac gatgatcgtt cccgcgccta agaaaccgac accactcacc acttgagcaa
     5581 tcagccttgc ttcatctgaa cgaatcgttc cagcaagctg tggatactct aaagcatctc
     5641 ttaatgcacc actagcgatc tcaacttgga tcaatgcaat gatacatgct cccatacaaa
     5701 ctaaaatatg cgtacgcata cctgctggac gatttttata ttgtcgttca aaaccgattg
     5761 ctccgccaaa tagcatagcg atccctaaac gtaaaataat ctctggaatt gtcaatacca
     5821 tatcattcat ctaaacaccc tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa
     5881 aagtgcaatt tttacctctt acgaaaaaag gactgtgaca aaatttttgt catagtcctt
     5941 tttatgaata aacggtgttc aatcagctga ccctcggtat tactcaacaa acggcaaaat
     6001 atggggagct atttttcctt tgtcttctct aatactcagg tcaaaacgct gatttcacaa
     6061 cctctttagc ctactattga aaattacaac ttacttgctg cttcttcatc gatgatcaca
     6121 acaacattgt cgtgattttg caatacactt gctggcaaat gattcgttac aggtccttca
     6181 atcatccctt tgattgcatc agctttgctt tcgccaaatg ccatcaagac gatttcttta
     6241 cttttcataa ttgaaccgat tcccattgaa acagcttttg ttggtacatc ttctgcttta
     6301 tcgaagaatc gtttgtttgc attgattgtt gattctgtta agtcaactac tgaagtcgtt
     6361 ccgtcaaatg aagcaccagg ttcattgaaa ccgatatgtc cattttgccc gattcctaag
     6421 atttggatat cgattggatg tgcatcgatg atttgatcat actttttgca tgctgcttcc
     6481 agatcttctg cttttccatc tggtacaaac gtttctttga atggtttttt atcaaataat
     6541 tggacatcca tgaaatgacg gtagctttgt tcatctgaac cgcctagtcc aacgtactca
     6601 tctaagttta cagaagtcat ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat
     6661 aaagtaactg gtgtgcttcc tgttgccaat cctaatactt ttgcaccttt gttcatacct
     6721 tctttgatga tttcaaatgc tttttttccg ccttcttcag cgttttttac acgaatgatt
     6781 tccatagtga ttgctccttt atattttggt atagcccaat tatagcattc tttttttctt
     6841 tttacaataa ttagtataga ccaaattgca tttttttcat tttcttgaca ataaatagga
     6901 atccgtgtta ggtttcataa gatggttcaa tcaaacaatc catgttgcat gaaaaccaag
     6961 caagtgattc actgtggacg ctttatgtct gcagtttttt tatgaaaaag gagttattat
     7021 gagtatactg acattaaaca acattaccca gcaatttggc gacaaaatcc tctatgaagg
     7081 tgtcgatctg caattaaatg ctggcgaaca tcttgggctg attggacaaa acggagccgg
     7141 taaaagtaca ttgattaaaa tcatcactgg tgagatctta ccagatgacg gacagatcca
     7201 ttggcaaaaa aatattcaca aaggttacct cgatcaatat gtggaagtcg atgaaacatt
     7261 aacaatcgaa gaatttctaa aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat
     7321 tgcttttctt taccaaacat atagcgagac cttcgatgat tccttgctgg aaaaagctgg
     7381 gaaactccaa acggaattag accaaggagt gttttatcaa atcgacacct tagttgctga
     7441 gatgagcagc gggttgggga tcgatgtctt aggtttagat tcactgttga aaaatctaag
     7501 tggtggacaa cgttcaaagg tgattttagc aaaactactt ttagaaaacc cagatgttct
     7561 gatcttagat gaaccaacca atcacttaga tgatcagcat atccaatggc tgacgcagtt
     7621 tcttcaggat tttgacggtg cgtatatcgt gatttcccat gaccaagaat ttcttgatca
     7681 gatcaccacc catatcgcag acatcgagtt tggaaagatt actaagtata ctggccatct
     7741 aaaacaagca ttgaaacaaa aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc
     7801 tcaacaaaaa caaatcgaaa agaccgaagc ttatattcga aaatacaaag ccggttcccg
     7861 atcaacgatg gcaaaaagcc gacaaaaaca attagataaa atcgaacggc tgacgcctcc
     7921 agcttcagca agtaaagctg tctttgattt tccattctcg cctattgtca cgacattagc
     7981 agtggaaaca actgatttag tcatcggata tgatcgaccg ctacttgcac ccatcaattt
     8041 gaccatccgc tttggtgaaa atattgccat tcgaggcttc aacgggattg gaaaatctac
     8101 tttattaaaa acactgatcg gtgagattga aaaaataagt ggcgatttcc acttcccaga
     8161 taacacaaaa atcaattact tttcacaaga tctgtactgg gaaaatccat tagaaacacc
     8221 actacaatat ttaagcagtc aatttccaaa agcgactatc aaagaattac gtcgccaact
     8281 agctaaagca gggttagtta atcaattagc aagtgaacct ctcactacat taagtggtgg
     8341 ggaacaaact aaggtgaaac ttgcacagtt gacaatgaac caaggtaatt tactgattct
     8401 cgatgaaccg acgaaccata tcgatcaaga aacgaaagaa agcttacaag acgggataca
     8461 gaaatttcca ggaacagtaa tcatcgtctc tcatgaacaa gaattttatc aagatcttgt
     8521 gcaacgagtc attgagattg aaccaaaata aacgtttgat tgaatagttg aaaaaaagga
     8581 gctgtgacaa aagttttgtc acaactcctt cttctgaata aatggtggcc aattagctga
     8641 ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc tatttttatt tggctattct
     8701 tattacttga tgcagaacag cttttcacaa cctcttttcg tctatttttt attttcgaga
     8761 tttctctttc ctttaaaaac aactgtttga aagaagaacg cacatccttg aaaaacaagc
     8821 ccgctaaccg ttacacctaa ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa
     8881 ccagccgcac ctccaataaa gtacataaac ataaatacag acccgtggtg ctagttaatt
     8941 ttaatatcct aaagaatatc tcaaagttcc ttcaaaacgt cgttgtgctc gacttaatag
     9001 aacactgtaa gccagtaata tgctttcaaa tctactctct agccctttaa cagaacgtga
     9061 gttgaaattt tgacaaccta atttttctaa ggaagaaaaa acagtctcaa ctgtttttct
     9121 ttgttttcca agtaagctgt ggtctagttt ttctga
//
LOCUS       DAGCYL010000111         9145 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.113, whole genome shotgun sequence.
ACCESSION   DAGCYL010000111 DAGCYL010000000
VERSION     DAGCYL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.113"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(16..840)
                     /locus_tag="MB216_001973"
     CDS             complement(16..840)
                     /locus_tag="MB216_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBT4526772.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(813..1493)
                     /locus_tag="MB216_001974"
     CDS             complement(813..1493)
                     /locus_tag="MB216_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBT4526773.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1516..2454)
                     /locus_tag="MB216_001975"
     CDS             complement(1516..2454)
                     /locus_tag="MB216_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBT4526774.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2699..3562)
                     /locus_tag="MB216_001976"
     CDS             complement(2699..3562)
                     /locus_tag="MB216_001976"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBT4526775.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3704..6277)
                     /gene="mprF"
                     /locus_tag="MB216_001977"
     CDS             complement(3704..6277)
                     /gene="mprF"
                     /locus_tag="MB216_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBT4526776.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6418..7224
                     /gene="proC"
                     /locus_tag="MB216_001978"
     CDS             6418..7224
                     /gene="proC"
                     /locus_tag="MB216_001978"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBT4526777.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7303..8859)
                     /gene="rny"
                     /locus_tag="MB216_001979"
     CDS             complement(7303..8859)
                     /gene="rny"
                     /locus_tag="MB216_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBT4526778.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         2697 a         2019 c         1573 g         2856 t
ORIGIN      
        1 tcttttcttt ataatttagt tattttttct tctgaaggca accaatattc gtttgatgat
       61 atttacaatc aggaacaaag caataaagac taatgtgatt gttgctcctg gaggtgtatc
      121 tagataaaac gagctagtta gtcctgacag catccctaga aatcctacac aaacactgat
      181 agcaatcacg acattgaagc ttttgcctaa tcgcataccg attgctgcgg gaagaaccat
      241 gattgcggat atcaaaagtg caccggcaat cgggatcatc acagcaatag ccacaccagt
      301 aatcacattg aacatcatcg acatcaaatg aacgggtagc ccatccacgt gggcggtatc
      361 ttcatcaaaa gtcagaacat acatcggccg tttgaataaa aagaacaata taccgatggc
      421 aacgaataag attcctaaaa agatgacttg ctcccatgta atggtcacga tcgaaccaaa
      481 cagataggat tggatacttg ttgctgaatt ccctccgctc aaattcatca atactaacgc
      541 tagcgccaaa ccaccagcca ttaaaatggc aatggagatt tctgaatagg ttcggtagat
      601 cgttcgcaga tactccaata tcacagctgc taatacaacg actagcatgg tcgtaagtgt
      661 aggattaagg ttcaaaaaga atcctaacgc tacccctgct aaagagacat gagaaagcgt
      721 atcagccatc agcgattgtc ttctgatcac taaaaacaca cctagcatcg gggcgattcc
      781 ggcaatgaag atagcggcaa gaaacgctct tctcatgaat tcatatgaaa gcaacgccat
      841 tgagaatcct cctttctcac cagtcggatc tgtctgtccg catactgttt gatatcttca
      901 tgatcatggg tgatcattag aactgatttc ccatggacat gcgcactatg tcggagaaga
      961 cgataaaatt catttctgga ttcttcatcc atacctgttg ttggttcatc aagaacaaat
     1021 aagtcagggt cagtagcaaa aatacgcgct aaactgattc gctgcttttg tccgccagac
     1081 aattcaccta ttcgccgatt tcgcatatcc cacatcccta cagcttctaa agcacgctgt
     1141 acatgttgat gatctttttc agagagacgc ttgaaccagc ggcctcttgg atatcttcct
     1201 gaacggacta attcgatcac cgtactagga aaacctgcat tgaaagatgc aacttgttga
     1261 ggaatataac cgatacttaa tttttttcct tcttgatttt tctcagcaat ctttatcatt
     1321 ccttttgaag gtttcagtaa tcctaacgat gctttcacca aggttgactt ggctgctcca
     1381 ttttctccag ttaaaatgac aaattcaccc gcatctacat gataagagac atcttctagt
     1441 acaggttcat catcatagta aaaggtcaaa tcagatattt cgatataacg caccttacaa
     1501 cctccattct aaaacttaat tgatactttc ttttaaagct ttcagatttt tttccatgac
     1561 agaaatataa ttttcgcctg ctttgatttg ttcattagtc aagctttcta aaggattcaa
     1621 cacttctagc ttcactcctg tttcattcga caatgtctca gctaccttcg aagaggcatt
     1681 ttcttcaaaa taaatcactt tgatgttgtt tttcttcaca aattctttta gctcagctaa
     1741 gcgacttggt gtgggttcct gatctggtga aacgcccgaa atcgctactt gttttaagtc
     1801 gtattgattc gctaaataag caaaagcagc atgctgagtg acaaatgtgc ggtttgttgc
     1861 atcttttagc tcatcggagt atttttgatc cagtgcttca agctttttga tataagcatc
     1921 ggcattcttc gtaaaagttt cctttttatc tggatatttt tcgcctaatt cttcagcaat
     1981 cgttctgact tcttttatag ctaataccgg atcgagccaa acatgtggat cgtattcatg
     2041 tgaatgacca tgcccttctt ctgattcttc atgttcgtct gcttcttgcg attctaaacg
     2101 gtcgattcct ttagctgctt cgatcatcaa ggtctgtttg ctatccactg aatcctttaa
     2161 cgaatccaca aaaaattcca tgtctgaact attatacacg aaaacatctg catcggaaat
     2221 ttctgctctt tcttttgcac ttggttcgaa atcatgcggc tccgttccag cagggatcaa
     2281 aagtttcacg tttccttcat cccctacgat ctcttttgta aaatcataca tcggataaaa
     2341 agtcgtaacg atcgtcaaat cctctttttt gtctgcttcc ttgttcgtat ttccacatgc
     2401 tgcaaacagt aaagcaccta tcattaccgt agctgtcaaa agatattttt tcatttatat
     2461 gctcctttca atgaacaaat cggaatttta cgatttatta acgtgaacta atgtatcaaa
     2521 ataaaaaaac acagtcaata gaaatccatc aatttctgat ttaagcatgt atattcttga
     2581 ttaaatcgta aaatggatga gttgctgctc atatctagcc atttcactac aataaaaaac
     2641 tgaagaatac cattttagga ttcctcagtt tttcatcacc tattttactt cttatatttt
     2701 attgcagggt tcgtactaaa taaacttcct cacaaaaacc ttttaaccca ttatatactc
     2761 tctgcgctct tgaataatgt tgacacaagg caaaaacggt tggtccgctt ccactcatca
     2821 atgcagcatc tgctccgtac ttgatcatac gctctttgat ttgatggatg actggatgcc
     2881 gctttcccgt tatatcttct aaactgtttc ccattttttt gatcatctgc tgataatctt
     2941 gctctcggat ggcttcagca agtccgtgga tgtcttgatg ttggatggct tgcgtatcta
     3001 cttctctaaa cactgtacgt gtagagacac tcattcttgg tttgaccaac acaacccagc
     3061 actgtggcat agaaggtaat acttggattt cttccccttt gccaccgaca aatgccgtac
     3121 ttccgtagac acagtaagga acatctgtac cgacttccaa accgatttct gccagttctt
     3181 ctaacgaaca gcccagattc cataattggt tgatccctct tagagcagct gcacagtctg
     3241 tacttcctcc gcctaatcca gctgctactg gaatattctt cttaacaaag atcttgacac
     3301 cggttttgat attgtattta ttttttagca ataaagcagc ttgataaaca ttgttccgtt
     3361 catctaccgg taaaaatgct ttatttgttt ctacaatgat tttgtcttct gttatttctt
     3421 caaaaaatag atgatctgcc aagtctacgc tcgacatcac catttccaac tcatgatacc
     3481 catcttgccg tctatataat acatccagtc ctaaatttat cttggctggc gctttttcaa
     3541 tcaattccat cctctcaccc attcgaaaag tctaagtcgc aagtattcta tcatagggaa
     3601 aaataaaagt atagcttatg tctgcctagt ggttgatttt gtcatcgttt caacagcttt
     3661 cacagaattt tctcttcata atccaaacaa gttctagtga ttcttataat tctgtgagca
     3721 cttcttctgc caaactcgga acatttcctt tttcttttcc tatcagactg agtagctgca
     3781 tggtagcaaa tccgaaatta cttcctttcc tataagcaaa ataacgtcct tcccaatgat
     3841 cgacatattt taatttcttt tcataagcag actggaagga atagaaaaaa ttaccataat
     3901 tatatacgat atgcatgact ttttcttgaa agaaagaaaa atctgtttct ccaacattag
     3961 ctaatggagc taagcctaga ttggcttccg agtagcccat tttctcgtat acaataaaca
     4021 gattggctaa cagatagtca gctaattcct cagattcttt aggtaagacc cgcaacaaat
     4081 catatcccgc ctgcttcttc gtcatcggct gagcggtgat gaagccaacg actttttgat
     4141 ctttacggac aataccgaca ccgctagata atacatatga tttatcaaat cgtcccacag
     4201 agaagtattt ttctcgtgca ccttgaagcc atttttgtga aacttcattt acagcttgat
     4261 gtaattcttc cggcagttga tcgtaccaag taaaatggta tccttctttc atcaaacgtt
     4321 gatattcaag ttgctgactc agccgagaca agtcttctgt tgctagatca acaatccctg
     4381 cttcacctac tttcgtgaag tgaaaaccca attcatgaag catcactacg tagctttctg
     4441 atatcttata aaacgcaagc tgatagccta aacaatctgc ttgtttcata aactgattcg
     4501 ttgctgcccg catcatttta ggattgccaa ctggatctcc taaaacaaag catttgtttc
     4561 ctttgatctg ataaccaaaa acgacttgat cttggtcgtc ttcttggtaa taaaaataac
     4621 tgtaacctga caagcgaagc tgatggcttg tagctgtgcc tccgtatgtt tccaataaac
     4681 ggtcaaaacg gtctttttcg tactcactgc caagcttcgt agtatcttca gctaaatatt
     4741 gataaagcgc cactaaagct agcaatgaga tgcccaaacc aataagccct gagaaccaca
     4801 catcatctga aggaaacaaa ataaaatgat ttcctgttat tcgaccgact aaaaagcgtc
     4861 cgctgtgata gcctgcaatt gcgtaaacga taaacaaaaa gccgaacaag ctagcatcaa
     4921 agattatagc tccccaagaa taaacgaatt ttgttcgata aaattctttt ctggataacc
     4981 acacagctaa cagaatcaag acatatacca atatcaactg ccaagataac gtacgtgtga
     5041 ttgtatttaa aataccaaaa gatagtaaag caatcgtagg cagaaatgcc tttttgactt
     5101 ttactgaaag tgctctagca aggccgagta ataaaaagcc aaccaataga ttcactgtct
     5161 gatctaaaaa attgaatgag aacggcaata atacttgaaa tagccgactg attgcagaca
     5221 gattagtgat tgttgataga agtaccatca tgatccccgc aaaatagaca gcacttacga
     5281 gcacgatatg cgcaaccttt tggaaaaaca gtcgcggaat gttatctaaa aatcgattga
     5341 ttttgactcc tgtatgtgtg atgaacaata gaagtcccgt cataaaaggt agaacataat
     5401 aaaacaaccg ataatataat agccaaacta ctgctacatc ttgccgaaca ggcaattgac
     5461 tcattcctaa gatcatcaaa acgtcaaaag tccccattcc acctgggacc atcgtcagca
     5521 ttcctatcaa tgtagcaatc atgaacatag gatagatcga aagcatcgaa atatctattc
     5581 tcatcaactt gccgatgatc aaaaataccg caagggctcc cgtccattgt ccaaaggatg
     5641 ccagtagcag accaagcaag ccttttggat aaaactcgaa gaacaatgtc tttctcttta
     5701 gataagcaaa aaagaaaatg actggtgcta aaaaactacc gccgaaaagc cagatccaat
     5761 agcttcgaaa aatactttct gactgaataa aaaagacatc aataaatgta agaaacgacc
     5821 atacagacaa accagtcagc ataaatagtg cgacttttga tacagtggct aaaaccattt
     5881 ttctatttgt ctgctttcca taaaaactag cccgcagact tgctcctacc acaccaccaa
     5941 aaccggcgag attgttgatc gtgtttgtta cccacgcagc aagaagaaac tcttttcttt
     6001 ccatttttgg ttttccttgt ttttctaaga ctctgatcgt cacccaatca tacaacaaca
     6061 tcggagtgac acccagcagt ccagccagaa tcatgagcag gatcgtcatg cggctttgtt
     6121 gtcccatcct ttgccaaata tcttgccaac tcatgccatt agcaatattt gcgacttgat
     6181 ttgccacaaa gactaacacg gagccgaaaa aaatcagttt taaaatcaga ctatgttcct
     6241 ttatccattg cagcatcaag tatcgtttat ttttcacatt ttcccattct taacttgtat
     6301 acttttgtgt cataccccaa tgttatctta tttattttaa tttttccact ttagacctgt
     6361 tctttgcttg cgatgataga aaaagaaaga tatcctaatg ctaaaggaga ggataacatg
     6421 aaagtaggat ttatcggcac agggaatatg gcacaagcta ttattgatgg tttattaaaa
     6481 aaaggaacag tctcgaaaga agaggtgttc gtttccagtg gacattttga aaatgcacaa
     6541 aaatttgctg ctgaaactgg cgtccatgca tgcaaaagta atcaggaagt tgcatcacaa
     6601 tcagatatcg ttcttttagc cgtgaaacca aaaatcattg aaaaagtatt ggaagaacta
     6661 agagaatata gccagaaagt cttattcatt tctattgctt caggaaaatc attaacagat
     6721 ttagaaacag cgcttgaaga tcctcaggcc tccatcgtac gtgtgatgcc gaatgtcaat
     6781 gtcagcgtag gagcaggcgt ttctgcgatt tgcaaaaatg aagctgccac ggaggaacag
     6841 cttcagcaag ctacgatgat ttttgaagca atcggcagcg tctatccttt agcagaatca
     6901 gatttcagta cattcattgg ccttgctggc agctcacccg cattcgttta catgctgatc
     6961 gatgcgatgg gacggactgg tgtactgaat ggtttgtcca aacagacagc cactgagatt
     7021 gctgcaaaag caattatggg ttcttgtcaa aaattccttt caagtgataa aaatccatgg
     7081 gaacttgtag accaagtttc ttctcctggc ggtacaactg ttgcaggtgt agttgcctta
     7141 gaagaagcag gattcatccc agcaatcatc aaaggaatca atacgacgat cgagaaagat
     7201 cttgagatgt tgaataaaaa ataatgatag caatgttttt tcgtgcctta caaaaaaaga
     7261 agagctgtgg cttttgccgc ggctcttctt ttgtattttt tattatttag cataatcagt
     7321 tgcacgagtt tcccgaataa cgattacttt gatgtgtcca ggataatcca gttcttcttc
     7381 gattcgttta cgaatatcac gtactaaacg aacagcgtct aaatccgaga tttcttctgg
     7441 cttgaccatt acccgaactt cgcgtccagc ttgaactgcg taacttgatt ctacaccggc
     7501 gaagctgttc gaaatatttt ccagacttct taaacgacgg atgtagtttt ctaatgattc
     7561 acttcgcgca ccaggtcttg ctgctgagag cgcatctgct gcagctacaa ggacagagat
     7621 aacagaagta gcttccacat ctccatgatg agaagcgatc gcgttgatta ctactgggtt
     7681 ttctttgtac ttggcagcta attccgcacc gatctcaacg tgagatcctt caatctcatg
     7741 gtcaagtgct ttcccaatat catggagcaa cccggcacgt tttgctaatt ggatatcttc
     7801 gccaagctct gctgctaaga taccagctaa ttttgcaacc tcgaccgaat gcttcaagac
     7861 gttttgtcca taactagttc tgaatcgcag acgtccgatg atcttgatta gatctggatg
     7921 aagtgtatga gcaccgactt caaacgctgc ctcttccccg tattcgcgaa tacgttcatc
     7981 catttcttta cgagatttct cgaccatttc ttcgatacgc gctggatgga ttcgtccatc
     8041 ttgtatcagt ttttccaagg tcatccgtgc gatttctctt cggattggat cgaatccgga
     8101 aagaacaact gcttccggtg tatcatcgat gattaaatcg ataccagtca gcgtctcaag
     8161 cgttcgaata ttacgacctt ctcgtccaat gatacgacct ttcatttcat cattcggaag
     8221 ggtaacgact gaaacagtcg tttctgaaac ttgatctgct gcgcagcgtt gaatcgctaa
     8281 tgataataag tttttcgctt tgcgatctgc ttcttctttg gcacgctgtt cagattcttt
     8341 taccattaga gttaactcat gattcagctc ttcttcagtc gatttcatga tgacatctcg
     8401 tgcgtcctct tttgataaac cagcaatttt ttctagttct ttttgttgat cttcaatcat
     8461 tttttcaacg tctttttctc gctcttcaat taattgctgt tttgaactaa gtgcttcttc
     8521 tttactttca agtgagttct cgcgtttttc taacgagtca tctttacgat tcagcgtttg
     8581 ttcgcgttga atcaaacgat tctcttgaga ttttaattct aacttacttt ctctcagctc
     8641 actttcaact tcagaacggt atttctgatt ttcttcctta gcttctaaca atgcttcttt
     8701 ttttagagtt tctgcttctt tttttgcact atcgatgatt ccagcagctg atgattgtgc
     8761 accagctatt tctttctcgt gacgtgactt tgcgaccata aaccctaaac caagaccgac
     8821 aattaaaccg atgatagcga ggagaatgtt gaataccata aaatccacct ccatactatc
     8881 tttttcattt ttatcatcta tacagcttct atgataaatt attctaaact cgtcaatatg
     8941 catcttcgca tatatgtgta caaaaaacat gcacaacttt attctaatgt ttgtaccccg
     9001 tagtgtcaac tttaaataaa caaagaaacg gctttctact tattcattaa acgatggttt
     9061 ttaaaataaa tgacactagt ttcatatagg taaaccttta aattgagaaa cttttctatt
     9121 aaattattct ttgtagatga acaga
//
LOCUS       DAGCYL010000112         8912 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.114, whole genome shotgun sequence.
ACCESSION   DAGCYL010000112 DAGCYL010000000
VERSION     DAGCYL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.114"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            29..847
                     /gene="purR"
                     /locus_tag="MB216_001980"
     CDS             29..847
                     /gene="purR"
                     /locus_tag="MB216_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBT4526779.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1350..2723
                     /gene="glmU"
                     /locus_tag="MB216_001981"
     CDS             1350..2723
                     /gene="glmU"
                     /locus_tag="MB216_001981"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBT4526780.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2817..3788
                     /locus_tag="MB216_001982"
     CDS             2817..3788
                     /locus_tag="MB216_001982"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBT4526781.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3794..3896
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3962..4531
                     /gene="thiT"
                     /locus_tag="MB216_001983"
     CDS             3962..4531
                     /gene="thiT"
                     /locus_tag="MB216_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBT4526782.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4612..5385)
                     /locus_tag="MB216_001984"
     CDS             complement(4612..5385)
                     /locus_tag="MB216_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526783.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5652..7352
                     /locus_tag="MB216_001985"
     CDS             5652..7352
                     /locus_tag="MB216_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBT4526784.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7322..7540
                     /locus_tag="MB216_001986"
     CDS             7322..7540
                     /locus_tag="MB216_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBT4526785.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7560..8450
                     /locus_tag="MB216_001987"
     CDS             7560..8450
                     /locus_tag="MB216_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBT4526786.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8468..8752
                     /locus_tag="MB216_001988"
     CDS             8468..8752
                     /locus_tag="MB216_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526787.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         3002 a         1528 c         1881 g         2501 t
ORIGIN      
        1 taaaatttct taaaaggagt tggaataggt gaaagtacgt cgcagtgaac gtctagttga
       61 catgacacag tatttattga atcacccgca tgaattgatt tccttaacaa gctttgccga
      121 acgttatgaa tctgctaaat cttctatcag tgaagatttg ggaatcatca agaaaacatt
      181 taaagaacga gggtatggaa ccttggaaac aatcccgggt gctgcaggtg gtgtattgtt
      241 catccctgaa atttcatatg aagaggcgaa aaaatatatt gaatctctag cagaacgtct
      301 atcagaacaa gaccgtctgc ttccaggagg atatgtttat ctctctgatt tgttaggaga
      361 acctgaactg ctgcgtcaag ttggaagaat cattgcttct aaataccttg gtgaaaaaat
      421 cgatgctgta atgactgtag ccacaaaagg cgtacctatt gcccaagctg tgtcctatta
      481 tctgaatgcg ccattcgtta ttgtacgccg ggactcaaaa ataacagaag gatcaacagt
      541 aagtgtcaac tatgtgtctg gatcttctga aagaattgaa aagatggaac tgtctaagag
      601 aagtttgaaa cgtggttcac gtgtccttgt agtggatgat ttcatgaaag gcggcggaac
      661 agtcaacgga atgaagagct tgattgaaga attcgaatca gaacttgttg gcatcactgt
      721 atttgccgaa tcaaaattca acggtcatcg cgccatcgat gattatacgt cactgcttta
      781 tgtcaaagat gtagatactc aaacgaaaaa tattacggtt gtgccaggaa attatttttc
      841 agaataagag gttgtgaaaa agctgcttag tttcgagtag taagaaaaat tttgtgaaaa
      901 tggctttcca cattttttca taaatttggc ttaataccga gaaactggct tttgaacacc
      961 gtttatctag aggttgtgaa atcagcgtcc tgtcctgagt attagctcaa taaacggaaa
     1021 aattgctcct catattttgc cgtttattga gctaataccg aaggtcagct gattgaaaaa
     1081 cgtttaatga agaggttgta agcctaccgt ttaactctaa gtcacaagaa acgatttcaa
     1141 aaaacagctt ttcatgtttt atgagtagtg tgacttgtga ccgaggagtt ggcaggagag
     1201 caccggttac caaatagata ggcccgttat ggtctatcta tttttttatc cagaaaacgc
     1261 tgggataaaa gaaaatcact tgttttgttg aaaaaatggg tatactggga taaactgaaa
     1321 tcagcaaact atatttagga gtgacgcagt tggacgcacg ttatgcaatt attttggcgg
     1381 cagggaaagg tacccgcatg aagtcaaaat tatataaagt tttacatccc gtttctggta
     1441 aacctatggt agaacatatc attaatcggg tcagtgaaac gaaaccagat gaagtgatta
     1501 cgatcgttgg acacggagca gaacaagtta aagcacaact aggagaacgc agtaaatatg
     1561 cgttacaagc agaacaattg ggaacaggac atgctgtttt gcaagctgct tctttcttgg
     1621 aaggaaaaaa aggaacaacg ttagttatca gtggcgatac accattattg actactgaaa
     1681 cgctgaacaa tctttttgaa tatcaccaag gaaaaaatgc aagtgcaacg atcttgacgg
     1741 cacaagcaga aaacccaaca ggatatggac gaatcatccg tgaccatatt gggatcgtag
     1801 aaaaaatcgt ggaacaaaaa gatgcaacac cagaagaagc tcttgtacaa gaaatcaata
     1861 caggaactta ctgttttgac aacgaagctt tgttcgatgc gttaagcaaa gttggaacaa
     1921 acaatgctca aggtgaatac tatctaacag atatcatcga aatcttaaaa gaagaaggcc
     1981 atacagttgc tgcttatcaa acggatgatt ttgaagaatc aatgggagtc aatgatcgga
     2041 tcgcgttagc taaagcaaat gagatcatga gaaagcgaat taaccagatg catatggtca
     2101 atggcgtaag ctttgtcgat tcagcaacga catacatcga tgcaggcgta gaaatcggtc
     2161 cagacacatt gatcgaagca ggtgtacaaa tccaaggaaa tacagtcatt ggttctgatt
     2221 gtgtcatcgg gtcacattca aaaatcgtag acagtaggat tgaggatcac gtcgtcattg
     2281 aaaactcagt cattgaaagc agccatgtca agaaacatgc agatgtggga ccttatgcac
     2341 atttacgacc aaaagctgaa attggcgaaa acgtccatat cggaaacttt gtggaagtga
     2401 aaaatgctca aatcggcaaa ggaactaaag tcggtcactt gacttatgtt ggtgatgcta
     2461 cgctgggaga agaaatcaat gtcggatgtg gtgttgtctt cgtcaactat gatggaaaga
     2521 acaaacatca tacgactgtc ggcgaccaca gctttattgg ttctagcacg aatatcatcg
     2581 gacctgtcga ggtagctaaa aacagttcga ttgctgccgg gtcaacaatt acagacaata
     2641 ttccagaata tgcgctggca attgcgagag caagacaagt caataaagaa ggatatgcaa
     2701 aaaaactccc ttatctaaac taagactttt gcaaaatcac ttgatttatt taaaaaatat
     2761 gactactatt tattaggtag aattctagag ttgtgaaaaa gaaagtggag gtccccatgt
     2821 caaaacatta ctttgaccca agattaaaaa tttttgcgtt aaattccaat cgcccattag
     2881 cagagaaaat tgcagcagca gtcggcgtcg agttaggaaa atcatctgtt actcaattta
     2941 gtgatggaga aattcaagta aacattgaag aaagtattcg tggatcgcat gtctatgtta
     3001 ttcaatcaac gagcagccca gtcaatgata acttgatgga gttgctgatc atgattgacg
     3061 cactgaaacg agcaagtgca aaaacaatca acgtagtcat gccttattat ggctatgcgc
     3121 gtcaagaccg taaagcacgt gcacgggaac caatcacagc gaaattagta gcaaacatga
     3181 ttcaaaaagc tggtgcaaca agaatgctga ctttggacct tcatgcagtt caaatccaag
     3241 gtttctttga tattccagtc gatcatttga tgggtgcgcc acttattgcc aattatttct
     3301 tagaaaaagg aatcaaaggc gatgatgttg tcgttgtttc accagaccat ggtggtgtca
     3361 cacgggcacg taaattagcg gaatttttga aatctccgat tgcgatcata gacaaacgcc
     3421 gtccaaaagc aaatgttgca gaagtaatga atatcattgg acaagtcaaa ggcaaaactt
     3481 gtgtactgat cgatgatatg atcgatacag caggtacgat cacattggct gccaatgctt
     3541 tgaaagaagc tggtgcaact agtgtgtatg cttcatgtac acatccagta ttatccggcc
     3601 ctgctttaca aagaatagaa gattcagcta tcgagcgtct agttgttaca gactcgatct
     3661 atcttccaga agatcgtaaa atcgacaaaa tcgatgaagt aagtgttggc ggactaatgg
     3721 gagatgcaat caaacgtatc catgaaaaca aaccagttag tccattgttt gaaacaaaaa
     3781 ataaataata aataaactca caaaggggag tccaattggg ctgagattga atctatttct
     3841 aaacccttcg tacctgtatc ggttatgcga gcgtaggaat tgtgaataaa cagctgtttg
     3901 actattttcg aaagctgctt cctcctttgt gactttttta ttcaaaggag gaagtttttt
     3961 tatgggaaaa aatagagttt gggtagaagg aacgattgtt gcagcgttag cgatggtgtt
     4021 gtcgttgatt ccgatccaga tcggcagcag tttttctatt tcattagggc agattccatt
     4081 gactcttttt gcgttaagaa gagggacaaa ggcagggatg ctggcaggtt tcatttgggg
     4141 catcctacat tttccgttgg gacaagttta ttatttaagt gtcgttcaag tgttgattga
     4201 gtacccgatt gcctatactt ttgcaggcgc agcagggctc atggcttcaa atgtccaaaa
     4261 aggctatgta gacgataata cgaaacaaat tagaaaaagc atcgtgtttg gtgcattgat
     4321 tggcgcactt tccagatatt tttggcattt tattgcagga gtggttttct ggggagcgta
     4381 tgcactatgg gggatgaatc cttggctctt ttctttcgta atgaatggtg ccagcgggtt
     4441 gagtacagct attgtaacga tcatcgtttt attagggatc caacaaacga ctccttcgat
     4501 ttttgtgcca gataaacgac aggagctgta aataaaaaag aagcaagaaa cttgagacag
     4561 aatcatgtcc tagtttcttg tttccttttt attttttgat aagaaataga tctacttcaa
     4621 tggcagataa gaaagatcgg catcttctac caattgccag tgtcgatcgg tgacagagta
     4681 gattaaagta gcgatgattt cctcttctcg ataccgggtg ccttttaatt tccaaatata
     4741 atcttcaatc acatagattg ggatacgata tttaccatcg atcaaaccaa cttgatcaaa
     4801 agaaaaaatg atcccttcct gcatcaaatt acgcatttct ttcaataatt ccacactgat
     4861 tttttcaaca ggtgtttctt ttttgaaaaa agattttttt acattgatct tatctgacaa
     4921 gatcgaactt aatttactag caaaaatcct ataaaattca tctaaatagc gataataaat
     4981 ccgattcagc tgatcattaa gatcgaagta tacaaaatta ttctgtagtt tataaaaaaa
     5041 cggcgaatga agatgtgttt tcatatgacc aaaatataat aattctgaaa tttctaaagg
     5101 tgtcaattct ttcagcatcg ttaaatcagt aaagtcgatc cacttcaatt catcgaatcc
     5161 acgcctgtta cgagaagagg aaaagaaacg ctgaatattt tctgtaccac ggattacttt
     5221 cagccctgta tgggattcat attctccagc agaagcagat ggatcaagca agagcaagtt
     5281 ttgaggaaaa tgcacgatac tgtttgcaaa gtcagtagca gtcagccctt ttgttaaaac
     5341 agcattactt gtggtatcaa tatgtacata aactgactca gtcatcatgt tcctcctctt
     5401 atttcctctt aatctctttt attttacaac atttgcatta aaaaagcttg tttcttttat
     5461 tttaaaattc cattacaaaa tcacgttttc attgaaaaca ctctcatttt caattaattt
     5521 ttgtgatttt tgtatttttt tatttaaagg aggttttatg aattgttttc ttatggaatc
     5581 tacaaagtcg aatacgagac tcatgacctt tttatgacgt actggtatac ttgaagaaaa
     5641 aggagagcgc gatggagata aaaaaagttt acttttcaca gaaaatgtcg ctgtattcgc
     5701 ctatccagca agcaaaacaa ccccttccat tttttcaaag tcatcaagaa agtagagacg
     5761 ctttttggcg ttctgcaaaa aagcaatgtg cttttaaagc agtatcgaaa aaatataccg
     5821 tctattttac tattgctaaa agtagaggag atgttatttt taataatctg ttgattgaag
     5881 gtgaaacttt tgaatggaaa aaattttttc ggtatatgga agcttgcgct cgttttttta
     5941 ttaaagaaaa ctgttgtttt ttatttcaat ctcctctatc agaagaatgg ttgcgtattt
     6001 ttcataaaaa cggctatcaa ggaaccacac aattaaataa aaagctggtc tatcatacag
     6061 cacttgtttt aggtggagga ggagcgcacg gtgcttatca gattggtgtg tggcaggcgc
     6121 tgaaggaaca tgatatcaac tttgagatta tcacaggtac ctcggtaggt gcgttgaacg
     6181 gcgcattgat tttacaagga gatatgaaaa aagctgtgaa tttatggaaa aagctgtcga
     6241 caagacaagt gcttgcgtta ccagaaatgg ctgcagttga agaccttcgt gaacgcttct
     6301 atcgagaaag acgccaaatg acgaaaacag cactaattga aggcggggtt tcttctgctc
     6361 cattggaaaa gatggtcaaa gacaatttgg atttgtcctt actcaaacat aatcccaaaa
     6421 tacgtttata tacggtgtct actaaactgc cagaactagc ggaagtagtg actgatatcc
     6481 aacagacaaa aacagaagaa ataccagatt ggatactcgc ctctgcttca ttttatcccg
     6541 ctatggctta tcgaaagatc ggaaaaaata aatatgcaga cggtgggtat cgcaacaaaa
     6601 tacctattga tattgcgatt aacaaaggag caacagaggc atttgtcgta gatgtgcaag
     6661 ggccaggccc agcgaaaaaa attcgtatgc cagacatttt tatccattgg aaatgtcaaa
     6721 cattgtggac actaggcagc tttctgcttt ttgatagcca aaggaaccaa ttaaatttac
     6781 aattaggcta tctggaaatg aaaaaacgat taggctatta ctatgggaac tggtatacat
     6841 ttcattctgt taaacctgca agaacatact ggcgaggatt tttgtcttac ttaacaaaag
     6901 aaattcagtt agaattatct tttttcaaaa gcataaaatt ttggcaaaaa ctgcgtaatt
     6961 tatataagaa cagggtcgta ccagagacat gcggattggc gatgctagaa cttttagcaa
     7021 aaaaacattt tttactgccc aatgaaatct atcaagtaga catgatgatt caaatgatct
     7081 gccaacaaga tataaaaagt atgccggatg atttattggc acaaattggt caattaagta
     7141 ctgaagaatg gcgaaggtat cgaaaatacc agcaaaaaat acaaaatgag cgcacgaaaa
     7201 ctgtttattt tgattatctg ctacaaggaa aacgcaagga tcgtttgcag catgaattgc
     7261 ttaagcaacc agttgacaca ttactgatcc tatatttata ttatctcaag gaggaacaac
     7321 catggcacaa gaattttcat atgaaatcgt agaagaaatc gctatattgt cagaaaacaa
     7381 caaggggtgg cgtaaagaac tgaacctagt cagctggaat ggtcgcccgc caaaattcga
     7441 tcttcgagat tgggcgccag accatgaaaa aatggggaaa ggcctgacat tgacgaacga
     7501 agagtttgaa caactgcaaa aagcaataga aaatatgtaa tgaggaagga ccctgtcgaa
     7561 tgaaaagtat gactggtttt ggcaaagcca tccgcgaaac aaaagcgtat cagttagata
     7621 ttgaaataaa aagtgtcaac cagcgttttc tagatgttca aatacgaagt cctaaaatcc
     7681 tgaactttgt tgaaaacgat tttcgtcagc ttattaaaca gcagttaagc cgcggtcgaa
     7741 tcgaagtatt tgtgaatcta gcttatattg gcaaaaacga aaaacagatc gtcatcaact
     7801 gggaattgat tgattcactt atcactcaat tgacagacgg aattcaacaa cgatacggta
     7861 gtcaaaatca agtgtcaatt agtcggatgg cggaacgctt cgcgctggac gaaaacttcg
     7921 taacggtaga agaaaaaccg gaagagagca tggaagaatt ggcacagtta gctctagata
     7981 cattgaaaag cgcacttgat tctattgagg cgagtcgaga aaaagaaggt caagctttag
     8041 ctttagtgtt gaaaaagaat actgaagaat tcaaagaagt actaaaaaaa ctcgatagtt
     8101 ttgtagaaat ctatgaaaag gaatttcaaa taaggtatca aaaaaagctg gaggaatttc
     8161 ttggagcgaa agtcgatgag caacggttgt tgacggagct agccatcttg ttggaacgcg
     8221 gagatattca cgaggaactg gatcgtctgg cgatccatgt acaaaaacta gatgaactta
     8281 tcgaagcaca atgtcctgta ggtcgggaat tagatttcct gatccaagaa atgaatcggg
     8341 aaatcaacac gatcggttcc aaatcaaatg caatcgaaat taaaaatcaa gtcgtccagt
     8401 ctaagacgat cttagaaaaa atcagagaac aagtacaaaa tattgaatga cgaaagaggt
     8461 agagataatg aaatattatg acgtaacgtt ccacgaatta agtggaaaaa ctgtggttaa
     8521 acgggatatc ccttctgaaa aaaacggctt tgacgtatgg aaagatgcct gtgcagacta
     8581 taatgagaac gaactgttta ttttgatcaa cgacggtgct tacgtgacga tgaatcggaa
     8641 atttattgtt cgaattgaca cagaagaggt agaagatccg acagaaaaag cacgttctcg
     8701 aaaagatgag atcatgggtg tagtgaatac tttgtccaat atgggctttt agcaaggatg
     8761 ctgaacaaag aaatacaata aataatcaga attggatttc ttcgcttagc ttctttgctc
     8821 taggaataca tttaaaggtt gtgacaaaat tcgtgtcaca atcttttttt attttgaata
     8881 agtgatgttc aaaaaccatt ttctcggtat ta
//
LOCUS       DAGCYL010000113         8877 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.115, whole genome shotgun sequence.
ACCESSION   DAGCYL010000113 DAGCYL010000000
VERSION     DAGCYL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.115"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..684
                     /locus_tag="MB216_001989"
     CDS             <1..684
                     /locus_tag="MB216_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HBT4526788.1"
                     /translation="GNEIVSKMKISEVTLDETDDFTYYKGYKKGWNTLCTYLKIRVPF
                     DDLDFFESHKDLITQTASSIYDKQGDNVLVDTILVPLPENYEVINFSQLKISDVVSQA
                     IEDAESFMSNGEYQRAFDRVHTAFHGYLIEILKKYEITVPRDENLSKLYSRIQQLIEK
                     EIQPTELADIVKTTIRSSNGMISSLNEARNRHSLAHPNTNIIGKREAKLIIGISSTVT
                     DYISGYLDK"
     gene            751..1245
                     /locus_tag="MB216_001990"
     CDS             751..1245
                     /locus_tag="MB216_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBT4526789.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            1301..1939
                     /locus_tag="MB216_001991"
     CDS             1301..1939
                     /locus_tag="MB216_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526790.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            2011..2406
                     /locus_tag="MB216_001992"
     CDS             2011..2406
                     /locus_tag="MB216_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4526791.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            2390..4843
                     /locus_tag="MB216_001993"
     CDS             2390..4843
                     /locus_tag="MB216_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4526792.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4840..6867
                     /locus_tag="MB216_001994"
     CDS             4840..6867
                     /locus_tag="MB216_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526793.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6864..7886
                     /locus_tag="MB216_001995"
     CDS             6864..7886
                     /locus_tag="MB216_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBT4526794.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7903..8832
                     /locus_tag="MB216_001996"
     CDS             7903..8832
                     /locus_tag="MB216_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4526795.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
BASE COUNT         3019 a         1492 c         1772 g         2594 t
ORIGIN      
        1 ggtaacgaga ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat
       61 tttacttatt ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata
      121 agagttcctt ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca
      181 gcaagctcca tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca
      241 cttccagaga attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca
      301 caagctattg aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac
      361 agagttcaca ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca
      421 gttcctagag acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa
      481 gaaattcaac ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga
      541 atgattagct ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat
      601 attattggaa agagagaagc aaaactaata ataggtattt caagtacagt tacagattat
      661 attagcggat accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt
      721 tttataaaaa ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg
      781 tataatgaag gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg
      841 gcagaaagaa tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt
      901 ccttttgatg tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt
      961 caagaaatcg aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt
     1021 agtagcttgg aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat
     1081 tcgatggaag atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct
     1141 tccaatttac aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt
     1201 aattacctag ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg
     1261 ttataattaa attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa
     1321 tcttaaacta ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt
     1381 tcgttacaaa cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt
     1441 tccagaatat gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct
     1501 agctgtctat gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt
     1561 tggtatcgaa tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc
     1621 tcgatttatt ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg
     1681 tttctcatta agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag
     1741 aagaaatcct ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa
     1801 aacgagattg tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt
     1861 cagatattca atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac
     1921 aaaaaataaa attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt
     1981 tttctatttt tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt
     2041 tggtcagtag aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc
     2101 tttacgcaaa tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta
     2161 ccaccacttt ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc
     2221 actgggttca tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct
     2281 atcattacct atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa
     2341 aaagaaacaa tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc
     2401 gattaaatat atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta
     2461 tgaattaatt ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga
     2521 taatttctgt caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc
     2581 aaccgaatca agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt
     2641 aaaagagata gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg
     2701 tgacaatcaa gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga
     2761 agtatccgta aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg
     2821 tgtgaatcac aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt
     2881 ttctaagatg gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt
     2941 ggagaaagat gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt
     3001 tgatgactac gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa
     3061 atacgatatt ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat
     3121 tgaacaggaa gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga
     3181 attggatttc cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt
     3241 tgataccagt atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa
     3301 taaaaagaaa gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag
     3361 tactaatgtg gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac
     3421 caaagaatct atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga
     3481 gctgaaacaa cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt
     3541 acgacctttt ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata
     3601 tatcaatgac tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc
     3661 tacacaaatg attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag
     3721 aaatatttat ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc
     3781 attagcgtcc gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct
     3841 ggtctattat tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg
     3901 tggtgaatgg aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac
     3961 gagtgagaac gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga
     4021 ttcagaaagc cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag
     4081 cgagaagttt cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg
     4141 gttgttattg gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc
     4201 tgaccatatc gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag
     4261 tgtcacccaa tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt
     4321 cttacccgat tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc
     4381 aatgctgatt gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt
     4441 taaaattgtt gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct
     4501 atctatgcga ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca
     4561 aaacgcggac gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt
     4621 tcgttccact gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga
     4681 agatgaaaac aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga
     4741 catctatggt cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc
     4801 ctttgatacc agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa
     4861 tttcttcact atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc
     4921 acgctgggca atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat
     4981 gaatactcca agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg
     5041 ggctggcttc catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat
     5101 gccattacta attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg
     5161 gtgaaagaag cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac
     5221 atgcagacga ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt
     5281 ttcctccttc tttttattct cattgttgga atctatgttg cctatacagg attggttaag
     5341 cgagaaacga cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg
     5401 gcttccttta ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat
     5461 gtcagtaccg ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa
     5521 gggaaagaca gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca
     5581 tggctattgc ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa
     5641 agtttattgt ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt
     5701 gaagaaattg aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta
     5761 ggaacagtct tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta
     5821 acaggaatca tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc
     5881 agtttcctat taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag
     5941 ttatttaatg tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt
     6001 atttccacta tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta
     6061 caaattgtga cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact
     6121 ttacaaagca acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg
     6181 atgaatcgac aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca
     6241 gggtcaacga agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat
     6301 actcgtcaaa atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag
     6361 caacaaggcg gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa
     6421 aatcgtctag tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat
     6481 gtcaaatatg cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa
     6541 caatctatgg cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa
     6601 aaacgaaaga acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga
     6661 aaagaaaacc catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa
     6721 accgtcacgc ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa
     6781 aaagagcaaa aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga
     6841 acggataagc gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct
     6901 tctctctctt cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca
     6961 gtggcgaaaa caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag
     7021 tgttggcaca ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg
     7081 tttcttatat cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc
     7141 aaagttccga atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac
     7201 aaggggttaa atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag
     7261 attctgttat ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg
     7321 ggaaaaaata tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa
     7381 aagcagacta tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg
     7441 gaaatcagtt ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat
     7501 ttgatgatga aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct
     7561 atgtctttgg aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg
     7621 tgtatgacaa agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc
     7681 aagaaatctc tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata
     7741 atgcaggcac atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg
     7801 caggcgaccc aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg
     7861 gggctagacg tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag
     7921 cgaaaagaaa agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt
     7981 ccgatgatta aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt
     8041 ggtagtgtcg gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt
     8101 gaaacagaaa tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa
     8161 tcctttgcga aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat
     8221 gaaaaattga cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt
     8281 aaggatattc ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg
     8341 aatgagaaca cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac
     8401 aaggaaacta tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg
     8461 gttattatca aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa
     8521 cagctagaaa gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta
     8581 gaaacgttct tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt
     8641 aatcatgtgt tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc
     8701 tttaccagaa aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa
     8761 acaaaggcaa cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag
     8821 attgtaaaat aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactct
//
LOCUS       DAGCYL010000114         8832 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.116, whole genome shotgun sequence.
ACCESSION   DAGCYL010000114 DAGCYL010000000
VERSION     DAGCYL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.116"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..831
                     /locus_tag="MB216_001997"
     CDS             <1..831
                     /locus_tag="MB216_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBT4526796.1"
                     /translation="FEIKNLVEISGMSKNTILSALDELQKVKLLVKRKNGFNKSKMQM
                     FPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGTNQYSVDNSKFELCDTS
                     MKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLSTKNNKLDEELIETFIE
                     ENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNN
                     KYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ
                     "
     gene            907..1125
                     /locus_tag="MB216_001998"
     CDS             907..1125
                     /locus_tag="MB216_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526797.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            1112..3790
                     /locus_tag="MB216_001999"
     CDS             1112..3790
                     /locus_tag="MB216_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526798.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSETVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3781..3978
                     /locus_tag="MB216_002000"
     CDS             3781..3978
                     /locus_tag="MB216_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526799.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3982..4593
                     /gene="mobC"
                     /locus_tag="MB216_002001"
     CDS             3982..4593
                     /gene="mobC"
                     /locus_tag="MB216_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBT4526800.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4578..5969
                     /locus_tag="MB216_002002"
     CDS             4578..5969
                     /locus_tag="MB216_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBT4526801.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            6185..6472
                     /locus_tag="MB216_002003"
     CDS             6185..6472
                     /locus_tag="MB216_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526802.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNK"
     gene            6492..6986
                     /locus_tag="MB216_002004"
     CDS             6492..6986
                     /locus_tag="MB216_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526803.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            7054..7368
                     /locus_tag="MB216_002005"
     CDS             7054..7368
                     /locus_tag="MB216_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526804.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            7467..7712
                     /locus_tag="MB216_002006"
     CDS             7467..7712
                     /locus_tag="MB216_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBT4526805.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMDRLQLTRQKN"
     gene            7753..>8832
                     /locus_tag="MB216_002007"
     CDS             join(7753..8029,8029..>8832)
                     /locus_tag="MB216_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBT4526806.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYET"
BASE COUNT         3396 a         1229 c         1687 g         2520 t
ORIGIN      
        1 tttgaaatca aaaatctcgt agaaatttcg ggaatgtcaa aaaatacaat cttatcagca
       61 ctagatgaac ttcaaaaagt taaacttctt gtgaaaagaa aaaatggctt caataaaagt
      121 aaaatgcaaa tgttcccaaa tttttattat ttgttaaagc ctgttctgtg caaagatgat
      181 gtttataaaa ttaataagat aacccatcaa gaaaatagac aaaaaaactt gatagttcaa
      241 aatttgaact atggaacaaa tcaatattct gttgataatt caaaatttga actatgcgac
      301 acatcaatga aaacaagaga caaaaacaat tttgctgata gttcaaaaaa tgaactagaa
      361 ttatataagt cttataaaga tagtaaagat attaaagaat cacataatga tgatcttctt
      421 gaatcacttt caactaaaaa taataaatta gatgaagaat tgatcgaaac atttattgaa
      481 gaaaatcttc ttaaacaaat atggggagaa agtattgtga attgcttaaa actggctagc
      541 aatagtgatt atcgtcaatt cgacaactgg tacaaaaaat tcaaatatgc gattagatct
      601 gctgaaaaag aacaaaaaat tcaactgaaa attatctatg aaatttgtaa taacaagtat
      661 tttgtagatc atgttagaga acagctatca atgactttac gtgacttgat acgaagagca
      721 aaaacagatc atcgtatcaa acaaaaagac gattatatct ttgcttctct aaaaaataaa
      781 gcgttagaac taattgaatt gcaaatcagc gaaggaatag ataagcaata agaatagaat
      841 tgagtcaaat gaaatggagg ggtatctcca cttcatttga gaactttgaa tataggaggg
      901 gttattttga ttaaaaatcg tttaatagat caagtttggt gtctagatca aaatacagat
      961 agtctcgaat taatcgagac tatttgcttc aacactatag ttctcgattt aagagctgaa
     1021 aatgataatt gtgtaactca tgtcataatt aatcaaaaaa tggctcagaa gctctctgag
     1081 agcctcagaa aatgggctaa gggaggtaac gatgaaaata attgaaaaaa agattgccaa
     1141 taaaaaaata aattggttgg ttgttaattt tcttgttctg atagctatga ttatttttac
     1201 aagtaatatt gctcatgcga gtattttcaa ttggtttgat gacacaaaaa gtcaaacaga
     1261 gtttttgaat aattctcttt atagacctta cctaaaaaag tacaatggag atttccttag
     1321 tcagttcggg atttggctcg gatgggctgt ggtaaaaggc atgtttactg tgacagattc
     1381 gattcaaaat atgattccag atgtgctgga tttgttcaat ttcattgaaa gtacagggct
     1441 aaataatgtt tatcaatcag ccgtaaatac aattgtcatt ggattaatga tcctttcatt
     1501 gatgtttgtt ggttacaaaa tgattactgg aaaaggaaca gtggatttaa aatcggttgg
     1561 aatgaacgta gtgatgtcta tagcattaat acttttaatg ccaaccatga tttctagtgg
     1621 tattcaattt gctaaaacat tttataagga ctctactacg attacaaatt cagatgacgg
     1681 agtcgcttgg tcattgataa aacaaggagt aacagattta gcatatatta ataaaacaga
     1741 tcagtactca agcattgata aaacggagga tcgaaataat ttaacacgaa aaaattttca
     1801 acagacggat ctcacacaag tgttaacgga taaagtgatt gataaattgg aaaaagaaaa
     1861 tccaattgcg gacaatcttc gttatgaatt agttgaaaat tctaataatg aatttgttgc
     1921 tacaaagttt tctgacaact tcttatcaac attttctgat agcttgaaat caggctatta
     1981 ccgataccaa gcaaatctat gggggatttc aattgggttg acagcgttag caatcgccta
     2041 tgtgttcagc gcgtttgtga tcattacagc aatactagaa cttgcattta aacgtgtgtt
     2101 aggtgttctt gtttttgcaa cagatattga gacaggacag cgttcaaaag ttgttctatc
     2161 agatattcta caatgttact taactgttgg gtttcaagga tttgggttat caatgtttgc
     2221 tatgtttatc aattttctga actcaggaca aggtatctca acaaatattt ttataaaaac
     2281 aattgcttat atctgtgccg tttttgtttt aatcaaaggc agtgaaacag tcatgagata
     2341 ctttggtgtt gatattggac tcaaagaagg ctatgggcaa cttgcttctg cttttggaat
     2401 gggcgcaatg ctgtttagaa aaggatcaaa tggatttaat cgtgctaaag gaagcggaga
     2461 aggagaagat cgaaaaccag aaaagaattt cggcgaaact ctatcaaaaa aagctggcaa
     2521 aactggaaga gctttgggat atgcccatga acgtggatta tccggtttag cttcagatgg
     2581 agctacaatg gctagtgaaa gagcaacaaa accatttaag agtatgcgtg atatagctaa
     2641 tgataccaag aataagttta aagaaggttt agatgatggt acagtgtccg ctattaataa
     2701 aaatagcaaa cctatgttag ctaaaaataa agaagatgaa actggaaaat atgctgattc
     2761 cgtgccaaca cgtctttcag atagaaaaga tggtgctaaa gttgaaaatg ccgatagaat
     2821 tatgtctagt tctgaacgta tgcgtgaagc aatgaaaaat aatgctgaat cgaataataa
     2881 tcctgcaagc gcaattcagc aaaaagtcca acaagatatt gaagaacgta aaaatgctat
     2941 gcacggtcaa gctaagtctg ctgaagaatt aattaatcag aagcgtcatg aagcgaaata
     3001 cacgcctgaa gcaatgaatc gcgaggaaat aatgttaagg aaacgtgtag aagggcggac
     3061 aggcgctaat attgacggaa aagaagcgtt gaccaaagaa agaattcaag aagctaaaaa
     3121 ttcaaataca ggattagaaa aattagtcaa agaaaatcca caaaattcta caagtagtca
     3181 tggtggccga tcagtagatg tccgagaaaa tattcaaggt tcactgaatg gtcatgttgg
     3241 acggaatgtt gatgtaagag agaatttaca aagttcttca aatgctcaag gtactaagac
     3301 aattgatgtt cgagaaaatc tgcaaagtag cagtacaagt caaccaaaaa cagtagatgt
     3361 tcgagaaaat gtacaaaatt ctacaagtag tcaagggttt aaatcagtgg atgtccgaga
     3421 gaatgtgcaa ggtagcagtg caagtcaacc aaaaacagta gatgtccgag aaaatgtaca
     3481 aagttctaca agtagtcaag gattcaaaac agtagatgtt cgggagaaca ttcaaaaaga
     3541 tagtggagaa atgaaagaaa aaacacaaaa aatcaatatc gttgaagaca gaaagtcagc
     3601 gaaaaaattt gattcaaatc atgaaacagt gattattgat agtgaaatcc gtgaaaatga
     3661 taagggatca aaatcaagac gaagattcac ttatgaagac aatgagttat tcagagatac
     3721 acttaatgat cctaatccac ttttcgataa tttattaaga aataaaaggg ggggattgtc
     3781 atgcaattaa ttctaaagat tagtacgcat gatttcttag aacttgacga attatgtgaa
     3841 tcagtaaaga acgcaacaaa tgaaaaaatc acggtcacta attatattga actgatgatt
     3901 caagaagaat taaagatgaa aaatgaaata gagagcaatt tcaaatgttg ctctctagaa
     3961 aaaaatgtga ggggataatc aatggatgat aaacaaaaaa tgtacgaaaa aaatcgcaaa
     4021 catagtgagg ccttaaattt tcgtgtcgat aaaaagactt atcttgaact gacattgatt
     4081 gcaaaacaaa ggggaatttc tagagcagac gtttgccgtg agcggttaaa gggactcaaa
     4141 tatgaaccgc cgttgttagc tgaagatgat agggtagttt taaccaaaca gttaaatcaa
     4201 attggtaaca atttgaatca agcggtaaag gcattaaata tcattgctaa atattttaga
     4261 gaagaatctg aagataaaaa agaaaagagc ctacatttta gttttgatgc tagccgttac
     4321 aacattttta aagatgaact tgcgaatagc atagcaaaaa agttgatgat tagttatcca
     4381 gatgacattt ttttggagtc caatcggagt gccccctctt tttttaaatt atcagaagta
     4441 gaaaaagaat atttcgaata ttggatagaa aagcaaacta gaccgggtac gaaagaagag
     4501 tattcccaaa aagttgatcg gaatttaatt cactcaattc agattattgc aagtttaaaa
     4561 gatgaggtga aacaactttg ggagctacta taacatcgaa aggacgcaag aaaaatttat
     4621 cctcgctatt acaatatgca gctaatcctt caaaaaaaga aaaatgtgtt gcagtctcag
     4681 gaataaattg tagtgatgat agtgaggaag cactgcaaga aatgaattta gtacgtacca
     4741 agatgaataa atctaaagat atagcacaag cgtttcatgt tattcattcg tttgataaga
     4801 gtacttctaa agagttaagt attgataaaa tgcacgaaat agctgtcgaa tttgcagaga
     4861 aagcttttcc aaattcgcaa atcattgttg caagtcacaa tgataaagat cattttcata
     4921 gtcatcttgt cattaataat attaacatgg aaacatgtaa aaggttgcgt attgacccta
     4981 aagatttaga actaatgcga aaaattaacg atcaagtcct caatgaacat ggtcttgaat
     5041 cagtgaataa tgagtactat gaaaacttag aaaagagaac caatctttat tcaaacgatc
     5101 cttctaagat gaaatatgga aaaattaatt atcaagagtt tgtgcaaaat gcgatcgaag
     5161 caacgttgga aaaatgcgaa gtgaattctt ttgaagattt ttcggatcat ttggcaaaag
     5221 aatataacat tgaagtttat caattttcaa aaacaaataa caaacttggc tatgttcttt
     5281 acaaacaagg tgtaaatttc tatgagcgta gaggtgagtt aattacttta ggaagagata
     5341 aacaatctag aaaagaaaaa agtcagtatg tcgaacgtac ttttagtgca agaaagttag
     5401 ggaaaagata ttcatttgaa tctgtttctg aacaacttga aaagaattta gaagaatcac
     5461 atataaaaaa tttttttaat atggaaagag agcaagttat gtcgctaatt agtggagtaa
     5521 attacaaaac tggtgagtta aatgtaccgt ttgtggaatt agattccatc gcacaaaagc
     5581 aatttagcga ccgtgaaggc gatgaaaaat tcattaaata tgatgacttc ttgcgagagg
     5641 tagacaagca ttttgtaaat acaatgtctt tagaagatga tcaagaatac acgattaaac
     5701 ttcatacgga tgtgaatcct caaactttac gtagtgcgaa aagtaaaaca atttctatta
     5761 aaaagactga tcagaaagag ttatttctac cggcaaaggg ttttgaaaat tgttctcata
     5821 caccttctgc taaacagatt cactatcaaa tcagtcgcat aatgaatgac gatgtaaaaa
     5881 tgtatcgtgc taaaaaggat tatgaattca cacaacaaat gattgaaaat cagcaaaaaa
     5941 ggcaacaaaa ttttgaaatg gaattctgaa tgggcttgtt agtggacttt ccagtttttt
     6001 gcctatgaag agttgcttgg aaagaagcag tttttaggac atacgccagc tacccatcta
     6061 cttgccagtc aatccgcccc gctcacgcta ggctactttc tggcaagtca gatggtgggt
     6121 aaatctctga gggagcaagc tccctacaga ctccctccca aaaaaagaaa ggtggaagaa
     6181 aaaaatgaac tatccatatt ttaaagtctc ggcttcagaa gaaactaaag aaatattcaa
     6241 caatttttac aaccaaaaca aaggagtttt tggttcaaag gcaaatatgt ttcgtgttat
     6301 ggtttcgaat ttaccagtgt tagctagtcc atcaaataat aaatttaatg atcctgaaag
     6361 cattaaattt gaacaaaaaa tttcagaact agaatcaatg ataagtaatg aagtaattga
     6421 aaagctggat gacattgatc aaaagttatc gtattctcta aaaaacaaat aaaaaacgga
     6481 ggagaaaaaa gatgtttaaa gtttacttcc cgtttcagtg gctcgttaaa ggaattcttt
     6541 tagtgctact aggattgatt ctttttggaa gttatctaac aattaaagcc agacattttc
     6601 cagaagaaaa gccaataaaa attgtaacaa aagatttaaa aataggtgaa acttatcagg
     6661 cagatggggt tgaactaaca tttaaaaata ttgaagtgac cgaaaaaaga aatgaatttg
     6721 cacaacatga tcgtgtatta caagtgacct atacacttat aaatgataca gaaaatccga
     6781 tacgtccaca acaatggtcg attgaaaata aggatggtga agaactttat gagtatgata
     6841 tgatattgga taatccagag tatattgaaa gtggcaaaga agaaacagta aattactatt
     6901 atgaattacc gattgaaagg caggactacc aaatcgtagt acgtagagag aatggttaca
     6961 tagttgggtt gccatttact ttataagttt tattttgtta gtagttttta aggtaatatc
     7021 agttttgtga tttttgaaaa tggaactggg agaatggctg aggaaaataa agttgttccg
     7081 tttgaaacat tagtgataaa taaggaagag tttgagcaac tttttcttga tttttttatt
     7141 caatatgaca tgatttatga ttttagttcg tcactagatt tatttgaatt agaagcagaa
     7201 ataatagaaa acagtcgaga gaagaatggt aaaatcatta tttcagtaga aaaattcaat
     7261 gaattattct cacaaacatt tgaaatagat ccagactatt atcaaccaca agaagttttt
     7321 cgagagttta gaaataagat tattggtttt agtcaaacta caaaatgaaa gcttaataaa
     7381 gcaagtgttg atagaattat tcctcaacgc ttgctttatt ttatgggcac ttcataatag
     7441 aggtatagag aagagaggta ttcattttgg atactattac tccaacaaac ttcaaagaaa
     7501 atataggtaa agtgttgcaa gaagtagttc attcaaattc tgaaatagaa attactaggg
     7561 attctaaaaa tggattgaac gatggcgtca ttatgatttc taagagaaaa tgggagttgt
     7621 tacaagaaga actttattta caacgaacgg gtacatgggc ttatgtatct gatttaatgg
     7681 ataggctaca attaactaga cagaaaaatt aaggtgtgta gactagaaga aaacatacca
     7741 ggaggaattt ttatgtctaa gagaacacga agaacttttt cacaagaatt caagcaacaa
     7801 atcgtcaatc tttacttagc tggaaagcca cgtgtagaaa tcattcgaga atatgaacta
     7861 acggcttcag catttgacaa atgggtaaag caatctaaaa cgagtggttc attcaaagaa
     7921 aaagataatc ttacgcctga acaaaaagaa ttgttagaac tacgtaaaag aaaccagcaa
     7981 ttagaaatgg aaaatgatat tttaaagcaa gcagcgctga tattcggacg aagagacaag
     8041 taatcgatgc gaataagcat ctttacccta tatcagcgat gtgcagaata ttaggtctat
     8101 cacgtcagtc ctattattat caatcaaaac caaagaaaga cgaatcagaa cttgaagaag
     8161 tagtcgctga agaatttatc cgcagccgaa aggcctacgg ctcaagaaaa ataaaaaaag
     8221 ccttatcaaa acgaggcatt cagatcagcc gacgaaaaat tagtagaatc atgaaaaata
     8281 gaggattaaa atcgagctat actgttgctt attttaaagt acatcattct acttgcaatg
     8341 aagccaaaac gacaaacgta ttgaatcgta aattcttaag agacaaccca ttagaagcga
     8401 tcgtaacaga cttgacttat gtacgagtcg ggaaaaaatg gaattatgtc tgtttcattt
     8461 tggatctgtt caatcgagaa attctcggct attcttgtgg agaacataaa gatgccgttc
     8521 tagtaaaaaa agcatttagc cgtatcaaac aacctctgac agaggttgag atttttcata
     8581 ctgatcgtgg aaaagagttt gataaccaag ctattgatga attattaaca acttttgaca
     8641 tcaatcgatc attgagtcat aaaggctgtc cttttgataa tgccgtagct gaatcaactt
     8701 ataagtcgtt gaaagtagaa tttgtctatc aatacacatt tgaaacctta caacaattgg
     8761 atttggagtt atttgactat gtcaattggt ggaaccacct tcggttgcac ggtacacttg
     8821 gctacgagac ac
//
LOCUS       DAGCYL010000115         8805 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.117, whole genome shotgun sequence.
ACCESSION   DAGCYL010000115 DAGCYL010000000
VERSION     DAGCYL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.117"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            2..1342
                     /locus_tag="MB216_002008"
     CDS             2..1342
                     /locus_tag="MB216_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4526807.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1560..2003
                     /locus_tag="MB216_002009"
     CDS             1560..2003
                     /locus_tag="MB216_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBT4526808.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2507..3925)
                     /gene="nhaC"
                     /locus_tag="MB216_002010"
     CDS             complement(2507..3925)
                     /gene="nhaC"
                     /locus_tag="MB216_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBT4526809.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4328..5551)
                     /locus_tag="MB216_002011"
     CDS             complement(4328..5551)
                     /locus_tag="MB216_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBT4526810.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5582..6421)
                     /locus_tag="MB216_002012"
     CDS             complement(5582..6421)
                     /locus_tag="MB216_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBT4526811.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6438..7118)
                     /locus_tag="MB216_002013"
     CDS             complement(6438..7118)
                     /locus_tag="MB216_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4526812.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7120..8193)
                     /locus_tag="MB216_002014"
     CDS             complement(7120..8193)
                     /locus_tag="MB216_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4526813.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8249..8487)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8530..8763)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         2517 a         1810 c         1606 g         2872 t
ORIGIN      
        1 tatggaagat aaaaaattga aaaaacaact tagctccaga catatcacca tgttagcttt
       61 aggtggagca atcggtgcag gtctatttaa aggaagtggc gaagcgattg ggatcgccgg
      121 cccttctgta ttgattgctt ttttgattgg tgggctgatt ttatatatcg tgatgaaagg
      181 tctgggaaaa atcgttttaa gcggtggcga cacgcatcac ggattatctg gtttgatacg
      241 cccttatcta ggtgctcatt ctgctgattt tactgattgg gtctactggt ctatgtggat
      301 tatcaatatc atcgctgaag ctgttgcagc tgcttccttt ttgcagttgt ggttcccgca
      361 cattccagcc tgggtatttg tttttatgtt ggctgtttta actacgatca tcaatcttta
      421 ttcggtacgg ttattcgcag aaacagaata ttggctggct tttgctaaaa tcagtgtcat
      481 tatcttactg atcatttttg ctacttatct tgtcggtcag caaatgttgg gcacaggcgt
      541 tttccctacc cttcagcaat tgactgacca tggtgggttt actcctcatg gtatgaaggg
      601 aatcatcagt tctttgcttg tagttatcta ttcttacggc ggttctgaac tgattgctat
      661 cacggtaagc gaagcagatg atcctaaacg tgcgattcct aaagcaatca aaggtgtcat
      721 gggaagaatc atttcctttt acatcattcc tttgtttttg cttttgattc tttttccttg
      781 gaatacttta gcaggcacga atgtcagtcc ttttgtgatg gtatttgaaa aaatgaatat
      841 cccatttgca gcggatatcg tcaattttgt gattgtcctt gctttgttct catctatcaa
      901 ttcgggtgtg tatgcatctt caagaatttt gttcttccgt ttgaaggatc gaaaaggttc
      961 ctctagaaaa ctagctgttt taaacaagca tcaagtaccg caacgtgctg ttttattttg
     1021 taccagtgtt ctttatcttg gtgtagctct ttcttatttt gtcggtgaca aattatttgg
     1081 ctatctggca ggttcccttt cgtatacagt attattgatc tggatcatta tcagtgcggc
     1141 atcctttgta ctggcactaa gaaaaggaac attatgggat aaaggaatca gcttttttgc
     1201 attagctgtc ttaggattga ttttccttgg tatcctgttc acgaactcca ttggggtaac
     1261 tgtattgact ggcctgcttt acctgttcat ttatttcagt tatcaaaaaa agaatgatgc
     1321 gttcgtattg acgaatgaat gaaatcgata gaaaatttta ttttatttat gtggaataga
     1381 tggagctatt gaagaaagga gttgtgacaa aagtcttgtt gcaactcctt tttccgaata
     1441 aacagtctca tgacctcttg gtaaacggtg ttcaaaaacc ctctgtgcaa aataattgat
     1501 tgttctccat gctcatgaca tgatagaaga aaggaatgaa aaaaaggagt ttgttttaaa
     1561 tgaaaaagta ttggaagttt ttgttgatga tcggtgtttc aacggtagtt atgtttggta
     1621 tgatgtactt gaatgtctat gcgctcgatc atatcttctt cagtcgtaca cgagtattca
     1681 tggcgttgat gatgggtgca gtcatggcag tcatcatgct gttgttcatg tggaaaatgt
     1741 atgaaaacaa aaagataaat gttatgatct tagccgctag tcttcttgtt tttgccggtt
     1801 ccttgtttat ggtacgaagc caaactactg tcaacgacac agcttggatg aaggccatga
     1861 ttcctcatca ttctatcgct atcctggcaa gtaaacgagc tgatttaaaa gatcctgaag
     1921 taaaaaaatt agcggaagaa atcattcagg cacaagaaga agagatcgac aaaatgaaaa
     1981 aaatgatcaa agaaatggaa tgaccattat ccgtccaaat aaaaaaagag gatgttccag
     2041 aagttatcag cttcaagaaa taagaagaag ctacttctgg aacgccgtga ataaggggtt
     2101 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag cttctcctat
     2161 ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag
     2221 gttgtaaaat cagcgttctg acctgagtat tagagagaca aatggaaaaa tagttcctca
     2281 tattttgcca ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcgaga
     2341 ggttgtgaaa aagtcgctta gtttcgcgta ttaagaaaaa ttttgtgaaa tggctttcca
     2401 catttttgta taaatttggc ttaataccga gaaactggct tttgaatacc gtttattcgt
     2461 aaaagggatt gtgacaaaag tcttgtcaca atcccttttt atgtttttat tgttctaatt
     2521 gcgctgtctt gcttactgct tcttgcggtt tcacaaattt ttttgagatt ccccaacctg
     2581 tgaaggcact gaccatcgaa aacacaggag aaagcaaact gaaaaatacg aatggcaagt
     2641 aagtgagtgt aggaatacca aatgtacttg ctacgaaact tccggcaatt ccccaaggca
     2701 ctaagtagtt gattactgtt ccaccatctt ctagcgttct acctaataca gttggatcaa
     2761 atcctgcttt ttcataagtt tctttaaatg cattaccagg cagaataaca gataggaatt
     2821 gttcaccaat aaaaatattt actccgatac ttgtcatcaa tgttgtaaaa atcaaatttg
     2881 gtgtgttcgt catctttctt gatactcgtt ccatcacggt tgagatcaag ccaaattcga
     2941 tcaacaatcc acctaatgac aaagttagaa taatcaatga cacagtaggc atcatacttt
     3001 cgattcctcc gcggctaagc agtgaatcga tttgttggtt tccagtattt gaaacaaacc
     3061 ctgattcgat gaccccagcc aacttcgtta aggaagtttg tgggttttga ataaaaatca
     3121 ttcctactgc tacagcgata ttcaacaaaa tcgttaaaat tgccggcacc tttttccatg
     3181 cacacagtaa cattaaaaat aaaggaatca gtgaccagat tgaaatagta aagtttgctt
     3241 ctagaacatc agtcacttca cgaatagatg aaagacttgc ggctttactt gtgtgtccta
     3301 aaatcatgaa taaaaagaaa gaaacaacaa atgccgggac agttgaccac atcatatgct
     3361 tgatgtgttt gaacaaatcg gcatcaacaa tagctgctgc tagatttgtt gattcggata
     3421 atggagacat cttatctcca aaaacggcac ctgaaacaat agctccaaca accagcgctg
     3481 gatttaagcc tagtgttgtc ccaataccga aaaatgcaat cccaatcgtt gacataacag
     3541 taaaggcact ccctacagcg ctacctacga ttgcacacac aacaaataca gaagggacaa
     3601 accactggac actgatcaat ttgaacccaa tgaccattag agttggaatg atccctgctt
     3661 gtatccatac agcaatcaat gaaccgacta aaataaaaat aaagattggg atgattcctg
     3721 gtttgatgcc attgatgatt ccttcgtgca gccaatctgt cgggtatctt tttatcactg
     3781 catacaacat gaccattccg atcgctgcta agatcgtgat attaggtgag acctttaatc
     3841 cgacaacgcc aatcgcaatg cctaaaacaa taaccattaa tacaagtgtt gcttctaaaa
     3901 aacttggtgt tccttttttt cccatgataa tctctccttt tttagttgtt cttatgttct
     3961 aaaaaagtta agatatcgga ttactccgcc gcaattcatc cgcgattcct cctttataaa
     4021 aataaaaaga cctgttttaa actaaggacg aatgactccg cggtaccacc ttaattaaaa
     4081 acagatctgt ttttcacttt caaactttta ttcaaatgta tggactttcc atgtaattcg
     4141 tcaattaggc ttgatcggtt cacagcgacc accgactttc tgaacaaaac acctaaccgc
     4201 tactgcgtga aaagttgact tgtacgaagt ttaccttcca taagatcatt tgtcaatgaa
     4261 aaagaaaata ttttttcatt tttctgaaaa ttatttttat aactttcaca cacaagttta
     4321 ttttttacta gaaagtagcg ccctcttgtt tttttcttgc tactatttct tgatgttctt
     4381 gcttgattct ttctaatagc tttggctctt tcaacagatc aagagctgta ttggcgagta
     4441 gttctgctgc aatcgcaata gaagcaaggc ctttgtcact tttagcagca gctttgaatt
     4501 cttctgtgtg tccggcaata tatgtctcag agattgctac cgtaggctgg attgttggta
     4561 tgacttggct gacatttcct acatcagaag aaccgagact tgttctttgt tccgtttcta
     4621 tttcttcatc tggaacacct gcttcttttg catgctggaa gaataagtca tcaaaagaag
     4681 gagtaacgat gacatcatca actgcatttt ggaacaatcc aaactcaaaa gtcgtgcccg
     4741 tacttatcgc agcacctcgt acgatatttt ccactttttc atatacttta tctaatgttt
     4801 gccgatttcc tgcacgtaaa tagaaacgtc cagcagcgta atctggaatg acgttggcag
     4861 ctactcctcc gtctgtaatg atcccatgga tattgacatc ttttggcaat tgcaagcgta
     4921 gagcgttgat tccattaaac acttggatca atgcttccaa agcatttact cctttttcag
     4981 gtgctgctgc tgcatgggaa gcgacaccgt aaaatttgat gtcgactgga tcattcgcta
     5041 aagattctgt cgttttccca taacgaaaag ctggatggac gcataacgct acatcgacat
     5101 cctcaaaaaa accttctcga acaaagctgc ctttagctga tccgttctcg cccccttctt
     5161 cccctggggt gccatataca cggatttctc caccagtttc atcaatgaat tgcttcacta
     5221 tgctggcggc taatacggag taagtaccaa acaaattatg cccgcatgca tgaccaatac
     5281 ctggtaaagc atcgaattct gccaaaaagg caattgtggg accgggttta ttggaaatat
     5341 aacgtgcatc aaaggctgtt cggtgtccag ctacttcttt ttctacccaa aacctttctt
     5401 tttctaattg tttaatcaac gctgctgagg aataaaactc atagttactt acttcaggat
     5461 gttcgtggat atctagagca agtgcttgat attctggtaa acgatattgg attagatcct
     5521 tcaattggtt tgttgagatt gttgttgtca taaaaaattc ctcctatgct agtcattttt
     5581 cttattttct cccgaatgtt tcccatgcag gcacgcttga tccttttgat gttttctcga
     5641 ttactttgac tgtgtcttct gtttgataag cgtctactat tttttgatac gttttattgt
     5701 tttcatcttc ttttcttgca acgatgatat tcacataagg acgtgcatgg tcatccactg
     5761 gttctaagaa gatggcatca gaagcaggat taaatccagc atctacagct acaccgctat
     5821 tgatcactga aatgtctaca tctgacaaag cacgtgctgt ttgggaagca tctaattcag
     5881 aaatgtctag attttttcta ttctcggtaa tatcagaaac agtgggcgct tgtccttttg
     5941 ccttatccac tttgatcaat ccagcagttt gcaataacag aagagctctg ccgccatttg
     6001 taacatcatt agggattgct acttttgctc cgtctttgat atcagctaca tccttcaaag
     6061 tgttagaata aattcccagc ggtgcattga cagtatttcc gatcgatact aaatcagttc
     6121 cgtgttcttt attgtaattg tctaaaaaga attgatgttg gaaagaattc aaatcaatcg
     6181 atccatctgc taaagccaca tttggttgat tgtaatctgt aaatttcacg tattccaaat
     6241 taatgttctc ctttttcaaa cggtctttca cgttgtccca tacatctgta tcttctccaa
     6301 taatccctag ttttacgata gtttcttttt tgtcagttgt atctgctttt ccttggctac
     6361 accctgccaa ccaaataatt gctacaaatg taatgactac aaaacgaata atttttttca
     6421 ttttttctcc tccactttca atgtgtaatt tttttaatca gttgattact gataaattga
     6481 cttaaaaaaa ctaagattag aatcaataat gtagccacaa tcgttacatc tgtttggaat
     6541 cgattatacc ctctcgatat agcaagattc cctaatccgc ctgcacctac tgctccagcc
     6601 atggtagtca gtccgatcac gttgatgata gtcaacgccg agacccgaat gataccaggc
     6661 aaaccttcta acaaatacac acgtaaaatg ataccgattg gactggttcc catcgcttct
     6721 gccgcttcga tcaccccagg atcaacttct agcagtgcgt tctcgatttg acgagcaaaa
     6781 aacggaacaa ctccaaaaat caatggcact aaagctgctt tcgcaccaat cgtcgttcca
     6841 actaagaaac gagtagtcaa tgctaaaaga gaaagcaaga taatgaaagg gatggaacga
     6901 atgatattga tcagcttatc aagtatttca aatagcagac ggttagctag taatccatta
     6961 gggcgtgtca ctactaacgt cacacctaag atgattccaa tcatcccgcc aatgaatgct
     7021 gtccaaaaag acatatatag tgtttgcttc gtcgcttcga caaattcatc cggaatcaaa
     7081 taaacattgg gcaaatatgt tttaaagaaa tcagtcattt tatcttcctc cttcgatcac
     7141 tttgatacga ctatctaaat cgtttttttg taaaatattt atttttacac cagatttgat
     7201 caaataatcc aatgcttttt tccgctgttt ggtttctcca gctaaagtaa cgatcaaact
     7261 ccctaacggc gtttcttgta ataactctac attgccatat aaaatattcg ctgttacttg
     7321 atatttgctg tacagatgag cgattagagg ttcgttcgtc tgatttccaa tataacttaa
     7381 ttccaccagc catttattgg cgatctgatc ggcaaaagca gaatgggcaa tgatcgtttc
     7441 taatgcttgg tcaagatgag tagctgttcg aataaaatct ttggtcaatt cttcttcagg
     7501 attgctgaag atctggatgc tgctaccttt ttcaacgatt cttccatctt ccataaccgc
     7561 taccttgttg cagatttctt tgacaacttg catctcatga gtgatcagga cgattgttaa
     7621 acctaactgg cgattcaatt tttttaacaa tgcaaggatc tgaagggtcg ttttcggatc
     7681 aagtgcactg gttgcttcat cacataataa taccttaggt tcattcgcca aagctcgtgc
     7741 gattgctact cgttgttttt gtccccccga cagttgttta ggataagcag atgctttttc
     7801 ttccaaaccg acaagttcca gtagttcatt cactttttga cgacgttcct gtttcgattt
     7861 tccagaatac tttaaagaaa aatcaacgtt gtcaaaaata gttcggcttt ccattagatt
     7921 gaaatgctga aagatcatac cgatggcctt tctttctttt cgcaattctt tgggagagag
     7981 ctgactcaga ttcgtctgat tgacaatgac ttctcctgct gttggtttct gcagcaaatt
     8041 catcacacga actaacgtac ttttccctgc acctgagtaa ccgacgattc cataaacatc
     8101 gcctttttca atcgttaaat ctacagaatc tactgcctgt atctcttttt ctttttgttt
     8161 gaaacataca gaaacatctc gcaattcaat cattaaaatt cctccttatc taaaaaaagc
     8221 gtcctcaaga aaatgaaaat gtcattttct tgaggacgct tttttgcgtg ttaccacctc
     8281 atgttcaagc tgtgttcaca cacaactcct cttcaagtac gatcatactc tagcaagata
     8341 tcggtcgcag ccgtcatcgc cttacatcta tgctcgggat gaattgctcg atggccattt
     8401 tccaatagtc gttccttgct tcttctcagc taccgaagct ctctgtcaaa ggttcttcta
     8461 tcgtacttac catttcaatg caaataatat tcaaaaaaaa agtcctttta ctaacataaa
     8521 ctatgtcagt aaaaggacga cgtatcgcgg taccaccttt tttcggaagt ttccttccct
     8581 caagcccaac atctcaaaaa gagagattgg cgttcgtttt aacggtcgaa cagttccgaa
     8641 tttgcctact tatcgacaaa ttattctcca aggccatttt cagcttttct tacttgactc
     8701 ttctcaccag ccagagcctc tctgcaaagt ttccaaactt actttccttt tcaacgaaat
     8761 cgatattttg ttgttgggat taatctacat gaggaaaact gaaaa
//
LOCUS       DAGCYL010000116         8750 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.118, whole genome shotgun sequence.
ACCESSION   DAGCYL010000116 DAGCYL010000000
VERSION     DAGCYL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.118"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(68..688)
                     /locus_tag="MB216_002015"
     CDS             complement(68..688)
                     /locus_tag="MB216_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBT4526814.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(764..1201)
                     /locus_tag="MB216_002016"
     CDS             complement(764..1201)
                     /locus_tag="MB216_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBT4526815.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            1364..2194
                     /locus_tag="MB216_002017"
     CDS             1364..2194
                     /locus_tag="MB216_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBT4526816.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            2178..3545
                     /locus_tag="MB216_002018"
     CDS             2178..3545
                     /locus_tag="MB216_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4526817.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            3564..4373
                     /gene="yidA"
                     /locus_tag="MB216_002019"
     CDS             3564..4373
                     /gene="yidA"
                     /locus_tag="MB216_002019"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBT4526818.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(4416..4859)
                     /locus_tag="MB216_002020"
     CDS             complement(4416..4859)
                     /locus_tag="MB216_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBT4526819.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            4993..5364
                     /locus_tag="MB216_002021"
     CDS             4993..5364
                     /locus_tag="MB216_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4526820.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(5533..6315)
                     /locus_tag="MB216_002022"
     CDS             complement(5533..6315)
                     /locus_tag="MB216_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBT4526821.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(6312..7283)
                     /locus_tag="MB216_002023"
     CDS             complement(6312..7283)
                     /locus_tag="MB216_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBT4526822.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            7375..>8750
                     /locus_tag="MB216_002024"
     CDS             7375..>8750
                     /locus_tag="MB216_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBT4526823.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEK"
BASE COUNT         2700 a         1789 c         1496 g         2765 t
ORIGIN      
        1 cgtgtcacac ctccttattc tgataggctg atcgtctctg tcacaacctc tcgtaagaaa
       61 ataatgttta ttctttttca gagtcagaaa attcttcctc ttcataatct tcatcatcat
      121 cgtcttcgtc tatgatactt aaatcttctt cgatatttcc tggcaagtct tcttcatcat
      181 ctgtcgggtc atctgctccg atttcttgca agtcagaatt ataagcagcg atttcttcat
      241 cttcatcatc atcgtcatca aacagatcat cgtcatcttc ttctgtcaga tctgtatctt
      301 ctgggtcatc gtcattgtaa tcgatcgcgt cttcatcatc attgataaag gcgttgactt
      361 tcttgcgttt acggcgacgt ggagtatctt catcctcttc gtccattcca tggtttactt
      421 cttcatcgat cgaatcgata gcataccatg aacgcaagcc ccaacgatta tcccctaatg
      481 agataaagct gccgtcaata ttcaagtcag tataaaattg tgcgagtgaa tcacgaattt
      541 cactgtctga tttcccaaga tagttttgga tctggttagc taaatcagaa aaatccatga
      601 catcgccacg ttgttctaag atcgcgtgtg ctacttcgat cattgataat tcgcttttgt
      661 ttgcaccttc aaatacacta atttccaatc aggacacgtc ctttcacagt ctactcctta
      721 tcatacaaaa tcagacacaa aaaatcaaac ttttctttta tatttacgct gtaaaatcca
      781 aggtcaaata atattcgccg attttttccg gtcccatgaa cagatcataa tcgattcgaa
      841 ccttccctgc tgtgggacgg tctttcaaac taacatgcag ttctttcgta aaagtagcaa
      901 tctggatcat accgtatggt gttcgatagg atgtttctaa ccgttcttta tagccaaatt
      961 tcaaacgcat ccgcatttct cctgcacgga tcagctgtac atgaccatct ggaactaatt
     1021 tgatcgttac aggaatctca tcgcccgtct cctcctgaat ctctttataa cgtatgtaca
     1081 gcgtatcgcc aattgtcacc atttgccctt tcaaatcaaa ataaaaatct tgcacttccc
     1141 cttgctgttt tacttttgta cgcaatttga ttgaaatcgg tgttccttgt gataattcca
     1201 ttattcattc tcctttactt ctcatagcta cattgttcct agtttctttt gttttgtcaa
     1261 ccaacttgtc catttttaca gaaaaccttt tttccaaaac aactctttga ttttagagta
     1321 taatattgta gaaaatatga atttatacaa aggagccggt tgaatgaacc ctgcgattcg
     1381 actagatcag cacatttatc aaaaaaacga cttttcccct tttgcattaa cagatatatt
     1441 aacggaatat tctaaagaac accagcaaat ctttttacat ttctggcaat atgactgcac
     1501 agtcatctta ggtatgaagg atgttcgaac tccttttcta agtgacggga tccaaacact
     1561 ggaagatgca ggttacgctc cagtgatcag aaattcaggt ggattaggga tcgtatcaga
     1621 tgaaggaatt ttgaatattt ctttgatttt cccccaaagt gacacaaaaa aaataaccat
     1681 agatgacgct tacgagcaaa tgctcctatt gactaaatac gcttttccaa aggcaccgat
     1741 acaagcattc gaaatcaccc attcttattg tcctggaact tatgatttaa gcatccatgg
     1801 caaaaaattt gccggtatcg ctcagcgtcg gatcaaaaat gggatttctg ttatgatgta
     1861 cttaagtgtc aacggtaatc aggaaaaacg tgggcaagtc atgaagcgat tttatcaatc
     1921 tgctttaaaa gaagactttg gtacaaatgg ctatcctgca gtggatcctg ctacaatggc
     1981 aacacttgaa gaactgcttg accaacccct ttttgtaaaa gaagtcaaag aagcatttgc
     2041 aaaagctttt gaaaagctgt attctgcaag tgatttacag gattctaccg actggctaaa
     2101 aaaagaaatc cagctggagc aatggaacaa ccaaatccag agaatggaac aacgtaataa
     2161 cataaaggag ctacattatg acaacccctt ataaatatca aatgctgcct atggaaaaag
     2221 tttttcgtga tccagtccat aactatatcc atgtccagca tcaagtaatc ttagatttga
     2281 tcaattcgaa agaagtccaa cgtctacgac gcattaaaca attaggcact tcttctttca
     2341 cttttcacgg agcagaacat agccgtttta ctcattcatt aggcgtttat gagatttcaa
     2401 gacgaatctg cgatatcttt tctcgaaatt tttcaaaaga aaaaataggc aacggaggct
     2461 gggatgatca ggaacggctg gtgactttat gtgcggcact gttgcatgat gtcggtcatg
     2521 gtccttactc tcatacattt gaacatatct tccatacgaa tcatgaagcc atcactgtag
     2581 agatcatcac ttctccagaa acagaagtgt atcagattct taaccgagta gaagaaggtt
     2641 ttcctgaaaa ggtagctagt gtgatccaaa aaacttaccc caacccacaa gttgtccaaa
     2701 tgatttctag tcagatcgat gcagatcgaa tggactattt gcttcgggat gcttatttca
     2761 ctggaacaga atatggaaca tttgatctta cccgaatatt acgtgtgatt cgtccttatg
     2821 aaggcgggat cgctttttcc atgagtggca tgcatgcagt ggaagattat atcgttagcc
     2881 gatatcaaat gtatgtgcaa gtgtacttcc atcctgtttc ccgtggtatg gaagtaattt
     2941 tggagcatct gcttcatcgc gcacacgagt tgtatcaaga agatgccagc agttttgaac
     3001 tccattctca attacttctg ccatttcttg acggaagctt tactttagaa gaatacctca
     3061 aacttgatga tggggtcttg ggcacttact ttactcaatg gtctgaatct gctgatccta
     3121 ttttaaatga tttagctaaa cgttttttga atcgtaaacc actgaaatct gctacttttt
     3181 ctggcaaccg ggattcaaaa ttagtccaag aactcacttt gttagtagaa aaggtcggat
     3241 ataatcctgt ttattacact gctgtgaatt ctagctacga tctaccttat gatttttatc
     3301 gcccagaaca aggcagacat cgcacacaaa tcgagatttt gcgaaatgac ggaactttga
     3361 tagaattatc acaggttagt cagcttgttg ctgcattagc gggacaagaa caaggagatg
     3421 agcgattttt ctttccaaaa gaaatggttg atccgtcctt gcgtgatcat tatgatttat
     3481 ttgacgaaac ttatcaagaa tttgccagcc atatccgtaa tggcgcattg attgaaataa
     3541 attaaaatat attggaggaa attatgtcta tcaaactagt cgctatcgac atcgatggta
     3601 ctttattaaa tgagaaaaga gaagtaacaa aagaagtaaa agaagcgatt gccgctgctg
     3661 ttgcaaaagg tgtttcgatc gttttatgca ctggccgtcc cttaccaggt gtccaggaac
     3721 agttaaacga gttgaacctt tttcaagata atgattacgt tattacttat aacggggcac
     3781 tcgtacaaca aacgaaatca ggtaaaatca tcgcccgtca cggactgaca catgaagatt
     3841 tcctagagat cgaagtaatg gcacgccgtg tcggcagtca tctccattcg atcgatgaca
     3901 aagcgatcta cacgcctaat cgagatatca gcgcttatac gatccacgaa gcaagtttag
     3961 tcaagatgcc gctaaaatat cgaacacctg aagaaatgac accagacatg aacatcgtca
     4021 aaatgatgat gatcgacgaa ccagaaattt tagatgcagc aattgcgcgt ttaccgcaaa
     4081 ctttccgtga taaatacaca acggtcaaaa gtgcgcccta ttacttcgaa gtactaaata
     4141 aagaagcaag taaaggtgcg gcagttgcga atctcgcaca gcaccttggt attgatcaag
     4201 atgaaatcat ggctatcgga gataacgaga acgacttatc catgattgaa tatgctggtc
     4261 taggagtagc aatgggaaat gccgtttctc ttgtcaaaga agcagcaaat gtcatcactt
     4321 catcaaacga cgagcacggt gtagctgaag caatcaaaaa atatgtactg taataaataa
     4381 aaaaactgag agaaagctga tctactcgct ttctttcagt ttttattttt ttcgatccat
     4441 ctctcgactg cccatcggtg gctaccacgt ttcaatttca caagcgcttc ttgagggctc
     4501 acccaccaaa ttttattggt cttttcagtt ggttctccta tacgcttcca agaatcagca
     4561 acaaagaaat atcctggatt ataataataa gttgcacgat aattcgagta aaaatactca
     4621 tcagcttctc ccagataaga agaaatttct gccacaattc ccatttcttc tatcagctca
     4681 cggacaatcg cttcttcttt cgtctcttct ccttcaattt ccccgccagg aagaaaaaaa
     4741 gcaccatttg gagcctcgat tactgcaatc tgctgatctc tttctatgac aatatatgct
     4801 gcataacggg tttgataatc agcaaattgc tttttttcac caaaaattgg tttatccatg
     4861 aatagtttcc accttaacag ttttattttc tatccatttt acatgagaaa tttctcatga
     4921 acaagcacaa atcaactatt tccttacttc tttttgttat gattgagtaa aaggaaaagg
     4981 agagatgaat ctatgaaaat ggctcacaca tgtgttcgag tgaaagatct tgaagcatca
     5041 atcgaatttt atcaaaaggc atttggtttt gaagaaagcc gccgccgtga ttttccagaa
     5101 aacaaattta cacttgttta tttgacactt cctggggatg actatgaatt agagttgact
     5161 tacaactatg atcatggtgc gtatgatcta ggagacggct atggtcatat cgctatagca
     5221 gctgatgatc tagaaaaatt acacgagcaa cataaagctg ctggtcttga gattactgac
     5281 ttaaaaggat tacctggcac tgcgccttct tactactttg tcattgaccc tgatggttat
     5341 aaaatcgaag tcatccgagg atagttagta tggggcattt tactcttgat tgaataaaaa
     5401 acaaacacca ataattaaaa tgagacgcaa acttttcatt aaattgaaaa agtttgcgtc
     5461 tttttttaac aaccattgta gctataaggt tgccactctc caccaattta tttttaattc
     5521 atttgaaaga aatcaaataa acgaggtacc tttagaaaac aaaactgctt ttcctcctat
     5581 tatcacacga tcttctttta attcacattc tagatccccg ccccttgatg acatttgata
     5641 agctgacaat tttgttttat tcattctttc tgcccaataa ggaataagat tagaatgtgc
     5701 tgatccacat actggatctt cattgattgt taattttgga aagaaggttc tagaaacaaa
     5761 gtctttttgc acactttctg ctgtcacgat gactcctatt cctaattcaa aatttttgat
     5821 agctgtaaaa tctggctgca atttagccac cgttttttca tcttctaata cgaaaaataa
     5881 atcacgtgct aagtaggctt ctttaatctt agccccaatc gcttcttcat attcagacaa
     5941 tatcggcact tttttcggca taatactagg aaaatccatg taataataat ctttaattct
     6001 tgtcacaaat agattaccgc tttgagtaga aaatttaatc gtttcatcag gtattttgta
     6061 atagttaaat aaaacaaatg ctgttgctaa tgtcgcgtga ccacaaagat cgatttctcg
     6121 atctggcgta aaccatctaa gctcaaattc ttggtttttc ttcactgtaa atgctgtttc
     6181 agataaattg ttttcaatgg caattttttg catcgtacct tctggcagcc attcttctag
     6241 cacataaacc gctgctggat ttcctttgaa cacctcatct gcaaatgcat caactacgta
     6301 ataatctaag ttcattttaa agcccctttc tctaatttgt tataaatata ttctgcacaa
     6361 caaagatctt caatagctag accaactgcg tcgaaaatcg tcggcctcga ttctccttta
     6421 taaaatgttt ttttctcaga aactaattcg cctaatgtac caacaataca agattcctct
     6481 aaatctcctt ctgccatggc tataagcagg tccccgcttt ctgttaaggc agctgagtaa
     6541 tcatctacat agatttgaga agtttccatc aacgatgtac ttatctctct tgtatcagga
     6601 gtaaatgtgc ccacagcatt gatatgtgcg tcttccctta cccactcttt ttgaagaaat
     6661 gcctctttac tagaggtcaa cgtacaaatg atttccgctg gacgtactgc atcttctact
     6721 gatattgcat ttataaacgt tattttagga tactgttttt tagctttagc gataaaagta
     6781 tttcgtcttt cttctaaaat atcgtaaaca aacactgtag tgatgggacg aatggtagaa
     6841 atcgcagtca aatgcgacaa tgcttgttga ccagctccaa ttaaagcaag tgtttctgca
     6901 tgcggatttg ctaagtaatc tgttgccagc gcagatacag cagctgttcg aatccaggtg
     6961 actgcatttg cgtttatcaa tgctttgggt agtccgtttt gagcatcaaa caagaaaatc
     7021 tctcctaaat gagaaggtaa atgatactga tgattatccg gaaaagcggt aatgactttt
     7081 gtgccaaaat atctgttctc ccccaaataa gcaggcatca tcgcaaacat atttttgcct
     7141 tttccgtcag gtaaaatttc caccaatcga ggattttggc tgattttacc actttcataa
     7201 tcaaaaaaac attttttcat tacttgaatc gcttcttcaa aactaaaaga agctactact
     7261 tcctcaaaat taataatttt catagacaat tccctcctca caaaagatta acttctaaaa
     7321 tcaatacaat caatggtaaa attgtatggt atacaatttt aaggagtgga gaaaatgcct
     7381 attaattctt atgacaatta cccgttaact tggcgaccag acaaacaaaa attgaagcgt
     7441 ccttattatc gatcgttgat tcaacaatta gaacaagata ttttaaagaa taaattacag
     7501 aaaaacactc agttaccctc ccaaagagag ctagctgatt atttagattt aaattttact
     7561 accgttggca aggcttataa atatggaatt gagaaaggtt tgctgtatac gaatattgga
     7621 agtggcacat ttatttctcc caatgtcttt gagtccatca ccatctctac caatgaagta
     7681 accgaacata tcattgattt tggtctggtt agttcttttg aagaatgtaa taaattcgtt
     7741 gtcccgttta ttcaatcaac tagtaataac ccaaatcttg ttggtttgct taattaccaa
     7801 gatcccaaag gcacaaattt tcaattgact actgcagcaa attggttaaa aactcaagga
     7861 gtcattccgt ccttagaatc tctatctatt gtttccggtg ttcaaaatgg attggctatc
     7921 acactttctg cacttttctc tccaggtgat agaatagcaa cagaccgata tacatacgct
     7981 aatttcattg aattggctca gttatatcat ttagagatcg ttccaattga ttttgacgag
     8041 caaggaatgc gacctgatat attgattcag gaatgtagaa aaaagaagat tcatggtgtc
     8101 tttcttatgc cttcttgtaa taatcctata gggtttcaaa tctctcacaa aagacgagag
     8161 caattagccg aagtgattga aaaagaaggt ctatgggtta ttgaagatga tatccattca
     8221 tttttaacaa cttactatca acatacgatc attcctcctt tccaaaattt attacctaac
     8281 caaacaatct acttagctgg aatgacaaaa tttatttgtt ctggattgcg agttgcttat
     8341 cttgtagttc cagtacatgt ccaaagcaag atagaacaag caatttttaa tataaatgtg
     8401 aaaacttctg gattagatgt ggaagtcatc actcaaatcc tccattcaaa tgaagcaaat
     8461 aggatcttag aaactaaatt aagtcttaca aaaagagcca atcagctttt tgattctctt
     8521 ttcaaagtag aaaaaccaac gaatcgtgtc ccttactatc gaattcttcc agttgatcct
     8581 gagattgacc ctaagataat tgagaatgac tttcttgcta acggtatccg actttatcat
     8641 tccagacgct ttactacacg tgcgcaacca gatgcattcg ttcgaatttc cctatcctct
     8701 aataaccttg aaattttagc ttctggacta agtatcgtaa aagaaaaaat
//
LOCUS       DAGCYL010000117         8746 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.119, whole genome shotgun sequence.
ACCESSION   DAGCYL010000117 DAGCYL010000000
VERSION     DAGCYL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.119"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(871..1284)
                     /locus_tag="MB216_002025"
     CDS             complement(871..1284)
                     /locus_tag="MB216_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBT4526824.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1740..2225)
                     /locus_tag="MB216_002026"
     CDS             complement(1740..2225)
                     /locus_tag="MB216_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526825.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2364..2579)
                     /locus_tag="MB216_002027"
     CDS             complement(2364..2579)
                     /locus_tag="MB216_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBT4526826.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2803..3603
                     /locus_tag="MB216_002028"
     CDS             2803..3603
                     /locus_tag="MB216_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4526827.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3622..5490
                     /locus_tag="MB216_002029"
     CDS             3622..5490
                     /locus_tag="MB216_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBT4526828.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5483..5974
                     /locus_tag="MB216_002030"
     CDS             5483..5974
                     /locus_tag="MB216_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBT4526829.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5962..6516
                     /gene="srlA"
                     /locus_tag="MB216_002031"
     CDS             5962..6516
                     /gene="srlA"
                     /locus_tag="MB216_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBT4526830.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6534..7529
                     /locus_tag="MB216_002032"
     CDS             6534..7529
                     /locus_tag="MB216_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBT4526831.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(7671..>8746)
                     /locus_tag="MB216_002033"
     CDS             complement(7671..>8746)
                     /locus_tag="MB216_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBT4526832.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         3016 a         1420 c         1672 g         2638 t
ORIGIN      
        1 taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta atacatttta
       61 atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag agagatcata
      121 agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc ccatatttct
      181 tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat ccagcaccat
      241 ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg aagtaaaaac
      301 aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc ccccacattc
      361 aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt atgaataaca
      421 gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga agtttatttt
      481 tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa gccgatttcg
      541 tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt atggataaaa
      601 tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa taagtaaaag
      661 aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa gacaatcaaa
      721 tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt aaaaacaaaa
      781 tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat atataaaaat
      841 cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt ttgtttcttt
      901 tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa aggttccagt
      961 gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga ccgaatgtct
     1021 ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat gattggcgga
     1081 gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac agccaggcat
     1141 atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg gataacttga
     1201 aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag ggaacatgtc
     1261 gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt ttttatttat
     1321 ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt agctttgtga
     1381 atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg atcattaaaa
     1441 taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca aatatagaaa
     1501 aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac cgaaaatgaa
     1561 gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc ataagagaaa
     1621 tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta taactttctc
     1681 agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat atcatctaat
     1741 caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga cactcgataa
     1801 aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc tgtgaacaaa
     1861 taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg taattcggaa
     1921 cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag agaaggtaca
     1981 gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact tgattggcta
     2041 tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga agtactttta
     2101 gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga attctttgtt
     2161 ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta aatcccttta
     2221 tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt aaacaaagaa
     2281 aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat gaagataata
     2341 acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt aatcattggg
     2401 aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat cttcgcaaaa
     2461 atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa gcctaccaac
     2521 agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa agttttcatt
     2581 agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa cattgcttat
     2641 ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt tgctcttcca
     2701 ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt ttagtgagct
     2761 tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga ttggttggat
     2821 attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag aagtatcgtc
     2881 gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga atgccgtata
     2941 cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga catagaagaa
     3001 gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa taatgcagga
     3061 ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta tgaattatca
     3121 gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt gatgagccaa
     3181 gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct ttcttctgaa
     3241 tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc agcgatgaac
     3301 agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt ggtaggtgtt
     3361 gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga agctctttct
     3421 tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac aagtacgatc
     3481 cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta ttatctttca
     3541 gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa aacaagagga
     3601 taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt attgaaatta
     3661 ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa aaccagtaat
     3721 caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat tgcaaaaatc
     3781 atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt tgatgagatc
     3841 atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag aatcgcttat
     3901 ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt gtctgaagaa
     3961 gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga gatgatcaat
     4021 gattttgatg taaagattac aggcacacca aataaaggac taagaataat aggagatgag
     4081 ctagaattac gactactttt attttatttt gtttctccct acttttcaga gacgaaaaca
     4141 gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac gaatgctagt
     4201 aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag aatctctagt
     4261 aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata tatccaagaa
     4321 ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag ccaatatgaa
     4381 aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat gataaggaaa
     4441 gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg acgattagat
     4501 ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct ctctttattg
     4561 ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga gatagaaaaa
     4621 aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga agctatcttg
     4681 caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga gttagcattt
     4741 cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac gacaggaaaa
     4801 ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga tatacaaata
     4861 acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt cgctgttttt
     4921 acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt gactaatctt
     4981 tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg attagccggg
     5041 tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta tcaagaatat
     5101 ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga atttgaaaaa
     5161 aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt tgcttttcct
     5221 cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat aactgatcaa
     5281 ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc tgaagaaatg
     5341 acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga actcataaaa
     5401 atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt aaaactaatc
     5461 aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt tcgcagcttc
     5521 agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca atcaaacata
     5581 tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga aaatccgttc
     5641 aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag tcagaaaaat
     5701 gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg gtgaagatat
     5761 ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga cacaagcagc
     5821 tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa aaagtgaaca
     5881 accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa gtacgatcaa
     5941 agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg atttatgagg
     6001 ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt accagttgtc
     6061 ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga ccgagtaacg
     6121 aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt accgttctta
     6181 tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct tccagaatac
     6241 tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa tggtgtattc
     6301 ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg gatcgaaacc
     6361 ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact gctgatgaac
     6421 tttattggtg gttggataac tgattttact actggatttg tatgcagaca gcaaggaatc
     6481 gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt taaatgtatg
     6541 gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt attaggccga
     6601 caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca atgatcgtag
     6661 ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca tcaatacctg
     6721 atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt ggaatttatc
     6781 ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc ccattagcac
     6841 aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata cagccactaa
     6901 tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa aagtacaaat
     6961 atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg atagcaaaaa
     7021 ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga gaagcagtag
     7081 atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg attggtgtga
     7141 tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta gcaggaaatg
     7201 cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct cctttgcttg
     7261 gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag attgggaaag
     7321 gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact caaaatgcat
     7381 gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca gtagaagtag
     7441 gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt atcattgctt
     7501 gggtagcaag ttttggatta tatagctgag tttagtctaa tggtgataag tcaagatgaa
     7561 atttaagatt ttctttattg agtaatatgt tttgaagcgc acgaaactag acccgatcaa
     7621 aatcaaattt ttttcgggtt gattcaagga tttgcgtcaa taacggattg ttatttctct
     7681 atcacactcc ttggtggcgt atagagttgg tgtttacgta gtagcgtatc caccagacgc
     7741 gtaaattttc ttgcggttag gacgagtgca cgtttgtgtt gatgctttgg aacctcctta
     7801 tatttacttt gataaaaagc tttgtattca ggaagataat tttttacaga gttggcagct
     7861 tcaactaagt agtatctaaa atatcgattt ccttgttttg tcagaggtgt attttgcgac
     7921 tgatagtttc cagattggtt cactttccaa cttaatccag cgtattttgc caattttgtc
     7981 tgatcttcaa atctttcgat ttgaccaatt tcagcgatta aaccggcagc atagacttta
     8041 ccaacgcctg gaatactggt taagcattga tattctggaa tgacaacaat tacttgttcg
     8101 atggctttat ctaattcttt gatatttttt tcaagtgctc gtatttctcg tacgagaaca
     8161 cttaaaacaa cattaattga ttcttgtgca agagcaccta ggcggtaact catggtaatt
     8221 gcacgttgaa ttgctttagc gattttttct ggttgcttga aacggccttt tcctttttct
     8281 tgaagtaaat cacagaaagc ttctaaagga agctcagcaa gttcatccaa tgtgaaatct
     8341 tccgtcatga gtgaaatgag ggttgcacta aaaagactcg tcgattcgtc tcgcatttct
     8401 cgagcaagcg tattacattt gtacgttaga ttttcaagaa aatgttgctt tgtacgaatc
     8461 aattgcttaa taagttgata gcgagtacgt gttaaacgct gtaaggccat gtatttctct
     8521 tctttgatag gggaagttgt gaatcgttga atacgcaaga aatcagcgat tcttaatgca
     8581 tcgtttcgat cagttttatt ctcatcaaac atgcgactaa attgtttcac ccgaaaagga
     8641 ttttcaatcg ttacaagtgt gttgagcttc tttagtttct catcttcatg aaaaaacatg
     8701 gaaggatgga agctgtacat ggacgttgac tccatgccaa tcacaa
//
LOCUS       DAGCYL010000118         8734 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.120, whole genome shotgun sequence.
ACCESSION   DAGCYL010000118 DAGCYL010000000
VERSION     DAGCYL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.120"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            132..1535
                     /locus_tag="MB216_002034"
     CDS             132..1535
                     /locus_tag="MB216_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBT4526833.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            1535..2968
                     /locus_tag="MB216_002035"
     CDS             1535..2968
                     /locus_tag="MB216_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBT4526834.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            3113..4039
                     /gene="trxB"
                     /locus_tag="MB216_002036"
     CDS             3113..4039
                     /gene="trxB"
                     /locus_tag="MB216_002036"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBT4526835.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYGSVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            4253..5980
                     /locus_tag="MB216_002037"
     CDS             4253..5980
                     /locus_tag="MB216_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBT4526836.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            5995..6345
                     /locus_tag="MB216_002038"
     CDS             5995..6345
                     /locus_tag="MB216_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBT4526837.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(6437..7297)
                     /locus_tag="MB216_002039"
     CDS             complement(6437..7297)
                     /locus_tag="MB216_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBT4526838.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRVFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            7389..7817
                     /locus_tag="MB216_002040"
     CDS             7389..7817
                     /locus_tag="MB216_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBT4526839.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            7979..8155
                     /locus_tag="MB216_002041"
     CDS             7979..8155
                     /locus_tag="MB216_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBT4526840.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            8182..8628
                     /locus_tag="MB216_002042"
     CDS             8182..8628
                     /locus_tag="MB216_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBT4526841.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         2932 a         1479 c         1952 g         2371 t
ORIGIN      
        1 acaagatatt tacaaaaatc cactagaaaa cagtaaattt tgtaaatgtt actggaaaga
       61 aacagatttc tattgtttgt tttgtttaca aaaaagataa tgaaagtgta aaaaagtaaa
      121 ggagtgagaa catggcaaaa ggaatcaaaa tcgctacgat tggtggaggc agcagttaca
      181 cccccgagtt gatggaagga tttatcaaaa gatatgagga attaccgata cgagaaatct
      241 ggctcgtaga tgtagaagaa ggaaaagaaa agcttgagat cgttagtcag atgtctcagc
      301 gtatgtggga tgcttctccg tatgaagtca agatctatgc aactctggat cgggaagaag
      361 cattgaaaga tgcggatttt gttacgactc agtttcgtgt cggtcaatta gatgcacgaa
      421 tcaaagacga gcggatccct agttattatg ggatgattgg gcaagaaaca aatggtgctg
      481 gcgggatatt caaagctttt cgtaccgttc cagtcgtttt ggcaatcgtt gaggacatga
      541 agcgtttatg tccaaatgct tggctgatca atttcgcgaa cccaagtggg atggtgacag
      601 aagcagttat cagatatggc aaatgggata aagtgattgg attatgcaat gtgccagtga
      661 tggcgatgat gacagaacca gcaatgcttg gcaagaaaaa agaagagctg atctacaagt
      721 ttgccggtct aaatcatttc cattggcaca aagtagcaga taataaagga aatgaccgca
      781 cggcagaatt gattgatcgt ttatacgatg aagagaatgg attaccgaaa aatatctttg
      841 atatcccgtt tttcaaagaa caattaaaac aaatgaacat gatcccttgt ggctaccacc
      901 gctattacta tcgtgaagaa gaaatgctgg aacatgcttt agaagaatat caaacgattg
      961 gtacaagagc gcagcaagtg aaagaaacag aagcagagct atttgaatta tacaaagatc
     1021 cagaattaga ccataaacct gaacaattgc aaaaacgagg tggcgcttat tattcagacg
     1081 ctgcctgtga aacgattgct tcaatctatg gaaataaaga aacacagatc gtggtatcta
     1141 caaagaatga tggagctgtt cctgacctgc cggcagattg tgtagtagaa gtcactgcct
     1201 atgttggcgc acaaggagcg agaaatgtag cgtttggttc tcttcctgct gctgaaagag
     1261 gctggttgca agtaatgaag aatatggaat tgctgacgat cgaagcagct gtgactggtg
     1321 attatgggac agctttacag gcgtttacga tcaatccact tatacgatca ggaaagacag
     1381 caaaacggat catggatgag ttgtttattg cacaccagca gtatttaccg caattcaagg
     1441 agacaatcag ccgattaaaa gaagaaggaa tcgaagttca agatgaactg gcaagagaat
     1501 tatcaggtaa tacacatata atggaggaag tataatggga ttcagaaaag attttttatg
     1561 gggcggtgct acagcagcca atcaatgtga gggcggttat gatgaaggtg gacgcggact
     1621 agcaaacgta gatgttgtgc caatcggaaa agaccgtttt cctgttatca ccggaaagaa
     1681 aaaaatgttt gaatttgatg aggatcattt ttatccagca aaaattgcaa tcgatatgta
     1741 tcatcgttat aaagaagata ttgctttgtt tgcagaaatg gggttcaaga cttatcgaat
     1801 gtccatcgcc tggagtcgta tttttccaat gggggatgag aaagagccga atgaagctgg
     1861 cttgaaattt tatgaggatg cctttaaaga atgccaaaag cacggaattg agccacttgt
     1921 gacgattacg cattttgatt gtccaatgca tttgatcaaa gaatatggtg cgtggcgttc
     1981 cagagaactc gttggttttt atgaaaatct gtgtcgagtc atcttcaatc gctataaagg
     2041 acttgtcaaa tattggctga cattcaatga gatcaatatg atccttcatg caccattcat
     2101 gggcgcaggg ctgtattttg aaaaaggaga aaatgaagaa caaatcaaat atcaagcggc
     2161 acatcatgag ttggttgcaa gtgccatcgc aacgaagatt gctcatgaag tagatccgga
     2221 gaacaaagta ggctgtatgc tggcagcagg agcatattat ccatacactt gcaatcctga
     2281 agatgtttgg gcttctagaa aatcagatcg agaaaattat ttcttcattg atgtccagtc
     2341 acgaggagaa tatcccgctt atgcattgaa agaaatggag cgaaatggca ttcagatcga
     2401 aatggaacca ggggatgccg cattgctgaa agaacatact gttgatttta tctctttttc
     2461 ttattatgct tcccgagttt cgactaccga caacgaactc ttggaacaga ctgcaggaaa
     2521 tatctttgct tctgtcaaaa atccatatct gcaagcaagt gaatgggggt ggcaaattga
     2581 tccattaggc ttgagaataa cgatgaatga tatttatgat cgttatcaaa aaccattgtt
     2641 tattgtagaa aacggcttag gtgcagtcga tacgccagat gaaaatggtt atgtggaaga
     2701 tgattaccga atcgattacc tggcagcgca tatccaggcg atgaaggatg cagtagaact
     2761 agacggagtc gatctattag gctatacgac ttggggatgt attgatttag tatcagctgg
     2821 taccggtgag atgaagaaaa ggtacggatt catttatgtc gatcgtgata accaaggaaa
     2881 tggcacatta gcccgttcaa agaaaaaatc attcaactgg tataaaaaag taattgattc
     2941 aaatggagaa aatctttcga acgattgatc aatgatttat aaaagtctat tgtttttcag
     3001 cggatttttc gagtataata aagctattcg agaaacgcaa gcagaacaat agactttttt
     3061 tcgcattggc tgtaaagtat gcttacttga ttaagtaaag gagaatctac atatgtatga
     3121 tgtaatcgtt attggcgcag gtcctgctgg gatgactgct gctttatatg cttcacgttc
     3181 aaacttatcc gtattgatga tcgaacaagg tgcccctggc ggccaaatga acaatacggc
     3241 agaaatcgaa aattatcctg gattcgattc gatcatgggc cccgatctag cttataagat
     3301 gtatggaagt gtctcaagat ttgggacaga aaatgcttat ggaatcgttc aagatatcaa
     3361 ggatcacggc agccataaag aagtcatctg tgaagataaa agttacgaag gaaaaacagt
     3421 cattatcgca acaggatgta tccaccgtaa attaggcgta cctggcgagg aagaatttgc
     3481 tggacgaggc gtttcttact gtgcagtctg tgatggtgcg tttttccgta acaaacgtct
     3541 agtagtcatc gggggtgggg actcagcagt agaagaagcc atctatttga cacaatttgc
     3601 ttctgaagta gtgatcgtcc accgtagaga tgaattacgt gcacaaaaga tcatccaaga
     3661 ccgtgcattt gcaaatgaaa aaatttcctt tatgtgggat acagtcgttg acgaaatcgt
     3721 tggaaacgaa atggtagtga caggtgtgaa agcacgtaat gtcaaaaccg gcaatgaatt
     3781 tgatttagca gcagacggag tattcatcta tgttggactg gatccattaa ctgcaccttt
     3841 tagagaagca ggtttgacga acgaagctgg ctggattcca acagacaacg aaatgaaaac
     3901 gaatatgcca ggcgtttttg ccatcggaga tgttcgtgaa aaagatttgc gacaaatcac
     3961 tacagctgtt ggtgacggcg gtattgctgg acaacaagtg tataaatact tggagtcttt
     4021 agaagaaaca acagcataag tttaaaaaga gtagaagaac gggttcaatt gttcttttac
     4081 tctttttttt cgcgtgaaat ttactgaatc tgatttatgc gaacacgtcc aacacctttt
     4141 gataaaagaa tcctttcttg atttaagcgt tttttggtcc tcatactttc atatctaata
     4201 agtgaatgct atacttatta agaaccatat ttcagaaaga ggtgttattt tcatgtcttg
     4261 ggaacaagtg tatgaacaat gggtacatga agaaaattta gaagaaaact taaaaaaaca
     4321 attaaatgat ctgggtgaag atcctgaaaa acttgaagat gctttttatg caccgttgga
     4381 attcggtact gcaggtatgc ggggaatctt aggacctggg atcaatcgaa tgaacatcta
     4441 tacggtaaga caagcaacag aaggactagc acgtttcatg gatacacaag gtgaagaaac
     4501 aaaacgccga ggtgtcgcta tcgcttatga ctcacgccat cagtcaccag aatttgcaat
     4561 ggaagcagcc aaaacactag cacatcacaa tatcccgtct tatgtattcg aaagtctgcg
     4621 tccaacacca gagctttctt ttgcagttcg tcatttgaag acgtttgcgg gaatcatggt
     4681 aaccgcatct cacaacccag cggcttacaa tggatataaa gtctacggag aagatggcgg
     4741 acaaatgcct cctgcagacg cagatgcatt gacgaaatat gtccgtgaag tcagcaatcc
     4801 gctaaaagtc gaagtattat ctgacgaaga agctaaacat agcgggttga tcacgatcat
     4861 cggagaagaa gtggacgctg cttatctaga agaaatcaaa gcagtgacga ttgatcgtga
     4921 attggttgaa acaatgggga aagatttgaa acttgtgtac acgccacttc atggaacagg
     4981 gaaaatgttg ggcgaacgtg cgttgaaaca agctggattc gaacaattcg tactagttcc
     5041 cgaacaagct gtggcagatc cagatttcac tacggtcaaa tcgccaaatc cagaagaaca
     5101 ttcagctttt gaatatgcta tacgacttgg agaaaaagaa ggagctgact tgctgattgc
     5161 aacagatcct gatgcagacc gtttaggcgc agcggttcgt ttgccagatg gttcttatga
     5221 agtattgact gggaaccaaa tcggagcatt gtttgctcaa tatattttgg aagcacatca
     5281 atcaagaggc actttaccag aaaacgcagc ggtattgaaa tcaatcgttt ccagcgaatt
     5341 gcctacagca attgcggaac actacggtgc taccatgttc aatgtgttga cagggtttaa
     5401 attcattgct gaaaaaatcc aacaatttga agaagaccat tcacatactt ttatgtttgg
     5461 attcgaagaa agttatggtt atttagttaa accttttgtt cgcgataaag acgcgatcca
     5521 agcattgctt ttattagcag aagtagcagc ttactataaa aaacaaggca aaacgttgta
     5581 tgacggcttg caaacgattt ttgaaaccta tggctatttc gcagaaaaaa cgatttctgt
     5641 gacaatgagc ggacaagaag gttctgcgaa aatctctgct ttgatggaaa aattccgtaa
     5701 agaagcaccg acagaatttg ccggagcaaa agttattcaa acagaagact ttaaagaatt
     5761 gacgagaaca gaccgtgaag gaaacgtaga aaaactaaca acaccgccat ccgacgtatt
     5821 gaagtataca ttggaagaaa atggctggat cgctatccgc ccttcaggaa ctgagccgaa
     5881 aatcaagttt tatattggtg taaaaggaag ctctacagaa gacgcagccg aaaaagtagc
     5941 ctcttatgaa gcgattatca aaaagatgac agaagaataa aaaacttgag actgatggaa
     6001 aaagtggtaa actttagaag tcagtctttt ttcaagttgg aggtgaatgg attgccagac
     6061 acgcaaaaat caaaagtaga gaaagtcagt caattattca aaatgttgag tgatcccact
     6121 cgtcttaaaa ttctgctata tctgaaagac ggggaacaaa atgtgacagc aatcacccaa
     6181 gcagtggaaa tggagcagtc tgctgtatcg catcaactac gtttattaag agaaaatcat
     6241 gtcgtcaagt ctcatcgcga gggaaaagcg attttgtata gtcttgatga ttctcatgta
     6301 ctggatattt tgaaccagac actcaaacat gtggaacatc aataacgata ggtgaaaata
     6361 gataaacgct ggagtgtgac aacgaagaaa tttcgttgtc acactccagt gttttttaag
     6421 aaaaagacaa aaatgattaa tcgctttttt ctgttgtttc agttccataa taactatgat
     6481 cttctaggtc taaagcattc aaaataagcg aagccgtttc ttcaatagat aaagatgcca
     6541 cattgatcac tacacatcct agtttttcat aaagatcatt cgcaaaatcg agttcagctc
     6601 taattttttc gatatcggaa taagcagtat cagggtttaa tccgtaagag cgcatccgtt
     6661 ctttacggat attatttaaa atatctgggt tattcgttag tcccacgatt tttttaggat
     6721 cgatttccca cagctgtttg ggaatatgtg cttggggaat caaaggtaaa ttagcaactt
     6781 ttaaattttt atttgctaaa aacaagctca aaggcgtttt ggaagttcgg gatacgccta
     6841 gcaaaacgac atctgcttct aaaaaaacac gaggatcttt cccatcatcg tattttacag
     6901 caaattccat tgctttgatc cgtttgaaat agttctcatt caataaatgg agggcaccag
     6961 gttgccgaat tggcttgata ccagttaaac gttcaatttc tgatatcggt ggcgtcatca
     7021 catcgaaact aaataattga tgttcttcac aaaaagaacg tgttatctcg atcagttctt
     7081 gattgattaa tgtatgcagg acaagtgctt tttcttctaa tgcatcttgc aatgcctgcc
     7141 ttaatgtttt ttcatctgtc acgaacgttc tgcggaatag gttgaactca acggtaggat
     7201 actgagccat agtagcttgc gctagttttg tcgcagtttc tccagcagag tcggagatga
     7261 caaaaaaagt aacgatttct gtagaatcgg aagacatgga taaaacctca cttgcatttt
     7321 ttttatgttt attataccat actctagaat gcgtacttcg taatcatttt gatttggagg
     7381 aataagtcat gaaaaaacag acattgatcg aacaatcatt agaaacattg aaaaataacg
     7441 gttttaaata tactaaaaaa agagaagcat tgttaactta tttagtgaag cgtaaccgct
     7501 acgtatctgc taaagaagtt tttgactata tgaatcagga attcaaaggg gtaagttatg
     7561 atacgattta ccggaatctc catgattttg cagagatcga tttactggaa gaaaccgatt
     7621 taaatggtga aatgaagttt cgttttcatt gctgtcaggg ggaaatcggc catcaccatc
     7681 attttatctg tacagtgtgc ggaacaacga aagaaatcca tatgtgtcca atgaattatt
     7741 tcgaagaaca gctaggtgga tgcacgatcg aagggcatag attcgagatt ttcggaagat
     7801 gtgaaaattg tcaataagga tttaaataaa ataacatttg ataatttaaa aaaaaagcaa
     7861 gatttccttg acggtcaaaa cagcattaga tataatattt acaggacagt cttgtttttt
     7921 gagatatatt tcaataacaa ttacggttac tttttaagct cggagggagg gaattaacat
     7981 gtcaaaaaca gtcgttcgca aaaacgaatc acttgacgat gctcttcgtc gcttcaaacg
     8041 ttccgtgtca aaagcgggta ctttacaaga atctcgtaaa cgtgaattct acgaaaaacc
     8101 aagtgtaaga cgcaagaaaa aatctgaagc agctagaaaa cgcaaaaaat tctagttctg
     8161 tgatgaattg gagttggtag tgtgtcacta ctaagtagat taaacgatga catgaaagca
     8221 gcgatgaaag cgaaagataa ggaatcttta caagtcatcc gaatgatcaa gtcatctata
     8281 caaaacgagc agattaaaga aggtcacgac ttgacagaag aagaagaact aacggtgttg
     8341 tctcgcgaaa tgaaacagcg acgtgattct ttgcatgagt ttgaagaagc aggacgtgac
     8401 gatttagctg aaaaagtaaa aagtgaaatt gtaatagtcg aaaaatatat gccggaacaa
     8461 ctttccgacg aagaaattcg acaacttgtt caagaagcaa tcactcaaac tggtgcttca
     8521 tcaatgaaag aatttggcaa agttatgggt gcaattatgc ctaaagtcaa aggaaaagca
     8581 gatggaaacc aagtgaatgc gattgtgaaa gaactattac aatcttaatt ttatcattta
     8641 aactgcggac agtgattgtc cgcagttttt tgtgttcgta aaaaaagaac taaaaaacag
     8701 ctagaagaaa gaataagatt ctttcttcta gctg
//
LOCUS       DAGCYL010000119         8629 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.121, whole genome shotgun sequence.
ACCESSION   DAGCYL010000119 DAGCYL010000000
VERSION     DAGCYL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.121"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            61..540
                     /locus_tag="MB216_002043"
     CDS             61..540
                     /locus_tag="MB216_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBT4526842.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            757..1848
                     /gene="phnW"
                     /locus_tag="MB216_002044"
     CDS             757..1848
                     /gene="phnW"
                     /locus_tag="MB216_002044"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBT4526843.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            1841..1969
                     /locus_tag="MB216_002045"
     CDS             1841..1969
                     /locus_tag="MB216_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBT4526844.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            1973..2518
                     /locus_tag="MB216_002046"
     CDS             1973..2518
                     /locus_tag="MB216_002046"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBT4526845.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(2972..4663)
                     /locus_tag="MB216_002047"
     CDS             complement(2972..4663)
                     /locus_tag="MB216_002047"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBT4526846.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(5083..6030)
                     /gene="arcC"
                     /locus_tag="MB216_002048"
     CDS             complement(5083..6030)
                     /gene="arcC"
                     /locus_tag="MB216_002048"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBT4526847.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(6145..7164)
                     /gene="argF"
                     /locus_tag="MB216_002049"
     CDS             complement(6145..7164)
                     /gene="argF"
                     /locus_tag="MB216_002049"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBT4526848.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(7255..8484)
                     /gene="arcA"
                     /locus_tag="MB216_002050"
     CDS             complement(7255..8484)
                     /gene="arcA"
                     /locus_tag="MB216_002050"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBT4526849.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2477 a         1748 c         1592 g         2812 t
ORIGIN      
        1 gggcaatcct tctttttttt gttatgatac atgtgaaatc tttcgtaaag gagaaagaaa
       61 atgcgcaaag ctcaaagaca tttgctgatt aaacaagtca ttgaagaatt cccgatccgt
      121 acgcaagaag aactattaaa caaacttggg cattatggcg ttaccgccac gcaggcaacg
      181 atttctcgag atatccgtga tctaaaaatc gtgaaggcac ctgatgaaaa tggcgtttcc
      241 cgcttcgtat tgtttcgcga aaaaggaact cctgagacta ggactgagga agaggatcgg
      301 ctggttcaaa tgattgaaga cattgtattg aaagtcgaac gagtccactt tttgacgatc
      361 gtcaatacct tgccagacaa tgctcattta tttgcatctg ttttagatga gatcaaacct
      421 ccctctattg tcagtacaat tgctggattc gatacgatca tggtcatctc ggaaaatgag
      481 gaggacgcaa aacgagtaga agaatttctt caccatccaa ctgaagtatc acttttttaa
      541 tcagagcacg tattcctaat atggaatgcg tgttttttta ttttcaatat tttccttatt
      601 gaataaacct ttacactctt tacaaaaata catcattttt tattcaaatt tttcaacacc
      661 ttgattgaat cacaataatc atcatttata ttggatgtta acaagagcag cttcttgctc
      721 tttgtgtatt tcaaaaaaga aatgaaagga aatcatatgt ataaattatt aacttctgga
      781 ccattgacga cttctgaaac agtgaaaaat gctatgctga tcgatcattg tacttgggat
      841 caagaataca aagaaattac acaatggata agaaaaaaat tattggatct tgctcaagta
      901 tcggaaggaa tatacacaac ggtcttaatg caaggtagca gctccttcgg ggtagaatcg
      961 gtattaagtt ctgtgatcag gccagaagat actgtactga tttgttcaaa cggcgcatat
     1021 agacaacgaa tgatcaacat gtgcgaatgt ctgaaactga actataccgt ttatgaggta
     1081 tcggaacatc aaatcctcca agctcatgtg attacacagc tgattgaaga gaatccgtct
     1141 atctccgcac tagcaatggt ccatagcgag actacttccg gtattttgaa taatttggaa
     1201 gagattgctg ctgtcacaca agaaagagat cttttgttta tagtggacgc gatgtcaagc
     1261 tttggtggga taccgatttg tgtagccgaa ctagggattg attttttgat ttccagctca
     1321 aacaaatgta ttcaaggtgt tccaggcttt tcttttgtac ttgctaataa agcggaactg
     1381 gaaaaacgaa aaaatgttag ccgaagtttg tcacttgatc tatatgacca gtaccgcgtg
     1441 atggaaaacg agaatggcaa atggcgtttc acttccccta cccatacggt tttggctttt
     1501 caacaagcgc tgaaggaact agaggcagaa ggaggaatcg aaactcgata ttcacgttat
     1561 ctcgccaata atcatgtgct acgacaaaaa atgacagaga tggggttcca gcctttcatt
     1621 caagaatatc aggggccatt catcacctct tttctttacc ctaaggactg gcgatttgat
     1681 tttaatcatt tttatcagta tttaaagcaa aatggatttg tgatctatcc cgggaaaatc
     1741 agcgatcatg aatgttttcg tatcggaaat attggcgaga tctatccgga agatattttg
     1801 gaactgacag caattatcaa agactataag gagaacgtta atgattaaaa ctgtaatttt
     1861 tgattgggca ggaacaactg tggactttgg ctgtatggct cctgtccacg cttttagaaa
     1921 tgcttttcta gaaaaaggaa tccaactgac tgacaaagaa atccgctaac cgatgggaaa
     1981 attaaaatgg gatcatatcc aacagcttct ttccctccct tctgtcaaaa aacaatggat
     2041 agctatttat ggacaactac ctcaggaaaa agaggtggag gaattgtatc aacaatttga
     2101 acattatctg tttaaagatt tggtcaagca ttctaccttg aaacccgaca cattggaaac
     2161 gatcgaacgg ttgagaaacg ctgggatcaa tattggttcc acgaatggat ttaatgctcc
     2221 tatgatgaca atcgtagcag aaaccgcaaa aaaagaaagg tattcaccag acttcatggc
     2281 aacttttgaa gacgtcgaag ggtatggtcg accgtttcct tatatgatcc ataaaaacat
     2341 gcagtacttc aaaaacaagt cggttgatga agtaatcaaa gtcggagata cggtctcaga
     2401 tatccaggaa gggaaaaatg ccggagtgtt gacagtaggt gtaattgaag gtagttcgct
     2461 aatggggatt tcttatgatg agtaccaaaa attagagatg gggaaaaaga tcgtttaaaa
     2521 cagtctgttg ctcttagatt ccaacaagcc ggcgctgata tttgtgtaaa caatttaaaa
     2581 gagttaacag aatacatttt gtacgctgac aagaatccag aatgactttt atacaaaata
     2641 agaggttgtg aaaaagctgt cctgtatcaa gtaataaaaa cagccaagca aaaatagctc
     2701 ctcatatttt tcgtttggtt aggcttatta ccgcagatgc aagcttttga acaccgttta
     2761 ttcgaaaaaa aaaagggagc tgtgacacga cttttgtcac agtcccctta tttgcaaaca
     2821 aacagtgttt aagagacaac ctctccacta aacggtgttc attcagctga ctctcggtat
     2881 ttgctcaaaa aacgataaaa tttgaaaagc tattttctct tttgtttctc taatactcgg
     2941 atcagaacgc tgatttcaca ttctcttttt cttacatctt atctggtgca tgaagtccta
     3001 gcaaacgaag gtcttctttc aataatacag tcactgcata aactagagct aagcgtgctt
     3061 ctttttgttc atcatcagct aaaatcttcg tatgagcata gtatttgttg aatgcttgtg
     3121 ctaatttgat tgcatgtttc gcaatcactg acggttcgta tttttcccca gcagacaaga
     3181 ccgtttcagg gtatttttga actaatttaa ccacttccca gctggtatcg tcattcaatg
     3241 catagtctgc tgtttctgaa gggacaaatc ctgctttttc caacaggctg actgcacgag
     3301 catgtgtata ttgcacataa ggtccagttt ctccttcaaa tcgaacaact tcttccaggt
     3361 taaagtcaaa tgtattcagt cgatcgtttt tcaaatcgtg gaaaatgact gccccaaccc
     3421 caacttgttt agctacagca tctttgtttt ccaaatcagg gtttttctcg ctgatttgtt
     3481 ctttagcaga ttggattgct tcatttaata cttcttctaa taagacgatc tttcctttac
     3541 gagttgataa ttttttacca ccttgcgtga tcaaaccaaa tggaatatgg tgcatgtcgt
     3601 cagaccagtc aaagcccatt tccttcaaga cagctttcaa ctgtttaaaa tgatagcttt
     3661 gttcattccc tactacatat agagattgtt tgaagtcgta agtacgttta cggtacaatg
     3721 cagcagctaa gtcacgtgta atgtatagtg tcgcaccgtc tgacttctta atcaacgctg
     3781 gattcaggtc ataagcagat aggtcaacga tctctgcacc tttatcctcg ttcagcaaat
     3841 gtttttcttc taaaagtttg acgatttcat ccatcttgtc attgtagaaa gcttcaccgt
     3901 tcaacgaatc gaaacgtact tccagcagat catagatttt attgaattct ttcatcgatt
     3961 catcacggaa ccattgccat aattgaatag cctctttatc gccttcttcc agacgtttga
     4021 accaagcacg agcttcttca tttaattctg gttctgtttc agctacttca tggaattgga
     4081 cgtataaacg aagcaattca ttgattggtt ctgctttaac agcttcttct gtaccccatt
     4141 ttttgtaagc gacgatcagt tttccaaact gtgttcccca gtcacctaaa tggttgatgc
     4201 ggattggctg ataaccgatt ttttccataa tgaaaccgat cgaattaccg ataactgtcg
     4261 aacgcaaatg tcccattgag attggtttgg caatattagg agaagacatg tcgattggta
     4321 ctgttccttg attcccgatg ttgctatccc cataatgttc tttttctttc actacggttt
     4381 gcaaaacttt tttgctgata agttctttat tcatgaaaaa gttcaagtaa ggtccgacaa
     4441 cttcgatttt ttcaaagttg gcagaatcga ttttttctgc taaatctgca gcgatttgct
     4501 gtggcgcttt tcggtaaact tttgctaatg aaaaggctgg aaatgcaaca tctccgtgtt
     4561 cagctgattt tggattttct agtaattgct cgacttgttc cagtgttaaa tcatctttga
     4621 cagcgtcata gacagctttt gcgacaagat ctttattatt catctttatt cctcctaaag
     4681 tgttcccttg attgttaaac aaaaaaaatc tctagcgacg gcatacttag tatgccatca
     4741 ctagagacga gatttcccgc ggtaccactc caattgtcct aaaaaaggac cagctccatc
     4801 tgatatcggt agacgaccgt ctgtttaaaa cagaattctc caagtgcgct tcgttgtatc
     4861 ttcttcattc ggcttccacc acttccgaat tcgctagcca agaatgatac aagtactctc
     4921 ttgttcaaca aatcttagtc ttattaaact ttcttgaatt atatcagaaa aaaaaacgaa
     4981 aggctagtcg tttcataaaa aaaaaatcca gatggcctaa aggagaagaa ataccacctg
     5041 gatagctata aatcatttcg taaatacgaa agacttaaca aattaatcag ctacgataat
     5101 cgttccttct tcagaagcta aaagattctc gatattttct aaagaagtga tgattgcctt
     5161 agcgtttggt tttgcttcaa caaatgcaat agctgcttct actttcggaa gcatgcttcc
     5221 tggagcgaat tgcttttcat caatgtattg tttcatttct gaaacagtaa cagtttctaa
     5281 tttcttttga tctggttttt ggtaattgac atacacattg tctactcctg tcaatacgat
     5341 cagcaaatcc gcatcgatga tttcggctaa tttttctgaa gcaaaatctt tatcgattac
     5401 ggcttctcgt ccttctaatc cagtagctgt ttctacaaca ggaatcccac cgccaccaac
     5461 agagactgtg ataactcctt gatcgaccag cgtttcgatg acacgtgctt ctttaatcga
     5521 gatgggttta ggggaagcga ctacttttct ccatccacgt ccagcatctt ctttgaatgt
     5581 cacagtggag tcagcattca tttgttcttt ggcttcttct tccgtgtaga atggaccgac
     5641 tggtttactt ggatttttga atgaaggatc attttcatcc acaataactt gagtgactaa
     5701 agaaaccaca tctttatcga tccctttttc cttcagtact tcgcccatgg cgttttgtaa
     5761 ccagtaaccg atagatcctt ctgtcattgc aacacatgta tctaaaggca ttgccggtgt
     5821 tttctcagaa tcagcagctt gctgctgaat caataggtta ccgacttgcg gaccatttcc
     5881 gtgggagatg atcaattcat ctccttgttc gataaatttc actaaatact ttgctgtttc
     5941 cattaacgct tcttgttggg cttttgcact tgcatccgta gacaagatgg cattgccccc
     6001 taaagcgaca actacttttc gtttggccat ttcattcgtc ctttcttcag ttctaccgaa
     6061 ttttctagat aacaaaacga gccagcgttt tgtgattttc cctcaatgct gactcgtttt
     6121 ttcgtgagat gaatgtggtt atgcttaaac gcgagggata aacaagttac ctaatgttgc
     6181 tgccatgatc gctttgattg agtgcatacg attttctgct tggtcaaatt gacgagcata
     6241 tttgctgcgg aagacttcat ctgttacttc catttcagtg atgccaaagc gttctttcat
     6301 ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc aagcagtgca agaagatcaa
     6361 acgatctgtg ttgtgtgttt tttctaccat ttccatattg atttggtaag gtttcaacaa
     6421 tttgatacgt tcttcgaatt tatcttcttc acccattgat acccatacat cagaataaag
     6481 gacgtctgct ccgtctacac ctttatcaac atcgtcggta atcatcaatt gtgcaccgga
     6541 tttttcagca aagccttctg ccattttgac gatttcttct tcaggttgta attcttttgg
     6601 tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg actaataaag agttagccat
     6661 attgtttcga ccatctccgc agtaagcaac agtgatacct tctactgttc caaagttttc
     6721 ttggatagtt aaaaagtcag cgatcatttg tgttggatgc cattcatcag tcaagccatt
     6781 ccaaactgga acaccagaga attctgctag ttcctctacc attttttgac tgaatccacg
     6841 gaattcgatt ccgtcaaaca tacgtcctag tactttagct gtatcttctg tggattcttt
     6901 tttacctaat tggatatcat ttgcacctag atattcagga tgtgcaccta gatcgatcgc
     6961 tgcagtagta aatgcagaac gagtacgtgt agatgttttt tcaaataaaa gcgcaatatt
     7021 ttttccttct agatagtgat gtgggatacc acgttttttc aagtctttca agtgttctga
     7081 aaaatcgatc aaatattgta attcttcttt tgtaaagtct ttttctgcta ataagcttct
     7141 tccttggaat actgattctt tcatattaaa cattctcctt tagattgttt ttatttaaga
     7201 ttaaagagga aatcccgacg gcagctgaca gtaagactgc cgtcaggaaa caaattactt
     7261 caagtcttca cggacaagtg gttggctcat gcagcgtgga ccaccacgac ctcgtgacaa
     7321 ttcacttgaa ttgatttcta acactttgat gccatagcta cgaagcaatt cgttcgatac
     7381 ataatttcgg ttataagtga ctacaacacc tggagcgatt gccaatgtgt ttgatccatc
     7441 attccattgt tctcgaggtg caacgatctc atctccatta ccagttggga tcagtacaag
     7501 atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag ttatctgaat gagtaatctt
     7561 gatatcatca ccatctggtt cgattgtaaa gacatccatc ttaccgtctt tactttgaat
     7621 tgctggatgg atagtgaatt tatcgtgatc taccattgta aatacggtat ccaaatgcat
     7681 cattgcacgg ttgttcggaa ttttgattgc caacactttt tcaaatcctg aattttttgc
     7741 gaataggtgg cgtgctaatt tttctaaagc tttcgcatta gttcgttgag aaatcccaac
     7801 agctactact ttatcgctta agatcaattc gtctccgcct tcgatatgat ctggattttc
     7861 acgatccaac catacttcga ttcctttatt tgcaaatctt ggatgatgtt tcaaaatata
     7921 ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc atcttattga ctgtcatccc
     7981 atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag tttggcattg ggtccatgta
     8041 aaatggatag tcatcgtctg ctgacagatc atatagactg ctagaacgta cttcgatatc
     8101 ttttgtacga accccagcca tgattttatc taccatttct tgcgtttcca ttgatagaag
     8161 gaactcatga agaccctctc ttacagcatt cgatgcaata tgagattcat ccaacatttt
     8221 attcaagaat tgttctttga cattgcctgc gtcaatcgct tctgccgcta atttttccaa
     8281 gtaaagagtc tcgacgcctt cattttgcaa tgtcttcgca aagttgtcat gttcttcttg
     8341 agcaatcgga agatatggaa tatcatcgaa caacagacga tccataatat ctggtgtcaa
     8401 attttccact tcttgtccag gccgtttcag taaaactgtc tttaatttcc ctatttcaga
     8461 gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt ctttttgatt acatgtatag
     8521 gatatcctcc ttttgtaatc actttcataa ttttgtctca ttcaaaatga gacattcctc
     8581 acattaatat tcgataatta aataattaaa cgtttttcta tttttaagc
//
LOCUS       DAGCYL010000120         8577 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.122, whole genome shotgun sequence.
ACCESSION   DAGCYL010000120 DAGCYL010000000
VERSION     DAGCYL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.122"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(8..2389)
                     /locus_tag="MB216_002051"
     CDS             complement(8..2389)
                     /locus_tag="MB216_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBT4526850.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2379..3026)
                     /gene="recU"
                     /locus_tag="MB216_002052"
     CDS             complement(2379..3026)
                     /gene="recU"
                     /locus_tag="MB216_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBT4526851.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3090..3629
                     /locus_tag="MB216_002053"
     CDS             3090..3629
                     /locus_tag="MB216_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBT4526852.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWDEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3719..4138
                     /gene="gpsB"
                     /locus_tag="MB216_002054"
     CDS             3719..4138
                     /gene="gpsB"
                     /locus_tag="MB216_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBT4526853.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4160..4527
                     /gene="rnpB"
                     /locus_tag="MB216_002055"
     ncRNA           4160..4527
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="MB216_002055"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4736..5902
                     /locus_tag="MB216_002056"
     CDS             4736..5902
                     /locus_tag="MB216_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBT4526854.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5948..7444
                     /locus_tag="MB216_002057"
     CDS             5948..7444
                     /locus_tag="MB216_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBT4526855.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMEDLYFNVYQVK"
     gene            7525..8208
                     /locus_tag="MB216_002058"
     CDS             7525..8208
                     /locus_tag="MB216_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4526856.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2693 a         1559 c         1823 g         2502 t
ORIGIN      
        1 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
       61 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      121 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      181 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      241 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      301 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      361 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      421 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      481 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      541 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      601 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      661 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      721 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
      781 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
      841 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
      901 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
      961 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1021 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1081 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1141 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1201 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1261 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1321 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1381 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1441 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1501 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1561 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1621 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1681 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1741 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     1801 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     1861 gtttcagggt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     1921 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     1981 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2041 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2101 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2161 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2221 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2281 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2341 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2401 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2461 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2521 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2581 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2641 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2701 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     2761 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     2821 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     2881 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     2941 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3001 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3061 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3121 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3181 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3241 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3301 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactggg
     3361 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3421 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3481 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3541 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3601 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3661 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3721 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     3781 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaaac
     3841 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     3901 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     3961 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4021 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4081 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4141 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4201 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4261 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4321 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4381 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4441 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4501 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4561 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4621 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4681 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4741 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     4801 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     4861 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     4921 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     4981 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5041 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5101 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5161 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5221 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5281 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5341 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5401 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5461 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5521 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5581 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5641 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5701 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     5761 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     5821 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     5881 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     5941 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6001 agctttagga atccttgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6061 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6121 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6181 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6241 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6301 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6361 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6421 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6481 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6541 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6601 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6661 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6721 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     6781 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     6841 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     6901 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     6961 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7021 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7081 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7141 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7201 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7261 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7321 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7381 taatccgcaa tacctgatcg actatatgga agacctgtat tttaatgtat atcaggtaaa
     7441 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7501 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7561 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7621 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7681 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7741 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     7801 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     7861 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     7921 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     7981 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8041 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8101 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8161 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8221 cagaagtggt cagctccaag atatttcgga tcattttccg taaaatttac ttctggaaca
     8281 ctgtttacca agaggtcatg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8341 aaaatagctt ctcatgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8401 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8461 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaata
     8521 aagatggaag ttttatgtat ttttattggt ctatgcaata aataaattaa atattca
//
LOCUS       DAGCYL010000121         8492 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.123, whole genome shotgun sequence.
ACCESSION   DAGCYL010000121 DAGCYL010000000
VERSION     DAGCYL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.123"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            4..2376
                     /locus_tag="MB216_002059"
     CDS             4..2376
                     /locus_tag="MB216_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526857.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAESGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVTKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFSLGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="MB216_002060"
     CDS             2437..3897
                     /locus_tag="MB216_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBT4526858.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERSNGTYDFDRFYQGKGDINAQILFSYKAEQYYKGYKKHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="MB216_002061"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="MB216_002061"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBT4526859.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSLLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKSQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYHKAAACTSEACDFRLWTTIAKKKLTATQLKEII
                     QNGRTSQPVKGLKGQKGSFEATIVLKEDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="MB216_002062"
     CDS             6128..6277
                     /locus_tag="MB216_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526860.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6358..6954
                     /locus_tag="MB216_002063"
     CDS             6358..6954
                     /locus_tag="MB216_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBT4526861.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            6967..7866
                     /locus_tag="MB216_002064"
     CDS             6967..7866
                     /locus_tag="MB216_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBT4526862.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            7869..8354
                     /gene="ssb"
                     /locus_tag="MB216_002065"
     CDS             7869..8354
                     /gene="ssb"
                     /locus_tag="MB216_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBT4526863.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
BASE COUNT         3202 a         1445 c         1814 g         2031 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgca
      661 ccaactgatt ctctgaagga gaaagaaaaa gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agagttgatc
      781 gatcaactta aaacgttgga taaccaatac gctgcagaat caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgttacga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgag
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcatgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatt gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacaa ttgaagcctg aagactatcc gaaaatcttc
     1501 ccgaaccgcg tctttgattt tcaattggat aaaacagggc agacagaact tcaagttggg
     1561 attcaagcgg tagccaaaaa tattggaatt caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtacgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaattttc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgaaaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac tctaagcaac aatttaaagc agccacagat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaatg agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtttcgat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagtcattta tgaagaagtc gcaaaagaag aaaagatcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaatt ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagcttgtga acgctcgaat
     3661 ggtacgtacg attttgatcg tttttatcaa ggaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa agcatgaaca tgaatttgta
     3781 cctgaagtga aaaaggattg gaacgatcaa ttgaagcatg agatccagca acaagaaatt
     3841 agaaagcaaa aacgtgcgat gttgtttcaa caaggcagac aacaagaaag ggagtgagat
     3901 cgtcacgatg aaaactgtca ttttagcaga aaaaccgtca caagcgaagg cttatgccga
     3961 tagcttttct aaagccaccc gaaaagacgg gtattttgag atccaagatc gattgtttcc
     4021 tggtgaaact gtgattacgt atggttttgg tcatttagtc gagttagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacattta cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaac gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgc gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagcag gcgccgatca tggaaagata tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagatg cagaagagac
     4381 ctacccaaaa tttgaagaag cacaagcacg ccagatcgcc gattggttga tcggcatgaa
     4441 cggtagccta ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gtttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttacaggaaa agattagaaa
     4561 cttccaaaaa gaaccatatt ttgaaggaaa atcccaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggtacg atccaccaag ttgaaaccga
     4741 gaaaaaaagt gcggcaagtc cacgtctttt ttctctatcc agccttcaat ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagatac tttggaagcc atgcaagggt tgtatgaagg
     4861 aaaatatcta agctatccgc gaacggatac cccttatatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttaaaa gccgaagcga ttccgactcc
     4981 tattcataca ccgaatagtc ggtatgtgaa taataagaaa gtccaggaac attacgcgat
     5041 cattcctaca aaaacggtta tgacagcagc ggcttttgaa caactaagtc cactgcagca
     5101 ggctatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa agtataccta
     5161 tgaagaaaca acgatcttga cgcaagttca gcagcttcag ctaaagacag tcggaaaagt
     5221 accagtagat ctaggttgga aaaagctgtt tggcaaagag agcgacagaa aagaaaagga
     5281 agaagaaccg ctacttccta aagtgacaaa aggagaagcg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaacgaagc caccacaacc ttataccgaa ggtacgttga tcacagcgat
     5401 gaaaacagct ggaaaaaccg tggatagcga agaagcacaa tccatattga aagaagtaga
     5461 aggaatcggt acagaagcaa cacgagcaaa tattattgaa actttgaaac aaaaggaata
     5521 tatcaaggta gaaaaaaata agcttgtggt gacaaataaa gggattttac tttgccaagc
     5581 cgtggaaaaa gagccgctgc taacaagtgc cgaaatgacc gccaaatggg aaagttactt
     5641 attaaagatt ggtgagcaga aaggaacaca agctactttc ttagctaata tccaaaaatt
     5701 tgtctctcac ttgttggaag tcgtaccagg acaaatccaa tccactgatt ttggatcgac
     5761 gttacaagag gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt tagggatttg
     5821 tccaaaatgc caggaacaag aagttttact ctatcataag gcggccgcat gtacgtctga
     5881 agcttgtgat tttagattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggaaatc attcaaaatg gccgaacaag ccagccagtg aaaggattaa aaggtcaaaa
     6001 gggttcgttt gaagcaacga ttgttctaaa agaagacttc acaacgagct ttgaattttc
     6061 tgaaaagaaa aaaacgaact ataagaaaag aacacgccgc actacaaaat aatggagggg
     6121 ttatcttatg cgaaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagcaaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa ttgaattaga tcgataaaat cttcctggct attgtatacg
     6301 cataaactta tgttatacta tatttataag tttatacgta tacaaaggag ggagaaaatg
     6361 acaaaagcaa tggtttacaa gctgctagaa gaatttaacg gaactttggc tttgaaggat
     6421 gccaaaaaag cgggcatttc tcctgtaacg atcaaacgta tggtagatcg tggagagctg
     6481 gatcgtgagt atccaggttt tttcacctta cctggtcaat ttcctgatga attgtttatg
     6541 gcacaaaaga aatatgagcg cggcattatt tcgcatatca ccgcgttgga tttatatgat
     6601 ttaacggata tgatccctag acagatcgat ctaaccgtac cttacggtta tcacgtttct
     6661 gaaaaggggt tgaaagaatt cgcagttgag ttacactatt caaaagcgga atggtatgaa
     6721 ttaggtaaga tcgaaataaa gagtcgttat gggaatcctg ttatagctta tgatccagaa
     6781 agaaccttgt gtgacatttg gaatccctgg tacaacgttg aggatgaaat caaagtaaaa
     6841 gcaatcaaaa actacatgga gtcggacaga aaaaatctta gaaaattgaa tgagtatcga
     6901 cgaattttgc ctacagacaa aacgatacgc tcttatatta tggctttaaa ttaaaggaga
     6961 agacacatga cacctacgca attaaaagca aaagtgaaga atatttccag agaaaaagaa
     7021 gttgatccgc agcttgtttt aagacatttt atgatggaaa aattcttgga aaaaatctca
     7081 gagtctcctt atcgtgaaaa ttttgtgtta aaaggcggct tcttaattgg ctcaaagtat
     7141 ggcattgaaa atcgaacaac aaaagacatt gatacgactt tacgggaaat gaaagtaacc
     7201 aaagaaacat tgacaacggt tctaaacgat attttttcaa cgccgacgaa agaaggcatt
     7261 caatttgaga ttcaaggaat gaaagaaaca agggaagctg attattatcc agggtttagc
     7321 ttacgtgtac tggcacattt ggaaaacatg cggccagatt ttaaggtaga cgtgacgaca
     7381 ggtgactcga tctatcctgc aacgatcact cacagtcata aattgatgtt tgaaaatcga
     7441 acgattcctt tggaatctta tcctacagaa caaattattg ccgaaaaatt aagtgcaacc
     7501 tttgattttt taacagataa ttcgagagga aaagattttt atgatctcta tactattcct
     7561 aaaatggaaa agatcgatga gaagaccttg aaagatagtg tgagaaatac atttacacgt
     7621 cgaggaaaaa ctgatccgtt aaagatttat tacgaaaagg atatggatgt actgaaaaat
     7681 agtcatgagc taaaagatac ctggaagaaa tatcaaaaaa gcaatcctta tgctgcgtcg
     7741 atcacgtacg aagagacaat ggattcgatt agtaatttga tggaaaaagt gattcacgtc
     7801 gaagaaaaag agcgaaaaat agcttatcaa agacaccaac agatgaacag agaaatggag
     7861 agataaaaat gatcaataat gtaactttag taggacggtt aaccaaagat ccagatttac
     7921 gttataccgc aagtggcaca gcagtggcta cttttaccct agccgttaat cgtaatttca
     7981 cgaatcaaaa cggcaatcga gaagctgatt ttatcaattg cgtgatttgg cgcaaaccag
     8041 cagaaacgat ggcgacatta gctaaaaaag gcatcttgat cggcgttgtc ggacggattc
     8101 aaacacggac ttatgacaac cagcaaggac aacgtgtgta tgtgacagaa gttgtagctg
     8161 ataacttcca actattagaa agcaaagcag cgacagagag ccgtgcgcac gctgatcaat
     8221 cgtccacgag tccaagcacc accacttttg aacaacggga tacagcgacg cctaacaaca
     8281 atggattgaa cgcttcacag aatccatttg gtggacaatc aatcgatatt agcgatgatg
     8341 atctaccgtt ttaagtagca ttttctattg gttgcatttg ctacggttta tgttataatt
     8401 tagtcaacaa aaagaatttt caatctataa ccaaaaacca ctagctggcg ggctagtggt
     8461 tttcttttac gtttctatta taagaattaa cg
//
LOCUS       DAGCYL010000122         8489 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.124, whole genome shotgun sequence.
ACCESSION   DAGCYL010000122 DAGCYL010000000
VERSION     DAGCYL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.124"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            115..2724
                     /gene="clpB"
                     /locus_tag="MB216_002066"
     CDS             115..2724
                     /gene="clpB"
                     /locus_tag="MB216_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBT4526864.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2875..3561)
                     /locus_tag="MB216_002067"
     CDS             complement(2875..3561)
                     /locus_tag="MB216_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526865.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3539..4033)
                     /locus_tag="MB216_002068"
     CDS             complement(3539..4033)
                     /locus_tag="MB216_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBT4526866.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4053..5273)
                     /locus_tag="MB216_002069"
     CDS             complement(4053..5273)
                     /locus_tag="MB216_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4526867.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5420..7255
                     /gene="lepA"
                     /locus_tag="MB216_002070"
     CDS             5420..7255
                     /gene="lepA"
                     /locus_tag="MB216_002070"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBT4526868.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(7349..8476)
                     /locus_tag="MB216_002071"
     CDS             complement(7349..8476)
                     /locus_tag="MB216_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4526869.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         2732 a         1624 c         1704 g         2429 t
ORIGIN      
        1 aaagtcaaaa aactatgtgt atattttgac caaaattgac caaagcgata taatagtttt
       61 tgtaaagatc aaagaaataa gtgatcttac ctttaattag gagggtatac ctgtatgaat
      121 attgagaaaa tgactacgac gcttcaagaa gcgattgctg aagctcaaca gattgcggtc
      181 actcgaaaac atcaagatat cgacattgcc catgtatgga aaatcttttt acaatctaat
      241 cattttggtc gaaatttcta tacggatgcc ggattggatg tcgaatcttt tgaacatgaa
      301 attgatcgtc tactggatga gtatccagtt gtttctggtg gaaacgtcca atatggacaa
      361 aacctcagcc agaatttctt tcgattattg aatgaagcag accaaatacg tgaaagcttc
      421 ggcgacgagt ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt aaaaaattat
      481 ccgttaactg tttatttgaa taaaaacgga ttatctgaaa aagaattgcg taaaaacatc
      541 gaagagatga gaggaggaga aagagtgacc tcacaaaacc aagaagaaca atacaaagca
      601 ttagaaaaat acggcgttga tttagtacag caagtcaaaa gcggaaaaat ggatccaatc
      661 atcggtcgtg atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa aacaaaaaat
      721 aatcctgttc tgataggaga acccggtgta gggaaaacgg caatcgttga agggcttgct
      781 caacgtatcg tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat tttttcatta
      841 gatatgggtg cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga aagactaaaa
      901 gcagtcttga aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat cgatgaaatc
      961 cacaatatcg taggggcagg aaaaacagaa ggaagtatgg atgccggaaa tctgttgaaa
     1021 ccaatgcttg ctcgcggtga attacatttg atcggtgcca caactttaga tgagtaccgt
     1081 caatatatgg aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt agtcaaagaa
     1141 ccaacagtgg aagatacgat cagtattctg cgtggattaa aagaacgttt tgagatccac
     1201 catggtgtta acatccatga caacgcatta gtagcagcag ctactttatc tgaccgttat
     1261 attactgatc ggttcttacc ggacaaagca atcgatttga tcgatgaagc cagtgcaacg
     1321 attcgggtag aaatgaactc tatgccaacg gaattagatc aagttactag acgtttgatg
     1381 cagttggaaa tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag taaaaaacgc
     1441 ttgaaaaatt tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc gatgaagatg
     1501 cagtgggaaa cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc tgaaatcgat
     1561 aaagcaaaac acgaattaga agatgcagaa aataactatg atttagaacg cgctgctgta
     1621 ttgcgtcatg gtacgatacc tcaattagaa aaagaattga aggaattaga agctaaagca
     1681 aaagacagcg agattaagat ggttcaagag tccgttaccg aaaatgagat tgctcaagtc
     1741 gttggcagat taacgggtat ccccgtaacg aaacttgtag aaggtgaacg agaaaaacta
     1801 attaaattga atgaaacact tcataaacga gtgattggtc aagatgaagc agttgatgcg
     1861 gtcagtgacg cggtaattcg ttcacgggct ggattgcaag acccaaatcg tcctctaggt
     1921 tctttccttt tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa agcgttagct
     1981 gagaacttgt ttgactcaga agatcatatg gtacggatcg atatgagtga atatatggaa
     2041 aaacatgctg tgtctcgtct agtcggagct cctccaggct atgtaggtta cgaagaaggt
     2101 ggtcaattaa cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt ggatgaaatc
     2161 gaaaaagcgc atcccgatgt cttcaatatc ttgttacaag tcctagatga cggacgcttg
     2221 actgattcaa aagggcgagt agtagatttc aaaaatacgg ttctgatcat gaccagcaat
     2281 atcggttcac agttactgct ggaaggcgtg actgctgatg gaacgattcc agaagcagtg
     2341 gctgaacaag tcaacacgct attacgtgga aacttcaaac cagaattctt gaaccgaatc
     2401 gacgacacga ttttatttac accattgagc ctagacaacg tgaaaggaat cgttgacaaa
     2461 atggtggcgc aattggcaca acgtctggaa catcaagaaa tcctgctgac aatcagtgat
     2521 gaagccaaaa cgtggattgc tgaaaatgct tatgaaccag catatggtgc aagaccattg
     2581 aaacgattta tcactaagga agtagaaaca ccactagcta aagaaatcgt tgcaggacat
     2641 gttatgccta aatcaaaagt aacgattact ttacttgatg gacaacttca tttcaaaaca
     2701 gaagagctag aagaaatcgt ctaatcttcc attgagacta taaaagaaaa aaggggtctg
     2761 acacaactag tgtcagacct ccttttttca aataaacagt gttccagaag tagtttcttc
     2821 gaaaaataag ccgaaatttc ttgaagctgc ccgcttctgt cacaacctct tttattaggt
     2881 atttttcact ttttctaatt tataactttc aaagtaacgc tcatcttttt tactttgaga
     2941 ccatgcaaac aatcccatta aggcagcacc tatcgtattt gtcagcaagt cgcccatcgt
     3001 atccatcaat gctgctcgtc cgacaaacag tgtgccatca gaagcagcaa aacgttgaag
     3061 attcatgcct aagaactggt cgcataagaa ttcccagaac tcccaaaaca cgccgcaaag
     3121 tccagcaaac gcaaagccga ataagaggaa caaccaagga ctggtttttg atatctcagc
     3181 atctttcatc aagtaaccaa tcaatccata gccaagagct gtcaggacca ttggactcac
     3241 cgcatgtaag attttgtccc aaaaagagat gatactgatc aaatgcatcc ctgtgcctaa
     3301 aaagacagag atgaacaaga aaaaccagta aaacaaaacg attgctgctg gtattttaag
     3361 tttgaaaact ttcaaaatga cttgtggcat gaaaatcaat gcaatcccta ataaagattc
     3421 cacgataaat gtgattcctt gagaagtcga atatttttgt gtgataaatt cataaaaatt
     3481 atagcagaca acgaatacac caaaagcaag taaagctatt tgcacctttt tgatttcttt
     3541 aatcactgaa agtccctcca tctaaaaatc tttttacttc tgaaagcaac tcaatttctg
     3601 aagaaaccac ttttccgttc aatttgatca aacctattgt atataagttc aggtagtgaa
     3661 attgattttc agctgtttct tgtagtgcag cgattttttt cgggtgggtt ccgccttgct
     3721 gtcgagtatc agtatataag ccgatcactg ggatgccttt cgcataagcc acaccgattt
     3781 ccgatgctac cccggcatcg atcgtcagcc catctagtaa agcaatcatt aaatcactag
     3841 ccaacacttt ttctgtgtca gcaagagcaa tcattcgact gtctgcataa gcagttttgt
     3901 cattgattgc ttcattttct tgaggaagat agatcgaaag ctcagaagag agctctctga
     3961 tttctttaac taactgttgg ttatataaca aatcactttt agcaaacatt ggtgcggcaa
     4021 aataaatttt catctaaaca tcatcctttt tatcaaaaat aatcgctaaa gtaagcttcc
     4081 atgtttggaa tgatacgctc tagtgacttc agtgtttctt catctgtttc taaccgttcg
     4141 attcttgcca aataagaagc acggcgataa ttcatcatca agcaagtcaa tgtttggata
     4201 tccagtcgaa cggattttcc tataggttca tttaagacac ggacttgtcc ctgttcatcc
     4261 catgttaaag cgaagacacc attattccaa ggagcgacag gatcttcaat gatgaaatga
     4321 aaagcatcag ctgttccaac aaacggaaac cgttgaagga attcttttac atcaacgatc
     4381 cgagccataa aatatggttc gatctgctct ttgatctgac tatcttcaat caaaaatgct
     4441 aaaggctcat ttttataaat atctccatgt acccagtaga tcatggaaaa atgtgcggaa
     4501 ataaagttcc ataagccatt tcgagcttct tgatcaagat agaacatttc ttttacgtga
     4561 aatatttctt cagctaccca gtagaacaga acacctcttg gttcatggtt gctatcgtag
     4621 tagacagcgg ctgtgcgctc atcttcattt tcgaaacgcc aatactcttc ccaatgaaaa
     4681 tcactgcgga ataaagcacc atggttttgt tgggcaaatt tgtcataaac ggaaaatacg
     4741 tctggatgat caactggtaa tcgctcaacc ataccactta ctgggatttg tttaggtagc
     4801 tgtgtatctc tgattttaaa ggaaagttta tcagacatga tttcccatcc tttacgccga
     4861 tagtagggga tactgtaagg atacaaataa gagatccatt gcttattctt gcgcatatta
     4921 tccaatgcaa cgataattag atcttgcatc aatccatggt tagcatattc tggataagtt
     4981 ccaactccag tcacaccacc cattttataa cgtgcaccgt gaatattgac ttcacaagga
     5041 tagatagcga tttgagagat cagtttattt tcgtggaacc atccgaacac tttcgatact
     5101 tccaaaatcg gttttttgga acggataaaa gctcgtttat tctcaaaccc gctttcctcg
     5161 atgtctgctt cagtcacttg gaacacatag gaaagcaatt cattgaattg atcgatatgt
     5221 tcttgagtga ccggttttaa ttcaagattt tctctaaaat ctttttgatc cattttttca
     5281 tctccattta ttagcttgat aacagtatag caaaattcta caaaaaaaac ttctatagct
     5341 gactaattaa aaactcttta aattactgct tttcgttggt ataatggaaa gtgctgatta
     5401 tatgaaaaga ggagacgcta tggatataaa gaaaatgaaa gaacgccaag agcagattcg
     5461 taatttttcg atcatcgctc atatcgacca tgggaaatct acattggccg atcgtatttt
     5521 agaaatgacg cacactgtga cgtctcgcga aatgcaagat cagctgttag actcgatgga
     5581 tttggaacgt gaacgtggaa tcacgattaa attaaatgct gtagaactca attacacagc
     5641 gaaaaatgga gaaacatata tctttcactt gattgacacc ccagggcacg tcgacttcac
     5701 ctatgaagtc tctcgaagcc ttgctgcctg tgagggtgcc gttttagtgg tcgatgcagc
     5761 acaaggaatc gaagcacaaa cattggccaa tgtttattta gcgttagaca atgatttgga
     5821 gatcctgcca gtcatcaata aaatcgacct acccgcagca gatcccgaac gtgtccgtca
     5881 agaaatcgaa gacgtaatcg gaatcgatgc tagtgaagct gtattagcca gtgccaaatc
     5941 agggattggg attgaagata ttctcgagca gatcgtggaa tatgttcctg caccaagtgg
     6001 agacttagat gcaccgctaa aagctttgat ctttgactct atttatgata gttaccgcgg
     6061 tgttgttcta aatgtccgga tcacggacgg tatggtcaaa cccggagaca aaatcaaact
     6121 gatgagtaat ggcaaaacat ttgatgttac agaggtcggg gtcttctcgc caaaagcagt
     6181 tgctagagac tttttgatgg tcggtgatgt cggttatatc acagcaagta tcaaaactgt
     6241 ccaagataca cgagtagggg atacagtaac gttagcagac aatcctgccg ctgaagcttt
     6301 accaggttac cgaaaaatga atccgatggt ttattgtggt ctttatccaa ttgatacttc
     6361 tcgttacaac gatttgcggg aagctttaga aaaactgcaa ttgaatgatg ctgctttaca
     6421 attcgaacca gaaacctctc aagcattagg ttttgggttc cgctgtggat tcctcggact
     6481 tcttcatatg gatgtcgtcc aagaacgctt ggaacgggaa ttcaatctag aattgatcac
     6541 gactgcacca tccgttattt accatgttaa taaaacggat ggtacgactg ctacagtaga
     6601 taacccggca gattttcctg aacctgtaac gattcaggat gtagaagaac cattcgtcaa
     6661 agcacagatc atggtcccaa atgattttgt cggtgcagta atggaacttt ctcaacgaaa
     6721 acggggagaa ttcattacga tggattattt agatgattac cgagtaaatg tcgtctataa
     6781 tattccgctt tctgaaattg tctttgactt tttcgacaaa ttgaaatcaa gcacgaaagg
     6841 ctatgcctca ttggattatg aaatgtctgg ttatcaaaaa agcaagttag tgaagatgga
     6901 catcttgctg aatggagaaa aagtcgatgc attaagcttt atcgttcacc gagactttgc
     6961 ctatgaacgt ggaaaagcaa tcgttgaaaa actgaaaaaa ctaatcccac gccaacaatt
     7021 tgaggtgcca atccaagcag ctattggaca aaaaatcgtt gcccgttcag acatcaaagc
     7081 cttgcgtaaa aacgtcttgg ctaaatgtta tggtggagat gtttctcgta aacgtaaact
     7141 cttagagaaa caaaaagaag ggaagaagcg gatgaaacaa atcggctccg tcgaagttcc
     7201 gcaagaagcc ttcatggccg ttctgaaaat ggacgaggac gaaccgaaga aatagcaagg
     7261 gtttgtttga aaacagtgta aaaaataaac catgtttaca tggatttgcc accggtttgc
     7321 caccggtggt ttttttcggt ggcaaaaatc aagaattctt tctaagattt tccatgatat
     7381 cgaccgtttc atttctcatc ttatctgtta catgcgagta agtatccatc gtaattgaaa
     7441 ttctgctgtg gcccaatcgt tcagagattt ctttcatttt tgcgccattt tcgagaagaa
     7501 gtgtagcatg agtatgtctg agagaatgga agttaaaaga gagggagagt gcattcgata
     7561 tcctcctagt attccatttc actacacttg gcgtaactag ctcaccgtct tccttcgtac
     7621 atactgcatt tgaatcaatg tagagctttc catacttcat tcgattttct aattgttgtt
     7681 tcttatgttt tttcagaatt gctagcaagg tttgtccaat aaaaatcgtt cgattggaac
     7741 tgcttgtctt tggcgtacca tatacccatg cgccgtcatt ctttaccatt tgtttctcta
     7801 cagtaattgt tccatttgaa aaatcgacat catcccacgt cagaccacaa acttcgccaa
     7861 cgcgcattcc cgtataaaat ccaatattca aaggaatata gaaaggatgt ccttcaggag
     7921 taatttctag catatgatca aagtcctcaa gagaaatgat ttttagatct tttttagtcg
     7981 ttggtcgtgc ttcatatttt ggtattttta catacagcat aggattttgc ttgattaatc
     8041 cccaaggata aaccgccata ttcagcgcat tcttaaggac agagtgagta atagtcattg
     8101 ttttcttcga gtaacccttt ttaaattcag cattgatgaa attttgtaaa agagcagggg
     8161 agagatccgt aagttttttc tttcctaaat aaccgtttat atgatttttg atggtaaatc
     8221 ggtagttttc ataggtattg tattttagat ttagtttaac gtattcctcc atccaaaaat
     8281 caaggtattg ttttactcga gtatccgtac ctaaaaagta ttgtcctgtt tcgtcaatat
     8341 ctgataaaac ttttcgtaaa gcagcttcgg cctctggtcg ggtgtctccg ccaactttct
     8401 ccactttttt tcttgagcca tcatcattga tatcttcgcc gtttgtataa ttaagcgaga
     8461 caaataaaaa agccatttat gttacactc
//
LOCUS       DAGCYL010000123         8297 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.125, whole genome shotgun sequence.
ACCESSION   DAGCYL010000123 DAGCYL010000000
VERSION     DAGCYL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.125"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            476..1339
                     /locus_tag="MB216_002072"
     CDS             476..1339
                     /locus_tag="MB216_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBT4526870.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="MB216_002073"
     CDS             complement(1468..3090)
                     /locus_tag="MB216_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4526871.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="MB216_002074"
     CDS             3339..3860
                     /locus_tag="MB216_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBT4526872.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="MB216_002075"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="MB216_002075"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBT4526873.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="MB216_002076"
     CDS             5941..6759
                     /locus_tag="MB216_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBT4526874.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="MB216_002077"
     CDS             complement(6862..7059)
                     /locus_tag="MB216_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526875.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="MB216_002078"
     CDS             7228..7806
                     /locus_tag="MB216_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBT4526876.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="MB216_002079"
     CDS             7901..8038
                     /locus_tag="MB216_002079"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526877.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2498 a         1488 c         1635 g         2676 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagt
//
LOCUS       DAGCYL010000124         8278 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.126, whole genome shotgun sequence.
ACCESSION   DAGCYL010000124 DAGCYL010000000
VERSION     DAGCYL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.126"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            628..2721
                     /locus_tag="MB216_002080"
     CDS             628..2721
                     /locus_tag="MB216_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBT4526878.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            2881..3747
                     /locus_tag="MB216_002081"
     CDS             2881..3747
                     /locus_tag="MB216_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4526879.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(3769..4206)
                     /gene="ndk"
                     /locus_tag="MB216_002082"
     CDS             complement(3769..4206)
                     /gene="ndk"
                     /locus_tag="MB216_002082"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBT4526880.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            4865..7543
                     /gene="mgtA"
                     /locus_tag="MB216_002083"
     CDS             4865..7543
                     /gene="mgtA"
                     /locus_tag="MB216_002083"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBT4526881.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            7630..>8278
                     /locus_tag="MB216_002084"
     CDS             7630..>8278
                     /locus_tag="MB216_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4526882.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEM"
BASE COUNT         2695 a         1421 c         1748 g         2414 t
ORIGIN      
        1 tatcaagagg tcttgagcct gccgtttaac tctgagccac aaggagcaat ttcaaaaaat
       61 agctccttat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
      121 gtttaccaag aggttgtaaa attagcgtcc tgacataatc taataccgag ggtcagctga
      181 ttgaacaccg tgtatcaaga ggtcttgagc ctgccgttta actctgagcc acaaggaaca
      241 atttcaaaaa acagcttctc atgttttata aatattgtga cttgtggccg aggagttggc
      301 aggagaacac cgtttaagga agaagttgtg aaaaagctgt cctgcatcaa gaaataagga
      361 gctgcgacaa gaattttgtc acagcctctt tttttgtacg aataccgaga aactggcttt
      421 tgaataccgt ggagtgaagc ggtcaccaca agacttttat ctcttcatta gtttaatcgc
      481 ttacatcgca gcggttttat gctgtgaaaa gtggacggaa taatctttag aaagaataaa
      541 aaaatcttaa gataacaact tttttaggaa attttcattt ttcaggagca aaataagaaa
      601 aaatgtgaat agagagaagt gatatacgtg acacaactta aaaaattgtg ccaaaatcgt
      661 ttagcaatcg ttttgacagc tgttttcttt ttctggctga agacgatcat tgcctactat
      721 gcagattttt cattaggtgt ggaaggtacg atccagtact ttatcttgtg gatcaaccca
      781 attgcgacta ccttattgtt ttttggcctt tctttgtata ttaaaaagcc aaagccagcg
      841 ttagctattc ttttgatcat tgatatttta aatacgcttt tgttgtactt gaatattatt
      901 ttttatcggg aattcacaga tttcattaca gtcaaatctg tattaggctt ttcgaaagtg
      961 tcgcaaggat tgtcaggaag ctccttttct ttgatgaagc cccatgatgt catctactgg
     1021 ctggatatcg ctgtgttcat tgggctactg gtttggttga aggtcaagaa aatacctatc
     1081 aaaagcaacc ctgtcggcaa accgatggct atcgctgtta cctgtctttc agggcttatt
     1141 ttttcaggaa atctagcgct gagcgaagcc aaccgtccac aattgctgca gcgaacattt
     1201 gaccgttcat atattgtcaa atatctagga atcgatgcgt atactattta tgacggaatc
     1261 aaaacaggaa tgactagcag tgttcgcgcg catgcaagca gtaatgggat tgatgaagtt
     1321 ttagattata caaagaaaca ttatgcagag ccaaatcccg aaacatttgg gatcgctaaa
     1381 gggaaaaatg tgatcgttct ccatttagaa agtttccagc agtttttgat taatatgaag
     1441 gtagacggac aagaagtcac accgttttta aatagtattt tccagaatca agcaacgatc
     1501 agctttgaca atttctttca tgaagtagga cagggaaaga caagtgatgc tgaaaatatg
     1561 ttggaaactg gcacatttgg tttacctcaa ggctcattat tcacagaact tgggtcagac
     1621 aatgtctttc aagccgcacc agcgatcctt ggacaaaagc aaggctatac aagcgctgtt
     1681 ttccatggga atgtagctag tttttggaat agagatcatg tatataaaaa cttaggttat
     1741 gacaatttct ttgatcgatc ttattttgat gaatccgacg aaacgttagg ttatgggata
     1801 ttagataaag atttattcag agaatctgcc caatatcttg agcatctaca acagccgttt
     1861 tacacgaaat ttctttccgt aaccaaccac acgccttatt atacggatga caagaatttc
     1921 gactttccat ctttgaatac tggcaatagc acagtagata attatgttcg aacggcacat
     1981 tatctggatc aatcattgga acaatttttt acgtatttga aaaaatctgg tatttaccaa
     2041 aattcaatct ttgtgattta cggggatcat tttgggatct cgaatacaga taataaagat
     2101 ttagcaagcg ctcttggtaa agatccagat acatgggatg aatttgacaa cgcacagatg
     2161 caacgagtgc cattgatgat ccatatgcct ggttatacta aagggacagt caatcatgaa
     2221 tacggtggag aaatcgatgt tttgccgaca ttactacatc tattgggaat cgatgataaa
     2281 gattatcttc attttggtac agacttatta tcttctcagc atgatcaagt agttgctttt
     2341 cgaaatggga actttgtgac acccaaatac accgtcctag gaggaaaagc ttacaataat
     2401 cagacaggag aaatcatcga tacgaaagca gcaggaatcg atgaggaaat ctccaaagat
     2461 caggaaaaag tcaagactgc gttaagtctt tcagataagc tgaatcagga aaatctcttg
     2521 cggttttatg tgccaaacgg atttgagacg attgatccga aaaaatatga ctataaaaat
     2581 gaagcggaac gtaatgaagc aatcgaaaag caaaaaggcg aacattcgac aagtgtcttt
     2641 tcaaaaaatg gtaataaaac aacaaccaac ttatatccag taacaagaac agacgttgaa
     2701 cagcaaaaaa ataatcaata aacaatcaaa ccaaccttca gtagttagac aacattctaa
     2761 tgcttgaagg ttggtatttt ttatgaaaaa aacatttttt tatttttttc gtgtattttt
     2821 cctttttaac gtatctattt attgaatgtg gaaaaattta aattaaaaag gagaactatt
     2881 atgtatgatg gtgaattgat caagaaactt cgagtcaaca agaagttgac acagtcccaa
     2941 ttagcggaag gaatctgttt taagacttct ctagtcggga ttgagacaaa ttcagtgaaa
     3001 aaaatgtcat ttgtaacatt gagatcattt ttagagcgaa tgaacatgac acttgcagaa
     3061 tatgaatgga tgcgaaacca gctggatgaa ccaataaagt taaagaaaaa aagacgaatg
     3121 ctagatcggg tacaagaaga cagctttgat ccttataaag agatcagcaa taaccgtagg
     3181 cattttaaga aaacagaaga tctgtattat ttggttttga atcttcacat ggtcgaaaaa
     3241 acagaagtat ctgagacaga atttgaattt ttacgttatg aatgccgaat cattgaagaa
     3301 tacttgatgc agataccaga ctatggtcaa tttgaactat ctattctagc tgattttcct
     3361 tggatattca gtgatcggtt cattgagaac aattatttga aaatcaaaaa aagaatgcga
     3421 cgattagcac agttgaacaa tcatgagcag tatctcttta cttttttaat gaacctgact
     3481 tcgttttata tagaaaatgg tcggttgaaa aaggcacgta gtgtgaatga agatatgaaa
     3541 cggagcttag cacataaaga aagaagtaca gtgatctatg aaacattgat ggcagattat
     3601 tatcaaagat tgatttctgc tgctttaggg gaagaaaaga cgaaagacta ccaagaattc
     3661 ttcacagtgc tcgaatatat gttaggaaag aatcaacgga ggtccttaga gaaaaaactg
     3721 ctggccattg ctgaacccag caactagacc agcagtttag aatttgtatc acttcgccgc
     3781 tttttcagac ggtttgttta tatcgaatgg cgggaaaaag cgggcgatct cttttaccgc
     3841 agcatcacgg gaatcagaag cgtgaatgat gttttcagtc cccggcagcg cgtagtctcc
     3901 tcggatcgta cctggaacag catcggcagc ttttgttgct ccaaccatct tacggacgat
     3961 atcgatcact tcctcgccga ccagcacaag ataaacgaca ggaccggaag tcatgtaatc
     4021 gatcagttca tcaaaaaaac tgcgctcagt caagtgctga taatgttctt ttgctaattc
     4081 aggagtcata gtttcaaact tcatttcagc aatggccagt ccctttgctt caaaacgttg
     4141 gatgatgctt ccgactaaat ggcgtctcac gccgtctggc ttgataataa caagtgttct
     4201 ttccataaca gcacctcgtt tcctactata tttctattat agcgggaggt ggaaaatgag
     4261 acaaataaaa ccgcgtgaga caggaatata acgtgtgtat tttaaaaaaa gtatgaaaaa
     4321 catggaaaat tgatttgaca tcctcttttt tagttgttat attaacgaag gaaaaccgaa
     4381 taagatttgt taggtgaggc tcctatacaa acataggcta ctgcccaaaa acgtcgagag
     4441 acgccaatgg gtagaacagg aattgtcgag ataaggcttt tcataaggta gctaaaagca
     4501 ggtatctgct ttttacgttg tacagtgtca aaactcaacg atgagatctg agaagctttt
     4561 tttaatatgc tttttcaaac ctttcgctga gtatttggcg agaggttttt ttgttgccgc
     4621 aaatccccat cggtgaaaaa taaaaaggag gtggtctaat tggaaaaata actattcgaa
     4681 aaatgattgg aagtcatgaa ttaaaattac ttagtaaagg agatgagaac aaatggatga
     4741 cagtcattcg aaaaacaagg atctagagga aaggccaggg ctattatcta tctgttctca
     4801 gcccctttca gttttttctc tatcgatccg atgaatagct aaagaccaaa agcagcgaga
     4861 acagctgtct tttggacaca aaggagagat tgagatggaa aaaaagaaag atacacaaaa
     4921 aatctatgca gcttttgctg gaaaaacgaa acaagaatta tttgaactat tccatgcatc
     4981 agcaaatgga ttgacagaag aacaaatcga acgttcaaga gaaaattacg gtgaaaatgc
     5041 gatttcatat ggcaaaaaaa caccattcat tgtagaaata ttgaaagcat acatcacgcc
     5101 atttacatta gtgttgatcg cattaggaat tatttcattc atcacggaat atgtattagc
     5161 agctcctgga gataaagatc ttttcggtgt agccattatc ttcactatgg ttatcgtcag
     5221 cgggacaatg acgttgattc agtccgtacg ttccaatcaa gcggctgaaa aactgaaatc
     5281 acttgtgaaa gtaacggcag ctgtaaaacg taatggaaag tacacagaga ttcctatgga
     5341 agaaatcgtc tgtggagatc ttgttcggct ttcagcaggg gatatgatac cagcggatat
     5401 gcgtttattg tccgcaaagg atttattcat ttcccaagca gcattgacag gggaaagcta
     5461 tccagtagaa aagcatgcga aagtatgtga agacgatcag caaagcgaaa caagttacga
     5521 aaatcttgtc tttatgggaa gtaatgtcat cagtggaagt gcggaaggaa tcattgtttc
     5581 tgttggaaat gaaacactat ttggtcatgt agcagaaacg ctatctgaaa aaccaattca
     5641 atcaagcttt gaattaggca tccataaaac atccatgcta ctgatcaaat ttatgtcatt
     5701 gatggctcca gcagttatcg tcatcaatgg gcttacaaaa ggggattggc tagaagcttt
     5761 tctttttgga ctatctgtag cagttggtct aacacctgaa atgcttccaa tgatcgttac
     5821 gacgaatttg gtcaaaggag caagtgtgat ggccaaaaaa ggaacagtga tcaaaaattt
     5881 gaatgccatc cagaactttg gtgcaatcga tgtgttatgt acggataaaa cggggacatt
     5941 gacacaagat aaaatcattc ttgaatatca tatggattgt gaaggaaatg aagatgatcg
     6001 tgttttgcgc catgcgtatc taaatagcta ctatcaaaca gggctgaaaa atttgttaga
     6061 tgtggctatc attgatgaag caacagaaac tttagataca gataagatca attatcaaaa
     6121 agtggatgaa attccttttg attttgaacg tcgtcgtatg agtgttgtgg ttgctgatcc
     6181 agctggtaaa acacaaatga tcacaaaagg cgcgattgaa gaaatgctga acatttctaa
     6241 atttattgat cttggcggta ctgttacacc attaacaaaa gaacgaaaag aagccgtact
     6301 tgcaaaagta cagtcactta atgaagatgg cttacgagtg attggtgtcg cacaaaagac
     6361 caatccaagc gtcgttggag aattttcagt caaagatgag tcagacatgg ttttgattgg
     6421 ttatcttgct ttccttgatc caccaaaaga aacaacgaaa caagcgttga aagctctgaa
     6481 agaacatggt gtagctgtaa aagttctgac tggggacaac gaattagtta cacgttcagt
     6541 gtgccgtcaa gtaggtctag agatcaatga attggttacc ggtgagaaaa tctcggaaat
     6601 gagtgatcat gaattagcac aagtagctga agaccatgaa gtattcgtaa aattgaatcc
     6661 tcagcaaaaa gcacgtttga cgacagcttt acgtaaaaat ggtcatactg tcggattttt
     6721 aggagatggt atcaatgacg ctcctgcaat gaaagtggct gatatcggta tttctgtaga
     6781 tacagctgtg gatattgcga aagaatctgc agacgtgatc cttttagaaa aagatttgac
     6841 gattttggaa agaggccttc tttctggacg agaaactttt gggaatatca tgaaatatat
     6901 caaggctaca gcaagttcta attttggcaa tatgttttcc gtgctgattg caagtacgtt
     6961 cctgccattt ttaccaatgc tgcctttaca acttttgttt ttgaatctga tctatgatgt
     7021 ttcctgcatt tccttgccat gggatcggat ggataaagaa tatttggacg agccaaaaaa
     7081 atggaatgca tctagtattg gggaattcat ggtttacttt ggtccaacaa gttcgatatt
     7141 tgatattact acttacttgt tgatgtattt tgttatttgt cctgctgtag tgggcggaag
     7201 tttccataca ttggatagtt cacagcgagt gattttcatc gcattattcc atgcaggatg
     7261 gtttgtcgaa tcactatgga cacaaacctt agtgctccat gcactgcgta caccgaaagt
     7321 accatttatc caaagcaacg caacatttgc catgtttgtt atcactacat taggaattat
     7381 tgtcggttct gtccttcctt ttacaagttt tggtgctgaa ttaggcttga tgccacttcc
     7441 tggcaattac tggttatggt tatttgctac agtcatcgct tatctggcgc ttgtgacagc
     7501 tgtgaagaaa atctatatcc gtcgattcca agaactctta taaacgagaa ggctggttct
     7561 ttgcttctgt tctttttgga ccagtcgtgc taaactatgt gtattgtttt ggaaaggatt
     7621 aatgtataga tgaaaggtgc aatatttgat ttagatggtt tgttagttga cacagaaaaa
     7681 acgtatcgtg atggctggct ttggggattt cagcaatttg atatccatat cccaaaagaa
     7741 atcgttgacg cttggggcgg aaaaaactgg aaacaaagtt ttgatatttt agtgaaagca
     7801 gcaggtagcc ctgaaaaagt acaggaagtc cgagcgaagc gtgaagaata tttttatcaa
     7861 cagctgacaa gcggagccat ccacttaaaa ccatatgcaa aagaaatatt ggcagactta
     7921 aaagaacgtc atatgaaact aggcattgct acgaccactg ttacgaaaag agcaacagat
     7981 atcttggatc actttaaatt agctgattac ttggacacgt ttacattcgg agacgaagtg
     8041 tcggagaaca agccttcacc gattccttat ctgacagctt tggaacgaac aggattagaa
     8101 gctagttcag cctttgcagt ggaagattct ttagtaggag caacagctgc ttcacgagca
     8161 ggattaggcg ttgttttgat acctgattcc agttttgtcc gaaattatac cgtcgaagaa
     8221 aaggaaaaat tgaaacttct tgcggaagga aaagatcttc gaactgttat tgaaatgt
//
LOCUS       DAGCYL010000125         8230 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.127, whole genome shotgun sequence.
ACCESSION   DAGCYL010000125 DAGCYL010000000
VERSION     DAGCYL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.127"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            330..1253
                     /gene="truB"
                     /locus_tag="MB216_002085"
     CDS             330..1253
                     /gene="truB"
                     /locus_tag="MB216_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBT4526883.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1257..2198
                     /gene="ribF"
                     /locus_tag="MB216_002086"
     CDS             1257..2198
                     /gene="ribF"
                     /locus_tag="MB216_002086"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBT4526884.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2593..3378
                     /locus_tag="MB216_002087"
     CDS             2593..3378
                     /locus_tag="MB216_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4526885.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3391..4242
                     /locus_tag="MB216_002088"
     CDS             3391..4242
                     /locus_tag="MB216_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBT4526886.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4366..4818)
                     /locus_tag="MB216_002089"
     CDS             complement(4366..4818)
                     /locus_tag="MB216_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4526887.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5283..5651)
                     /locus_tag="MB216_002090"
     CDS             complement(5283..5651)
                     /locus_tag="MB216_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBT4526888.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5801..6283
                     /locus_tag="MB216_002091"
     CDS             5801..6283
                     /locus_tag="MB216_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBT4526889.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6320..7330
                     /locus_tag="MB216_002092"
     CDS             6320..7330
                     /locus_tag="MB216_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBT4526890.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7445..8158)
                     /locus_tag="MB216_002093"
     CDS             complement(7445..8158)
                     /locus_tag="MB216_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526891.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2785 a         1337 c         1668 g         2440 t
ORIGIN      
        1 gattaagaaa ctgaaaaaat aaataaaaaa aaacggaaaa aagactggaa gaatggacag
       61 tctttttttt cgtttttggg aagtgaaaac caaactattt acctgtaagc cacccataaa
      121 tccaatcaaa tggtgcaaag aaaaaatctt ccattctatt ccctccttta aaaaaaacat
      181 aacatgattt ctatttacgt gagcatgaat tttgttacgt ttttattgcc gaaaaataaa
      241 aaccgttcat tatgatgtca aattcgtcag taattgatgc aatgtgttat aataattcag
      301 ttaaatcaaa aatgaaatgg agaaaagaca tggacgggtt attaacttta tggaaagaac
      361 gcggaatgac aagtcatgat tgcgtattca aattaagaaa aatcttacat acgaaaaaaa
      421 tcggacatgg gggtacgctt gacccagacg tagacggtgt gttgcccatt tgtattggaa
      481 aagccacaaa agttatcgaa tatttgacag attctgggaa aatctatgta ggcgagataa
      541 ctctaggttt cagtacaacg acggaagatc gatccggaga acgggtagaa acaaaaccag
      601 tagatatgtc tttatctgaa gaaaaaatag atgaagtgat ggcttcattt attggtgaga
      661 tcacccagat accaccaatg tactcagcgg taaaagtcaa cggtaagcgg ttatatgaat
      721 atgcccgcaa taatgaaaca gtggagcgtc ctgttagaaa ggctcagatt taccgtttta
      781 tccggacaag tgaattgaca agagacgaag tgacaggaac catttcttgg cgcttcgaag
      841 tagaatgcgg gaaaggaacc tatgtccgca cattagctgt agatacaggc aagaaacttg
      901 gttaccctgc gcacatgtct gatctgaccc gaacagcaag tgccggtatg tcccagaaag
      961 atgctctgac attagaagaa gtagctcaag ctgtgaaaaa tgggacaatc gatgattgtt
     1021 tgctgccaat agaaaaagga gtgaaacgat tcaaacgagt agatattact gatgaagtat
     1081 ggcagaaagt aaaaaacggt atgagactta attatactgt atttgcgctc ccagaaatgc
     1141 cgtcagaaga aattgctttg ttttataaag gcaaagttgt cagtatctat cagccaaacc
     1201 cgaaagaaaa aaacaaatta aaacctagta aagtattgag aaatgaggaa taacgaatga
     1261 atatcatcaa gatcagacat ccttaccaat cagaacaaat tccagaagac gatgtcgttt
     1321 tagtactagg attttttgac ggcgtgcata ttggacacca gaaagtcata aagacaggta
     1381 tggaaattgc acaaaaagaa ggattgaaat tagcattgat gactttcaat cagcatccat
     1441 cgattgtctt taaaaagatc gatcctagca gcgtgaaata tttgactagc cttaagcaaa
     1501 aagaagaaaa aatggccgct ttgggtatag attatttata tgaagttgat tttacttctt
     1561 cttttgctca tttagcaccg caggaatttg ttgatcaata catcgtaggg ttacatgcaa
     1621 aatatgctgt ttccggtttt gattatacgt atggaccaaa agacattgca gatgtcgctc
     1681 attttcctga atatgccaaa ggaagattca aaatcgtcac agtttctaaa gaagaagaac
     1741 aaggtgaaaa aatcagctct acccgtattc gaggattact agacagcgga caagtaaaag
     1801 aagcagcaaa actattaggt tgcacatatg aggtagaagg ggtagttgtc catggcgatg
     1861 cacgaggcag actactagga ttccctacag caaatgtcaa agtaaagagc acagttcatt
     1921 tgcctaaaga aggtgtttat gtatcggaaa taaaaattgg ggaaatttgg tatccagcca
     1981 tgggttcaat tggacataat gatacattcg gtgaaggacg ggagctgact gtagaattgt
     2041 atattttgga ctttaatcaa gatatctatg gtgaacatgt cgcagtacgc tggaatcaat
     2101 ttttaagaga tcaagtgaaa ttttctagtg tagaggaatt gattgaacaa ctcaaagagg
     2161 atgaacaagc aacagctgaa tatttttctc agcaataaaa cattatgcat aaaatctctt
     2221 gtttatgtat aactagcaat ttcaataaaa tgggtgataa ataagcccag gaaaggggct
     2281 gtgacaaaag tcttgtcaca gccttttttt ccaaataaat ggtttttcag aagtagcttc
     2341 ttcggaaata agccgaaatt tcacaaaaat ttgagaagta atttttgtaa attacttctt
     2401 atttcttgaa gctggccact tctgtcacaa cctctttctt tatagaaata aaaggacact
     2461 agcaattcat gaaaacgatt gctaaagcgt atcatttgct ttagactata tatcttggtg
     2521 tacactcact ttaagacgga aataaaagtt atccgtaata acaggtttag gtatgaaaaa
     2581 ggagtgggta ctatgtataa ggaattagaa aacaaagtcg cagtagtaac tggtggctca
     2641 aaggggattg gaacagcgat tgctcaacga ttcgggaaag aaaaaatgaa tgtagttgtc
     2701 aactataact cggataaaga aggagcagaa aaagcagtca aagcaatcga agaggcaggc
     2761 ggaaaagcag tctctgtaca agcagatgtc agttctgaag aaggaataca gcgtttactt
     2821 gatacggctt tagaaaattt tgggacactg gacgtttgga ttaataatgc tgggatggaa
     2881 aatcagcatc caacccatga acttagcctt gaagattggg aaagagtatt aaaagtcaat
     2941 ttgactggcg tttttatcgg aacgaaagcg gctttaaatt actttacaga gaatgataaa
     3001 aaaggaaata tcatcaatat gtcttctgtt catgaacaaa tcccatggcc aacgtttgct
     3061 cattacgctg cttcaaaagg cggagtaaaa ctattcaccg aaacggtagc aatggagtac
     3121 gcaccaaagg gaatcagaat caatagtata ggacctggtg ctatccgtac acctattaat
     3181 gcggcaaaat ttgatgatcc tgaacaaaaa gaactcttgg aaagtatgat cccactagga
     3241 cgcgtaggtg aaccaaaaga aatagcggcg acagctgcat ggttggcttc agacgaatcc
     3301 agttatgtta caggtattac actatttgca gatggtggga tgactttata tccttctttc
     3361 caaggtggaa aaggataagg gtgaataaaa atgggtttat tgattgcatt gatcccggca
     3421 gttgggtggg ggatccagcc actgattttg aaaaagattg gtggcagacc aacaaatgag
     3481 atattaggaa ctggtattgg agccgtgctc gttggcttac ttgttcaatt attcgcttct
     3541 ccagctggta tttcagtgac tacatttttg attagtttac tttctggtgc attctgggtc
     3601 atcggacaaa caggacagta tacatctttc aacttgattg gtgtttctaa aacgatgcca
     3661 atttcgactg cattacagtt agtcggaacc tcattgattg gtgtcttcgc ttttggagaa
     3721 tggtctggaa gtatgggaaa aatcattggt gcagcagcaa tcattcttct tgtatttggt
     3781 tctgctttaa cggcaatcag tgatgatcct ggaaaaaaag gcggtatcac aaaaggaatc
     3841 agtctattag catcaacaag tattggttat tgggtttaca gtgcattgcc caaactagtt
     3901 gatgcaagcg gactttccat tttctttccg caaatgatcg gtgtcttttt aggggcagtg
     3961 atttatgtga ttttccgaaa accaaccgca ttttctgaag acaagagttg gaaagcaaca
     4021 atagttggag ccatcttcag tgtgtcagca ttggcttata ttttttcagc aaatgaaaat
     4081 ggggttgcta ctgcatatat cattacacag ctaaacgtag ttatctctac attaggcggt
     4141 atgtacatcc tgcatgaaaa taagacacca aaagaaaaaa aattaacgat tatcggatta
     4201 ttgctgatcg tagtaggaag catggtcacg gtctttattt gaggataaag atggtaaaat
     4261 ctcatttatt aaaaaaatat ttgtttgaac caaacagtaa gtacttataa aaaaatagcg
     4321 ctagaatttc caacatctct ggaatttcta gcgcttttta attgtttaag atacaagtga
     4381 aatatctgtc tgcatcaata atttttcatt gatccattga ctaaaattta cgacatgatt
     4441 gaagttacca ccgattagta tctccgcatg attgtcttta taaagatcgt attgtaaaag
     4501 acggtctttt ttatttgcgt aaacatcgtt atggtactgg actaattgtt ctcggatttg
     4561 attgattgct gtaaaatcac gatactgaag gttgaaacgc atcgtgattg gtgcatttag
     4621 catgaatcgc tgcatttctt ttgctgcttc ctctgcttga tctccgtata ggaactcaaa
     4681 aagattacgt tcagaaaatt tccattgccc gccaaccttt gttcctttta gttttccttc
     4741 atgaagataa tttctgatag aacgtgtagt aacacctaat tgttcggcta attcatttac
     4801 tgagtaaaaa tctttcaatg ttcaccctcc tatgtgaatg gttctactga cttagttgat
     4861 atttgcttaa tcacgaaatg tagaaagaac gattttaatt aaggtcattg taaaaatcaa
     4921 gtcagaaaat tctaactttt ctttctctaa agaaattcgt tctctcctaa agtgatatta
     4981 tatttaaata tacctgaaaa taatgggcag tattatgaaa gatcactgat attttctcgt
     5041 attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt ttactaagtg
     5101 gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact ccttataaaa
     5161 atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga taggattgaa
     5221 caaaacctgt ataagctgat gaatagtttc ctcgccagta aaaggtttat tcgtataaag
     5281 ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga aatcaattgt
     5341 tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt atccgtatat
     5401 acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt ttccggtgcg
     5461 atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg gtcagtaaac
     5521 ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt tatcttagca
     5581 aaaaggtctt tgatcttttg ttgatgttct ttttctttga tctgattgat gatcggctga
     5641 aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc agaaaaacaa
     5701 tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat atttatatga
     5761 taagatgtct caaataataa aatagaaaag agacataaaa atgattgact tggcaaagaa
     5821 attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt atacaaatga
     5881 agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca aaagcttatt
     5941 cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga aacaaacgga
     6001 tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat cagaacgcat
     6061 ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg aaaaacagat
     6121 tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg caccaggtag
     6181 accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc agctacttga
     6241 gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta aaatagtgca
     6301 tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg caacgattgg
     6361 ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg aatcagcttt
     6421 aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt ggacgcaaga
     6481 taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa tgatgcaaga
     6541 tgaaaatatc caaatgatct ttccgccttg gggaggagaa ttattgattg agattctcga
     6601 ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag atattagtac
     6661 gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa ccaaccttat
     6721 tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg tactgaaaac
     6781 aagagaagga gaatcgattc tacagcaacc ttctgaaaag tatcaaaaag agtggcccca
     6841 tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc aatcagtcag
     6901 cggagatgca gtaaggatga agggacgatt attaggtggc tgtattgata cgatcagtca
     6961 tttaatcggt accccttatg gagatgtcca atcttttaaa agaaagtata atgatggaaa
     7021 gccgatcttg tggtttctag aaaattgtga gctatcgtca gttgatttaa gaagatcact
     7081 tgttcagatg aaactggcag gatggtttga tggcagctcg gggatcatgt tcggtagaag
     7141 tgcagcagag gatttgttag attataaaat gaaggatgta tatgaagatc tgtcaaagga
     7201 attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac aactgacatt
     7261 gatcaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg ttttgcaaac
     7321 gtttatttaa aaaatttttt cctgccttgg agtttatcaa agaccaacaa atctaaatag
     7381 aaaagaaaat gaaagaagct gacagtattt tgccagcttc tttttgtatg atctattaga
     7441 aattttaatt atccataact atcacattga tcagctgttt cacatcattt ggattacctg
     7501 taggaatagc tactacagat gcattatcaa taatttcttg agttgtttct ctaatgaacg
     7561 ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga taactagtga
     7621 ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat ttcccagtga
     7681 gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg ataggtgttc
     7741 ctgcaggata atgaataaca tgtagttcga aatcacccga tgtatctcta aaagtattac
     7801 ctctaattgc tagccaaaca cgtgcgtact caatttgtag atcagaatat cctgctaacg
     7861 gatctattga ctgagaagac tgtgaggaag ttgtgtctat tgttgaagaa gaaatatttg
     7921 tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta ctgcttgttg
     7981 aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca gctgaactgg
     8041 tttgtttgat tgaacttttg tctgtggtcg cagatggatc actattttct ttactaggag
     8101 acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac tttttcatat
     8161 aaaatacctc attatttttt taataccagt atagcataat cctgaatttt tgaatccgta
     8221 aaaccttgtc
//
LOCUS       DAGCYL010000126         8104 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.128, whole genome shotgun sequence.
ACCESSION   DAGCYL010000126 DAGCYL010000000
VERSION     DAGCYL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.128"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            3..1457
                     /locus_tag="MB216_002094"
     CDS             3..1457
                     /locus_tag="MB216_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HBT4526892.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1533..2693
                     /locus_tag="MB216_002095"
     CDS             1533..2693
                     /locus_tag="MB216_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBT4526893.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2806..3813
                     /locus_tag="MB216_002096"
     CDS             2806..3813
                     /locus_tag="MB216_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4526894.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3837..4862
                     /gene="iolG"
                     /locus_tag="MB216_002097"
     CDS             3837..4862
                     /gene="iolG"
                     /locus_tag="MB216_002097"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HBT4526895.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            4879..5769
                     /gene="iolE"
                     /locus_tag="MB216_002098"
     CDS             4879..5769
                     /gene="iolE"
                     /locus_tag="MB216_002098"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HBT4526896.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5792..7387
                     /locus_tag="MB216_002099"
     CDS             5792..7387
                     /locus_tag="MB216_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBT4526897.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7403..7717
                     /locus_tag="MB216_002100"
     CDS             7403..7717
                     /locus_tag="MB216_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBT4526898.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
BASE COUNT         2414 a         1534 c         1939 g         2217 t
ORIGIN      
        1 aaatgacaag atttttaaga aattatattg atggtcgttg ggtcgctagt cgctcaacag
       61 agtttgaaga agtaatgaat ccggctacag aagaagcgct ggcgaatgta ccgttgtcta
      121 gcaaagcaga gacagccgaa gcagttaaag ctgccgatgc tgcgtttcaa acttggaaaa
      181 aagttcctgt tcctgttcgc ggacgttatc tgttcaagct gcatcagctt ttaacagatc
      241 ataaaagtga attggcgcgt ttgatcacgc tggaaaatgg gaaatcattg tccgagtcac
      301 tgggagaagt tcaacgagga atcgaaaatg tggagcacgc agcgggaatc accaatttaa
      361 tgatgggaga ttctttatca acagcggcga ctgatattga gatcacgaat tatcgttacc
      421 caatcggagt aatcggaggg atcacaccgt ttaattttcc aatgatggtg cccttctgga
      481 tcttcccgat ggctctggcg gcaggaaata cggtggtgct gaagccttca gaaaaaacac
      541 cattgttgat ggagaaaatt gttgagctgg ttgaaaaagc aggctttcct agcggtgttt
      601 ttaatgttgt ctatggtgcc cacgatgttg tcaacacatt gctgcaagat ccactggtaa
      661 aaggcatcag ctttgtagga tcaaaaaatg tcggtgaata cgtttaccgt gaaggaacaa
      721 aaaatctaaa acgggtacaa gcgttgactg gagcgaaaaa tcatgttgtt gtgctgaaag
      781 atgccaatat tgatcgggca gtcaaggacc ttattggcgg agcttttggt tctgctggtg
      841 aacgctgcat ggcagcctcc gtcattgttt tggaagagga aatcgcggat cagtttgttg
      901 aaaaatttaa ggctgcggca aaagcgatca agatcggcaa tggcttggac gaaggggtct
      961 ttttaggacc agtgattcga aaagatgcac agcagcggac ctttgactat atccaaaaag
     1021 gggtcgatga aggagcgaaa ctggtactag atggtcgcga aaacattcca gaaaaaggct
     1081 attttgttgg accaacgatt tttgataatg tcacgatgga tatgaagatc tggcaggatg
     1141 agatttttgc cccggtcgca tcaatcgtgc gggtaaaaga tttgatggca gccgttgagc
     1201 tgacgaatga atctgaattt gcgaatggtt cgtgtctgta taccaacaac gcatcagcga
     1261 ttcgctactt cagagaaaat gttgatgctg ggatgctggg ggtcaatctt ggggtgcctg
     1321 caccgatggc ttggtttgcc ttttcaggct ggaagcactc tttctttggt gatttgcatg
     1381 ccaatggcaa agacagtgtt gagttttata cccatcggaa agttgttaca gcacgttatg
     1441 cgcaaagtga gatttaaaga tttgcttcga agctctgtta aagagcttcg aggattctgt
     1501 tataggctat aagccagaga gaggtttttt ctgtgaatat tcaactgaaa atgcctgcca
     1561 atttaagaat tggcggcgga acaatccaat ttcttatgga agaatcagaa caactaggca
     1621 acaagccgtt aatcattagt gatacggtta tggaacagac cgggcatctt caaaagatca
     1681 aggagctgtt gggaaaaaca gatgatcaat tagcggagta tacagacatt caagccgaac
     1741 cgacgcaaga aaatgtttat gatgcgctga agctttttca gaagcatcaa tgtgatcata
     1801 tcattgcttt tggtggcggc agctgtctgg atacagccaa ggtggtagcc gttttggctg
     1861 ctaatcaatt aagtctcgaa gcgattcatg caagaaattt tgatcagctg caagcgggca
     1921 ttccgctgat tgcagtgtca acaactgctg gaactggttc ggaagtaacc gatgtggcag
     1981 ttattaccaa tacaaaaaca gcagtcaaaa tgatgatgaa ggatctcgtt ttcatgccac
     2041 agatctcaat cgttgactct gattttacga aaactgtacc accaaaagta accgcggcaa
     2101 cgggagtcga tgcgctatgt cacgctttgg aatcttatgt ttcagtcaaa gccaatgaag
     2161 taacgcaact attttcactg gaagcgatga agaaaatttt gaacacgctt tctcaggcat
     2221 atgaaaatag cgacgacact gaagccagag aacaaatgag cttagcttca acggaggcgg
     2281 ggattgcctt ttcgaatgcg tcagtcacat tgattcatgg aatgagtcga ccggtggggg
     2341 cactttttca cattcctcat gggatatcaa atgcgatgtt attgccggtc ttttttgaat
     2401 tctcaaagga tgctattcag actgagttag cgacgatcgc cagatttatt tatccggcag
     2461 ccgaagattt atcgctggaa gaggctgcgg attatctgat taaaaaaatc caagagctct
     2521 gtgagcagct gtcgatccca acgattcgag attatggtat tgatcagcaa gcgtttatgg
     2581 cagccttaga caaaatggcc gcggatgcgt tgaccagcgg aagtccgcaa aataataaaa
     2641 aggtgcctag tcatagtcaa attaaggaat tatacgtaca agcttatggg tagtaaaagg
     2701 cggagcaaag agagggctct accttttaag aataaaagct acacaggaat aattttgtaa
     2761 gcgcatacta tgtgtgctta aacaacagaa cagggggata agtaattgag agtaaaagta
     2821 ggcgttatcg gaacaggtgc aatcggcatc gagcatatcg acaggatcaa tcagaaaaca
     2881 caaggggctt atgtgacagc agtctcagat atcaatgtaa aagctgctaa agaggttgcc
     2941 aaacgtgtcg atgctaaatt ctttgaaacc ggagaagcat tgatcgcttc tgaagatgtg
     3001 gatgtcgttg ttgtgacttc ttgggacccg acccatgagc agtatgtgtt agaagcaatc
     3061 aagcatggga aatatgtttt ctgcgaaaag ccattagcga tggatgcagc aggctgccgc
     3121 cggatcgttg atgcggaagt tgcttatggg aagaaattgg ttcaggttgg cttcatgcgc
     3181 cgctatgacc gcggctacat tgaactgaaa gaagcaatcg aatcgaagca atacggagaa
     3241 gcattaatga tgcattgtgc ccatagaaat cctagcgcag atgaaaacta cacaacaccg
     3301 atggcgatca gcaacaccgc gattcatgag attgatgtgc ttcgctggtt gctaaatgaa
     3361 gattatgaat ctgttcaaat gatcttgccg aaaaaaacca gcaactccca tgccgatttg
     3421 catgatccgc agctgctgat ctttcaaaca aaatccggtg taacgattga tttggaaatc
     3481 tttgtgaatt gccgctttgg ctacgatatt aaatgtgatg tggttttcga aacggcagaa
     3541 gtaggaatga cggacccagc ctatacacga attaaatcgg aagaaaaaaa ttacacacct
     3601 gtttcaccag attggcagac ccgctttttg gatgcctatg attatgaatt caagctgtgg
     3661 atcgattcga tcaaaaatga tgcggttgtc ggtcctaccg cttgggatgg gtatgtagca
     3721 gcgatcacca tgatggcttg tcatgagtct cgtgaaacaa aaacaaaagt aatgattcaa
     3781 atggatgaac gaccagcact atacaatgag taattctcag aaaacggagg tatttcatga
     3841 acaacattag agtaggcatc gtcggtttag gaagattagg aaaagtacat gcacaaaatt
     3901 tagcaacagg tgtcagttgt tgtgagctta cggctgcgtg cagtttagtt gaaacagaat
     3961 tagcattcgc acagaagaaa ttaggcgtca agcatacttt ttccagctat gaagaaatgg
     4021 tcaaaagttc cgtgatcgat gcagtcttta ttgtttcacc atcaggcttt cactgtgacc
     4081 aagtccgctt ggcgatggaa aatggcaagc atgtctttac tgaaaaacca ttaggccttg
     4141 aagttgaaga tattaaaaag acccaaaaag tcatcgaaga gcatcctgaa caggtcttca
     4201 tgttaggctt catgcgccgc tatgatgagt cgtatcagta tgcgaagaaa atggtagaag
     4261 atggcgaaat cggcgaaatc acactgattc gttgttatgg gatcgatccg agcgaaggct
     4321 tggaaagctt tgtcaaattt gccatgaata acgacagcgg cggcttgttc ttagatatgg
     4381 cgatccatga tattgatttg gttcgctggt ttaccggcca agaagtcgat caagtgtggg
     4441 cgattgggaa aaatgcggcg tatccagaat tggatcaggc tggtgagctt gaaactggag
     4501 ccgcaatgat gagcctgagc gataaaacaa tagctttact agttgccggc agaaacgcag
     4561 cccatggcta tcatgtcgaa acagagatca tgggaaccaa aggaatgctg cgggtagcgc
     4621 aagcgcctga gaaaaatctt gtgacgatca tgaacgatca agggatcatc cgtccatgtt
     4681 cacaaaactt cccagaacga ttcagagaag ccttcattcg tgaagcccaa gaatttgtct
     4741 catgtattca agaaaaacgt caaccagcgg ttaatgcaga agatggattg caatcaacta
     4801 agatcgcgtt ggcttgtcaa gaatcattcg atacgaagag tttagtacaa ctaaaaaaat
     4861 aagcaggagt gtgagaatat gaccaaacaa attcaattag ccatcgcacc aatcgcgtgg
     4921 accaatgatg atatgcccga gcttggtagt gaaaatacct ttgaacaatg tatcagtgaa
     4981 atggcccttg ctgggttcac cggaacagag attggcaaca aatatccaaa agatccagag
     5041 gtgttggaac aatatttgaa tattcgcggc ttaaaggttg ccagtgcatg gttcagtgcg
     5101 tttctgacaa ctaaaccttt tgaagaaact gccgaagcct tcaaagcaca tcgggatttc
     5161 ttacatgcga tgggcgctaa agtcatcgta gtagctgagc agggtcatag tatccaaggg
     5221 attatgacag cgccagtctt tgatgataaa ccagttttca ctgatgaaga gtggaaaaaa
     5281 ctggcggaag gattagaaaa attaggggat ttggcccacg aaaaggatat ggagatcgtt
     5341 taccatcatc acatgggtac cggcgttcaa acgacggaag aaatcgatcg tttaatggaa
     5401 atgactgatc cagacaaagt taaattattg ttcgatacgg gacatttggt cttttctggt
     5461 gaaaatccaa tcgcggttta caaccgtcat caagatcgta tcaaacacat ccatttcaaa
     5521 gatattcgtc aagaaacggc tgaagaagta gaaaaggaaa aagacagctt cttaaacggc
     5581 gtgaagaaag gcgtcttcac ggttcctggt gatgggatga ttgatttcac tccaatctgg
     5641 acagcaatcc atgccagcga ctatgaaggc tggatcgtag ttgaagcaga acaggacccg
     5701 gctaaagcca atccatttga atatgcagtc aaagccagaa gctacattcg agaggtcacc
     5761 ggtctttaat ctattgaaaa ggagctagac gatgaatctt ttttctataa tcagtttcct
     5821 agtgattgtc atctgtgtat ggttatttgc ctattctaga tccagaagtg tcgatacttc
     5881 tggatcagaa gggttcttca tgggagggcg gagcttaact gctttgccaa tcgccggcac
     5941 gatcatcatg acgaacctgt ccaccgaaca aatagtcgga caaaatggtc aaagttatca
     6001 tgcagggatg gaagtaatgg cctgggaagt aactgctacg attgcgatcg tagctttggc
     6061 tgtgatattc ttgccaaaat attttaaata cggcatcaat acaatctcag atttcatcga
     6121 gattcgttat gatacggtta ccaaacgaat tatttcgatc ttatttattg tgacctatat
     6181 gacttctttc ttacccgtgg ttctttattc tggatcgctg gtgttcaata agatcttcca
     6241 tattgatgag ctgctgggcg tccagccgat cgtcgcgatc ataattgtag cgatggcaat
     6301 tggtattgtc ggcatcctct atctgctgat cggcggcttg tcgttaagtg ccaatagtga
     6361 ctccatttat ggagtcggtc tgctggcttg tggtctgctg gtcccaattt taggcttaat
     6421 gaagcttggt gacggcagct ttgttggcgg gatcgaacac gttgtggtaa acacgccatg
     6481 gctgcttaac tctgtgggat cgatcgactc ggctgttgtt ccatggccga cactatttac
     6541 agggatgctg tttaacaatc tgtatttctg gtgtaccaat caaatgatcg tccaaaaagc
     6601 attgtccggc aagaatttag ctgaggcaca aaagggcgct ttcctagtcg gcgtcttcaa
     6661 aatcttaggt gcgttattcc ttgtctttcc agggatcgtc gctcgcaata tttttggcga
     6721 tagtttaatg tcaaatccag ataacgcgta tcctttttta gtcagtgaag tattgcctaa
     6781 aatgatcttt ggtattttcg ccgctgttat ctttggcgcg atcttatcat catttgcggg
     6841 agctttgaac gcgacatcga ccttgttttc actggatttt tataaaccgc ttatcaataa
     6901 gaaagcaaat gataagcaaa tcgctcgtgc tggtaaaaca gtaacggtcg ttgtggggat
     6961 tatctccatt atcgtagcgc cattcatttc ttttgcaccg gctggattgt atcagttcgt
     7021 tcaagaattc aatggactat acaacatgcc gctgctcgtg atcattctct ttgcctttta
     7081 ttcaaaaaaa gcaactgctt ttggggcaaa aacgacgatt gccgtgcata tcgtcttgta
     7141 tgcactatca aaaatgcttt tccaaaatat ccatttcctt tatgttttga cggcattatt
     7201 cgttctggac attatcattt tgatggtggt ttccaaattg aaacctgatg gagaatttgt
     7261 gttgaactca ttcgatacaa aggtcgatat cgagccttgg aaatacacca aggtgactgc
     7321 tatagcattg gtagtcattg ttattttaac ttatgtggca ttttcaccaa ttggctttgc
     7381 aggctaaaaa caggagggta ttatgcataa ggatctattg attatttgcg agaccggaat
     7441 cagtgcgtcg ctgctcgtat caaaaatgct tgaaacgatc agagccaaaa atctatcgta
     7501 tgagatagat tatgcaacga tccgaaacgt tgaagaaaaa ctgaattata aagactatga
     7561 cgttttactt ttaacgccgc aggtcgcacg ttctgaggaa aaagtaaaag aaatcatcaa
     7621 aaaggaaaaa tgtaccgctc agatcgttta tatcaaacaa gaagattttc gttatatgaa
     7681 tattgaaaac atcattgcaa gcatcgaaga agattaacga gaatctttga ttattgagtg
     7741 tcgaggaggt ggtatatgaa aagatggcat gttcgccccg atcaccccaa tatgaagggc
     7801 ggacttatta gaaataccca atcttgtatt tattcaatag ggtaagcgga cctcaatagc
     7861 cagctcatcc ccaaaaagtc gaatggagac acttaaatga gtgtacctat tcgacttttt
     7921 gagactctaa gtcaaatcgt gttgttctca ttaaatcact gaaaggaaat gcttcggcgt
     7981 ttcctttttt ctttttgttt ttcttcgtgt tttgaaaatt acttactatt tactatacga
     8041 gtagtttcaa ataattttga catcctaatc ttcaacaaca gaaaaaaatc taaattataa
     8101 aatt
//
LOCUS       DAGCYL010000127         8000 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.129, whole genome shotgun sequence.
ACCESSION   DAGCYL010000127 DAGCYL010000000
VERSION     DAGCYL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.129"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(28..876)
                     /locus_tag="MB216_002101"
     CDS             complement(28..876)
                     /locus_tag="MB216_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4526899.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1100..3040)
                     /gene="thrS"
                     /locus_tag="MB216_002102"
     CDS             complement(1100..3040)
                     /gene="thrS"
                     /locus_tag="MB216_002102"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBT4526900.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3377..3556)
                     /locus_tag="MB216_002103"
     CDS             complement(3377..3556)
                     /locus_tag="MB216_002103"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBT4526901.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3603..5426)
                     /gene="cadA"
                     /locus_tag="MB216_002104"
     CDS             complement(3603..5426)
                     /gene="cadA"
                     /locus_tag="MB216_002104"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBT4526902.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5704..7983
                     /locus_tag="MB216_002105"
     CDS             5704..7983
                     /locus_tag="MB216_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4526903.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2346 a         1704 c         1448 g         2502 t
ORIGIN      
        1 aaacttatat aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc
       61 ttctcgtgtt tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa
      121 aatgatatcg atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt
      181 gatttctttt ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt
      241 tccaaattgc gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc
      301 gactacatct gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt
      361 gctgacaagt tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac
      421 acgagaccat ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa
      481 aaaaacagtc tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag
      541 aatatctact gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga
      601 gtaaaaggac tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc
      661 tgttgaaatc gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg
      721 tacaactgtc ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg
      781 gaccttctca atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc
      841 ttccatcata ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat
      901 agtctatgta agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca
      961 aaaaaacaat ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca
     1021 tctgtgtctt tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt
     1081 tttacaggaa atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag
     1141 catcaataaa gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt
     1201 tcactgttgc atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg
     1261 tttgagaggc acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca
     1321 accccttcat ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag
     1381 aaacagggat gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata
     1441 cttctgttaa ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta
     1501 ctggacgatg cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca
     1561 ataagaagtc taattgtata gttgataatg tttcttctgt accaagagct gttttgactt
     1621 gaacatctag ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt
     1681 ctaattcatc catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat
     1741 caaaatattt atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa
     1801 tattgaagtc agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga
     1861 tttgatcagg acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta
     1921 aacctgacaa agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa
     1981 ttggtaattc acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt
     2041 tcattgggcg aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt
     2101 cttgataatg atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg
     2161 tatatacatg ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc
     2221 gtcggattgt tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa
     2281 ccatgaacaa gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac
     2341 ggagacggat atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt
     2401 gcatcatttg attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa
     2461 atacttgtat acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc
     2521 cttgttgata agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt
     2581 acggatcact tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg
     2641 gcagattttc tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag
     2701 ttacttggtt ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa
     2761 cgccaaagtg gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg
     2821 aatgacggac taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat
     2881 cttcctcgat tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt
     2941 tttttgctaa gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa
     3001 attctttgac tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat
     3061 gttttgattg atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag
     3121 agatactagg acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca
     3181 agcgttgtaa cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt
     3241 ggttttctcc tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt
     3301 cagttggctt tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc
     3361 aagtgggaaa ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca
     3421 tctcattgat gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt
     3481 ccgtgatatc tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga
     3541 catgtacgaa tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc
     3601 tatcatacta tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct
     3661 ctcaaggcac tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat
     3721 gcggggatta gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc
     3781 agtacagact gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca
     3841 cgcaagagat catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca
     3901 tgagccccca ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg
     3961 tctcctacca tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta
     4021 tcttgaggca agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga
     4081 gcaatggcag atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc
     4141 tccttgattg tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca
     4201 aaagatcgat caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa
     4261 acaaaagagg cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga
     4321 ccggtcactt ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact
     4381 aacgaacgag ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc
     4441 tcttcttttt ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta
     4501 agcgttcctg ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt
     4561 ccagttttta caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg
     4621 ggcgcagcta gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa
     4681 gggtcttttg agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg
     4741 actgcataac gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact
     4801 agtttaataa tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc
     4861 gatccgtctc catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg
     4921 cgggactcac ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca
     4981 tctgcgggta caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg
     5041 attactatat cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct
     5101 aacaaagact gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg
     5161 cccgtaagca tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca
     5221 aggattgcgg taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc
     5281 atttccaaaa acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca
     5341 agggccggct ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta
     5401 agtaaaaatt ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg
     5461 ttagtaataa atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt
     5521 taaaacaata ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag
     5581 ttctacaaac aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc
     5641 ttttttcgaa aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat
     5701 gcgatgaatg agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag
     5761 atcgaacaaa atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg
     5821 acaaaagaca cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct
     5881 gctgttgaat atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca
     5941 cagcaaaaac gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc
     6001 gactttaaag aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat
     6061 ctggcagaag aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa
     6121 ggtgagattg gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag
     6181 ttgaaacgac aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa
     6241 gtaatcaatg atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa
     6301 aacatcgtct ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa
     6361 gtaatgctga ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt
     6421 gcttttctgt tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca
     6481 cccaaccatt tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc
     6541 gtgcctacac agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta
     6601 gatgaacagc aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg
     6661 aaaagcggat tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac
     6721 ggtcccttgt tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt
     6781 cgccaatggt ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta
     6841 caaacgaagc tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg
     6901 gtcaaagatc tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa
     6961 tatacagaac aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa
     7021 agattccgtc gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa
     7081 tatctccact ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag
     7141 accaggtggc aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt
     7201 caagaggatg ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca
     7261 cacaaagctc gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca
     7321 ttgctgcaag ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat
     7381 gtctttggaa atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc
     7441 actcgtttcc aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat
     7501 cattttattg tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg
     7561 ctcatccaag gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg
     7621 actcagggaa aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc
     7681 gaagctcttt atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat
     7741 gagagtgact ttatgaaacg ttccattatt ttacttccag cctttttagc aaaaggtctg
     7801 gagtttgacc gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac
     7861 aaattgatcc tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc
     7921 aataatgaaa gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg
     7981 taagtaaaaa gacaactgct
//
LOCUS       DAGCYL010000128         7957 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.130, whole genome shotgun sequence.
ACCESSION   DAGCYL010000128 DAGCYL010000000
VERSION     DAGCYL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.130"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="MB216_002106"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="MB216_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBT4526904.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="MB216_002107"
     CDS             complement(2203..4389)
                     /locus_tag="MB216_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBT4526905.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="MB216_002108"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="MB216_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBT4526906.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="MB216_002109"
     CDS             complement(4611..5051)
                     /locus_tag="MB216_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBT4526907.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="MB216_002110"
     CDS             complement(5125..5664)
                     /locus_tag="MB216_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HBT4526908.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6279..7745)
                     /locus_tag="MB216_002111"
     CDS             complement(6279..7745)
                     /locus_tag="MB216_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4526909.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2164 a         1741 c         1379 g         2673 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aacttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     6241 cacttctgtc acaacctctt gtaacaagta tttctatttt aaggctgaac cactgtttct
     6301 ggttcagtga tttctttgtt tttgtctcga cgagcaattg ctggcaagat catacctaat
     6361 aagatcaaga caattggtgt gatgatattc gtgaataatt ggaaccacca tgctttagga
     6421 tcagcagtat aatctacttt tggcaccatt cctaggatac atgcaaacgc agtgaaggca
     6481 aagcaccaag caccaaagat aaaaccgatt tttggatttt taacaaattt atattcagaa
     6541 ttgaatttct tataagcttt attcaacatc atatatgcca agaacaccca taggtaacgc
     6601 attggcatga caactgagtt caagttcgtc aaccatttta ctaattcatt catatctttg
     6661 ataccaaaga tcggcaacaa aataatgatt cctactagta tacctgtcag aagataacca
     6721 ttcaccaatg tacctttacg attacgttta cgtagccagt ttggtacaaa ttctggatcc
     6781 gcatctgcta acaagatttt tagtggtgca tcaattgaaa aggctaatgc ggagatttgt
     6841 ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc atagtaatta
     6901 cctaaaattt gaaaagcact gtatgcccca tttgccatca agtctttagg aatattatcg
     6961 ctagcaaaca gcatacccat tgcgactgag cctaatacag cacaaatccc taccattgct
     7021 gctaagaaga tcatcccttt aggaaattcc tttgcaggat ttcttgtaga gttgacataa
     7081 ggggatattt tttccgctcc tccaactgca aatactagca tcgaaacggt tgtgaaataa
     7141 ctgaaatcaa atgttggaat atatgttttc acttgattca tatgtggtgt ggcaaattcc
     7201 atcgttggat tgatgaacgg tgccgttaca gctaaaagaa taaataagat cgacatgacg
     7261 aacatcgctg ttccggccag tccgccaatc actttcaaag tagaaagtcc ttttgttgat
     7321 aaatataaaa aggcaagaaa tataataagt gaaataactg ctaccatttg tactgacatc
     7381 gtatttacga tatttccatt tccttggact gcccaaccgc cagcaatcag gattgcttga
     7441 ggcttttgtg ccaaataagg aatatggacg acccagtaag tccaagcggc atagtatgcc
     7501 agtcgtttat tagatgtcgt attttcgacc catgaactga cgcctccttt actatccttg
     7561 aaggctgacc ctaattgtcc aacgatcaat gcgtatggaa taaaataaat ggccaaaatc
     7621 aatagccaag aggtgacaac tgatactcct tgctgagcat agttattaac tacattcccc
     7681 atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg
     7741 ttcattcgtt ctctccttta tgataaaaat ttgttatcca gatgtaatca tattcaattt
     7801 ttaacaaact tacaagacag tgttatttta tgtgtgtatt cttttattta atacacatcg
     7861 ttttttcata ttttttccat caaaaaaata agttattttc ttttatttca tttaccttat
     7921 tgaatttcaa gctattttga attttctgca aaattaa
//
LOCUS       DAGCYL010000129         7887 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.131, whole genome shotgun sequence.
ACCESSION   DAGCYL010000129 DAGCYL010000000
VERSION     DAGCYL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.131"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1519)
                     /locus_tag="MB216_002112"
     CDS             complement(<1..1519)
                     /locus_tag="MB216_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526910.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLY"
     gene            complement(1539..1742)
                     /locus_tag="MB216_002113"
     CDS             complement(1539..1742)
                     /locus_tag="MB216_002113"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526911.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            complement(1743..2432)
                     /locus_tag="MB216_002114"
     CDS             complement(1743..2432)
                     /locus_tag="MB216_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HBT4526912.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            complement(2687..3007)
                     /locus_tag="MB216_002115"
     CDS             complement(2687..3007)
                     /locus_tag="MB216_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526913.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(3040..4158)
                     /locus_tag="MB216_002116"
     CDS             complement(3040..4158)
                     /locus_tag="MB216_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526914.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            complement(4187..4981)
                     /locus_tag="MB216_002117"
     CDS             complement(4187..4981)
                     /locus_tag="MB216_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526915.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(4991..5866)
                     /locus_tag="MB216_002118"
     CDS             complement(4991..5866)
                     /locus_tag="MB216_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526916.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            complement(6083..6340)
                     /locus_tag="MB216_002119"
     CDS             complement(6083..6340)
                     /locus_tag="MB216_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526917.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            complement(6343..6567)
                     /locus_tag="MB216_002120"
     CDS             complement(6343..6567)
                     /locus_tag="MB216_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526918.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(6740..7783)
                     /locus_tag="MB216_002121"
     CDS             complement(6740..7783)
                     /locus_tag="MB216_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBT4526919.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         2207 a         1629 c          994 g         3057 t
ORIGIN      
        1 aatatagacc atgattcaag taatctgtat atagaaaaat cgcatcacac gtattcaaat
       61 ttttaagtat ctttccatta ttttcgctta catcaaacat tttaatattt ggtaatagtt
      121 cagttaattt attttgaaaa tttacatgtc ctcccactaa aagaatgttt ttatgtttta
      181 gataagctat catttcagac agtgaaggct cttcagtttt accatttaat gattgtctat
      241 gttcttgctc ctgcaattgc tttaatactt tattttcaaa cctcagctgt tcaagttctt
      301 ttctgattcc ttttaattct ttaatctctt gcttcaaaag ttcctcttct ttttttgccg
      361 atttgactaa agattctaac cgaccgtttt ctttactcgc aatacttaat tcttttctta
      421 atttgattac ttcagatttg ttttcttttc taatacttgc aatttgtttt gctatttcat
      481 aatcagaatt caggatcaat tcttccttag catttttaaa caaccgagtt aaaacgaact
      541 gatataacaa actataaaaa actagcaaat agtcaatttg tcggtactcg gtattctgat
      601 caattgcata tagagagatt aaaacgaaat acacaatatc attcagctcg ctacccttga
      661 tctcaaaatt attgactaag taactatcaa tacccatcaa atctgcaaaa tctccataca
      721 aagaaaaata tgattttgac aatatatcta tatctgaatc gtcaaaccca tttactattt
      781 tcccatcatt gttagtagtt gctaacgaag caatagtgac ttttgaattg ggttttacaa
      841 atcctatacc taatagttct ttcatctttt taatttcaag tttgttatcc ttttgttcgg
      901 ctttatgctg tttcatattg taaagcggat aaaatgctgt tccgtaagca ccttttagta
      961 cgttatctat aaaataatct tcagaacctg ttttgaatag aaagttaatc gcagccaaat
     1021 tagataacag ctcttcagtg ttgtgcatca attttttatc atttaataaa ttatccccaa
     1081 gatatttcat tgcataataa gggtttgttt cgctacttat ttttaatatt ttttgatagc
     1141 tattcctcac gatattaacc aaaatatctg tctgattaat tgaattacag tacaaagcca
     1201 ctcctaatgc catataaaca ttcaaatcat gttttgccgt accgttgaat aaaacgtctt
     1261 caacttttga attatctcgt ctatactttt tgatcatatt tttgatattt tgatcattct
     1321 cttggtatat ttttttcact cgttccaatg ccttgggttt gttggaaaca gcaacagcga
     1381 agtctagttc tttgaaatca atatttagta tctgtacctc atcacctaga ttataatctc
     1441 tatttacgct cggaaaactg ctgaaatcta actgtttttt acctattttt ggcgaaaata
     1501 ttccttcgat ttctggcaaa tgtaatcaca ctctctcctt atttatcttt agaaaatttg
     1561 tcactattct ccttatattt atcaataaac ctttttaatc gagcaatata agcgttaacc
     1621 cttttaggtt cttcttttcg ccattcatcg tttacgtgac cattttcaaa gaaagaagct
     1681 aaaagagttt ccacttcatt aaccatatct gcgatcgtat atttttctgt accctcactc
     1741 aattattcat gaactgttaa gtgttcatag ctaagttcat tcataatttt gttatagcgt
     1801 tttcttgctt tttcaaattc tttctgttcg tcaaattcca cttgattctt catgttgttc
     1861 ttcctctctg tttgatattt tttcacttct tcaagtttag catctagttc cgctttaact
     1921 tctgtcactt ctttaagcgc ttgggataac ttatgagaca atttcgcata agtttctctc
     1981 ttgtattcat ttcctactcc caactcttcg gagtagaact cttctagtag tcttgcaaaa
     2041 ttaacatttc tcctgccagc atagtttttt agcttctcaa aatcagattg aaagacagca
     2101 acacttttgt aatccttttc accctcaaaa tcaaaagcat agtcatactt tcctgaaatc
     2161 aattcatcaa taactgtagc aatatattga gagatagaaa tattatcttc acttctatgt
     2221 cgctctataa gattatatat atcagggctg acatgaaacg tacttctctt ttttgtttgc
     2281 ctttttctaa ctgttacgac cacttttgga acttcactat ctccattgat ctctttgtat
     2341 ttttcaataa ataattttct agcttgttct cgactttttg ccggaatttc atctacaatt
     2401 ttatcttcta aataaatata gtgtgttctc atacattcgt cctctatctt ctttactctc
     2461 aatcttcatg atcttagttc ttaccttgtt tactcttaaa cattgcaaaa aaatctagtc
     2521 aaaatcaaac ttgaaatcat cgtctatcac aacaccctta acttcatctt tagatttccc
     2581 aaaggaaccc ttattttcaa atcctttatt tttgaatgat gatggttatg gaaaacttcc
     2641 tttgaaattc tgacaagcat aactagtcag aactgtttta cttagattaa gctttcaaaa
     2701 gatactgatg aagaactaag ttctcagtac cttcccattc ttcaataaaa aatatttctc
     2761 cttcttttac ccactctata gctaatttct tataatcaaa taaaccatat gtgtttaagc
     2821 ctttgtcttt cattaaactc aaaaactgat ccccttttaa tctttctgta tcatccatca
     2881 gaaattcaac taaatcttta tccatgcgtg caactttttc gtcttttagt gccattgatt
     2941 tagcatagtc agttgaatct tttagaataa taacacctac atttccttcc acaattttct
     3001 tcttcatatt tacctcctac catcttttcc aaagagtatt tagtcaaaat caaactcgaa
     3061 atcatcgtct atcacaacac ccttaacttc atctttagat ttcccaaagg agcccttatt
     3121 ttcaaatcct ttgtttttaa attctaaagc tttttcgact tcttctttgg atattgtatg
     3181 aacttttttc ttaactactc caagtttata cagctcttca aactctggta cttcggatct
     3241 gaaatacaat ccatcagtac gaatttttaa tagcccatat gcgctatgaa cgtatttctg
     3301 ttcatatcta accatacgat ctctaaatac gcctgctacc atggaggaat acgctcgatt
     3361 atacatactg tatttatcga aggtgcttgt ttctccttct cttaatgatt tttgtccgat
     3421 agcaccaaat actttcgttc taagattttt ataaagcttc aacgtatcag gatcagtttt
     3481 taatggctcg attctttcaa aatattcttt gagtaatcct tttttcgtca aaaatgtgtg
     3541 gctatctagg acttccatct ctttaatatc ataataatca aggataagtg ttagctcaat
     3601 ctccgataac caaataggct cattagctgt tttgggattt tcgccataat ccgcaaacat
     3661 cgactgtgat tttggattag catatgttcc ttctttggct gttccttcaa aattaaccaa
     3721 aaagaacata tacgttttta aattttcata cggtcttact tttggtctat ttatcgctct
     3781 caaaatagct tgatctgtat tatattttgc tttcaaataa gtgtttgacg gcaaaggata
     3841 atttctcatg atcctcgaat aggcttcgtt gaaatcatat tcgatcatct ttgctccttc
     3901 ctcaaacaaa tccaagttat acatgctacc ggaggtactg gctctatagg ctgaacttgg
     3961 aacaaagaaa ggtgcatctt ccggcttatc atagcccaaa aaatgtattc ctgtttgctt
     4021 gttctgacgg taaatgtcca tcaaaagata tttgatcgta ctaaaagaat ctaattttcc
     4081 tcttgtcatc caccctaatc cgacaagtct tttaatttta ttctgcattt caggtgtgta
     4141 tatgctattg tttcccattt atctgctcat ctccggcttt tttattctag accctatatt
     4201 tgttcattac accacttatc tcgtctacaa aggctcccag atcgtgtgta aaggcatcat
     4261 agtaatcttc aatctcactc gattcagttc tccggtttct tccgttttta tttctgaata
     4321 gatacatctg cgtttctctt aaatcccttg cttttttcgc aagtgctaat aaatccgtaa
     4381 aattatcaat aaccataatg tcattgatca taaaaccttc ttcatgacca tacttttcag
     4441 ccatctcgat aaaatcatta acatcgaaca actccaataa ttctgcttta tatttctcta
     4501 acaatgtatt taaatagcga tttctcaatt cccatgaact ttcaccagtt ccgtattcaa
     4561 ccttgatccc taaattctta gtgtagcctt ttgctgtgta tcgtccaatg aagtctccct
     4621 tattccgatt gctatcttct tctttgaaaa actcacgcca aatccccttt ttcttcttag
     4681 gaattagacc tctgctcttg aaatcatcat atttttttga agcttctctt cgtgtatcta
     4741 agtacctttg cttcagctct gtagctgatt cattcgttcc atattcaaca gtgatcctta
     4801 agcgcctgct ataacctttt gctgtatatc gtccaacaaa gtcaccgcca cttcgatcac
     4861 tactgacttc cttaacaaat tctctccata tacctttttt gtcctttggt attaatcctc
     4921 gtcttttcat atcttgatat ttttcagaag cttcttttag agtattcttt tttgctacca
     4981 tatattccac tcactctttg ttatctagta ttttaataac tggtttcatt tttgtaatat
     5041 cttctttttc agctatatac aactccgtta cggtaacgat cttagtatga tttagattaa
     5101 ttactttcac ttgatcgcca attttgattt ttcctcgcaa ccgtttggga ataaaccagt
     5161 tgtataatcg tgatttatta ttatctacat aagcttttat tgcattttca ggaaacaggt
     5221 tatttagtct ttcaattttt ttacttttaa aaaattcatt cgcaaacttt tcatcaacaa
     5281 ctaaattaaa taaagtttca gaatttacag cttccatatc tattcctttg tagataggag
     5341 aaagcaattt tttatatttt gccagtaatg actttccaga attactaatc ttaatttggt
     5401 tacttaaatc ttcttgataa agaaccccat atcgaagtaa cgccttaata taatgtttta
     5461 atatgccgtt gataccagaa taaatatcca tgccgttctc aatatagtag aaagtgtcgt
     5521 ctctccaaaa caatttgaag ctattattca aataaagtaa caagatcaat atatgtttgt
     5581 cagaaattct gacaaatcta ttacttttta gatactcacc aatggcttca cttaactctt
     5641 tgaagccatt gtattttccc gataaaaatg tcgtaacatt atcttctctc tttttcctat
     5701 ggtgtgaact taccctgaaa taatagagtc tattctcatt taatattgaa aaataaatac
     5761 tttcagttgt ttgtgagcat tgtatatagt tgaaatgata tccatctggc atgacttttt
     5821 taactgtatt ttgtactagc tctgaccacc ttttgttagt agtcatatat ttttttccat
     5881 taaaccaaac ataaatatct tttttaaaaa ttttgttccc cccctattgc gttttctttg
     5941 acattaaagc cctttgtttt catcacattc gtatagccct agccactcat aacctgatgc
     6001 ttttgcgaag tttatttaaa agtgttttcc tttgtaatct acctttcccc tcgtcctggt
     6061 acttttcttg gcgactatta aactattcaa cttttttaga atcattgtca gcttgaaatc
     6121 cttcacgttt cattatgtct tctgcttttc caatgctgat tcctttccaa tcacgccatc
     6181 ctacgcttcc cttattttga cgataggctt taatagctga ttcggatatt tgccatcttg
     6241 tagccaactc tttggctgtg tacttatttg aaaaaagtag cttctctatc agtgaaatat
     6301 caatcatttt attctcactc ctgaaatctt cattaatcat ctctagctag ctatatcttc
     6361 aatcaattct aatatgtcgg ataaaccatc aacagcttct tcaagttgat caacggattc
     6421 ttctgctttt tcatatcttt ctgatccctg tagattttta ggtatatttt ctttgtactc
     6481 ttcttcttga attacttcta actcatcctt tagctcggaa agtcgctcgc ttatttcttc
     6541 taaccttttt cttcttcttt ggttcatcaa aatcacctct tcgcataatc tctacgattt
     6601 taatatacca cttttgtgta ctacattcaa tattttttgt tatagattcg tattacaaaa
     6661 atttcctcta aaaaaataag agcctattta ccatttcagt aattaagctc ttatttcatt
     6721 tagttgataa ttctatcaat taacgtacag caaccgccat tccagtcaca ttaaatgctc
     6781 tctcatacgt gcttgcattg atccattttg cgccaaatac agggtcaaca acatggtatg
     6841 caccatttct gaagccatcc acgagaacta catgtccatt ccatactgca cgaccccaag
     6901 gcatttgttt gaactctggc gtagcatagt tataagtaat ccaagttaca accggattac
     6961 cattgcgaat ttcattcttg attccattcg ttcctgaacc agtgatattg acggcattac
     7021 cgccaacgtt ttttgcccat ttaactactg ggtcagccat catgccttga tacgttccat
     7081 taacgttatg acgccactct cctgagtaac catgataagg attattgtca ttagcttttg
     7141 gcatatagtt tacaaaactg gttaagcttt gatttgtggc tacacctttg tagtgtaaac
     7201 cttctaataa agaagctcct tcgcataaca ttgtatttcc ttgccataca acaggaacgt
     7261 tcaacacttt ttcagtgctt ggagcaggtg taggatttgg tacgctgttt ggcattgcat
     7321 accagcctgt accttcaatc ttccaacctt gtttactaat tacgttcttt tcattttgat
     7381 ctaacgtgta gtgatgccaa ttcacgtatt tgttatataa acgtgttaca ggaactctat
     7441 ttgctccgtt gtaggaatag aacgcaacac cttcgtacgt ccatcctgtg tgtgaaatct
     7501 tatctctttc tccggcatca actgtgtaaa aatgttcacc gttataccga ttatacaaac
     7561 gataaacagg agtaccactt gttggagctt gccatgcgac accttcgtaa tgccatccat
     7621 aacgtttcag attgtctctt tctccaacgc ttctagtgta tagatgctct ccgctattgg
     7681 gattatacat acgatacatg gaaatagagc tttctgctga aactgaaggt gataataata
     7741 ctagccccat tgtgaataga attcctacta aaaatttttt catttgattt tacccctttt
     7801 cattttttat tgtcattatt gcatgaaaat tattcgtaag attgccaatt acataaacga
     7861 ttgttttaag atcataatcg ttgttgt
//
LOCUS       DAGCYL010000130         7879 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.132, whole genome shotgun sequence.
ACCESSION   DAGCYL010000130 DAGCYL010000000
VERSION     DAGCYL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.132"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..197
                     /locus_tag="MB216_002122"
     CDS             <1..197
                     /locus_tag="MB216_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526920.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="MB216_002123"
     CDS             314..811
                     /locus_tag="MB216_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBT4526921.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="MB216_002124"
     CDS             898..1290
                     /locus_tag="MB216_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4526922.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..3721
                     /locus_tag="MB216_002125"
     CDS             1274..3721
                     /locus_tag="MB216_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4526923.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3724..5907
                     /locus_tag="MB216_002126"
     CDS             3724..5907
                     /locus_tag="MB216_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBT4526924.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5904..6905
                     /locus_tag="MB216_002127"
     CDS             5904..6905
                     /locus_tag="MB216_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBT4526925.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6920..7834
                     /locus_tag="MB216_002128"
     CDS             6920..7834
                     /locus_tag="MB216_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4526926.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
BASE COUNT         2454 a         1521 c         1683 g         2221 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     2821 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     2881 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     2941 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3001 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3061 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3121 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3181 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3241 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3301 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3361 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3421 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3481 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3541 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3601 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3661 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3721 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     3781 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     3841 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     3901 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     3961 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4021 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4081 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4141 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4201 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4261 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4321 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4381 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4441 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4501 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4561 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4621 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4681 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4741 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     4801 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     4861 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     4921 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     4981 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5041 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5101 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5161 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5221 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5281 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5341 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5401 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5461 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5521 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5581 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5641 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5701 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     5761 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     5821 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     5881 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     5941 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6001 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6061 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6121 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6181 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6241 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6301 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6361 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6421 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6481 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6541 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6601 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6661 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6721 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     6781 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     6841 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     6901 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     6961 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7021 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7081 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7141 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7201 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7261 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7321 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7381 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7441 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7501 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7561 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7621 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7681 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7741 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     7801 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     7861 ttttgtaatc acgtactct
//
LOCUS       DAGCYL010000131         7637 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.133, whole genome shotgun sequence.
ACCESSION   DAGCYL010000131 DAGCYL010000000
VERSION     DAGCYL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.133"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            264..1571
                     /locus_tag="MB216_002129"
     CDS             264..1571
                     /locus_tag="MB216_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBT4526927.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSESKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            1690..2888
                     /locus_tag="MB216_002130"
                     /pseudo
     CDS             1690..2888
                     /locus_tag="MB216_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            complement(2915..4183)
                     /locus_tag="MB216_002131"
     CDS             complement(2915..4183)
                     /locus_tag="MB216_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBT4526928.1"
                     /translation="MEKQLPSTVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(4346..4993)
                     /locus_tag="MB216_002132"
     CDS             complement(4346..4993)
                     /locus_tag="MB216_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HBT4526929.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(5401..5904)
                     /locus_tag="MB216_002133"
     CDS             complement(5401..5904)
                     /locus_tag="MB216_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4526930.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(6027..6791)
                     /locus_tag="MB216_002134"
     CDS             complement(6027..6791)
                     /locus_tag="MB216_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBT4526931.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DVEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSK
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            6899..7174
                     /locus_tag="MB216_002135"
     CDS             6899..7174
                     /locus_tag="MB216_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBT4526932.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         2288 a         1576 c         1399 g         2374 t
ORIGIN      
        1 aaagaataaa agaggtggat cttatgcatt tgattatgat tttcagttta ttaagtatta
       61 tcagtttatt tgctttcagc gcttcccgct tattagatgc ggaaaaacac agcctattcc
      121 tgtaaaagta cgtaaaaacg accgctaacc ttccataacg tgcgttctta tcctaaagtt
      181 ttttcagaaa cattgaaact ctctcccctc tctgataaaa ttttaatttg tagcgaaaca
      241 aattaattac aaggagatcg gatatgtcca taaaaaaaac acgtctagtc ctattattgg
      301 ctagtctcat tctttgcctt gcttcttttc taccagctgt tgctgtgcaa gctgaagata
      361 cgttcaaagt aaatgcaaaa gcagcttttg ctgtagatgc gcaaagcggt aagattcttt
      421 atgatcaaga tggagaaaaa accatgggga tcgcttcaat cacaaaaatc atcggtcttt
      481 acatcgttct tgatcaagta aaagaaggaa aactatcatg ggatgataaa gtttcgattt
      541 cagattatgc agaaaatttg agtatcaccc cagatttatc gaatgttcct ttgcataaag
      601 aaaacaccta cactgtcaaa gaattattcg actctgcaat catccagtca gccaatgctt
      661 caatggtggc cttggcagaa aaaatctctg gcagcgaatc taaattcact gaacgaatga
      721 aagaacaatt gaaagattgg ggaatcaaag atgcaacgat cgttaatgca tcaggactga
      781 ataactctta tcttggcgaa aaccgaccag aaggtactgg agaaaacgac gaaaatcaaa
      841 tgtctgctca agatgtggcc atcgttgccc gccatttgat cttggatttt ccagaaatac
      901 tggacgtatc tagcactacg acacaaatgt ttggcgaaaa cacgcaatca ccagtggaaa
      961 tggtcaactg gaactggatg ctcccaggat tcgttaatta caaggaaggc gtggacggat
     1021 taaaaactgg tacgactgag cttgcaggtg cttgtttcgt tggtacgatc actaaaaacg
     1081 atcaacggat catcactgtc gtattgaatg cggacggcca tgcggaaaac ccgagtgccc
     1141 gcttcaatga aacagaccgt ttgatggact actgctatga caattggtca gaaaaagagc
     1201 tcggaaaagc taacgcaagt attcctagcc taaaaaccat tgatgtaaaa gacggaaaag
     1261 aatcaacagt tcctgttgtg cttaaaagcc ctgtaaaagt atgggtacga aacgatatgg
     1321 atactggcaa attaacgatc acgccaacac ttgacaagaa gcaagtcact gacaacgaga
     1381 tccaagcacc tatggataaa ggtacaaaaa tcggtaccgc tacgatcacc ttggcagacg
     1441 ataaattagg ctatttagaa gacaacaatg cgccatcaac agagatcatt acggataaaa
     1501 cagtggaaaa agcgaatatg ttcgtgattg gctggcgacg cgtcgcagaa tttttcggca
     1561 acttgtttta attgaacaat aagaaaagca tctttccatc ggaaagatgt ttttttgctt
     1621 ttactagttt aaataacagc ttttttcatc tattttcgtt atactgtata agaaaaagga
     1681 gttgaattta tgaatctaac cagagatctg atctttttag ttctctatct tattggggta
     1741 ttctctatcg tttattggcg aaaacagaag aaggatcctt ccttgttctt ttacggcagt
     1801 acgtggacat ttacggtaat cttaacttat tttgtcgtgt tggaaatttt ttattcgacc
     1861 ttttattttt tgattccatt gttgttcttt agtatctttg ctttttctta tttcacagaa
     1921 aaacgaaggc tgctgaatgg tcttttattc aatgttttcc tcatcagttt cggcatctac
     1981 ttgtttgtct tgctgtatga aacgcaaaat attttccttg gcggactgat cgccttgatc
     2041 acgattccat tgctacttgt ccttttattt ggtatttatg gattgattgt ttttcttttt
     2101 tggaacgggg tcacagtttt acgtagagaa tcgcattcat tagctaatct tttaacgttg
     2161 atactagcca tttttcttac actgtttctt gtttttgact ttttcttgtt aaaatatttg
     2221 ccgcaatgga taaatgcttt gtttttctgc gttcccctga tcttgatcta tctatttatc
     2281 gtattctaca actttttgac tgtctcattc ttgtatcagt tcaaccggcc tcgatacaat
     2341 caagacttca tcgttgtgtt aggcgctggg ttgatcaatg gagaaacagt ttctcctctt
     2401 ctagccaagc gaatcaataa ggcaattgct ttttatcggg ctcaaagccg tgctacatta
     2461 aatccgccca tcttattgat gtctggcggg caaggagcag acgaaaaagt accagaggcc
     2521 attgcgatga aacaatatgc catggaacaa ggaattcccg aaagagacat tcttgtcgaa
     2581 accaattcga caaccacttt ggagaacatg ctctattcaa aggaaatcat ggatcagcaa
     2641 atgaaagggc cataccgcgc gatcttcagt tccaacaatt accacatttt tagagccggt
     2701 ctttatgcgc gacaagccaa attgaaagca aatggtattg gtgcaaaaac agctttctac
     2761 tacttgccta atgcgttttg agagaataca tcgccatctt gatgatgaac aaaaaaagac
     2821 atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg attactttac
     2881 ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt tctttcattc
     2941 tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctga gcagaataaa
     3001 taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag tatactgccg
     3061 cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta tttgtcgcac
     3121 cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca tggagcaaag
     3181 gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact tctccccctt
     3241 gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa tcatagacat
     3301 gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga taacgttgtg
     3361 tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt cgccaaacag
     3421 gatgaggaat ccattgtgca taccgtgctt ttaaaaagac gatcgaacgg ctgaataccc
     3481 agccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc aacatactcc
     3541 attcagggat cacacccatt tggtaatgta ggtgagaaac accatagctt tctgtcataa
     3601 aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg actttcccga
     3661 ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat acagaactaa
     3721 aacccgcact gattccactg ataatcaaga tttcccgttc tgctttatcc aatcggaaca
     3781 aacgtgctgt attatctgct aacgctcccc ccatttggac tgctgttcct tcacggccaa
     3841 cagatccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgga ataagtcgca
     3901 acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt ccacgactag
     3961 aaagaccgcc gtgatacgca tagagataag caaaaagcgc accactaact ggtagtagat
     4021 aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta tttaagaaaa
     4081 aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc cacttcagca
     4141 tgtacactaa aatgcgtact gtactcggta attgtttttc cataaaaaat tcctcctcaa
     4201 taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg tcattagcta
     4261 tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta aaagatagca
     4321 tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca gcaaattcat
     4381 agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga caaatataat
     4441 ttcccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga aaaagcgaaa
     4501 cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt gataaagtac
     4561 cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc tgcgtacctg
     4621 gatcattcca gccgatgtaa gtatgttctt gcaagtcgat ttcattccaa gctggttgtt
     4681 tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctaggc tgcataaacg
     4741 gctgaacgat ttgcttcagc tcctccaatg ctctttctgc ttgcgctttt gtcagtttta
     4801 cgtctaaaac tgtcgttaaa aatgtatcga tttctggcag ttcgtgatct aatatgtctc
     4861 gaatcttttc agctgctagt gcctgaactg tttgtaccgt ttttggatca ttgacagatg
     4921 agtacgcatt taataactgt tctacttctc tttttataaa aaaatattgg tatggtttta
     4981 ttgtttgttc catgatttta aagtcctttc ttctaaccaa agtgcatact ttgtagaaaa
     5041 aagtgatctt ctgcaaagta tgtgttacct gaagatcccg gactctttta aataagatac
     5101 gttttgcatc ttatcatcac cccctttaat gcttattatt ctatgtacat gattcattcg
     5161 ataaataaaa gtaaaccgta tcttgaacag atagtcaact gcaaacagaa aaaaggactg
     5221 tgacaaaagt cgtgtcaaag tcccttgttt cgaatgaatg gtgttcaaaa gcttgcatct
     5281 gcggtaataa gccgaaactt cacaaaaatt tgagaagtaa ttttcgtaaa ttccttctta
     5341 tttcttgaag ctgaccactt ctgtcacaac ctcaatatgg tctattattg gttttacctg
     5401 ttaaatcgag gattctttcc caaagtaacg ttgccatctc ttacgcatac ttgcagacat
     5461 gggctgagcc gcttctttga ctgcgcagta tttgtccacg aatgtcacaa tatatccttc
     5521 tttatactta ggcggtgcga tcgttgcacc ccacatgtgc ttcaaaatga tatctcgctc
     5581 aagatccgac aaatcggtga ttttttctgc attctttaca gcaattcttg gatggatata
     5641 cgcatgtgtc ccctcgtcaa atttcgtcgt acgccaatca tagtagaaca gatcatgcaa
     5701 aagtccagca cgcgctgttg ctttagcatt gccaccccat ttttttgcga gcagataact
     5761 atagtaagaa acactgatgg aatgctccaa acgagtggaa tgaacatgct gtgtgtaatt
     5821 tgctaatttt ttcactgctt ctgtttctaa aagatcttgt acataagaca tatattcttc
     5881 gtcttcacgc catttttctg tcataattat cgatctgcca cctttttgaa tttcattttc
     5941 ttctgaaatt gatctaagta taccaccagt gttcggatta ttctagtcgc tctagataaa
     6001 atagctaaag cttaatagtt ccttaatcat aaagaaaaat gataaagaac gatccctgct
     6061 gcgactccga cattcaatga ttccgcattt cctttcattg ggatatatac gttctgatca
     6121 gtcaaagaca agatttcctg acttactccc tgaccttcgt ttcccatgat tatagcgaaa
     6181 tcctctgttt tttctacttt gtctaatgca tgtgcttctt catttaattc tgtaccgtac
     6241 accgtaaacc ctttgccttt cattacaggg atcacttctg ccaaatcttt ttgtaacaca
     6301 ggcaaatgat aattgcttcc ctgcatgctt cgcagcacct ttgtactata aatatctgcc
     6361 gttcctttac ctaataaaac accagccata ccaaacgcat ctgccgtacg gatcatcgtt
     6421 cccacatttc caggatcctg tacattatct agtaaaagcc agcctcctga aaaatctggc
     6481 gtatctttag atgttaacgt catcactgct aagatccctt gcggtgtagg tagatctgac
     6541 atagcggtca tgacctcatc tgaaactaga aaagtagaaa tctctttgtt tacagccagc
     6601 cagtctcccc attctttcat acctctctgt gtcacaaaaa gttcttttat ttctacatca
     6661 gacgacgcag cttcttcgat cagatggaac ccttccaaaa gatattctgc cttttcttct
     6721 ctgtatttct ttttatgtag tttttttaat tctttgattc ttggattttt tgctgattgg
     6781 atctctttca cattccttca cctcacctga ttatatcatg gaataggtgc ttgagcttgt
     6841 ttcatcctaa aaaaatcggt atcattgatt tataaaacag gaaagaaagg tgacgaacat
     6901 gcgcaaagtt aaaatgaatg tccaaggacg tgtccagggc gtgggatttc gctatatgac
     6961 caagatggtt gctgatcagt taggcgtgac cggaacggta aaaaacgaag acgacggctc
     7021 agtcacaatc gaagcaatcg gaaatgacga catcatccaa aaatttatcg aagaagtcaa
     7081 gaagtctccg agcccaagtg gacgagtgca gtatgtggat gtccaagagg atccgatgat
     7141 tgaggaacga aagaaatttg atgtggtggg ataaggttgt gaaaaggtcg ctgagttttg
     7201 agtattaagg aaaattttgt gaaaatagct ttccacattt ttgcataaat ttgacttaat
     7261 accgagaaac tgacttttga acaccgtcga tcaggttgcg aaaaagctga tctgcatcaa
     7321 gcattaagga ctggttgtga aaaagtcgct tagttttgag tattaagaaa aattttgtga
     7381 aaatggcttt ccacattttt gcataaattt gacttaatac caagaaattg acttttgagc
     7441 accgtcgatc aggttgtttt tgaggcgctt tgcctcaagt attaaagata ggtagtaaaa
     7501 aagctgatct ataacttgca ataaatttgc ttaacccata ttttccagac agaacaaaaa
     7561 agtttttagt tttttaaata agaaagaaac acttggaatc actcttttct ttccaagtat
     7621 ttctttcttg atttttt
//
LOCUS       DAGCYL010000132         7637 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.134, whole genome shotgun sequence.
ACCESSION   DAGCYL010000132 DAGCYL010000000
VERSION     DAGCYL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.134"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            371..3178
                     /locus_tag="MB216_002136"
     CDS             371..3178
                     /locus_tag="MB216_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBT4526933.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLGTFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3369..3860
                     /locus_tag="MB216_002137"
     CDS             3369..3860
                     /locus_tag="MB216_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4526934.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3963..4952
                     /gene="manX"
                     /locus_tag="MB216_002138"
     CDS             3963..4952
                     /gene="manX"
                     /locus_tag="MB216_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBT4526935.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4985..5788
                     /locus_tag="MB216_002139"
     CDS             4985..5788
                     /locus_tag="MB216_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBT4526936.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5809..6723
                     /locus_tag="MB216_002140"
     CDS             5809..6723
                     /locus_tag="MB216_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBT4526937.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6806..7180
                     /locus_tag="MB216_002141"
     CDS             6806..7180
                     /locus_tag="MB216_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBT4526938.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7319..7564
                     /locus_tag="MB216_002142"
     CDS             7319..7564
                     /locus_tag="MB216_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBT4526939.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
BASE COUNT         2475 a         1263 c         1683 g         2216 t
ORIGIN      
        1 ataaaagttg tagtggaggc tacgaagttt actttgtctt tttacactta atattttttt
       61 gccaatttgg aatcaggatg ttttgataac tatcgttttc aattgatgga agctatattg
      121 tttgctatca gtgaaataat tatatggtaa acagtgttct ttttcttgtt tccattataa
      181 aaatcgtatc gggatttttc gttcttttaa attgaacatt tctatctatc cgcgtgttat
      241 caacaaaaca aaatcaggta aatagttgac gaaacagaaa taaatacata gtattatgtt
      301 ttgaagccta agtacaatag tctatagaaa aggagcgata ccattggcaa aacgcattga
      361 ccgaatttat atgtatgtca aagaaaaaac cgctcattta acaaaggcag aatatgatca
      421 aggagtaaca acacaggaaa ttgctgaagt gttagggatt cagcgaacaa atagcagtaa
      481 agatctgaat cagctagtgc gcgaaggaaa actgataaag acagacggac gaccagtacg
      541 ttacatatat caaacactcg ttacgcacgt aaagccagtt acaaaacatg tagtcagtta
      601 caaagaaacg ccggtctctg aagaaaaaaa tacactccaa atagatacta aggatatctt
      661 tgcaaaaatc attggagcta acggcagcat gaaaaattca gtggaacagg caaaagctgc
      721 gattttgtat ccgccaagag gattaaattg tttgattaca ggacctaccg gatcaggaaa
      781 gacttatttt gcacacgcga tgtatcattt tgcaaaagcg catcatgtaa tagatgagga
      841 aacggaactg attgttttca attgtgctga ttatgccaat aaccctgagc ttttgatgag
      901 tcatttattt gggtacgcaa aaggtgcttt tacaggcgca gatgaagaaa aaatggggat
      961 tatcgatcaa gcggatggcg gtatgctgtt tttggatgag attcaccggt tgccgcctga
     1021 aggtcaagaa atgatctttt actttatgga tcatggaact tacagtcgtc ttggggaaac
     1081 aacgaaatcc catgaagcaa atgtacggat cgtaggtgcg accacagaag acccaggttc
     1141 ttctctatta ggaacttttg ttcggcgtat cccaatcaat atcaaacttc cggcttttga
     1201 aaaacgtcct gcaaatgaga aagtcgattt agtaaaaatc atgattgccc acgaagcaaa
     1261 ccgcacgcag cgaaagattt ctttgacaga agatgtagca aaagcattga tcggcagtgt
     1321 gacctatgga aatataggac aattgaagtc aaatatccag ttagtatgtg cacgaggatt
     1381 tctaaatcat atgcaatcac ccgaaatctc gatcacaatc gatgatttga cagaagggat
     1441 tcgtagcgga ttgattcaat tagcaagcaa ccgcagtgca atggcagagc tttctaaaat
     1501 attggagcca aagataactg tagcaccaaa tgatacgatc atgaaaatcc aatcggactc
     1561 ctatgagtta ccttataatc tatatgatat catcggagat aaagcagctt tactgaagtc
     1621 ggatggtttg gatcaagaag caatcaatca tttcatttct acagatatca acatccatct
     1681 gaagtctttc tataaagacc atggtttttc ttttaatgca gacagcaaat tagcagaatt
     1741 tgtagatccg aaaatcatag aagtgaccaa tcagatttat gtaatggtca aaagatcttt
     1801 accatatgag tttcagcaaa actttattta tgctatgagc ttgcatatca gttctttttt
     1861 gaaaaaaatc aacttaggag aagaacggca tacgaatgat aacatccgtg agatggcttt
     1921 tgattttcca gatgaatatg ctgttgcgaa agaagtgcgt agttatatcg aaacgtattt
     1981 tcaagtggag attccagaaa gtgaagacta ttacttgacc gttctactcg tctcgttacg
     2041 ggcaaatcaa gcaagcggca gaatcggtgt agtcgttgca gctcacggaa acagcacagc
     2101 aagcagtatg gtccaagttg tccaacagtt gttagatgca gagaacgtac gagcagtaga
     2161 catgcctttg gatatggatc cgaaaacagc attggaccga attgaacgca atgtacaaga
     2221 agtagatgaa ggaagcgggg tcatactctt agtagatatg gggtcccttg cgtcgtttaa
     2281 ttcgcaaatt caaagagaca ctggaattcc agtacgtact gttgatatgg taacaacttc
     2341 tctagttttg gaaactgtac gaaaggtctc agtattaggg acagatctag atatgttgca
     2401 tgaatcattg aaaaattttc gaggttacgc agaaatatct tctgtagaat ctgttcagga
     2461 agcaaaacct attcataaga aaaaagctgt tttggctatc tgcgcatctg gcgaaggtac
     2521 agcacaaaga atcaaagaat tgatcgaacg agcggtaaac aagcgtcaag aaacagaatt
     2581 agaagttcta gctttgtcga ttgtggaagt gaaagaagaa ctgccagtta tccaaaaaga
     2641 atatcagatc atagctacta ctgggattac tgatttgaat atccaagccc catttattcc
     2701 tttagaacgt tttattgatc aaaatatcga agtcattttg gatcagttgc tgatggaatc
     2761 agaattggag gaaactgaat tcatctccct agatgaagag tcagctaaaa atacgtgtgt
     2821 tgagtttatc tctgataact tcatctttat taatggtagt aagttaatcg atccaatgtg
     2881 gcaattttct acacaaatct ctcagacgac gggaatcggc gatgaagaat atggattcaa
     2941 aatcaaccta gtgatgcaca cagcaggaat gatcgaacgt attattcgga atgaaccatt
     3001 gactgtggaa gaaaacgaat tgacaaatac aacgaatgat cctctttata gccaattggc
     3061 agcctcagtt gttctgttgg aagatcagat aaaagtaaaa gtgccgatag aagaaatgta
     3121 ttatctgcta cgtctagtcc ataatcagtt agataaaaaa gaatacactg tgccatgaat
     3181 acacaaaaac gatacacttt tatagtgtat cgtttttgtg tattggttaa gaatccttta
     3241 aattcaatta gtgtatcgta aaaaagttgg cacgcttttt gcttatagta gtgtgtgcta
     3301 ggaggtggtt gttcacacag aaatgatagg ggtttctgtg aagtagtgag gaagaaggag
     3361 ttttgcagat ggatattcga ttggcacgta tagatgatcg tttaatccac gggcaagtcg
     3421 caacagtttg gtcgaagcaa acaggtgttg aacgtatcat tgtgatcagc gatgtagtcg
     3481 ctcaagataa attgagaaag tttctactaa aagaagctgc accgccgggg atcacagcaa
     3541 atgtaataac cgtgcagaaa atgattcaaa tctatcatag tggattatta tcacgagcaa
     3601 aagtgatgct tttattcacc aatccccaag atgttgaagc ggttgtccgc tgcggtgtac
     3661 agttgaagtc gttgaatatc ggcggtatga gttttactga aggcaaatca atgattacca
     3721 attttgtttc agtaaatcaa caagatattg attcctttcg ttacttgaac agtcaagaag
     3781 tggagttaga gattcgaaaa gtacctgctg acaggaaaat ccttttgatg gaactactag
     3841 cgaaaatcaa aaaggtgtag caagaatggt tgttggaaaa ggtcaaataa ttctgttgta
     3901 atggttagca attattaata tttttgaatt tgttacaaca aaagtaaata ggaggtataa
     3961 atatggtagg gattatccta gcaagtcatg gacaatttgc tgaaggaatc ttacaatccg
     4021 gttcaatgat ctttggcgaa caagaaaacg tcaaagccgt gattttgaaa ccaagtgaag
     4081 gtccagatga cttgagagcc aaattggaag aagcggttgc ttcttttgat aatcaagacg
     4141 aagtgttatt cttagttgat ttatggggcg gaacaccgtt caatcaatca aacacattat
     4201 tcgaagagca caaagataaa tgggcaatcg tcagcggttt gaatttacca atgttgattg
     4261 aagcctacgc atcacgcttt tcaatggaat ctgctcatga aattgcagca cacatcattg
     4321 aaacagcaaa agacggtgta aaagtaaaac cggaagaatt agaaccggca gaagcaccga
     4381 aagcagcagt tgaagacgca caacctaaag gagcacttcc agaaggaact gtggtcggag
     4441 acggaaaaat caaatatgta ttagcacgtg ttgactcacg tctgttacat ggacaagttg
     4501 cgacagcttg gacaaaagca gtgcaaccaa accgtattat cgttgtttca gatgcagtat
     4561 caaaggatga tctgcgtaag agactaatcg aacaagctgc tcctccaggt gtaaaagcaa
     4621 atgttattcc tattagcaaa atgatcgaag tagcaaaaga ccctcgtttt ggaaatacca
     4681 aagcattgct tttatttgaa aatcctgaag acgtattgac agctgttgaa ggtggcgtag
     4741 acatcaaaga attaaatgtt gggtcaatgg cgcattcggt cggtaaagtc gttgtaagta
     4801 aagtattgtc tatgggacaa gaagacgttg aagcgtttga gaagcttgaa caaaaaggtg
     4861 tgaaattcga tgtacgtaaa gtacctaatg attcacgcga taacatggat gatattttga
     4921 aaaaagcaaa agctgaatta gcaaaagcgt aataaataat attaaaattt ataggaggct
     4981 tatcatgtct attatttcaa taattttagt cgtattagtt gcctttctag ctggtatgga
     5041 aggtattctt gatgaatttc aatttcatca accactagtg gcatgtacat tgatcggttt
     5101 agttaccggt aacctcgaag ctggtatcgt acttggcggt acgcttcaga tgatcgcttt
     5161 aggttgggca aatatcggtg ccgctgtagc accagatgct gcattagcat cagtagcatc
     5221 agcaattatt ttagttttag gtggacaagg tgttaaaggt gttccttctg cgatcgcgat
     5281 tgccgttcct ttggctgttg cagggttgtt cttaacaatg atcgtacgta caatcgctgt
     5341 tccaatcgtt catttgatgg atgcggctgc tgaagaagga aacatccgta aggttgaaat
     5401 gtggcacatc attgctgtat gtttacaagg ggttcgtatc gcggtcccag cagcagcgtt
     5461 gctatttatc ccagcacaaa ctgtgcaatc attccttgaa tcaatgccag catggttaac
     5521 agatggtatg gctatcggtg ggggaatggt cgtagctgta ggttacgctt tggtaatcaa
     5581 catgatggct acaaaagaag tatggccatt cttcgttatt ggtttcgttg tagctgcaat
     5641 ttctcaatta acattgattg ctctaggtgc tttaggtgtt gcattagctc ttatttactt
     5701 gaacctttct aaaatgggtg gttcatcaaa taatggaggc ggaagcaact ctggtgaccc
     5761 tttaggcgac attttgaatg actattaatc ttgaaggagg aaaataaaat ggcagaagaa
     5821 aaaatcaaat tatcaaaaaa agatcgttta gctgttgcgt ggcgctctac cttcattcaa
     5881 ggttcatgga actacgaacg tatgcaaaac ggtggctggg cattttctat gattccagca
     5941 atcaaaaaat tatacaaaac aaaagaagaa cgttctgcag cattaaaacg tcacttggaa
     6001 ttctttaata cacacccata cattgcttct ccaattttag gggttacatt ggctctggaa
     6061 gaagaacgtg cgaatggcgc gcctgttgat gatgtagcga ttcaaggggt taaagtaggt
     6121 atgatgggtc ctttagctgg tgttggtgac ccagtgttct ggtttacagt acgtccaatg
     6181 ctaggtgcac taggtgcttc attagctatg ggaggaaata tccttggacc aatcatcttc
     6241 ttcttaggtt ggaacattat tcgttgggca ttcatgtggt atacacaaga atttggctac
     6301 aaagctggtt ctaaaattac tgatgacctt tctggcggat tgttacaaga cgtaacaaaa
     6361 ggtgcttcaa ttttagggat gttcgtatta gctgcccttg tacaaagatg ggtatcaatc
     6421 aaattcttac cagttgtatc aactgtaaaa ttagataaag gtgcttacat cgaatgggat
     6481 aaattgcctt caggtggtga aggaatcaag agtgcatttg aacaagttaa taatggctta
     6541 gccctttctc ctgaaaaggt aacaacatta caagacaact tagaccaatt gattcctggt
     6601 ttagctgcat taggattaac attgttatgt atgtggttgc ttaagaaaaa ggtttctcca
     6661 atcgttatca tccttggttt attcgtagtc ggtgttgttg gacacgtcat cggactttta
     6721 taatccataa aaatatagct cgggcgttgt cccgagcttt ttttatatag tatactgttc
     6781 ataaacaaat atctggaggg agaaaatggt tcaatcaatc aatacaaaag tagacttggt
     6841 aattgatgct acttcgttta cgggtctgac agattacggg caaatcatga taggtgataa
     6901 tggttttgag ttttaccatg cgcgagatag acgcaaatat atccaaattc catgggaaga
     6961 agtggattat gtactagcat cagtcatgct aaagggaaaa tggattcctc gatatgccat
     7021 tcaaacgaaa aaaaatggta catacacatt ttcttccaaa gaacctaaaa aagtgcttcg
     7081 tgcgatcaga gaatatgtag atgaagatag aatggtacaa tcattgagtt tctttgatgt
     7141 agtgaaacgt ggtattcgta gtatgtttaa acgcaagtaa aaaataaaga aaacttctaa
     7201 gaggaggttt tctttatttt tttactttgc agaagaaaat atgaaatagc aaaaccaatt
     7261 atttgtcccc tttaaatccc atatgctaaa attgattagc aaagaaggag gttatattat
     7321 gagttggtta tggacattga ttgttggagc cgtgatcgga gcaattgcag gtgcgatcac
     7381 cagtaaagga aattcgatgg gctgtgtaac gaatatcatt gctggactag tcggttcaag
     7441 tattggacaa gcgattttcg gcgaatgggg accaaaagct gcaggtatgg cacttattcc
     7501 atctatttta ggcgcagtga ttttggtagc agttgtctca tttttctttg gaaaacgatc
     7561 ataagttttt acaaaatagt aaactaaaag gagttgcagc gagactgtga ctcctttttg
     7621 gctttattta tttaaaa
//
LOCUS       DAGCYL010000133         7352 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.135, whole genome shotgun sequence.
ACCESSION   DAGCYL010000133 DAGCYL010000000
VERSION     DAGCYL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.135"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            30..809
                     /locus_tag="MB216_002143"
     CDS             30..809
                     /locus_tag="MB216_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526940.1"
                     /translation="MEKPFVKSLTAVAVGAGAIVICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVIQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNISY"
     gene            855..1799
                     /locus_tag="MB216_002144"
     CDS             855..1799
                     /locus_tag="MB216_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBT4526941.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGSDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1815..2594
                     /locus_tag="MB216_002145"
     CDS             1815..2594
                     /locus_tag="MB216_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBT4526942.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKRTAPARPKATNDKTAPSRRSRSRV"
     gene            2606..3304
                     /locus_tag="MB216_002146"
     CDS             2606..3304
                     /locus_tag="MB216_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBT4526943.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3332..4096
                     /locus_tag="MB216_002147"
     CDS             3332..4096
                     /locus_tag="MB216_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBT4526944.1"
                     /translation="MIDLHCHILPGIDDGSENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4110..5929
                     /locus_tag="MB216_002148"
                     /pseudo
     CDS             4110..5929
                     /locus_tag="MB216_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
     gene            5940..6572
                     /locus_tag="MB216_002149"
     CDS             5940..6572
                     /locus_tag="MB216_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBT4526945.1"
                     /translation="MKAIIIGAGGFGKEIAFLLQSVSRYELIGFVDDSLKMKNQELLG
                     KPVLGTIDSLIELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVAPSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDAQIGNYNSIFPSVNISGHVILGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNNTKNVGVPTKVIERWD"
     gene            6574..>7352
                     /locus_tag="MB216_002150"
     CDS             6574..>7352
                     /locus_tag="MB216_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBT4526946.1"
                     /translation="MENKRILLASPHMSEEGYEQEYVKEAFDTNWVAPLGANVNGFEK
                     ELAKYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRY"
BASE COUNT         2586 a         1275 c         1542 g         1949 t
ORIGIN      
        1 atctatcatc aatacttagg agtgaatcta tggaaaagcc attcgtcaag tcattgactg
       61 ctgtggcggt aggtgcaggt gctattgtga tctgtttgtt tggttaccat atcaataatc
      121 agcggcaaca tcagcaacgg atcaattatg cagaatctgc catcaccaat caaaaagaca
      181 ccttgacttc tttatcgaaa gaagtagaca agctgtatag tacaaaagaa aaaatttttc
      241 taaaccctga aataacagaa gaaacaatta caagtttgtc caataagcta tcctctgtca
      301 aactttcagc agatgattac gacatcaaag aatcagaatt gcctaaagaa gctgttgcaa
      361 tccaagatga gaagaaagca atcgtgacac agttagatag tgcagaatca aaactgaaga
      421 tccagacagc tgtcaacgat ttatttacta aaaatgtatc aaactggcag caagctgtag
      481 acgatgtcat catcaaagaa aaggtgacaa gtgcagatgt cgctcgtgta cgtgaaaata
      541 tgagcttttt taaagatagt ccttggaaaa cggttgttat acaatatcta ggattcgctg
      601 atacccagat cgcgcaagtc acgcaactag atcagttatt cgatacgatg ttaaaggatg
      661 gtcaagtgac tgccaccgct acttacgatc aatatttgac ggcgctcagt caaatagagc
      721 agatccggaa tgaaaaaata agcgtagcat acgcaacaaa agcacaaact gttgctcagc
      781 aaatgggcta tagcaatata agctattgac agttaaagcg ttgattcaag caaggttagc
      841 aaacggagga aactatggca agacgaagaa gaaaatcaag agggaaaaaa atatttctga
      901 ttattttagc agttcttatc gtattagtat tagcaattgt aggagtagga gcaaaattat
      961 atctggatct ctcacattct attgagaaaa cttacgaatc agtggaacga acccaaggag
     1021 aacgaacgaa accagttgat ctggacaaac aagaatcttt ctctgttcta ttgatgggga
     1081 ttgatactgg agatttaggt cgggtcgatc aaggaagatc agatacgatg atggttgcca
     1141 cagtcagtcc tgaagacaaa cagacaacga tcgtcagcat tcctcgagat acgtatgtcg
     1201 acatcgtagg tagtgacaca aaagacaaga tcaaccacgc ttacgcattc ggcggagctg
     1261 ccatggcgat ggataccgta caaaaatatg tagacatccc aatcgatcac tatgtctcta
     1321 tcaatatgaa agggctaaaa gaacttgttg atgcagttgg tggtatcgaa gtcaataatg
     1381 acctcacttt ctcacaagat ggctatgact ttacaatcgg taaaattact ttgaacgggg
     1441 atcaggcatt atcttattct cgtatgcgtt atgaagatcc aaacggtgac tatggtagac
     1501 aagagcgtca aagaaagatc gtagaaggtg tagcgaaaaa agtattgagt ctaaacggtg
     1561 tcagcaacta tcaatccgtt ttgaatgctc tagagtcgaa tatgaaaaca gacatgagct
     1621 ttgatgaaat gaaaaaaatt gctttagatt atcgggaggc atttggtacg atcaagcaag
     1681 atcagctgca aggagaaggc ttcatgcaag ataatatctc ctatcaaaaa gtcagtgatc
     1741 aagaacttga tcgtgtgaaa aaagaattaa aagatcaaat gaatctagag aataaatagg
     1801 aaagtggaga aaaaatggaa gagacaatca gcttgaaaga aatttttgaa acattacgca
     1861 aacacttggt aacaatcatc atctcaatgt ttgctggctt ggccatctca ggaatcgtaa
     1921 catttttcat cttgacacca aaatacagtt cacaagcaca attgatcgtg acactgccac
     1981 aatcagacac agcaaatgtc aatgatgtca atacgaacct gcaaatgatc aatacgtata
     2041 aagatatgat cgtcagtgat ttagtactaa atgaagtcaa agatcgttta gaaacagaag
     2101 acaatgtgaa aatgactgct ggacaaatca aagatgcgat ctccgtcaat cagtcacaaa
     2161 attcacaaat gttctcgatc caggcaataa gcacgaatgc agtgactgct caacagatcg
     2221 ctaatacaac ttcacaagtt ttccaagaaa acgcaaaaga cgtgatgaat gtcgataaaa
     2281 tctcgattat ttcaggtgca gttgccaata cgacacctgt ttcgccaaat aataaattga
     2341 atctagtgat tggcttagta ttaggaatga tggtaggcgt aggtttggca ttcttattcg
     2401 aattacttga tcggacagtg aaagatgaaa aatatgtgac ggatacatta ggattcccaa
     2461 tcttaggaac agttccagaa atgtcagcga aagaattgaa cgctggtatc cgtaaaaaaa
     2521 gaacagctcc tgcacgacca aaagctacaa acgataaaac agccccatca cgcagaagcc
     2581 gctcaagagt ctaggaggaa gaaaaatggc acgaacacag aaacaaaaag caacacaaac
     2641 tcatgcagtc agtttgatca cattaacaaa cccatcttca ccaatcgccg aacaatatcg
     2701 tacgatccgg acgaatatcc agtttgcctc cgcagctggt cagcaaatca aaacgattgt
     2761 cgtgacttca tcaggtcctg gagaagggaa atccaccact gcagcaaaca ttgcagtcgt
     2821 ttttgctaaa tccggtcaac gagttctact agtcgatgca gatttacgta aacccgtcgt
     2881 ctataagaca ttccagttga acaatgccag tggattaagt acagcactta gttcatcagg
     2941 aagtgtagca gatgtgatcc aacgaacgcc agtcgagaac ttgtcgattt tgcctagtgg
     3001 acctaagcca cccaacccat cagaattgtt aagctcgcca agaatggatc agatcctagc
     3061 tgaagcaaga cagttatttg acgtggtgat attcgatatg ccaccagtag tagccgtaac
     3121 agatgcacag atcatgtctt caaaaacaga tggcacttta ttggtcgtac gtgaaaatat
     3181 gtcacgtaaa gaatccttga ctaaagcaaa agaactgcta gagatggtac aagcacgagt
     3241 cttaggtgtc gtatacaacg gagcagaaca cagcaaagat gctggttatt actacggaaa
     3301 ttaaggaata agaagagagg agacaagaga aatgatcgat ctacattgcc atatcctacc
     3361 aggaatcgat gacgggtcag aaaacttaga agcaagtatt gcaatggctg aaaaagcgat
     3421 tcagcaaggc attacgcata ttttatgcac accacaccac aataatggaa aatacagcaa
     3481 tgaaaaatca caagtcattt ctctagtcgc ttcgttacaa gccgaattag agaaaagaca
     3541 gttgcctttg acgttattgg aaggacaaga agtacgtatt accggaacgc tcatcgaaga
     3601 catccaccga gatgaaattt tatttacaga tttagatgat acatatcttc tgatcgaatt
     3661 tccgacactg gaagtgcctt tatatgcaga acggcttttt tttgagttgt tagaactagg
     3721 aaagacacca gtgatcgtcc atccagaacg caacgcgcat tttcgagaag acccaaatca
     3781 tttgcttccg ttcctagata tgggatgttt ggcccaactt actgcgccaa gttatgttgg
     3841 tgtatttggc aaagacatcc agaaaacagc caaaacaatg gtcaaacata atctcgtgca
     3901 gatggctgct tctgatgcac acggggtctc taagcgcacc ttttacttaa aagagtgcta
     3961 tgagcagatt gccaaggagt ttggtaagga aaaagtagta cagatgaaac aagtcgcaaa
     4021 ggatctgatc aacggggatg agatcaatta tccaatctat gaagaggtaa aaaagaagaa
     4081 gtttggttta ttttaggggg agttaaaaag tgtttatatt aacaagaaga agaaaaattg
     4141 ccacattggt tgttgtggac agcttgttat taatcgctgc aaatattgca gcagctaaat
     4201 tcatgaagcc gtttgtatct ataccaatgg atcttatttt aatctctatc ggtttgtcga
     4261 ttggttttta tttattttat ggtagtttat tcaaagtttt cactcggatc aatcgctata
     4321 caaatttaag agagatcatt gcaattttta gttcactatc tgcaagtgct gcctcaagta
     4381 tcttcatact gctgtttatt aatcggcgat acagtttacg gttagttatc tttacttatc
     4441 tgctttcgct cttattgatc atcggtagcc gattgatctg gcgtatctac atcgaaaaaa
     4501 aaaaaatatg agatatgtat cagcagattc tgctaaaaat acattgattg ttggtgctgg
     4561 cgaaggcggg cgtattttat acaacagctt tcttggatcc aaaacggctc aagatatcca
     4621 cgttgtcggg tttgtagatg atgatccaaa taaaagaaat acgtatcttt caggaaaaaa
     4681 agtattaggt gcattaaaag atattccgga attgattgaa aaatatgaca ttcaaatggt
     4741 aacgatcgct atcccttcct tatcaagaaa aaaattacga cgaatcttcg agttagtcga
     4801 atccgctcgt gtgaaagtca acacgatgcc ttctatagaa gaacttgctt cagggaaaat
     4861 tagcgtatca aaattaaaaa caattgacgt agtggattta ctgggaagag atgaggtaaa
     4921 gctggatatt gaatccatca aagatcagat cacagacaaa gtcattcttg tcaccggcgc
     4981 aggaggatcg atcggttcag aaatctgtcg gcaaatcatt caattcaatc ctgcaaagct
     5041 attgctttta ggacatgggg agaactcgat ctacttgatt gaccgtgagt tacgcacgca
     5101 tcatcaaaat tgtcctacag aaatcgtacc gatcatcgca gatatccaag atcgcgagaa
     5161 aatcaatgag atcatggaac aatatcatcc agatatcgtc taccatgcag ctgctcataa
     5221 acatgtacca ctgatggaat ataatccaaa agaagcagtc aaaaacaata ttttcggtac
     5281 aaaaaatgta gcagaagcag ctaaagcagc aaaagtgaaa aacttcgtca tggtatcgac
     5341 agataaagct aacaatcctc caaatgtcat gggttcaaca aaacgaatcg ctgaaatgat
     5401 tgtgactgga ttgaatgaag aaggctgtac gaaattctca gctgtcagat ttgggaatgt
     5461 ccttggttct cgcggtagtg tgattccagt attcagagaa caaattgctc aaggtggtcc
     5521 aatcactgta acagactttc gtatgactcg atactttatg acgattcctg aagctagtcg
     5581 tttagttatt caatctggcg cattagcaaa aggtggcgaa atttttattc tagatatgag
     5641 tgaaccagtg aagattgtag acctagcgaa aaacatgatc cgattgagcg ggtatagcga
     5701 agacgaaatc gaaatcattg aaacaggtat ccgtccaggg gaaaaactat atgaagaact
     5761 attgttggat aaggaaagaa atgatgaagc agtttatgag aaaatctttg taggaaacat
     5821 caaaggctac tcaatacaaa aagtgatgga ttttgttaag agcctgccgc aagacgatga
     5881 acaattggct aaagacattg tcacttttgc aaatgcttcg aataagtagg gggagtaaag
     5941 tgaaagcaat tattatagga gctggtggat ttggcaaaga gattgctttt ctattacaaa
     6001 gtgtctctag atatgaattg attggttttg tcgatgattc gttaaaaatg aaaaatcaag
     6061 aattattggg aaaacctgta ttaggaacaa tagattcatt aatagaatta gaagaagaga
     6121 ctgctatttt tctgggaatt gcttcgccag atatcaagga gaaaatatat caaaagataa
     6181 ataaaaataa taaactgatt ttccctaatt tagttgcacc aagtgcacta gtgggcataa
     6241 atgttcagtt agggattggc aatattttga tgccctatac gacttatact gctgacgtag
     6301 ttttaggaaa tttcaatatg atcaatatcg gctctactat cggacatgat gctcaaatcg
     6361 gtaattataa ttcaattttt ccaagtgtaa atatttcagg acatgttata ttaggagata
     6421 aaaatgaaat tggagtaggt actaaaataa tacaaaatct aagaattgga aatatgaata
     6481 ttataggtgc aggaagtgta gtaataaaaa atattaataa taatactaaa aatgttggcg
     6541 tgcctaccaa agtaatagaa agatgggatt aagttggaaa ataaacgaat attattagca
     6601 tctcctcata tgagtgagga aggatatgaa caagagtatg taaaagaagc atttgataca
     6661 aattgggttg ctcctttagg tgcgaacgtg aatggttttg agaaagaatt agcaaaatac
     6721 gtgggagcta gtcatgctgc cgcattgtct tctggaacag cagctataca tttagctttg
     6781 aaagcggctg gggtcggtaa agatgacata gtgatttgtc agtccttgac gttctcagct
     6841 actgctaatc caattctcta tcaaggagct atacctgtat ttgttgatag tgattacgaa
     6901 acttggaata tgaaacctga acatttgcga acagcactag aaaaatatcc caacacaaag
     6961 gctgtattag tagtacattt gtacggttta tcagctaatt tagacgaaat tactaaaata
     7021 tgcaaagaat atgatgttac tctaattgaa gatgctgcag aatcattagg aacaacgtat
     7081 aaaggaaaat atactggtac atttggcgac tatggaattt attcttttaa tggaaataag
     7141 ataatcacaa cttcaggtgg cggaatgctt gtatctaatg acaaagaaaa ggtggaaaaa
     7201 gtgagatttt gggctacaca atctcgtgag aaggcacgcc attatcaaca caatgaaatt
     7261 ggctataatt atcgaatgag caatatcgtt gcaggtatcg gtcgaggaca attgaaagtt
     7321 ttaaatgaaa gaattgagaa aaaacgatat at
//
LOCUS       DAGCYL010000134         7291 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.136, whole genome shotgun sequence.
ACCESSION   DAGCYL010000134 DAGCYL010000000
VERSION     DAGCYL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.136"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..219
                     /locus_tag="MB216_002151"
     CDS             <1..219
                     /locus_tag="MB216_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526947.1"
                     /translation="PKALPDYTGKKAKEDIQSVLKEYERQGILFVSAAIGQDKEEIKS
                     IYGESRFVDITDLNEFPKQMIQLIARYL"
     gene            348..1991
                     /locus_tag="MB216_002152"
     CDS             348..1991
                     /locus_tag="MB216_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBT4526948.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGNGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPEF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(2064..2609)
                     /locus_tag="MB216_002153"
     CDS             complement(2064..2609)
                     /locus_tag="MB216_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBT4526949.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEARLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(2632..3117)
                     /locus_tag="MB216_002154"
     CDS             complement(2632..3117)
                     /locus_tag="MB216_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4526950.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(3107..3919)
                     /locus_tag="MB216_002155"
     CDS             complement(3107..3919)
                     /locus_tag="MB216_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBT4526951.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(4058..5590)
                     /locus_tag="MB216_002156"
     CDS             complement(4058..5590)
                     /locus_tag="MB216_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBT4526952.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRACQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPDLSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEVHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQNAVFLQVYGQSECGPMILKAHTLESLKISD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(5699..6730)
                     /locus_tag="MB216_002157"
     CDS             complement(5699..6730)
                     /locus_tag="MB216_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4526953.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFRTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFELTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVQVSSI"
BASE COUNT         2336 a         1362 c         1351 g         2242 t
ORIGIN      
        1 ccaaaagcgt tgccggatta tacaggcaaa aaagcaaaag aagatatcca gtctgttttg
       61 aaagaatatg agcgtcaagg aatcctattt gtctccgcag ctattggcca agacaaagaa
      121 gaaatcaagt cgatctacgg tgaaagtcgt tttgtagata ttactgactt gaatgaattt
      181 cccaagcaaa tgattcaact gattgcccgc tatctatgat aaaataaagc tcaacgaacg
      241 cacttagcag attcattgag cttttcgttt ctagttaccg gtaacaagga tacttctaaa
      301 atagaatcgg ttataatcaa gagggaccgt taaaggagtg aaacgatatg atacaagaac
      361 aaaaatggtg gaaagaagca gtaggctatc aaatctatcc tgctagtttc aaagatagca
      421 acggcaatgg tcaaggagac atcaatggga tccgtgaaaa acttaattat ttgaaagatc
      481 ttggtatcgg ctttatctgg atcaatccga tctatcaatc tccatttgtc gataatggtt
      541 atgacgtaag tgactatcaa gatatagaag agagattcgg aacgatggaa gattttgatt
      601 tattgctaaa agaagcacat gaattaggga tcaaagtgat tatggatctt gtcatcaatc
      661 atagttcgga tcagcatcaa tggtttgaag aatcgaaaaa atctaaagaa agcccgtatc
      721 gcgattatta catttgggtc gacggggtag atggcaaaga accgaataat tggacatcga
      781 ttttcggcgg ttctgcttgg gagtattctc atgaaactgg acagtattat ctgcatgttt
      841 ttgcaaaaga acaaccagac ttaaattggg aaagcgagaa attgaaagaa gaactattca
      901 atatgatccg atggtggttg gataaaggga tagatggatt tcgtttagat gcgatttctc
      961 atgtaaaaaa agatgaatat tcagtaaaag ccacagagaa ccctttctca ccctttcaaa
     1021 atgtctcagg aatcgaagaa catttgaccg atttgaaaca tgtttttgaa gagtacgata
     1081 tcatgactgt cggagaagca agtggtgtca ctgcagaaga aggaccacaa tgggtaggga
     1141 aagacggtta tttcgatatg atattcgaat ttgatcatat ccatatctgg cagcaagaaa
     1201 aagaagggca attagatgtc cttaaattga aacatgcatt aagcgcttgg caaacttcat
     1261 tggatggtat cggatggaac gctttatata tggaaaacca tgatgttcct cgtgccgttt
     1321 cagtgtttgg agatactcgt cccgagtttt gggctatgtc tgcaaaagca atcgctatga
     1381 tgtatttctt tttgcaaggt acgccattta tttatcaagg acaggaaatc ggaatgacga
     1441 atatgccctt tgaatcgatc gatcaagttg atgcagtaga ttcgaaaaga ctttataaaa
     1501 ggctgcttgc ggaaggtaag acaagagaag aagcattgga tatcattcgt gaaacaacga
     1561 gagacaacag caggacaccc atgcaatgga cttcggaaca atatgctggt ttttctacac
     1621 atgaaccttg gctgatcact aatccaaata ccaaaacgat caatgtagaa caacaagaat
     1681 atgaaccaga gtcggtcctg caattctata agaacatgat ccgcattaga caaaccaata
     1741 aaggtctgat atacggttct tataaagaat atcttcatga acatccacaa ctttatgtct
     1801 atgaaagata tttagaagat gaacaatatc ttattatggt aaacttaaca gaatcacttg
     1861 ctgactatga acttccaaaa gaagcagatc agtcctggac attactcttg tcaaattcct
     1921 ctagcggaga atttgaagct aaaggcatac tagcacctta tgaagctcgg ttatataaaa
     1981 caaacaagta aaacttcctc gttaaagttc attagttgga ctttgcgagg aagtttttca
     2041 gattgctctg tcaattactc atttcaataa ttcgctctag gaccaccgat atatttagga
     2101 cgagagcgaa gggctgtcac gtgagcttgt ccaagggttt tcaaacgagg tctgacaggt
     2161 acttggacat gtttgatatg cataccgatt gatgtgtcac cgatatcgat accggcttgt
     2221 ccaactacgt gttccacttc gacaggatca ttgaactggt caaaagcagc aacagaacac
     2281 gcacctccag catgtagtgc aggaacaaca gagacaattt cgaaattttt tgcttctgcc
     2341 actgttcgtt ctactactaa tgctcggttc aaatgttcac aaccttggac agctagcgca
     2401 atttctttag gatccaacaa ctcttttaac gtacggatga tccattgacc aacttccgca
     2461 ctggaatttt ttccaatatg tcctccaacg acttcgcttg tgctgcatcc taaaacaaaa
     2521 agatcacctt ttttcaagcg tgcctcagcc aatatgtcat taactatttc agtcaattgg
     2581 tcttttaatt ctttttctga caacttcatt ttctaaaacc ttctttttca attaatattg
     2641 cttccgtgct tgaatacgtt gaagacttgc agttatagga aaggcaagtc ccattccaat
     2701 cagattttgg aagatgtttc ctggtataga agccactcca gccggccaat catataaaat
     2761 ccaaccggcg ataccatagc cgatgatcat aactaagctt cctaataaaa gccctaatcc
     2821 ccacatacga gtagaagatt tgctgaaaga accagcaaca tatccttgta acccgtggat
     2881 cactaatgaa aaaactgccc actgcggata tcctgaaatc aaatcgatca atagaccact
     2941 ggaagcacca acaactaatc cacccgttgg accataaaga aaagcagcgg tatagatccc
     3001 ggcctcgcat aatgtcacaa atccattcgt agccggtaca gggatcaaaa ttgttaatga
     3061 aagggcaact gtcaacgcaa tcagcatagc atagatcgtt aattgcttag ttttcatttc
     3121 tcattcctcc tttttgaata ccgccagacc agacgcttcc ggtatgatcg ttcatcagca
     3181 caccattaga aatacaatgg gcaacaaatt ttttactttc tgctactgct ttggtaaagt
     3241 ctgcccctaa cacaagattc gctgttattg cagaagaaaa ggcacaaccg gccccgttta
     3301 ctgttgtagt gtcgattttt ttatgagaaa atacctcata atgatcaatt gaatagagaa
     3361 gatccacagc ttcatctcca gccatccttg tgccgccttt gatcacaatg ttttttgttc
     3421 ctgaagcagt gagttcttta gcgagatgga tcatttctgt taacgaagag ggaagagctt
     3481 gaccagaaag taaagcagct tctgccaaat taggcgtaat catcgtaaca agtggaaaaa
     3541 gttcttcgat gagctgcgaa atatacaatt gattgatcac agcttcagtt tctttgaatg
     3601 ccaaaacagg gtctaaaatg attgggatat tttgttttgt ttttatgaag tctcgaacaa
     3661 ccatgattgc ttcacttgag ttcaataacc caattttgat accatctaat gaagtcattt
     3721 tatcaatcga aactaactgt cgtttaagca attccaaaga tatagattcc agcataaatc
     3781 gctcattaat atctagtgcc cctatagaag tcaaggcgct tagtccgaat agaccaaaat
     3841 tctcaaaagt ttttaaatct gtttggattc ctccaccagc aaatggatca gaaccgccaa
     3901 ttgtcaagat tcttttcata acattttcct ccatgaataa gagtataacg aaattttcaa
     3961 aaaagaaaaa cagccaattg gcatattttt atctcatcca atttaaaaaa acaaaaagaa
     4021 aagaaagacc aaaatggatc tttctttcct taaatagtta ggacttttgt gattttaatt
     4081 ctttctcgat ttgaagccgt tgcactttca ttgtagctgt tcgcgggatt gcatcaaaat
     4141 cacggatgat agggacgttc aagcgaggaa gttcatggac ttgctcccac catcgattcc
     4201 aatccatttc tttgtcagga gctaaagcaa taatcggttg tggagaatga tttttatccc
     4261 ttacaatcac tacttctgcc aaaaaatcta acgaatctaa taaaaagtct tcaatagcta
     4321 atgtactatt gatcgtttcg atcaaatcca cttgccgatc tttcaaaaat aggtggccat
     4381 gcttatccat aaaaccgtaa tctccgctat cccaccaatc gccataaaca ttttcctgga
     4441 accgtgcatc ttccttgtaa taagtcaaag cccgtccttt agacagtaat tgtatatgac
     4501 catctgtgtt ttcaggtagg acattcccaa ctgaatctgt aatccgagcg cttgtcatgt
     4561 cttccagtcc aacgcccata tctcttgcat cacttatttt caaactctct agtgtatgag
     4621 ccttaagaat cattggtccg cattcacttt ggccatatac ttgaagaaat accgcattct
     4681 gtgccatcga cgttcgcaaa aatgtcttca ttgtctggtt gtttatagca tcaaaagtgg
     4741 agtgatagta tcggataccc gaaaaagctt ctggatgctc cttagcagta aaacgccact
     4801 gaacaaagtt attcggatgg gtctctagtg caattggccg gtgaacttcc aacatatgaa
     4861 caacttttga actttgtgcg tttgctaaag gcatcatagg aaatcccatg gacatcaaag
     4921 aagagatacc aatattgaat ctggaatgca ctggagagat gtgaaaagcg attaatttct
     4981 tttccgaaat ttttgtgaaa actgtttttt gccatttcgt ccgccatccc atagaatgag
     5041 cagaatgaca aataagttta gggataccag ttgtaccaga agtatgggtc atataagaaa
     5101 tttcattttt gtctaaaaat gattgagaaa cagacaaatc aggttgttcc attaaataaa
     5161 gaataggaat ctgttttgat tttgttccat ttgaaatttc tctcactcgc ttttttgtct
     5221 catcatcaaa taaaatgaaa gggtcttcta aacgatcgat aaatacttcc attgttgttg
     5281 ttggtagatg ataagaagtc atgacaggca ctgcaccaag gtaagacaca gaaacagcta
     5341 gaaggtatgt gtcaaaagcg gaactcttat aaataataac tttgtctccg gatttcactc
     5401 caagatgagc caattgacaa gcccgtttca acacactttc gcagcatttt ttgtaagttg
     5461 tttcaagtcc taattctggg aaagctgaaa gtggctcatc aaagataatc ggggtctcgg
     5521 aaaagttagc tgcagcttct tgaaaattcg taaataagtt caaaggggaa tacgttatgg
     5581 tctccatcat tcacactcca ttatcttttg ttaagtaata cacaaattca agataatcgt
     5641 aacataatcc tttatatagt aacatagttt attgtgaaaa aaagatcata catttgtttt
     5701 atatggaaga aacctgaacg aatgccgcta attcgtgata agaaaaatca accatctttt
     5761 cccagatatc ttgaaaagaa tgggatgaga ggtatcgatt accacataaa taatttaccg
     5821 cgtcagcatc atctaaatat agttctgcat aataacgata tgtttttctg catagttctg
     5881 gcgatacgtt tttatatact gtaattttgc tcaaaatttg gcgaatagtc cgaggagaaa
     5941 tttgctgatc atgtgaggaa cgaaaaacaa aagaattttc gtccgtattt tgtgctgtta
     6001 actcaaaata gttctccaga aacggcatcg cttcttttgg tattggtact ttattcagtc
     6061 tgttttcttt tctattcaga ttaagaatct gcttggtcaa agatacatct ttacgtttaa
     6121 gttctactac ttcatggatc tttaatccgc aatacagaag aatggcagta attgcaaggt
     6181 tcctcggtcg attcttttct tcaaagctag cttctctgct tgttcgaaag ccttcaatcg
     6241 ggataaacaa atgctcaaaa tctttgaaaa gttctgcatc gatatatcgc ggagctgctt
     6301 tggttgtttc cggtaaactt gtacccaaaa gagaaatctc ttcttcaatc agtccttttt
     6361 gatacaaaaa tcgtaaaaaa tgatttaatg aatattgctt ccttgaaatc ccgtaatagc
     6421 tattttcaaa caattgctcc gtttcttggt tcgctgtagt ttggaacgaa cgagtatgtc
     6481 gatagatttt agctaagaaa ttctgataat cttgtttttc taaatggata atcagctgat
     6541 catccacttc ttcagaaaat acttttctca aataatcagt aaacagatga aaatcttttg
     6601 cgtattcata gcgactgcct aatgaaaggg aagaacgatt ttcttctaaa aactgttgaa
     6661 agacggaagg gagcaagtgt gtacgttgct ttaacttttc ttgcagttgt tttctagttt
     6721 tgctgtccat tacaaattcc ctccaatagt gttcattttc tatattttac cgcatttcat
     6781 caaatgaaga tagacagttt gtcttgatct taggaagcaa atttttaact gtcttttaaa
     6841 agtactagct aaaagatttg aagtaagaag atacattttt agttatttta ttagtgcagt
     6901 cattcttttg atattttaat tgatttatcc tctttttagc tatgtggtta cttcatgagc
     6961 aatgaaatgg atttggttcg ttctaatggc aggaggcttt ttcttttatt tccgaaaaat
     7021 tagaaaacat tttttcagaa tcgattggct ctccatttat tcaataatag tttgtctaac
     7081 gatattgttg ggataattct taaataagta ttagaaatag ttggtacttc attactttac
     7141 atgaagattt attctatggt aggagcaatt atactgagaa ttaatccttt tttatataag
     7201 ttgtttgctt attgatggat tggataaatt aaaccatcac aacgagtgga aattatatga
     7261 aatttttcat aaaaattcat atagaaacaa a
//
LOCUS       DAGCYL010000135         7269 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.137, whole genome shotgun sequence.
ACCESSION   DAGCYL010000135 DAGCYL010000000
VERSION     DAGCYL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.137"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            288..557
                     /gene="rpsN"
                     /locus_tag="MB216_002158"
     CDS             288..557
                     /gene="rpsN"
                     /locus_tag="MB216_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBT4526954.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            572..751
                     /gene="rpmF"
                     /locus_tag="MB216_002159"
     CDS             572..751
                     /gene="rpmF"
                     /locus_tag="MB216_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBT4526955.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            783..1160
                     /locus_tag="MB216_002160"
     CDS             783..1160
                     /locus_tag="MB216_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526956.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1181..1330
                     /gene="rpmG"
                     /locus_tag="MB216_002161"
     CDS             1181..1330
                     /gene="rpmG"
                     /locus_tag="MB216_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBT4526957.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1354..2337
                     /locus_tag="MB216_002162"
     CDS             1354..2337
                     /locus_tag="MB216_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBT4526958.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2367..2489
                     /locus_tag="MB216_002163"
     CDS             2367..2489
                     /locus_tag="MB216_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBT4526959.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2507..4030
                     /locus_tag="MB216_002164"
     CDS             2507..4030
                     /locus_tag="MB216_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBT4526960.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4054..4293
                     /locus_tag="MB216_002165"
     CDS             4054..4293
                     /locus_tag="MB216_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4526961.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4486..5121)
                     /locus_tag="MB216_002166"
     CDS             complement(4486..5121)
                     /locus_tag="MB216_002166"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBT4526962.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5142..5360)
                     /locus_tag="MB216_002167"
     CDS             complement(5142..5360)
                     /locus_tag="MB216_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4526963.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5486..6690
                     /locus_tag="MB216_002168"
                     /pseudo
     CDS             5486..6690
                     /locus_tag="MB216_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180763.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            6685..7092
                     /locus_tag="MB216_002169"
     CDS             6685..7092
                     /locus_tag="MB216_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBT4526964.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
BASE COUNT         2564 a         1083 c         1429 g         2193 t
ORIGIN      
        1 tgccgtttta agtagaggaa aagctactta aaacggtatt tgtgtttgat tggactaata
       61 aaagtgaatt cttatttttc agagcttaaa ctgcatattc ttttaaatga tttgattatc
      121 tctatgtacc gataaattcg ttttcaaata gttatctatt catatgagtt ttatttcatg
      181 ttatttattg ttgacaagag caactcttgt tggtaaagta tcagtgtcaa aacgtaacga
      241 ttacgatttg agatgtctaa atcaaaataa aaggagtata aaggtatatg gcaaaaaagt
      301 cgaaaattgc taaagcaaag aaacaaatgg cgatgattga aaagtacgca gataaacgtc
      361 aggaattaaa agccgcagga gatcgcaccg ctctcgctaa attgccaaga gattcaaatc
      421 cgaatcgtct gaggcttcgt gatcagacgg atggtcgtcc aagagggtat atgcgaaagt
      481 tcggtatgtc ccgtattaag tttagagaac ttgcccatca agggttgatt cctggagtaa
      541 aaaaagcaag ttggtaaaag gaggaaatat catggcagta ccagcaagaa aaacatctaa
      601 ggcgaagaaa cggttaagaa gaactcatca aactgtagta aaaccagaaa tttcatttga
      661 cgaagcgaat ggtgattata gacgtagcca tcatgtatct ttaaaagggt actataatgg
      721 aaagaaagta attaaaggag cttcaaaata gtttaatata taacttttag gagggaatca
      781 aaatggaaat tatttaccca cctttagttg aagaaggatt aaagtattat cttgaaacga
      841 cccagcaatc gctagataaa tgcacttttt atcgttcgat ggtagagaga ggaattatta
      901 ctgaaacggg tttaccgacc caacaggcaa ttgaaaatgg attagtcaaa gattactatg
      961 aggaccaagg cttatcattt gatgattttt taagaatcta tccaattttt gaagagtatg
     1021 acgaggagtt atttcaatgt attgatggtt attgggagat acctgttgat atgaaagaaa
     1081 atttagtttc acaattggaa tctggcgagt taacttttga agatgcacaa caaattcaag
     1141 catatcttga agatcgataa gtattgaagg aggaagattt atgcgtcaaa atattatttt
     1201 ggaatgtgtc gaaactggag aacgtctgta tctcaccagc aaaaataaac ggaataaccc
     1261 tgaacggtta gaattaaaaa agtattcgcc aaaattgcgc agacgggcaa ttttcaaaga
     1321 agtgaaatag ctaagtggta aaaaggtgac aaaatgggca taccagttac aatcgtttct
     1381 gggtttttag gggctggaaa aacaacgcta atcaatcaag ccttgcaagt ctcaccctac
     1441 cccagggaag aaattattat aattgaaaat gagtttggtg aagttgggat tgatcacaaa
     1501 ttattagttc atagcgagga acgggtgtta caactaaata atggatgtat gtgttgcagt
     1561 cttcgcagtg atttattagc aatattgatt tctttactgg aaacaataga agaacatcaa
     1621 caaccaattt cacaaatcat cattgaaact acaggtatag cagatcccca accaatcatt
     1681 caaacacttt taacagcgcc tcaactaaga ggaagagtat atatcgatag tttattgact
     1741 gttattgata gtgacagttt tgaacttatt gaaagagaac cacaagccct taagcaactt
     1801 gtcatagcag atcgcatttt tgtgtctaga aaacaaaact tgaatttatc tgcactaaaa
     1861 aaattacaga gagaaatcaa aagtattaac cctttatccg atattcgaat gttcacacat
     1921 agtgaaacga ttcaaaaaaa ggatttttat aatttggaaa aattcaatca tcccttagtt
     1981 tttgaaagtg attcatcaga aaatcactat aatggcaaac actatcatca acatcatcaa
     2041 gaacatgagt ttaaatcaat tctcttaact tcagatcaag acataaaaaa agatttttta
     2101 ttccaattaa ttgaccggct gattttaacc aaccaaggga gcctgtatcg ttttaaagga
     2161 ttgattgctt tgcaagatca agattttata gttgccatac aaggggtaaa cgaacaggtt
     2221 aattttcaat acaccactcg acaaaaagac caaaccacta ttattctaat tggaaaaatg
     2281 ttggatgaag aaaggatcaa acagagtttt gataaattac tggcggaaag caaataatga
     2341 taagtattga ttaggggtaa tttctaatgg agaaaacgga tatatctagt gcatatcgac
     2401 gacttaaaag cccgaatatc aaaactagaa aaagggcatt aaaaattatc aaagaacata
     2461 aacaaaataa aatgaagaaa ttagcataat taaggaagga aaaagaatga agaaaatcag
     2521 tgtaggactt attgggatag cagctctagg attattggga gcttgctcca gcactaatga
     2581 tgcaaaagta tcgaatgata aagatggaaa attagaaatt gtaacaacat tctatccaat
     2641 gtatgatttc accaaaaaca tcgtcggcga tgaagcgaac gtggatttaa tggttccagc
     2701 gggatcagaa cctcatgact atgagccctc agctaaggat atggcaaaag ctcatgatgc
     2761 tgatgttttt gtatatcata acgaaaacat ggaatcatgg gtaccaaaag ctaaagaaag
     2821 ttggaagaaa gctgggccta atgtagtaga aggaacaaaa gacatgattt tattgccagg
     2881 tagtgaagaa gaagatcatg atcacggaga agaagatcac catcatgaac tagatcctca
     2941 tacgtgggtt tcacccaaaa tggcaattaa ggaagtttca aatatcaagg atcagttagt
     3001 taaattgtat ccgaaaaaag caaaagtatt tgagacaaat gctgaaaagt atttgactaa
     3061 actgaaacgg ttggatgctg actataccac tagtcttaaa gaagcgaaac aaaaaagttt
     3121 tgtgacacaa catgcagcct ttggatattt agctttggat tatggattga tacaagttcc
     3181 tattgctgga ctaagtcctg aagaagagcc atcttctggt cgattagctg aattaaaaga
     3241 gtatgttaaa aaaaataaga tcaattatat ttactttgaa aagaatgcta atgataaaat
     3301 cgcaaaaact ttggccaatg aagctgggat aaaattggaa gtattgaatc ctcttgaaag
     3361 tcttacaaaa gaacaaatgg ataatggtga agattatgtt tcagtaatgg aagacaatct
     3421 aaaagcgtta gagaaaacaa ctatggttgc tggtgaagag gtagttccag aaaaagaagc
     3481 gaaggatgaa aagacagttg ctaatgggta ctttaaagat gccgatgtga aggatcgcga
     3541 attgagtgat tatacaggcg aatggcaatc tgtttatccg ttgttaaaag atggaatttt
     3601 ggatgaagtt ttcgattaca aagcaaaact caataaagac atgacagctg ccgagtacaa
     3661 agattattat actactggct ataagacaga tattgatact atcaacatta aagacaacac
     3721 cattgatttt gtagtcaacg gtgaacatca tcaatacaca tacaaatatg ttggatacaa
     3781 aattttgaat tatgaaaaag gaaaccgtgg tgttcgtttt aattttgaaa ctgatgatgc
     3841 aggtgctggt cgttttaaat atattcaatt tagcgatcat ggaattgcac caagtaaggc
     3901 agagcatttc catattttct ttggtggaga aagtcaagaa aaactttata atgaaatgca
     3961 taattggcct actttctatc cagcttcatt atctgagcat gagattgctc aagaaatgat
     4021 ggcccattaa aatccaatct ttggaggaaa tacatggtaa gaaaagtatt attgtctatg
     4081 gattccaaga atattgatgc ttatcggcgt tatcaatttg caaagcaaat gagattaaat
     4141 gggagtacat tagctgatat tgctgcaact ttacatgtta gtattgacac agttcataga
     4201 gtattaaaac gggatcctga ttgttgtttt atcaaggtca atactggaag aagtagacgt
     4261 ttagcaaaac ggcataatag cttggccaat taattgataa taccagagta gatcattttt
     4321 cccttatttg gatttgatct gctttttatt atttcttgaa ttgtcaaatc aagcgcaaca
     4381 ctttatcaaa gaagacttca taaggtgttc tccaatctaa acactttcta ggtctgttat
     4441 tcagtttttc aatcatctga tttatcgttg agttatctac cgtagctatc tctttcgatt
     4501 ttggcaagta ttctcgaatg agtctattcg tattttaatt tgttcattgt tgccaagggc
     4561 atgaggatct ggaaaataaa agggcatatt attcaatact tctgtaactg cgaaatgatt
     4621 ggagaattct tttcctctat ctggagtgat cgttttgacc gcctcatttg gaatactgct
     4681 taaaagttca atcattttct cgctcacata agcggctttc tttctgggga ccttctttcc
     4741 tgctaaaagg aagcatgatt ttctgtctgt caaagtcact aaacaagtac cactagtttt
     4801 gccagctaca atatcggctt tccaatgacc aaaatcactt ctatcatctg cggattttgg
     4861 atggccatgg atcgaatgag taaggcaaat cttcccacga gactcgacat gattttttgt
     4921 atgtcttgtt tttcctcgat ggtgcaatga acggatcagg cctttattgc cgtgtgagag
     4981 gggttctgta tcgaaaagat cattgcaaat tgcacggtag atcgtgataa aactaattga
     5041 aaagtccata tgttcatgct tcaaacgatt cgagattttc tctggcgacc actgaaaatc
     5101 gacaaataaa tgacgaacaa ttgaccaaac tcacggatca tttagaatgt gataacgtcc
     5161 gcattttttt cttcgataag cataattttt ttccgctttg gaaggtgatt agtttttttc
     5221 ttgtttcttc tcagttcatg taaaatagta aacgggctat gctgaatgtc gttgccaatt
     5281 atactaatag aaaatccttc ttcgtgaagc agaaagattc tttctcgttt gagtatagta
     5341 agatgacaat actggctcat aattattttc cttctaggag tgttctcgac aaacttatac
     5401 tagttggata tcgattatga ctcattttta tgtgttgcac ttaaagtgta aattcaaggt
     5461 atagaaagag gttccctatg aaaaaatacc tattacaatc ttaactggct atcttggttc
     5521 aggaaaaacg atacttctaa atcatattct gtacggtgat cacggaaagg aaattgctgt
     5581 cattgtaaat gacatagggg aagtaaatgt tgatggggta ttagtcgaca agaaaggttt
     5641 taaaagaact ggagaaaaac ttgtttcgat gccgaatgga tgcatttgtt gtacattacg
     5701 cgaagatttg attgttgaac tagatcaatt agtgcaattt tctgacttga atcaaattgt
     5761 tattgaagct tcaggaatca gcaaaccaat tccaattatt caagcaatta tattagctaa
     5821 aaacccttta gacattgatt tgacccaacg attagagatt ggtgccatta ttatagtagt
     5881 ggatgtaaaa cgtttttggg ataatttcga aacgggtgaa aagattcagg aaagaaaagc
     5941 tgaaggtaat gtagttgaag aagaacagga tattcgtggc ttgttaattg atcaattcga
     6001 gtgttgtaac atattacttt taaataatag tgacttagtg tcaaccaaga cgatttacaa
     6061 aattaacggt ctaattgcta agttacagtc caatgccaaa ataattagga caattaaagg
     6121 tgaagtagac ttatcgaaaa ttctaaatac aaatcttttt aatatagatg agacttccta
     6181 ttcaatgggc tggttcaaag agttagagct tggctatcaa aatcatattc ctgaaacaga
     6241 agaatatggt atctcaagtt ttatctatcg aaatcggtta ctccttgacg cacaaaaatt
     6301 tgcccatctt acggataaaa attttcctgg caatattaca agagcaaaag ggtttctttg
     6361 gtttgcccaa catagtgatt tttcgacgat ctttgaaaca tcgggtagcc ataaaagtat
     6421 tgaatcatta aattattggg tagctagttt acctaaaatt gagtgtcaaa tgattataca
     6481 aaaaaacccg aattgaagga agcctggcat ccgttgtttg gtgaacgcat taataaacta
     6541 gtttttattg gtatgaagat ggatcaacaa tgtattaagg aacaacttga gggttgttta
     6601 actgattctc ttaatgcaga taatttccga aaaatttcac catttccatg gaaaaattct
     6661 aatatgttta gccagatatc gttaatgaaa tttttaagaa aagctggtgt caagataggc
     6721 gatgtcataa ttattggcat tttgattatt ccctctttta ttcccttatt tgtattctct
     6781 atagaaaagg agaatgaaac aactgaagat gctatcaaat atgccatcgt tcaaattgat
     6841 ggcaaggaaa tggatcgctt cgatttggat aagatcaatc ataaactcgt gaattatcat
     6901 cctgcgaagg gacaatataa tattgttgag attaaagatg gaagaattcg agttaaagaa
     6961 gataatagtc ctgatcaaat cgcggttaga actggatgga tctcaaaacc cggtcaaacg
     7021 agtatttgtt taccccataa gttagttatt agtattgaga aaaaagaaag caaagattac
     7081 tacatttact aatctggaaa tagatgagga ggtttttaat aagaaaaaga tattattatt
     7141 aataatagca ggggctttat taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt
     7201 gacaacaggc acaagtatct ttaacgggac ctgcaatggt ttagttaaca gtcggttaga
     7261 ttacttatt
//
LOCUS       DAGCYL010000136         7247 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.138, whole genome shotgun sequence.
ACCESSION   DAGCYL010000136 DAGCYL010000000
VERSION     DAGCYL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.138"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="MB216_002170"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="MB216_002170"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HBT4526965.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="MB216_002171"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="MB216_002171"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HBT4526966.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="MB216_002172"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="MB216_002172"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HBT4526967.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="MB216_002173"
     CDS             complement(4028..5044)
                     /locus_tag="MB216_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4526968.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5343)
                     /locus_tag="MB216_002174"
     CDS             complement(5146..5343)
                     /locus_tag="MB216_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526969.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="MB216_002175"
     CDS             complement(5315..6634)
                     /locus_tag="MB216_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4526970.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWQERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
BASE COUNT         1910 a         1748 c         1237 g         2352 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac attctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttgccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcgttt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaact ttttatactt
     6721 tctttactgt ctaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
     6781 ttcaaatttg tcttcgccaa cacaatttga cgaagagcct tttctttcag gaagacttcg
     6841 gtaattttta ggagttctac ctattctttt aaccgtttat tctctttttc taagcgtgta
     6901 atttcatttc tgcgaatgga tttcatgact tcctctaccg agaaaagtac gttatccata
     6961 tttttcaact tcttgaactc atcaatacac gctattagag cgagcttcta gctatagact
     7021 aacttcttta acagaaaacc cttcttccgt taccagtttt acagcggtaa ttttaaatat
     7081 ttcatcaaat gaacgtcttg gcataatcca gatcttcgct ttctcttaat ttggtgtcaa
     7141 ttttagtata acccgtccaa cttatttttt aactaagtgc ccggtttttc ttgacaactc
     7201 cacccaaaag ttagattttt tggtctaagt tttagggtcg cttcatt
//
LOCUS       DAGCYL010000137         7220 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.139, whole genome shotgun sequence.
ACCESSION   DAGCYL010000137 DAGCYL010000000
VERSION     DAGCYL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.139"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(145..624)
                     /gene="rlmH"
                     /locus_tag="MB216_002176"
     CDS             complement(145..624)
                     /gene="rlmH"
                     /locus_tag="MB216_002176"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBT4526971.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1052..2341
                     /locus_tag="MB216_002177"
     CDS             1052..2341
                     /locus_tag="MB216_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBT4526972.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2380..2985)
                     /locus_tag="MB216_002178"
     CDS             complement(2380..2985)
                     /locus_tag="MB216_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBT4526973.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(3181..3645)
                     /locus_tag="MB216_002179"
     CDS             complement(3181..3645)
                     /locus_tag="MB216_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBT4526974.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(3667..3987)
                     /locus_tag="MB216_002180"
     CDS             complement(3667..3987)
                     /locus_tag="MB216_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBT4526975.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(4130..5209)
                     /gene="pepA"
                     /locus_tag="MB216_002181"
     CDS             complement(4130..5209)
                     /gene="pepA"
                     /locus_tag="MB216_002181"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBT4526976.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5409..5759
                     /locus_tag="MB216_002182"
     CDS             5409..5759
                     /locus_tag="MB216_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBT4526977.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            5944..6897
                     /locus_tag="MB216_002183"
     CDS             5944..6897
                     /locus_tag="MB216_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4526978.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            6910..>7220
                     /locus_tag="MB216_002184"
     CDS             6910..>7220
                     /locus_tag="MB216_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526979.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     I"
BASE COUNT         2138 a         1381 c         1364 g         2337 t
ORIGIN      
        1 aaggataaat cgtcgtatca aagctcattc ataagtagta aattagtagt aaattgagtg
       61 gttttgacct tgataaagtg tgataagtcc agtttttatg cggataacta gatttttatg
      121 ctatttttct cttaaaaaat atcattattt gtggtatgga gcccccgcgt tgatccggaa
      181 ggcacggtag atttgctcga cgaggatcag gcgcatcaat tgatgtggat acgtcatttt
      241 gccaaaagag atctgagcat tgcttctttt catgacggct tcgctgagtc ctaaagaacc
      301 gccgataaca aaaacaaggt ggctttttcc ttggatacct aattggtcga tttgtttagc
      361 aaaagattcg cttgtgggat ttttcccttc aattgctaag ccatagacgt attcttgttc
      421 tttgattttc gctaagatcc gttctccttc tttttctttt acttgtctca tttgcgcttc
      481 gcttaaattc tccggggctt tttcgtcagg aacttcgatc aattcgattt tcgcataggc
      541 attcagccgt ttgacatact cattgatacc ttggatcaag tatttttctt ttaattttcc
      601 tactgaaata attttgatat tcatgttttc tctcctttga tcttcacgct cactttgttt
      661 attataatca attttttcgt ctatatgttt attttgacta tttttgttaa aaagatttcc
      721 ccaacagtta tgcacaacat ccacaggttt atccacagaa aacagcgttg ttttacctta
      781 tttcatcaca ttcattaggc aaacatacgt tttataagaa tttatccact ttatacacag
      841 gctgtggata tgttttatcc cttatttttc caatcctatc cgcgtagttt tacacaatat
      901 ctgtggaaaa agaaaatcaa atattttcat ctgaaaacaa tgatttttcc taaagttttt
      961 cataaaaagt tcaaagtctc aaagtagact aaagtcaaca aataagacta tactaacagt
     1021 agataaatga atgtaattgg aggaggaaca catggataga aaaaatgtga caccaaagat
     1081 gaaaaaaaat aaaaataata gcctctggcg taagttgggt ctggggcttg ttggagggat
     1141 cgttggagga ctactgacag ctggcatttt ttatgcagcg atgggcactg gcaacaatag
     1201 ttctactgct acaagcggca atcaaaattc tgccggtgaa acagttgtgg aaaatgtcaa
     1261 agtcaatgta gattctgata tcaccaaagc ggtagataaa gttcaagacg ctgtcgtttc
     1321 tgttatcaac ttacaaagtc aaaaccaatc aagcggtttt ggcggattat tcgggcagca
     1381 agaagaaagt agcagctcag atgatagtaa tttggaagct tatagtgaag gaagcggcgt
     1441 aatctacaaa aaagacggga atactgctta tgtagtcaca aacaatcacg tagtagatgg
     1501 acagcaaggt ttggaagtca tgatgaaaga cggtacgaag gtaaaagctg aattagttgg
     1561 aacagatgcc tattcagatt tagcggtgtt gaagatcaat tcagacaaag tcgaaacggt
     1621 tgcctctttt ggggactcaa gtgctttgaa ggttggtgaa cctgcgattg cgattggttc
     1681 cccattagga tctgaatatg cgaattctgt cacttcagga atcatttctt ccttgaaccg
     1741 acaagtaaca agtacgaatg aatcgaacga aacagtcaat atcaatgcga tccaaacaga
     1801 tgcagcgatc aacccgggaa attctggtgg tcctttagtg aatatcgaag gacaagttat
     1861 cggtatcaac tcaagtaaga tcgcaagtac ttctgaatcg acttctaatg taagcgtaga
     1921 aggaatggga tttgccatcc caagtaacga tgtagtgaat atcatcaacc aactggaaaa
     1981 agacggaaaa gtgacacgtc cagctctagg tatcacaatg gtcgacttat ctgccgtttc
     2041 tactcagcaa caagaacaaa tcttgaagat tccttcatct gtaactaacg gtgtcatcgt
     2101 gacaaatgtc caaacagcta caccggcaga aaaagctggg ttgaaacaat acgacgtcat
     2161 tactaagatc gatgacaaag aagtttcttc tggtgtagaa cttcaatctg ctttgtataa
     2221 gaaaaaagtg ggagattctg tcaaaatcac ttactatgaa ggctcgaaag aaaaaacaac
     2281 tactgttgaa ttgacgatcg atcaatcggc attaaaacaa aatcaaaata gtgaaaatta
     2341 ataaaaaaga ggttgtgaca aagacttgtc acaacctctt tattttggaa atacttctcc
     2401 gactacggca tcctcaggca atactaagat tccttttttt tctggagcat ttggcaaatg
     2461 caattcacga gcagaacaaa tcattccata gctggctacc cctcgcaaag ttcctggcca
     2521 gatcatcatt ccatctggca tcatggctcc tggttttgca acgacaactt tttgtccagc
     2581 ttcgatattt ggtgcaccgc aaacaatttg caatactgtc ccattgtcca cttctgtctc
     2641 agtaatcgat aaatggtcag agtcaggatg cgggacgcat gtttttacat acccgacaac
     2701 gatcttcggt tcatgatcag cttcaagctg ttcttcgaat cctgcttctt taatcaatcg
     2761 attcaatttc gctaattctt catctgttaa gcttacttgg ccgttccctt caattgttag
     2821 atgatcggag atatggaaaa agttccaagc aacaacttga ccttgttcat ctttgatccg
     2881 agcaacattc catttacgtt cgacagtgtt ttctgttcct ttatcgtcgg ctatgataac
     2941 catcaaagtg tctccgacgt gtgctttgtt ataagcaaaa atcatgttct ttcctccatt
     3001 atcctttgct gagatttatc ttaacaaaaa attgcattca ctgcatgaga tttttttcat
     3061 gctttatttc cgaagaagct tcttctggaa caccatttat tcgtaacaag ggactgtgac
     3121 aagacttttg tcacagtccc tttactgttt gattaaaaag gttatacaag ttccacttgc
     3181 ttaattactc ggctgttctt cttcgtcaga aacgatcaag acatcacaag gtgaatgacg
     3241 tatgacgtag ctggctacac ttcctgtcat aaagcgttcg actgcattta gtccagactg
     3301 cccaaccatg attagatcga ttgcatattt ttttggcaat ttgactgcca tggattcacg
     3361 aacagagccg tatgcaataa ttccttctat atttgtgtaa tcaacagatt ttccatattc
     3421 tttacattct tcgatcattt gcttggctgt ttcagtttgc tgatcgacta gattttgatc
     3481 aataggagca tagcccataa aattataaaa ttgctgatcg attacgtttg ctacatatac
     3541 tttcccatta ttacggcgag ccacttctaa cgcttttttg aaagcagcaa gtgcttgttc
     3601 actaccgtca atacctacta aaatgttctt atattcttga ttcatcttga cccctcctaa
     3661 cagatgtcag ccttttatta cactttctag gaacgattcg atttcctgtt ttgttttacg
     3721 atctttattc actagacgac ctaattcttt tccgtcttgg atcacgacaa aacttgggat
     3781 accaaaaatg ttccattcag cggctacatc tatgtacttg tcacggtcta cttcaataaa
     3841 ttgccaatca gaaaagtcat tttcgatttc aggcatttgc ggtttgataa aacggcagtc
     3901 tccgcaccag ccggcagtaa agaaaaatac actttttcca gtttctacgt agcctgctaa
     3961 ttcttctaaa ctatttggga taatcattct ttttcctcta ctttctgttt atgtattttt
     4021 caattaaatt atacaccttt ctttcgaact aacaaacatt gatcctcttt ttataaaaaa
     4081 aatctgacaa aaggttgctt ttgccagatt ttatagtttc actatcgcgt tatttcccgt
     4141 atacgatcgt gttcactgtt gatttatcta aacctttcaa tgtttgaatc aacatttctc
     4201 gtgcagcttc aaaatcgcga atactgaaca tcgtttgatg cgtatggata taacgtccca
     4261 ctacgccaat cactgtactt gggattcctt cattttgtgt atgagcagca cctgcatctg
     4321 taccgccttt tgaaacaaag tattgataag ggatgttgtt tgtttcagcg atatctagca
     4381 agtactcacg caaacgagga agcatgatca aacctggatc ttggatacgc atcaatgtac
     4441 cttctcctaa atgtccgaat gtgccatttt tcgtcactgt gtcatctgct gcagagcagt
     4501 ctactgcaaa gaacagatct ggcttgaatt tattgacaga agctttagaa ccacgcaagc
     4561 ctacttcttc ttggacattc gcgcctgcaa tcaatgtatg acctaatgtt tcattttgta
     4621 acgcttctaa agcttctaat accattgtac agccatagcg gttgtcccac gatttgctga
     4681 tgatattctt cccgttggct gttttgatcg tttcagcgta aggaacgatt gtgtctcctg
     4741 gaagaacacc gtaactcatt gcctcttctc ttgattcaaa cccagcatcg aacaaaatat
     4801 ctgttacttc tactgatttt tgtccgcttg ttcctcgtaa taaatgtggg ggaacggaag
     4861 aagaaataca tggataattc ttaccagtag aggttttaag tgtaaaacgt tgagcagaaa
     4921 caacgtaagg gttccatccg cctagaggta cgactttaaa aagcccgttt tcttggattt
     4981 gtgtcagcat gaatccgact tcatccatat gtgctgcgac cattacacga ggtgcatccg
     5041 cttcttcatg gtggcgcagc ccaaaaatgc cgcccaaacc atctaactgt aactcatcta
     5101 ccagcggttc catatgactt tccatatagg cgcgaacgtc ttgttcaaaa ccgcttgttc
     5161 cttgtaattc tgttaactct ttgattcgtt gaaatgtttt ttcttccata tgctattcgt
     5221 ctaaatttca gactaaactc ctctcagact tatattgatc tgcggataaa atatgatttt
     5281 ttctgcagac cgatcgtatt tattatagct tattttttct ggtagcggtt atttatttca
     5341 aagaaaatat gagagtgtgg taaaataaac acaaagagta gcaagtgaga agaaggaggt
     5401 atatccacat gtatgaagaa agcgaattaa gctattttaa aggcgggtta gccgttggga
     5461 tcggtctagg attattgagt ggggtagctt caacgatttg gtatcatcgc aaaaaagtgt
     5521 tatcagcaga ccaagtgctt gaaaatatca aaacatcttt tttaaaagaa ggcgcaatcg
     5581 aaggtgcttg gatctctttt gaaaaagaac ctattcggaa attcgccgtt cggtcgagtg
     5641 cttatcgggg tgggctttcc cggatagaag atggcgaaac tgtttattat gaatttttag
     5701 cagatgctca tacaggaacc gtattagaca tttcaagaaa aaagggcgca atagattaat
     5761 acgtcaaaaa aatgaaataa cgaatacgca cccactatct aaaagagagg tatcctatcc
     5821 ttcttttata gtgggtgatt tttataaaaa tagggctatt tcctcttgga atagctagta
     5881 tttgtagtta ttatacggta ttcttgaaat aagattagtt ttattatata aggagagtga
     5941 caaatggaat cacgtgaaaa aattgccgtg ctcattcctt gttataacga agaggcgaca
     6001 atttcaactg ttattgaaga ttttaaaagg gaattaccag aagcagatat ttatgtctat
     6061 gataataatt cgaccgatcg aacatacgaa ttggccgttc aaggcggagc aatcgtaaag
     6121 aaagaaccac gtcaaggaaa agggaatgta atccgtcaaa tgttttttga tattgatgcc
     6181 gattattatt tgatggtaga tggtgatgat acttatcctg cagaagctgc acatggtttg
     6241 cttgagaaat tacgttctgg cgaagcagat atggtcattg gcgatcgtct atcaaatgga
     6301 acctattttg atgaaaataa gcgtcctttc catgattttg ggaataatct ggttaaaaat
     6361 acaattactt acttatataa aacaaaaatt cgcgatgtca tgaccggcta tcgaggattc
     6421 aatcgaattt ttgtcaaaag cttccctatt atgagttctg gttttcaaat cgaaacagaa
     6481 ttgacgattc atgctttaga taaaaaattc aagttcgttg agattccaat cgattatcgc
     6541 gatcgtcctg aaggtagcga atcaaaattg aatacattct ctgacggatt caaagtgatc
     6601 atgatgatca tcaaaatgtg caaagattat aaacctttgc ttttctttgg gatctggacc
     6661 atcttcttct ttttatttgg attggtcatg gggattcctg tgattcgtga atatatgctg
     6721 acaagcttta ttacacgcat cccttctgcg attttaagta ctggattgat gactttagcc
     6781 cttctttcat tagtaacagg gttgattttg gatacggtcg tcacaaatgc caagaaagaa
     6841 tatgaattga atttatacca tgtctaccac gaatatcaaa ataaagaaat gaagtaggag
     6901 gcctttttta tgcataagaa aaaggagaat ctatttccta ctttttttgc aactttattt
     6961 gcgtcaggtt ttgctttttt tacttcttat ggtctaggaa ctttgaatcc aacagtaatc
     7021 attggtttag ccatggtttt gctacttgct tttaagcaat atggaataga gttgttttca
     7081 ataaaaaaca aagttgcagc tattgcccga ttgttttttt ctgcagcttt tgctcttaca
     7141 ctttctattg gcagacagat acaatttcag tatgtctcag caaactatac aaagacaacg
     7201 attgctgatg gaagtatttt
//
LOCUS       DAGCYL010000138         7201 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.140, whole genome shotgun sequence.
ACCESSION   DAGCYL010000138 DAGCYL010000000
VERSION     DAGCYL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.140"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            346..819
                     /locus_tag="MB216_002185"
     CDS             346..819
                     /locus_tag="MB216_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBT4526980.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            816..2948
                     /gene="feoB"
                     /locus_tag="MB216_002186"
     CDS             816..2948
                     /gene="feoB"
                     /locus_tag="MB216_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBT4526981.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2950..3084
                     /locus_tag="MB216_002187"
     CDS             2950..3084
                     /locus_tag="MB216_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBT4526982.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3178..3822
                     /locus_tag="MB216_002188"
     CDS             3178..3822
                     /locus_tag="MB216_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4526983.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            4009..5202
                     /locus_tag="MB216_002189"
     CDS             4009..5202
                     /locus_tag="MB216_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBT4526984.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5195..6922
                     /locus_tag="MB216_002190"
     CDS             5195..6922
                     /locus_tag="MB216_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBT4526985.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         2345 a         1250 c         1595 g         2011 t
ORIGIN      
        1 ttagtaaaaa agtcaaacaa caaacactac atattgtgtt gtggaaactg tggaatacta
       61 agttttgtgt ttttgaaaaa agagctgaaa acaagtatcg tgtgggaaaa tagcaaattt
      121 aatagagaaa aaattgtgga aaactatgaa gaaatatttt tttctcattt ttatctatca
      181 atactaaaga ttcgctcata atatcacaag tataattgac attctcaatt accgagagta
      241 ttctatgtga gaatgattct catttatttg cgtaacacgt aagaaaatta gaacatataa
      301 tgaatgattt taatacatga tttatttcac aagagaggaa tcaatatgca gaccttagcg
      361 caggcaaagc agaatttcgt ttatcaagtc gaacgaatcg aaacagaaga aaaaacaaaa
      421 cgccatttgc aaaatatcgg gatcgttgcc ggaagtaaaa tcgtcgtcgt caatttatca
      481 ggcaacaatg ccattttact ggtaaaaagt catcgagtgg cagtaagtcg ggaaatacta
      541 ggtcaaatct ttgtcaataa aaagactgct tcctctgaaa gctggacttc tcttgatcaa
      601 ttaaaagcag gagagactgg gatcgtcgta gcaatccacg gacaaggcgc agtgaagcga
      661 cggttaatgg atatgggatt gacgcgaaat gtaaaaatag ttatccgaaa actagcccct
      721 ctgggagatc caatcgaact gacagtgcga gggtatgaac tgacgttacg aaagtcagag
      781 gccgaactaa tcttgataca aagggaggaa aaagaatgaa tatcgcgtta gtcggaaatc
      841 caaacagcgg gaaaactagc ttgttcaatc tcttgactgg ttcgaatcaa tatgtaggga
      901 attggccggg cgtaacagta gaaaaaaaag aaggatcgtt gaaaaaaaat ccgaacattc
      961 atattcagga cctacccggc atctattctt tgtctcctta tacaccagaa gaagtgattg
     1021 caagagatta tttggttcag gaacagcctg aactgatcat caacattatt gatgctacca
     1081 atttagaacg aaatctttat ttaactacgc agttattgga tttagacatt cctatgatca
     1141 ttggtctgaa tatgatggat gtcatcaaaa aagaaggaaa aaaaatcaat ctagaaaagc
     1201 tttcctatgg tcttggtgta ccagttttac caatcagcgt attgaaaaag caaggaatcg
     1261 aaaaactaat cgaaaagatt caggagtctg caaaaaagcg gccttctatt cctgttccct
     1321 gtacatatga tccacgttta gaagcagctt tacatgaaat tgaagatgta ctaggagaac
     1381 aaagcggcaa gaaacgctgg tatgcaatga aatattttga acgagatcag aaggtaagag
     1441 aggattacga gatagcagtt tctcagcaaa aagagatcga gcaactgatc cagttaacag
     1501 agaaactatt ggacgacgat agtgagacga ttctggtcaa tgaacgttat gaatttatta
     1561 cccaactatg tacactctgt gttgtgtcaa atgattcttt ccaattatcg atgagtgaca
     1621 aaatcgatca gattgcaacg aatcgttggt tagccttgcc gatcttcgca tttgtgatgt
     1681 ggctgatcta ttatctggct attcaaacag taggaacaat ggggacagat tggatcaatg
     1741 acacattgtt tggtacatgg ttaccagaac atgttggcag attgttagct gaatggcaag
     1801 tagcgggatg gatgcaggag ctgatcttga acggcatcat cgcaggcgtg ggagcagtgt
     1861 taggatttct accgcaattg attatattgt ttttatgctt gagctttctg gaagactgcg
     1921 gatatatggc gaggatcgct tttgtcatgg atcgactttt caggaaattc ggtctttcag
     1981 gaaaatcctt cattcctatg ctgattgcta caggatgtgg agtgccaggc gtcatggcca
     2041 gtcgcacgat tgaaaacgag caagatcgtc gtttgacgat catggtcaca accttcatgc
     2101 cttgttctgc aaagttgccg attattgcct tagtagcagg ggcctttttt cctcatcaat
     2161 cttgggtagc tccttctgcc tattttcttg gtatgactgc aattattttc tctggaatca
     2221 gcttgaaaaa aacacgtttg tttgctggag atacagctcc ttttatcatg gaattgcccg
     2281 catatcattt tcctcaatgg tcagcggtcc taagacaaac ctatgaccgc agcaagtctt
     2341 ttgttaaaaa agcaggaacg attatctttg tttctagtat cgtgatctgg ttcagctctt
     2401 cttataattt ttatgctcaa cctgtttctg aagaccaaag tatcttagcc tttttcgggc
     2461 gaatcctggc agttgttttt cagccgcttg gttggggaaa ctggcaagga acagtcgcag
     2521 cgattacagg attggtcgct aaagaaaata ttattggcac atttggtatt ttgtttggtc
     2581 atgcggaagt atccgaaaat ggcagagaaa tctggcaagt gctacaacac acatatactc
     2641 ccgcagcagc ttattcattg ttagccttca atttactttg tgcgccttgt tttgcggcaa
     2701 ttggggctat ccatcgggaa atgaatgcca agaaatggac ttggatcgcc ataggctatc
     2761 aatgcggttt agcttattta gttagttttg tgatttatca gttgggacac ttattggagg
     2821 gtggacaggt agcgttaggg acgtatcttg ctattttact gatgatagga ctcgtttatg
     2881 ttttgttccg tcagccaaaa tccaagaacc agttttatac tatattgtca ttagaaggag
     2941 aggaatagga tggcgacatt cttattaagt gtgttaatct ttggaagtgc aggcgcaatt
     3001 gtctatactc gtttaaaatc cggtaagaat tgtgatgact gtcaaacagc ttgcccagta
     3061 aaaaaggaac agatcaagcg atgatcagtc gttgcaagag gctattctcc ccttttttgc
     3121 ttattttgct agagcatagt aagatgaggg aaagtaaaaa aagaaagagg aaagaaaatg
     3181 tctggaaaga aaaaaatcgt ccgcccgcgc taccagcaga ttgctgttga tttagccgaa
     3241 cgtatcgttg aaaaccgata taaagtgggt gagaagatcc atgcccgctc aacacttgcc
     3301 agtacgtaca atgcttctcc tgagactacc cgcaaggcta tcaatgtgct ggtagatctt
     3361 ggaatcatgg aagtacggca tggaagtggg gcatttgttg cttcgaagga aaaagcaaaa
     3421 gatttcctat tgcaatatca agatgtacaa tccatccaag atacaaaagc agaaatcatg
     3481 gcaagtgtgg agaagcaaca agatgagctg aatcattttt ctcaattatt ggatcagctg
     3541 gtcaatcaaa cgaagcgaat acataagatg aatcctttac ttccgtttga attatcttta
     3601 tcagaaaaag caatgcatct ggaccgttca attagcgagt tgaatatctg gcaggcgact
     3661 ggcgccacag ttatcgcaat ttctcataat gaagaactgc ttgtttcacc tggcccctat
     3721 gctaaattta cggcagggga catcgttcat tttattggaa atgaatatac actacagcgt
     3781 atgacaaact ttttttaccc ggagcatcag cttgcagaat gattgtgtga aatattaaaa
     3841 aaatattaac ttggctaaac cttttattta caaagattta gccaagtttt tttcagtttg
     3901 gacaagcaaa tttctttcat ttttgacaaa gtgactacat gatgttaaca tagtgtggtc
     3961 acttttttca gaaagaaaaa tgaatttaca taaaaaagga gaatttgttt ggtaaaagta
     4021 gaagtaaaac atttaacaaa gatatttggt aaaaagacac aagccgcttt agatatgatg
     4081 aatgatcacc agccgaaaac ggagatttta aagaaaaccg gtgcaacggt tggtgtgtat
     4141 gacgtgaatt ttgacgtaaa agaaggagaa atcttcgtga tcatggggct ttccgggagt
     4201 gggaaatcca cattgatccg tctattgaat cgtctgattg agccaacttc gggcagtatc
     4261 tatattgatg gagaagatgt atcgaagtta agcaaagaag aattgcgaga agtccgtcgt
     4321 cataaaatca atatggtttt ccaaaacttc ggtctgtttc cacatcgaac gatcttggaa
     4381 aatacagaat acgggttaga agtccgtggt gtacctaaag aagaacgaca agaaaaagca
     4441 gaaaaagcat tagaaaattc tagtttgctt tcatttaaag accaatatcc tagtcagtta
     4501 tctggtggga tgcaacaacg ggttggccta gccagagctt tagcaaatga tcctgagatc
     4561 ttgctgatgg acgaagcgtt ctcggctctt gatccgctga tccggcgaga aatgcaagac
     4621 gaattactgg acctgcaagc caatgttcaa aaaacgatta ttttcattac ccatgatttg
     4681 aatgaagctt tacggattgg ggacagaatc gccttgatga aagacggcga aatcatgcag
     4741 atcggaacag gtgaagaaat attgaccaat ccagcaaatg attatgtacg tgaatttgtg
     4801 gaagaagtcg atcgttccaa agtgcttacg gctcaaaaca tcatggtacc tgcattgacg
     4861 acgaatatcg aatctgatgg accaaacgtt gcattgacaa ggatgcgtaa tgaagaagtc
     4921 agcatgctga tggctgttga cagaaagaga cacctgaaag ggatcatcac tgcagatcaa
     4981 gcgttagaag ctaggaagca aaaacgtcca ttgatcgact tcctagatga aaatgtgacg
     5041 gtgattggga aagacatggt ggtaagtgat attttcaata ttatttatga ctcaccgact
     5101 ccacttgcag ttgtagagga tggaaaatta aaaggtgtcg tcatccgagg tagtgtgatt
     5161 gaagcattag cagaaacaag cgaggtgagt gaacatgaat aatctattga actatcaatt
     5221 accagtggca aactgggtag aaaatatcac agaatggttt acgacaactt tttctggcct
     5281 attttcattt ttacaaacga ttggacaggc agtgatgtcg ggaattacaa atttactgtt
     5341 agtgattcca gctcctttgt ttatcttgtt attgacggta gcagctttct ttatttctaa
     5401 gaaacgtcca ggacttacgt tgttcacatt aatcggtcta tggtttattt ataaccaagg
     5461 tctatggaat gatttgatga atactgtcac acttgttctt ttatctagtg tgatctcgat
     5521 catcattggg gtaccattag ggatcttgat ggcaaaaagc agtaaagccc aaagtattat
     5581 caaaccgatc cttgacttta tgcagacaat gccaggattc gtttatttga ttccagcagt
     5641 tgcatttttc ggtatcggga tggttccagg ggtatttgcc tcagtcatct ttgctctgcc
     5701 accaaccgtt cgttttacga atttagggat tcgacaagtt cctaaagaat tagtagaagc
     5761 ttctgattca ttcgggagca caagtcgtca aaaactattc aaactagaat tgcctttagc
     5821 taaaagtacg atcatggcag ggatcaatca aacaacaatg ctgtcacttt caatggtcgt
     5881 tattgcctct atgatcggtg cacccggctt aggacgcggc gtattatcag cattgcaaag
     5941 agcacaagtc gggaatggat ttgtcaacgg ggttgcatta gttatcttag caatcatcat
     6001 tgaccgtttt acacagcatt tgaaccagcc aaacaataaa aaagctgcag gagcaacaac
     6061 acagaaaagt aaaaaacgtc aaggactgat cattggtgct gtagtagtag tgatcttagg
     6121 tgcgattggt atagggagtt tttcatctgc caaagaaaca aaacgtatca acttatctta
     6181 tgttgaatgg gatacagaag ttgcttcaac aaatgtagtc ggtgaagtat tgaaacaaat
     6241 gggctatgat gtaacgatga cgccattaga taattcaatc atgtggaaat ctgtttcaaa
     6301 tggcgagtca gatgccatgg tttcagcttg gctacctaaa actcatggtt cacaatatgc
     6361 acaatataaa gatcaagtcg aagacctagg tgccaatttg acaggagcaa aagttggttt
     6421 agcagtgcca gcctacatgg atgtaaattc gatcgatgag ttaaccgatc aggcaggtaa
     6481 gaaaatcata ggaatcgagc caggagctgg tgtagttacg gctgctgaaa acacgatcca
     6541 aaaatatgac aacttgaaag actggaaagt agagacatct tcttcaggag caatgaccgt
     6601 tgcactaggt caagcgatca aaaaacacga accaattgta gttacaggat ggacacctca
     6661 ttggatgttt gccaaatatg atctgaaata cttagaagat cctgaaaacg gaatgggaag
     6721 cgaagagcag atccatacga tggttcgaaa aggattgaaa gaagaccagc cagaagctta
     6781 caaagtatta gacaatttcc attggtcaga aaaagatatg gaaaaagtta tgttggaaat
     6841 caacaatgga aaagatccac agcaagcagc aaaagactgg atcaaagaga accaagaatt
     6901 agtcgaaagc tggaaaaaat aaaaagacag acacgaaata aatggagctt ccaaaattgg
     6961 atcaaatatc caaatttgga agctccatgt tcgtctctat cttttttgtc tataaaaaaa
     7021 gagaatgcgt agctcatctt cgaaacgttc aaagacgccg acaaactatt atgaatttat
     7081 gggtgcgagt caaagagatc gatcgtaggt atgaaagact aatgtgatta aagtttatta
     7141 tgtacgaagt gcattctcaa aaaatattaa agaatataca tagtttatat acttaaaagt
     7201 g
//
LOCUS       DAGCYL010000139         7086 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.141, whole genome shotgun sequence.
ACCESSION   DAGCYL010000139 DAGCYL010000000
VERSION     DAGCYL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.141"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(102..620)
                     /locus_tag="MB216_002191"
     CDS             complement(102..620)
                     /locus_tag="MB216_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBT4526986.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(622..1239)
                     /locus_tag="MB216_002192"
     CDS             complement(622..1239)
                     /locus_tag="MB216_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBT4526987.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKFGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1236..2090)
                     /locus_tag="MB216_002193"
     CDS             complement(1236..2090)
                     /locus_tag="MB216_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4526988.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2087..2998)
                     /locus_tag="MB216_002194"
     CDS             complement(2087..2998)
                     /locus_tag="MB216_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4526989.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3039..4373)
                     /locus_tag="MB216_002195"
     CDS             complement(3039..4373)
                     /locus_tag="MB216_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4526990.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4581..5411
                     /locus_tag="MB216_002196"
     CDS             4581..5411
                     /locus_tag="MB216_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBT4526991.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5655..5937)
                     /locus_tag="MB216_002197"
                     /pseudo
     CDS             complement(5655..5937)
                     /locus_tag="MB216_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066964.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6095..6289)
                     /locus_tag="MB216_002198"
     CDS             complement(6095..6289)
                     /locus_tag="MB216_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526992.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6794..6979)
                     /locus_tag="MB216_002199"
     CDS             complement(6794..6979)
                     /locus_tag="MB216_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4526993.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT         2338 a         1409 c         1214 g         2125 t
ORIGIN      
        1 agttattaag acaaatgaat aaacaactac gctttcctcg aaacaaaaaa ataaagcaca
       61 aatgcgcatt tacgcactca tgctttattc gtacaattaa ttcagataac ggcagcgcat
      121 aggatgccaa taacacttcc taaaatagct cctcctaaaa tgtcagtagg ataatgtaaa
      181 agtaaaaaaa tcctcgaata agcgatcaaa caagcaaata aaaaggctaa tattcctgca
      241 actggactta attcgaacaa cgcagctact gataaaaagg ctagcgctgt atgtcctgaa
      301 ggaaaagaag aagtcttcgg tttacttgtg taaaggacaa gccgacgatt tttttcataa
      361 ggtcttttcc gtcttgtcgt ttttttgata aaaagcaaac caacaaaaaa atagatcaaa
      421 ctgccgataa gaatggctag accatattct ctagtatcag gtatcaagac taaaacagca
      481 gcaaggagca accaaataaa accaccatta gagatattac tgataaattt caaaaaattg
      541 atttttcgac tatccgtttt ccgttgcttg gcgaacttgt tgataatact atcatcgatt
      601 ttagcaatca aacgcaacat gttattcctc ctttttaaga accttttctt gcgtacgatg
      661 gataaaaagc aggacttgaa aataaaacaa gaaactgaaa cctaataaag caaaatagat
      721 tggtcctgca aaccagaatg cgacaagaag gatcaacgtg cctgccaaat agaaaagcgc
      781 gcttttgaaa tcattaaaaa acaagaaaaa tgcagtaatc caagaagctt ctttttgttc
      841 cagttgaagc aagattttgt aactaaagag aagcgcgctt aatagaagca aaaacaacgt
      901 agtatacatc aatgtatagc caattccccc accccaatag cggaaaatct gataatcgat
      961 caatagaaga agaaaataaa cacttgctaa actatcttgc tttccataaa gagagaagtt
     1021 ctcacgaaac tttttccaga atagtgaacc gaactttttc tcttcatttt ttttgtaatg
     1081 ataaatcata tacagaactg ttcgacttgc agttcctaat gtgaaaacag gcaagacgca
     1141 caggaaccat agtagctgta aatggataag acgtggaatc cattcaatga tttctaagaa
     1201 ataatcattg gcttttttac caatcgatcg attcatcatc cttttgaacc tcctgtcaaa
     1261 tccatccctt caatgaaata acgttgtgca aaaaagaaca gcaaaatcgt cgggatcaag
     1321 acaagtgttg ctcctgccat cagataattc cattgtgtcg ttgcttggtt ctgaaaaact
     1381 tgcaagccaa tctgtaacgt gtatttttct tgatcttgga tatataacag aggacctaag
     1441 aaatcattcc aagctgcgtt gaaggaattg atcgcaatgg taatcaaggc aggtttcgtc
     1501 aatggaagca tcaaatgact ccaaatatac aaatgattcg ctccgtcgat ttcagcagct
     1561 tcgatcaatt cattattgat cgataagtaa aattgacgca tcagaaatat attgaacgca
     1621 ttgccgaaga acgatggaac gatcaggggc aaataagtac ctacccagcc gatcttggag
     1681 aataatacat attgagggat catcgttaca aaccccggga tcatcatcgt agaaagaacc
     1741 aaagcaaaca tcagcttttt cccaggaaaa tcaagcttcg caaatccata agcaataaaa
     1801 gaatttgaaa ggacattacc gatcactact agcacagtga tgaataatgt atttcttgca
     1861 tagcgccaaa aaggaaaggc ggcaatcgta tccagatagt tttcccatac gattttttct
     1921 gggaaaaagg tcggaggata acgaacgatt tcttccattg gtttcaatga agtaaacacc
     1981 atccaaagca atggaatgat caaaatgatt cctattactg tcatgaggac ataacttagt
     2041 attttttttg tttgccgatt cacactggtc tttttgaaat aatccatcat ttgccgtcac
     2101 ctccagaata atagacccac ttaggtgcaa gcttcaaatt gacaaacgtc agaaccaaca
     2161 cgatcagaaa caggatcatt gacatagcca tcgcataacc catatcaaaa gctacgaacg
     2221 ccttgttcca catatgcaga ttataaaaaa gcaaagaatt tgcaggtccg cctgttccat
     2281 ttttcgtcat gacataagct tcttgaaaaa tttggaaaga accgatcgta gaggtgatca
     2341 catcgtaaaa aataatagga gtgattaatg gcaaagtaat acttttgaac tttccccaaa
     2401 cgcctgctcc atcgatttct gctgattcat ataaagatct aggaacattt tgtaatgtag
     2461 ctaagtaaag tagcattgct cctccagaac tccatatttt catcaaaatc aacgcaggtt
     2521 tcgtccattg tggatcaaac agccaagctg gtccagggat atggaaccag cccagaaatg
     2581 tgttgatcaa tcctgcagat ggagaaagca gctgcatcca taagatgtag actgctactc
     2641 ctgataagac tgccggtaaa tagaaagctg tacggaatac actgattcct ttgatttttt
     2701 gattgagtac tgtggctaaa aacacactga aaatcgtcgt cagcggtaca ttgaagatga
     2761 cgaaataaac ggtgttgtat aacgatttcc aaaataaatc atcgtgaacg aacattcttt
     2821 tgaaatttgc tagaccaatg aaattcatct tggatgtcaa attataatcg gtgaaactac
     2881 caataactga aaaaagcata ggccctagca taaataccaa gaacccgata ataaaaggcg
     2941 agataaataa ataggcccat cttgcttctt ttttctttcg gatagataaa cgcttcaaat
     3001 tttcttgcct cctctgtacc tagacagtca aaaatggatt attgattttg cttgactaag
     3061 ttttctactg ctttttgtgc atctgccaat ccttcttttg gtgattttgt tcctaaaaga
     3121 atctggtcaa tctgttcatt cactaatgtc gtgtaatccg gagcatctaa aggaactggt
     3181 gtgatcaccg tatttttaaa gttttcgtcc gccatttgat agatcatttt tcctttatcc
     3241 gttaggttgt cattattcac taaattctca ttggctgtct tgctcgccat gatatcaaaa
     3301 cttttttcgc cgaatttttc ttgtacttct ggagaagaaa gatatttgat gaattcataa
     3361 gaggcctcag gatcttttgc tccgtatggt acttccaaga cgaatccgcc accccacgac
     3421 caatggcctg aaccttcttc tttttctggg agctgcatca cgccaaaatc aaaatcatcc
     3481 ggtgcatttt ccatcaaact agagtaataa ttgatatttt gggcacgcat ggctactaat
     3541 ccggaaataa atggatcaga tacaccagac ccaaattcgg cttctaattt attgatcgta
     3601 tttttcccat aataatcttg ccagtcttgg atccactcca aggcttccac tttattttta
     3661 gtatcaatct tgactttttc atctttgtca aaccaactag tcccgttatc tgcgtttagc
     3721 gcccatacat cagcccctaa attccataat ggataaaaac cgatgcgtgt aaattctttt
     3781 ccttcttttt tatctagttt acgtgctgtt tcttctaatt catcccaagt tgtcggtaga
     3841 tcttgttcac tgattccagc ttctttaaat aactttttat tataaaaaat gacttgtgta
     3901 tctgtattga atgggatcgc ataaggatca ccttcatatt ctactgtatc ccaaagctgc
     3961 ggataaaaat ccttagaagt gtctttttct acatatttgg agagattagt tgcttgctgt
     4021 gcttctgctc tttgggcaac ggaatttatg tcctgtacaa taacatcggg tgcatttcca
     4081 gctgttattg cagataaaga tttagtccag atgtcacccc atggctgata cttataattt
     4141 acatggatct cgttttgtga ttgattgaaa tcctcaatga tttcttcgat agcttctttt
     4201 cgtgcaccgg atccccaaaa ggaccagaaa tcaatatttt ttcttccatc acttgttttt
     4261 tcagaagaat ttgctgcact tgaactggca tcgcttgcat tacttgatcc gcatcctcct
     4321 aataaaaaca atgctgaact tgccagcaac cctgtaaaca ataatttttt catattttac
     4381 ctccaaattt taatgtaacc gatgccggtt ttaatataac gatatcattt tgttctgaaa
     4441 ataacaattt ataaggtatg attctaaaaa aattattaat gataccaata ctttatataa
     4501 ttcgcgagcg ctttctttga attttttaat ctttcgtagc agaatgagaa tgtattaaaa
     4561 atagattagg aggaattttg atgtttagac accagaaaga attgcagttt gaagcaaaac
     4621 cagatcgtcc gaatccatta atcgcgaaat atctgcagga gctgatcggc ggccaatatg
     4681 gcgagatgag tgtagctatg cagtacctgt tccaaggctg gagctgccga ggagaagaaa
     4741 aatataaaga tatgctgatg gacattgcaa cagaagaaat cggccacgtg gaaatgttgg
     4801 cgacaatggt tgctcgttta ttagagggtg cgcctgttga agatcaagaa gcagcgatgc
     4861 aaaaagatcc aacaatcggt gccattatct ctggaatgaa tccacagcac gcgatcgtag
     4921 ctggcttagg acctcgtcct gctgacagcg tgggcaatcc atggagcggt gcctatatca
     4981 ttgcgagtgg caacttgtta gcagacttta gagctaattt aaatgccgaa tcacaaggaa
     5041 gacttcaagt agcacgtatt tacgaaatga tcgatgatcg tggcgtaaaa gatatgctgt
     5101 ctgttctcct tgcaagagat agctatcatc aaaatctatg ggaatgtgca gtgaaagaat
     5161 tagaagaaaa agaaggttca atcttagtcc caagtacttt cccgcgtgac aaagaacgaa
     5221 aagacattgc ttatgacttc tataacttct cagagggaga ccaaagtagt gaaggttctt
     5281 gggctagtgg caaagcactg gatggcgaag gcgaatatca atggttgaaa gaacctaaga
     5341 tcgaaggcgc tgcaccaaaa ttagaaccag taacaccaaa aatgtatggg acacctccat
     5401 tcaagaaata aatatctacc cgtagaaaca cccgtttttt gttccacacg ctttgtgagc
     5461 aaaactgact aaagtcatta atcataacca cccttcaaaa gggtggtttt ctctagagct
     5521 ataagctcaa atcaccggcc agcgcctaaa gacgctggct ttcatatttt atgttcaagc
     5581 catattgcct ttgccagttt tgcccacctc ttaaaaggtt tatgtactac tccttagccc
     5641 ttaaaaggat cgtcttactc tcgtacactc agcttatcta acgcactatc atgtttttct
     5701 tgttcttgaa tgtacttttt gatggtcgct tcattttgtc ctacttttgt cacatagtat
     5761 ccttccaccc aaaagtgtcg atacccaaat ttatatttta gatttgcaca atctcttgat
     5821 gatctcctga atattggctc gatattgatt ataaattatt tttcttctat actttggtgt
     5881 gaatacaatg tggtatttac acatctactt tgtacgggct aaactatttc cctttattgc
     5941 tccacccgca tagcaagtgg ttttgtattt caatcgctcg gcgcgctcaa ttaggtctaa
     6001 agacaataaa tctaaaaacc cgctgcttca ctgacagaaa caacgggttt tcagaaaaaa
     6061 atgaaatgaa aaagatctct tacggcaatc tattttattt ttttgatttt ttataaatgg
     6121 aaaatgcttc aagaagtcca agaaataaag ccgtataaaa acaagaacct atgaaatctt
     6181 tatttattag atattcaaca gaaatcccga taacactagc caacaatata tttatcagaa
     6241 gtgtaaaata aggatgcttt ttatagaata gaactagtgt tgttttcata gactctcctt
     6301 tcttcatatt tccaattaat cacaaattca cggcattact agaaaaatta aaggggcgca
     6361 gaagtggaaa aatactcttc gactttccct acaaaccagg ccatcttgtg tttaataact
     6421 ataccaaatc taaggtacct ctaaatctaa attccatttg tggcggtgac ctccaatttt
     6481 taactcttct atctcccatc cagtatacat aaccttgata cttcgaccac ttatatatcg
     6541 aacacacaaa tacctatcca ttgcaggagt actacctaac ttagttgcta cttgcatcta
     6601 aataacagct gaaaagatag gacttactct tagtaaagta tctgaactat taatttttaa
     6661 actatcattt ttatcctcaa ctacatgcac atctgttgca acttcacttt ctgtctcatc
     6721 cagataaatg attaaggaat cgggaattgc aatatcttca gctgacgctg gaatagttga
     6781 gctaaattag tcatcatatc tttattataa aataaccaac agcaaaaaat acaatgaaaa
     6841 acaaacttgg aactaatgta tcattaataa atgttgaaaa attcagcaac tcattactgt
     6901 atgattttag cagcccaacc aacatatacc ctataaaagc taataagatg gttaggaatc
     6961 ctttagaatt tttactcatt catagacact tccttaaata acttttatta tagaaggtat
     7021 tgtaaataat aatattgaca aaaagtgact caaaataaaa aaaaccttac attatactgg
     7081 aatgaa
//
LOCUS       DAGCYL010000140         7022 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.142, whole genome shotgun sequence.
ACCESSION   DAGCYL010000140 DAGCYL010000000
VERSION     DAGCYL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.142"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            177..1034
                     /locus_tag="MB216_002200"
     CDS             177..1034
                     /locus_tag="MB216_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBT4526994.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1027..1929
                     /locus_tag="MB216_002201"
     CDS             1027..1929
                     /locus_tag="MB216_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBT4526995.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1962..2312
                     /locus_tag="MB216_002202"
     CDS             1962..2312
                     /locus_tag="MB216_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBT4526996.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2361..3283
                     /locus_tag="MB216_002203"
                     /pseudo
     CDS             2361..3283
                     /locus_tag="MB216_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            3361..4254
                     /locus_tag="MB216_002204"
     CDS             3361..4254
                     /locus_tag="MB216_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4526997.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4292..4855
                     /gene="amaP"
                     /locus_tag="MB216_002205"
     CDS             4292..4855
                     /gene="amaP"
                     /locus_tag="MB216_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBT4526998.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4869..5057
                     /locus_tag="MB216_002206"
     CDS             4869..5057
                     /locus_tag="MB216_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBT4526999.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5070..5972
                     /locus_tag="MB216_002207"
     CDS             5070..5972
                     /locus_tag="MB216_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBT4527000.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6004..6246
                     /locus_tag="MB216_002208"
     CDS             6004..6246
                     /locus_tag="MB216_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBT4527001.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6502..6873)
                     /locus_tag="MB216_002209"
     CDS             complement(6502..6873)
                     /locus_tag="MB216_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527002.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2567 a         1189 c         1393 g         1873 t
ORIGIN      
        1 acctttgaat aaaaagccat gaaaaacgaa tagttagcgc ttaccgatgc gttttgtgat
       61 aaaaaaattt taaaatattc aatgaaaaca aagcatatat tttgctttaa aaccaaatga
      121 ggaatattct agatgtagaa caggttagaa aaagattaga aaaaggagtg attttgatgg
      181 agaagagata cggcgtagtt cctgtggtaa ctgcttcaga cgaaaattat gctccttact
      241 tgagcgtgat gatcgcaact gcattagaaa attgcaataa aacaagacgc ataaaatttt
      301 atgtgattga cgatggtcta tcagaatata gtaaagaagg attggaagaa acggtaaata
      361 aatattcaag taatgcaagt atccaattcc tgacagtgga aaaagatatc tacgaagact
      421 tcctagtcag tgaccatatc acaactaccg cttacttaag aatttcttta ccaaacttat
      481 tagctaaaga ggattataag aaagtccttt acttagattc agatgtgtta gtactagacg
      541 atatcgtaaa actatatgat gagccgttaa atggaaaaac aatcggcgct attatcgatc
      601 caggccaagt gaaagctttg gaaagattag gaatcgattc cgatgacttg tacttcaatt
      661 caggagtgat ggtgattgac attgatcaat ggaataaaaa agaaattaca gaaaaaacta
      721 tccattacct cagtgaaaat ggcgatcgaa tcatctatca cgaccaagat gctttgaatg
      781 ctgtacttta tgaagattgg gaacaacttc atccaaaatg gaacatgcag acttcgctga
      841 tttttgaacg tcatccagca ccaaatgaaa aatatgaacg tcagtataaa gaaggaaatg
      901 aaaaaccttc gatcgttcac tttacaggac atgataaacc ttggaatacg ttgaaagatc
      961 atccgtatac caatttatac ttaaaaaaat tagcacacag tactctaacg aaagtaggcg
     1021 agatcaatga ataaaaaaga aatagcagta gtcgcaagtt gtaacacaaa attcgtacca
     1081 catttagctg cattatttgt ctcagtatta gataattgca acccgtctaa atttgttcgg
     1141 ttctatgtga ttgacgatga tatcgatttt gaaagtaaac agcttttacg cttctcagtg
     1201 aaaaacgcac gaatgaactc agatgtggaa ttcttgaaaa tcaataaaga attctttaca
     1261 aatgtggtaa tcagcgatcg tattccagaa acagcttatt atcgtattgc gattccggaa
     1321 ttgttccgcg gcacagaagt tgaacgtatc ttatatatgg attgcgacat gatcgcttta
     1381 caagatatct ctaaattatg gcgtcttgac ttcggtgatt caatcgtagc agctgttgaa
     1441 gacgctgggt tccatcaacg actggaaaaa atgaaaattt cagctaaatc aatgcgttat
     1501 ttcaactcag gtttgatgct gatcaatgtg aaaaaatggt tagatgaaaa tattacgcaa
     1561 aaagtattag atttcatcga acataatcca gaaaaattac gattccatga ccaagatgca
     1621 ttaaatgcta tcttacatga ccgttggtta ccacttcatc cgcgttggaa tgcacaaggc
     1681 tatatcatgg caaaagcaaa aaaacatcca acagcagcag gtgaacgtga atacgaagaa
     1741 actcgcaaca atccttacat tatccatttt tcaggacatg tgaaaccgtg gagcaaggat
     1801 tttgaagggc caacgaagaa atattacgaa aaatatgcag ggatgacggc tttccgttgt
     1861 gtagctaaat tccctaaata tccaaaatat gcaaaagttc aaccaaaaac agatgtagtt
     1921 gttaaataat agaagacaac aactgaagag ggggagtagg aatggaaatt acaatgtcta
     1981 aaaatgcagt agagacgtta attgaaaagg ttggggaaaa tacaaaaatc gcactcgcgc
     2041 ttatcaatga ttccgatcca tttctaaggg acaaaggtgc ttttgcaaaa ggcagctttt
     2101 ttcaaatcat tccttttgtt tcggaatttg gagaatatgc gactaaaatc gagcatccat
     2161 tattggacat ctatacatct aaattagagc aaaattattt cggtaaacga ttgaacatgg
     2221 acttcaacaa acagcttgac agcttttcat tagaaaatga aatcgcagta ctcgattata
     2281 atatcaaatt aaagaactgc ttcttcagct aatagtttcg agaaagtcca aaaaggaggt
     2341 atacattgag atactacttt gtggatgaag aaaagaatgt aatagaaaca gatgaaaaaa
     2401 aatataattg gctcgtactt gatagtgcaa agcccgaaga gattgagcaa gcaatcgaag
     2461 aattcgagct tcctgatgat attttcgtag gaaccgaata tccggaagaa gtttctcgga
     2521 tggagcgcct ttatgagacg aaactagagc atccattttc tttggtagtg attaatctag
     2581 atcagtcaga taatcgtatc gaaaaaaatt aacgccaata tcttttgtac tttccgataa
     2641 tctcttgatt acttgtatcg atagaaaaac cgaatttgtg gatcggctgc tgacaaagca
     2701 tggaaatcat ttaaactcgt ttgaaaagat catcacgtat acattattag atatttacac
     2761 ccattacgtg aaagaactaa gagagatgaa aaagcgaatt gacgctttgg ataaagcagc
     2821 tcgtaaaaca acggaaaatg aagaactgta taaacaggca gatcttgaaa gagacatcgt
     2881 atatatcgat cataccttaa gagatcaacg ggaaacaatg gatcaattat ggaattctga
     2941 aactttcatc aaacgtttag acgatgaaca gctactctat gatgtccatt tgagacagcg
     3001 acaaacagaa aaaatgatcg agatctatcg agatttatta gagagtattg gtgatctatt
     3061 caacgggatg atggacaata atctaaatca tatcatgaaa tatctagatt cagctgcatt
     3121 aatcatttcc gttccggcgt tgttcgcagg gatttgggga atgaatacag gaggattacc
     3181 gggtaaaagt tctgctctag gctttatact cgtagtcttt ggctctttga tagcagcagt
     3241 agttttaggt taccatttat accggaaaga ttatacaaat taaagaatct atttaaaaca
     3301 ggtatcaaac aaagaaattt gttttggcaa tacaatttaa aaactaggag gaatacagca
     3361 atgacagaac caaaattaat cgacccgcga aacttgtacc atacggagaa atttcctaaa
     3421 caagatcagg atacaccagc tttacagcag aatatgattc ctgttcctga ttgtggagaa
     3481 gaatcctatg aaggcaataa tcaactagaa aaccgccgtg tactaatcac tggtggagat
     3541 tcagggatcg gtcgtgcagc agcgattgca tttgctagag aaggggcaga tatcgctctt
     3601 caattcttcc caggagaaga aaaagatgca gaggaagtag caaaatacgt gaaagatgca
     3661 ggacgtaaga ttgtcttact accttttgat cttcgtgacg aacaagcacc taaagaaatc
     3721 atagataaag ccgtgagtga acttggcggg ctagatacga tggtactaaa cgcaggacag
     3781 caaatttctt gtcagtctat tgctgagtta ccacttgagc aagtcaaaga cacatttatg
     3841 ataaacatca tcgctatgtt tgctttagtg aaagaagctg ttccgcatat cccagcagga
     3901 ggagcaatcg taacaacaac ttctgttcaa gcattcaatc caagcgaaca tttattagat
     3961 tacgcagcaa caaaagcttc aatcgctaac tttacgatcg gtttagcaaa acagcttgca
     4021 cctaaaggaa tccgtgtaaa tggtgtagca cctggtccaa tctggacacc attacaacta
     4081 gacgaaggac aaccttctga ttcgatccct gaatttggtc agcaaagttt actagaacgt
     4141 gcaggtcagc caggagaatt agcaccagtt tatgttttct tagcatcgaa taaagcaagt
     4201 tatgttacag cccaaattta cggagtaact ggtggagaag cgatcaactt ataagaagtt
     4261 gatcgtttca cccctatgag gaggtgcgaa aatgaataaa ggtgtaaaaa cgatcggagt
     4321 gatcggaaca ttacttttgc tatctgtgct tttgtttgcg atactattaa gtgcaaccta
     4381 tccattgcct tttcagctag agaagtttcg attcttttcc atcactaatt catacgtgca
     4441 acaatatgta ttttggcttg ccgttgcatt tgctattttg acaattatca cgttacttgt
     4501 tttcctgttt tatccaaaaa caatcggaaa ctttctactg aaacatggtg atggggagtt
     4561 gacgttggat aaaaaagcaa ttgaaggaat ggttcgttct cacttgcatg aagatgaatt
     4621 catccattca ccaaaagtaa atgtcaaagc aacgaaaaac aagatcgccg tcaatgtaaa
     4681 aggggagttg aaacgaactt cagcactcat tggtaaaaca ggaacattga tgaaagaaat
     4741 cgaaaatgaa gtacaacaca tattgggaac aaaagagcca gtgaaagtat cggtgaaata
     4801 cacaggcttt gaagaaccag ctaaacgaag caatgaccgc aaacgagtag aatagggggg
     4861 gaccaaaaat gaaagaacta ttttcgacat acaagctccc tatcatcttt ggggtacttg
     4921 gattgatatt agccgtttta tggatcaccg tgggcttttt caagacctta ttgatcttga
     4981 tcatggcagc gatcggcgtc gctatcggtt tttatctgaa acaaacgaaa atcttagatc
     5041 actattttaa atcttaggag gaaacatcca tgaataacaa taacaaaaat aataacaata
     5101 aaatgaacaa ccaaaaacca gtgtctgcac atccagaccc aacgacacca aaccaatcaa
     5161 atggtgtgaa cgatgaaaag aacaaaacag caccagtatc tgcacatcca gatccagtag
     5221 tccctaacgt tcaacaagga gtagaagaga agaaacatga tgcagaaaag catgcacctc
     5281 atcatccagg aactccagaa tctaaagttc cagaacaaca tgatgcaatc gatccttcta
     5341 aacctgtgga agagcaaaaa catccttctg accatgcgaa agcacaagaa aaacttagtt
     5401 ctgaccacac aaacggacaa ggaaaacttg gttctgatca tacaaataac caaggaaagc
     5461 atgattctga acaggaagat tcaatgcgcg gtgagctgaa ttatgaagat aaagttgtgc
     5521 aaaaaatcat cggaatcgca attgaacaag tagacggttt attatctgca aacggtggtt
     5581 tcttctctaa tgtggcagga aaacttgtga atacagataa tgtaacggct gggatcgaaa
     5641 cagaagtcgg taaaaaacaa gtagcagttg atatggatgt cattgtggaa tatggcaaag
     5701 atatcgaaaa aatctttgaa gaaatgcaag aagtaatcgg caaagaagta aaaaatatga
     5761 cacatttaga agtgatcgaa gtcaacgcta acgtagtgga catcaaaaca aaagaagaat
     5821 ttgaaaaaga tcaagaaacg gtgcaagaca aagtaactga cgcagcgaaa aaaaccggtg
     5881 aatttgcttc gaatcaaaca gataaagtaa aatcagcagc tggaagcggc gcacaaaaag
     5941 taaaagaaaa tacagaacca cgggttcagt aataaggaag taaaacatta ggaggaacga
     6001 aacatgggat ttatttggtc acttatcgtc ggaggaatta tcggagcaat tgcaggagca
     6061 atcacgaata aaggttcttc gatggggatt attgcaaatg tcgtagcagg tttagttgga
     6121 tcagcacttg gtcaagcatt attaggttct tggggaccaa gtattgcagg aatggcaatc
     6181 gtgccatcat tgattggtgc catcatttta gtactaatcg tatcattctt tgctcgaaaa
     6241 ctgtaaaaag acttttaaaa agaggtcgtg aaaaagctgt tcttcatcaa gtaataagaa
     6301 cagccaaaca aaaatagctt ctcatatttt tcatttggtt gggcatatta tcgaagatgc
     6361 aagcttttga acactgttta tacggaataa gaggctgtga caagactttt gtcacagcct
     6421 cttatttatg cgggcaaatt cccaattaga tttttaaaag tttttcctta aatgtgtctt
     6481 tattgataca gagaagcttc tttaataaaa caatgcctcc ctaaattttc ttgagatagg
     6541 aaaggtgaac atacaaaata atacgaccac tttgaaaacc cagatgatcc acaaatggat
     6601 cgtatgatac tgatgaagta aaaagcaatg gatcagaaac tcaaagagat tgaaaaagac
     6661 aaggaagaaa taatatctct tttttactct tgtctgtttt agccaataat agctcgtaaa
     6721 gtaatctaga caaacaaata gaagtaaaca aagtttcgta agagcagcaa tcgtatcatg
     6781 ttgctctgct agacgattga cgagcaggaa aaatgttggt aatggaatat agatcacgag
     6841 taatgaaaga aaaaagtaaa caataaatgg catactattc ctcctaattg tgtgagttcg
     6901 ccctcatttt aaaataacga tatttgtttg tgaagctttt tcgtgtcgta acaaatgtta
     6961 tctaatgtat ttttaaataa ataatcatta ttatcaaaat aatgttaaaa ataaaaaaga
     7021 at
//
LOCUS       DAGCYL010000141         7009 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.143, whole genome shotgun sequence.
ACCESSION   DAGCYL010000141 DAGCYL010000000
VERSION     DAGCYL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.143"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(576..1592)
                     /gene="tsaD"
                     /locus_tag="MB216_002210"
     CDS             complement(576..1592)
                     /gene="tsaD"
                     /locus_tag="MB216_002210"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBT4527003.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1589..2053)
                     /gene="rimI"
                     /locus_tag="MB216_002211"
     CDS             complement(1589..2053)
                     /gene="rimI"
                     /locus_tag="MB216_002211"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBT4527004.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2060..2602)
                     /gene="rimI"
                     /locus_tag="MB216_002212"
     CDS             complement(2060..2602)
                     /gene="rimI"
                     /locus_tag="MB216_002212"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBT4527005.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2586..3308)
                     /gene="tsaB"
                     /locus_tag="MB216_002213"
     CDS             complement(2586..3308)
                     /gene="tsaB"
                     /locus_tag="MB216_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBT4527006.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(3499..4479)
                     /locus_tag="MB216_002214"
     CDS             complement(3499..4479)
                     /locus_tag="MB216_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4527007.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4503..5987)
                     /gene="galT"
                     /locus_tag="MB216_002215"
     CDS             complement(4503..5987)
                     /gene="galT"
                     /locus_tag="MB216_002215"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBT4527008.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(5999..6988)
                     /gene="galE"
                     /locus_tag="MB216_002216"
     CDS             complement(5999..6988)
                     /gene="galE"
                     /locus_tag="MB216_002216"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBT4527009.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         1895 a         1546 c         1248 g         2320 t
ORIGIN      
        1 atcagggtca ctgcttattt tcctccctaa aacttctgaa agatccggca gttttagatc
       61 actggacaag actcaaagat tttctatgtg tgtttcagtt atgtaatatt cactgtttgc
      121 aaaaaatact gttagattaa ctagatgaag cgtcttgtcc tcatccgatc tagattattc
      181 gatattcctc gacaataaac gattactcag tgagggcaat cagaggaacg gataatgata
      241 aataagctgt aaaaaatagc aaaataacaa taatgattaa aagtatgtaa aaaatagggc
      301 tttgacaaaa acgtgtcaca gccctatttc gattaaacgg tgtgtacctg tcaactcttc
      361 ggtcacaagt cataatgttc ataaacatga ggagctgttt tttgaaattg ttccttgtga
      421 atcagagtta aacgacaggc ttacgaactc ttgattaaac ggcgttcaaa agcttgcata
      481 cgcggtaata agaacagcca ggtcaaaaac atgagaagcc gtttttcctg ggctgttctt
      541 attacttgat gcagggcagc tttttcacaa cctctttatt cagataaaac caagcttgga
      601 tcagcattca actgatagct agcaaaattt cttttttgca tttccacatg tgctgctgct
      661 ccaatcattg cagcattatc tccacaaagt cgaagagggg gaatcactag ttctacctca
      721 gggagtttag ttgccaaagc atcctgcaat ccttcacgca acccttgatt ggccgctaca
      781 cctccagcta taatcagttg cttgactgga taattttgac aagctcgaag tgttttagta
      841 atcaatacat cgatcacact tgcttgaaag ctagctgcta aatcattttt gtctagatct
      901 tctccacgct gttgtgcatt atgcaccaaa ttgataaatg cactttttaa tccgctaaaa
      961 ctgaaatcat aattatcttc atggatcatc gctcgcggaa aatggtaatt atcttttccc
     1021 tgatgagcta gctgatcgat ttcttttccg cttgggtaag aaagacctaa tactcttcca
     1081 actttgtcat aagcttctcc tgctgcgtca tctcttgtct ccccaatgat ttcatatgat
     1141 ccatcttctt gcatatagac taattctgta tgtcctccgc taacaagaag tgccatcaat
     1201 ggaaactgga atggtttaac cagacgggct gcatagatat gccctgccat atggtttaca
     1261 ggaataagcg gcaattggtg tgcccaagca aaagccttag ctgcagaaat accaatcagc
     1321 aacgagccga caagtcctgg tccataagtg acagcgactg ccgataaatc ttctgccgac
     1381 acaccagctt ctaccagtgc atcttccaag cataaggtga tttgttctac gtgatggcgg
     1441 ctagctactt caggtactac gccaccaaaa cgtttatgac tgtttatttg agaagcaacg
     1501 atgttagata agatctctgt tccatttcta acgaccgcta cactcgtttc atcacaactt
     1561 gattcaatcg ccagaatcaa ttcttcattc atttcatcgt ctccgttttt aactttctgc
     1621 tcataatgat cgcatcttcc gtcggattgc gataatatga tttccgttta cctaatatac
     1681 gaaacttttc tgattcataa agttttcttg ctgcttcatt agatagacgt acttctaaaa
     1741 aaacatgcag cgaatctttt atttgttccg taatcagtaa ttgtaacaaa tgccgtgcat
     1801 gcccttttcc ttgttctgaa actgcaacag caatatttgt gatctcgact tcatctatta
     1861 ctttcgagta tccaataaag ccacagatct ggttatcttc agctaaaagc agataatcag
     1921 agtgaggctg ttggatgtct gccaaaaact gcattttcgt ccaaggtgaa ccatgtggat
     1981 aagcattttc gctaatcatc catacgcatt ctgccaagta cgcttcatca aaatcttttt
     2041 ttctgcaaat caactaatct cactcatttt ctaaaagttt tatcatatcc aacgcatctt
     2101 cttttgtttc agtgtaatat ctttttttga tcgttgttgc ttgaaacccc aatttccgat
     2161 aaagtctttg tgcatcttga ttactcatac gcacttctaa tgacatcgtc tcacagcgat
     2221 tcattatagc aaagttctcg atttcgtcaa taagcagcga accgattcgt ttacgctgat
     2281 atgccggtcg aacggcaata ttcgtgatat ggcaatctaa accgaacgct cttgaaccga
     2341 taaaaccaat cattcttcca ttatcaaaga tacctagata tagatgagga agcggtgact
     2401 tcaattctga tataaaggca ctttttgtcc atggcaattc gccattatag acttctcgtt
     2461 cgacatccag cagctctttg atatcttctg tagtcaattc tcttagtacg taacgacgat
     2521 cgatttgttc gactgttttt tctgtataaa tcattttatc ttgaaaaaag cttctggcat
     2581 acgctctaaa ttttttcaac ataactttcg tctccaggtg tgtgcgtctc caaccatttt
     2641 tcttctgctt ctactcgttt taaatagcga ggaagaaaag cttgtttctc tgcaactggt
     2701 tcaactaaag cacctaattg agccacagtt acaccacttg gaatatccca tgctggaact
     2761 gtattaatca tcgcactagg caatatttcc tttatttcag agaagaattt gtgacaatca
     2821 ctcccgacga aataaatagc ttcattcagc ttttgtagtt gtgtgaacaa atcagaagca
     2881 gaaatatgta catcttcaag tacagtttct aatctatttt ctttccagcg gtatgcccca
     2941 gcgtatacat tttttcttcg agcatcaaac atgggcacaa taatattatc cacaccgatg
     3001 caattagccg cgatggtttt cagactagat actcctacta gctctttatc taatgtataa
     3061 gcaatcgttt ttgctgtcgt tacacctata cgaagccctg tataagaacc gggaccatca
     3121 gaaaccgcga ttcggtcaat gtcttttgga cttatttgaa gatctgcaaa caattggtcg
     3181 attgcaggca tcagtgttac actgtggttg cgtttgatat ttgtttgtat ttgtccaaga
     3241 atttgttcgt cttctaccac accgatcgcc aaagtttggt ttgcagtatc tattcctaat
     3301 gtaatcatat atttgatcct ttctgctatg acattctcgc cttccattgt agcatacttt
     3361 tttattctga ggatgaaaat accaaaggca aaactagtac aataatacat gattatctgc
     3421 tggcgggcaa attttgcccc ttcctgaaac acaaaaaagc tgcggtatca ctccattttc
     3481 ccacagcttt tccatctttt atttcgttga ttctcgaata atcaactctg tttccacaat
     3541 aatctttctt gaaaccggtg ctttggaatt acttaattga agaatcgtct ccactgctag
     3601 ctctcccatc cattcggtat aaacttttac agtagaaagc gagggcgtta catatttcgc
     3661 tacactgaca tcattgaagc ccatcacaga aatatcttct ggtaccacta aacctttttc
     3721 ttgaatcgct ttcagtgcgc caatcgctaa tgcatcatta gctgcaaaaa gcgcatcagg
     3781 aacatgcgga ttcgttttta aatactgctt cactgcttga tatcctgact cgacagaaaa
     3841 atctgcttcg ataattggac cttcgtcgta gcctttttct gccaaaactt gttgaaatgc
     3901 cataattctg ggatctttta gccgctgatg gtttttcttc gtatattctg ttccagcaag
     3961 catagcgata tgttgcgctt tatgggagac aaagtgttca atgacttttt gaacacttga
     4021 cttaaagtcg acaactagag agtcgattcc ttgagaagtg ccgtcagaat ctaccaaaag
     4081 caaggcattt tgggatcctt tcatttctaa taactgagct tcgtcgaatt ttcctagtgc
     4141 gatcgttcca tctgccggaa gatcactaat ggaatcccac gtttctttca ataaagtaac
     4201 gtttgcttct tctgcctttt tttcgattcc taggcgaata gataaataat aaagatcttc
     4261 aagctcttcc gtatcgtcgt accactggat caggcgaagc gtttgcttag tatttgctgc
     4321 atttttacga tgtttcgtat aatttaagcg ttctgctgct tcaaaaattt tttgctttgt
     4381 ggctaagcca acggaaagat caggatcata gttgagaact cttgaaacag tagcaggaga
     4441 aactcctgcc aactgcgcaa tgtctttaat tgttgccatt tgtctctcct ccttttttac
     4501 gtttataaaa tcgcagtaaa tcgttcaaac gctgcctgtc ctttttcatc tcgcttgaat
     4561 actccagcgt cttccagtac acgtgcaaaa acctgaccta cagatttttg tacgacatcg
     4621 gtgaccgtat ctttcgtcaa atcaggatgt tcttttttta attgttctgc ccacggacga
     4681 tgatattcag ccatctggtt ttcttctcct aataagaaat gctctacttc tactagttct
     4741 ggtttcaaac gaggtggtaa aacagcaagc cccattactt cgatcaaccc aatgttctcc
     4801 tttttgatat gttggacatc tttatgtgga tgaaaaatcc catcaggatg ttcttctgaa
     4861 acattgttgt ctcgaaggac aaggtccaac tcaaagtaat tgtcccttct tcgtgcaatt
     4921 ggcgtaatcg tatggtgtgg tgtaccatct gctgaaaaag ctctgataga tactgtttca
     4981 tccgaatatg ctttccattt ttccaatact gagttagctg cttcaataag caagtttttc
     5041 tctgttgatt gcaaacggat cacagacatc ggccatttca cgactcctgc gatgacttgc
     5101 ggataatctt ctagtttgat aagtttttct atcggtgctt ttgccatagg aaattcatga
     5161 cgtcctgcct gataatgatc atgagaaaga atcgatcctc caacaattgg taagtcagca
     5221 ttcgaaccaa caaagtaatg aggcaagact tcagtaatcc gaagcagtct agcaaatgtt
     5281 tcctttgtga ttttcatcgg gcggtgttgt tctgataaaa tgatcgaatg ttcattgtaa
     5341 taagcataag gggaatattg gaatccccag ctttcaccgt ctagattcat tcgaataatc
     5401 cggtgattgg tgcgggctgg atagttcaag cgacctctat atccttcgtt ttccatgcaa
     5461 agcatgcagg taggatagtc tacttttacc gcctcgcgtt ctgctgcgat ttgttttgga
     5521 tctttttctg gcttagataa attgattgtg atttccagtt caccatactc cgactctgcc
     5581 ggaaatttga tgttttttgc gatggctctt gttttgatgt agtcattttt cttacttaaa
     5641 ttaaagaaat actcagttgc ttcctctggg cttttcgaat agtgctgtgc aaagaatgca
     5701 ttgacgactg aaggtggcgg tgtgagaaaa tccattaatt gcgcttcaaa aatctcttgt
     5761 tctgctggca attctgaaag aacaccattt tctcttgcct tagcgacaag ctgatccaat
     5821 aagtctacag attcttcctt aactggtctt acttcaatgc tttctagagc atcttctcct
     5881 accatcccta aaagacgatt ctgtaaatat aagcggtcca tttccatcca tccgccagct
     5941 tcgatcgcaa gtgtggcaaa atcacaaatc gtttgactga ttgacatatc tcattcccct
     6001 attcttcata accatttgga tggctgacat gccaatccca tgctgttttg ataatcgctt
     6061 tgatatctgt gtattcaggt ttccagccca gaatctctct cgctttttca cttgaagcga
     6121 ctaatcggct aggatctcca gctcttcttg gtgctatttc tgctggaatt tcttttcctg
     6181 tcacttctct agctgcttct agcatttcct tgactgagta tcctttgctg ctgcctaaat
     6241 tgaagaagtt gctttcattt ccttctttca agtattccaa agctaaaata tgtgcagcaa
     6301 tcaaatcttc tacttgaaca taatctctga tacatgttcc atctggtgta tcataatcgt
     6361 ctccataaac agccaaggct tttctttgac caagagctac ttgtaaaatg attgggacaa
     6421 gatgcgtttc tggtgtatga tcttctccga tagatgcatc tgcttttgct ccggctacat
     6481 tgaagtagcg caaagcgaca tagcgcatgc catatgcttg gtcacaccac ttcatcattt
     6541 tttccatcat caatttactt tcaccatacg gatttttagg attagctggt gtatcttctg
     6601 tgattggaga ctctttcggc tctccataag ttgctgcagt agaagagaat acgattttat
     6661 tcacgttgaa ctcgtgcatg acttcaagta agatttgcat accgtatacg ttgttgttga
     6721 aatacatcaa tggtttttcg acagattctc ctactaaaga gcttgcagca aaatggatta
     6781 ctccttcaat cggttccttt tcaaatacgg aacgtagaaa ttctttatcc cggatgtctc
     6841 cttcataaaa atgagcatct ggatgtactg cttgtttgtg acctgtgagc aaattatcta
     6901 ccacagctac ttgatacccc ttttgaacta gctggtctac agcgtgcgaa ccgatataac
     6961 cggctccgcc tagtactaaa attgtcataa aaaacactcc tttgattag
//
LOCUS       DAGCYL010000142         6881 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.144, whole genome shotgun sequence.
ACCESSION   DAGCYL010000142 DAGCYL010000000
VERSION     DAGCYL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.144"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            238..594
                     /locus_tag="MB216_002217"
     CDS             238..594
                     /locus_tag="MB216_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBT4527010.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            604..1503
                     /locus_tag="MB216_002218"
     CDS             604..1503
                     /locus_tag="MB216_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBT4527011.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2020..2751
                     /locus_tag="MB216_002219"
     CDS             2020..2751
                     /locus_tag="MB216_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBT4527012.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2896..4923
                     /locus_tag="MB216_002220"
     CDS             2896..4923
                     /locus_tag="MB216_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBT4527013.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            5031..6128
                     /locus_tag="MB216_002221"
     CDS             5031..6128
                     /locus_tag="MB216_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBT4527014.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            6435..>6881
                     /locus_tag="MB216_002222"
     CDS             6435..>6881
                     /locus_tag="MB216_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4527015.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT         2420 a         1104 c         1395 g         1962 t
ORIGIN      
        1 tatagtattt tttgttattt aagaaaggaa taaggttaaa caaataacag gttgcttttt
       61 gggtcttttt ccgttcaaga aactgaacga agaaaaggga aagttgtctc ttttgtgata
      121 agagaaatca taataaacga atgacttact ttccaataaa ttgagaacag tcagaaccag
      181 acaaattagt tgcatctgat gaataaagtg attattctat gacatgaggt gatacagatg
      241 aaattagaag tgacaaaaga agcacaagaa gtattgaaga ataaattaga agcagacgat
      301 caacttttat tagatatcga aaacggtgac ggtccatttg cagaaagcca agtcagttgc
      361 caattggata cggcatttcg attgatcatt gtcaaaaaag atacacaaac agatctaagt
      421 ctttattctg tagtggctga tacaccagtt ggaccaatca aaatcaaaga cagtgcaata
      481 ctttacatgg atgatccatc cacacttgct ttagaaccaa cttataatag tttacaatta
      541 aaaggaccaa gcggcttgag aaaaggcaac ttgcaagttg ttcgaaaaga ctagagggat
      601 aaaatgggaa aattggcgat catcagcgac ctacatgtgg acatcaataa attcggcgaa
      661 tcagagctga tgcagctttg gcaagttttg caaaagcaca agatcactcg gcttcatcta
      721 gccggagata cggctaataa agtagaccgt tgcctagctg ttgtcgaatt tttcgtcaat
      781 aaaggattat ctacgacatt tcatttcggc aatcacgagt tggcagatgt gcccaatgaa
      841 cagatgatgg accattttcc agacccacat tttttaaatg aacggtatat tgctttaaat
      901 gagcagaccg ttcttttagg gatgaatggc tggtacgact atcagttttc cgaattgaaa
      961 gattacgagg caatcgtacg aatgaagcgt ttgtattggt atgatcggtt catccagcgt
     1021 gggaaaacag atctcgaggt caatgcagaa atgcttgcag ctgcaaaaaa tttgctagac
     1081 cagctggaag caaaaaaact tgaggtgatt ttatccactc atttcgttcc taaacgggca
     1141 tttatcatgt atcaatctgc tccttatgag cgttggaaca aattaaatgc atttttagga
     1201 tcagaatcat tcggagaatt acttgaccgt tattcaaata tcaaacaagt agtttttggc
     1261 catacgcatc gtcgctttga agataaatcc atcggtcaca cgatttacag ctgcagacct
     1321 tttggttatt attatgaatg gcagctgacc cgagattttg tcttgaaaga acagttgcta
     1381 gacagctatg atcctatgaa attacgtgtc ctcttgcgtg cccatcaccc agcttttcag
     1441 gcctatcaaa cacaacattt gcaagaggaa tttgaacaag cgatgacaat cgttccgtat
     1501 tgagacagca ttagtaatgt gtaaaagaaa ataccttgta aaacataagg ataagtaaga
     1561 gcagtagatc catttatcgt ggatatgctg ctttttttgc ggaaaataat cagcgaatgt
     1621 ttatttgatc ggatgatttg taactcgcag agtactagat gcaagcgtgt attctttttt
     1681 tgaacgataa tcacaaaaaa aaatataata tacatttttt atttaattat aattaatatt
     1741 gattgaagtc taggcagtca agtcacattt tgatagatag gagggggact tacgaattat
     1801 tttaaaggct atgacagctg tatttctaga ttgtgtttgc atcaagttgc aataaaaact
     1861 agaaatcaaa tcgggctgat aatcatttat aactaatcga tacataggac caaatatagg
     1921 tctatgttct tttatttcat aagtatcgta aaacagaatt ttgctatgga ttaatccaat
     1981 cgactgatat gtttagtaaa taaagaaaga ggaatgtcaa tgatgaaaaa agaaatcgta
     2041 ttaacagcag caatgatgtt attcagcatg gtagcaagta cgacttttgt ttctgcaact
     2101 gaagtatacc caaaggaata taacacagaa ggaacgatta catttgaagc aggagatgag
     2161 ggtgtaactc ctcctgttga tccagagaat cctgatccca ataaacctgt cgatccatca
     2221 gatcctccaa gccctggaac aggaggggca ttatcgattg attatggatc aaaattcaaa
     2281 tttgggactc aaaaaatttc tactgcggat aaaacgtatt atgcagctgc tgatgtgatg
     2341 aacgatgggt ctagaaaacc gacatatgtc caagtgacag ataggagaag taccttatct
     2401 gggtggaaat taagtgtctc tcaaccagaa cagtttaaaa ctgccagtgg ggatgaacta
     2461 gttggagcac agctgaaatt tacgaaaggg caggctgttt ctttggttga tccaacatat
     2521 acgccgcaaa cggtgaattc tgaattaacg ttgacaccgg gaggaaacaa tacgttagca
     2581 attaatgcta aatcaggaac aggtgtgggg acttgggtct atcgatttgg tgcaaatgaa
     2641 aatgaaaatc aagatgcggt acagctttcg gtacccggta aatctgtcaa gttagcgcaa
     2701 caatattcca ctaaattagt ttggacatta gaagatacac ctaacaacta gtttactgaa
     2761 aagaactgtt aaaaataaat acgtagtcat attgtcttgc gaggtaatcg agcaaaaatt
     2821 gtcaaaggct aggctaaatt tgtttggata cgaatagttc atctaggaat ttaacgatca
     2881 ggaggaaaag atatattgaa aaagaggagg atcgtgctca ttttaagcag tctcttagtg
     2941 actaatatct ttccgatcat agggtactct ttggaattga atcagttgga gcatggaaat
     3001 agaagagacg aagaaagcaa actcacttcg acattcgaca gtttaataga atccaaccag
     3061 acacaagaaa gcagtgagga tttaagtatt tcgagtagtg gagagaccac tgacaacgta
     3121 gaagctgagg gttcggacaa acaaggacta gaagatgcac caatttctgc ggttgagctg
     3181 cctgacgatc ccaatattat ttctatcgat aaaatttttc aaacacctat cggtactagc
     3241 acgtccatat tggaaaatgg gaagttactg cagttgaacc cagcagaaag gtctcaaaga
     3301 ggagctattt ggtcaaataa gaagattagt ttactctcgg attttacatt taaatcttat
     3361 ttatacttag gaaatcaacg agatagagcg ggagatggca tgacctttac attgactggc
     3421 gatcctagaa tggctagtaa tcccagtcaa gttattggat cgccaggtat gggaattggc
     3481 tcatattcta ctcgttctgg ccaaccttac attgcgaatg cgctctcgat tgagtttgat
     3541 acgtataaaa atagtggttc ttctgatcga atggatcgag agataactgc tgataataga
     3601 gggcatggac atgtcgcttt tgtcacgcct aaaccaaata ataataatta ttctggagag
     3661 catactggcg tgacggtagc tcctacattt ctttctaatg gcacatggcg cttgttaata
     3721 attaaatggg acgcggagca aagaagtctt acttatgatt tggaaggtgt cggtcactct
     3781 tctcggatta tacctgatct cattaggcag tttggttcta cagaagtaaa ttgggggttt
     3841 acttcttcaa caggtacttt ttttcaagaa aatgctgtcg ctgtcacgcg acttccatcg
     3901 aatgtcacat caacagctga tgtaaaagtc aacgatagtg attatggggc catcgctgaa
     3961 gctggaggaa acgataagat aactatcaga aatacattga aggtcgaggg ggattttctt
     4021 gaatataaga aaatgcagac tagtatcgac ctcccacaag aattaactat tcccgagcat
     4081 atcttgatca atggaaatca aattgatgcc aaagatatct tgataaccgg aaatcgagtc
     4141 acgctaaatc tgacagatta ttttattgct ggagatacgt taataatcga attggagaca
     4201 actttgcagg aagtaattcc tgaaaaagta ctcactatgc attttgaata ccttgaaaac
     4261 gggaatttga ttgagcaatc gaatgaaata aacctaaaga tcgcaaagct gaaggagata
     4321 gatatccatg tttattataa agataaagac agtcagattg atattgctga aatgaaaaca
     4381 atcactggga aaataggaga gaagtataaa gaaactccta ttgatattga agggtatgtt
     4441 tttgactcgg attcagcgaa tacagaaggc atcttctccg aaaatacaga ggatatctat
     4501 ttttattaca gaaaaggcaa actttatctc tcaactgctc ctcaatatgt caattttgga
     4561 aaacataaaa tatccagtag accattaagt gaattcggac agttttcagg tgggttgaaa
     4621 attatagatg aacgggcttt tggaagttgg aaattacaat tgaaacaaga gaaagttctt
     4681 acaaatggag attttgagct tccagaagct ctttcctttg ttacctctga aaatacaacg
     4741 gttataggga catccgctgt tacgattttc gaaagtgatc aaaagggtga aagcgatttg
     4801 tcctctcttt tagattctac agctcatcgt ggaatcagaa tagatattcc tgtagaaaac
     4861 caaagaatcg gaacctttga ggggaaacta tcttggatac ttgcagatgt cccaggaaat
     4921 taacaggaag agaagaatat aacaaaatta ttgagtttca agaagggcac aggtaccata
     4981 ggctcttgtg tcccacagag acaagatttt gttatagtct tctcttacat atgattaaag
     5041 gagcgatttt cttgagacca aaacaacaat tatttataac actcctagtt attttagggg
     5101 cactttttaa caatatatca gtgtcagcta gtgcatttaa tttttcagtg acgccgataa
     5161 cttcagagaa tcaaatcgac aagagaaaaa cttacttcga tcttcaactt gatccggatc
     5221 aggaagtaga agtaaaagcg gaattgagaa atgatacaga aaaagaagtg aaaatcgata
     5281 tttctgtgaa tagcgcaacc accaattcaa atgtgatggt tgaatacgga aaaaacgaga
     5341 ttgaaaaaga catgagcctg atctttgatc tggtagatta tgtgagctat ccacagatag
     5401 tgacgctaaa acctaaaagt gtccaaaccg ttgcttttca tgtcaggatg ccgaatgagc
     5461 gatttgatgg tgtattggct ggtgggatca cattccaaga aatccagacg gaaaaggacc
     5521 aaacagaaac taaggatcaa agcttatcga ttgagaatga atacacctat attattgctt
     5581 tgttgatgca acagacactg aacgaagttg ctccgaacct tactcttcat gaagttaaac
     5641 cagatcaaat caatgcaaga aatgtcatct tagcaaacgt tcaaaatgat caaaaaacct
     5701 atatcaatca agtagttatt gaaacaaaaa ttacaaaaaa aggacattct gaagttttat
     5761 atcaagagga aaaagaagga ttgcagattg cgccgaatac aaatttttct tttccaactg
     5821 ctttaaatgg tcaaccgcta accccaggtg aatatcacct tacaatgact gttttaggaa
     5881 atgaaaatga aaatggaaaa ttttcgagaa aaaaaggaaa tactacgatc aactatacca
     5941 atcaatgggt atttgaaaaa gattttacaa tagacggaaa agtagccaag gaattgaaca
     6001 cgacggatgt tacgattaaa aaagacaata attggattta tatcttagta gcttcacttt
     6061 tcttattgtt tattttatta tgcatacttt tgtttgtttt gagaaaaaaa gaagagaaag
     6121 aaaagtaagg ttaagataac aaatagtaaa tttttattat tgtaggatgg ttgggctctt
     6181 tttataacaa tataaaatat accattttat taataaaaat gcatactagt aaatgatata
     6241 attctaataa caggtttgat cgcgagaaag gccaaataaa acgctctcca aaatgttaaa
     6301 aaaaagacag gacgatgtat attatacaaa caagaatcgc tgacaacagt gactcggatc
     6361 caggaaatca gttttatccc cagctagttg gtgaatgcat ggaaagtaat atacgtagaa
     6421 agagctgatg actaatgaat tatgagtttt ttttaagcaa agaagatcac agaagttatt
     6481 cattacttaa atatttagaa gcaagctcaa atttatcaga gtcaatatcg aatgttcaag
     6541 aagagttgtc gctatcgaca tttctcttga aaaaaacgat cgataagctg aaacatgatt
     6601 tacaaaagtt cgatctagag aaaaatttgc gagtaaccgt cagtgaattg gatatatgtc
     6661 tggaaattaa tggaaaatgt tctagcaaag ctctgttatc tcgttatata acggattctt
     6721 tatctatgaa aatggtattg gctttctttc aggaaaaata tatctcagta gaaaattttg
     6781 ctaaacagca tcacgtgagt tattctgtgg cttataaagt attacaaaga ctaaaaagaa
     6841 atctaaaaaa atatcaaatt ttttttgaaa agagaaagtt g
//
LOCUS       DAGCYL010000143         6855 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.145, whole genome shotgun sequence.
ACCESSION   DAGCYL010000143 DAGCYL010000000
VERSION     DAGCYL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.145"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            25..1179
                     /locus_tag="MB216_002223"
     CDS             25..1179
                     /locus_tag="MB216_002223"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBT4527016.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1201..1953)
                     /locus_tag="MB216_002224"
     CDS             complement(1201..1953)
                     /locus_tag="MB216_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4527017.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2317..4983)
                     /locus_tag="MB216_002225"
     CDS             complement(2317..4983)
                     /locus_tag="MB216_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBT4527018.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5324..6775
                     /gene="cls"
                     /locus_tag="MB216_002226"
     CDS             5324..6775
                     /gene="cls"
                     /locus_tag="MB216_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBT4527019.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2024 a         1413 c         1248 g         2170 t
ORIGIN      
        1 tatcataagt aaagaggtgt tgaaatgaaa atagggattg atcgtctttc cttttttatt
       61 cctaatttat atttagatat gacagagttg gcagaaagcc gtggggatga tcctgcaaaa
      121 taccatatag ggattggtca agaccaaatg gcagtcaatc gtgcaaatga agacatcatt
      181 acactaggag caaatgctgc cagcaaaatc gtaacagaaa aagaccgtga gctaatcgac
      241 atggtcatcg tcggaacaga atccgggatt gatcattcaa aagcgagtgc ggtaattatc
      301 caccatttac tgaaaatcca atcttttgct cgttcttttg aagtaaaaga ggcttgctac
      361 ggtggcaccg cagctttgca catggcgaaa gaatatgtca aaaatcatcc agaacgaaaa
      421 gtactagtca tagcaagtga tattgctcgt tacggcttgg caagcggtgg tgaagtgacg
      481 caaggtgtcg gtgctgttgc gatgatgatc actcaaaacc cgcgtatttt atcgattgaa
      541 gacgacagcg tatttctgac agaagatatc tatgatttct ggcgtccaga ttatagcgaa
      601 tttcctgttg ttgatggtcc tttatctaat tctacgtata tcgaatcatt ccaaaaagtt
      661 tggaatcgac ataaagaatt gtcgggtcga ggactcgaag attatcaggc gattgctttc
      721 catattccgt atactaagat gggaaaaaag gcattgcaaa gcgtattaga ccaaacagac
      781 gaagaaaatc aggaacgtct tatggctcgc tatgaagaaa gcatccgtta cagccgacga
      841 atcggtaatc tttacactgg ttcattatac ctggggctaa cttctctact ggaaaattcg
      901 aaatcactac agccaggaga tcgcatcggt ctattcagct acggttctgg tgcagtaagt
      961 gaatttttta caggttatct ggaagaaaac tatcaagaat atctcttcgc acaatctcat
     1021 caagagatgc tagattctcg aacacggatc actgttgacg aatatgaaac gattttcagt
     1081 gagacgcttc ctgaacacgg agaatgtgca gaatatactt cagatgtacc tttttcgatt
     1141 acaaagatcg aaaatgatat tcgttactac aaaatataat aaaatcataa aaagaaccct
     1201 tcacgagtcg tgaaaggttc tttttttatc tgcggaccaa aggagcagtc tataggtcag
     1261 cttagtcgta ctaaggattt ctcgtttttc actgaatagt ttgcgaatgt acctggtcac
     1321 aacaggatct tttgcgtacg cgtcatagta tagcttcatc aattcttcag taattgattc
     1381 ctgtgtaaaa taagtaaaat cttttttctt cactttgaat ccttcatcaa ttaagtatgt
     1441 ttcaaattga tccattacaa ctggcgatgt attaggatct tcttctatca ttcctagttg
     1501 cttttcgata ggataaaaaa catcatcgat tgattccact actctgatta tcacgcacca
     1561 tttgcttgac tttttcatca atttttctaa ttgaataaga tgtaccaatg ctggatttgc
     1621 tgctgtaaaa accatttcat atgtctcatt tgattggata aattcttcta acgactgttt
     1681 gataaaggaa acatttggca actcattttt ttctgcttct ttttttgcat tttctagcat
     1741 tcctgaagaa aaatcgatca aatcatagcg attaatttct tttgctaagg aaagaaatct
     1801 gccagatcct ccagcaacgt ctgctgctgt ttcagcaggc aggactttct ccgttttcag
     1861 ataatttttt acatcttcag caatagttac ttgcgattct tgctggattt cataatattc
     1921 ttcagcaaat tcatcccatt ctttttcatt catttgattt cctctaattt tttgttatat
     1981 gttttcactt ttcggaataa atcattctcc tcttttatct attctatctc ttttgatgaa
     2041 taataaaatg attggctttc cgtctatcat aaataaaaaa agggactgtg accaaagtcg
     2101 tgtcaaagtc cctctttccg aataaacagt gtccaatcag atgaccctca gtattagctc
     2161 aaaatgctga ttttacaact tcttccttaa acggtgttct cctgccaact cttcggtcac
     2221 aagtcacaat attcaacaaa catgagaagc tgtttgttga aattgcacct tgtgactcag
     2281 aattaaacgg caggctcaca acctcgttga ttcgttttat ttatttcctt gtactcgtac
     2341 aatgatcaat ttagtgattt ccatcaaaac aattgtcaca agtgcgattc ccatacatgt
     2401 aaagacttct gtcaatccaa atgctgatgg gatagagaag acttctttca gtcctgggat
     2461 cattgttaag cagtaaagcg cgctacatac aactacagct agtaagacac ttctgttaga
     2521 gaagaaccct gcttggatag ctgtttgtgt gtttgaacgt gctgggaatg tttgcaatgt
     2581 ccgacaccaa atcaatgttg aaaatgacat ggctacaccg aattcaggtg aatgctgcat
     2641 gccaagccat tgagcaaaga taacagcaat cccaatcagc acaccacgat acgttacaga
     2701 aatcaatgtt ttgtccgtaa agattcctga atttggatct cttggcgggc ggttcatcgt
     2761 agcaggttca gctttttctg tacctaatgc aattgctggc agtgaatcat tgactaaatt
     2821 gatgaacaac agttgtaatg ctgtaaatgg attggaccag tcagcaacta aggcaaagat
     2881 gatcgcaatg atcgcaccta aatttcctgc aaatagataa gaaatagctt tcttgatatt
     2941 atcaaacaca ttacgtccta cttctactgc gctgacaata gaagcaaagt tatcatccgt
     3001 caataccatc gctgctgcat cttttgccac atctgtaccc gttcccatag caattccgat
     3061 gtctgcttgt ttcaatgctg gtgcgtcatt tacaccatca cctgtcatag ctgagatatg
     3121 acctttattt tgccaagcac gaacgatacg aattttattc tcaggtgaaa cacgtgcata
     3181 aacagtgact ttttctaaaa cttcgtttag ttgatcttct gtcaattcat ctagttccgt
     3241 cccggtcaaa gccaggtttc cttcttcaaa aatcccgatt tgttttgcga tagcaacagc
     3301 tgttgttttg tggtcaccag taatcatgat tgttttgatt cctgcacttc ttgcttctgt
     3361 taccgcctga gtcacttcag cacgtggagg atcgatcata gcaagcaaac caacaaggat
     3421 caaatcgtct tcatcttcaa atgataaatc aaaattatca agtggacggt aagcaaaagc
     3481 tagtacacgt aatgcgcgtg ttgaaaaagc ttcattttgc tcttggaatt ttttcatcaa
     3541 ttcatccgtc atcggaacga cttcaccatt tagcaatact tttttactgc gttggaagac
     3601 gatatctggt ccgcctttaa caaacaagta atttttgtta tcaattgtgt gtccagtaga
     3661 cattaatttt cgatctgagt caaaaggaag ttctgcttgt cttgggtagt tgtttcggat
     3721 ttcctgataa ggctggttta atttttcact aaagtcgata aacgctactt ccgtagggtc
     3781 gcccaattta gtaccgtctt cactgatcga tgcatcattc gctaaaacac tgatttcgat
     3841 caagcgacgt tcgtcagctg tccattttga agggtcattt gtgaaatccc cagtatcgcc
     3901 attacctaaa aagtagtcaa caactgtcat tttgttttgt gtcaatgtac cagtcttatc
     3961 tgtacagatg atacttgtcg aaccaagtgt ttcaactgct ggaagcttcc gaataatggc
     4021 atgacgtttt gccattttat tcgttcccaa agataacacg attgttacga tagactgtaa
     4081 tgcttctgga atagcagcta cggctacggc tacggcaaac atcactgcgc tgacgatatc
     4141 tgcagtcata tctcctgtac cttctccaag ataaatacga aacagttgga tacctaaaat
     4201 caataaagac aatgccaaaa tagccaaact tagtttttta ctgaatttat ccagacctcg
     4261 ttgtaacgga gtgacttgct cagttgttga ttccaaaagg ccagcaactt gtccgatctc
     4321 cgtattattc ccagttgaag tcactaagaa tttaccgcgt ccgtaagtta cgatcgttcc
     4381 actgaatacc atatttgtac gatctccgat tggtaccgta cttgtgatat ccgttacttc
     4441 tttttctgca gaagtcgact ctcctgtcag cataccttca tctactttta atgatcccgc
     4501 atctagtaaa cgtccatcag caggtacata atcgcctgcg tctaacaaca cgatatctcc
     4561 taccacaagt tcgtttgccg gaatcgtttt ttccttacca tcacgaatca ctcgagcatc
     4621 gggcgctgaa aggtttttca acgcttctaa agaaccttct gctttttttg tttggacaac
     4681 actgattata gaattcaaca tcaatactgc aaaaataact aagaattcga tgtagtcacc
     4741 catgaccatc tgaatcagtg cgacaatcag caatacgata accattgcat ctttgaaagt
     4801 ttctaagaac aatttccaag ttggcttctt cggcttttct tttagctggt tcaagccatc
     4861 tctttctaat ttttccttcg cttgcgtggt gctcaatcca tcggttgagc tctgcgtttg
     4921 ctgcatcaac gagtcgatcg tttctttata gaccgcttgt tgtgcttgtt ccttgttttc
     4981 caaatttcat cccccatttt ttgatcttgg ccctagtcct gtctgaaaaa aaagagacct
     5041 aagacaaata cgcatttgtc ttaggtctca ccatttcata caataccaga aaaccatttg
     5101 gttaacttac tgttgatatt atcgcaacaa acgttgccgg ttactcccct aaggagatat
     5161 tcaactgtac taatatccta ccttattaac ctacaaaaag caactacttt agcccgattt
     5221 acctacattt tcatgaaagg agtgaaaagt atattcacct tggtaggctt ctttcttcta
     5281 tcatgctata ctaatgttat tgtatgtaag gaggaacatt tttgtggtat ctagtattat
     5341 aaccgccctt tatctattaa atgcacttat tgctttggtg gctattttga tcaaaccccg
     5401 agatgtagca gccatttggg catggctctt agtattgttt gcccttcctg gggtgggctt
     5461 tgtattatat ttatttttcg gacgtggatt aacggataag aaaaaattct atctccgaca
     5521 aagtgacttg aaagaattag aaaactttca gtcttttaga ggagatacca ttgaacatta
     5581 cgatcctgac atgggcgata aagacaaaca gcaatttgtt gacttcttct cttcattaaa
     5641 tcgtatgccg ctgacaagaa tgaattctgt cactcttctc acagacggac aagagaaatt
     5701 ggattcactg cttcaagatc taaaaaaagc caaacattcg atccatatcg aatattacgc
     5761 atttgtgaca gataatatcg gccagcaagt cttacatgtt ttagaagaaa aagccgcaga
     5821 aggcgtggaa gttcgaatat tatatgatgc atttggctct catggcacaa aagcaaaaga
     5881 tttcaatcgt ctaatcaaaa atggtggaca tgtccataca tttgttacct cacaaagggc
     5941 attacttcgt ttccgattga attaccatga tcaccgaaaa atcgttgtga tcgatggaaa
     6001 gattagttat accggtggtt tcaatattgc caatcaatat gtaaatacaa caaaaaaatt
     6061 cggctattgg cgcgatacgc atatacggat tttcggtgcc gcttcttctt tgctccagct
     6121 tcgcttctta acagactgga acgtctcggt acctgaagaa aaaaaggtcg gctatcattt
     6181 gaattatttc tttaaaaaag cagatcgaga tgaatctaag cttgctgata catccatcca
     6241 gcttgtttca agcggaccga ataacgaaag ggaacaaatc aagctttcat ttatcaaatt
     6301 gattacttct gctaaaaaac gtgtttggat acagacacct taccttgttc ctgatgaaag
     6361 tgtcattgct gctttaaaaa tcgcaactgc ctctggtgta gatgtgaaaa tcatgattcc
     6421 caacaaaccg gatcatcctt ttatttatcg agcaacacaa tattatgctc ggcagctgat
     6481 caaggaaaat gtacaaatcc ttgtctatga gaacggcttc ctccatgcaa aaacattgat
     6541 aatggatgat gaaatctgca tggtaggttc agcaaatcaa gatattcgta gctaccgatt
     6601 gaattttgaa acaagtgctg tcatttacga tcctgagttt ttagaagaac ttgctactca
     6661 gttcaaagaa gatgagacac attgttcatc catgacaact gaaacagtca aggaaatgtc
     6721 taactggcta ttattcaagc aacaaatttc tcgattattt tctccaatcc tataattttt
     6781 ttattcaagt agagaaaaat atactaagat taaagggact gtgacaaaaa tcttgtcaca
     6841 gtcccttctt ccgaa
//
LOCUS       DAGCYL010000144         6781 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.146, whole genome shotgun sequence.
ACCESSION   DAGCYL010000144 DAGCYL010000000
VERSION     DAGCYL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.146"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..520)
                     /locus_tag="MB216_002227"
     CDS             complement(<1..520)
                     /locus_tag="MB216_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4527020.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKREL"
     gene            complement(492..1322)
                     /locus_tag="MB216_002228"
     CDS             complement(492..1322)
                     /locus_tag="MB216_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4527021.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            complement(1360..2751)
                     /locus_tag="MB216_002229"
     CDS             complement(1360..2751)
                     /locus_tag="MB216_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBT4527022.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVTPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
     gene            3086..4045
                     /locus_tag="MB216_002230"
     CDS             3086..4045
                     /locus_tag="MB216_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HBT4527023.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(4131..5225)
                     /locus_tag="MB216_002231"
     CDS             complement(4131..5225)
                     /locus_tag="MB216_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBT4527024.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            5414..6175
                     /locus_tag="MB216_002232"
     CDS             5414..6175
                     /locus_tag="MB216_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4527025.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(6290..6730)
                     /gene="fabZ"
                     /locus_tag="MB216_002233"
     CDS             complement(6290..6730)
                     /gene="fabZ"
                     /locus_tag="MB216_002233"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBT4527026.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT         2183 a         1324 c         1076 g         2198 t
ORIGIN      
        1 ataattccct tttaaaaaat ttattccata cgtaaaagtc cactgcctta cttctaaaca
       61 tttgctcaat tgcttcttca cttgaacaac caatcttctt ttcgggaaca aataaatttg
      121 cattttctat attttttcct ttacttaatc caaaagcaat gatatcgggg tgattcctat
      181 ggtagtcttt caaaacatta ttaaaacaat caggtaaaaa ataatcgtca ctatctaaaa
      241 atcctaccaa atttccagtt gcttctttta tcccatagtt cctagcaact gatggaccag
      301 cgttttccaa gtgataacat gctactttac tctctagatt agcaatttcg tcaccaatgt
      361 cactactacc atctgtagat ccatcatcaa ttaacaagat ttcaatattg ggataacctt
      421 gcgttaagac gctgtctaca gctcttctta aagtattctt tgcattgtaa aaaggtatga
      481 ttatagaaag tttagtttgt ttttctaagt tttgattcat atacttttcc cctaatttta
      541 ttcggtaata aactaattac aagatgtccg caaataacta tcagaaaatc ttttagtgat
      601 cctagaccat ctttccatac gcgatatcgg cctttaattc ctggtaaaag atatttactt
      661 cctccacgtc tagcatacat gtcagcacct gctctagcat gtactagaat ctcttggata
      721 ttatatcctt tatatcccgc cttcaatagt cttacccaca aataataatc ttcaaatcca
      781 attaatgggc gatagtttcc cacttctaaa atagcatttt tatcaaacat gactgtcata
      841 tgattaaaag ggtttcttct tttcgaaaac acacggattt catcattata ctcaggtaca
      901 atccttcttc cgataacatt gctaagtgtg ccagtaaatt cttcaatatt actcccaaca
      961 atcgctattt ctggatattc ttggaagatc tctaattgtt tttccaatct gtgtggttcc
     1021 atgatgtcat ctgtatccat tctggccact aagcgatttt tagtatgttc taatccagtt
     1081 gctaaagcaa tccccaatcc ttgatttttt tctaatttta caactgtaaa caagtgagga
     1141 actttttgct ggaaataagt gacaactgcc tgaagcttct catttatagg gccatcataa
     1201 accaatacga tttctgccgg catcaccgtt tgctcaaaga tactagctaa tgcttccttt
     1261 aaatattccg gcttttcttt atcatacaaa gaaagcagta tacttatatt tttattttcc
     1321 aatgttctct cctacttctt tgctcaaaat ttttccaatc taacgatacg ttttttcttt
     1381 tggaaatgca ctttctgcta atgaactgat attttcatct tcatctagtc cttgtgagga
     1441 catcttatca aataagattt tgattgtttg aagtaaaatc ttcaaatcta atagtaagga
     1501 atactttttg atatacaaca aatcaaaacg tagtttactg ttataatctg atacatattt
     1561 tccgtatact tgtgcatatc cggtaattcc cgcacgtaca ttgtgtctta agtaataata
     1621 aggattttct tcattgaact gatcaacgaa aaatggtctt tctggccttg gaccgacaat
     1681 cgacatatcg cctttcaata cattaaagat ttgaggtaat tcgtcaaaac gtacagcacg
     1741 gataaattta cctactggtg tcacacgtgc atcgttactt ttagccaaga ctggtcctga
     1801 ttttgcttca gctgtcgcac tcatggaacg gaatttatag attgaaaatt ccttttgatt
     1861 tttcgtaata cgtacttgtt tatagatgat tggtccaggt gaagttgttt ttaccaaaat
     1921 cgctgtgatt agcataaaag gtgaggcgag gatcagtaaa ataagcgaaa cgatgatatc
     1981 aaagagacgc ttgaatagtt cgtattctgg ttggattctg aacccagaag cttcaatgat
     2041 gctttcatct tcaaaattca taatattcgg atttatcatt gtcagattct caaaagtcgt
     2101 atttaaaaat aatttcttct cttttttagt cactaattga taaatgttga ttttttcatc
     2161 ttctgggatt ttagaagcca aatagacgat atccatatca tcgatcaatt tttttgtatt
     2221 ttcgtaataa ttatcgattg ccactgcttc aaccttatgt ttattgtttt tcttagattg
     2281 aaaattttgg atagctggtg tgacatcttt ctccaaaccc acgataagga ctttcttatc
     2341 acgactgact cttaagtaca gtttgtaaac gactatccgg taaacactaa gtaatatcgt
     2401 acctaggata aacgagatca aaatgacgcc acgcggaaat gcaaaccatc ggccagcaaa
     2461 agtaactacc atgatcccca atgctattag tacttgtcca ataacagtaa tgaagacaat
     2521 atcactgatc attcggttat agaatacata agctcctaac aaaatattca acgcaataaa
     2581 gatgatggaa ataaaaattg ctgaatgctg aaaggtttct aagtttctca ctggtacttc
     2641 tccctggaat ttcagcaaaa aactaagata gatcgataca tttaatacaa gtacatccat
     2701 aagaataatg aatattctgc gtgccgcatt ccactcccca tttttattca tatatacact
     2761 cctaaccata aaataatgcc tctatcatct taccatagca aagtttaaaa acctagtgaa
     2821 taaccaaatt gtaaaggaaa ttaaaagaaa cggggaaaag cgtttattgt ctgtttatat
     2881 gtgtatttct gcataacaga aagttgtctc agaagtccca tcacttcctt tatgctgaac
     2941 tagtattctc ctgctaattc cttattcaca agttacaata ttcatataac gcgaattgta
     3001 aaattaagtt agaaaaataa aaaggcattt atggtacact caaattgtat ttccgacgaa
     3061 agaaaacaaa ggagtgtaac cataaatgac ctacaaacat cttaccatag acgaactgac
     3121 aatgatagaa tcatattatc ttcaacataa taagccggtt gaaatcgcta accgaatggg
     3181 acgtgctata caaactattt ataatgtagt caataagttc aagcaaggca agactgctct
     3241 tgattattgg caccagtata aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt
     3301 acctgctcat gaagtagatt acattaaaga gaaagtcact cttggttgga cgcctgacgt
     3361 cattatcggg cgaaaagaaa ggtctgtttc atgcggtatg agaacacttt atcgtttatt
     3421 ttctaaagga atatttgata ttgacacact accgatgaaa ggtaaaagaa aacccaatgg
     3481 ccatcaggaa aaacggggaa aacaacagta tcagcgctca atccatgatc gacctgataa
     3541 ttatcctgat ttcaattctg agtttggtca ccttgaaggt gatacgatcg ttggcattca
     3601 tcataaaagt gccgtcatta ctttagttga aagattatct aaagtcatta tcacgattaa
     3661 acccaacggc cgtaaggcat tagatattga aactgccctt aatcaatggt tttctcgctt
     3721 ccctaaaaac ttctttaaat ctattacgtt tgactgtgga aaagaatttt ctaactggaa
     3781 agccattagt aaccaacatg atattgatat atattttgcg gaccctggaa caccttctca
     3841 acgcccatta aacgagaatt ctaacgggat tctgcgtcgt aatggactgc cgaaatcaat
     3901 ggattttaga aaagtgaatc agacatttat ttccagtgtc agcaatcaac gtaatcatat
     3961 tccaagaaaa tcattgaatt acagaacacc aatcgaaata tttttgagct atgtacaaga
     4021 agcattttat tctagcttaa tttgacaaat cataacctat aaaaaaacaa aataaaaaag
     4081 cagaaataac tggctatttc tagcggatta tttctgcaat aactaattat ctatttttta
     4141 ttttcaatta atttaaagct tgaccatggt ttcaaaaagt caaggaggaa taattcgtca
     4201 tcggagattc ttcctacaac attgacacgt ccgtcgtttt tcatatcctt caaggcgatt
     4261 tgtgtttctc ctttgtattg accgtaccct tcattgtcaa tcagtacatc cccatgatgc
     4321 atatctcgcg tattatgagg cgggaaatct ttatctttgt agtatacacg agtcatcgtt
     4381 gaacgaagga tatattctga gcgatcccct cgataactat gaaggttatc aaacaagcag
     4441 attcgttcgt tttctgtaat accttcttcc gtatctactt tcaatgttgg ataatccgcg
     4501 ttaaatgctt ctgccatttc tttcaattct tcttcacttg cgtacgcatt tccaatagaa
     4561 atatcatcaa tcaatcctgt aaggattaaa tgcttcactt gtgtcgcaat ttcaagatct
     4621 cggtggtctt ccagcgtgca caagccatct tgcgtaggcc aagggccaaa ctcagcagat
     4681 tgcgaattga cgaatgccat cgtattcaaa ttatattttc tgaatttttc tgaacaaaaa
     4741 acaaagtgtt cgtatcctaa accagagtat cgatgaggat aaaaattatg agaacctaat
     4801 aaattatccg tgtttggcga ataactcatg atgttgtcaa cgtaattcgt gcccgaactc
     4861 atattgatct cgattttgat accaaaagga ttgcgcgtca tttttgcttc ttctgctcct
     4921 gtgaatccga tatctaagcg gaccccgtag gcacccatct tatggaaaaa agataagtca
     4981 tcgtaagaaa tctccaactg ttcaaataac gctggattga tatcaaccat tacttccatg
     5041 cctaggctat tcgcgtaatc aactacttct ttaaattctg aaagaacttt ttctttatca
     5101 tcattgatct gcagcaaact ggtaaaaacc cgtttatacc catacttatg agctaaatcc
     5161 aagtacgctt tgtccttttc aaatgttgac cgttctggat aaatcgagat tcctaatttt
     5221 cccatattac catttcctcc tgcatctttt cgtttttgta tccacttaca ttatagcaaa
     5281 cacaaagtaa atggtctaca ccttttcttt ttcttttaaa ataaatgata aattccaata
     5341 atttaagtag gtattttccc tctattttgt tatcattaaa gataacaaaa tagtaaattg
     5401 gaaaggaaca gttatgaaga aaaataaagt tattttaagt attagtttag cagcagcatt
     5461 gctgtttgct gctggttgtt ctgcaaacaa cacgacttct tctagttcgt ctacagaaag
     5521 ttcaaaaagt acatctactg cggaaagcac gactgccagt agcgtcgatt tgaacgcatt
     5581 ggaattacca caattgacga aagatgtcgc cgaaaatgaa gatctggtcg aaatggtgac
     5641 gaataaaggc acaatcaaaa tcaaattatt tcccgaacac gcaccaaaag cggttgaaaa
     5701 ctttatgaca catgcaaaag acggttacta caacggactg actttccatc gcgtcatcaa
     5761 agatttcatg atccaaggtg gcgatcctaa aggcgatggc actggagggg aaagcatttg
     5821 gggagaagga tttgaaacag agatcaacaa ccacctttat aatataagag gtgcgttatc
     5881 tatggctcgc tcacaagatc ctaacagtaa cggcagccag ttctttatcg ttcaaaatac
     5941 agatgatatg catgatggtc ttttaaaaga cgactatcct caagcaatca tcgacgctta
     6001 taaaaacggc ggttatcctt cactagatgg ccattatact gtcttcggcc aagtgattga
     6061 aggaatggat gtcgtggatg ctattgcaaa tatcgaaaca gatagttcag acaaaccaaa
     6121 agaagaagta aaaatcgata aaatcaatat actgcaagaa gcaaaacaaa aataaaaaaa
     6181 taacgtctga atcctgcata gatgacaacg tatgctagga ttcaggcgtc ttctttttac
     6241 ataaaaagca agtaagcttc cttttgtttt gctatatcag gttttatagt caagcatttt
     6301 ggttgacgat gaacgtgaac tcgcactcac agactttttt atcatctaca tatgctgcag
     6361 ataacgctgt accgactgat cccttcattt tggtgatttc aaaatgacat ttcagcacat
     6421 ctcctggaac aactttttga cggaatttgg ccttattgat cccaccgata taagctcttt
     6481 ttcctagaaa ctcttctgat tttaggataa gtatcgaacc tgcttgtgcc agtgtttcta
     6541 gaatcaacgc acccggcatc actggattgt caggaaagtg accttggaaa aaatcttcat
     6601 tgattgttac attttttgtc gcaacgatct tttttcctgg ttctaaagta tctacataat
     6661 caatataaca aataggataa cgattaggaa tcaattccat tacttcagta gcagataata
     6721 cgtaagacat aattaaactc cttctttcag gcaattgcca ttttcattac tttgactatc
     6781 a
//
LOCUS       DAGCYL010000145         6575 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.147, whole genome shotgun sequence.
ACCESSION   DAGCYL010000145 DAGCYL010000000
VERSION     DAGCYL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.147"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(663..1142)
                     /locus_tag="MB216_002234"
                     /pseudo
     CDS             complement(663..1142)
                     /locus_tag="MB216_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(1301..2002)
                     /locus_tag="MB216_002235"
     CDS             complement(1301..2002)
                     /locus_tag="MB216_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4527027.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(2110..3519)
                     /locus_tag="MB216_002236"
     CDS             complement(2110..3519)
                     /locus_tag="MB216_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBT4527028.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(3567..5432)
                     /locus_tag="MB216_002237"
     CDS             complement(3567..5432)
                     /locus_tag="MB216_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4527029.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(5553..6404)
                     /locus_tag="MB216_002238"
     CDS             complement(5553..6404)
                     /locus_tag="MB216_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4527030.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT         1879 a         1474 c         1100 g         2122 t
ORIGIN      
        1 tatgaaatga cttacttata gaagatactg ggatttcgtc ctatatgcca ataacgtgta
       61 ttattttaaa caacaaatac ttgtttcttt tattaacgaa aaaatcattt tgttctcgtg
      121 ttatgtttca ctgataaatg atgtttctct aaataaaaaa gccgacgaac taaacgatca
      181 aaatttgatc atttttgttc atcagcttct cctaaaaatc tttttgttaa cgataaatag
      241 ctggatttgt aaaaatatca cttgaatcgt cactgctgga agaaaattca gtgataaccg
      301 cacctgttgc gcctgcctgg aactagtgtt tgatattggg ctcgaacgtg tactgatctc
      361 tagcatgaag aataatttct ttttttactg tataataact ttctttcctt tttggaatag
      421 tacaggaaag tttttttgtt tcctttcctt cgacataagg tataccagac cctttcgaca
      481 acgaaaggtt ctcatttttc cttcatcgtc atttcaagga gggtgccgat gctctggaca
      541 gtttcgatcg ctcctaaacc aaaatctgta tattcgattc tttctaactc tgattctttc
      601 ataccattcc tgtctcaagg tacatctatt gtactttttt cttgtaatcc attctgcctc
      661 tcctaactaa ttgtcaaatc atcccaagat atgtcttctt cctctttttc ttcttggatg
      721 atttctgcat tcggatctac tggttttttc aataataaaa tggttattcc tgtgacaaaa
      781 gaaccagcta acaatgcacc aatgaatgcc catggtctgg tcatcgtagc gactgcaaac
      841 attcccgatg ccggaatacg gctatctgcg ccccaaaaca tgcttaatcc gccacctact
      901 gtcccaccga ctcccgttgc tatgacattg cgtgccaagt cgttcaatac gatagggaaa
      961 catccctctg tgatctgaca gatccccata ggaaaagcgg tcttcaatgt atcgatttct
     1021 tgacgattga gtatattttt acgaatgatt ttccctaaga aataagcgat cgtcaagccg
     1081 aatggggtta ccatcgaagc taaaatcaat acagtgattg gttcatagat tccttctgaa
     1141 aatcgtgcca caccccctta tccagaataa atggtaatcc agaaacagcc gtgtatctgc
     1201 ggtaataagc ctaaccaaac gaaaaatatg agaagctatt tttgtttggc tgttcttatt
     1261 acttgatgca gggcagcttt ttcacaacct ctttttttat ttatgtttca taatcaatat
     1321 ggtaaggatg cagctccgta tggtagaccg cctccgaata ttcaatcatt tccccaattt
     1381 gcgtagacga tttccgtatc cgtttgatgc cgatctgtgc gtctgttttc agaattcctt
     1441 gttcttcttc tgttaaaaat actgccgaga aatcatcaga aaatgagtca atagtatatc
     1501 cttttttcgt cagcaaacga tacaaagatt ctttgccaaa ttcttctctg ctgaattgca
     1561 tcatttcttg tggaagataa tgaacaaaat aaatataagg ctgattgttc aaataatata
     1621 agcgcttaaa acaaacagct tgctcaccga aaaaggaaaa caaaggatct tccttcttta
     1681 tatggaccaa ttgcaaatcc agcgtttctt tccggacttc atatcgggag ttctccaaga
     1741 tttgcgtaaa gcttgccgct ttggataatc gattgtaagg acgattgctt agaaccgtcg
     1801 tcccacgacc acttttcttt gcgacatatt catctgatgc cagcagttcg attgcctttc
     1861 ggacagtgat tttgctgact tgaaacagtt cttctaattc actttctgtc ggcaacatcg
     1921 aaccgactgg atattttcct gagaaaatat cttctttaat tttatctgcg acttccaagt
     1981 atagtacacg tgtccttttc ataacgtcac cctttgcttt actattacta aaagaatagc
     2041 acataaaaaa taaaaaaaga acacaaatca catgtgagaa gtgtgttctt tttcattcag
     2101 ctgctttaat tacaagttta cgccatttga ggcaattact tctttatacc aatcaaaaga
     2161 ttttttcttt gatcgattca gtgtcccatt tccttcatcg tctagatcca catagataaa
     2221 gccgtaacgt ttactcattt ctcctgttga tgcactgaca agatcgatac atccccaagg
     2281 cgtatagccc atcaaatcta caccgtctgc tactgcatta gccatcgctt cgatatgacg
     2341 acgtaaataa tcgatccgat aatcatcaat aatcgtgcca tcctcttcca tatgatcaat
     2401 ggctccaaga ccgttttcaa caatgaataa tggtttctgg taacgattgt ataattcatt
     2461 caatgcgatt ctcagccctt ctggatcgat ctgccagccc cattcacttg cctcaagaaa
     2521 cggatttttt actcctccca gcaagttccc ttctgaagtt gctgcttgtg gatcagtctc
     2581 attgattgcc gtagacatat aataactaaa tccgatgtag tccactggat attcttttag
     2641 caactggaag tctttttccg tttgctctaa tttttccgga tcgactccat attttttatg
     2701 ggtacgcgct gtgtatgcag ggtattctcc acgtacttgg acatccgtgc agaaaaaatt
     2761 gaattcttgg ttttggatca ttgttgctgc ctgattgact ggattggcat cgatgctgta
     2821 agttgttgca tagataatca tgcagccgat ttggatatcc gaacgcaatt cgtggccgat
     2881 ttttacggct ttgctacttg caacgaattg attatgccat gcttggaaaa tattttggta
     2941 ctctcctgct ccattgcttt ttactaatcc ttgacttaaa gctggaaagt gaaaggccga
     3001 attgatttca ttgaaagtca tccaatattt gactttactg ctgtatctct ctaatactgt
     3061 ctgagcaaat cgttcataga agtcgatcag ttttcgattt ttccagccac cgtattcttt
     3121 tgccaaatga agaggcattt cgtaatgaga aatcgtgatc acaggttcaa tatcatattt
     3181 taagcactca tcaatcagct gatcataaaa ttctaatccc gcttcgttag gagtaccttc
     3241 gtctccattt ggaaaaattc gtgtccaggc aatcgaaaaa cgataacatt tgaatcccat
     3301 ttccgcaaac aaggcaagat cttctttgaa acgatcgtaa tgatcgattc ctcgatgatt
     3361 cgggtaaata tatttttctt gatcaatcgt ccagtcaaat tcttcactgc cgataatcgc
     3421 aaagcgctgc ttacctccag gcatagcatc tgcaacggat agtccttttc cgtctcgatc
     3481 ataagcccct tctaattgat ttggcagcag ttgctccacc ccataaaaat ttttctgaaa
     3541 aacgtgattg catcctgatt tcctccttat tttacaattg ccagaacatc ttcgtttgca
     3601 ataatctctt tttggtctaa ttcaagtata tcggcaaatg catcagtatt cgtaatgaca
     3661 actggagtga ttacgctata gccagctgct ctaattgctt cgatgtcaaa acgaatcaat
     3721 aaatctcctg ctttgacgag atccccttgt ttgactaaga tctcaaagcc attcccattt
     3781 aattctactg tatccatccc gatatgaatc aaaatctccg ctccgtcagt cgttgtgatc
     3841 cctacagcat gtccagttgg aaaaatagtc gtgatctctc cgtctgcagg tgcaactagt
     3901 tctcctactt ctggatcaat agcgatacct tttcccatca cgccagaaga aaaaacttgg
     3961 tctggtactt cactcaatgg cagtacttta cccgttaaag gactttttaa ggtatgccgt
     4021 gctgaagtaa tcggttctcc tgcattcgca tgtttgtttt ctaattgatt gtcttgtgtt
     4081 tgttcgtcaa atcctaaaat caatgtacct acgaatgcaa gaacaaaagc aattcctgta
     4141 gctattactg ctactgtcac atttgactct acaccatcca ccgtattaat gaaagtcgga
     4201 atcgtcaata agctgaccaa actgctggag aaagctttta ctccggaaaa tccgataata
     4261 gctccaccaa tacctccaga aatacaagcg gcaatgaatg gtttcttcaa aggtaatgtc
     4321 acaccataca cagttggttc agtgattcca aataacgaag tcactgtaga agaaatccct
     4381 aatgctcgaa gtttcgtatc ttttgttcgc aatgctacag ctaaagcagc cccaccttgt
     4441 gccaagatgg ctggaagaag cattggcatc aatacatcaa acccatattg ttcgatattc
     4501 aaaaacatga tcggtacgaa tccccaatgc atgccgaaca tcacgaatac ttgccataaa
     4561 cttcccatga ttagacctgc tacgattgga ctaaatccat aaatcgagtt gaagagaccg
     4621 cctaaagcat taccggcaac cgtcccgatt ggtcctaaag caagaaatgt taatggaacc
     4681 atcactacta aaacgacaag cggaactaaa atcatttgca gaaattgcgg aatcactttt
     4741 tttacgaatg gctctaattt actttgaacc catacggcta gaatgattgg tatgacagaa
     4801 gaagtataac cagatggtga aagaatcact gggatcccaa agaaactgat agcttctccc
     4861 gcaccagcaa ctgctgtgat agctggatac acaagtgcca tagcaattgc tactgctaaa
     4921 aactggttgg ctttgaactt tttagctgct gtaaaagcaa gcatgacagg taagaaggta
     4981 aagacaccat ctgctgcagc aaacaatatt tgataagtac cgctatcagc tgtcagccag
     5041 cccatcaccg ttgccaatga taagaatcct ttcaataccc cggcggctgc catcgcccct
     5101 agaaaaggtg taaatatact agagataata tcgatcagac gattcaaaat atttttggac
     5161 tcttcttttc catcctccga aactgcaaga ccttcctcat ccacgatcga ttgataaaca
     5221 tctgctacat tactgccaat cactacttga tactgaccgc cactttgtac gacttggatc
     5281 acgtccgggt cttcttttaa cttttgcgta tctgctaaag attcatcttt taatctgaat
     5341 cgcaaccgcg tagcacaatg tactaaactt ttgacattct tttgtcctcc taccgcttcc
     5401 catacacgac gaccaactgc ttgattatcc attgtttttt ccttctttct tttttattta
     5461 aaaaataaaa acccagattg aaaaaaacag tcacaaacat tcgtgaacct attttttcaa
     5521 tccaggtttt gcctgccata cagtaacaat ccttatgatt ggatgctttt tttctcactt
     5581 actactcttt gaatatgaat cgttaaataa atctgctctg cgatccccat ttcctgtttt
     5641 tgcgtttgtc gaagatactc attgatccgt tcgacacaat gaaacgcttt tggatacttt
     5701 ccttgaacta atgcgtagag aaattcatct tgctcgtcag atccttcccc tttgagataa
     5761 cgctgggcaa aaaactgcag atgagtaatg atacgctgat aattcaaaga ttcttcatcg
     5821 aacacgatgc cgaagaattt actgatgata ctcagtatat ctcttaccat tgtggtagcg
     5881 gtcatcgtca cttgcatgtc ttcgtttcct tgttggctat tcacaaaatg aaaggcaata
     5941 aaaccggctt catcctcaga aagttccaca cccgtctttt gtttcacgaa caaaagagcc
     6001 tctttcgctg cttgatattc tttcggataa aatttttttg tttcaaataa taatggatta
     6061 ggcagttgga tattttcttt tgctcgtgtg atcgcataat gaagatgatc ggtcaatgtc
     6121 agataaatat taggcgaaaa ttcaactttt agtttatctt cagccagttc gactacttgt
     6181 ttgacccatt cgatctcctc tgccggaata tcagccatca gctgctggaa ttggttttta
     6241 gcaaaagaat ctcttaaaac aaattctttt tggaccgctt ctggcgaaat ctcgtcccca
     6301 atcttttttt gaaaagctag tccttttccc atacaaacga cttcttcccc gaatccattt
     6361 ttcgaaatga cgacattgtt attcaaaatt ttatcaattt tcattttgct tcgccctctc
     6421 aagaaaaata aaaaaaccta gaattcgcct actgtttctt agctgaattc tggttttgcc
     6481 tgccgtacag taacaatcca tttcttattc aaaatcatca taccatcgaa tttttattta
     6541 tgcaagcctt ttcatataaa aaataaaaaa actgg
//
LOCUS       DAGCYL010000146         6536 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.148, whole genome shotgun sequence.
ACCESSION   DAGCYL010000146 DAGCYL010000000
VERSION     DAGCYL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.148"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            252..2018
                     /locus_tag="MB216_002239"
     CDS             252..2018
                     /locus_tag="MB216_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBT4527031.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2120..3379
                     /locus_tag="MB216_002240"
     CDS             2120..3379
                     /locus_tag="MB216_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBT4527032.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3526..4827
                     /locus_tag="MB216_002241"
     CDS             3526..4827
                     /locus_tag="MB216_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4527033.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4830..5681
                     /locus_tag="MB216_002242"
     CDS             4830..5681
                     /locus_tag="MB216_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4527034.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5694..6509
                     /locus_tag="MB216_002243"
     CDS             5694..6509
                     /locus_tag="MB216_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBT4527035.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
BASE COUNT         2087 a         1144 c         1325 g         1980 t
ORIGIN      
        1 ccaaaaactg ttaaaaaact tttactttag aaaattggag aaattgatcg cttaagataa
       61 attcaggaca ctaaatagta caggaagtag actaaaactt ttaaaagttt taagtctatc
      121 tatttggcaa gtttaaaaaa taaaaattcg gtatcaaaaa actgttaccg ataacatttt
      181 tgaaagcgtt gtcttttgct tctctatcac ttatagtgat gacgtcatat taaaacacaa
      241 ggaggaaatc aatggataca gctgcaattt atcatcgacc ggaaagtgaa tatgcatttc
      301 tatatacgaa tacaagattt cgtattcggc ttcgtactag gaaaaatgat atcaaaaaag
      361 tatatgtgct atgtggagat ccatatacca taacaactga aaaatggtat caaaaacaac
      421 gaccaatgaa aaaatgtcta agtaccaatg ttcatgatta ttgggaaatt gaagtgacga
      481 gcgaaacaag tcgcttacag tatgcgttcc atgttgttgg ggaagataac acggactgtt
      541 tttacggtga tcaaggaatt tttccttatc gagaaaatgt gattactgaa ccaaattttt
      601 attttcgaat accttatttt catcaaatcg atcgattcac tattccagaa tgggtaaaag
      661 aaacagtatg gtatcaaatt tttcccgaaa gatttgcaaa cggggacaaa gcaaatgacc
      721 ccgaacacac tttaccttgg ggaagtaaag atccagaccg agaagacttt ttcgggggag
      781 atttgcaagg tgtgatggat catttggatt acttgacgga ccttggcatc aatggcatct
      841 atttttgtcc catatttaaa gccacatcca atcataaata cgatacgatt gattattatg
      901 aggtggatcc cgcttttggc gataaaaaac ttttaaaaaa cttgattgat gaagcacata
      961 aaagagggat acgtatcatg ttcgatgctg tattcaatca tatgggtgtc cattcaccac
     1021 agtggcaaga cgttctcaaa aacggagaaa aatcgatcta taaagactgg ttccacatcc
     1081 gttttttccc agtagattct tatcagatga ctgacttacc agaaactgct gaaaatatcc
     1141 catatgatac ttttgctttt actccattca tgccgaaact aaatacagca aatccagaag
     1201 tgcaaaaata tttgctggat atcgcaactt attggataaa agaattcgat attgatggat
     1261 ggcgtcttga tgttgctaat gaagtcgatc atcatttttg gaaaaaattc agagaagcag
     1321 ttacagaaat caaaccagac atctatatcc ttggagagat ttggcattcc tctcaagctt
     1381 ggctgcaagg ggatgaattc catgcagtga tgaattatgc atttacagat tcgattaaag
     1441 attattttgc aaaaaagaaa atcacagctt cacaaatggt cagtggaatg aatcatcagc
     1501 aaatgcttta cagagatcag gtgaacgaag gaacattcaa cttattagat tctcatgata
     1561 ctgccaggat cttgaccctt tgccaaggta acaaagaatt gatgaagtct gtgatggcct
     1621 ttatgtttct tcaaaaaggg tctccttgta tctattacgg gacagaaata gggatgactg
     1681 gtgaagatga cccggattgc cgcaaatgta tgatatggga gaaggaggaa caagatctag
     1741 agctattcgg ttttataaaa gaacttgttt ctctaaggaa acagttgtct aagattattt
     1801 ctgaaggttc tactcagtgg ctaatagtaa atgatcgtga agataagctc tatttcacac
     1861 ggaaactaga gggacagatc atctatgttt actttaatca gtcaaaagaa ccttgggtag
     1921 tagaacagga aaatgaggtg attcttagtc aaaattgtca gttgttagaa gacgggaaag
     1981 cagagatacg acaatacgga tttttgatca tgtgttgaaa actgttaccg gtaacaacaa
     2041 gaaagcgttt gcaaatgaag atttgtgccc attataataa gggtgtaaca gcacgttgaa
     2101 tacaaaggag gaaatgaaga tgaaaaaaag aagtgtgaaa cagttgggtt tagggttaat
     2161 ctctttagga atcttaggcg gattagcggc ttgtggcaat ggtaactctt cagcagaagg
     2221 ggaagccgat gaaggaaaaa cgttaacagt ttccgttgat tctggatata aagattatat
     2281 aaacgaaata aaaaacactt tcgaaaaaga aaacgatgtg aaaatcaagc tagtagaaaa
     2341 ggacatgttc gatcagttgg aatcattagc gttagatggg cctgctggaa agggacctga
     2401 cgttatgatg gcagcatatg accggatcgg tgcgttagga cagcaaggac atctggcgga
     2461 agtaaaactg ggaaatgaag cagcttacga tgaaacagac aaagcccaag tgacttatga
     2521 tggaaaaatc tacggagaac ctgcagtaat cgagaccctt gttttgtact ataacaaaga
     2581 tttagtcgat acagcaccat caacgtttaa agatttggag aatctatcca aagacagtcg
     2641 ttttgctttt gagtcagaag caggaaaaaa tacaggattt ttagctaaat ggactgactt
     2701 ttattattcc tatggtttaa ttgcaggtta cggtggctat gtatttggtg atgatggcac
     2761 aaatccttca gatatcggtt tgaataatgc aggtgctgta gaaggaatct cttatgcaac
     2821 agattggttc aaaaatgttt ggccaaaggg aatgcaagac ataaaaagtg caggagactt
     2881 cgccagccag caattcatgt ccaataaaac tgcagcaatc attgacggtc catggcaagc
     2941 gcaaacatac aaagaaaaca atatcaatta tggtgtagcc aaaattccta ctctaaataa
     3001 tggacagcct tatcaaccgt ttggcggagg aaaaggatgg gtagtcagca attatgctaa
     3061 aaacaaagac ctctctcaaa aatggcttga ttatgtaacg aatcaggaaa atcaagaaaa
     3121 attctttgaa atggtaaatg aaatccctgc aaaccaacaa gctagggaaa cagcaaaggc
     3181 taaaaacgat gaattaacaa cagctgtgat tgaacaatat gaaacagctc aagcgatgcc
     3241 aaacatccca gaaatgggcg aagtttggac aggtgcagaa aacttgatgt tcgatgcagc
     3301 atcaggttca aaaacaccaa aagaatccgc agatgaagca gtaaaaacaa tcagtgaagc
     3361 catagaacaa aaatactgat taaaatgata accgtccatt tttataaaaa atggaacagg
     3421 tgcttcagat aaaagtggca agagcgagat ggaaaattat cttgctcttg ttgccattat
     3481 cttaaaagca gtttgcggtt tagcaagttt agagggggca ttcagatgca gcaagataaa
     3541 acaagcaata tcaacgaaaa aaaaatcacg ctttgttcac ttattccagg gatggggcag
     3601 tttgtaaatg gccaagcttt taaaggtatc gtttttttcg ccatattcgt cctattcatt
     3661 ggtcagatgc ttctgggcgg atttcatgca ttggtaggat tggttacttt gggaagtata
     3721 ccaatggaag atcattcatt atttattttg attgaaggca ctttgcaatt catcattaca
     3781 atcattttct tgatttttta cggtttgaat cttttagacg cttatcgagt agcgaagatg
     3841 cgtcaagagg gcaaaaaaat caatcggacg atgaaggaag tcatacataa tataggtgat
     3901 gaaggttttc cttatctatt gacacttccc gcttatcttc tcatgatttt tacgatcatt
     3961 ttcccagtcc tagttacatt gttcactgca tttactaatt atgacttcaa tcatattcca
     4021 ccagccagtt taattgattg ggtaggggct aaaaactttt ttagtatctt ctttttaagc
     4081 tcatatcgta atactttttt tgcagtcttt tcgtggaccg tgatttggac tttatgtgca
     4141 acaactttgc aaatcaccct tggtattctc acagcggttg ttgctcacca gaaatttatt
     4201 aaattcaaac gtattttcgg tatcattttc ttattaccat gggcggtccc agcttttatt
     4261 actatcatga gtttttcgaa tatattcaac gatagcgtag gtgccatcaa tactcaagtg
     4321 attcctttca taaaccagct tccttttgta gatatcagtg caatctcttg gaaaacggac
     4381 ccgaactgga cgaaagtagc aattatctta gtgcagggct ggttaggatt tccctatatc
     4441 tatgtgatgg ttacaggaat cttacaatcg atttcagaag acttatatga agcagcaaaa
     4501 atcgacggag caaatgcatg gcagcgcttt tcaaacatca cgcttcctgc tgtcttccta
     4561 gtggcagcgc caacatttat tacacagtat acggggaatt tcaataattt ctccatgatc
     4621 tatctgttta acaatggcgg tcccggaagc gtaggcggag gagctggttc tactgatatt
     4681 cttatttcct ggatctataa acttacgact ggaacttctc cgcagtattc tatggcttca
     4741 gcaatcacgt tgattatctc cgtcatcgtt atctgtgtgt cattgctggt atttaaaaag
     4801 acaaatgcgt tcaatatggg ggattaacaa tgaaaaaatt tcattcgagt gctcgattcc
     4861 aaaaacgact ttctcacttt tttacttact tttttctttt aatactgtca gtcattatca
     4921 tctatccgct tttgatcaca gcaagtactg ccttcaagtc aggaaatatt gcagctttta
     4981 gtttagatct ttcaagtgac tggactttag gaaactttac tcggctattc agtgaaacac
     5041 tttacgggac ttggtataaa aatacattga tcatttctgt cctaacaatg atcgtacaag
     5101 tgactgtagt aactttggcg ggctatactt atagtcgcta tcgttttatc ggccgcaaat
     5161 caagtttgat gttcttttta gtgattcaga tggtaccgac tatggcagca ttgactgcct
     5221 tttatgtcat ggctttctta ttagaagcgc tcgataagta ctggttcttg acaatgatct
     5281 atataggcgg tggaattcca atgaatactt ggctgatgaa aggatacttt gacactgtac
     5341 caatagattt agatgaatct gctaaacttg atggggcagg gcatttcagg atttttttcc
     5401 agattgtatt accgcttgtc aaaccgatgc tcgccgttca agcattatgg gcttttatgg
     5461 gaccttttgg ggatttcatg ttagctaagt tcctgcttcg aacacctgaa aatcagactg
     5521 ttgctgtcgg cttacaaaca tttatttctg atgtgacaaa ccaaagagtc tctctttttg
     5581 ctgctggggc aattttgatt gcactaccga tttgtctatt attcttcttt ttacaaaaga
     5641 actttgttac cggcctgaca tctggcggaa caaaaggata ggagcagagt atcatgcaaa
     5701 catttccgtt aaactatttt tccgcattac gtacgccaac tcaagtgttt gcgggacgca
     5761 aactattatc atggcctaaa tttttcttga tatttgtatt tttagtttcc ttgatggtca
     5821 tgcctgtgac tcttttttat gccaatcaga tccaagcgat tccattagaa caatttctat
     5881 ccgttcattc gctgatcgat gaacaaggaa cccaaaaatt ttctgaactt gaattatctg
     5941 aaactgggtt acaagcatcg caacaaacga tagctgtgac ccctgaaatt ttggtaggtg
     6001 tgtctttatc tgagaaacaa caatccgatc atggaacttt tattgatttt gagaaggaac
     6061 aatgggtgat ccaacaaaag gataaatctg gtatacgccg ctatacgatg aattatagcc
     6121 catctttcca accagattct gtacgtacgc ccgaagattt ccaacgattt ttagaaagag
     6181 aattttatgc cagtaatcgt ccaacaatca ttttatctta tagtttaagt cttggtcttg
     6241 tcctatttgt catgacgagt ctcattttat tcggggcttc ctttttcttg tggatgacaa
     6301 gaaaaagcca actttcatcc attcatacat tcaaagagag tgcaaatttg atgctgaatg
     6361 ttatgggaat aggttccatg gcagcagcag ttgtagggtt tatccacttt gactttatct
     6421 tgatgctaag tgtccaaaca acgataactg tcttgctgct tttatgggtt tttgtcaaaa
     6481 caagattcaa agataagcgt gtcgaatgac aagtttaagc gatttatgcg acaatg
//
LOCUS       DAGCYL010000147         6481 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.149, whole genome shotgun sequence.
ACCESSION   DAGCYL010000147 DAGCYL010000000
VERSION     DAGCYL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.149"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(50..277)
                     /locus_tag="MB216_002244"
     CDS             complement(50..277)
                     /locus_tag="MB216_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527036.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            complement(444..2414)
                     /locus_tag="MB216_002245"
     CDS             complement(444..2414)
                     /locus_tag="MB216_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527037.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2429..3295)
                     /locus_tag="MB216_002246"
     CDS             complement(2429..3295)
                     /locus_tag="MB216_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527038.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3285..3518)
                     /locus_tag="MB216_002247"
     CDS             complement(3285..3518)
                     /locus_tag="MB216_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBT4527039.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(3530..5686)
                     /locus_tag="MB216_002248"
     CDS             complement(3530..5686)
                     /locus_tag="MB216_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527040.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(5706..5927)
                     /locus_tag="MB216_002249"
     CDS             complement(5706..5927)
                     /locus_tag="MB216_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527041.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(6037..6375)
                     /locus_tag="MB216_002250"
     CDS             complement(6037..6375)
                     /locus_tag="MB216_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527042.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
BASE COUNT         1832 a         1274 c          884 g         2491 t
ORIGIN      
        1 ctgagatcac ctttcaatct aaatacacgg ttcttttctg gtaacttatt catgcttcaa
       61 ccacctcata ctgttctctc atacctttga cttcattaaa aaaaactctc gctttttctt
      121 catcagtaaa gtttttatac aactgattac aataatgagg tttttgaagt ttctgaatac
      181 ctatactaac ttcaaatcct tcttcatgtt gaattaggat tacttctgtg tctctaccta
      241 gaaatattcg atctaaaatt tttatgtttg gtttcattat ttaacctcca ctataattat
      301 ttcactcaat aaggtttcac tttatgaaat ttctatagtt ctcttttcac aaaacgtacc
      361 atgttctcta agactgtttt tagtctcaaa tatacatgtt ttttcttatc ctttccttat
      421 acactcaaaa tcaaagataa aacttaaaga ataaactctc caataacaaa attattagtc
      481 agatccactg gtctcctgat ctgaaattta ttacttcttc ctgttgtcaa atactgtctc
      541 aaatcaacag aaagtttttg atcatcttct ccttttctag caggagcaac ttttttttca
      601 tacttcatta actctccact ggtcatcttt ccaatgatag tgtaattaaa atcactttca
      661 aaactttggt aaatttttcc atctgtattg aatatatcag ctgtttcaat atcttctttg
      721 tattcattgt cttctcctga accgattgta tcaaatagaa aggcaagacg aattccatct
      781 tcttcaagca ttgttaatct ttttttaatt agcccaacgg tttcaataga taacttatcg
      841 aagccgtgaa tcataacaat atcatctttt tttgcttgtg atgtgatgaa atcaaaggta
      901 tttaaaaatt gagcttccat aacattggtt tctgctctta gatcacctaa gttatagtaa
      961 aactgtaatt tgtcactttt accgatttct ttcagtgtcg ttctttgatt aaacatccta
     1021 gagaagctgt ctaatgctgt tctcaatgtg tcttttaata caataatacg atcgacttct
     1081 ttttcagtac cagatttttt ggcttttgtt tcagcattag tcaaatagct aataaaatcc
     1141 ccaagaactg gcattgtatc actactgttc accaaaagtt ttgaacgttc ttcataatta
     1201 atatcccaaa gtttctgact tatataaaag ttacctaatt cttttttcaa taaaactcta
     1261 gttgcagaat ctaatgttct gcctgctaac aaataaaaaa tctgagttag tttatcgaga
     1321 ttccgtctat ataaatcacc aatgtccttt tgattaccaa aaagctccaa agagttaaga
     1381 ccttcttcag ccaaattaac cttttctatt acactgttta acgaaggttt ggcaacaaat
     1441 gttctaccag ttggatcacc aaagtaatca aatttattta agacaatatg aaaagttcgt
     1501 ttatgatttg ccattgcatt attagcaatc aattgccccc aaagtgatga tgctgagatt
     1561 ttttcatcat agcctttaat tgtagcgttt ttgtgtgatg ccactaaaac tttacttgta
     1621 aaagaatcat ccagtgccat aaaagctaca ccacttgcgt agctagtaga catagtgccg
     1681 atagcccatc cattcgcatc atctaaacct ttgcgaattg catgatcatt tcctgcaaaa
     1741 tccgaactca tagacgtata ctcatagaca ttcccgattg gttcacttaa aagtctatca
     1801 aacagatcac gttgatctcc tccatatgac ataagcttta atccatacat tttttcatca
     1861 tacaaccgtt ggagtttaat tagctgttcg ctgattttat taggagaatc ggatactaat
     1921 aggatttcta gcttccaatc aataatttta tttgccatgc tgtctagtaa agatgctgta
     1981 ttcaaatcca taatccgcat aacagttttg ctaatttcag catctgaatt ttttccttct
     2041 aaattatttt cataactatt cgttgatccc ccaacattct cgtgcattat ttcttgttga
     2101 tctttttgtg acactggctt atctgcttca attagatagg cttttacccc ttctactctc
     2161 acttcaggaa tagctttaac catccaacca aatccataat ttctattcat tccataagta
     2221 ttcactaatt tcacaattgt tccccaccgc ttacctgtcc taatgtaaga tggtgtaaat
     2281 tgataaccaa ctaaagatgt taaatcacta acaaaggtat tattcactac ctttgaattt
     2341 ctagtttttt gttctttttt agtttttctc ttttttttga aatttaatag ttgtgatcta
     2401 cccataattt ccatttaatc tctcacctct atttcagtgc ataaatatca gataatattt
     2461 cttttgtttg tttagctgac aactgagtga cactataata aagtcctcta ctcgtatagt
     2521 tttctaaacg ttccacacct tcatccaata ccatcttaga ttgagatttc aagataagat
     2581 attggattac tgttgttttc acaccatcaa tatattctga tacaaatcgt tcttgctgat
     2641 ctattatgtc aaagactgct ttgttactcc ttgcatttcg tttttgtatt ttcatatttc
     2701 gagtttgtct ttccgtattc tgaggttgag atgaagtgat tttgatttca gctaaggaac
     2761 gatctctggt attttgatat tgtattgatt tcgattcctg agccataact tcttctggaa
     2821 aagcggttag tgatgtagcc ccatctagat tgaacattaa tgctacactc ccatctcgat
     2881 atacgattgt tccatcttgt ttcactttta aaatctctgt gcctcgaaat ggtttctttt
     2941 tccccataga tactcttgct gaattaacta ctgatttgaa cagtctaata gaaccagaaa
     3001 tcaatgaacc actagatgga tcaataaaca tatactggct aattagaata agaaatccaa
     3061 ttatcattaa taacgttgga acaaaaataa atttcagttt aatcgtatca gaaactaagt
     3121 aaacaattga tccaagaaca acgactgcaa caactatagc tgatacaatt aatctttttg
     3181 ctgtaacaga ataggtgcta ttgacatgct tttcaagcca ttcaactctg acaattgttt
     3241 cagaaggttt ttgatgtaag ttatgtttaa atccatagtt tgagttatct gccatcagaa
     3301 atacttcctt tcacgttcaa cttacggtaa acacctacag ttatcactat tgcaacaata
     3361 agcaagccaa ttaaagatat tgacttgctc attgtctcgt tcgtttgtgg tagttcattt
     3421 acctgataca cgagctgtgc ttcctgtaag tgctgagaag aggtcatgtt ttcttgattg
     3481 ttttgaatta ctggataaac tattttttct tctcgcataa tagccctcct tagttattat
     3541 cagataagtg agcttgaatt ttgtactgat ttccgtctgg agttttcaca actgcttttt
     3601 ctccatcaat agaaatcact tcagccttag ttgttttccc atcaatactg atgacaacat
     3661 ctttcccaac tttgaactct gtagttggtg ttgcttcata gctactattt gttgaagcag
     3721 agttcgtatt tgtcccaatc gaattttcca ctctcgaaat agatgtttga gtttgagcct
     3781 gtgcttgttg attattatga ctaataaagt aggacgatcc caagatcgtt ccgcaagcca
     3841 ttgcagcgac agaaatgata gtagccatct ttttacttgt aaaatcacgt tttgaacttg
     3901 attgattttc tgtagcaact gctaatgatt tcgttttttc tgccatttga gctagatcac
     3961 ttcgggaacg agataagtct tctcttaaag cttcactttg ttctgccaac ttttcgctat
     4021 ttaatttata accctctgct aaatctttga cctctttcat tcgatcttta tattcttcga
     4081 tagttttttg ttgctctaca agttttgttt ctaattcttt cgtgttgtta gctacctgct
     4141 tttcaacttc ttctttgttc gcaaaatatt tgacaaccac atctgttgtt ttctcttttg
     4201 tatcatggaa ctcagcttca ttttctgact ttgttttttc gacaatctct aaagtttttt
     4261 cttgcacgtc tgttaatgat ttttcaattt ctggtttcaa tgaattgata attcgttcca
     4321 ccacaacttc ttttgcttct tggctttgtt tcaatgcatc gtttttaata gattcaattg
     4381 cttcttggta ctgttgatca agactttctt cagttgcttt tctatattca tcttcttgtt
     4441 gtttaacttc tggtaaatgc tcagcatcat accgttgctc tagtttcttt agttcagcat
     4501 ccaaataagc ctgtttcgcc atatcataat ctttacgaat gatattggat ttttccatca
     4561 ctaaatcatc aattttagat gcttctgctt cgtagctctc tttagcaacc gatagtgtat
     4621 ttgtaaactg attttgacca tttaatgagg ttgcttcctc tatccgcaaa agctcatcag
     4681 ctaacatttg ctttcctttg aggatcaatt gctccttcgt cttatttaat tcactgttga
     4741 ttttattatt agcaatagag atgcgagcgt tcatactctc tttagctaaa aacagcaatg
     4801 gtttcaactc aggtatggat agcatctcct cgtttaaatc gacagggtca ttaatctcat
     4861 ctaaaactac aggttgaaat ttgtcagcaa tgatctgttc aaaatttttt tctaaaaaat
     4921 cagcatctaa gtttttcggt acatcacttt cagatgtgat tttttcagct tcataaacat
     4981 aattttcttt gtcactttgg gattcgggtt tttcatcaat ttttggttgt tggtaggttt
     5041 ctgtttgtat ttgtgtttct tctatgctta cttcttggtt ttctgcttcg gttggtttgt
     5101 tatcatccac aaaaagagct tctttttcat ctggtacatt ttctgctacg gcttcaaatt
     5161 caacttcttc agaagagttg aaggtctcag caattcgttc taatccatta gtagctactt
     5221 ctaattgctc tactgcgcta tagaagtctg tagcttcttc aacttctgat tcaaacacat
     5281 taggatcatc cggaataact aataattgac cactactatc cataaaacgg acacctgcta
     5341 atagcttctc tttaaatttt tctggtagat cagctacata attcaaactt gacttcaata
     5401 gaacgacata cttctcagaa tcgagaatag catcatcctc atctataact ccctcactgt
     5461 tgagcttttg tacatcttca attaccatat aatttttctt tggtaaatct ttggtcagca
     5521 acgcatcgaa ttctttccat acctttgctt tagctttttt tgccttcgct tctttttctt
     5581 cttttttgta cacttcgagt aattgtggct tcaaagagag tgcaactaca ggaactgttt
     5641 ctgccacata attaatagat tcggtaatat ctttgaattt tgccattatt gtaaaactcc
     5701 ttttttcagt caatagttct tttctgttta tctagcatat atacatatgc tttttcgaat
     5761 ttaataagcg tttcaagtct ggctttttca atctttttac tcccatttcg ataatcatag
     5821 aactggttaa cattcatatt cattatcttt gccagttcgg tacctttcag actgctcatt
     5881 aatacttcgt tggcgtgagc taacattttt tctctatcaa aaaccatctt acatttactt
     5941 tcttccctca cttctaaact acgcttggag cgtagttttt tggtaaaaag taaattgaac
     6001 aaatcggcat attatccaaa tttgttcaat tcacttttaa cctaacagtt cattttttgg
     6061 aaattttgaa actagataga taccaacaac tggaataagt gctgatatga tattagcttt
     6121 ttggttcaaa tcattacctg ttttagtacc cattgtgtaa agcataacgg ttgtaattac
     6181 aggaataata aagattgcaa taatcgcccc aacatatttt aatccatctc cccattttgc
     6241 atcaccaaaa aatttcattg cagtgaatcc tggtttcaac gaaaaaacga atgccaagaa
     6301 atacaaaaca acttgtacta aagttttcat atcaggcgtc attccgctaa ccaaattttt
     6361 aatagaatcg aacatataaa aacctccttt ttaatatttt taataagtta attatatcat
     6421 cgacaaatct tatttgttca cttgtcaatg atctaactaa gaaaaagacg tctcgatatt
     6481 t
//
LOCUS       DAGCYL010000148         6479 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.150, whole genome shotgun sequence.
ACCESSION   DAGCYL010000148 DAGCYL010000000
VERSION     DAGCYL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.150"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(341..3490)
                     /locus_tag="MB216_002251"
     CDS             complement(341..3490)
                     /locus_tag="MB216_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBT4527043.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3503..4702)
                     /locus_tag="MB216_002252"
     CDS             complement(3503..4702)
                     /locus_tag="MB216_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBT4527044.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(4692..6287)
                     /locus_tag="MB216_002253"
     CDS             complement(4692..6287)
                     /locus_tag="MB216_002253"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBT4527045.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
BASE COUNT         1798 a         1343 c         1061 g         2277 t
ORIGIN      
        1 ggttacatgg tatctgaaac agctaaaacc ttgtcattac agcaaaaaat cactttatct
       61 ccgtttcatt tcagatacat ttcacttaag cggattccaa aaaaacaaaa gaaaaaggaa
      121 cccctagtta gcctatgctt tttattggtt cctctcaaaa cacggtcata taacaacggg
      181 gtggggtaaa tgaatgaaaa aggtggagga aacattattc aatagctgtc ttaaatactt
      241 cgttataaaa tgtgaacaaa taaaagacat ctatccctat tttctacact tccactttac
      301 taccatcttt gaatcgtaca attttcttaa ctcttctcgt ttacctcaat tcattttcaa
      361 gtggaatcag ctcatcatct aacattttac gccatgctcg cttcatttct tgtgggtatt
      421 taaatggctt cgcttctgga tggttggctt tatatccttc gtaatcctta ctgttgataa
      481 ttgctttcat attaggaatt ggatcttcac ctggcatata ttgcagatta gaagcatgta
      541 attctttctc agaaacaaac cactcattgg aaaagacatg aatcaaatca tcactcgctt
      601 gtgtaaaata gcgacgttta atgacaaaga tatcttcatt actatctaaa gtccccacat
      661 caaacgcatt taaaatttgt cggtaaactt ctttgactcc ttctgtttta ttcaatttag
      721 ccaaagcttt ttcaatttct tctcgtgcat ctgaactatt cgccgcataa gttcctagta
      781 attgatcaat ataagtagaa tcaatatcat acaacttcgt ggtattttca ctataaaatt
      841 cgatcgtgga aaaatcttct ccctcttttt ctggttcttc ctctattaat gaacccttca
      901 cggtctcgta cacgccaatt tgttcttcca aaagttgaat ttgattgttc aaggttgaag
      961 atttcaccat gtcatcattg taatcgtcat aagtcactaa cgattcataa gcacgattca
     1021 attcttggaa tgctttgacg tactcgatac ggatttcaag tggcgtatgt tcatcaatgg
     1081 attccatgga tgaaacctct tttaacttac catatgcttg cttgaatctt tttttggatt
     1141 cctgataact tggataaatc aaacccgatt cttcttttgc ttctgaatac agttttgtcg
     1201 catcctcgac attcttctcc attgtggctg gctttcggaa agtaacaatt agtccttttg
     1261 ttttttctgg atacgtacgg ttcgtacgtg aaaaagcttg gatcaatcct gcatactcta
     1321 gattacgatc cacaaatagg gtttgaatcg taggtgcgtc aaaacctgtc aatagacgat
     1381 ccactacgat cactaaatca atttgtttcc caaattgttt gaattccgct ctttttcgtg
     1441 ccaaacgatt attgatgtct ccgttataac gctcaatgtc tgctaatgac caggaagtat
     1501 cataatactg attatattcc tcaataattt tttgcatttc ttcttgttgg gcacttgaat
     1561 ctcggctatt ttcttctaat gaataggtaa tagcgattcg tggaaaatca ggatcctcca
     1621 tcgttcgtcc ttcacgaatg agctgattgg gaaactcttt tgttaaccaa tcaggctctt
     1681 tcgtcatctc tttgatggcc cgataatact ttttggccat gtcaatggaa ctcgtggtta
     1741 agattgcaga tttcgttggc ctgccatttc gaaaatcaaa ttttgtatag gcattatctg
     1801 gacggaacat tttatgaatg actttttgga tatgttcatc tgcttcaaaa acagctgggt
     1861 ccaaataact ttcttttttg acaggttcca tttgatcaat catacgattg atctgttctg
     1921 atgaatactc tgcatatgtt tctacttctt ttaatttccg atagatttta ttctctagtg
     1981 aagtcggctc cacggtgttt tcatgctcta cttgaaatcc taagacagag ccatcttcta
     2041 gcgcattctt aatggtatag gtatgcaaga cctctccata ctgatcatgg gttgtacgtg
     2101 ccaattgccc tttggcttgt ttccgattct caggaaaaat cggtgtacct gtaaagccaa
     2161 accaagtgga tttcggaaac atttttttga tttcttccat attttcagca cttaacgctc
     2221 gatgacactc atctactaca aacacgatat gttggcccat taatttttgg aagcgattgg
     2281 ttcctttttt ctcttcttgt ttctttgcat atttcaacgc cgcatctagt ttttgacggg
     2341 ttgtaatgat tacaacattt gcattggcat ctgccaacag ggtttcactt aattctttgg
     2401 cacttcccgt tccaacgatc aagctattcg ctttggcgtt cccggaagaa atcccagtat
     2461 taaattccga agcaaatttt gtaaactctg tggtggtttg attatctaaa tctttgcggt
     2521 ccactaacat gatcgttcga tcgatccccg attttcgtgc taaaagctta gtagatacaa
     2581 aactagtcaa cgtctttcct gaaccagtcg catgccacac ataccctgat tgatgtttat
     2641 ttgcggcaat aaacaaggct tgaatcgcat gtacttgata aggatgtaac accatgaggg
     2701 ttttattatc ttgatcttca ctgacgattg tataatccgc aatcaaacga tgagcatccg
     2761 ggatatttaa cacttgttta caaaactcat ataagttttc tactttttca ttgtctttcg
     2821 ttcgccaact aaataaaaac ttagggtgca agtctttcgg taaggcattt gcaaaatacc
     2881 gagtggtttg ttcattggaa accacaaaca actgtaaggt cgaaaagata ttgttccgaa
     2941 acataccttc ttctgcatat tttttgattt ggttaaatgc ctgataaaca ccatcttttg
     3001 cagttacttg tttcagctcg atttggacaa tcggtaatcc attgattaat aaggtgacgt
     3061 caaaacgtcg atcccgtgct tctatgttac ttccacgttt tgcaatttga tggaccactt
     3121 cataagtaga gattcctcca ccaatgtctt gattggaata aaggataagt gagacagaac
     3181 ccaattgcga atcttcccgt tcaatcgtga tccgagccat cccattttca ccttttaacc
     3241 atttcgcagc atcaaatggg gttttcgttc gcagcagtaa ttccgttttg atcgtttcaa
     3301 attctttatc ggtcaaagga aaatcattca gttctgcttg attatttgag ataatttttt
     3361 ggcgtaaatt gacccaaagg tcttcttctg attttagatc cggacgatac gtccactgat
     3421 tatgcccttc tccaagtaca cgaatcaact gttcttcgac ttctgcttcc tcacggtgac
     3481 taattttcat tcttcttccc tcctagacaa acatcttttg taagaagctt tttttcgttt
     3541 cttttagtaa atctaactta cgttgatgaa gagcgatcgt atcatctagc tgtttgaaga
     3601 aagtaccgat tttttgttgt tcatccttta caggaacctt aacactgata ttctcaatat
     3661 cagaactgtt ataagaaggc aatgccccta cttgggagta tcttggcaaa tttatagttt
     3721 caaacaatat tttaccaaaa tagatatccc aagtattatt aaataaataa gccatggtat
     3781 tgttatctat taagaaaggg cttgtgacca aacgttttct attaagcatt attgctgcgc
     3841 caactttagc aaatataatc gctggcacat cttttattgg cttccatttt ttacgctcaa
     3901 tttgctcatt agatacataa tgatttgcat ttctcatttc atactcatta ccaatgttat
     3961 tcatgtcgga aactttaaaa aatggagtac cttcagaacc accttgctcg gcatcaggaa
     4021 atccaattcc cgattgagtt ttacctaatt ctcccaactt acgctcttcc caatcttccg
     4081 taaaaccagg aaaccggatt tctggcactt ttgctccgtt ttcagggaac attttttgta
     4141 agaatccttt tttcgtttcc ttcagcaaat ctaacttacg ttgatgaaga gcgatagtgt
     4201 cgtcgagttt tttgaagaat atgccgattt tttgttgttc ttttaaatca ttgggaatat
     4261 agaatactgt atctgaaact tttttccagt ctgaacgagg catttttgtt ccagatgtat
     4321 catttgcagc cttttgatat ctatcagctt gaataagaga ataaataaag tccgagtcgc
     4381 tcttcttagc tctaaaaacc cagaaatccc caatcgctac gcctgtaaaa gttgccttga
     4441 gccaattttt taaatatgga cgtaactttc catatagaat atcatttggt aaaaattcaa
     4501 tcccttttct attatcaaat ttctggctca catctttatt cagtctacct tctcctgcca
     4561 cgatatcttc aaattcaact tttggaagtc tactggagtt tgtactttta ttaagtctaa
     4621 caactaaatc aattaactta cgctcttccc aatcttccgt aaatcctggg aaacggattt
     4681 ctggtcgtgt atcattgctc atgtttgaac acctccagcg caccttgtaa ccacatttcg
     4741 ttttcaggtg ttgtttgcag ggaagagata gcttctaaaa gttctttttc tagcacttgc
     4801 tgttcttgtc gtatcttctt gatgtcattt ccgacatgaa ccatatcaat tggttcttct
     4861 tcttcgaaag tatctacata gcgtgggata tttaagttat agtcattttc tttgatttca
     4921 tcaaaggttg ccaaatgagc gtatttctcg acatcttttc tttcagcgta ggtttcgagg
     4981 attttttgaa tgttttcttc cgatagttta ttttgatttt tccctttaac aaattcacga
     5041 ctagcatcaa tgaataatac atctcgagtt tgacgattct tcttcaacac aatcaccgtc
     5101 gttggaatcg atgtcccaaa gaacaagttc gcaggcatac cgatcacagc ataaatactg
     5161 ccatcttcta gtaatttttg acggatgacc ccttctgcag cgccacggaa taaaactccg
     5221 tgtggtaaga cgatggccat ggtacctgtt tccttcaaat gatagaaccc atgaagaaga
     5281 aacgcaaaat ctgctttgga ttttggcgct aatttcccat aacgattaaa ccgagaatca
     5341 tctaagaaag tcgtatctgc tgaccaatta gccgaataag gtgggttcat tactactgca
     5401 tcaaatgtgt aaggttcatc tgttggccag tccttattca acgtatcccc attgcggaga
     5461 ttcatttctt cggcgtcgac accatgtaaa atcaagttca tttttgctag attgtatgtc
     5521 gttgtattta gttcttgccc atgatattta acgttatctg ggtgtgttaa gtagttacgt
     5581 acgtttaaca tcaatgaacc tgaacccatc gttggatcaa aaacactaaa gaatcgtcgt
     5641 tccttttgat cgagcgttac gatttgcgcc atcatatcag aaaccatatg aggtgtgtaa
     5701 aattctccgg ctttcttccc tgcttctgaa gcaaattgac tgatcagaaa ctcatacgca
     5761 tccccgatca cgtccccatc gtgttctaaa acatctacat cattcaattt cttgatgact
     5821 tcggtaatcg tcacatttcg ttgttgctca tctgttccta gtttttttga ttggagatcc
     5881 acatcatcaa ataacccatt aaattgattg taggtagatg ccaattgcac aaaggctttg
     5941 tttagatcgt tcaattggaa cgttgcttgt tttgcttgat ccgctaagac gttgaaaaga
     6001 tactctggtg caattgcata tcccaaaata tcaacgattg tggcaatcag atcttctttt
     6061 gattcttcat ctgaaagtaa ttcacgatac aacattgttt gtttgcttac tgtgtcatat
     6121 tcctctaagg attcatctgc taaaagaacc acttgttcca acaatcgatc ggataaatat
     6181 ttatagaaaa tcagtcccaa taaataattt ttatattctg acgcatccat tttactacgg
     6241 agattatctg ccgcactaaa taatttttga tttaattctg cacccaaaaa gatacactcc
     6301 tatctcttat ttaaagttgc tatataccct cttattttag cataccccct tttataaaaa
     6361 gcaaaaaaaa aagaaagaaa cttattcttg ctaataaact acgcgaaagg agtctaacgt
     6421 tgtttgatat aacttagact gatcgttaac ttatttttta tatttctaca tagttatct
//
LOCUS       DAGCYL010000149         6432 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.151, whole genome shotgun sequence.
ACCESSION   DAGCYL010000149 DAGCYL010000000
VERSION     DAGCYL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.151"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(99..1229)
                     /locus_tag="MB216_002254"
     CDS             complement(99..1229)
                     /locus_tag="MB216_002254"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBT4527046.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="MB216_002255"
     CDS             complement(1878..3080)
                     /locus_tag="MB216_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4527047.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="MB216_002256"
     CDS             complement(3140..3343)
                     /locus_tag="MB216_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBT4527048.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="MB216_002257"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="MB216_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="MB216_002258"
     CDS             4880..5692
                     /locus_tag="MB216_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4527049.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1930 a         1313 c         1008 g         2181 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcataga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatattttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt ca
//
LOCUS       DAGCYL010000150         6430 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.152, whole genome shotgun sequence.
ACCESSION   DAGCYL010000150 DAGCYL010000000
VERSION     DAGCYL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.152"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(1062..1727)
                     /locus_tag="MB216_002259"
     CDS             complement(1062..1727)
                     /locus_tag="MB216_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4527050.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1907..3889
                     /locus_tag="MB216_002260"
     CDS             1907..3889
                     /locus_tag="MB216_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBT4527051.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3893..4531
                     /locus_tag="MB216_002261"
     CDS             3893..4531
                     /locus_tag="MB216_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4527052.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4559..4987
                     /locus_tag="MB216_002262"
     CDS             4559..4987
                     /locus_tag="MB216_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527053.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5444..6355
                     /locus_tag="MB216_002263"
     CDS             5444..6355
                     /locus_tag="MB216_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBT4527054.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2226 a          978 c         1185 g         2041 t
ORIGIN      
        1 ataaaggcta ggaacctgat aacatcaagg ttcctagcct ttttataata aaatgaagcg
       61 aaaataattt cttttaaaga gccgagatta tcagctatat aagagtgaat ttgatgcaaa
      121 aaacagagcc gaaacagtct tggtgttcgt agctctgctt atatgtttcg aatttaagac
      181 aaagagttgg caaaagatgt tgataaggaa gataagataa ctgtgttata taaactaggt
      241 aactaactgt aacaagctaa ttttaagaga ttcttattag aagacgttta ggaatataac
      301 taagaaaatg attatacagg taattgtcat ttcaataatg ttttctaatt ttcgcgtctt
      361 tgttttaaga aaaaggccag aatcagttgg aaaaaagttc gtacgatagt gaagaggggc
      421 aaaatttcct actaatattc ccactaacaa gagataaaac ggccaggttg gtgtgattcc
      481 tctataaaaa aatgcccatg ctaccgaaat cccatagatt aacaactgaa caatttttac
      541 aatgaaaaaa ttgttctgcc attactcaaa gtctcttcct ccttgtatcg tttatttgtt
      601 tcattttatc attatgatag gcgcttgaac aatccatttt gtattttatt gagtactctt
      661 ggataaacta ccagcgatta tcatatttat ttatgttact gtaagccatc atataaaagt
      721 aaatgagcag atcatagaaa tataaaaaag aactaaaatt gatctaaatt atgtgcagct
      781 tgactacgta ccaatctata ttacttacta gattggtcgt taatctattc ggatcgaatg
      841 agcaaaatat ttttgtgaat gtccatcctt gattatgatt ccaaaaagaa tcattgggag
      901 gctgtggcgt aaatattata ctataagcca gaaataccta tcattctgaa cttttttatt
      961 taggaacttt catctcaagg ctttttcaat taatttgtac ccctgttttt tggataagat
     1021 catgtgcatt gattttttct atctctacgt tcatctccta cttaatgcct atctggctcc
     1081 tgagggcttc tttttaaggc gtcgtcatag tcttggctga ggatttcttg gtaatctgag
     1141 ggaatggctt cccaagtgag caggcgaacg ttcatgacgt gttttccttt gtgtttgatt
     1201 acagcatggt ttttctcttt ttctacctct ccagcgtgaa aagacagcca tgtaccatgg
     1261 ccaaagatat cattacatac ccaacgttcg atggaattgt ccacgtatcc tacagggagt
     1321 gtggtatagc cgatttgtgg tttcaaaaaa ggattgaatc gatcgatcgt ttcatttttg
     1381 tattgataat tgataagaaa aagatagacc aaacaaaaga cgagcagaaa gaaagatagt
     1441 gtctttttga gtgagaaggg ttgttttgat gaataagatt tcttgttaac aagaggttca
     1501 tctggcaatg ttggttctat tgattcggta gctgattcaa tagaaggaaa atcaatcggc
     1561 agacctaaag tatgatgtaa aagtgacagc atttcaaggt ctggcattgt acgagaattt
     1621 tcccaactag aaatcgtttt atcggaaaca ccaatttttt cgcctaattc tttttgcgta
     1681 agcttacaat ttctgcggta agttcgtatt ttttctccgg tgttcatctt cttctccttt
     1741 cgattctaca gtatatagat ttattctaca tgacctatac acttgataga tacgtttttt
     1801 tgatgaaaaa cggtatgatt ttatcaaaga atcatttaag taagggagag tggtgataat
     1861 caaaaaaaag ttcacagttt tactcacaat catagtgacc ggtatcatga gtggttttgc
     1921 cttggttagt ataaataaac aggtactcta tcggacaaat gaatttttcc atcaaacaga
     1981 tacttatact ccgattcgat tgccacaaca ttttattggg caagaagaat atgagcagct
     2041 aatcatggta tttgaaaatg tctcatcaga aactgagctt tcttatatga aaaaaacagt
     2101 aaatgaatcc aatgaagtag atacatcttg gaacttcaag atacagccaa agcttgaaat
     2161 gacattgtat tatcaagggg atgtcaaatc ttctcacgag aaggtaccct attctaatac
     2221 ctcctttcgc gtaacgtcca ttgaagaatc gatcaaaata aaagattttg aaggtgaatt
     2281 tttcttgatg acgagagaaa aagaagccta tgaacggttc atttcactat tcactgatcg
     2341 attcaatcaa gcatttcaca ctgattatgc cactaatatt tttgaagact ttgaaaatag
     2401 ctattcgtat tcggttattt caaatacaga cgtatataat tatcgagtgt atttaactat
     2461 tggattgatt cttttattcc ttttgttatc atcctttttc ttattgatga ataaagaaat
     2521 cagtttattg ttgaataatg acttttcatt acatcaagta ttgtggtgtt tattggggaa
     2581 aaagaaaatc attattacat tcataattgc aggaatcatt ggtggtttta tttttatgta
     2641 ttataaggag caatttcttt atttgttcca gatgtacgtt ttcttgttca gcggaatata
     2701 tggtatctct ttgatttctc ttttattagt tgaaaaaatc gcttcgttaa gagaagcgtt
     2761 caactatatc caatacattc tttttacatt tggattgttg ttttctcttc ttttgaccgt
     2821 tgccaatgtc gaattagcaa ctatcgtttt ttcttcttca cagttagctg tttcccatga
     2881 accaccaaaa gaaatcgctg gtgaggacta tcatgtattt tatccagtaa ccatcgggaa
     2941 aaaccacgtg gattttattt attccaatcg ttttgcatca gcagcagatc aggaattata
     3001 tgaaactttg aataaaaacg gaagtatatt agtcaatgta tcagattact tgaatgaaga
     3061 aaatgaacag tttggcagag ggataaaaat aaatctgaat tatcttaaga aatttccttt
     3121 gattgatgtc tctggaaggt tgatccaaat aaccgaaaaa gaaacgcacc ctgttatatt
     3181 gatccctgaa cgatatagac atacagattt aaaaaatgac ctggcgcaaa taacagaata
     3241 ttatcaagaa atcagtgaaa aagaaccgaa atttctattt ataaaaaaca atcaacccat
     3301 ttatacattt attccttcta tcccctggat cgaacattat ccggttgttg aaatactgac
     3361 attgaaaaac agtatgtatt tggaaagaaa tattctcagc ggagaaatct atccaccgtt
     3421 aaagatcaag ataggctctt tgtcgaaaga aaggttattt gagctgattg aagaaagtca
     3481 gttacgtgat aacttgcagt tatcatttcc gtatactcag actgaagaga tagctgtcaa
     3541 agtgttaagc agatcatttc attacttgat acagatattt ttactcactt tcttttcttt
     3601 cttcttgttg agctatgtca tgttatgcat atattttgtc tacaattgtc ggaaaatcgc
     3661 tatctttcgt tcaagtggtt attcgctctt tgaaacgtat aaagatttct tcatgatgaa
     3721 cctcatcaaa tggggaacga caagtgtaat cttccttttt cttatagaaa gagagcctaa
     3781 atatcttttt aatatttttt tctttagctt cattgatttt attctttcag ttgtttttat
     3841 tttgtctaca gaaaagaaaa gtcagttatt gctgatgaat ggaggttaac aattggagcc
     3901 gttgatagtg ataaaaggtg cgaaaaaaat cattcgaaac aaaacgattc ttcatttgga
     3961 tcgtttcact gcggatgctg gggatcttgt gactgttgtt ggagaaagtg gagcaggtaa
     4021 aagtacgtta ttgaatatat taggattgaa tgatcagttc agttcaggac aattctattt
     4081 gttcgatcaa gagacgaaac aattaactgg gagagagaag cgcttgataa aaagaaatac
     4141 catatctttt ctttttcaag attttgggtt gattgaatat gaaacaatcg attttaattt
     4201 agatattgga ttgaagtttt cgaatttaaa tgtttcagat aagcgaacag caaagtatga
     4261 ggcattgaaa aaagtaaata taaacaagaa gctaagtacg cctatctaca cattatctgg
     4321 tggtgagaag caaagaatcg cctgtgcgag agtcttattg aagaatagca aaatcatttt
     4381 agccgatgaa ccgactgggt cattggataa aagaaataag gataaagtag ttgaaatctt
     4441 atcgcaacaa gcttcccaag gtacgttagt tatcgtagtc acgcatgatc catatctgac
     4501 caaaatagga aacaagaaga tcatgcttta aaaatcaaaa aggaaaggat gtcgttttat
     4561 gaaaaaagca gttattattt gtgcactagg gataggtttt gttggaggaa acgctatgtc
     4621 atctgaagtg gaagcaaatt cagttcacgg tgaaatctat tacaaatctt taaagccagg
     4681 agagattcat tttgatgcta aaggtaatat tattaagtca aatttaccag tagaccggga
     4741 aatttctaga acagcaatta atcatagctc tggaagatgg gtttattata gtgatacaac
     4801 ttgtacgaga aaaacaggtc attctaacca ctatagtcgt gtctataatc gtcacggtgc
     4861 aaaagcgaaa gttggaaact ctattcgatc agcaaatgca acaaaaggag tatgggccta
     4921 tgcaagtgcg acaggaaata gaactgacac attctcatgt tggtacaatc caaccggttg
     4981 gtattaagta tccaaaagtt tatataaaat agaaaaaaca tataatgaaa cagcgttaaa
     5041 agttattttt ggtagtttta atatattcat ttttagttgt ataaaaatat atcataataa
     5101 aaacaacaaa aaaatgtctg gaaaatcgaa cataaaagtg tctgatttga atatcccagc
     5161 agcggaagaa gaaaaacaaa atcgataaca tttgttctaa gaattagtta cgttaaatac
     5221 ggaacgcaag atagacttga aaaacggtaa caaaagaagt agtatattaa ggtgtgatgt
     5281 gctacttgac atttattctg ctgagcgaac tttctggatt gtgtagcctt atatctattc
     5341 aggacgtatc gagcagatta gtaaaggtgt tcttgatgat tttcatcttg aacacctttg
     5401 tttttagcaa ttcgcaaaaa acgaaaagta ggagaattga taaatgaaaa aaagattttt
     5461 agcacttgcc atcgtactag gcactggctt attgtcaggc tgtacgaatg ccggtgaaaa
     5521 aacagccgtt agttataaag gcggtacaat cagcgaacaa gaagtaatgg atagcttgaa
     5581 aaaaatgcaa ggagcagatt ccgctgtgca acagttgatc gtttatcaag tatttgaaga
     5641 taaatatgga gatgacgtat caaccaaaga aatcgattct caatatgatc aaacaaagaa
     5701 acaattgggt gattcatttg attcacaatt gaaatctgca ggttacacag aacaaacatt
     5761 caaagacagt atcaaacaaa gtctagcctt tcaagaaggt ctaaagaaac atatcaaatt
     5821 gaccgatgaa gatttaaaaa cggcttggga atctttccat ccagaagtgg aagctcaaat
     5881 tatccaagta gcaagtgaag atgatgcaaa agatgtgaag aaagctgctg ataagggaga
     5941 cgatttctct aaattagcca aagacaagtc aactgatact acgacaaaag aagatggcgg
     6001 aaaagtgaaa tttgattcca ctactacaac ggttccggca gaagtcaaag aagcagcctt
     6061 taagttgaaa gatggtcaag tttctgatgt gatcacttct accaatgctt caacttatac
     6121 tacggaatat tatgtggtta aaatggtgaa aaaccaaaac aaaggcaacg acatggataa
     6181 atacaaaaaa gaattgaaag aaattgcaac agacacaaaa ttaagtgaca gtaccttcca
     6241 gaataaagta attggcgaag tattgaaaga cgctaatgta aaaatcaaag acaaagactt
     6301 tgaaaatgtt ttgagtacat tcacttcaga ttctagtaca gcaagtagta aataagcggt
     6361 ataaataaaa aatatgaact atttaaatca ataaaactgt tcatctaaat ctttttatta
     6421 gaactaaatc
//
LOCUS       DAGCYL010000151         6408 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.153, whole genome shotgun sequence.
ACCESSION   DAGCYL010000151 DAGCYL010000000
VERSION     DAGCYL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.153"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            361..642
                     /locus_tag="MB216_002264"
     CDS             361..642
                     /locus_tag="MB216_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527055.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            644..1060
                     /locus_tag="MB216_002265"
     CDS             644..1060
                     /locus_tag="MB216_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527056.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            1682..2836
                     /locus_tag="MB216_002266"
     CDS             1682..2836
                     /locus_tag="MB216_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HBT4527057.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2830..3252
                     /locus_tag="MB216_002267"
     CDS             2830..3252
                     /locus_tag="MB216_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527058.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            4422..5390
                     /locus_tag="MB216_002268"
     CDS             4422..5390
                     /locus_tag="MB216_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4527059.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            5407..5931
                     /locus_tag="MB216_002269"
     CDS             5407..5931
                     /locus_tag="MB216_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBT4527060.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            6107..>6408
                     /gene="tnpA"
                     /locus_tag="MB216_002270"
     CDS             6107..>6408
                     /gene="tnpA"
                     /locus_tag="MB216_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBT4527061.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT         2414 a         1095 c         1046 g         1853 t
ORIGIN      
        1 ttaataaaaa ttgctggttt tattcttctt tttgcttttg cgagcagagc aacatggacc
       61 catgttgcga tattttggtg gcgccccaat ccccgttttt tcgttgaatc ttactcggac
      121 tgcactcgtt ttacagctga tttttctgaa ttttgaaaga gcataaggaa acgtggtatc
      181 actttcttac gaaaggtgct agataggctg tgcctatcac gtttctcaga aaaaaatttg
      241 ctatcacttt tttgccattg tggctaggct atgtatagtg ttttggcgaa aaatagctat
      301 acaaatacta tatttgtatg gtatacataa attatacgct gatacgaaag gaaaatttcc
      361 atggacattt tagttcgaaa tatcaatccc aaaattattg caagaataga taaacgttgt
      421 caggaactca caaccaaaac gggcaaaaaa tggagtcgaa ataaatatct aaaagttcta
      481 atcgaaagtg attttgaccg tcaacttatg aattacaaaa aagatcaatt cgatttatta
      541 ctcgaaaaat ttattgccat tcaaacagaa aatacgaaca tactgaatga ctatgttcac
      601 acaaacaacc aactcattga aacacttatc ggaggaatat aacatgagaa aaacttctgt
      661 tcaaaaacac gtaacccttt atcccagtca cgttgacgtt ttagattcca tcatcgaaaa
      721 aaatccagac gtcaacaatt attcagaagc cattcgtttt ttatgtatga gttatgaacc
      781 tgataccaat acaaccacaa ccagtaacca agcaaaactc aatgccatgg gaaaagaaat
      841 ttctatcatg acagaaatca tggcagaatt tgctgattta tatctaaaag atactggtgt
      901 tcttatcggt acaaattcta aagtgtatca agaagtgaaa gaaaaaattg aaaatcaact
      961 caatccttcc cgacgaaatc aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac
     1021 ttctggaaat tttgacccaa caaactttct aaaaagataa aaaaagaaac cgatccctaa
     1081 aaaaattcct cgatttcttt ttagggatca gtcttccagc aagatttgca agttggtacc
     1141 aacttgcgaa aaaaaagaaa aatccgctta tgcaacttct tctttttgta ttttgaggcg
     1201 ctaccccaat ccccaagtat cggttattta atagcgcgga aatccaaaga ataggacgta
     1261 ccctgaaaca aaaaaagact gctctcacga gcagcccaaa atgagattat cctattcatc
     1321 aacataaaaa tattacaaag tgaagaacta gagaaagaca aaaaaagcat atcaaatcct
     1381 ctcaaaaata acaagtcttt aaaaaatgac agataaaaat gcacaaagaa ctaaaaaaga
     1441 gaaatcgcgt aaaatcaagc attagaagct agtaactagc tagaccaatt aaaaatcaca
     1501 aagtgagcgt ctctatccat tggtagaggg gctacttggc atagtgtaca ttatgttaac
     1561 taacgctata aataacgatt atgttcccaa aacaatattt tatgaactat aacaaaaatt
     1621 tgttatcgca tattgtatat tttaattata ggtgtatacc acttaaattt aacacaaagg
     1681 cgtgattatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
     1741 tggaattttt agtataggaa ttacaaatac ctatgcttca gaaaattcta atttcaatat
     1801 caactcccaa ccaattgaag aacaagcaat taattttgcg aaaactgatt ttgaacactc
     1861 cataacttca attcttttaa tggaaaataa caccgaaact gatattaact atactcttgg
     1921 ttcgccattt aaaatgctcg atggagaaaa tacggcaaat agttctattt tctttccagt
     1981 attttcagac gctgataata cgattaaata tatttatacc gtcaaaaaaa atacaaatgg
     2041 agaatattca ggaagtattt cacaattttt agcaaaagaa cttcaaaatc tagtagatag
     2101 taatataaca gatacaacct tattttttca agataatagc atttattatt ccgataatgg
     2161 acaaattaaa ctactttgga aatcaccttt aagtacagaa aaaaatggtg tttcttcctt
     2221 tacagaaaaa aatgatctta tatccgtaaa tataaaaaat aaaattaaaa atttctctaa
     2281 aaaacaaaat tttcctgcgg ttaaacgtgt aaaaagtgtc tcctccggag ataactttat
     2341 tatgattaat tggcgtatag cagaaaccca aacaagtttt ccgtggtgtg ctgcttacgt
     2401 agaagctgct atcttatgta acaaaacaga tgtacgtgct acttcagcta gagccattat
     2461 gcattatacc tatcctaatt taaacaacag tcaaattgaa tcaaaaagtc ttaatcaaga
     2521 tcaaataata aaatatgcaa attactgtgg tgtttatcct aaaaaagtag cacgaatact
     2581 aaacctttca gaggtccaaa cacaactaag aaaaggaaat gccatttata tgggaactgc
     2641 tggttatgga aattatagta acagccgtca tgcacttgca ttatttgggt gggtcaatca
     2701 aggtggttat caaacttact atgtgtggaa tccttggtat aactacccaa ctgtaataag
     2761 tgcaaatgca ggaccagtta caattcctgt acctggaggt ggttttactt gggacaatac
     2821 cattacaaat tggtaaataa acccattgct ttagttatgg gtctagtgtt agttcttttc
     2881 ttaactagtt gttcaaaaaa acaagaaata caaaagactg atctgattcc ttttctagga
     2941 acttggcaaa ctaaagatca ttcagaacgt ctagtaatca ctaatttaaa tacttcaaaa
     3001 aatatagaac aatataccat cttagtaaaa gagaacaatc ataaaattca aatcattgat
     3061 aaaaatttaa atataaatga acaagttttc atttacaaag caacaaataa tttactaaca
     3121 ctgcgctcta ataaaaaaca attagattac acttttagtt tggattctcc aaaaaagctc
     3181 gtatttattt tgacaacgaa tgatgggaca gaagggacag ctaaaccaat tgaatttttt
     3241 aaagctgatt gatgaatagt tgtttgatat tacaaaacaa taatttcacc gaaggtgaaa
     3301 caagcgaaac tttacaaaaa gtggctagca tttgctagcc ttttttgtat tgtggagcgt
     3361 gggttttgag ggggattcaa gggggagctt gcgccccttt gaaaagcggt tatttgcacc
     3421 aaatacgtag gtatttggct agcaaatata gagcttgcca aacactttag tgagttttgt
     3481 agaaagtacc tttaaaggta ctttcacatt acgaacgata cgtccgtagg acgtactgaa
     3541 agggtgggat ttatgagcga actaatgcaa aatttggcta gcaaaaaaag ttaaaaaata
     3601 tatgctaata atcgagcgaa atgaacactt tttcaagtga gcgaatccgc catgacaaag
     3661 ctgaagcagt ttatttcagc tttgtcatgg cgtgtgccta gacttttaaa aaagtcggca
     3721 cacgagcaaa tatctttatc aagctctaat ttgcgtttta agacgtttga aacgtctaag
     3781 tatataaata tataagagta ggtttaagag tgcttaaatc gaattttaga gggcattcag
     3841 acaactttag ggttgttagg gcttgccctg accgcctgta aggcgcttta agaacaagat
     3901 aagggattta tactaagact gtcttaaaac gtctcaaacg aaggctcata gcttctcaaa
     3961 gagttcctta tgaacatttt tagcaaagga gccggaagcg actgcgcagt ataatgtgag
     4021 agcagagcga tcacaccttt ttaagcgact gtagggagta agattcccag ttgagaaaaa
     4081 tgcgcgaatc tgtttggaga aaaaagaagt ggttttttag gcgggaaatt ttgtccccct
     4141 cttttataat ctctttttaa acctcttttt aaacctcttt accgtcggct atatccttac
     4201 tctcccaagg gaatgacgct tttatctaca tcgttttgtc tgtttatcta catcgttttg
     4261 tctgtttatc tacatcgttt tgtctgttta tctacatcgt tttgtctgtt tgtacattga
     4321 attagtacat cgacgatgtt aatataagat gtagataaac aggcattttg tatttcataa
     4381 cgtgtatctc ttgtgcgagt aagaaaggag ctattttaaa tatgtctaat gaaatcgtaa
     4441 agtatcatca tgaattaaat acaattccgt tgcgaaaatt tacaccagtc gaaatgaatt
     4501 tgttcttttc tatcgtatca agaatgcgtg atgtaggtga taaaaaagta caatttacat
     4561 tcgagcaatt aaaagattta agcaattata aagcaactgc aaatgttcga ttcatagatg
     4621 atttagaaac aacgtatgac aaattaatgg atttacgttt tggtagaaga agtgctgacg
     4681 gtttgcaacg tgaacgtttt gttttattta atcaatttaa aatcgacgga aaatctgata
     4741 ttccttttgc tgaaatacaa gttcatgaaa aagccttacc cttacttaat aatcttgaag
     4801 aatgggtgcg ctattctctc caacaattca atgaattaga aagcagttat tcaaaaacaa
     4861 tgtttcgatt actaaaacaa tacagaacca aaggtttcgc ttatttttca aaagaagact
     4921 ttcatgaatt acttgatatt cctaaaagtt acaaccaagg tacgattgat caacgtgtgt
     4981 tcaatccaat acgacaagaa ctaaccgcaa tctttaaagg tttaacaatc aaaaagaaat
     5041 acggaaaagg acggggaaag cctgtcattg gctatcaatt tacatttaaa cctgagataa
     5101 aaaaatcaga cgactttaac aaacatgccc caacaaaaga agaacaaaac aaagcattag
     5161 aactagctaa ccaacaagtt caatctgaaa aagcacctac ttcattacaa gaaaaaatca
     5221 aagaacaaaa gcgaaaattg gaacttttac aacagttaga aaagttagaa agagaacaaa
     5281 atacccaaaa taaagaaaat aatgcacaaa aatcagagat aaattccgat attccttcag
     5341 aactaaaaaa agacttatta gacaatcttc aaaacttatt caaagattga ggggttttta
     5401 gattttatga cagaaaaaac aatcaaagaa cttgctgaag aattaggagt tagtaaaaca
     5461 gcaattaaca aaaaagtttc tgacgaaaat cgaaaacttt ggttttcgaa aatcggaaac
     5521 aagtttgtga ttaatgaaac cggacaaaag gctataaaat caatgttttt taacgaaaac
     5581 gaaaactcaa atcaacaact agttggcgaa aaaacgaaaa ccgaaaacca caaaaacgcc
     5641 aaccaagttc ccgaattaga agtcctttct ctcctagaac aacagttaaa tcaaaaagat
     5701 atgcaactac aagaaaaaga cgagcaaata aaacaaatgc aaaaattact cgaccaccaa
     5761 caacaactca ccctgcaagc caaccaacaa atcgagaaac tgcaggaaca actacagttg
     5821 acttatacgg aagaatcttc ggaaaataaa tcaacgactc tttctgaaga aacagaatcg
     5881 atagaaaaac aatcaaaggc ggagaaaaaa tggtggcaat tctggcaata aaaccagcaa
     5941 tttaacagaa ttaggcagaa gtcgcccatc ctctataggt gggtgatgaa tgccgttcgg
     6001 tatgagggtt accgttggta actcgaagac cgacaactaa ggcgtattgc ctttttattt
     6061 tgtcccatgt tacaatcagt ctatacaaga aatgagttga tataacatgg aattagatac
     6121 aaataatcat tcagtatttc ttctttatta ccaccttgtt ttagtaacga aatatcgtag
     6181 acaagtgatt gatgatgaaa tatctgatta tgccaaaact acttttgaaa gaatttcaga
     6241 atcgtatcac attactttag tggagtggaa tcatgataaa gaccacgttc atattatgtt
     6301 caaagctcaa cctaaaacag aattgacaaa attcatcaat gcctataaaa gtgccagctc
     6361 aagattgata aaacgtgact ttccaagagt caaacaattt ctttggaa
//
LOCUS       DAGCYL010000152         6330 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.154, whole genome shotgun sequence.
ACCESSION   DAGCYL010000152 DAGCYL010000000
VERSION     DAGCYL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.154"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(105..293)
                     /locus_tag="MB216_002271"
     CDS             complement(105..293)
                     /locus_tag="MB216_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBT4527062.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            563..925
                     /locus_tag="MB216_002272"
     CDS             563..925
                     /locus_tag="MB216_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBT4527063.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            977..2659
                     /locus_tag="MB216_002273"
     CDS             977..2659
                     /locus_tag="MB216_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBT4527064.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2776..4812
                     /gene="recG"
                     /locus_tag="MB216_002274"
     CDS             2776..4812
                     /gene="recG"
                     /locus_tag="MB216_002274"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBT4527065.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4861..5862
                     /gene="plsX"
                     /locus_tag="MB216_002275"
     CDS             4861..5862
                     /gene="plsX"
                     /locus_tag="MB216_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBT4527066.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5984..6226
                     /gene="acpP"
                     /locus_tag="MB216_002276"
     CDS             5984..6226
                     /gene="acpP"
                     /locus_tag="MB216_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBT4527067.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2112 a         1101 c         1437 g         1680 t
ORIGIN      
        1 caaaaataaa cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta
       61 aataaaaaac cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact
      121 tttcctgatt tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata
      181 acgcgaactt tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa
      241 cggttgtttc cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt
      301 tcctcctttg ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc
      361 atatagaatg ggaatttgca agtacatttt catagataca attgacaatt gagaagaata
      421 attgtaaatt aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa
      481 ctgcctttta tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt
      541 tacccgttaa ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga
      601 aattaccaat gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt
      661 tgtaggaatg gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga
      721 aaattactca agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta
      781 tacgattatc agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt
      841 gaaatacaat ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca
      901 aggtgttcgt gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg
      961 aaggaggatt atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt
     1021 ccaggcaggt gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt
     1081 ccctgtgcca gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa
     1141 ggcagtagta gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg
     1201 attgctgatg ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt
     1261 ttctaagcaa attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac
     1321 tcatggagtt gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac
     1381 agtagctaga gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt
     1441 tgaagtaatg acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt
     1501 gcttccagtc ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt
     1561 ttatgagggc tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc
     1621 ttctccagca gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca
     1681 aatggcgaca gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga
     1741 aggacctaca gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat
     1801 aggagattct ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga
     1861 aaacccagga gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt
     1921 cgacaacatg cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt
     1981 tgcgaaaaaa gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg
     2041 cgtccaagag ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat
     2101 gaatccaagt acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat
     2161 tgtcttgcca aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga
     2221 tatcccagta gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc
     2281 attcaatgat caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag
     2341 cgttgtgagc ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat
     2401 caaagaagga gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat
     2461 cacagcaaca gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac
     2521 gatcttggca ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac
     2581 agaaaaacat cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta
     2641 tctattttct gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat
     2701 aaaaggcctt cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg
     2761 agggaggaaa aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga
     2821 gaaaaaagag cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact
     2881 tattatcctt ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa
     2941 gaaaaggtta ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat
     3001 aaaaaaagcc gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt
     3061 tttaatcaac cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt
     3121 aaatgggatg caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg
     3181 gatgactttt cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat
     3241 ctaatcaaaa aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac
     3301 cttgtggaga aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca
     3361 aaagaccacg aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta
     3421 tttcaaatgc gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag
     3481 atcctttatg atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca
     3541 aatgcacaaa aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg
     3601 cagcgacttt tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta
     3661 tacgctaccg ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca
     3721 caacaacata tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg
     3781 attacggggt caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg
     3841 gaaattaacg tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat
     3901 ttaggtctag ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt
     3961 cgtgaaaaag gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca
     4021 ttagctatta cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga
     4081 cgtatcccga tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg
     4141 acgaaaaaag aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa
     4201 tcagaagcat tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt
     4261 gcaccaaaat atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta
     4321 atcatggaag ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa
     4381 gtaggggtaa acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga
     4441 ttggcacagc tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt
     4501 atcttagttg ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag
     4561 accaccaatg gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta
     4621 tttggcttcc gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga
     4681 aatgttttgg aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag
     4741 atgcttcctg agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt
     4801 tatttcgatt agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca
     4861 atgaaaattg ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc
     4921 gtggctttag cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca
     4981 gaaatcaaga aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa
     5041 atcaatagtg atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc
     5101 cttgctgcac aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca
     5161 ggcgcgctac tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct
     5221 ggcttgatgt ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat
     5281 cttggcgcta acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct
     5341 ttttatgcac gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga
     5401 acagaagcaa caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa
     5461 acaagcttga attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat
     5521 gtagtggtca cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca
     5581 atgaatatga tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg
     5641 ggcgctatgc ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa
     5701 catggcggtg ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca
     5761 ggccccgatg ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta
     5821 gttggacaat tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc
     5881 gatccgttcc attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg
     5941 gtgtaggtaa ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt
     6001 atttaacaaa gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac
     6061 ggatgaattg aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt
     6121 tttagagctt gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga
     6181 aacagtcggt ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc
     6241 aaagaaggat aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac
     6301 gtaaaagtca tttttctgat aattctggcg
//
LOCUS       DAGCYL010000153         6299 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.155, whole genome shotgun sequence.
ACCESSION   DAGCYL010000153 DAGCYL010000000
VERSION     DAGCYL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.155"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(21..644)
                     /locus_tag="MB216_002277"
     CDS             complement(21..644)
                     /locus_tag="MB216_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527068.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(803..2200)
                     /locus_tag="MB216_002278"
     CDS             complement(803..2200)
                     /locus_tag="MB216_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4527069.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2232..3203)
                     /locus_tag="MB216_002279"
     CDS             complement(2232..3203)
                     /locus_tag="MB216_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4527070.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3577..4002
                     /locus_tag="MB216_002280"
     CDS             3577..4002
                     /locus_tag="MB216_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527071.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4490..5191)
                     /locus_tag="MB216_002281"
     CDS             complement(4490..5191)
                     /locus_tag="MB216_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBT4527072.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5207..5647)
                     /locus_tag="MB216_002282"
     CDS             complement(5207..5647)
                     /locus_tag="MB216_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBT4527073.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5647..6222)
                     /locus_tag="MB216_002283"
     CDS             complement(5647..6222)
                     /locus_tag="MB216_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBT4527074.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         1790 a         1309 c         1001 g         2199 t
ORIGIN      
        1 gatttcacaa cctctgtttt tcattgtcca taggattgat attgtccttg tgatgactca
       61 ttcgtaataa ttgaggcttg attttcttca cttcccgtta tcgtttgtcc actgccagaa
      121 tctgaaggac ttgttcctgt cgactcgttt ttttgagaag agacagttgt tttcttgact
      181 tgttcatttg tctttttcca ttccgctaaa cgatcggcga ccactttttt gtctactttt
      241 tcagaataag cggtgatcac ttttccattt ttcaaacggg ctactttgtc gccatcatga
      301 gtcaaaagca aaacggtcaa atgcttgtct ttaaattgat attgtctgag agaaatcgca
      361 ctaagtacgt catcgatatt aataaattga ttttcttctt ttccttttgg caaataaatg
      421 tccaattcgc ctttttcgta attggcagtt attttatact ccgcccacca ataagttgag
      481 aattcttttt tgaaattatc tactgattct ttgcttgcta cgattttttc tgtttctttc
      541 tgagcaagat ctgctgtcat ttttcctgaa aatgctgcgg ataaaataag cagtaagaaa
      601 aaaccgaata accaaaagag aagaaacggt ctttttttcc tcatgctttc ctccactcct
      661 atttaatttt gactattcta ttatatacaa aaaatgaaat agaaagacaa aaaagttgaa
      721 aaagatcgta aataattgaa cttctttttc acaaaatggt aagaatggag aatttcacgc
      781 atttgctctc aactattctt tcttaagcct caaatccgtt tttatctgat acgtctttga
      841 accaatgacc cgattttttg attgttcttt ttccatcttg tgctaagtct actgaaataa
      901 agccatatcg attcttatat gcattggtcc acgaccagtt atccatacat gtccacatat
      961 gatagcctaa acaattggca ccttcttgaa tggcttgatg gacatatttc agatgagatt
     1021 ggacaaattc gatccgataa tcatcgtgga tcatgccatc ttctttgata aatcgtgctt
     1081 cgttttctac acccataccg ttttcagaaa taaagcagtc gatatttcca taatgatctc
     1141 gtacattgat caaaatatca tagattcctt gttcatagat ctcccatccg cgataaggat
     1201 tcattttacg accaggcatt tcataattgt cgaaataatc ctcaggcatt ggaccgtttt
     1261 tagtatcgat tggtgtttct tttgctttta cccggcgtgg ctggtaataa ttgataccta
     1321 aaagatctac tgtattttct ttgataaccg tcaaatcctc tgcatggatc acaggcatga
     1381 gatcaagttc ttttactatc tcaatcaatt cacttggaaa ctcgcctttt acagctggat
     1441 caaggaatga acgattgaag aacgcatctg ctatttgcgc tgctttaaca tcttcagggt
     1501 ttgattcgtc tcttggatag cttggtgtca gattcaagat gataccaata acaccacctt
     1561 gctccatttc atggtaagat ttgatggcta acgctgaagc caacgcttca tgatagccaa
     1621 cttggatcgc ttttttcata tctgactctt ctggataatg ccatcgatat aaataacctg
     1681 cttcgactgg aacgattggt tcattatgag taaaccattt ttttactttc gaaccaaaca
     1741 attcaaaaca acttttggca tatttttcat aggcttcaac cgtttctcgg ttcaaccatc
     1801 cgcctttttc ttgcagatac atcggcatat cgaaatgata cagattcaca aagggttcga
     1861 taccgttttc aatcaactca tcaaaataac gatgatagaa acgaacggct tcttcgttga
     1921 ttgttccaga ttcatcaggt atcaaacggc tccattggat agatgttcga aatgaattat
     1981 gcccagtctg tttcatcaac tgtacatcct cttgatactt cgtataaact tgcgaggttt
     2041 tttcaggtcc tacttgatca aagaactttt gcggttcttt tttgtaccaa taatcaaaaa
     2101 tgttctctcc ttttccatcg cctgaaatcc ttccttctgt ttgtgggcca cttgctgcag
     2161 aaccccacca aaaatttgct ggaaattgat attttttcat caaagcacct ctttttcttt
     2221 tttagttcgt tttagttgat tccatgtatg atattttcca tcacaatcac tttttcattt
     2281 tcttcctctt cttttttgat tcgttttaac agctgttctg ctgctcgttc cccgatttta
     2341 tctgttggtg gcgtgatcgt gattttcgga aggatactca gatcgttttt atctaaataa
     2401 tcatagtgac tgaacgcaat atccttcccg attctgacac cttcttgatt gaaacattcg
     2461 atggcaccaa tcgacatatt gtaattgctg acaaaaatag cagttgcccc tttttctaat
     2521 aatgcttttg ccccgtaata gccgcttttt ctagaatagt ctcctgtaaa gacctctaat
     2581 tggatcgatg gatcattttg gaccgcttct ttggctcctg ctaaacgttc tcttgctacg
     2641 tagtctgttt gagtggcagc aataaatccg atttttttat gtccttgttc accatgatga
     2701 gcgatcactt ttgctacact ttctcgatca ttcactaata ttgtatcaat acctgctggt
     2761 ccttctggac aattcaatgc gataactgga atactcattt ttgtgagcat ttcaaatcca
     2821 gaccactctt cttcggataa gaagacaatc agcccatcga tcacatgctt catcaggtat
     2881 tcaatttttt gttcaatttt ttctggctga tcggcaaacc cgcttaataa taagctatac
     2941 ccattttctt cagctactct ttcgatccct gagacaactt ccgacccaaa acggctggaa
     3001 atcccgttta tcaaaagtcc aatagagaag cttcgattat tcttcaaccc tttagcaatg
     3061 atgttttctt tatagtccaa cgctgaaatc gcttctgcta tttttttcat gttcttttcc
     3121 ttcagttgct gaccattcaa gtaacgggaa actgttccca ctgaaacacc agccatctta
     3181 gccacatcac ggatcgatgc catacattct cactcctgcc agttacattt gatttctctt
     3241 tcttgttgtt cgattatgat gaccgcattc tttgcatagt gttgcttgtc atcaatatcc
     3301 atataactaa agtcttcagt agagcaactt cctctcacta ttaattatac ttaaacaagt
     3361 atttgaaccg gttcattttt aatatagcat ttttttaaaa gattgcaaca cttttttaaa
     3421 acgctttctg aaagtggcgg attttttgcc tttttaaaat tttattctaa aatttactct
     3481 atttgtgggt gttttgtgcg tttttctctt ccatccgttt gaagttttac attccaatgg
     3541 tataatttct ttatcctatc aaatggggga agtattttga agaaattaat cgtactattt
     3601 attttgtctg cgggactttt gacattgact ggctgtgatt tgtttggcgg ctctgaccaa
     3661 tcctcggacg aaacaaccga ttcttctgtc ttaaaagcgg aaaaagcaga taccaccaac
     3721 agtgtatata cgctaaaagt acgtaaagat atggatttat ccgcgggaca gttgacattt
     3781 acagataaaa aaatggaatg gacacgcacg tatcagacac aaggaactga ttcttctgaa
     3841 tcatctgaag aaaaaacagt gctgacagat atcaagatca ctaccaaaga tgacgaatat
     3901 attatttcag gaaaagaaaa taaaaaagaa acaacagtca tatttacaaa aatcggtaat
     3961 taccgtttga aggatgagga tggcaacatc tacagtctat agtgccgtct gttggatctt
     4021 cttttgtttc tccccttctt ttctgctata gtaaagaagc aagatgattg taaaggagac
     4081 gaaatggatg atcgatagtc ctgattattg tacatgtaat aacaacaaaa atatccatac
     4141 agaatttaat gaccaaggaa gcttgagcat atgcgacaac tgtcacaaac tagtcaaaga
     4201 ttcatttaaa acatttgaat cttatcctgt tcaaaattat gtaaaaagta gaagaagata
     4261 aacaaatcca agcaaaactc actatggctg atttttatga acttcaaact cagcttcttc
     4321 ctttgatcaa gcttcatcag taaaacaaac agtggatcat ttcagtttac aaatcaaaga
     4381 gtaaagattt atttatttca aatatttgaa tataaaataa ccaaacgata gaaaggctta
     4441 ttctacatca cgtagtaatt tttctttccc gtcgtttggt tttttatttt tagcctaatt
     4501 cttgaccttt tgcatgctgg ttatccgtaa aatattttcc agcaggcttt ccttccaatt
     4561 cagctaattt ttcttggatc tgacggatca ccttttttgc atcttctgga ttttctacaa
     4621 aatcgaattt atccccatcc acttgtattt taggacatac atcaaaatct tcataccatt
     4681 gattatagcg ggtgttcaac gtttcataat aggtatataa cgtctcatcg ctttctagct
     4741 gttcatagtc acgcccacgt tttttgatac gttctagcat cgtgtccaaa gaaactttga
     4801 tatggaccat caaatcaggc cgtttttttg gagcaacatc atctaactcg tttagcatcg
     4861 tatctaataa gctatcgtac tgctgtactt cgatatccgt tacccgacct aaatcagcgt
     4921 ttagatggaa caacaggcta tcttcataga tggatcggtc tagcacattg tcatcgttca
     4981 ctaaagcatt tttcattgct aaaaatcgct tgttcaaaaa gaagatttgc aataaaaaag
     5041 cgtactgctc aggatttgaa taaaagagcg gcaatacttc gttatcttcg acattttcat
     5101 aaaatggttt agaatcaatc tcttttgcaa gcatatccgt caaactgctc ttacctgcac
     5161 caatcgttcc tgaaatcaaa atcactgaca tacaaccctt cctcttctat aagtactcgc
     5221 ctacgtaagt ttcaacaggc aatgtttcaa aatcatattc acgtacagct tggtctgatt
     5281 ttaaatacgt atggagactt tgtgtaatca tcaagttcgt aggtaccgtc tcttcttgta
     5341 caatgatcat cggttttttc aacatagttg ccacacctaa ttcgtaagcc gttcctggat
     5401 cgattgtttg atgctcaaag tcaaggatag caacaacaaa ttctgcattt gtcagttctt
     5461 ccatatcttt ttcgtatact tcctgcgccc atccagcgct gaatagttca taattgcttt
     5521 cttggtgatg acgtggagag tagaaacttg tgactgtttt gttttcctcc aacgcttttt
     5581 cgatacgact tactcgatcg atttgctcct ctgaaaaaaa tggtcctgct aaatatattt
     5641 gactcattat tttgcctcct tcaataataa ctgattttta atagatactg ctcgtaattc
     5701 attgaccagt tccaatggat tgttggaaaa gattacttga tccaaccatt ttttatccat
     5761 aaaatcctct tttgcaaaag tttctaataa ttgttgcaag ccgctgtaat aaccgccaat
     5821 attcaatatt ccgattggct tgtcgggcgt cagaccgatt gctatccaag aaagaagttg
     5881 tgagaattcc tccaatgtac ctagacctcc agggaaaacg aggacagcat ctcccttttc
     5941 aaataataat acctttcttt catcgatcgt ttctgtagga ataaatgtag taacatcttt
     6001 tccaggcaat tcagtttcaa aaagcccatt cggatagata cctgtgactt ttccgttatt
     6061 ttccagtact gttttactaa tcactcccat aatcccggag tatgaaccac cgtaaaccag
     6121 ttcgatttct tcttgtgcca tgtattttcc taacgtttgc gcaataaatt tgtaactttc
     6181 tttattacca aaacgggaac cgcaaaaaac agtaagtttc ataaaaaaac actccctaac
     6241 tcaaaagtct atcttcattc tacacgtgtt tttttaaaat aaaagactag attttgtag
//
LOCUS       DAGCYL010000154         6271 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.156, whole genome shotgun sequence.
ACCESSION   DAGCYL010000154 DAGCYL010000000
VERSION     DAGCYL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.156"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            41..418
                     /locus_tag="MB216_002284"
     CDS             41..418
                     /locus_tag="MB216_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4527075.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            406..744
                     /locus_tag="MB216_002285"
     CDS             406..744
                     /locus_tag="MB216_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4527076.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            878..1321
                     /locus_tag="MB216_002286"
     CDS             878..1321
                     /locus_tag="MB216_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBT4527077.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            1583..2398
                     /locus_tag="MB216_002287"
     CDS             1583..2398
                     /locus_tag="MB216_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4527078.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            2408..3070
                     /locus_tag="MB216_002288"
     CDS             2408..3070
                     /locus_tag="MB216_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBT4527079.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            3275..4939
                     /gene="alsS"
                     /locus_tag="MB216_002289"
     CDS             3275..4939
                     /gene="alsS"
                     /locus_tag="MB216_002289"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBT4527080.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            4952..5662
                     /gene="budA"
                     /locus_tag="MB216_002290"
     CDS             4952..5662
                     /gene="budA"
                     /locus_tag="MB216_002290"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBT4527081.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            5792..>6271
                     /locus_tag="MB216_002291"
     CDS             5792..>6271
                     /locus_tag="MB216_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBT4527082.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     "
BASE COUNT         2041 a          990 c         1343 g         1897 t
ORIGIN      
        1 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
       61 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
      121 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
      181 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
      241 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
      301 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
      361 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
      421 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
      481 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
      541 caactgaagg agttagaaga ggaactagga acagaactgt ttgtacgcga aaaccgcaaa
      601 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
      661 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
      721 tacgatcggc tgtgtggaag ctgataattc gaatacattg gcactattac tagaaaagtt
      781 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
      841 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
      901 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
      961 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
     1021 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
     1081 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
     1141 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catctttcaa agcgagtgac
     1201 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
     1261 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
     1321 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
     1381 ttgtattttt tcatttttaa atcaaaaaaa ataaagagat ttacataact tgttaaataa
     1441 gcgcttacaa acataagaag aacggaaatg aatcagctat aataaccgtt gttctggatt
     1501 gtaatcatta caaagaaatg aagattataa ctttggaggt gttcgaaatg aaagaaaaaa
     1561 agcaaaaatt cgttcaaaaa acatgccctt tattcaatgg ttgggtatga ttctgctgat
     1621 tgtattagct ttattgacga tcattccttt tttatggatg gtttctgctt cattcaaagc
     1681 aaataatgaa gtgttcacga ttccgattca atggatccct aaaacttggc atttagagaa
     1741 ttatctggtt atttggtctc gtattccttt agtgacattt tttaagaata cagctttttt
     1801 aagcgttgtg atcacgttga ttcagttgtt tacttctagt tttgctgctt acgggttttc
     1861 taaactaaat tttaaagggc gcgatatttt atttttagca tatatcggca cgattgctgt
     1921 cccttggcag tcttatatga tcccgcagtt cattatgatg agaaaagtgg gtttaacata
     1981 tacgttgtgg tcgttggtgc tattacaagc gttcaatgct tttggtgtgt tcttgttgaa
     2041 gcaatactac agtagtatcc cagattcttt atgtgagagt gcgcggatcg atggcttatc
     2101 agagtgggga atctatcgca agatcatttt acctcttaca aaaccggcga ttgccagttt
     2161 gacgatcatc acattcgtta atacatggaa cgattatatg gggccattca tttatttgtc
     2221 ttctacggaa aataaaacaa tccagttagg gttgaaaatg tttattgggc tatttgatgc
     2281 agagtacgca ttaatcatgg ctgcctcagt tgtttctgtt cttcctgttg cttttatatt
     2341 tttagcgatg caaaaatatt ttgtagaagg gatcgcttca tcaggaatga aggaatagga
     2401 ggtgattatg aaagagcagc gttttgacaa aaatgtttat atggaattct tttattggtt
     2461 gtatcaatca ttggcaagta gcctttgttt ttggtgtgtc aatatcttgt atatagcagc
     2521 tgcgttcctt ttgtcgatag atggaaggaa tgtcttttcc tttatttttt ctttactttt
     2581 tattggtcca ggttgcatcg tgatgatagg tatgacagaa gaattagcaa aagaaaaaag
     2641 tttttcacca gtacgtttgt tcataaagac gtttcgccaa ttttggctaa agggattttt
     2701 ttattggcta tttgcttgga tagttagtgt gataatgatt tttgattgtt tcttttttat
     2761 acgtttttct tatggaaaat ggttgattcc gttgtttgtt ttacttgctt gtttatcagt
     2821 ttcctttagc atcaactact ggtattttca ggtgagaaat ccagcgtcga aaccaaatca
     2881 agttctacgg atagcgttct actatacgtt gaaaaaatgg tatgtgagtt tactagattt
     2941 tttactattg acaagtttgt ttctcttctt tttcgtaaag ccccaatggt gtattctact
     3001 aggtccttct atcgtatttg gtttgattta cttcaacaat cgtaaattga tgagaactat
     3061 ggatctgtag actttataaa ttgaaacttt caattaagaa tgacagtttt tatgtaaaaa
     3121 ctgtcattct ttttcatttt ttgaaacata tggctaaaca aatgaaataa aacgtgctat
     3181 acttttgaat gaaacaggtt tagaaactgt atcaattatt tgtgattatt tctaactaat
     3241 aattaaaaat aaagcataaa aaggacgatg ttcaatggac gaaaaaatga aacaaggttc
     3301 gaaatctgta gtagaaagtt tgattaatca tcatgttgat tatgtattcg gtataccagg
     3361 tgcaaaaatc gatggtgtat ttaatgaatt agaggatcaa ggtcctgaat taatcgtgac
     3421 tcgtcacgaa caaaatgctg catttatggc acaagcaatc ggacgtatta caggagagcc
     3481 aggagtagtt atcgcaacaa gtggtcctgg tgctagtaac ttagctacag gtttggtaac
     3541 agcgacagca gaaggtgacc cagttttagc aattgccgga caagtaaaac gaagcgatct
     3601 attgaagttg acccatcaaa gcatggataa tgcagcactg ttccagccaa tcacaaaata
     3661 cagtgcagaa atccaagatc cagaaacaat ttcagaagtg attgccaatg cttatcgtat
     3721 ggctaagagt tcaaaaaaag gcgcgagctt tatcagtatc ccgcaagatg tagtggatgc
     3781 acctgtccaa gggaatgtca tcaagccatt gagcgatcct aaattaggtt ctgcttctgc
     3841 agatgatatc cgttacctag ctgaacgcat tagagaagca aaactaccgg tactattagt
     3901 aggtatgcga ggatcaagtg aaaaagaaac gttagccatc aggcaattag ttggaaagac
     3961 agcattgcct gttgtagaga cattccaagc agctggagtg atctcacgtg aattagaagc
     4021 gcatttcttt ggtcgtgtgg gtttatttag aaaccaacca ggagatatgc ttttgaaacg
     4081 aagtgatcta gttattgcta ttggctatga tccaatcgaa tatgaagcaa gaaactggaa
     4141 tgcggaaaaa gatgcgcgga tcattgtgat cgatgaagcg ccagctgaaa tcgatccttt
     4201 tatgcagcct gaaagagaat tgatcggtga tatctcagcc actcttgatt tattgacagg
     4261 ttcattagag cctcaacaag tgtcagaaga tgcaaaagaa tatttagcat ctcttcaagc
     4321 agaattaacg gagcgggaca ttgttcaatc aaaaggagaa gcaggtatct tgcatccttt
     4381 agaagttatt aacacgcttc agtcaaaagt aacagatgat atgactgtta ccgtagatgt
     4441 aggtagtcat tatatttgga tggcacgcca ctttagaagt tatgaacctc gtcatttgtt
     4501 attcagtaat ggtatgcaaa cgcttggtgt tgcattgcca tgggcgattt ctgctgcatt
     4561 ggtgcgtcca aatacacaaa tcgtatcagt ttccggcgat gggggatttt tattctctgc
     4621 ccaggattta gaaacggcgg tacgtaaaaa attgaatatc gttcatttga tttggaatga
     4681 tggacactac aatatggtag agttccaaga aaaaatgaaa tatcagcggg cgtcaggtgt
     4741 agactttggt ccagttgatt ttgtgaaata tgcagaagct tttggcgcta aaggaatccg
     4801 agctacaagt gtagaggaat tagaaaaagc tttagaagaa ggatttgcga cagaaggacc
     4861 cgtaattatt gatattccga ttgattaccg tgataatgag aaattgggag aaacaatctt
     4921 accagatcaa ttctattaag gaagtgtcga catgactgaa aaaatcttat accagcacgg
     4981 gacattagga gctttgatgg ctggcttgat ggatggaaca gaaacgattg ctcatatatt
     5041 agaaaaagga actctgggat tgggaacatt gcatgggctt gatggtgaag tcatcttttt
     5101 agatggcgta gcttatcaag gaagatcaga tggctcagtt gcccaattag atggaagtga
     5161 acttaccccc tatgcagcaa ttacagattt tacgccagat actagttttt ctgtctcaaa
     5221 aactgcagat ggagaacaat tgaaaaaaga catattagta agagaagccg gggaaaacct
     5281 atttttggca gtgaagataa ctggattatt caaaaatatg cacatccgta tcatgccgaa
     5341 gcaagaaaaa ccttacagaa gactagcaaa aatttcagag tctcaaccag aattccaaca
     5401 aagcaatgtc caaggaacat tagtcgggtt ctttacgcct gaattattcc aaggagttgc
     5461 tgcagcggga tttcacttgc attttatcga tgatacacat acatttggcg gacatgtaat
     5521 ggattttgaa gtggctgaag gaaaagttga gattagccgc atctcgtccc ttgtacagca
     5581 ttttccagtt gatgatccga cttttgttca cgaaacgatt gattatgctg atttagcagc
     5641 agaaatccaa caagcagaat agaaaaagac agcgagatgc gttcaaggtg attaacctga
     5701 cgtatctcgt ttttttgagg aggaaaccta gcgtttacaa aaaaactgag ctataatctt
     5761 ctccatagat caaaaaggag cggtttttat catgtggaca gaaaaacaga aaaaagatgc
     5821 atacgaaatt ttattattac agcaacaagg attattagaa gcagaagata actggttagc
     5881 aaatttagca aattcatctg cattgttgaa tgagactttg cctgagactg tctttgcagg
     5941 atattattta ttcgatggta atgaattgat tttaggaccg ttccaagggc gagtttcttg
     6001 cacacgtatc aagatgggaa aaggagtttg tggagaatca gctgaaaaac aagaaacatt
     6061 gatcgttgat gatgtcaaaa cccatgcaaa ttacatttcc tgcgattcag ccgcacaatc
     6121 agaaatcgta gtgccaatgg taaaagaagg taatttaatc ggtgttttag atatcgattg
     6181 cggagtaacg aaagggtatg atgaaatcga tcaagcattt ctagaaaaat atgtagatct
     6241 tttattagca aattttaata taaatgcttt t
//
LOCUS       DAGCYL010000155         6267 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.157, whole genome shotgun sequence.
ACCESSION   DAGCYL010000155 DAGCYL010000000
VERSION     DAGCYL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.157"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(11..616)
                     /gene="dhaL"
                     /locus_tag="MB216_002292"
     CDS             complement(11..616)
                     /gene="dhaL"
                     /locus_tag="MB216_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBT4527083.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(644..1654)
                     /gene="dhaK"
                     /locus_tag="MB216_002293"
     CDS             complement(644..1654)
                     /gene="dhaK"
                     /locus_tag="MB216_002293"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBT4527084.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1629..2075)
                     /gene="dhaM"
                     /locus_tag="MB216_002294"
     CDS             complement(1629..2075)
                     /gene="dhaM"
                     /locus_tag="MB216_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBT4527085.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            complement(2114..3073)
                     /locus_tag="MB216_002295"
     CDS             complement(2114..3073)
                     /locus_tag="MB216_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBT4527086.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            3243..3563
                     /locus_tag="MB216_002296"
     CDS             3243..3563
                     /locus_tag="MB216_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBT4527087.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(3675..4544)
                     /locus_tag="MB216_002297"
     CDS             complement(3675..4544)
                     /locus_tag="MB216_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBT4527088.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(4640..5944)
                     /locus_tag="MB216_002298"
     CDS             complement(4640..5944)
                     /locus_tag="MB216_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4527089.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(5941..6102)
                     /locus_tag="MB216_002299"
     CDS             complement(5941..6102)
                     /locus_tag="MB216_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527090.1"
                     /translation="MKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(6133..>6267)
                     /locus_tag="MB216_002300"
     CDS             complement(6133..>6267)
                     /locus_tag="MB216_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBT4527091.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT         1679 a         1344 c         1133 g         2111 t
ORIGIN      
        1 tttattgaac ttaaggttga ttgatcacat ctaaaagcgt tgaaaataaa atggcactag
       61 aagcagctcc tgggtcgata tgtccgattg cacgctctcc tagataggaa gctcgtcctt
      121 ttttagctac caagactttg gtgtgttctt ttgcctggtc aatgatttct tttgttaaat
      181 tccctgcctt caatgcctct gtgactggca gccaaacatc taacatcgtt ttatcgtcag
      241 cttctgcctt tcctcggctt tgaatacccg ctgttccctg ctcgatcact tcgcctaatt
      301 tttcttgaga atcaatagaa tcgacctctt ttgtcgcttt catcatgttc atgaatgcag
      361 atccgtataa cggaccagaa gccccaccga ctttcgatat catcgccata gagaatactt
      421 tgaatgtatc gctgattgtt tcaggatgat cggcttgaaa agcttcttca taagctgaaa
      481 cccctcttcc catgttattt ccatggtctc catcgccaat cggggtatct agatcactga
      541 gatattgttt gttctgattg attgcctctg caaattgcgt gagccattct tggatttctt
      601 gaacggttag tttcatatta tcaccctttt atcttgagat tttttaccat gaaatcgtct
      661 gtacattgct ttctaaagaa tctttccatt gtgtctccgt caagtcgatc agtgttagag
      721 aaagaccttg catatctagt gaggtcataa aattaccgac ttttcggaaa actacattga
      781 cgcctctttc ttccaacaga gctaaaacat cattcatgaa cacgaactgt tccatcaaag
      841 gcgtcccgcc cattccgttg actagtacac ctgcttcttt tggtaaattc gagtaatcat
      901 ctaaaatctt agtaacaagt tcctttgcca gtactttaga aggctgcatc ttttctcgac
      961 gatagcctgg ctcaccgtgg ataccaacac catactcgat ctcatcttct cctagttcga
     1021 atcctggttt ccctacttct ggtacagttg ctgctcgaag cgctagaccg atcgtcttag
     1081 ttgcctgtac gaccttttct cccagttctt tcaactcagc tagggaagca ccgtttcttg
     1141 ctgcatcgcc cacgattttg tgtacaagga cagtacctgc aactccgcgt tttccagcag
     1201 tatacgtact attttcaacg gcgatatcat catctacgat cacactttct acttcaatat
     1261 cgtccatagc agctaattcc tttgccatat cgaagttcaa tgcatcccct gtataattct
     1321 taacgatcag caaaactcct tttccactat cggcttcctt aatagccgtc tgaatctgat
     1381 ctggagttgg agaagtaaac acatcaccaa gcaccgctgc acttaacatc ccttccccta
     1441 caaacccggc atgggatggt tcatgtccgc ttcctccacc agaaaccaaa ccaacttgtt
     1501 ccgctttctg attcttggct acaacacggg aagattctac acgatgaaca agttctggat
     1561 agctttttac taatccttcc agcatttctt cgacaatatt gcttggatca ttcatgattt
     1621 ttttcaattt attcccatcc tttctttccg gcattttgtg cttctgttaa gatttgctga
     1681 gtatcatctg ttataccggc agtgatggct gctgcaaaag ctccttccac taaaggcgca
     1741 tccaccagat gataacgttg ctgctgctct tcttccaaca tgtcttttgc cagttccgag
     1801 ttcaataccg cgctaccgat atccgcaaaa acaaaataac tagctgcatc gcttgattca
     1861 ttgactgctt ctacgatttt catcgggtct gatccgatct cgccatttat tccgccgaga
     1921 gaaaaaattt gcacctcttc ggatttttgc atctgctcaa tcatttcttt aataccatct
     1981 gttacttttt gactatgaga aataagcaaa atactttttt tcacaagaat cttcctttct
     2041 tttacatagt tgctaacgat tggtttcgct ttcattaatg aacaattcaa gtatataata
     2101 aatttttcgt ttgtcaaatt aagctagaca aatcatcttt acctacgtaa ctcaaaaata
     2161 cttccaatgg tgtgcgatag tgtaatgatt ttctaggaat atgatttcgt tttgatgcca
     2221 ccgattggat aaaggtttcg gataccttat taaaatccat agctttatat aagccatctt
     2281 tacgtaataa gccattcgaa tgttcattta gtcctcgttg agaaggtgtt cctggatcag
     2341 cgaaataaat cgaaatatca ttggtattac taatcttttt ccaattcgag aactcttttc
     2401 cacaatcaaa tgtgatggat ttaaacaagt gtttcggtac agattgaaac cattgattca
     2461 gtctattctc aatatctata gcttgcctac cagcgggttt caaggtgatg atcactttag
     2521 ataatcgctc aactaacgtg atcaccgcac ttttatgttt tccaccgaca atggtgtcac
     2581 cttctaggtg accaaactca tgattgaact gtatataatc agttgttcta tcctgaatcg
     2641 aacggcgaaa agcttgtttt cctctttttt ctttatgtcc gtttggtttt cttttccctt
     2701 tcattggtaa atcgtgaggg tcaaaggttt tatctttaaa catccgatat agtgtacgta
     2761 ccgaacaagc aataggaaac tctgctcgac caataaggac atctggagtc cacccttgag
     2821 ccaccttttt ttgaatgtat tcttgttgcc catctggtaa cacaatcgga cgtcttccgc
     2881 agttacgttt gtttttctta tattgttcat agtagcttat tgcgcttcca ccagatttaa
     2941 gaaaacgata gacattataa atggtttgtc gagaacgttg taccagatgg gctacttttg
     3001 aaacagagtg tgattgattg aaataagctt ctatcattac gagttcagtt ggtgtaagat
     3061 gggtataggt catttgtact cactccttat aattctttcg acggaactat tttgagtgta
     3121 gcacaaatga cttttttagt tgtctagctt aattttacaa tcggcgattt ctaattttgg
     3181 gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc
     3241 gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga
     3301 tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc
     3361 gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag
     3421 aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg
     3481 taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg
     3541 gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg
     3601 gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc
     3661 ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca
     3721 cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc
     3781 tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag
     3841 gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct
     3901 aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg
     3961 tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct
     4021 gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca
     4081 agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt
     4141 gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg
     4201 tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt
     4261 tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct
     4321 tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt
     4381 tccgaggcac cgttttcagc accagaagag cttatggttg ttgaaggttc tgtttgagca
     4441 ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt
     4501 ggccagatta acggcttcat tctttttttc atcacttttc ccatccttac tactttttaa
     4561 ctaatttctt attataagca aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt
     4621 ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg gcttttccat gtcccgcttc
     4681 cataacctaa aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca
     4741 ctatccagaa caaaagcata tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc
     4801 gatcatctac aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga
     4861 tgaacacaaa actgaatgta aaagtaatat aaatctgttc gtagtttcct tgatatacat
     4921 aatgcccgat taatccagca aaaagaacta cggaaataca aaagaatatt gaccagacaa
     4981 tactcaatgt ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa ggatggagca
     5041 tcactttttt gaaattcgtc agccagactt ctgttccacc ttttgcccaa cgttttctgt
     5101 gtcgatatag catcttcaac gtcacaggta cttccatgaa aaagataatc tcaggagcaa
     5161 aaacagataa ccagtcattt aactgatgat cccaagcaat actgatgtct tctgtcgctc
     5221 gatcttgtct gaatccgccc acatcgatca aggcttcttt tcgatacatc gtatttgctc
     5281 cactgtacgc ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga
     5341 tacttgagaa ttcaaccgtt tgagattttg caatcaattt ggtacgattt tggacatcca
     5401 tatttgcagt gaccgcagca atatgtcttg caccagggcg aatgaaataa ttcacatacc
     5461 taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt
     5521 ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa
     5581 ccacacgaag attagcgtat tttttcatca gtctagctaa tatttctggt gtttggtccg
     5641 ttgaaccatc gtctgtcact aatacctcat agttgtgata attcaatttc gtcatcaaat
     5701 actcgattgt atcttcgatc acaatttctt cattatgggc tggcaccatg atcgtgatga
     5761 acggttcgac actcaaggga acatctaccc attctttttg cttatgtttg tataaaaaca
     5821 cataacacca gacaccaata aaccatacaa atccgcctaa aatcggataa gaaacgagaa
     5881 aaaaatacat aatagacaaa caagtatcta agaataaatt acctgtatgg gtgattgaga
     5941 tcatttcagt tccaccccat tttctttata caaatctgca atgaatgtcg tatctagatt
     6001 tttctcttcg gagacagaat aaaaacggac cgtttctctc tcttcttttg ttccaaaacg
     6061 ttccgtatac acttcttcaa gtaattcttg tctttttttc aatttttcat cttcatatta
     6121 ttatttgtca aattaagcga gacaaaactt tccatttacg taactcaaaa aaacttctaa
     6181 tggtgtttgg taatttagtg attttctagg gatatggttt cttttggacg caacggatga
     6241 gatgaatcct tgattgactt ggttgaa
//
LOCUS       DAGCYL010000156         6209 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.158, whole genome shotgun sequence.
ACCESSION   DAGCYL010000156 DAGCYL010000000
VERSION     DAGCYL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.158"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            31..888
                     /locus_tag="MB216_002301"
     CDS             31..888
                     /locus_tag="MB216_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBT4527092.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            908..1468
                     /locus_tag="MB216_002302"
     CDS             908..1468
                     /locus_tag="MB216_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HBT4527093.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            2441..3208
                     /locus_tag="MB216_002303"
     CDS             2441..3208
                     /locus_tag="MB216_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HBT4527094.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            3234..3779
                     /locus_tag="MB216_002304"
     CDS             3234..3779
                     /locus_tag="MB216_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527095.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            3780..4346
                     /locus_tag="MB216_002305"
     CDS             3780..4346
                     /locus_tag="MB216_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527096.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            4361..4609
                     /locus_tag="MB216_002306"
     CDS             4361..4609
                     /locus_tag="MB216_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527097.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4628..4867
                     /locus_tag="MB216_002307"
     CDS             4628..4867
                     /locus_tag="MB216_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527098.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            4884..5468
                     /locus_tag="MB216_002308"
     CDS             4884..5468
                     /locus_tag="MB216_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527099.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            5575..5856
                     /locus_tag="MB216_002309"
     CDS             5575..5856
                     /locus_tag="MB216_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBT4527100.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            5846..6187
                     /locus_tag="MB216_002310"
     CDS             5846..6187
                     /locus_tag="MB216_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBT4527101.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         2311 a          822 c         1290 g         1786 t
ORIGIN      
        1 ttgaagtatt atatttagat atcataattt atggaagaaa aattaattaa aaaggatgag
       61 attataatgg ggttactggt tccgagagct aatacttata ctataccttt aacgaaacgc
      121 caattaattg ctaaaagata tcaaaggatt actagagcca tcaatagaga gttttggaat
      181 tctgaaagtg atactgctca cagtttatac gtaggttctt atgggagagg aacggcaatt
      241 agtactagtg atatagatat aatagttgaa ttaccgatgg cagaatttga cagattcaaa
      301 aattatttat caaatggccc gtctaagtta ttacaagtta taaaaaacgc atttcaggaa
      361 atactaccaa atagtgatat tagagctgat ggacaagttg ttaaaataaa tttccatgat
      421 ggaatcaaat ttgaaatagt tccagctttt aatgaaaaag attattgggg tgagtcaaaa
      481 gggtttattt atccagattc aaacatggga gggaactgga aagctacaaa tcccaaaaaa
      541 gagcaagaag ccatgaaatt aaagaataca aaaagtaata atttattata tgctacgtgt
      601 aaacattttc gacatgtacg agatacagaa tttacgagtt atcatctttc aggaatagta
      661 attgacagtt tcgtatatga ggctatggga aattggaaat tcgttgaaaa taacagtggg
      721 ggtcaaaaca ttagttctgt atcttatgag acagctttat tagaatacta taactcacat
      781 aaagttatgg gcggtttaaa tttatactct cctggaagca atcaatttgt taattctgat
      841 agtagtatca tatgtttaga aaaagtatta aaaaaaatag ctttataaag ttttatggag
      901 gaacaacatg ggagacgaaa aaaagcgctt ggaagttcaa ttacgtgaag catatggacg
      961 agtaacgtat acttacacca ctcatttaaa gcaagccgat aatattattt ctgacaataa
     1021 aattttaagg tgtcttcaaa ttggtttgtc agcccttact actggagggg tacttagtac
     1081 cattattact gataatagtg agttaacctt tgttgctgga ttatgttcaa caatttctct
     1141 tggattaaac ttatacttta agaattttga tcttgctgaa gaagctaaaa atcatcaaat
     1201 tgttgccgat aaattatggt tgatcagaga agaatacgta agcttactaa cagatttaaa
     1261 tcgtttaagt gttgaagata ttgtgaaaaa aagagatcaa ttaatgaacg aaactcatga
     1321 agcatataaa aatgccccta aaacaaatag taaaagctat gaatcagctc agaaagcatt
     1381 aaaatctgaa gaagagcaat tttttacaga agaggaattg gataagatgt taccagagca
     1441 tcttagaaaa aagagttcta aatattgaac tcttttttta tgcttaaaat tctcttgtaa
     1501 tttttctatt catctatatt taagaagaaa tatgatgata gaaacatctc ctaaagccac
     1561 cgcaagccgt tctaagcata gcttagcccg acattgctag atatgctcat ctcaccatga
     1621 acgtttccta aagccaccgc aagccgtcct agccattgcc tagtccgaca tcgctagatt
     1681 agtgcatctc gccataaaag ttttctaaag ccaccgcaag ccgtcctagc cattgcctag
     1741 tccgacatcg ctagattagt gcatctcgcc ataaaagttt tctaaagcca ccgcaagccg
     1801 ttctaagcat agcttagccc gacattgcta gatatgctca tctcaccatg aacgtttcct
     1861 aaaaccaccg caaaccgtcc tagccatcgc ctagtgccta gtccgatttt gcttgatcgg
     1921 ttccatttct gtttatttag tgggtcaaca ccgtaatctt aaatagccat cgcctattta
     1981 agattactag gtctgctttg aaggtaaaaa gtctttcatt cttcaagact tttaggcttt
     2041 caagctacgc ctgaaagcat tgaaattcat cgctctttat ttggttttgc catttataat
     2101 tccttacgcc cattatcttt ttcgcctgtc ttgccttgtc agaagtgttt agagcttatc
     2161 tcatctttgg attaatacaa gtggcgtaaa tttttttcta aacgcaagcg ttacgaaaat
     2221 aatttactta aacacttgta ttaatccggc agataagaac gcattctaat cactcccgac
     2281 aaggcaagac aggcgaaaaa gataaataaa tgaacgaaag gaagaaacat aaaatgcaaa
     2341 accaaatagc gatgaagaaa aaaccacctc cctctaaaac aatccaaaca aaaaagaatt
     2401 ttgaaaaacc aaaaatcaaa aacagaaaga agcgttcaac atgagagata gaattttaga
     2461 agttaatcaa gaaatggtaa acaagttaac aaatatcagt gggaactatg aaaagtacga
     2521 tggtacttta cttgataatt atatttttca cgatacacaa aatatcagaa ttggcagaag
     2581 taaaccgaga gcgttcattc tcatcaaaga gaaatatttg aatgaatgga atagcaccta
     2641 tgaaatggtt atgactgata actttgaaaa ggtattggat ttcagaaatg ctttgttggg
     2701 ttctgatatg ggaaatagca gtcttgaatc tattgaagtt gaaaacaaga ccgagaatga
     2761 aggtgaagga ttgattaaga atgtcctgaa aatcgaacaa aaatatatca gtgaagatag
     2821 aagtttttat agagtcacaa gctcggttgg tctagcaaac tttgctacta acttaattgg
     2881 aaacaatgct agtgaatctt tgctagtgat gtgtctgaat acaaagaatg aggtcattgc
     2941 ttatagcgaa gttttcaaag gtggaattaa tcaatcagta gcgataccaa gagagatttt
     3001 tcaactagct ttgctaaaca attcagcaaa aatcgcagta tcacataatc atccgagcgg
     3061 atatgaattt ccaagtgaaa atgacaaaag ttttactacg aatttgaaaa acgcaggaaa
     3121 aacaattggt attgaattgt tagaccatat catcgtaaca agtaaaaaca caagctatta
     3181 ctcatttcaa gaaaatggtt ctttataaaa attagagaaa agaggaaaac aagatgggcg
     3241 aaagaacaca agcgttaatt acagtcaaat atggtaacag caaaacgaaa gtaagcgtgt
     3301 caattcatta tcagtgggga tatggtcgat tcatgttgat ggacgttttg aatcaaggaa
     3361 tatatttaat caataaagca tatgacttac gagatgaatc aagagaagaa ttagcaaaag
     3421 gaatctttga tagaagtaca ggagttatca attttgttac ctatgaatct ggagtgagtg
     3481 cttatgatga aacagggttt ggtgggcgct atgaacctta tcaagaaacg attgaagatg
     3541 tgatgaagtt ttcagataac aatgatggct atgctcattt agagatggtg ttcgatgaaa
     3601 atggatttag tctagatgat aaaaccagat tgcgattgtt tgattttgag ggtcaacaga
     3661 taggactaaa caagttttgt gaaagcggaa aagagtttgc tacgaaagat tttattaagg
     3721 gatacagatt attgctagaa aattatggaa taaacctaga agaagagagc gtgaaataag
     3781 tgtttgaaaa aagaaaagac cagttcccta atttagaagc gaaaagtcaa aaagtgattg
     3841 atggtgaaat gattgaatta ttccaaaaaa gttatggaac catagaaact accgcaagtt
     3901 gcaaatacta ctttcaagga aagagaaaca cgtttaaagg ctttgccgat tctgatgaag
     3961 aattggcaaa gaaaatcatt gattggcttg tcagcagtga acacgtagca agagaagata
     4021 ccgttagaac aacgattgag ggtgaagtcc taacaggtac tagatgtgtt tgttgtaatc
     4081 atgaatttgt agaagatgaa ggttgctacc gaatcaatga atatggtgat tttatttgcc
     4141 agagttgcca cgagatagaa tttccaaccg aaagaagttg gtttgaatac atgctacaag
     4201 attatgagat ggaaacagaa aatggtgaag tagttttttg taccaaagag gaattgagta
     4261 agagaagcga tgatgaaatt gaagattggt gtgtgtggtt agtagggaca gatgattgcg
     4321 ttgcatatta cacaacagca gaataggagt gtgaatcagc atggaaggaa tggattttat
     4381 agatcatgaa gatttgattg attttggtta tacatggaaa ggaatggtag gtattagcag
     4441 aagtcttgct aatgcctttt acgaaagaaa ttatgcagtc tatgtccttt atgatgacaa
     4501 tacagaaagc cttgttgatg aagagtataa attggattta gagaatgttt tatatgggat
     4561 agaaaaagaa gatttagcaa aatatatttt ttcatggttg ggtcaataaa cgaaagaggg
     4621 tagcaacatg acaaaattca aaagtaaagg tatgcaatgg tgttcttatt attatgttgg
     4681 gaatgatgaa gagcttttga gaaaagccaa acaaaaaggt tatccagata agatgttaaa
     4741 acagattggc tattacatgc cagaagagag aatagaaaaa gaatcttgta aggttctttt
     4801 tgatagtgaa cagctattag ttttgttaac tcaaaataaa ttgatttgtc aatttgaaaa
     4861 taattaatat ggggtgaaga accatgaatg agaataagat ggtattaagt gaatacgcaa
     4921 gattgagaac cattaattgg ttgaatggta tgcaaagtag tggcgctttt gatggatttg
     4981 atgaggagaa tatggacttt gaaagtttat cgaatgagga tattagatat tattttatcg
     5041 aatggtattt tacagaacca gatgaagagg tggaagatga accagaatta attttagaac
     5101 gtcaaagaat tttattcagt gaacaagata gtaagaggtt gtatggttta tatgacttac
     5161 aactgaaagg attaaaagcg cagtatgttg ctgatagtta cgatgatatg gtagaacagg
     5221 gggcagatag ggttgtcact tattcagaag atgatgaaga ggaatgggaa aactattcag
     5281 atgatgagat atgctttaat gatgggtata tgcctattga aatcaccgaa gaagaatatg
     5341 ataaagtgtc ggatagcgat gagattggta tgtgtacggt tgtaagtgga ttggatatag
     5401 atgtccaaag agtagaggtt gcataccaga agatgaaaga acgtataaaa agtaaagtag
     5461 aatcttgatt tgattggatt gtttgcaaag tagctaatta agagtataat ataatcataa
     5521 atgtagttaa tggatataat aatgagtata ttatactcaa aagaggtgaa cgaaatgaag
     5581 gtaaaaaatc caaaagaaaa aagcagaatt caagtcggaa tagacaagga tttaaaagaa
     5641 aacgcagaaa tgatattgga agagctaggg ttaaatccaa caacagcaat cacaatcctt
     5701 tataagcaag tggtcgctag aggtgagttt ccagtagaga taaagctaag cgaagaggaa
     5761 aagcaaggaa tcagattgca acagctaact atggatatgc cagttgatgt tctagataca
     5821 gatgaaaagt tagaagagtg gttcaatgaa gcctaatgag atagtaacgg tctatgttgc
     5881 atttacagat aaaaatggtg gtaaaagaag acctattttg gtggttagtg ataaagaaga
     5941 tagagtagaa ttttttggaa tcacaagtca atatgagaat aaatcagacc aaataaagag
     6001 agtgtatttt cctattgcag aatgggaaaa agtaggtcta aagaagcaat catggattga
     6061 cgtagggtct ttaaaggcta ttcccaaaag ccgagagaac atatcattta aaaaggttgg
     6121 tatgctatca gctattgata cgaaaaagct aaataggttt atcgagagat tacaaagaaa
     6181 agtctaacaa ggcttttctt tttttgttt
//
LOCUS       DAGCYL010000157         6173 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.159, whole genome shotgun sequence.
ACCESSION   DAGCYL010000157 DAGCYL010000000
VERSION     DAGCYL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.159"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            725..811
                     /locus_tag="MB216_002311"
     CDS             725..811
                     /locus_tag="MB216_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBT4527102.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="MB216_002312"
     CDS             complement(932..1621)
                     /locus_tag="MB216_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527103.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="MB216_002313"
     CDS             complement(1866..2780)
                     /locus_tag="MB216_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBT4527104.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="MB216_002314"
     CDS             complement(2762..3142)
                     /locus_tag="MB216_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBT4527105.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="MB216_002315"
     CDS             complement(3592..3879)
                     /locus_tag="MB216_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBT4527106.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="MB216_002316"
     CDS             complement(3899..4123)
                     /locus_tag="MB216_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBT4527107.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="MB216_002317"
     CDS             complement(4578..4748)
                     /locus_tag="MB216_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527108.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="MB216_002318"
     CDS             complement(4763..5296)
                     /locus_tag="MB216_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBT4527109.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="MB216_002319"
     CDS             complement(5317..6159)
                     /locus_tag="MB216_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4527110.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAGCYL010000158         6068 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.160, whole genome shotgun sequence.
ACCESSION   DAGCYL010000158 DAGCYL010000000
VERSION     DAGCYL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.160"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            832..1566
                     /locus_tag="MB216_002320"
     CDS             832..1566
                     /locus_tag="MB216_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4527111.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="MB216_002321"
     CDS             1582..2751
                     /locus_tag="MB216_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4527112.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="MB216_002322"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="MB216_002322"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBT4527113.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="MB216_002323"
     CDS             3841..4767
                     /locus_tag="MB216_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBT4527114.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="MB216_002324"
     CDS             4786..6036
                     /locus_tag="MB216_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4527115.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1246 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt aaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DAGCYL010000159         6062 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.161, whole genome shotgun sequence.
ACCESSION   DAGCYL010000159 DAGCYL010000000
VERSION     DAGCYL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.161"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..476)
                     /locus_tag="MB216_002325"
     CDS             complement(<1..476)
                     /locus_tag="MB216_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBT4527116.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIALSAFLSALLAFCFKKSLETWFHTVDRRMIGTIGTLILFTYY"
     gene            complement(617..1219)
                     /locus_tag="MB216_002326"
     CDS             complement(617..1219)
                     /locus_tag="MB216_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBT4527117.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            1465..1671
                     /locus_tag="MB216_002327"
     CDS             1465..1671
                     /locus_tag="MB216_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527118.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            1984..2703
                     /locus_tag="MB216_002328"
     CDS             1984..2703
                     /locus_tag="MB216_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBT4527119.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(2784..3467)
                     /locus_tag="MB216_002329"
     CDS             complement(2784..3467)
                     /locus_tag="MB216_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBT4527120.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            3699..5885
                     /locus_tag="MB216_002330"
     CDS             3699..5885
                     /locus_tag="MB216_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBT4527121.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2274 a         1018 c         1149 g         1621 t
ORIGIN      
        1 atatagtatg taaataatat caatgtcccg atcgtaccga tcatacggcg gtccacagtg
       61 tgaaaccaag tctctaacga ttttttaaaa caaaaagcta ataaagcaga taaaaaagcg
      121 gataaggcaa tgtgaaaata ctgcaatgaa tctcctggtt caataaccga agatttcaaa
      181 acaaaaaaat cgccgatgct tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa
      241 ggaagcgcgt aaaccaattg ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc
      301 attcctatag caattagagc agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa
      361 atacaagaaa tcagaatgac tcccgcaaac agatatttgt atcggatgtg atggtcaatc
      421 aataagatac aaattgtgta aatcaaatat tgcaacgcta tggaccataa aaacataaag
      481 tacagctccc attgattttt accaaattat agcacaatta gcgtattatt tttttaattt
      541 ttcagggatt ttgaattcag aactcatacc aaagcagcag ccagatacaa aaaagtcaga
      601 taagttagtt aattgtttaa tgattgtttt tttcatgaaa gattcctcca aaattcatca
      661 agtagtttgt aataggaagc aacgtagcta tttctaaaac aatcccaagt agcaaatagg
      721 tagaaaagta agaagctgag aaaaataaag taatgagaga aaaaattaga aacatgctga
      781 tcgccttctt taataatggg ggacgtttct cgggttcaat gggattgatt gctgtacctc
      841 ttgatccata gcgaagcagt aacaaaaagc agctgattaa caaaaagacc atcattggga
      901 aggagatagt caattgcaga tgtttcatca ccaagggaac gccccataaa taaagtagtg
      961 tccacagcag acaaacgaat tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc
     1021 atatataaga aagaaaggtg attcctgttt ccgcccatga gcctgtcacc agagaaaaga
     1081 ctagaacgac cgctagtttt ccaagattat tcaataaaac ttctaaggca aattttactt
     1141 ggacatacac catatcttct tccgatttgt ctccgctgaa taaggcttct aataatttgt
     1201 tcacaacttt atctttcatc tcatacgtac ctctaccaca aattttatgc taaaaaaaat
     1261 aaaaaacgtc ttttgtttca taaaaagtgg ttttgacatc aaaaccgtca tgatttccta
     1321 aaaaaatctt cgttttccaa gaaccttacc aaaaaaagtg acagttttga tacgaaaagt
     1381 actttttatg acaagtttgt gctttttcga gtgatttatg ttaaaaaaat tactgacctt
     1441 ataaaaggtg atggagggat tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca
     1501 tatgcattta aatctgattt atttgaatca ccaataaaag gaaaaataga aaaaatctat
     1561 gaaaatagtg tgatgatacg tgtcatgaaa tgcgcagtag aagacaatga taccgctcat
     1621 aatctgcact ggcagttagt tgtacgcaaa tcaagtacaa tagaagaata aaaacagata
     1681 aaatgttgag agcataaaga ggttgtgagt ctccgtttag ctctgagtta caaggaataa
     1741 tgttaaaaaa cagcttttca tgttttatga atattgccac ttgtgaccga ggtgttggca
     1801 ggagaatact gtttatttga aaaaagggct gcgacaagac ttttgtcaca gcctttattt
     1861 tcatgaatag gagttacttt tggctacttt tagagaagta gcttttaagg agcagataaa
     1921 gtcaaggctt ttatttctat aaatgaagta gtaaaatgaa gaacagattt tattacagga
     1981 aggatgccga taattgtgat cattgttgca ggaacgatcg gtgcagggaa aagcacactg
     2041 actgaaatgc ttgcccaaga tttagaaaca aaaccttttt atgagaacgt ggaagataat
     2101 gaagtattgc cgctatttta ttctaatcca gaaaagtata cttttttatt acagattttc
     2161 tttttgaaca aacgcttttt ggcaatcaaa gatgctttca gccatgatga caatgtacta
     2221 gaccgctcca tctacgaaga tagtatgctg tttcatttga atgcagattt agggcgtgtt
     2281 tctgaagtag aagtaaagca gtatgaaggt cttttagaaa cgatgctgaa ggagttagaa
     2341 gaaatttcgc cacaaaagaa accagatcta ctggtttata ttcgtgtatc ttttgaaact
     2401 atgttagcga gaatcaaaaa aagaggccgg gaatatgaac agctagaaca agatcctgaa
     2461 ctctattcct actataaaga actcaatcga cgatacgaag agtggtatga acagtttgat
     2521 atatgtccca aaatagtgat cgatggggac aaatatgatt tcgtagccga tccggcatgt
     2581 ggagaacaaa tcgtacaaga aatcaagatg cgggcaaaga aaatgacaga agaggcagat
     2641 gcccagtcga tccgctacac tacaaataac ccgaaagcaa agggaatctt tacaactaaa
     2701 taatataaaa actaagcaaa ttgctcactc gcagatattt ctctgtgagc agcatcttgc
     2761 ttagtttttt tgattcattg agatcaaata gtgaaccgat tcgtatgata aaattgccat
     2821 aaatgaaatg tctgagtgag ggccatgatc aaaaataaaa tggaaacgat caaaatcgaa
     2881 tgggcgccaa acagatgatg acagaaaaca aggacaatag ctcctgcgat aaaggcaaga
     2941 acgacactta gatagtggaa aaatcgatca aaatctgctt tttctttaga aatacaataa
     3001 tcatataagt tcgttgctac cccttttaaa tttccagttg taaataaatt agtgtaggca
     3061 cgcccgttga ttttatcaaa agctacccac tggatcgatg cagtaaaagc aatcaaaatc
     3121 gttactaatg cgggatgctc atggacgggt gtcaaactaa caaagaaaaa aatcactgct
     3181 tcataatata gaacaaacaa gcgccagaaa tgtttttctt tttttctaaa ataatcaatt
     3241 agatactttg ttagaagaat ccctaagaaa aagaaaaggg tagacaatac ttttttacca
     3301 acacttagcc attctccgtc aaatgcttgg atgatcgata gaatgatatt gccagtttga
     3361 gcagaggcaa aagcaccata ttggatataa gtataactgt ccatggctcc tccgataaat
     3421 gtcagcaaaa gtcctattgt acgcccctcg tgaaaggaag tctgcatttt tttcatcacc
     3481 ttttatgaat atttttgtct tccttatctt agcataaacg attgaaaaaa aggaaaaatt
     3541 ttcagaaaac taaaatcata tcgcttccca ttttcatttt tagaaaggtc tgttagtata
     3601 aagaaaaaat agacaacgaa actttaaata accaacttga aataaccaac ttgaaataaa
     3661 caaattcaaa gaagaaagat gggaaggaaa tcaatcagtt gaatgaaaaa gaaattctcc
     3721 gattacaaaa agaattgaaa cgctgggaga acaagtataa aaagcttcaa caagtctttc
     3781 ggctacaaga agaaatcatt gctacctatg aagcggctta tggcgaagca cccggactta
     3841 ttgaggaaga agtacaagta aaagaaagtg aaggaaacag ctctttcttt aaaatgatgg
     3901 agaaacgttc ctataatcgc ttcaatcaag aacaaaaagc agctatcaca tatgatatga
     3961 agaaacatct tcgaatcata gcaggagctg ggagcggcaa aacgcaaaca atctgtgcaa
     4021 aagctgttta tctgatgacg caaaaacaag tagacgaaga acgcatacta atgatcactt
     4081 ttacaaggaa cgctgcaaat gaactaaaaa aacgagtaga taatttcagc cagagaaaaa
     4141 ccgctgtcca tatcggaaca ttccatagta tcttttttag gatatacaat gaaatttgtc
     4201 gaaaatttcc tgaagtagca atgcaaggaa tacaaggaga cttttcaaag gatactgcac
     4261 aaaaagtgaa tgctgttttg caacagctga tacgtaaata taatctttat atatttgatc
     4321 aatatggaga aaaaacgatt gcttctcggt tagactattg gcagaatatg aacctttcag
     4381 tagaagaaat ggttcagtta gttaaagaaa aatatgattc cattgataag aatgcacgac
     4441 agccgatatc agaacgactg tatggattgc tcacggaact ccaagaacaa aaaagaagcc
     4501 agcaattgct agagttcaat gatgtactcc aaaatctgaa aagcgctttg gagaatgagg
     4561 agatccagca ttatatcggc caaaaatatg attatctatt cattgatgaa tttcaagata
     4621 ctaatccact tcaatgggaa atcgtgaaac ggatgacaaa ggaaaacagg atcaagttga
     4681 ttgttgttgg agatgatgat cagagtatct atggattcag aggatcagag ccagcataca
     4741 tcaagaactt tgaaaaagaa tatcctacca aaactctttt cttattaact aattatcgtt
     4801 ctcgggcagc tattgtccaa ggagcaaata gattgatcag ctataataaa aatgatcgga
     4861 taccaaaggc gatgattcca gcacaaaaag aagcaggtgt tatggaagcc tatttgtttt
     4921 ctgatactaa agcagaaagt cagtggctaa tcaaacaaat tcagacattt attcaaaaaa
     4981 atgggaaata taaggaatca atcatattat accgatcagc ttcacaaacg cagcagcttg
     5041 tccaatctct gctgaaaacg aatattcctt ttgtactgga agcagatagt ccatatgaag
     5101 gtatctttgg tatcaagtct ttccaacatt tctatcaaaa aattgttcat tggcaaacag
     5161 cactgaatct gcaaaagaaa aatcaggcct accagcaatt gctccggcag ctgatggcag
     5221 actgctacct gaaaaaaagt gaatgcgatc aatacttttc tcctaaatat caaaatatag
     5281 ccgttgcaga ttatattttg tcgataagcc ccaatttaaa atcaaaatct gcagaaatcc
     5341 gagagtttga ggaagcgttg cgtaaaacag accagcatca agaaatctat tcgcttgttc
     5401 aaagctatct tcatctgcca cgtatagcta aggaaatcga ccagagtgat caagaatgga
     5461 tacgagaaga agtaaagcag catgctactt tcagaagact ccaagcctta tcgcaagaaa
     5521 cccaagcgac agctaaagaa ttaaaaaccc gcttgattgc ttacagacaa ggaaaacttg
     5581 atgcactatg tatccagagc attcacaaaa gtaaaggtct tagttaccga aatgtctttt
     5641 tgattggttg taacgaagga agtttgcctt ataacggagc gacagaaaag aaagtaatcg
     5701 actttaatga aataaaagca gaaccagtga cgacgataga agaagaacga agactatttt
     5761 atgttgcgat gactagagca agaaatcgtc tgtatctatg tgtacctcaa atgaaaaatt
     5821 caagaaaatt gaaagtatcg agatttatca aagaaaccgg gagtcgtgtg aaaaaaggaa
     5881 agtgatagaa gagagggtgt gacagaagtg gtcagtttca agaaagaaga aggaatgtac
     5941 gaaaattgct tctcaaattt ttgtgaaatt tcagcttatt ttcgaagaag ctatttctgg
     6001 aacaccgttt attcggaaaa aggaggtgtg acaagaattt tgtcacacct cccttattta
     6061 tt
//
LOCUS       DAGCYL010000160         6017 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.162, whole genome shotgun sequence.
ACCESSION   DAGCYL010000160 DAGCYL010000000
VERSION     DAGCYL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.162"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(58..360)
                     /locus_tag="MB216_002331"
     CDS             complement(58..360)
                     /locus_tag="MB216_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527122.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(580..946)
                     /gene="ssrA"
                     /locus_tag="MB216_002332"
     tmRNA           complement(580..946)
                     /gene="ssrA"
                     /locus_tag="MB216_002332"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1117..1890)
                     /locus_tag="MB216_002333"
     CDS             complement(1117..1890)
                     /locus_tag="MB216_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4527123.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2034..2384
                     /locus_tag="MB216_002334"
     CDS             2034..2384
                     /locus_tag="MB216_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBT4527124.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2365..3081
                     /locus_tag="MB216_002335"
     CDS             2365..3081
                     /locus_tag="MB216_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4527125.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3081..4529
                     /locus_tag="MB216_002336"
     CDS             3081..4529
                     /locus_tag="MB216_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4527126.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4599..5108)
                     /locus_tag="MB216_002337"
     CDS             complement(4599..5108)
                     /locus_tag="MB216_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527127.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5098..5823)
                     /locus_tag="MB216_002338"
     CDS             complement(5098..5823)
                     /locus_tag="MB216_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527128.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2046 a         1048 c         1170 g         1753 t
ORIGIN      
        1 cttttataag tatggtgata aaatccgttc cgggctacct tttttaggta gcctacttta
       61 atctttatac ctttctggat caacgaaagt atacttcaaa tagtcataac gccgatgatc
      121 gctccgtgcg tctggcacat cagtcacgat atcaaacaaa aaatacacat ctttcttcat
      181 tctggttttt gttgccggaa ttttaaagta gttcttatta gagtaataga gattgattaa
      241 taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt
      301 gacaaatcta ttcgaaggat caaatttaaa ccataattgt gtttttcctt ccatcaacat
      361 aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta ggaatatcat
      421 tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct attttgaatg
      481 ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac ccttataaaa
      541 taaggattta agcatctagc gaaatctgct gaaatctcac aatggagacg gcgggagtcg
      601 aacccgcgtc caaacacatc gccacttaaa aatctacgtt catagtcaac tcatttaagg
      661 attcacttta cagcttgccg agtgacaggc attctgtatc gctagtctga tgttctcttt
      721 ttcaacttac agacggaaag ttgaaacgta tcccactaaa gttaggaccc ttactcgagc
      781 acatgggcga tgccgagagg atctacgcta actgttttta ggcagctaaa gcgtaagagt
      841 tgttttcgtt tttagcagtt atatttaact gtaacgtttt tacgtagacg taacctacga
      901 aacgcaattc aagctcgact gtgcctgtcg aatccgtaac gtccccgtta gctaacaagg
      961 gttacgtgta tttttcatac aaaattagta tagcatatat tgggataaat tcaataaaaa
     1021 agcgaatcat atcccattat ttatataata actgcccctt tcttaagaaa aaataagcga
     1081 ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa aatacttcaa
     1141 tgcgtggaca attccgcctt ctgtattttt ctttgtgata aaggtagaaa gttcttttaa
     1201 tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca aggcaaaatc
     1261 attcggacca tcgccaaatc caaaagtagg aactttttcc agctgaagtt ctttttgcag
     1321 acgtttgaca cctgcccctt ttgagattcc tttcttcact gtatcgatcg agtaaggacc
     1381 attccgataa aaagttagct caggaaaatg ttcgtgatag tattcgtcac ctgattgaga
     1441 taagatcagc aacatattta ctgtgttttc tttaaagcct aaaggatcaa tttccggaac
     1501 atctgaatga atgaagtcgt aagcattgat cattgtttgg ctatgaccgg tacaccagat
     1561 tttctgatcg ttatagtaag aaagttcatg accgttcaac tgtacatgat catacatttt
     1621 ttgacattct tctgaagaaa atgtttgaga atagatttct tcgccgtcta ctcgaatgaa
     1681 agaaccattc atggtgaccg cagaagtaat gcccgcatct tccatgatag agtggatttc
     1741 tgcttctgta cgtcctgtag cgatgacggg gagaacattg ttttctttta atgcgttcat
     1801 agcttttgcc acttcgggtg tgatttttga tttcgcatct agtaatgttc catcaagatc
     1861 aaagaaagtg attgcacgat aatttgacac aaataaaact ccttttttat tggaataagt
     1921 ttatcttaac aaagaatgaa caaaagacaa acctttcgtg aagaattcct gaacatattg
     1981 gttcagtcaa caatttagag tatactataa gaagagaatt taggagggaa agaatgattt
     2041 ttttcagtat tggtatctta ttcttaataa ttggcagtct ttttcggatt ctgccctcaa
     2101 aaggaaatct gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca gacgctcatt
     2161 ggcggctggc tcaaaagaca agcggaaact ggttttttct tatgggctta ctgatggcat
     2221 tgatcggcta ttatttaaag acatctgggc atacgaatta ttttctgatt gaaatgcttc
     2281 tgctagtatt tccaattatg cctattttta ttatgacaga aaagaaatta cagaaatatg
     2341 acctggaaac tggagggaac gataatgaat attttaatga ttgaagataa tcaatctgtt
     2401 tctgaaatga tgcagatgtt tttcttaaat gaaggttggg aagcaacttt caagtatgat
     2461 ggaaaagaag ggctcgatgc ctttttagaa aacccacaac attgggatat gattacacta
     2521 gacctgaatt tgcccacact ggacggtatg ctagtaggaa gagagatccg caaagtttca
     2581 agtactgtgc cgattatcat gctgacagct agagattctg aaagcgatca ggtgatcggc
     2641 ttagagatgg gggcagatga ttatgtcaca aaacctttca gtcctctgac gcttattgct
     2701 cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag caagagaaga agcagtagtt
     2761 tcagatgatc attttgatat cgagactagt catttcaaga tgaatacgaa aacacgagaa
     2821 gcgtatctgg acaaccggcc aattgaagga ctgacgccta aagagttcga tctattgttt
     2881 actttagcaa aaaaaccacg tcaagtattc tctcgggagc agttgcttga attagtctgg
     2941 gattatcagt actttggtga tgaacggaca gtggacgccc atatcaagaa attacgacaa
     3001 aaaatcgaaa aggtcggacc acaagtcatc caaactgttt ggggagtcgg ctacaaattc
     3061 gatgattctg gtgttgccta atgcgttatt tgtatcagca actcttagct ttctgcggaa
     3121 tggtcgcgtt gatcttattg atcgttggag tttcttttac acaactgaca aaacaaacga
     3181 ttgaagaaaa caattaccag caactgtttg gatatgctga atcagttgaa aagacaacgc
     3241 aaacctatgc cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa aatgcattgc
     3301 tgctgacaga gcaagtactg acagaacaaa atgtgaactt tatatttatc gataaaaacg
     3361 aacgtgtcat ttatccgact aatacagctt tactgaattt taccttaaca gctgatcaat
     3421 ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc gaataaaaat attttaggac
     3481 aagcacaggc gacctcttac gcacttgtgc catttaattt gaatcatgaa ttctatggag
     3541 cacttgtagt tagtcaacct gcgcgaaata tcgataacag cgttcgtcca attacactga
     3601 acctgttcaa aggatttatt ttttccagta tcgttgcagt tattgccagc tatgcttttg
     3661 cagcattcca agttaagcgt atcaatcggt taaggaatgc gacgaaagaa gtgacgaatg
     3721 gtaattttga tgtacagctc cccgtacatg acaaagatga atttgatgag ttagcagatg
     3781 atttcaataa aatgacgaat tcattgaaag aatctcaagc agaaattgaa gaacaagaaa
     3841 atcgtcgaag acaatttatg gcagatgctt ctcacgagat gagaacacct cttacgacaa
     3901 tcaacggact tctggaaggg ttagaataca atgcaatccc tgaaaatcag cgagaaaacg
     3961 cgattaaatt gatgaaaaat gaaacagagc gtctgatccg tttggtgaat gaaaacttgg
     4021 attatgaaaa aatccgtacg aatcaaatca gtatggtcat taaaaaattc aatggaacag
     4081 aaacattgaa aaatattgtt gcacagttag aagctaaagc agaagcagca ggagatacat
     4141 tgacattgaa agccgttgat gatattgatg tttacgctga ttatgaccga tttgtccaaa
     4201 tcatggtcaa tattattcaa aacgcgatac agtttactga aaacggccaa atcacgatta
     4261 ctttagaaaa aggctatctt gaaacgatca tcacgattga agatacggga attggaatgt
     4321 ctgaacaaca aatgaaaagt atctgggatc gttattataa agtagatcct tcaagaaaaa
     4381 atacaaaata tggtgaatca ggattaggtt taccaatcgt gcagcaactt gtacgcttgc
     4441 ataaaggaaa attagaagta gaaagcgaac taggaaaagg aacaaaattc gctgttcgtt
     4501 tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat catgaatcga
     4561 tcttctatac cgcttgtttt tactataaaa tgattgattt agcccgcggg ctctaaacga
     4621 tcaatataag gactttgagc aatagtagca tcatgagtaa agagtaagat accttgattt
     4681 tcttcatgag ctaaataaga aagcaagtgg agtagctctt ggatctccaa tacactaagt
     4741 tcatcaaatg tatcttcaat aacaatattg tttcgcttgg aaagcaaggc atgaagtaat
     4801 tgcagcttta ctttttcaaa agacgtcaac tcagctgccg cacaattaag caaggaagga
     4861 gagatatcaa gtttttcttg ataggccaaa cgatcttgtt tgatttcttt ttccggtaca
     4921 tccaacagga catggtcttt aagattcaga taaggaaaca tcggccaatt tttttggatg
     4981 atcgcaaaat tatccagttt tgcaacaaga acctcttttt tcaagtgctc caatatttta
     5041 tgctgttttt cgagaggaca caaaataacg gtcaagtgtt cagcatgaat caaatattca
     5101 ggtttcatta cgtccaggcc tcctactttg caaatgatgg ataaaaaaag tgaaagggaa
     5161 tagactaaga atgagacagc atccactgag cccaataaga gtttgggcaa atcccttaag
     5221 tatatctgtg aaaaaaagat cattttggaa atcgatggta aaaaaagtct gttgatccat
     5281 cggaattgta agtccgctta ccgaagtcaa gatatcacgg atatctggca gttcagtagt
     5341 ctttaagaca agaaaattga tcctttgaag aagcgcttca taaactgact ggaagcttac
     5401 gatccacaat aaaaccaaga tacaacaaag aaagagagga aagaacagct gccagaaaat
     5461 aaaaaggaat aatttggttt tagaaagacc agtcaggtgc caattgatca attcctgctt
     5521 gttttgcctg actgaatagt aaaagaaaaa gataaaaagg aaggcaaata gaaaaaataa
     5581 actgaatatg ccttttatat aaaatcggct agctaattct aaagccggaa gaagctctgt
     5641 agatgcagaa gcgccaataa atgaaagccg atcttggact tgattggcta aagccgattg
     5701 caaatggtaa agagtcaggc atgctatcaa taaaaaagca gaaaagcttg aaaataaaca
     5761 atagagtatt gtctgctttt tgtaataaat taatcgagca gtagcatagc ggattatttt
     5821 catacttttc acctctataa aaagtataga aaatgaatat gaataaagta tgaaattcct
     5881 ttagtgaaat cacgaattaa aaccagaaaa tattttgtaa aaagcaagag gttgtgaaga
     5941 agctgcccta ttctattacc gtagatgcaa gcttttgaac actgtttatt cgaaaagagg
     6001 agcagcgaca ataattt
//
LOCUS       DAGCYL010000161         6001 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.163, whole genome shotgun sequence.
ACCESSION   DAGCYL010000161 DAGCYL010000000
VERSION     DAGCYL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.163"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            59..1009
                     /gene="yidC"
                     /locus_tag="MB216_002339"
     CDS             59..1009
                     /gene="yidC"
                     /locus_tag="MB216_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBT4527129.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(1188..2162)
                     /gene="lacD"
                     /locus_tag="MB216_002340"
     CDS             complement(1188..2162)
                     /gene="lacD"
                     /locus_tag="MB216_002340"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBT4527130.1"
                     /translation="MKKMSIEKQECLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDESVEINVQKQIVIERIDSECEAEGMPFF
                     LEILSYDATISDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVKGYG
                     QETCYSQAEASTYFYEQSKVTEQPFIFFSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(2159..2617)
                     /locus_tag="MB216_002341"
     CDS             complement(2159..2617)
                     /locus_tag="MB216_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4527131.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKTEIATILTKEIGEDI"
     gene            complement(2614..4077)
                     /locus_tag="MB216_002342"
     CDS             complement(2614..4077)
                     /locus_tag="MB216_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4527132.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEGLEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            complement(4059..5009)
                     /gene="pfkB"
                     /locus_tag="MB216_002343"
     CDS             complement(4059..5009)
                     /gene="pfkB"
                     /locus_tag="MB216_002343"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBT4527133.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMGCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            5152..5901
                     /locus_tag="MB216_002344"
     CDS             5152..5901
                     /locus_tag="MB216_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4527134.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFTDRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMREN"
BASE COUNT         1822 a         1322 c         1030 g         1827 t
ORIGIN      
        1 catgcgcttt tttagtatag ttaagtttgt gtaaaaatta tagaagagga agtattctat
       61 gaataaatgg aaaaactggt tattgggatc cggacttgtc agtatcctgc tctttttatc
      121 tggctgtgtg cggatggatt caaacggaaa ccctgacact tcaggaatcg tttatcgagt
      181 actcgtccat cctatgggcc aagcgattac atatctagtt gagaatttca attggtctta
      241 cggctgggca gttattgcaa tgactgtgat cgttcgtatc atcatcttgc cactaggtat
      301 cagtcaatcg aaaaaaacaa tgatccaatc tgaaaaaatg caagcgttaa aaccacaagt
      361 tgaagcagca cagcaaaagc taaaagctgc aacaacacgt gaagaacaaa tggcagcaca
      421 agcagaaatg cagcaagtat atcgcgaaaa cggattgagt atgaccggcg gaatcggctg
      481 tctgcctcta ttgatccaaa tgccgatctt ctctgccttg tattttacgg ctcgctacac
      541 agaaggtatc cgtgaatcat ctttttacgg catcgatcta ggctcaccaa gtatggtgct
      601 tgtggccatc gcaggggtcg cttatctgct tcaaggatat atttcaacaa tcggtattcc
      661 tgaagaacag aaaaagacga tgcgtacgat gctgatcgtc tctcctgcta tgatcgtctt
      721 catgtcgatc agtgcgccag ccggtgttac actttactgg gtagtcggtg gtgtgttcag
      781 ctgtctgcaa acattcatca caaacgttat gatgaaacca cgtttgaaag cacaagtggc
      841 agaagagttg aaacaaaacc cgccaaaaca agtcgtcact ccaagaaaag acgtgacaga
      901 atcagcaaag ccagctgaca acaaacaagc aacaaaacaa atcaaacaac caaaaaacag
      961 tacaaataac aaaggccgca acgcaggaaa gcagcagaag cggaagtaga tagtaaataa
     1021 gagtgtaata aaaattcatt aacagtttta gtaattaaaa atatcgacca agatttttga
     1081 aaaagaaatc tggtcgatat ttttatttaa gtgaatgttt aatttgaatc atcgattgat
     1141 cgctatctaa ttgcagcagc atctttttat gaagaaacaa ctttttctta tctatcaacg
     1201 tagacaggtg ttgctgtttc ttctaatagt gtacttaatt ctttaatatt ttgttttcct
     1261 tcttctaaca tccaactact cgctttttct tcgccttcgg taacaaaagg tgctactccc
     1321 ttcgcccatg ttgctcgtcc acataagaca ccattgaatg tcgaacctga ttgttttgca
     1381 aaacgcaatg tatcttgaaa tagtgcagcg ctgactcccg cactaaaaaa aataaacggt
     1441 tgttcagtta ctttcgattg ttcatagaaa taagtggatg cctctgcttg actataacaa
     1501 gtctcttgcc catatccttt cacaaatctc atattcactg gtacttctac tttcaataca
     1561 tccacttgat aacgttcttt tgaaaattct cgcatggctt caatcacttt atgcggtttt
     1621 acttttgcat aggcaagaga agacggatcg ctaatagttg catcataaga aaggatctct
     1681 aaaaagaaag gcataccttc tgcttcgcat tctgaatcaa tccgttctat aactatttgt
     1741 ttttgcacat taatctctac tgattcatca acgtcatagt acaataaaaa tttacaagca
     1801 tccgcaccca cttcttttaa ccttttcact gaccaactat tcagtaaatc tggtagtcgg
     1861 cccggcattt tcgcatcata gccggttttt tcataagcta gcaacaagcc acattctttc
     1921 tctcgaagcg ctgctgcagg cagaccatat tctggatcga gtaaaatagc ggaagcatac
     1981 ggagtcaggt actgagaaac catctgttta aattggatga tatcttctga tgtagcttct
     2041 ttatattggt tgatcatttt tttcaaagca ccacgctggt cgattgctaa cgcgctaata
     2101 attccagctt catccgtcaa tcgcttcaaa cattcctgtt tttctatact cattttcttc
     2161 atatatcttc acctatttct ttagttaata ttgtggctat ttctgttttt gattttgctg
     2221 ctctcaactg ttctgtaacc tcttttcgca tcaatgatcg tgcaacgaga gacaataatt
     2281 tcaaatgagt cgttcctgct tctgaatctg ggataaataa cgcaaaaata aaatcaactg
     2341 actgtctatc taaactgttc cactcaatcc ctttgttcag tgtgacgata atcaaactag
     2401 gttcgttgat tgtttgagac ttcgcatgag ggatagcaaa acctgacatt attcctgtag
     2461 taccttcttt ttctctttct agaagttttc gatatacttc tttttcgtca tgagcgattc
     2521 ctaaacagaa tgtttgcttt gctaaaaact taaatgcttc ttcttgacta tctaatggct
     2581 gttgtaaaaa aatgttctct tcatgaatga ttgtcatgat aagcctcctg ctcttccttc
     2641 ttcgataact gtacgatttg ctgctttatt atccatcttc ttgtctttat cagttactct
     2701 tttcttttgg atcaaaccta gtatcactcc accaacgatc gaaccaataa gaatcgctgt
     2761 aacccattgg atttttcccg tcactacagg taacactaaa aaaccgccat gtggggctgg
     2821 tacttggaca ccaaacagat aggtcaagac agcagcaatt gatgagccta acatcatcgt
     2881 tggtaatact cggatcgggt ctttcgcagc aaacggaata gctccctccg taatatgagt
     2941 tgatcctaaa ataaaattaa ccaaacctgc attacgatca ttagtatcaa aatatttacc
     3001 aaacaataaa gttgcaaaac cagtaatcaa cggtggtgca atgcaagcta ctgacacgcc
     3061 agccataaaa gtcgtgtttc cttgtgccaa aagagctgta cctgttacat atgccgcctt
     3121 attaatcggt cctcccatat caaacgcaca catgcaacca acaatcacgc caagaacaag
     3181 tggattcgaa tgttcaaatc cagctaaaaa gctcatcatt ccttcattta tagccttcat
     3241 tggacccgcc agcaaagaca tcacgatgcc gataaacact acaccaataa ctggatataa
     3301 gaagattgcc ttcaagccat ctagttgttt aggtaaaatt gtcagtagct tttgcaaaaa
     3361 taagataaaa taacctgcta agaacccagc taaaatacct cctaaaaatc cagtaccgcc
     3421 actcatggcc acgattccac caacaaatcc aacgactaac cctggacgtt tcgcgatccc
     3481 ttctgcaata taagctgcta aaactggaac catcatcccc attgctgcat cgccaaccgt
     3541 ttttaacatc gccgctgtag cattgtattg tgcatggtct ggatcaaaag aatagattcc
     3601 ccaaacggca aatgaaatag caatcaatac tccgccagct accacgaaag gcaacatatg
     3661 agagacgcca ttcattaaat ttttatagat gctgcgacca aaattagcac cacttatcgt
     3721 ttctgattct aaagtatcaa cagcttcatt atctgctaag atggtccctt ttcctgccaa
     3781 tgcttcgtca atcagccgtt gtgcatcctt gattcctgta ctgacagaaa catcaatgac
     3841 tcgttttccc gcaaatcttt ctgaatggac atctttgtct gcggcaataa tgacagcatc
     3901 tgcctgtttg atttcttcag ccgttaattc attctcgatc ccaatttgtc catgggtctc
     3961 cactttgatc gtgatgcctt tttttctagc tgcttgctct aatccctctt gcgccatgta
     4021 ggtgtgtgca attcctgtcg gacaaccggt tgctgcaatt agttgatatt ttcccatctt
     4081 tcgaacctct cttccttgat tttgatttgt ttacttaatt cctccacatc caaaaaatca
     4141 gtcagtcctt tacgaaaagc agtagagctg cctgctgcta tgcttttacg taacgtttca
     4201 tcaagcggtc gtcgattcat gtatcctact aaaaaagtgc ctaacatggc atcacctgca
     4261 catgctgtat tgacaacttt tccttttggc gagtttcctc gaatcaccct gtctttcgta
     4321 aaaagaatcg ctcctttctc accaagggat aacaaaacat tttctgcacc tgactctacc
     4381 agtcgttgtc cataaacaaa gtaatcttca tctgtaacca tttttcgtcc gaaccaagaa
     4441 gaaagttctt cctcattcgg tttcagcaaa aatggtcgat agggcaaaca acccatgacc
     4501 tcttgatcac tgctatcaat aatcaaatga atctcctttt cctgacaaat ccgactgatt
     4561 tccactaaaa ccttcggaga cattcctctt ggtaaactcc cagaaacaca aagataatcc
     4621 tctgcttgta atgctcttat ttgatttaaa aaattatgga cagctgcctg cggtatttca
     4681 ggtccttgat tcactaattt gtattcttct ctcgtttgat tgacttgtgt aaatacattg
     4741 atacgcgtca taccaggcac ttcaataaac tctgtttgga tttgtttctt ctgtaaatag
     4801 tcttctatat attttcctgt gaaaccagca gaaaaaccta atgccgtact tgggaaatct
     4861 agcatcttta atactagtga cacatttacc ccttttccgt ttgcctgaat gtcatcctcc
     4921 tttgtacggt ttaccataaa aggatgcatc tcctctgttt caataaatag atcaattgcc
     4981 aaattcatcg tgcatgtgta gatagacata accaatgacc tccttgaatc tattatgaaa
     5041 tacgcgaaag agaaaatgtt ttcttaatcg ttccaacaca aagaaaacct ttacatttat
     5101 aaacacatcg tgctattttt taactaactt ttagtgaaag aaggaaagaa tatggatcac
     5161 aaattaagtg aaacagaaca gtacctctgg aactttatcg aacaccacat cctagagatc
     5221 cctaactact ctatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg
     5281 cggacaatga agaaaaaagg ctatgaagga tttacttcgt ttaagcacca tctaaaagaa
     5341 ggagaaaaca agaacatcaa tttttctttc ttagataaag tagacaaagg tatccgtcga
     5401 gccattctaa aaaatgaaca agaagtggta cgtacgatca atatgttgga aatcgggaac
     5461 attgaagatg caatacaaag aatcaaattt actgaccggg tttttgtctt tgcccgagga
     5521 ttttcagaaa tgattggtca agagatgctc gtaaaacttc aattaacagg aaaaaattgt
     5581 caaatgcata ctgatccaga gattatcaaa agcatcagtc ggaaactaac taagaaagac
     5641 agcgttattt ttgtttcttt gaacggcgag acaaaagaac tcgtcaccgc cgcaaagaac
     5701 tgctatgatg cagagattgg ttcgattcta gttacagcca atcattcctc tacactcaaa
     5761 gattattgcg agataaatct cgtaggtttc aaatcagaag gttcttattt ccctgattat
     5821 gaagtacgtt cacgcttgcc tctccagatc atcgcgagaa tcctgcttga cgcctatgta
     5881 ttgcgtatga gggagaattg agaagcagtt tattatggtt ggttacagtt taaaacatca
     5941 cattttttat tctttatatt gtatggactc acgtaaactt tttatgtata aagttattca
     6001 c
//
LOCUS       DAGCYL010000162         5984 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.164, whole genome shotgun sequence.
ACCESSION   DAGCYL010000162 DAGCYL010000000
VERSION     DAGCYL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.164"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..450)
                     /locus_tag="MB216_002345"
     CDS             complement(<1..450)
                     /locus_tag="MB216_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4527135.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="MB216_002346"
     CDS             674..1465
                     /locus_tag="MB216_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4527136.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="MB216_002347"
     CDS             1431..2384
                     /locus_tag="MB216_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBT4527137.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="MB216_002348"
     CDS             complement(2782..3756)
                     /locus_tag="MB216_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4527138.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YNAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="MB216_002349"
     CDS             3871..4305
                     /locus_tag="MB216_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4527139.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5452)
                     /locus_tag="MB216_002350"
     CDS             complement(4430..5452)
                     /locus_tag="MB216_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBT4527140.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5844..>5984
                     /locus_tag="MB216_002351"
     CDS             5844..>5984
                     /locus_tag="MB216_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HBT4527141.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRA"
BASE COUNT         1880 a         1129 c         1031 g         1944 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatta tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tgaatccaag agct
//
LOCUS       DAGCYL010000163         5763 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.165, whole genome shotgun sequence.
ACCESSION   DAGCYL010000163 DAGCYL010000000
VERSION     DAGCYL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.165"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(246..953)
                     /locus_tag="MB216_002352"
     CDS             complement(246..953)
                     /locus_tag="MB216_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527142.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1018..1854)
                     /locus_tag="MB216_002353"
     CDS             complement(1018..1854)
                     /locus_tag="MB216_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4527143.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1872..3281)
                     /locus_tag="MB216_002354"
     CDS             complement(1872..3281)
                     /locus_tag="MB216_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4527144.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3295..5118)
                     /locus_tag="MB216_002355"
     CDS             complement(3295..5118)
                     /locus_tag="MB216_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4527145.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1765 a         1255 c          945 g         1798 t
ORIGIN      
        1 caacgtttaa tgaaactatc tccgtttcat ttcagataca aataaatata gtcgggctcc
       61 aaatccgaaa aaaaataccc ccagtagctt atcctaagct actggagatg tataagtacg
      121 gtcatataac aacggggtag ggtaaataaa tgaaaaagat ggaagaatca ttattcaata
      181 acttttttaa atacttcgtt gtaatcagtt acctgtccgt tcttaaatac cacagaataa
      241 tctttttacc cattttcaag taaccgaagt ggaatgtctt taccaacttg ctctgtcact
      301 gcattactga tacctgttag attagctaat aaatctgata aagattgagc atctgtagaa
      361 atctctgtat cagtatttat cgtgattcct ttatcttcat ttacaagctt ccaagtaggt
      421 ggtatagctg tgtatgtttc gatgaatgaa tcaattcttt catctatcgt cttcccagta
      481 tctacttctg tctcattact cttagatgaa tcgattcttt catccatcgt ctgctccgta
      541 actgcttctg tctcattatt ctcaccggaa actctcttca cacccaaact tatatattgt
      601 ccatctttag tgtgcatagt taaaattccc acgcttttac taataaattc actataacca
      661 gttctttcaa tagagacttc aatgtatttc acactatcat actcaaaaga aaaatagaca
      721 tgtaaaagca ttttgttgtt tacatctaca taataatcct ttgactctac ttgagacctt
      781 cctatacgga atacagcttt actgtctgat atctctatac cagcatcctt attattaatc
      841 tgccaatctc caagtagctc ttctggcaag ctagtagttt cttgtgaaca ctttaccact
      901 tcactggtag aggtcgaaac attcacctcc gcactgttag aagaattttc caaggaatca
      961 ggagccttag cacaaccaac aagtctttat atattgtgca gccgatattt tcttcattca
     1021 gtacaccctt gaaatatgca gtgttagata aagcagttcg tcctgatttg ctgaggcacc
     1081 catctcttct tgaacgtact ccttgatttt caaggccgcc cgatactctt tcggaaaacg
     1141 ctgcttcact gaagccagca aaaaatcatc cttctccggc aactcttcct tcttaatcat
     1201 tcgggcaata aaaaactgta aatgcgtcac aaatcttaag taaagcatcg aatctttatc
     1261 aacctttttt tcgggaaaca gatatcctag catagccaag atatccttta gtgctcttac
     1321 cgagagcatg gtttcttcca tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc
     1381 aatgtttcca gcctcctctt ctggcaactg aacattcatt tttttgttga tcaattggat
     1441 agcatagcag ccgatctcaa attcttttgg gtaatacttt tgaatttcaa tcagcaggcg
     1501 attttgaaaa acaatgcctt tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa
     1561 ggtaaaaaat agacttttat tcagcttaaa gtcaaacttg gcgttggcat aatcgataat
     1621 tcgcttgacg caaataatta gatcatccgg agcagcctca aacagtttaa tgtaatcctt
     1681 ttttagagca tcagaaagtg aatctaattt aaatactttt tggattttgt cagccggaac
     1741 caaatcgcct ttctttagtt gaaatccaat ccctttcccc atcaaaatta cttctttgcc
     1801 ttgcttatct aagcaaagaa ttaaactgtt gtttaacgtt ttaattactc tcattagatt
     1861 caccgttctc tctaagctaa gccatttctt gcgattactt tttgatacca aaagaagctt
     1921 tcttttttta ttcgttttaa ttctttaaga ttctcatcgg ttcggtcaac atatataaaa
     1981 ccataacgct tattgaagcc ttgatgggta ctaacaacat caatcgctga ccaaggtaaa
     2041 taaccaaaaa catctacacc atctgttaaa gctgcttgaa tagccagaat atgtttggct
     2101 agataatcaa ttcgatcctg gtcattaatt cgtctatctt cctcaactat gtcgggtgcc
     2161 ccatagccat tttcagtaat taaaataggt aaatcatagc gatcgtacaa tttcctcaat
     2221 gttaatcgta gtccagtagg gtcaactacc cagccatatg gcgttttatc tacataagga
     2281 ttttcagctg cccggtagac accttcttct cccaacatga tctgttgatc ccccgctcta
     2341 gcggaaacat ctgatccgtc atttttgctg gcagcgatgg tggcagttga ataataatta
     2401 atcgcaataa aatcgggttt accggaggat aaaattgcct gatcctctgc ctcaatttta
     2461 ggatataatc cacgatcatt caaatagttt tcaaacaaat aattgtattt tccccgaact
     2521 gctacatcta agaaagacca gcctctcaat gtttcccaat tatgggctgc aattgcatct
     2581 tctggactgg aggtagcttg atacatagcc gttaaattga ttgctggacc gatttttcct
     2641 ttaagattca actgatgata caagtgaatt actctagctt gagctaataa aacatggtga
     2701 ttaatttgaa agagttcttt ttttgaaggc agctctccac ttggtggcaa accaatcgct
     2761 ccaggatgaa ggatcatcgt gttttgctca ttaattgtca gccaatagtt caccttgtca
     2821 ccatactcag taagcaatac ctgtacatag tccacaaaat aatcgatagt tgctcgattt
     2881 aaccaaccgc cctgctcctc tagaaaataa ggtaaatcga aatggtacat ggtgacaatc
     2941 ggttcgattc cctgattcag gcattcatca attacttgat gataaaaaga aattcccttt
     3001 tgattggttt tattattttc aatatccata attcgcgtcc aagcgatgga aaagcgaaaa
     3061 gcttttaagc ctaattcttt aaacagctgt atatcttctt tatagtgatg gtaaaaatcc
     3121 gaggcaactt caaaattagc taccccttct gtatgctgat acttgtcaat aatagacggt
     3181 tgtttcccat cctcctgata ggcaccctct gcctggtagg cactaatgga ggcaccccat
     3241 aaaaacttct ctggaaatgc tgaaagctgt ttgtattgca caaaaatcct ctccttaact
     3301 aatttgcatc aatggttcgt gttcctttaa gcttccgctt gcttcactaa ttcctgcgta
     3361 attttcactg ttggtcacta ccacaatcac tgtatcatcg tagccagcgt ttctaatggc
     3421 agcgaaatcg gcgttgatta ataattgtcc tgctgacacc tgatctcctg cttttacaaa
     3481 tacttcaaag ggttcccctt ttaaattcac ggtgtcgatc cccacatgga tcaataattc
     3541 aataccttct ttagaacgta gaccaatcgc atgtttagat tctaaaatag taatgacttc
     3601 tccctcaaca ggtgaataaa tgctgttttc ttctggatga atggctacgc cctttcccaa
     3661 tagtccctta gaaaagacag catcgttcac ttcagaaata ggacacacgg ttccttttac
     3721 cggattttca acaatgactg cttcggctat tgccgtttgc tctgattcat cagaagtatc
     3781 gtcaaatcca attagataag caatgataaa ggttgcaaca aaggcgatta cacacgtaat
     3841 aattgcgtga acaatattca ttggattttc tccaataaag gctggtaagg ctgctaagcc
     3901 aggggaaaca aaggcatatc tgactaaacc gctcaagcca gcatacaaac cagcaattgc
     3961 accaccaatc atggaagcaa ctaatggttt tttcagcttc aaggttactc catagagtga
     4021 aggctcagta attcccatta aagcagtgat cccagtggac acagctagct gcttcaactc
     4081 tttgttttta gttttcgcgg ctacagcaaa ggtagcacct gattgagcaa tattagaagc
     4141 cagcattcct ggcccattga tcatttcaga acccgtactg ctcaattgca tagtagcgat
     4201 tggcgtcatt gcccaagctg tacccgtaac caccaaaaat ggctgcaaac cacccatcaa
     4261 aaatggaatt aaccaagctg ctttgccgtt aatccattca gcccccattg ctataagatc
     4321 gttcagcaac gttccaaacg gtccaatgac aaccaacgct aaaggcccca taattaaaaa
     4381 taccagcatc ggcttagtaa agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg
     4441 ctctacatag gacatgatcc aaacagtaaa gataatcggt aaaactgatc ccgcataatc
     4501 tgcagctaat accggaattc ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga
     4561 aatatttgga tgaagcaaaa tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa
     4621 ctttatggct gccccatagg ctaatagtac tggcataaaa taaaaggctg catcagcaat
     4681 agtattcaaa atgatgtaag tttgactact gtctgatagt acaccagtca aagtcaagac
     4741 ggccaaaatg gcttttacca tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt
     4801 ggtagaaatg acactaatca atcttgaaat aatactttct tgattgtctg aagaggggcc
     4861 ctttttaatt gaagttccca cttccttttg aatttccttg aaggctgttt ggacttcatt
     4921 gccaatcacc acttggtatt gtccaccgct atccaccaca ctaattacac caagtgtttt
     4981 ttctaaggct ttcgtatcgg ctttcctttt atctttgagt tcaaaacgca gtctggtggc
     5041 acaatgggtt aagctgacca cattttcttt tcccccaacc tgttgcaata catttcttcc
     5101 tacttctctg taatccatta ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc
     5161 acgccccaat caacattgaa gtgttagttt gtcttggtct tgcctgcata atcagtaaca
     5221 tgccaataac gttattttgc tgtaatccaa taatagcaca ttatgatagc gcaatcaaca
     5281 gggaatttaa ttttttatct tttcgtttga ccgtataggg taatataaca gttagatcac
     5341 atcaactagg gtatatctgc gtatacttac tgctaaaata agtatatcta atattaacta
     5401 taccagtaaa accattccta cataaccagc gggaaattcc caaaatcgtg aaagcatcaa
     5461 aaatgcatat aacggatcac acaacttcaa caattctgct cttgggaaaa attgatgaat
     5521 ttggtgagta aaggatggaa taaacagcat tccaacaata taggcaacaa tagtgatact
     5581 tgtttttttg gtaaaaatca gtaagcaata gccaatttca atcattccga gatataaagg
     5641 tatcaataga atgatttggg ctagtattct taaaaattaa ctagcaaatg tttaaactga
     5701 tttatttcta atctacatgt agtaagtatt tcaattccac caattatcaa taaaaatacc
     5761 aaa
//
LOCUS       DAGCYL010000164         5732 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.167, whole genome shotgun sequence.
ACCESSION   DAGCYL010000164 DAGCYL010000000
VERSION     DAGCYL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.167"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            149..1045
                     /locus_tag="MB216_002356"
     CDS             149..1045
                     /locus_tag="MB216_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBT4527146.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1195..2136)
                     /locus_tag="MB216_002357"
     CDS             complement(1195..2136)
                     /locus_tag="MB216_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBT4527147.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2262..3383
                     /locus_tag="MB216_002358"
     CDS             2262..3383
                     /locus_tag="MB216_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBT4527148.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYSLGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3641..5143
                     /gene="eat(A)"
                     /locus_tag="MB216_002359"
     CDS             3641..5143
                     /gene="eat(A)"
                     /locus_tag="MB216_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBT4527149.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5210..>5732
                     /locus_tag="MB216_002360"
     CDS             5210..>5732
                     /locus_tag="MB216_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBT4527150.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSY"
BASE COUNT         1881 a         1018 c         1209 g         1624 t
ORIGIN      
        1 gcgaatttta atagaagaat gatttatttc atcatatttt catacaaatg ttaaattttc
       61 atacaaatat aggaaacgtt ttactggctt tttactattt aaacaggcta tcataggaaa
      121 aagtgacaga gaaagtgagg cggaaaagat gaacaaaaat gacgtactgc tagttgcaaa
      181 aaataaatca ctttattttt tgccttccat ccgctcttta gatgtttggt ccggaaaaga
      241 tttactacag tcggaattca ttaaatcagc tagacaacag ttggcaaatt atcagctgat
      301 cgttttttta gattatggat ttgatatgga gatggctcga cgcgtgcgcc catttaccaa
      361 tgcaaaaatt gtgttatttt tttggaatca cttcaaagca gaacacgagg cgatgctgaa
      421 gcaggcacaa gatgaaccgg caatcgatga gatttatcat tttgattttt tggaagcaaa
      481 agaacttggg ctgaaacata atagttcatt ttattcgaaa catatgaatt tttcttctag
      541 cgaaccaaag acggatctat tttttggtgc aacgaataat gggagaaaag aacgagcaga
      601 aagctataag caggaattta taaaaagaga tgttacgacc aactatttca ttcttccaca
      661 tcgagggaat gaacaacctg gatatcttac ttatgaagag tacctgaaaa aaacagggga
      721 atcgcgcggg attctggaac tgttgcggga gggtcagcaa ggagtgactt tgcggacatt
      781 tgaatccatg tattttcaaa agaaactagt gacggataat caagcgattg ctcattatca
      841 tttttatcgt cctgaaaata tttttttgct tcgagaacgt gatttagatg aattaccgga
      901 atttctgcat acaccattta agccaatcct atcagagcta ttggaatttt atgatgcaga
      961 aaattgggca aaacgattta tcactaacga ttccactatt tatgagcaat acgaatatga
     1021 cctctcctct gttgaaaaca aataaaaaac ctacattttg aagagattat gtactgaaac
     1081 aaacaagcac aattcttatg gcatcatgag agttgtgttt tctttttgat cttttattgg
     1141 gcttaaaaaa agagaaccag ctgctgattc agctgattct catcagagag ccttttattc
     1201 tgcgccaagg attccttctt ttgcatggac taattgggtc aaaaaggcac aatatggtgt
     1261 agcaatatca tatttttgtc cttttcgcca aatggcacca ttgatgtagt cgatttcagt
     1321 caaacgatgg tttttgatca ggtcttggta catagatggg taatgcaacc cgatgttgtc
     1381 aggatcataa gtagaagcaa tatgttggta cacttcttct tgatctagta tgattccttc
     1441 ttttgctgcg atagaagcaa attcagatac gattgttcga accatgctct ctgccgcttt
     1501 ttgcgcgcct agttcagcaa tattacaatc caaaatcgtg cataaaccat tcaatgttcc
     1561 atttacacaa gcttttcgcc aaatggaata acgaacgtta tggctataga tcggatttaa
     1621 atttgcttct tgaaagactt ctacaacttt ttttgtaaat gcttcgcctt ctggttccaa
     1681 gttctctagt tcgatttctc catcacctaa caatgtgact tttccgggac cagctagacc
     1741 agctgtccac atcgtgatcc caaataagat atttttcttt ggcacatatt tttccagtac
     1801 atcttcatga cctaatccat ttagtaaaca caaaacataa gtattgtcag taataatgga
     1861 ttggatcgaa caaaacatat catctaattg attggctttt gttaatgcta cgatcaagtc
     1921 cacgtgttcg ttgctttcga tgatttcttc tggtgaatag attggcagtt ttgcgaccac
     1981 ttctttaccg ttaaagtcag caatcaaccc atttgtacga atggcttcaa tgtgagcagg
     2041 ccaacgatcg atcaagagga cctcatttcc gctttgatgt agcatcaatc cgattcggct
     2101 gcccattgct ccggccccag ctattgcgat tttcatgttt tttctctcct ttattcaatg
     2161 agttcccttt tagtatatag aaaaatggaa gaaaatagca ttgttttgtt tgaaaaaaat
     2221 gaaaaaaagt gtcttactta agaagaaagt gagggataca gatggatatt ttggcggcaa
     2281 ttgattcatt taaaggaagt gcttcttcat ttgaactaaa tcaggcagtt ctaaatggct
     2341 gggaagatcg taaaggaaga aaaatcaatg tgccgattgc agatggcgga gaaaatactg
     2401 cagaagcaat ctattacgcg ctcaggggaa catggcgtac cattgatggg atggatctct
     2461 tattccgtca gcgaaaaatc agctatttat tgacaacttg gcaaggaaaa aacaccgcag
     2521 tcatcgaatc tgcacaagca ttggggttag atctacttga taaaccaacg gatcaaacta
     2581 tccgacttgc ttccagttat tccttaggag aacttattaa agatgcactg cttcaagaag
     2641 tagaacaaat tattgtcact ttaggaggaa gcgggtgtag cgatggtggt ctaggattac
     2701 tccaatcctt gggtgcttct ttagaagggt acacagaagg aaatccttta ttatctacta
     2761 aaaaacttca ttttgatcga agaaacgttc ttgtttctaa agtaaaattg attatcgctg
     2821 cagatgttac caatccatat acaggtcaac aaggagctgc ttggcaattt ggaagacaaa
     2881 aaggaggaat agatacgacg ctagctttct tagataaaca agcaggaaag attgctcagc
     2941 aaataaaaga tttctgggag gtggatttag atgatattcc aggaagtgga gcagcaggcg
     3001 gtattggtgg agcccttttt ttgttgggag cgaagatggt ttctggtttc gatttagtca
     3061 gccaaatcac tggattggaa aaaaagatcg aacaagcaga tgtcatcatt acaggagaag
     3121 ggcggatcga tcagcaaacg atccacggaa aagttccata cggtgtagca gtgcttgcaa
     3181 aaaaatatcg aaaaccaata atcgctattt gcggaagtag agaccaagag ctagaaggac
     3241 tttctgattt tctcccaatt gtcttaagta ttcatttggg tccaataggt ttagaagaag
     3301 caatggaaca caggcaaaca ttagaaaaaa caaaaatctt aggacagaca cttagcagat
     3361 ttttcaccat cctcccgaag tagttgacaa aatcattttc tttatgctat cgtaatttca
     3421 aatataggac aaagtgaggg atccgtgcat tgaaaaatta agtcgattga tgaaagctgc
     3481 attcagactg taccttgtgg agacaaggaa gcagtgtgca gattttcggg attggcttgg
     3541 ttttttcgat gtatgggtct ttttgtgtgt atccaaccct tgcatcctct ttagccagtt
     3601 ctgaaaatag gactggtctt tttttacgag gtgatggaaa atgtctaaaa tcgaaataaa
     3661 aaatctgaca ttcggctacg acagccaagg cacattatta tttgaacaag caaatctaaa
     3721 ttttgacaca caatggaaac taggacttat cggacgaaac ggtcgaggaa agacaacttt
     3781 actgaatatt ctacaaaaca aactacctta tcaagggcaa gtaatccatc agcaagaatt
     3841 tgcctatttc ccgcaacaga caaaagataa agaacgttta acctattacg tgttaaatga
     3901 tattacggat tttgagatat gggaaatcga aagagagctc caattgatgc aaacagatcc
     3961 tgaaatctta tggagagaat tcagcacact atcgggggga gagaagacaa aagtcctact
     4021 ggcactttta tttgtggatg acactcattt cccgttaatc gatgaaccaa cgaatcattt
     4081 ggatatctct ggtagaaaac aagtagcggc ttatttgaaa aagaaaaaac aaggcttcat
     4141 cgtggtcagc catgaccggg gatttatcga tgaagtagtg gaccatgttt tagcaatcga
     4201 aaaaagtcaa ctggaacttt atcaagggaa tttctctatc tatgaagaac agaaaaaact
     4261 tcgtgatgaa tttgaaatgg ctcaaaatga aaaattgaaa aaagaagtca gtaggctaaa
     4321 gaaaacagca gctgaaaaag ccgaatggtc tcgttcccga gaaggagata aaacaaagaa
     4381 acaagtcgga ttcatcgata ctgaatctag acgagtgaat aaaggagcag tgggtgctga
     4441 tgctgcacgg acgatgaaac gatccaaagc aatcgtgaat cggatggaga cccagatcag
     4501 cgagaaagaa aaactattaa aagatatcga atatatcgat tctttgacga tgaatagcca
     4561 agcgtctcac cataagcgac ttttaagcgt agaagatctt caattagggt atgaaaatct
     4621 gttattcgag ccaattcatt ttacaatcga gcctcatcag cgggtggcga tttcaggtcc
     4681 taacggtgca ggaaagtcat ccattatcca ttatcttctg ggggcattca acggcaaggt
     4741 tataggagaa aaataccagc caaaacatct gagcattagt tatgcaagcc aaaattatga
     4801 agacaatcga ggaacgttgg cggaatttgc agagaaaaac caagtagact accaagcatt
     4861 tttgaacaac ctccgaaagc ttgggatgga aagagatgtt tttcataaca agatcgagca
     4921 gatgagtatg ggccaacgga aaaaagtgga attggctaaa tctttatcac agccagctga
     4981 actatataca tgggatgaac cattgaatta tttggatgtc ttcaatcaag aacaattaga
     5041 acaactgatc ttgaacgtga aacctgccat gttactagtg gaacatgatc aaaccttcct
     5101 ggataaagta tctactgaga ttatttctct tgagagaatc taacccttgt aatctcaaga
     5161 acaacaaggt atggttaagg ggaactttgc caacgaagga gagaatcaca tgctttatgt
     5221 aacgagacac ggagaaacaa cttggaacgc acaaggatta gtttgcggac gggcagatgt
     5281 gccgctgact gaaaaaggac agatgcaggc acagaaacta gcagaaaagg tagtagattt
     5341 gcctgttcca atcacgaaaa tcatccattc ccctcttcaa cgagcaagag atacagcaca
     5401 agcagttgct gatcgtctca gcttgccgct gaccgttgat gaacgattgg tagaaatgga
     5461 ttttggggat tatgatggac tgccaagcaa agatgagaac tttcaaaaag cacgtttagc
     5521 gttcgctgta cgattcccaa atggggagtc agtcttagat gtgtatgcac ggatcgttcc
     5581 tttattgaaa gaatgcatcg aagacgaaga aaatgtctat ctgttagttt gccataatgc
     5641 cttgataaga gtgatcaatg cttattttca tccgatgcct aatgaggggt tctttacatt
     5701 catggtagat aatacagaat tggtttctta tg
//
LOCUS       DAGCYL010000165         5586 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.168, whole genome shotgun sequence.
ACCESSION   DAGCYL010000165 DAGCYL010000000
VERSION     DAGCYL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.168"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..242)
                     /locus_tag="MB216_002361"
     CDS             complement(<1..242)
                     /locus_tag="MB216_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527151.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRK"
     gene            complement(229..756)
                     /locus_tag="MB216_002362"
     CDS             complement(229..756)
                     /locus_tag="MB216_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527152.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            complement(779..2116)
                     /locus_tag="MB216_002363"
     CDS             complement(779..2116)
                     /locus_tag="MB216_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBT4527153.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            2236..2715
                     /locus_tag="MB216_002364"
     CDS             2236..2715
                     /locus_tag="MB216_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527154.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            complement(2830..3062)
                     /locus_tag="MB216_002365"
                     /pseudo
     CDS             complement(2830..3062)
                     /locus_tag="MB216_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3065..3382)
                     /locus_tag="MB216_002366"
     CDS             complement(3065..3382)
                     /locus_tag="MB216_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527155.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            complement(3493..3675)
                     /locus_tag="MB216_002367"
     CDS             complement(3493..3675)
                     /locus_tag="MB216_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527156.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            complement(3712..4512)
                     /locus_tag="MB216_002368"
     CDS             complement(3712..4512)
                     /locus_tag="MB216_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527157.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            complement(4649..5068)
                     /locus_tag="MB216_002369"
                     /pseudo
     CDS             complement(4649..5068)
                     /locus_tag="MB216_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5064..5372)
                     /locus_tag="MB216_002370"
     CDS             complement(5064..5372)
                     /locus_tag="MB216_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527158.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
BASE COUNT         1680 a         1196 c          693 g         2017 t
ORIGIN      
        1 aatttcctac tttgttcctt aatttcagca tttcccaatt ctttgtattg agcttcctgc
       61 atagctttca ctttctggtt attcttgact acttcgattt gtttactttt aaggtcttcc
      121 aattctttga cattattatc ataagcattt ttcttttgaa atagtaccac acaacaaagg
      181 atcacaatta tggaaagtat tccgatcgcc tttttagcag tagtgatttc atgattttgc
      241 ataaaaagca cccccagtca ctttcttatc attatttagt gtgatggtta atgtggtatc
      301 atacactttt tttccattgt attgaacttc aaaatcggtc aatgcttcat acaacccact
      361 actagatgtc ttaattccaa agtttgttag cttaaaagta aaaggcttat agtactcgat
      421 tttttctttc tcactactct tatcagtaat tggtggttta cctgttacaa taaatagttt
      481 tctcaaaaat tcttcacctg attgatctgc aaacttattt tgaactgatc cattcaacat
      541 ttcttgaata tcattttcta aatctttccg taatccatcc acctcatttg gtttaacctc
      601 tttaaagaat gtcagcttat cttcaatatg cacactgtgt ttttcagctt ctatgtcctg
      661 attctgctta ttgaagaata ggattccaac aagcagtgct aataacatac cagaaagcaa
      721 agaaatgata attgtatctt tcactttact attcatctat atcgtccttc attccaattt
      781 atttttcgat ttcaaattta gcttttccta caccgctaaa gaaatgcaca ttatctgatc
      841 cgtccactcc atcaatcaac gttaccgaaa ctccccttgt tggagcttgg gttggtggaa
      901 cattaaagtt agcacataga aatgatccat cttcaaacac cgctataact acaccagtat
      961 gccctgcatc tccaacagcc cataaccatc catccgaagc actaaaccca taacctacga
     1021 ttggtttgtt cgttattttt gctcctgccg atttgtagct ttcccataca taaccaccat
     1081 ttccagtagt cggttgattc ccatcgtata attggctcat ataagcccac gtcaactctg
     1141 tacactgacc ttgatatatt ccgttaaaag gtgaagttga ccaatctggt tctttgaatt
     1201 ttggtagcgt gatcgctttc ccatattttt tcaaagcttc ttcataagtc atattcgcta
     1261 ttacttgacc ttttttaccg ttaatttttc cttctttccc aattgatcca gaaccagaat
     1321 tagcgtcatc cgtagttgca caggcatctt ctactgttcc tgatgctgaa cttgatgaat
     1381 tattcgatcc atctccggta cttgaacctc cggatatttt attgatctta ctttcatctt
     1441 ttgcaccgtc catacccttg cttttcatgt acttccagat ttcttttgca ttttcaccac
     1501 gtacagctat tacagctgat tcggaatcag ctgaaatttc ccagttttta tgaaaagtta
     1561 ctgcattttc tacgacatca tcagaagcat ttaaagcata atcttttaat attcctacga
     1621 atcccccatc acctttgaac ataaagtcca attgggcttc tgtagtccac caagaaccgc
     1681 cattattttt cttagaccaa tcaactaatg ctgtatgtcg cccagctgtc cattgaccat
     1741 agccaatacc catcagacca tcagtaaatt gtttgatgaa gtcaacttct ctctgtccat
     1801 ctccgtaata cccttctact gctcgaggat caatacctga ttcttgtttc cagttaccta
     1861 aaatacctgc aatactagca cctgataagc catattctcc attcatgtat ttgtaaatat
     1921 cttcaatatt tttggctaca tcacctgtta cagcgccact tccagtatcg tttccatcgc
     1981 tatctgttcc tgacccacta ccttcagtag tacagttaaa ttgtgcatca taagtagcta
     2041 tcccatcagc atgtatcact tgcggattta ttgtgattac actcattgca atccctgtaa
     2101 attgcaccat taacataagc aatgtggcaa tctttctctt agattttttc aataactacg
     2161 cccccaaaaa gtgtactttt ttatgttatt atgataatat aataacataa attaatagaa
     2221 aggaaaagct aataaatgac tgataaaaca agtgatttac ctaaactagc tccaaaaaca
     2281 tctcgagtaa agagcaaaaa agatggaaaa acaaaaaatg ctgtaaagaa aaagtcagta
     2341 aaagaaacat cctcgttact acttgatatt ccaatgagtt cttttgatga caatacggac
     2401 gaaacttctg ttaaaattgc aactcacgtt ggaatgaaca atcaaaatga ttctttttac
     2461 aacttgttaa acagcctaag agagttggaa gataaaaaaa atcaggtaat cgcatcaaaa
     2521 gctcctgatc tcacaaccga tttggatgct gaaattatcc aaatcaaaca agatgagcga
     2581 tcagttaaat cgctactgaa aacctacttt gatggttttg ataaagaact agctccttat
     2641 agccaagcaa tcctaactaa accaatacaa gctccacata tttcaatttc cctacaaaaa
     2701 aagagtgatg attaaacaat cattactctt ttttagattt ctggtaagcg ctgatcgcaa
     2761 aaaccatcaa caatattgat tcaaaaaata tgttcattcg tgtctctttt ttccattata
     2821 gatttcccac tattccatta aatcgaaagt cacaccgatt actacaaaga ttaacgtgac
     2881 cacattcaaa aacattaaaa gatatgctga taatctcatc atttttttag ttcacttact
     2941 ggtaatctaa acatttgaac gattgcactg gaaataccat aattagcaat gacaaacaaa
     3001 aaatacgcaa taatttcact tacacctaga tagatcagta aaataaataa gtcacttaac
     3061 attgttagtt tcctcccagt atatgatttt catttttggg aagtctttta taatccaatt
     3121 cctcaaaaaa aggaaccata gtgtcactaa tataggtttt agtttcaggt accgccaaaa
     3181 ttccccaatc aagaatttct tcccatgata catcatcaaa ttctaaaata ttatgatcaa
     3241 ctgacctatt accaagttca tatccaccaa agatcaccat attatcaatc atgtatagaa
     3301 tttcatcagg cgaaccatga gttaatgttt cgtcctcttc aagcagttcg tttaatcttt
     3361 gtttgtattc ctctcttttc atacttttat cccttctgac tcattttttt gaattttaac
     3421 atttttaacc caagcatgac tatctttaca tgctattaat ttttatctcc attttgatat
     3481 ttctgttttt ttctactcac caatcaaccg taaatcctca atattctcac cttcaagcga
     3541 atatgaatat acttcgccgt aaatattttc tataactacg acttcttctt caaagtctat
     3601 ggactgcaca tcaaaccatt ctcctccgcc aaattcgtct atgattagta cctgcaatat
     3661 tttatttact gccatttcaa tttcccctta taccattcta tgtcttccaa actatttttc
     3721 ttctttccat accaaggttt cacgtatctc aaaattttct ttaaataagt gttccggtac
     3781 ttttataatt aactcttttc ctacttttat cagatagtct ttttcaccta atgagatttg
     3841 accagtcggt aactgtatag aaactctttt agttttatta aattttttag ttttcaatac
     3901 agtatacggt ctacagaatt tctctaagcg ttcaaatctt ttactatcgt attggaaata
     3961 agctttagga acagataaag tacaaaaact atcggtaaca gcacttatgt cttttttaga
     4021 aattatccca actttagaac ttctaaggaa agttgctaat ttcttatatc caccatgact
     4081 tgtttcaata tagatgctat tgtattggct gtcgtctatc tcatttattg tttcaacctt
     4141 tttcctgaca ggtgtttcaa cgagttctcg aacctcttct tcactgtacg ctaatacaat
     4201 taactcttcc ccatttacca tctttagaac atacgcttgc ttcttttttc ttggcttatt
     4261 ttgagattca acataaccag acatggaatc tttatacttc caagcatgta cttttttctc
     4321 taatttctgt atctcttttt tcagctgtag atattcagac attttttcat tgtaacgttc
     4381 gcaatataaa caatattcat cttttccaaa atcatgatca agtccttctt caatatgttt
     4441 attcaacaaa ccggaaaaat actgatcata ctgcttcttt cgtaacttca gctctcgtaa
     4501 atcttcagac atttcatttt tcctttccaa ttattggtgc catttcacaa tttgggattg
     4561 actaaacacc ataattaatt tgtaagctaa cacaattaca actgttggta attgtgttag
     4621 tctccatcaa cttgtctggt ggcttttttt attgcacaaa tttcatagct ctcaaaaagc
     4681 gcttggcttg aaaaggtgtt attctacgaa ccttattcgc caatttccaa tctgataaag
     4741 caatttgcca ttcgtgcatt tcaaaatccg gcatgatttc tttgatatac tgtagctctt
     4801 tcttttctct ttcttttaag aaacggctca tcgagtttaa cattggaact aaggatgcgt
     4861 aggtacttga ataacttcca atgcgtttcg tagtaattac ataatcaata aaaacactat
     4921 taagaaaggt caagtttata aactctgatt catacactct aaaatttgca gttaatcgct
     4981 tcgccttgtc accccaatag tcacttttgt tgtaggcttt ttcccctcga atatagcact
     5041 ctgtttctct ttcgtaataa tagtcatact tttttcatca tcaaatctga gagtttttga
     5101 ttatcttttt cactgtaaaa taatctactc gttaaacttt taggataatc tttgatagaa
     5161 tatgcatccc aaacaattct ttgaacatat tcacgatcta tattttctct gacttcccca
     5221 gtccaaaaat gttgaccata atggcatctt tctattacat tgttagcaga aacttcaaaa
     5281 taggattgtt ccctttgtat cctcatacca atcatcatag cgcaggaaat aactattaat
     5341 aacaatcctg tttttaatct attattccac atgcagtttc cacatcctta tcttgataaa
     5401 aatcctcttg accctctcca attactgttt taaatataac caccaaaaca agatcatgct
     5461 aagaaatcca agagtatata agatgataca ttctttgtta attatccaaa aaactctgat
     5521 ttctttgagc ttttcacgca ttaggaaaca ccttgttata tcttttttgt atttctttca
     5581 attgtt
//
LOCUS       DAGCYL010000166         5570 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.169, whole genome shotgun sequence.
ACCESSION   DAGCYL010000166 DAGCYL010000000
VERSION     DAGCYL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.169"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(96..317)
                     /locus_tag="MB216_002371"
     CDS             complement(96..317)
                     /locus_tag="MB216_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527159.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(314..604)
                     /locus_tag="MB216_002372"
     CDS             complement(314..604)
                     /locus_tag="MB216_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527160.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(601..1785)
                     /locus_tag="MB216_002373"
     CDS             complement(601..1785)
                     /locus_tag="MB216_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBT4527161.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(1967..3370)
                     /locus_tag="MB216_002374"
     CDS             complement(1967..3370)
                     /locus_tag="MB216_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBT4527162.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3392..4165)
                     /locus_tag="MB216_002375"
     CDS             complement(3392..4165)
                     /locus_tag="MB216_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527163.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4175..4552)
                     /locus_tag="MB216_002376"
     CDS             complement(4175..4552)
                     /locus_tag="MB216_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4527164.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4573..4887)
                     /locus_tag="MB216_002377"
     CDS             complement(4573..4887)
                     /locus_tag="MB216_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4527165.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(5087..>5570)
                     /locus_tag="MB216_002378"
     CDS             complement(5087..>5570)
                     /locus_tag="MB216_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBT4527166.1"
                     /translation="RMYSGDDNGVDLYKFVTKHIFVNLNIDEKNYPSIFKHYLFFINE
                     VEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNE
                     IFNVSDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI
                     "
BASE COUNT         1656 a         1095 c          940 g         1879 t
ORIGIN      
        1 aattaaaaga gatttaaatt gatcatttgt aaacatattc ataaatcctt tcataacaaa
       61 atatatactt cctattttat catgaaaaat atctgttaag caccaatgat tcggttaaat
      121 aagtttaata acacatcttt cacgccagct gcattgaaca caagtccgac ggcgattaga
      181 gcaataatca agaagccaat gagttttgaa aattctcgtt taaaacctaa aaacagtcca
      241 atgacaacaa tcgctaacaa taccaatgac tgtgcattat ccaagaacca gttgtacaaa
      301 ttctgcccaa aattcatgtg attgtcctcc tttccttaag atatttttct cgataataga
      361 tatagctttt tacacgcctt aataattgaa aaggggttaa atgctctcca tagacggtta
      421 ctcgattggt ataattatta taaaatctca ccttgtaaaa atcataggta tagatggtat
      481 caccagcttg ccgttttccc tcaataatga gagacgtata tttccctgta ttttgtatat
      541 acgcttgaaa ctcaatgcca cactgaaacc ctaacttctt taattctttt gttaaaaggc
      601 tcatgtaatc aaatcctcca tggctagatg ttgttgttca attaatttct catgtttttc
      661 tgtcagtttg gcttcctgaa tcatggtgga gataatggtt gtaccgttga gaacatctaa
      721 aatgcttgcg atttttaatg taggggcgac ttgatgatgt aaccagttca atgttcgttc
      781 aaaagaataa ggctctggct gtgtggttag gcgtaatgct ccacgatttt taccgataaa
      841 ccattcccaa cgttcattag ttttccaatc actccgacgt ttagtgtcgt ctttatctgc
      901 aaagcggata tagcgattga taatttcaaa ggctgttttt tccgcctgtt gatgtgccaa
      961 taaatctttc atggcttggg tggctcggtc attttttaac cgaatttcaa accgattctt
     1021 tacttctgcg tcttctaagg gaacatcatt tttcacgtat tgctcataat ctttctcata
     1081 tatacagaaa tagacttcac tttttaatga gccaatataa agtgtgtttc ccatgtatgg
     1141 tttttcatca cggtgaacca attcaccact gcgataattt ttaaagcttc gaaagacgga
     1201 aatgcattct tcctgtttac atttctttgc caaatctgga atattcaaaa tccctgtttt
     1261 atcgttgata gctaaatcca agcgtttgaa aataccttta tgttccatgc aatcttgaaa
     1321 gaaatcatac cagctccgat gttgagccaa tagaaagttt tcaaattgcc gacagccttt
     1381 tcctttcagt tctaataaaa cacctttact tacatcatga gagactagga caacaatatc
     1441 accgaaacga tagtgttcgg gataagaata atagccaaag tcttcgtgta gcatataatc
     1501 catattcaaa cgtaaaacct caccgataat ttgagccaca tcattggtag ggaatcgaat
     1561 ccgtacatag tcgaataaca tctccaatgg gttatcggga ttgaagcgtt ccaaagcgtt
     1621 cagtaaatct tctttcacct gttgagtagg gactgccttg cctgtttcaa tatccgatag
     1681 gtaaggtctg gtaatatgag aagcaaccgc cagtttattt tgagaaacgc cataagccag
     1741 tcgcttttct ttcaattcct taatccaaat tttttcgttt tccattcgca aacctccaat
     1801 ctaaaaaaga tgtaaactat gacccttgag tttacacctt tcatattttc cgcaatcctt
     1861 tgtaccatca acattggttg atgagaattg cctgttttca gttgtatttg tgcccccctg
     1921 ttagatagag ggggctaaag gattggcgtg gctaacgcca caccaatcaa agcaagtctg
     1981 aacctgtacc tttcacttcg tgcggtacag gctcagattg ctttttttga atgacttttc
     2041 ctatttctcc taagaaatcg tacctttttg gtacaagggg tgtataaaac tcactaatca
     2101 cactttttcc tgtatccacg taacctcttc ccttgatggg ttttaagaaa aattgttttt
     2161 gtgtatcgct tccgaacatc ataccaaagc ccatttcaga catacgaccc aaagccacac
     2221 ggaagttaaa gttgtcccga atcccatcac taaaatattt tgcgtctgga cgttgacagg
     2281 caagaattaa aaagaagcct gcttgtcgtc ctaacattac aatttgtttg agtttatgaa
     2341 taacggtggt actttcacga cctagcatat ccatcattgc cacatattca tcaaaaataa
     2401 gaaaattggc agacaaacca agataagcat aattttctcc tgttttatag ttagggtgtt
     2461 gtttcatggt ttcactgcga tcaatcatat cttcgtaaaa ttgattgata catgccaaca
     2521 tatcttcttt acggtaatag acattattca tgaccgcccc taagtctgcc aaatcagcat
     2581 ttttagggtc aagaatataa agctttgcat ttgtatgcag gagagattca atcaatgtga
     2641 gaataaaata ggattttcca ctaccagtgc ccccagcaat taacatgtga gggagagaat
     2701 cgaactccca atagaccgat tccatcaatt tgacacgtcc atcttgagct accacgtctt
     2761 caatgccaat tcgattgcca atagtatcat aaagtaggac atactctaca taagaatctt
     2821 tcaacgtttt ttccactaat tcacaataca atcctgtttc tagtttgttt tctaggtgca
     2881 aaagctgatc ttgatatttc cctaaggtga tttctacatg aatatggatt agtccatctt
     2941 tcattcgata atatatttta ggaaaatggc ggattcgttg tttggttttc ttgctgggta
     3001 aatctttaaa gaatccctct gattgagttt cttcggcttc ataccaatga ttttctaaaa
     3061 tcatacgagc taacttttga cggtgctcta actgttttat agaatctata cgatagtgat
     3121 aataaagcca gctgataagt ccgcatacaa aaagagcagt gagaatactg ctccaaaagt
     3181 atggcgtatg aagttgatag aaaaatgtat ctaactgaac tgcttgccag ttcaccgatt
     3241 gtaacgctct ccaatgaaaa gggagtagga cgactagaaa aactaccagc aatgaaatta
     3301 gtacaaaatg atagaccaag tgcttatcgt tggctttgat tcgctctcct ctgtcatgaa
     3361 gcaatggcat aaaaatcctc ctttccacct atcaaaataa ttgtgtattg tagatacgag
     3421 cctgtttatc aaaagttggt tgagaaatgg cttcatattt ctgataagcg attcgctttt
     3481 caaagcgtaa atttttttct ttcggataat aatgacgtcg atagagagtg tgtcctaaca
     3541 gttttttcgc ttcttttgtc aacggataga gatagcgaaa catgcgtcca ttgattttct
     3601 caatcccttt gtattcgcaa aatgcctggg aaagccaatg ctttttctct acctgttcaa
     3661 agcgagcatt ttcctctaat aaaaggcgag cacttcgagg gtgtatcttt tcccatgact
     3721 gcttatcacg atagacactt gtcttgaaat acccgcagta aaagaagttg gacgcttgat
     3781 agacatagcc acattttccc tcaattccat ctgccagtgt atataaaaaa tgacagtccg
     3841 tatgttcttt caaccactta attagagctg aaagagcttg actgccaaaa taattggttt
     3901 gattcatttc tggaagaaaa cacatctttc caatttctaa ataatctgtg gtttgaaggg
     3961 aactgtcggg aaagagtttg cgtatggttt gtaggggttg cgtaccccag cctaattcca
     4021 caacgcctaa taatttttct tctgaaaaaa tccctagaaa gtatttacac aatctaggga
     4081 gtatctttga ataatggtat tgccgaataa aagaaatggc ttcatccttc ggaatttctt
     4141 tcaaaatcaa tggtgtgttt gtcataagca acacctattt ttcactttcg gacgcttctt
     4201 ttttatattt ctgatgaggt tttgtctgtc cttttaatac aagatcgtct gctttcacgt
     4261 accaatcaac gtctgcccct cgaaaagtag ccgtcgctac ggtatctgcc acaggattga
     4321 ttaattccac ttctgcgttg tagtcaaatt ctttcaaagg aacagaagcg gggatactga
     4381 cttgtatcat gcgtccttgt ccttttgatt ttaagtcata ggtacgttct ttaatttctt
     4441 cggaaaccgt accatcttca ttctggatac gaacctcacg acgcaaggcg gaaaatttca
     4501 atgtgccaaa tgtctggtct ttttcaatca caataccttc tgctaatctc atattttttc
     4561 acctttcctt tcttatgctt tcaccatatc gtctgcgtgc ataatgtaat tggtaaatcc
     4621 tcgattgcca attttataac cttctgcggt aatatgtggg ttgatgagtt tgactttttc
     4681 gtcggattca aaatgtttct cgccagcttc ggagggaaga atgacaacaa tatcatctgc
     4741 tcgttgaatc gttgagtata aattgtaact acgagagaga atggttggtc tgccatttat
     4801 tcgacgttgt tcaatgttcc cttcaccagc atattctaat tgtccaaatg ttttttccat
     4861 attgggaatg acatatttaa gttccatatt tttacctatc ctttctgttt ttatgtttga
     4921 atgaatctta tttgtgtgat ggttttaaag gggggagagc gaccttttga ttcttgattt
     4981 tttattttca taagttcact tcctttcaaa attgggtaaa aaaatagacg ctccgtactg
     5041 agcgtccgat tcctactata tttaaaacaa cgactaccta ggtattttaa atattataat
     5101 caaattctat tttatctaat aattgagtgg gataaagcaa gtctccttta ctcgtaaaat
     5161 aacccacctc tgagaaaaga aataaattat tttttgctct tgaacaaatt acgtatagta
     5221 gttttctcgc tgaactaatt cctttatcag aaacattaaa aatttcattc caatgaggaa
     5281 ttttcccgtt tagaagatta atagcgataa cagtatcata ctcatttcct ttaactttat
     5341 gtgctgtagt aagtgtgata ccatctcgag atttaaacat cttcttaaaa ctagatacac
     5401 tactagataa tgaattattt tttatccgat cttctacttc gttaataaaa aatagatagt
     5461 gcttaaaaat agacgggtaa tttttctcgt caatattaag attaacaaat atatgtttgg
     5521 taacaaattt gtataaatca acaccgttgt cgtctccact atacattctt
//
LOCUS       DAGCYL010000167         5388 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.170, whole genome shotgun sequence.
ACCESSION   DAGCYL010000167 DAGCYL010000000
VERSION     DAGCYL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.170"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            157..1089
                     /locus_tag="MB216_002379"
     CDS             157..1089
                     /locus_tag="MB216_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527167.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1310..3073
                     /locus_tag="MB216_002380"
     CDS             1310..3073
                     /locus_tag="MB216_002380"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBT4527168.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3157..3615
                     /locus_tag="MB216_002381"
     CDS             3157..3615
                     /locus_tag="MB216_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBT4527169.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3724..4407
                     /locus_tag="MB216_002382"
     CDS             3724..4407
                     /locus_tag="MB216_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBT4527170.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4554..5168
                     /locus_tag="MB216_002383"
     CDS             4554..5168
                     /locus_tag="MB216_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBT4527171.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5184..>5388
                     /locus_tag="MB216_002384"
     CDS             5184..>5388
                     /locus_tag="MB216_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527172.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1915 a          845 c         1109 g         1519 t
ORIGIN      
        1 gtattggatt tttttaaaga aaatgattat ttgaaaaata aagaaatcat tatcccagaa
       61 tttgactcgc ggaacatctt gctagcttta gtacgttatg tcaattggga aggctatgaa
      121 aataaaaatc cgtatgtaga agaagcttgt aacgagttga ttgattatgt agagactcat
      181 ttttttaaaa gaagatattc ggaagatgaa aagtccttta tcatacgagg aatagagatc
      241 gcaatcggaa gaaaggcatt tccccttgga ctgaaagaag aagataagaa aagcgcagaa
      301 acgaagccgt tatttcaatt agtctgtcaa ggattggaag agcaaaaagg aattctagac
      361 ttacaagaag aagatgtcta ttatattttt tctttgttca acagtagaaa ttataccaat
      421 gagaacatgg agctgttaag aaaagatttc gaagttgttt ataaaaattt tgttctcaat
      481 gacctatcac tgaatgaatt gattttacag attattagta tcgctgaaat cgacgatgca
      541 gaaaatttta ttttcaagca atccttttta caatttgtcc gtacgttgtg ggcagatgga
      601 caagtatttt taccggaaag gatctatctt ctatcagaaa aagaacaact tctgtacgga
      661 aaaattgtag aaatattgga agagtggaaa aagaaaaatc aaatccaact aagatggaat
      721 gaaaatttca ttcgaaaatt cgtcaaaagt ctttctttga tcaacagtga acaagcgggg
      781 agacaggaaa tagaaatttt tgttgtttca gaaagttcag tcaaacaatt tttttatcga
      841 acccaatttc atttgtatgc ggaagaaggc atcgctgaaa taaatcctat tttatttcgt
      901 tcattagaag aattgcctga tgaatgtcta tgtgcgaaaa aaagagtggt tttgtgcgat
      961 gctgctagct atcaaaatgg attagaaact gaaaagacaa agatctatcc aatctcatta
     1021 aaaggtgcaa acatccattt gtatcatatt gctcaggaac ttcacctatt aaaaaaacat
     1081 atttcttgat gagaccttaa ttaggagacc ttctacaaag ctttttaatt gtctgtagaa
     1141 ggtttttatt tgagtcctga ttcgtgcgaa gatctactta tataacaagc tttctctgaa
     1201 atttttttaa aatgctattg acttagagtc aactctaagt agtagaatag ataaagtgta
     1261 acaaatctat tttcattaaa agaagatagt acggaggaga gaatgtatta tgagtacagt
     1321 attacatgta ttggatgcaa tcaatagccc cgcagatgtc aaaaaattat ctgttgagga
     1381 aatgaaacag ttggcagaag agatgcgcca aaaaatcttg caaaaggaca gcgatgtagg
     1441 tgggcacgtt ggtccgaatc taggtgtagt agaattaacg attgcgtttc attatgtatt
     1501 tgattcacca aaggataaaa tcgtttggga tgtttcccat cagtcttatg gtcataagct
     1561 tttaactggt cgaaaaaatg gttttgaaaa aggacattac cgtgaagtga gcggatattc
     1621 caaccaacat gaaagtaaac atgactattt tacagtgggg cacacatcta catctatcag
     1681 cttagcaaca ggtttagcta aagcgagaga tctgaagaaa gaatcaggaa atgtggtagc
     1741 atttatcggt gacggctccc taagtggtgg cttagctttt gaaggattga acaatggtgg
     1801 tgttttacaa tccaatctaa tcgtattagt aaacgataat gagatgtcta ttgatgagaa
     1861 tcatggcagt ttatataaaa acttagctga gcttcgtcaa accaacggta aaagcagcaa
     1921 taacctattt aaagcaatgg gatttgatta tcgttacgtg gataatggaa acgatctgga
     1981 aacggtgatc cgtttatttg aagaagtaaa agatattgat catccgattg tacttcacgt
     2041 ccatacggaa aaaggtcgcg gttatcagcc ggcaatcgat aataaaatga aatatcattg
     2101 gcatgtacca tttgatctgt ccaccgggga ggcaaagcaa gtaacttctg gagaaacatt
     2161 taatatgatc atcatggatt atttggataa gaaaatgcaa acaagtacag ctcctattgc
     2221 cgcagtcaat gcagcgatac caggcgcctt tggactgaaa acattccaag aaaaacatcc
     2281 agatcattat tttgacgtgg ggatcgctga acagcactct atcactttcg cttctgcttt
     2341 ggcttctcag ggagtacgtc cagtcgtctt ccaatcaagt actttcttgc aacgggcata
     2401 tgatcaatta tcacatgatt tagcgatcaa cgagaatcca gtgattattt tagtcggtgg
     2461 cggaacgatc tcagctagtg accgaacaca ccaaggaaca tttgacattc cttacttatc
     2521 aagtatcccg aacatcaaat atcttgctcc agcaacaaaa gaggatatga tctctatgct
     2581 agattgggcg ttcgaacaaa cagcgcagcc tgtagcaatc cgaattccta cacatggtgt
     2641 agaacatggt acagctatcg caaatgatta tagtattcca gcctatcaaa cagtaaataa
     2701 tggaggaaaa gtagcaattt tagctttagg cggttttctt gaattaggaa aagaaacagt
     2761 aaaagctttt agaaaaaaag gaatcgaagc aacgttgatc aatccaggaa cgatcacaga
     2821 aaatgacgta gaaacattag ataaattaaa agaaacacac gaattagtca tcacattaga
     2881 agatggtagt ttgtcaggag gatttggcga aaatatcagt cgttattatg gtgattcttc
     2941 aatgaacgta ttaaattttg gtgcagagaa agaattcacg gatagcgttc ctgtagggca
     3001 gctttatgaa cgttatcatt taacaccaaa acaaatcgtt gaagatagtc tgcgtgtctt
     3061 gaatattcaa taattcatta tgctcaccat ccagataatt gtttatacta gtatctggat
     3121 ggttttttca ttataaaaag caaaggggtg gatgttatgt atcagcaagt agaagaatat
     3181 gtcaaaaagt tccaagaaaa atatcctagt tatcaatcag aaaaattagg tttcttttca
     3241 tttggggata cagaaaaaat ggcagacgag ttgagcgaac tagttgtaaa aaagataaag
     3301 actggtacat caagtggtta cgatctgtat aaacaagaga atgagccttt accaaaagtt
     3361 ggtcaattag acgtggtcct gaatggaaaa aatgaaccag tttgtatcat caaaaatata
     3421 aatgtgacca tcttgccttt tagtgaagtc acgaaagagc atgcttggaa agaaggagaa
     3481 ggggatcgga cattagctta ttggcggaaa gtccatgctg agttttttaa acatgcttat
     3541 aaagaagcgt tgaacataga atttacagaa gagaagctgg tcgtatacga agaatttgaa
     3601 gtaattttct tttagtaaga aaggtctgct gaaagaaata gctcattatt gtctcctgaa
     3661 tagaaaaggg ttacattttt atatgaagct gtatttttat ataacattac tggaggagat
     3721 attgtggata cactggaaca aatcaaaggc ggagtcgtcg tttcttgtca agctttggaa
     3781 aacgagccgc tacacagttc ttttattatg gggagaatgg ctttggctgc aaaagaaggc
     3841 ggagcagttg gtattcgagc aaatacgaca gaagatatca atgaaatcaa gcgaatcgtt
     3901 gatttaccgg taatagggat tatcaaaaga gattacgatg ggtcagaagt gtttattact
     3961 gcgacagaaa aagaagtgga agaacttttg gagacgcaag cagaaatcat tgcactggat
     4021 gcaacttctc gtaaacgtcc gacagaaaaa acaactgctg aacttgtcca aatgattcac
     4081 gaacacggtc ggttagctat ggcagatatt tcgacttttg aggaagcgct tcaagcgcaa
     4141 gaagatggtt ttgatctttt gtctacgact ttagcaggct atacagaata ttcacgaaag
     4201 acgcagacac ctgattttga tttattgaat caaatcatag aacaagtcga tatcccagta
     4261 attatggaag gacatactga ctctcctgaa caagtagaga aagcatatga attaggtgct
     4321 ttttcagtcg tagttggatc tattatcacc cgtccgcaat tgatcactaa aagatatgtc
     4381 gaagccgcaa gtaaagcaaa taactaaaaa acacgcacgg cacagattct tgacaaaaaa
     4441 gaaaaattga tagatagttt aaagcagatt ctaagaagat gaaaaaagca ttacttttca
     4501 ttttgtcttc aagatctgct ttttaacgaa ttttatagaa agaaggcgaa accatgtttg
     4561 aacggagtcc ttcagaattg acagaacgtg aaaattacaa gttgctgatt ggttcagtca
     4621 tccctagacc agtagcagta gtaacgacta aatcagcaaa agatatagtg aatatcgcac
     4681 ccttcagtta cttcaatatc gtcagttcga atcgacccat cttatccttg gcgatccaga
     4741 gaaaacaaga tgcagtaaaa gacacagcaa agaatattct tgagacaaga gaagctgtta
     4801 tccatatcct tgatgaggac aacgtagaag aagtaaataa aacagcagcc aatctgccta
     4861 atgaggaaag cgaactgtac ctgactgact tgactttaac tgattcagta atcatttcag
     4921 taccagcatt gacagaagca aaagttcgtt ttgaaacatc cttatatgaa catgtcgaga
     4981 tacggaataa aaataaagta acagctgatt tatttttgct agaagtacag aaatatcatt
     5041 taaatgaaac agtctatgat gataagacag gaaggatcga tccaagaaaa ttgaatgctg
     5101 taagccgttt ggcaggaaat gattatgcag caataggaga aattttcacg attgcacgtc
     5161 ccgaataaaa aggagcacta gttatgttaa aaataagcta taaaccatcg acagattcaa
     5221 aagaaatgaa aaaagaatat gaaacagtga atgattttct gcaagggcag tacttagaag
     5281 ttccgccatt gcaagaccat tttgtcgtta ctactgttac tttagatggt aaggaaatag
     5341 aaatgccaga tcaaacgatt agcggattgt ttaattactt taacaaat
//
LOCUS       DAGCYL010000168         5336 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.171, whole genome shotgun sequence.
ACCESSION   DAGCYL010000168 DAGCYL010000000
VERSION     DAGCYL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.171"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            96..376
                     /locus_tag="MB216_002385"
                     /pseudo
     CDS             96..376
                     /locus_tag="MB216_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(454..987)
                     /locus_tag="MB216_002386"
     CDS             complement(454..987)
                     /locus_tag="MB216_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBT4527173.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1081..2259)
                     /gene="mutY"
                     /locus_tag="MB216_002387"
     CDS             complement(1081..2259)
                     /gene="mutY"
                     /locus_tag="MB216_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBT4527174.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2252..3049)
                     /gene="recX"
                     /locus_tag="MB216_002388"
     CDS             complement(2252..3049)
                     /gene="recX"
                     /locus_tag="MB216_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBT4527175.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3156..4538
                     /gene="rlmD"
                     /locus_tag="MB216_002389"
     CDS             3156..4538
                     /gene="rlmD"
                     /locus_tag="MB216_002389"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBT4527176.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4712..5290)
                     /locus_tag="MB216_002390"
     CDS             complement(4712..5290)
                     /locus_tag="MB216_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBT4527177.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         1540 a         1087 c          960 g         1749 t
ORIGIN      
        1 aaattctttt attctttttt tatttttgaa aacttgccat actttttatt tgttttattt
       61 tccagtatac tatacgaaag gaaggtggct agaccatgaa agttgtacaa acaaaagata
      121 caatgagcaa tatttactta gatgctgtac ggatcaggca tcaagttttt gtagtcgaac
      181 agggtatgcc tttgtctcga gaaatagaca aagatgaagc ccattgtatc cacttcgtcc
      241 tgtactcaga taaaaaagaa ccgcaaggaa ctgtacgtct tttgccttta gaaaacggaa
      301 aaatgaaatt acagcggatg gcgatccttt tgaagatgct ggcatgcctc atatttatat
      361 gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa aacagcaagc ttttgagaaa
      421 aatctctcac tggcttactg ttcttttttg tgtttactta cgcgacaatt gcttgtaacg
      481 gttataccaa atatcaatat agccttccga gaacgggcca tctccattat tgatccagtc
      541 aaccagaatc ttcacatttt cttttaggat gaagtcaata tcgttcggat aattcatcat
      601 tttgctgtgc atctcatatt catcgacatc caatagtcgt ttctcgccgt caggaaatac
      661 cttgatatcc aagtcatagt cgatatattt caaggcctca tcgtccagca aaaaaggtga
      721 agctagatta caataatagg aaaccccctt ttctctgatc attgctatta cattgaacca
      781 gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt acccatcggc gtccatcaga
      841 ttcagtgact aatgtgtgat cattcacgcc gatcatagag tgttcgcttg tttttaatac
      901 catggtatct cgccacgttc gatgcaattt gccgtcgtgc ttgtaacttt ggatcgtcac
      961 aaactctcct tcttttggaa ctcccatagc aacccttcct taagcagcat tttgtgcttt
     1021 gtgtttgggt gttcattaca ccgacatcat aattattata tcaataaaaa tgagctttgc
     1081 ctagaaagga ttcctattgt ttcgattttt ctctaactga tctaccagtt tcatttggac
     1141 cttaggaaaa acgaccgaat caaatgcatc aggcttcaac cacttcgaag ttttgttctc
     1201 ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa aaaaggagaa catgccattt
     1261 cagatgacta aaaatatgag taacttctcc taaatgtcgt gtctgccaaa caaagagttc
     1321 ctgcttttca aatgggagat tttgctcttc ttctgctact aggtcatcaa acagatcgat
     1381 ttctggcttt gtttcccatt cttttttcaa tcgctcatac tcctcttgtg tcacttccat
     1441 cattggaaat gtccacatat tcgccagcaa tttttggcta tctctttctt caaaataata
     1501 cgctccagaa tgattttgaa gagccgcact gatataataa acgtcttttg gctttgcttt
     1561 ttttgttttt acaggaaaac tcgtttggat tcctcgtttg ttagctaaac aaaaggcttg
     1621 gatcggacaa gtctcacact ttggagaagt tggtgtacag atagctgaac ctagatccat
     1681 cattgcttgg ttgaattcac ctggatgctt ttcatcaatg attttccgca tcgcttcatc
     1741 aaagattttc ctgctagaag cttttgcgat gtctgcttca atgcaaaaca atctgctgac
     1801 tactcgcatc acgttgccgt caaccgcggg ttctggaagt ccaaatgcga tacttgcaat
     1861 cgcgcctgtc gtatatggtc ctattccctt caatgagcta atttcttcag gcgtttgcgg
     1921 catttcccca tcaaattctg acatgatctg tttagcagca gcttggatgt tccgggctct
     1981 tgagtaataa ccaaggccct cccatgcttt taaaagcttt tcttctggag catttgctag
     2041 ctcttcaatt gtaggaaacc attccatgaa acgataaaaa taatcgatca ctgtgtctac
     2101 tctggtttgc tgaagcatga tctcagagat ccagatacga taaggatcac gattatagcg
     2161 ccaaggcagg tttctttttt cttgttcata ccattgaatg aactgatctt gaaattcttt
     2221 tgtttcttta tctgtccatt tttcccattc tttattcact ttcctcttcc ttttccgtaa
     2281 tgaatcgctg gatcatatcc aaatcaaatc ctttttgata taagctttgc tttactttta
     2341 gatttctttt tttaggttca aaccttaaat ttcttcgcca tagtttatct ccttgtttga
     2401 ctaaataatc atattctatt tcttgatctg cttcttgcgg aagatcagcc atcgcttgtt
     2461 ggatcacttc ttgagaaaaa cctttcgtca tcaatgtttg ttggattttt ctcaataatt
     2521 ctttttggct ttttccgcga ttttttccaa acgctttttc ggctgtctga tgtgcatttt
     2581 gaacttggtc ttcaaatgta tattggctga gcgcttgttc aatgatttca ggtttcaaac
     2641 ctttttgctg catctgctgc gctaatttct ttggtccttt gtcacttaag cgcatattcg
     2701 tgcgcacata actttcccca tagaccaaat catcgacaag gtccaattct tttaagcggg
     2761 ctacgatttt atttcgatct tcttgaagga tttccttttc ttttaagtat gttcggattt
     2821 ctttttccga tcgcaactga taacttaagt aattcattgc taggtgaaaa ccgaaatcag
     2881 ctccgctact cttcttgatc tcttggatag tttcttcgtc taattcccgt ccttttaata
     2941 atcgatagcg tactaataca tcttcagata cttgtaaagc ttctccttca gataaatcta
     3001 cgcgatagaa agggcctttg cctttactga ttctcgtaac ggttaacata aacatcctcc
     3061 tcgaacatat attctctatc ataccacaga aaatccccgt aatcaggtat tatatgttag
     3121 aataaaccac agaaaaaaga aaaagggaga tacttatggc agaaacaatt gtcaaaactg
     3181 gacaaacggt tcctttgaaa atcaagcgtt taggcataaa tggtgaagga atcggttatt
     3241 tcaaacggtt gatcatattt gttccatatg cactaccaaa agaagaagta ctagtgaaaa
     3301 tcacgaaagc aactcctcgc tatgcagaag gagaattaat aaaagtcaaa aaaacaagca
     3361 aagatcgcgt agtggcacca tgtcctgttt actatgaatg cggtggctgt caattacaac
     3421 acttagctta tcaagctcaa ttggatttta aaaaggatct gcttttgcaa gcattggaaa
     3481 aattcaaacc agcaggcttt cgatcttatc aacttttgcc gacgatcggg atggatgagc
     3541 cttggcatta tagaaacaaa gcacaatttc agttacgaaa aaataagcag actcaaaaaa
     3601 ttgaagctgg tttatacgaa gcaaactctc atcagttagt ccctataaca gactgcttgg
     3661 ttcaagaacc tcgaacacaa aaagtaatga atactgttgt tcaactattg aataaatttg
     3721 atgcacccat ctatgatgaa cggacaaaca gtggaatctt ccgcacaatc atggttagaa
     3781 tcggtgttaa aactggggag ttacaagttg tttttattac tcgcagtaaa aaattcccgc
     3841 aaaaaaacgc aatgatctgt gagatcaatg aacgactgcc cgaagttgtt tcaatcatgc
     3901 aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga tgaaacgatc cacttatggg
     3961 gaaaagaagc cattgaagaa caaatcaatg aagtgacttt cgatctttct cctcgtgcct
     4021 tctttcaact caatcctcag cagaccgaat tgctttatgg cgaaggaatc aaagcattag
     4081 atgtccagcc taatgaaacg atcgtggatg cttattgcgg agttgggacg atcggcttaa
     4141 gtgtagcaaa acaagccaaa gaagtacggg gaatggatac cgttcctcaa gcaatcgctg
     4201 atgcaaaaaa aaatgcggaa agattaggat atcacaacac tcgatatgaa gtcgggacag
     4261 cagagcaagt tctgcctaaa tggttgaatg aaggttttca gcctgatggt atcatcgtag
     4321 atccaccacg tacgggtttg gataacgcat tgatccaatc attattgaaa taccctccaa
     4381 aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc cagagatctg gttaatttaa
     4441 gtaaaaagtt caagattgaa tatttacagt ctgtcgacat gtttcctcag actgcacgtt
     4501 gtgaagttgt cgtgaagatg acgagactcg ctgactaaat gaaagaggtt gtgaaaaagt
     4561 ctgccctgta tcaagtaata agaacagcca aacaaaaata gctcttcatg ttttatgaat
     4621 accgtacttg tgaccgaaga gttaacagga ggacactatt tatttaaaaa aagggttgtg
     4681 acatagtttt tgtcacagcc ctttttgatt attattggat attcacgtct atcacttgat
     4741 aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa aatcacatta tatccttttc
     4801 gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg tggaacagtt gctttgtcgt
     4861 ctatttttgt gatcaattca aaatcactaa atttcaaagg ttgattagga ttccaagttg
     4921 gtttggtaat gtagtaatcc caagtacttg tccgatgttg tgctgttaaa ttccaagtaa
     4981 tatttaatgt tcctggatta accatgtttt tatgccatct gttagatgtc tgttcatcta
     5041 aaggctcaaa gccgctgact cctgcactag caatttttcc atcaatgaat gtatttttgg
     5101 tagcttcgat actttgcggt tcccattgtg ctcttccgac attttgattt aagtttcctg
     5161 ctgctgatgt tcctaggtat gcacggcttc ctggagaagt aatataacca tgcgcaaaac
     5221 ctggaatgct aaaaccgaac aaccctaaac taactaaccc aataaacaat aacgtactaa
     5281 tctttttcat gtagaaacct ccttcatttg ataaccacat tgtaaacgct ttattg
//
LOCUS       DAGCYL010000169         5154 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.172, whole genome shotgun sequence.
ACCESSION   DAGCYL010000169 DAGCYL010000000
VERSION     DAGCYL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.172"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            8..649
                     /locus_tag="MB216_002391"
     CDS             8..649
                     /locus_tag="MB216_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527178.1"
                     /translation="MIERSTFLYVQLQENKTLLHFLPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="MB216_002392"
     CDS             708..1148
                     /locus_tag="MB216_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527179.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="MB216_002393"
     CDS             1160..1828
                     /locus_tag="MB216_002393"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBT4527180.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="MB216_002394"
     CDS             1892..2515
                     /locus_tag="MB216_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBT4527181.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="MB216_002395"
     CDS             2571..2957
                     /locus_tag="MB216_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBT4527182.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="MB216_002396"
     CDS             3170..4561
                     /locus_tag="MB216_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBT4527183.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAVAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1707 a          860 c         1085 g         1502 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt ttaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctgttgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agat
//
LOCUS       DAGCYL010000170         5149 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.173, whole genome shotgun sequence.
ACCESSION   DAGCYL010000170 DAGCYL010000000
VERSION     DAGCYL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.173"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            223..975
                     /locus_tag="MB216_002397"
     CDS             223..975
                     /locus_tag="MB216_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4527184.1"
                     /translation="MGKGIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPI
                     EFMDFAEEVIELITARISSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLY
                     PNEYDIAIEVVERINHRYQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTIL
                     SHLEKSYSNVYDKESLTYHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCT
                     ALKIKDIIEQQFQLAIQENEIIYLAIHLARIY"
     gene            1079..1843
                     /locus_tag="MB216_002398"
     CDS             1079..1843
                     /locus_tag="MB216_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBT4527185.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            1830..3272
                     /locus_tag="MB216_002399"
     CDS             1830..3272
                     /locus_tag="MB216_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4527186.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3283..4613
                     /locus_tag="MB216_002400"
                     /pseudo
     CDS             3283..4613
                     /locus_tag="MB216_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            4626..5084
                     /locus_tag="MB216_002401"
     CDS             4626..5084
                     /locus_tag="MB216_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4527187.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1745 a          754 c         1094 g         1556 t
ORIGIN      
        1 ttatgtaggg tgctactatt caattaggca cgacctaaga aagggttctt tcttaggtcg
       61 ttttttgtct ttaaaaattg tttggagaca aaaatatgta tgaaggagtc tgcaagttgt
      121 tttgttttat catatagtgg aggtgaatta acggtgtatg tgatgaaaaa gctgaatcat
      181 aatgctgtat tgatcgatga cgatggaata gaataaattg cgatggggaa agggattggc
      241 ttttctgcta aagtgagtaa gccatttgat gttgaggatg ctgacaaatt atttgtatta
      301 gattctaaag aaaagatcaa acgattcagt gagatagtct cgcagattcc aatcgaattt
      361 atggattttg cggaagaagt gattgaactg ataacagcta ggatctcctc tccattagat
      421 tcaaatatct atattacatt gacagatcat atttattttg ctattcaacg aaaaagagaa
      481 gatagagatg tcacagcaat tatgttgcca gaaatgagat tcctttatcc aaatgaatat
      541 gatatagcaa tagaagtggt cgagcgaatc aatcatcgat atcaaaccga gttagggtca
      601 aatgaagtag gattcatcac tatgcatatc attaatgctg agctaggaga gcctaatagt
      661 ttaaagagct taaaaatagt agaaatgacg accacgattt tgtcccatct agaaaaatcc
      721 tattcaaacg tttatgataa agaatcactg acttatcatc gcttaatggt tcatatcaaa
      781 ttcctggtta aacgcttgat ttatcaggaa gaaacgccag tagaaaatat tggttttttt
      841 actgaaacat ttaaacatag caaagcgtat tgtacagcac taaagattaa agatatcatc
      901 gaacagcaat ttcaacttgc cattcaagaa aatgaaatca tctatttggc aattcatcta
      961 gcacgaatct attaaaataa gggcgtaact atctataggc gcaaccaaat gacacatagt
     1021 aaatggacta tgattatttg gttgcgccta ttttagtaga aaaaagaagg agaatgaaat
     1081 gggaaaagtg atttttaatt cggatgattt tggctatagc catggtgtga attatgggat
     1141 tatggatgct tatcagcgag gaattttgac atcaactaca ttgatggcaa atatgccggg
     1201 tttcgagcac gctgtgaaac taagaaagga aatgcctagg ctaggtgtag gagtccattt
     1261 aacattgacg tgtggcaaac cacttctcaa aactgtcgat actcttatcg aaagcggaga
     1321 gttcagatcc ttatcttatt atcagcatcc atttaacatt gacaaagatc aactgtatca
     1381 ggaatggaat gctcagattc aaaaaattta tcatgcgggg atcataccaa ctcatttaga
     1441 cagtcatcat catactcata ctttggatga aaaccaggag gtagttgtta cattagcaaa
     1501 aaaatatgat ttgccggttc ggagcaactt tgaacgaaaa gatgaagttc gtcatgtcga
     1561 ttattttgag ccttgtttcg atgtcgtagg agaaactgat gaaaaaaaac gacaaatcaa
     1621 tacgagtgtg gaagaatatt tggcaagttt actcatgata ttgagagaag ataaaacgat
     1681 cgaagtcatg tgtcacgcag cgtatttgga tcaggaattg ttaaatggtt caagttttgt
     1741 gtatcctcgt actcaccaag tcgaattttt aattcattca gcatttgctc aaaaagtgaa
     1801 aaatgatcct ttgattgagt tagtaacata tgaggatatt taatagagga ggaataaaaa
     1861 tgaatgttaa atcaaatgtg cagaagcttg gaagagcgat gctacttcca gttgcagcga
     1921 tgccattagc gggattgatc atgcgtttat cagctgatga tatgctgaac atcccagtaa
     1981 ttggtgcagc tggaaatgcc gtttttggaa acttggatat cttatttgct ataggagttg
     2041 caatcggttt tgtcaaaaat aaggacaaag gaatacctgc acttactggt tttttagcga
     2101 ttgctacctt gaaaaaaggc ttagaaatca tggacccaga agtgaatatg gggatttttg
     2161 gtggaattat ttccggttta gtagcggcat ggacgtataa tcgctttaaa gaacaaaaat
     2221 taccagtagt tttttcttat tttgcgggcg aaaaattccc attgactatg gtgatgattc
     2281 tacaaacaat cgcagctatt tttttgggct ttatctggcc gctcatccaa attgggattg
     2341 ataattttgc aaaactgctc gttgatatgg gggcattagg tgtggggata tttatgtttt
     2401 tgaatcgttt gctgatcccc tttggactgc accatgtgtt gaatacgtat atttattacg
     2461 atttaggaag ctatacatct cctagcaagg aagttttcag aggagagatg acacgcttta
     2521 tcaacggaga tcctcaggct ggactgtttt tatcaggttt ttttgtagta atgatgtttg
     2581 gtataccagc tatatgtcta gctatctata aagcagctta taaagaaaat aaagagatgg
     2641 tcaaaggaat catgggaagc ggtgcaggaa cttcatttat tgcaaatatc actgagcctg
     2701 ttgaatttag tttcatgttt ctttctccta tgttgtacgt gatccatgca ctttttgccg
     2761 gtttagcagg gattgtctgt tacttattca atatacgaat tggttttaca ttcggagcat
     2821 gtattgttga ttatctcatc aattttcgaa tcgctaccaa tgctattttg attcttccaa
     2881 tcggaatttt cttttttgcc ttgtattacg ttacttttta ttatttgatc aataagagaa
     2941 acattcagac attaggacgt gaagctaagg cagagtttgg caatgaagta accttagaag
     3001 aaacagagtt agggttagca agtaaaaatt atgaatatat ggcaacaaaa atgctacaag
     3061 cgtttggagg aaaagccaat atcctggatg tctatagttg taatacacgc ctacgggtag
     3121 aggtagtgga tcccaccatg gtcgaggaac agcgaatcaa gcaattaggg ataagcggga
     3181 tcattaaacc aactgaaaaa aattatcaaa ttatcatcgg cttagaagtg acttatgtta
     3241 tggcagaatt caataaatta ttagaagaat agaaaggaaa agatgatgat gaaaaaacag
     3301 attatcacta tagctggagg cgggagtacg tatacaccag gaatcgttca agccattttg
     3361 aacaatgaaa aacggttgcc attatctgaa atacgtttat atgatattga tgaagaaaga
     3421 aatatggata tgtacttgat tgttaaattt atgctgaaaa aaaaggtttt tcaaatattc
     3481 gcattcgtgc aacggatgat ccaaagcttg cttttacagg atgcgatttt gttttctcac
     3541 aaattcgtgt tggaggattg gagatgcgag aaaaggacga aaagattcca cttaaacatg
     3601 gattagtagg acaggaaacg tgtggactcg gaggatttgc ttatgggatg cgctcaatca
     3661 aaggtttatt ggaaattgtc gatcatatac aagactatgc ccccaaagca tggattttga
     3721 attatacaaa tccagagtcg atcgtttctg aagctgttcg tcgtaaatat ccgaatgtaa
     3781 aaatgattaa tgcctgtgat atgactattt caattgaaga aacgattgca gtgaattacg
     3841 gttatgatcg taagaattgg atcgttacgt attatggttt aaaccacttt ggctggtata
     3901 catcgattta tgataaagaa ttgcaacgag aaattatgcc agagattatt gaaaaactaa
     3961 taacaaaaga aatgcaagtc gccgatttta atataggaga caaaacttgg caaaaaacct
     4021 ttcaaatgat gtcagtcatc acgaaaaatt ttccaagtaa tatcccgaat aattacttag
     4081 agtattactt atatcctgat atggtcgttg agcatactga caaagagtat acacgtgcta
     4141 atatggtcat ggatggacga gagaaaaata ccaaagaaat ggctgataaa atacgccgcg
     4201 gtattgtgga agaagtattg aactttaatt ttggggagca tggacaatat atcgtcgata
     4261 tagctatttc cttgttgaat gatgatcgtc gtcgctttat gctaatcgtt cctaatcaag
     4321 gggcgattcc gaatctacgg tcagatgcag ttgttgaaat accggcatat gttggtgcaa
     4381 ctggtgtgga gacaattaca ctaagaaaac caatcagtga ttttcataaa ggattgatgg
     4441 aggcacaagt tgcagcagag aagttactag tagatgctta ctttgaaggt tcttatcaaa
     4501 aagctttaca agcatttaca ttgaatcaaa ccgtaccaaa tgccagagtc gcaaaaaaaa
     4561 tactagatga aatgatcaaa gtaaacaaaa aatattggcc agcattaaag tagaggtgat
     4621 aaaaagtgca tttctttaaa acaagaaaca ttctaaaagc tgtagctagt ggtcaaatta
     4681 ttccattaac agcagtaaaa gacgaggtct tttcttcaaa aatgatagga gttggatacg
     4741 ctataacgaa tcatgatgga tatatccatg cgccagttaa aggaataatc gataatatat
     4801 ttcctacccg acatgccata acgatcgaaa gtgaatctgg cagaaaaata ctgatccata
     4861 tgggtttaga tacagttgaa ctcgaaggaa gtccgtttca gatacgggtt gaaagtaacc
     4921 aggaggtaaa agaagggaca ctgttagcta ttatagattt gaagaagttg gagcaagcac
     4981 aaaaagatac ggtgatcatt gtagtttctc caggcatttt tgaaggaaat ttattaaaac
     5041 aaaaccaaga agtaaagatt aaagatagtt gttttgaatt ttagtgtacg atgaaaaaag
     5101 ttaaataggg tgatatttat gggtgaattt acaaaatttt tttgaaatg
//
LOCUS       DAGCYL010000171         5101 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.174, whole genome shotgun sequence.
ACCESSION   DAGCYL010000171 DAGCYL010000000
VERSION     DAGCYL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.174"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            44..1033
                     /locus_tag="MB216_002402"
     CDS             44..1033
                     /locus_tag="MB216_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4527188.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1015..1476
                     /locus_tag="MB216_002403"
     CDS             1015..1476
                     /locus_tag="MB216_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBT4527189.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1526..1990
                     /locus_tag="MB216_002404"
     CDS             1526..1990
                     /locus_tag="MB216_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4527190.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2004..2753
                     /locus_tag="MB216_002405"
     CDS             2004..2753
                     /locus_tag="MB216_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4527191.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2753..3583
                     /locus_tag="MB216_002406"
     CDS             2753..3583
                     /locus_tag="MB216_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBT4527192.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3583..4230
                     /locus_tag="MB216_002407"
     CDS             3583..4230
                     /locus_tag="MB216_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4527193.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4328..>5101)
                     /locus_tag="MB216_002408"
     CDS             complement(4328..>5101)
                     /locus_tag="MB216_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4527194.1"
                     /translation="NFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGVS
                     KITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTVL
                     HSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGSQ
                     YTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQP
                     FHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
BASE COUNT         1725 a          694 c          933 g         1749 t
ORIGIN      
        1 aataggtatc gtaaagttat ataactttat aatacctatt agagtgaggg ataaagaaat
       61 gagacaacta cacaaagaac agattgaaac agcaatggca gcgttaaagg aaaagacaat
      121 tgatcatgta tattttacag catgtggggg ttcacaagcc gttttaatgg ctggtcaata
      181 tctatttgat aaagaattaa ctgtaccttg tcatgtttat actgctaacg aatttaacta
      241 tgctatacca aaaggattat ctgaaaattc tttagttatc acttgttccc attctggcac
      301 aacaccagaa actgtagaag caacaaaaaa agcaagagaa tttggagcaa tgactatttg
      361 tttatctaat gaagaaggat ctcctctatg ggaagcagct gaatatcctg ttcactatga
      421 ttggggtaaa gatgttgatg ctagtgattt aaataaagga attctttatg gtttattatt
      481 taatatttta cttgttttag atggctctaa aaaatgggaa acttgcttga atgaatttga
      541 taagttaaat gatcttgtta ttgctgaaaa gagcaaattt gaaataactg ctaaagaatg
      601 gggtaaaaac caaaaacgag ataaaattat ttacacaatt ggtagcggta ttaattacgg
      661 agaaatttac tccacagcag cttgctggtt catggaaatg caatggatta attctaatgc
      721 tattcattct ggggaatttt tccatggtcc ttttgaaatc actgattatg atgtaccatt
      781 catcttagtg aaaagtattg gagatacaag atttttagat caacgggtag aagacttcac
      841 taaaaaattc agtaataaat taatggtttt agaccaagct gattttgact tagaatctat
      901 agatagtgta gcgaaagaat atattgcagc tgtacttagt ggcgttgtta ttcgccaaat
      961 ggtagaagcg attgcttttg aaagaggcca ttccttagat gttagacgct atatgtggca
     1021 aatgaaatat taattaatag gaggttgcaa aagatggcaa atcaattatt tcacattgtg
     1081 attacaacac attctaccct ttgtgagggt tatttaaagg caacaaaatt aattttaatg
     1141 aatgacgcca caggtattag tgctgttcca tttaaagaaa atactagtcc agatgatttt
     1201 gaggagagta tgaaagaaat aattagtcag catcatttgc aacctctaat tattttaacg
     1261 gatttaattg gaggaacacc taataatgtt tctattaaac agttgaacaa caatagaatt
     1321 caaattattg ctgggattaa tttacctctt ttactggagt tattaatggc ccagtcagct
     1381 ggtaaatcta tagaagaaat agattttaag gaattactga ataactctca aaaaagtatg
     1441 gttcatatta attcgctttt aaaagatgtc agttaaggtg gcaatttcta ttctataaat
     1501 tttaaaaatt gaaggaggaa atagaatgat taaattagtg cgtgtagacc acagattact
     1561 tcatggacaa gtaatatttt cttggactaa acagtatgat attaaccata ttattgtggc
     1621 tgataacaaa gttccaggag atccaatgtc tgtgatggct ttgacaattg ctaaacctgc
     1681 tgattgcaaa ttagacattg ttaaaattag tgatgttaaa gggattatgg aaacatcccc
     1741 aaatgataat tacatgattt taattaaagg acctaaggaa gcttttgaat tagttcaaaa
     1801 tttaccagaa attaaagaaa ttaattttgg cggtgtgtct aagaaaagcg agtccaaaca
     1861 atatggaaag gcagtctttt taactcctga agaattagaa gccacaaaaa aaattcttga
     1921 tcaagaagtg aaaatatttg tgcaacaagt cccatcttca agtattgaaa atgtagattt
     1981 tagtaagtaa ggggggaaaa taaatgttat taaaagccat tttattaggt ttagttggtg
     2041 tggtagctgt tattgactcc aggttaattg gtcgacaaaa tattggtaga ccactaattt
     2101 tgagtacact tgccggactt gtgctaggtg acttgcatac agggattgtt cttggggcat
     2161 ctttagaatt aatttctatg ggattcgtct caattggggc ggctggacct cctaatatgc
     2221 aacttggtag tattattgcc actgcttttg caattttaac taatagtagt acagaaacag
     2281 ccttaacaat agctatccca gtggctgtag ctggtgaatt tatgagtatt atcatgcgta
     2341 tgataattgc tcaatttgga catgctgcag ataaagccat tgaaaataat aaatttagaa
     2401 aagctcaaat tattcatatt tattggtcat ttatttttaa tgcttttgtt tactttattc
     2461 caatcttttt aacagtatat tttggtgctg acgttgtggc agatttagta gataaaattc
     2521 cagaaatgat tacagacgcc ttgactgtct ctggaaatat gctttccgca ttagggtttg
     2581 caatgctatt aagcacaatg ttgagtaaaa aattatatcc ttactttatt tttggattct
     2641 ttattgttgc ctattcaggt ctttcactaa ttggcgtgac aatttttgca gctctgattg
     2701 cctttgtaat ggatcaagtt aaatatggaa aaagagaaga ggcgcatggt taatggaaaa
     2761 tagtacagaa aaaaaacgtt taatcacaaa aaaagatctt cataagatgt gtcttcgttc
     2821 atggacaatc atgtgttcgt ggaattatga aagacaaatg catatgggat ttatgtatgg
     2881 gatggcgcca attttaaata aagtttataa aaatgatgaa gagaaaaaaa gagaagctta
     2941 tactcgtcat atggcatttt ttaactgtac gcctcaaatg actccattta ttatgggact
     3001 ttgtgcttct atggaagaac aaaatgctca agcagaagaa ggaaacttta atactgaatc
     3061 tatctccatg attaaaacca gtttaatggg tccttttgct ggaattggtg attcattttt
     3121 ccaaggtact ttaagaatta ttacatttgg gattggattg tcttttgcta agcaaggtag
     3181 tattttagga ccgattttag ctgttggcct ttttgctata ccttctattt tagctgtttt
     3241 ttacggcggt aaattaggat atgagtctgg caataaatac cttactaaat tgtatcaaga
     3301 aggtcttatg gatcgagtaa tgcaatttgc ttctatcatt ggtttggctg tagtaggtgg
     3361 aatggtggct agcatggtgc caattgtcac ccctttaaaa ttcactacag ggggaacaga
     3421 acttgtttta caagacttac ttgattccat tattcctgga ttattaccac ttgtatgtac
     3481 tttgggaata tataaattga ttaagaaaaa tgttagtaca aatatgctct tattaggttg
     3541 tattttggca gggtttgttt taagtataat aggtatctta taatgattaa ggctctaatt
     3601 tttgatatgg acggagtttt aattgatagc gagattatgt atcaaaagtg ggtatttaat
     3661 tttttaaaac aagaaaattt aagctatcct ttgtattctt atcaaaaaaa gattggaact
     3721 acagcaagta tttttgatga tatagaaaag tacaatgagg gatgtaacag tttagaattg
     3781 aagggaaaat ttactaaata ttggaaatcg caaaagattg attacttgga tatttttcct
     3841 aatatactaa ttgaacagct tgaatggtta aaaaagaaaa aaataaaaat ggcaattgct
     3901 tcttccagtc caatggatac tataaatgaa gttgttacca cttgtaaaat agagtcatat
     3961 tttgattgtt taattagcgg aagagatttg ccagaaagta aacctaatcc tactatattc
     4021 cttaaagctt cagaacagct cagaattcca gttgaagaat gtctggtgat agaagattct
     4081 tataatggta taaaagctgg taaaagagca aatatgaagg ttcttgcaat taaagataaa
     4141 cgtttttctc aagatgtaag tctagcagat gatgttattt acgatattaa atatctgcga
     4201 caagaagtac aggtgaaatt tggaatataa ataagttttt tgatgtattt agagaatatt
     4261 agtaaaaata aaaatcatgc ccaaatctaa tcaaaattga ttgctttggg tatgatttct
     4321 tttaatatca atctattact ttaggatctg aatcattttt atgcaccgta tctaaagtga
     4381 tattttctgt gacttcaata acataactaa ttctatcaca acgaactgtg gattgagaat
     4441 aatgaaatgg ttgattattt ttatctttgc caaccttttc taaaataaat agcggatctc
     4501 ctactataca ttgcaataag tcggaaagct caggtgttgc agataaaccg tttatttcca
     4561 taatagagct cgtagctttg acatcatagt catcttctag aagtttatat aatgaagtat
     4621 attggctacc tttttcaaat aaagttggaa atttttcttc tgatgcgaaa aaattatcaa
     4681 ttcccatcgg ggcaccgtct tcccacatta aacgtttaat attgtaaact ctatcagtta
     4741 cattgatttg taatttatta gctaaaaact catcagcctt tataatttta gaatgtaaaa
     4801 cagtatatct aattttatgt cctcgggtag aaagagaatc gctaaatcca tgaactccag
     4861 ctaattttaa actttctttt ttttgagtta caaaggtccc tttccccctt aatcgttcaa
     4921 ctgttccact ttctgttaaa agtttaaaag cttttcgaat ggtaatctta cttactccat
     4981 aaattttaca taattcttct tctgtaggta attgctctcc tggaagatat tttttttcac
     5041 gaatatcgtt acgtatagaa gcttctactt gtagatataa tggtctatta tcttgaaaat
     5101 t
//
LOCUS       DAGCYL010000172         5025 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.175, whole genome shotgun sequence.
ACCESSION   DAGCYL010000172 DAGCYL010000000
VERSION     DAGCYL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.175"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(98..1864)
                     /locus_tag="MB216_002409"
     CDS             complement(98..1864)
                     /locus_tag="MB216_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4527195.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1824..3578)
                     /locus_tag="MB216_002410"
     CDS             complement(1824..3578)
                     /locus_tag="MB216_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4527196.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3688..4257)
                     /locus_tag="MB216_002411"
     CDS             complement(3688..4257)
                     /locus_tag="MB216_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBT4527197.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4275..>5025)
                     /locus_tag="MB216_002412"
     CDS             complement(4275..>5025)
                     /locus_tag="MB216_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4527198.1"
                     /translation="TIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYE
                     NILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVM
                     QEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLV
                     SIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRI
                     IYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1670 a          945 c          676 g         1734 t
ORIGIN      
        1 gtattcaaaa ttagatgctg tttaataaaa ttattctttt tgaaaaagaa ttataactat
       61 atcccaaaaa ttgtaggtta atttaaagcc tatttcatca tcgtgattga gcttcttttt
      121 catagacgaa tttctcagat agtttagttt tctttatgag atttttgtat atttctgacc
      181 ttcgatacaa ctcactatga gtaccttgat caactatttt tccttgatcc atcactacaa
      241 ttttatttac cttttcaata gacttcaagc gatgcgatat aattagtact gtcttatctt
      301 gtattaaacg atttaaactt tcctgaatac gtttttcatt ttccgcatct aaactggcag
      361 taatttcatc taaaattaaa attggcgcat ttttcaaaaa agcccgtgct atggataggc
      421 gttgtcgctc tcctccagaa agtgaacttc cgttttctcc gaccaatgta tgataaccgt
      481 caggtagttt ttcaataaag tcttcgcagt tagctaaacg agctgctttt ttcacttcct
      541 catctgacgc ttgactattt ccaatacgta tattttccaa aatacttgta ttaaacaaag
      601 ttacctcttg aaaaacaata gcgattttgc taaacaaaga agctggagat acacgtttaa
      661 tatcataccc atcaattaaa atacagcctt catcataatc atacagtcta gacactaatt
      721 tcaagatact agttttcccg gaaccactag cacctactaa cgcagtcact tctccttgtt
      781 tagccgtaaa agagatatga tctaaaattg gggtattgtt atcatatgag aaagagacat
      841 ctcgcaactc aatgtcaaaa gatttcagag gactatctga tccctcttgg atactggttt
      901 ccttcatagc tctaattcgc tgtattttag gagctaaata aaatatttca agcactgctt
      961 ctttcataga atcgaaggaa tcctttattt ttattgcggc tagtaagtaa ccaacgacgt
     1021 agagtatagt cacttcaccc gttaacagta agtgaacacc tactagaatg actactgcta
     1081 aactaacata tgagaaaata gaggataagg ccatgactga aaaagtactt aattctacct
     1141 ttaaatgaat cctttcactc tcttccattt ttttgaaaag tctgtcttga acttttttag
     1201 atagattaaa gctattaatc tcttgctgca attctatagt ttcttgaaat tcttccgagt
     1261 tttctctcaa agtatcgtaa tatttagtat tggctttcgt ttgggatttc tttgataaca
     1321 agattaagac aaaactaaat aacgttggca aaataacagc taatcccatt ttgacattac
     1381 caaccaaaag catcactgaa ataaaaggga aaaacagagc cataccgccg gctttaggta
     1441 ttgcatgact catcgcatgc tcaatacctt caacgtcaga catgatagtt tgagataaat
     1501 ctgataaatt atgttttgaa aaataggata atggtaaatt tgacaaatcg tctgcaattt
     1561 gcattcttaa atgcgcactt tctttataag tactattata taagttttca tactccctat
     1621 ctaacaacca atacatgaaa agcaaagtaa cgaacgatac tgtaaaataa agccaaggcg
     1681 gctttacatt ttctaaaact aattcgtcta tcagcatcat tataatgaat gctggtacca
     1741 tattaatgca gtatacaaaa aagctagaaa tagatgcttt aaccaaatct tctgcgccct
     1801 gattagttaa agcgaatttg tttttataaa aacttttcat tgtcaagcct ccaatcattt
     1861 gcattctcat aattagtgac caattgttta tacacttgat gtttagccat taattcatta
     1921 tgatttccac gttctactat tttcccttct gacaagacaa gaatttcatc tacagattta
     1981 atgctggata atcgatgagc tatcataata actgtttttt ctttcatcag atgttgaaaa
     2041 gccttttgta gttcatactc attatctgga tcaatagatg cactggcttc atccataata
     2101 acaatctttg catcttttaa aattgctctg gcaatagcta tacgttgttt ttctcctcct
     2161 gacagataaa ctcctttact accaattaag gtatgctctc tttcgggaaa tttttctaaa
     2221 atcgattcgc aaccggctaa tcttagcgcg tcaaatactt tacttgctgg cgcttgagga
     2281 tttgctatag aaacgttatc gtaaatactc tgattgaata gtttcgtatc ttgaaagaca
     2341 aaagcaattg tgttaataat tgtgtctttt gaatagtgat ttaaaggctt accaccaatt
     2401 ttaatctgtc ccttatccac attgtagaaa cctgaaatta gtttagctat cgtactcttt
     2461 ccgcttcctg attttcctac taatgcataa attctttttt gttctaaaac taagttgaaa
     2521 ttttctaaaa cacactcttc tttgtatgaa aagctgacat tttcaaatgc aatgtcacaa
     2581 tttttaatct gtgtctcgtt cccatatttt agttggtctt gcttcatttc taaataaagg
     2641 gagtctagtg tttcgactgc atatcttgct tcaaacaagt gcatacttac atacattatt
     2701 ctcatgaaag aaacaaaaat aattccactt aaaaaaaaca acatcaacaa gtcaacagct
     2761 aatatcttgg gagtggtaag agaagttata aaaaaggtta taggaactat caaaatgcaa
     2821 actatgccaa aaaatagcca ttgaaaaaga acaaaaggtg ttttcccact caaagaatac
     2881 ttatatgcaa acttagcata atcgttaatt gctttattca aggctttaaa tgatgttact
     2941 ttagcaccaa atatctttat aacttgaatt cctcggatat attctaccgt ttcagagctt
     3001 aacacatcta acgattcttg atatttttga ataaaatttg tattctctgt cattttcttt
     3061 aacaagaata caccaattac taacatggcg attagaacca atccaatttt aaaattaatg
     3121 aaaaagccta aggcaactga taaaacaggt gttactatgg cttgagcact atctggaatc
     3181 aagtgagcaa ttgccatatg tgttttagcc gcgttgtcat caattgtctt tcgaatatat
     3241 cccgaagaat ttaaatcaaa aaaacgaaaa cttgcttctg ttaaaccgct aattcccttt
     3301 tttctcaaat tagtctctaa gcgaaaccca aggatatgag aaaataagcc agaaaaaata
     3361 taaaataaag ctcctaaagt taaccaagca acagtttgaa ccgcataact cttagctaaa
     3421 tattcatctc ctacaataat tagagactta agaaatttgt aaatactgta atatccatag
     3481 actgttaata cgacagaaaa atcagaaaat aaagtggcta accatccgta gtatttcatt
     3541 ttagggacat aactaaataa ttttttgtat actttcattt tttcctcccc cgcattcaat
     3601 ttcttctctt attcgtttac aactgttaat gctttccgta atagcgtaaa ttagagatgt
     3661 acaaacagat agaaggtaaa ctacgttcta ttttttgtat ttttttaaga cttttcttcc
     3721 aatcaataca ccaatcatag ataaacttgc tacaactaac actgaaatga ttccccatat
     3781 acctaccgca tagtggatca ttgcatccat tgttgcagca gattgtcctt tatctatcca
     3841 atcttgacga aaacgatctg taaagaacca tataggaatc cactgtccgg cagcactgcc
     3901 taattgcatt agagcccaag acgcaacttg aggaattgct cgatcatatc caaatacaga
     3961 tagaataata tcagctaaga tcgccatact aatcgaactg ataaaccaag gtaagtacgc
     4021 cccgcccatt aacgtaaaaa ataacaataa aacaattgtg taaatagtaa aaattccttt
     4081 tctagggtag ttatagctaa tataaacaaa tatgattcca gataataatc caaaaatccc
     4141 tggggatatg ctgtgaccaa acgccccacc aatactacta accattattg aaaaaaagta
     4201 aattactagg gaaataactg tagctaaacc aataaaaata taatcttttg ttttcatata
     4261 tattctcctc aattctattt cgttatgtca ttaaaaatct gttctatcaa agttgaatct
     4321 tctactctaa gttttatatc tttttttatt ccaccatttt ttaaataaat aattctattg
     4381 catgttttat aaagaaattc cggatcatga gtggctataa ctactgcacc cttttctttt
     4441 aagtctttaa taaggtcagc aatcctttcc atattaatat aatccaaccc acttgtaggt
     4501 tcatcaaata gaaataaatt agtttttgac aatgaagcta tagaaaccaa taatctttgt
     4561 ttttctccac cggataaatc aaacggtaat tgatctttca ttttataaag ttccattttt
     4621 tttaaaattt tttcttgctg atctttacta actatttcat tccccagggc taactcgctc
     4681 ctcactgatt ctgtgaaaaa ttggtaatcc atttcttgca ttactaaaaa cggcgatgac
     4741 aaattttttt tattttcgat tatctttcct ttggtagggc gaataatctt catgagtatt
     4801 cttagtaagg ttgttttccc cacaccgttt ttccctacca aaccaataac atctccaact
     4861 ttgacatcaa aatttatgtc ttttagaata ttttcataag taatttcttc aacttttaat
     4921 acatctgcac ttaccttttc gatataggga acttcaatcg aactattaaa aatgtcaaat
     4981 ttccttaatg cgttattgtt ttcagttttt aacaactgaa tagtt
//
LOCUS       DAGCYL010000173         5015 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.176, whole genome shotgun sequence.
ACCESSION   DAGCYL010000173 DAGCYL010000000
VERSION     DAGCYL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.176"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(113..1261)
                     /gene="nagA"
                     /locus_tag="MB216_002413"
     CDS             complement(113..1261)
                     /gene="nagA"
                     /locus_tag="MB216_002413"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBT4527199.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1337..2173)
                     /locus_tag="MB216_002414"
     CDS             complement(1337..2173)
                     /locus_tag="MB216_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4527200.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2357..3574)
                     /locus_tag="MB216_002415"
     CDS             complement(2357..3574)
                     /locus_tag="MB216_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBT4527201.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3723..3902)
                     /locus_tag="MB216_002416"
     CDS             complement(3723..3902)
                     /locus_tag="MB216_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527202.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3957..4040
                     /locus_tag="MB216_002417"
     tRNA            3957..4040
                     /locus_tag="MB216_002417"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3991..3993,aa:Leu,seq:gag)
     gene            complement(4280..>5015)
                     /locus_tag="MB216_002418"
     CDS             complement(4280..>5015)
                     /locus_tag="MB216_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527203.1"
                     /translation="SLAELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISS
                     FGKPFFLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFT
                     YVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDN
                     KKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMS
                     VQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1544 a         1029 c          805 g         1637 t
ORIGIN      
        1 taggaaatta aagaaacttt tgaaacttat gtaaagtgat ttttactaga acacaaaaaa
       61 aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta acttaaatta tattatgcgt
      121 ggaaacgttc cacgccatct agataagttg cttccaatgt catatcaggg ttcaatacga
      181 taaagtctgc atcgcgtcct tgagcgattt ttccacatgt atcatcgatt ttacagctga
      241 ttgctggaat cagactagcc atcatgactg cttgttctgg tgttgctaat ccccagtcta
      301 caacattttt aattgcttct ttcaatttca atatacttcc agctaagttg ccagattcta
      361 gacgtgctgt tccttctttt actactactg gaaattcgcc taaattgtaa tttccatcag
      421 gcatcccacc ggccatcata cagtctgtaa ttaatgcgac atgatcatgt cctgcttttt
      481 ccattaggat tcccgcagca tttggatgta catgatggcc atcacaaatc aattcagaaa
      541 agacatgttc taaagataat aacgcaccca ccatacctgg ttcgcgatga ttcaatccac
      601 gcataccatt gaaagcatgg acaaatacac ttgcgcctgc ttcaacagct ttttgcgctt
      661 cttctagtgt agcattactg tgtcctagag aaacaactac tccttcttca gtcacttgtt
      721 tgacaaactc ttctacacct tgacgttccg gtgctaaagc aatttttttg attaaaccac
      781 ctgaagcttc ttgccattca tggaaagtat ccaaatcagg atccccgaaa taacttgggt
      841 tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc aaaatagatt cctttgattt
      901 tagctcctgg tgcttcctga tacattttgc cgattgtctc agctacatct tttaaacgtt
      961 ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac tccacaagat aacagacctt
     1021 cagacatcac tttgatgcct tctgcatcat tatccattac atcatgattc atatagccgt
     1081 gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc gctttggtca atgatgttga
     1141 cttctccctc aggtagttct ttggtgtatt caccaaaaag tccatcggta atttcaagat
     1201 agcctgcatg tttgacacct gacgctaaaa agaatttgtc agcatagata aaagttctca
     1261 ttctattctc tccctttgtt tttctacctc taaagtactc ttatttagag aagaagtcaa
     1321 gaatggtcta caccatttac aaattttttg aaatttgttt cacagcttgt gctaagcctt
     1381 catagaatgc tgctctttct ggttcttctt tacgcttgta tattaagctt ttaagcacaa
     1441 ataaatcatt gaggtaatag tagctattct tctcttgagc ccaatagatt ccttgattca
     1501 cctgttctaa cgcttcgtcg atttcatttt gatggaataa gaaatttcca taattataaa
     1561 aaatttgcga aaattcactt tttgatcgtt catcagctga ttgatgcagc agttggatgc
     1621 ttcgactcaa atagtagcgt gcttcagccg tttttcctgc cacaccatat atttttccga
     1681 tacaactaat cagttgtatt tctgtgtcag aaacatggat tctttctttt tggctagagt
     1741 agcttaatgc ttctttcaaa taataaaggg actgagagat attttgacgc aacgtgaatt
     1801 cgcaaacacc gagataataa taatagcgtt gaaagtcagt tgcttgatac agattttcag
     1861 aaatatcaga agattttagt acttgttcta acttccgata atcacgtttt tggaaacaac
     1921 tatccaacag ttcaaataat ttatcgtttg tccgagtagt ttttcgagag agattcatca
     1981 catgatcaat cgttacccct agccgttgac aaatctgcat catcaccaat acattcggca
     2041 gttcctcatt attttcgatc cgacttaata cactttgtga acaaatatca tgcgaaagca
     2101 ttttttgcgt cattttttgt tcttttcgaa tctctttaat gatcgttcca aaggactcga
     2161 ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt cgcattctat tcgtatttta
     2221 ctttaggaag tcgcaaactt caagaaaaga cttgcaacta aataatatac tttagttatt
     2281 taagaaaact ccactagaaa gagagcctca gtgtgtttgt ctgtggccct cctactgaaa
     2341 aatttggttt ttaaaatcat ttttggcgat aggttgctag gaattctctc attttcttag
     2401 ctgtttgttt caaatcatcc attttaggaa gataaacgat tcgaaaatga tctggctggt
     2461 tccaattaaa tccaccacca tgtacaagta gaatatgatg ttcatgtaaa aagtccaaaa
     2521 cgaatttttc atcatccaaa atattgaatc gctttgtatc aatttttggg aatatataaa
     2581 aagcagcttt tggttttacg gctgaaagac caggaatatc attgattgca ttatagatat
     2641 attctctttg ttcatagatc cttccgcctg gcaggagcag ctcatcgaca ctttgataac
     2701 cgcctagcgc ggtttggata atttgctgag acaatacatt ggaacacaaa cgcatggagg
     2761 acaacatatt cagcccttct atatagcctt tgacgtgtga cttatctcca cttaggacca
     2821 tccagcctac tcgaaatcct gctacacgat gagatttgga tagaccgttc agcgtaacaa
     2881 cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc tagaccatcc atcactaagc
     2941 gatcatagat ttcgtcggaa taaataatca ggttattttg tcgagctact tcaacgattt
     3001 cttctaataa ttcttttgga taaagtgccc cagtaggatt attaggatta atgataacaa
     3061 tagcttttgt ccgactcgtt atttttgatt tgatatcatc aatatcagga taccattctg
     3121 cctcttcatc gcaaatataa tgaacaggtg tgcctccagc taatgcaata gaagccgtcc
     3181 acaacggata gtccggcatt ggaaccagta cttcatctcc attgtccagc aacccctgca
     3241 tacacatcgt gatcaattcg ctcactccgt tgccggtgta gatatcattg atcgtcacat
     3301 tcggaaaatg tttcagttgg caatattgct caattgcttt tctagcagaa aagatcccct
     3361 tggaatctga gtagccttct gagtcacggg cattcatgat caaatcacga atgacttcgt
     3421 ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt caattttaaa atactaatgc
     3481 cctcttcttg catccgctcc gcttcttcta gaacgggacc gcgaacatcg taactgacac
     3541 cttctaattt gttggatttt tcaaattctc tcatcactta gccaatccct catttcaaaa
     3601 agttagaact ttccaaattt tttgttattt actatttcaa tatcatactc catcctagaa
     3661 aattttgcca cttatttagg aaaaacgtaa gtattaaaaa aacacaaaca agcgttgaga
     3721 acttaattgt catgatagtc attccataga ttcgctgccc aatccattgc aattccacaa
     3781 atgattccta gcataaaacc aagcaaaaaa cgtccgaatg caagtccaat caatacacct
     3841 aagatcattc ccagtcctga aaattctccg tcatatttct ccatcttaat aagtcctccc
     3901 atttttgagc ttgacaacta tgtaaaacct cgctatcatt agtgtgttat tattacgcag
     3961 atgtgttgga attggcagac aagcatgatt gagggtcatg tgagcgttag ctcgtgtggg
     4021 ttcgagtccc atcatctgca ctacaactcg tgaagttaat tttgcttcac gagttttttt
     4081 gtttgaacgt ttctttatgc cattatatac atatcactgt atcctttaca aaaagaacgt
     4141 acaatcatgt aaacagcctg aggtgagtat atgaaggtcg gaaacaagct actcgtagaa
     4201 acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa atccctcacc taatactaaa
     4261 gcgagggatt ttttgtatat tatctgttta tcaccgttat tataaaggta caaataccaa
     4321 gcacggttaa tccaaattgc ataaattcat ttttttgtac actcatgctt ttcattagct
     4381 ctttaactgc cattagtact aagatggcga taacaacata aagtatatag tacgtccgca
     4441 ttccggaagc ccatactgtg ggagaaaatc ccataattaa ccttgaacaa aatcccatta
     4501 tcatcaaaat aactaaaaaa atagaagttt tcttattgtc aaaacttaaa taaattccaa
     4561 caattaaaca tagtaataat gccaagacta ctaaagtagc tatccaagta cctggataaa
     4621 aaagtgacag tttcgttcct aactctgtaa agagattatt caggttacta ctactccaaa
     4681 tcattgccct tttatttcca ttgacgtaag taaagctttg acccattgta ttaccaaaat
     4741 aaataatcag gtttaagaaa aacggtaatg cagtcaatat acgcacataa tagttttgac
     4801 atttcctata tgttagaagg aaaattaaga aaaatagcaa taaaaataag atattcatgt
     4861 ctaaaaaaaa cggctttcca aatgatgaaa tacctaaatc taacttatta agaaagttga
     4921 aatttttata ttcaggaaac catttattaa tttcatgtgc agcacgtaat gcattccctg
     4981 gagttgtcaa agagaaaatt aattctgcta agctt
//
LOCUS       DAGCYL010000174         4950 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.177, whole genome shotgun sequence.
ACCESSION   DAGCYL010000174 DAGCYL010000000
VERSION     DAGCYL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.177"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(92..1141)
                     /gene="recA"
                     /locus_tag="MB216_002419"
     CDS             complement(92..1141)
                     /gene="recA"
                     /locus_tag="MB216_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBT4527204.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1250..2491)
                     /locus_tag="MB216_002420"
     CDS             complement(1250..2491)
                     /locus_tag="MB216_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBT4527205.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2731..3309)
                     /gene="pgsA"
                     /locus_tag="MB216_002421"
     CDS             complement(2731..3309)
                     /gene="pgsA"
                     /locus_tag="MB216_002421"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBT4527206.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3518..4441)
                     /locus_tag="MB216_002422"
     CDS             complement(3518..4441)
                     /locus_tag="MB216_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4527207.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         1337 a         1124 c          894 g         1595 t
ORIGIN      
        1 aataactcac gatttcttta ttcaataaaa aaacggcagg tcaaggagag tacctactct
       61 tcagaccagc cgtttttgtt tatcataatt tttattcttc tactaaaggc aattcttctt
      121 gtcctttttc atcttctggg acagcgactt cttcaccaat accataagct gctctaacta
      181 gagcagaaac ttctgccatc atttcaggat gattagccat atagatttta gcgttttccc
      241 gtccttgacc aatccgatct tctttataag aataccaagc accgctttta tcaacaatgt
      301 ctttttctac tgccatgtct agaagttctc cttcttgtga gatccctaaa ccatacatga
      361 catctacttc agcgattttg aaaggaggtg ctactttgtt ttttacgact ttgatctttg
      421 tacggttacc aacaatatct gtaccttgtt tcaattgttc tgcacgacgt acttccaaac
      481 ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt gatttctgga ttcccaaaca
      541 tcacgcctac tttttcacgg atttggttga tgaaaatagc gattgttttt gttttgttga
      601 tcgaaccaga gagtttacgc aatgcttgag acatcaaacg tgcttgtaac ccgacatgtg
      661 agtcacccat ttcgccgtcg atttctgctc gtggaactaa agcagcaact gaatcgacta
      721 ctacgatatc tacagcccca cttgatacta aagcatcagc gatctctaga ccttgttctc
      781 ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac acctaatttt tgtgcatatt
      841 gcggatctaa cgcatgctca gcatcaatga aagcggccgt tccgccattt ttttgtactt
      901 ctgcaatagc gtgtagtgca actgttgttt tacctgaact ttcaggtcca tatacttcga
      961 tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac atctaacgca agagagccac
     1021 ttggaattgt agagatttgt tggtcgattt tttcgcccaa tttcataata gagcctttac
     1081 catagctctt ttcaattttt ttcagtgcag catctaaggc tgctttacga tcatctgcca
     1141 atacgataat cctccttata tttacaaatc agagcaacat ttcattatcc ttatcatatc
     1201 ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat tcgcatttat cattttctta
     1261 aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc attatatctg atagcagcac
     1321 gatctctggc aaagtgatat ttttccgaaa ccacgccgtc tttagatgaa atagcgatcc
     1381 atactgttcc tgcagattga ccttctaacg tgtccggtcc agccactccg gtgaaagcta
     1441 cactgtaatc tgttcttgct atctcacgtg cttgaatggc catctgctcc acacattctt
     1501 tgctgactgt tccatattct gctaatagct ccggatcaat ctccagaaaa ccagcttttg
     1561 tttggagcga gtaggtaacg aatccgcctg gaaatacttc agatacacca gcaatgtcgc
     1621 ctaatgcggc ttggaaggcg ccggctgtca aactttccgc tgctgttaca gtctgcttgt
     1681 tttctttcag tagttccact acgacttttg ctagcgaatt atcatcgccg tatccataaa
     1741 aatattcccc tactcgttct tggatttttt cttctaatgc taaaagagct tgattccctg
     1801 cctgaacatc atttgttttc actgtcagtc gtaatgtcac ttcatttggt ttggcatatg
     1861 gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt tagctcagtg accagtcgcg
     1921 attcaccaat cccataaaag cgcaaaactt tagaaattag tttttcttca gaaggaaaac
     1981 gttcctctag taaaggacgt acttgttcca caaacattgg tttcaactca ttaggcggac
     2041 cgggaagaag gatatacgca tgtgtatctg tttgataaaa agtccctaaa gcaagccctg
     2101 tacgatttgg tagaggaacc ccgccttcga taatctggga ctgttgcaga ttgtttttgg
     2161 tcatttttcg atgggttgtt tcaaaatatg ccaataattt tttgtatcct tccgtatttt
     2221 gtatgagaga cttaccgagg tgagccgcag ttgcttcttt tgtcaggtca tcttctgtcg
     2281 gtcctaatcc gccacataag agtatgagtt cacttcttgt ttcagctaga gcaatcaact
     2341 cttctaaacg ctgctgatta tctcctacaa ctgtttgata ataaacttcg atacctagat
     2401 cagctagctg ttctgacaag aaagtggcat tcgtattcac gacttgtcct agtaaaagtt
     2461 ctgttccgac agctattatt tctgctttca ttacttcccg ctcccttata tagtatacgc
     2521 tgtattttta tattttttta tttaaaaaga gggcaaggag ctgtgacaaa aatcttgtca
     2581 cagctccttg ttccaaataa acagtgttct cctgccaaaa cctcggtcat aagtcacaat
     2641 atacataaaa catgaaaagc tgttttttga aattatccct tgtgactcag agttagacgg
     2701 caggctcaca acctctccac taaacgatac ttacattgat cctttgaaca caccgctgtt
     2761 tttgatgaag taatccacac ctgaatagat agtaaagatc agacaagcat aaagcatgat
     2821 ttgatctact ggaataccga tcaggctgaa tgggatatta ttgataaaca gcaagataat
     2881 agcaaccatt tgtgtagccg tcttgacttt tcccggccaa gctgcagcca tgacttctcc
     2941 atgttcgaca agtagtaaac gcaagcctgt gacggctaat tcacgacaga caatgatcgc
     3001 tactacccaa ccaggggcct ttccttggtc aaccaacatg ataaaagcag tcataactag
     3061 cattttatct gccaacgggt ccgcaaactt gccaaaatta gtgacaaggc ctcgcgcacg
     3121 ggcaattttt ccatctaacc agtctgtgat actagctaaa gcaaaaataa ctgcacctac
     3181 tagctgtgtg acagccagtg tggtgtctcc tacgttaaat tgtccccaat ctaaaggtaa
     3241 acctacaacc aaaatgaaaa tcgggatcat acaaattcta agtacagtta atttatttgg
     3301 taaattcaca ctatcgactc ctttcttccc tattctacgt gactatcagc gaagtttcca
     3361 ccgatattat ttacttttcc aaatgaattt attcaaagga gtatttttat tgaaattgat
     3421 ctttttgaat taacgaagaa ggaccgcgac acagatttgt cacgatcctt cttttttata
     3481 cctacaagta aacagatgcg taaactaatt tatacgatca ttctgcgtat tgaagagtaa
     3541 ggttcacatt ttttggactc tgattgtttt cccctggatt aactggtacg gattctccat
     3601 tcgctttgat cgttgcattg cttgctgcac caatcgtgat ggtcgcattt gttgctgttt
     3661 ccggtaacac ggtactctgt gtttcatttg ctgccagtgt cccttgataa acataggcat
     3721 tgtctactag cacacctaac catacacgat tgacagcagt aaattcaagg gttacaggct
     3781 tggtagcgtc tgtgatattt actgcgatgg cactgccaga atcttctcct gtagctattg
     3841 ccattttctt ttcttcactt gattttgttg ttgattctac tgtagaagac tctttggtct
     3901 gcgtgctttg ctctgaggaa gaggactcct cgactgatcc ttctacttta agcgaagaag
     3961 ctgtgtctcc gattattgga tcagcttgac gatcttgcca agtcatatag cccacgacaa
     4021 cgacaatcgc caaagcaacc aatcctaata aaatgacagg aagacttgtc caaaagcgac
     4081 ttggatgcga ttcttcctga tgcattgctt ttctagaagt acttactgtt tcaggctgcg
     4141 gacgttcagg gaccgatcct gtcaatttgc ttttcccatc aaaaacatcg actaaatggt
     4201 ctccatcttc tcctacagct tcagcgtatt gccgaataaa tgcccgcacg taaaatttac
     4261 caggcaattg gtcaaaatca tcattgtcga ttgcttccaa ataacgtttt tgaatttttg
     4321 taatctgctg taattcatct aaagatattt ttttattcag gcgtgcttgc cgtaattttt
     4381 ctccgatggt ttcaaaagcc actcgttcat cttctccagt ctgtttgatt ctccgaagca
     4441 tctgctgttg caacgaaaga cgttatgttt gggtgctgtg acaaaagtca tgtcacagct
     4501 ccttttttcc gaataaacat tgttctcctg ccaactcctc ggtcacaagt cacaatatcc
     4561 aataaatata agaagctgtt ttttgaactt gttccttgtg actcggagtt aaacggcagg
     4621 ctcacgacct ctttcttaaa cggtgttcaa tcagctgttc ctcggtatta gctcaaaaac
     4681 cggcaaaata taaggagata tttttccgta tgtttctcta atactcaggt caaaacgctg
     4741 atttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagtccaa
     4801 ccaagttaaa aacattaaga agctgttttt acttggctgt tcttattact tgatgcaggg
     4861 cagctttttc acaacctctt ggtacacggt gttccagaag tagcttcttc ggcaataagc
     4921 cgaaatatca caaaaatttg agaagcaatt
//
LOCUS       DAGCYL010000175         4908 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.178, whole genome shotgun sequence.
ACCESSION   DAGCYL010000175 DAGCYL010000000
VERSION     DAGCYL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.178"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(143..2047)
                     /locus_tag="MB216_002423"
     CDS             complement(143..2047)
                     /locus_tag="MB216_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBT4527208.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTDMAPESALVQTEDGEFEEVDVDDVDEGDVLLVKTGAKVPVDGTVL
                     TGEGHINEASITGESVPVTKTEGSEVFAGTILENGTIQIWADKVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKWYTPTVLVLAFIVWAMTQNVELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVINDFSKVDTIVFDKTGTLTVGNPEVAETEVYGDNVE
                     KAYSYLASVETESDHPLAKAVLQHTGTVELFPVEDTDVVKGGGVVAQVDGHRIAVGNV
                     ALMEKENVQLSKKAQKDVKQFEKNGNSLVLTAVDGELKILMGIRDQIRPGVKQDLQEL
                     KNLGVKDLVVLSGDNQGTVDRVARELNLTEAHGHMLPEDKAAYIEKLQQKGHTVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIY"
     gene            complement(2245..2478)
                     /locus_tag="MB216_002424"
     CDS             complement(2245..2478)
                     /locus_tag="MB216_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBT4527209.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGVDKDSIKVLFNSSKV
                     KANFDSAVTSIEEIQKAIENVGYPVLKAKVKAA"
     gene            2717..3757
                     /locus_tag="MB216_002425"
     CDS             2717..3757
                     /locus_tag="MB216_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HBT4527210.1"
                     /translation="METERQIRELEKAMNTIDNRRLYERFLAVKLVLEGHTRKETGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKAGSKKQQVFLE
                     ETFPSLKKKLLNSDIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIG
                     TLDYETGRIICTEEGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLE
                     ENARRLALIFLPPYRPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNER
                     PLEVIDRLCVRM"
     gene            complement(4072..4671)
                     /locus_tag="MB216_002426"
     CDS             complement(4072..4671)
                     /locus_tag="MB216_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4527211.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLSDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFLEQ
                     LQVQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTVVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
BASE COUNT         1326 a         1140 c          934 g         1508 t
ORIGIN      
        1 ttaattttat aaagaacata ctctatactc ttattttttt tcaagaactc aaaaaagcgg
       61 aaaatcccag atttaaaaat ctgagtttcc gcttttgtca tttcaaggat ggactattgt
      121 ctcagcctct tctaatttat tcttaatata tttttacttt ctctgcaggt gctgatactt
      181 ctttttgttc tttccgacct ttcaacttat acttcattaa tctcatgcca ttgaaaatta
      241 ccaccaagat actaccttca tgaaccaaca ttccgataga catattcatc cattcgctga
      301 agaagacgct ggtcaacaag accaacacta ctccaactga aatcacgata ttttgtttca
      361 tattatttgc ggtggctttt actaatccta atgcgtgggg caagttgctg aagttcgagt
      421 tcattaaaac gacatcggat gtttcaatcg ctacgtccgt tccgcttccc attgcaatcc
      481 cgatatctgc tagcgccaag gacgggctgt cattgacacc gtccccgaca aaggcgaccg
      541 tgtgcccttt ttgctggagt ttttcaatat aagccgcttt gtcttccggc agcatatgcc
      601 cgtgcgcctc ggtgaggttc agttcacgcg caacccggtc cactgtgcct tggttgtcgc
      661 cggaaagaac cactaaatct ttcacgccta aattcttcaa ttcctgcaag tcttgtttca
      721 ctcctggacg gatttggtcg cggattccca tcagaatctt caattccccg tcgactgctg
      781 tcaatactaa ggagttccca tttttctcga attgtttgac gtctttctgc gcttttttac
      841 tcaactgcac attttctttt tccatcaacg ccacatttcc aaccgcgatg cggtgtccgt
      901 ccacttgagc gacaacgccg ccgcctttga cgacgtctgt gtcttctacc gggaacagtt
      961 ctaccgtgcc ggtatgctgg aggacggcct tggctagtgg atggtctgat tcagtctcca
     1021 cgctggctaa ataactgtag gctttttcta cattgtctcc gtacacttct gtttccgcga
     1081 cttcaggatt cccgacagtc agcgttcctg ttttgtcgaa cacaatcgta tcaactttgc
     1141 tgaagtcatt gatgacttcg cttcctttca aaagaactcc attgcgtgca ccgtttccga
     1201 ttccggctac gttggagacc ggaaccccaa tgaccaatgc acctggacag cctaggacta
     1261 agatcgtaat cgccaattca acgttttggg tcattgccca gacaatgaat gccagaacca
     1321 aaacagttgg ggtataccat ttagaaaagc ggtcaataaa gcgctctgct tctgatttag
     1381 aatcttgcgc ttcttccaca agttcaatga ttttaccgaa tgtggtgtct tctccgactt
     1441 tgtctgccca aatctgaatg gtgccatttt ccaatatcgt accggcaaag acttcactgc
     1501 cttctgtttt tgtgacagga acggattctc ctgtgatact ggcttcgttg atatgccctt
     1561 cacccgtaag gactgtacca tctactggaa ctttcgcacc agttttgaca agcagaacat
     1621 ctccttcatc tacatcatct acatccactt cttcaaattc tccgtcttct gtttggacca
     1681 aggcactttc tggtgccatg tcggttaatt ctttgattgc tgaacgtgtc tggttcaatg
     1741 tacgctgttc caaataatgt ccgaacaaga acaagaacgt aacgatagcc gattcttcat
     1801 aattttgaat aaaaagcgca ccaatcgctg caatactgac taagacatca atactgatga
     1861 ctttgacttt cattgcttga aacgcttgaa tggcaattgg cgtaatccca aagacagagg
     1921 cgatgatcaa agaccactct gaaagtccta cgttttctaa aacaaagtga ctgaagaaac
     1981 cgagtacgat taacaatcca ctgatgacgg tgataacatt tttcttgctt aatatatatt
     2041 gttgcatgct ttttccctcc aaatttaatt tctcttttat tgataaacac agtataagat
     2101 aattgcctaa gtaattaatt gactgacatc aagtttagaa agttctcttt tatcgtttct
     2161 aagcacctcg aatcggattg caacaaaaaa agcaatcctg gaaaaatagc ttttcttcca
     2221 gaattgccta gttcatttaa tctgttaagc ggccttgact tttgctttaa ggaccggata
     2281 gcctacgttt tcgatagctt tttgaatctc ttcaatcgaa gtgacagctg aatcaaaatt
     2341 ggctttgact ttgctggagt tgaataatac tttaatgctg tctttatcaa caccgttaac
     2401 tgattttacg gcaccttcaa ttttttgcat acaacttgga caagacaacg tttctaattg
     2461 caatatagct ttttccataa ttcctatctc ctttatgtgt ttttatttta tcggcagcag
     2521 attaatgaac actgcctttt cctttttaca actgtagtat agctcacttt tagcaaacga
     2581 aaattgacac atattaagtt ttgcacttct ttttatcttt ttgctcaatt caccatctta
     2641 tacaaattga gttaaattgt attcgttttt cctcctatag gcgtatacta aagaaaacac
     2701 ttgaggagtg gggaacatgg aaacagaacg ccaaatccgt gaattggaaa aagcgatgaa
     2761 tacgatagac aaccgccggt tgtacgagcg tttcctggca gtcaagttgg ttcttgaagg
     2821 ccacacccgg aaagaaaccg ggcacaccat cggccgcgac gagcatactg tcagccacta
     2881 tgtgaaaaac tactgccata atggaatcca aggtttagtc tccaaaaaac aaagcggacg
     2941 accttcacgg ctgacagatg aacaggaagc ggaactggtc caaatcgtcg cctaccacac
     3001 tccggaggaa gtaggtttca aatcccgtgc caattggaca ttggcgattg tgtgtgacct
     3061 gattctccgg gaatggagtt atggatatac gcaaaaaggc gtggcggacc tccttcaccg
     3121 gctaggacta agctggaccc gtccgacgta taccctcaaa aaagcgggtt ccaagaaaca
     3181 acaggtattt ttggaagaaa ccttcccgag cctaaaaaaa aaattgctga atagcgatat
     3241 tgaccacctc ctattcgagg acgaaaccat gatccgggat taccaagcca ttggctgcac
     3301 ctggtttctc cgcggccacc agcgaaaaat ccccacttat ggacaacaca aaggggtaaa
     3361 attgataggc accttggatt atgaaaccgg tcggatcatt tgtacagaag aaggggcata
     3421 cgacgcagcg gccttcctcc gttttctgga gcggacgttg cgggaatacc ccaccggaaa
     3481 aatcgtgatg gtattggata atgcccttat ccaccacgcc aagctgatcc agtcttttct
     3541 tgaagaaaac gctaggcgcc tggcactcat cttcctccct ccctacagac ctcagttgaa
     3601 cctaatggaa ggcgtttgga agtggctcaa ggaatcggtc atcaacaatg ttttttttga
     3661 ccacgtccag aaaatcaaac agtcggtccg cggtttctta gccgatgtaa acgagcgtcc
     3721 ccttgaggtg attgaccgac tgtgcgtgcg gatgtaaagt atttaattca acctatatag
     3781 aaagaagtct tgtacttcat tttagtctaa tgacagtcat aacatcatcg acagccccgt
     3841 tattgagtca attctattca ataatttctt ctacttgtaa taacgttgta ttcaacgctt
     3901 aaaatattcc agcattaaac accagttcca cactgttctt tttcaataga tacaccgatt
     3961 ctttgcgaga taattggtat ttatcaggag atttaggacg gttttctacg ttcattttcc
     4021 cacctcttga atttttcctt ctattatata ggtatatagg gcgttattct gtcatagatc
     4081 caaactcttt actgcggtat ctttgtcttc ctgggtgatc ccaatatagc gtaaggtttc
     4141 ccgttgtgaa gaatggttta gtagtgactg aatgatctct gttttggttc cttgcttgta
     4201 caaaaaatag ccaaaggtct tacgcatcga atggggacca atttctttca acccaaccac
     4261 cgttcctgct tcatgaatga tccgccaaag gctttgtcgg gtcaaaggcg tatctgctcc
     4321 ttttcgactt ggaaacaaat acttgctgaa gtcttgtact tgcagctgct caagaaattc
     4381 tgattgaaca taatcaagaa tcacttttcg taatttgttc gaaatgaaaa ccgaattgtg
     4441 tttagctgtt ttttgttcct tcatggccag acgttctttg acgatcaccc ggccacggga
     4501 aatttctaac acgtcactta atttcaactg tcttaaatcg gagattcgat acgccgtatt
     4561 gatcccgatc gaaaaaagca ccaaatttcg ttgcccatat tttccggaag ccaaaattgc
     4621 cttcatggcg tcgatctttt ttttatcgcg aatcggttcc acgctcttca tttttagtcc
     4681 cctctcaaat gttacttaat atgattataa cagataataa gtcacattta aacaagctat
     4741 agaaaaaagg ccctaaaaga aaagtgattt cctcttagag cctctaagga tggagcgatt
     4801 ttgatgaatg tgacagaatc cgattgtgtc acattaggct tttgatattt tcggtaagat
     4861 ttgattatct cagaacatta atattgcccc tttaaaaaat tttgtcag
//
LOCUS       DAGCYL010000176         4861 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.179, whole genome shotgun sequence.
ACCESSION   DAGCYL010000176 DAGCYL010000000
VERSION     DAGCYL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.179"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1219)
                     /locus_tag="MB216_002427"
     CDS             complement(<1..1219)
                     /locus_tag="MB216_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBT4527212.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSFFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEM"
     gene            1280..1855
                     /locus_tag="MB216_002428"
                     /pseudo
     CDS             1280..1855
                     /locus_tag="MB216_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2036..3229)
                     /locus_tag="MB216_002429"
     CDS             complement(2036..3229)
                     /locus_tag="MB216_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4527213.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(3313..3513)
                     /locus_tag="MB216_002430"
     CDS             complement(3313..3513)
                     /locus_tag="MB216_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBT4527214.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3937..4170)
                     /locus_tag="MB216_002431"
     CDS             complement(3937..4170)
                     /locus_tag="MB216_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4527215.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(4177..4608)
                     /locus_tag="MB216_002432"
     CDS             complement(4177..4608)
                     /locus_tag="MB216_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBT4527216.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1325 a         1110 c         1003 g         1423 t
ORIGIN      
        1 acatttctac aaaaaaatag tgattctgtt ttgaaaattc gatttttagt tctgcatttt
       61 cgtaaacatc caacattttt ttcgcactgt atagacctaa cccgccattt tttttagagg
      121 tttttcctct tttgaccatc tccgatagcg tcaaattcgt tggatttgcc ttgttcgcta
      181 tactaaaacg tgatccctct ttttcttcca gcagattgat cgtaatcgga ctctgatcgg
      241 ctacacgatg atcgaatgcg ttagaaataa aaatagataa acagcgaata aaaacaacca
      301 gatcaatcgg gattgttttc acttcgacag gaataactac ttggatcggg attttctccg
      361 attctgcttt ttctagaaat tttaaaaaga cacccctgac agctggggat acaatatttg
      421 ttagctgttg tatttgcggt ttttcaaaaa tagctgcaga atgatcttct aatatttcta
      481 aaaattccaa cgtttctttt ttattttcct gaagagtaat ttttaatgaa aacagtagac
      541 ttttataatc atgtttgaat tcctctagat gatggtaata ttcttcattt atttgataca
      601 gttgaagcga ttcctgatgt tgtctttctt ttacttggaa actgatcaac ataatggaaa
      661 aaaaaaaaga aaaaaaactg atgactatcg acaaaagaga tagaagcaga taattagatg
      721 agcggaagtg aacacttccc aataccaagt aaatcacgcc ccagcttaat aaaatacaaa
      781 tacctccaaa atagtgttca gttggataag taaaaagcaa gttgagaaat ctcgtctgtt
      841 tgtctatact ttttactaat agatagaaaa taaatgcgat aagtagtgca aaaaaacttg
      901 ctgttccaag atgtctcttt cctaaataat ccatcagatc aaaaaccaga aaaaagctga
      961 gtccagtgat agcaaaaaaa agattactga tcaaacagct gataaacagg tttttcgtag
     1021 taaaataaaa aagaaacatc aataataagc caactaggta aggaaagctc actttaggga
     1081 taggaagtaa aagtgtcacc accatcaaag ctatacaacc aaagctgttt tttactagca
     1141 gttgtttcga aaagaataaa tagataccta aatctaaaat caaagtaaag gtcaaataaa
     1201 gtgtcactgt tgcgaccatc ttattcctcc attgttttgt ttcaaaatgt acacatattt
     1261 ttttaatagg agcctgcata tgaaaaagta ttttatcata gaagataacc agtttcacag
     1321 cgaatggatc atcagagaat tggtcaatca tcatatcgag aatatcgtgc agctcacttc
     1381 tatctctcaa atcaatcagt tgatccagtt gggcactatt gataatcacg atgttttctt
     1441 tatcgatatc gacttgaagc aatcactcac aggtattgat gtcgccgaac aaatacgtaa
     1501 aaaaaatgca catagtaata ttgttttttt caccgcttat acgaattttg cagtagagat
     1561 catcaatcgg aaaatccttc cgattggata tatcagaaaa gacaggaatt tatctgaaca
     1621 aattcgtcaa gtgctagaac agatcaattg gaaagaacag cagatcgtaa atgtcgataa
     1681 aatctttatt gatcaaaaag gtggaagtct cgtcctgtat cctcgggaaa ttttatatat
     1741 tgaaagtatc aaaggtttga aaaaccaagt tatggtcaaa acaactacag aagataaatt
     1801 agtgaatact tctttaaaac aattgaagac agagttagaa aagtatgctt actttttctt
     1861 aaaaattata tgggatttta gattatcttt ttagaagttc ttgaatgtcg gtatagcccg
     1921 tgtttccggg cttttccggc atttttgata ttgttagata cttacaaatg ttcttattac
     1981 ctctcaccat ttccccctca aaagtagtaa atggagtagt aaaaccggcc ttgtgtcagg
     2041 ctgccagccg ttccatttct gcccttgcgg aatcgaatgt ggcgtgtgca taatagttca
     2101 gcgtcatggt gatattggaa tgtcccataa tgtactgcaa ggctttgggg ttcattcccg
     2161 cattggctaa ccgggtgcag aatgtatgac gcatagcatg gggcgtcgtc gttttcggca
     2221 acgcttcttc atggctcttg ttgtacttct tcacaagcct gccaaacatc ccgccataat
     2281 ctaccgccgt catgggatag ccgttctgtt tgagaaacag gaaattggca tacccgtcaa
     2341 taatgaacgg ctttgcacct ttgcggtttt ttatcactcg ctggaacgcc tgatacactt
     2401 cctcattcat ggggattttt ctgacgccgc tttgggtctt gggttcgtca atatagtaac
     2461 cgacgccggt atttctcaaa agctggtggc tcacgatgat tacccggttt tcaaaatcca
     2521 aatctttgtc cgtcagtccg cacagttccg aaatgcgaag ccctgtcccc agcagaataa
     2581 cgattgcgtc ataatgcttt tggtaaacct tatcaccttg aataaaggac agaaaacttt
     2641 cttcctgtgc tggtgtaaga ggcacctttg gttccgtatc gtcgtccaga acgtcactaa
     2701 gctggaagtc aaacgggttt tttcggatgc aatcgtcctg tatggcggtg taaaaagccg
     2761 ctttcaggga acgcttgtcg ttattgatgg tcttataaga aaggcccttt tctttcatcc
     2821 gaagcgccca ttcctttgcg tcggacagct tcacactgtc aatcgggcag ctccccagcc
     2881 gatcttgttc cagaatacgc atcagccgtt cccggcccag tttggtgttg tgcttcacat
     2941 tgccccggtg cctgatctgc ttggcgtaga gctggcagac ggtcattttc ttgccgatgg
     3001 tgtctatccc gtcgtcaagg tctttctgga tttctttctc cttttcccgg agtgaaatat
     3061 cctcccgttt acctgccggg gtcttatccg taggcactaa cttccatgag taaacaaact
     3121 gcggttttcc aaaggtatca acatatttgt aagcatatcg tccgtctttt ctctggctct
     3181 ctccagaccg caaaacgcga tttttgctgt ctcgtctttt ttctgacatc tcgtcatgct
     3241 cctttccatg acggaaagag ccgcatatat gcttataaat tattataaca tatctgcggc
     3301 cctttttcac tattagattg tgtccaaagt gtcaattatt ttctcaaact gcttgcgttt
     3361 gatctggatg cggttgccat tcataatgac ccagccagag gccggatttt cctcggccag
     3421 cttacgcaat ttgttttctc cgatacggaa atatttagac gcttcctcaa tcgtcagcgt
     3481 gtatttttcc caaataggca catcatggtt cataatgcag ccccctttca caaattcaaa
     3541 ggtttgtgtg atttaggggc atagacggac ggctgctggc acagctccac gggactttca
     3601 cccccgcgtg gttctcacgc ggccctaccc attgcctgcg acgcttttaa cgctcggact
     3661 gtggctataa gggagtatca tagtcctgcc tgtacgtcgt cgcccgcaag ccgccacgct
     3721 cacattgcgg cgtggtgttc ctcgctccgc ttttcccgta gggaaaagta tcatggcgca
     3781 ccagccgtcc ggctcggcac agacagaatg tgtccgtatg ccctatgatg cgccgccgtt
     3841 atcttgcggg catatttatc aaggtgcgat caggccaccg ggaaaacacc gaaacgggga
     3901 aagggtaagc cgtttcatgc tgccggggcc ggttcgtcag ttcgtgacga tagcgtggat
     3961 gagcttgatt tccagacggc gcttcatggc ctcgtccaga cacacatgcg gatagccgtt
     4021 ttcgtcatag agcgtccggg tacacagctt gttcatgtaa ccgctgtaat actgcaaaac
     4081 gcgctctacg gctccggcat ccccagcgcg ggcggcctcg atcaccgaaa ggggcaaaag
     4141 ctcaatgccc ttgtagttcg ggttcttcat ccgcgtctaa cctcccttcg gcatcaacgc
     4201 ctttagctgt ttccgcagct ccgataaggt atttgtcctg tgccgctgaa cggcactccg
     4261 ggacattccc acaaggccgc cgatctcacc atctgccata tccagaacac agtgcaggat
     4321 taaaatactc tgctccatgc tgggcagcgc ggcaaaggct tcggcaacaa gctcattgtt
     4381 gatatgtagg tcatagccat gcgacgaaaa cgtaaagctg tcgctggggt agtgatctac
     4441 tgtacagagc ttgtccaaat ctgcctgtga caaagagctt agtgacactt cacggtcacg
     4501 gcggcgcttc atttcagcca agtagctttt ggcctcgtta cgcagtaccg tcttgcaaaa
     4561 agcatcgaac atgcaccgcg cttcatagtc gcgggggata gcatccatct tctcaccccc
     4621 tttctgtggg ggacttaggt ggttctctcc ctttccgcta acaagacttt gacaaggccg
     4681 ccagctaccc gctaaatacc tcctttttga aaatttctca aaaaattttt ttggggacag
     4741 ccgggccgca aaagacgaca aaaatcgccc ggcaggtcgc aaacctaccg ggcgagaaaa
     4801 tgctttgcta tgctgctgat ttacatggta tagtacatac tcataaccgg aatagccctg
     4861 t
//
LOCUS       DAGCYL010000177         4858 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.180, whole genome shotgun sequence.
ACCESSION   DAGCYL010000177 DAGCYL010000000
VERSION     DAGCYL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.180"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            72..443
                     /locus_tag="MB216_002433"
     CDS             72..443
                     /locus_tag="MB216_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527217.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            528..728
                     /locus_tag="MB216_002434"
     CDS             528..728
                     /locus_tag="MB216_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527218.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            862..2088
                     /locus_tag="MB216_002435"
     CDS             862..2088
                     /locus_tag="MB216_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527219.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            2938..4262
                     /locus_tag="MB216_002436"
                     /pseudo
     CDS             2938..4262
                     /locus_tag="MB216_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            4255..4602
                     /locus_tag="MB216_002437"
     CDS             4255..4602
                     /locus_tag="MB216_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527220.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
BASE COUNT         1890 a          592 c          909 g         1467 t
ORIGIN      
        1 gatcttttat cacttatata aagagagaag tgataaatag gacacaaaat ttggaaagta
       61 ggtaagtcac tatgagagaa ctaactttat cttctaagga attagaaaag aaaactgctt
      121 tattaaacga aatatttagt gctgattgta taatccaaag tttaaaagaa agttcatttt
      181 caaaactaac agatcaggaa aaaatttctt atatgctaag tttagataat ctaggaacta
      241 aatatgatga atcagcaagt agcgataagc ttttgattga attagtatca agtcttagta
      301 gttttggaaa aatgaaaaga tttaatgtga tattagaaat ttttgcttgg ttaatccctt
      361 catttatttg gtttttttat attttcatag ttgaatctga gttaatagcc aaggtctctc
      421 ttggactgtt aattccgata taaataggtt cagcaattta ctttaataac ttgtatacac
      481 gtattcaaat aaaaaagcag ttattacatg aaggattaag tggtaatatg ttaaaaagaa
      541 ttgcatggga taaagataat aaaacagata ttgagcttaa agaatataaa tacaattatt
      601 tctcgagatg tatagataga aagttactgt ttaaaaaaga aggattagaa tttagttttg
      661 atgaagatga atttgaaaaa ttagtgatgg actggcgtaa gaatttactg gatacggtac
      721 aattctaaaa gcaattgagg tcgaaattcc gatacttgaa tcatgatcta tattatcgtg
      781 ttatgagtga aatttctaat aaagataaga tatttcattt ggacagtcat gtgaatatga
      841 gcttaacaat taaacgtatt tatgatcttt tatatgttta taaacattaa tgaggtgcta
      901 aaattgacaa caataactat gacaaaaaaa atagaagtat atgttgatgg atcacaagaa
      961 gaaagggatt ccttttttga ttttttttgg aaaaatagta gagaatatgt aaaagcgttg
     1021 aatctggtat ataaacagct aatgtttgaa gagttgataa aagatgcaat taaagaaatg
     1081 gatcaaggtt atgctgagag agaagaaagt attcaaaatc ggattgaaaa gttagcatat
     1141 actgatccaa tagatcaaaa aaaacttgat aaagcaaaag ctgaattatc taaattgaga
     1201 aatgaaaaaa ataaagaagc gagagaaata ttaacgcaag cgatagggtt gaagagtcaa
     1261 acgagaacac gagatgtagc aaaaaaatta gactttgact ttggagattt tgttgattcc
     1321 gcaaatatga aagctagtaa agattttggc aatgatttta tcggaattgt gaccggacaa
     1381 agaacgccta gaatatataa aaatagaaac aaatatggat tactaccttt tagaggtcga
     1441 gatattaaaa tccataaaga tgataagttt tatatttatt tacctaaagg ctttaggttt
     1501 aaagtgaatt taggtagcaa gccaagaaaa gcaagagatg ttgcttcagt tttacaacac
     1561 attatggatg gcgattacaa attaaatcaa agtaatctta tttttgatgg aagaaaatta
     1621 ttttttaatc taacaatttc ttttgaaaaa agctataatg aaatggaatt caagaaaggt
     1681 aatgaattga tcgtaaactt tgcagatttg tgtagcggtg aatgtttaat taatgataaa
     1741 tctgtaatgt cttttggtga tagccaatat ataagaaact tcaaaagtaa aattttagca
     1801 atgaaatcaa aagaacaaag ccgaggagtc ttttctaagg gcggtcatgg tcgaaaaaga
     1861 aaattagatc aagatcgatg ggaagcaata aagaaaagag aaagtaattt tgttaaaact
     1921 tataataatc aatgttctgc aaaaatcgtt aaaactgcta ttcgcaataa agctcaaatt
     1981 attcgtatca ttgaaccaga taaggcttat ggcgaatggg cttactatca attgaaagaa
     2041 caactagtaa acaaaggaaa aagatatggg atagaagtca gaagttagta aattaaacaa
     2101 tcgttgattt ggcaacgaaa tagtgaggga aaatccactc actgaagggt aaactccgtc
     2161 aagttgtgtg accagacgga aggacgaatg ttgttgaggt tttagaacca tataaaacag
     2221 catgtttctc aaatttttag gataagggaa attgctagca gtttgtttta gaaccatata
     2281 aaacagcatg tttctcaaat gagtacttta aattaattga tggcgttcct gctttagaac
     2341 catataaaac aacatggatt taaaacaaga agtaagtcag ttctcctttt actttagtaa
     2401 ggaaaaataa agtattctca ataaaactta aaataagcaa acctttgttt taatagctaa
     2461 tcgaagtaaa acccaaagcc atatgaaccc ctgaatcagc gaaagtttat gatgatttgg
     2521 attactaata gtttaggaat tagatggtaa atctaaatta aacaatcgtt gatttggcaa
     2581 cgaaatagtg agggaaaatc cactcactga agggtaaact ccgtcaagtt atgtgaccag
     2641 acggaaggac gaatgttgtt gaggttttag aaccatataa aacagcatag ttctcaaatt
     2701 gtttaaaatg attgtcaagt taaaaaaatc tgggatcata taaaatagta tagttctcaa
     2761 atatgattag aaaccctatt aaagagcaat attaagccaa cttacttaaa tcgtttaatt
     2821 aagaacaata atctctttta gtcagacttt tagtctgact tttttgtatt tccaaaattt
     2881 attctttaca aaaacgaaca tacgtttgta taattgcttg tgaatggggg aataaaaatg
     2941 tttgattaca gtagagaacc ggtgaatgac tattttttaa tcgacatgaa gtctttctat
     3001 gctagcgtgg aatgtattga acgaaattta gacccaatga ctacaccgtt aattgtcatg
     3061 agtagatcgg acaacactgg ttcaggtctg actctagctt cttcacctac tgcaaagaaa
     3121 aaatatcata ttactaatgt tagtagagct actgatttac cttatccatt accaaaagaa
     3181 cttcatattg ttccaccacg aatgaactta tatataaaaa aaatatggaa gttaatcaga
     3241 tttttagaaa gtatgtcgca gatgaagatt tattgattta ttcgattgat gaatcaattt
     3301 taaaagtgac aaaatcctta aatttattta ctagttcagg aacaagaagc gagagaagaa
     3361 gagaattagc taaaataatc aaagatgaga ttcagaaaga aacaggtcta atagcaacag
     3421 tcggaatagg agataaccca ttattagcta aacttgcact ggacaatgaa gcgaagagta
     3481 gtaaagaatt tatagctgag tggacttatg agactgtacc tgaaaaaata tggaaaataa
     3541 acgagatgac tgacttttgg ggcattggat cacgcatgaa aaaaagattg aatcatatgg
     3601 gaattatgag tatagaagat ttagcaaatt ggaatcccta cacaattaaa agtcagttag
     3661 gtgtaatcgg actccagtta tatttccata ccaatggaat tgatcgtacg gatattgcta
     3721 ttccaccaga accaaccaaa gaaaagtctt atgggaattc acaagttcta ccaaaagatt
     3781 atacgaggag agaagaagta gaactagtag tcaaggagat ggcagagcaa gtagccattc
     3841 gaattagaca acacaattgc aaaactggtt gtgtacgctt agcaattggt acgtcaattt
     3901 tggaagaaaa atcaggtttc tcgcaccaga tgaaaatacc aattacagat aatacgaaag
     3961 aattacaaaa ttattgtctt tttttattca ataagtttta tgaggggcag gaagtacgac
     4021 atataggagt aacttatagc agattagtat acacagattc cctacaatta gatttattta
     4081 gtgatccaag aaaaaagatt gctgaagaaa atttggatag gattattgat aagattagac
     4141 aaaaatatgg atttacagct attatacatg cttcatctat gttagaaggt gcaagaagta
     4201 ttacgagaag cactttaata ggaggacacg caggaggatt aggaggtttg cataatgatt
     4261 gaatataacg atagaggacg tttaaaatgg tcgggatttt atttgtctga tcatactgaa
     4321 agaatcaatg cagacaatgt acaacgtgct aatcataatt tggcaaaaga acaaatgacg
     4381 actgaagaga ttgcgaaaat actgagaact gctgtcataa aaaatagacc cgtctgcatt
     4441 caaaaagagg aacgaaacgc tgaaggttac tatccagatg atgtagtagg aaaaattatt
     4501 ggatatgacg agcttggtgt atttgtggaa gaagaaaaag ttcactatga tgaaattagg
     4561 aatgtttctt tctcggaatt attaaaatgg agtcagatat gaacattatt aaaaaatacg
     4621 aattcggtta tattacgtat gaacagctaa tagaggaatt atggggatat ggtcaacaat
     4681 taattaatga agtggggatt gattgctttt gtttctatat tgaatccgga gcaggatacc
     4741 ataagaacag atactatata tttccatatc caccaaaata gtctcttaga tatttttaaa
     4801 ttttctccaa atactttcaa ttattgtgaa atatttccct ttaattaatc aaattttt
//
LOCUS       DAGCYL010000178         4806 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.181, whole genome shotgun sequence.
ACCESSION   DAGCYL010000178 DAGCYL010000000
VERSION     DAGCYL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.181"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..411
                     /locus_tag="MB216_002438"
     CDS             <1..411
                     /locus_tag="MB216_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527221.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            509..1882
                     /gene="tet(L)"
                     /locus_tag="MB216_002439"
     CDS             509..1882
                     /gene="tet(L)"
                     /locus_tag="MB216_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBT4527222.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            2446..3708
                     /locus_tag="MB216_002440"
     CDS             2446..3708
                     /locus_tag="MB216_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBT4527223.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3953..>4806
                     /locus_tag="MB216_002441"
     CDS             3953..>4806
                     /locus_tag="MB216_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBT4527224.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1659 a          665 c          981 g         1501 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
      481 gaattaaagt gtaaaggagg aattaattat gaagtgtaat gaatgtaaca gggttaaata
      541 caaccaaatt ttaatttggc tttgcgtttt atcttttttt agcgtattaa atgaaatggt
      601 tttgaacgtc tcattacctg atattgcaaa tgattttaat aaaccacctg cgagtacaaa
      661 ctgggtgaac acagccttta tgttaacctt ttccattgga acagctgtat atggaaagct
      721 atctgatcaa ttaggcatca aaaggttact cctatttgga attataataa attgtttcgg
      781 gtcggtaatt gggtttgttg gccattcttt cttttcctta cttattatgg ctcgttttat
      841 tcaaggggct ggtgcagctg catttccagc actcgtaatg gttgtagttg cgcgctatat
      901 tccaaaggaa aataggggta aagcatttgg tcttattgga tcgatagtag ccatgggaga
      961 aggagtcggt ccagcgattg gtggaatgat agcccattat attcattggt cctatcttct
     1021 actcattcct atgataacaa ttatcactgt tccgtttctt atgaaattat taaagaaaga
     1081 agtaaggata aaaggtcatt ttgatatcaa aggaattata ctaatgtctg taggcattgt
     1141 attttttatg ttgtttacaa catcatatag catttctttt cttatcgtta gcgtgctgtc
     1201 attcctgata tttgtaaaac atatcaggaa agtaacagat ccttttgttg atcccggatt
     1261 agggaaaaat atacctttta tgattggagt tctttgtggg ggaattatat ttggaacagt
     1321 agcagggttt gtctctatgg ttccttatat gatgaaagat gttcaccagc taagtactgc
     1381 cgaaatcgga agtgtaatta ttttccctgg aacaatgagt gtcattattt tcggctacat
     1441 tggtgggata cttgttgata gaagaggtcc tttatacgtg ttaaacatcg gagttacatt
     1501 tctttctgtt agctttttaa ctgcttcctt tcttttagaa acaacatcat ggttcatgac
     1561 aattataatc gtatttgttt taggtgggct ttcgttcacc aaaacagtta tatcaacaat
     1621 tgtttcaagt agcttgaaac agcaggaagc tggtgctgga atgagtttgc ttaactttac
     1681 cagcttttta tcagagggaa caggtattgc aattgtaggt ggtttattat ccataccctt
     1741 acttgatcaa aggttgttac ctatggaagt tgatcagtca acttatctgt atagtaattt
     1801 gttattactt ttttcaggaa tcattgtcat tagttggctg gttaccttga atgtatataa
     1861 acattctcaa agggatttct aaatcgttaa gggatcaact ttgggagaga gttcaaaatt
     1921 gatccttttt ttataacagg aattcaaatc tttttgttcc attaaagggc gcgattgctg
     1981 aataaaagat acgagagacc tctcttgtat cttttttatt ttgagtggtt ttgtccgtta
     2041 cactagaaaa ccgaaagaca ataaaaattt tattcttgct gagtctggct ttcggtaagc
     2101 tagacaaaac ggacaaaata aaaattggca agggtttaaa ggtggagatt ttttgagtga
     2161 tcttctcaaa aaatactacc tgtcccttgc tgatttttaa acgagcacga gagcaaaacc
     2221 cccctttgct gaggtggcag agggcaggtt tttttgtttc ttttttctcg taaaaaaaag
     2281 aaaggtctta aaggttttat ggttttggtc ggcactgccg acagcctcgc agagcacaca
     2341 ctttatgaat ataaagtata gtgtgttata ctttacttgg aagtggttgc cggaaagagc
     2401 gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt tacatatgag ttatgcagtt
     2461 tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca tgcaatttca taatcaaaga
     2521 gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc gaacacgtga aaattatgat
     2581 ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca aagaaattat tgaatcacaa
     2641 aaaacaggta caagaaaaac gaggaaagat gctgttcttg taaatgagtt gctagtaaca
     2701 tctgaccgag atttttttga gcaactggat ccaggagaac aaaaacgatt ttttgaggaa
     2761 agttataaat tattttccga acgatatggc aagcaaaata ttgcttatgc aacagttcat
     2821 aatgatgagc aaacccctca catgcattta ggtgttgtgc ctatgcgtga tggaaaactg
     2881 caaggaaaaa atgtgtttaa tcgtcaagaa ctgttatggc tacaagataa attccccgag
     2941 catatgaaaa aacagggttt tgagttgaag cgtggtgaac gtggctctga ccgtaaacat
     3001 attgagacag ctaaatttaa aaaacaaact ttggaaaaag agattgattt tctagaaaaa
     3061 aatttagcag ttaaaaaaga tgaatggact gcttatagcg ataaagttaa atcagattta
     3121 gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc caacgggtga aaagtccatg
     3181 tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac caaccaaaaa tgttgttata
     3241 tcggagcgtg attataaaaa cttagtgact gctgcgagag ataacgatag gttaaaacag
     3301 catgttagaa atctcatgag tactgatatg gcgagagaat ataaaaaatt aagtaaagaa
     3361 catgggcaag ttaaagaaaa atatagtggt cttgtagagc gatttaatga aaatgtaaat
     3421 gattataatg agttgcttga agaaaacaag tctttaaagt ctaaaataag cgatttaaag
     3481 cgtgatgtga gtttaatcta tgaaagcact aaggaattcc ttaaggaacg tacagacggc
     3541 ttaaaagcct ttaaaaacgt ttttaagggg tttgtagaca aggtaaagga taaaacagca
     3601 caattccaag aaaaacacga tttagaacct aaaaagaacg aatttgaact aactcataac
     3661 cgagaggtaa aaaaagaacg aagtcgagat cagggaatga gtttataaaa taaaaaaagc
     3721 acctgaaaag gtgtcttttt ttgatggttt tgaacttgtt ctttcttatc ttgatatata
     3781 tagaaataac gtcattttta ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat
     3841 gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca gaaaaacccc atctgttaaa
     3901 gttataagtg accaaacaaa taactaaata gatgggggtt tcttttaata ttatgtgtcc
     3961 taatagtagc atttattcag atgaaaaatc aagggtttta gtggacaaga caaaaagtgg
     4021 aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt gattactttg aacttctgca
     4081 tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt gctgaaatat tagagtataa
     4141 acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg tggttttgta aatccaggct
     4201 ttgtccaatg tgcaactgga ggagagcaat gaaacatggc attcagtcac aaaaggttgt
     4261 tgctgaagtt attaaacaaa agccaacagt tcgttggttg tttctcacat taacagttaa
     4321 aaatgtttat gatggcgaag aattaaataa gagtttgtca gatatggctc aaagatttcg
     4381 ccgaatgatg caatataaaa aaattaataa aaatcttgtt ggttttatgc gtgcaacgga
     4441 agtgacaata aataataaag ataattctta taatcagcac atgcatgtat tggtatgtgt
     4501 ggaaccaact tattttaaga atacagaaaa ctacgtgaat caaaaacaat ggattcaatt
     4561 ttggaaaaag gcaatgaaat tagactatga tccaaatgta aaagttcaaa tgattcgacc
     4621 gaaaaataaa tataaatcgg atatacaatc ggcaattgac gaaactgcaa aatatcctgt
     4681 aaaggatacg gattttatga ccgatgatga agaaaagaat ttgaaacgtt tgtctgattt
     4741 ggaggaaggt ttacaccgta aaaggttaat ctcctatggt ggtttgttaa aagaaataca
     4801 taaaaa
//
LOCUS       DAGCYL010000179         4799 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.182, whole genome shotgun sequence.
ACCESSION   DAGCYL010000179 DAGCYL010000000
VERSION     DAGCYL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.182"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..770)
                     /locus_tag="MB216_002442"
     CDS             complement(<1..770)
                     /locus_tag="MB216_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4527225.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTI"
     gene            complement(886..1521)
                     /locus_tag="MB216_002443"
     CDS             complement(886..1521)
                     /locus_tag="MB216_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBT4527226.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLILLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTAKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(1604..2026)
                     /locus_tag="MB216_002444"
     CDS             complement(1604..2026)
                     /locus_tag="MB216_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBT4527227.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFETEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(2044..2364)
                     /locus_tag="MB216_002445"
     CDS             complement(2044..2364)
                     /locus_tag="MB216_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4527228.1"
                     /translation="MNPYEKIKELTKQNGISVRELEKRLGYSNGYFSKWKSVSPNSEG
                     LAKVSDYFGVSIDYLLGRDKKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            2665..3441
                     /locus_tag="MB216_002446"
     CDS             2665..3441
                     /locus_tag="MB216_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBT4527229.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            3456..3659
                     /locus_tag="MB216_002447"
     CDS             3456..3659
                     /locus_tag="MB216_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4527230.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            3675..4013
                     /locus_tag="MB216_002448"
     CDS             3675..4013
                     /locus_tag="MB216_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBT4527231.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            4000..4170
                     /locus_tag="MB216_002449"
     CDS             4000..4170
                     /locus_tag="MB216_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527232.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYKTKKPVGRD"
     gene            complement(4268..>4799)
                     /locus_tag="MB216_002450"
     CDS             complement(4268..>4799)
                     /locus_tag="MB216_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527233.1"
                     /translation="KKFNLKLDFFAECEEWLIDRESDSKPDVYHQNKFRIIDSVLLTN
                     NSSLPVTIIEFSISGIPDPLNAFTMIGDNYSVTVKSMYDELPHGIRAYSGKSLKKGAD
                     LSKFPPLSLPITIPPYESKITTLVFRYDESLVEKNITINVLTSRGTAKFNRFVSSSQI
                     SQLDTGYAPPQLDEFD"
BASE COUNT         1612 a          795 c          783 g         1609 t
ORIGIN      
        1 tcaatcgtat gtttatcaat ggaaattttc ctatttgaag tttcgttttt tgtttttccc
       61 cattcattta atttataatt ccatgtttta ttaatagtga tgatttgatc ttcgaaatcg
      121 atatcttccc atgttaatcc tagaagttca gcaaatcgca ttccagtagc accagcaact
      181 aacacgagca taggagaaga gtactgcgca gaaaggtttt cttctacgag tttcattaaa
      241 gatttaaatt gatcataatc taaatattta tcttcttttg gcttcaatga atcttttcct
      301 ttgattactg cttttctagt tggatcaaac ggaattaatc cttcttctac ggcatctttc
      361 aacgatgctc taatatggtt attaaactta ataacggtgg attttacatg gtctttcgca
      421 tacttattta aaaagcgttg atatccaact ctatctaaat cagaaatcaa taccgctggc
      481 atatatttct ttatgttcat taacgtatct tcgtattttc tataagtgat aggagatact
      541 gttccttctt tataaagttg catccaatcc tcaaagtaat ctgataataa tagatttttg
      601 cgatcccctt taaggccttt gttcaagtta tattctaatt cgttggcggc atcttttgct
      661 tcgccttttg tcttaaatcc tgattttctt atcttcgtgt attttccgtc atcttttttg
      721 tatgaaattt cgtattgcca actatttcca cgtttgacta atcttgccat aattgaatca
      781 gctctctttc tctgatacaa taggaactat aaagaagcct actgtatagg tttgtttttt
      841 catagaacac gcttgctttg gacggtgggc gtgtttttat atcaattagt tatgaagagc
      901 aattgaaact gtctctgttc caaagtaacc tgtttgtgct tgcaatacat tattttgtgc
      961 atttgcttgg gcttcagaga tatcaaaaac tattttacca gtttgttgca tatcaggatt
     1021 taagctttcc ataaagaaag agttagtgat attaccattt tcatcttgat ttgccgacat
     1081 agatgcagcc gaatcagctt cgaaagtttt tccatctgct tttaatttga agaatgagct
     1141 atcaacagtc acagccttat cacctgcatt ttttacagat aagtcaacta ctaagaaagt
     1201 gtcttttgcg ttagtaggaa gtacagatgg gccaacttgt tttactactt caacggagtt
     1261 tactttgtat tccattttcc ctacagcaac gtcatcaccg attttataag tcttttcttc
     1321 ttttgctgta gtttcttttg tagtttcttt attagtactt gttgatgttg cagtagctga
     1381 atctttgttt tctccgccac ttagagcacc accaatacca aaaaacaaaa gtaatattaa
     1441 tacccaaaac cacacttttt tgtaaaaagg tttactaact ttatacattt tcccatcttg
     1501 acccataact ttttttgcca tttaaatatt cctcatttct tgttataata tatttgtgat
     1561 ctcagaaatg aggtatgagt ccgtgttgca gcacgggctt tttttactgt gcataagagt
     1621 attttttctt gaaataagac tggcaaacat taaaacattc tgttcttaac ttattattga
     1681 tagaatagaa ctccatgaaa ttttctaatt tgaactgaga ttcatcagtc agttcgttct
     1741 caataaagat atttagtaaa atcataattg cgattctatc agcttcagtt tcgaattttg
     1801 aatgaaaagt tgtagagtta tcgtacagta ctgaatattc aaaatgtgaa gcaatgaaat
     1861 gaccgagctc gtgggctaaa tgaaaagctt cagaactgtc ttcgtgtagt ttttcattca
     1921 gaaatactat tcttggtttt ggataataaa aacctggttc ttccatttcc atatagatta
     1981 actttaaatt atactcactc agcatttctt tcaactttaa atacatacaa cccatcactc
     2041 caactattca ttttcctcta aagctttagc aattgcaatc gctttacgca ttgtctcctt
     2101 agatatttct tttccatcaa aagaaaaaac agtatcgtct tctgataaat ccacatgttt
     2161 aggggtttct tttttatctc ttcctagaag atagtctata gaaacaccaa aataatctga
     2221 tacttttgcc aaaccttcgg agttaggtga tactgatttc catttactga aatatccatt
     2281 tgaatatccc aatctttttt ctaattccct tacggatatt ccgttttgct ttgttaattc
     2341 ttttattttt tcgtatggat tcattgatat gtcaaccttt ctagactgac aagaaaaaat
     2401 atagaataaa actctattta tctgttgaca aattagagtt aaacctctat acttgttctt
     2461 gtaaacaaat ttaacaacta aaaagacaac aaaaaacact tttgattatt aaacgccaac
     2521 cgccaaaaaa gcttttaaaa tcaatgttta tatgtcttat ttaactatgt acaaagtata
     2581 gaataaaact ctatgctttg tcaattgaat ttagaaaaaa gttgttaaat ttgtttacga
     2641 atataaaaga aaggaatgag aaatatgaac acaccgcaaa tttttaattt cgaacaaaac
     2701 gaagttcgga caattttagt aaatgatgaa ccatattttg taggcaaaga cgttgcgagt
     2761 gttttgggtt attcaaacac taaagatgct ttgtcgcggc atgtagattt ggaagataag
     2821 atggggtcgc gaatcacgac ctcaggtcaa tcaagagaga tgacaatcat caacgaatct
     2881 ggtttgtaca gtttaatctt aaaatcaaaa cttccctctg ccaaaaaatt taaacgttgg
     2941 gtaacaagtg aagtgttgcc agcaattaga aaacatggag gttatctaac tccagaaaaa
     3001 gtagaagaag ctttgcttaa tccagataca atcattcaat tagcaactca actaaaagaa
     3061 gaaagaactg gaagattaat cgcagaacaa aagattgcag agtacgaacc taaaatctcc
     3121 tatttagata gcatattatc ttctacagat tcagtaacaa ttagtcagat tgcagcagat
     3181 tatgggatgt ctccacaaca gatgaataaa ttacttcata aactaggtat tcagaaaaaa
     3241 gtcggtaacc aatggttatt gtgcaaaaaa cacatgaacc aaggatacac aaaatctcat
     3301 acaactgaga tcccgaaagc cgatggtggc actaaaattg taatgaatac caaatggaca
     3361 cagaaagggc gtctatttat ctacgaatta ctaaaaaaag aaggatatta ccctcaaatg
     3421 gatttagagg aaattggtta gaaaggagtt ttagtatgac tgacattgca gaaatcactc
     3481 aacgagatag agaaaaaatc aaagaatatg tcgaaagttc aaagttctta acttacacca
     3541 tgctcgctga aagattcggc attagcaaaa gctacttatc tttaatttta aacggtaaaa
     3601 agacttctgc agaagcaaat agaatcatag attcgattat tactatgtac gaattgtaaa
     3661 gggaggaaca gctaatgcaa tatctagaag cgaaaatccc agttccagaa ggctatgtaa
     3721 ttatctccca agtggattat gaggagttaa aaaaagctga tgatactggt agatggatga
     3781 cgttgccaga agtgctagaa cggattaaca gaaaatatga ttggtttact tctagagttt
     3841 taaagaaccc aagatataga aatattatcg atatcgaaaa aaacaaaaat ggatttgtct
     3901 attatccagt tgaaggaaga gacacatatc tatttttaag aagtaaaaca cttgaatttt
     3961 tagaaacaaa tttttcggaa atcttaagga ggcaagcgga tgggaaaatt taatagagcg
     4021 ctagtattca gcgcaccgct aatcatctac gctttaggac tttggggaag cagacaagcg
     4081 ttgataggaa cgattgttta catggtttgg atttttatgg ggctggatga agctgagtac
     4141 aaaacaaaaa agccagtcgg gagggactga ctaatgaaaa aaagtttaat gactataaac
     4201 gaaaaacaat tgaaagaaaa atttaatgat ctcatcaaag aatttgtaaa agaaactgga
     4261 gaatttccta atcaaattca tctagttgcg gaggggcata gccggtatca agctgtgaaa
     4321 tttgagatga agaaacaaat ctattgaatt tagcagtacc tctacttgta agaacattta
     4381 ttgtaatgtt tttttcgact aaagattcat cgtatctaaa aaccaacgta gtaatttttg
     4441 actcatacgg tggaatggta ataggtaatg acaacggagg aaattttgat agatcagcac
     4501 ctttttttaa agatttacca gaatacgcac gtataccatg tggaagttcg tcatacattg
     4561 attttaccgt tacagaataa ttatcaccaa tcattgtaaa agcatttaaa ggatcaggaa
     4621 ttccagaaat cgaaaactca ataattgtaa caggtaaaga actgttattt gttaacaata
     4681 cagaatctat aatcctgaat ttattttgat ggtaaacatc tggtttggaa tcactttctc
     4741 tatctatcaa ccattcttcg cattcagcga agaaatccaa ttttaaatta aatttttta
//
LOCUS       DAGCYL010000180         4786 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.183, whole genome shotgun sequence.
ACCESSION   DAGCYL010000180 DAGCYL010000000
VERSION     DAGCYL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.183"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(81..1889)
                     /gene="pepF"
                     /locus_tag="MB216_002451"
     CDS             complement(81..1889)
                     /gene="pepF"
                     /locus_tag="MB216_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBT4527234.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2065..3063)
                     /locus_tag="MB216_002452"
     CDS             complement(2065..3063)
                     /locus_tag="MB216_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBT4527235.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3289..3942)
                     /locus_tag="MB216_002453"
     CDS             complement(3289..3942)
                     /locus_tag="MB216_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBT4527236.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="MB216_002454"
     CDS             complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="MB216_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBT4527237.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1404 a         1007 c          744 g         1631 t
ORIGIN      
        1 aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag
       61 aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt
      121 tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct
      181 tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc
      241 tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag
      301 ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg
      361 aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca
      421 taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg
      481 aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag
      541 taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc
      601 gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg
      661 tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg
      721 acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg
      781 tcatagcttc ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc
      841 caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt
      901 gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag
      961 accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat
     1021 tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat
     1081 acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg
     1141 actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt
     1201 ctaaattgtt cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc
     1261 gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc accattttct
     1321 ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa
     1381 gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg
     1441 gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct
     1501 ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa
     1561 acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct
     1621 gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg
     1681 cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt
     1741 gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca
     1801 aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct
     1861 tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta
     1921 tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca
     1981 ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg
     2041 attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt
     2101 aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg
     2161 ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc
     2221 ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt
     2281 ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg
     2341 tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc
     2401 ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat
     2461 ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga
     2521 tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca
     2581 gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg
     2641 cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata
     2701 attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc
     2761 tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa
     2821 tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc
     2881 ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc
     2941 acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt
     3001 ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag
     3061 catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc
     3121 gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat
     3181 ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa
     3241 aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata
     3301 aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt
     3361 ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac
     3421 atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa
     3481 taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg
     3541 ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga
     3601 taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc
     3661 tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat
     3721 aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg
     3781 gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt
     3841 gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat
     3901 tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt
     3961 gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa
     4021 tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca agctatctta
     4081 tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc
     4141 ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat
     4201 actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt
     4261 tcgtattatc cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta
     4321 catcgcgcgg taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat
     4381 catcaatcat gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt
     4441 cttgtaacgg cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg
     4501 agatgatttc ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt
     4561 tcaatggttc tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg
     4621 cacgagcctt tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc
     4681 actccttttc gtgataagaa tcactttttt gtaacagaag atcataataa tctcccatct
     4741 gtttctatta tacataatca acttttaaat agctaccttt ttttca
//
LOCUS       DAGCYL010000181         4718 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.184, whole genome shotgun sequence.
ACCESSION   DAGCYL010000181 DAGCYL010000000
VERSION     DAGCYL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.184"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            149..1210
                     /locus_tag="MB216_002455"
     CDS             149..1210
                     /locus_tag="MB216_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBT4527238.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1213..2109
                     /gene="murQ"
                     /locus_tag="MB216_002456"
     CDS             1213..2109
                     /gene="murQ"
                     /locus_tag="MB216_002456"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBT4527239.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2123..4105
                     /locus_tag="MB216_002457"
     CDS             2123..4105
                     /locus_tag="MB216_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4527240.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4170..4616)
                     /gene="nrdI"
                     /locus_tag="MB216_002458"
     CDS             complement(4170..4616)
                     /gene="nrdI"
                     /locus_tag="MB216_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBT4527241.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         1533 a          828 c         1055 g         1302 t
ORIGIN      
        1 ttttaggtta tgacaattct tttgataata taaactcaac ctttagaaat gaaatttaat
       61 tattttaata aaagtattga aattttgttt ctttagatat atactctagt catagaaatg
      121 aagacggata cagaaaaaga gggataatat gtttggcttt tcaatattta tgaacgattc
      181 tttgtcagaa gagaagaaac agtatatccg ccaaatggca tccaatggct ttgtcggtat
      241 tttcacttcc atgcatatac cagaagatga tgtttcggca tatcaaaagc gattgatcga
      301 actaggaagc gaagcgaaaa agaataaact agaattgatg gtcgatattt caggagaggc
      361 attggaacga gcgggttttt cattagaaga tctaacgcct ttgaaagcaa ttggtgtaac
      421 aggtttaaga atggattatc atatttccaa tcagcagatt gccgactggt cacatcaatt
      481 gaaaatcagt ttgaatgcta gcacgatcac gcctaaagat atcgatgaat tgaaagaagc
      541 agaagctgat ttctcacaga ttgaagcttg gcataattat tatccacgtc ccgaaacagg
      601 gctggacaaa gaatggtatc agaaaaaaaa tcaatggctc aaaattcaag gtctccttgt
      661 ccaaggtttt gtaccaggag atacagagtt gagaggccca ctatatcaag gattgccaac
      721 tttggaagaa catcgaggga tccatcctct tgcaggcgct ctagatctat tagcaagtaa
      781 cacggatatt gtttacatcg gagatgcagg tctgtcagaa aacgttcagg agcaatttgc
      841 ttctttccaa aaagaacaga cagtcttgct ccatacggaa cctgtagatg aagaatttta
      901 cgaatatata ttagggaagc atacgaatcg gcaagacgat gcaagagatg tcatacgaag
      961 cgtagatgca cgttttcgag agattccgcc tatcccagca agaaatacag ctacgcgtat
     1021 gaaagggtct attactttag ataacgaaaa gtatttgcgc tatatgggtg agatccaatt
     1081 aaccaaatat gatttgccag cggatgaaaa agtgaatgta gtagctaaag ttatcaaaga
     1141 agagctacca ttgatcaacc aaatcaaagc tggtatgaac tatcaattta taagaaaaga
     1201 aggaagataa aaatgagcaa aatcaattta gagaacctaa caacggaacg cagaaaccaa
     1261 gaaacttttg gattagatga gatgagtatt tctcaagccg ttcaactaat gaacaaagaa
     1321 gatcagaaag tggccgctgc ggttgaaaaa gaactgacaa gtatcgaacc agtgattgct
     1381 aacacgattg agtcctttaa aaaaggtgga cgtctgattt atatgggagc cggaacaagt
     1441 ggaagactag gtgtgttaga tgctgctgaa tgcgtaccga cattcggtgt agaaccggaa
     1501 atggtcatag gtctaattgc tggtggagaa gaagcaatga ctgtagctgt cgaaggggcc
     1561 gaagattctt atgagctagg gcaagaagat ctaatcaagc tgaatttgac aaaaaatgat
     1621 atagttattg gtatcgcagc tagcggtcgt acaccatatg tgatcggtgg attagattac
     1681 gcaaaatcga tcggtgctgt cacaggaacg atttcttgca ataaacaagc cgaaatcagt
     1741 aaacacgcgg atttcccaat cgaagtcgat gcaggtccag aatttttgac aggctctact
     1801 cgattgaagt caggaacagc acaaaaactt atcttgaata tgatttctac tatcagtatg
     1861 attggtatag gtaaagtatt caataacttg atggtcgatg tcaaaccaac aaatgaaaaa
     1921 ctaatcgaac gctcaaaacg aatcatcatg caagcgacag aagctgatta cgaaacagct
     1981 gaaaagtact ttgaagaagc agatcaaaat gttaagctgg ctatcgtcat gattttgaca
     2041 aatagtgata aagaggaagc tcaagcaaaa ttagctgaag caaacggatt tgtaaaaggg
     2101 accttatgag ggggaaaaga ccatggcaaa aaacgaactg actttggaag aactggcaaa
     2161 acaaatttat aaaggggcag gcggcatggg caatgtagaa agcattgtcc actgcatgac
     2221 tcgtgtgcgt atgaagatca tcaatgatga tcttgtaaat gtctctgaat taaaagcgat
     2281 tccaggagtg cttggtgtag tagaagatga acagcttcaa gtcatcatcg gacctgggaa
     2341 agtgaacaaa gtagcacagc aaatggttga tttggcaggc gtgaaattag gagaaaaact
     2401 ttcttctgta agtacagcta cagcaaattc atcagcatct ggaaaagacc gtgtaaacga
     2461 acgggcacaa gaaatgaaag cggcacaaaa atcgaaacaa aaaccatcta aaatcaaatc
     2521 ggtattaaaa gacatttcta atatctttgt gccaatgatt cctgcatttg tcggtactgg
     2581 gatcgtggca gggattgccg cagttttagc aaacttagtc acagccggaa cacttgatgc
     2641 agcaacatgg caacaatata tcgatgtgat gaacatcttg aaaaacggga tgtttagcta
     2701 cctagttatc tatacaggta tcaatgctgc acaagttttt ggtgcaacac caactcttgg
     2761 tggagttatc ggggccgttg tgatgctgac agggatgaat ccggaagcac cactgaaaaa
     2821 cttatttact ggtgaagctc ttgcagctgg acaaggtggg atcctaggtg tcatcttcgc
     2881 tgtttggcta ttgtcaatcg ttgagaaaaa actgcataaa gtagtacctg atgcggtaga
     2941 tatcatcgtt acaccaactc tttcattgat tgccatcggt ctgattgaaa ttttcttcat
     3001 catgccttta gcaggtttca tttctgatgg catggtcggt gggatcaact gggtacttgg
     3061 cgttggtgga gcatttgccg gcttcgttct aggcacatta ttcttgccga tggttatgtt
     3121 cggactgcat caaattttga ctccaatcca tgtgcaaatg attgatgaaa ctggtcgtac
     3181 gctattgctt ccaattcttg cgatggctgg tgcaggacaa gtgggtgcag cattggcatt
     3241 atggattcgc tgcaaaaaag ataaagaatt aacagaaatg atcaaaggag ctttaccagt
     3301 cgggatccta ggaatcggcg aaccattgat ttacggagta acattacctc taggacgccc
     3361 atttattact gcttgtatcg gtggaggtat cggcggggca gtgatcggaa tgatcggaaa
     3421 tgtcggggct atcgctattg gaccttcagg agccgctttg atcccattga tctctgacgg
     3481 taaatggtta ggctacatcc ttggattatt ggcagcttat gctggtggct tcgttgctac
     3541 attcttcttc ggtattccta aagaccgttt agaaaaagaa gaattagcga ctgaaacagc
     3601 cacagtaaat gaagtgcctg aaaaagtagt acctcaaact tcagctgaaa gtgtgactat
     3661 cctctcatca gttgctgacg gaacagttgt ttctttagat aaagcaagtg atccagtttt
     3721 tgcacaaaag atgatgggtg aaggctactt tgtagaacca gaaaacggac atatctattc
     3781 tccagttact ggggtagtca gctctgtctt cccaacgaaa cacgctattg ggatcacaac
     3841 gccaaatggt ttagaaatct tgcttcatat gggaatcaac acagttgact taggcggtac
     3901 gccatttgat gtgaaagtaa cagaagggca gcaagtgaca cctgatacgc ttgtagcaga
     3961 agctgactta gaagcgatca aagcagcagg caaagaaaca tccatgatcg tcttggtaac
     4021 caatatggat cgtgtaaaaa actttgtctt ggaaaaaaca ggtaaagcaa aagcaaaagc
     4081 acctgtgatg gatgtcaaag cttaatatga tagaaacaat agaaacgaaa gaagaaactg
     4141 aaatctattt ttcagcttct tcttttttat tatttattgt tttctgctaa aatctgataa
     4201 attcgtgtca catcagatgg tgttccccgt aattcataat ctgccaagaa aggaaagcca
     4261 aatgtttgtg catattgttt ggctgttaga caatactgat tattgaagtt tttatttccg
     4321 ctaccgacta caccaagaca aagtttatga ttgtcttcat gttccagata ttcacgcatc
     4381 gtttcggtca agatttctgt atcaccgtta tctaaaccat ttccgccgtc taaataagta
     4441 ggaacgaaag taaaaaatgg ttctgtttct ttcgtaaaat cactattttc atggatttct
     4501 tttaaagtaa cttcaggatt gacaggatct tctgcatgca ttttttcagc atattctgct
     4561 agatgtttgg caaaggcacg cgtatttcct gaaatcgata tatataaaat gttcatggat
     4621 catcctccaa ttctttcctt ttagtatagc atctttttta agttttttgg tggtgtaatt
     4681 tggaaaggga ctaggcgctt tgcttgtata atgagaaa
//
LOCUS       DAGCYL010000182         4705 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.185, whole genome shotgun sequence.
ACCESSION   DAGCYL010000182 DAGCYL010000000
VERSION     DAGCYL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.185"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(274..1464)
                     /locus_tag="MB216_002459"
     CDS             complement(274..1464)
                     /locus_tag="MB216_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBT4527242.1"
                     /translation="MNYFTTSLMKNLFSNDNNLPAFEQAVTELFRSELEKSLNEILAY
                     ELTDFLDYEPYARTNNENSRNGSYQRKFDTKYGTITLNIPRDRMGEFFTSLLPKYRRR
                     DLFTETTILDLFEEGMSHSEICSMIQKLCGARYSKQTISNITDKALECVDSFKNRILN
                     KEYAVVYLDGTSMPLRRDTVSREMVHIALGITVEGTKEILGYMIAPNESAEIWEELLK
                     DLKSRGVERISLFCTDGLSGMENVIQDIFPGSKIQRCLVHIQRNICAKVRVSDRKEVA
                     NEFKEVYRSKSKEEALTSLKKFIDRWKHKYPSMVKSLSNNDYLFTFYDYPESVRHTIY
                     STNLIEGNNKQLKRSFKKKEQFPTEKSEEKYLVIQFNRYNEKNMNHVHRGFGQTTRED
                     WFKS"
     gene            complement(1863..3164)
                     /locus_tag="MB216_002460"
     CDS             complement(1863..3164)
                     /locus_tag="MB216_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4527243.1"
                     /translation="MENSAFIMKLNDILSPIAAKIGNQRHLKAISSGMMFGLPFIVIG
                     SFFLIFANPPINIDKYNPDTANIFMKFMASWKDFAVANYDLITAPYNLTMGIIGFISV
                     FGIAFSLANEYKINPSMNGMVASVIYLMVCTPVEEGAINLNFLGTNGLFVAIIIGLLV
                     VEINRLFEVKNIKLKLPDTVPPMVATFINMLVPLLTNIFIFYGINLIFLAATNKIFPE
                     AVMEVLTPATNIAGSLGGFLLIVTLGNILWLFGINGSSIIFPIVFTLGMAQTGLNAEQ
                     LANGETMTHLMNLQMFRISVLGGAGGTLGLVLLMMRSKVPEYRTIGKLSLVPGICSIN
                     EPVIFGLPIVFNPILAIPFLITPIISLLLTYFAQQLGWIGIGFIVDPSFTPFFAQAYL
                     SSMDWRNILFCLLLIVISIFIYYPFFKVMESNKTKLVDEKL"
     gene            complement(3176..3793)
                     /locus_tag="MB216_002461"
     CDS             complement(3176..3793)
                     /locus_tag="MB216_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527244.1"
                     /translation="MTETKFIQDPWSKMTTRNGLRGDEAISALQKSIRRGKEKEACEF
                     AYEMYITSPQFEEKLWRRLLAISVEDVGMGNPMAAIMINNLNAMRKEFQYNESDRAMY
                     FFHAIRYLCQSEKDRSSDLLKNIVIKSFAMGYIPEIPDIALDKHTVRGQAMGRGSEHF
                     LHEASKVIPQAKVDNDYKEQYEKIIETYDPDHVVPSTFSFNPWQV"
     gene            3834..4526
                     /locus_tag="MB216_002462"
     CDS             3834..4526
                     /locus_tag="MB216_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4527245.1"
                     /translation="MDVEMLIDKYQLNDSEAEVLRFMKKNRQSLKNIGIRDVAKASYV
                     STATIINMAKKIGYSGYSELVFALGNSQSDITMPSTFSNEEKEAFIHLMSAYRDKRIM
                     ILGSGFSQNLANYFSESLNLYGFRATANSHLEFLRQQVQQDILLFIISNSGDTVRLAE
                     LAKIANDHFIDVIAFVGQKNSKIGTLATLTISTETYIPQKISDYQPNLFFGTTLNQFE
                     LLLSAALKAIYK"
BASE COUNT         1463 a          844 c          842 g         1556 t
ORIGIN      
        1 aagtaaattt atttaataat aaacaaaatt tttcagcttt tttattcttg gaataacgag
       61 gtattgtttt cgataaaaaa agatggagca gacaaattat gataattaag catactatca
      121 gataactcaa aatgaagcct tctttcaaaa aattattcaa aaccatatat tgtatttttt
      181 atttgataat aacaatggga ttattaagaa gtggcactaa ataaaaggga gcgtcaagaa
      241 ttatgtgtaa aagtatatcc ttggtaaagc agattagctc ttgaaccaat cttctctggt
      301 tgtttgtcca aaacctctat gtacgtgatt catgtttttc tcgttgtaac gattgaactg
      361 aatcacaagg tatttctctt cagatttttc cgttggaaat tgttctttct ttttgaaact
      421 tcgttttaat tgtttattgt ttccttcaat cagatttgtt gaataaatcg tatgcctaac
      481 actttctgga taatcataga atgtaaacag atagtcattg ttgctgagac ttttcaccat
      541 tgatggatat ttgtgtttcc acctatcgat gaatttcttt agagaagtta aagcttcttc
      601 ttttgatttg gaccgatata cctctttaaa ttcattggcc acttcttttc tgtcactgac
      661 tcttactttt gcacatatat ttctttgaat atggaccagg catctttgaa ttttagagcc
      721 cgggaaaatg tcctgtatta cattttccat cccagacaat ccgtctgtac aaaataaaga
      781 aatacgttca acccctcttg atttaaggtc tttcagaagt tcttcccata tctcagcgga
      841 ctcgtttggt gcaatcatat agccaagaat ttcttttgta ccttctactg tgattcctag
      901 tgctatatgg accatttccc tggatacagt atcacggcgt aaaggcatgg atgttccatc
      961 aagatataca acggcatact ctttgttcaa tattctgttt ttgaaggaat ctacacattc
     1021 cagagctttg tccgtaatat tagatatggt ctgtttagaa tagcgagctc cacataattt
     1081 ttggatcatg gaacaaattt ctgaatggct cattccttct tcaaataaat ccaaaatcgt
     1141 tgtttcggta aatagatctc gtcttctgta tttaggcaga agagaagtga aaaattctcc
     1201 cattctatct cttggaatat tgagtgtaat agttccatat tttgtatcaa actttctttg
     1261 ataggaacca tttctagaat tttcgttatt ggttctagcg tatggttcat aatcaagaaa
     1321 atccgtcaac tcatatgcga gtatttcatt gagagatttc tccaactctg aacgaaacag
     1381 ttcggttaca gcctgttcaa aagctggaag attgttgtca ttagaaaata ggtttttcat
     1441 taaagatgta gtaaaatagt tcatgggtat attcctttcg taaattgttt ttggtcgaac
     1501 taattttact aaggatatac ctttttttct acatttctag aaaaacgaat ttacacaaga
     1561 tattttatgc tatccattta tttttgaaac tccatttgtt ttactacgtg tattaccttt
     1621 atttttcttt ctatttttag gtgtctatta tctcttacgt tcgcttataa atgatgactc
     1681 aaaatttaaa gcaaaataaa aataaaggaa gtagcctttt gaacaagggc gcttccttta
     1741 tttgttgtgg gacaccagag tatacttttt tataaaaaat aggttgatgg gaaaccctaa
     1801 gatttcccat caacactaaa tagtatagaa ccatattttc gttacaaggt taagaatgac
     1861 gactatagtt tttcatctac taattttgtt ttattgctct ccatgacttt aaaaaatgga
     1921 tagtagatga agatgctgat gacgatcaac agtaggcaaa agagaatatt gcgccagtcc
     1981 atacttgaaa gataagcttg tgcaaaaaaa ggtgtgaagg aaggatcgac gataaatcca
     2041 atcccaatcc atcctagttg ctgagcaaag taggtcagca ataatgaaat gatgggcgta
     2101 atcaaaaatg gaattgccaa aatagggtta aaaacgatag gtaatccgaa aatcactggt
     2161 tcgttgatgg aacagattcc aggaaccaat gacaattttc caattgtgcg atattcgggt
     2221 actttgcttc gcatcatcaa taaaaccaaa cccaaagtac caccggctcc accaagtaca
     2281 gaaatacgga acatttgtaa gttcattaag tgtgtcatgg tttcaccatt tgccagttgt
     2341 tcagcattca aaccagtttg agccattccc aatgtaaaga cgatagggaa aataattgac
     2401 gagccattga taccaaatag ccataggatg ttacccaaag tcacgatcaa taagaaacca
     2461 cctagactgc cagcgatgtt cgttgctgga gtcaggactt ccatcaccgc ttcagggaag
     2521 attttgtttg ttgctgccaa gaaaatcaga ttgatgccat aaaagatgaa tatatttgtc
     2581 agcagtggaa cgagcatatt gataaaggtt gctaccatgg gtggaactgt atctgggagt
     2641 ttcaacttaa tattttttac ttcaaatagt cggttgattt ccactactag caagccgatg
     2701 ataattgcga cgaacaatcc gtttgtccca aggaaattaa gattgatggc accttcttcc
     2761 actggtgtac aaaccattag ataaatgaca gatgcaacca tgccattcat ggaaggatta
     2821 attttgtatt cattggcaag ggaaaatgca atgccaaaaa cactaatgaa tccgatgatt
     2881 cccattgtca aattataagg ggctgtgatg agatcataat ttgctacggc aaaatctttc
     2941 cagctggcca taaatttcat gaaaatatta gctgtatctg gattgtattt atcaatattg
     3001 atgggtggat ttgcaaaaat cagaaaaaaa gaaccgataa caataaaggg taggccaaac
     3061 atcataccgc tggatatagc ttttaggtgg cgctgattac ctatcttggc tgcgatgggg
     3121 ctaagtatgt cattcagttt catgataaac gcagaatttt ccatataact tcctcctata
     3181 cttgccaagg attaaatgaa aacgtacttg gcacgacgtg atctggatca taggtctcga
     3241 tgattttttc gtattgttcc ttgtagtcat tatcaacttt tgcttggggg atcactttac
     3301 ttgcttcatg caaaaagtgt tctgaaccac gccccattgc ctgtccccga acagtatgtt
     3361 tatccaacgc aatatcagga atttctggaa tatatcccat agcgaaactc ttgataacaa
     3421 tgttcttaag taaatccgat gaacgatctt tttcagattg gcataggtaa cgaatcgcgt
     3481 ggaaaaaata catcgcacga tcggattcat tgtattggaa ttctttgcgc atggcgttta
     3541 agttattgat catgatggcg gccataggat tgcccatgcc cacatcctca acactgatcg
     3601 ccaatagcct tctccagagt ttctcttcaa attgtggtga ggtgatatac atttcataag
     3661 caaattcaca tgcttctttt tcttttcctc gacgaatcga tttctgtaat gctgagatag
     3721 cttcatctcc acgtaagccg ttacgggtag tcatttttga ccacggatct tgaataaatt
     3781 tcgtttctgt cataatataa tactcctatt agataaaatt tgaggtgaat cttatggatg
     3841 ttgaaatgct tatagataaa taccaactga atgactcaga agctgaagtc ttacgattta
     3901 tgaaaaaaaa tcgtcagtct ttaaaaaata ttgggattcg tgacgttgcc aaagcatcgt
     3961 atgtttctac tgcaacgatt atcaatatgg cgaagaaaat tggatattcc ggttatagtg
     4021 agttggtatt tgctctagga aacagccaat ctgacattac gatgccaagc acattctcca
     4081 atgaggagaa ggaagcattc atacatttaa tgtctgcgta tcgcgataaa cggattatga
     4141 ttcttggctc aggtttttcg caaaatctag ccaattattt ttcggaatct cttaatctat
     4201 atggatttcg tgcgacggcc aatagtcatt tagaatttct tagacagcaa gtgcaacaag
     4261 atattctgct ttttattatt tccaattctg gtgatacagt tcgattagcc gaattagcta
     4321 aaattgcgaa cgatcacttt atcgatgtga tcgcatttgt tgggcaaaaa aattcaaaaa
     4381 tcggtacgct ggcaacatta accattagca cggaaaccta tattccacaa aaaatatctg
     4441 attaccaacc aaatcttttc tttggcacga cactgaatca atttgagctt ttgttgagcg
     4501 cggcgctgaa ggctatttat aaatagaaga acgctgtggc cactgtttct tcaaaatcta
     4561 gtataaagaa tcttctttag aatgtaattg ttttctgtgt gttctgaaca tgtttaggaa
     4621 tttccaaact tgtttagaaa actttctcat tccctccgct gtagaattga actcacataa
     4681 aaaacatcta aaccaaaata tctac
//
LOCUS       DAGCYL010000183         4663 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.186, whole genome shotgun sequence.
ACCESSION   DAGCYL010000183 DAGCYL010000000
VERSION     DAGCYL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.186"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(83..1018)
                     /gene="murB"
                     /locus_tag="MB216_002463"
     CDS             complement(83..1018)
                     /gene="murB"
                     /locus_tag="MB216_002463"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBT4527246.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1091..2011)
                     /locus_tag="MB216_002464"
     CDS             complement(1091..2011)
                     /locus_tag="MB216_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4527247.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2124..2876
                     /gene="xth"
                     /locus_tag="MB216_002465"
     CDS             2124..2876
                     /gene="xth"
                     /locus_tag="MB216_002465"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBT4527248.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3862..4395
                     /locus_tag="MB216_002466"
     CDS             3862..4395
                     /locus_tag="MB216_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBT4527249.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1376 a          914 c          837 g         1536 t
ORIGIN      
        1 gcgccatttg cgtaaacaac cacaaaaaag agcaggacgg ccaccgtcct gctcttttac
       61 ttattttaag ttccattact tattaattgg ctaaacttct gcttgtttct gatattgctt
      121 gtttttctcc aatgatccgc acttctgttt ctaaagacac atcaaatttt tctttgatta
      181 cttcttggat gtgcgcaatc aattcaatat aatctgtcgc tgtcgcatga tcgatattga
      241 cgatgaatcc agcatgtttt tcagagactt gtgcaccgcc ccatttcagg ccttgcaatc
      301 ctgcttgttg gatcagctga cctgtatagt gtcctactgg gcgcttaaag acacttccgc
      361 atgaagggta ttctaatggc tgttttgatt gacgcaattc ggtcaactca tccattcttg
      421 ctttgattgc ttcataatcc cctgtttgca aactgaatcg agcttccaat acgatagcac
      481 gtaattcttg gactttactg tgacgataag aaaataccat ttcgtctttt gtcaaagttt
      541 tcaatgttcc atcttcaagc aaaagatcca cttcagcaaa aacatctttg atctctccgc
      601 catacgcacc tgcattcata taaaccgctc caccgacact tcccgggatg ccgcaagcaa
      661 attcaaatcc agtcaatgat tctgccaaag ctgcataagt cgtatcaatc agtgaggcac
      721 ctgcttccgc aatgaccatc gttcctttga catgaatctg tttcatttca gtcagcatga
      781 tgactacacc gcgaatcccg ccatcttgaa cgatcagatt gcttgcattt cctaatacca
      841 tccaaggtat atcatggatt cggcaataat cgatgatttg ctttacttct tcttgttttt
      901 tcgggaatgc aaggacatca gctggtccac ctgttttcgt aaacgtatag ttcatcaatg
      961 gttcatcaaa caatatagta atcgtggcta agctttcaac tatttcttct ttattcactg
     1021 aggattttcc tttcttttct caaacgtcct cattattcta tcacgcttaa tacaatcttg
     1081 ccagtcttgt tcaaagttga tgacggtagt gatctttaag cacttcctca cataattcat
     1141 gacttcttac aacttctttt tgtttaagca attctgtttc cgatttacct aaacggatct
     1201 gagaaaagaa ggcctctacc attggctcaa acccgcgttt gactagcgtg tttgtccagt
     1261 cgccaaattc tttcactttt tttccatcag catcttgaat gacaagctgg gtcagatcgt
     1321 ttacttcata agtaccattt ttacttgtta cttgatattt ttctatatta gcgccacttg
     1381 ctaaatccat cgttaaaata gccgtttgat tttgtgtttc taattgtaaa atggcatatt
     1441 ccaaaaattc ctttgtttct cttatctggt attttgttct caaaatgggt tcatctaata
     1501 aatacacagc tgtatcaata agatgtaaaa acaaatcata aaggacgaac tctgtcgttt
     1561 cttttgccgc tattcgattt ttttgcaggt ggatcaaccg cttatctggg atacgtttca
     1621 attcttctac cataggagca aagcgccggt tgaaaccgat catcaaaatt atttggttct
     1681 tatcagctaa ttcttgcaat tcacgaactt catttagatg gacactcagt ggtttgtcaa
     1741 tataaacatg gataccattt tctagacatt ttttggctaa ttcataatgt acttttgttg
     1801 ctgcgtggat catacatgct tcgatttttt cttctaaaag ctcatcgatc gtttcatata
     1861 gatgttggaa cccgtactct ttttgaattc tttcttttgt ttctttattt cttgttgcaa
     1921 aataaaaatc aagtgttcct tgttttttcg cataagttgg taaatatgct ttttctgcga
     1981 tattccctac accgattaca ccaatcttca tctactttcc tcctggatca tttagttact
     2041 ttcatcttac accatttttt actaaaatga ggtacaatag tatcgcaaag gtaaggaaat
     2101 taattcgaag gagtgtgctt ttattgaaat ttatctcttg gaacgtaaac gggcttcgtg
     2161 ccatcgtaaa taaaaatttt ttagaagttt ttcatgagtt agacgccgat tttttctgtc
     2221 tgcaggaaac gaaactccaa gcaggacaaa ttgatttaga tcttccaggt tattatcaat
     2281 attggaatta cgcggaacga aaaggctatt ccggcacagc gatttttgct aaaaaaccag
     2341 cattgaatgc cacttacgga atgggtatcg atatccatga tacagaaggc cgtctgatta
     2401 ctttggaata ttccgatttc tttcttgtaa cctgttacac gccaaattct caaagcgaat
     2461 taaaacgttt agattaccga ttagaatggg aagaagcctt ttataattat ttggaaaatc
     2521 taaaaaaaca aaaaccagtc atcgtgtgtg gggatctaaa tgtcgctcat caaaagattg
     2581 atttgaaaaa ctggaaaacg aatcaaaaaa atgctggatt cactccagaa gaaagagcgg
     2641 ctctttctcg actgttggat aacgggttta tcgatacatt ccgttatttt tatccaaccc
     2701 aggaaggtgt ctactcttgg tggaactacc gattcaattc aaggaaaaac aatgctggtt
     2761 ggcggatcga ttactttttg acgagcaaag atttagagcc aggactagct gatgcaaaga
     2821 tccatacgga cattatgggc agtgatcatt gtccagtaga attagatttg aaataaagag
     2881 gttgtgaaaa agtcgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
     2941 cattttgcat aaatttggca taataccgag aaactggctt ttgaacactg tttatcgaga
     3001 gattgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
     3061 atatttttcg tttggttggg cttattaccg cagatacaag cttttgaaca ccgtttatta
     3121 agaggttgtg aaaaagctgc actgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3181 ctcatatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta
     3241 ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag
     3301 ttcctcatat ttttcgtttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt
     3361 ttattgagaa gttgtgaaat cagcgtcctg acctgagtat tagagaaaca aatggcaaaa
     3421 tagttcctca tattttgcta ttttttgagc taatacggag ggtcagctga ttgaacactg
     3481 tttagtggag aggttgtgaa atcagcgtcc tgacctgagt attagagaaa caaatggcaa
     3541 aatagttcct catattttgc tattttttga gctaataccg agggtcagct gattgaacac
     3601 cgtttagtgg agaagttgtg aaaaagtcgc ctactttcga gtcgtaagaa atttttttga
     3661 aaatggttcc tcacaacttt gcataaattt gacttaatac cgagaagtca tgatccccat
     3721 ctaactccaa atcacaagga atgagttcaa aaactagctt ttctcaacaa atattacata
     3781 aaaaattcta tgttttttgt taaatttatt tgcttttttc aaaagtatgt tacagtaatt
     3841 tcccaaggag gttttacaga aatgaatttt gttgccatgg attttgaaac tgccaatcat
     3901 cagccttata gtgcttgttc tcttgcgcta gtcatggtaa aaaatagcca gatcgttgat
     3961 gaattctata cattgataca gcctgagact ccttttttct ggcgaaatgt ccaaattcac
     4021 ggaatccatc aagaagacgt acgcaatgcg ccgaaattcc cagaagtctg gcaaactatc
     4081 caaccatatt ttcaaaaaaa tcgtttagtc gttgcacata atgctgcttt tgatacaaag
     4141 gtcttagcgg gatgcttaga ttattatcat cttgaacagc ctaattatct ttctttatgt
     4201 acagtgaaaa gtagccgtcg cctgtttcct gaatttccaa atcatcgatt gaacactgtg
     4261 tgcgaaaatt tgaacatcca attagatcat caccatgatg cattggaaga tagtcgtgca
     4321 tgtgcggaaa tcctgcttta tcaagaaaaa aaattcggga cagatccatt gaaaaaactt
     4381 gtgactataa agtaaaagaa tacttatgaa acacacaaag aggtcgtgag cctgccgttt
     4441 cactccgagt cacaaggagc aatttcaaaa aatagcttct catgtttatt aaatattgtg
     4501 actggtgacc gaggagctgg caggagaaca ccgtttattg gagaggttgt gaaaaagctg
     4561 ttctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt ttcatttggt
     4621 tggacttatt accgcagatg caagcttttg gacaccgttt acc
//
LOCUS       DAGCYL010000184         4626 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.187, whole genome shotgun sequence.
ACCESSION   DAGCYL010000184 DAGCYL010000000
VERSION     DAGCYL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.187"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(44..1276)
                     /locus_tag="MB216_002467"
     CDS             complement(44..1276)
                     /locus_tag="MB216_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBT4527250.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1530..2099
                     /locus_tag="MB216_002468"
     CDS             1530..2099
                     /locus_tag="MB216_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4527251.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2277..2717)
                     /locus_tag="MB216_002469"
     CDS             complement(2277..2717)
                     /locus_tag="MB216_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBT4527252.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2875..3639
                     /gene="map"
                     /locus_tag="MB216_002470"
     CDS             2875..3639
                     /gene="map"
                     /locus_tag="MB216_002470"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBT4527253.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3671..4594
                     /locus_tag="MB216_002471"
     CDS             3671..4594
                     /locus_tag="MB216_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBT4527254.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT         1397 a          859 c          912 g         1458 t
ORIGIN      
        1 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
       61 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      121 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      181 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      241 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      301 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      361 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      421 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      481 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      541 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      601 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      661 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      721 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      781 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      841 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      901 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
      961 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1021 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1081 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1141 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1201 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1261 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1321 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1381 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1441 aaaactaatt ttgcatatca aaaaaacatg ttacaatgaa attaatcaat ggtggaagcg
     1501 ttataaaaaa attaaggaga ttaaaaaata tggcaagaaa aaagacgatt actagagaac
     1561 agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga tttactgcga
     1621 gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag tttaaaaata
     1681 tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa tatgaagtgt
     1741 tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaattat atccattttg
     1801 caagaagaga gaagaagttg tatcgttcac tgtacttaga agaatatggt ggaggaaaag
     1861 agatgcagaa tttctcttat acttactttg tgaatacagt gaaacgcgat ccagcttatg
     1921 tgaatctttc agaagagcag atcgaatctt tgtataatgg gacatggatc atagctactg
     1981 ggatcgcagc tttgatgtct tcaagcatca tccatccaac tgatgaacag attgaaaaga
     2041 tgatccaaga cagtatagat gctatcttga aattcaaaga accaatcgaa atcgattgaa
     2101 tatactcaaa agttccacaa atcagtaggt aacaagagat gtgtcaaaat aaggaaaagt
     2161 tcgtttttct tacagcgtat gcatttttaa ctcccgaaga actcttccat gaatgcaatg
     2221 aataaaagag ccatacagat tgaataaatc tggatggctc ttttattgca agtatattaa
     2281 atttttgcga ctagttcttt agcgaaagct tctaaatttt cgatatcttc ttcttcagca
     2341 ttcagatcca ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc aaaagcagca
     2401 tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag tatcaccaga tccgcagaca
     2461 ccgtaaattt ttcccaatag atccaattct tgcaagtctt cataaaagtc tacgatctca
     2521 tcaggcagat cgccatcatc gtatgtataa gtagctacta cgcaaatatc tgcctcctcg
     2581 aattcatctg catctacttg ggtacattcg ttgatttcga cttcgatatc cagattttca
     2641 aatgcttctg caacaatatc ggcgatttct tcagtgtttc ctgtcatgct agcatatacg
     2701 atttttgcta aagccatgat gtgcctcctc gtttataatt atttaacaat tatgaattat
     2761 accaaaacat cattgaaaag aaaataaaac ctaaagacac ctggggaatg tcatttggct
     2821 aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa aaggagacga aaatatgatt
     2881 actttaaaat cagcacgtga aattgaaatg atggcagaat ctggagcttt attagcagat
     2941 gtacataaaa atctgcgtga ttttataaaa ccaggaatca caagttggga tattgaggta
     3001 tttgtcagaa actatatcga gagtcatggc ggaattgctg cgcaaattgg ctttgaaggg
     3061 tatgaatatg cgacttgtat cagtataaat gatgaaattt gtcatggatt ccctcgtaaa
     3121 aagcctttga aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt gaaaggcggc
     3181 atttctgatt cttgttgggc ctatgtagtt ggagaatcta cacctgagat cgaccgattg
     3241 atggaagtaa cgaagaaagc tttgtacatc ggtattgagc aagcacaagt agggaatcgg
     3301 attggtgaca tcggtcatgc tatccaaact tatgtagaag gcgaaaatct agcgatcgtt
     3361 cgtgatttta ttggtcatgg cgttggtcct acgattcatg aagaaccagc agttcctcat
     3421 tacggagaag ctggaaaagg gttgcggttg aaagaaggca tggttatcac gatcgaacct
     3481 atggttaata ccggaacttg gaaaatgaaa atggatccta acggctggac tgcgtataca
     3541 agagacggcg gactttcttg ccaatatgaa catacattgg ctattactaa agatggtcca
     3601 agaattttga catcacaagg agaagaaggc acttattgat ctatccagtc aataggtacg
     3661 ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga tgcgctttat
     3721 tgagacgact caaaagcgca tcactgattc agaaatgagt acgacctctg tcgtcgttgc
     3781 ttattattta ctcttgtctt tatttccatt gattattgct tttggtaata ttttaccgtt
     3841 tctccacatc gatcaagaga ctgtactgac ctatttacga gaagtgattc ctgagaccgt
     3901 ttaccagttt atcggtcctg caatcaaaga ccttttgaca caaagttcag gaggcttgtt
     3961 atccgtttct gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg ctttgcaaat
     4021 tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg tccgtttctt
     4081 ctcactaatt gtcattgtct tgttcatgat tgctattagt ggggtaacta tcgttcttgg
     4141 gttgggacag ctgattttgg atgctattca gccaatcgta caaatacccg tgaactttat
     4201 cgatcagttc caaactttga agtggccaat cacattggta gctttatttg tcatcatgtt
     4261 cttgatttat ttaattgtcc ctaatgctca gttgaagttg aagacgatca ttccgggcac
     4321 gatcttcgct actgtcggct ggatgctgtt atcacaagtg tttagtatct atgctaggta
     4381 ttttgcaacg aggatcagcg gttatcagat cataggaagt tttattgtac tcatgctttg
     4441 gcttaatttt gctgctacga tcatcatttt gggcggaatc atcaatgcag ttgttcaaga
     4501 atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat ggttcaataa
     4561 attgaaaact aaattttctc ataaaaaaaa gtagaaacaa gagttcttat tgtaaattta
     4621 tatcag
//
LOCUS       DAGCYL010000185         4511 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.188, whole genome shotgun sequence.
ACCESSION   DAGCYL010000185 DAGCYL010000000
VERSION     DAGCYL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.188"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            168..1049
                     /locus_tag="MB216_002472"
     CDS             168..1049
                     /locus_tag="MB216_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4527255.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1062..2672
                     /locus_tag="MB216_002473"
     CDS             1062..2672
                     /locus_tag="MB216_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBT4527256.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2749..3408)
                     /locus_tag="MB216_002474"
     CDS             complement(2749..3408)
                     /locus_tag="MB216_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBT4527257.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3691..4356)
                     /locus_tag="MB216_002475"
     CDS             complement(3691..4356)
                     /locus_tag="MB216_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBT4527258.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1227 a          869 c          882 g         1533 t
ORIGIN      
        1 gtatcaagct agcaattcct tgtttatgaa ttcattgaca gaaatttata aaaagaatat
       61 tatgtactaa aataataaaa aaagttttat aaaaaggtat aaaacctttt tattaaacta
      121 tttaatcttg tttagtatat atttttagaa aaaggagtgg ggtttttatg gcagaaatcg
      181 tcaaagtcac acatttgaaa aagagctttg gtcgtttcga agctttaaaa gatgtctctt
      241 tcaccgtaca ttcaggtgaa gtcgttggat ttatcggacc aaatggttct ggaaaatcca
      301 cgacgattcg gacactttta gggattataa aaaaagacgg cggaacggca acgatttttg
      361 ggcaagatgt atgggaagat agtttatcta tccatgaacg tctttcttat gttccaggcg
      421 atatctcttt gtggggaaat ttgactggag gagcgatcat cgatctcttc atgaagcttc
      481 atggaaaagg gagtgctcaa aaaaaagatg aactgattca acgtttctct cttgatccta
      541 agaaaaaagc aaaaaactat tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt
      601 tggctgttga ttcggatctc tatttatttg atgagcctac ttctggttta gatcctttga
      661 tggaaacgat tttccaagaa gaagttgaaa aattgaaaga acagggcaaa tccatcctac
      721 tttcttctca tattctcagt gaagtcgagc gactagcgga taaagtcgtg atcatacgcg
      781 aaggaacact cgatgaagaa ggaacactcg atgaattgcg tcatttgact cgttcgacca
      841 tcactataga aacagaagca gagaatagtc acttagcaca attgaacggt gtccatgact
      901 ttgtgcaaaa aggtcacctc gctcaatttg ctgctgataa cgaagctttc gatgtgattt
      961 tagctgaagc gtccaaatta ggtgtgaaaa aatttgaatc tattccaccg actcttgagg
     1021 atttattcat gagacattat gaaggatgag gaggaagcga aatgactgat aaattttcac
     1081 gctggtccac ccttttttct caaaacctta gccgtgactg gaaaaagatc ctgatatggc
     1141 tagtcggtgt cactgctttt tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg
     1201 gacaaggatt agctggatta tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg
     1261 gaccaactcc agcttcgaat gctgctgagt atactttagg agcgatgtat gcaaatgaaa
     1321 tgttgctttt ttgcagtttg tttgctgctg tgatctctgt tttacacgtg gtgagccaaa
     1381 cgagaaaaaa agaagaactt ggtctgattg aacttattcg ttcctttcga gtcggacgtc
     1441 aagcaaactc tttagctgtc atcgctgaaa gtattctgat caatttgctt ttagcaatat
     1501 cgatcagctg tacgatgatt tttttccaag cggattcgat cactgcagaa ggtgcttggc
     1561 tttttgggct atctgtaggt agtatgggga ttttggggag tgccgttgca ctgtttgctg
     1621 cacaactgat gccaagttca gccagttcga atggtctgtc tttagcgttt ttaggtggac
     1681 tttaccttct acgagcaata acagatgtag ctgattcctc cctttcgatg tggaatcctt
     1741 taggctggtc ttatttgacc tatccattta caactaacaa ctggattcct ttgctttatt
     1801 tgtttttgtt cagtctaatc ttgataatca gttcatttgt tttagaggaa catcgagatt
     1861 tgaacaccgg ctatatcccc gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat
     1921 ccttgcgtgg tttattattc tatctaaata aagggatgac aatcagttgg cttatcactt
     1981 ttggtttgat gggggcggca tacggctcta tctacgggaa tatgcaacag tttttagaaa
     2041 gtaacgactt aatgaaacag atgtttatcc aatctggcgt ctcaattgaa aaaaacttta
     2101 caagcacgat catggtcgtc ttgatttgtc tagctgctat tttaccaatt gctagcatca
     2161 atcgtttatt tacagaagaa acccacggac gtatgaatca gctgttcgca acaaaaattt
     2221 ctaggaacca actttattgg accaatatgt tactagctat tctatttgct atattggcaa
     2281 tcctgattag ttctggtgca ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag
     2341 acatggatct attgatgttt cttaaagccg ggttcaattt cctgcctgcc gttttagtgt
     2401 ttatcggaat tagtggcttt ctattcggat ggcttcctcg aatcggtaaa cttgcttata
     2461 tctacttagc ttactccttt gcactcaatt actttggcgg aattttggat ttaccagaat
     2521 ggttttccaa aacagctgtc cctagctggg ttccacggat gccaaacgat tcttttgatc
     2581 tttccgtgtt ccttacgtta tcatgtatca gccttgcact gttcgtcctt ggttctattg
     2641 gttataggcg aagagactta ctagaaggat aataaaaaag catgtacaat ttcatgctag
     2701 cctccaaatc tgggtcaaaa atccaacatt tggaggtcat ttttttattt acaagtcttc
     2761 taactgctct ttatcaatga tccagacttc tcgattcttt ctttttacat aaccattctc
     2821 ctctaataat ttaaattttc tagataaagt ttcgggggtc gtaccgataa atgcagcaag
     2881 ttctttcatt ttcattggca ggaaaaagtg atccttccct gtcgctttag ctaagtcaag
     2941 cagatagaca gcgactcgtt cttctacttt ttccatcatc aaaaaagttg tctgctgctg
     3001 tacttttgtt aatttctcaa cgttgattgc taaaagtttc agactaaggg cgggataatg
     3061 cagtaagagt tccgagaaat ctttttgtct caatatgcaa atttccgttt tttctagtgc
     3121 ttcgccaaat aaagactcat ttggaacgcc tagcaactgg ctctctccct catatccgcc
     3181 cggctctatt actcgtaaaa gctgttcctt tccgctagca gataattgat acactttcat
     3241 actgccggag gccacgatca ctaattgagg ttctccattc ggcgcaagaa tctgttctcc
     3301 tttttccaca tagtgatgtt tgacaagtgc gtgtactttt tcttgatctt gctgtactaa
     3361 gtgattgaac aaaggaacaa atgaaacaca taaatgatca tgactcataa tctctcctcc
     3421 ttctaaacag ctatttctaa taaagaaaca taacttccct tattccaact gataattttc
     3481 ttttatcata gcataaacgt caagcgaacc agataccagc aaatagagtg gatttctttt
     3541 tgtcccttgc tttattttcc ttcttttgag accttaagta attgattcct atcaattttt
     3601 taaatattta ctttttgaat aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt
     3661 ttgtttttcg cattgcttat ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg
     3721 atatagtggt gctagttctt gtaaggtttc agcggttatt tgctcaacgt gttcgatttc
     3781 ttgcggatag aagaatagtt tacccatttg aaaggttcgg tctctcatcg cttttttcaa
     3841 ctcttcttgt ttctttgtat cggacaaata gtcagacaaa gctaagtggt cgtcaagttc
     3901 atgttctggt tgcggccata tgtaataatc cttgatatcg actgttttgc tccactcttt
     3961 cagtatcgca agcaactggt tatacgctgc aatgctacca tatcgttctt cgcttttata
     4021 atgttggtac atcaaataga tttggtattg tttttgattc aacaaaactg ctagacaggc
     4081 attctcgtct tgacgagatt cgcttcggta cgcgctccaa aaatgactgc gtaaattcca
     4141 gccattcgtc cagctttcga ttttaggttt gctcatgccg tacatatcag gcaaaagtgc
     4201 agctgtctgg agctgaatct ctttccattt ttgccagtgt tctttatatt ctttcttgat
     4261 ttgttcaatc ttatctgttg gatggctctc tttgagctgt ttgaatgcgt agattggtcg
     4321 atcaaacaac tgataatctg attttccaag ttccattgtt tcgcctcttt ccgttatctt
     4381 ttgtattcta cctgaatttt gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc
     4441 atcaagtcat aagaacagcc aaacaaaaaa ctgcgatgag catcaccgca gttttattgg
     4501 ctctataatt t
//
LOCUS       DAGCYL010000186         4464 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.189, whole genome shotgun sequence.
ACCESSION   DAGCYL010000186 DAGCYL010000000
VERSION     DAGCYL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.189"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            573..1007
                     /locus_tag="MB216_002476"
     CDS             573..1007
                     /locus_tag="MB216_002476"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527259.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2297..3220
                     /locus_tag="MB216_002477"
     CDS             2297..3220
                     /locus_tag="MB216_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4527260.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3236..3775
                     /locus_tag="MB216_002478"
     CDS             3236..3775
                     /locus_tag="MB216_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBT4527261.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1667 a          667 c          756 g         1374 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcacgcgctt
      241 atgaagcgct accaaaaatt taccatattt tacaaaaaat aacaagtctt aaaaaaatga
      301 cagataaaaa tgcacaaaga attagatata gataaccgcg aaaaatcaag ctttagaaag
      361 ttgcttttag ctagaccaat taaaaatcac aaagtgagcg tctctatcca ttggtagagg
      421 ggctacttgg catagtgtac attatgtcta ctaaccttta aaataacacg ttttataaaa
      481 taaaccctct tgacaatatt taattttctg attattctaa atatatacag aaagcgcttt
      541 cctaggattt tatatctaag gaggaagtaa caatgaaaaa taaaattatt aaaattatta
      601 cttgtctatt tttgttcggt atgattgttg gagcaagtac tccgttaaat ctgtttcctt
      661 ttagtgctag aggtataaaa gtggaagctg caagttcacg atataatcat aatcatcgtg
      721 gatttacttg ctataaaatg aactattatg ttactaataa aatgtgtaga gatttgaaaa
      781 aaaattataa taagctaaag actcctgctc aaattgcatc ttttattcct attggtggag
      841 ttggaacatg ggttattaca aatgctttta gtagtgctgt tgatgcaatg aatgtctttg
      901 taagagctgc aaatcaagga aaaggtgtac aattaacata taatgctcac tttagcaata
      961 caacatcata tcaatacaat gattacgctc gttatgtaat aaaataatca gaaagtgaaa
     1021 ctatctcatg aataaaaaga ttttagctgt aattatttca ataatagttt taatcactat
     1081 gttcttcata tttaggctaa ttttcaatat aagcttacaa aaaagtattc tttatctaat
     1141 tcctatagca tttatcttag caatttttag aagtatttac ggaaataaaa actctaaata
     1201 gctaaaaata aatcatacta aattttacaa aaagtggcta gcattttcta gccacttttt
     1261 gtattgtgga gcgtgggttt tgagggggat tcaaggggga gcttgcgccc ctttgacaag
     1321 cggttatttg caccaaatac ggaggtattt ggctagcaaa tatagagctt gccaaaccct
     1381 tttagtgagt tttgtagaaa gtacctttaa aggtactttc acattacgaa cgatacgtcc
     1441 gtaggacgta ctgaaagggt gggacttatg agcaaattaa tgcaaaattt ggctagcaaa
     1501 aaagctaaaa aatatatgct aataatcgag cgaaataaac actttttcaa gtgagcgaat
     1561 ccgccatgac aaagctgaag cagtttattt cagctttgtc atggcgtgtg ccgacttttt
     1621 taaaagtcta ggcacacgag caaatatctt tatcaagctc taatttgcgt tttaagacgt
     1681 ttgaaacgcc taagtatata aatatataag aataggttta agagtgctta aatcgaattt
     1741 tagagggcat ttagacaatt ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgt
     1801 tttaagaaca agataaggga tttatactag gactgtctta aaatgtctta aacgaacgct
     1861 cagagcttct caaagggttc cttatgaacg gcttttagca aaggagccgg aggcgactgc
     1921 gcagtataat gtgagagcag agcgatcaca cctttttagc gaaccgtagg gagcaaggtt
     1981 tggaattggc agttttgata aattcgcgaa cctgtttaga gaaaaaagga gtgatttttt
     2041 aggcgggaaa attagcctcc cctcttttta tatctctttt tatatctctt tttatatctc
     2101 ttttatgtcg gctgtgtcct tactctccca agcaattcag agttttttca agtaggtttt
     2161 ctacgctttt caagtaggtt ttctacgctt ttcaagtagg ttttctacgc ttttcaagta
     2221 ggttttccga cattattgag tatttttata aagtatgtta cttttatttc atactttata
     2281 aaagtaggag aagcttatgg caaatgaaat tgtaaaatat gaaaatagat taaatcatat
     2341 tcctctgaga aaatttaata gtcgtgaaat gaacttattt ttttcaattg cttcacgtgt
     2401 acgtgaaaaa ggtgtagatg agattatttt ttcatttcac gatttgaaaa aactaagcgg
     2461 ttatacagaa cgtggggaac aatttataaa agatcttagt agcacgtatg acaaattatt
     2521 aagtattaat gcttggaatg atgatggaaa tactttaact aaatttgttg ctttcacaaa
     2581 atatagcatt atacgagata aacaacttgt tataatttca gttaatccag aatttaaagg
     2641 actttttaat caattaaaga actggacaag atttaatcta gagcaattta ctaatttgga
     2701 tagcacatat tcaaaaacta tgtttcgatt attaaaacaa tatcgaactc aaggctggac
     2761 tgaattttcc aaagaaaaat tttttgattt actagatatt cctaaaagtt attggaatag
     2821 tccgtcaaat attgacaaat ttatcttaag gccaattaaa gaagaactca ctcccttatt
     2881 taaaggatta actattcgta aaaaatatgg taaaggtcga ggtaaacctg ttatcggtta
     2941 tcgttttact tggaaagctg aaattaacca tgctgacgat ttttccaaag ggaaacaaga
     3001 agatttacgt ataaaattat ttaatattga tcacaatgga gaattaacac gagaagaaaa
     3061 atggcgcgct aaagatcgaa tactaaatct tcccttaggc actcatgaag ctgattttaa
     3121 acaacaacaa caaactgaag aaaaacaggc agaaaaacga gaactttcaa acaaactcaa
     3181 acaagattta ctaggaaatt tacaaaactt attcgattaa gcttaaggag tatttatggc
     3241 agataaaaca atcaaagaac ttgcggaaga attaggagtt agtaaaacag caattaacaa
     3301 aaaagtttct gacaaaaatc gaaaactttg gtttgcaaaa aacggaaaca agtttgtgat
     3361 taacgaaacc ggtcaaaaag ctataaaatc aatgttttta gtcgataacg caaacttaaa
     3421 tcgaaaacta gttggcgaaa aatcaaaaac caaaaaccat aaaaacacca accaagtttc
     3481 cgaattaaaa ctcctttctt tcctagaaaa acagttaaat cgaaaagata tacagctaca
     3541 agaaaaagat aatcaaataa aacaaatgca aaaattactc gaccagcaac aacaactcac
     3601 tctacaagcg aaccaacaaa ttgagaacct acaagaacag ctccatttaa cttatacaga
     3661 agaaactcca acaaccgaat caactgcctt ttctgaaaaa aaagcagaca atacacaacc
     3721 acttattgaa aaaaagtggt ggcaaatttg gaaatctaat agaaccaatc tctaaaattt
     3781 tctagaagtt agttcggata gtagaggaaa aagttgtact acctgatggg gagatcactc
     3841 aaatttttat gagaaagaaa aaatagttta caactgtaaa caatcaatgt ttatagttgt
     3901 aaactatcaa actatatggt gacttattgc ttaaaatagg ttgaaatctc actcaatttt
     3961 tggtggaaaa acaaaataaa ttctaatcga ttttgttgaa aaagtcataa aaaaacaaat
     4021 gtgattttaa caaaatttca aaaaaatcgt ttgaaatttc gtttattttt tttgagaatt
     4081 ttgatcatcg tagggtcatt gtagaatcat tatgagatca tcgtgggatc atcgaataaa
     4141 gtatgggaaa ggcttgcggt gcttggtatt aagggcattc tccgcctccc aggttcttga
     4201 ccattttctc tcaacgtggt aaaaatccca cttataagag aaaatggtca agaacctaaa
     4261 cggtcggcga agaaaacata ccaagcacca caagcaaaaa aagttagagt aacttccaaa
     4321 aaagaaaaat tttttagaac aacttttgaa aatttaattt tgatttatat ccgttcaatt
     4381 taaacatttg ataaactgaa ggctagtatt taaaatgtgt atacaagtgg tagcgttaaa
     4441 aaaagtggag gtataaccta tgtt
//
LOCUS       DAGCYL010000187         4413 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.190, whole genome shotgun sequence.
ACCESSION   DAGCYL010000187 DAGCYL010000000
VERSION     DAGCYL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.190"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            427..984
                     /locus_tag="MB216_002479"
                     /pseudo
     CDS             427..984
                     /locus_tag="MB216_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1042..3081
                     /locus_tag="MB216_002480"
     CDS             1042..3081
                     /locus_tag="MB216_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBT4527262.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3190..4341)
                     /locus_tag="MB216_002481"
     CDS             complement(3190..4341)
                     /locus_tag="MB216_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBT4527263.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1594 a          808 c          862 g         1149 t
ORIGIN      
        1 tgttattttt ttctaaaaaa tattaaagat tgttaaattt cactatttca tttcaaattc
       61 cattgcctaa tcattaggaa aagtctatca tgaactcgta aatagaatat agtaataacc
      121 ttgttaggtg aggctcctat acaaacatag gctgttgccc agaaacgtcg aaagtcgcca
      181 atgggtaaaa cagaaactgt tggattaagg ctttttccaa tagctaagaa tgttttcttt
      241 acgttgtata gtgctaaaac tcggacgatg agatgatgct aggtcagaat tcttgttcga
      301 aaaggtttct gaccttttct ttatattttt gtgactgact tatttgatga gacaaactcg
      361 ctcctcacct gacaagtgga ttgctgtgtg aggaggagat gcggtgaaaa aagatcgatt
      421 ggtggaggtc gctcggattc acttctggtt gcacaataca accagaaaac acaacaagca
      481 aaactgatct ctatcatgag agattcatat gtcgatattc caggttacgg aatgaataag
      541 ataaatgcag cttactctta tgggggagtt gatttattaa atcaaacatt aaaggaaaat
      601 ttcaaatttg aagctcccta ttatgcaagt atcacatttc aagattttat cgattgcgtt
      661 aatgaactgt ttcctggtgg agtcaagatc gatgcagaaa aatctctaga tttagatggc
      721 gtatatataa acaaaggaga gcaagtgatg gatggtaata ctttactcca gtatgctcga
      781 tttcgggaag acaaagaagg agacttcggg aggatcagaa ggcagcagca agtgatcaaa
      841 gctatctctc agcagctgaa agacgtaact tcaatattca agctacccaa agcggttggg
      901 aaattacttg gtagtatcca aacgaacctt ccagaaagtg tgcttctgga ttgtggtatg
      961 gatttcctca aagacatatg ttagaataaa caggtataaa tagtgaaata aaggaatgac
     1021 aagcaagaga aggaggaaaa aatgaaaaga agtgacaagc acggcaaaaa tcgaacaggc
     1081 gcttatattg ccggcgcagt gatcttaata gcagctgcgg gtggcggtta tttttactac
     1141 cagcactatc aagaaaccca agcagtagaa gctggagaaa agacagttga gcaatttgtc
     1201 caagctttga acaagggaga ttataacaaa gctgcagaaa tgacatcaaa aaaggcggca
     1261 aataaaagtg cattatctga aaaagagatc ttagataaat accagaatat atacggtgct
     1321 gctgatgtca aaggacttca gatatcgaat ctaaaagtag ataaaaaaga cgattctact
     1381 tacagttttt catataaagc aaagatgaat acctcattag gtgaattgaa agatctttct
     1441 tataaaggaa cattagacag aaatgatggt caaaccacga tcaactggca acccaacttg
     1501 gtttttccag aaatggaagg aaatgacaaa gtaagcctga ccacacaaga agcagcaaga
     1561 gggaatatta tagatcgaaa tggtgaacca ttagcaacaa caggcaaact aaaacaatta
     1621 ggagtcgttc caagtaaact tggagatgga ggcgaaaaaa cagccaatat caaagcgatt
     1681 gcttcctcct tcgacttaac agaagatgct atcaatcagg cgatttcaca aagctgggta
     1741 caacccgatt actttgtccc attgaaaatc attgatggag caacgccaga acttccagct
     1801 ggagctacca ttcaagaagt agacggcaga tattatcctt tgggtgaagc agctgctcag
     1861 ctgattggtt acgtgggaga tatcacagca gaagatattg ataagaatcc agaattaagc
     1921 agtaatggaa aaatcggtcg ctcaggtttg gagatggctt ttgataagga tcttcgtggg
     1981 acaacaggtg ggaaattaag cattacagat gcagacggtg tagagaaaaa agttctgatc
     2041 gaacatgaag ttcaaaatgg caaagatatc aaattgacaa tcgatgcgaa ggcacaaaaa
     2101 atagcttttg acagtctagg aggaaaagct gcatcaactg ttgcgacaac gccaaaaacc
     2161 ggtgatcttc ttgcgcttgc tagctctcca agctatgatc caaacaaaat gacaaacggg
     2221 atctcacaag aagattacaa agcttatgaa gaaaatcctg aacatccatt catcagccga
     2281 tttgcgacag gttatgctcc tggatctacg tttaaaatga ttacagcagc aatcggtctc
     2341 gacaacggca ctatcgatcc aaatgaagtg ttgacgatca acgggcttaa atggcaaaaa
     2401 gacagttctt ggggatcttt taaaacaacg cgtgtcagta gtgatgtatc acaagtagac
     2461 ttaaaaactg ctttgatcta ttccgataat atatatgcgg cacaagaaac gttgaaaatg
     2521 ggtgagaaaa aatttcgtac aggcttagat aaattcattt ttggtgaaga ccttgatttg
     2581 ccaattagta tgaatccagc acaaatttct aatgaagata gctttaactc agatatcttg
     2641 ctagctgata ctggatatgg acagggcgaa cttctaatta ctcctatcca gcaagcagca
     2701 atgtattctg tttttaccaa caatggcaca cttgtctatc ctaaattgat tgcagataaa
     2761 gagacaaaag ataagaagaa tgtcatcagc gaaacagcag tacaaacgat cgtgccagat
     2821 ctgagagaag ttgtgcaaga tgtaaatggt acagcacatt ctctttctgc tttagggatt
     2881 ccgttggcag cgaaaactgg tacagcggaa atcaaagaaa aacaggatgt aaaggggcaa
     2941 gagaacagtt tcttgtttgc tttcaatctt gataaccaag gttatatgat ggtcagcatg
     3001 ttggaaaata aagaagatga tgattcagca accaaacgag catcggaact attgcaatac
     3061 ctcaaccaaa attatcaata aaaaattgct ttttaagtac agtaaaagga ctgtggcgtt
     3121 atcgtcacag tccttttttc aaataaacgg ttttcacgat gtctctttca caacttcttt
     3181 ttgatttact cacacttcat aaactttttt tgtaaaatat ttgagaacaa gtggagaaat
     3241 aagtgtactt aataaaatca cgataactaa aggtgaatag tactgtggtt cgatcaattg
     3301 actttgctgt ccaatttgta aaatgatcaa agccatttct ccacgcgaaa tcataccagc
     3361 accgaccatc aatgaactat gttgagaaaa accggcaatc tgagcaccaa aataacctcc
     3421 aagcaatttg gtcaaaatcg ctacaagcgt caatatagaa ataaagagta gttgttcact
     3481 aaagtcagaa aaatcaactt ctaatcctac actgacaaaa aatactggta taaatactgc
     3541 atatcctaaa gcctctacat tgtggtatac ttcttcttta acctttgtct gactgacagc
     3601 tatcccagca aaaaaagcac caatgacaga gctaagccca acaagatccg ctacgtaaga
     3661 cattcctaaa caaataacta gcgatacgat gataatagaa gaatttgcgt acactttttc
     3721 tgcaaatgaa agtaggaaag gcgcaatcca tttgactaat aagaaaataa aaacaaaata
     3781 aattagctgt tcaatcaaaa caagcggtaa agaagcattg ctagaagctt cccccgttag
     3841 aaaagatagg ctgaaactta gaactaaaac gactaaaatg tcatctacca cagaagcacc
     3901 caaaattgtt gatccttctt ttgtattcac tacgtttagt tcttttaata cttctactga
     3961 aatactgaca gaagttgccg caagaataat cccaaagaaa atcgcttcat ttcccgaaac
     4021 ttggaatgct ttgcctgtca accagccaaa aacaaccggg aagatgatcc caagcaaagc
     4081 aacgaacata ccaggtcgga aatatttttt caaaagagat aaatcacttt ccagaccagc
     4141 taaaaacatt aataagatca caccaatctc cgaaaagtca tggactagaa tatcaggttt
     4201 gacccaattc aatccagctt ggcctagcaa aacaccaaca agtaattgtc caatcactgc
     4261 tgggatacct aatctccgcg agatatgtga gccaatcgtt gttgaaatca atatcaggca
     4321 taaaatacca ataaattcca ttctcatcat tcctttgtat aaaatatagc ccgaacttag
     4381 cataacatac attattgaaa aaataggaaa ata
//
LOCUS       DAGCYL010000188         4334 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.191, whole genome shotgun sequence.
ACCESSION   DAGCYL010000188 DAGCYL010000000
VERSION     DAGCYL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.191"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            29..499
                     /locus_tag="MB216_002482"
     CDS             29..499
                     /locus_tag="MB216_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBT4527264.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            584..1192
                     /locus_tag="MB216_002483"
     CDS             584..1192
                     /locus_tag="MB216_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBT4527265.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            1326..1526
                     /locus_tag="MB216_002484"
     CDS             1326..1526
                     /locus_tag="MB216_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4527266.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            2280..3506
                     /locus_tag="MB216_002485"
     CDS             2280..3506
                     /locus_tag="MB216_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBT4527267.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(3614..4084)
                     /locus_tag="MB216_002486"
     CDS             complement(3614..4084)
                     /locus_tag="MB216_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBT4527268.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         1509 a          643 c          852 g         1330 t
ORIGIN      
        1 aagaattttt aataaggtag gttggctcat gaagcagtat cgacagttaa aaggatattt
       61 agaagcaaaa ggatattggg agatactagt ctatctcttt tttggtggtt tggctacgat
      121 tgtaaatttt gttagttttg ctctcgcacg ccaatatttt gatttaagta tggccttatc
      181 caattctatt tcgtggtttt gttctgtatt gtttgctttt gtaacgaaca aactttgggt
      241 attccattca aaatcaccga attttacaca tgcacttatt gaatgcggta agttcttctt
      301 ctaccgtatc ctttcttacg gattggacat gggggcaatg gtattgttga tcaatgttat
      361 gaacagcaat gagtatgtgg cgaaaatcat cactcaaatt attgttatcc tagcgaacta
      421 tattttcagc aagttgttta tttttaaaga aactgaggtt tttgaagaag aagtaggtca
      481 aagtgaaaaa gacgactagg cataacaatt gaagaatttc cttctctttg ttactctttg
      541 taatgtagac attataaaga aaacattgag gaggaatttt tttatgactt acacattacc
      601 agatctacct tatgcatatg acgcattgga accttatatt gacgaagaaa cgatgcatct
      661 gcatcatgat aagcatcaca atacttatgt gacgaattta aatgcagcaa ttgagaaata
      721 cccagaatta ggcgaaaaaa caatagaaga attattatct gatatggacg ctattccaac
      781 agatatcaag acagctgtac gtaacaatgg tggcggacat gctaaccatt catttttctg
      841 ggaaatcatg gcaccaaatg ctggtggcga acctacagga gaaataaaag aagcgattaa
      901 tgaagctttt ggtgattttt cttcttttaa agaagaattc aaaaaagcag ccgctggacg
      961 atttggttct ggatgggctt ggcttgtaat ggaaaatgga aaattagcta ttacctctac
     1021 tgcaaatcaa gattctccat tgatggaagg aaagacacca attctaggtt tggacgtttg
     1081 ggaacatgct tattatttga aatataaaaa tgtccgacca gattatattg cagcattctg
     1141 gaatgttatt aattgggatg aagtaaacaa acgtttcgaa gcagcaaaat aaccatttcc
     1201 tccgtcatat cagtcttaaa aataatttgg aaaataaaag agcgaaagct tgctagcgtt
     1261 ttctaaattg ggtataatgt tcttgcaagg ttacttgcaa gaactttttt ggaggctata
     1321 catttatgga acacggaacg gttaaatggt tcaacaatga aaaaggattt gggtttatta
     1381 cagtagacgg cggagatgac gtatttgttc atttctcagc aattcaagga gacggcttca
     1441 agagcctaga agaaggtcaa gaagtagaat tcacgattgt tgaaggtgca cgcggacctc
     1501 aagctgctga agtgactaaa ttataataat ttgttgaata aagacacgac ttcttagaga
     1561 gtcgtgtctt tttgtctatt tatgaagttt ttagattttt tggctaagta aatgcatttg
     1621 ttcacttgac atgtcctaac taattatcaa gcaaggaaca ttttcttcgt tagtaatgga
     1681 ttttaaagaa atttcagttt ttggatgttg aataaaagac catttgtgaa accactagta
     1741 tacatactca ttaccatgtc aattctatta ctttaaaaga aaatcgtttt actttgaaga
     1801 ggagcatatc tatccattaa aagatgatgg taatgaaatc actagttcga gtcattttaa
     1861 gagccatgca gtgagctgga aataaaacga atagaagatg ggtttaacaa aaggaccagt
     1921 cataggaaga aattttcgtg aaatcgaatg ataatctgaa cggtaggcaa agactgttaa
     1981 aaagatggga aaggtatgtg cgatcatttt actgcacgat tatttggaaa ataccttgtt
     2041 gttcgctttt tctggaaaaa atacgtaaaa aaagataaaa cgattgcgaa ctgcgtgtat
     2101 tcggtctttc gatctttttc acggtatggt atgatagtta agaattttga gaaattttaa
     2161 aaaatagtaa tcgttacaca aaaatgtttt ttaaagtttt cttatgatta aaaagtgaat
     2221 ttgctgattg aacagttata tgagacaata ttttagaaaa tgtgaaacag aggtgtatta
     2281 tgaagcgatg gcaaaaagtt ctattaacct tgttaggggt atttgttttg ctgattgccg
     2341 gattatcagc atacggaatc agatttatgg gagaagcgag ccaaacagta aacaaaattt
     2401 ctaaaaactc taatcgaata tcctccaaac gttctgagag agtaagcatt gatgacaaag
     2461 aacctttttc ggttttgcta ttaggtttag ataccggtgg attaggaaga acggaacaag
     2521 gacgttctga tacgatgatg gtagtaacag ttaatccgaa acaaaaaaaa tcaacgatca
     2581 tcagtttgga ccgagatatt tatacgaata tcgtaggtta tggaacggta gataaattaa
     2641 atcatgccta tgcatttggt ggtgtcgaaa tggccatgga ttcaattgag caactgctcg
     2701 atattcctat tgatcattat gtaacgatca atttggatgg catggaagat ttgatcaatg
     2761 cggtcggtgg catcaaagtg aataataaaa ttgactttac tttggatggt gtccatgtac
     2821 cagtaggtga acaaacattg aatggagaaa aaggtttagc ctattctcga atgcgacacg
     2881 aagatccaga aggcgatatc ggtcgacaac gtcgtcagcg ggaagtagtg acgaaaatcg
     2941 tcaataaagt tttaagttta gatggcgtaa gcaattatag aaaaatttta aaagcagttg
     3001 agaacaatgt gacgacggac ttagattggg atgacatgct tgatgtagcg acgaactaca
     3061 cccctgcgtt tgaaacaatc aaacaagacc agttgcaagg cgaaggacaa atgatcaatg
     3121 atatctacta tcaaatttta ggaaaaaatg atcttctttc gatccaaaat gaactgaaga
     3181 aacaattaaa tataaaaaca agcgatacat tacctaattt gaaaaatgag aatgcttcaa
     3241 tgatgttctt tgatgattcg gaagatggcg attcgacaaa cggtgatgga tcgaatagtg
     3301 aagggtacac cgattattct tcctattcag aaaacacata tgatcagtac caagatccga
     3361 atacctatta tcagcaaaca gaagatacaa gtcagtacta tacaccaagt caagatactc
     3421 aaagttatga tccttacgct ggacaggaac aaaatggtta tggttatgac cagaataatg
     3481 agtatggaac aacaagtggg tactagaaaa aaatagtttg gttaataaag agtgccacct
     3541 aaattagaca aaaaatctaa cttaggtggc actttttcta tgtgttaaat tacaggacgt
     3601 ctctttttag cgcttagttt aaaaaataaa ttgaataatt caatagagtt gcgaatagta
     3661 cccataaata ataaggaatc atgagccaca ttgccaggcg atgatgtagc cacaactgat
     3721 acataagcac tgacaagaaa ataactaaaa gtgtaatatc gattgctgca atgaaaatat
     3781 ttcttaaatt aaaaaataga gcagaccata taaaattaaa aatgaactga atcacaaaaa
     3841 gaccgacaag attgtttcgc tgttttttgt tgttgtgatt aagtaacaaa taaagagaaa
     3901 tacccatcaa tatatacagt aaggtccaca tcggcccaaa tatccagcta ggtggtgcaa
     3961 atggtggaag ttgcaaagaa tagtaatatt ctccgggatt tccgctaagc aaccctgata
     4021 aaaagcctaa tatgacaatt cctataacac aaacccaaaa acgaatatct tttatcatct
     4081 tcattttact aactcctttc tataatagta gtttaccatg aaggctcaca gcaagacctg
     4141 tatttagttt gtgaagtaag aaaaaaattg atgattgttg ttattaaaaa gttgaacatt
     4201 tgtttatatg aatgatgttt ttgaaaaaga tcatatgatt tggttctcat gttttctatt
     4261 tgtacttttc tcgtctactt ctgttataat ctgcatctta cttttttcac cttttttact
     4321 tcaacataaa atta
//
LOCUS       DAGCYL010000189         4282 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.192, whole genome shotgun sequence.
ACCESSION   DAGCYL010000189 DAGCYL010000000
VERSION     DAGCYL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.192"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..278)
                     /locus_tag="MB216_002487"
     CDS             complement(<1..278)
                     /locus_tag="MB216_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527269.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLK"
     gene            complement(486..1412)
                     /gene="glsA"
                     /locus_tag="MB216_002488"
     CDS             complement(486..1412)
                     /gene="glsA"
                     /locus_tag="MB216_002488"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBT4527270.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1435..2862)
                     /locus_tag="MB216_002489"
     CDS             complement(1435..2862)
                     /locus_tag="MB216_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4527271.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2893..4107)
                     /locus_tag="MB216_002490"
     CDS             complement(2893..4107)
                     /locus_tag="MB216_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBT4527272.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1341 a          914 c          766 g         1261 t
ORIGIN      
        1 aactttaaaa aataagattg accgtacaga aactgtgtga gctcagaaag agcaatacca
       61 tgtttgaata cgagtttata cttatgcttc tctcttactt gttcttttat acaatattct
      121 agacactcgg ctagactttc tagatcgtga aggatcgttg gtgtagtcac ccttagagaa
      181 atagctgttt gttcgatgtc tatttcttgg aattcaaata attgttcaca cagatacact
      241 tttctaaata tatttcgctc gatgtaattt tccagcattc ctgatacacg cctccagttt
      301 ttcttatgat tttatttaat catatctgta aatcgtattt ttgtttcctt tttatttaga
      361 aaagcccttt caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac aagctcacaa
      421 tttcagcctc attgtaggta gctttgagcc gtttggactt ttaccccagc ctctttcaag
      481 aatggttatt caaagatgtc agcgtctagt tctttgacta catctttcaa caattggata
      541 ccagctgcgc tgttaccaaa tggatctagt gccgggctga agacacctat gccgtaacga
      601 tttggcacag ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac accaacatgg
      661 acggaaaact ctcctgattc atcatacaaa ccagcagttg tcataattga tttgacgatt
      721 gtagcacttt cttcagtaat gatttgtttg ccattccaag gagcaattcc tttatttgcc
      781 agcacagcag ctaattttgc tatgcctgta gcggtgacat tgagggagca ttgtctgaaa
      841 taagcatcga gtatctccgg tacgtctcct tcgatcattt gattaccttt catatagtaa
      901 gcaagagagc gattgatatc acctgttctt gattcagaat gatagatttc ttcattcaat
      961 gtgatattag gatcatcaca gatctctttc ataaatgaca agattcgctc aaatttttca
     1021 tctgcatctt taccggcaat caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga
     1081 ttcattggat gtttgcgatg attgatttcc atattcatga tagaattgaa ggcaaaaccg
     1141 gttggttccg tgttgattgt ttgaaagact ttttctattc cgttgtcttt gattgctaat
     1201 aacaatacgg gaacttttga catgctttcg atggcaaagc gcacatcggc gtctcctccc
     1261 tcaatcagtt gttttgtatc caaatcataa atcgccatac ctaattgttt gggatccact
     1321 tcagctaatg ctggaatgta atccgccact tgtcctgttt gataatagtg gaaatttttt
     1381 ccaatcaatt gttctaattc ttttacttcc atttgaataa ccagctttca ttttttattt
     1441 ttctgcatgc tggaatttat gacggcatgc gtagatcgta aatggaataa ctaagacgat
     1501 aagataagaa acaatcagaa tcgtttcata aatcccatgc tgtccagctg cgatactagc
     1561 aggtggaaca aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac
     1621 gattactttt acaacttttc cgcctggaac ctgataggct ctttcaagac tgtcatcttt
     1681 aaaaaccagc ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt agattacaac
     1741 agtcaatgac atcgctgcaa gaaatgaaag attattcccc ccgccgctta atgtaaagac
     1801 ggctgcccag attgttgcaa cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg
     1861 tgtgttttct tttgcaaaat attcaggaag taatccttct tctgctgcta cgtgaagtgc
     1921 ttttgaagga gaggttaccc aagaagcaat ttctccgatg acacctaaac aaacaagaat
     1981 cgcaagaatt ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata ggaattctaa
     2041 cgattgaatc acccctgtat taagagacaa accatgttga ggtactgtag tagcgacggt
     2101 caatccgcca aaagtatcta aaatgattgc cagaataacc aatacaaaca ttgctaatgg
     2161 ataatccttt ttagggtttt ccatttcatt tgcatgactg gcagatgttt ctacgcccat
     2221 atatgctaaa ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat
     2281 caatgttttc atagacagta tcgtttgtag tggagcgcct gaagcgacat aatggatccc
     2341 taagccaaac aagactaatg ctggcaacaa gataccagct acaaaaccga tccgagcaat
     2401 caatgaagtg tatttggttc cttttaactg tgagagtaaa atagcccaga aaataatcaa
     2461 taggattgcc aatttcagat atttattgtt ttgtatagca ccaatacccg ttgcatagga
     2521 taatgcacca gttaagaaat acagcatggt caagtagcca actgttatct caaaccattg
     2581 gaaaaagatt gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc aagtatagac
     2641 accgcctttt tcccatcctt tcaccgttgc catctctgct gcgcacaatg ccacagggat
     2701 aaaccatagc aaaccgccta ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa
     2761 tgtgggatat tcatataatg aaacgaccat tgagatggtc atagccaaaa agccaaagaa
     2821 cgttaatttt tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc ctcttttcta
     2881 tttgaatttg tttcaattga gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg
     2941 ttcttctttt gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt
     3001 cagcacatag gcatacacgg cagtaatgat gacaccaatc acttgaatcc ctaagaagta
     3061 ccagctgctt ggattcgtta gtaaatggct gttagatgca aagatcccta tcagaatcgt
     3121 acctgtgaat ccgcccatgc catgaacacc ccacacatca agtgcatcat cccattgttt
     3181 ctttttacga aaatcaacag cagcatgaca gacgataccc gcaatgatcc caatcagcat
     3241 ggcacttttt gcttctacat aaccggcaca aggcgtaatg gctgccaatc cagcaacgga
     3301 tcctgttagt acgtcgacaa aatcaaagcc atttcctttt acttttgcaa tgatcagcca
     3361 agtaatcatc gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga ctgcttgttc
     3421 atctgctcga agtgcaccac cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt
     3481 accgatagca gctgccataa gactgctatg ctgacttctt tctcctaaag gaatctttct
     3541 ttgaccaagt gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc
     3601 tccaccagcg aaatctctaa atcccatctg atccaaaaat ccgccacccc atatccagtg
     3661 gcaaacaggg aaataaatca gcagattcca aaaaataaca agtataatat agccttttaa
     3721 attcaatctc cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga taaacatcag
     3781 ttggtaaaga aagaacatta aaaatggaat cgttgcacca tgaaacatgt tggggaagct
     3841 tgtaacatgt ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc cgatatcccg
     3901 tccaaaggcc aatccgaatc caccaaatac ccacataatc gttgttatcc caacggagag
     3961 aaaagattga gacatgatcg ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa
     4021 agctagacca ggagtcatca ggcaaaccat tgctgtacat aaaatcatga atgctgtatc
     4081 tcctgtattg ataggtaatg tattcatttt tttcgcctcc tttctacgat tacgagtata
     4141 aaagaaggtg tagttgtttt tttcttctgt ttttttgaat taatacttgc atttataaaa
     4201 gacagacaat taataaaagc ttgatgtgtt gtgctctttt tcgtttttca cacaaataaa
     4261 gacaaaaaat aaacggtctt ct
//
LOCUS       DAGCYL010000190         4268 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.193, whole genome shotgun sequence.
ACCESSION   DAGCYL010000190 DAGCYL010000000
VERSION     DAGCYL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.193"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            42..197
                     /locus_tag="MB216_002491"
     CDS             42..197
                     /locus_tag="MB216_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527273.1"
                     /translation="MVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLLNL
                     FNRIVGA"
     gene            210..713
                     /locus_tag="MB216_002492"
     CDS             210..713
                     /locus_tag="MB216_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBT4527274.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            840..1877
                     /locus_tag="MB216_002493"
     CDS             840..1877
                     /locus_tag="MB216_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527275.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1892..3589
                     /locus_tag="MB216_002494"
     CDS             1892..3589
                     /locus_tag="MB216_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HBT4527276.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3582..4043
                     /locus_tag="MB216_002495"
     CDS             3582..4043
                     /locus_tag="MB216_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HBT4527277.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            4056..>4268
                     /locus_tag="MB216_002496"
     CDS             4056..>4268
                     /locus_tag="MB216_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527278.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIF"
BASE COUNT         1479 a          540 c          878 g         1371 t
ORIGIN      
        1 aatggttttt aaccaatgct caatctttag tcttactagc gattgtggtc attggtttgt
       61 ttttaggatt taaacgggag ttttccaaac tgattggctt cttggtgatt gctttgattg
      121 cggtaggtct ggtctttaat gcttctggtg tgaaggatgt cctactcaat ttgtttaacc
      181 gaattgtcgg tgcctgaggg ggaatcacaa tggaacagat gcgtgtttat attgcgaacc
      241 ttggaaaata caatgaaggt gagttggtgg gggcatggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacagatt ggattaaatg acgaatatga agagtacgcg attcatgatt
      361 atgaactgcc ttttgagatt gatgagtata cgccaattga agagattaat cggttgtgta
      421 acttagcgga ggaactagaa ggaacgccaa ttggtgaagc tgcttctgag attcaacacg
      481 ctttcttcaa ttctttcgag gaaatggtag agcatgtgga tgatattgtc tattatccag
      541 attgcgatga tatgagtgat gtggcgcatt acttgattgt agaagctggt gattttggcg
      601 aagttccaga acaattgaag ccgtatattg attttgatgc ttgcggtcga gatttggaga
      661 ttggaggaaa ttatttggtt actagtcgtg gagtttttga gtatccagaa tgaatcaaac
      721 actatatata gtgtttgatt agtatatgta agcaaaaaaa cacaatatat agagtgcaaa
      781 ctgaagggta atgtgatata atatttgccg aagaaatatt tttgaaatga ggtaatatta
      841 tggcaaatat tcaaacaagt tttattgatt tccataattc aattagattg gacgtagaag
      901 acaatacgct acttaaggac tataaagacc aagtaattga tggattaaaa gattacttac
      961 ctgatgatgt gaagtttgaa acttttttac agggaagcta ttctgtttat accggaataa
     1021 aaagctgtga tgaaaaaatt gattttgaca tcgatattgc agttgctttc gaaattgatc
     1081 atactgttta tgaagatcca agggagccta aactgtgggt aaaagaggcg ctagttgaaa
     1141 tttttcctaa tgctcaagtt aatttaaaag taccttgtgt aactgctaca tttactggaa
     1201 aaaagacaaa aaaaaatgtg cacgttgatg ttgcggttta tgctaaagaa gatgaaaatt
     1261 atttcttagc aaaagcaaaa gaattcagtg ctcccgaaaa ccgatgctgg gaggaagcgg
     1321 atcccaaagt attaaaagag aaaattaata gtcatgtagc agattcagat gatcgaaaac
     1381 aatttcgaag atgtattcgc tatttaaaaa gatggaaaga taacaatttt aaccaagaat
     1441 ataaaccaac aggaattgga cttacaatta atgtgatgga tacgttccta gttaataagt
     1501 ccactgattt tttaactaga aaagttcagt ataatgatat ggaatgtatg aagcagattg
     1561 tttcttcgct gaaagattca tttgtgtatg agtacagtga aacagatggt tggcattatc
     1621 gtctacatgc atatcttcca gtgaagccta acagtgacac ctattcaaaa atgacagtga
     1681 atcagatgag tgattttaaa aacaaacttt ctaaattata tgatgatttg attttcgcta
     1741 tcgatactga agatgagtat gaagctacaa aacgattaaa taatcaattt ggggaggatt
     1801 ttttaattat ttcagaagaa gaagttacag aaaaaaactt aagaaatgca tttgttacag
     1861 actatccgag tgcttaggtt ggtgttaaag attggattat actgatattt taaaagaaaa
     1921 gctaacagat gttgtgcgtt tattcgatgg taattactat ggggattttt catttgctac
     1981 aattggaaag ttgcgtttaa aaattgaaat accaaaacat tttccattgg tacttcctag
     2041 gatttacatt cataattttg atcatttagg actttaccta ccacatgtag aaaaagatgg
     2101 gttggtatgt ttttcttcag atataaatta tttagtagat gaagaaaatt cagaattggt
     2161 tttattgcag tgtgttaata aaactataaa aacaattgag gaaggtttga aagggttaaa
     2221 taaaaatgat tttcgaaaag agtttgttgc tttttgggac aaacaagaga atcaaatttc
     2281 gttattctac ctttgtgaac cagcgttgtt tatcaagtca gttgatacaa taatgtacgg
     2341 taagtctttg gttgctttgg ataaagagaa cgaattacaa ttaaatgcag cgagtagatt
     2401 gtttaatgga gagaaagaag atactcatga attagatgca tactatgttc caattagaaa
     2461 caagaataat attatgcccc cgaagaataa taaagcatgg tcaaagaagg atattttaca
     2521 aatagtaaaa aaaaatgtaa caggtgcaaa taaaagattt tttttaaaac ttgaaaaaag
     2581 aaagaataga cataatgttt tacttattct agatatccca attgaagaaa agtcaaataa
     2641 tgtcataatt ggatttcttt ttacaaaaca atctggatct ataaaagatt ataagggtac
     2701 gcctaggact gtattagttc agcgtcatga tgaattatat ttggtgaata gaacgagcgg
     2761 agaaaactat ggattgggtg ctaatatagc tattgttggg gtagggtcag ttggcagtaa
     2821 aattgctgaa aatcttggtc cattaggtgt gagaaagatg acactcatag acaatgattg
     2881 ttttcaagct gaaaatttgt atcgaaattc acttggtgca gatagtttaa aaaatgcccg
     2941 atctcagaat aattttttta aagttgattt aatgcaggag gagctcgaaa aaaaattccc
     3001 atacctttgt gttgaagtgg agtcaatgaa cgttttagac ttaattagta tgcaacctgg
     3061 ttttttcaag gaatatgact atgtttttgt ttgcatagga gatacaatgg caagcctggc
     3121 tttaaataat tatttttcca aaagtagtca aaaagttttc tattcttggc tggagccata
     3181 tggcattggg ggacacactc tatttataaa ttattctcta aatcaaaaag gttgtttcaa
     3241 ttgtctttat acagattcaa actctaaaaa taggtgttca aatagagctt ctctggtaaa
     3301 agaaggacaa atttttgaaa ggactttagc gagttgccgc tcaaaattta taccatataa
     3361 tgctattgcc tcgacggaat gtgcaattaa tgcaataaaa cttttccata aagtcaatca
     3421 aaataatatg accaatagta ctcttttttc atggatagga gatgggagta cttttgagaa
     3481 tttaggatat caatatagcg acagaaatac ttttcttaat ggagtcaata agtttgaaga
     3541 aagattcttt tctaaatatt gcaaagtatg tggggagtta gatgattaaa ttttcgtgtg
     3601 accgtattgt cattgaagta agggatgaag ttcttaagat atttgaacaa tatcggcagg
     3661 gaaaggatga cttgcctgaa gctggaggag ttcttcatgg aaaaagactt aagaatggga
     3721 gcataataat taccgatata acaacacctc aaataggaga tatacaaaaa agattactct
     3781 ttaaaaaaaa ccgagaaatt catcaacagc tttctaatga gaaatggata gaatcaaata
     3841 agttttctat tgcattggga gaatggcata ctcatcctga agctgtacca actccatcaa
     3901 atgttgataa gaaaagctgg agactgaacg tgagcaagca acatgatgat agagtttatg
     3961 tttttattat cgttggattt tctgatttga gaatgtggtt actgtcaaaa aaatcagata
     4021 ttaggcaggc tataaaacta taaaggagtg attaggtgaa atcacaaaag aatatttggt
     4081 ggtgggggtt tttagtagga aaggtaatga taccagccct tctcttaatt tctctaccga
     4141 tgattacttt tttctttacc aataaaaaaa tgagtaaaga agccgtgatt tttttctttg
     4201 gagaccaaaa agctaccttt attgaaacgt tagtaacatc tcttcagctt aatttgaatt
     4261 acattttt
//
LOCUS       DAGCYL010000191         4255 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.194, whole genome shotgun sequence.
ACCESSION   DAGCYL010000191 DAGCYL010000000
VERSION     DAGCYL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.194"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..334)
                     /locus_tag="MB216_002497"
     CDS             complement(<1..334)
                     /locus_tag="MB216_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HBT4527279.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQ"
     gene            460..870
                     /gene="tagD"
                     /locus_tag="MB216_002498"
     CDS             460..870
                     /gene="tagD"
                     /locus_tag="MB216_002498"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HBT4527280.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            1046..1459
                     /locus_tag="MB216_002499"
     CDS             1046..1459
                     /locus_tag="MB216_002499"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4527281.1"
                     /translation="MISISHSIKELFTIKDKNIEINSFQETFYKDKKAIVIEGPLVRT
                     FRHCLFCKESAKQIEKNGKKISMIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYL
                     VAPSCFISKQVHYTILEELTERQALKTIAKRCDFQ"
     gene            1616..1873
                     /locus_tag="MB216_002500"
     CDS             1616..1873
                     /locus_tag="MB216_002500"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4527282.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            1960..3198
                     /gene="istA"
                     /locus_tag="MB216_002501"
     CDS             1960..3198
                     /gene="istA"
                     /locus_tag="MB216_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBT4527283.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDGI
                     RAYTRESLKQYDLVEVADEE"
     gene            3188..3928
                     /gene="istB"
                     /locus_tag="MB216_002502"
     CDS             3188..3928
                     /gene="istB"
                     /locus_tag="MB216_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBT4527284.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
BASE COUNT         1435 a          821 c          778 g         1221 t
ORIGIN      
        1 actgataaat cataaagaaa aagattaatt gccacatgat taacttaata ttttcgctat
       61 atccatattt taaattcatt aaactgctta ctagcatcgc gccaataaaa gcaattaata
      121 gccaatcata tttttttaga tttttttctc taaatgcaat aattgcatct ataatgatca
      181 agacaactcc cgctattcca gcaggccaaa aaagtaaagt atcatatcta gtaggtaata
      241 gtaaatacgg gcctattgct cgtaacatta aaataaaacc aaaataaata aaataccaac
      301 ctttcacata ttttaaaaat ctttcattca acatttttcc tccatccacc tatttctcat
      361 aaatatttct tatccttttt aggataaact ggtcctttca attctaacaa aaaaatgcta
      421 aactaacaat gtatattaat gaatttcgag aggtgaaaga tgaaaagagt aattacttac
      481 ggtacctttg atttgcttca ttacgggcat atcaatttac tgaaacgagc aaaacaatac
      541 ggagactatc ttattgttgc cctttctaca gatgaattca attggaatgc gaaacaaaaa
      601 aaatgttatt ttagttatga aaaaagaaaa cagttacttg aagctattcg ctacgttgat
      661 ttagttattc ctgaagaaaa ttgggaacaa aaagtaaaag atataaaaga ataccatgca
      721 gattatttcg tgatgggcga tgattgggct ggacattttg atgaacttga acaactaaca
      781 gatgcacaag tgatttattt accaagaaca cctgagatct ctacaactca aataaagaac
      841 gaacttaatt ctttaaatag tgataaataa tattttttaa acaataaaag aacaatatat
      901 ctggataaga cgtaaaaaaa tggctctata atttctagga ctgatagaat tttctgtcgg
      961 tcctagattt tttgtaaaaa aaaacagaca taaaaaaatt ccgatagaat agagtcgtct
     1021 aaaacatcaa tctgaaagga agtttttgat atccatttct cattctatca aagagctctt
     1081 cactattaaa gataaaaata ttgaaattaa ttcttttcaa gaaacttttt ataaggacaa
     1141 gaaagccatc gtaatcgaag gtccattagt tcgcactttt agacattgtc tattttgtaa
     1201 agaatctgca aagcagatcg agaaaaacgg caagaagatt tcaatgatta tcttaaatcg
     1261 cagtgggaat aaacaaacct atttgcgtct tagaaaacaa cattaccact gccaatcatg
     1321 cgggaaatat tttactgctc atacgtatct tgtcgctcct tcatgtttta tttcgaaaca
     1381 agttcattac acgatattag aagagttgac cgagcgtcaa gcattaaaga caattgcgaa
     1441 acgttgtgat tttcagtaac gacagttcaa cggacattat ctgctctatc ggcaacgctg
     1501 cgacagaagc tagactattt acctcattgt ttactattcg acgagtttcg ctctctttca
     1561 attttccatg ggaagttctc cttttcttgt atgaattgag agacagggaa gctctttgac
     1621 attttgtcca gccggagaaa aaaaagattt gattgcttat ttcatgcgtt ttacaagaaa
     1681 agctcatatg ggggttcgat acattgtcac agatatgaat gccccttatt tttcattagt
     1741 caaagaaccc ttcccaaatg cacaaatcat cattgattgc tttcacattg ggcaacactt
     1801 gaacaagttt ttcgatcgtg ttcgaaaacg tgtgatgaag cagttgaatc aaaaatgtca
     1861 acggcacctt taaaatgtag gaaaacggca atttaaaatt gttggtttac ctacattttt
     1921 gtatgctctt cttatcccaa atgaaatgga ggctcaggga tgagaaaaga catacgcgaa
     1981 ggagtgatga tctacgtgat caatgaaatc aaacccaatt atgctgcact agcaaaacaa
     2041 tacgactgcg actaccgaac agttaagcat gcttacgaag aagcccaagt taaagaatca
     2101 aaacctccag aacgtaagaa gcgtccaagt aagttagatc cctacagaga aatcatccaa
     2161 gacaaaatca aagaccaatg cagtgcttac agcattttca agtttattga gcacaaagga
     2221 tttggaggaa gctacagttt agtaaagacc tattgtcgcc agattaagca ggcgaaagta
     2281 cacaaggcaa ccgtccgagt cgaacatagc cctgggcttt ctgctcaggt tgactggaag
     2341 gaagatatgc gactaatcag taatcagggc gaaatttttg agttcaatat ctttttgtac
     2401 gtcttgccat attccaagaa aaaatatgtc acactaactt tcgaccgaaa acaagacaca
     2461 ctctttgagt gcttagatga tgcgttttat caaaccggtg gcgttcccaa agaaatctgg
     2521 ttcgataata tgaagacagt cgttgaccat tctcgtagtc agttttccaa accgcatttc
     2581 aacaaacgtt tttatgcttt cagtaaggat gccggcttca caccaattgc ctgtcggcca
     2641 tttcgtccgc aaacaaaggg ctgtgtcgaa gctttagcca gaactgttga ccgcctacgg
     2701 gtctataatc atgaatttta tgatgccgtt gagctgtgtc atttagtgaa taacctttgt
     2761 gttgagttaa ataacgaggt ttctcaatca actgatcaaa caccagatga acgttggcag
     2821 acgaacgaaa aagagcacct gcatccatac caaaaaaacc ttttgaatcc ctattttgaa
     2881 gactttattc gccgtaaagt caccaaggaa gaaatggtca ttttccgtaa atgcaggtac
     2941 tctgtggcgc ctcgctatat cggtaaagaa gtcgaaatcg aactctccga taaagaagac
     3001 caagtacaaa tctattataa cggagaactg attcggtcgc atccattaac gacaaaacag
     3061 ctgaactacc acgaggaaga ctatttccaa atcttgaaat ccgatgtcat gagtcacaaa
     3121 gaagacgatg gaatcagagc gtatactcgt gaaagtctca agcaatatga tctggtggag
     3181 gttgccgatg aagaatagcc attaccaaga actgatgacc aacttagaga ctcttggttt
     3241 gaatcatatg cgggaatatg ttcctaacta catcgaagtg gccaatcgag aggagctttc
     3301 tttaacggaa gctcttctcg agctgacacg cagagaagtt gatcatcaaa ataagcaaaa
     3361 aatgaatcgt gtggtggcac gcgcccgctt ttccaagaga accagcctgg aagagtttga
     3421 ttttgccttc cagccaagta tcaataagcg cgaagtgcta gacttaaggc acatgggctt
     3481 tgtggaaaaa catgagaact tggtttttat cggcagtcct ggtgtaggca agacacatct
     3541 gacgattgca ttgggaatcg aagcctgtcg tcaaggctat cggacactat tcatcacctg
     3601 tcatgagctg cttttgcgtc tgcgaacagc ctatgaaaaa ggtaccctag agcgtgttct
     3661 caaacgatac gctcgctatg acgtcttgat tattgatgag attggctatc ttccgattca
     3721 aaaacaggaa gctgatttat tctttcagct tcttgccatg cgctatgaac accgctctac
     3781 gatcattacg acgaacattg tcttatctcg ttggggggag ttgttccaaa gttctgagat
     3841 cgcagctgcc atccttgacc gtttagtaca tcacgtcaag gtgttcaaga tcacaggcaa
     3901 gtcttatcgt atgagagaca agatctaaag tgtcgattgt cgacaattac tattgccgaa
     3961 aacctacatt tttatcctgc ccaaaaccta caattttaag ctgcccttga catcaaaaag
     4021 acacttcaca agctaagtgc tatcgacaat tgaaaccttt gtataaatta cttctataat
     4081 cagatgacga gttggattat acaacattca agaaatggca gaattttcaa tggcgctatt
     4141 taacagaaag tgaagtagtt gattgtctac cctcaatctc ttatgaactt aaaatggctt
     4201 agcagtatta tcaaaagctg cttaacaatt atcacaagaa acagctagct acttc
//
LOCUS       DAGCYL010000192         4207 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.195, whole genome shotgun sequence.
ACCESSION   DAGCYL010000192 DAGCYL010000000
VERSION     DAGCYL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.195"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            137..1438
                     /locus_tag="MB216_002503"
     CDS             137..1438
                     /locus_tag="MB216_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4527285.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1465..1725
                     /locus_tag="MB216_002504"
     CDS             1465..1725
                     /locus_tag="MB216_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBT4527286.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2724..3959)
                     /locus_tag="MB216_002505"
     CDS             complement(2724..3959)
                     /locus_tag="MB216_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBT4527287.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1214 a          673 c          869 g         1451 t
ORIGIN      
        1 ggattcttaa aaataaattt gattatttaa gagaatatat tgaaataaaa tttcttaatt
       61 atataattag ttatgaacat tctttgcgtt gtcatgcaat gaatggttca tattttattg
      121 aaaaggagtg gttttagtgg aaaagctgat gaaatgggtc gaagagaaac ttgtcccacc
      181 aatggcaaaa attggtacac agcgtcattt attagcaatt cgtaatgggg ttgtttcaac
      241 attgtcattg attttgattg ggacattttt tatggtcttt atcaatttac cgtttcctgg
      301 atggaatgaa tttattgccc cttattcggc aacgattgtt ttacctttta ggatgacgat
      361 gggattgatg gctatttatg caacttttgt tatgggttcg gatctagcta aatcatatgg
      421 attagatagt gtgacaggtg gtatattatc tttaggaacg ttctttatgt tacaagttcc
      481 tgtaaacgtt ttaactcctg aaaaggcacc tcttggctgg gtcttaccta tgagttcttt
      541 gggagcatct gggatgtttg cggggatctt atctatgatt tttgcagtgg aagtatatcg
      601 tttctttaaa aatcgaaata ttactatcaa aatgccagaa caagtgccac ctgctgttgc
      661 ccgtagtttt gaggcattga ttccaggtgc agtcatttta acaattactt ggctgattcg
      721 aagcgtcgcg ggctttgatg taaacgctac cttattatcg ttattcgagc ctttaaccaa
      781 tattttaggg aataatttgc ttggtgtttt actaccaatg tttttgatcc atttgttgtg
      841 gtcatttggt atccatggca tgagtattgt aggggctgtt gttcgtccaa tgtggctgat
      901 catgttggat gagaatgcaa aggcattggc agatggtaca gctgccaccc aattacccta
      961 tatcgctcct gaacagtttt atcaatggac ggtaacaatt ggtggtgcag gagcaacgat
     1021 cgtggtatct gttttgttta tattcttctg taaatcaaaa ttcttgaaag aggttggtcg
     1081 tttttctatt attcctggta tcttcaatat caatgagccc atgatttttg gagctcctat
     1141 gatgttgaat ccttacatgt tcatcccgtt caacttagtt cctttagttt tgactattat
     1201 cagttatttt gcagtcaaat tagaaatggt caatagattt actgttttgc caacttggac
     1261 attaccagct ccaatcggcg gttatctttc agcaggaaat gactggcgtg ttgttatctt
     1321 gatcatgatc aacacattga tagcatttat tatctattat ccatttgtaa aagcttatga
     1381 taaaaagatg ctgtcagatg aacaaagtcg tggagaaaac gaagtccaaa ctgtctaagt
     1441 ttggttttta gaaggtggga aacaatgtat tggctgattt tattttttgt ttttatcttt
     1501 ttattaacgg ctagtcacct tatattgaat atgctagcta catatcatat tcaaatcaat
     1561 cgatggattt gggcactggc atcatttttg atcgtgattc ttccaaagat cattgtaccg
     1621 catatgaatg ttttgttttc atgggggaca tatgtacttt gtggaatttt tgctatcaat
     1681 tttatgatcg agcagcatag atggtttgtc acatcgaagc tttaaatgtt ctaaaagtgg
     1741 ttgtgacaga agtggttagc ttcaagaaat aagaagaaat ttacgaaaat tgcttcttaa
     1801 atttttgtga aatgttggct tatttttcgt ttggttgaac ttattaccgc agatgcaagc
     1861 ttttgaccac cgtttattcg gaatatggga ctgtgacaag gcttttgtca cagccctttt
     1921 ttacataaag attattattg ttttaactgt tttggcagaa agaaggaggc aaaaatgaaa
     1981 attttatcag caatggatta agaattagtc gatctactac caaagtgatc aaggcaaata
     2041 tagatttggg agcttacaac catatagtag gggctgcagt atggtgtatg aatggtaata
     2101 tgtatgatga gagtgtttat tcaaatcatg aaacgtgtat agaaataatt gcgctgggaa
     2161 tagcaatcag tcatggtgaa cgtgactttt agtttatcgt tgctgacgac atagaacatt
     2221 ctgacatgat ctactcagca tgtggggatt gctggcagtt tatataggag tatgcatcgg
     2281 attgcgagat cattgtctca acagaagagg gaggttgatt atcaaagata aagtggtaaa
     2341 tcctacattt gatttagtaa ttgttgaagg gactagcagc acaaatctct acaaagcgat
     2401 gcttctagat ctatatgaca cattttaaga aaatgtattt gcctaattat tatggtcttt
     2461 cttttgaaga aacgttgaga tgatccaacc agagaaaggt gtcaaagaat ttgactaaga
     2521 ataaatggca gacgctacct atgctcattt ggattgcgtc agatctttta ttttaaggat
     2581 gattttgtgt tttttagaca ccgtaattta agtaactcta acttttcaat ccactattta
     2641 ctttttttgt attttcactg tctgggtctc gaatagcgaa attactgatg agtctctatt
     2701 aaaaaagctt tttctacttg aaactatatt gggtggttgc tcaggaaggt ttcgatttct
     2761 tgaattgctt gttgtggacc atttattgga aaaacggctt gcagcgtgcg tgcctgctga
     2821 cgaatcttgg gtgaagaaac ttcgcgcaga gcaatctgca gttttctttc agtcactggt
     2881 tttgtgaggg cgattgcgtt tccgtatttt acacaatagt caatattaca gctttgctca
     2941 aattgcatcc ccacaccgat aaacggtttg ccggattcac aggcagtttg gacggtccct
     3001 tccccgccgt ggataaaaga aaaatcaatg aattcatgca aaagatgact agacagatat
     3061 tctgttatta aaagatcttc tgagtcagat tgaatgtcat caagcagata gtcaataggt
     3121 gcaataaccg ctagatccgg ttgtttttgt aagaagaaca aaattttgcg taacatacgc
     3181 ggattgcctg aactacccat tgcgaaatag ataatacgtt ttctttcttg ttgctttcgc
     3241 tgtataaaag ttagcacttc ttcaggcaag gaagtactga gcttagcaaa aattggacca
     3301 acgattcgat aattttccgg cagctgttct tcttccaaaa atagtttagg cgttgtgatg
     3361 agatttagat cagcatccat catttgtaaa cttgtttttg gtaaaggaag catttctttt
     3421 tgtgcagcgt gtttgaaggc attggggatc catcgggtat ttactatacg cttttttgtc
     3481 cagtgccaaa ggggattgct taaagggccg aagaaacgta actcgtgggg catcagcgca
     3541 tgctctgatt gaaaaaaagg ttcactcaaa gcatagggtt taacatatac taaaggaatc
     3601 tttttattgc gggccgataa aaaaattgtc acattcgaac ctgtcacaat cagtcgaggc
     3661 tcaaacttag caatcagact ctgttcacta gtgatacgag aggcaaccat ttctttggta
     3721 aaagggtttt taagtgtacg caactggtca aattttatga tttgttcttc ttgttttttc
     3781 gtaaggatcg gcgctagtaa atgatactca aagccacttt ctgtgatcaa aggagcaaaa
     3841 gttttagaaa agccaaagaa tacacagtaa taacggtctt tcatagcttt ggcaacctca
     3901 atcattcgtg ttgtctcagc taaattgaat actgcaggag caaaaagaat tgtcttcatg
     3961 aaatcctcct tattggaatg agtctatccc ttcgtttctt actagtatac taaaaaacac
     4021 agcgttcgag tagcctatgt atattttgaa tattaaactg tctctcgctg ttttcacaac
     4081 ttctaaattt aaattttgta atcaggttga aactccaatt ttcaaaaaaa cacgcaacaa
     4141 ttttttgatc gatttatatg ttgtgccaga taaatacgag aagttgtgtc attaaaaata
     4201 aacatga
//
LOCUS       DAGCYL010000193         4177 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.196, whole genome shotgun sequence.
ACCESSION   DAGCYL010000193 DAGCYL010000000
VERSION     DAGCYL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.196"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..220)
                     /locus_tag="MB216_002506"
     CDS             complement(<1..220)
                     /locus_tag="MB216_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBT4527288.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEF"
     gene            complement(351..935)
                     /locus_tag="MB216_002507"
     CDS             complement(351..935)
                     /locus_tag="MB216_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4527289.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(1266..1499)
                     /locus_tag="MB216_002508"
     CDS             complement(1266..1499)
                     /locus_tag="MB216_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBT4527290.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1514..2461)
                     /locus_tag="MB216_002509"
     CDS             complement(1514..2461)
                     /locus_tag="MB216_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBT4527291.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(2461..3303)
                     /locus_tag="MB216_002510"
     CDS             complement(2461..3303)
                     /locus_tag="MB216_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBT4527292.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(3436..4161)
                     /locus_tag="MB216_002511"
     CDS             complement(3436..4161)
                     /locus_tag="MB216_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBT4527293.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIVHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
BASE COUNT         1395 a          772 c          511 g         1499 t
ORIGIN      
        1 aaaactctat cttgatagca tcaataattt tcattactaa aaacagaact gaataaatca
       61 agatgaaata aatacatgtc tttattttac gaaacatcaa tactaaaact gtagcaataa
      121 tgaaacaccc cacttgaaat atatttccct ttcctaaaaa aataatgacc ggaaaaaaaa
      181 tatttaaagt aatcaaggtt aacggattga tttttgacac tctttcacct ccaaaaaata
      241 agatcgcact gttatcttaa gctgataaaa atgcaccgtt attcagataa cagtgcgatc
      301 ttatcaaaaa gcataactga gaacaattct caatgcaagc ttttttttaa ttaactcagt
      361 aattcaatcc acccaggaac agaaaattga tacaatcttc tggttaatag catagtcttt
      421 tcttttgaat agctatgctt caatatatca tagaatgtag cgtagaatga gtaaatgtag
      481 aagtggattt cttcttcatt aaagactaaa ttttgattga tcattccgat agtctttaac
      541 ttatctagac aacagactgt cttttttgtc acaaatgatg taattttttc aacaatatct
      601 tgatatttgg ttccagctga ttggaataaa accaaatcga atatacgttt attctcaaaa
      661 atataattaa taaatggatc taattcattg aagttctctc tccaactttt ctctatatct
      721 atattttcta agtttaagag gtatttggta aactcttcat tatacatatt aataagcgca
      781 ttaatatgcg gctgaataat ttcttcaaat agcgcttcct tagaagtaaa atgtttataa
      841 aaagcacccg tagttacgcc tgcggtcaag cacagttgcc ttatcttagt ttgctgaaat
      901 cctacttttt caaaatggct tagtccactt tctattaatt tttggtgagt tgtaatataa
      961 tctctggttt ctatgggtac ttcctcctaa taagatgaat taactatatt atcttacaca
     1021 ggacttgaag tttcaatcgt taacctctat tactgaactc attccttaga gctaaactaa
     1081 tgagaaagag caaaataact gatacaaaaa gcaatcctac aagaattaat agactcatct
     1141 aaatcattca ccttttctaa aataatccga gaaagattta aaaaaattat atccagctat
     1201 taggataagt cctatcataa tcactccaat agccattttc ttatcctccc taaaaataaa
     1261 gccttttact taaaaccttt tttgtaatcg tcaataaatt ttttcccttt atcagttaat
     1321 tgaaatccta tatctaaata gtcctcatat ctaagtttag tcagcatctc acttacagat
     1381 gctggactaa cacttagaac ttttgctaaa tcattatttt ttaatttctt ttttcttttt
     1441 gataattcct gaattgctag taaataaatt agtatagtag ttgttttaat ttgtaccata
     1501 cctaccctcc aaattatttc gaaaggcctt cagcaatttt atccaggttc catttcatca
     1561 tcgcatagta actatcacca tcttgtcctt tttctgcaat tgaatcagta aaaatcgttg
     1621 aatagatagg aatattggta tcttttgata ctgttttcat tggtctatcg tccacactgc
     1681 tttctacgaa taaggaggga acttttgttg tgcgtaattt ttcaactaag tgttttattt
     1741 gatctggtgt tccttcttct tcagtgttga tttcccaaat gtaagcagaa ggcacattat
     1801 acgctttcga gaaatattta aagcatcctt cacttgtaac aatcattttt ttatcattcg
     1861 gaattgaagc aaatttagat ttagcttctt tgtctagaga atccaatttt tcaatatact
     1921 tatctagatt ttctttatag aattttttat tatcaggatc tttttccgct aactgttttt
     1981 caatattttt agcgtaaata ataccgtttt ctaaatttaa ccaagcatgg ggatcttcct
     2041 tccctttctc actctgaccc tctaagtaaa taacatctat gccatcactt gctgcaaaat
     2101 agtctttgtt ttcctctttg ttcgcatttt ttactaattt tgtaaaccaa gcatttcctc
     2161 cagtttccaa gttaacaccg ttataaaaaa tcaaatctgc ttttgaagtt ttttgaacat
     2221 cttcaggcaa aggttcatat tcgtggggat ctttcccaat aggtacgata ctgtgtaaat
     2281 caattttatc ttttgctata ttttcagtaa tatctgctaa aatcgaatta gtaactacta
     2341 cctttaattt ttcgttcgaa gccactgtat ctttgtcttt actatttgtt gaacatgccc
     2401 cgaatataaa aagagcaatc aaaatcccca gacttcccag tactgctttc cattttctca
     2461 tcataacttt ctctccttct ttcttaaaaa tttttgtttt ggtgagatta gaaaactaat
     2521 tacaaataag aatgccgctg ttaaaacaat acttgatcct gctgctatgt taaaactata
     2581 accaatgaac aaacctagca atgatgagca cgctccgact gtcgccgaaa taaatatcat
     2641 agtttttaaa ctgtttgcat ataaatatgc tgtagcagct ggtgtaatta acattgcaac
     2701 aattaatata gttcccacac tttgcatagc tgttactgct actaaagtta atagaatcat
     2761 taatagataa tgataaaaac tgacattcat ccccattgct tttgccatta atggatcaaa
     2821 agaagtgatt aataattctt taaaaaatag aacaactgta accaacacta aaacagaaac
     2881 gccaattgta atccacttat caatatcttg tacagctaaa atatttccaa ataagatatg
     2941 aaataaatcc gttgagctat ttgcaactcc tataaggata actcctaatg ctaaaaaaga
     3001 actgaaagta ataccaattg ctgtatctcc ttttattaca ctattgcttt ttataaaagt
     3061 aataatgata gaagctaata gaccaaatac aatcgcacca ataaaaaaat taatacctaa
     3121 aatataagaa attgctactc ctggcaaaac agcatgagaa atagcatcac ccataagaga
     3181 cattcctctt aaaataataa aacaacctac tgttccagaa acaatcccta taactacaga
     3241 tgtaattaga gcattctgta aaaagtgaaa gtcatacaga ccattaataa aattttgaag
     3301 catattattc cccttgtata ttagagttag ggttttaacc ttccggtttt gattggataa
     3361 ccctatgaac catcgttatt ttaatttggt attaactcat ttcttttgag ataatatatt
     3421 tattggattg agaggctaag ccgttccccc ctctccaata aatatagtat ctccataggc
     3481 tttttttaaa ttctcttcat taaatacaga ttctacactt ccgtgaacaa ttagatttcg
     3541 attcaaaaga ataatattat caaaatactt ctttaccttg cttaaatcat gatgcacaat
     3601 tagcactgtt tttccctgat gtttcaattc ttttaacgta tccataatta ttcgttcact
     3661 aactaagtct atccctacaa atggttcatc taaaaatatg aaatccgcat tttgagcaag
     3721 acatcttgct aaaagtacac gttgaaattg tcctccagat aattctccta tttgatgatt
     3781 agaatatttt cccatatcga ccttttccaa agcctttgaa actctttgcc attctgcatt
     3841 tttaattctt tgaaaaactt tcattccggc atatgttccc atagatacac attcttttac
     3901 ttttattgga aaagtaaaat caatatcagc tttttgctct acatatgcaa tcttcaccag
     3961 ttttttattg atatcttcat tattaatgga gacctctccc tgatgaggga taatatttaa
     4021 cattgcttta agcaatgtag attttccagc accatttgga ccaataatac ctgttaaagc
     4081 accttcttgt atttttaaag aaatctcgtt caatgctaaa aaatcgttat aacaaacatt
     4141 aagctttttc acttctaaca taactttctc cttctaa
//
LOCUS       DAGCYL010000194         4087 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.197, whole genome shotgun sequence.
ACCESSION   DAGCYL010000194 DAGCYL010000000
VERSION     DAGCYL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.197"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..508)
                     /locus_tag="MB216_002512"
     CDS             complement(<1..508)
                     /locus_tag="MB216_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBT4527294.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIR"
     gene            complement(607..957)
                     /locus_tag="MB216_002513"
     CDS             complement(607..957)
                     /locus_tag="MB216_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBT4527295.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1114..2643)
                     /gene="ahpF"
                     /locus_tag="MB216_002514"
     CDS             complement(1114..2643)
                     /gene="ahpF"
                     /locus_tag="MB216_002514"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBT4527296.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2653..3216)
                     /gene="ahpC"
                     /locus_tag="MB216_002515"
     CDS             complement(2653..3216)
                     /gene="ahpC"
                     /locus_tag="MB216_002515"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBT4527297.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3360..4022)
                     /locus_tag="MB216_002516"
     CDS             complement(3360..4022)
                     /locus_tag="MB216_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4527298.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
BASE COUNT         1265 a          827 c          698 g         1297 t
ORIGIN      
        1 ttctgatgaa attgataatc tcctggatgc ccatgaaaat caaaataaag ccgaatattt
       61 ggaatagaag catcaaactt ttgaatggat tgaacaataa gacgaatccg ccaatcaaca
      121 acagaatact actataaaca ctccaacttt tagcttttgt attcatgctg aagaacaact
      181 ggaacaagct gttgatgata acagctaatc caagaagaat cggcaggata gaaacgattg
      241 caggtgcaaa agcatagacg atcagagcta agatcaggaa aaaaagacca ctgaacagac
      301 cgatactcca agaacctgtt ttccttttaa aacggtaagc ctcaaaaaga ttcaacaacc
      361 caagcaaaac aagataggcg gtgatcagat aaccgataaa atgaaaaaca gcagaagggt
      421 ttagcacaat cgcaatccca gaaataatat aaacagcagc tcttaataag gcgtattttt
      481 gaatattttg tttaatcgtc tcaaacatat aagaaaacct cctttgactt attttaacgt
      541 gaactagtca ttttaccaaa ttaaatgtta caaaatgagc gaaggagaag aatatctctg
      601 gaaaaatcaa cttctgagga tcttttccat ttttttgcct tttgctagct catcgatcat
      661 tttatctaaa taacgaattt ctttcatcaa gttatcctcg atgtcttcta ctcgtacacc
      721 acaaacaaca cctgtaatca aggaacgtga aggattgggg acaggggctt ccgcaaaaaa
      781 ttgttcgaaa gtcacttgtt tttgtagttg atattctagt tctgtttggg aataacccgt
      841 cagccaacaa atgatttcat ctacttcctc ttttatacga tcttttctct ccgcttttgc
      901 tacatacata ggataaacgc ttgcaaaact tgtcgtaaaa atacgatttt gcgccataat
      961 cagtcacctt tctttttttc aaattattta ctacttatag tatataagaa aaagagaaga
     1021 aaaagcgatt attcaaaaaa acaaaaagga ttgtgacaaa attcttgtca ccatcctttt
     1081 ttccgctctc tgacttttta caatcttcat agctcaagtg cgcatcagat aatcgaatgc
     1141 tcctaaagct gcagtagctc ctgaacccat tgaaacaatg atttgtttgt atggactatc
     1201 tgtacaatca ccagctgcga aaacgccggg gatattcgtt gcgccatttt tctcggtcac
     1261 tatttcattt cttggattca tttcgatcgt tctatctagc caatccgtat tagggagtaa
     1321 gccgactaga atgaatactc ctgaaacagc tagtgtatgc atctccatcg tacggcgatc
     1381 gatataggag agagattcta cctgattcgt accgctgatt tctttcgtcg ctgcatgttt
     1441 gataacagtg acattcgcaa gagagactag tttatcctgt aagatttgat ctgcttttaa
     1501 ttctggtaag aattctagaa catatacatg ttttgctaac ccagccaaat cgattgctgc
     1561 ttcaacacca gaattaccac caccgataac agcaatctct tttcctttaa ataaagggcc
     1621 gtcacagtgt gggcaataag caattccttt atttttaaac tctttttcgc ctggtacatt
     1681 gatagaacgc cagtgcgcac ctgtcgcgat gatggcggtt tttgctttaa gtgatatacc
     1741 gttagctaag ccaatattaa taaaatgttg ctctttattt aaggaaacgg cctgttgatt
     1801 catcataatg tcgatagggt aactacgcac atgactttct acttgctgca tcaattttgg
     1861 accttctgtg taagaggtac caatcatatt ttcaatccct agtgtttcaa cgacttgtcc
     1921 gccgaaagaa tcagcgacaa gtcctgtacg gattccttta cgtgctgcat agatagctgc
     1981 actactagct gctggtccac cgccaatcac taatacatca taaggttctt tttcaaacaa
     2041 ctcttcttca atcaaagggc cagtgatttt ttcaacaatt tgttcgatcg tcatgcgtcc
     2101 gctactaaat tcttctccat ttaaaaacac agttggcact gccatgattt ttttatcatc
     2161 gatttctttt tggaacatcc caccttcaat catcgtatga gtaatatttg gatttaatac
     2221 agctaatata ttcaatgcct gtacaacgtc ggggcaatta tgacaagtca agctaacata
     2281 actgtcaaaa tgcaatgttt gtgttatttt tttgatttta agactcaaag attcactgat
     2341 tttcggtgcc cgtccactaa tttgtaacat agccagtaaa aaagaggaaa attcatgacc
     2401 gagggggata ccagcaaaaa cgattccact ttctttgcca actggattga tagaaaaact
     2461 aggtgtgcgc atcgaatctc ttttttccca agagatttta ggagacattt ctgctatttc
     2521 cttcgtaaat tcagctagtt tttgactatt ttcactatta tccaaactaa cagaaaaaac
     2581 aataggagat tcaatcagtg ctaaatattg ttcgagattt tgtttagttt ctttgtctaa
     2641 catatccacc acctaaatct ttccaacaag atcaaaactt ggtttcaatg tttcgccact
     2701 ttctttccat tttgccggac aaacttcacc tgggtgggta cgtacatatt gagcagcgcg
     2761 aatcttatca atcagtgcac tagcgtcacg accgattcca tctgcattga tttccatagc
     2821 ttgaacgatt ccgtctggat caataataaa tgttcctcgc tgagctaatc cttgctcttc
     2881 atccaaaaca tcaaaaatac gagaaatctg atgagacgga tcaccgatca tcacgtattc
     2941 gattttaccg atagcatctg atgtatcatg ccatgctttg tgagtaaagt gggtatcggt
     3001 agagcaagaa aagacttcaa cacctaaaga tttcaatgta ctgtattgtt cttgtaagtc
     3061 ttctaattca gtaggacaaa caaaagtaaa atcagctgga tagaagcaaa gaatactcca
     3121 gttccccata agatcttttt cactgacttc taaaaattct ccttgatgat aagcatgtgc
     3181 attaaattct tcgatttttt tcccaatcaa tgacatagta aaacctccaa taataattat
     3241 ttatttataa ttattgtaat ttagttggag ttgcaaagtc aatggatatt ttgttattta
     3301 ataaagatta ctcaaaaacg aaggttaatt ataaaaatag aagaggtcgc aagaaaatgc
     3361 tactgctgga aatactcggg ataacgttga gtaacagttt cctgttcttt cagcatcatg
     3421 tgtaagtgca atcccgtttg aaatcgagct tcttctggtt ttttatcaac taaagccttg
     3481 agtaaatcat aatgttgttc catgatcgtt tcccatggaa gcccctgaat ggtcactcgt
     3541 aaccagcgga aacgttctaa atgggtatta tggtcatcta accagttcca aacttctttt
     3601 cttcctgcaa tttcgaaaca agcttcatga aataaattat ctgcacggaa aaaagcgcgt
     3661 tcgtcattta ttttgactgc tttttcttgt tcttctaaaa tcgtttctag cactattcgt
     3721 gtttgtgtgt ccaatttgga acaacattca atcatgattt gctcttccaa ctgttctcgg
     3781 acaaagcgag cattttctgc agaaacaaga ttaattttgg aaacatacgt accactttga
     3841 ggtatcgtat acacgagttc ttgctgacgt agttgaatca atgcttcgcg aatgggagtc
     3901 cggccgatag caagatgttc ctccagtgcc ttttcagata ttttccttcc aggttctaag
     3961 tcagcataga taatttgttt acgaatcgct tgataagctt gtgattgcaa gctgcttttc
     4021 atctttattc ctccaaaatc atttctatga acgtagtata acagaagagg tctgaaaaac
     4081 aatctgg
//
LOCUS       DAGCYL010000195         4030 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.198, whole genome shotgun sequence.
ACCESSION   DAGCYL010000195 DAGCYL010000000
VERSION     DAGCYL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.198"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(241..390)
                     /locus_tag="MB216_002517"
     CDS             complement(241..390)
                     /locus_tag="MB216_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527299.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            852..1064
                     /locus_tag="MB216_002518"
     CDS             852..1064
                     /locus_tag="MB216_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527300.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1342..3141)
                     /locus_tag="MB216_002519"
     CDS             complement(1342..3141)
                     /locus_tag="MB216_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBT4527301.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3265..3861)
                     /locus_tag="MB216_002520"
     CDS             complement(3265..3861)
                     /locus_tag="MB216_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBT4527302.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          712 c          688 g         1181 t
ORIGIN      
        1 tattaaatat tatacagata ataacggaat attatcatcg aaacagctaa caaaattgtc
       61 ataaaaaacg agatggttga tattagaact tgtgtttttg tttaggtcta gagataaaga
      121 gagacaattt ccaaatatat gtaatgaata caaaaagacg tttgtcgcgg attaccgtta
      181 caaacgtctt ttattgattt tccgagatct catcagtcgc catttggaat ggttcactcc
      241 ttatctagat tcgtttaatg ctcggactgc ttgttcgatt gtaataccaa acgcaacgtt
      301 attttcgttt tgtgcatcaa tcgccataaa acggttagaa gttgtatcaa aagctacgcg
      361 gtattgcata tttgtttttg taaacgtcat aaaattcatc tcctttgtaa tgtaatttag
      421 ttcttgaaag tttcctttaa actttcgaac ttataccaag tttagcatcg tacgtttttt
      481 gatgcaatta aaaagcattt atcattttcc ttatttattt tttcttaaat ggtttagcta
      541 ttttctttta atccgataag tagcccaatc atcaaggtta caaatactat tgcttgaata
      601 taaatcatat gctcacctct ttataaataa cttagcaata taaagaaaaa aacgcacgaa
      661 caacatagga atataaacaa acgtttttgt cacaacgtct aagatcgtgt taaaaaatag
      721 ttatctaaaa agcagaaaga tactcaaaat aaaaaataaa aaaatggata aaaacgtttt
      781 caaatacaag gtaatttgtt attataaaat aaagaaaaaa caaaataaaa ataaagaaaa
      841 gagggaatga aatggtaaaa gcagaagtag gaagatctta tatatgcaaa ccgatcggag
      901 tatctcatga actaatcggc tgtattgaac gtacatatat caacacggct gtcgtgtatg
      961 ttgaaagtta tgaccaacga gactatcaac ttatcaatga aagcaagtat cgcatgctcg
     1021 taaaattctg tgatattagc gcacctgctg aattggtttc ttaaacagta tttttgagaa
     1081 ttcgcaagaa ctcccttata atataaataa agacagaagg gagcgagaag atatgccaaa
     1141 aagaaaaagt tcagggaaaa tgggaattat tcttgcagtg atctctgcaa ttcctgctat
     1201 tttagctatt gttcgtgcga tgaaggaatc aagagaaagt taagcacaca ttatgagcgt
     1261 caaacaaaaa acgcatatac ctgcggtggc aggttatgcg ttttttgttg tataaagact
     1321 tttttcatta acgatttttg attagccgac tcccaaaaca aaatttagca gtttatctga
     1381 ataagtgatt tcttcatctg tttgtttcac actttcattt agtagtccca tctcatcggt
     1441 tatgatacca tccacaccat aaaacatcat gcgactcata gcatctgaat cgttgattgt
     1501 ccatacatat acttcttttc cgtcttggtg ggcagaatta atgaagttac ggttgatcgt
     1561 actatattcc attgtcagaa aatcagcagg cgtcacaggg ggcccaacaa tattgaaagg
     1621 taagatgtag cccacataaa agtttggttc tgcttctttt aaatctgaaa cggtttggta
     1681 cgtcaaactt tgcaaaatat gatggttctc taagataact ttgcggtatt tatggacaaa
     1741 tcgttcgaca aggtcatccg aatctttttt agaggtcttg atctcaataa gtaatttttg
     1801 gtttagctta tgtgcttttt ccaaataaga atcaaaagaa caaacagggg cttgcactcc
     1861 gttttcagta actgtaagat ttgtcaattc tttcaatgta agatcctgtg gtgttccatt
     1921 tacccctgtt aagtgacgaa gatttgcatc atgcatcact acgaaatgac catcttttgt
     1981 ttcctgaaca tccatctcta cataatcagg atgatagtct ctgcttgttt tttcaagtgc
     2041 ttctaaagta ttttgcgccc cattttgggt actcacacct ctgtgagaga cagtcactgg
     2101 ttttgtctga acagctgaag tcagataatc catattgtaa agacagacgc caataccgaa
     2161 aaaaactgca aaaagagtca aaccagtgtt tttcagcgca gagaaacgca agttgggggc
     2221 ttgtgggata aaccacttag ggatctcagg caagaagcct tcgtcatcca tgaaatcaac
     2281 aatgatataa aaaatcccta cggtagacat cacgatattg aataataaaa tcccttgtaa
     2341 aagcgtcata gcaaacacag cgctgatcaa tgcatagtct gggaaaaatt gttcaaccat
     2401 actttgtgcc aaaataacaa cgatatatcc taaagaagaa aggagtaaaa tcgtaccgcc
     2461 aatcacaata aattggcccg tgatggccaa aagacgcgat ttggtcaatg accagctttc
     2521 ccgaatggct gctcgaaaag gacgatcccg tagaatcatt tccggtaaag caaaaatcag
     2581 tcgaatccca atatagccaa ggaaaatata aacgagtaaa aaggaactga cgatgatcca
     2641 acgattagca aaaataaaat ccataatgaa agcggggatt tttatttttg acagtagatc
     2701 agaattaaaa cttaatccgc tgatcggtag aatcaataaa aaataagcaa ggaaaaacaa
     2761 aaaagtaatg ggtctcactt tcttcagctg aagtatcgtc aagtgtaaga gttgcttcaa
     2821 agagattggt tctttctttt taataaaaaa aacactcatc aaaagaaaag taaattcgaa
     2881 ataaactaac agtaaaatcg tcatcaaaac tacgattaac gagagtagaa ccccaggatg
     2941 ttgagaaaaa attgaaggaa tattgtcata agacaaatag tcaattgccc ctcggtgtaa
     3001 aataaaacgg gtcgtactgc tcaataaggg taaacaaata aataaaataa agccatgcat
     3061 cagcaagaca tcacggaaat aagcttgtgt tccttttaaa aagtcccaag cattacctaa
     3121 gctacgtttc aaataattca tcctatcacc tgctcttcac attatagcgc aaatctaatg
     3181 aaaaaggaaa gggtgcagtg tgttttgcag accctttccc agatttaaca ttgtcaatca
     3241 atgactaatt ttacaaaata aatattatcc aagaagacca cttagatatt gacctccata
     3301 aatcaaggcc atactatata agaaaatcga cagtggttta gcaatcaaaa tgattagtgt
     3361 atatcgtttc actgacatgt ttgtcaatcc agccatcaaa cataacgcat cgtcaggagc
     3421 aactggaaga aaaatagcta gagcaaacag cttctcaaaa cggttttgat tatccagcca
     3481 gccaatatat ttgttatatg tcttatcgct aatcatggaa agtatgaatg gctttccata
     3541 acgacgccct agcagaaaaa tgataatcga accaatgcag attccaatat agttatagat
     3601 aaatccagca taagggccaa atatcaggac accagctgca gtactgatcc ctccaggaat
     3661 gattggaata actacttgca ggatttggat gaagacaaaa ataatcggtc ctaaaatgat
     3721 ggaatcacct actaatcctc ttaacgcatt caaatctttg aaaacaccta aattaataaa
     3781 ataaatcgtc agcgcgattg ataggcctaa tccgataatg gatatgaaat ttatcagttt
     3841 tcgagaggca gcaatattca ttattcatca actccttaga ggttctttgt taactatact
     3901 ttactagaaa agcaactaac tgcgccattt tttgatatga cagaaaacaa acaattttgt
     3961 aagttcatca taagtataaa agattgatct caaaaaaaca tccaactaaa gacttattta
     4021 ataacttgtt
//
LOCUS       DAGCYL010000196         3898 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.199, whole genome shotgun sequence.
ACCESSION   DAGCYL010000196 DAGCYL010000000
VERSION     DAGCYL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.199"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            50..889
                     /locus_tag="MB216_002521"
     CDS             50..889
                     /locus_tag="MB216_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4527303.1"
                     /translation="MPRKPLSEEDKKARSIIANNLKKITHGYTRAQLSDMTGIPVTTL
                     SGYFTEKSTISMENAELIAKAFNVDKSDIDPRYSREYNYYDLKKEVLDELQENNKSFV
                     ADKFVELAEKANAAGMTLNRTKTDRGNIMTVLSSDIQEYNFFDTSVAAGIPSSVEAFD
                     QDHIEQIAIPDYAMGKYAGYQDVFFTKINGDSMNNVIPDGSLIAVKKIESCDELKDGD
                     IVVFSNDNEFSVKRYINDQQNNRIIFRPDSSNISFTDIVVNYDDTKYVQIYGKVVVYI
                     VQL"
     gene            910..1110
                     /locus_tag="MB216_002522"
     CDS             910..1110
                     /locus_tag="MB216_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527304.1"
                     /translation="MKIGMRKPSIKKSIGARTTGKAKRAVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            1196..1636
                     /locus_tag="MB216_002523"
     CDS             1196..1636
                     /locus_tag="MB216_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527305.1"
                     /translation="MKFIKFGIIGLLVVTVMGGCGTSTAETKESKNYSETNSTIKEVD
                     SDQTAEFATIAEKHIKEIYSIDNLKMDLKSIKVTQFPDDKNAETGEEYKNVYNGTGDF
                     TWQDKKYNFSLIYSKKDATNYTVLYLYSNLDPNKGVDTPLESDQ"
     gene            1775..2980
                     /locus_tag="MB216_002524"
     CDS             1775..2980
                     /locus_tag="MB216_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4527306.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIEFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQSENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(3032..3119)
                     /locus_tag="MB216_002525"
     tRNA            complement(3032..3119)
                     /locus_tag="MB216_002525"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3085..3087),aa:Ser,seq:cga)
     gene            complement(3264..3830)
                     /locus_tag="MB216_002526"
     CDS             complement(3264..3830)
                     /locus_tag="MB216_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4527307.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT         1452 a          628 c          661 g         1157 t
ORIGIN      
        1 tatattgatt taattcgata ttattaataa aaaaggagga gaaaataaca tgccaagaaa
       61 accattatca gaagaagata aaaaagctcg ctccattatc gctaacaatt taaaaaaaat
      121 tacgcatggc tacacaagag cacaattgtc agatatgaca ggtattcctg ttacgacgct
      181 atcgggatat tttactgaaa aatcaactat cagtatggaa aacgctgaat taatagctaa
      241 agcttttaat gttgataaat ctgatataga tccaagatat tccagagaat acaattatta
      301 tgatttaaag aaagaagttt tagatgaatt acaggaaaac aataaaagtt tcgttgctga
      361 taaatttgta gagttagcag aaaaagcaaa tgcagcaggg atgactttga acagaactaa
      421 aactgataga ggaaacatta tgacagttct atcaagtgat attcaagaat ataacttttt
      481 tgacacttct gttgctgccg gcataccttc ttcagtcgaa gcttttgatc aagaccatat
      541 tgagcaaatt gctattcctg attatgccat gggaaaatat gcaggatatc aagatgtatt
      601 ttttactaaa ataaatggag attccatgaa taacgttatt cctgatggat cattaattgc
      661 tgtaaagaaa atagagagct gtgatgagtt aaaagatgga gatatagtag tctttagcaa
      721 cgataacgaa ttttctgtaa aaagatatat aaatgatcaa caaaataatc gcatcatttt
      781 tagacctgat tcttctaaca tttctttcac tgatattgtg gttaattatg atgatactaa
      841 atacgttcaa atttatggga aagtcgttgt atatattgtc caactataat taatttgaga
      901 gggtataaaa tgaaaattgg aatgagaaaa ccgagcataa agaaatctat cggcgctaga
      961 actactggaa aggcaaaaag agctgtgaaa aaagctgtga ttcctggtta tggaaagaaa
     1021 ggctctggat ggattaaaga cccaaaaaaa gctgcataca ataaagttta taaaaagact
     1081 acgtttagct tttgggactt atttaaataa aaaaagcccg tgcagcaaca cggactcgaa
     1141 accttatttc taagattccc caataaaata atatcataga gataaggagt agaaaatgaa
     1201 atttattaaa tttggaatta ttggattact agtagtgaca gttatgggag gatgtggaac
     1261 atcaacagct gaaacaaagg aatcaaaaaa ttatagtgag acaaacagta ctatcaaaga
     1321 agtagactct gatcaaaccg ctgagtttgc aactattgcc gagaaacaca ttaaagaaat
     1381 atattcaata gataatttga agatggattt aaaatctatc aaagttactc aatttcctga
     1441 cgataaaaat gcagaaactg gtgaagagta caaaaatgtc tacaatggta ctggagattt
     1501 tacttggcaa gacaaaaaat acaacttttc tttgatttat tcgaaaaaag atgcaactaa
     1561 ttataccgtc ctatatctat atagcaatct agatcctaat aaaggtgtag atacaccatt
     1621 agaaagcgat caataaaaaa agcacattta ccgcccgacc aagaacgcaa atgtgcaaag
     1681 agctacggta aagtaggtct atttgttgta ccctatttta ccatctcgaa atccgctgta
     1741 caaccgaaca aacgtacgaa aggaaagata aaagatggca aaaaaagaaa ttgataaacg
     1801 aattaaaaag cacttaacaa aaaaaggaga agaaaaatat cagtttagtc tgtacttagg
     1861 agttgatccg ctaacaggaa aaaaaagaaa aacaacgaga cgaggtttta gtacgccatt
     1921 agccgctgag agagctttaa aaagattgga ggcagatata caggaaaaag gactgcaagc
     1981 ttctacagct caaaagagca agaaattcga atatgtttac ggcttatggt ttgaaaatta
     2041 taaaaagaca gttaaagaaa gcacttggtc ctccaccaaa caaatttttg acacacacat
     2101 cttaaaagtg tttggagata agttcattga caaaatagac gtttttttct gtcaagaagc
     2161 agtaaacact tggtccgata gccatcctaa aatttttaag aaaataaaaa attacacatc
     2221 taatgttttt gattacgctg cttctttaca aattataact agcaatccta tgaaattagt
     2281 ttctattcca cgtggagaag ctctagatat cgaagataaa aatatcgaat tttatactaa
     2341 agaagaatta atcgaatttt tagaagcaat aagaaacgat gatgatgaaa gatatttatt
     2401 cttttcccta ttggctttta caggtataag aaaaggagaa gcttttgcac tgacctggtc
     2461 ggatattgat tttaagagca aaacactcaa tattaataaa actgtaacaa gaggttatca
     2521 agggagattg atcgtaaata ctcctaaatc aaaatctgga aaaagaaaaa tttatctgga
     2581 caatgatttg atcagtttat tgagaaagta ttatacaaaa aacaaaacga tcgttacaat
     2641 ccaaagcgaa aacttaattt ttcaccatga tggtcttctc tataatccta cagtttcacg
     2701 ttcgtggctt aatgtcatat ataagcatca tccagaatta actaaaagaa tcactactca
     2761 tggttttcga catactcatg cctctctcct ttttgaatca ggagcttctt taaaagatgt
     2821 tcaggaacgt ttagggcatg cagacattca aactacttca aatatttaca ctcatgtaac
     2881 tgaaactcaa aacaaaaaag tcatcaataa ttttgtagca tttatgaaga atagtactca
     2941 gggggagtca aaaggggagt caaaaaacat tttaggctaa attacaacaa aaaaagacac
     3001 gatcgaaatc gtgtctaatc gttgatatat aaggaaggta caggatttga acctgcgcgc
     3061 cggtattagc cggttcgccg gatttcgagt ccggtgcatt accactctgc caaccttccg
     3121 taagtactat ttttaaactt ttttcacgaa atgtcaagcc tatttctaat agataaataa
     3181 cagaaatcgc tttttttagc aaaaaagtcc attgacagtt tgcagccaac ggacttaccg
     3241 atcaaattca tactgataga acactatctg attatttcag gattatctaa tgctaaaatc
     3301 gagtcaattg tccgtttgat cagtgcaatg atttgttctt ctgttggatg catgatgttt
     3361 gatgacatca atgacgctaa gccagtcact gctacccatg taccagtatg cagagcatcg
     3421 acatattcat ccgataattg attgtatctt tcatcttctt taactatttc acggaaataa
     3481 ttgtatgaaa aatcatgcat tttctttccg tccccatgtt catcaatgta caatgccatg
     3541 taaagactat gatgcttttt tgcaaagtag atatagttca aaccgagatc aatgatttta
     3601 tcaccagtac gttcaatagg gaaaacatct tcttttagtt gttcaaagat agaatccagc
     3661 aacgtatttt ttaaatcttc catgtttttg aattccagat aaatcggctg tgtcgaaatc
     3721 cccatctttt tagcaatatt tctcgcagta aatcctttaa acccttcctt tgcaacaaca
     3781 tcataagcag cttttagaat ttgatccttg gtatagactt ttcttctcat taaaactccc
     3841 ccacttcttt actgcagtat atgttgtttt attgtaccct atttttagca ttttttgc
//
LOCUS       DAGCYL010000197         3785 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.200, whole genome shotgun sequence.
ACCESSION   DAGCYL010000197 DAGCYL010000000
VERSION     DAGCYL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.200"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..171
                     /locus_tag="MB216_002527"
     CDS             <1..171
                     /locus_tag="MB216_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBT4527308.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            406..642
                     /locus_tag="MB216_002528"
     CDS             406..642
                     /locus_tag="MB216_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527309.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="MB216_002529"
     CDS             649..2601
                     /locus_tag="MB216_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBT4527310.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="MB216_002530"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="MB216_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBT4527311.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3400..>3785
                     /locus_tag="MB216_002531"
     CDS             3400..>3785
                     /locus_tag="MB216_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBT4527312.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DAGCYL010000198         3735 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.201, whole genome shotgun sequence.
ACCESSION   DAGCYL010000198 DAGCYL010000000
VERSION     DAGCYL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.201"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            159..473
                     /locus_tag="MB216_002532"
     CDS             159..473
                     /locus_tag="MB216_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4527313.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            489..875
                     /locus_tag="MB216_002533"
     CDS             489..875
                     /locus_tag="MB216_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4527314.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            904..2289
                     /locus_tag="MB216_002534"
     CDS             904..2289
                     /locus_tag="MB216_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBT4527315.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2292..2445
                     /locus_tag="MB216_002535"
                     /pseudo
     CDS             2292..2445
                     /locus_tag="MB216_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            2468..3673
                     /locus_tag="MB216_002536"
     CDS             2468..3673
                     /locus_tag="MB216_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBT4527316.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         1226 a          684 c          815 g         1010 t
ORIGIN      
        1 gtagacactt caaaaaatga ggtgtctatt tttttacccg attttgaaag gaagtgaact
       61 tatgaaaaca aaaaatcaag aatcaaaagg tcgttcccca ctctttaaga ccatcaaaca
      121 ttcattcagc caataaaaaa gaaaggatag gtaaaaatat ggaacttaaa tttgtgattc
      181 ccaacatgga aaaaacattc ggcaatttag aatttgctgg cgaggataaa gtcgttcagc
      241 gaagaatcaa cggacggcta actgtcttat caagaagcta taatctctat tctgatgttc
      301 aaagagcaga tgatattgtg gtggtgcttc ctgctgaagc tggcgaaaaa catttcggct
      361 ttgaggaacg tgtgaagtta gtcaatccac gtattaccgc agagggctac aaaatcggca
      421 ctcgtggttt tacaaattac cttttacatg ctgacgacat gataaaagaa taaagaaaga
      481 gaggaaaaat gatgagatta gcaaatggca ttgtattaga taaagacacg acttttggag
      541 aattgaaatt ctctgctcta cgtcgtgaag tgagaatcca aaatgaagac gggtcggttt
      601 cagatgaaat caaggaacgt acctatgact taaaatccaa aggacaagga cgcatgattc
      661 aagtaagtat tcctgccagc gtgcctttga aagagtttga ttataacgca cgggtggaac
      721 ttatcaatcc cattgcggac accgttgcta ctgccaccta tcaaggagca gatgttgact
      781 ggtatatcaa ggcagacgat attgtgctga caaaggattc tagttcattc aaagctcaac
      841 cacaagcaaa gaaagaaccg acacaagaca aatagtcgct aggtagaaag gagacttttt
      901 cgcatgaaac agcgtggtaa aaggattcgc ccatctggta aagatttagt ctttcatttt
      961 acgatagcgt cactcctgcc tgttttcctg ctggttgtcg gactgtttca tgtgaagaca
     1021 atccagcaga tcaactggca ggattttaac ctatcacaag cagataagat tgacattccc
     1081 tatttaatta tcagtttcag tgtcgcaatt cttatctgct tgctggtagc gtttgtattc
     1141 aaacgggttc gctatgatac ggttaaacaa ctttaccacc gtcaaaaact ggcaaagatg
     1201 atacttgaaa acaagtggta tgaatctgaa caggtcaaaa cagagggttt ctttaaagat
     1261 agtgctggtc gtacaaagga aaagataacc tacttcccta aaatgtatta tcgacttaaa
     1321 aatggcttga tacagatacg ggtggaaatc acgctgggaa aatatcaaga ccaactctta
     1381 cacttggaaa agaaattaga gagtggcttg tactgtgagc tgacggataa agagttaaag
     1441 gattcctatg tggaatatac tttgctctat gacaccatag ccagtcgtat ttctattgat
     1501 gaagtagaag ctaaagatgg taaacttcgc ttaatgaaaa acgtatggtg ggaatatgat
     1561 aagctccctc atatgttgat tgctggtggt acaggtggcg gtaaaactta ctttatactg
     1621 acactgattg aagccttgct tcatacagat tcaaaactgt atattcttga cccgaaaaat
     1681 gctgaccttg cggacttagg ttctgtgatg gcaaatgtct actatagaaa agaagacttg
     1741 ctttcttgca ttgaaacatt ctatgaagaa atgatgaaac gtagtgagga aatgaagcag
     1801 atgaagaact ataagactgg caaaaattat gcttacttag gtctcccggc acacttctta
     1861 atctttgatg aatacgtcgc tttcatggaa atgctgggaa caaaagaaaa caccgcagtt
     1921 atgaataagc tgaaacagat tgtcatgtta ggtcgtcaag ctggcttctt tctaatactg
     1981 gcttgtcaac gtccagacgc aaaatattta ggcgacggaa tccgtgatca gtttaatttc
     2041 agagtggctt taggtcgtat gtctgaaatg ggctatggca tgatgtttgg cagtgacgta
     2101 caaaaggatt tcttcttaaa gcgaatcaaa ggtcgtggct atgttgatgt aggaacaagt
     2161 gtcatatcag agttttatac tccccttgta ccaaaaggat atgatttctt ggaggaaatt
     2221 aaaaagttat ccaacagcag acagtccacg caggcgacgt gcgaagcgga agtcgcaggt
     2281 gtggactgat cttgctggct ggtgtggcaa tagccacgcc agcacttaac ccctcggtat
     2341 ctaacagggg ggtacaaatc gacaggaaac agtcaaaaaa acattagaaa atcctttggt
     2401 tacaagggat ttacaaaatt tcagcgtatg tcaaatgggc tttaaaagtt gacatacgcc
     2461 tttttgattg gagggatttt tactgaatga acaaacttgg ttacagcatt taaaagaaaa
     2521 acgcttggct tatggactat ctcaaaaccg tttagctgtt gcgactggta ttacaaggca
     2581 gtatctaagc gatattgaaa caggaaaagt caagccatca gaggatttac agcagtccct
     2641 ttgggaagct ctggaacgct tcaatcccga cgctcccctt gaaatgctgt ttgattatgt
     2701 aagaattcgc tttccgacaa cagacgtaca gcaggtggtc gaaaacatct tacaactgaa
     2761 actgtcctat tttcttcatg aggactatgg tttctattct tattcagagc attatgcttt
     2821 aggcgacata ttcgtccttt gctcccatga actggacaaa ggagttctgg tggaattgaa
     2881 aggtcgtggg tgcagacaat ttgaaagcta tcttctggca caacaaagaa gctggtatga
     2941 gttctttatg gacgttttgg tggctggcgg tgtgatgaaa cgccttgacc ttgccattaa
     3001 cgataagaca gggattttaa atatccctgt actcactgaa aagtgccaac aggaagaatg
     3061 tatctccgtc ttccgcagtt ttaaaagcta tcgcagtggc gaactggtac gcaaagagga
     3121 aaaggaatgt atgggaaaca ccctctatat cggttcatta caaagtgaag tttatttctg
     3181 tatctatgaa aaggactacg agcagtacaa gaaaaatgat attcccattg aagacgcaga
     3241 agtaaaaaac cgttttgaga ttcgattgaa aaatgagcgt gcctattatg cagtccgtga
     3301 tttactcgtc tatgacaatc cagagcatac cgcctttaaa attatcaatc ggtatatccg
     3361 ttttgtagat aaagacgatt ccaaacctcg ttctgattgg aaactgaatg aagaatgggc
     3421 ttggtttatt gggaacaatc gtgaacgatt aaaactaacc acaaaaccag agccttactc
     3481 cttccaaagg acgctgaact ggctatctca tcaagttgcc ccgaccttaa aggttgcgat
     3541 taaacttgat gaaatcaacc agacgcaggt tgtaaaagac attctcgacc atgcgaaact
     3601 gacagaccga cacaagcaga ttttgaagca acagtcagta aaagaacagg acgtgataac
     3661 aacaaaaaaa taactcaaat acaaattcat tgaatataga gaggagaaca tttttatgaa
     3721 ttttggacaa aacct
//
LOCUS       DAGCYL010000199         3725 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.202, whole genome shotgun sequence.
ACCESSION   DAGCYL010000199 DAGCYL010000000
VERSION     DAGCYL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.202"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            109..2550
                     /locus_tag="MB216_002537"
     CDS             109..2550
                     /locus_tag="MB216_002537"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBT4527317.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2880..3584)
                     /locus_tag="MB216_002538"
     CDS             complement(2880..3584)
                     /locus_tag="MB216_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527318.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1197 a          654 c          815 g         1059 t
ORIGIN      
        1 gagaatattt aaggattgtt aaaatttaat cttagaaaaa aataactgga acaagagcta
       61 tttgttttta cagcaaagaa tggtatattt ttgtaggagg tggcttactt gaaagaagtc
      121 gttatgatag atgctgcaag aacgccgata ggaaagtatc gcggaagttt aagtccattt
      181 actgcagttg aattaggcac acttgtgact aaaggattat tagataaaac aaaactgaaa
      241 aaagataaaa tcgatcaagt aatatttgga aatgtcctgc aagctggtaa tggtcaaaat
      301 gttgccagac agattgcttt gaacagcggt ctccctgtag atgtcccagc aatgacgatc
      361 aatgaagttt gcggatctgg tatgaaagca gtgatcttag cacgccagct gatccaatta
      421 ggtgaagcag aacttgtgat agccggcgga acagagagta tgtctcaagc acctatgctg
      481 aaacagtatc agtcagaaac aaatgaatat ggtgaaccaa tttccagtat ggtcaacgac
      541 ggattgactg acgcattttc aaatgcacat atgggattaa ccgcagagaa tgttgcaaca
      601 caattttctg tgagcagaga agagcaggat cgctatgcct tgtcgtccca gttgaaagca
      661 gcacatgctg tcgaagccgg tgtattttct gaggagatca tcccagtcaa gatttctgat
      721 gaagacgtgt tatctgagga tgaagcagtt cgtggaaata gtacattgga aaaactgggc
      781 acgttacgta cagtattctc agaagaagga actgtaacag caggaaatgc ttccccgttg
      841 aatgacggtg cctctgtggt gatccttgca tccaaagaat acgcagaaaa taataatctg
      901 ccttatttag caaccatcaa agaagtagcg gaggtcggta ttgacccaag tatcatggga
      961 atcgctccaa tcaaagccat tcaaaaattg acagatagat caggaatgaa tttatcaaca
     1021 atcgatcttt ttgagatcaa tgaagcattt gcagcttctt ctatcgtagt tagccaagaa
     1081 ctgcagttag atgaagaaaa ggtcaatata tatggaggag caatcgcatt aggccatcca
     1141 attggtgcaa gcggtgcacg tatcttgact acattagctt acggattgct tcgtgaacag
     1201 aagagatacg gtattgcttc attatgtatt ggtggaggtt taggcttggc agtcttgtta
     1261 gaagcgaata tggagcaaac acataaggat gtgcaaaaaa aaaagtttta tcagcttacg
     1321 ccatctgaac ggcgcagtca attaatagaa aaaaatgtat tgacacaaga aactgcgttg
     1381 atttttcagg aacaaacact ttctgaagag ctctccgatc acatgatcga aaaccaagtg
     1441 agtgaagtgg aaatcccaat ggggatcgcg cagaattttc aaatcaatgg caagaaaaaa
     1501 tggattccga tggcaacaga ggaaccatct gtcattgcag ctgcaagtaa tggtgcaaaa
     1561 atctgtggaa atatctgcgc agaaacacct cagcggctga tgcgagggca aatcgttcta
     1621 tcaggaaaat cggagtatca agcagtgatc aatgcagtta atcatcgaaa agaagagctg
     1681 attctttgtg caaatgaaag ttatccttca atcgtgaaac gcgggggcgg tgttcaagat
     1741 atctctactc gggaattcat ggggagtttc catgcatact tatctattga ttttttagtg
     1801 gatgtaaaag atgcaatggg cgctaacatg atcaactcga ttcttgagag tgtggcaaac
     1861 aagttacggg agtggttccc tgaagaagag attctattca gtattttaag taattttgct
     1921 acagaatcat tggcttctgc ctgttgtgaa atcccattcg aacgcttagg gagaaacaaa
     1981 gaaatcggtg aacaaattgc caaaaaaatc caacaggcag gggaatatgc gaagcttgac
     2041 ccttaccgtg cagcaacgca caacaaaggg atcatgaatg gtatagaagc tgtagtggca
     2101 gctacaggaa atgatacacg tgcggttagc gcttcgatcc acgcctacgc ggcaagaaat
     2161 ggattatacc aaggattaac agactggcag atcaaagggg ataaactagt tggaaaacta
     2221 acggtgcctt tggctgttgc cacagtaggc ggagcttcca atatcctgcc aaaagcaaaa
     2281 gcatctttag ctatgttaga tattgattca gccaaggaac tagcacaagt aattgctgca
     2341 gtagggttag ctcaaaacct ggctgctttg cgtgcattag ttacagaagg aatccaaaaa
     2401 ggccacatgg gacttcaagc tcgttcatta gcgatctcaa ttggggctat tggtgaggaa
     2461 atcgagcaag ttgcgaaaaa attacgtgaa gcagaaaaga tgaaccagca gacagctata
     2521 caaatattgg aaaaaatccg ggaaaaataa aaaagcaata tctttagaag aataaagcaa
     2581 gatttacagt tattttattt actgcagatc ttgctttttt ctctatcaga aaatatgttt
     2641 attattagaa ttttttatag ttaaggttag gcatcttaag aaatcaggca actccaagat
     2701 atcttcaggt agaagagaag tcgcctaaca ttgtaggatc ataggtgatt tgttggacag
     2761 gtcaagctga tatttgagcc tgatgttaga aggaatttct ttttacttgc tgtagaaatt
     2821 attatatgac taaaaaggct gggattttaa atcccagcct tttatgacaa atagaatttt
     2881 tatcgatcag taatcgctgt cgctgcaaat tgttcgatca aaggaatcaa agtcaatttg
     2941 actttgtatt tccgttcgag tgctttttcg ataacgaact cattttcagt agtcatagaa
     3001 atagttgcta atttacgagg ttcatcagca tttttttcat taataactac ttcatgttca
     3061 ttttgttctg tgtaaaattt aggatactca gcaacggctt ttgaaaaatc agcaaaactt
     3121 aaagcggcat tcgtatcagt tgagcgtgta ttttcagttg tgtccgcttt tggtttttcc
     3181 gttttttctt ctggcacact gactgtttct tctgtaactg gtgtcgcaac agcttgcgca
     3241 gttggttcat cgtttttcaa tcgttgatca ttccagtaag acacgatttc aataagaaga
     3301 ttgtcacgga atcgttcgaa aatcccgttt tctcctgaac gaattctctt taatacagta
     3361 ttgaagttgt catcgattgg cacgttgatc cgtgtatctt catttgtttt cccagctaat
     3421 ccgatcgtta agatttgacg gtttttggga tcaagcatga cttgtaacgg caaaccataa
     3481 cgtcgcaagc tctcttcttc tagagaacgg aataattgtc ctcttacacc atccaatgaa
     3541 ggacggaatt gatttaataa tttttgtttc tgttttttct tcatatgttc tctccttcgc
     3601 tcataccctt tcgattataa gaaaaaaaca gccaaatgaa aaggagtaat gcaaaaaaac
     3661 ttgcgtatac gcaaattaca acagaaaaat ttgcgtattt tcccgttttt tgaaagaaaa
     3721 ataaa
//
LOCUS       DAGCYL010000200         3605 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.203, whole genome shotgun sequence.
ACCESSION   DAGCYL010000200 DAGCYL010000000
VERSION     DAGCYL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.203"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            56..508
                     /locus_tag="MB216_002539"
     CDS             56..508
                     /locus_tag="MB216_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527319.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            522..1211
                     /locus_tag="MB216_002540"
     CDS             522..1211
                     /locus_tag="MB216_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527320.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1336..>3605
                     /locus_tag="MB216_002541"
     CDS             1336..>3605
                     /locus_tag="MB216_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBT4527321.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLD"
BASE COUNT         1220 a          473 c          753 g         1159 t
ORIGIN      
        1 attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga gaatcgtgaa
       61 aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct attttctttt
      121 gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag ctgaacataa
      181 taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag aattgtataa
      241 agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa ttagtgactc
      301 agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca aatccgaatt
      361 agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt ccaacgctat
      421 ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa agcagataaa
      481 agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag atttttggtg
      541 tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct gctgagaaag
      601 aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt gaagaagaag
      661 ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa attgtatcta
      721 tggataatcc agatataatt caaggttcat ataattcaga aactgatcct gcttcagttt
      781 cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt gaaactactc
      841 caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac cttcttgtac
      901 tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg cgttttggag
      961 gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa tcctacattg
     1021 acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt tctattcaag
     1081 gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg cctggttgga
     1141 caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat tatgttgttc
     1201 aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta tttttagttg
     1261 ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg ttcggcaaga
     1321 aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct aaacaagaaa
     1381 tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg gtggcctaca
     1441 atctatggac ttattgaagg actacaagta gaacttggta ttactaatct agtagctaat
     1501 tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg gggcaaaaat
     1561 ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg gattaatcca
     1621 ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga gttgcagact
     1681 gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg ggccaaagcg
     1741 ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat tagaaaaatg
     1801 cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg tgatggtatt
     1861 tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact aggatattct
     1921 cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta tcctgtgtct
     1981 gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt taatggttat
     2041 tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga agtactggca
     2101 ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt tgtaataaaa
     2161 ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga tacttctacg
     2221 caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat agttggtaga
     2281 tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct aactacagag
     2341 gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca agatggaggt
     2401 gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa ggctatagat
     2461 gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt tgatctagac
     2521 atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt atctgctgta
     2581 atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg ttctcaaata
     2641 atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg ttatagtggg
     2701 aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga atttaccata
     2761 ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag agatagcgga
     2821 tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata caatcagaag
     2881 gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct tgataatcct
     2941 tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc atgggacatc
     3001 atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag gaataaatta
     3061 actaatagcg agggcctgta taattatttt atagatccta atactggttc aataataggt
     3121 ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac aatcaatgat
     3181 agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg tgaagttaag
     3241 aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc tgcaggtgga
     3301 acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta taccttagca
     3361 aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg tgataagggt
     3421 gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga taaaatcgct
     3481 tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg tgtagggata
     3541 atttcatttt tcattgataa aatagttaat aattcacttg ggacagattt tacttatctt
     3601 gattt
//
LOCUS       DAGCYL010000201         3583 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.205, whole genome shotgun sequence.
ACCESSION   DAGCYL010000201 DAGCYL010000000
VERSION     DAGCYL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.205"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            258..1040
                     /gene="rpsB"
                     /locus_tag="MB216_002542"
     CDS             258..1040
                     /gene="rpsB"
                     /locus_tag="MB216_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBT4527322.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1139..2020
                     /locus_tag="MB216_002543"
     CDS             1139..2020
                     /locus_tag="MB216_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBT4527323.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2156..2878
                     /locus_tag="MB216_002544"
     CDS             2156..2878
                     /locus_tag="MB216_002544"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBT4527324.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            2881..3438
                     /gene="frr"
                     /locus_tag="MB216_002545"
     CDS             2881..3438
                     /gene="frr"
                     /locus_tag="MB216_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBT4527325.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         1324 a          611 c          784 g          864 t
ORIGIN      
        1 aaggataata aagaaacaaa gagaaatcta ctgtttttct tgaattcttt ttatctttca
       61 ggtaaactat tgaatgttga gcagaaaagt atatttgctc ttgcgatacg gatcttttgt
      121 ctgtataata gctgagggtg ctttatgcat cagcaaatcc acattcttat tggattgtcg
      181 gtaggtgctt gaaaaagttt gccggcaagg tagaagataa gaagaggaaa aataaaacca
      241 aatcggagga aacaaacatg gcagtaattt ctatgaaaca attactagaa gccggcgtac
      301 actttggtca ccaaactcgt cgctggaatc caaaaatgaa gaaatatatc ttcacagaaa
      361 gaaacggaat ctacatcatt gacttgcaaa aaacagttaa attagtagat gcagcttatg
      421 attacatgaa gagcgttgct gaagaaggcg gcgtagcatt attcgtaggt acaaaaaaac
      481 aagcacaaga agcaatcaaa gaagaagcaa ctcgtgcagg acaatacttc gttaaccatc
      541 gttggttagg cggaacattg acaaactggg atactatcca aaaacgtatc agccgtttga
      601 aacaaatcaa tgcaatggaa gaagatggaa cttttgaagt tctacctaaa aaagaagttg
      661 ctggtttgaa caaagaacgt gaacgtcttg aaaaattctt aggcggtatc gctgatatgc
      721 caagaatccc agatgtaatg tacatcgttg acccacgtaa agaacgtatt gctgttcaag
      781 aagcacacaa attaaatatc ccaatcgtag ctatggttga tacaaactgc gatccagatg
      841 aaatcgacgt agtaatccct tcaaacgacg atgcaattcg tgccgttaaa ttgattactt
      901 caaaaatggc tgatgctttc atcgaaggaa accaaggtga agatcaagta gttgaagaag
      961 atttcacaga agaaaacaca gcaacatcaa tcgaagaaat cgtagatgtt gttgaaggcg
     1021 acaattcttc agcagaataa ttttgaatga tggagctgtt tcaaaggctg ggcgacaagc
     1081 ctcactctcc tttgaagcag ctttttttaa aaagaaaaac acacaggagg agttaaacat
     1141 ggcagacgtt acagctaaaa tggtaaaaga actacgcgaa atgactggcg taggaatgat
     1201 ggatgcgaaa aaagcattag ttgaagtaga aggcgacatg gaaaaagcag ttgatctttt
     1261 acgtgaaaaa ggaatggcaa aggctgcgaa gaaaaatgac cgcattgcag ctgaaggatt
     1321 ggcttcagtt gctgtgaaag gtaacactgc agcaatcgtt gaagtaaact cagaaacaga
     1381 ctttgtttct aaaaacgaaa tgttccaaga tttagtaaaa gaaattgcag aacttgttgc
     1441 tgaaaacaaa ccagcagaca tggaagctgc aatgaaaatc aaaacagata aaggtacaat
     1501 tgaatctgac ttgatcgaag caactcaagt tatcggagaa aaaatcagct tccgtcgctt
     1561 tgaagttgta gaaaaagacg acaatgctgc atttggtgga tacttgcata tgggcggacg
     1621 tattgccgta ttgactgttc tagatggaac aactgacgaa acagttgcaa gagacgtagc
     1681 tatgcacgta gcagctatca atcctcgtta tgtaaacgaa tcacaaattc cagaagctga
     1741 attagaacat gaaaaaactg ttcttacaga acaagcattg aacgaaggca aaccagcgaa
     1801 tatcgttgaa aaaatggtag aaggacgctt gaagaaattc aaagcagaaa tcgctttggt
     1861 ggaccaacca ttcgttaaag atcctgatat gactgttgaa aaatacgttg catcaaaagg
     1921 cgcaactgtt aaaacattcg tacgtttcga agttggcgaa ggtatcgaaa aacgcgaaga
     1981 taactttgta gaagaagtta tgagccaagt gaaaaaataa ttctgttgaa taacagacgg
     2041 gaacgcacgg tttgtgcgct cccttttttt aagcaaaaca agaaaaacca gtcttagagt
     2101 gtcgaaatca tctaagtatg gtaaaatggc aatagaaaat attggaggaa tgaacatggt
     2161 cacaccgaaa tatcagcgtg tagtcttaaa attgagtgga gaagctttag ctggggacgc
     2221 aggatttgga atcaaaccac cagtaatcaa agaaatcatc caagaaatca aagaagttca
     2281 cgagctgggc gttgaaatgg caatcgttgt gggcggcgga aatatctggc gcggacaaat
     2341 cggagcacaa atgggcatgg aacgtgctca agctgattac atgggtatgt tagctacagt
     2401 tatgaatgcc ttagctttac aggatacgtt ggaaaacgtg ggtgtaccga cacgcgtgca
     2461 aacttcgatt gaaatgcgtc aaatcgcaga gccatatatt cgccgtaaag cagaacgtca
     2521 tttagaaaaa ggacggatcg ttatctttgc aggcggtacc ggcaacccgt acttctcgac
     2581 agacacaaca gcagcattac gcgcggcaga aatcggcgca gatgttatcc tgatggctaa
     2641 aaacaatgta gacggtgttt attctgctga tccgaaagta gatgcaaatg cagtaaaatt
     2701 tgaagaactg actcatctgg aagttattgc caaaggatta caagtcatgg attcaactgc
     2761 aagttcacta agcatggata atgatattcc actgcttgtc ttcaatttaa atgaacacgg
     2821 aaatattcgc cgtgcaatct taggcgaaaa tatcggaaca actgtaaggg ggaaataaaa
     2881 atggcagatg caattatgac agaagcaaaa gaaaaaatga aaaaagcagc acaaaatctt
     2941 caacgtgaac ttgggcaaat ccgcgcaggc cgtgcgaacg ctagcctttt ggaccgtatt
     3001 actgtgaact actacggtgc gccaacacca ttgaaccaaa tggcatcaat ccaaattcca
     3061 gaagcacgtg ttttaatgat cacacctttt gacaagagca ttcttcaaga tgttgaaaaa
     3121 gctattatgg caagtgatat cggaatcagt ccaaccaatg atggaaatgt cattcgctta
     3181 gttatcccgc aactgacaga agaacgcaga aaagaattag ctaaagatgt gaaaaaagaa
     3241 gcagaaaatg caaaaatcgc gattcgtaac atccgtcgtg atgcgatcga tgaatataaa
     3301 aaacaacaaa aaaatggtga tattaccgaa gatgatttgc gcggattaga aaaagacgta
     3361 caaacattga cagataacag catcaaagaa gtagataaga tcgctgctga taaagaaaaa
     3421 gaattattgg aagtttaata aaatcataaa aacagagggg cggatcattc cgcttcctct
     3481 gtttttattt atatttcttt tatttaaaaa ttaaaaaaag aaaaaattat tcagttttta
     3541 aataaaaaaa caatgatttc ataggaaaaa cagttagata ttg
//
LOCUS       DAGCYL010000202         3552 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.206, whole genome shotgun sequence.
ACCESSION   DAGCYL010000202 DAGCYL010000000
VERSION     DAGCYL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.206"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(175..852)
                     /gene="nth"
                     /locus_tag="MB216_002546"
     CDS             complement(175..852)
                     /gene="nth"
                     /locus_tag="MB216_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBT4527326.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(859..1557)
                     /locus_tag="MB216_002547"
     CDS             complement(859..1557)
                     /locus_tag="MB216_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBT4527327.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1825..3009
                     /locus_tag="MB216_002548"
     CDS             1825..3009
                     /locus_tag="MB216_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBT4527328.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3015..3458)
                     /locus_tag="MB216_002549"
     CDS             complement(3015..3458)
                     /locus_tag="MB216_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4527329.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          914 a          716 c          594 g         1328 t
ORIGIN      
        1 ttttcccaat aaaaataaca acttttattt ttatcgcaat aaattgtaat aagttaccat
       61 cctagctact gagtatgtac atttactttt tcatgcaaaa aagataaacg aaacagcttt
      121 tctccataat aacaagataa ttcatacatt tatttgcaat taaagaaacc acgtctacag
      181 gcgtggtttc ttttttagcg ccttttcttt tagacgcatc cgatttttcc catcttgaca
      241 tattgatagc aaaggacata cctcgcactt gggatttctt gccgtgcaat gatatctacc
      301 aaaaaaaatc agtgtatgat gtgtctttac ccataattct tgtggtactt tccgcatcag
      361 tgtttcttct acttccatga ccgtagcatc tagtttgcag atcctcaggc gttttgagac
      421 acgttccacg tgggtatcta ctgcgattgc tggtatcccg aatgcatccc ccaatacgac
      481 atttgctgtc tttctgccta caccaggaag agacatcaat tcttctcttg acgttggcac
      541 ttgcccatcg aaacgttcaa tcagctgctg tgcacacgct ttgatgtttt ttgctttatt
      601 tcgatacaaa ccaatcgttt ttattttaag aatgatttca tcgattgaag cttctgctaa
      661 cgcatcgggt gtcggaaaag aggcaaacaa atctggcgtt gctttattca ctgatacatc
      721 tgtcgcctgg gcacttaaga tcactgcaat caataactca aatggattat tgtgcttgag
      781 ttctccatgt gcttcgggaa acattccata catcgtttct agagcttcca ttgttttttg
      841 tttgcttaac atattccgct actcctccaa ccaatttttc agtgagactt tcggcaattc
      901 ttcttgttga gctgactgtt ctacttctct ttgaagaagc tgctgttttc ttcgtttttg
      961 gtcttcttcc acttgttgtt ttgtacgtag attttttctt tcccacgata gcaaaatgcg
     1021 gtcaacgtaa ttaaaactat aagcctgatt taatacggct tcccgcagtg ctagcttcaa
     1081 aatttccggc tgataatggt cttcttctag ccattgtccg atccgctgga actcgatcga
     1141 agaaagcggg cgtccaaatt cttgttcaaa taattgatac actgactgga tttctttttc
     1201 ttgtgttttt tcttcgtatt ttttttcttg tgtttttaaa tactcaccta atagatcata
     1261 gatggggtaa agattatatc gatctgcttt tttaccgttg acaatcgtcg tttctatctt
     1321 gataaatcca tttgttacga gacgatccag tatttgaaaa atctgatctt gtttgattcc
     1381 catatccata gcaatcatct gcaaatcagg gaaagtatct ccttcaagct gtgccatatg
     1441 caactgtaga acaaataaaa attcttcaga ttgtagaccg atcctatgat aatttttaag
     1501 taaaagatta gatatgactg tttgtccagc gcttaagtaa ttttctaaat tcaacatgat
     1561 ttctcctccg ctcaatcaca gtatacgtaa aaaaaacatg ggaagcaaga acaggtttct
     1621 tttctattta cttcactgat cggtcagatt tacaatgtcg tctacttgat ttttgtttgt
     1681 tcatgttttt gatgcgtatc ttcacttttc ggaataaata aattaagtag atttcgctgt
     1741 tccttagaat gttgacaagg aaacaaaatt actatacact atttttgttt ccaagttaac
     1801 attattgcat aaaggagttt ttacatgtac agtttcacaa ataaacattc taaagttttt
     1861 ggatacgtct ctgtcttatt cactggatta ggatttacta tcgtcagtcc tgtacttccg
     1921 tttttaacgc tgccttattc tcactccata catcagcaag ctttctatat cactttgctc
     1981 atgtctgttt atgctctagc tgccttctta tctgccccca ttctcggtag cttaagtgat
     2041 cattttgggc gtcatccgat ccttattatc agtttattag gatcaagtat cggatatttg
     2101 ctttttggtt taggaaattc gatatggatg ttgttccttg gtcgtatcat tgaaggattg
     2161 acggcaggag aaatctccac cttatatgct tactttgccg acatcacgga acctaatgaa
     2221 agaaccaaag tttttggatg gatgggtgct ttagtcggta taggaacaac gctgggtcct
     2281 attatagggg gtctattagc tgagttgggg aattctgttc ctatatttat tggagcactc
     2341 tttactttct taaatgccgt ttatggctac acatttatgc tagaaagcct tccaataaaa
     2401 aaacgttcag tagatttaag tttctcccat gtaagacctt ttcaccaatt acaacaatta
     2461 ttcaagattt catccgttat tcctttattg acagcaggtt ttgctgtttg gttggcagct
     2521 ggctctcttc aatctatctt ttctcaattt tcgattgacg cctttcagtg gaaggctggg
     2581 ttggttggtt taagcttctc tttgattgga atcttggata ctgtctcgca attgttcatt
     2641 atgcctcgat tgctcaaaaa attttcagaa cagcagatca caagaatagg gatgttcagt
     2701 gaaatcctcg cctatctatt catcacatta tctggaattc ttctactgcc gattttgttt
     2761 ttatttggga ttatctgtta tggtttcggt gattctattt ttactcctgt attcaatggc
     2821 caactatcca attctgtctc agataaccag caaggtcttg taatgggcgg tacccaaagt
     2881 atacaatctc tatcgcgcgt catcggcccg ctaatcgcag gacagcttta tgctgcagca
     2941 ccatttcttc cgacggtatt cggatttttg cttatgattt ttgcctatta tcactacaat
     3001 aaaaaatagt agcttcattt gctacttcgt ataaattctc tgtatcgacc gataaaacga
     3061 aatactgtgt caaattcttc ttttgacata ttttcaaata cagtagcatc tcgctgtacc
     3121 catttttgat ggagttgctc gtgtctattc cttatttttt gtccgattgg cgtcaattga
     3181 aaatagattt cttttttatt ctcttcgctt tgataagtgt ttatgatccc tctagctatc
     3241 agtttttttg ttagcttact gatggctcct ctagtcatgt aactagcatt cgctaatttt
     3301 gtcacgttag gatcagggat tttcccgata tgttcaatca cttctagttc atttagagta
     3361 aatcctgcaa attctttttc catttctggt ttattacgat gaataatctc attagctaaa
     3421 tccagatatt cttctattag cagttgtctt ttatccattt ctacaccttc tttttctttc
     3481 attatattgt ttttttgttt cctattcaac aaagataaaa atattttgta ggaaaaccca
     3541 atctgaaacc aa
//
LOCUS       DAGCYL010000203         3545 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.207, whole genome shotgun sequence.
ACCESSION   DAGCYL010000203 DAGCYL010000000
VERSION     DAGCYL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.207"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..182)
                     /locus_tag="MB216_002550"
     CDS             complement(<1..182)
                     /locus_tag="MB216_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4527330.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="MB216_002551"
     CDS             517..951
                     /locus_tag="MB216_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBT4527331.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="MB216_002552"
     CDS             974..1294
                     /locus_tag="MB216_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527332.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="MB216_002553"
     CDS             1435..1626
                     /locus_tag="MB216_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527333.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="MB216_002554"
     CDS             complement(1697..1954)
                     /locus_tag="MB216_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBT4527334.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="MB216_002555"
     CDS             2112..2375
                     /locus_tag="MB216_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527335.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="MB216_002556"
     CDS             2399..2587
                     /locus_tag="MB216_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527336.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="MB216_002557"
     CDS             complement(2670..2855)
                     /locus_tag="MB216_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527337.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="MB216_002558"
     CDS             3070..3441
                     /locus_tag="MB216_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBT4527338.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT         1331 a          507 c          704 g         1003 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaag
//
LOCUS       DAGCYL010000204         3461 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.208, whole genome shotgun sequence.
ACCESSION   DAGCYL010000204 DAGCYL010000000
VERSION     DAGCYL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.208"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            21..1211
                     /locus_tag="MB216_002559"
     CDS             21..1211
                     /locus_tag="MB216_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HBT4527339.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            1345..>3461
                     /locus_tag="MB216_002560"
     CDS             1345..>3461
                     /locus_tag="MB216_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4527340.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGK"
BASE COUNT         1213 a          560 c          640 g         1048 t
ORIGIN      
        1 acagaagaga ggacttccct atgaacgatt ttactacaga aattctaaag actctagcga
       61 acaaaggcga tttgaatgaa ttattccgtg tccatttgga gaaagcagtc aatacgcttc
      121 tcaaaacgga gttaacggct ttcctagatt acgaaaagta tgatcgcatt ggttttaaca
      181 cgggtaattc tcgtaacggc tcctatgacc gtacggtcaa gaccgagtat ggggaacttc
      241 atctccagat tccgcgcgac cgcaatggtg agttcaagca acagactgtt cctgcttata
      301 gacggacgaa tgacacgtta gaggagaccg tcattcacct cttccgaaaa ggtattacca
      361 tgtcggaaat cgcagacttg attgagaaaa tgtatgggca ttactacacg ccccaaacca
      421 tgtccaatat aacaaaatca tttacagaag aggtaacggc gtttaatggg cgggagcttc
      481 atgaccgtta tgctgctatt tatatggacg caacgtatat tccgttaaag cggaaaaccg
      541 tcgccaagga agctattcat atcgcagttg gcattcgccc ggacggatca aaggaagtat
      601 tgagctatgc gattgcaccg actgaatcca tcacgatttg ggaggaaatt ttattggacc
      661 ttcaagagcg cggtttgaaa aatgtcctcc tgttcatcac ggatggctta aaggggatgg
      721 taggagcgat tagtcggttc tatcccaaag ctcgttttca acattgttgt gtacacgttt
      781 cccgtaatat cagtcataaa gtgcgtgtcg atgatcgtaa ggaagtctgt gatgatttta
      841 aaatggtgta tcaagcctca tctaaagagg tggcgttgga agcacgtggt acttttgcgg
      901 aaaaatggaa aaccagctat ccaaaagtgg ttgaatcgat tctttcgaac gatcacttgc
      961 tcactttcta tgatttcccc ttggccatac gcaagagtat ttattctacg aacttgattg
     1021 aatcctttaa taaacaaatc aagaaataca gccaccgcaa ggaacagttc caaaatgaag
     1081 agtcgatgga acgtttctta gtctcgtctt ttgatactta caaccaaaaa ttcctaggtc
     1141 gcagtcataa aggtttccaa caggccgaag gcgagctcga acaaatgcta agccaactga
     1201 ttgagaatta gaaacaatct gcagtaggga agaatcattt acacaaaatt attgacgctc
     1261 ccttataatg agaactttta ctaacataat ttatgataaa cttactatat aatcatatta
     1321 attaaatagt taggagatct gcttatgttt atatctactc ttcaaattcg aaattttaga
     1381 aattatgaat ccgaaatctt tcgattcgat aatgagacaa atactattat aggtgagaat
     1441 gattcaggga aaaccaatgc tcttactgca ttgagaatct tattagatga tagttattac
     1501 tatagtagta aaacactaaa agaatcagat ttttttcatg gaattcaaaa tgggtggcaa
     1561 gggcattgga ttattatctc tgcgacattt gaaggaatca gcgaagaaga atttgacaat
     1621 gagatttgtg cttccatttc tttaaattct gaaagtcaaa caatcttaga agaattgatt
     1681 tcaaatgcag ataaaggtgt aggtagtatt tctctattta ttagacctaa caaagctatt
     1741 agaaagcaat tatttgatat ttccactgaa ttagactctc atcaatttaa cgaatttaga
     1801 aattctattc gtctatccga ctatgaattt tactacacat ctaaatcaaa tctaaatttc
     1861 tgtatcaatg aaaattatga taaattggta ggaagactca atgacaatat agctagtaat
     1921 cctgaagaag acgatgaagc tctgttgggc tcaagaattg acatgtcaga tattttcaag
     1981 catatttcgg ttgtttatgt tgacgctctt cgagatgttc ttagagaaat gaaaaacaat
     2041 cgaaacccag tcaaaagaat catggaaaca attgaatcaa aaatatcatc agataatgtt
     2101 gagtcattaa aaacaattat acagcaatta aatgagacta ttaccagtgt tcctgaaatt
     2161 aggaaaattg gcgaaaatat taatcggcaa ctaaatagca taattggttc agtgtattct
     2221 cctaatcttc ttctttcctc aactatgtca gatgatatgg gttctttagc aaaatttctt
     2281 tcaatgaagc cagaacaaaa tatcgactta gacttactag ggttaggaca tctaaatatg
     2341 atttatttag ctttgaaaat agtcgaattt gaagcgtgtc gttcaagaga actacttaat
     2401 attatgttga ttgaagaacc agaagctcat attcatcatc atattcaaaa aactctgttc
     2461 gaaggattaa atcttcaaaa aaactatact cagattctca tgaccacaca ttctgtacat
     2521 ttagctgaag tgtctgaaat atctcggatg aatgtattaa aatcgtttaa tggcaaatct
     2581 atcgttatga atccagcaaa taatctgaac tcatttgcta aagaaaagtt aaaaaagaac
     2641 aatctaaatc taataaacgc agtagagaga tatttagatg ttaaaagaaa tggattgctt
     2701 ttttctaaag gtgttatctt agttgaagga gatgctgaag aaatacttat tcctcaaata
     2761 gtaaaaaaat tatttggtat aaacttagac gaacttggaa taggtttgat taatgttggt
     2821 agtacttcgt ttgaatatat cgctagcttg tttgacgatg atagaataca gagaaagtgt
     2881 gctattgtaa ctgatttaga tgaacaggca atcccatcag aacatcgatt gtataaaagc
     2941 actgcacaag agaaaggtca agcaagaaaa gaaaaattat cctccctttt taatgaaaac
     3001 aattgggtta attgttttta tgctaataca acatttgaaa ttgagttttt agatgtgaat
     3061 aaagaaaata ttctaactta tgtcagtcca attatagatg acgcattcaa agataaaaaa
     3121 acaataactg cttataaaga agaaatacaa agtgaacaat atttagaaag aaatttagcg
     3181 atgttaaaac tagccaatca tgttggtaag gggtggtttg caattgagtt aggtgaaaaa
     3241 attgactttt taatcaaaat tcctaagtat attcttaatg ctattgcttt tgcatgtcag
     3301 gaaatagtag atctctcaat ttataaaaaa attctactcc atacaatcga cttgtacgag
     3361 gaaactacag aattgtcagc tttatcagct agtttaaaaa caagtgatac agctgatttg
     3421 aatatagcaa taatttcttt attagagtac tttggtaaag a
//
LOCUS       DAGCYL010000205         3425 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.209, whole genome shotgun sequence.
ACCESSION   DAGCYL010000205 DAGCYL010000000
VERSION     DAGCYL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.209"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            276..872
                     /locus_tag="MB216_002561"
     CDS             276..872
                     /locus_tag="MB216_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBT4527341.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(930..1412)
                     /locus_tag="MB216_002562"
     CDS             complement(930..1412)
                     /locus_tag="MB216_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527342.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1518..1715)
                     /locus_tag="MB216_002563"
     CDS             complement(1518..1715)
                     /locus_tag="MB216_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527343.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2010..2768
                     /locus_tag="MB216_002564"
     CDS             2010..2768
                     /locus_tag="MB216_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBT4527344.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2828..3256
                     /locus_tag="MB216_002565"
     CDS             2828..3256
                     /locus_tag="MB216_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527345.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3302..3374
                     /locus_tag="MB216_002566"
     tRNA            3302..3374
                     /locus_tag="MB216_002566"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3335..3337,aa:Gln,seq:ctg)
BASE COUNT         1175 a          656 c          561 g         1033 t
ORIGIN      
        1 aatttttaat ggcaatatca taaaaaaaag aagatagaga tttaataatt atatatcagc
       61 tgtttttttc aaataagcaa aaattcctaa aacacaacaa tgaaagcgat aactaagaaa
      121 atccttacat aacgattttt cttattaaaa ttgttttttg aacttcgctt attttttgtg
      181 agcatatctg tttacttact ttttgtaagg tgctatactt catttcgtaa tcaattaaaa
      241 aaatgcaaaa cgcaaatcaa aggagaaatt ttattatgac aacttttact gacactttga
      301 aaaaccgccg ttcaatctat catctaggac gtaatgtttc actttctaat gaagaattaa
      361 caactttgat caaagaagca attaaagaaa gcccaactgc tttcaatgct caatcaacac
      421 gcgcagttat ccttttcgga gatgcacatg aaaaactttg ggaaatcact gaagaagcac
      481 ttcgcccatt gactccagca gaagctttcc caaatacaca aaacaaatta gctggtttca
      541 aaaatggtta tggtacagta ttattcttca aagatactga cgtggtaaaa ggtctacaag
      601 aacaatttga attatatgca gacaacttcc cagattggtc agaacaatca aacggaatcg
      661 caacagctaa tacttgggta gcattagttg ataaaggatt gggcgcaaac ttgcaacatt
      721 acaacccagt tattgacgaa gcagttgcaa aagaatggaa tatcccttca aactggaaac
      781 tacgttcgca attagtattc ggttcaccag aaacaccggc tggggaaaaa gaatacatga
      841 acgatgccga tcgcttccgc gtatttggat aattttattt gaaataaaaa acagaggttg
      901 tgacaaacat cttgtcgcaa cctctgtttt tattctggat caatcataat atcaatctct
      961 gcctcatcgt ttaagcgttg ggacaaaaat ggaactaatt cttctaatga tgcagaaaat
     1021 ttcagcatcg gtcctcgcca tccgtttcgg aaaaaattca tttttacgat tttagaacct
     1081 tttggctgat ataatacaat tttattgatc tcagaaaaag gaacaccttt tttgtcaaag
     1141 ctattcaaaa caagtccttc ttctgtgatt cctctactgt ccaacaaaaa actaataaaa
     1201 atcattgctg acaagatccc ttttgccaat tcatctgctg taggatgact gatgagtgta
     1261 taccaaatga ctagtgacac taagaagggt atcaccaagt agacccatcg atttcgcaca
     1321 gtccgataaa taatttcttt tcgaaccagc caaagaaata aaaacatcac aacaacgaat
     1381 aaaaacaata gaaccgtaaa gaatgttacc atgacaactc gcctcgcttt ttcttctcca
     1441 gaaaatagga aaagaatact gatttacctt ctcaaagaaa ttggacttgc agcacgccgc
     1501 acgtccaatt gttttcttta ttcaaatgta tcatattttg caacaaccat gcttgctttt
     1561 tcatctaata attcttggtc ctctgcagca cattgggcaa ctttgaccac ggcgcagttt
     1621 gtcatttttg atacaacttc accaatgacc gtttttgtaa aaccaattgg tttgcatgca
     1681 tattgttctc ctaaattaat catttcttcc gtcatcaaaa tcctcctaaa ctgctggaac
     1741 ttttctccac attaccactg ctatttcttt ggagatttaa ctcattgatt tttatttaca
     1801 gttaaaaatc aaccttacag gcattatagc gtactgtttt cgttttcaca attaaaacag
     1861 ctgttgtatt tagaagaatt tttttcaaaa aattaaaaca tattccttaa aaactaatta
     1921 tttcctacat attaaaatat aatacttcca tttccaataa aaatacattt ataataggaa
     1981 agataaacag ctgaaaaggg agatttcaaa tgaagaaaaa aacgttcaaa aaactattgt
     2041 ttctaggggg cacagctgct cttttattaa gcggtacaag ttattaccta catatccatt
     2101 ctccagaaca tacaatgaac actgcacaaa aaagttcaac acaaaaaacg aaaaagacaa
     2161 aagagaaagc agaagtttta ctagatgttc cattagagag ccagtttgat tctccttctt
     2221 tagaaaatgg ctgtgaagtc acttcgcttt ctatgctttt atctttttat ggttatgaaa
     2281 caactaaaaa ccagttagct gagcaattgg attacgtccc tgttttcaat gcagatggga
     2341 ctcatggcga tcctaatgaa ggatttgttg gtgatatcag tggcggagac tgggctatgg
     2401 tcgtctatgt tcccccagtt gcctcccttg ctcagcaaat cgtccaaacg gattatcaca
     2461 cttttccaaa aaccgatgct caactcgatg atatcaaaaa agcactatct aaaggaaatc
     2521 ctgtctggac ttctgtgact atcaattttg aagtcccaga tgaaactgat tttatgactt
     2581 ggaccacaaa taatggagaa gtaagagtca ctcctcttgt tcatgcttgt gtagtcactg
     2641 gatatgacaa agaaaatatt tatgtcaacg atccctatgg tgtaaaaaat cgagcagtac
     2701 cgatcaacga tttttcggcg attttcactg caatgggtgg acagatgcta actttggaga
     2761 aaagctagta attcaaacaa aaatccctta tactcaagat accaaacctt tgaagggagc
     2821 gtagataatg gaaaaattca cacataaaaa aatggatcca aatgaaattc cgattatttt
     2881 tgtccgtgac tgcaagggaa atgttcaagg taaagtctct attaatgagt ggaacgagcg
     2941 tcgcagacct gctaccctca atgaactgga aatcaaattg tatcgtcaat ctcttgttta
     3001 ttacgcagat caagagtacg agaaagccac cgatttattg aaatttctaa ttgctcgaac
     3061 agagtatact cgctttgaat atattgaacg tttagcaaac atctatcata ttatgaatga
     3121 acctgtcaaa gaatatcagt tattggacac cgtcttgtca gtagcagaac tgattgccct
     3181 tcctgctgga ttagaaaaga aactagtacg ccgcctttta cgagtaaagc aacaactatc
     3241 cgatcaagaa aaataacttg cgttttttta ggaaagtgct atgattattt tgttgaataa
     3301 atgggatgta gccaaactgg taaggcactt gactctgaat caagcaattg taggttcgaa
     3361 ccctgccatc ccaatttgat gtgcttgctg aaaaaatgaa ccccctgtaa aagaacaatc
     3421 ttttt
//
LOCUS       DAGCYL010000206         3406 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.210, whole genome shotgun sequence.
ACCESSION   DAGCYL010000206 DAGCYL010000000
VERSION     DAGCYL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.210"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            108..692
                     /locus_tag="MB216_002567"
     CDS             108..692
                     /locus_tag="MB216_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527346.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            703..948
                     /locus_tag="MB216_002568"
     CDS             703..948
                     /locus_tag="MB216_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527347.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            954..1676
                     /locus_tag="MB216_002569"
     CDS             954..1676
                     /locus_tag="MB216_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527348.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            1807..2550
                     /locus_tag="MB216_002570"
     CDS             1807..2550
                     /locus_tag="MB216_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527349.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            2556..2921
                     /locus_tag="MB216_002571"
     CDS             2556..2921
                     /locus_tag="MB216_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527350.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            2937..>3406
                     /locus_tag="MB216_002572"
     CDS             2937..>3406
                     /locus_tag="MB216_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527351.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFM"
BASE COUNT         1349 a          443 c          624 g          990 t
ORIGIN      
        1 tttatttgta tgacaagttt accataaaaa aaaattaaaa agcgagacat atttatgttg
       61 ttgtgttatc ataataacat aaatatgtag agtgagatga tgaaaaaatg gatttgaaac
      121 aatcgtttta catctggtta tatcctgata atcctatgtc aataatggct gatatttttg
      181 tagttagcat cttattgtat ttattttatc taattttctt tcacaattta atcgggtaca
      241 tgattcttga ttggaaagga atgttcgata tagaaagaaa accatttgct aacaatcaaa
      301 cctataccgt cttcgaaagt ggagattggg aagaaaatga agttgatgtg atccatgaaa
      361 ttggattagc tttatcagga atacaagatg ctgatgtttt ggtatcaatg acaacggata
      421 ctggatacaa cgcttatgag gtattttatc ttatgtattt tatatggcgt gatggtcaag
      481 aaattatcaa tggtcgtgct gaaaaaacaa tcaattctaa tactgaaatt tctttttgta
      541 tgggtgaagg ggaaatgatt gtatataaag atgggtcagc aaacattaaa acagcctttg
      601 gtgatattga aacaagtatt aaggaactta gggaatatgt gcagaaccca tataattttc
      661 aatatttagc aggaagaata aatttagttt aggagtaaaa atatgaaaaa aattataaat
      721 caaccaacaa atcacttgat aattgaaggt gttattttcg taacaatgta ttttactgta
      781 ggaaatggca tctcaaatac aatcattcaa aaaaatccac aatacctatt atttagtctt
      841 tatgcttgga ttgtacaaat ggtttgggtt tccatattga tgctgttgtt ttattggata
      901 gtacgggtta ttcttttatt gttaaacaaa agaaaaaagg ataggtgaat aaaatgaaat
      961 caactaaatt gaaggttgta ttgttatcga cagcagcatt aataaccctt acagcatgtg
     1021 gtacaactaa tagtgctaat aataacaaag acacaaagtc tagtagtacg atggttacta
     1081 aaaacagcac tacatcggca tcaacaacaa tgtcttcaat gactgaaaat aatgctgaaa
     1141 agtatcaaaa atacgaagac attaataaag gtcaaaagcc ggatgaagtt tataacaact
     1201 ttattaatac agtaaaaaat gatgaaaatt atgggaatga tccattgcaa aataaaaatc
     1261 tgactatcgt tctttacaaa cctgattgtc cagattgtca aaaagcagaa tcaaaaatca
     1321 tgcaagaaat aaaagaagga tttagttatg ctccaacatt aatgcatgat attaccataa
     1381 ataaaccagc ttacgagtat tacgcagttg ctgtagatat tactgatggt gtacctgagt
     1441 gggtacaaaa cattcctggt ctggctgatg agaacaatca atatcatacg ccaacagttg
     1501 cagtagtaac accaaagaaa gttaacggca cactatactg ggatgtaatg ggaatgacaa
     1561 caaatacaac tgatgaaatg attgaaagta caactaaata ttatatggat ttgggagcat
     1621 ataaattata tcaacaatca gagcctgtta tggacgatca atccaaagtt aaatagatct
     1681 ttagggcgta agccctattt ttttaaaatt aagtgataac aaataaatga tttctgaaac
     1741 aaacgttctt actcaactac tcgaaacaca caagctaatt aactagatta taaaggagaa
     1801 tgcgtaatga aagaaatcag tgcaaatgaa gctcatattc cgcaaaagaa tcaggttggt
     1861 atatataagg tgcttcatgg tgaaggaatt tataaaatac agttacttac cggttacaat
     1921 aatggtgcaa atgggaagtg gttaaaggca acaagtattc gtgaacttag aagtgaagta
     1981 gaaaaaatta ggcttggtct cacaagtgaa gaaatagcta aactgaaggg aaataattct
     2041 tttgtaattg aaaaagaaaa ggaaaaacat ctactttatc gtgagctgaa aattgaagaa
     2101 tactatgaaa ttgatttacc tgaaaaatta agaattattg atcaacgttc agaagaaaaa
     2161 tggaaaactg aaatatatcc aagcttaaat gactacaaga catcaggaaa gcccatgtat
     2221 aactacacag gctttttaac ctacacacaa tacaaatatg cctttcgcaa attaaaaggc
     2281 attaaacaag ctgaaattgc tctgtatgag atgaaaaaag tggatagatt gaaattgatt
     2341 caagatttag tgcaaaatac atggaatcaa gcgattgtcg agctagaaaa agaaaatggg
     2401 aaaattttaa ctaataaata tctgatcgta aatgaaattg aagaattaat tggtttattt
     2461 tcatgtgaaa aaggtgaagt cttcttgaag tcatcaccaa cctattcaat tttggacagt
     2521 ccgtatcagt ttatacaggc aaggagatga gttagatgtc tagcttgaaa gatgactata
     2581 tccaagcaaa acaagagtat gaaaaaatac agatgttgaa atctgagaaa gtcgaattat
     2641 ttttaacaca aaaattagca gaggatcata ttgctaactt agatgctaaa actttagagg
     2701 aatatgatcg gcagtttaat cagatacatc acaatatcat tttgcttgtt gagcaggcaa
     2761 agcgaaggtt tgaaaaagcg gaatatgatc tattagatca aggaattgta tgcttggata
     2821 agggattgcg aagatattat ggtgcagagt taaaaaagca aaatgtagat agaaatgcac
     2881 taaaacatga aatcttagaa ttgataatgc aacgaatttg aagggataga aagcaaatgt
     2941 cacaaaacat tagtgctgta aaacaattaa tagaagaaga tttagagacc acagttcaga
     3001 gattagcgga acgaatccat tttgcaaatt atcaaaccat ctctaactgg tcaagcagga
     3061 acagaaatcc ggaagaaatt ccaatgaaat ttttttatcg aatcggaaaa gaatatggtg
     3121 ttgatccgat aaaaattttc gagaagacca tgatttatta ctatcatgga acacctaacg
     3181 aagtgttaga acatcttttg gaagaggcaa aagaaaaatc atcaatatta gagctatcgc
     3241 aaaatgacaa agttattcaa gtgaaatgcg ttgatccaac aggaaagatt attgtcggtt
     3301 ttgatattga aagaaaatcc aaaatagatt ttattacaca attaaaaaag ataaataatt
     3361 tagatattct aacgcttaga ccagatcatg aacagttgtt tatgag
//
LOCUS       DAGCYL010000207         3322 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.213, whole genome shotgun sequence.
ACCESSION   DAGCYL010000207 DAGCYL010000000
VERSION     DAGCYL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.213"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..2232)
                     /locus_tag="MB216_002573"
     CDS             complement(<1..2232)
                     /locus_tag="MB216_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527352.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="MB216_002574"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="MB216_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          911 a          744 c          536 g         1131 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aa
//
LOCUS       DAGCYL010000208         3316 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.214, whole genome shotgun sequence.
ACCESSION   DAGCYL010000208 DAGCYL010000000
VERSION     DAGCYL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.214"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(457..819)
                     /locus_tag="MB216_002575"
     CDS             complement(457..819)
                     /locus_tag="MB216_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBT4527353.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            1042..1794
                     /locus_tag="MB216_002576"
     CDS             1042..1794
                     /locus_tag="MB216_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4527354.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1934..2857)
                     /locus_tag="MB216_002577"
     CDS             complement(1934..2857)
                     /locus_tag="MB216_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4527355.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2872..3144)
                     /locus_tag="MB216_002578"
     CDS             complement(2872..3144)
                     /locus_tag="MB216_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527356.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         1087 a          622 c          538 g         1069 t
ORIGIN      
        1 gaatacttat tttattcaat gtatccgaga cacgaagaaa ttagcgtttt ctatttttct
       61 tacttgataa aataagtaga ttttcctact acaatcgtag ccgttaagat accgatgatt
      121 gaacaaatat aaccaaatgt ctctacaaca taatcaatgg caaaagtttt tgaagcaata
      181 atacgtcttt ccttttttgc ttcttatccc caaagaaacg gatataactt attatgtatt
      241 gaattttttt ggaaaggcga attatgacaa gatatacctt gcgttgaaaa aactcgcgga
      301 aaaaatatca cacgttttta aaaagataca aagtaagaaa gcagctgtca atagaatgga
      361 aacaaaaatg gcgagacatc aatcagtctc gccttgtttt ttattgtata cattagatat
      421 ggagcaggtt accataaaga tgattattag tcgttattaa atagaatcct tcgtattttt
      481 ttgttctatc actacaatat ctttatttgg acgtttgaat tgtattccgg cacgttctag
      541 ctcctgtttc actcctacat ggactttgac atcttctcgt gtcattttta aaacgtgatg
      601 aatcgcatag tcgatttctt gccttgtgat ttttttcttc gtattgatca tcacgacttg
      661 ctttttatca tatgtgtcta ccaatagcag ttgtgttcga tctaacgaaa ataatttgta
      721 ataattaata gccgtattcc caaaaaagaa ttccactact ttaggataaa atgtcttaat
      781 atttaaatta ctattcacag gtgtttcgat tagcttcatc tcattcctct tttctatata
      841 gtcgatttag atccaatctt aagttatgtt accatctttc ctagcattaa ggaatcggtt
      901 acaattgaac ctttaaagac aaaaggattt accttttatc acttaatagt ataacataat
      961 ttcgtgagaa aaaaatacaa ttttttcttg accatctcgt tacgtaaagg attatgctta
     1021 catttgtcag gaggaaaaat aatggagtac acaataaaga aaatgtcaga gatatcagga
     1081 gtcagtccga gaaccttgcg cttttatgat gagattggac tgctaaaacc agctaggatc
     1141 aattcttcag gttatcggat ttatggcaaa aaagaagtcg atcgtctgca acacatctta
     1201 ttttatcgaa caatggcatt caagttggat gatatccaag aggtcttaga caatccatct
     1261 tttgaccacc agaaagcttt aatcaagcat cagcaaatgt tgctggaaaa acgggcacaa
     1321 atcgataccc tcttgacaac tgtccaacag acacttgata tgtacgaagg aggaagaaaa
     1381 atgagtgaca aagaaaaatt tgaagggttt aaacaacaaa agttgaaaga aaatgaagaa
     1441 tcctatggac aagagattcg acaaaaatac ggaaaaaaaa aggttacagc atccaatgaa
     1501 aaatggatga atatggataa agaaacgtat gaagaaatgc aaactgttga aaagcagatg
     1561 ttatttgatc tcactcttta cttaaaagaa ccagaagatg aaagcttagc agaccgtatc
     1621 ttccaagctc ataagaagtg gttctcttac agctggcctg aatatcagcc aaaagcgcat
     1681 aaaggactag gaatgatgta cgaagccgat gaacggttca ctgcatatta tgacgagcga
     1741 agtggtcatg gtgcagcaaa agctttaaat gaaattatcc aaaaaaaggc gtaatctttt
     1801 acatgatcca acaaaaaagt acacgtaaaa tgacctaata acaaaacagc agacacccaa
     1861 gttagatttt tatgctaact tttggtgtct gctccattct ttcacaagtt ttttcatcct
     1921 actttaaata tattcatcgt tgaataaacg tataaggtat tttcacttta tccttttttt
     1981 ctgtaagaaa caattgaaat gccatttctc cgcaagcttc cagatggtgg tcgatggttg
     2041 ggaaaccaag aagctcgctg atcagcaaat tatcgcgtcc aatcaccact ggtgtcttat
     2101 cttgcagata gctttgcaag atgcctgctg caaccatatc cgaatttgta aagacaccat
     2161 caactccaag ctctttaaaa aagaagcctg cttgttttcc tttctctgca tctatacaat
     2221 cccaaaaaac aagtgattca tcaaaagatg gaaaaagttg atgacatagc tgcaaagtga
     2281 tttttgagtt ataacttagc ctgccacttc gccccaacgt gatgcccaat tgctttatac
     2341 cttgctgctt caaataagta agaccttctg taattgattg ttctcgatca ataaagacac
     2401 agcttgcctg ttttcccttg atttcttcac aaaaaactag aggtccatat ttttgatatg
     2461 ccaataaatc atctagtgta ttggcacgag acgtaatgat caatccatcg aaagctttag
     2521 ctgcaaattc ttcaagatac cttcgctctt tttctttatg atagcccgtc ggtaaaagtg
     2581 tcactttgta atcataatca aacgctgctt caatgatccc actgatcaac tgatcaaaat
     2641 acggatgatt ggtataaggc agaatcactc caatgctttt cgtttttcca taacttaact
     2701 gtctggcatt tgcattaggg atataatcga cttctttcat gaccgcaagt atttctttcc
     2761 ttttttcctc atctacatat ggatgtccat tgattacacg tgaaatagta gaaactgaat
     2821 aacctgtgag ctttgcgata tctcgaatgt ttgccactgc tgcttcgcca cctatacaaa
     2881 aagattttct tgtaaatagt ctaccacagc tggaccgcat tcaaaaaact gatacatttt
     2941 tccttcatgc gaaaaactga tccgctgccc gccccaatat atatcttttg tcaaattttc
     3001 gatttcattc attggtattt cttttactat attcctattc aacccttgaa aactgtacaa
     3061 cgttaatacg gatcccttga actcgacaaa cgcataaaaa taattgttcg ttgtgccatc
     3121 tgttatctct acataagtat tcattgcaag ccctcctttt gatactttga gtatagtgta
     3181 tgaaacgcgt ctcatagcaa ggagtttgtt ttgccaaaga aaagttttat tcctctttca
     3241 ttattaccaa agtctcttat tttaaattga atacattata atgatactgt aaatatatct
     3301 ttattattag tgaatg
//
LOCUS       DAGCYL010000209         3272 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.215, whole genome shotgun sequence.
ACCESSION   DAGCYL010000209 DAGCYL010000000
VERSION     DAGCYL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.215"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     regulatory      153..197
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            221..1195
                     /locus_tag="MB216_002579"
     CDS             221..1195
                     /locus_tag="MB216_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBT4527357.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            1200..1775
                     /locus_tag="MB216_002580"
     CDS             1200..1775
                     /locus_tag="MB216_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4527358.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(1842..2981)
                     /locus_tag="MB216_002581"
     CDS             complement(1842..2981)
                     /locus_tag="MB216_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBT4527359.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          918 a          641 c          641 g         1072 t
ORIGIN      
        1 gattcttttt cttcggcaca cgtaaattcc atttcttttt cattgtatca tttacttaaa
       61 gttcttctca atagatttac ttgtaagaga gcatttacat tcacaataaa agccagtata
      121 ctaatttcgt gcattataaa caagacactc gtcttggact ggttcggaaa cttcccagaa
      181 taaaaaacta agtatctcta aagaaaggag attggaaata atgcgtaaag taattattga
      241 ttgcgatcca gggatcgacg acactttagc tttatcacta gctgtcaaat cccctgatat
      301 cgaagtagtt gctattaccg tcgtttgcgg gaatgtccct gtagatatcg gtacgcagaa
      361 tgtgctgaaa tgtttggagc gctgtgatcg tttagatatc cctgtttatc aaggaatgga
      421 aaaacctttg aagcaacctt tcattagtgc gcaagacact catgggttgg atggattagg
      481 ggacacaaat ttcccgatgg tcttagccaa acaggcagaa tcgctccatg cagttgattt
      541 tctgactgat tatttcaaag aaaaaacaga tacatcagtc attgctttgg ggcctttgac
      601 caatatcgct tctgctttga aaaacagccc atctgttggg aaacatatgg atcgttttgt
      661 ctcaatgggc ggaacttaca aaagccatgg taactgttca ccagttgctg aatacaatta
      721 ctggtgtgat cctgaagcag cttcttatgt attcgaaaag ctcaaacaac cgatagaaat
      781 ggttgggtta gatgtgacaa gagaaatcgt cttgacaccg actatccttg aatattgctg
      841 ccaaatgaat cctgaagagg gagagtatct aaaagcgatc actcgttttt attttgactt
      901 tcactggaaa caagaaagaa tcttaggatg tgtcatcaat gatccattag ctgttgctta
      961 ttttatcgat gagtccatct gtgaaggctt taaaagcttc actgctgtag aaactcaagg
     1021 aataagtcgc ggacaaacat tggttgatcg ttatgaattt tggcagaaac ctgctaacag
     1081 taaaattatg acaaaagtag atactcgtct ttttttccga aagtttttaa ccgttctttt
     1141 aaatgcacaa gaagaaacga ttatgaaaga tttggaaagg ttaaagatgg gctaacaaaa
     1201 tgaataaaaa caaaatggca actcgcgtac ttacagtgat ggctttgtgt attgggatca
     1261 attatattgg aggaacgatt gctttatggc ttcgtttgcc aatctatctc gattctatcg
     1321 gaacgatttt tgctggtgca ttgcttgggc ctgtacctgg aatgctgaca ggattgtcca
     1381 gcggtatttt aagtggagtg actacagata ttttttctct gtattattcg ccaatccaaa
     1441 ttctcacagg cttgcttgct ggattgattt tgccaagata tctcggcaaa gataaaaaca
     1501 gcttgaaact gttattactc gctttgattt tgtcggtacc tggaacgatc gtttcttcga
     1561 tcattaccgt tcagttattt ggcgggatca cctcctcagg atccagtatg atcgttcagc
     1621 tattacatgg gttaggttta agtcagacaa caagtgtgat cttggtacag gtgggtacag
     1681 actaccttga tcgcttgttg tctatcgtcg ttgtttcatt agtcatcact atgttgcccc
     1741 aacgcttgat atattcaaaa agaaaaatag gctgaataaa cagagctgga ttagcaaaat
     1801 aatgctaatc tggctctgtt ttttcattta ttaaaaaaat cttacgctaa gtaggataaa
     1861 ataattcctt gatatttttc gatcatatcc aaataattat cgatttcaac atactcatct
     1921 acttgatgag gcaaagtaac gacaccaggc ccaaagacga caaagtcgaa ggaatggtta
     1981 gctttcgtaa attcggctgc atccgttgtt gctgccgctc caactaaagg taatggctgg
     2041 ctgaattgtt gctgaatgga atgaatcaaa ggagaatctg gatcagcttt gacaggtatt
     2101 ttgttgtaat cgatcatcaa ttccaaatga tagtctgtct cttgattcaa ttcattgacg
     2161 attgattgca gcaatgcaat gattttatca tttgggtatt ctggaatcga tcgaatgttc
     2221 ccttggagct gcgcatggct tgggatgctg ttgacttgat tcccgccact gatcaatgta
     2281 acattatgga ttgtttttcc taggacagga ttctcgattg tttcagcgag gtgattcatt
     2341 tcagcatttg ctttcgtaat gaattcattt aggtgattga ttgcattata accttggtct
     2401 ggcatagagc tatgcgcttc ttttccgtgc gaggtgactg tatagttgat tgatcccatg
     2461 tgcgtataca tcaaactgta attggttggc tctccgatga tcaaggcatc taagtcatct
     2521 acatagcctg ctttcgtgag ctgttcccca cctagttcac ccacttcttc tccgacagtg
     2581 gctaataatt tgactgttcc gttaaatggt tttcctgact ctttcagttc gatcatggca
     2641 atcaccattg ctgccaagcc actcttcata tctgtagcac cacgtccgta caaacggttc
     2701 ccatggattt ctgctgcaaa gggtgcatag gtccaagacg attcatcacc agccgccacg
     2761 acatccatat gtccagatag gcctaacact tttcctgatt ggcccttttg gtaacgagca
     2821 atcaaattat ctctaccatc tctgtaagaa acaatctctc ctgttatgtc atgccttgcc
     2881 aaaagcttat tcaaataggc tgctacttca ccttcgtttc cattcactga tttaattcgg
     2941 ataatctctt gtaaaatcgc gattttttct tcttttttca tctaatcctc tcccttgcca
     3001 tttcttttta ttgtatttga gtgtgctcga attttcaaat aggagcaacc aaagtaaaaa
     3061 tgaagaaaga gagccacttt atgaaaaata tgctgctact tccctcctat tttatttata
     3121 cgagaggact tgcccataga ttcctattct actgttctat ccttaaaagc tcttatatga
     3181 tgctagttat tgttagcttt cctttttcaa ggatgatctt ttcttgtacc attctttgta
     3241 aacatcatat acagaagcta gttgcaaaaa aa
//
LOCUS       DAGCYL010000210         3188 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.216, whole genome shotgun sequence.
ACCESSION   DAGCYL010000210 DAGCYL010000000
VERSION     DAGCYL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.216"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            115..1854
                     /gene="yfcC"
                     /locus_tag="MB216_002582"
     CDS             115..1854
                     /gene="yfcC"
                     /locus_tag="MB216_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBT4527360.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2011..>3188
                     /gene="iadA"
                     /locus_tag="MB216_002583"
     CDS             2011..>3188
                     /gene="iadA"
                     /locus_tag="MB216_002583"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBT4527361.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     "
BASE COUNT          974 a          537 c          750 g          927 t
ORIGIN      
        1 aagtgatttt ctaataaatt attcatgtgg aaatgtcatt tttactgtat aattcatttg
       61 attatccatt ttttattcat caaaattact gaaaaaagaa aggaagaaat aaatttgaag
      121 aaaacaaatt cttcaggaaa accgaaacgc gacttcacta aattatcgac accccatacg
      181 tatgtcatta tttttggtgt agtcatcttt gcatggatac tgacttttgt cgttccagca
      241 ggaaagttca gcacgcaaga tattgaatac aaagatgcaa atggcgaaac aagtactcgt
      301 actgttttac gtcaagattc cttccgttat gcttacgagc tggataagtc gtatgtgttt
      361 gatcaattag aagaactgca agatcatcca gcagaacgtg aaaaactgga tgttccggaa
      421 aaaggattgg aaaaagtcat tgctgacgga gaaaaaaatc ttacgcagga aaaactagat
      481 gaaatctcct tgacagatga tgtgctttac gatcagtacg gcgaaaatat ctatgatact
      541 tctaaaaaat tgcataaaac agccaaaata tggggaacag atgattttgg cggctttggt
      601 ttcttaaatt ttgtttttga aggactagta tctggagata agtatggatc tgctgtaggg
      661 atcgctgcat tgatccttgt tgttggaggt gcttttggta ttattatgcg taccggagcg
      721 atcgacgcag gtatctatgc gtttatcagt aaaactaagg gactggagcg tttagcttta
      781 ccgctattat tctttgcttt ttcattcggt ggcgctacat tcgggatggc agaagaggta
      841 attccattct caatggtgat ggttccattt gtcattgcgt taggatatga ctcgatcgta
      901 gcagtaaccg tgacttatgt cgcatctcaa gtcggaaatg cgacctcttg gatgagcccg
      961 tttagcgtag cggtggctca agggattgct ggaatccccg tgttgtctgg tgcgacattc
     1021 cgattgatta tgtgggtagt tgttactgca ttagccgctg gttatatgat gatatatgca
     1081 gaaaaaatcc gtaaaaaacc agaaagttcg ttgacttaca aatcagatga ttatttccgt
     1141 tctcatatca aaaagacatc tgatgaaaat aaaaaattca tgcttgggca taaattgatc
     1201 ttattagaga tgttagttgt ccttgtctgg atcgtttggg gcgtgactca aaaaggctat
     1261 tacattccag aaatcgcttc tcaattcttt gtgatgggat tagttgctgg aatcattgct
     1321 gttcttttca aattagatgg aatgaaagtc aatgacatcg cttcttcttt ccaatctggt
     1381 gcagctgatt tagctggtac agcaatcgta gtaggtatgg ccaaagggat tcttcttgtc
     1441 ttgggaggat ctgatgcatc tgttccttca gctttgaata cgattttgca tgggatcggg
     1501 acggcgttga ccggtgttcc agctgtcatt ggtgcttggg cgatgtacat cttccaaagt
     1561 ttgttcaact tggttgttac atcaaattca ggacaagcag ctttgactat gccaatcatg
     1621 gctccactgg cagatttagt aggtgtatcc agacaagtag cagttcttgc ttatcagctt
     1681 ggttcaggtt ttatggacgc ttttacaccc gtatcagcca gcttgattgg tgtgctaggt
     1741 gttgcacgga tcgaatgggc aaaatgggca agattccaaa tcaagatgca aggattcctg
     1801 tttgttttag gaactatttt catcatgatt gccattgcaa taggtctgca gtagtactgg
     1861 agacctgtaa ataaaaaagt aatccaaaca gtagataagg actccaagaa ccgtcatttt
     1921 acggtaaaat caaaggagta aagatcctat cgtttggatt attttttaag atcctatcat
     1981 cagttatact taaagaaaga ggaaaaatgt atgaaaatca ttcgacaaat tgaagtgttt
     2041 gcaccagatt acttaggaaa aatggatgta ctgattgccg gtgggaagat tttggctgta
     2101 gaagaacagc tagaaggcgg ctacgaagga gtggaagtag aagaactttc aggtgaggga
     2161 aaagtactaa ccccgggttt cattgactgt catttccata ttttaggtgg aggaggagaa
     2221 ggcggttatc aaaatcgaac gccagaagtc acattgagcc aattgactac agctggtgta
     2281 acgacagttg tcggttgttt gggaacagac ggagaaggta gagatatgac tgcgttgatc
     2341 agcaaagcga aaggtctgga agcagaagga atctccactt atgtatacga aggttcgtac
     2401 cgattgccgg taaaaacagt aacagattct atcatcaaag actttttgac aattgataaa
     2461 attatcggaa tcggagaaat cgcagtatcc gatcaccgtt cttctcagcc aagttttgaa
     2521 gagttcgctc gtgcagtagc agatgctcgt gtaggaggca tgttatccgg aaaagctggg
     2581 atcatcaatg tccacttagg cggaggaaaa agaaagctag agctattgac acgaatcgta
     2641 gaagaaacag aaattccgat cacacaattt ttgcctactc atgcaaatcg cacaccagaa
     2701 ttatttgaag cttgcgtggc atttgccaaa cgtggtggca cgattgactt cactgctagc
     2761 gaagatcctg atttttggga gaagacagat ggtgaagttc gcttcagcaa agcactgaaa
     2821 cgattgatcg aggaagatgt ttctttagat aatttcacga tgacctcaga tggacaagga
     2881 agcttgcctt attttgatga aaacaatcat ttcctagggt taggagtagg aagtgccaag
     2941 gcattgctag tagggatcaa agaagctgtg caaaaagaaa gtatcccttt ggaaatcgcc
     3001 ttacgagcta taaccagtaa tccagcacgt atattgaaat tggataaaaa aggaaaaatc
     3061 gaaattggtg ctgatgcaga tctgtgtatc ttggataaag aaacgcttga tattgataca
     3121 gttatcgcta aaggagaaat aatggtccaa gaaaaagaag tcaaagtatg ggggactttc
     3181 gaaaaaag
//
LOCUS       DAGCYL010000211         3061 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.218, whole genome shotgun sequence.
ACCESSION   DAGCYL010000211 DAGCYL010000000
VERSION     DAGCYL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.218"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            626..1144
                     /locus_tag="MB216_002584"
     CDS             626..1144
                     /locus_tag="MB216_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBT4527362.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1036 a          449 c          534 g         1042 t
ORIGIN      
        1 tataataatt catgaagaga ggtaattcac ttatttaccc aagctgaaaa gcctgatatt
       61 acttcttttc aggcagtgct gctaatattc aattacactt ttttgtataa atttagttgt
      121 tttaaattta attgtctgct atgcttgtga tgaattgatt ttcgttctgc acttttggac
      181 gataaatttg gacaattgaa aatacaaaaa aaaagaaagg ccctgtgaga ctcggaaggc
      241 taccaacctt cgtcgacgtc cacaggtggc attaagatca ctcccttaat gcgtttacct
      301 ttcagtgtta cggtattacc cgtaacggat ttagtataac agatattcaa aagtgatacc
      361 actttttttg gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag
      421 cataatgtta taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagga
      481 gacgttggga tattttataa atcattcaca tatgagttag acagtcaatg catatcagat
      541 atgcattgta gaacggatta aaatatcgtg gatgaaatta tagatgaatt aaaggagaca
      601 attaggaatt tatggaggta atataatgag aacatataaa aataaagaag aattaaaaaa
      661 agaaataaat aggacttata ctaaatatat tttagaattt gaaactattc ctgaagaatt
      721 gaaggataaa agagttgagg aagtggatag aacacctgct gaaaatttag catatcaagt
      781 tggttggacg aatttaattc taaaatggga aaatgatgaa agaaatggat tatctgttaa
      841 aacgccttct gatcaattta aatggaatca gttaggagag ttatataagt ggtttacaaa
      901 tacctatgca catctaccat tgaaagaatt agaggaaatt ctcaatcgta atattgatga
      961 tattaacatg atgattgatt caatgagtga tgaagatcta ttcactgcac acaaaagaaa
     1021 atgggcagac gaggcaacca aaacggctgt atgggaagta tataaattta ttcatgtaaa
     1081 tacagttgct ccatttggga cttttagaac aaagataaga aaatggaaga gattagcttt
     1141 gtaaattccc gtttactgct aagcaattgt ggaaaggaga cctcttgtat gttaacagta
     1201 agaacatacg actacataag ttctttgcta actgccggtt tttattgggt gaatgcaagt
     1261 gcactcccct agaatgccgt agagggtgca tcgttagatt gtatcaattt tggttggcca
     1321 acagataaac catgactcac ttcgataaaa tgagattatg gtgccatatc cgctacaaag
     1381 ggacttagga gactagcagg ggaaagattt ttttattttt gggatgtttt tcagaccgcc
     1441 gatagtaccg acaattccct ttaaatatgg aatctcaaca acccacatga agggtaaggc
     1501 aagtccggta attatatctt ctgactttgg gttaggaaga attgaaattg ttaagaacga
     1561 ttcaacgctt atggtttata atttggagat agcctttgtt aatatgctaa agccaacata
     1621 cgatacagat tttgagcagt ttattctaac catcaaaggg tatgtggcct atcgcctatc
     1681 ctattactga tactgaagag atcttaaata aattctatac gggatttgaa gatcctgaag
     1741 gtgaaatcgt catttatcag aataaataaa tcaaatagta gaagttcaaa gaatgagggg
     1801 aacattagtt tttattgctt agtttaatcc cagtaccgat ttctgcaaga tgtaaaaggt
     1861 ctttcatttc ataagttttc agaagcaagt tcatacgcac taactcactt tcgattgagt
     1921 agcaatcgtc aacaaacttt actatcagct gttattttta ttctttcgtg ttatctaaat
     1981 accatcaatt tataagttgt aggtgtattt aataaaagaa caaataactt tagtattttt
     2041 attaatctaa cttacaaact agtagtatag gggtaaattt gcgattattc ataaatatac
     2101 caagtaaaag tatagggtat agtaaaaata tgataaaata aatagtcgtt atcttaatcc
     2161 aactatatag aaaatttacg ttttctatat agttggatgt ttacaagtta tatctggttt
     2221 aataagtttt tttctgttag tcatggcatg ttttattgtg ctggttcatc aatatattga
     2281 aatgtactaa aatgaaattt tcagttatgg tataatattt atgctgtctg ctaccccggc
     2341 ctattatggc aaggggagga ggttgtatta tgaattacct tttcgatatg ttttttgcaa
     2401 tcttagcaga agtatccgcg cattacatct gtaaatggct tgattgcaaa attattgcca
     2461 aaaaagacag taagcctacc cgttaatctg aatagaacgt aaaagaaaac cactagcccg
     2521 ccagctagtg gtttttggtg tatcatgaat taccttttcg ttaaaacaat tataccatat
     2581 ttcctaaaaa atacaattaa tagacgaatt atcattacca ctttgatgaa aaaagctgac
     2641 ataaattttt cttttatttt tataatgaat aaacatttat caataagctt ttgaaaggca
     2701 ggatcgtact ttgttgatga ttgagatatt gctacatcaa tctcagcagt taagattttt
     2761 ttgaattgtt gcttgaattt tttctttatc taaaaaaatt gtatctccag gatgcaaccc
     2821 attttcttta agaaattcct tttaaattgt taatactact gagtgcccca cttctctgaa
     2881 ttttctttaa cttctccctc ccctttttta tttgtatata cattcatata cgcttgttaa
     2941 tatcaaatca accgtagtct ctcttatgtc taattatata gaaagattgt tctttctata
     3001 taatttagac aatttaaaaa attaagattg cctccttctg tttcctttcc tattcatgta
     3061 t
//
LOCUS       DAGCYL010000212         3036 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.219, whole genome shotgun sequence.
ACCESSION   DAGCYL010000212 DAGCYL010000000
VERSION     DAGCYL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.219"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(171..1481)
                     /locus_tag="MB216_002585"
     CDS             complement(171..1481)
                     /locus_tag="MB216_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBT4527363.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1493..2911)
                     /locus_tag="MB216_002586"
     CDS             complement(1493..2911)
                     /locus_tag="MB216_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4527364.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          952 a          624 c          469 g          991 t
ORIGIN      
        1 tggttctttt taacgactta gttatcttat cacgtctaaa aacatgtgtc aactcttttt
       61 tggaattttc ataaaatgtt tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa
      121 aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt tcaacgattg
      181 tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca gcttttgaaa
      241 cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg gcaatgagaa
      301 aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc tcgggaagga
      361 agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc tttgactttc
      421 gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc tggcatttga
      481 gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat ccaagaaaaa
      541 ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat cactccccct
      601 ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatc gatatcgtga
      661 atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc gtatcctgaa
      721 cggattttac atctagcaac ctcgattctt gatgattttc gattgactct tgcaccattg
      781 ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc acattcatag
      841 acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc atcattgttg
      901 cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa gttccaaaaa
      961 cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata cagtaaacaa
     1021 gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg atcgtattca
     1081 gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg gtctccttga
     1141 ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac attcaaaatc
     1201 gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg aaatgcttcg
     1261 atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact cttatctttt
     1321 aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa gtgttctttc
     1381 gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt
     1441 ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct ccctatcttt
     1501 cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc agtgggtaac
     1561 ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag tttgccatcg
     1621 tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta gacgattggt
     1681 aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt cctacttttc
     1741 tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc ttatattgat
     1801 gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa ccagatccta
     1861 aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga aggaaataca
     1921 tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc tttttaccaa
     1981 ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg taagtaaata
     2041 aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa taagtcatat
     2101 tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact aaaataatca
     2161 gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt tcgtttttga
     2221 caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca aatgctattg
     2281 cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt tgtccactct
     2341 tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg ccaatcgcag
     2401 aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag ctaattgctg
     2461 ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa acaatgtatg
     2521 aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca
     2581 aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga ataaatcggc
     2641 cagtttctgt taactctgag acatcacata atcttccaac catcacatca gcaaacgcat
     2701 ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg ccaagaacat
     2761 tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag ttacctagat
     2821 caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct ttttttattg
     2881 tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct tctagtaaac
     2941 gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg gctttttatt
     3001 gcattatctt gacagataaa aaacaaaaaa taatct
//
LOCUS       DAGCYL010000213         3007 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.221, whole genome shotgun sequence.
ACCESSION   DAGCYL010000213 DAGCYL010000000
VERSION     DAGCYL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.221"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(276..692)
                     /locus_tag="MB216_002587"
     CDS             complement(276..692)
                     /locus_tag="MB216_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527365.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(695..979)
                     /locus_tag="MB216_002588"
     CDS             complement(695..979)
                     /locus_tag="MB216_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527366.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(1679..1774)
                     /locus_tag="MB216_002589"
     CDS             complement(1679..1774)
                     /locus_tag="MB216_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBT4527367.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            2427..>3007
                     /locus_tag="MB216_002590"
     CDS             2427..>3007
                     /locus_tag="MB216_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4527368.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALREL"
BASE COUNT          957 a          478 c          565 g         1007 t
ORIGIN      
        1 aaaaaaatac ttcaatgatc ttaaagtcac aaaaataaaa gcaaaaaagg gcaataaaat
       61 cgccaaatta gaatttactt tttcaagcct aacgtcagat ttgccaaaga tttctctgcg
      121 taactggtta gaagaataaa taaaaaagaa ctcgcccccc ttacattaag caagtagaca
      181 agtcccttac aaccgactaa gattgctagt acattctagc atattgtatg gggcttctaa
      241 ataaaaaaca gataggaata actcctatct gttttttatc gttttagaaa atttgttggg
      301 tcgaaatttc tagaagtgac tgtttgactg gtttcttttt gatgattctt tggtcgattt
      361 cgatttgatg gatttagttt agcttcgatt ttttctttca cttcttgata tactttagaa
      421 tttgtgccaa taagaacgcc tgtatctttt agatataaat cagcaaattc tgcgatgatt
      481 tcagtcatga tggaaatttc ttttcccata gcatttagtt ttgcctgatt gctggtagtg
      541 gtcgtgttgg tatcgggttc ataactcata cataaaaagc gaatggcttc cgagtagtta
      601 ttgatatctg gatttttttc gatgatggaa tctaaaacgt caacatgact agggtacaaa
      661 gtaacatgtt tttggacaga agtttttctc atggttatat cattcctccg ataagcattt
      721 caatgatttg attatttgtg tagacatact cgcttaatat ttgagtattt tctgtttgga
      781 tagcaataaa tttatcgagc aataaatcga attggtcttt tttgtaagtc gtaagttgac
      841 ggtcaaaatc actttcgatt aaaactttta gatatttatt tcgactccat ttttctcctg
      901 ttttattcgt gagttcttgg caacgtttgt ctattgttgc aacaaacttt ggatcaatat
      961 tccgaactaa aatgtccata aaaaatctcc ttttgcaatt atgtataatt tatgtatacc
     1021 atacaaatac tatatttgta tagctgtttt ttgccaaaat gctatacata agctacccac
     1081 gatggcgaaa aagtgatatc aaattttttt ctgagaaacg tgataggcac agcctatcta
     1141 cccacttttt ttagaaagtg ggtacacgtt tccttatgct ctttaaaatt cgggggggag
     1201 tgctgcacgt gtaatcgata accaaattta acagacgaaa aattgttgga atataaaaat
     1261 atgacacccg aaagaaaacg atcgttggaa aaattaatgc aactagtgaa cgaagacggc
     1321 ggaatttcag acgcagcttt tgacgcatgg agcgaatttc atggagaata taattaagtg
     1381 ggtttaaatg gctctaaaat cgcttctaag agttttttag cttggaccaa tgaaatacac
     1441 caaaaactaa ataaaacgtc ttaaatcgaa aaatacgagc tctcagagcg actttatttg
     1501 taaatgttgc atataagaaa taattacgga ttttagactt ttgtaggata tttttgaaat
     1561 aaatatttat ttctgttata atttgattgc aaataagtga acacgaaaca cacgaaatcc
     1621 ccagagaggt cggattctct ggggcttttt ttgtgggctg agtcgcctta attaagtttt
     1681 acttcttgtt tcggttattc aaccagtaac tgaacaaagc gcttaaaagc ccaacaagaa
     1741 gggagcaaat aaagttagtg aacacgaaac acacctcctt ccagtcgcaa gactgtcgga
     1801 taggcgacaa tcaattatac aataaaaaag caaaaataag cctaagtttt ttattgcctt
     1861 ggggttgtta gggcttgccc tgaccgcctg taaggcgctt tagaacgaag gacatagttt
     1921 ttatcgaatc ttgtcttaga acggcttaga cgagctctca ggacgccaca aaggagtccc
     1981 taatgaatta tttttggcga aggagcctaa aggcgactgc tgcaagtaaa atgtgagagc
     2041 gtagcgatca ctccttctta cagcgaccat agggagctag attggcagtt taatatcatg
     2101 cgcgaacctg tttattttaa aaagtgggtg attttttggc gggaaatttt gctcgcgcgc
     2161 gcgcgtttat tttatttatt ttttaaagat tttttagaac cctttttagg gggcagttga
     2221 gccttactct cccaacggat acagaagggt actaacaact ttttatctga gtactaacaa
     2281 ctttttatct gagtactaac aactttttat ctgagtacta acaacttttt atctgagtac
     2341 taatatcctt ttgtataata acaaccaacc tatttacgag ttggttgtta aaggttatta
     2401 tttatttgta aggggggaag aaaggaatgg atgaaaaaac ggaattaaca gtcaaatatc
     2461 aaaatgaatt gaatttagta ccgctgaaaa atttcaatgc taagcagatg gatttatttt
     2521 ttgcattatg cgctcgtatg aaagatcgtg gtttgaaaaa agttagattt tctttcgagg
     2581 aattaaaaga gttaagtgat tataagatga ctgctacaaa agcctttgtc ggtgatttag
     2641 aaaagctcta taaagatatg cttaatctta cttaccgtac tgaagatgag gattctattc
     2701 gctactttgt tttgtttact ggttttgaaa tcaataaaac aaaaaagttt gttgaagtca
     2761 gtgttaatcc ggacctagaa tatattatta atggtttaac agctgaattt agtagatttg
     2821 aattatctgc tttcacgtcg ttgcgttcta cgtatgcaaa aactctattc cgactgctta
     2881 tgcagtaccg ttctactggt tattatgtag ttcgtattga agagtttagg agcttaatgg
     2941 atatcccaga atactatcaa atgggtaaca ttgatcaaaa agtattaaat cctgcattac
     3001 gagaact
//
LOCUS       DAGCYL010000214         2998 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.222, whole genome shotgun sequence.
ACCESSION   DAGCYL010000214 DAGCYL010000000
VERSION     DAGCYL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.222"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(261..623)
                     /locus_tag="MB216_002591"
     CDS             complement(261..623)
                     /locus_tag="MB216_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527369.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(697..1077)
                     /locus_tag="MB216_002592"
     CDS             complement(697..1077)
                     /locus_tag="MB216_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBT4527370.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1141..2334
                     /locus_tag="MB216_002593"
     CDS             1141..2334
                     /locus_tag="MB216_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBT4527371.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2411..2995)
                     /locus_tag="MB216_002594"
     CDS             complement(2411..2995)
                     /locus_tag="MB216_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4527372.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          934 a          578 c          495 g          991 t
ORIGIN      
        1 ataacatcca cttcagaatc aacgctgtta aatttatcat gtatttcttc cttggtcaac
       61 tataaattaa gcattacttt gtgaaatgat ttttcgatca attattatta ttcgttatac
      121 aaccgttttc aacatatttt gaaaataaaa tgcactaaaa aactatcttt agcagaacat
      181 ttcattgtat tttttggacc aaacaataaa aaaagaagtt tgaaaaaaat tcaaacttct
      241 ttgttcgctt aatctgtatt ttagaagaaa ggcactccct ctacttcgta agttccaaga
      301 acagagcgca tttgacctgg ttcttcccaa atatatcctt cgcctaattc ttctctttga
      361 ttcttttgtt cagtttcaaa gacaaacaac ggaacatttt cctctttaat atgtgtattt
      421 gtcaattcca tcaaactaat cttgctaaca tcgatatgaa ctatttcttt taagaaatta
      481 agtatattcg ctaaaccagt ctttccttca gaaagttccg ctaacgcaaa ttccaacttg
      541 tcatcaattg aatggaccaa aaatttttta gtaccatcct ccaaatgcaa cataattgta
      601 ccagcaactc ttttgcttgc caaaatttcc acctcgatca acttgttcta tggcttacat
      661 aaaaattcta gcacattttc agaaaaaata cactttttat ttcttatttt ctttccgttc
      721 tagttcagat aatgcttctt ctatccaaat aggtaactgc tccgcaatag tggtaaatcc
      781 gatcttacga tttttattcg tgaaatacct ttttctgcga tatggaacag ctggatttgt
      841 tttttctaac gttcttaaag ataaactgat ttttttggaa tattcatcaa tgtcgatgat
      901 ctgaactgtt actggctgtc cgacttttaa caatgaatgg atatttttcg tataaccgga
      961 ttgtacttct gaaacatgaa taagtccttg cgtctcgtta tcaagagaaa cgaatgcgcc
     1021 ataaggctgt atccctgtca cttttccttc aattatttgt ccaattttat aagtcatcta
     1081 cttccctctc ttcgcattta tcattatact gtaaatagct caaaaaaaga agcgacgaaa
     1141 atgactggag gtcttaagat gatcgaattt gataaacaga tttcaagaaa agatacagaa
     1201 agtgtaaaat gggacgcaat tgctgagaca tatcaaatgg atgacttact tcctttgtgg
     1261 gtagcagata tggacttctt gtctcccggt ggggtagcta aagcgtttag tgaatacatc
     1321 aaacatggaa tctttggtta tgccactctg tctgataaac tatatcaggc tatcattcat
     1381 tgggaacgcc aacaccatgc tgtggagttg acgaaagaaa atatcgtttt tacaagtggt
     1441 gtgttaacca gtttagctgc agcagtacaa acttttacaa aaccaggcga ttctattttg
     1501 attcatgacc cagtttatcc tccattttcc tctattatag aaacaaatca acgtcacatc
     1561 gttcgaagct ctttgctaga aaaaaataat catttcatca tggacttgtc tgacatggag
     1621 aaaaaaatcg aagaaaacaa tgtaaaagca atgattttat gcaatcctca caatcctgga
     1681 gggcgtgttt ggagcaaaga agaattgaaa cagttaagcg aattgtgttt aaaacatcaa
     1741 gtgattctgt ttagtgacga gatccatcaa gatttaactt tattcgatca tacatttact
     1801 tccatgctaa cgattgatcc taatttagat gatctactag ttgcttttac ttccgcaacc
     1861 aagactttca atctagctgc aatcaaaaat tcaatggtat ttatcaaaaa tccagagatg
     1921 aaagctgatt ttgaaaaaca tttagtgatg aaccaacagc acgaaatcaa cacttttgga
     1981 ctgattggaa cccaagcggc ttatgagacg ggtggagaat ggttaagcca actgattcct
     2041 tatctagaaa aaaatgttca aacagttaat cagttttttg aacagtatct tccgaaagtg
     2101 agaataatga accctgaagg aacttactta atgtggttag attttaccgc ctatatttca
     2161 gacgaccaag aattggagaa cactcttgtt cggaaaggaa aagtcgtatt aaatcctggc
     2221 atcacgtttg gtccttccgg tcatgggcat atgcgcttga atattgcttg tccagaagaa
     2281 actttactgg aaggacttaa tcgaatcaag tatgctttag agacaatcga ttaaatcatt
     2341 tattcaaaat aaaaggacga gaccttacta ggtttcgtcc tttttcataa ttactccgta
     2401 ccataccaaa ctattctgaa atttcaattg tttcaatcac tacatcatgt acaggtcggt
     2461 cttgtggacc acgttgtaca tttgcgattt catctactgt gtccatccct tctagtacat
     2521 gtccaaagac tgtatgtcgg aaatctagcc aaggagttcc gccaccttta tacgcttcaa
     2581 tgatttctga tgggaatcca gcaccttcta gttgtccaag catattagct ggcacatttg
     2641 tgttatttac aataaagaat tggcttccgt ttgtgtttgg acctgaattt gccatagata
     2701 aagctccgcg aaggttgaac agttcacgac tgaattcatc ttcaaaactt tcgccataaa
     2761 tactttcgcc acccattcct gttccagttg gatcgccgcc ttggatcatg aaatcaggaa
     2821 taacgcggtg aaaaatcacg ccatcataat aacctttttt ggaaagttca atgaaatttt
     2881 gaaccgtctt cggtgctagt tcagggaata attgaacagt aatgtcacca cggtttgttt
     2941 tgataacagc ttttggtcct ttttcacttt ttaaatctag ttgtgggaat gccatatt
//
LOCUS       DAGCYL010000215         2945 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.223, whole genome shotgun sequence.
ACCESSION   DAGCYL010000215 DAGCYL010000000
VERSION     DAGCYL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.223"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            6..977
                     /locus_tag="MB216_002595"
     CDS             6..977
                     /locus_tag="MB216_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HBT4527373.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            970..1998
                     /gene="vanB"
                     /locus_tag="MB216_002596"
     CDS             970..1998
                     /gene="vanB"
                     /locus_tag="MB216_002596"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HBT4527374.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2004..2612
                     /locus_tag="MB216_002597"
     CDS             2004..2612
                     /locus_tag="MB216_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HBT4527375.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(2678..2851)
                     /locus_tag="MB216_002598"
     CDS             complement(2678..2851)
                     /locus_tag="MB216_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527376.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT          780 a          625 c          812 g          728 t
ORIGIN      
        1 aaccaatgag aaaaagtgtg ggcattactg tttttggatg cgaacaggat gaggcaaatg
       61 ctttccgcgc tttatcgccg gattttcata ttatccctac gctgattagc gatgcgatat
      121 cggcagacaa cgcaaaattg gccgctggca atcaatgcgt tagcgtaggc cataagtccg
      181 aggtttccga ggcgacaatt cttgcgctga gaaaggtcgg ggtaaaatac atttctaccc
      241 gcagcatcgg ctgcgatcac attgatacga ctgccgccga gagaatggga atctcggttg
      301 gcacggttgc gtattcgccg gacagcgttg cggattatgc tttgatgctg atgctgatgg
      361 ccatacgggg tgcaaaaccc accatgcacg ccgtggcgca acaagatttc agattggatc
      421 gtatccgggg gaaagaactg ggggatatga ctgtgggagt tattggaacc ggccatatcg
      481 ggcaagcggt cgtcaaaagg ctgcggggat ttggatgcca tgtgctggcc tatgataaca
      541 gccgaaaaat ggatgcagat tatgtccagc ttgatgagct tctaaaaaac agcgatattg
      601 ttacgctcca tgtgccgctt tgtgcggata cccgccatct gatcggtcag aagcaaattg
      661 gagagatgaa gcaaggcgca tttttaatca acaccgcgcg cggagcgctt gtcgataccg
      721 gggcgctggt ggaggcgttg gaaagcggaa agctgggtgg tgcggcactg gatgtgttgg
      781 agggcgagga tcagtttgtt tataccgatt gctcgcagaa agtgcttgac catccctttt
      841 tgtcgcagct tttaaggatg ccgaatgtga tcatcacacc ccatacggcg tactacactg
      901 agcgtgtgct gcaggatact acagaaaaaa caatcaggaa ttgtctcaat tttgaaagga
      961 gtttacagca tgaatagaat aaaagtcgca atcatcttcg gcggttgctc ggaggaacat
     1021 gatgtgtcgg taaaatccgc aatagaaatt gctgcgaaca ttgatacgga aaaattcgat
     1081 ccgcactaca tcggaattac aaaaaacggt gtatggaagc tatgcaagaa gccatgtacg
     1141 gaatgggaag ccgacagtct ccccgccata ctctccccgg ataggaaaac gcatgggctg
     1201 cttgtcatga aagaaagcga atacgaaaca cggcgtattg atgtggcttt cccggttttg
     1261 catggcaaat gcggggagga tggtgcgata caggggctgt ttgtattgtc tggtatcccc
     1321 tatgtgggct gtgatattca aagctccgca gcttgcatgg acaaatcact ggcctacatt
     1381 cttacaaaaa atgcgggcat cgccgttccc gaatttcaaa ttattgataa aggtgacaag
     1441 ccggaggcgg gtgcgcttac ctaccctgtc tttgtgaagc cggcacggtc aggttcgtcc
     1501 tttggcgtaa ccaaagtaaa cggtacggaa gaacttaacg ctgcgataga agcggcagga
     1561 caatatgatg gaaaaatctt aattgagcaa gcgatttcgg gctgtgaggt cgggtgtgcg
     1621 gtcatgggga acgaggatga tttgattgtc ggcgaagtgg atcaaatccg gctgagccac
     1681 ggtatcttcc gcatccatca ggaaaacgag ccggaaaaag gctcagaaaa tgcgatgatt
     1741 acagttcacg cagacattcc ggtcgaggaa cgaaatcggg tgcaggaaac ggcaaagaaa
     1801 gtatatcggg tgcttggatg cagagggctt gcccgtgttg atcttttttt gcaggaggat
     1861 ggcggcatcg ttctaaatga ggtcaatacc atgcccggtt ttacatcgta cagccgctac
     1921 ccacgtatgg tggccgccgc aggaatcacg cttcctgcac tgattgacag cctgattaca
     1981 ttggcgttaa agaggtgata agtatggaaa atggtttttt gtttttagat gaaatgttgc
     2041 atggcgtccg ttgggatgcc aagtacgcca catgggataa cttcacagga aaaccggtgg
     2101 atgggtatga ggtgaatcgc atcatcggca caaaggccgt ggcgtttgct ctgcgcgagg
     2161 cacaaatcca tgcggctgcg cttggctatg gcttgctttt atgggatgga tatcggccaa
     2221 gaactgcggt ggactgcttc ctgcgttggg cagcgcaacc ggaggacaat ctcacaaaag
     2281 aaaaatttta ccccaatata gagcgagccg agttgattac aaagggttat gtggcctcac
     2341 aatccagcca tagccgtgga agcgcaattg atcttacgct ctaccacctg gatacagggg
     2401 aacttgtttc aatgggaagt aacttcgatt ttatggacga acggtcgcat catacagcaa
     2461 aagggatagg ggatgcagag gcacaaaatc gaagatgctt gcgtaaaatc atggaaagca
     2521 gcggatttca gtcttatcgc tttgaatggt ggcactataa gttgattgat gagccatacc
     2581 ccgataccta ttttaatttt gccgtttcat aatgaaagta ttggttttgt atcagatttt
     2641 ggatttgata aaatctatgc tggtcatgga caatctttta ctggaaagtg gttaaagcat
     2701 cttttgtaat acattgcaaa ttatagtatt caatctgccc agcggcagaa aagaaaaaaa
     2761 ccgctgttgg gcagaccttt cccacgccca ttttgtattt tgctggcggc ggttttgaga
     2821 aatcaaggcc gccgttcttt tctggcagca aaaggtgtgg cggcccatag gaggatgccg
     2881 ctatgctgtt ttgctattgc catgctctaa tccacttcca ccactcaaaa aagccttgcc
     2941 cctcc
//
LOCUS       DAGCYL010000216         2937 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.224, whole genome shotgun sequence.
ACCESSION   DAGCYL010000216 DAGCYL010000000
VERSION     DAGCYL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.224"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(212..538)
                     /locus_tag="MB216_002599"
     CDS             complement(212..538)
                     /locus_tag="MB216_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527377.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(550..750)
                     /locus_tag="MB216_002600"
     CDS             complement(550..750)
                     /locus_tag="MB216_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4527378.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            873..1475
                     /locus_tag="MB216_002601"
     CDS             873..1475
                     /locus_tag="MB216_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4527379.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1530..2696
                     /locus_tag="MB216_002602"
     CDS             1530..2696
                     /locus_tag="MB216_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4527380.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
BASE COUNT         1030 a          492 c          428 g          987 t
ORIGIN      
        1 atatcagaat tagaatagcc ttgcttttca actaagcttt caacttcttt ttcattcatt
       61 gtcacacctg cttctgattt tatttgctat aatagattca aataaagttt tctcagaact
      121 ttattattga gtgttccccc gatctgcaaa ctgacgggaa cactcttttt aattttttct
      181 acttcatcaa atttaaaaaa cgaggtgttt tttaaatttg atgactcaat atccattttt
      241 tgcattcttc ttttgaaaac aactccattt tttctaataa aataactggc aatcccattc
      301 ttttaaattt caaaaaagtt gtatatgata cattcaaata tttagccatc tcttttcctt
      361 ttagaaatgg tctttctaat ccggtatcaa ttcttgcttg atcaatagcc tcttttgtca
      421 tttcaaaaac ataatttttc aattgattcg tttgttgttt atctaacatt actgtaattc
      481 ctgcatccat tatttttctc ctctcatttt ctatttcttc aatcagccaa ctcgccaagt
      541 tgtatttatt tagattttca aatcattaag taaccaattt accaactttt gataagtaga
      601 tgctctaata ctttctttgc tcccattttc gattttgaca tatgttttcc gatttatacc
      661 tatcaatttt gctgcatcgt ttttagtaag tataagatcg gctctatgtc ttcttaagct
      721 tctaatgaat tgatcattaa gagttatcat tcagcgcctc ctttcaaatt attcactttg
      781 tggtttttat cttaataagc acattttaaa ccacaaaacg agtaatatca attaaaatat
      841 tactcaattt gtggttttca tgctataata gtttggaatt tactataggg ggcgttactt
      901 tgaatagaat aaaattttat attgattcca gtaaaaagac cattaaacaa attagtacag
      961 aaactggtat taactataca actcttacga actattaccg tggtgagcga aatcctagaa
     1021 atactgaaac atggaatatc cttgctgatt atttccatgt tcctgtaagt tatttgatgg
     1081 gattagacga aacaaattcc atagaacaac ctaatcttga tagaataaaa actgctcgtt
     1141 tagaaaaaaa ggacaaaata gtcaagaata ttgccagtct tgactcagat tattttactg
     1201 aattatctaa tctatacttt tccatgactt ctttacttga agtcaaactt caagaagatg
     1261 actccgactt tcttttttta aaaagtattc tagaggactc ctttgaatac acgattctat
     1321 ttgccaaaca aaagaagttt attaataaag ctttaaactt aaatatcgat gaaagaaaag
     1381 agttagaatc tgagttattt ggcaacttaa cccaagtaca ggttaacaat ctaaaaagag
     1441 agataacagc tcgattaata tcaaaacttc aataaaacta attattcaat cagccaactc
     1501 gccaagttat cagattggag agattagtta tgtcaataca aaagtacaca acaaaaagcg
     1561 gtgcacgata tagtgtccgc ttgtatgttc gtaaagatga aaatggaaag caagaatact
     1621 atgtaaaaaa agggtttaaa actgaaaaag aagccaagtt acacgaagca agaaaaaagg
     1681 tcgagattga agataccggc tatcataagc cttctaatga cttgtttata acagtgtatg
     1741 aagaatggtt gccttcttat aggaatcgtg tacaagaaac gacatatcaa cgtacaaaag
     1801 accttttccg cttgcatata ctaccaaaat tcgggaataa gtccatttct aagattactc
     1861 ctgttcactg tcagaatgca gtcaatgaat gggctttaaa attcaagaat atcaaacagc
     1921 ttaagtctta cagctctcaa atatttgagt atgccatttt tgcagagcta ttacaccgaa
     1981 atcctatggc aaatacaaat ctaccaaagc gggaaaagaa agaaaatgaa aattactttt
     2041 cattagaaga actaaaagct ttttgggata ttctcaataa agaagagcct ttaaaacata
     2101 ttttaatctt ccagcttttg attactactg gtatacgtaa aggagagcta gctgctcttc
     2161 attggtctga catttatttt gaagaacagc taatgtatgt tagaaagagc tatgcaacta
     2221 ttcgaaatga agagaagaat gcaagaagaa aaactgtcag gattcaaaaa aatacaaaaa
     2281 atatatcttc cgaacgaata ttaccaattg atgcacaaac tattgaaatg ttaaaaatct
     2341 ggaaaaaaga acaagctcga gagcttttgc agtttggcat aaatacaaat acaaaaaatc
     2401 aacttatctt tacctatgta aatgcagacg gtgacattaa tcaacctttg catgcggact
     2461 attcaaataa tatattgaaa cgtttagaga agaaatataa atttaagcac gttactattc
     2521 atggattccg gcacactcac gctactcttc tactagaagg tggagcaagt atcaaagaaa
     2581 cacaagatcg attgggtcat aaaaatgctg aaactaccct taacacttat tctcatgtta
     2641 ctgaaaaagc tcaacgaaat gcgttagaaa aattcattgc ttacactggt ttataatatt
     2701 gtcattacct ttctcattac cagaacgaaa aaaagacacc ttctcgcaat gagaaagtgt
     2761 ctgaaatcgt tggtataaca acgattaacg ttttgaaaac tgtgaagctt tacgtgcttt
     2821 cttaagacct ggttttttct tcgtatcatt gaataagttt gaaaagcctt gatttataaa
     2881 ggtttttccg taaatatatt ttgttatatt tgattaaatt tgaaatgttt gtgttct
//
LOCUS       DAGCYL010000217         2918 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.225, whole genome shotgun sequence.
ACCESSION   DAGCYL010000217 DAGCYL010000000
VERSION     DAGCYL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.225"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            46..864
                     /locus_tag="MB216_002603"
     CDS             46..864
                     /locus_tag="MB216_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBT4527381.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            868..1164
                     /locus_tag="MB216_002604"
     CDS             868..1164
                     /locus_tag="MB216_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527382.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(1346..1699)
                     /locus_tag="MB216_002605"
     CDS             complement(1346..1699)
                     /locus_tag="MB216_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527383.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(1719..2774)
                     /locus_tag="MB216_002606"
     CDS             complement(1719..2774)
                     /locus_tag="MB216_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBT4527384.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
BASE COUNT          945 a          453 c          549 g          971 t
ORIGIN      
        1 tagtgtataa tggagttaca aaaggttaat aagggaggtt taatcatgga aaagaagttg
       61 tcaatcatta ctggaaacat tgaaaaaggt ggaacaggta aaacaacaac aatctacaat
      121 ctaggtggat atgctgtcga aaatttaggt atgaaggtgc tgtatattga tgaagataag
      181 tcgcaaaacc tatcaaaacg atttcctgat gctttcagta aagcgaaaag agagaataca
      241 atcaatcatt tatatcttca tggatcagct gaacctttag ctgtaaatga gaacatggat
      301 attttagtag caggtgaaga tttaaaacaa gttgagatgc aaatgcgtga tatgcctaac
      361 aacagaaccc tactgtttag atggatggtt gttaactatg aacagcttaa agagaagtat
      421 gatctaattc tgattgatac tcataatgat gcaggtctac tagctcaaaa tgcttgggct
      481 gtatctgatc ttgtcgtagg catttctgat ccatctaaag atggttttga agctttgctg
      541 aagcttggta aagatattga tgcactagca aaacagttga ttgatattaa gactggtgaa
      601 acactgatgg aaactaaata cgctacgatc ggtagcagga tcaaacataa tactaattca
      661 agtcgtgagt tcctagaact ggtaccaacg ttatctaatt atcttggata tgtacaagag
      721 aaggaattgc ttaatgcaac tagcttagaa ggtaagaata ttgctgagtt cgctaaggat
      781 aagaatatct atcgtaagaa taaggatttc tttgaaagca cgtttacgct atacaacaaa
      841 ataattgata cggcacaaga ataaggagtg attgaagtga aagaaaatag atttaaaaaa
      901 ttcgctgaag ctgaagtaga agaagttcct gaagtggttg aagcagttga agaacctaaa
      961 caaacaccta aagaaacaac aagttctttg gtagctaaac taaaaagtga tagttctgaa
     1021 aagaagaaaa attatcaatt cacgcttcaa ccatcgcaaa gaaagaaact atctaaaatc
     1081 agcgaaaagt taggaattcg atcagattca gctttacttg gtgaaatgat agataacttg
     1141 tatgatgaag tttttggtga gtgatcagtt taaagctgat cacttttttt attggaataa
     1201 gcggttaggt atagcttaac taaatttaat atacgtgttt atggatatat acgtatacat
     1261 gtttatatac atgtatacgt atatgtttat gtttatattt gtacataata aaaaggctag
     1321 gtattgtaac ctcgcctttt tatttttaaa gtaagtcttt gttacttggt gcattgaacc
     1381 ctttcagcat tggcttttct ttctttgctg aatgatcgga tgtaaccgtt gaaggatgct
     1441 cttgttgaat agggttatga tcagttggtt tattctttac ttctgctact ttgctatcgt
     1501 ttctatcgtt gcctgcttca ataattgtaa cttcattgtt ctttacacgc cgatataggt
     1561 tcaaagcttt tttgatcgtc tgagagtatt taacgcctag tgtttcatat tcggattcaa
     1621 gaaactctgc taaaggtaac tcgctaacct tttctctatt gattataact acataacgct
     1681 ctgattttcc cttattcatg ttgatctcct tcgctgtttt attgagttaa accaaacttg
     1741 taaaagcctt ttacattaga aaattcactg tcttcaacaa acaaggtatg cggaatttta
     1801 tcttttacaa gttgtttgtc gataatagaa gctccaccac cagtaaagac aactttatct
     1861 aaggttgtga agtttttgaa tgtagctaaa acttctgcaa caaccatatt ggtgtatgtt
     1921 tttatttctc tttcaactac ttctgaaata tcaaaacttt catttccgct tggcttaaat
     1981 agatatacgc ctttttcttt gccttctcgg ataatctttt caagttggaa agcattagga
     2041 ctgatcggaa ggtcaagcat atttacgata cgattatata aagtaaaaat gccggtatcc
     2101 ttttgttcac gattgttttc gtcaatttca aaattttgga caacatcaaa aagtttcgtg
     2161 ccaccgccaa tatcaacaaa accgatcttc tgactgatca atgtttcatc ttgtacacta
     2221 ccatcatcag caagtaggag gttatatagt gttccaatcg gctgtggcag tacccaaacg
     2281 gtatctacgt ttatattata agctttaccg tcaacttcaa ctgaatgttg tcgtgtggct
     2341 tgttgcatca cgtatttgac gattgtttcg gtatagtcgg atgaaggcag acctaaaact
     2401 agattagcgg ttaaaacgtc atcagattga tcttctaaac gtgctaggtt agctaaagag
     2461 aaagatagga tattttggta ggtttcctgc ttgtatctgc tgatcccaaa acctagtgat
     2521 tctatcacgt agtcatcttt tcctagctcg tgtatatcct tcccaaagta atagctttca
     2581 gtagtgttta ctaggctata ttcagaaacg tttagtgatc gttttaatga ggaacctata
     2641 ttagaaaaac gtcctgaatt aatgatattt ttgttcaaaa tacgagaggg aagtgagagt
     2701 ggagaaacgt gtgtactcat ttcttttact gatttattcc ctaagtcaac ggaaaacaat
     2761 aatggttctt tcatggtatg acctctttct gtttgtgttt atggtatttc ggtacgaaaa
     2821 tagtttacac ctatttacta ttagttgcaa ctgtttataa gaaaaaaatg caatcataaa
     2881 agtctaatta attataataa agtgtaaata aaaaggtg
//
LOCUS       DAGCYL010000218         2828 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.226, whole genome shotgun sequence.
ACCESSION   DAGCYL010000218 DAGCYL010000000
VERSION     DAGCYL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.226"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            65..865
                     /locus_tag="MB216_002607"
     CDS             65..865
                     /locus_tag="MB216_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBT4527385.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            881..1759
                     /locus_tag="MB216_002608"
     CDS             881..1759
                     /locus_tag="MB216_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBT4527386.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            1772..2752
                     /locus_tag="MB216_002609"
     CDS             1772..2752
                     /locus_tag="MB216_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBT4527387.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
BASE COUNT          958 a          470 c          596 g          804 t
ORIGIN      
        1 ataatcaaaa agaatttata gttgttaccg tagtagctac taaaaataaa gtgaggatta
       61 atcaatggaa acgattattc aaatcatgaa aaaatatgat ttttcagaga tggagacaag
      121 agtgtatacc acactattag aaaaaggtaa tcttacagga tatgaagtaa gtaaaatctc
      181 aggtgtgcca cgctcgaaag tatataatat tttggaaaaa ctattaaaaa agaatttgat
      241 tgtcgttaat aaatcagaac ctaaacttta tcatgccatc tcggcaaatg agtttttaga
      301 gaaattagaa aaaagtgtta aaaatgatct ttcttttctt accaagaatc tagggatgat
      361 caaagaaaaa gatgaagaag aaatgctgtg gaaagttgat ggtatcgagt atgttctgga
      421 taaagtagag catttggtga aaaacgcaaa agaaagtttg ctcattcaag tttggcatga
      481 gaatttcacg gattcactac taaaggcatt gcagcaagct gaaaaaagag ttgataagtt
      541 tgtactgatt ttattcagtt ctacccatga atatgatctt cccttagaaa agtattatat
      601 tcatggtttt gaaacagaca aacttgctga ctttggagca agatggatca acatcgtcgc
      661 cgatgagcaa gaagtcgtgt ttggtaccat taacgaagaa ctgcaatcaa ccgatgtcac
      721 ttggacaaaa aaccacgcca tggtcaatct agcaaaagaa tatgtcaaac atgatgcata
      781 tacactaaaa gtgattgcag aatctcctga agaacttaaa gcaaaatatg gtgagggctt
      841 tgagggaatc agaaagattt actagtagga ggaatttttt atgattcgga atattttgct
      901 gacactaatt gtcgcaatca atctatattt tattatttac tttctaagag accttttcaa
      961 aaacaaacaa aatttcaaag aagaaccagg ggatatgcgt ctgttgccct tcacgtcatt
     1021 tatcatcttc tttctttcaa catttggcgt ttccgacttt gctatcggaa ccgtccttta
     1081 tccaaagttg aaatgggtca gcatgaaaaa actacccgga acgttgaata cccaatgtgt
     1141 agtaccggta gccgtcatgg cattgtctta catcactgcc atcaacgtag ggatcaagac
     1201 actagctgtt tgtattatct gccaaattat cggtgcatat ttaggaccaa gatttgtcgt
     1261 gaagttacca gagaaaacga tcaaactgtt tgtcggcata ggattggtca ttgctgcatt
     1321 gttgattttt gcaggacaaa tgaactggat tccatcaaac ggaacagcca gtgaattgta
     1381 tggcggaaaa ttaatcttgg caggattttt actctttgtc tatggtgcat tgaacaatat
     1441 cggcattggt tcttatgcgt taacgatggt aactgtctac ttgctaggct tgaatccagt
     1501 tgcagccttt cctattatga tgggggcttg taccttctca gtaccgatcg gcagtgttca
     1561 attcattaaa tttaatgaat acagcagaaa aatcacactt tttacttcaa ccttcggtgt
     1621 attgggggta ttggtcgctg tattcctagt caaatcttta gatacctata tcctgaaatg
     1681 gatcgtgatt ttggtcttac tatacagtgc atatacgatg ttgggcagtc aattaaaaaa
     1741 agttacagca gctaattaag gagcgattca aatgttattt cttaaaaaag aagatattat
     1801 caaaagtttt tcaatgagag aagccatcga tgcggacaaa aaagcactgt cattgtattc
     1861 agcaggaaag gcaagtgtgc ctcttcgcac caatatcgat gtgccaaaaa gcaacggaca
     1921 aagcttatat atgcctgctt atgtagaagg tggcgaaggc gcgctaggtg tcaaaatcgt
     1981 ttccgtgtat cctgaaaaca tcaagaaaaa cttaccaagt gtaccagcaa cgatgatcgt
     2041 attggatcca gaaacgggta tggtatccgc ttgtttggat ggtacttatt tgacacaatt
     2101 aagaacaggc gctgttcaag gagcagcaac agaacttctt gcaagagaag atgctaaaat
     2161 tggcgcgctg attggaaccg gcggacaagc acagtcacaa ttagaagcaa tgttgaccgt
     2221 tcgtaaatta gaagaagttc ggatttttga tattgatttt gagcgtgcca gccaatttgc
     2281 tgaaaaaatg atgcaacaat tttctgtaac gatgcgccca acaaaaacca gtcaagaatg
     2341 cgttgaagga gcagacatca ttacaagtgt gaccacttca aaacgtgcga cattctctgc
     2401 tgagtgggtc aaaaaaggtg cgcacatcaa tggagtcggt gcttacacac cggaaatgtg
     2461 tgaaattcca cgagaaataa tcaaagccgc agatgttgtt atttttgata ccatggacgg
     2521 tgttctaaaa gaagctgggg acttcatttc accgctgcaa gatgggtata ttcaaagaga
     2581 cagctatcat ggggaactag gccaactgat caatgaagaa ttgatcggtc ggacaagtgg
     2641 agaacaaatc acgatcttca aaacagttgg ttcggctgta ttagatgtgg tagttgcgac
     2701 agaaatcgtt aagaaagcta aagaaaataa tttgggaaaa ttgattttct aattaaaaag
     2761 gctgaaatta aaacctttat tattgcgttt aaaaaaagaa aacaaagaag tttgtaatca
     2821 aatggaaa
//
LOCUS       DAGCYL010000219         2731 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.227, whole genome shotgun sequence.
ACCESSION   DAGCYL010000219 DAGCYL010000000
VERSION     DAGCYL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.227"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1481
                     /locus_tag="MB216_002610"
     CDS             <1..1481
                     /locus_tag="MB216_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527388.1"
                     /translation="FYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKY
                     FLFFTVFLVLCWLPYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVH
                     DLPTDTINHGVFLFSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPI
                     YALNMQKDTLFSIACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVV
                     TGTGLICGLIILKQKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQ
                     ISRTIVMDGDLSKKDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFL
                     SLWARNLPSNIKIYTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLT
                     GLSGMKKIIDARDFFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATP
                     VAFSLRYVFVLALGLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            1468..2670
                     /locus_tag="MB216_002611"
     CDS             1468..2670
                     /locus_tag="MB216_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527389.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT          775 a          486 c          468 g         1002 t
ORIGIN      
        1 ttttttactt tcttgtttat ttctccattt ttgtgtattc ttttttattt ttacaatcga
       61 tttatttatc aatgccaaaa ctggaaacat tttttactgc tcaaaacaca caggctttct
      121 cttcaagaaa atatttttta ttctttactg tctttttagt tctgtgttgg cttccttatc
      181 tgttggcatt gtatcctgga gtggttttgc ctgattcgtt aagttcggtc gcccaatcta
      241 tgggtgatgt tcctataagc aatcatcatc cagtcttatt caccttactg gtcaagctat
      301 ttgttcatga tttaccaact gatacgatca atcacggtgt atttttattt tcgatttttc
      361 aaacattgat aacagcttca gcgataagtt attctctttg ctggctgtta aaaagaaaca
      421 tccatttttt tccggtactc attagtttat gctattttgc atttgcaccg gtttttccaa
      481 tctacgcctt gaacatgcaa aaagatactt tgttctctat cgcttgcttt ttattttcct
      541 tagcggtttt tgactttatt tttcaacgag aaaagccaac gattctcaat cagatttacg
      601 taatcatcct tgctttactg accacctatc taagaaataa tgggatttat gtagtaaccg
      661 gcacaggttt gatttgcggc ttgattattt tgaaacaaaa aaggtatcaa gcattctttc
      721 aagtggtgct gggttataca gtgattgctg gactactcat ccacccgttg atggctcgtt
      781 atgctattcc tacagaaact gctgaacaat taggtgttcc tttgcagcaa atttctagaa
      841 ctattgttat ggatggtgac ttgtctaaga aagataaagc attcatgaat cagctactcc
      901 cgataaaaga ttatgaagcg tattctccta gtcttgcaga ctcaatcaag tggcataaaa
      961 attttaacaa tgagtttttg aatacacata aaaaagaatt tctatcacta tgggcaagaa
     1021 atttaccaag taatataaaa atctatacag atgcttatat tttagaaacg ttcgggttct
     1081 gggtacctgg tacaaaaaat agttacggct ttttagatac tcgtgtaaac acgaataatt
     1141 atggtatgaa gcaagtagat ttgattgaac atcttactgg attgtcagga atgaaaaaaa
     1201 tcatcgatgc tcgtgatttc tttggttccg gcacattact gtggttagct atcggcagtg
     1261 cacttttagc aatctttaag aaaaaatatc ggtatttact cctttttctt cccggtatgc
     1321 ttgtttttgc tactattatg attgctactc ctgtggcttt tagtttacgg tatgtgtttg
     1381 ttttagcatt aggtctacct gtttatatcc tctctccttt tttactagat aaaagagaaa
     1441 aaatgaaaac agatatagga gatactcatg aaactattta aaaagatatt ttttgctttt
     1501 gttctcttga tcgctagcta ttgcgtacta ctgacaatta cttttttcat tccgcagcac
     1561 tctattgaat cgaatgcaga aaaatctcta cagatggttg aaaaagaagg aatgtatcct
     1621 tctatcaatc aaggaaatat gctaggcact cggttagata attttactga tcatttgatg
     1681 atacgaaaaa caaaagcaga tcctgaattg aactccttgg agaacgcgat gtctatggcg
     1741 gattatcccc gttattggca tggttatcag ctttttttac gacctttact gctagggatg
     1801 agtttgggga gtattcgaat gatttacgca gccattcttt ttctgctgat tggattgacg
     1861 agctactttt taaccaagtg tagcgacatc tatttaacga cagccttgtt gatttcgctt
     1921 gctataggaa atgctgcaac ctttttcttc agtatgcaat tttcaaatat ttggattttg
     1981 actttattgt ctgtattact atttttaaca aaaccacaac tctccaacac caaccaacaa
     2041 ttattcttgt atttctttgt cattggttct ttagctaact tttttgactt gttgacaaca
     2101 ccggtcatct cttgggggat acctgtgatc attatttatt atgtaacaaa taagtattta
     2161 atggataaac gcagttcatt gagcaaacaa gtaggcagtt ttgtttttac cggtatcttt
     2221 tggggattag gctatggttt gacatgggtg gctaaatgga tcctatcatc gatcatattg
     2281 aacaaaaaca ttgtaaaaga tgcaatcaac caaatcctct ttcgaacgga aggaaacgac
     2341 aaatacccgt tgcatcgatt agagatgctg aaaagcaata ttagattgat gtatccaaaa
     2401 gtagcaatat tattgctatt gattacttgt cttgtttttc tctattttgc ttatcgtaaa
     2461 cgaaatagct ggcgtccgtt ccaacttcta gttttgtttg ttttagctgc tgtaacgccg
     2521 tatatttggt acaatatttt agctaatcac tcacagattc attattggtt tacgtatcgt
     2581 acgcaaatca tcacaagttt tgctatactt agtgctttcg cctttttgat ccctcctgag
     2641 aaatcaaaag acgaactaaa ttacttttaa gaataccgat gtactaattc atttttaaac
     2701 ataaaaactc cagctgtcag agatagccgg a
//
LOCUS       DAGCYL010000220         2711 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.228, whole genome shotgun sequence.
ACCESSION   DAGCYL010000220 DAGCYL010000000
VERSION     DAGCYL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.228"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="MB216_002612"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="MB216_002612"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBT4527390.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1061 a          419 c          556 g          675 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt a
//
LOCUS       DAGCYL010000221         2710 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.229, whole genome shotgun sequence.
ACCESSION   DAGCYL010000221 DAGCYL010000000
VERSION     DAGCYL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.229"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            170..514
                     /locus_tag="MB216_002613"
     CDS             170..514
                     /locus_tag="MB216_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBT4527391.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="MB216_002614"
     CDS             515..934
                     /locus_tag="MB216_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBT4527392.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1026..1376)
                     /locus_tag="MB216_002615"
     CDS             complement(1026..1376)
                     /locus_tag="MB216_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527393.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="MB216_002616"
     CDS             complement(1342..2673)
                     /locus_tag="MB216_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBT4527394.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT         1003 a          492 c          522 g          693 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gcaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 atggacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt
//
LOCUS       DAGCYL010000222         2640 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.230, whole genome shotgun sequence.
ACCESSION   DAGCYL010000222 DAGCYL010000000
VERSION     DAGCYL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.230"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            144..494
                     /locus_tag="MB216_002617"
     CDS             144..494
                     /locus_tag="MB216_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4527395.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            908..1726
                     /locus_tag="MB216_002618"
     CDS             908..1726
                     /locus_tag="MB216_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBT4527396.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1799..2158)
                     /locus_tag="MB216_002619"
     CDS             complement(1799..2158)
                     /locus_tag="MB216_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBT4527397.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          877 a          382 c          474 g          907 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag
      181 caatcattcc tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa
      241 ttgtcggttt tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt
      301 ttattggtaa gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa
      361 aaagaattga tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat
      421 ttgaaattga atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt
      481 tagtaaaaaa ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat
      541 ttttattttg atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca
      601 ttcagtaata aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac
      661 cagttataga aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag
      721 gctgacctgt gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt
      781 atgtatctag atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa
      841 taggctgctt taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag
      901 tgtttttatg aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac
      961 atttgctcgt actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat
     1021 gaaagctgtt gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc
     1081 gttatttcca aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa
     1141 agataaaaca gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct
     1201 tgatttatgt aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta
     1261 ctgtgaaaga ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag
     1321 attagaatgg gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac
     1381 tatgcctgtt gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct
     1441 attagtacct accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa
     1501 aactttcggc ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc
     1561 ttttggagat gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat
     1621 ggataatgca acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga
     1681 agacggtgtt ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc
     1741 atcaaaaaac ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt
     1801 acgaagcgtc tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat
     1861 tctttaaacg aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa
     1921 aggtagtatc gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat
     1981 atgctggaat ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc
     2041 cctctgctga agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta
     2101 aacgaggtac ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt
     2161 tcacgaccac catcgacttc cacagttgaa attgacattt tatttaacac acctattccc
     2221 ccttaactat aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc
     2281 aaatcgccaa tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg
     2341 taaaaattaa accgaagatc tgataaattt tctctatttt ctacaatatt taaaacgctt
     2401 ccttagtcta ttatatatct atttctctta ggagtatgtt tagatgtttg aataatagtt
     2461 gcccaacttt aaaggttatt cttataatat tttacttcat tttccaatag aaaattcaaa
     2521 ggattactgg tttttctaaa atagtttagt ttaaatacat aagtatcctc cgacactgta
     2581 ttagttcata atcatctaaa tacaaaattt tcaaataaaa atatttcaag ctctatcgtt
//
LOCUS       DAGCYL010000223         2621 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.231, whole genome shotgun sequence.
ACCESSION   DAGCYL010000223 DAGCYL010000000
VERSION     DAGCYL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.231"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            35..1486
                     /locus_tag="MB216_002620"
     CDS             35..1486
                     /locus_tag="MB216_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBT4527398.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1835..2506
                     /locus_tag="MB216_002621"
     CDS             1835..2506
                     /locus_tag="MB216_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBT4527399.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
BASE COUNT          984 a          370 c          516 g          751 t
ORIGIN      
        1 acactattaa agagaaaaaa gagaggtaaa tcaaatgaaa aaatctttaa aacttgcatc
       61 agcagttata ttaatgtccc aactctttat agcaacacct ttccatgtgt tggcaagcga
      121 aaattcaaca tacaaaattg caaaagaaag aaccgagacg actgaaatga gtaattccga
      181 tgtaacagca gagagtagtg aacttttgac aactttatca agtgaaagta gtacgacttc
      241 aaatgctgag tcttcaacaa cttcatcaag tgaaagtagt acgacttcaa atgccgagtc
      301 ttcaacaact tcgtcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc
      361 gtcaagtgaa agtagtacga tttcaagtgc cgaatcttca acaacttcaa gtagtacaag
      421 tgaaataact tcttcgaaag tgacaagacc aaaccatata gagtcttcaa aaaaggaagc
      481 gccaaaaacg gaaggacaaa caaaaaattt ttcgacacca cagcaaatag aatcaacaga
      541 atctttccaa tcacaaccaa tactatctgg taatgaagaa attcattttg acaaaaatga
      601 atctgtagaa aattttatta tgaaaattgg tgagtctgcg agaaaaattg gtcagaaata
      661 tgatttatat gcttctgtta tgattgcaca agcgatctta gaatctgcca gtggtcaaag
      721 ccagttagct caggcaccga attacaattt atttgggata aaaggaactt ataatggaaa
      781 ctttgtcatt atggtaacaa acgaagattt gggtaatgga acgctttata cgacccaatc
      841 aaaatttaga gtgtatgaaa actatgagga aagttttgaa gattatgcca aattattaac
      901 taagggaatt agtggaaata aagattttta tgcaggagca ttaaaagcta atagtaaaac
      961 atatcgagaa gcaactaaat tccttacagg acggtatgca acagatacac aatactattt
     1021 gaagctaaat gaattaatta aaacctatga tttaaccaac tatgataaag aagttaaagg
     1081 tccacaaatg agtaagactg gataccaagt gcctattcat aattattcaa taaccagttt
     1141 gtttggaaat cgtggaaatg aatttcaccg aggacttgat atggcagcta cacagggtga
     1201 gccgattcat gcagttaaag ctggtaaggt tgttaaagca gagtttcact actcttgggg
     1261 aaattacgtg gtgatacaac acgaagatgg atcaaccgcg ttatatgctc accagcaaca
     1321 atacctagta aaaactggag ataatgtttt gcaaggacaa ataattggct atgtaggaag
     1381 tactgggaat agtactggcc cacaccttca tttggaaatt tgcaaagata gtagtctttc
     1441 tcagagcatg ttagttgatc cgaaaacctt attattcgga gactaaaaaa ggtaatcaat
     1501 ctgacaaagt aacaagaaaa tggttggatt cttagacctt tgatcaggat attgagactg
     1561 gaattgatat ctcgattacg atgagtttga caaataaaac aagtccatta tcgaatatcg
     1621 agtgtattat tgccgtcttt atgctcatat tttgagcagg ttttactaga tgaaaacaat
     1681 ttatctatct tgaggatttc gtgtacgtat cgttcagtct tgtattataa attagtcata
     1741 aaatcagact aagaaaaatt gaaaatattt ttttctgatt tattaaaagt taattgaaat
     1801 tatatggtac ttctttcttg ggagggtagg tgcagtgaag gaaagatatg gtcaccttac
     1861 tgttgttgag gagattactc ataatggata taaaaaatat cgttacctat gtgactgtgg
     1921 gaatgaaacg attgtatatc gatcaaactt actatcaggt agaaccaaaa gttgtggatg
     1981 tggaaattat aaaaataggc ataaatataa aaatttgaca aataaaactt ttggtaagtt
     2041 agttgcaatc aagccaacta aactgcgaag tgaaggaact gttgtttggg aatgtcaatg
     2101 tctgtgtggg agaaaaaagt tagtatcggc aaagcgactt ctaaaaggtg atgtaaaaag
     2161 ctgtggatgt ttgaaatcaa aaaagcatca cattgaaaat aaaaaattcg gattacttac
     2221 tgcaaagtat cctttggaca ataactgttc atggaaaaca aaatgggttt gtcaatgtga
     2281 gtgtggcagt atgtgtgaga taagttatag taacttattt tttggacata ctcaaagttg
     2341 tggatgttta aaaaggcaag aaacaaggac atgtgttgaa gggacagtaa tagagtgtct
     2401 aacgtcaaaa ttaccgaaaa ataatacaag tggtgttaag ggagtttatc gttctaaaga
     2461 gaaatggatt gcgtatataa ctttaaaaaa aagaggtact atttaggatc gtatgattct
     2521 attaagagtg cagctcaagt tagaaaagat gctgaaaata aattgtttca acctttgatt
     2581 gacaaatatc gaggtaatca ttaattattg tggataataa t
//
LOCUS       DAGCYL010000224         2620 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.232, whole genome shotgun sequence.
ACCESSION   DAGCYL010000224 DAGCYL010000000
VERSION     DAGCYL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.232"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(82..642)
                     /locus_tag="MB216_002622"
                     /pseudo
     CDS             complement(82..642)
                     /locus_tag="MB216_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369693.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            876..2168
                     /locus_tag="MB216_002623"
     CDS             876..2168
                     /locus_tag="MB216_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4527400.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCLISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYIRRLWRPGFKAHLTETDIVDRLLKRSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            2426..>2620
                     /locus_tag="MB216_002624"
     CDS             2426..>2620
                     /locus_tag="MB216_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4527401.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYY"
BASE COUNT          866 a          487 c          469 g          798 t
ORIGIN      
        1 gtagagagtg ggacaatagt cctttctcca aaagaaaaca agtccgattt tgatggttaa
       61 tttcaaaatc ggacttgttt tttcttcttt taaataaggc aagatttctt ctttataaag
      121 ggcataatag aattcgtttg attgcttatc caaagcagtt tcataacgat tcgtttcttt
      181 acgccagaag aaactgaact tgttggcatc tgcttcaatt tttgtaccat caataaatat
      241 tgcttcatta tcaattactt aattggtgat cgattggcgg ctaaataaga caaacgcttc
      301 agctaaaagg tcagcgggtg tttcctgact tcggaatcga ttgatggtcc gatagctgac
      361 ttgttcgtgg ttagccaacc aacacatacg atagctgtca tctaatagaa attcaatttt
      421 ccgaccagaa aagacacgct gcatataagc atatagaata atttttaaca tcattttggg
      481 gtggtaggcc ggtcgtccta gttattctta taattcaaga aagagtgttt gtggcattgc
      541 tacgactaaa gcgttgactg caaaagcgac atctgtttca ggaatatatt gttccatatc
      601 taatggtaaa gttgtttgat tgatgggata atttttaaac ataaagtact cgcttccttt
      661 ctgattgggc ttggtcactt taatcttaca gggatgagtg ctttttgtgt accttaaaat
      721 caaaaaaagt ggggcaataa tcagattttg gctctatact ttttgatgtt ggtggatgac
      781 accagcatct tttttatttt ccaaaacata tagaaaagac accttgaaat cctttagaat
      841 taatttggcg aaccaaccca aaaggaggaa tcaagatgtc ttacactcac cttatcaaag
      901 aaacattaga tattctagac ttatcagtta cttttaacga aaattgtctc acaaaagaaa
      961 aatacaaagg gcaaatctgt catatttatc gtggaaactt gatttataca gcccaagaat
     1021 gcatccactg taaacaccag attgcttcag atattgtgcg ttggggtaca acaaccgtcc
     1081 ggttgttaat gaacgatgtt tctgaatacc gtacctacct cgaattgaaa aagcaacgct
     1141 tcaaatgcaa agcttgtcag cggacctttg tcgcagatac ttctgttgcg aaaaaacatt
     1201 gtctcatcag tgagaaagtc cgctggtctg tcgtgactcg tctgaaaaaa aatacctcaa
     1261 tgacagagat cgccgctcag aaaaatctat cggtctcttc tgtttaccgt attatgaagc
     1321 gattttatcg tccgttgaat ccttttcgga caccgttacc taaggtactt tgttttgatg
     1381 aattcaaatc agttcggggt gtttcaggtg ctatgagttt catcatgatg gatggacaaa
     1441 cacagcgctt attggacatt gtcgagaacc gacaactgcc ttttttaaaa cgttatttta
     1501 gtcatttttc acgagaaatt cgtgaagcgg tcgaatggat cgtgattgat atgtacgctc
     1561 cttatgtatc gctcgttaaa aagctgtttc caaaagcaca gctgattatt gaccggtttc
     1621 acatcgtcca acatattggt cgtacctttc gcaatcatcg aatcaaagaa acgaaccaac
     1681 tattaaaaag caaagaacaa aaacactatc aattaggtaa gcaattaaaa agatactgga
     1741 aacttcttca aaaagatgaa cgcaagcttg attatattcg acgcctttgg cgacctggct
     1801 tcaaagctca tttaacagag accgatattg tggatcgctt actgaaaagg agccctgctt
     1861 tgcgtgtagg ctatcagtta tatcaagact ttctttacgc tgtaaaggaa cgagactacg
     1921 tatcgtttga agaactactt accaataata tcatgcttcc cgaaggctat cagacgacct
     1981 tgagaacgtt ccagaaattt ctgccacaaa tcaaaaatgc tttacaacaa agttattcaa
     2041 atggcccact tgaatgtctg aacaaccata tcaaagtgtt aaaacgaaat gcatatggct
     2101 ttcgtagctt ttataatttc aaattgcgca tcatgattcg tcatggcaac gcattgattt
     2161 tcaactaaaa aagatcaaga ggaactgatc cacttgatct ccaattattc taagatttct
     2221 ctttaccaac atcatttgac aaagagccac ataaaatagc aaataatatc ctaaaacagc
     2281 tatgattttt tccttacaaa gtatacattt ctgattgaat ttatgataaa gtccgtataa
     2341 ttgtgtaaaa gtaaaaaggc catataacag tccttttacg gtacaatgtt tttaacgaca
     2401 aaaacatacc caggaggact tttacatgac ccaagtacat tttacactga aaagcgaaga
     2461 gattcaaagc attattgaat attctgtaaa ggatgacgtt tctaaaaata ttttaacaac
     2521 ggtatttaat caactaatgg aaaatcaacg aacagaatat attcaagcaa aagaatatga
     2581 acgaacagaa aaccgacaaa gtcaacgaaa tggctattat
//
LOCUS       DAGCYL010000225         2538 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.233, whole genome shotgun sequence.
ACCESSION   DAGCYL010000225 DAGCYL010000000
VERSION     DAGCYL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.233"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            238..2391
                     /locus_tag="MB216_002625"
     CDS             238..2391
                     /locus_tag="MB216_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBT4527402.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVLVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          877 a          435 c          552 g          674 t
ORIGIN      
        1 aaataaatga tccatcaaag tgtttcacga cttaagaaaa cataattttc aaaaataagt
       61 ttatttttta atgaaaaaat agttggatat gtaactttaa gtgattgctg tgaaacaaaa
      121 agttgatttt atcttgaaat taggtaaaga atttcttgct ccgcccattt tttctttttt
      181 attaagaagt ttttgggtac aatgaagcat gaatgtaaat aggcggaagg ataggaaatg
      241 atgaaagatt ttatgaaaaa aatattaaat acttcttggt tagaaaaaat aagaaaaggt
      301 gcacaagaac ctaagaacaa aacgtttcgc cggtcccatg ttccttttcg cttaaatttt
      361 ctttttttca ttatatttac gttatttgtt gcgttgattg ctagattggg ttatttgcag
      421 atcgtgaacg gcgaagagat ggaggcgcga gtgaagtcta cttctacgat tacggtccaa
      481 gaaagtactc ctcgcggaat gatctacgac tccacaggaa aagctttagt tacgaataaa
      541 gcgaatcagg ccatcacata tacgagagga acacaagtta cggctaaaga tttgcttgaa
      601 atcgcaacga agctgaactc gctgatcgat gttctggtag atgaaaatct gacagataga
      661 gataaaaaag atttttggct ggctgatccc aaacatttga aagaagccac aaaaagactt
      721 tcggcaaaag aaaagcaatt agggaacagc gaacaatata gtgcgatggt caataaagtc
      781 acagctgatg agattaattt aaacgaggag caattaaaaa ttgctaccat ctttaaacgg
      841 atgaatagtg catatgaatt aaacactgtc tatatcaaaa actctggtgt tactgatcaa
      901 gagttagctg ttgtggcaga acgcgcttct gaacttccag gagtttcgac tggcacagac
      961 tggacacgag aatataatgc agcaagttca ctgaaaagta ttcttggcag tgtgacgact
     1021 gaaaagcaag gacttccagc agatgaagca gagaaatacc tagcaaaagg ttattctcgg
     1081 aatgaccgtg taggacaaag ttatttggaa aaacaatatg aagatgtcct gcaaggaacc
     1141 aaaacccaat atgaagtttc ccttgataat gaaggaaatg tttcgaatca aaaagaaatc
     1201 ttctctgggg aaaaaggttc taatttaatg ttatctatga atgcagaatt ccaatcaaaa
     1261 gtagaagaaa ttttgaaaag gaattatcaa acattgatca acaacggcaa agcacagtat
     1321 tcgcctggtg cttatgcagt ggctatgaat cctcagacag gagaagtgct agcaatgact
     1381 ggtttcagtc atgaacaagg ttccaaagaa atcaccgaga atgctttagg cacgattact
     1441 agcgcctttg atccagggtc tgtagtcaag gctggtactt tgacagcagg ctgggcaagt
     1501 aacgcgatca gcggaaatca agttttgatt gatgagccga ttcgtctgca aggtgcttca
     1561 gaaaaaagct ctgtctttaa tcgaagcgga caagttgctc ttgatgcagt caaagcatcg
     1621 gaattgtctt ctaatacgta tatgatcaaa gtggcgttaa agatgttagg actagactat
     1681 acacctggaa tgggactacc tagtttagat gaagaagcta aagcttatca gcaattgaga
     1741 gactctttca aagagtttgg tcttggtaca acgactggga tcgatttgcc gaatgaatct
     1801 cctgggatct cacgttctgt tgattatatg aagaagttca atgcggataa tggcaaagaa
     1861 tggtatgctc caggtaactt taccgatttg gcatttggac agtttgacac atatacacca
     1921 atccaattag ctcaatatgc ttcaacagtc gctaatggcg ggaaacgagt acagccgcac
     1981 ctagtcaaag cgatctatgg caatgacgaa aatggaaact tgggtgaagt gaagaaagaa
     2041 attggcacaa cagtagaaaa tacggtcaat atttctgcag aaaatatggc gatcctccgt
     2101 gaaggattcc accaagttgt ccatggtacc gatccttaca caacagccaa accattagcc
     2161 agtgcgaaaa tggacctttc cgcaaaaaca gggactgcag aaacggttgc ggaaggtcat
     2221 ccagatattg cgacagtcaa tagtaatatc gtggcatacg gaccaacaga caatccggaa
     2281 attgccatca gtgttgtctt gccgaactta ttagacgaac aagaccacat aaacttgacg
     2341 attgctaaag aaataatgga cgcgtattat gatatgttca tggcaaaata aaaaagaggt
     2401 cgtgaaaaag ccgcttagtt tcgagtatga agaaaaattt tgtgaaaata gcttctcaca
     2461 tttttgctta aatttgactt cataccgaga aactggcttt tgaacaccgt ttactcggca
     2521 aaaaaggttg tgacaaaa
//
LOCUS       DAGCYL010000226         2538 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.234, whole genome shotgun sequence.
ACCESSION   DAGCYL010000226 DAGCYL010000000
VERSION     DAGCYL010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.234"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(38..331)
                     /locus_tag="MB216_002626"
     CDS             complement(38..331)
                     /locus_tag="MB216_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBT4527403.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFNSNQQRKVKKQID"
     gene            complement(467..646)
                     /locus_tag="MB216_002627"
     CDS             complement(467..646)
                     /locus_tag="MB216_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527404.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(643..993)
                     /locus_tag="MB216_002628"
     CDS             complement(643..993)
                     /locus_tag="MB216_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527405.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(986..2311)
                     /locus_tag="MB216_002629"
     CDS             complement(986..2311)
                     /locus_tag="MB216_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBT4527406.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
BASE COUNT          744 a          509 c          385 g          900 t
ORIGIN      
        1 cttatacctt aaatattgct tttcgaaaat agctattcta gtctatctgt ttctttactt
       61 ttcgctgctg atttgaattg aattgatcat attcattttc tagtattaat aacttttcgt
      121 atacatcggt catattcata ctagaagcaa gacttagatc atatacaaat gaagcaggta
      181 atgattttat tctacgttgt cgggttatcc agcttgcaac cgttccttgt gggtaggcat
      241 ataactcaca aaattgttct acagtgattc ctaaattctt gataatgtaa acattgatcg
      301 ggtgcgagta aacaaatgta ttgatagaca tggtgcgtaa gcctactttc ttttattatt
      361 gcgtttgcaa taatagttta acacacgcat tattctagta tctactccta tttgtaaaaa
      421 caaaaaagga ttcatagaga taactctata gaatccgtaa aataccttat atatataacg
      481 ggaaaatata aaacctaaaa ttatgataac ctgctgcagc ttctacatag aaagagaagc
      541 gctcaacacc aacctcattt atcaaacgtt ggccataacc ccaaatctct tctgatagct
      601 gatcaaaggt tatatacttt agctcatatt gctggataat gttcattcta atcgactcca
      661 tttattatcc tggtaaaatt cgatatgacg tatttcgtcg tagtgaattt tgtcttttcc
      721 aacgtagatc cccagctcat cactacctaa gatcttgcca acaatatctt ctggatagta
      781 tccttcagcg tttcgttctt ctttttgaat agatactgat ctatttttta tgactgcagc
      841 gttcaatact tccgcaatct cttcagtcgt catttgttct tttgctaaat tttgactggt
      901 tcgttgttca ttgtcggcat caatttcttc agtatgatca gataaataaa accctgccca
      961 ctttaaacgt ccacgatcat tgtaatcaat catttttaat tcccccattt ccgcccgcat
     1021 gaccaccaac aagtgtgctt ctagtaatgc ttctagcgct ttctaacatt gaagaagcat
     1081 gtacaatcga cgtaaaacca tatttttgcc gaatcttgtc aataatttta tctaaatttt
     1141 cttcgttaat ttgcttttgt ggatcactaa ataaatccag ctgaagcgaa tcggtgtaca
     1201 caagcttgct ataggttatt ccaacgtgcc ttacttcttg tccttcataa tacttatcaa
     1261 acaagaaaag acaataattc tgcaattcct ttgtattatc tgtgataggt attttcatct
     1321 ggtgtgaaaa tcctggtctc gtttctaaga tcgacgtacc aatgtttaaa tgaacacatc
     1381 ctgttttaca attatgctgc ctgatacgaa tggctacttg ttccgccatt tcttttacca
     1441 ccagctcgat ttcatttctt ctagtataat ctcgtggtaa cacttgtgaa ttaccataag
     1501 atttttcctt tgtcggttct ggcgggattg ctatatctgt tcgatcgatt ccgttagcat
     1561 ggaagtacag ctgtaatcca atcacaccta atctattctt aatcgtataa ggattccagt
     1621 tagcaaggtc tctaatgctc aaaattccca tttggttgag cctttttttc attcgagaac
     1681 caattcccca aaagtccgtc atttcgggaa tattccatac tttctcagga acgttttcgt
     1741 acgtccattc cgcaataaat ccttcattat gctttgcttc attgtctaag gctaatttag
     1801 ccaaaagggg attatcgccg acgcctaccg tagcaatcaa acctaactct tctttaatac
     1861 gttcttggat catttgagcg agcttcttcc ttcgctgact tcgtgtttct tcagtcgtaa
     1921 aaagattcag tgattttgtc acttttaaaa tcgattcatc gatcgagtaa atcagtagat
     1981 cttcatcagc cacatatctt ctgaaaatat tatttacctg catatttcgc ttgatatata
     2041 ggttcatacg tggtggaaca acgtgtagtg ttttaggaaa tggttgtggt aagtcacgtg
     2101 gtctactcac atttgtaata ccataccgct tttttgcttc aggagaagaa gctaatatca
     2161 atcctgaacc agtattatca gatcgactca taacaacaag ttctgttgtt aatggatcta
     2221 aatttctttc tatacattcg acacttgcgt aaaagctttt catatcgatt aaaaagtaat
     2281 catttactgg ctcttttgaa taatccatca taaataacac tccattcttc cacattatac
     2341 aaacatatgt tcgatgttgt aaagaagaaa tagctaaata cataaaaaaa gaagcatagg
     2401 tatgctatca ccctgcttct tcttatgatt tgtatgatca atttattcta tcactttact
     2461 aattaatttg aaaatctaat aaaaactctc actctaaaag agtgagagac tgcaaattag
     2521 agtacggact tttttgga
//
LOCUS       DAGCYL010000227         2507 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.235, whole genome shotgun sequence.
ACCESSION   DAGCYL010000227 DAGCYL010000000
VERSION     DAGCYL010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.235"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            84..419
                     /locus_tag="MB216_002630"
     CDS             84..419
                     /locus_tag="MB216_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4527407.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(495..2492)
                     /locus_tag="MB216_002631"
     CDS             complement(495..2492)
                     /locus_tag="MB216_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527408.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
BASE COUNT          796 a          481 c          340 g          890 t
ORIGIN      
        1 ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa cttgttgtac
       61 tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc attagagaaa
      121 agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc tctacacaac
      181 aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc ttaacagctc
      241 tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc gtagattatg
      301 aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt cgaacaattg
      361 actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg aaagattgaa
      421 aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa gattaagagc
      481 tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac tctccgtcca
      541 tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc tgataatatc
      601 aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa aaaactacta
      661 cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag agtgaatcct
      721 tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt atccccaaca
      781 ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag atatgtccct
      841 tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg tttttccttg
      901 ctgagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc gttaaaacat
      961 atcttaaccc attcataaga aaaatattga tatgtaaaat gaattttttc cagaagtgga
     1021 aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc taatcgatag
     1081 acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa cctatttagg
     1141 tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa tcctataaaa
     1201 gtcataagtg atagtaactt attacattta tctatgtcat aagttttacc atagtaagaa
     1261 ctcatttttt tagctaattg ttcccccgac aaaatacaag tcacactttc cttactatca
     1321 tcaaaaaaat taacatcaga aagcaagaca tcatagacac gtcgcatttc ctctagataa
     1381 ttccttattg tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa aagtactttt
     1441 gattcctctt tcaattttcg aaaatgtgta ggatacaagt ctaggttctc taatagccaa
     1501 ttaaatgttt ctacccgact gtctttcttt aaaattagca tcacaactcc aataaaattt
     1561 tcactgatat cacaattact ggaatgacaa taaaaaatgg aatgcccagt tttactggta
     1621 aaaatactag cagagggatt atgatcgttg tgaaacagac atcttaagtt tttacttgac
     1681 ttaagtaatt tattcatatc aatggttaac aatctttcat aaatctcatt aaaagacatg
     1741 tcggaggaat caaacaggca gtctttaaac cactgtttca tttcttcttt gtcgtcattt
     1801 tttatcaact taacaaaatc ggtttcattg actagttcat actctctccc tgaatcactt
     1861 tgacaatttt tagaacatgt catggtaaaa tcaatcggaa gctcagtaat atctaacatg
     1921 ttgtcttcat taattattat cactgcatct gacttatatc ctccaaaaaa cattctattc
     1981 gaagcattta cattgtcatc acacccgggg atgttctttt tcaagtattc gtaaacagca
     2041 accattttac catagtcagt aattgcttct tttaatagaa agacaatctt gtatttttct
     2101 agattttcag agtaccgaat acttttttgt atcgcaaacg cagaattatg aactaaattg
     2161 atagataaaa ccttttctat gcttaagtat ctactatcat ctttactcaa atctactacc
     2221 ttccccttct catctttaac agtgttatcg acatccagaa taattactct ggtttccaca
     2281 aaactctcct tgtcattgcc tttattcttt gtcttgcttg gaataaatga ttgtgatgaa
     2341 tttagtatct ctacaaactc atctcttgta acttctctat agttatctaa acacctatct
     2401 cttacatcag ttttttcttt ttttgtaact ggcttctcta ataatttttc ttgatctaca
     2461 taaattggaa tattaatcat tgatgtttcc atttgtgttt cctcctt
//
LOCUS       DAGCYL010000228         2487 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.236, whole genome shotgun sequence.
ACCESSION   DAGCYL010000228 DAGCYL010000000
VERSION     DAGCYL010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.236"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..618
                     /locus_tag="MB216_002632"
     CDS             <1..618
                     /locus_tag="MB216_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4527409.1"
                     /translation="VADAGYKTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYD
                     AYFDCYLCPNNQPLLFSTITRDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIW
                     ADLMDELEHQRHTALNREIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLT
                     FAAMNLKKLATWLWKTKKPMAFYFKIMQRLTKRSGLITNSFLSTI"
     gene            complement(640..1446)
                     /locus_tag="MB216_002633"
     CDS             complement(640..1446)
                     /locus_tag="MB216_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527410.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(1534..2334)
                     /locus_tag="MB216_002634"
     CDS             complement(1534..2334)
                     /locus_tag="MB216_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527411.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
BASE COUNT          733 a          507 c          325 g          922 t
ORIGIN      
        1 gttgctgatg caggatataa gacgcctaaa tttgttcatt ttctgtcaca cttgaaactc
       61 tacccgattc ttccatatac tgcacctcat ggagtgaaag gaatgtttcg taaaaaagac
      121 tttgtttatg atgcctattt tgactgctat ttatgtccca ataatcaacc gttgttattt
      181 tcaactatca ctcgtgatgg ttatcgaacc tataaatcaa atccaaaatt ctgtgtgagt
      241 tgtccattac ttgaaaattg tactttgagt acaaaacatc aaaaaatagt gactcgacat
      301 atttgggctg atcttatgga tgaacttgaa caccaacgcc atacggcgtt gaatagagaa
      361 atttacaaaa agagaaaaca aaccattgaa cgaatatttg cagatgcaaa agaaaagcat
      421 ggtatgcgtt ggacgaaata tcgaggtctt gaaaaagtcg ccacgcacac gatgcttacg
      481 tttgctgcca tgaatttaaa aaaactagcc acatggctat ggaaaacaaa aaagcctatg
      541 gctttttatt tcaaaattat gcaaagacta acaaaaagga gtggcttaat taccaactcc
      601 tttttgtcta caatctgaat agagcttttc agctctattt tattcaatat cattttgatc
      661 aattaataat cgtacactcc aattaaccga gttttgatca ctcaaatatt tataatcttt
      721 gtcatcaaaa accagttgcc aaactctggt acgaacagtt tcttcgtcca cttcctcatc
      781 tgaagagtat tgcgataatg cttcttcaac tatatacttt acaacttcct caaaatcagt
      841 aggaacttta tcaataacca cttcatagtc ttcttgtttt acgatattgg gatcaatatg
      901 ttcatccaaa catacctcag tgagtttttc tttcacttct gtatctcttt ctgtttttag
      961 gtactctgca tagtcttcct tagaccattg tttttcaaga tcatccgcta tacttttaat
     1021 aaaggattct ttatttaacc aattatctgc ggtatctata ccttcaaact caaaagctgt
     1081 tccttctata cgtgatttga cgaaagcgtc aattttcttg tattgattat tcatttccca
     1141 tgaaacacac tcatcttctt gcgtaatatc ataaattttt tcggaaaaac taactaattc
     1201 attccacgat ttatcctcgg caacttgact tagtttcgat gcaataaaag gaaattttgc
     1261 ttttaagtca tccatattct tgatctcaaa ttcgatattt tcttctccta cagctgaaat
     1321 ccatgctgaa agattgattg caatatcgct aaagtcttct acattttcga catttcctgt
     1381 attatctttt attactgctt ctaaagccat atcggttgtt gcactcatgg tttgatctcc
     1441 tctcataata gagcaaaaag ccggtttaac cgactttttg catggtacgt tttgtttggt
     1501 agttttctgc ttgtgtttat gttgaaataa tccctatagc tcttctttta atagttcttc
     1561 aactttatct cttaactctc cctgaccact ctctaataga tttaaatagc tcatcagaat
     1621 atccgtttca tacgttttta attcagctct atactcactt tctgaatatg atcgtaaata
     1681 cccatcattg ctaaatgcaa ctagatcatc ttgaaagttc cattctccta aatttgtttt
     1741 acttactgct tcccatggac tattactaaa catttcatta aagaaatctt cgtcattttg
     1801 atataccttc aattcaggaa aatagttgct gtcttcattg atttcttgca ccatttttcc
     1861 aagttcttct tcagataatt taccttccaa taattttact ttttctctgt catttgcata
     1921 agccatttga acctctcctt tttctttttc aatttcttca ggtgtttgtt ttggcgtaac
     1981 tttcaaatct attccattgc ctattagacc tattagataa ccatagaacg ttttaaagtc
     2041 acttgtagca agagatttgg catcagggta tgtatcccct ccaattttat ctaaaacaaa
     2101 atcagcaatc gttacccaag gagaatatat cgtacatact acatcccatg tagcatcttc
     2161 aatattcgtc catgtcggaa agtcattttc attcaaatag tcttttttca ttgtttcgat
     2221 cagagttttt ccactttcgt tttggcttag atattcaaca aattctgcac catcaaacct
     2281 gctactaaat agtttagcta atgagtctaa agctgttttt gatactccca ccattactac
     2341 acctcctatt aatattttac gatgacttgt ttttcattca caaataagat tttctacatc
     2401 ttatctattg ctttatagct tttccataca ttatttctaa atacaaccaa taaccctatt
     2461 aaattaagct tttgagttta tcttttc
//
LOCUS       DAGCYL010000229         2428 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.237, whole genome shotgun sequence.
ACCESSION   DAGCYL010000229 DAGCYL010000000
VERSION     DAGCYL010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.237"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            185..703
                     /locus_tag="MB216_002635"
     CDS             185..703
                     /locus_tag="MB216_002635"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBT4527412.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            831..1514
                     /locus_tag="MB216_002636"
     CDS             831..1514
                     /locus_tag="MB216_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBT4527413.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1823..1896
                     /locus_tag="MB216_002637"
     tRNA            1823..1896
                     /locus_tag="MB216_002637"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1857..1859,aa:Arg,seq:tct)
     gene            2055..2414
                     /locus_tag="MB216_002638"
     CDS             2055..2414
                     /locus_tag="MB216_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBT4527414.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          834 a          359 c          481 g          754 t
ORIGIN      
        1 tgttagggta aacgtttttt aaactaacgt caattattta taataaaaaa tgagataaag
       61 atgaggaaat agaattagaa acatataatt attaaaggga aacattttta cagctataat
      121 ttgaaaaaaa tttatcgtta ttatatgatt aagaaggatt gaataaagaa agaagttgaa
      181 aacgatacct gattacaaaa aaataacttt tataagttct ataacgttga ttatattttt
      241 aattcttatt cttctcgtcg tcttggttcc ccgaattttt ggtttgactc cttatattat
      301 tgaggacaaa gaaatgagtc catattatgc acaaggaaat ctgatatatg tacgaccgaa
      361 aaatacaaat gagattttag tcggagatat tattacatat tatgaaaatt caggaaaaaa
      421 aatctcgacg cgtcgcgtaa tagccgttga aaacaaccac gaagattttt atacaaaagc
      481 tgatacaaag acttatattg agcctggagt agtaaaaaag agaaatatta tcggtagtcc
      541 catctttcag attccatata ttggactagt cctttcaaaa acggcatttg catggattca
      601 ggggttattc tttacgattg ctttttgcct aacaggaatc acagcatgga atacctttgt
      661 agagttgaaa aagaagcgga gacgaacaca gaaatttttg taagaatgtt aaaaacataa
      721 atgtattcca gagatccaac gactcttctg aacatgaaac acgagaaaac actcatgcag
      781 cttgtagatt tagtcagttg ttcgtataat gtgtgaagag gaggagtagt atgcgcaatg
      841 aaatgatgca tcgtcctgta gtcaaagaag aactattgga ttttatgcgt acccagcaaa
      901 aaaaattacc gggtgagcta ggcaaactgg aagaagaagc tcatgtagca gaagtaccag
      961 ttattcccca tgaaacagtt gtatttttga aatttttact tggacagctt cagcctgaac
     1021 gaatcttgga gatcggagca gctatcggtt tttcatcaag tgtcatggcc acgacaatcg
     1081 gagaaaacgc tcatgttacg acaattgatc gtttcgatgt catgatcgag aaagctaaaa
     1141 aaaactatga acgtctagga ctgacagata aagtgacttt gcttgaaggt caagcagcag
     1201 atatcttgcc ggagttgtct ggtccgtatg actttatttt tatggatagt gcaaaatcaa
     1261 aatatattga atttttacct gaatgcttac gtttgttaag aaaaggtggc gttttgatgg
     1321 tagatgacat cttccaagga ggaacgatct tgctcccaga tgaagagatt ccccgtggca
     1381 aacgagctat ccatcgtaaa ttaaatgaat ttcttcgtgt tgtgatggat catcccgatt
     1441 tgacatcgac aatcttgcca ttaggtgatg gtgtgatctt aatgacaaag gaatcagaaa
     1501 caatcgagtt gtgatacatg attatggcag cctgtagata atcgattgaa tcgagggctc
     1561 aattcgaact tgatgtaaaa gtttctatat ttgtaagtca attaaaggat acaggaaatc
     1621 taaataaaca ggagtagcat gaagtcaatg tagctcctgt tttcaattga taaccttaga
     1681 acagctgaag gagaatgtct cgttttaaaa gattgattta gtagttgggc caaaataatt
     1741 cattatttgc taattaaata actatttcgt attgacagaa gcaatttaat tatctataat
     1801 tattgatgtt ccgctgattc ttgtcatagt agctcagctg gatagagcat ccgccttcta
     1861 agcggacggt cgggggttcg aatccctcct gtgacgctaa aaaaccgtta gaaactgagg
     1921 tttctaacgg tttttctttg ttttttgtgc caatattgtt tgttttcaaa ggcaaataaa
     1981 ataaaaaaac atccttatcg agtacaatta taaggagcaa aaaaataaaa ctcatcagaa
     2041 aagaggaaga agatatgacg atactatcta gaattttagt gactctagtt gcaattgaat
     2101 ttttttatat catgtatatc gaaacggtga gaactgattc tgacacgaca agtagggttt
     2161 ttaaaatgag taaagaagag ttaagcagaa aatctgttca aacattgttt aagaaccaag
     2221 gagtgtacaa tggactatta ggagtgggac tactttatgg agcttatctt tcttccgcat
     2281 ctaaagaaat cacaagtatg ctattaatat ctattttttt tgttgcatta tatggcagtt
     2341 tgactagcga tcgttggatt ttactcaaac aaggtgggct agctctgttg gcattgcttt
     2401 ctattttctt ataaatatag aaaaaata
//
LOCUS       DAGCYL010000230         2400 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.238, whole genome shotgun sequence.
ACCESSION   DAGCYL010000230 DAGCYL010000000
VERSION     DAGCYL010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.238"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            207..2069
                     /locus_tag="MB216_002639"
     CDS             207..2069
                     /locus_tag="MB216_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4527415.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="MB216_002640"
     CDS             2069..>2400
                     /locus_tag="MB216_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4527416.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAGCYL010000231         2388 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.239, whole genome shotgun sequence.
ACCESSION   DAGCYL010000231 DAGCYL010000000
VERSION     DAGCYL010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.239"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(366..1658)
                     /locus_tag="MB216_002641"
     CDS             complement(366..1658)
                     /locus_tag="MB216_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBT4527417.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1932..2192)
                     /locus_tag="MB216_002642"
     CDS             complement(1932..2192)
                     /locus_tag="MB216_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527418.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          805 a          445 c          364 g          774 t
ORIGIN      
        1 gtatgggcta ctctgtgata cttattagcc attaaactat tttacccaca tagcatatat
       61 tttgaaatcg ttcattccgt aaattcaact aaatatgcct aaaaaaataa aaaatctaag
      121 atagaataag atttattcag tcttagatgg atttatagca actttttcgt aacaacttta
      181 aaaaatacga aaagaaagag ctgttttact ctgactacgt tgctaaattg tcgtttaatc
      241 attcatttaa accaaatctt tttttataga attcgtttta atcctcctga atccaaaaac
      301 tatgaaaacc gtaattaaat agttaagagt ttttaaccac cataattctc tcggctattt
      361 tgaaatcaga taaattttga tcggacacat agttgaataa acctaccgtt gttacggaag
      421 gtaaatcgca tacttttcat tctagaggag aaggtctcgt tgatcattaa ctgattaagc
      481 accatagccc agttttgaat tcgaccacct gaccacttcg cgtgtaattc tttcgtgcgt
      541 aaataaagta gttttaggag tgcattctca ttagagaacg ctcctttttt tgtgactttt
      601 ctgaagctgg agtggacact ttcaaccgca ttggtagtgt acataatttt tcgaatggca
      661 ctaccataat caaatagttg ttcaacatgt gcaaagttcc gtttccagac atctacagca
      721 ccagaataat gagaccaccg attttgaaaa ctgtcaaaag cagcatgtgc agcgtttaga
      781 gaagaagcac cgtagaactt tttcatatct cggcagactt ccttatagtc cttacttgga
      841 atatagcgca atgcatttcg aacaagatga acaatacagc gctgaacaat taccgatgga
      901 aagatcgctt ttgcgccttc ttcgaggcca gaaacaccgt ccatcgaaat gaaaaagaca
      961 tcttcgacac cacgtgcttt cagttcatca aatacttgca tccagcgatt tttagattct
     1021 gtttgattta accataatcc taaaatctcc ttatttcctt tgagatcata gccaagaatg
     1081 gtgtatacag catattcttt ggcttcataa ttttctcgta aagtaacata catacaatca
     1141 acgaatagaa aggcataaca ctttgctagg gggcgggctt gccattcttc caattcagga
     1201 aggacagcgt cagtgatatc tgaaatcatt tcatgggaaa tatcaaagcc ataaatagct
     1261 tcgacggttg cggcaatatc tcgttgactc attcctcgtg catacatgga aagaaccttc
     1321 ccttcgatgt cggagacatc tcgttttctc ttaggaatta actctggttc aaaggaagct
     1381 tcccggtctc taggaacatc aatagctact tcaccaaaac tggttttaag cgttttagtt
     1441 ccatagccat ttcgacggtt atcgtgttcc ttaggctctt tagaatgggc atcataacct
     1501 aaatgattat tcaattctcc ttgaagcatt ttttcaaaaa ggggcccaaa cacatctttc
     1561 aaagcatctt gcatgtcatc gacagattca ggttgatagg cattcagaat ggattcagct
     1621 aacttttcgg catcaggatt tcttttcttt ctagccatcg tgtaatcacc tcttgatctt
     1681 attgtagaaa aagaaaaacc gcgaagcaac cacctgccta ggattaatgg ttacttacac
     1741 ggtttatatt acactctcat cattaaaaaa gtatctttcg atattactaa acaatgaaat
     1801 tatctatttg aaatttaaat ctgataatat attgagtgtt ccaatgaggg ctttgatcct
     1861 attatccata tctttttcag ttttaatatt gatacacatt ataagaaaaa gaaacttttg
     1921 gttaatttat ttcatcccag tgcaaaatat aaaaaagtaa aatagaccat aggcaataga
     1981 ttaacaatta atataataca agcaaatatt ctattcattg tatttctgct ttcaaaaagt
     2041 tctaatattg aaagtataat tccacaaatg cctataataa gtatgggaat aagattagaa
     2101 aataacaccc cgagccaatc tgtgaaaatt actatattta ctaatatccc tattaacata
     2161 cctatcatat ttattaaatc aatttttttc attagatatt tctcctttat taaatctgaa
     2221 gtacttatat taaaataatt ctaaactatt tacctcaaaa agtgcaataa attatttctg
     2281 aacaataaaa tgatagtcaa aacaaaaaaa tattgttaat cgttcagagc catccgagag
     2341 tgtaaaatat tttgtgtaaa tgaaaaaatc catacaaaaa aggaaggc
//
LOCUS       DAGCYL010000232         2278 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.240, whole genome shotgun sequence.
ACCESSION   DAGCYL010000232 DAGCYL010000000
VERSION     DAGCYL010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.240"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            163..>2278
                     /gene="acm"
                     /locus_tag="MB216_002643"
     CDS             163..>2278
                     /gene="acm"
                     /locus_tag="MB216_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBT4527419.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          893 a          347 c          445 g          593 t
ORIGIN      
        1 aaacagtttt ttggcatatt tattttttat aaatatttgt aaaataacta tcaatttttt
       61 agttaaaaga ttggaaatga gtgggggagg aattgaagaa aggatttttg agagatgata
      121 tagtaggttt tatttttagt taaaataggg gagatagaaa aaatgaaaaa gtgtagaaat
      181 attattttat caatgttatt tattattact aatataacat cactgattcc tgttcatgtc
      241 tatgcggatg caggcagaga tatcagcagt aatgtcactt cgttgaccgt ggacccaacg
      301 aatattacag atggaggaaa cataaaagta aaattctcgt ttgacgaaaa aaaacaaaat
      361 atccaaccag gtgattatct ttggatcaat tggccaagtg aaggaaatat tcgcggtgaa
      421 ggattccaaa aagaaatccc attaatgatt gaaaataaaa atgttggaac tttaacggtc
      481 agaaaagatt ctgcgcaggt tgtgtttaat gaaaatatta aaaacctaga ctcagtagaa
      541 ggttgggggc aatttgagat acaggccaga aacgtaaccg ataccggtga agaaaatacg
      601 ggaccattca ccgtgacgag cggtgataaa acagctactg taaatgtgac aaagccagct
      661 tctggttctt cttctagcgt tttttattat aaaacaggag acatgctacc tgaagatact
      721 aagcacattc gatggttttt gaatattaac aataacggca cttatgttga acaaccagtt
      781 aaaatcagtg atgaaatcca aagtggacaa agattagatc caagtacgtt tgaaattaat
      841 caaatacact taggtgaaca gaaggtttat agaggagaag agggaatcca acaattttta
      901 caagatttcc ccagcgctac atttaatttt tcagtaactg ataattacat tgaaataact
      961 ataccaaaaa actttgtcaa tcttaggaaa ataatggtga gttataagac gatcatagaa
     1021 aacccagaac aaataaattt tgagaatcat tcggaagctt ggtttaaaga atttaataaa
     1081 cctgctgttg atggagaaag tttcaatcat acagttaaaa atatttcagc ttctggtgga
     1141 gtcaacggta cagttagagg cgaattgaag attttcaaat atatcaatga cacagagatt
     1201 ggtatcccaa atgtgacttt cgaattaaga cgagcagatg aacagcctat tcaaggacag
     1261 tctagtatcc tgttgacttc gaatgaacag ggagaagcta gtatcaaagg cctgcaagta
     1321 ggagactatg ttgtaaaaga aaaagaagca cccaattgga ttgattttga tccactaagt
     1381 tcaaatgaac tcaaattctc cataaacgaa aatgatacag agggagtcag tttacctatt
     1441 tataacaaga aaaaggttac gaacatcaca gcaactaaaa tttggaacgg tggaaccact
     1501 ccaagaccga gtatttattt taaattattc agagcaagta caaacaattg ggaaccagtt
     1561 cctgatgcag aaacaaaacg attagacaat ggaataactt cagtgacttg ggaagatatc
     1621 caacagtatg atgatagtgg aaacgaatat acgtttaaag tgcaagaagt agatcaaaac
     1681 ggaaatgatt atgtcccttc aggttatcaa aaaattgaaa acgggctagt cgttacaaat
     1741 gagaataaag aagtcatttc agtctcagga caaaaaacat gggaagacaa tgagaaccaa
     1801 gacggcaaac gaccgacgac gatcacagta aatctattag ccgacggtca accgatccaa
     1861 cacaaagaag taagtgaaaa agatgattgg cgctatcgtt tcacaaatct gccaaaatac
     1921 agagacgggc aagaaatcat ctatactgta acggaagaca acgtgcccga atacagtacg
     1981 acgattgacg ggtataacat caaaaacagt tatacaccgg aaaaaacaaa tatatctata
     2041 ccaggtagcc atataacacc gtggaaaaat tttccagaga agacagagag agagaaaatt
     2101 agaaatctat tctctcaaaa attgcaaata tttgctcata caggagaatc cagcgaacaa
     2161 agaagcaatt atattagtaa gagagctgta cctaaacaga taacaaataa atcaaaaagc
     2221 attcttccta aaacaagtag taaaaaatct agttttgctg taatcattgg tttattga
//
LOCUS       DAGCYL010000233         2272 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.241, whole genome shotgun sequence.
ACCESSION   DAGCYL010000233 DAGCYL010000000
VERSION     DAGCYL010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.241"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            4..>2272
                     /gene="esp"
                     /locus_tag="MB216_002644"
     CDS             4..>2272
                     /gene="esp"
                     /locus_tag="MB216_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBT4527420.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT          821 a          349 c          465 g          637 t
ORIGIN      
        1 ataatggtta gcaagaataa taagagagta tttctagaaa aaacaaagaa acgagtattg
       61 aagtacagta tcaaaaaatt aagtgttggg gttgcatctg ttttggttgg tgttggtctt
      121 gtcttgggaa caactgaact agttcaagca caagatgaaa tttctccaag caccccactt
      181 gaaacagcga taagctccgt acaagtaggt gacaaagtag cttcggggaa tacttttcag
      241 gagaatcctg gttatacgaa aaattataac ttttcggatt tacaatttag tcctcaagaa
      301 ctaactggag acacactaaa aggcaataca attggatttg aagtttacgg aaaacataat
      361 attgcagcat caactaaaaa ctgggaaatc cgtcttcaat tagatgaacg attagctaaa
      421 tatgtagaaa aaattcaggt cgatccaaag aaaggaattg gatcttctag acggaccttt
      481 gtaagaatta atgattcgct gggtagacct acaaatattt ggaaggtcaa ttatattcgt
      541 gcaagtgatg gattatttgc tggggctgaa acaactgata cacaaactgc tcctaatggg
      601 gtaattacgt ttgaaaagag cttagatgaa atttttaaag agataggcat agacaatctc
      661 aagaccgatc gtttaatgta tcgaatctac ttggtaagtc atcaagacga tgataaaatt
      721 gtaccaggta ttgatagtac gggctacttt ttaacagatt cagatgattt ctataatagc
      781 ttagatgtat ctgaaaataa tcctgaccag tttaaacatg gatcagtaag tgcaaaatat
      841 gaggaaccta atactcaaac aaaagatggt tcaggatcca caggtgctaa tggagctata
      901 atcttagatc ataagttaac gaaaaattat aatttctcat attcagcttc cgcaaagggc
      961 acgccttggt atgctaatta caaaattgat gaacgattag ttccatatgt agcgggtata
     1021 cagatgcaca tggttcaggc tgacaaagta acatatgatg tttcttttga atcagggaaa
     1081 aaagtggctg atttagcaat tgaacggcgc aaagatcatg aaaattatgg tgtaggttca
     1141 attactgata atgatcttac caaacttatt gattttgcta atgcaagtcc acgtccagtc
     1201 gttattcgat atgttttaca attgactaag ccattagatg aaatcttaga agatatgaaa
     1261 gcaacagcac aagttgaaga aaataaacca tttggtgaag atttcatctt tgattcttgg
     1321 ttgtcggata cgaataaaaa attaatccag aacacttatg gaacaggtta ttattatttg
     1381 caagatattg atggtgatgg aaaccctgac gataaagaag agagcggaga cacgaatcca
     1441 tatatcggga aacctgaatt agaagaagta tatgatgttg acacaacagt taaggggaaa
     1501 gtattcatcc acgagttagc gggaacaggt cacaaagccc aacttgttga taaagaaggt
     1561 actgtattag cagaaaaaac tatcgctcca aatgaaaaag atggggctcc aatttcagat
     1621 actgtagaat ttgaatttac gggtgtagat tcaagtaaat taatcgcgaa agatgaatta
     1681 aaaatccaaa tcgtttctcc aggttttgat aaaccagaag aaggttcaac cgttattaag
     1741 gaatcaccaa aagcggttga taaacaaacc gtggtagttg gatttaaacc agatgctaaa
     1801 gaatcaattc ggaataataa aaacttacct gaagatgcag agtattcatg gaaaacagag
     1861 cctgatactt ctaacgttac tgatagtacg aaaggtattg taactgttaa gatcggaaat
     1921 cgaactttcg acgtggatgt agagtttgct gtaaaagctt ctcaagctat ggaaaatgat
     1981 gcaacatacg tacctataac aacaactcca gaaacgacag ttcaaagtgg aaaacctaca
     2041 tttgataaac cagatgttcc tttagctaaa gatgcctttt caattttaga tgtctataat
     2101 aaggacttcg gtaatgcaag tgttgatgca aatactggta ttgttacatt cactccagct
     2161 aaaggtgtag gagaatcgga gccgattact ggaacaattc ctattaaaat tgtttaccaa
     2221 gatggttctg taggcacgac cgatttagca gtaactgtaa gtaaagatat tt
//
LOCUS       DAGCYL010000234         2233 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.242, whole genome shotgun sequence.
ACCESSION   DAGCYL010000234 DAGCYL010000000
VERSION     DAGCYL010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.242"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            118..564
                     /locus_tag="MB216_002645"
     CDS             118..564
                     /locus_tag="MB216_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBT4527421.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            837..2189
                     /locus_tag="MB216_002646"
     CDS             837..2189
                     /locus_tag="MB216_002646"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBT4527422.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT          779 a          367 c          448 g          639 t
ORIGIN      
        1 tgttttacgt cttttttcat taaaatacaa taaaattgaa ttttcttttc atgttaagtg
       61 gattttaaaa tgtaaaaata gtatgataag acgagaataa caacgtgggg tgtttacatg
      121 gaaaataagt tagtcgaaga ggttatcgaa aagttaaaaa aagagaacat tcgaatcaca
      181 cctcaacggt acgcggtttt agaatatttg attgaacatc attcccatcc aacggctgat
      241 gagatttata aagcacttga acatcggttt ccaaatatga gtgttgcgac tgtttacaat
      301 aaccttcgct tatttacttc gattggattt gttcaagaaa tgaagtacgg cgatgcttct
      361 agccgatttg atttcagttc aaagcgtcat tatcatgtaa tttgtgaaaa ctgcggtaag
      421 attgttgatt ttcattaccc agggcttgaa gatatagaaa tggctgcaag cagactgact
      481 gattttgaga ttaatgaaca tcggttggaa ttgtatggac tctgtccgga ttgtaaagag
      541 tcagcaaaaa aggcgcaaat gtaaaaacag tttctacaag tgttatacct aaaaaaagct
      601 agctgtgtaa aaaaaacgct agctttttta tttttttgtc ttttattcac aatcttagat
      661 ataaaagtga atattattaa aataagaatc ttgtagatat tgggttctta tgcttgaaaa
      721 ggaatgaaac tacaaacaaa cctttgtgaa ttcacaagca ttaaactgtt gtatccgttt
      781 tatttctgtt atagtagttt tgtcgaagga caaaatgtta atcggaggga ccataaatga
      841 aagtagtagt aattggctgt actcatgcag gtactgcagc tgtaaaaagt attttaaaaa
      901 atcatccaga agcagaagtg acagtatatg aaagaaatga caatatttct ttcttatctt
      961 gcggtatcgc actttatgtc ggcggagtag taaaggaccc agcaggcttg ttctactcaa
     1021 atcctgaaga attatcttca ttaggagcaa cagttaaaat ggaacacgat gtaacagacg
     1081 tcgatacaga aaataaaaca gtaacggcaa aagatctgaa aacaggagaa acagaaacgg
     1141 tttcttatga taaattagtg atgacaactg gttcatggcc tattatccca ccgattagtg
     1201 gaatcgatgc gaaaaatgta ctcctatgca aaaactacaa tcaagcaaat gaaattattg
     1261 cacaagcgaa agaagcaaaa cgtgtagtaa ttgttggtgg tggatatatc ggtattgaat
     1321 tagtagaagc ttttgtcgaa tctggaaaac aagtgacatt gattgatgga ttagatcgca
     1381 tcttgaataa atatctagat aaaccattca cagatatctt agaaaaagaa ttgacagacc
     1441 gcggtgttac tcttgcgcta ggcgaaaatg ttactgaatt tatcacagat gaagaaggaa
     1501 aagtgaaaca agtagctaca ccaacagata catttgatac agatatggtc attttatgtg
     1561 tgggcttccg tccaaataca aaactgatcg aaggaaaagt agatacactg ccaaacgggg
     1621 caatcaaagt gaatgaatac atgcagacaa gtaatccaga catttttgca gctggagact
     1681 ctgcagtagt caactacaat ccaagtggca cgcaaaacta tattcctttg gcaacaaatg
     1741 ccgttcgtca aggtttgctt gtgggaaata acttgacaga tcataaaatg gcgtatcgtg
     1801 gtactcaagg aacttcagga ctttacctat ttggctggac gatcggttcg acaggtgtga
     1861 ccaaagaaag cgcaactttg aacgatctag aagtccaagc gactgtattt gaagataatt
     1921 atcgtccaga gttcatgcca actactgaaa aagtaatgat ggagctagtc tatgaaaaag
     1981 gaacgaatcg tatcgtaggt gctcagttca tgtccaaata cgacattaca caatcagcaa
     2041 ataccatgtc tttagctgtt caaaataaaa tgactgtaga agatctcgca ctttcagatt
     2101 tcttcttcca accacatttt gaccgcccat ggaattactt gaacctactt gcacaagcag
     2161 cgctaggcga aatcgaaact gcacaataaa gaaaaagggt tgtgacacaa ttttgtcacg
     2221 accctctgtc ttt
//
LOCUS       DAGCYL010000235         2185 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.243, whole genome shotgun sequence.
ACCESSION   DAGCYL010000235 DAGCYL010000000
VERSION     DAGCYL010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.243"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            359..814
                     /locus_tag="MB216_002647"
     CDS             359..814
                     /locus_tag="MB216_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527423.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(976..2148)
                     /locus_tag="MB216_002648"
     CDS             complement(976..2148)
                     /locus_tag="MB216_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4527424.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT          701 a          436 c          392 g          656 t
ORIGIN      
        1 aaaaatcccg tgtatcaaaa cgggattaga ttaagtttcc tcaatcacta ttcataacac
       61 aattattata acaagtaagt aaacaattga aaaggacatt tattgaaaac ggttgttttt
      121 tacttaagga ggaaatttag tgaaaaaatt agttgcattg tttttggtcg caggaagttt
      181 gttgagtggg tgtggtgcta ctacgtctac tactcaaagc acaagttcag ctcaggcttc
      241 cagcagcagt accacgatct ccgaaagtac ggcagattca tcgaaagaac aagaacaaat
      301 tgaaaagaaa aaagaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaaagt
      361 gacagaagcg gaatctgctt tgtcacaagc tgaagcaaat cctacaaaag aaaactatgc
      421 aactgcttca gcagctatcc aagcgctacc tgaaaacaaa caagagttga caagccgtct
      481 tgctgcaatt gattccacaa tcaaagcaaa agaagcagaa gaagaacgga aaaagcaaga
      541 agaaattgca cgacaacaag cagaacaagc gcaacaagaa gcaatcgcac agcaacaagc
      601 agaacaggcg cgacagcaag cagcagctga acaggcctct cagcaggcac aagccgcagc
      661 tcctgagcaa aataatgaac aaactgtcta tgtcacaccg accggttcaa aataccatac
      721 acataaatgt ggaaatggta cgtatacccc tgccacttta agcgaagctc aagctcgtgg
      781 tttaacccct tgctccaaat gttatggaaa ctaattcaaa agttatacta taataataaa
      841 attcttctga aacataaaaa aatagaagct ggcgttccag atagtaaacc aaaaatagca
      901 gtttacacgt ccatttcgtc agcttctctc ttttttcatt tagtctgttc cctctgcttt
      961 tttggacatc ttcattcatt ctttctcaat caaatagtac acctcttttt cttttcgcag
     1021 tttatatctt ccttttttca atttcattga aaactgtttc gaatgtttat gttttacatg
     1081 gattttaagc aggagctcct catgttgcaa gcgatagttc ctgcctttta aatcagaaaa
     1141 tctcactagt ccgtattcat cagaagcttt cacaaatgga actttttctc tagtaatata
     1201 gtgtttgccg ttgagcgttt ttacagaaaa gaccattcct tcgattggtc gtccgtttgt
     1261 gttctttaca taaaaagata aaggatagtt tcgcttagcg atggccaaag aatctaaata
     1321 agcgatgagt agccaaacta agccgattga tccaagtagt ataccgataa accaaataaa
     1381 aaatttccat tttttccatt gatcaattcc ttggatcgct ttttttgcct cttttgcttg
     1441 ataaggaata cgatgccccg tcacaagcag tcgatgtgta ttgaccatat aaggagtaca
     1501 ggtaagcaaa gtcacgagat ctctgccttt ttggataccc aaagaatcta cttcatcagg
     1561 taagaccact tggatttttt ctacttgata cgccaatgtc tttccattga tctggatgaa
     1621 aaactggtct ccttttttca acttaggcaa atctgtaaat aattttgctt ctgggagtcc
     1681 acgatgccca gaaagaacgg catgggtgtt tttcccgcca gtcggataag aactttcttc
     1741 caataatgac gttccttttt gcaaaaatat ttccgttgtc tgatcgaata taggtaagct
     1801 gacattgatt tttgggattt cgatgactcc aagcatatgt tccataaaaa aggcattact
     1861 aggtaaatct tttaatgctt ttgcatcaac tgcttggttg aaactgccta tccctggtga
     1921 tacgttttgc tctgccaact gttggttttt ctttgtcatt ttttcctgct gtttttttat
     1981 ttcctctgaa ttcttattcg atgcttgctt ttgataatgg atgatcattt gctgggcaag
     2041 aaaatcattc agtgaatctt ctacaaaagg ataagaaaag atcccgatcc caacgactaa
     2101 aatgattgcc atgaagatgt ccagtattct tattctgctt ttattcatca tgtccaccta
     2161 ttttctaata tgtatatgaa gaggt
//
LOCUS       DAGCYL010000236         2178 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.244, whole genome shotgun sequence.
ACCESSION   DAGCYL010000236 DAGCYL010000000
VERSION     DAGCYL010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.244"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(592..957)
                     /locus_tag="MB216_002649"
     CDS             complement(592..957)
                     /locus_tag="MB216_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527425.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1033..1278)
                     /locus_tag="MB216_002650"
     CDS             complement(1033..1278)
                     /locus_tag="MB216_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4527426.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1348..1602)
                     /locus_tag="MB216_002651"
     CDS             complement(1348..1602)
                     /locus_tag="MB216_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527427.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1766..2089)
                     /locus_tag="MB216_002652"
     CDS             complement(1766..2089)
                     /locus_tag="MB216_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527428.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          824 a          365 c          378 g          611 t
ORIGIN      
        1 agctttttta aagaatattt agaaattatt aattttcgtc tgaaaacaaa aaaagaaagc
       61 gttattattt tagtctattt tcaggggggg aaccttatga taaaaaaact atttttacta
      121 tcaatattaa cgctagcaat ggcaggttgt tctgactctg gcttagcaac tgaactgatc
      181 acaaaatcaa ctgaagccac gacagaggag ttaaaatgtg gaaacgatca cagtgactac
      241 agaggcttaa atcgacgatg catgggaata ttaggcagaa gcaattgtaa gcatcgatgt
      301 agtaatcgaa gaaggtgtag aagaagcaac agaaaaatca aaagaaagat caaaagaagc
      361 aatctgtgct gtgcatcgta tacagacgtc gaagatgcgg tacttttaaa ccacgtcagg
      421 agatgttcat ttatgtctag ggaagttggc gatgaaccaa aactagagta cgcttttagt
      481 tttgcacgtg aggatgaaga taaccctaaa acttacgaca ttttagcttg atctaagatg
      541 gttaaagctt tttaatgaag taagaggaga tagaaggctt ttctagttat attaggtaag
      601 ataaattcct ttttgctcca aatttgctaa acattctgct agataaactt catcgtgttc
      661 cggataaatc ggcacgttgc gctctacagt aggtaaatca aaaaatgctt cgcatgattt
      721 gccacgatac ataggatttt tccactctgg tgctgggctg taaccacgtt tttccatttc
      781 atccatgaca agctgatgat attggaataa tctataagga gaatagtgaa aaacataatc
      841 cacagtagca tgtttctttc cccagccatt tcctcttaat gcacagcatt cccgatgttg
      901 tcctaaaagc tgttgacgag gaagctttga aatcaatttt tcatgccata atcgcataga
      961 aatgtctcct ttatatatag attgttaacc ttagacgtta aatgaaaaaa ccagcaccaa
     1021 ggctggaaaa aatcacttca gtttgcttgc tgtttctgag cgaccaatta ggtaatcaac
     1081 ggaaatatcg taaaagtcag cgatatagat taattcaatg atgcttggaa tttcttctcc
     1141 ttcttcgtac ttacgatatt tgaataaagg aatacttaac gcgctagaaa cagctacttg
     1201 agataaagat cggccagttc ttaagaaatt cagacgacaa cgtaaagtat ccagtaaaat
     1261 agcttcttcg atactcattt aatcaccttt cattatagta gaatagttat aatgactata
     1321 gaaagctaca catgcaatca atagtactca atgtttttta tttaaacgta tcaaataaag
     1381 aatgacgatc aataaagaca aaaccagtcc tgataattct tctataaaaa cactatcggg
     1441 ttcaaagtag ttgtaattaa aaactttcca cataaagtag atagccggaa gagtcaaaat
     1501 agctaaataa aaccgttctt tgttccaatt cataagataa acaatacttg cgaccagtgc
     1561 gaccgcaggc acccaaaaca aaactctcac caacgtaatc atgtaacgga ctcctcaacg
     1621 attcatataa taaaagataa catgttattg catacatcac aaatgaaatg ctaggaaaat
     1681 aaaagaagaa tagatcaatg aaaaatgaaa aagcagaaca gttttctgct gataagcagg
     1741 aactgttccg ctgaatgtgt cttatctatc gagaaaaaat caatggattg atatctaagt
     1801 aattcaatac atataaaaag aggacgatcg cattcactaa aaaggaaaga atgatgatcc
     1861 catagcgaat ggaaacatat ttaacaaata cttttgtttg aatacgggca tatctttttc
     1921 ctttatagct gagcatcatg acaagaagca aaagggtaat cacaccttga aaaatcattc
     1981 caataaacag aattcctgag gtttgataat ttaattggag aaaataagct gctgccgcaa
     2041 ctaaatcgaa taaaatcgca ataaatggca aaataatcac tcctaacatt gagaataaat
     2101 aaaagaataa cattttttct aaaataagca aataaaaatt aaagtaacaa aatagttatg
     2161 acacaagtaa aagaaata
//
LOCUS       DAGCYL010000237         2170 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.245, whole genome shotgun sequence.
ACCESSION   DAGCYL010000237 DAGCYL010000000
VERSION     DAGCYL010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.245"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..914)
                     /locus_tag="MB216_002653"
     CDS             complement(<1..914)
                     /locus_tag="MB216_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBT4527429.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNHTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="MB216_002654"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="MB216_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBT4527430.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..>2170
                     /locus_tag="MB216_002655"
     CDS             2006..>2170
                     /locus_tag="MB216_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527431.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFH"
BASE COUNT          737 a          383 c          415 g          635 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatggttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatatgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caattcttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa accagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat
//
LOCUS       DAGCYL010000238         2129 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.246, whole genome shotgun sequence.
ACCESSION   DAGCYL010000238 DAGCYL010000000
VERSION     DAGCYL010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.246"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            114..1061
                     /locus_tag="MB216_002656"
     CDS             114..1061
                     /locus_tag="MB216_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4527432.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            1191..1931
                     /locus_tag="MB216_002657"
     CDS             1191..1931
                     /locus_tag="MB216_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527433.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          747 a          348 c          443 g          591 t
ORIGIN      
        1 actcatgacg atagaggatt ttccgttttt tattttgggc tataataaac tctgttagga
       61 aaatttggta taattgatag taagaatgac aacatgttag aaaggaagag agcatgccca
      121 ctttagcaga tgttgcaaaa cgtgccaatg tttcaaaaat gacggtctct cgagtcatca
      181 atcatccgga acaggtgact gacgaattga aagagttagt tttctcagca atggctgaac
      241 tggattaccg tccaaatatt gctgcaaaag cacttgtcac gaatcggtcg cagatcatca
      301 aattatttat actggaagaa atcgacacaa ctgagcccta ttacatgaat ttgctgatgg
      361 gaatcgctaa acggatcggt aaagcgcatt attctctcca attagttaca aatgatgcct
      421 tcgacgtcgg ttcttgtgat ggatacatca tcacaggtgt cagaaaaaaa gacttcgatt
      481 ggatcgaccg tttggaaaaa ccagttgtct tgtttgggga aaattctgaa ggatatgatt
      541 atgtagacag cgataatgaa tatggtacag caaaagcaac gcaatatgcc ttagaaagag
      601 attatcaaac gattatttat atcggtatcg aagtggacga gccttttgaa atttcaaggg
      661 aaactggtta tttatctgtg atgaaggaag ttgatcttcc tagtaaaatc ttgcggtttc
      721 caaatcattc gacagaagta gaaagatata tgacagaaaa ctgggcagat tttacagata
      781 aaacttgttt tgtatgtagt tcagatcggc tagcactagg agcagtaaga gggattataa
      841 atgctggagg acacttgccc gaagaatttg gtgtgattgg atttgatggg gtatttttag
      901 accaaatcag ttcacctaaa ttgacaacga tcaagcaaga tattatacgt atgggtgaag
      961 tatgtggaga aatgttgtta aaaaaaatag aagaaaaagg ggcgagtcaa ggttatcgtc
     1021 gtttttttcc tgaactagtt atcagagaga caacgaatta agtcttatta ctatatatat
     1081 atttatgaaa ttcatcgcta ttttcttaaa ataatcagcg cattgctact gaaaacaatg
     1141 aatctgtgct attattgaga aaagttccat ttttgagaag gatggtttga atgaaagaaa
     1201 tcagacgaaa acatttccgt tttcctgcgt acgatgatga gttaggcgta aaattaacca
     1261 acgacaagaa gcgagtatta tttgacggac aagaagatct tttgactgaa cagaatcagt
     1321 ccccgtttga acagatgaaa gaatttcatt tcacgaacac gaacaccgag aaaatccgtc
     1381 gcaagcgtcg tgcaacaaat ggattgaagt cagctccgcg taaggaaaat ttggaaaagc
     1441 ataaaggaaa attaccggat tataccggcc attaccaatc agaaatcaca tcgacaggac
     1501 gaaaaaaact ttttggtgat tcctctcgtt ctgtcagaca aagtgaaaca aggacagagt
     1561 ccaagcatac tacacgaaac gcatctgttg acacgaaggt gcgggagcgt tcttattttg
     1621 ttcctaaata tgtacccgca tcgattatcc cagaccctag agaaagcaaa atttctgagg
     1681 atcatttaat cgaatcgatg aaaaaaacgg atgactctta tctgttgttc gatacagaac
     1741 cgacgccgta tcaagaaaaa agaaatggtg agccgtctgt acgaacattc aatcgaacaa
     1801 aaacgttaga ggaaacaaag cagtcagaag aaaaaaaatc gaaaaaaagc catggtatct
     1861 tagaacgctc attacaagga ttgatcgatg atcaaggaaa ctctttggga gaaaatagct
     1921 attttaaata agtgatatac aaatttatat tcggctacag ttggaaagga aacgctgcgg
     1981 cgtttcctta ttttatactc tatccgaaag tttatatcgt atcctcaaat ttttttgagt
     2041 ttaagcgaat tttttctccc aataagtaag aaaataatgg caaaataaaa aaacaaacta
     2101 ttttcgcaga tcgtttgcaa aaatagttt
//
LOCUS       DAGCYL010000239         2120 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.247, whole genome shotgun sequence.
ACCESSION   DAGCYL010000239 DAGCYL010000000
VERSION     DAGCYL010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.247"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(83..1009)
                     /gene="cysK"
                     /locus_tag="MB216_002658"
     CDS             complement(83..1009)
                     /gene="cysK"
                     /locus_tag="MB216_002658"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBT4527434.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1149..2036)
                     /locus_tag="MB216_002659"
     CDS             complement(1149..2036)
                     /locus_tag="MB216_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBT4527435.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          584 a          474 c          356 g          706 t
ORIGIN      
        1 ttttctcatc ataaattttt gtgtgttctt ttcatcttta aaagtttttt acataaacct
       61 atctggagaa aaaccaagtg ccttatatct cttggtaaag ggctgtagat aaataacgtt
      121 caccattgtc gggaaccaca gctaatacct tcttgccttt tcctaattct tttgcgactt
      181 ttagcgcggc agcaattgca gcgccagatg agatgcctac taaaatccct tcttttcgcc
      241 caacttctct agctgtttcc atcgcttcgt ctccactaat agaaaggact ttatcataca
      301 cagaagtatc cagtgtttta ggaacaaaac ctgtaccgat tccttggatt ttgtgaggtc
      361 ccgcttcttt cccttctaaa atagcagatt ctgctggttc taccccgatt aattcgattt
      421 tcggatacac acgttttaat tctcggccag cgccagtgat cgttcctcct gtaccgatac
      481 ctgcaacaaa cgcgtccaag ccatttactc caaatgcttg atgtatctca ggaccagtcg
      541 tcttctcatg cacaagagga tttgcttcat tttcaaattg taatggtaaa aagtagccgt
      601 tttcttttgc caaacgttct gcttctctga ttgatccaga aataccatcc gcaccaggag
      661 tcaaaatcaa ctctgcgccg taccctttca tcaataaacg tctttcgata ctcatcgtct
      721 caggcatcac gatgattact ttatatcctt ttgcaactcc taccatcgac aatccgattc
      781 ctgtatttcc agaagtaggt tcaatgatag tatcacctgg tttcagtaga ccatcgtgtt
      841 ctgctttttc tatcatactt aaagcaatac gatcttttac acttcctcct ggattaaaaa
      901 actcaagctt tacaaaaaca tctgctgaat cttctggtac gattttattt aatttgacga
      961 ttggcgtttt gccaatcaat tcggtgactg agttatagat agctgtcatc ttcaattcct
     1021 ccttgtcttt gctcttctta cttatcataa tacaatctgt tcatttatgg cgagtgattc
     1081 gtctttgact agcaaaaaaa agacaaagat ataccatata tggtaatttc tttgcctttt
     1141 tatctaaatc aaacgtttag ttcttgacca actaataaag aataactgga catagacatg
     1201 ccatttttcg ccattagatc atcaacagac atgccaaatt tttgtgcaat atcccatagc
     1261 gtatcacctg acacgactgt atatgtctga ccattatttg tatttacatc tgttgttgaa
     1321 gatgatgacg ttgaagtcgt cgtagtagtc gttgttgttg tacttgtggt tgttgtagcc
     1381 gaacctgttg atggcgtatc aaaacgggtt aaattataag tattaatcaa tgagttcaat
     1441 ttggccccat aatttggatc ggtagcataa cgacctgtca agtacgctgt tgcatcataa
     1501 aaacttgttg tattgctttt ccacacgcca gcataatgat aatcgcccgt ggaaaaactg
     1561 gttgaacgaa gaacattcgc atggtcttgg aaggattccc aataagaaga atattttcgg
     1621 aatggctcat tcatcgttac ccattgtccg tttacatatt ctttggttgc catataaacg
     1681 acctgtccgt tatagcttcc tttcacaccg aatagattat aatttggcgc actagctaat
     1741 ccggaagtgc catatgcact ttccaacaac gcttgagcga tcatgactga agcatacaag
     1801 tcattacttg cagcaacttc tgacgcagac cagccgattt gatcgataaa cgcttgagga
     1861 tcagcgactg tttgagatcc ttgttcatct gcatgaacat gttgcggtac cgctgtaacc
     1921 atcatcggcc caactaaaag actcgttcca attaaaggag caatcgcttt tttattgatc
     1981 accatttttc ttttaggtct atggtgccgg gcactgcggg tcttcagctc ttgcattcaa
     2041 attcctcctc aaaataaaaa aacttaataa aaagttctct tcttaaaaca aacttaatta
     2101 attaaattat acttttaatt
//
LOCUS       DAGCYL010000240         2104 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.248, whole genome shotgun sequence.
ACCESSION   DAGCYL010000240 DAGCYL010000000
VERSION     DAGCYL010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.248"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            169..1539
                     /locus_tag="MB216_002660"
     CDS             169..1539
                     /locus_tag="MB216_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HBT4527436.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="MB216_002661"
     CDS             1560..1844
                     /locus_tag="MB216_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527437.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DAGCYL010000241         2095 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.249, whole genome shotgun sequence.
ACCESSION   DAGCYL010000241 DAGCYL010000000
VERSION     DAGCYL010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.249"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1290
                     /locus_tag="MB216_002662"
     CDS             <1..1290
                     /locus_tag="MB216_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4527438.1"
                     /translation="SLISLSIKDATDRASKETFANITNSFSMEINRQVNPGTPRGGGN
                     VKGEDIKKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLT
                     GVNDSTKETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFD
                     ADNEKGADETVEVEIKGLFDGHNSGVVSAAQELYENTLITDIHTAAKVYGNTEDTAVY
                     QDATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFF
                     GSLIFAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSY
                     FLAQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPK
                     FMIYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
     gene            complement(1576..>2095)
                     /locus_tag="MB216_002663"
     CDS             complement(1576..>2095)
                     /locus_tag="MB216_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4527439.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT          758 a          310 c          379 g          648 t
ORIGIN      
        1 agtctcatta gtttatctat taaagatgct acggacagag cttcaaaaga aacatttgct
       61 aatataacaa atagtttttc tatggagata aatagacaag taaatccagg aacacctaga
      121 ggtgggggaa atgtaaaagg tgaagatatt aaaaagatat ctcaaacaga tagtatagac
      181 tcctatgtaa aaagaataaa cagtgttgca gatttagttg attatgatat tattgaaact
      241 caagagactc ttgcgaatca atcacctgaa agagcaaaga attttaaaag aacagttatg
      301 ttaactgggg ttaacgactc aacaaaagaa acaaaatttg tatcagaagc atataaatta
      361 gtagaaggaa agcatttaga aaatgaagat aaaaataaaa tcttaatgca taaagattta
      421 gctgaaaaaa ataaccttaa agttggagat aagataaaaa taaaatctaa tttatttgat
      481 gctgataatg agaaaggtgc agatgaaaca gtagaagtag aaattaaagg tctattcgat
      541 gggcataata gcggtgtagt aagtgcagca caagaactat acgaaaatac attaatcact
      601 gatattcata cagctgctaa agtttatggt aataccgaag atacagctgt ataccaagat
      661 gcaacattct ttgtaaaagg tgataagaat cttgatagtg taataaaaga tcttgggaaa
      721 ttagatataa attggagaga atataatttg attaaaagtt catcaaatta ccctgcatta
      781 caacaatcta tatcaggtat ttactcaatt tcaaataaat tattttttgg ctcattaata
      841 tttgcaggag ttgtagtttc attattatta ttcttatgga tgaatgctag aaagaaagaa
      901 atagcagtat tactatcatt aggtatatca aaattagaaa tttttggtca attcctaatt
      961 gagatggtat ttatatcaat ccccgcattc gttggttcct atttcttagc tcaatataca
     1021 gctgacaagc ttgggaataa tatattgaat aaggttactg gtgatatagc taaacaaata
     1081 gctaaacaat ctgcatctag ccaattaggt ggtggggcag aagctgaagg atttaacaag
     1141 acattatcaa gtttagatat caatgtatta cctaaattca tgatttatgt agttatattt
     1201 atgagtttag tacttcttgt atcattgatt atttcttcat tcaatatatt aagaaggaat
     1261 ccaaaagaat tattaattga caataattaa aattttcggt gctttttagc accaaaaatg
     1321 agagtgtaat ataaaccgtg taagtaacca tcaatcctag gcgggtggtt gcttcacggt
     1381 ttttattttt ctaaaataag atcaagaggt gattacacga tggctagaaa gaaaagaaat
     1441 cctgatgctg agaagttagc tgaatccatt ttgaatactt atcaacccga atctgtagag
     1501 gatatgcaag atgttttaaa agatgtgttt ggtcccttat ttgaaaaaat gcttcaagga
     1561 gaattgaata accatttagg gtatgacgcc cattctaaag agccaaagaa acatgacaat
     1621 cgacgaaacg gttatggcaa taagacgctt aaaacgagct ttggtaaagt aaacattgat
     1681 gttcccagag accgggaggc ttcttttgaa cctgagttga ttcctaatag aaaacgagat
     1741 gtctccgaca tcgcagactg tagacaaaag ctgttccaaa ctcgtaactc gagcttggaa
     1801 cagcttttta tctacttcgt ttcgaatttt tgaacaaaaa aataaaggct ccttgccttt
     1861 ccatagccag gtggctaatt tctttaaatt cattgcagca aacactagca tcgtatgcgt
     1921 cgcgactttt tccaagcctc gatacttggt ccaacgcatc ccatgctttt cttttgcatc
     1981 cgcaaatatt cgttcaatcg tttgtttccg ttttttgtaa attgatttat tcagatctgt
     2041 taggcgaaga tgttccactt catccatcag atctccccaa acatgtctcg tgatg
//
LOCUS       DAGCYL010000242         2019 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.250, whole genome shotgun sequence.
ACCESSION   DAGCYL010000242 DAGCYL010000000
VERSION     DAGCYL010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.250"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            127..1320
                     /locus_tag="MB216_002664"
     CDS             127..1320
                     /locus_tag="MB216_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4527440.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(1486..1908)
                     /locus_tag="MB216_002665"
     CDS             complement(1486..1908)
                     /locus_tag="MB216_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4527441.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT          536 a          338 c          413 g          732 t
ORIGIN      
        1 aaactaagct ggattatcta tatcggttgt ttgtgtatta tttcaaataa aaaagtcttg
       61 tttttttatt cttttttgga tatactttta acaatttagg gaaattttag gaaaaggggt
      121 ataaaaatga caacgtatca gcgaaagtta ttattatcga catcggcggg aattgcttta
      181 gagaatatgg atattatgtt tttagctttt tcattatctt caatgattac ttcttttcat
      241 attagtggta cgcaagcggg tttcatttca acgatcacta atttggggat gctcattgga
      301 ggtatttttt ttggcatcat agctgataaa tatggacgtg tgaaagtatt ttctcaaacg
      361 gtccttctgt tttcgattgc ttctttattg atgtattttg cttcaaacat ctacttggtt
      421 tatctctttc gctttatcgc aggtgtagga gcgggaggag aatacggggc gtgtatgtcc
      481 ttgatttccg aaagtttttc aaaaaaacaa attggacgag cttcttcggt tgctggtatt
      541 ggtgcgcagg tcggtgcggc tttggctgcg attttagctg ctgtgatcat tccaacgttt
      601 ggttggaaga tgttgtatgt cgtaggtgta ctgccggtgt tgatggtctt gttcattcgt
      661 cgcggcttga ctgaaccaaa ggaattcaaa tctgtacaat ctgccgaaaa gaaaaccaaa
      721 cttagcagac tgtttgaatc aaaacatctt gcttggcaaa catgtggcct tagtatcatg
      781 gtaactgtcc aaattgccgg ctattttggg ttaatgaatt ggctgccttc aattatgcaa
      841 aatcgattag ggctttcggt ttctgggtct tcattgtgga tggtcagtac tattttaggc
      901 atgtctttgg ggatgatgac ttttggcata atcatggata aattggggcc taaactagcc
      961 ttttctttgt ttttaatttg ttcatcctta tctgtctatc tacttgtgtt agcccatagc
     1021 cagtggagtt tggtagcagc cgctgtgata gtcggctact ttatcaatgg catgtatggt
     1081 ggatatggcg cagtgattag tagtttgtat ccaacagaaa tccgcgcgac ggcgaataat
     1141 ttcattatga accttggacg agctgttggt ggtttttctt ctatcgtcat cggtttcttg
     1201 atggatcatt atagtttaac tgccgtaatc ttgtttttga gcggtattta cctagtcagt
     1261 ctgctggtct tgacaactct tactggtgtt acagcattaa gggaaagctt gcagcattga
     1321 tagttgagtc tgtaaatgac aagaaagagg tgtggcaaaa gcttgcatct gcggtaataa
     1381 gaccagccaa atcaaaaaca tgagacactg tttccgtttg gctggtcgta ttacttgatg
     1441 cagggcagct ttttcaactg ctaatttcct tctatctggc ggctactatt ctttgacgtc
     1501 cgtcatttca aagtacttta ctaataactt agcataacga aaaactgtgg atacactcgt
     1561 atattgagcg tctgcataat cttccaaatt tttctcagga tgttgataca tgaattcaat
     1621 caaatgataa ttatatgatt ttctgtagaa atgaagataa ataatgtaga ttgggaagtt
     1681 ctcctgcttt tccatagaaa agcgccgatt attctcattc ttcaaaatga ccttatcagg
     1741 atatttttgg aacaattctt ctaaatctgt tagataattc aagatagact tacttgatac
     1801 cttcaattcg ttttgtagtt cttctattga acaccaacgt tctgtttcaa gcagtaaatg
     1861 aatcatttta attttctttt gagaaacttt tgttaaaaaa gtctgcatat tttctcctcc
     1921 tgtatctact ttactatact acatacaaga aaaaaaacgg atacaaatat catattgtca
     1981 caccgtcaat agtgcataaa cctgttatta tttctcttt
//
LOCUS       DAGCYL010000243         1995 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.254, whole genome shotgun sequence.
ACCESSION   DAGCYL010000243 DAGCYL010000000
VERSION     DAGCYL010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.254"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(63..356)
                     /gene="rpmA"
                     /locus_tag="MB216_002666"
     CDS             complement(63..356)
                     /gene="rpmA"
                     /locus_tag="MB216_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBT4527442.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(369..713)
                     /locus_tag="MB216_002667"
     CDS             complement(369..713)
                     /locus_tag="MB216_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBT4527443.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(740..1048)
                     /gene="rplU"
                     /locus_tag="MB216_002668"
     CDS             complement(740..1048)
                     /gene="rplU"
                     /locus_tag="MB216_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBT4527444.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(1068..1144)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            1330..1971
                     /locus_tag="MB216_002669"
     CDS             1330..1971
                     /locus_tag="MB216_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBT4527445.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
BASE COUNT          602 a          436 c          351 g          606 t
ORIGIN      
        1 tttataaaca aaaaaggctg tgacaaaagt cttgtcacaa cctctttaat cataagattc
       61 aattaagctt cttgagctac tggataaaca gacacttgtt ttttgttacg gcctttacgt
      121 tcgaaacgta ctacgccatc aacttttgca aataaagtgt cgtctccacc gatacccacg
      181 tttacacctg gatagatttt tgtaccgcgt tgacggtaaa gaattgatcc acctgtaact
      241 gtttgtccat cagcacgttt agcacctaga cgttttgatt ctgagtcacg gccgttagat
      301 gtagaaccgc cacctttttt gtgggcaaat aattgtagat tcatagataa caacatggtc
      361 tgcacctcct atttatcatt aattgtttta atttggatat attctgagtt ttcagcttct
      421 atcgattgta atcctaataa aagattttct aatagaatct cagcaatatt gttttgttct
      481 tgtgtcattc ctgaagtgac ttcggtataa agataaccgc cttcttcttc attcatttca
      541 acaatcggct caaaaccggc taatgaggca atcccgttga cagtgctgat cgctaacgca
      601 gaaacacccg cacaaacgat atcacttcca taaggaccag catcagcatg cccggtcaaa
      661 gtgaatgaaa cgatctgacc tgcgtcattt cgcttaaatg ttcctttgat catatatagg
      721 ccttctttct caaacgctat taagcgttga tcgcgtcgat aaccactttt gtatatggtt
      781 gacgatgacc ttgtttacgg tgtgaatgtt ttttaggttt gtatttgaaa gtaacgactt
      841 tcttttgttt gccgtgtttt tcaacagtac cttcaacagt tgcacctgca acagttggag
      901 cgcctacttt tgtagattct ccgcctacta agataacttc gtcaaaaaca actttttctc
      961 ctgcttcaac gtcaagtttt tcaacataga ttgcttgtcc cacttcaact tttacttgtt
     1021 taccaccagt tttaataatt gcgtacatgc tataaagcac ctccttctga tatacttaga
     1081 ctcgccatcc gaagtgacag catatgctgt gcttattact tgttctgagc ggttgtagct
     1141 gtggagtcca caattacaac agtactagct taccagaaca gcaaccgtta gtcaactaaa
     1201 tttaaaatca aaaagtaaaa caaaaattca agcaatactt ctgcttttct atcctcacag
     1261 ttctaccaaa taaaatagtc gcattgtgtt tcttttattc ttataataaa agaaaaagga
     1321 ggagcataaa tggaataccc aaaagaactg atacaagaag cttctttaag aatgaacggc
     1381 cgtcctgtag aaggatttat cgctgggcaa ggacctcttc atcctaaact gatgctcgtg
     1441 ggagaagccc cagggaaaac agagattgat acgcatgttc cttttagcgg tcaagctgga
     1501 aaagaattga tgcaggcact ttcttcaact ggcttaacac gagaagaggt gtatatcacc
     1561 agtgctgtaa ggagccgccc gtatcgcgtg actcatcgga tcaataaacg gacacaacag
     1621 tcagaaactg tttatccaaa tcggacacct actcgttccg aagtatttgc gcatgcacca
     1681 atattggatt acgaactgat gaaggtccag ccaaaattaa ttgcgacact tggaaacatt
     1741 ggtcttcaac gtttacttgg caaagaatat acggttacca aaaatcatgg tcaactctat
     1801 acagggcctg tcttacaatt aacagaacaa aaggatgcct acgaatggtc agaaaaaaac
     1861 tatcgaattc ttccactttt tcatccagca gctattttct acaatcgctc ccttgtacct
     1921 gttatccatc aagactggca agtactagct tcacttttac agcaatatta aaaagaacag
     1981 acaagaccat aaaat
//
LOCUS       DAGCYL010000244         1954 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.255, whole genome shotgun sequence.
ACCESSION   DAGCYL010000244 DAGCYL010000000
VERSION     DAGCYL010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.255"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            168..1034
                     /locus_tag="MB216_002670"
     CDS             168..1034
                     /locus_tag="MB216_002670"
                     /inference="COORDINATES: protein motif:HMM:NF025663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527446.1"
                     /translation="MNIHNIGKKILCRTFPIIMTKYFYRKILGRKLKLKKPETFNEKI
                     NWYKLYFCPQNDLVIQCTDKFRVREYVEDMYCGDSLNDLIGVWDGVDEINWNSLPQRF
                     VLKCNHGCGMNIICPDKTSLDIEETKNRLNKWMKMDWGKESIEPHYSKIKPKIICEKF
                     IENTLDKESSLPPDIKIHCFNGIPKVIAYYSGRGNDMHGTFYDVNWNKLDFATKEGSV
                     ISKPICLDKMLLYAEKLSNPFPYVRVDFYECNNIPVFGELTFTPASGRSPYMTREADE
                     YMGNLLNLKEME"
     gene            1036..>1954
                     /locus_tag="MB216_002671"
     CDS             1036..>1954
                     /locus_tag="MB216_002671"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HBT4527447.1"
                     /translation="MRTKKAFYNTTTGIIYQLCSIVCGFIIPRLILGYFGSEYNGMIS
                     SITQFLGWISILTAGVGGVTRAALYKPLNKNDNYKISGIINATDNFMKKIALIFLGGL
                     IIFAIGYPFLLKNKFEWFFVFTMVLVLGINTFAQYFFGMTYQNLLEADQRQYIYSTVR
                     ILTTLISTLVTVVLIIMHFDIRIVKLASSLIFAVNPIFLNIYVKKYYSIDTKIAPDNS
                     AISQRWHAFAQEIAVMINNNTDIVVLTIFSNVLEISVYVIYNMVLSGVKQLLQTFTTG
                     IGAAFGSMIAEGIDNSSLNRKLKVFEVLVY"
BASE COUNT          719 a          221 c          341 g          673 t
ORIGIN      
        1 gagtttaaag cagcaaaaaa gtttaaaaat caatatcata ttaatatatt cacatcatta
       61 aaagacttag ttatccatgt aataaaaaga tactctattg cgttgaaaaa aagaacattg
      121 tcttatgaag aaatgttggt agaattagaa agaggaatga attactaatg aatattcata
      181 acataggaaa aaaaatactt tgcagaactt ttcctattat catgacaaag tatttttata
      241 gaaaaattct tggaagaaaa ctgaagttaa aaaaacctga aacatttaat gaaaaaataa
      301 attggtacaa gttatacttt tgtccacaaa atgatttagt aatccaatgc actgataaat
      361 ttagagtaag agagtatgta gaagatatgt attgcggtga ttctctaaat gatttaattg
      421 gagtatggga tggagttgat gaaattaatt ggaactctct accacaacgt tttgtgttaa
      481 aatgtaatca cggatgcgga atgaacatca tttgtcctga taaaactagt ttagatatag
      541 aagaaacaaa gaatagatta aataagtgga tgaaaatgga ttggggaaag gagtctatag
      601 aacctcatta ttcaaaaatt aagccaaaaa ttatttgtga gaaatttatt gaaaatactc
      661 ttgataaaga atcttcttta ccgccagata taaaaataca ttgttttaat gggataccta
      721 aagttattgc gtactactca ggtagaggta atgatatgca tggaacattt tatgatgtta
      781 attggaataa attagatttt gctactaaag aaggttcagt cataagtaag cctatttgtt
      841 tagataaaat gttattgtat gcagaaaaat tatctaatcc tttcccctac gttagagtag
      901 atttttatga atgtaataat attcccgttt ttggagagtt aacttttaca cctgcaagtg
      961 gaagatctcc gtatatgacg agagaggcag atgagtatat ggggaatttg ctaaatttaa
     1021 aggaaatgga ataaaatgag aacgaagaaa gctttttata atacgacaac tggaatcata
     1081 tatcagttat gttctattgt atgtggtttt attattccaa gactcatttt aggatatttt
     1141 ggatccgaat ataatggtat gatttcatct ataacccaat ttttaggatg gattagtatt
     1201 ttaactgcag gagttggagg agtcactaga gctgctcttt acaaaccatt gaataaaaat
     1261 gataattata aaataagcgg aataattaac gcaactgata attttatgaa aaaaattgct
     1321 ctcatatttt tggggggact tataattttt gctattggtt atccatttct cttaaaaaac
     1381 aaatttgaat ggttttttgt tttcacaatg gtattggttt tgggaattaa tacttttgct
     1441 caatattttt ttggaatgac atatcaaaat ttattagaag ctgatcaacg ccaatatatt
     1501 tattctactg ttagaatatt aacaacactg atcagtactt tagttacggt tgtattaatt
     1561 ataatgcatt ttgatatacg aattgtaaaa ttagctagtt cattaatttt tgctgttaat
     1621 cctatttttt tgaatattta tgttaagaaa tattacagca tagatacaaa aatagcaccg
     1681 gataattcag cgatttcgca aaggtggcac gcttttgcac aagaaattgc agttatgata
     1741 aataacaata cggatattgt cgttcttact atattctcaa atgtattaga aatttctgta
     1801 tacgtaattt ataacatggt tttaagtgga gttaagcagt tattacagac atttactaca
     1861 ggtatagggg cagcatttgg aagcatgatt gcggaaggaa tagataatag tagcttaaat
     1921 cgtaagctta aagtttttga agtactcgtt tata
//
LOCUS       DAGCYL010000245         1953 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.256, whole genome shotgun sequence.
ACCESSION   DAGCYL010000245 DAGCYL010000000
VERSION     DAGCYL010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.256"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..734
                     /locus_tag="MB216_002672"
     CDS             <1..734
                     /locus_tag="MB216_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBT4527448.1"
                     /translation="ALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVV
                     GLLSIIAMYYVTDITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSN
                     LRFELWKSAIEIFKSKPISGGSPGYYIEYAHNKLPYTLMGKDTLTAHNFIFLVMAATG
                     GLGLLTLLTFLFIQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVST
                     IGSFIFWGYLGSIQKTISKEEGVTN"
     gene            868..1812
                     /locus_tag="MB216_002673"
     CDS             868..1812
                     /locus_tag="MB216_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBT4527449.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
BASE COUNT          669 a          281 c          359 g          644 t
ORIGIN      
        1 ttgctttatc gggttcacgc accacattag tttgtttatt aataacaact tttgttttta
       61 gtttctttac aagttatagt agtcgttttc ttaaaactaa tactttaaaa agatggctaa
      121 ctgcattagt agtaggattg ctaagtataa ttgcgatgta ttatgtaaca gatataactg
      181 ctcatgcatg ggcacatgtt ccgcatatta cagaagtaaa aaaagataca tctgattcat
      241 caggaacaac tagcagcaat aaagttgatt taaaacgccc tgacactgat agcggggatg
      301 tttctaattt aaggtttgaa ctttggaaaa gtgcaattga aatattcaag agtaagccga
      361 tatccggagg ttctccaggc tactatattg aatatgcaca taataaacta ccatatacat
      421 taatgggaaa agatacttta actgctcata attttatttt cttagtgatg gctgctacag
      481 gaggattagg tctattgaca ttgttaacct tcttatttat ccaatgcttt aaagccttgc
      541 gttattgttt cacacaacca aatctagtaa aagataagca cttttttgct attttaatga
      601 ttcttacaat agcagtttcg gcattattta tcacagaact cattttggtt agtacgatag
      661 gttcatttat tttttgggga tatctgggtt ctatccaaaa aactatatct aaagaagaag
      721 gagtaactaa ttgaaactat ttttaggtat atgaaagtag aaaagaaaac tagttatccc
      781 aacttcaaca aattttatag tttttacaat ataaaaatgg taaactaata ttatttgata
      841 cgaaaatgtg caattggagt gaatttgatg gacactatat tgattactgg cggagctggt
      901 tttatcggtt caacgctagc gaattatttg ggaaaagaaa acaaaatcgt agtagttgac
      961 gatttatcaa tgggaaaaaa agaaaacttg gatatggata aacaagtgac tttcattgag
     1021 ggggatgtat cagattctca attgatggaa agaatcatga aagaatatca gtttgcctat
     1081 atttttcatc tagctgctgt agctagtgtg gcagattctg tagaacggcc tgtagaaaca
     1141 catcgtgtaa attttgacag tgcgctttta ttgcttgagt tagcacggaa atatcaatct
     1201 aatttaaaac gattagtatt tagttcatca gctgcagttt atggtgatga accaacactg
     1261 ccaaaaaaag aagaatcagt catccgtcca ttaacaccat atgctatcga taaatttgca
     1321 gcagaacaat atgttttgga ttactgtcat ttatatgatg ttccgactag tgctgtccga
     1381 ttttttaacg tttatggtcc aaatcaaaat ccaaactctc catatagcgg agtcatttcg
     1441 attctagtgg atcgttacaa aaaacaattg gcaggagaaa aagcggaatt tactttattt
     1501 ggcgatggga gtcagtctag agactttgtt tatatagaag atgttataca agcactttta
     1561 cttgttgcca aggaagaaaa agctttagga cagcaattca atgtcggtac aggaaaaagc
     1621 actacgctac ttgaattgat ccattcaatt gatcaaatct taggaacaga gctagcgctt
     1681 aaatatgaag cggagcgttc aggggatatt cgtgattcct tagcggatat ttcaaaaatt
     1741 cgttctttag gctatcagcc taaatttgat atattgagcg gaatggaacg gtatttaaag
     1801 acagaaattt gatgaaaaaa tgattgcatc tttggataaa taattctaaa aagtaaaaca
     1861 agaattaaag ttgtataacc aactatttaa ttcttgtttt actttttatt taggaaatat
     1921 ataattaaat attttacatg aaaaagagaa ctt
//
LOCUS       DAGCYL010000246         1946 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.257, whole genome shotgun sequence.
ACCESSION   DAGCYL010000246 DAGCYL010000000
VERSION     DAGCYL010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.257"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="MB216_002674"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="MB216_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBT4527450.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="MB216_002675"
     CDS             852..1040
                     /locus_tag="MB216_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBT4527451.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="MB216_002676"
     CDS             1053..1610
                     /locus_tag="MB216_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBT4527452.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="MB216_002677"
     CDS             1642..1884
                     /locus_tag="MB216_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBT4527453.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          715 a          302 c          401 g          528 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcg
//
LOCUS       DAGCYL010000247         1941 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.258, whole genome shotgun sequence.
ACCESSION   DAGCYL010000247 DAGCYL010000000
VERSION     DAGCYL010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.258"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            801..1274
                     /locus_tag="MB216_002678"
     CDS             801..1274
                     /locus_tag="MB216_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBT4527454.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1283..1510
                     /locus_tag="MB216_002679"
     CDS             1283..1510
                     /locus_tag="MB216_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4527455.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          626 a          322 c          295 g          698 t
ORIGIN      
        1 tgtactattc attgatcata acgattggct atttgattgt atcaaatcta ggaaatatgg
       61 tatttggtat cagtaaagaa ttcagttgga ctactacatt atgggaatct ctattctttt
      121 tcatcttcgt atttttgtta caaaattatc gaaaaaaata aacatgtgcc atctatctgt
      181 tggcacatgt tttaggattt atggtacttt ttattcggca atccatttta tttttctact
      241 tcttcttaat acctctaaca cttttgattt ataaactaat ccaatgcatc tacttgcgct
      301 tattaagcta tttcagctag atagtaccac cccatgcgtc aatgcttgat tttccttttg
      361 tataacattg tataagcaat aaaggacaaa taagtggtga ttacaccaaa ccattgaata
      421 agaaaaattc tgatacgtaa ataaacatga acttgaaaaa attaatgaat cggttagcta
      481 ctacgtagtt gtcttttttc tcatacagtt ctgttaactt acacttctct tcgctagata
      541 ataaaaaatc ctccaactgt attgtccaat ataatcccag gcatctaaac tagcattata
      601 gaagagacac acatagaatc taaaataact ggttttaaat gtttcttaca atcaaaaaaa
      661 gctgttaaat gcactccaca ccttcatact ctatcatttt ttataaacga tatattttta
      721 ttgtttttcg ataaaaaata gttgttttac aataattatt ctgctatatt ttagctatca
      781 aagataaaag agggataaaa atgaaattta aaacattgct attaaaattt gtcgttttag
      841 gtataagtgc cgtatttatt ttgttcggcg tactttttta tactcagatc gctacaagcg
      901 aaaaaggatt caattttgat tggcagacca cattatttgt ttctgtcatt tactttgccc
      961 ttctcctagg gatcgttatt gcttatttct tattgaaact attgaaaaaa atcgaagata
     1021 accaagcttt ttccactcaa agcttaaaca tactaaaaaa aatcgaacga accattttgt
     1081 ttattggttt cgcctttttt ggcttacttc ctaaatttat gaaagtagcg gatttagacg
     1141 atgctccagg tttgatgttg gtaggaatcg ctgtcgtatt cattcctttt gcagtataca
     1201 cattaacaag cgtgctggaa aaactgcttg aacatgcgat tttacttaag acagatcatg
     1261 atttgacagt gtgaggtgcg acatggcaat tatcgttaat ttagatgtag tattagctca
     1321 aaaaaaaatg agtgtgactg aattgagtga gaaagtgggc attacgatgg caaacatctc
     1381 tattctaaaa aatggaaaag caaaagccat ccgtttttcc actctcgaaa aaatctgcca
     1441 agttttggag tgtcaaccag gtgatttgtt gacctataaa aatgactcga atactatacc
     1501 agaagaataa aataaagtag aacaaacagt aaataaaaaa taatctgaat gatagatgtg
     1561 tgagactttt agaagttatt tacgtctatt attcagattt tttatttcga ttgaataccg
     1621 ttttatctat aaataagata tgtactgact catacaaagg gggccttttc ttctccatgt
     1681 actatcactt ttcctgatac aactggtttc ctgttacgtt gtagttggat ctgcttttag
     1741 tataatactt tattcattcc accagactga tagcagtttg agcagtctaa ataatcatgt
     1801 agtattacag gccattctgt ttcatacctt gaagtgctta aaacttcagc taatggccca
     1861 ttgtgttaac tcttactcca ttctttttta gtaagctccc atatatactg taaagccttc
     1921 tatatattat gtcatgttgc t
//
LOCUS       DAGCYL010000248         1928 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.259, whole genome shotgun sequence.
ACCESSION   DAGCYL010000248 DAGCYL010000000
VERSION     DAGCYL010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.259"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..367
                     /locus_tag="MB216_002680"
     CDS             <1..367
                     /locus_tag="MB216_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBT4527456.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..770
                     /locus_tag="MB216_002681"
     CDS             567..770
                     /locus_tag="MB216_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527457.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            1330..>1928
                     /locus_tag="MB216_002682"
     CDS             1330..>1928
                     /locus_tag="MB216_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBT4527458.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKF
                     HPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIV
                     DPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          625 a          293 c          454 g          556 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggttcacct
      661 tagggtttag tatttcgttc aaaaagggaa caaagaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaatg gtttgtctgg tagaaaataa ttagcagatt
      781 tacaagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactac attttctaca
      841 atagctctaa aatggcatat tttgcgttct gagcggtttt agcgtttatt tcgtttagtt
      901 atcggcacaa tcggtgaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactggt
      961 agcggaacgg tcgtgttaga ttgtgtagcc gatagctgaa cgaaataaat gctggcagcg
     1021 ccattctatt tcggcaggag gaggctcaag gggttcgggg aatgaaattc cctgaggtct
     1081 ttttattttt tctagaggag tgctataatc aaagaaaatg agaaaaaaga gtaaaaaaat
     1141 agtcgagcag tgttgacgca ctctcgacct ttacggaaac tacttcacct agttccgtaa
     1201 ctcaagttca ttatacgaat atttttaggt ttcttcaagc tagagatttt tggatgtgga
     1261 tttttagagt tacgtgaact ctggaaattc tttccaagaa tctctttttt cgttgaggag
     1321 gagctatttt tgagtaaaaa aggcataatc gtaaatgagt gggaaaaact taaccgtatt
     1381 tctaacaggc ggttaagtgt tgacgataag gtacaccccc taaaattaag ttggtcaatc
     1441 gatcgaatta caatcgtcgg aaaactgaat gataccatag cttattggac gcaaaatgga
     1501 gttgtgtttg tcgattttga gcaattaatg cgtttgaatg aaaatcaagg gtatttgaag
     1561 tcggttggta caaatggttg gcaattattt gaccaatatg gagaagatat cgcctatatc
     1621 gaaatgttaa aatttcaccc tgacaaaggt cgtattgatt ttaatccaaa taaaattggc
     1681 gagtttttaa aggggtcgat gaaacaattc attcatgatt tgtttctgga tgcgcatttc
     1741 tcacgtgcag atattgcttg cgatattctc ggtgttcctg atgattttat tacccagtat
     1801 cgaatcgttg accctgtgtc ttttaagcca atttatggcc gttctggcaa attagaaaca
     1861 gcgtactggg gtagtcgtgc cagcgagcga caagtgcgga tgtacaacaa aaagcttgaa
     1921 caggaacg
//
LOCUS       DAGCYL010000249         1916 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.260, whole genome shotgun sequence.
ACCESSION   DAGCYL010000249 DAGCYL010000000
VERSION     DAGCYL010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.260"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(599..889)
                     /locus_tag="MB216_002683"
     CDS             complement(599..889)
                     /locus_tag="MB216_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527459.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1097..1789)
                     /locus_tag="MB216_002684"
     CDS             complement(1097..1789)
                     /locus_tag="MB216_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBT4527460.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          524 a          370 c          292 g          730 t
ORIGIN      
        1 aaaagaaatc tacgatagtc aacaaaaaaa ttttcattat atccagaaaa atttttctta
       61 gttaaggacc tatggattgc agtaatgcag aattcgtcga ataaactgcc aaagacatac
      121 aatcatattg cttttaaaat tgacgaacaa gatatagatt cgtttgtctc aaaaatccaa
      181 atgttaggtt taactgtcga accaggacgt tctcgtgtga aaggcgaggc ttcttcaatt
      241 tacttttacg actatgataa tcacctcttc gagttacata ccggtactct ccaggaacgt
      301 ttaaaatcct acaattccac tacctgagac tagtaatgac tagtctcttt ttatggcctc
      361 tgatcccccc attgagcgaa tactaatagg gcttcgaatt gatctgttca atctttaagc
      421 aatatccctc aaatgagttt ggaaagttta gtgattcatc acaaatactt aaatacatgt
      481 agttatatat agattgaggt tcttgtgagc gtatgctcgt gtgggttcaa atcccatcgt
      541 ctgcatccat ctttccaaaa caagcggcta ggacattttt gtctcagccg cttgttttct
      601 agtttattct ttcatcttgt cggacctttt ttcttgggta tagacttttc tcatattcta
      661 ccacaatttt gggtttccct aacacatgat aggatctcac aacaaattgc tttctattat
      721 tataataacc tgtaacgact aacttcatcc cttcgtctac ttcagctagg aagttcaaac
      781 tatgggaagc aatcaaacaa ttgaccccat ttaaactgaa acggatcaac ggtctggcac
      841 taacttgcaa agtacggatc ttatctactg ttccatattt tttctccata accttcacct
      901 caataccatt atacgaacat acattccctt tgtaaagaag aaactctgtt ttatcttttt
      961 gactttttgt ctattacctt tcttaaaatc atattggcac ttcctgcgaa gatgaaggac
     1021 atgtactaaa aaaaagaggc agcagtatag attactgttg cttctttact aatttaggtt
     1081 tacttgttaa gaaagattat ttacgcaaat gttcttcaac aatttgctga gatttatcag
     1141 ctgtatcttc cacttcatct tttgcttttt ctaacttttg aatatattgt ttcaagcttg
     1201 agttttcagc agttgcttcg tctaatttct tttgtgtatc tgttaactga gtttctacat
     1261 ttgttttttc ttgtgtcagt gttgcgattt gttgattcaa accatttaca gtattttgta
     1321 attctgaaag ttttgcttgt gcttcttgta gtcttgctgt ccaatctgca ttcagatgat
     1381 tgatttcatc atttttttgg ttgatctcat ctagtttgac attgatttca ttttgtttgc
     1441 tttgtagttg tgcatccttg ctgactaatt gactttctaa gttttgtttt tctgctgccg
     1501 attgattttt ttggttttct aattcattta cttttgtttg gtattcttgg acatctgcct
     1561 ttgaatcttg caatgtctgt tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt
     1621 gctctttatc tgtcaatgta tgatcaagct gtgtcagatt ctcattgatt gtatcaatat
     1681 ctgtctctcc accccacaaa ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta
     1741 gacatagaaa tgcaacgaca atcccaccag taataaattt ctttttcatt tgcatagctc
     1801 cttaatccaa tacttttaca tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt
     1861 ttaaattaca tatgtaattt taatataaat actatcaaat actcatttaa tcctcc
//
LOCUS       DAGCYL010000250         1900 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.261, whole genome shotgun sequence.
ACCESSION   DAGCYL010000250 DAGCYL010000000
VERSION     DAGCYL010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.261"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            513..845
                     /locus_tag="MB216_002685"
     CDS             513..845
                     /locus_tag="MB216_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBT4527461.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1464..1805
                     /locus_tag="MB216_002686"
     CDS             1464..1805
                     /locus_tag="MB216_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBT4527462.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          659 a          256 c          410 g          575 t
ORIGIN      
        1 aatacgaata ttattcgctt acattttttg tataaattta attgtctgct atgcttgtga
       61 tgaattgatt ttcgttctgc acttttggat gataaatttg gacaattgaa aatacaaaaa
      121 aaagaaaggc ggtatgaact cggaaggctg gtaaccttcg tcagcgttca tacacggcat
      181 taagatttgg cccttaatgc gttaaccttt cagtgttacg gtattacccg caatggattt
      241 agtataacag atattcaaaa gtgataccac tttttttggc tgtgttaagg ttaaaaatac
      301 gtgatattac gatgaatcta tgcctaagca caatgttata aaaatgtccg tgtggctctt
      361 tgccgtatga cgaacgctga aattagaggg aacgcatgtt tggatgcgtt ccctcttttt
      421 tgtttgtcat gaacgaaaat caattctcta tttttgaaaa ataacaagaa aaacttgtta
      481 aaaatattta aagaaattgg gtggtttatt ttatgtcata tacaggaatt ttgagtctca
      541 aagatatttg tcactacggt aaacggtgta ccgctacaga gaaaatcacg aagaaactat
      601 caactggcca aagtaaaacc gttgtgcagt gtaagaaata tataatccag aaagacaaag
      661 tctcagagga aatgatttat tacattggga aacaaaagca aataatttta aaagatccta
      721 tccccttaaa agagctatat ccaacaatca aacatgttta tgaccaaaac ggtgtactga
      781 ttggtcgaag aaaaaatgga gtcttgcgtt gtaccgcaaa agggatgggg cgtttgatcg
      841 gctgaattga tcattctcat aacttgtatt ttatcgtttt tcgcttgctt gaaaatattg
      901 tcgagacttc taaaggaact tagagtcact agaaaaataa ggagtagact tgatctatca
      961 acaattccct tcggggagtg acggggtagg ttttaacgat tggtaacgta aaaaattggt
     1021 caatcgagta aatgcgcttg tttggatagt ggtgaaatga atttgtgtaa ataataagcg
     1081 cagagtaaac aagagggaat ttagaagtta gacaagttag aatagcgtct agattaatca
     1141 tgagcagcta ctaagggatc tagactttga cgcaaactca tggaataagg agtaggtctt
     1201 ctcgtaatgg ggaggacgca caagaaattg tgtcgtgtgc tgcagtacat gtagatactt
     1261 gcgaggacgt tgattattgg atcaatcgag catttaccgc tgtctactag ggtttctcgt
     1321 ttcggacgcc ataaacgagt cgaaattttt tatgtatttt taagtaaacg aaaaacgata
     1381 aaatatgaac aagtcctgaa taaagtgaaa aaattgatat aaaacataga gaaaatgaaa
     1441 tttacgtaat ggaggaatga caaatgaaaa attatgatcc gaatatacga ttgggcacac
     1501 atacgattaa ggtttctttc caacgttggg attacaaagg atttgtgacg tttagaagag
     1561 gcggaaattg taaaggatta gatgttttag cattagacga ggatgattta tacgatcaaa
     1621 cattgactga taatccaatt ggattcggct tgctacctga agatgatgaa ggtaatgaat
     1681 ggtttaaaat gacgcttatg aatgataatg gagacgaatt atctgttgaa gatacttggt
     1741 catatttgag tgattatatt gtcagctttg aaatcattga atttgtagcg gataaagaag
     1801 aataaaaact ggaggaataa attttacaga taaaggctta gaaggagtca cgatgattaa
     1861 taaaaaggta gtattacctt aggcattgag taaggaattt
//
LOCUS       DAGCYL010000251         1850 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.262, whole genome shotgun sequence.
ACCESSION   DAGCYL010000251 DAGCYL010000000
VERSION     DAGCYL010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.262"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            123..1268
                     /gene="tgt"
                     /locus_tag="MB216_002687"
     CDS             123..1268
                     /gene="tgt"
                     /locus_tag="MB216_002687"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBT4527463.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1365..1745
                     /gene="yajC"
                     /locus_tag="MB216_002688"
     CDS             1365..1745
                     /gene="yajC"
                     /locus_tag="MB216_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBT4527464.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
BASE COUNT          638 a          310 c          410 g          492 t
ORIGIN      
        1 aaggataaaa aaaagaatat taaataaagt agaaggaagc cgagggaact tgcaaaaagc
       61 acttgaaacg attatgatgg tgggagttgt caagatattt tatatagaaa gtagggaaat
      121 gaatgacgca acctgccatt cgctatcgtt taatcaagaa agaaaaacat actggcgcgc
      181 gtttagggga attgatcacg ccacacggaa ctttccccac accaatgttc atgccagtag
      241 gaacattagc tacagtaaaa acaatgtccc cagaagagtt gaaagaaatg ggtgctggcg
      301 ttattttaag taatacatat catttatggc tacgccctgg tgaagactta gtagaagaag
      361 ctggcggact gcataaattt atgaactggg atcaaccgat tttaacagat tcaggcggct
      421 ttcaagtttt ttcattaagt gatatgcgga atatcgaaga agaaggcgtg catttcaaaa
      481 atcacttgaa cggttcgcgt atgtttttat caccagaaaa agcaatcaac atccaaaata
      541 aattgggctc tgatatcatg atgagttttg acgaatgtcc accattcgat gaaagttacg
      601 actacgtgaa aaaatcagta gaacgtacgt ctcgatgggc agaacgagga ttgaaagctc
      661 atgctaatcc agaccgtcaa ggattatttg gaatcatcca aggagcaggg tttgaagatc
      721 ttcgtcgtca aagcgcgaaa gatctagtcg ggatggattt ccctggttac tcaatcggcg
      781 ggttatctgt cggggaacct aaatcagaaa tgaatcgtgt attagaattc actacaccgt
      841 tgatccccga aaacaaacca cgttacttga tgggtgtcgg tgctgctgat tcattgatcg
      901 atggtgttat ccgtggagta gacatgtttg actgtgtctt gcctactcga atcgctcgaa
      961 atggcacatg tatgacttct caaggccgtc tagtagtgaa aaatgcgaaa tatgcccatg
     1021 atttccgtcc aattgatgag aaatgtgatt gttatacatg taaaaactat acacgtgcct
     1081 atatccgcca tttgatcaaa tgtgatgaaa catttgggat tcgtttgact tcttaccaca
     1141 atttatactt cttgttgaat ctaatgaaac aagtacgtca agcaatcatg gatgataatt
     1201 tgcttgaatt tagagaagcc ttttttgaag aatacgggtt caataaagaa aacgcaaaaa
     1261 gcttctaaag gaaaatcttt taaaaattaa gtaaaaaact agtgttatca gcatttttct
     1321 gctaaagtgt tttttgtaga tacagaatta gtgaggtgaa aaaaatggga acattaccaa
     1381 tgcttgtgat gtttatcgcg ctattagcaa tgtggttttt tatgtcacgt tcgcaaaaga
     1441 aacaacagca agaacgtcaa aacttgttag atagcatgaa accaggtgat tcagtcgtaa
     1501 cgatcggcgg attacatgga gttgtttcag aaatcgacaa tgacaaacgg acagttgttt
     1561 tagactgcga agggatcttc cttgagtacg atcgtgcagc aatcaaaacg gtcaaaccag
     1621 ggactgtcgt aacaaatgat gcaacgacta ctgttgaaac aacagaagaa ccagctgaga
     1681 cagttgaagt aaaaactgaa acaacagaaa cgctggaaga gacagaagag aaggaagaaa
     1741 aataagtatc aaagcttatt taaagacgag gtagggcaga aagatgtcct acctcatttt
     1801 ttattcaaaa aagaaagaga taagagcatg ggatatattg ctcaatattg
//
LOCUS       DAGCYL010000252         1843 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.263, whole genome shotgun sequence.
ACCESSION   DAGCYL010000252 DAGCYL010000000
VERSION     DAGCYL010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.263"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(389..886)
                     /locus_tag="MB216_002689"
     CDS             complement(389..886)
                     /locus_tag="MB216_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4527465.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(926..1735)
                     /locus_tag="MB216_002690"
     CDS             complement(926..1735)
                     /locus_tag="MB216_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4527466.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          530 a          343 c          320 g          650 t
ORIGIN      
        1 tacataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc caagtaaaaa
       61 cagcttctta tgtttttact tggctgttct tattactgca gatgcaagct tttgaatacc
      121 gtttaaggaa gaggttgtga aatcagcgtt ttgacctgag tattagctta acaaacggaa
      181 aaatagttcc tcatattttg ccgtttgtta agctaatacc gagggtcagc tgattgaaca
      241 ccatttaaga aagaggtcgt gagcctgccg tttcactctg tgtcataaaa aagccaatct
      301 actttgatag aaaaagcacg taggactaaa acagttctgc gtgctttttt tattgtgtgt
      361 taaatatcgt aaatgaagat agatattttt atgattcgac gatagagacg ttgaaatctt
      421 tcaacagctc taaagcggat tctccgtgtt ttctactgga gactaacatt ggatcaacaa
      481 aaaagtctgc ttcaggaaaa gcactctgca ggcctatcag attagagagt acacacaagt
      541 ttgtttcaat cccgactact tcgatctctt tttgttcttc tttgctttca aataaagttt
      601 cctggatttc agctaaagtt tcaggtggaa tcgtaaagta atacttttta atcactcgtt
      661 cattttcttg tggagaaaga agagggatca attgcaaatc ttctcgttct tcttctttgt
      721 ccttcagttc aactggtata tcccgtgtga agagaatata ttcattattt tggcgtgctt
      781 tagctaatct ttgagcaata cgttctgcca attgatctga ctctggaata taaaattcgc
      841 tgtttggatc aagaatatga ttttgcatat caataataac taacattttc tttcctccta
      901 cattcatatc gtcattttat tctttttaat agaagttttt taaaatataa gcaacaccag
      961 cttcttcatt cgtcagtgtt acttggtttg caatttcttt tacttgtcgc tgtgcattcc
     1021 ccatcgcaat actcaacccg ctgtaattca acatagcaat gtcgttttct tgatcgccaa
     1081 aagccatgat gtcactcgat tcgattccaa ggtaatccgc cagaaaaaca agtgaatgag
     1141 ctttagtgat acctgtaggc gtgatttcta agaaatgcgg atcagagaat actgattgga
     1201 taccatctgc ttgcagatct ctttgtagct tctgtagttt ttcttcactc ttatctgtta
     1261 atgctaattt aagatagcta agagaaggat cttccaacat atttaaaatt tgatcaaaag
     1321 aatggttctg taaattgaaa tgttcataat aagggtcatc tttgactaga tttgattcat
     1381 cgaatgtcag ctgatcatct tgggtttctc ggctagcaat gatggatatt ttcgcttctt
     1441 gagcatagaa gaaggctttt ctcagtgtat cgctatctat ttttgaacgt ctgagaactt
     1501 tcgccatttt tccatccttc atttcagcaa tcagtgaacc attggaagaa atcaaatagc
     1561 caccgattcc taattcggaa aaaatatctt tagcagaaat gctgttcctt ccggtagcga
     1621 cggttacgat tccgccattt tcatcaaatt tccgaagtgc ttggatattt tcttctggta
     1681 tctttccatt tcgatcaagc aaggtaccgt ctaaatcaat cgctgctagt ttcattttat
     1741 cttcctactt tctttttaca tatctatttt cctgttacct taagattaaa tgagaattta
     1801 aaaaaatgaa actaacttgc tttgtcatta tgaatttttt caa
//
LOCUS       DAGCYL010000253         1833 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.264, whole genome shotgun sequence.
ACCESSION   DAGCYL010000253 DAGCYL010000000
VERSION     DAGCYL010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.264"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            612..1424
                     /locus_tag="MB216_002691"
     CDS             612..1424
                     /locus_tag="MB216_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527467.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT          622 a          259 c          301 g          651 t
ORIGIN      
        1 atggcctttt tacttttaca caattataag gactttatcg aaaaaaagca gttatttgtt
       61 aaaatcattg atattattaa taataagtat tttttgaaag gggaaatata aaaggttttt
      121 gaaaataaat aaaaaatagt attacttgtt ctattgctag ttattgctag tctttattct
      181 tttttgtttt ttagggatgt acaaaagatc tcaaccacag gagacattgc ttttcacttt
      241 tctagaataa aatgcttgtc ctcagtattt tctggtccaa taaattttac cacatttaat
      301 agctatggcg gaggcgtgaa tttttttatc catatctaac atctttccag ccgtaatatt
      361 ttactggaca acaaacaatt tgattctaag ttatatactt actatacgtt gggatactga
      421 atatctgtac aattctagtg agctatcaat atggacaaaa gaatagatgc agccttctta
      481 ttcagctgtt tgtatagctt ttatggattg atatatatta gcgtactgca atttcagaat
      541 caattgcgtt gacgattgta ccagttgtct tgtattacac atatactctt ctttatgaaa
      601 gaaaaaactg cgtgattctt ttagctgtca gtatgagttt gctcatctat acccatattc
      661 tctccacctt tatgagtgtg attgttattg gcttcttgtt tatcggctct cttttctcta
      721 agcggaaaga gagaaaagaa atgtcgaatc tgatatcaaa tatgataaaa gctgttggtt
      781 ggaccattct tttaacagct tacgtttggt atccgatgtt acaacaagtc ctacatcaaa
      841 aaattaatcg tccttttcgc acaaaacttc aagaaagggc attaaacatt tctgatagtt
      901 taatcggtgc agtaaataat gatttagtta catttacaat gggaacaatt ggtgtggttt
      961 catttattct tccacttttc tttaaaaaaa aatttgccaa taaagaaaag attattaatt
     1021 tttgtgcagt gaccacttgg ttcttatcaa cgaatctttt tccatgggaa tttttacaaa
     1081 aaacacctat tcaaatcatt cagtttcctt ggagaatctt gggttttcaa gtgctttttg
     1141 gttcattgat cttaattata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
     1201 gtagccttgt aggaatagtc cttcttattt ttacagtgac tgtagcaaca gaagcgaatt
     1261 attcgcagaa aattcaatcg tataaaggtc gtttaattat gaccaaaaaa gatgttacac
     1321 tttatacaac ctcgagaact ggaggtcttt atgactatgc accattagat gcattgaaat
     1381 ataaagatca tttaaagaaa catgaagtaa aagtctatga ttgatgggag aatgcatctc
     1441 ataaagcaca taattctaaa ataatctata cagtaggaga tgcgaatgag caaaatatca
     1501 cgttacctgt atttaattac tgggaacagt tatgaaagta aatgggaaaa agagcactat
     1561 agaaaatgac aatggtttag tgtctattaa aggtacggga aaaagaacta tagttgaggt
     1621 ttcatctacg tatccaacta cgatgattct atctttatta attagttcaa taacttttat
     1681 attcttattt acacgttatt taataagatg ctgcggttaa atgaaaatag attagataga
     1741 tattataaat atgttgattc tattttcata gcaatggaga aaatacatta gcaatatata
     1801 aaagaaggta tcaagagtca cggtgaattt att
//
LOCUS       DAGCYL010000254         1809 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.265, whole genome shotgun sequence.
ACCESSION   DAGCYL010000254 DAGCYL010000000
VERSION     DAGCYL010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.265"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            2..1147
                     /locus_tag="MB216_002692"
     CDS             2..1147
                     /locus_tag="MB216_002692"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBT4527468.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1318..1776
                     /locus_tag="MB216_002693"
     CDS             1318..1776
                     /locus_tag="MB216_002693"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBT4527469.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
BASE COUNT          589 a          348 c          364 g          508 t
ORIGIN      
        1 catgcacatt caaacaaaat tgatccacgg aggcatcagt gaagatccca caacaggagc
       61 tgtcagtgta cctatttatc aaacatccac ttatcggcaa gatggcgtcg gccagcctaa
      121 acaatatgaa tattcaagat ctggtaatcc tactcgtttt gcattggaag aactgattgc
      181 tgatttggaa ggcggtgttc gaggattcgc tttttcttct ggtctttctg ggattcatgc
      241 cgttttttct ctttttcaag ctggcgatca tatcttatta ggagatgatg tctatggagg
      301 aacattccgt ctatttgaca aagttcttac taaaaatggt ctcgagtaca cgatcataga
      361 tacgagtaat cttgataaaa tagaacaatc catcaaaccc aatacaaaag cactttatct
      421 tgaaacacct agcaatccgc ttctaaagat tactgattta gaaaaaagtg ccactctggc
      481 tcatcaacat ggattgatag ttattgctga caatacattt gctactcctt attttcaaag
      541 acctttagat ctcggttctg atatcgttgt ccatagcggt accaaatatc taggcggaca
      601 cagcgatgta gtagctggtt tagtgacttc taaccataaa gatctagccg atcagatcgg
      661 attttaccaa aatgctatcg gagccgtact tggtccccaa gatagctggt tacttcagcg
      721 cggaatcaag acactaagtg ttcgtatgga ggaacatcaa aaaaatgctt ttgtggtagc
      781 tgattttcta ttttctcatc cagcagtaga aaaggtctac tatccaggat tacccgatca
      841 tgaactccat ggagttgcta agcagcagat gagcggcttt agcggaatga tttcttttac
      901 tttaaaaaat gaagaatctg ctattccatt tgttgaatca ctccaactat ttactttagc
      961 agaaagttta ggtggcgtag aaagtttagt ggaaattcct tctgtcatga ctcatgcgtc
     1021 tattccaaaa gaaaaacgag aagaggctgg cataaaggac ggcttgatcc gtctttctgt
     1081 aggaattgag tatggtcagg atctcataaa cgatttagct caggcatttg acagaatcaa
     1141 gaactagaag tcccaataaa cgacttagca gaaaaatcta aaaaatattt cagattgcta
     1201 caccttggaa acgataagtg ttagaatcaa gtagaaagac gtttttgtaa atttcactaa
     1261 ataattcttt agtgaaaagc atcaaaacga atgaacaaac agataggagt cttacaaatg
     1321 agaatgaatg tcgaaagttt taatcttgac catacaaaag tcaaagcacc ttatgtacgt
     1381 gtagctgatc gaaaaacggg tgcgcatggc gatgtcatca ttaaatacga cgtccgcttt
     1441 aaacaaccaa ataaagaaca catggacatg ccaagtctac attcacttga gcacttgact
     1501 gccgaactaa tccgcaatca cgcagattac atcgtagact ggtctccaat gggatgccaa
     1561 acaggctttt acttgactgt attgaatcat gaaagctacg aagacatttt agatgttctt
     1621 gaagcaacaa tgaaagatgt tcttgaagct actgaagtac ctgcaagcaa tgaaacacaa
     1681 tgtggctggg ctgccagcca tacactagaa ggtgcacaag ctttggctcg tgaattttta
     1741 gcaaaacgcg atgaatggtc tgaagtagaa gcatagtttc taaaagaacg taaagatgag
     1801 aagcaatat
//
LOCUS       DAGCYL010000255         1800 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.267, whole genome shotgun sequence.
ACCESSION   DAGCYL010000255 DAGCYL010000000
VERSION     DAGCYL010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.267"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            109..1722
                     /locus_tag="MB216_002694"
     CDS             109..1722
                     /locus_tag="MB216_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBT4527470.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          569 a          329 c          376 g          526 t
ORIGIN      
        1 ttctctatat tttctgtcta ttcaaaataa acaataattt tccggcacaa ttatttgctg
       61 ttttttacca taacatagta cgatcaacgt agaaaaggag atgtttttat gaaagttgtt
      121 gtcattggcg gtgtagctgg cggtccttca tttgccactc gtttccgtcg attgaatgaa
      181 gcgcacgaaa tcattatcta tgaacgcgga gaaaatattt cttatgcaag ttgtgctttg
      241 ccttattatt taggaggtgt gatcactgac cgtgactcgc tgatcgaacg tacaccagaa
      301 atattgaaaa caaaaaacaa catcgatgtc tttactaaac acgaagtaac agcaatcgat
      361 ccttctacta agcgattaac agttaaagac ctatccacaa atgaagaaat aaaaacagat
      421 tacgataagt tgatcatctc ttctggtgct agaccagatt atccggatat tcccggagtt
      481 tttgaagcag aaaacggctt tgtcctccgt agtgtgacgg atgcggatcg aatcaaatcg
      541 ttccttgaag aaaaaaatcc acaacatgtc gtcattcttg gtgcaggtgt tatgggtctg
      601 gaattagctg agaatttcaa gcatcgcggc ttagacgtga ctttaatcga tcaattgcca
      661 caagtcgctt tcccttatga tccagaaatt gctaatttag tttatgacaa attgctgaaa
      721 gaaggattag ccgttcattt agaaacaaga gttactgaga tccgtgataa aggtcgagaa
      781 atagtattat cagatggctc cgtcctttct gctgatatgc taatttttgc tgttggtgtt
      841 tctccgaaga atgaagtggt gaaagcaacc ggcatacaat tatcagatac aggtcagatc
      901 attgttgatg accagttaca aaccaatctg ccggacatct atgcgattgg cgatattatc
      961 gaaacgacta gtgtagtgac tggtcagccg atccaaagta tgctttccag tgcggccaat
     1021 cgtcaaggac atatgctggc agatatttta aatggtacgc ctatgcgcta tcgcggatat
     1081 attggtgcag gtgtcgcaaa aatctttgat catacagcaa gttatgcggg aatgacagaa
     1141 catgaattaa aagcatcagg cataacaaat tataaaactg tttttatcac tccctttgac
     1201 catgcctatt tctatccaga agctacaaga ttaaatctaa agctgatttt tgatgcagat
     1261 agcggtcgta ttttaggtgg acaagcattt ggagaaaaag gtgtcgataa acggatggga
     1321 gaactttctg tagcaatcac cggaaacttg acagtctttg atttgcctga tttggagttg
     1381 ccttactctc caccgtattc tactacccgt gacccgttga atatagctgg ttatgtcgca
     1441 atcaatcaaa tgacgaatat cgtagaaacg atcaaagcaa gtgatatacc cgaaaacgat
     1501 ttgaaagaag cgttcttttt agatatacgt gaacctaata aagcaccttc tggcagtatc
     1561 tcggcaacaa aaaatattcc aatgaatgag ttacgagatc gaatcaatga aatcccaaaa
     1621 gataaaaaag tttacattac tttcagaaga ggattgaata cttatacttc tgcccgaatc
     1681 ttggcaggtt tgggtatcaa agcgattttg attgaagaat aaaaaaacga ccgcggtggg
     1741 tagccgcggt ctaggagtta tttttacttg aggagtaaaa atgaaaaagt atatagggtt
//
LOCUS       DAGCYL010000256         1786 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.268, whole genome shotgun sequence.
ACCESSION   DAGCYL010000256 DAGCYL010000000
VERSION     DAGCYL010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.268"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            113..1744
                     /locus_tag="MB216_002695"
     CDS             113..1744
                     /locus_tag="MB216_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBT4527471.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT          516 a          313 c          395 g          562 t
ORIGIN      
        1 aataagaatt tatctaacag gaaactttgt gaaaaatatt actttatctc atgaaatatg
       61 atattctaat gaatggtatt ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa
      121 aaattatgtt tcctcactaa aaaaagaatt ttcaggttat gatctttcaa gatttcagaa
      181 agatcttttg gctgggatca cagtagctgc tgtagcattg cctttagcct tggcttttgg
      241 ggtatcaagc ggagctgatg cggccgcagg tttgattacg gctatcattg ccggtatcgt
      301 tattggcggt ttatcaggcg gtttttatca aatatcaggt ccgacaggtg cgatggcagc
      361 tatcttgatg tctattgcgg caacaaaggg aatgcaaggt gtattgcttg ctactttttt
      421 agcgggtcta tttttacttg cagcaggact tttccgatta ggaacactga cttcttttat
      481 cccagcaccc gtaattacgg ggtttacttc aggtattgct atcattattg cattaggtca
      541 gatcgataat ttatttggtg ttcactcgga aggtacaaac gttattgaaa aactaagtag
      601 ttatcaaacg ataggatttc cgataaacac agcctctttg atcatgggag ggctagtcat
      661 tttagggatg tttatctatc caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc
      721 tatcattttg gcaacagcag ttatgctatt gttccattta ccagtagcaa ctgtgggtaa
      781 gattccacaa acattgatca gtagtaatcg tttgaatatg ggagatttta gcttttctgc
      841 cttgcaacaa gtggcagttc cagctattag cattgcttta ttaggaatga ttgaaagctt
      901 gctatgtggt gcttcggctg gccgaatggc aaataagccc ttagatagca atcaagaatt
      961 aatggcgcaa ggaattggaa atctgttgct tcctttcttt ggcgggattc ctgctactgc
     1021 ggccatcgcc cgtacaagcg tggccatcaa atctggtgca caaactagat tagctggtat
     1081 gatacatgca gttgtcttac tgctctccat gcttgttttt gcgccagtga tgtcacagat
     1141 tccaatgcca gcacttgcag gtgttttgat tgtgacggct tggcgtatga atgaatggga
     1201 aacgatcaag gagctattta ctaataaata ttggtcagca gttctattgt ttatccttac
     1261 gatgatttgt acagttatct ttgatttaag tattgctatc gtgatcgggg tgatcggcgg
     1321 ctgtgtattt tttgttgtca aaagcgctgc tatcacgatt tcattagaaa cgatcgattg
     1381 gcaaagaatg gggctgccag aaacggaaaa gctgaataac tggacagtag tgtatattag
     1441 tggtccactg ttttttatgt cttcagaaaa actcaaaaat aaattggaag aactaaatca
     1501 tcacgatggt gtcattttct ccatgcgagg cgtaccaagt attgatttga cggcacgtgc
     1561 agtttttgat gaatttcaag aaaaagcaga gcaaagagga caaacgatta tctatacttc
     1621 cttgcagcca gaagtagaaa aacaactttc tcacttgtgg gaacatacat tcacgcatca
     1681 acatcaaaca gttgctcatg cgttgacagc tttacatgcg aaatatactg caattagcca
     1741 ttaaaacaat gaaaaagaac agacaagact gtaaactgaa gatttt
//
LOCUS       DAGCYL010000257         1777 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.269, whole genome shotgun sequence.
ACCESSION   DAGCYL010000257 DAGCYL010000000
VERSION     DAGCYL010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.269"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            200..427
                     /locus_tag="MB216_002696"
     CDS             200..427
                     /locus_tag="MB216_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBT4527472.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            550..876
                     /locus_tag="MB216_002697"
     CDS             550..876
                     /locus_tag="MB216_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBT4527473.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            944..1657
                     /locus_tag="MB216_002698"
     CDS             944..1657
                     /locus_tag="MB216_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBT4527474.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT          687 a          273 c          374 g          443 t
ORIGIN      
        1 ttaaacaaag tagttgacac ttccgctact tagtattagt acgaaagtat acttcatgat
       61 actaagtaga taagaagtga aaagcggaac ccacgatcgt caaaatgcta actgctttgc
      121 ttacaccaga taaagggaag agcaatatcg cttattttag gcgtggagaa gcaagcaaat
      181 ccctacgatc acaaaaaaaa tgctgaccca gcagttaaga gagttagaac aagatgaaag
      241 tatccatcga aaagcctata accaagtccc gcctaaagta gagtactcgc tcaccgaaga
      301 aggaaaatca ttaagagaga ttctagtagc gttgtctgtt tgggaagaag aacgtatcga
      361 aaaacaaaaa gcctcaaggg aagaagacac gctactgcat acacatggtg gctacctaaa
      421 ttattgattt tctatgacat ttattttaaa taaaagaaaa caggagtttt tttaaaagca
      481 tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaagtg attggaagaa
      541 ggcaagcaaa tgaatagtat ggaaaaagtc aaccagttta gagatgaacg taattggcga
      601 caatttcata acgaaaaaga tttggctatc tctatttctt tggaagcatc tgaattgtta
      661 gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga acgaatcaaa
      721 gaagaactgg cagatgtatt gatctattca tacatgatgg cagacaacct tcacctggat
      781 ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc tgtagaaaaa
      841 agcgcaggtg tacgaaaaaa atacacagag ttgtagaaat gtgtcattcg gctgttggaa
      901 agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa aaaaaggaaa
      961 agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag gactagcttt
     1021 gattttcaat gaacagatca aggattattt tgtcagagag acaggagata aatatgctat
     1081 agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca gttttgactt
     1141 tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcaaatga gaggaacaga
     1201 tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac cggttttcaa
     1261 aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag atcaagaaat
     1321 gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat tgctgtttac
     1381 accgctagaa aatttagaga tgggtgccaa gatctatttg acagatttag aaaatgtgta
     1441 tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat tactgaatga
     1501 agtcgaggga aaagaaattg tcacactaat tacttgtggc gacatggacg ccgtcacgcg
     1561 actagttgta caaggagaac tggaaagtgt tacatcgata aaagatgcga cagatgatat
     1621 gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat aagtcgttac
     1681 agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc tgaaaaaaag
     1741 agaaacgcaa agaggtgcat ctctgatttg gttgttt
//
LOCUS       DAGCYL010000258         1776 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.270, whole genome shotgun sequence.
ACCESSION   DAGCYL010000258 DAGCYL010000000
VERSION     DAGCYL010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.270"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            145..336
                     /locus_tag="MB216_002699"
     CDS             145..336
                     /locus_tag="MB216_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4527475.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            850..1182
                     /locus_tag="MB216_002700"
     CDS             850..1182
                     /locus_tag="MB216_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4527476.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1092..1295
                     /locus_tag="MB216_002701"
     CDS             1092..1295
                     /locus_tag="MB216_002701"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBT4527477.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            1479..1634
                     /locus_tag="MB216_002702"
     CDS             1479..1634
                     /locus_tag="MB216_002702"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBT4527478.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT          650 a          298 c          302 g          526 t
ORIGIN      
        1 atttaaaaca tcgactttac aaaaaaattc aaaaaatgct ggtttaatat ctgcataata
       61 actaattttg tatgcttctt tgctcgtcct cctccttccc aaaaaaataa atagattata
      121 agttcatggg aagaaggtaa atttatgaat actttaaaag aagatttcat tttagcaaag
      181 aatggaaatg aagaagcagt tgtggccatt ctcaaaagat ttagcgcttt ggtccataag
      241 caatcctgga gaactggaaa atatgatcaa gattgttatc aagaatgtat gcttgcaata
      301 tatctagcta tctcgaaatt cgaaattaaa gaataactca actaagggag tctgtgacat
      361 aagtaaaaag atagtttcaa atttcgaaca ataaatttga agccatcttc tttataatta
      421 taaagtatcg attttacagc gttctataat gattcataga atctaaaacg aatgatttta
      481 attattaacg ttttttaatt cgtttttagc ttaaaaataa cttttgtcac agtctcactc
      541 ctaaaattac tttatttacg tacgaagtgg gcaggaggtc gaatccagaa ctgggccatg
      601 gtacttcaac gaggacttct cctctagaat tgaaaagcat gcgatttacc ttccgtaacg
      661 acggtagatt tattcaacta tgtgtccaat caaaatttgt ctgatttcaa aaatagtcaa
      721 gagaattttg gttgttaaaa actcttatct atcaacttcc acagtttagt tgacaaaccc
      781 tgatactaag ttgggctatg atacactttt cttgactatc agttacgtta ccggaaggaa
      841 tgaactctat tgaaaaaaac atatgatcgt aaatttaaac taaagatttc acaggatatc
      901 cttgagaaaa aagtaactac caagaaaatt gctgaagaat acaatatctc tcgtccaaca
      961 atttcccgat gggtttcaga atatcgtcga tacgggaaaa atgcttttgc tggacaagga
     1021 aaaagattac ctgataaagc agatttctac attcttgaac aggaaaataa acgactaaaa
     1081 gaggaagacg agtgttctac gaacacaaag atcgatacgg atctccaggg atcactcaaa
     1141 aactttacga tgaaggaatc gaaatcaata aacgtgttgt agctgttctt atgcgtgaaa
     1201 tgaatttatg cacaagagga tttcatcgac gcaaatcttc tcatggtaga gaaaaaacaa
     1261 tcaaagaaat ggtaaaaaaa atgttgaatc gataatttga acagaaccaa attgatgctg
     1321 tttgggtgac ggatattact tacattccat gtaatgacgg tcgcctctat ttgtccacct
     1381 atattgactt agcaactcgt attctacgtt actatatggt ggattcacac atgaaaaaag
     1441 agattgtcgt ccgtccatta caaatttatt aaggagaatt gcctacggta attcattccg
     1501 atagaggtag ccagcatcgg tcttttattt atcaagagct gttgaccgac caccacatca
     1561 cacattcaat gagccaaact gggactcctg ttgacaatgc agtaattgaa gcgtttcatc
     1621 gatcaatcaa atgagaatta atcgactcca acaaatataa aagtaaaata gagatgaagg
     1681 tgttaatcca agattattta gaagactact acccaaacaa aagaatccat acaaaattta
     1741 tgatgactcc ttgacaattt gaagcgaaga aaataa
//
LOCUS       DAGCYL010000259         1705 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.272, whole genome shotgun sequence.
ACCESSION   DAGCYL010000259 DAGCYL010000000
VERSION     DAGCYL010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.272"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1352
                     /locus_tag="MB216_002703"
     CDS             <1..1352
                     /locus_tag="MB216_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBT4527479.1"
                     /translation="DEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLL
                     PLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKK
                     AIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIK
                     AVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYY
                     HHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKK
                     NTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAK
                     SKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGART
                     SGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
     gene            complement(1533..>1705)
                     /locus_tag="MB216_002704"
     CDS             complement(1533..>1705)
                     /locus_tag="MB216_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBT4527480.1"
                     /translation="NKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQ
                     QVTDTLVSMFTE"
BASE COUNT          557 a          300 c          350 g          498 t
ORIGIN      
        1 gtgatgaaga aatttctgta agtgttccag aagcagctgc caccattatt ccagtaaaag
       61 gacatcaacg agttgtagga acgaaagcag acaaaatcaa acacttacct ataacagaga
      121 aagaacatct ccttcctttg gaagaacaat tttgtgaaca ttgcggttca cagatgaaag
      181 atatcggacg gacaaaagta cgagaagaaa ttcgtttcca tcaggctatg ttggattgtc
      241 tcacccatta tcaacatacc tattgttgta aaagctgcga aaaagaagga ctttcttctt
      301 ttaaaaaagc gatcgttcct aaacctttga tttctaatag cttaggttcc aatagtttag
      361 tggcggaaac gatccggatg aaatttggtc agaaagttcc tgcctatcgg caagagaact
      421 attggaaaca aacacatggg ttggatattt cgagagataa tatcaccaat tggcatatta
      481 aagcagtgca aaacgcgtta gaccctttag gagaacgatt aagagtctat cttaatcaag
      541 aagagattct acacggggat gaaacaagtt atcgtgtgat tgaaagtgcc aaaacagata
      601 cctattactg gcagttttgt acaggaaaag acagtcagca tcccatcgtt tattatcacc
      661 acgacgaaag tcgtgctgga gatgttccga aaacttttct caaggagttt acagggtatt
      721 tacactgtga cggctatagc gggtataatg ccgtggaaag tgtacgactt gtctattgtt
      781 tcgcgcatgt tcggcggaaa tttttcgaag caattccaaa aggaaagaaa aacacggata
      841 ttcccgcagc tcaagcagtc aaacaattgg ataaatggtt tgtcctagaa aagaaatgga
      901 aagacttttc tcctgagaag cgattaagct gtcggcaaca agaactgcgt ccattattta
      961 ttgcttttta tgagtggatg gcaacgatag atcctgttgc aaaatccaag ttggatgcag
     1021 ccgttcaata tgcgtgtaaa ctacgaagtg gttttgaacc tatttttgaa gatggtcgtt
     1081 tagaactcac aaataatcgt gcggagcgaa atatcaaaga gctggtgata ggacggaaaa
     1141 attggttgca ttccacaagt cttgaaggag cacgaacttc aggaatcatt ctgagtgtat
     1201 ataaaacagc agaacttaac ggattgaatc ctgtaaagta tctagagttt ctatttgata
     1261 agataccaaa tcttcccgta ctttcagcag aaactttaga tcaactgttg ccttggaaca
     1321 aggatgtaca acaacatttt tctcgtaact aaaaacgcca aacattcccg attctattct
     1381 ataggtcggg ttatttgacg tacaggtact gttctatggg gcgcttacga actttataaa
     1441 attcaattat gacataaaag ccaaacttaa tgatcagttt gggagcgtca ataattttgt
     1501 gtaaataaat tgtcctcctg caaaataatt agttactcag taaacattga aactaatgta
     1561 tcggttacct gttgaaaacc tttatggctt ctgtttagaa atttttgatt gtatgtatca
     1621 aaaatgctga ctagaaagcg ttctagtgat tcttcatttt gaaactgctc ttttctacgg
     1681 ctgtatcttt taatttgctt attga
//
LOCUS       DAGCYL010000260         1679 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.273, whole genome shotgun sequence.
ACCESSION   DAGCYL010000260 DAGCYL010000000
VERSION     DAGCYL010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.273"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            183..1661
                     /locus_tag="MB216_002705"
     CDS             183..1661
                     /locus_tag="MB216_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4527481.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          618 a          231 c          299 g          531 t
ORIGIN      
        1 aatatagaat atttcctcat aaatttttcc attttttgca atattctcaa aaaaaagata
       61 aaaaatgaga ataactgatg aaaaaacaag cataatccat cataaaatca gtataggtga
      121 aaagatgaac tttcatctgc atatcaagta attggtatga ggcatttttt gaggtgtagg
      181 atatgaagtt ggattacctt atagaagagg aagatagaga aaaactgctg ctagctagat
      241 atttagaatt tgagaaaaag aattatgtag tgctgcctaa gttatatgaa acattaggtt
      301 tgtctaaatt taaaacaaga aattatatca tagaattaaa taaagaattt actgaattca
      361 aaacaaagcc agagatccaa atttcagaag aagcagagat ccatgtcgaa cgtctgaatt
      421 tgacggatat caaaaattta agaagaatct actttcaacg atcaaaaatt gctcgactgt
      481 taacggagat cgtggattca aaagtaacga tcgaaaaatt tgcagagaat cattttttaa
      541 gcatatcaag agcctatgta aaaagaagag aattagtctc ttttcttgaa cagcatcaaa
      601 taaagttaaa gaaaaacata ttagttgggg aagaaataaa catacggaac gtattatttg
      661 gggtatattt cggggtcttt aatggttttt ctatgccatt tagagaaagc gtcatgaatg
      721 aagcaaagag tatcgtgcaa tattttgaat atctgttcaa tttaaaactg tctgcaacaa
      781 aacgaatcaa attcaatttg ctaaccacta ttagtttgtt gagggcctat gggaatcaac
      841 caatcggaaa acgtttcttt tcagatgaat actttaagag ctcagaagga atgaagatca
      901 tcaaggattt atcacatatt tcttttcttc atatccctaa agagcagatc attgatgaga
      961 tttcttatat catgttgttt ttgatcacgg aagaagaatt tgaattagat ggcgcatttg
     1021 gacttgcgga agctcatttt catgatatcc acatgattag tcaaaaaatg tcagtggaat
     1081 tgattgatca attaccattt tcgattgaaa tctctgaaga agaaagacta tatatacgaa
     1141 aagaacttcg tatgggtatg atgcatgtca atcgaaaaaa ttggatattc gactttgaga
     1201 tgactagctt tacgtctaaa aagcagctac agtttttctt cgagacgtat ccaatattca
     1261 gtacagcaat ccaaaaggtc atacaagaga ataaacgcta tttcttggga aaagatgaac
     1321 gtttcatgat tcgcttattt tatgattatc tctttttatt ggtcggtatt tgtccggtat
     1381 catattttga agcaccgatt catgtctgta ttgatttttc gcaaggatct gcttataccg
     1441 attacattat ttctcaagtg aaaggattta aaaattacaa tattgtgatt gagagtcgaa
     1501 tcacttctcg tacgaatatt tatttatctg attgtttact tgaaaaattt aataaagaac
     1561 agataatttg gaaaaatcca ccgactccag atgactggga atattttgga aataccatca
     1621 tcagagaaaa aaatagacat ttaactgaaa aaatcagcta gtatttatgg atggcagct
//
LOCUS       DAGCYL010000261         1649 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.274, whole genome shotgun sequence.
ACCESSION   DAGCYL010000261 DAGCYL010000000
VERSION     DAGCYL010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.274"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..847
                     /locus_tag="MB216_002706"
     CDS             <1..847
                     /locus_tag="MB216_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBT4527482.1"
                     /translation="IKPDMVIAYTIKPNIYGGLACRLRKVSYALNITGLGTAFQKKGV
                     LKSLIVFLYRLAAKKSKVIFFENFENMKTFQTLQIAKQSKYKLLNGAGVDVEHFSYLE
                     YPKDEQVHFLFIGRIMKEKGVDELFEAMKNLYSEEKNCVLDVLGYFEEDYGIKIEKYE
                     KEGWLKYHGYQSDIRPFVERAHCFVLPSWHEGMANTNLESAASGRPIITSDIPGCKEA
                     VIESKSGYLCERKNSCSLYNSMNKFLKLSYEQRINMGKMGRNYMIKKFNKKKVVDETI
                     KSLFS"
     gene            893..>1649
                     /locus_tag="MB216_002707"
     CDS             893..>1649
                     /locus_tag="MB216_002707"
                     /inference="COORDINATES: protein motif:HMM:NF025025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4527483.1"
                     /translation="MSNVSNPLVSVIMGAYNSEKTIGRAIDSIIKQTYLHWEMIICDD
                     GSTDGTVKIIRKYVEKDKRIKLVQNKKNSGLAYSLNHCLKYSSGKYVARMDADDISLP
                     ERFAYQVGFFEKNPEYDVVGCNRIVFDENGDSGIRKSKEIPTINDMYKGSPFAHPTIM
                     MKRSVYTILGGYRVNKDTTRSEDLEMWFRFFNEGFRGYNLQRVLFKYQETSEDFHKRS
                     LRAAYGITKVLLRGYKLIGVSKYKYIYAFKPIIS"
BASE COUNT          646 a          178 c          324 g          501 t
ORIGIN      
        1 aataaagcca gatatggtca ttgcttatac tatcaaacca aatatatacg gtggattggc
       61 atgtcggtta agaaaggttt cttatgcatt aaatataacc ggattaggaa cggcatttca
      121 aaaaaagggg gttttgaaaa gtcttattgt atttttgtat agattagcag ctaaaaaatc
      181 taaggtcata tttttcgaaa attttgaaaa tatgaaaaca tttcagactt tacaaattgc
      241 taaacaaagc aaatacaagc tattaaacgg tgcaggagta gatgtagaac actttagtta
      301 tttagaatat cctaaagatg agcaagttca ttttttattt attggaagaa ttatgaaaga
      361 gaaaggtgta gatgaacttt ttgaagcaat gaaaaactta tatagtgaag agaaaaattg
      421 tgtacttgat gtccttgggt attttgaaga agattacggc ataaaaattg aaaaatacga
      481 aaaagaggga tggttaaaat atcatggata tcaatctgat attcgtccct ttgtagaaag
      541 agcacattgc tttgttttgc ctagttggca cgaaggaatg gcaaatacta accttgaatc
      601 agcagcatca gggagaccaa ttattacaag tgatatccca gggtgtaaag aagcagttat
      661 tgaatcgaag tctggttatt tatgtgaaag aaaaaatagt tgtagtctct ataattcgat
      721 gaataaattt ctaaaattat catatgagca gcgcattaac atgggaaaaa tggggagaaa
      781 ttatatgatt aaaaaattta ataagaagaa agttgtcgat gaaacaatta aatcattatt
      841 tagttaaaaa gatatgtttt gcaaagtatg tgatagaaaa gagggattct gtttgagtaa
      901 cgtaagtaat ccattagtaa gtgtaatcat gggagcctac aattcagaga aaacaattgg
      961 aagagcaatt gattcaatta taaaacaaac atatttacat tgggaaatga taatttgcga
     1021 tgatggatca actgatggaa ctgtaaaaat aatacggaaa tatgtagaaa aggataaacg
     1081 tattaagctt gttcaaaata aaaaaaatag tggattagca tattcattaa atcattgttt
     1141 gaaatattca tcgggaaaat atgttgcaag gatggatgca gatgatatta gtttaccaga
     1201 aagatttgca taccaagttg gattctttga aaaaaatcca gaatatgacg ttgtaggatg
     1261 taatcgaata gtattcgatg aaaatggtga ttctggaata cgaaaatcaa aggaaattcc
     1321 aactattaat gacatgtata aaggttctcc atttgctcat ccgacaataa tgatgaaacg
     1381 aagtgtttat actattctag gtggttacag ggttaacaaa gatacaacaa gatcagaaga
     1441 tttagaaatg tggtttcgat tcttcaatga aggatttaga ggttataatt tacaacgagt
     1501 cttatttaaa tatcaagaaa caagcgaaga ttttcataaa agaagtttaa gagcagctta
     1561 cggaataaca aaagtattgt tgagaggtta taagttaata ggagttagta aatataaata
     1621 tatttacgca tttaagccga ttatttccg
//
LOCUS       DAGCYL010000262         1608 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.275, whole genome shotgun sequence.
ACCESSION   DAGCYL010000262 DAGCYL010000000
VERSION     DAGCYL010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.275"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            28..987
                     /locus_tag="MB216_002708"
     CDS             28..987
                     /locus_tag="MB216_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBT4527484.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1120..1326
                     /locus_tag="MB216_002709"
     CDS             1120..1326
                     /locus_tag="MB216_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBT4527485.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            1337..>1608
                     /locus_tag="MB216_002710"
     CDS             1337..>1608
                     /locus_tag="MB216_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBT4527486.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVG"
BASE COUNT          556 a          257 c          325 g          470 t
ORIGIN      
        1 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
       61 gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg
      121 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      181 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      241 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      301 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
      361 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
      421 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
      481 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
      541 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
      601 aaaccgtgtg gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct
      661 gtaccgaaaa acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg
      721 aaacagatca gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct
      781 caaagaggcc taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct
      841 atggatttca attcagtaga tgaatttttt attcaatctg tcgcatctaa acgaaataat
      901 attcctagaa aatcactgga ttatcgaaca cctttggaag tatttttgag ttacgtaagt
      961 attgatgatc tgtctaactt aatttgacaa ttaagaaaga taaaattgaa caactggacg
     1021 ggcaaattga tgccttgctt tcagaaagag aaatattgat agaagaaaac aagcgattga
     1081 aaaagaaagt agcccaacca atgaagggag gtggatagta tgaacaatag aacctttgat
     1141 attttgaaat ggatcgctat agtagttatc cccgctttag cgacttttgt cggtcttgtc
     1201 ggaaaaggtg ttaactggcg gtacacagat ataacggtga tgattattac aggttttgga
     1261 gcatttctag ggagtatttt aggtgtatca aatcgaacgt ataagaaaat ttcatcagat
     1321 aaataggagg atagatatga aaaagaaaat tactattact gcgatgagcc tgttaacggc
     1381 tcttttttta ttgccaatta acggatttgc ctatactatc aacaatgaat ttaatttggg
     1441 cgcaaatgaa ggtagctcac aagtagcaaa taatcagtat attttactgc atgaaacggc
     1501 taatgaaaca gcaacaggac gcaatgaagc gcagtatatg caacgttcat ggactagcgc
     1561 ttatactgct tatattgtgg gagacggcgg aattgtttat caagtcgg
//
LOCUS       DAGCYL010000263         1598 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.276, whole genome shotgun sequence.
ACCESSION   DAGCYL010000263 DAGCYL010000000
VERSION     DAGCYL010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.276"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            121..>1598
                     /locus_tag="MB216_002711"
     CDS             121..>1598
                     /locus_tag="MB216_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBT4527487.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDIPQNMMSAIVDSNRTRKDFIADRVLQKTGYYSYDDDN
                     TFDLSLEKEVIIGVYRLTMKSNSDNFRQSSIQGIMKRIKAKGATVIIYEPTLKDGSTF
                     FGSKVINDLEKFKSISHSIIANRYDSILNDVKEKVYTRDLFQR"
BASE COUNT          610 a          268 c          254 g          466 t
ORIGIN      
        1 taaaatcaaa aaatatcttt ttaatcaata aaaaaattga cagttttatt aataataagc
       61 tattctgtta tagaagaaaa aattgtttgt tattattagc aacaaaaaaa ggagtgcatc
      121 atgaaaaagt tatctcaaaa acgtttagct caaatcatta ttgattctag aaaatctctg
      181 aaaatgacac aacaacaact tgcagtagct actggtatca accgtgcctt aattagtcga
      241 gtagaatctc tcgattttat cccgtcaata caacaattag aatcattagc agaagttcta
      301 aattttgaac caacagatct atttataaat gaatcaaata aatcaaacag aacaaaacat
      361 cagtcgaaga atattgctgt agccggtact ggttacgtag gtctttccat tgctactctt
      421 cttgcccagc acaatcaggt aacagcagta gacattcttc ctgaaaaagt aaaacaaatt
      481 aacaataaga aatctccaat acaagacgat tacattgaaa aatatctagc tgaaaaaaat
      541 cttaatttaa ttgcaacgac tgatggtgca gccgcctata gagaagccga ttttgttgtc
      601 attgctgctc caacaaatta tgatagtaaa caaaattatt ttgatacttc tgctgtagag
      661 tcagtaatca aattggtgtt agaagtcaat ccaaaggcaa taatggtcat caagtcgacg
      721 gtacccgtag gctataccga aagtattcgt aaaaaataca atactaaaaa tattctattc
      781 agcccagaat ttctcagaga atctaaggca ttatatgaca atctatatcc ttcgcgaatt
      841 attgtcggca cggaccaaac tgacaaaact ttacgagaag cctctcacac ttttgcagat
      901 ttactccaag aaggagcaat caaaaaagat atcgaaactc ttttcatggg attcacagaa
      961 gctgaagcag ttaaattatt cgctaatact taccttgcac ttcgagtatc ttacttcaat
     1021 gagctggata cttatgcaga aatgaaagga ctaaatacac aagaaatcat tcacggggtt
     1081 tgcttagatc caagaattgg cacacattat aataatccat cttttggata tggaggctat
     1141 tgtttaccga aagatacaaa acaacttcta actaactata acgatatacc tcaaaatatg
     1201 atgagtgcaa ttgtcgatag taaccgaaca agaaaagact ttatagcaga ccgcgtgctt
     1261 caaaaaactg gatattattc ttacgatgat gacaatactt tcgatttgtc tctagaaaaa
     1321 gaagtgatta ttggagtcta taggcttaca atgaaaagta attcggataa cttccgtcaa
     1381 tcttctattc aaggtattat gaaaagaatc aaggcaaagg gagccactgt gataatttac
     1441 gagccaacac ttaaagatgg tagtacattt tttggttcaa aagtaataaa tgatttagaa
     1501 aaatttaaaa gcataagtca tagtattatt gctaatcgat atgatagtat tcttaatgat
     1561 gtaaaagaaa aagtatacac tagagattta tttcaacg
//
LOCUS       DAGCYL010000264         1578 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.277, whole genome shotgun sequence.
ACCESSION   DAGCYL010000264 DAGCYL010000000
VERSION     DAGCYL010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.277"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(186..1001)
                     /gene="thiD"
                     /locus_tag="MB216_002712"
     CDS             complement(186..1001)
                     /gene="thiD"
                     /locus_tag="MB216_002712"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBT4527488.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1295..1570
                     /locus_tag="MB216_002713"
     CDS             1295..1570
                     /locus_tag="MB216_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527489.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT          480 a          316 c          248 g          534 t
ORIGIN      
        1 tgttttttga acttgttcct tgtgactcgg agctggacgg caggctcacg acctctttct
       61 taaacggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag
      121 aagctgtttt tacttggctg ttcttattac ttgatgcagg gcaacttttt cacaacttct
      181 ttcttttact ctaattcacc agctgcccaa tgattgatgg ggccatattt gtgccctaca
      241 tttatttctt cacgaattgc acggtttaca tagtcttttg ccaatcggat cgcttgttca
      301 actgtttttc ctttcgctaa ctcagctgca atacatgcag atagcgaatc gccagttccg
      361 ttgatccggt cggtaggata aaatggtgca tttaaccaaa atgattttcc tgattcgaac
      421 aatacgtagt cattgactgt ttcttgctgc gcatttttag catgatgtcc tttgatcata
      481 acgttttttg cacccatatc ttgcagttct tttgctgctt gcacaaaatc ttcttcagct
      541 gctaatgtcc gtccagtcag cttttctgct tcataaaaat taggtgtcag tacttcggcc
      601 aaagggacaa gcttttcttt caagctttgc aaagcagatt cttctagtaa aagatttcca
      661 tgtttcgtta cgattacggg atccacgact aatggtccaa aatcatattt ttggtaattt
      721 tttacaactg tctcaatcaa ctttgaatct gctagcatac ctgttttagc tgcacgtatt
      781 tgaaagtcct tcgcaatcag ttcaaattct ttgtcgataa aatctgttgg caatgtcaca
      841 cttgctccga ttccgtaaga attacctgca acacatgcgg tcatgacaga aacaccgtaa
      901 acatttcttg taaagaatgt atgcatatct gcttgcatcc ctgcgcttcc atcgctatct
      961 gatcctgcga tcgtcattgc ttgtacatac tgatcttcca tcaaatcgat tcctccattt
     1021 ttataaaaag atttttcata atactgttac tgtttctatc atatgttttt caaagtatcc
     1081 ctcagtaaat ataccactac cttttcttgg taatcaaatt tatttgaaaa aacatttgca
     1141 ataaatcaga aaatagtggt cgtttatttg tattcgctat tctaataata aataaccact
     1201 atttgattta tatatatttc agtatccttc ttgttttcat ataaccaaag gcgctatgat
     1261 ttccatatga aaaaaataaa gtcaggtgaa aaaaatgaaa cttgaggata aaaaagtcat
     1321 tttgaagaaa atcaaacgac taccagacca aatagatggc tatacgatct gttctttcaa
     1381 aaaagacagg agtataaaag cttatacgca aaacacagaa aaaggaataa tctatttcct
     1441 acatgaagaa ggttacgata cccaaacctt tatgtttact gaaaaaaaag aatttcataa
     1501 acagatgaaa aaactgattg aaaaagaatt tccaagaagt cataaactct atgtgaatca
     1561 gcaattttaa cttctaac
//
LOCUS       DAGCYL010000265         1531 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.278, whole genome shotgun sequence.
ACCESSION   DAGCYL010000265 DAGCYL010000000
VERSION     DAGCYL010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.278"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            17..1483
                     /locus_tag="MB216_002714"
     CDS             17..1483
                     /locus_tag="MB216_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBT4527490.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          419 a          289 c          325 g          498 t
ORIGIN      
        1 aattggggta tagattatgg aaaaacagca agacaagggc ttcttcgggc agccgaaggg
       61 attgtcaact ttgttcttca ctgagatgtg ggaacgattt agttattacg gtatgcgtgc
      121 attacttatc tattatatgt acgatacagt tgccaacggg ggacttggat taccacgagc
      181 aacagcatta gctatcatgt cgatctacgg atctttgatc tacatgtcta gtatcgtcgg
      241 cggatgggta tctgaccgag tgttaggagc aaaacgcact gtattcttcg gtggagtttt
      301 cattatgctt ggccacatcg tcttggcttt accatttggt gtaagtacat tgtttgtttc
      361 gatggcattg attattatcg gaaccgcctt tttgaaacca aatgtttctg gcatggtcgg
      421 acatctttat tcaaagactg acttgcgaag agatgcagga ttttcgattt tttacatggg
      481 tgtcaactta ggggcattat tagcaccact aatcgttggt acaatcggtc aagaatataa
      541 ctatcattta gggttctctt tagctgctat tgggatgttc tttggattat tacaatacat
      601 catccaagga cgcaagacat tagacggagt gggtacaaca ccgccgaatc ctttgacaac
      661 tgaagaacgt aaaggattca tccgtaactt gatcatcgca cttgttgtta tcgctgcagt
      721 attcggcggg gcacaactaa ctggtcatct gacaattgac ttttttgtca atacaatcag
      781 tgtattgggg attatcttac cgttgtatta ctttatcaaa atgctgactt caaaagatgt
      841 aactgctgaa gaaaaaccaa gagtatgggc ttatatccca ttattccttg cagcaatcat
      901 tttctggtca ttacaagaac aaggttcatc tatcttagct ttatttgcta gtgaacgtac
      961 acaatctacg ctatttggtg taacgatcca agcaagctgg tatcaatcat tgaatccaat
     1021 tttcatcttt attctgacac cagtattcgt aacgatgtgg acgaaacttg gtgaacgtca
     1081 accatctaca gtagtgaaat tctctcttgg acttttattt gcaggattat cgtacttgct
     1141 attgatgttg ccgggtatgc tttatggtac aagtggaaaa gtaagtccat tgtggttgtt
     1201 gggaagtttc ttcatcgtgg ttattgctga attgtgtctt tcaccagttg gtttgtctgt
     1261 gacaacaaaa ttagcaccac gagcatttga atcacaaaca atcgctattt ggttcttagc
     1321 tgatgcttct tcacaagcaa tcaatgcaca aattgcacgc ttctacacac cgggaacaga
     1381 atctgcttac ttcggtatta tcggtgcagt agcaatcgtc ggtggtatct tgctatttac
     1441 tgtgaaagaa ccaatcaaaa aactaatggg ctctattcat taatcaatga acataaaaac
     1501 aggaattgct tatttgcagc agttcctgtt t
//
LOCUS       DAGCYL010000266         1531 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.279, whole genome shotgun sequence.
ACCESSION   DAGCYL010000266 DAGCYL010000000
VERSION     DAGCYL010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.279"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1342
                     /locus_tag="MB216_002715"
     rRNA            <1..1342
                     /locus_tag="MB216_002715"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1437..>1531
                     /gene="rrf"
                     /locus_tag="MB216_002716"
     rRNA            1437..>1531
                     /gene="rrf"
                     /locus_tag="MB216_002716"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          428 a          329 c          449 g          325 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaaagaa ctcggaaaaa aacttatgtg
     1381 acttcaaatc cagttttgag tgaacaaaat ttactcaata aaagataaca ccacagtgtg
     1441 gtggcgatag cgagaaggat acacctgtaa ccatgccgaa cacagaagtt aagcttctta
     1501 gcgccgattg tagtgagggg ttgccccttg t
//
LOCUS       DAGCYL010000267         1515 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.280, whole genome shotgun sequence.
ACCESSION   DAGCYL010000267 DAGCYL010000000
VERSION     DAGCYL010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.280"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            182..1477
                     /locus_tag="MB216_002717"
     CDS             182..1477
                     /locus_tag="MB216_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4527491.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          473 a          297 c          284 g          461 t
ORIGIN      
        1 gctgtttttt acatagacac aaaaaagaaa ccacgcttgt agacgtggtt tcttttttag
       61 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
      121 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
      181 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      241 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      301 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      361 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      421 ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc
      481 ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat
      541 tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac
      601 ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac
      661 tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat
      721 gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg
      781 cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca
      841 attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa
      901 tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa
      961 tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt
     1021 agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta
     1081 tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga
     1141 tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct
     1201 ttatgcggta aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag
     1261 cggtctgcca gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat
     1321 ccaaaatgcg cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat
     1381 caaagtatta aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aactacgaat
     1441 tatgattcgt catggcaaaa catttttaac caaataaaca gaacagaaag aggttttctt
     1501 ctttctgttc tgtcc
//
LOCUS       DAGCYL010000268         1501 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.281, whole genome shotgun sequence.
ACCESSION   DAGCYL010000268 DAGCYL010000000
VERSION     DAGCYL010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.281"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            5..403
                     /locus_tag="MB216_002718"
     CDS             5..403
                     /locus_tag="MB216_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBT4527492.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="MB216_002719"
     CDS             387..1082
                     /locus_tag="MB216_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBT4527493.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          568 a          216 c          302 g          415 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 a
//
LOCUS       DAGCYL010000269         1501 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.282, whole genome shotgun sequence.
ACCESSION   DAGCYL010000269 DAGCYL010000000
VERSION     DAGCYL010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.282"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..384
                     /locus_tag="MB216_002720"
     CDS             <1..384
                     /locus_tag="MB216_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527494.1"
                     /translation="IGISVTGTTIPTFADSTNEPTTVVDNNQELTPLDRNGIAIGNPT
                     NDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGYWYYG
                     KLYLQSYKSAGNNWIATYSGTLTRK"
     gene            394..597
                     /locus_tag="MB216_002721"
     CDS             394..597
                     /locus_tag="MB216_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527495.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            808..1392
                     /locus_tag="MB216_002722"
     CDS             808..1392
                     /locus_tag="MB216_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4527496.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          533 a          220 c          283 g          465 t
ORIGIN      
        1 attggaatta gtgtaactgg aactacaatt cctacatttg ctgatagtac aaatgaacca
       61 actactgtag tagataataa ccaagaatta actcctttag ataggaacgg aatagccatt
      121 ggtaacccaa ctaacgactt aactgttact gaaaattatt taatagtttc tccattactt
      181 gctcgagcag cttctaaaac gatacgagtg acaggaacga tgatttttga taaagttggt
      241 tggccttcta gacctcctca atcacggaat tggaaagaaa aaagaaatgg ttattggtac
      301 tatggaaaat tgtatttaca aagttataaa tcagctggaa acaattggat tgcgacttat
      361 tcaggaacct taactagaaa gtagggattt tttatgccag aatttgtgaa cacaccaaat
      421 ttttatcaag atagcgatat tctaagtaaa attaatttac cgaaagaaga atcagcttta
      481 caagttcagc gaactttaca ggagacacaa aaaattcaaa aatcaatttc taatctagaa
      541 gattctgaag aaaaaagaaa aaaaagagac caaaaaacat atcaaaaaga aaaataatga
      601 ttttaaaaaa agagtgtcta gtcgacattc tttttttcta attgaaagtg ttcataaaat
      661 cacccatttc tggacacttc ctttttttgc tttatttttc aaaaaaacaa ttcatttcat
      721 gttcataaac ccataacaat atctatgttc agttaaacta tatggataaa gttatgttac
      781 aataaaatta atgaaggagt gatttttttg aaaataggat acgcccgggt atctactggt
      841 ttacaaaatt tggatttaca agaagacagc ctaaataaat ttggttgtga aaaaattttt
      901 actgatcata tgagtggggc aaaaagtaat cgaccaggat tagaaatggc cattgatttt
      961 gttcgctctg gcgatacact tgtcgtttgg cgcttagatc gtttaggtag aaatatggaa
     1021 gatttgattt caatcgtcaa tcgactgaat gagcgtggtg tgagttttca tagtattcaa
     1081 gaaaatatta cgatggacaa atcaagttct acaggacagc tcatgtttca tttgtttgcg
     1141 gcatttgcag aatttgagcg taatttgatc ctggaacgat ccgcagcagg acgagcagct
     1201 gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat tgtctgaaca agatttggaa
     1261 ttactgaaga ctttagtgga tagcggtaca cctattaaga ctattgcgga acggtggaac
     1321 gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg aggagaaaaa tggaaagaca
     1381 aattctcatt agtttttgtc atttaatgct gaaaattttt gccatttatc tagaaaagtg
     1441 acaatagtat gagaattttt tgtaagtgtc tactgataac ttattatcaa atgttgtaag
     1501 g
//
LOCUS       DAGCYL010000270         1478 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.283, whole genome shotgun sequence.
ACCESSION   DAGCYL010000270 DAGCYL010000000
VERSION     DAGCYL010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.283"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            133..507
                     /locus_tag="MB216_002723"
     CDS             133..507
                     /locus_tag="MB216_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBT4527497.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="MB216_002724"
     CDS             complement(621..1250)
                     /locus_tag="MB216_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBT4527498.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="MB216_002725"
     tRNA            complement(1384..1456)
                     /locus_tag="MB216_002725"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
BASE COUNT          499 a          225 c          259 g          495 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataata
//
LOCUS       DAGCYL010000271         1457 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.284, whole genome shotgun sequence.
ACCESSION   DAGCYL010000271 DAGCYL010000000
VERSION     DAGCYL010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.284"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            198..812
                     /locus_tag="MB216_002726"
     CDS             198..812
                     /locus_tag="MB216_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4527499.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(836..1186)
                     /locus_tag="MB216_002727"
     CDS             complement(836..1186)
                     /locus_tag="MB216_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBT4527500.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1180..1443)
                     /locus_tag="MB216_002728"
     CDS             complement(1180..1443)
                     /locus_tag="MB216_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBT4527501.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          463 a          272 c          233 g          489 t
ORIGIN      
        1 aattttcttg attgacattt atttacaaaa aatattgcca tttaaaaaga aaagtgacac
       61 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac
      121 cgcgacattg aaaaatctgg ttttctcgtg tatgattagg gtgtttttta aatagttgca
      181 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
      241 aacaacggca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc attgagaaac
      301 aatctggcgc aacgattact catcgacctg tttttcaaga agcactggat ttcgtccgag
      361 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
      421 tcgcttcagt caattacttg aagaagaaaa atgtgcagct gatcatcaca agtctaccga
      481 tcatggccga agcaattggc aatcctctcc tggataaatt cattaaagat ttgatcattc
      541 aaattttggc catgatcgct gaacaagaac gtaccgaatc taaacgacgt caggcacaag
      601 gcattaagat tgcaaaagcg aacggtgttt ataaaggacg tcctaagcta tacagtgcca
      661 atgcaaaaga tccccaacgt cgtcttgttt ataaaaacat cgttgaggat ctaaaaaaag
      721 gtgttgcgat cgctaagatt gcaaaagact acaatgttac gagacaaacc gtatatagaa
      781 taaaaaaaga cagcatggtc aatcacgaat agttgatcat gctgctgttt ctattttatt
      841 tgcttacttt ttgaaaaact gttctaactt tcatcaataa ctcctcaact aaactatcat
      901 gcgctttttc caaaaaaata cactctctgg cttcataatc aattgtcgtt agttgatcta
      961 ataatacttt tcctgttgtc ttcatttcat attctgagtt tatatttaca tatagcggat
     1021 agtttctagt tgtactagaa attggagcaa ctgtaacaac gttactataa tccgcaataa
     1081 tatcatgact taaaacaata taaggtcttt tacctgtctg ttcgtgtcca gatcgaggtg
     1141 ctgtattcaa taatagaata tcgccttgtc taggttttct caccattttt cattacctac
     1201 tgcttcacct aaattttgaa tcgtcacatg atttctttca tcagtgtaat tttcaaataa
     1261 ctgttcaatt gttaattcat tttctatatt agccgcttcg ataattagcc gcttctgacc
     1321 gtttacttct tcttgtatta tcttcacttt gtcttctgta tgaattccaa gatattccat
     1381 aatatacttt ggcaatctaa ggccattact atttccccat ttttttatag gtaacatttc
     1441 catagtagac acctctt
//
LOCUS       DAGCYL010000272         1438 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.285, whole genome shotgun sequence.
ACCESSION   DAGCYL010000272 DAGCYL010000000
VERSION     DAGCYL010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.285"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            63..614
                     /gene="lepB"
                     /locus_tag="MB216_002729"
     CDS             63..614
                     /gene="lepB"
                     /locus_tag="MB216_002729"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBT4527502.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(697..1197)
                     /locus_tag="MB216_002730"
     CDS             complement(697..1197)
                     /locus_tag="MB216_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBT4527503.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
BASE COUNT          528 a          237 c          265 g          408 t
ORIGIN      
        1 ttttttgata tttttcttat aataaagaaa gaatgaaaaa aagagggatg gattgataga
       61 gattgacaaa aaaacaacgc tatattaatc gcttctggct tgtattcaaa tacttacttg
      121 tttctgtatt ccttgcgttc atgttgcggg gatttttatt catcccggta ccagtggaag
      181 gcaattcaat ggaaaacgtg ttgaagcaag gtgatatggt tgtgatggaa aaattttcgg
      241 agatcagacg atttgatatt gttgtttttc aattagcaga tggcacgatt tatatcaaac
      301 gagtaatcgg tcttccagga gaaaatgtca gctaccaaaa tgatcaattg aagatcaatg
      361 ggaaagtagt taaggaacct tatctaacta aaaacctgaa atcagatcat gcaaatgcat
      421 cctatacgac ggactttaca ctacaagagc taacgggaca atccaagctt ccggaagata
      481 attattttgt acttggagat aatcgaagag tttcaaaaga tagtcgttcg tttggcacga
      541 tcaataaaac tgatattcta ggtaaagcaa gatttgtcta ttatccactt gatgaaataa
      601 aatggatcca gtgagaaaca ataaagcgtg cagtctgttg agactgcacg ctttattgtt
      661 taatccaaaa tcagtttact accttagtac gtaaaatcaa acagctcgtt taaacacttt
      721 tctagcctct aaagttttga taaatgaata aacaaccatt atttgaatag ggatttggat
      781 catattcctg atgaatctac ctagaaaaat tactttgaaa tctgcaccaa acaaaacaga
      841 catcagccaa gaattgataa aaaggctgac tgggatcata atcaataatt gggcagcgat
      901 gacggaaggc aggtttatct ttttatgata taaaaataag ccattaataa atccaccaag
      961 gaaagccgtg ataagaaaag cagcttgaaa cgttcctgaa ccaaatatcg tgaaatttaa
     1021 agtgttcgac aacatttcaa gcaaaccagc ccagatcggt ccatatagat aacccataag
     1081 cgccatactt aaaaatccga atgtgatttg aaaataagca ggaccaatcg ctatcttgta
     1141 taagatgaca tcaagggcta ataaaagagc agaggtcgac aacaatcttg cattcatttc
     1201 gggcaatcta aaaacgtctt ttgcaatcaa agtgttcaaa atattctctc ctttgtcagt
     1261 taataataaa aggataccat aaaatcacca atgtgtcatg acacattggt gatttacata
     1321 cactggcatc taacaattaa aaaaagggtg agaagactgc ttttataaac tgtaaaaaag
     1381 agattgtacg aataaaaaaa atagaggcgg agaatactta tcgtatcatc cgcctttg
//
LOCUS       DAGCYL010000273         1410 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.286, whole genome shotgun sequence.
ACCESSION   DAGCYL010000273 DAGCYL010000000
VERSION     DAGCYL010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.286"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            198..1238
                     /locus_tag="MB216_002731"
     CDS             198..1238
                     /locus_tag="MB216_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBT4527504.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          572 a          190 c          221 g          427 t
ORIGIN      
        1 aattataaat aatatttatt taaagtagtt cttaatgact ttaaaggtag tacataattc
       61 attttatttc tgattttagg tagtatttaa ttaaaaaaca gttttaatgc gtttgcattt
      121 tttatttatc tttgctattt tcgaaaagag taggtaattt tacataaata aaacaacgtg
      181 aaagtagagg atctacaatg actgatttta aattttttaa ggcggataga gtttacaacg
      241 aattatttta tcaatttcca aaagtcttta ttgtttctga cgaatacaaa aaaatgaaag
      301 attcaactaa gattgcctat atgcttttaa aagcaagatt agagatcgca atcagcaaac
      361 ggcaaatcga tgaagaaggt aatgtttatt ttacttatac gacaaatgaa ctatgtagag
      421 tattaaactg ccaaaaacaa aaagcgatag caatcaaaaa agagttggaa tcctttggtt
      481 tattattaca aaagcagatg ggatttaaca aacagttagg gaaaaataat cctaatagac
      541 tatatctagc agaattaaaa gtctcagaaa atgatatcta cttactcgaa aaatttgata
      601 gagagaatag ggaaaacgtt gataaatcag agggtatgaa aatcataccc accctcgacg
      661 aaaaatcaga cgctgaatcc cttggggctc aagagggtat gaaaatcata ccgtgccaaa
      721 acgttgataa atcagagggt atgaaaatca taccagaact taataataat atattagaca
      781 ctaatagaca caatatagac actgaaaaag accgcctaca agatcaattg ttgttagaca
      841 attttgagac aattatgaca aacgacagca ttgctacgtt tgtccctgaa cgagtattaa
      901 atttgataaa aacattttct tcaagttaca gtgaagctca aaaaaccgtc cagactattc
      961 ataatgcaaa gaaaaaagct gaaatagaaa gtggtatttc gatagttttt gaagaactcg
     1021 atagttatta tgtcaatgca gaacaagaat tatacacgac actgttaaaa gcctatcaaa
     1081 aattaaaaac cgaaaaagtc gaaaatatcc agaacctgat ttttgtctat gtaaaaaatt
     1141 ggtttatcga aaaaccaata gctgctaaag tatcaagtga aaaacgtttg aattatgaaa
     1201 gctccccaag cactattacg aaagactggt tagagtgaaa ctttactgac aaagtgattg
     1261 ttgcttcatg gagtaacatt aaaattaaaa attttttatt attctaatta tattacgaat
     1321 attacaaaat aatagctatt ttaatagaat ttataagtta aaatattatg ataaaaatca
     1381 caaacgtagc cttttacgtc ataagtgtta
//
LOCUS       DAGCYL010000274         1373 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.287, whole genome shotgun sequence.
ACCESSION   DAGCYL010000274 DAGCYL010000000
VERSION     DAGCYL010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.287"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(42..113)
                     /locus_tag="MB216_002732"
     tRNA            complement(42..113)
                     /locus_tag="MB216_002732"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(79..81),aa:Gln,seq:ttg)
     gene            complement(123..195)
                     /locus_tag="MB216_002733"
     tRNA            complement(123..195)
                     /locus_tag="MB216_002733"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:His,seq:gtg)
     gene            complement(257..341)
                     /locus_tag="MB216_002734"
     tRNA            complement(257..341)
                     /locus_tag="MB216_002734"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(305..307),aa:Leu,seq:caa)
     gene            complement(362..432)
                     /locus_tag="MB216_002735"
     tRNA            complement(362..432)
                     /locus_tag="MB216_002735"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(398..400),aa:Cys,seq:gca)
     gene            complement(478..549)
                     /locus_tag="MB216_002736"
     tRNA            complement(478..549)
                     /locus_tag="MB216_002736"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Gln,seq:ttg)
     gene            complement(561..633)
                     /locus_tag="MB216_002737"
     tRNA            complement(561..633)
                     /locus_tag="MB216_002737"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(598..600),aa:His,seq:gtg)
     gene            complement(636..709)
                     /locus_tag="MB216_002738"
     tRNA            complement(636..709)
                     /locus_tag="MB216_002738"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(675..677),aa:Trp,seq:cca)
     gene            complement(716..796)
                     /locus_tag="MB216_002739"
     tRNA            complement(716..796)
                     /locus_tag="MB216_002739"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(760..762),aa:Tyr,seq:gta)
     gene            complement(798..873)
                     /locus_tag="MB216_002740"
     tRNA            complement(798..873)
                     /locus_tag="MB216_002740"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(838..840),aa:Phe,seq:gaa)
     gene            complement(909..981)
                     /locus_tag="MB216_002741"
     tRNA            complement(909..981)
                     /locus_tag="MB216_002741"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(946..948),aa:Asp,seq:gtc)
     gene            complement(991..1063)
                     /locus_tag="MB216_002742"
     tRNA            complement(991..1063)
                     /locus_tag="MB216_002742"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1028..1030),aa:Val,seq:tac)
     gene            complement(1079..1150)
                     /locus_tag="MB216_002743"
     tRNA            complement(1079..1150)
                     /locus_tag="MB216_002743"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1115..1117),aa:Glu,seq:ttc)
     gene            complement(1165..1253)
                     /locus_tag="MB216_002744"
     tRNA            complement(1165..1253)
                     /locus_tag="MB216_002744"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1217..1219),aa:Ser,seq:gga)
     gene            complement(1293..1366)
                     /locus_tag="MB216_002745"
     tRNA            complement(1293..1366)
                     /locus_tag="MB216_002745"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1330..1332),aa:Asn,seq:gtt)
BASE COUNT          393 a          364 c          310 g          306 t
ORIGIN      
        1 gtctgccaat tccgccacgc cggcaaattt aaaaataata actggggtag ctggattcga
       61 accaacgcat gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa
      121 agggcgaatg atgggaatcg aacccacgaa tgccggagcc acaatccggt gcgttaacca
      181 cttcgccaca ttcgccatca taaaagatgt tatttaattt ctcaataaaa aactaagatg
      241 aagacatctt agtcaatacc ggcggccggg gtcgaaccgg cacgccctca acgggcactg
      301 gattttgagt ccagcgcgtc tgccaattcc gccacgccgg caagttgatt tattagtact
      361 aaggcggtaa ccggatttga accggtgatg aaggttttgc agacctctgc cttaccactt
      421 ggctataccg ccattatact attatcaatc tacaaaataa agaataaatt atagaaactg
      481 gggtagctgg attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc
      541 tataccccaa gaataaaaag ggcgaatgat gggaatcgaa cccacgaatg ccggagccac
      601 aatccggtgc gttaaccact tcgccacatt cgccatggca ggggcagcag gaattgaacc
      661 cacactaacg gttttggaga ccgctgttct acctttaaac tatgccccta taaaatggag
      721 gagagtggat tcgaaccact gaaccctaag gaacggattt acagtccgtc gcgtttagcc
      781 acttcgctac tcctccatgg tggcgcggga cagaatcgaa ctgccgacac acggagcttc
      841 aatccgttgc tctaccaact gagctaccgc gccatatttt aaaattatta attgaataac
      901 aagaacgacg gttccgacgg gaatcgaacc cgcgatctcc tgcgtgacag gcaggcatgt
      961 taacccctac accacggaac cagttcatta tggaggttaa cgggatcgaa ccgctgaccc
     1021 tctgcttgta aggcagatgc tctcccagct gagctaaacc tccaacattc aattaaaatg
     1081 acccgtacgg gattcgaacc cgtgttaccg ccgtgaaagg gcggtgtctt aaccgcttga
     1141 ccaacgggcc aggtaaattt gctacggaga gtaagggatt cgaacccttg agacagtttg
     1201 caccatctac atgatttcca atcatgctcc ttcggcctct cggacaactc tccagagtga
     1261 aagaagctca atactctttc tcaaaatgag aactccggca gtaggactcg aacctacgac
     1321 atcatgatta acagtcatgc gctactacca actgagctat gccggaataa cat
//
LOCUS       DAGCYL010000275         1371 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.288, whole genome shotgun sequence.
ACCESSION   DAGCYL010000275 DAGCYL010000000
VERSION     DAGCYL010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.288"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1133)
                     /locus_tag="MB216_002746"
     CDS             complement(<1..1133)
                     /locus_tag="MB216_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBT4527505.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQY"
     gene            complement(1149..1265)
                     /locus_tag="MB216_002747"
     CDS             complement(1149..1265)
                     /locus_tag="MB216_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBT4527506.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT          400 a          284 c          209 g          478 t
ORIGIN      
        1 taatattgta gaagcgggtc actgtcggag atttctaaaa gtgcatcaag taacatttcc
       61 ctttgttgag gtttgctcgg ttcaacagtt gtttgcagca gagggagcgg attttcaatt
      121 ctctctctct gtggcaatag ctttgtatct ccaagaacac tatttagctt caaaaactca
      181 ttttgcaaaa taacaatttc cccggaataa gccttatcaa ttttacataa ttcaccattt
      241 attgaagtat acatttctgt aatttttatt ttttcctttt ccgatattct aaccgaatcc
      301 cgcaaatgca ggattccgcc ataaaggcgt acatatgcaa gacgttgtct ttcttccgaa
      361 tattcaattt tgaagacatt tccgcaaagt tcagacttct ttctgtatgt tgatgaataa
      421 aatttattcg ttatcacttc tataagctgc tcaatcccta tgttgctttt tgcgcttcca
      481 tgataaacag ggtacaagga gcaattctga aatcttatgc tttcctcttg ttcgagttcc
      541 aatgcttcta atgatttacc ggacatatat ttctctaaaa ggtcatcgtt tccctctatt
      601 accgtatccc attgttcaga ttcggtaaag ttcgtcacac acatattagg atacagttct
      661 accttctgtt tgattacaat ttcggcagaa agtttctctt taatatcctg ataaaccgtt
      721 gataaatcaa ttccattttg gtcaatctta ttgataaaaa agattgtggg aatccccatt
      781 ttcctaagtg catgaaataa tatacgagtt tgtgcttgta cgccatcttt tgcagaaatc
      841 agtagaattg ccccatctaa aactgataat gaacgatata cttctgctaa gaaatccata
      901 tgtcctggcg tgtctatgat gttcaccttc gtattttccc actgaaaaga ggttattcct
      961 gtctgaattg taattcctct ctgacgttct aaaagcgtat tatccgtcct cgttgtacct
     1021 ttgtccacgc ttcctaattc tgtaatcgct ccactgttat ataataagct ttctgttaag
     1081 gtagtttttc ccgcatcaac atgagctaaa actccaatat taataatttt catgtgattt
     1141 tcctccattc aaaagcccaa aagggcataa aaatcccagt gataaatact cttatcactg
     1201 ggatttttat gcataaccat aggcatacaa agcatacaga tattctccgg atactttaga
     1261 atcacatgat aaaggtattc ttaaactggg tgcaaaaaac taagccctcc taaaaaagga
     1321 catccaatta tttgttccca ctatcaaatt gacagtttat ttaagaatac c
//
LOCUS       DAGCYL010000276         1370 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.289, whole genome shotgun sequence.
ACCESSION   DAGCYL010000276 DAGCYL010000000
VERSION     DAGCYL010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.289"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..114)
                     /locus_tag="MB216_002748"
     CDS             complement(<1..114)
                     /locus_tag="MB216_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4527507.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTP"
     gene            184..345
                     /locus_tag="MB216_002749"
     CDS             184..345
                     /locus_tag="MB216_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBT4527508.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            364..>1370
                     /locus_tag="MB216_002750"
     CDS             364..>1370
                     /locus_tag="MB216_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4527509.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     I"
BASE COUNT          544 a          130 c          244 g          452 t
ORIGIN      
        1 aggcgtaagc tcatactctc gaataatttc tgcgcgaggc ttaccagcga gatagagatc
       61 gacgatttgt tgtttgaatt cttttgaata ggttcttcgt tgacgtcttg gcatgaaaaa
      121 tcctccagtg tgttttttat tattctacac accttatttt ttctgtctag tctagtgtag
      181 ccgattcagg ataaggataa gtgtattaat tgtggaaaat gtaaaaaagt ttgccaaatg
      241 aataaagatt cagttgagaa ttgcaatcat ctggagtgta tcagatgtgg acgatgtaaa
      301 aatgcttgtc cagtggatgc aattagttat ggagtaagaa aatagagagg agtagttagg
      361 aaagtgataa aaaatgctat agcatatatt acaagaaaga aaaatagaac tttaataatt
      421 tttatcatat taactatagt tctttcttgc ttgtattctt gtttaacaat aatgaaatca
      481 agcaatgaaa tagaaaaggc tttatatgaa agttctaatt cttcaatatc aataacaaaa
      541 aaagatggta aatattttaa tgttaatcaa tttaaagata ttgaaaaatt aaaagaaatt
      601 gaagaaaaaa taattcaata tgatggatta gcaaaattaa aagatgctaa agtagttagc
      661 ggagaacaaa gaataaatag agaagattta tctgacgaat ttaagaatgt tgtttcactc
      721 gaagctacaa ataatactaa aagaaatatt ttatttagta gtggagtatt tacaattaaa
      781 gaaggtaaaa atatagaaga aaatgataag aattcaatta ttgttcatga agaatttgct
      841 aaacaaaaca atctaaaatt aggtgatgaa gttgatcttg aattactaga tattgaaaaa
      901 agtggaaaaa taaaaagtca taaatttaaa attataggga tcttttctgg taaaaaacag
      961 gaaacatata caggattatc ttctgatttt agtgaaaata tggtgtttgt agattattca
     1021 acaagccaag agatattaaa taattcagaa aataatgaaa ttgcaaataa aattttaatg
     1081 tattctggta gtgcagaatc tacagactta gccttaaaca aattgaaaga gcttaaaatt
     1141 gatgagtcaa agtattttgt tgaaaaagat tctaatgcgt ttgaagagtc tttagagtca
     1201 gtgagtggaa taaaatatat aataaaagta atgacttatt ctattatgtt aggtgggatg
     1261 gttgttcttt cattaatctt gattctatgg ttaagggaaa gaatttatga aataggtata
     1321 ttcttatcta ttggaacatc taagatacaa attataatgc aatttatatt
//
LOCUS       DAGCYL010000277         1366 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.290, whole genome shotgun sequence.
ACCESSION   DAGCYL010000277 DAGCYL010000000
VERSION     DAGCYL010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.290"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(93..392)
                     /locus_tag="MB216_002751"
     CDS             complement(93..392)
                     /locus_tag="MB216_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBT4527510.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            743..>1366
                     /locus_tag="MB216_002752"
     CDS             743..>1366
                     /locus_tag="MB216_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBT4527511.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIK"
BASE COUNT          459 a          222 c          194 g          491 t
ORIGIN      
        1 aaaaaaatac agactctgtt caaataaatg aacctagcct gcattatgag attgactata
       61 ctttttaatt cattgagatg aataactcgt ttttataaat gcccaaaagt aatcggtgcc
      121 gaagccattc ctcgacctaa ttctttttca aattttccta ttgttgatat ctccatataa
      181 aacggtgata ctggtttaga caattctccc ctgattgcta acggacgtaa actacgttcc
      241 aaattattta tgactttagg ataatactga cctttttcta agcctgtctt tgcgtctatc
      301 aaaagtatac gttccttttc tttgatctct ggatttaaaa cgagatcata taattttagt
      361 accatgtttt ctatttcttc acttttagac atcacgattt cctcccaaaa atatatttct
      421 tcccactaca ttctataaac aaagtatacg ctacatctag atttttttaa atcattactg
      481 gattgttaaa acctttcaat acactgttat ggcaacgatt tttattttaa aaacattcta
      541 aattaaatca gtaaaaattt ttattctaag atttttatta gaattacgcc taatttgtgt
      601 ctctcagggc aattctaaga acgtttcaag agatagcgac actatcgtta tcgaaaaagg
      661 cgaaaaccta tctcattttc tgctaatgta agttggttta gcttttgtta atatgacttt
      721 atgaaaatgg aggtaattaa atatgatttt tcctaaagtt aatgaagcaa gcttgaataa
      781 aggatccttt tttcatatct atgaaaacga agattttgaa ctgtataata tcctttcaca
      841 aaaagaggtc atcgaaaacc aaataatctg ccatccaatt atttacaaat ctattttcaa
      901 gtatgcattt tttgcttcca atgattttca aagaatggag attaccgact gtatattcga
      961 aaactgtgat ttttcaggct gtatattgac aaaaagcatg ttgcatcgcg tgaaatttgt
     1021 caattgtaaa ttaacaggta ctaaattgat ggaaagttat attgggaaca cgttattcga
     1081 gaattgtaaa atggactatg taaatttatc tggttcaaac ataaaagaaa gtacctttga
     1141 gcactctgtc ttatcaagcg cagattttgt agactgttcc ctcactaaaa taatgttttc
     1201 tactaatgat ttagaaaatg ctagcttttt tgatactgat ttaaaaaata tcgatttaag
     1261 tagcaactcc tttgaaatga ttgaagtacg ctcggaacat ctaaaaaatt gtacgcttaa
     1321 tcagttgcaa gcacttggtt ttgctagaaa atttttacaa ataaaa
//
LOCUS       DAGCYL010000278         1364 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.291, whole genome shotgun sequence.
ACCESSION   DAGCYL010000278 DAGCYL010000000
VERSION     DAGCYL010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.291"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(140..1288)
                     /locus_tag="MB216_002753"
     CDS             complement(140..1288)
                     /locus_tag="MB216_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4527512.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAGCYL010000279         1364 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.292, whole genome shotgun sequence.
ACCESSION   DAGCYL010000279 DAGCYL010000000
VERSION     DAGCYL010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.292"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            80..>1364
                     /locus_tag="MB216_002754"
     CDS             80..>1364
                     /locus_tag="MB216_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBT4527513.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          465 a          263 c          257 g          379 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcaccacaa aataaacaac
       61 tgagaggacg atacgctata tgaatgattc tatcaaaaaa atgctgagaa taacagagaa
      121 agatttgatg attacagagg tctcttacga gacccttcag aagaaaaaga cgttggtcgt
      181 cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt tgtggttcta ctgtggtaga
      241 tggaaacggg aaagcaatta tagtgaaaaa tggaaaaaag gaaacgattg tccgttttga
      301 acaatacaat catatgcctt tggttatgcg cctaaaaaag caacgctata cctgtaaaaa
      361 ctgtcgcacc cattggacgg ctcaaagtta ttttgtccaa cccagacatt caatcgcaaa
      421 tcatgttaga tataaaattg cttctttact gactgaaaaa gtatctttat cttttattgc
      481 gaaaagctgt caggtatctt tgaccaccgt tattcgtaca ttgaaagagt ttaaaagcta
      541 tttaccaaag caatctaaga agatcctccc cagagtattg atggttgacg aatttcgttc
      601 gcatgcttcc atagaagata agatgagctt tatttgcgcg gatggcgaaa caggaaaatt
      661 aatagatgtt ttgcctacgc gtaaattacc tcgattaaca agctatttct taggttgtac
      721 caatccagaa gaagtagaat tcttggtgac agacatgaac gccgcctact tccagctcac
      781 caaacgtgtt ctgccaaatg cgaaagtcgt gattgatcgg tttcatattg tcaaacacat
      841 gaatcaagcg ttcaatgagt tgcgtatccg tgaaatgaat gaacttcgta aagccggaca
      901 gaaaagccag gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa
      961 catcaaccat tatgaataca aaacatggaa aagtttccga gcaccaaaat acccatttct
     1021 tactgaagca atgatgattg atcgattgct tgagttttct ccgcccctaa aggaggcgta
     1081 tccctttttt catgaattag ttgaagcctt tcgagacaaa gaccctgact tatttttctc
     1141 cttattggca gaacttcccg aaacgttaga tgacagcttt cgggaaaagc ttcaaaacct
     1201 tctgacctat gaagaaggca tcaccaacgc aatgatctat ccttattcca atggaaaaat
     1261 agaagcgaag aatacccaca taaagacaat gaaacgagta tcctacggat ttaaatcatt
     1321 tgagaacatg agaattagaa tctttttgat caatcaatta atca
//
LOCUS       DAGCYL010000280         1355 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.293, whole genome shotgun sequence.
ACCESSION   DAGCYL010000280 DAGCYL010000000
VERSION     DAGCYL010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.293"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..909
                     /locus_tag="MB216_002755"
     CDS             <1..909
                     /locus_tag="MB216_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBT4527514.1"
                     /translation="SDSFLAAFYLYTAKYWIDDSRKTIKYAKRKKQFYFQTWHGTPLK
                     KIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSEKYVSAFNIVPSKILEVGTPRN
                     DELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQLEWLGVDNLREFFKKQQQELN
                     LMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLEMLFEEIDVLITDYSSIFFDF
                     ALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDSYGLVDVFKNQQEKLLKSSQE
                     LLDYIGNYETGASTEKVLELLERDIK"
     gene            906..>1355
                     /locus_tag="MB216_002756"
     CDS             906..>1355
                     /locus_tag="MB216_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBT4527515.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIAGLTLDISWFFQGIEEFSKVVIRNTVIKL"
BASE COUNT          488 a          168 c          221 g          478 t
ORIGIN      
        1 tccgattctt ttttagctgc cttttatttg tatacagcta agtattggat agacgatagt
       61 cggaaaacga ttaagtatgc aaaaaggaaa aaacaatttt attttcaaac atggcacggt
      121 acgcctttaa aaaaaattga atttgatgca aaagataagt tacctaaacg ttacctaagc
      181 tatgcgaaaa aagatagtga gtcaattaca tatttattat ctggtaatcg ctatagttct
      241 gagaagtatg tttcagcatt caatatcgtt ccttctaaaa tattagaagt aggaactcct
      301 agaaacgatg aattagcctc tagtaaagag caagtgtttg acttgaaaaa gaaacaaatc
      361 aatgtcttat ttgctccaac ttttcgaaat aatattgagg acaacggaat tacgcaacta
      421 gaatggttag gtgtggataa cttacgtgaa ttttttaaaa agcaacaaca agaattaaat
      481 ttaatgacta aatttcatcc taatgttcat agtaaattag caactgattc taaaagtata
      541 gaatatatga aaaaacatca aattacttta attaatgatg ggattcctct tgaaatgctt
      601 tttgaagaga tagatgtttt aatcacagat tattcatcta tttttttcga ttttgcgcta
      661 acaaaaaaac caattattct ttttaactat gatgaaaaag aatatagctt agaaagaggt
      721 ttttatatac agttaagtga gttaccaatt ataagcgtac aagatagtta tggccttgtt
      781 gatgttttta aaaaccaaca agaaaagctt ttgaaaagtt ctcaagagtt acttgattat
      841 attggaaatt atgaaacagg ggcaagtaca gaaaaggttc ttgagttgtt agagagggat
      901 ataaagtgaa aaaaacagta aaagatatat tttataatgc gatatatcaa gtctttttga
      961 ttgttttgcc cttacttact atacctatac ttagtcgtag aataggttca actggtttag
     1021 ggatttatgg gtatgttttc tcaatatcac aatttttaat gactgtcatt gctgtgggaa
     1081 tgaatccttt tcgtattcga aatatcgcta aatcaaggaa agataaaaaa gcactatctt
     1141 tacaattctg gaatatttac tttcttcaat ttttaatagg cttgagtgtt tcctttctct
     1201 atatagccta tatttatttt tttgatgtaa agtataaaaa tttatactta attcaattga
     1261 tatttattgc agggctaaca ttagatattt cttggttttt tcaaggaatt gaagaatttt
     1321 cgaaagtggt tattagaaat acagttatca aatta
//
LOCUS       DAGCYL010000281         1342 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.294, whole genome shotgun sequence.
ACCESSION   DAGCYL010000281 DAGCYL010000000
VERSION     DAGCYL010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.294"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(740..1081)
                     /locus_tag="MB216_002757"
     CDS             complement(740..1081)
                     /locus_tag="MB216_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBT4527516.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1081..1311)
                     /locus_tag="MB216_002758"
     CDS             complement(1081..1311)
                     /locus_tag="MB216_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBT4527517.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT          412 a          224 c          196 g          510 t
ORIGIN      
        1 tattgatagt gtacctaaaa gctctactga cataagatcg tgtaatcggt aaagtaattt
       61 tattaattat acgggatgta atcaattaga gaataaacta atcataaaag gtacttttac
      121 attcataatt gtcgctttga gattctactt tggttcacta tctgacccaa ttgcacattt
      181 tcgatcatta caagtattgg tgtcgttaga acaactaaat aaaaactagc ggtagatttt
      241 tcagaatcta tgctaatttc tgtatggatg ttccagtgat ggcaaatttc tcttttcatt
      301 gtttttcttg tcgcatgata tgcctttttg tgttattcag ttaaactata gaaattcttt
      361 cctagacaga tcttgatccc aaattttacg tttttgagca tcgatatagt ttaaaatgac
      421 attactcata tctaaagagt ttagattttt taggactaaa actgtcagtt gttctttttc
      481 ttctttctgt taaagtattg tcgcgacttt tcgtatacgt taagatatgt cgtgaaaaaa
      541 caagttttgt gacacaaaaa aacgaatgac ttgaggaaat cacctcaatc attcgtttaa
      601 aatgctatat attctttagt ttaagatacg gaaattaatg ccatcgacca cactagtaga
      661 atgaacgaca tttacttggc ctttttttaa tttcgttatg tttagcatat aaaaatatta
      721 tagagtttac ttacaatgct taaaacatat actgtataat ctgatcaatt ttagccatgt
      781 cttgcaatgg taagttttct atttttttga gttttcgttc cttaaaatca agagatttta
      841 attgtgtaat aattacttgt ccttttatat ctagctcttc aggtaatgag taacgtgtcg
      901 gtaaattctt tatagtggaa gtaattggac atatgaccga aaacattgtt ttacggttaa
      961 attcataacg agagacgact agtcctggtc gtcttttttg aatttctttt cctacggagg
     1021 gatcaaaatc aatccataca atatcacctt ttttaggaat ataaattctt tcttctgaca
     1081 ctataaaatt tctctccctt caggagttaa atcttcccat tcgtctttct cataatactc
     1141 ggcctcttcc ccaccactaa atggatcttc aatttttgga actaacgtaa ttgttccatc
     1201 atcagaatac acaacgatat attcttggtt ttctgaaggt ttctttccgt tatcagaagg
     1261 taaagttacc actacagaac ttccttgtaa tctagatttt gtaacaagca ttatcatcac
     1321 tcccttaata aaagtatact at
//
LOCUS       DAGCYL010000282         1330 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.295, whole genome shotgun sequence.
ACCESSION   DAGCYL010000282 DAGCYL010000000
VERSION     DAGCYL010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.295"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(24..431)
                     /locus_tag="MB216_002759"
     CDS             complement(24..431)
                     /locus_tag="MB216_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527518.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(450..1268)
                     /locus_tag="MB216_002760"
     CDS             complement(450..1268)
                     /locus_tag="MB216_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBT4527519.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          383 a          226 c          190 g          531 t
ORIGIN      
        1 aattgaatac cgcatgaaaa aaattatttt ggtaattttt caaactcttc ttgtatttct
       61 tctcttatgt gttcttcttt tgctactcct agtctatgct ggatatatga gtctgctaat
      121 gtattaacaa ttttttcgaa agaaattctc cctttttcac tttgtaactc cgtcatatag
      181 tcttgtaaaa ttgagagttt gctatttaat gccggagaga tctttttagg aactgatacc
      241 ttgtttcttt tatcaggaat tgaaactggt cggcctcttt ttttcaatgt aatatttctt
      301 gtgttactca atgcactatt gctaaagtta tattcaccgc ttattttcac atcttggcgt
      361 ttgttattgg gtaataatgg ctcaaattca ccattctcca tcattttagg aatatcaaaa
      421 tctttaggca ttacttatgc aactccttat tatttattta aaaactcttg tctagcatct
      481 agttctgcca aaatatttat aaatacttgg tgtgctttat aatcccattg atcaaaattt
      541 ccatttttgt attttcttaa atgtatacca attttagaat accttctcaa tcttttttga
      601 taatgaatga gatttttgga taccacggtt ccgtattttt caattgcttc ttctaggatt
      661 tcttcatcaa aatcatcatt tggttctacc atacaaggta caatatctat aatttgcaaa
      721 tcaagatcgt atctatcgac catgaatttt tgataattta catatttgtt gactccctct
      781 aaggattctt cagctgtttg aaatgaaatt atcgaatagt ctgaagctgc catagcatta
      841 tcggaaaatt cgctaatggt tggtggtaca tcaataaata tttcttgata attgttcttt
      901 attggaagaa gtaatttttt caagacagta acttggtcta attcattttc atgtttatta
      961 gcaaaagcgg agaatgatcg aaaactagtg tcacatgcca gaaggtcaag attttcattt
     1021 accttaataa tactacttgc taattctcca tttgcgacac catttgttat agtggttttg
     1081 atatcggtta taccgaaagt tcttgctatg acttgtgtta agttcgcttg agggtctaaa
     1141 tcaataataa gcgtttttct atttcttatc attgcattgt cataagcaag catgctgact
     1201 atcttagttt tacctactcc tcctttaaaa ttgccaactg taaatgtagt cccttgaaaa
     1261 gtatccatgt aaatcctccc aatctttaat tgataattaa agtataactt atatatcgga
     1321 aatggtcaac
//
LOCUS       DAGCYL010000283         1329 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.296, whole genome shotgun sequence.
ACCESSION   DAGCYL010000283 DAGCYL010000000
VERSION     DAGCYL010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.296"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1192
                     /locus_tag="MB216_002761"
     CDS             <1..1192
                     /locus_tag="MB216_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBT4527520.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDN
                     RSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIV
                     VVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQ
                     YHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDN
                     KTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRK
                     KENE"
BASE COUNT          531 a          191 c          287 g          320 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tctgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gtagaagttg gaggcacagt
      301 agatctaaca gataatgtta ctaacttgcc aacattacca gaaggaacaa cagtaacaga
      361 tgtcactcct gatggtacga ttgataccaa cacaccaggt aattacgaag gggttatcga
      421 agtaacgtat ccagatggta cgaaagatac agtgaaagtt ccagtagaag tgacagacaa
      481 tcgttccgat gccgataaat acaatcctga aggacaaaaa gtgactacag acttaaataa
      541 agagccagac gcatctgagg gaattaagaa caaagaagat ctaccaaaag gaactactta
      601 tacttggaaa gagaaagtag atgttagtac agctggaaac aaaaaaggga ttgtagtagt
      661 aacatatcca gatggatcta aagaagaagt agaggttact atttctgtag aagataaaaa
      721 agcaccgaat aaacctcaag ttgatcctat tacagagggt gaccaaattg ttactggtaa
      781 aactgaacca aatgcagagg tgacagttac attaccaaac gggagtcaat accatgggac
      841 agctgataaa aatggtaatt ttacagttaa agttcctaaa ttagaggcag gaacaaaagt
      901 gatagtaact gcaactgatg aatctggtaa tactagtgaa cctactaatg tagttgtttc
      961 ttcaaacgaa aaagacagtg aaaaagccgt tagtaaagat aataagactg acaatcaagg
     1021 tagcaagcaa aacaaaaata gaggcaaaag cagtcctcaa aagcagtcct caaaagctta
     1081 tcctaagacg ggagagattg atagtaatat ctttactatt agtggtggtt taatcctgtt
     1141 aggaacttta gggttattag ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa
     1201 tttcttaaat cttctctgaa aagaggaaga atctttttct tcctcttttt gttttttatt
     1261 ttgggagata ggttgttagt taaaaatgaa gatacttttc atagaaggga aaagaatgtg
     1321 attaagtga
//
LOCUS       DAGCYL010000284         1311 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.297, whole genome shotgun sequence.
ACCESSION   DAGCYL010000284 DAGCYL010000000
VERSION     DAGCYL010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.297"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(190..801)
                     /locus_tag="MB216_002762"
     CDS             complement(190..801)
                     /locus_tag="MB216_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4527521.1"
                     /translation="MGIQIISLSDLLEVAPDLESVNSILKTFKSIPHPITGQVNDVEY
                     FLHQKAIEFEKAALATTHLLFSSYKDKSILVGYFSLANKSLIMSKKNYNNLSKPQQRR
                     LCQNGSKTETGGYIVNSYLIGQVGKNYSDEAQKIDAINGTQILTIAYDTVLQAKKIIN
                     ARYVWIECENIPRLIDFYRDFGFEVIENYETNSGLVVMVMKLK"
     gene            complement(802..972)
                     /locus_tag="MB216_002763"
     CDS             complement(802..972)
                     /locus_tag="MB216_002763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527522.1"
                     /translation="MATKSFTEEYRFTKKNANSLINALSNDKSPRKQLVTNLKVIDDK
                     EKIKNIFFGSNS"
BASE COUNT          449 a          226 c          154 g          482 t
ORIGIN      
        1 taaatacttc atatcaatca tttattcaga aacaaactat cttgcaggat ctatacgagt
       61 taattacaga gcaaaaaagc ctcaaccaag gatgactggt tgaggttttt gttttacaaa
      121 tttaaatctt tttaagttca ctatacgctt gtttaacctt gtaatagata catgcaataa
      181 ttagttttac tattttaact tcataaccat tacaactaat ccactgtttg tttcataatt
      241 ttcaataacc tcaaaaccaa agtctctata gaaatcaatt aacctcggaa tattttcaca
      301 ttcgatccat acatatctag cattaataat tttttttgcc tgcaaaaccg tatcataagc
      361 tatcgtcaat atctgggttc catttatagc atctatcttt tgagcttcat cagaatagtt
      421 tttgccaact tgtcctatca aataactatt aactatgtaa ccaccagttt cagtttttga
      481 accattctga cacaatcttc tttgttgggg cttactgaga ttgttataat tttttttgct
      541 cataattaag gatttgttag ctaaagaaaa ataaccaact aatatagatt tatctttata
      601 tgagctaaat aaaagatgcg tagttgctaa agcggctttc tcaaattcta ttgctttttg
      661 atgcaaaaaa tattccacat cattaacttg accagttata ggatgtggaa tagatttaaa
      721 tgtctttagt atgctattta cactttctaa atccggagca acctctaata aatcacttaa
      781 actgataatc tgtattccca tctaactatt acttccaaaa aaaatatttt ttattttttc
      841 tttgtcatcg attactttta aattagttac caactgtttt ctaggtgatt tatcattaga
      901 taatgcatta attaaagagt tagcattctt ttttgtaaac ctatactcct cagtaaaact
      961 tttagtagcc ataacaatcg cctccgttta tgtaccaaca caaaaatata taggaaaaaa
     1021 cacgtcaaaa taacgtactt gtacaaaaca atcatacatc attttacgct ttaactttcc
     1081 tttgttgtaa gttcaatata ccatcattgg tttaattgta caagagaaac ggctaaaaaa
     1141 tcagctaatt ttgttagcaa ctttgttagc atatctgaca gtagatcaat agtaaaagtg
     1201 cctgtatata aggaaaaact gtatttttac tatctaaatt cgtaaacatt tttgcttaca
     1261 aattagctaa taatattagc aactatattg aaaactatat gtactgcccc t
//
LOCUS       DAGCYL010000285         1291 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.298, whole genome shotgun sequence.
ACCESSION   DAGCYL010000285 DAGCYL010000000
VERSION     DAGCYL010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.298"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..265
                     /locus_tag="MB216_002764"
     CDS             <1..265
                     /locus_tag="MB216_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBT4527523.1"
                     /translation="NYWLSVITIKGINTNHLIDELDKNNIEARPVWKPMHLQPVFKNY
                     DYIGDDVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            255..875
                     /locus_tag="MB216_002765"
     CDS             255..875
                     /locus_tag="MB216_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBT4527524.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNTPEKFKGS"
     gene            1002..>1291
                     /locus_tag="MB216_002766"
     CDS             1002..>1291
                     /locus_tag="MB216_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBT4527525.1"
                     /translation="MCRVLNILFQYENIIKKYKEKRVMAKYYNKKILIMANNDDGLYR
                     FRGMLIKNLVDQGNEVIVSLPKGKNISKLIELGCQIIETPIDRRGINPKTDF"
BASE COUNT          507 a          158 c          227 g          399 t
ORIGIN      
        1 aaattattgg ttaagtgtca taacaataaa gggaataaat acgaatcatc ttattgatga
       61 gttagataaa aataatatag aagcccgccc agtatggaaa ccaatgcatt tacaacctgt
      121 tttcaaaaat tatgattata ttggcgatga tgtagcagaa actttatttg acttaggagt
      181 atgtttacca tcagatacaa agatgacaga tgaagattta aaatatgtag tatctacgat
      241 taaaagagtg atcaatgatg cgtaaaaaaa ctttctatca gctttttggg aaaagaattt
      301 tagatatcct attgtcagga attgctctca tcgttttatc gccgataatc ttaattgtag
      361 gttttttagt aagaattaaa cttggttctc caattatctt caaacaagaa cgaccaggaa
      421 aaagtgaaaa aattttttcc atgtataaat ttcgtacaat gactgacgaa agagaccaca
      481 atggagaata tttacctgat gaaattcgac taactaaatt tgggaaaatg cttagagcaa
      541 caagtctgga tgagctaccc gaactttgga atattttgaa aggtgatatg tctatcgtag
      601 gacctcgtcc attattagtg gagtatttgc cactatatag tgagaagcaa cggaaaagac
      661 acaacgtaag accaggatta actggactgg ctcaggtcaa tggaagaaat gctattagtt
      721 gggaagaaaa gtttgattta gatgtttatt atgttgataa aattagtttc tttaatgatc
      781 ttattattat aattcagaca tgtaaaaaag taatcaagaa agagaatatt aatacaatca
      841 ataacaatac cccagaaaaa tttaaaggga gttaaataca gcagcttaat gcaagttttg
      901 tgacttgcat tatatttagg tataaatcaa agcgagtata ttagaattat ataacatatg
      961 ttaaaaaaac gcatagctaa taaaagtatg attttaaaaa tttgtgtaga gttttaaata
     1021 tactgtttca atacgagaac attataaaaa agtataagga aaagagagtt atggcaaagt
     1081 attataataa aaaaattctt ataatggcaa acaacgatga tggtctttac cgttttcgag
     1141 gaatgctcat aaaaaactta gtagatcaag gtaatgaagt tattgtatct cttccaaagg
     1201 gcaagaatat ttctaaatta attgaacttg gatgtcaaat tattgaaact ccaatagata
     1261 gaagaggaat taatccaaaa actgacttta a
//
LOCUS       DAGCYL010000286         1271 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.299, whole genome shotgun sequence.
ACCESSION   DAGCYL010000286 DAGCYL010000000
VERSION     DAGCYL010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.299"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            46..105
                     /gene="ermCL"
                     /locus_tag="MB216_002767"
     CDS             46..105
                     /gene="ermCL"
                     /locus_tag="MB216_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HBT4527526.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            153..887
                     /locus_tag="MB216_002768"
     CDS             153..887
                     /locus_tag="MB216_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HBT4527527.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          472 a          198 c          191 g          410 t
ORIGIN      
        1 cttgtgctat aattattcta atttcataaa aaggagaatt aatatatggg catttttagt
       61 atttttgtaa tcaacacagt tcattatcaa ccaaacaaaa aataagtggt tataatggat
      121 tgttaatata aacctcatag gagtgggtta taatgaacaa aaaaaatata aaagatagtc
      181 aaaactttat tacttcaaag catcatataa atgaaatttt gagaaatgta catttaaata
      241 caaatgataa tattattgag attggttcag ggaaaggtca tttcacgttt gaattagcta
      301 aaaggtgtaa ttatgtaacc gccattgaaa tagatcctaa attatgtagg ataactaaaa
      361 acaaacttat tgaatatgag aacttccagg ttatcaataa agatatttta caatttaagt
      421 ttcctaaaaa taagtcatat aagatatatg gaaatatacc ctacaatata agtacagata
      481 taattcgaaa aattgttttt gaaagcacag ctacagaaag ttatttaata gtggaatatg
      541 gatttgctaa aaggttgcta aatacaaatc gttcactagc actattttta atgacagaag
      601 ttgatatatc catattaagt aaaatcccta gagaatactt tcatccaaaa cctagagtta
      661 atagctcgtt aattgtatta aaaagacacc cttcaaaaat atcactcaaa gatagaaaac
      721 aatatgaaaa ttttgttatg aaatgggtta acaaagaata cataaaacta ttttccaaaa
      781 accaatttta tcaagcctta aaatatgcaa gaattgacga tttaaacaat attagctttg
      841 aacaattctt gtctcttttc aatagctata aattatttaa tagataagtt aaggggtgca
      901 taaactgcat cccttaactt atcttatttt gcttaaacaa tcttggcccg taaactgcat
      961 aactcatatg taaatcgctc ctttttaggt ggcacaaatg tgaggcattt tcactatttc
     1021 cggcaattac ttccatgtaa agtataaccc actatacttt gcaagcaaag tgtgggctcg
     1081 ccgaggggtc ttgcagacgg ctctttcggc tcttttgagc cgagtatcag cgcaccctta
     1141 tggtgcaatt agctgatacc tgtttgacga tttgctgccc taccttttcg gtaggtatgc
     1201 aaacctcaaa ccaaaataaa aacccccttc gacttcgtca gggggttttt atttatataa
     1261 atctccgtaa a
//
LOCUS       DAGCYL010000287         1268 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.300, whole genome shotgun sequence.
ACCESSION   DAGCYL010000287 DAGCYL010000000
VERSION     DAGCYL010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.300"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            20..1186
                     /locus_tag="MB216_002769"
     CDS             20..1186
                     /locus_tag="MB216_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4527528.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT          457 a          216 c          241 g          354 t
ORIGIN      
        1 ttttttaagg aggaaaatca tgttagagaa aactttatac aatcaattat tttcacgttc
       61 tttctcgctc cctgtcgaag taaattattg ggacggtaca acgaaacaat acggagatac
      121 ggacactcct ccccaaatca aaatcaatat ccatgaagaa atcccaatga aagagattac
      181 aagtaacgct tctttggcac taggagaagc ctatatggat catcgaatcg agatcgaggg
      241 aagtatccaa gcattgatca acgatgctta tcagcaagca aacagctttt tacgaggaaa
      301 agattatcta aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa acaaagaaga
      361 tatccatgcg cattatgact taggtaacga cttttataaa ctttggctag atccaacttt
      421 gacttattct tgtgcttatt tcaatacacc tgaagatacg ttggaacaag cacaaatcaa
      481 taaagtccat catatcttag acaaactctt tatcaaagaa ggcgaaacgt tgcttgatat
      541 cggttgtggc tggggaacat tgatgttcac tgctaccaaa gagtataatg tcaaggcaac
      601 tgggatcaca ctaagtgaag aacaatacga tttgattacc aaaagaataa aagaagaaca
      661 tttagaagat aaatgccgtg tattattaat ggattaccgc gaactaaaag gcgaaacgtt
      721 tgatcatatc acaagtgtag gtatgttcga acatgtaggt tctgaaaatc ttgaaggtta
      781 tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc atggaatcag
      841 ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa tatattttcc ctggcggtta
      901 tgtccctggt gttactgaat tagttgataa tattacaaaa aacgatttgc aactgattga
      961 tttggaaagc ttacgtcgtg actaccaatt gacattagaa cattggacaa agaattttca
     1021 tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa tgtgggatct
     1081 atatttacaa gcttgtgcag cttcatttga atcaagtaat atcgatgtga ttcaatattt
     1141 gcttgttcaa cctggaaaca atgacatccc aatgcatcgt gcataattga ggcaaaagta
     1201 ctgtttaaag actatttaaa aagaagctaa gttttatgat gctccagaag tgaacttttt
     1261 tgtctaac
//
LOCUS       DAGCYL010000288         1264 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.301, whole genome shotgun sequence.
ACCESSION   DAGCYL010000288 DAGCYL010000000
VERSION     DAGCYL010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.301"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..946
                     /locus_tag="MB216_002770"
     CDS             <1..946
                     /locus_tag="MB216_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBT4527529.1"
                     /translation="TKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQL
                     WKPAFIILTPQLFMQVYTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVM
                     LPKISKAQAEGKSENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMT
                     INMLISCLIIVVSPIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAV
                     AATIVLVVVELLVFFFRVFFVRKEIKISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFS
                     SSFLDMAIKSIMIFIIYLPFVYFLFPDIRNEVRKIIRK"
     gene            complement(1013..1177)
                     /locus_tag="MB216_002771"
                     /pseudo
     CDS             complement(1013..1177)
                     /locus_tag="MB216_002771"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          397 a          173 c          210 g          484 t
ORIGIN      
        1 aactaaagat gatttgtgga tttatgtgtt cattacttca attactaact ttttaggaag
       61 tttagttttc tggttgagta tccacggaaa agtaggtaaa ccactcttta actctaatgt
      121 ttttaaacag ttgtggaaac cagcttttat tattttgact cctcagttgt ttatgcaagt
      181 gtacacaact ttagacaaaa cgatagttgg gtattttgtg gatccaacag cattatctta
      241 ttatgatcag tctcaaaaaa ttgctcgtat tatattggca gtcttaacat caattactat
      301 tgttatgttg cctaaaatat ctaaagcaca ggcagaggga aaatcagaaa atattttagt
      361 ttacacaaaa aaatcttttg attacacgat tattattgca ttaattcttt ttagtgtggt
      421 gtttatcaat acaaaagaat ttgttccatg gttttttggg gagaatttca aaccgatgac
      481 aataaacatg cttatttctt gtctaattat agttgttagt ccaattggtg gagtttttgc
      541 taaccaattt gcaattgcta tagaaaaaga taaggagtat gcttatccaa tgatcattgg
      601 tgcgattgta agtattattg gaaatgtagc ttttgtgcct acttatggcg ctgtagcggc
      661 tacaatcgta ctcgtggtag tagaattatt agtctttttc tttagagtat tttttgtacg
      721 aaaagaaata aaaatatcct atttctttgg gaagaacgtt gtcttatttt ttatagtcaa
      781 tcttactttg actacttcgg cctattactt tcttccacaa ttattctctt cttcattttt
      841 agatatggca attaaatcaa taatgatttt cattatttat cttccgtttg tttattttct
      901 ttttccagat atacgaaatg aagtaagaaa gattattcga aaataaaata atgttgtaaa
      961 aaagtagttc tttgtcaaat cgggctgata aggtcattta tgatcaacaa agttagtaag
     1021 atttactagc ttttaatgca tagcttatta acccgttgat tagaaaaatc cacaagcgca
     1081 tattggtaaa actgcaaaat ccataagcaa atcgtttcaa tgttttgata tgtgtgcgaa
     1141 gattctctaa ttttgtatta aaatactttt tcgaaaatgc tagttaaaag cctttttcag
     1201 atatgaaaaa ggctttttaa gatagtgaag tttcgaggga acttctttgg tcatttctcg
     1261 aagt
//
LOCUS       DAGCYL010000289         1253 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.302, whole genome shotgun sequence.
ACCESSION   DAGCYL010000289 DAGCYL010000000
VERSION     DAGCYL010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.302"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            33..1019
                     /locus_tag="MB216_002772"
     CDS             33..1019
                     /locus_tag="MB216_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBT4527530.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDTLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDENLEDDTITIPIKEEQ
                     VDVSKHTVVREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHMEDRNKKS"
BASE COUNT          463 a          204 c          286 g          300 t
ORIGIN      
        1 aatcataaat ctatttgcta ggaggaattt acatggaaga gaaaaattta acggtcatcg
       61 gaagctatag tacgagggaa gaagcattat cagtaattga acggttgcgt aacgaagggt
      121 atgaacgaga tgatatcgtt atttatacta ctgatgaagc agcatctaga ctaggatttg
      181 acggattgtc tggtatagat gttgaaacag atgaaaatcg gatggatggt gaagaagacc
      241 gttctttatg ggaaaaaata aaagatactt tttcttttga tacctatgat tctgagacgg
      301 ctacaccaga aaatgatact ttatatcaat atagaggaga tatttctgat ggaaaatttg
      361 tcatcacagt aaaaggctat cgtcagccag aaacaacaga agatacaatg gatacacaag
      421 atactgttag cccagcaagc acaatgggga ctggattaga aactggcact ggaacagatc
      481 caatggacgt aggtggcacg acaggctttc aaaatacaac ggatccattg aatatgacac
      541 caggaactga tccgatggac gtgcctggcg agactgatac aatgggtaca agcaagactg
      601 aagacatcga tcgcaaacca aatctagatg atgatacaat tcaactaaaa gaagaaaaat
      661 tagatgtaaa tacacatgat gtgacaactg gagaagtcga catccacaaa catgtggtca
      721 acgatactga aacggtagaa gtaccggtca aacgtgaaga aatcgtcatc gaacgcaaac
      781 cagtaacaga tcaatcttca cagggaacag acgaaaactt ggaagatgac acgattacta
      841 tcccaatcaa agaagagcaa gtagatgtaa gcaaacatac agttgttcgt gaagaagttg
      901 ggattcataa agaagaacat gaagatgttg aaaaagtgac agaagatgtt agccgagaag
      961 aattggatat tgacacaagc ggtgatgtac acatggaaga ccgcaataaa aaatcgtaag
     1021 cgtaagctaa agaggttgtg acagaagtgg tcagcttaaa gaaatttcgg cttatttccg
     1081 aagaagctac ttctggaaca ccgtttattc gcaacaaaaa agggctgtga caagattttt
     1141 tgtagtgacc cccaaaagtt agactttttt ggagtggaga aatccactcc ttttttcatg
     1201 ctctaaaatg atatttttac acaaccgttg cggttgtttt acgaaattgg acg
//
LOCUS       DAGCYL010000290         1242 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.303, whole genome shotgun sequence.
ACCESSION   DAGCYL010000290 DAGCYL010000000
VERSION     DAGCYL010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.303"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            69..1154
                     /gene="xerS"
                     /locus_tag="MB216_002773"
     CDS             69..1154
                     /gene="xerS"
                     /locus_tag="MB216_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBT4527531.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          495 a          209 c          190 g          348 t
ORIGIN      
        1 actctataat tggtatagtc caattataga gatactcctc tcccctactt atcgaaagga
       61 tgaataacat gaaaaataca gaaatatatc aaaaaataga tacaatcata cttcatatgc
      121 ccgattatat aaaagatttt gttcaagatc gtgaggataa agatcaatca cctcgaacta
      181 ctttagaata tctaaaaaat tataagctct tttatgaatg gcttctgagt gagtctatcg
      241 ttcccgatac ttcaatcacc tcgataagac agttaactgc ctatgatcta aacttgtaca
      301 aatctcattt gaaacgtcgc acaaaggaaa atgtaaaaaa agagacaaca aaaactaagc
      361 tcaaagataa taataactta ggtctatcta cttctactat caatagaaat atcacagcat
      421 taaaggttct atttaaatat ctttcgaaaa gttccaataa tcctttagga aaaccatact
      481 tagaaaataa tcctatggat caagtagcaa ctatcacaga taaaactacg ttagctgccc
      541 gtgcaaacgc aatcgagaaa aaattatttc tagacgaaga tacacaaaat tatctagatt
      601 atattgccaa tgaatataaa aaaactttga gcaaacgagc tttgatctat tatcaacgag
      661 atgtagaaag agacctggcg atcaatgcat tgattttagg atctggatta agactctccg
      721 aggttgtaaa tattaattta gaggacctat ctttagaaaa gaataatgtg gtagtaacta
      781 gaaaaggaaa taagagagac gcagtaaata tagcagcatt tgcaatggaa tatttagcta
      841 attatctagc tattcggaaa gaacgataca aagtaacaga aaacgaaaaa gctttatttc
      901 tagcaattta tcaaggagaa gccaagcgta tatccggtat tgcaatcgaa cggatggtag
      961 caaaatattc aaaaggattc agagtccaag tcagtccaca taaattacgt catactttgg
     1021 ctactagact ctatcaacag acaaattcat tggtattaac tgcccaacaa ttgggacata
     1081 caagtacaaa tacaacaact ttatatactc atatagacaa tgcagctaca attgatgctt
     1141 tgaacgcttt gtaaatgtag taaaaaatag tataataatg aagcctgtct ctaaaattag
     1201 ataaaagccc aactttaaag gcaagcttca ttatcatttt ta
//
LOCUS       DAGCYL010000291         1214 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.304, whole genome shotgun sequence.
ACCESSION   DAGCYL010000291 DAGCYL010000000
VERSION     DAGCYL010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.304"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..735)
                     /locus_tag="MB216_002774"
     CDS             complement(<1..735)
                     /locus_tag="MB216_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBT4527532.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFL"
     gene            complement(739..1128)
                     /locus_tag="MB216_002775"
     CDS             complement(739..1128)
                     /locus_tag="MB216_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527533.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
BASE COUNT          388 a          264 c          202 g          360 t
ORIGIN      
        1 taaaaaagtg gaagcttctt ccattagtgg tatttgtacc atatgtcgct gtgtttttga
       61 ggaacgaaaa acaatttctt ttccgtaaga cttatgccga tttttttcta caaatcgttc
      121 cgtttgtata taagggattt tttcgtcagc ttgtccatgc caaaagaaca gaggacgtcc
      181 agctaacttt tccggctgca atgacaaatc atagctctta acccatgaaa tcaaatcaaa
      241 atagtctttt ggtacgacat aattatattc cgatgcgcgc cttacgattt cttttccata
      301 ttccaaaggt gcaggggaac ccatcaaaca cacgcccgct actatatgag gatgtttagc
      361 caacaaggcg catgttgtca ttccccccat cgaaaccccg ccaactgcaa gctgatcatg
      421 tagaagacct cttttttcta aatgcttgat aagtgtgtca aattcaaaca gatttccgta
      481 gatactatcc caaaatgtat aggatggaat agaagaaacc ggctgtttgc gctccccatg
      541 attcatcgca tctggcagga tcacacgaat gccctgctgc gcaatttttc ttccttgcgt
      601 caaaactaac tccttacttg tctgccatcc gtgataatag atgacaacgg gaatacgttc
      661 ctctcttaat tctttcagaa caacttctaa cactggaata tgatggacag aacgtttcaa
      721 tataattgga ttcaattgtt actcacctat tttcattaaa tttcctgatc gccatagcaa
      781 ccatgctcca acagttgtct ggaacaggtt cagtgtacca aataatataa atgcaggact
      841 gaatggctcg ggaaataagc acagagaaca aagtcccacc actcctaatg ctagcgaaag
      901 gagtggataa atagaaaaat tcaaaaaact ggcaacgcta ccgaaaatat taccaataaa
      961 cagtatcaaa aagaaaaaaa cgcttaaaat taaaccatct gtcagcgaca tctgaaaaac
     1021 aatgttctct acctcaattg gctgttgaat cgaatagaca cctaagtata tactgagaag
     1081 gagacttagc acgtggctgg cacaaaacac acgattgacc gttttcattt taatcaccta
     1141 acctatcttt ataattctat tataatcatt ttatcatttt tttgtatgta taaagtttca
     1201 atttttcttc actt
//
LOCUS       DAGCYL010000292         1193 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.305, whole genome shotgun sequence.
ACCESSION   DAGCYL010000292 DAGCYL010000000
VERSION     DAGCYL010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.305"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            70..1026
                     /locus_tag="MB216_002776"
     CDS             70..1026
                     /locus_tag="MB216_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527534.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1080..>1193)
                     /locus_tag="MB216_002777"
     CDS             complement(1080..>1193)
                     /locus_tag="MB216_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBT4527535.1"
                     /translation="FISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT          423 a          176 c          202 g          392 t
ORIGIN      
        1 ttgacaggga atcctaaaaa cattcggctt tttttatata atctttttaa ctcaacagag
       61 ataagttttg tggaattgta tacgctacct ataattataa aaacagaaaa aatgttaaaa
      121 gatttaaaac agtattatcc tctgtctgct tatgaaaaaa agaaattatt ccacttttta
      181 gcaatcaacc tgttggctga acaagctgat tctttagata ttgctagcaa tcagtcgcta
      241 tttttttcgg atataggatt ttaccactta aaaggagtat taggagaaaa atccgttatt
      301 tttatcaata agacactctc ttggctttat cttcatgata aattggatca aaaatacttg
      361 agaaaaaatg aagatgaaac aatcgaatgg ttgaatgaac gattcattga aagttttgaa
      421 aatcagttta atctgttgcg agaagagacg agccaattga tccgaagaga gttaaccaaa
      481 atacatttta aacttttgca ttacactatt aataaagctg atgaatttcc acttgatgtt
      541 tcttttttta gagaaaacta tccagcattt tattgctatt tgatagagta tataaatgat
      601 ctaacgtcaa aacgcaaaga gttgcttcaa gataaatttt ttcttttttt cagttattta
      661 ttattattga tcaatcacat acccattcaa ctgatttctg aaccagtaaa gatcatcatc
      721 gatttttctt atggtgctgc ctataatcaa tttatcaaga aaaatttaag cttgtacatt
      781 aatctgaaca cggaagtgat agaggcagga aagcctgagt tcccggatgt tataatcacg
      841 aataggaata atctatatga agaaagtgca tctcaagtag tggtttggct cgatccgcca
      901 agagcagtag actgggggaa tcttacaaag attttgttga ctatccaaga agagaaatac
      961 aaagacagca aaattgacaa gcaaatagac gattttcctg aagaaatcat cgacaggatc
     1021 gaatagtgaa cttcttttag aaagtccgac tgctttttct aaaaatatta tttgtcaaat
     1081 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1141 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaa
//
LOCUS       DAGCYL010000293         1152 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.307, whole genome shotgun sequence.
ACCESSION   DAGCYL010000293 DAGCYL010000000
VERSION     DAGCYL010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.307"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            37..591
                     /locus_tag="MB216_002778"
     CDS             37..591
                     /locus_tag="MB216_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4527536.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            845..>1152
                     /locus_tag="MB216_002779"
     CDS             845..>1152
                     /locus_tag="MB216_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527537.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     "
BASE COUNT          470 a          118 c          212 g          352 t
ORIGIN      
        1 catgaactca gaatattttt aagaagggaa acaatagtga gctataatgt acaaccgtta
       61 aggacaaatg aagagataca agattttcag ttttggctaa gaagaacaag taatccagaa
      121 agagatagtt ttttattttt gtttggaatc aataatgggt tacgtatgtc agatattata
      181 ggattaaaag ttggagacat acgaggtaaa agtaagccta ttattgttga acataaaaca
      241 ggtaagcgaa aaccgatttt tatagataat ttgagagaag agattttatt atatactgaa
      301 ggtaaagaag aaaatgattg gttatttcca agtcggcaac aaggtcgaca tataacaagg
      361 gatcgtgtct atcagatata tgcagatata gcagaaaaat taggaagaga tgatatagga
      421 acacatacat taagaaaaac ttttgggtat cactactaca agaaaactag agacattgct
      481 accttaatgt ttatttttaa tcatagctcg caagcaatta caaaaaggta cattggtata
      541 actgaagatg aaataggggc atcgttacgt ggttttaaat tgggaggcta attgagatat
      601 taggatatat tttttgtgta taatgaaaac ttagaaggtg agattaatga agataactga
      661 attttcggaa gtatttaaaa cgtttagtaa catttacaat agaataccag agatagaaca
      721 aaatgttgta gttgataatg attattatat agagaatgat tttaataaac caagaaaata
      781 tgacaaccta tacaatttta tacacaatag aacaacaata aataacaaca gtaacactcg
      841 agttttgatt aattcagcat attttcatat taaatattta aagaaatatg agtatgaaga
      901 agttgtatta gattatgggc taaatagaca gtcattttta atttatgcaa taaataacgg
      961 aaatttgcca agcaataaaa agaacataat tattgaaaca tatgcctatc tggagggggg
     1021 atctaaaaaa ctaattaagt catgtaatat agagtatcaa ttatcagtag gagaaattaa
     1081 atgtgttttc gaagaagatt tagaacttga cttaaaaaaa atttttgcca gcattcatga
     1141 ttatataggt tt
//
LOCUS       DAGCYL010000294         1147 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.308, whole genome shotgun sequence.
ACCESSION   DAGCYL010000294 DAGCYL010000000
VERSION     DAGCYL010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.308"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            440..1030
                     /locus_tag="MB216_002780"
     CDS             440..1030
                     /locus_tag="MB216_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBT4527538.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT          381 a          179 c          196 g          391 t
ORIGIN      
        1 taacatttag tcacagtaat agcagatatg ctcgcaaaat gagatatgaa tgtacgcaga
       61 atccttgttt tctacggcat tcgctgtttt ttcattggac tagttatata tttctagggt
      121 tgttttcatg tctgagtgcc cgatatgatt tatttttcgt ttctataatt actttttgcc
      181 ttccagtttc ttttaagatt gtactcaagc aagtgggaat tggttctttt gtcatccatg
      241 tatgcaaaat gttataatgc tggtaaacaa aaatgattac ctgtcatttt tatttagttc
      301 gtatttaatt aagttgactg tctgtatact ctgtctaaaa cttaagaatt gttgttttcg
      361 ctgtcgctat ccaaatttaa tgaatgtctg tatgttaaaa ctgttgtcct tgtcatagat
      421 agtaaaggag agcttgaaaa tgaaaactgc gattacgttt ctattagagg aatatgccga
      481 ttgggaaggt gcttatatta ctagcacact aaaccaaaaa gagggatggc aaaacaaaac
      541 tgcttctatc caatcagagg tcacttccat tggaggatta aaaacaaaaa tcgattactt
      601 aatcgaacaa atacctgaaa aagttgacct ttttattatg atcggtggca attcatggga
      661 cataaaaaat catcatttat atcaaataat cgataaattg ttaaaaacag acgtaccagt
      721 agctgctatt tgtggggcag ttgattattt agctcagagc ggattattgc aaggttacaa
      781 acacaccgga aatgcgcaat ttccttggtt agaatcggaa aattatcaga ataaagaaga
      841 ttttatagaa caacaagctg tgaaagatcg taatttgatt actgcgaatg gaacagctcc
      901 tatagaattc acagacttga ttcttcaagc agtccatttt gaaacagaag aaataagaga
      961 aaaaaagatt cgtctataca aacttggatt ctatgattac tataaaaagt acggagatcc
     1021 tttctcctaa tcattctctt caattaaata ctccttatca atagtatgac aagtaatttt
     1081 actgatggtt atttgtcaca ctttttctga catttatcaa agaagataca ctctatttat
     1141 attcata
//
LOCUS       DAGCYL010000295         1145 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.309, whole genome shotgun sequence.
ACCESSION   DAGCYL010000295 DAGCYL010000000
VERSION     DAGCYL010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.309"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>1145)
                     /locus_tag="MB216_002781"
     rRNA            complement(<1..>1145)
                     /locus_tag="MB216_002781"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          237 a          352 c          229 g          327 t
ORIGIN      
        1 cctcttcaga cttactgctt ggacagacat ttccgatcgt ctgcattcct tagcctcctg
       61 cgtccctcca ttgctcaaac aaaaacaacg ggtacaggaa tatcaacctg ttatccatcg
      121 cctacgcctg tcggcctcgg cttaggtccc gactaaccct gggcggacga gccttcccca
      181 ggaaacctta gtcattcggt ggacaggatt ctcacctgtc tttcgctact cataccggca
      241 ttctcacttc taagcgctcc agcagtcctc acgatctgcc ttcaacgccc ttagaacgct
      301 ctcctaccaa tacacctaaa ggtgtactcc acagcttcgg taatatgttt agccccggta
      361 cattttcggc gcagggtcac tcgactagtg agctattacg cactctttaa atggtggctg
      421 cttctaagcc aacatcctag ttgtctgtgc aaccccacat ccttttccac ttaacatata
      481 ttttgggacc ttagctggtg gtctgggctg tttccctttc gactatggat cttatcactc
      541 acagtctgac tcccggatat gaatgaatgg cattcggagt ttatctgaat tcggtaaccc
      601 gagatgggcc cctagtccaa acagtgctct acctccatca ttctcaattc cgaggctagc
      661 cctaaagcta tttcggagag aaccagctat ctccaagttc gtttggaatt tctccgctac
      721 ccacacctca tccccgcact tttcaacgta cgtgggttcg gtcctccagt gcgttttacc
      781 gcaccttcaa cctggacatg ggtagatcac atggtttcgg gtctacgact acatactcaa
      841 acgccctatt cagactcgct ttcgctgcgg ctccgtctct tcaacttaac ctcgcatgca
      901 atcgtaactc gccggttcat tctacaaaag gcacgccatc acccattaac gggctttgac
      961 ttgttgtagg cacacggttt caggttctat ttcactcccc ttccggggtg cttttcacct
     1021 ttccctcacg gtactggttc actatcggtc actagggagt atttagcctt gggagatggt
     1081 cctcccggat tccgacggaa tttctcgtgt tccgccgtac tcaggatcct cctaggtgtc
     1141 ttcca
//
LOCUS       DAGCYL010000296         1143 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.310, whole genome shotgun sequence.
ACCESSION   DAGCYL010000296 DAGCYL010000000
VERSION     DAGCYL010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.310"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            93..1124
                     /locus_tag="MB216_002782"
     CDS             93..1124
                     /locus_tag="MB216_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBT4527539.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTETDQLQNQVLLDNFVEIMK
                     EDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKAEELEKTVVVYEELESYGI
                     DADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEKAIAAKLANEQRETFNELP
                     TVSTENWLE"
BASE COUNT          480 a          169 c          184 g          310 t
ORIGIN      
        1 tgaaagtgtt ttattcaccc ttaatacatg gtatagttca cttgttgaaa atcctgaata
       61 tatacaagtt tctaagaaga aggagaaatt taatgtctac agaaaatcaa tctcaaaaaa
      121 tgataattaa aaattgggga tataaagcaa cgaatgttta tattgaaaaa tattataaat
      181 ttcctagttt tttaattcag aattctaaat actctaaaat gaaagacagc tcaaaaatag
      241 cctatatgct ttttaaaaat gaatttcgta gagccttaca tgaaaaatgg atcgacgatg
      301 atggtctatt atatttggaa tttacacaag cagacctaat ggacaaatta aattgctacc
      361 aaggcaaagt aaaaaccatt ttaaacgagt tagaaacata taatttgatt tcagtcgtaa
      421 aaggagaatt taacccaaaa acgaaaaaaa atgaaaaaaa taaatactat ttacttcaac
      481 ctgaaataag tgccgatgat ctttttattc aaaattcttt cgatgacgat ttaagcgaaa
      541 ctattgataa atcaacctct tctcggaatg cggaaatcgc acgtcgagaa aaaacagctg
      601 aaacccttga taacactgga aatgcggaaa tcgcacaaga ttattatatt aataaatctt
      661 tagacactaa aagacactta aaagacactg aaacagacca attacaaaat caagttttgc
      721 tagataactt cgttgaaatc atgaaagaag acagcataaa cacatttgta ccagaaaagg
      781 tattaagctt gataaaaaca ttttctaaca cttacgaaga agcccaacaa accgtcacca
      841 caatacacaa cgcaaaacat aaagccgagg aacttgagaa gacggtagtt gtgtatgaag
      901 aacttgaatc ttacggaatt gatgcggata aaggattata tatgacgttg ctgaaagctt
      961 atcaaaaaca aaagactgag aaagtaaata atttgcagaa tttgattttt gtgtatgtaa
     1021 aaaactggtt tattgaaaaa gctattgcgg caaagcttgc aaacgaacaa agagaaacat
     1081 ttaacgaatt accaacagtt agtacggaaa attggctaga gtagttgata aaaagcgaac
     1141 act
//
LOCUS       DAGCYL010000297         1132 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.311, whole genome shotgun sequence.
ACCESSION   DAGCYL010000297 DAGCYL010000000
VERSION     DAGCYL010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.311"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            43..249
                     /locus_tag="MB216_002783"
     CDS             43..249
                     /locus_tag="MB216_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527540.1"
                     /translation="MKQKVVGEVISKLENCVVICVDEYHDVDRNEILEKCGKVVAKYT
                     HVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(611..850)
                     /locus_tag="MB216_002784"
     CDS             complement(611..850)
                     /locus_tag="MB216_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527541.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          394 a          162 c          210 g          366 t
ORIGIN      
        1 actatcattt taggaaaaaa atatatttgc caacctattg gcttgaaaca aaaagtggtt
       61 ggagaagtaa tcagtaaatt agaaaattgt gttgtgatct gcgtagacga atatcatgac
      121 gtcgatcgca atgaaatatt agaaaaatgc ggaaaagtag tggcaaaata tactcacgtt
      181 tatgagttgg aaacaacaga atatctatat aactatagca acaaatatga acatacttta
      241 gtttcataaa gatttaaata aagtgccttc aaaagcgaga agaccaagct tttgaaggca
      301 ctttatttag ttggcagtga ttgttcatga tttttgattt attcaaaagg ttcgcctaga
      361 gaatctgtct ctgtaacgag gcctggtgga gaaactcgat aaaaacctat actgccagaa
      421 ctgtcctatt ttcggataga caaactagta gttttaaaaa gatcatccga tccaatcatt
      481 tcaaaaaatg caaaactgat atcaggatca tttagctcag gcttgttatc gtaaagcatt
      541 tgtaaaggtt gcatttagtt aggagtaaac gcgaaactat catcattatt tgctgaagaa
      601 gatgaagaag ctaattcaga gtttgtttga aataaaggag acgtgctgac agtttcttta
      661 ccctgttttt tgcagaggta gaaactttta tatcggaagt ttctaccgga gaatttttct
      721 catttgtaga aattgttgaa tcgatactat tcgaaatttg cgtatctgaa ggtatgttct
      781 ttgatgcaga agtcccacaa gcagtaagta acaataaatt tgataaactg ataaacagaa
      841 ttcttttcat tatgtacaca ctccttatag tatcgtttag catacaccta tgctcagtat
      901 taggcaaaat caatgcaaaa ctatcaagtc ttttatagta tctattagtc ttagtaatac
      961 atatatggag agggtgatat cccagttcaa accagataca ttggttacca tctgtattaa
     1021 cgattatgcc tctaaacgct ttttttatga aaaaatttat aagtgaaaac gtaggtatga
     1081 tattatctag aggtaatttt tttgggtatt taaatagttg ctaggaggat ag
//
LOCUS       DAGCYL010000298         1117 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.312, whole genome shotgun sequence.
ACCESSION   DAGCYL010000298 DAGCYL010000000
VERSION     DAGCYL010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.312"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            96..506
                     /gene="spx"
                     /locus_tag="MB216_002785"
     CDS             96..506
                     /gene="spx"
                     /locus_tag="MB216_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBT4527542.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     TLAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            520..876
                     /locus_tag="MB216_002786"
     CDS             520..876
                     /locus_tag="MB216_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4527543.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVEKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          385 a          164 c          210 g          358 t
ORIGIN      
        1 ttattttgat ttttttttat attttattag ctaataattg gtattttaaa agaaattact
       61 gtagaattgg attataatta aaaaggagtg agaaaatgat aaaagtatat acttcgccta
      121 gctgtacttc ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt gaatttgaag
      181 agaagaatat ttttgccgaa ccgttaacag aaagtgagat caaaaagacc ttggcagcga
      241 ctgaaggagg agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca
      301 tagattttaa cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat
      361 tattgagacg acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg
      421 agatccggtc atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac
      481 aaaacaataa catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga
      541 tcagcgcact gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct
      601 tatcaatttg ttgacttaga agaacgcgat ctaagtacag aagaagcaga agaattatgc
      661 agttttgaag aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac
      721 attgcgattg acctttcctg tgtagaaaaa aaacaactga tttttttatg cacacaacat
      781 accaacctcc tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat
      841 gagcagttga tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca
      901 ctgagcattc catacagttg taacagtcga acacttagca tcaagcgtgt tatgtgttct
      961 ttttttgact ttatttttct actatagaga catgctcaat ttatgtggtc aaaattcaaa
     1021 tctaggtggg gatatagagt taaaaatata ttgacatgtg taaatttaca tgatatattc
     1081 atttctgttg ttttaagtag caaagcaata aaatagt
//
LOCUS       DAGCYL010000299         1107 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.313, whole genome shotgun sequence.
ACCESSION   DAGCYL010000299 DAGCYL010000000
VERSION     DAGCYL010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.313"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            46..261
                     /locus_tag="MB216_002787"
     CDS             46..261
                     /locus_tag="MB216_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4527544.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            298..660
                     /locus_tag="MB216_002788"
     CDS             298..660
                     /locus_tag="MB216_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBT4527545.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            706..1032
                     /locus_tag="MB216_002789"
     CDS             706..1032
                     /locus_tag="MB216_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527546.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT          418 a          132 c          242 g          315 t
ORIGIN      
        1 aaatatatca tattgttgta tataataaaa caggagggga gaatcatgaa aaaagtaagt
       61 aatctcagcc gatacagaaa agaaaaagga ctttctcaaa cggagttagc aaaaaaaatg
      121 aatgttacac agcaatgtgt aagttcgtgg caaacaggga gaacgattcc taaaccctat
      181 caaatgaaga tgctttctga gatattaagt gtaccaatga atgagctgtt ttctgatgta
      241 tttaataaag taaacagtta actaataagc aattgaatga ttttaggagg agaagacatg
      301 tattgtagaa aatgtggcaa agaaataaca gatgaaagta agtattgcca gttttgtgga
      361 gcactacaag aaacagatga gacaaccgga acagacacag taaagaaaag tactgagcag
      421 acaattaatg aaatgaagga acaaatcgta catggacaag agacagggaa gctgtatttg
      481 attattggtt ggatttctat ggtagtatcg ttagcattta ttcctgttgt atttggtgct
      541 gttgctgtta ttatgggcta cttatatcga gaaaaagatg agaaaatggg tacgattttg
      601 atgatagcag gggtagctgg agcaatcttt ggtgtacttt tagggatgtc aatgtactga
      661 taagagggat aagaacaaat tattttggag ataggagata aaaagttgaa taaagtaaat
      721 atgaacaaga agtttgtcat tataggtatt gttgtcttgg ctattatagg tattattttt
      781 tcaaaaatga caggtacatc tggattagag aagtcatcaa aaccattggt aacacagatt
      841 ttacaagaac agtacaactt aaatcgatca tgtgatgatg tgacgattac tcatgaaaat
      901 ggagataaca cataccgggc taaagctata ttagacaatg gaagtgctat caacataaat
      961 attgagtact atccaaagaa agaccgtata tatgtggaga taccatatgc tgaggtgtta
     1021 atgttaaatt gatttaaatg tattatgcat accgtccaaa caggaactcc caaaggtgta
     1081 aattcactag ggagttttta tagtttc
//
LOCUS       DAGCYL010000300         1107 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.314, whole genome shotgun sequence.
ACCESSION   DAGCYL010000300 DAGCYL010000000
VERSION     DAGCYL010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.314"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            191..445
                     /locus_tag="MB216_002790"
     CDS             191..445
                     /locus_tag="MB216_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBT4527547.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="MB216_002791"
     CDS             446..763
                     /locus_tag="MB216_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBT4527548.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          391 a          173 c          220 g          323 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacct
//
LOCUS       DAGCYL010000301         1089 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.315, whole genome shotgun sequence.
ACCESSION   DAGCYL010000301 DAGCYL010000000
VERSION     DAGCYL010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.315"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..279
                     /locus_tag="MB216_002792"
     CDS             <1..279
                     /locus_tag="MB216_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBT4527549.1"
                     /translation="KRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLS
                     QGGKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            622..878
                     /locus_tag="MB216_002793"
                     /pseudo
     CDS             622..878
                     /locus_tag="MB216_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
BASE COUNT          379 a          164 c          198 g          348 t
ORIGIN      
        1 aaacgtaatc ccttcgtatc tgaatatgtt aaacgacttg caaaaggtat ttgtcaactt
       61 tgccaagaaa aagggccatt tataaaagac ggtgtacctt atttacattg tcatcatata
      121 gaatatttgt cgcaaggtgg aaaagatgtg attgaaaact gtatcgcgtt atgtcccaat
      181 tgtcatgcta gaattcatga acttgagtta cctgacgata aagaaaaatt gctgaagatc
      241 gttgaaaaaa gagaaaatag cttttctaaa ataccgtaat ttatttttat aggacaaata
      301 agctaatgtg agctatgcct tctatataag actaagttct attataagta acgaagtaat
      361 tctcgttcct tcctgtagta atatttttta aataacctat aggcttcttc aaaagtacat
      421 cctccagtaa attctaagta ctggaggatt ctttttctag tgttgcttta ctcatgaaaa
      481 agtagttggc tacttcatca aaatttctga acttttttaa tgtataataa agagctcgat
      541 catttactac aaggatgcta gctcctacat tttcacagtt ttttagcaag taaatttttc
      601 taaaatcaca tcaggaaatg catgagattc tttaagaaag gcaattatag ggctatgatt
      661 acggttcgtg taagtgacgc caaaaaagaa tggctttctt atatggctga attttatggg
      721 attactttat ctgaacttct aaaaaactat tcgatggaac aactagaaga tgaatacaat
      781 cgtcaaacgg ctgaaattgc ccataaacat tggataagaa gtgggaaaac aacagtattc
      841 atggaagaat ccttaatgaa tttggtgggc tggaatgacc tgccgtttaa aattcacgcc
      901 cgaaattcag aagcagctct gattggcaaa tacgtgtatt ggtagagata gaagataatc
      961 aattcgttgt taccgctatt caagtagggc accgaagaaa tgtctatgat taagagactg
     1021 tttattttta caaaaaaata ataactggaa tctagttaaa actcttgaag tgagagattt
     1081 gactagatt
//
LOCUS       DAGCYL010000302         1084 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.316, whole genome shotgun sequence.
ACCESSION   DAGCYL010000302 DAGCYL010000000
VERSION     DAGCYL010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.316"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            195..>1084
                     /gene="aph(2'')"
                     /locus_tag="MB216_002794"
     CDS             195..>1084
                     /gene="aph(2'')"
                     /locus_tag="MB216_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021401024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ie/IVa family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HBT4527550.1"
                     /translation="MTTYTFDQVEEAIEQLYPDFTINTIEISGEGNDCIAYEINGNFI
                     FKFPKHSRASINLLNEVTVLKTIHNELSLPIPEVVFTGMPSEMCQMSFAGFTKIKGVP
                     LTPLLLKNLPKQSQDQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKL
                     LSRELKGHQMKKVDDFYRDILDNEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDF
                     GDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYMMKEKYWSFEKI
                     IYGKEYGYMDWYEEGLNEIR"
BASE COUNT          410 a          157 c          204 g          313 t
ORIGIN      
        1 aaatggccat tagaagatag gaacttagca gatactgcac tttggctaaa tgcagaattt
       61 atgaaaaatg cagcagagat aggttatgca agatttttat atggaaatag catgaaacaa
      121 aaatgaatta actataattg ctaaattctt atttacagtc aatcagattt ttgattaaag
      181 ggagtagaga agacatgaca acttatactt tcgaccaggt agaagaggca atagagcagt
      241 tatatcctga ttttactatc aatacaatag agatttcagg agaaggcaat gactgtattg
      301 catatgaaat aaacgggaat tttattttta aatttccaaa gcattcaaga gcttcgatta
      361 atctcttgaa tgaagtaacc gtactcaaaa caatccacaa tgaattatca ctacccattc
      421 ccgaggtggt ttttacagga atgccatcag aaatgtgcca aatgtctttc gcaggtttta
      481 caaaaattaa aggagtacct ttgacacctc ttctactcaa aaatctgccg aagcaatctc
      541 aagatcaggc agctaaggac ctggcccgat ttttaagtga acttcacagc ataaatatct
      601 ctggattcaa aagtaatctg gtattagatt ttcgagagaa gataaatgaa gataataaaa
      661 aaatcaaaaa gttactatcc agggaattaa agggtcacca gatgaagaaa gtggatgatt
      721 tttacaggga tattctagac aacgaaatct acttcaaata ctatccttgt cttattcata
      781 acgatttcag cagcgatcat attttatttg ataccgaaaa aaataccatt tgtggaataa
      841 tcgattttgg agatgcagct atttctgatc ccgacaatga ttttataagt ttgatggaag
      901 atgatgaaga gtacggcatg gaattcgtat caaaaatatt gaaccattac aaacataagg
      961 atataccgac agttttggaa aaatatatga tgaaagaaaa atactggtcg ttcgaaaaga
     1021 ttatctatgg aaaggaatat ggttatatgg attggtatga agagggatta aatgaaatca
     1081 gaag
//
LOCUS       DAGCYL010000303         1068 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.317, whole genome shotgun sequence.
ACCESSION   DAGCYL010000303 DAGCYL010000000
VERSION     DAGCYL010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.317"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(309..382)
                     /locus_tag="MB216_002795"
     tRNA            complement(309..382)
                     /locus_tag="MB216_002795"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(346..348),aa:Ile,seq:cat)
     gene            complement(571..>1068)
                     /locus_tag="MB216_002796"
     CDS             complement(571..>1068)
                     /locus_tag="MB216_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBT4527551.1"
                     /translation="GYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT          333 a          203 c          185 g          347 t
ORIGIN      
        1 atgcctttta atagcttcaa atataaaaga gtttaaagcg taacacactt atggggaagt
       61 ggcttggggt gcgctttaaa ctcttcttta ttatcatctc acaatttaac ccaaatgtct
      121 ttctatttaa aaatcaaagt aaaacttttc aaatatacag aaatataact atgtgaaaca
      181 tcctttcatt aatccataaa aggatacata aaaaagccac tcatttgagt ggcaatgaag
      241 aaaagcttta gcttgtataa tactcttcaa aaaattctaa cacagaacga ttcaaatggc
      301 tacgttaatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
      361 tgaccaactg agctaagggt actggttgtt gccacataaa gccataaaca atcaaccagt
      421 agaatgtgtg gcaacaaacc tgttatcgca tagcttggag tgtgactatt atgggtgata
      481 gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg tcaatatagt
      541 tatgtaatgc tcctcaacga ggagctattt ttatcgttta ggaatattta aataccaacg
      601 tttgtcatgg aaatcttgcg caccgccttt agtgtttccc tctggatcat tcgttgcccg
      661 catcatgaca tagactttct tattagggaa gttacgcatg ttaaaagata catgatagcc
      721 aacatttcca gaagtattat aagcttgatt tacatctgat ctataaattc catcagctct
      781 tactcgagct aattctttcc cagtattgta atccataatg aagatatact cgtatttata
      841 gttagcaatg tgccatccag ctacatgcaa atttgcattt tcgatttctc caaactgatc
      901 aatgtgggcg taattcgttc catctgtcag tgtaggatta gctgcaccag ctcgtgttgg
      961 atcaatgaca ggcttgtttt cagaagttgt tggattttca tcggtaaatc catgagctaa
     1021 atcatatgct aatttttctt tacttacgcc catttcagaa agataacc
//
LOCUS       DAGCYL010000304         1061 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.318, whole genome shotgun sequence.
ACCESSION   DAGCYL010000304 DAGCYL010000000
VERSION     DAGCYL010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.318"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..956
                     /locus_tag="MB216_002797"
     CDS             <1..956
                     /locus_tag="MB216_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HBT4527552.1"
                     /translation="GTLELKVPRTRDGHFSPTVFERYQRNEKALMASMLEMYVSGVST
                     RKVSTIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVRE
                     ENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKERGLQGTELVISD
                     AHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAR
                     EAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          371 a          199 c          185 g          306 t
ORIGIN      
        1 taggcacgct agaattaaaa gtacccagaa cacgtgatgg ccatttttca cccacagtgt
       61 ttgaacgtta tcaacgaaac gaaaaagccc tcatggcttc aatgttggaa atgtatgtat
      121 caggcgtttc aactcgtaaa gtatcaacaa ttgtggaaga actttgtggt aaatccgtct
      181 ctaagtcctt cgtttctagc ttaacagaac agctagaacc tatggttaac gagtggcaga
      241 atcgtttatt atcagaaaaa aattatcctt acttaatgac cgatgtactc tatataaaag
      301 tacgagaaga aaatcgagta ctctcaaaaa gctgtcatat agcgattgga ataaccaaag
      361 atggcgaccg tgaaattatc ggcttcatga ttcaaagtgg cgaaagcgaa gagacctgga
      421 caacattttt tgaataccta aaagaacgcg gtttacaagg tacggaactc gttatttctg
      481 atgcgcacaa aggattagtc tctgccatta gaaaatcctt caccaacgta agttggcaaa
      541 gatgccaagt tcacttccta agaaatatct ttaccaccat tcctaaaaaa aattcaaaat
      601 ctttcagaga agctgttaaa ggaattttta agttcacaga tattaactta gcgcgtgagg
      661 ctaaaaatcg attgattcat gattatatcg atcaaccaaa atattcaaaa gcttgcgcat
      721 cattggatga tggattcgaa gacgcctttc aatataccgt acaaggaaat tcccacaatc
      781 gactaaagag taccaatcta attgaacgac tgaatcaaga agtacgcaga agagaaaaga
      841 ttattcgcat cttccccaat caaacatcag ccaatcgctt aattggagcc gttcttatgg
      901 acctacatga tgaatggatt tattcttcaa gaaaatacat caattttgat aagtagaaat
      961 ggtaaaaaca ttgtatagca ttttacacag gagtctggac ttgactcact tcctttatta
     1021 tttttcattt ttttaaacta tttaatacta atgtctttta t
//
LOCUS       DAGCYL010000305         1060 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.319, whole genome shotgun sequence.
ACCESSION   DAGCYL010000305 DAGCYL010000000
VERSION     DAGCYL010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.319"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(61..279)
                     /locus_tag="MB216_002798"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="MB216_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="MB216_002799"
     CDS             complement(545..739)
                     /locus_tag="MB216_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527553.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="MB216_002800"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="MB216_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          369 a          183 c          154 g          354 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc
//
LOCUS       DAGCYL010000306         1044 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.320, whole genome shotgun sequence.
ACCESSION   DAGCYL010000306 DAGCYL010000000
VERSION     DAGCYL010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.320"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..174)
                     /locus_tag="MB216_002801"
     CDS             complement(<1..174)
                     /locus_tag="MB216_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527554.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEET"
     gene            complement(276..629)
                     /gene="tnpB"
                     /locus_tag="MB216_002802"
     CDS             complement(276..629)
                     /gene="tnpB"
                     /locus_tag="MB216_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBT4527555.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(619..813)
                     /locus_tag="MB216_002803"
     CDS             complement(619..813)
                     /locus_tag="MB216_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527556.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          301 a          211 c          181 g          351 t
ORIGIN      
        1 cgtttcctca aagagagtct ctttagagcg tccaaataat ttgttggtta aataattgac
       61 cttttcattc aatagttgga tttcagttgt aagattctga atggtttgtt gttgtgcttg
      121 attggtttta tgggcttctt gaagcaactg aagcaacgta tctgtagttt ccaaaatttt
      181 tcaccacctt gaatcgaact gattttgtta cagaatacca caagaaacgc acaagaaata
      241 ggggttcaac acattttttg tgttaacccc ccagcttaaa aaactccttt ttttcctggc
      301 aaaatagctc gaggttgatc gatacttaat ccttctaaca accatcgaac ctgttggttc
      361 gttaaggttc gaacttcgtc tttcgtccga ggccatttta acttgccgtt ttctatccgc
      421 ttataacaga gaagaaaacc atcaccgtcc caatagagaa gtttgaaacg gtcttgacgg
      481 ttcccgcaaa ataaaaaaac ggcatcttcg taaagctcga gttgatactc ttgttgaata
      541 agactggcaa gaccatcaat tccttttcgc aaatctgttt ttccgcacac aatataaata
      601 tgtttggttt gaagtaaatc ataaagcatt ggacaacagt tctttcaaaa tagcttgaag
      661 aatataattg ttaataccgt tatagatcaa aacttcagaa ccattctctc ttttgatgcg
      721 catcgctaac ttggcagccg cgggtttacc tttttctggt gcactctttt cgtttcctaa
      781 ttggacagga acaatctcat ttaatttata catagaaaac ctccagactt cttgatagct
      841 caagtatatc ttgaggtgca tttgttagta aggtactgtt ctatggggcg cttaccttgt
      901 gtcttcctaa aaaggtcttt tcctgtttct atgtaaaaca aaaagagctg caatcctcat
      961 ttaggactac aactcttctt ttatttatat gcccatcgat cgcgtaaaat gatcgggatt
     1021 tctaacatac atgctagcaa taac
//
LOCUS       DAGCYL010000307         1038 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.321, whole genome shotgun sequence.
ACCESSION   DAGCYL010000307 DAGCYL010000000
VERSION     DAGCYL010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.321"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            1..797
                     /locus_tag="MB216_002804"
                     /pseudo
     CDS             1..797
                     /locus_tag="MB216_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            complement(914..>1038)
                     /locus_tag="MB216_002805"
     CDS             complement(914..>1038)
                     /locus_tag="MB216_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBT4527557.1"
                     /translation="AFERKQIHSVLSKNPYVTTHSEGDEPYRYLVVEPSDKTIL"
BASE COUNT          366 a          186 c          178 g          308 t
ORIGIN      
        1 actacacgcc ccaaaccatg tccaacatga ctaaagtgct gactgaagaa gtaaatgcat
       61 ttaaagttag aactctaaat gataagtatg tcgctatttt catggatgct acttatattc
      121 ctttaaaacg gcaaaccgtc tccaaagaag caatctatat tgccattggt atacgagaag
      181 acggcactaa agaagtacta agttatgcga ttgctccgac tgaatcgacc tacgtttgga
      241 atgagttgtt acaggacatt tactccagag gggttcatga tgtcttactg tttattaccg
      301 atggcttaaa aggcatgaaa gatacgattc accagattta tcctaaagct aaataccaac
      361 attgctgtgt tcacgtttcc cgtaatattg ctcataaagt acgtgtcaaa gatcgaaaag
      421 aaatctgtga tgattttaag gctgtttatc aagctagctc aaaggaagag gcaaatacct
      481 ttttagggag tatgattgag aagtagcaaa aaacttatcc taaagtgacg cagtcactga
      541 taaaaaatca agatttattg actttttatg agtttccgcc tggtatccgc cgaagcattt
      601 actcaactaa tttaatcgaa tctttcaaga aacaaatcaa gaaatacagc cgcagaaaag
      661 agcagtttca aaatgaagaa tcactagagc gtttcctagt atccatcttt gatacataca
      721 atcaaaaatt cttaaataga agccataaag gcttccaaca agtgacggat acattagctt
      781 caatgtttac tgagtaaccc attattttgc agaaggacga gttatttaca caaaattatt
      841 gacgctccca ataaaaaaac aaacaaagta aaataatcta ctttgtttgt ttttatttta
      901 attaatattg aatttataaa attgtcttat cgcttggttc cacaaccaag tagcgataag
      961 gctcatcccc ttcagaatga gtggtaacat aaggattttt acttaaaaca gaatgaattt
     1021 gttttctttc aaaagcgg
//
LOCUS       DAGCYL010000308          994 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.322, whole genome shotgun sequence.
ACCESSION   DAGCYL010000308 DAGCYL010000000
VERSION     DAGCYL010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.322"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..283)
                     /locus_tag="MB216_002806"
     CDS             complement(<1..283)
                     /locus_tag="MB216_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527558.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVD"
     gene            complement(532..732)
                     /locus_tag="MB216_002807"
     CDS             complement(532..732)
                     /locus_tag="MB216_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4527559.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          355 a          159 c          167 g          313 t
ORIGIN      
        1 gatcaacgaa agtatacttt atatagtcat aacgccgatg atcgctacgt gcgtccggca
       61 cgtcagtcac gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag
      121 ggattttaaa gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg
      181 ctaaaaagaa aacttctgaa ttccatactt tataaaaatc tttgataaat ctattcgaag
      241 ggtcaaattt aaaccataat tgtgtttttc cttccatcag cataaaagtt cacctcaaaa
      301 agagtatacg aacgaatgtt ctttttgtga aggcggaatt taatatactg aataatagaa
      361 aacaagtact tgtgccaaat tatgtgccaa aaaaattcga attcaacaaa atgtaaacaa
      421 atagaaaacc ttctatatta agatttcctg caataacaaa cacaacagaa gacgtgtaat
      481 agttagtcag gaacgtacaa ataacccctg tatcctttgc ggtacagggg gttattttgt
      541 ataagtagac attgtagttt ctaaactgct atgtcctaaa cgtttcgata cgctaagtat
      601 atttactcct tgataaagta aaatagatgc atgcgtatgt cttagtccat caataaacag
      661 tcgaagagaa aatagggaaa aattgattac taaagaaaaa cttactaggt cgacggtgta
      721 gttgattttc atacttcaat agattcgaat taaacgattt cgattggctt ataccagtcc
      781 tatccaacga aagatatatc gttcttaact ctagcggtat aacttgaagc tttacgtaaa
      841 gccaatggga atgaactgga gatgactttt tattattttt agtaatagct gatgggttaa
      901 tacttacaac tttccttttc aaagtaccat ataaaaaaac ggtggggttg tttagcgaaa
      961 taaaggatct aagccaatag cttagatcct ttat
//
LOCUS       DAGCYL010000309          980 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.323, whole genome shotgun sequence.
ACCESSION   DAGCYL010000309 DAGCYL010000000
VERSION     DAGCYL010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.323"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(128..289)
                     /locus_tag="MB216_002808"
     CDS             complement(128..289)
                     /locus_tag="MB216_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527560.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMKYVADEANRK
                     QRKLVGLDK"
     gene            complement(286..957)
                     /locus_tag="MB216_002809"
     CDS             complement(286..957)
                     /locus_tag="MB216_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBT4527561.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
BASE COUNT          285 a          187 c          139 g          369 t
ORIGIN      
        1 aagtaaacct aataaaaaac acaactaatt tttagctgtg ttttttaagt tacaataatt
       61 atctatttaa tagtcttaat tcattaaaac aaaaaactca actttacaag gatatggttt
      121 aaaaaattta tttatccaac ccaactagtt ttcgctgctt acgatttgct tcatctgcta
      181 cgtacttcat tactgtttta aattctttcg ctgaggcgtt ttctgataaa tcagcgatac
      241 ttttattata cttcttcacg atttgttgta cttgttcttt ctcagtcatg taattcctcc
      301 tccaactctt ttgaagtata ttgtattggg tagtactaat tgaatattat ctgctgcata
      361 atgcacttca gaaatgtcgt tttgataatc cttaatcatt atgtgaagga ctacttcagg
      421 atgtctattt ttgacttttt caatattctc tctggactta aaaaacgcat tgataaacat
      481 ttcttttggc acatgacgtc cttcagctag ttcacgtttt cgagtgaaat cccaggcaac
      541 tttgggatct tggtaaacat agaaaatcgt aacgcgaaag ttatttttaa gggctcgaat
      601 aatatttttc tctgcactaa ggatagcaaa agtatcctct agtatgaaag aatagccatg
      661 ttccagtaca tattttaatg attgttcaac taaccaagaa gaagctttct gaaaaacact
      721 tgagttcttt ccattgtatt ctggaaattg tgctctaaaa tagtccgcat caatcacaac
      781 atggttgtca taattttcag ctaaacctag tgcaacctct gttttacctg ctcctgggct
      841 tccagccatg aataccgcat ctttttcatc atcagggatt tttcccttga tgattgtttc
      901 aataaattta tctttattga ccctagcgta atctaaatac ttgtctttag gttccaagat
      961 tgcaccttct ttccttcttc
//
LOCUS       DAGCYL010000310          958 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.324, whole genome shotgun sequence.
ACCESSION   DAGCYL010000310 DAGCYL010000000
VERSION     DAGCYL010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.324"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(209..757)
                     /gene="aac(6')"
                     /locus_tag="MB216_002810"
     CDS             complement(209..757)
                     /gene="aac(6')"
                     /locus_tag="MB216_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBT4527562.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          281 a          203 c          142 g          332 t
ORIGIN      
        1 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta
       61 agcgactttt tcacaacctc tggtacacgg tgttccaaaa gtagcttctt cggaaataag
      121 ttggaatttc acaaaaattc aagaagtaat tttcgtaaat tccttcttat ttcttgaagc
      181 tgattacttc tgtcacaacc tctttttatt attgagaatt tggtcgagga ataatcgttt
      241 ttgccatcca aatatccggt ttgtcccagc catttgcatt tggtaagaca cctacgattt
      301 tataacctaa tttttcatag aattcatacg gatgttcacg aaggttctgg atggaagcca
      361 ctttatcaaa tgtatgctca tacaggtccg tttgacttaa cgttgttcca tggtctaaat
      421 catccgtacc taaataaatc gtgattcctc ctctggaagc tacttctttt tctaagtaat
      481 tgactaatcg agtacctatt tggttctttc gtcgggagct ttctacaact aatggatgca
      541 attcccaacc tgtgataccg tattgaggga ttgcaccaat aaatcctact aactcatctt
      601 ggtctaccgc tgctaccgcg attcgttctg gattcatcat ttcttctact tcttctgccg
      661 agctgtctcc atattcttcc ggccaagtca gtctcagtaa atcagaaagc tgatctttca
      721 atactggatt attacggtca aattcactga ttatcatacg tcttccccct tgttcttgct
      781 tattctaaca gtccttttga aaaaacgaaa tgttctcgct caaaaatctt tagctaccaa
      841 tcagtatctc tattctttca ttatttatct tacttataaa ataaaaaaat tatacagaaa
      901 cactttattc aaaataggaa agatcaggaa agccatcaat tggaggaaaa aactctgt
//
LOCUS       DAGCYL010000311          954 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.325, whole genome shotgun sequence.
ACCESSION   DAGCYL010000311 DAGCYL010000000
VERSION     DAGCYL010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.325"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..408
                     /locus_tag="MB216_002811"
     CDS             <1..408
                     /locus_tag="MB216_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBT4527563.1"
                     /translation="MLPVVMISWLLVSVYTSNELLSSLEKIHLPKKIMLSITVVIRFF
                     PTYRSEIRMIKESLKMRNIRLSFFQPLQYLEFLLVPVLIRATMIAEEMTANAITKGIE
                     SPVKRSSYYKTNMSLLDYIILFSVTIFFLSILI"
     gene            419..>954
                     /locus_tag="MB216_002812"
     CDS             419..>954
                     /locus_tag="MB216_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4527564.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATK"
BASE COUNT          331 a          132 c          163 g          328 t
ORIGIN      
        1 atgttgccgg tcgtaatgat ctcatggttg ttggttagtg tctatactag caatgaatta
       61 ttatcctctt tagaaaaaat acatttaccg aaaaaaataa tgttaagtat tacagtagtg
      121 atcagattct ttccgactta tcgttctgaa ataagaatga ttaaagaatc tttaaaaatg
      181 agaaacattc gcttgtcttt ttttcagcct ctgcaatatt tggaatttct gttagttccc
      241 gttcttatac gtgcaactat gattgctgaa gaaatgaccg ctaatgcaat aacaaagggg
      301 atagaatctc ctgtgaaaag aagttcttac tataaaacaa acatgagctt attagactat
      361 atcatcctgt tttccgttac tatttttttc ttaagtattt tgatttgagg tgaaagaatt
      421 gtttgaattt aacaatgttt ctttttcata taaggatgtt aaggaacaag cgcttgttga
      481 ccttaatttt tcaataaaaa aaggtagtct tacattgatt actggttctt ctgggagtgg
      541 gaaatccaca ctattaaagt taatgaatgg acttattcca agactttatg acggaaaatt
      601 ggttggggaa attttatttg aaaataactc gctacaatcc ttaacacaag aaagtatatc
      661 aaaaaatatt ggctacgtaa gtcaagatcc tagaggacaa ttctttacaa caaatacaac
      721 gagtgagtta gtattttcga tggaaaactt tggatttaca aaagagcata tggataaaag
      781 aatcaatgaa ttagctgaat tattagatct ttcaaatata ttagataaaa acattttttc
      841 gctatcaagt ggtgaacggc agaaaatttc tattggatgt agtttatcgc taaactctaa
      901 attaattata ttggatgaac cgtcttcaaa tttggacttc aaagcgacta agaa
//
LOCUS       DAGCYL010000312          930 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.327, whole genome shotgun sequence.
ACCESSION   DAGCYL010000312 DAGCYL010000000
VERSION     DAGCYL010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.327"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(358..531)
                     /locus_tag="MB216_002813"
     CDS             complement(358..531)
                     /locus_tag="MB216_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527565.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(534..>930)
                     /locus_tag="MB216_002814"
     CDS             complement(534..>930)
                     /locus_tag="MB216_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527566.1"
                     /translation="RLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKASK
                     IAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHTS
                     MKPCIFDWQWLIDAVLDVKCSKYNITLGC"
BASE COUNT          338 a          150 c          102 g          340 t
ORIGIN      
        1 ccgtagaaat atattggttt tgccttcaga tcaatactgg atcatattaa tctgaatata
       61 atatatgaac taataaatag cttaacctcg ctattttgtt ttttctgatc actatctata
      121 cagcattagt aaatagaatt ttaaaaataa aaaaattaag acaaaaaagt atcaacataa
      181 acatatacat atacacgttt atgttgattc acttaatttg tcctttaaaa agcacttatt
      241 ttaatataat gttattttat aatcttatta caagctgatt gttaggcgtt gtcctttcat
      301 tacatttatt aacggttatt ttattgttta atttattaat tatttttgat ctaaaactta
      361 gcttaacaaa taaataatta accaaccgcc agataaaaat gcgatcattg ctaatacata
      421 agcaaagata aaaatatgtt cgatcagctt aaaccctgat gcgattaagc agaccaagta
      481 aaatagaaat tctcttacag ataatggtaa ttttctaact agcatactca taattaacac
      541 cccaaagtaa tattgtattt tgaacatttt acatccaata cagcatcaat caaccattgc
      601 caatcaaaaa tacaaggctt cattgaagta tgtttttcag atgaaaagat ataatataat
      661 ttcttcggaa taatattttc agttgttggg atcaagctaa atctaatacc gtctaactca
      721 tagctaccag ctttctctcc attaaaaatt tccttagcac tttcttctaa agaatcaaaa
      781 tatttttcga cagcaatctt tgaagctttt actattgaag catttccctt ttcgataaaa
      841 tctgcttttg ttttaccgac aataagtaat tcttgatcag taaaatcttc taaaataatg
      901 gtagtgtaat tcttatttct tctcaatcgc
//
LOCUS       DAGCYL010000313          914 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.328, whole genome shotgun sequence.
ACCESSION   DAGCYL010000313 DAGCYL010000000
VERSION     DAGCYL010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.328"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>914
                     /locus_tag="MB216_002815"
     CDS             <1..>914
                     /locus_tag="MB216_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4527567.1"
                     /translation="SVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDRSGEI
                     AEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELLYFGT
                     QTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSANNKLF
                     NRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSFNPRI
                     FDSFEVHEDILVFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRSINIL
                     QRFEKEVKIDPDFSPKLKKLVKFLLINK"
BASE COUNT          358 a          117 c          156 g          283 t
ORIGIN      
        1 tagtgttata gtacccgcat ataacgtaga aaactattta gaaaactgct tagaatctat
       61 ttccaatcag acacattcaa atattgaaat aatagtagtc gatgatggtt ccccggatcg
      121 aagtggggaa attgcagaag aatataaaaa gaaagatatg cgattaaaag tgatacatca
      181 agtgaatggt ggtttaagtg cggctaggaa ttctggtctt gattctgcaa caggagattt
      241 tgtgtgcttt attgacagcg atgattggat agaaccagac tatcttgagt tactatattt
      301 tggtacgcaa acttatggag cagatatctc ggtgctaaaa ataaaaaaga tttcaagttt
      361 agaacaacta aaagaacata caattgctga gagaaaatgg gaaatatttg atagagaaca
      421 agcaatgaaa aacttattta taaataataa aattggctat tctgcaaata ataaattatt
      481 caatagaaat ttatttgata atataagatt cccactgggt aaattaatgg aagataaggc
      541 tactacttat ttgttgatcg atcattccga aaaagttgct gtcaattcat ctgctaaata
      601 tcactattac caaagcccta aaagtatact gagaggaagc ttcaatccta gaatttttga
      661 ttcttttgaa gtccatgaag atatactagt attcatagat aagttttatc cggaattatc
      721 aattaatgtt agaactcgct atgtctatga agcaataagg atgatgatga caatgattaa
      781 aaataactat accaataaag aagattattt acgttctata aatattttac aacgctttga
      841 aaaagaagtg aaaattgatc ccgacttttc accaaaatta aaaaaattag taaagttttt
      901 attaataaat aaaa
//
LOCUS       DAGCYL010000314          900 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.329, whole genome shotgun sequence.
ACCESSION   DAGCYL010000314 DAGCYL010000000
VERSION     DAGCYL010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.329"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..887)
                     /locus_tag="MB216_002816"
     CDS             complement(<1..887)
                     /locus_tag="MB216_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4527568.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPS"
BASE COUNT          302 a          107 c          161 g          330 t
ORIGIN      
        1 tttgaagggg ataaccctgt atatttttta aaagctgaag aaaaatgatg gatcgaatga
       61 aatcctaata tatctgcaat ttctgtatag gttaattcat tttctagcac gagacgtttt
      121 gctttttcca tttttagttc tttaaaatag ctaataacac tttttccatt cacttttcta
      181 aaatgttttt ttaagctggc tggactgata aaaaaaattt gggctaattg ttcaagcaaa
      241 aaattttctg aaagatgttc ttctaaatat cgtatgattg gatcaactaa ttcatctgaa
      301 taaagaatat gctttttttt aggaatgact ttctcatttg aaggtattgt caataaagca
      361 agttcctgtt gataatgcct tggtaaggaa gacaattgaa ttttttgcag acaataagta
      421 actaataagg aaagcaaatg aatgctcatc tgttggcata gtatgaaaaa agaattgatt
      481 tcctgatcat aggaaatagc tggcttacta tgttttagat aatcaatttt gaactgttca
      541 tttttcagaa aagtaatgat agatgttatt tcttgatcta tagaaaaaga ttgtcccgta
      601 atatgtgcta ataattgctc atcaaaataa aactggataa tgataaaagt ttgtgtttcc
      661 ttttgagtag aaaagatttg gacttcttgc tgtttatcaa tgaataaaag ggaaggatta
      721 tctagttcta tagatttatg tgcaaattca gcatataaat tagcgctttc atttattagt
      781 agtcgaaaat agggagcagc agataaatga agatgggtat ctatggaata ggttagtgtt
      841 tttttgacaa gattggagga ttggttgttt gttaacaaaa gaaccacttt cctttctctt
//
LOCUS       DAGCYL010000315          885 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.330, whole genome shotgun sequence.
ACCESSION   DAGCYL010000315 DAGCYL010000000
VERSION     DAGCYL010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.330"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>885
                     /gene="bsh"
                     /locus_tag="MB216_002817"
     CDS             <1..>885
                     /gene="bsh"
                     /locus_tag="MB216_002817"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBT4527569.1"
                     /translation="DHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVNDLDTHYAMIGI
                     AAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTV
                     GETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTN
                     NPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKAT
                     FTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYY
                     RTYEDSQITAIDMNKEDL"
BASE COUNT          305 a          117 c          162 g          301 t
ORIGIN      
        1 tgatcattat tttggaagga attttgatta tgaaatatct tacaatgaag tagttactgt
       61 tactccaaga aattataagt tgaattttcg aaaggtaaat gatttggata ctcattatgc
      121 aatgattggt attgccgctg gtatagctga ctaccctctt tattacgatg cgacaaatga
      181 aaaaggattg agtatggctg ggctaaattt ttctgggtat gctgattata aagaaataca
      241 agaagggaaa gacaatgtat ctccttttga atttattcct tggattttag gacaatgctc
      301 aacagtagga gaaactaaaa aattgttaaa aaatatcaat ttagcaaata taaattatag
      361 tgatgaactt cctttatccc ctttacattg gctattagct gataaagaaa aatcaattgt
      421 cattgaaagt atgaaagatg gacttcatat atatgataac cctgtgggcg ttcttaccaa
      481 taatccttca tttgactatc aattatttaa tttaaacaat tatcgtgtct tatcgagtga
      541 aactcctaaa aataattttt caaatcaaat aagtttgaat gcctatagcc gcggtatggg
      601 agggataggc ttgcctggag atttatcctc agtatctcgt tttgttaaag cgacttttac
      661 gaagctgaat tctgtatctg gagattcaga gtcagaaagt attagtcaat ttttccatat
      721 cttaggttca gtagaacaac aaaaaggttt gtgtgatgtt ggtgatggaa aatatgaata
      781 tacaatttat tcttcttgtt gcaatgttga caaaggaatc tattattatc gaacatatga
      841 agacagtcaa attactgcaa ttgatatgaa taaagaagac ttaga
//
LOCUS       DAGCYL010000316          872 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.331, whole genome shotgun sequence.
ACCESSION   DAGCYL010000316 DAGCYL010000000
VERSION     DAGCYL010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.331"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            108..>872
                     /locus_tag="MB216_002818"
     CDS             108..>872
                     /locus_tag="MB216_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBT4527570.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNH"
BASE COUNT          375 a           97 c          142 g          258 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc at
//
LOCUS       DAGCYL010000317          851 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.333, whole genome shotgun sequence.
ACCESSION   DAGCYL010000317 DAGCYL010000000
VERSION     DAGCYL010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.333"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>851)
                     /locus_tag="MB216_002819"
     rRNA            complement(<1..>851)
                     /locus_tag="MB216_002819"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          271 c          188 g          217 t
ORIGIN      
        1 catctcacga cacgagctga cgacaaccat gcaccacctg tcactttgcc cccgaagggg
       61 aagctctatc tctagagtgg tcaaaggatg tcaagacctg gtaaggttct tcgcgttgct
      121 tcgaattaaa ccacatgctc caccgcttgt gcgggccccc gtcaattcct ttgagtttca
      181 accttgcggt cgtactcccc aggcggagtg cttaatgcgt tagctgcagc actgaagggc
      241 ggaaaccctc caacacttag cactcatcgt ttacggcgtg gactaccagg gtatctaatc
      301 ctgtttgctc cccacgcttt cgagcctcag cgtcagttac agaccagaga gccgccttcg
      361 ccactggtgt tcctccatat atctacgcat ttcaccgcta cacatggaat tccactctcc
      421 tcttctgcac tcaagtctcc cagtttccaa tgaccctccc cggttgagcc gggggctttc
      481 acatcagact taagaaaccg cctgcgctcg ctttacgccc aataaatccg gacaacgctt
      541 gccacctacg tattaccgcg gctgctggca cgtagttagc cgtggctttc tggttagata
      601 ccgtcaaggg atgaacagtt actctcatcc ttgttcttct ctaacaacag agttttacga
      661 tccgaaaacc ttcttcactc acgcggcgtt gctcggtcag actttcgtcc attgccgaag
      721 attccctact gctgcctccc gtaggagttt gggccgtgtc tcagtcccaa tgtggccgat
      781 caccctctca ggtcggctat gcatcgtggc cttggtgagc cgttacctca ccaactagct
      841 aatgcaccgc g
//
LOCUS       DAGCYL010000318          850 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.334, whole genome shotgun sequence.
ACCESSION   DAGCYL010000318 DAGCYL010000000
VERSION     DAGCYL010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.334"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(399..773)
                     /locus_tag="MB216_002820"
     CDS             complement(399..773)
                     /locus_tag="MB216_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBT4527571.1"
                     /translation="MENIFDLNKGLSRRKYWYYQCILFVFGLGIETFSMFVYKKSLIE
                     NNYLKYFVINGPFDFYNFLRIVFFIMIIIGFLFQARRFVDIGWSKFVALLSLLPLVGI
                     ITTGICLFKKGKDNKTDSSQVS"
BASE COUNT          323 a          158 c           92 g          277 t
ORIGIN      
        1 taatatatct atcaacaatc tactttaaaa acttatttca ttgaatagct agtgaaatat
       61 aactagcttg tttcatactt acaaaatcat tcttgtacga aaaagtaccc taccttttaa
      121 atttatcatg tattatccgt tagtaaactt tactactgca tatgcccaaa gtgacgatgg
      181 ttattaggac aactttgcaa aaagtaaagt ccctaaacat ttatggaata aatatcaaca
      241 agctaaagca aaacaaggtg cttgtattat gaaaggaatt tggtctgcag tagccttcca
      301 taaactctca tggtcagctg cgattcaagc tgcttacaat gcaagttctt gtcattcatt
      361 taatagacaa tactcttaat aaataaaaca gatagcaatc aagaaacttg actgctatct
      421 gttttattgt cttttccctt tttaaataaa caaatccctg ttgtgataat accaactaac
      481 ggtaacagac ttagtaaagc tacaaatttc gaccacccaa tatcaacaaa ccttcttgct
      541 tggaacagaa aacctataat aatcattata aagaacacga tcctaagaaa attataaaaa
      601 tcaaatggac catttatcac gaaatatttt aaataattgt tttcaatcag agactttttg
      661 tatacaaaca ttgaaaaagt ttctatccct aatccaaaca caaacagtat acactgataa
      721 taccaatatt ttcttcttga taatcccttg tttaaatcaa aaatattttc caaatctata
      781 tcccccaaaa cctactaatg ctactttatg tatttattta caataccatt gtaattggca
      841 aatacataaa
//
LOCUS       DAGCYL010000319          849 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.335, whole genome shotgun sequence.
ACCESSION   DAGCYL010000319 DAGCYL010000000
VERSION     DAGCYL010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.335"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..277
                     /locus_tag="MB216_002821"
     CDS             <1..277
                     /locus_tag="MB216_002821"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBT4527572.1"
                     /translation="YEKVQIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDK
                     IILMAYCELNENEVKDHRPKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            479..849
                     /locus_tag="MB216_002822"
                     /pseudo
     CDS             479..849
                     /locus_tag="MB216_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          340 a          117 c          162 g          230 t
ORIGIN      
        1 atatgaaaaa gtacaaatag ttgatgttaa taatggaaat agatttgaaa cttatacaat
       61 agctggagag agaggaacag ggatgatttg cttgaatgga gctgcagcta gatgtgtatc
      121 aactggagat aaaataattt taatggcata ttgtgaatta aatgaaaatg aggttaagga
      181 tcatagacct aaggttgttt ttgtcgatga taataataaa gttgaaagag taacatcata
      241 tgaaaaacat ggaaggttat cagatataat tctttaggtt ttgtaatgtt atttatatat
      301 agctagacta ttattgaaat atttattgat tactaattgg atagatgtca ataaagtaga
      361 cacaaaaaga gaagatacag actgtagaca aagtccattt tatgtgaatc ggctacacta
      421 gactagacag aaaaaataag gtgtgtagaa taataaaaaa cacactggag gatttttcat
      481 gtcaagacgt caacgaagaa cctattcaaa agaattcaaa caacaaatcg tcaatctcta
      541 tctcgctggt aagcctcgcg cagaaattat tcgagagtat gagcttacgc cttcttcttt
      601 cgataaatgg atgaagcaag cacaatcaac gggctcattc aaaaaaagag acaacttaac
      661 accagaacaa gcagaattga tcgcactaag aaagaaaaat aagcaactcg aaatggagaa
      721 tgatatttta aagcaagcgg cgctgatatt cggacgaaaa gacaagtaat tgatgccaac
      781 aagcataaat attccatatc agcgatgtgc aaaattctaa atatttctcg tcagacctac
      841 tattatcaa
//
LOCUS       DAGCYL010000320          827 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.336, whole genome shotgun sequence.
ACCESSION   DAGCYL010000320 DAGCYL010000000
VERSION     DAGCYL010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.336"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            68..727
                     /locus_tag="MB216_002823"
     CDS             68..727
                     /locus_tag="MB216_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfX family protein"
                     /protein_id="HBT4527573.1"
                     /translation="MEENNIESKDNLFKEIGEKMYPLFFNRNFGLATKSEIESMLFYS
                     YLKHKINSNSDYSDRTLSRELGISEVKVRNLKRTCYARYEDAIDFPKLLTSLSKEGSN
                     LMFFKIYTDNNDVLCRISISEVVYYDELRTQLNNSGILFKKNPGSNYVELSMADAIIF
                     FDQDSISKESLDLKNLIDKMKQLNFKTNTKMDIDKILEGTKAGRMKNVVEAMMKLSNS
                     I"
BASE COUNT          320 a           96 c          145 g          266 t
ORIGIN      
        1 gatgttaaga atggttttcc ctagtaatag gtaacatcaa aataaaggaa ggtgtaattc
       61 tatacctatg gaagaaaata atatagaaag caaagacaat ctgtttaaag agattgggga
      121 aaaaatgtat ccgctttttt ttaatcgtaa ttttggatta gcaacaaaaa gtgaaattga
      181 aagcatgctc ttttactcgt atctaaaaca taagataaat agtaatagtg attactcaga
      241 tagaaccttg tctagagagc taggaatatc ggaagttaaa gtaaggaatc ttaagagaac
      301 atgttatgca agatatgaag atgcaattga ttttcctaaa ttactaacct cgctgtccaa
      361 agaaggctca aatctaatgt tttttaaaat ctatactgac aataatgatg tattatgtag
      421 gatatctatt tccgaggtag tttattatga tgaacttcga acacaattaa acaatagtgg
      481 tattcttttt aaaaaaaatc caggatcaaa ttatgtagag ttatcaatgg cagatgcaat
      541 aattttcttt gatcaggatt ctatcagcaa agaaagctta gatttgaaaa atttaataga
      601 taagatgaaa cagttgaatt ttaaaactaa tactaaaatg gatattgata aaatattaga
      661 aggaactaaa gctggtagaa tgaaaaatgt cgttgaggct atgatgaagc tttccaactc
      721 aatataaaca aagaggtcag aaacttccta agttttttga cctattgctt tttcgtgtgt
      781 aactttttta tttaaatggc ataagttttt atcgatgaat ggcaaaa
//
LOCUS       DAGCYL010000321          824 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.337, whole genome shotgun sequence.
ACCESSION   DAGCYL010000321 DAGCYL010000000
VERSION     DAGCYL010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.337"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..748
                     /locus_tag="MB216_002824"
     CDS             <1..748
                     /locus_tag="MB216_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HBT4527574.1"
                     /translation="LYSIYKDQDSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIW
                     NNLQWEKEIEKPQIGSVFSARLKLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTK
                     LNKYVTFSVNNEDFWSSLDVNYSKGKTERGRVFYISISYNTADNQPIYSVYTDETCTE
                     WRGYYKGNNFEDTQITMLENKSVKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFY
                     NFTNNAYYYELKKDGRVYGYINSEAAVEMN"
BASE COUNT          305 a          106 c          154 g          259 t
ORIGIN      
        1 tctatattct atttataaag atcaggattc ctctgaatgg ttagggtatt taaaagcaca
       61 tgttagttta gcctatactc ctattgaaga aattaataaa aacgtaactg ttactaaaaa
      121 ttggtcgatt tggaataatc tacaatggga aaaggaaatt gaaaagcctc agattggttc
      181 tgtattttca gctagattaa agttaaccaa tgtatcgaat aatgctgtct attacaagct
      241 ttataaatct ggaaaatttt atggttatat caatgctgag gctgtcaaag accttacaac
      301 aacaaagctt aataagtatg ttacatttag tgttaataat gaagattttt ggtcttcttt
      361 agatgtaaat tattcaaaag gaaaaactga aagaggccga gtattttaca ttagtatttc
      421 atataatacc gcagataacc aaccgattta ttcagtatat acagatgaaa catgtacgga
      481 atggcgaggg tattataagg gaaacaactt tgaagacact caaattacta tgcttgagaa
      541 taaaagcgtg aaagtaacaa aaagtgggta tacagtatgg ggagacttga atttttggac
      601 taagtcagga atatcgaaca caggagatat atacaccact gaccgaaaat tttataattt
      661 taccaacaat gcttactatt atgaattgaa aaaagatggg agggtctatg gctatataaa
      721 tagtgaagca gctgtagaga tgaactaaaa tattgctgtc ccttaatctt atggggcagc
      781 aatattttta tattattaat catttttctg aagcgagggt agaa
//
LOCUS       DAGCYL010000322          781 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.338, whole genome shotgun sequence.
ACCESSION   DAGCYL010000322 DAGCYL010000000
VERSION     DAGCYL010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.338"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..719)
                     /locus_tag="MB216_002825"
     CDS             complement(<1..719)
                     /locus_tag="MB216_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBT4527575.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIGDN"
BASE COUNT          231 a          138 c          102 g          310 t
ORIGIN      
        1 taattatctc caataatagc tttcttaatt tcatgactaa attgtaaatc attccatatt
       61 gaccaagctt ttgtaacagt taccatttta ttatagtcaa tataagggta gtcaggttca
      121 tcaggcaaaa ttctttttgc aaagttagga taaaaatatt gtatgttaac ctcttttaca
      181 gtttctccag gttgcggtgc atgaatcatt acaccgttac ctttatatat acctacatga
      241 taagttgcac ctcgattgcc gtaaaataat aaatcaccag gcttaagtga atttaaactt
      301 acctctgtac cgtattgctc ttgattggtg gttcccatag gcagttcaat atttacagct
      361 tgtttgtaaa catatttcac taaaccacca cagtcaaaag aatttggtcc cgatgctccc
      421 cattcgtatg gctttccaat ttgctccatt gctttattaa tgactttggt ttgattagaa
      481 gtcatagtat aatttactat tcttgtttca ctattttcat ttgataaatt tgtaggtatt
      541 tccataatct gcttaaactg ttcttcacta taaccaactt ttaaagcttt ttctttttct
      601 atattatact gttcttctgt gtagtttatt tgttctgaaa aaacaatagt attgtagtac
      661 acactcaaaa cgattgagaa agacatcaca agaaataaag ttaatagttt tttcttcatt
      721 ttttacctct tctccttatt ttttacaagc gcttgcattt taatattatc attgtttaag
      781 a
//
LOCUS       DAGCYL010000323          767 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.339, whole genome shotgun sequence.
ACCESSION   DAGCYL010000323 DAGCYL010000000
VERSION     DAGCYL010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.339"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            32..733
                     /locus_tag="MB216_002826"
     CDS             32..733
                     /locus_tag="MB216_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBT4527576.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          271 a          143 c          136 g          217 t
ORIGIN      
        1 atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt
       61 ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat
      121 caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga
      181 tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga
      241 aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg
      301 aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt
      361 ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta
      421 tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt
      481 gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg
      541 cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc
      601 agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc
      661 cgggctgtta gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc
      721 tatgagagaa taacgcgaac aaaaataagc agtgaccctg ataaata
//
LOCUS       DAGCYL010000324          761 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.340, whole genome shotgun sequence.
ACCESSION   DAGCYL010000324 DAGCYL010000000
VERSION     DAGCYL010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.340"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            51..731
                     /locus_tag="MB216_002827"
     CDS             51..731
                     /locus_tag="MB216_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HBT4527577.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
BASE COUNT          270 a          127 c          152 g          212 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat aagaaaaccc
      661 gaaaagaagg cactctcttc gggttttcgg tctgtactga aatcaaggta ttattgggaa
      721 tcccagctta aatcatagat accgtaaggg attttattct t
//
LOCUS       DAGCYL010000325          751 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.341, whole genome shotgun sequence.
ACCESSION   DAGCYL010000325 DAGCYL010000000
VERSION     DAGCYL010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.341"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>751
                     /locus_tag="MB216_002828"
     CDS             <1..>751
                     /locus_tag="MB216_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBT4527578.1"
                     /translation="YHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVD
                     EFQDTRELQYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQ
                     SKTLHGCYRSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKN
                     IIKEKIESGIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFF
                     KLAILYFTESGEKVRRRKLIANDILIILKNEF"
BASE COUNT          286 a           97 c          128 g          240 t
ORIGIN      
        1 gtatcacact atgttggaaa aaaataaaga aattgatttt gatatgattt tactagtagc
       61 gtataaaatt ttaactgtaa acagtaacgt tgcacgtaat attgctagta atataagggc
      121 gatttatgtg gatgaatttc aagatactag agaattacaa tacaatgtaa tagctaaatt
      181 acttcaaaac aaccctcaaa ttcagtcaat gtttgttgga gatattgacc aagcaattta
      241 tggatcatta gatggtatag ctaaatcagt tgaagaacta gaaatgctaa ctagtcacac
      301 ttttcaatca aagacgctac atggctgtta caggtctaat cagagattag tagacttcta
      361 ctctaatttt cagagttgtc cttacgagat tgaaagcaga gggaataata aaaacgaccg
      421 tggttttata agttatgatt gcaaagttac taaggaggat ttacccaaca taataaaaaa
      481 tataataaag gaaaagattg aaagcggcat tcatgaaaaa gatatttgtg tacttgctcc
      541 tcaacactat cctctgtttt cactgggtga gtatttaaag aaagaattac cgtattgcaa
      601 ttttgatgct ataaatatat caccaattaa ggtaaataat catagtttat ttttcaagtt
      661 agcaattttg tactttactg aatcaggtga aaaggttcga agaagaaagc taattgcaaa
      721 cgatatttta attatattaa aaaatgaatt t
//
LOCUS       DAGCYL010000326          741 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.342, whole genome shotgun sequence.
ACCESSION   DAGCYL010000326 DAGCYL010000000
VERSION     DAGCYL010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.342"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            200..>741
                     /locus_tag="MB216_002829"
     CDS             200..>741
                     /locus_tag="MB216_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527579.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
BASE COUNT          311 a           82 c          135 g          213 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat a
//
LOCUS       DAGCYL010000327          740 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.343, whole genome shotgun sequence.
ACCESSION   DAGCYL010000327 DAGCYL010000000
VERSION     DAGCYL010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.343"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(415..639)
                     /locus_tag="MB216_002830"
     CDS             complement(415..639)
                     /locus_tag="MB216_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527580.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          274 a          104 c          134 g          228 t
ORIGIN      
        1 tttcaaagaa ggagtatgaa aatgagaaaa atatttattc gtttattgtg ggtactatct
       61 tagaggaata aataatagtg gagaaaacta tggtttggcg cagattctat caggtttgat
      121 ggaacgaaga caacctcccg acatcaagtc aaaagctact tgagattttg atctgaaaat
      181 aaaaaaagaa aacaaggtag gaacatcaat gtttctacct tgttttcgta atcaatggga
      241 aaaaactaag agtggtgtac gtaatcaata aaaaggatgg tgacaacagt acctctttac
      301 tctctgaaag catcataact cgtttttcct cattatgcca ataaattgtt gttataccac
      361 atataaggat ttgtaatagc taaaaacaga caggaaagtt gaaatctgtg aatcttattt
      421 tgtatggaag gatttataga acgcaaaaat caagataaag atgaataaaa atcctgtttc
      481 ggctatatat tgcgtatcta cgccaataaa acgagtttgt tctgtcatag ccatataaag
      541 caaaggcaaa aatacaacta cagcgagata agatccgatg atttctcgaa aagttttcca
      601 ggaagtatgt ttcctgtttt tatatttatc cgatttcatt gaatagtcat agcctcctta
      661 ttctttcata tgttgaaatt ccagtataaa aactggaagg aactgtacgt tttttagaat
      721 aaaaattata agggataagt
//
LOCUS       DAGCYL010000328          720 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.344, whole genome shotgun sequence.
ACCESSION   DAGCYL010000328 DAGCYL010000000
VERSION     DAGCYL010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.344"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>720
                     /locus_tag="MB216_002831"
     CDS             <1..>720
                     /locus_tag="MB216_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4527581.1"
                     /translation="SFEPTDSFQYLSKDIEIEDIYKILAQELSKNVISLPKVFYFPAE
                     RTGINVFKNELNESRLKTYDTLLNTVQFTNLTNQKKKAALKYDLLKKNIELLMETTDN
                     SSYPKPISDYINYLNNMKNRYEQKNVNNIASYIRDEIIMGKYEIDEKDNSVSFRQKFG
                     KNRYRKERIPFHVVSSSIKSLYGLDYYLENSSKEGDYLIIDEPELSLHPENQVKLAKV
                     ITDIVESKIKLITSTHSDLFVR"
BASE COUNT          285 a           88 c          116 g          231 t
ORIGIN      
        1 tagctttgag ccaactgatt catttcagta tcttagcaaa gatattgaaa ttgaagatat
       61 ttataaaatt ttagctcaag agctatctaa aaatgttatt agtttgccaa aggtttttta
      121 ttttccagcc gaaagaactg gtataaatgt ctttaagaac gaactaaatg aaagtcgctt
      181 aaaaacatat gataccttgc tgaatactgt gcaatttact aatttaacta accaaaaaaa
      241 gaaagctgcc ctcaagtacg atctgttaaa aaagaatatt gagctgttaa tggagacaac
      301 ggataatagt tcttatccaa agccaatatc ggattatatt aattatctga ataacatgaa
      361 aaatagatat gaacagaaaa atgtcaataa tatagcatca tatattagag atgagattat
      421 tatgggtaaa tatgaaattg acgaaaaaga taattctgta agttttagac aaaagtttgg
      481 taaaaataga tatagaaaag aacgaattcc ttttcacgtt gtgtcttctt cgattaaatc
      541 tctatatgga ttagattatt acttagaaaa ttcaagtaaa gaaggagact atcttattat
      601 tgatgagcca gaattgagtt tacatccaga aaatcaggtt aagttagcaa aagtaattac
      661 agatatagtt gagagtaaaa ttaagctaat tacttctacc catagtgatt tatttgtacg
//
LOCUS       DAGCYL010000329          717 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.345, whole genome shotgun sequence.
ACCESSION   DAGCYL010000329 DAGCYL010000000
VERSION     DAGCYL010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.345"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>717)
                     /locus_tag="MB216_002832"
     CDS             complement(<1..>717)
                     /locus_tag="MB216_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBT4527582.1"
                     /translation="MFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNLNVAMADRERL
                     YNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKTIEQIKIDNLL
                     SQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSII
                     VFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTK
                     IEHLSPLISSVVQFIGLNIY"
BASE COUNT          247 a          117 c           88 g          265 t
ORIGIN      
        1 ttatagatat ttaagccgat gaattgaacg acagaagaaa ttaagggaga aagatgctct
       61 atcttagttt ttccgatttc cttagtataa taaacaatcc cagaatccct cacttttaag
      121 acctcaggta acgatacagc taattcgttt tcttgtatca aagcatctct actttgatac
      181 gcttctatta tatccattcc taatgttata gacgtttgtg ctaacttttc aaaaacaatg
      241 atagaataat ttttttcaga ccgaatcgta tctccactaa cacttggaat tattccatta
      301 ctcagattaa aaaccatgtt ttctaaattt cgaatatctc catttttaac atattccagt
      361 attttatttt cataaagaaa cagatatatt tctggatcgt aattttgtga taacagatta
      421 tctattttga tttgctcgat agttttactt agatttcctg tatattcaat taacggttca
      481 tggaatagat cttctatttt tccagtaaaa aaataatgaa ctaaaatcag aatatctcga
      541 atgtctccta aagaatacaa agtcaaattt tgcaatgaat tatagagtcg ttctctatca
      601 gccattgcaa cattcaaatt gttcattttt agtttaaaaa aatctttatc aatattattt
      661 gctctaaatg gaccaaataa gtaatatctg gttttaaaca tgtatagaag aaacatc
//
LOCUS       DAGCYL010000330          712 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.346, whole genome shotgun sequence.
ACCESSION   DAGCYL010000330 DAGCYL010000000
VERSION     DAGCYL010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.346"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            63..251
                     /locus_tag="MB216_002833"
     CDS             63..251
                     /locus_tag="MB216_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBT4527583.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(309..662)
                     /locus_tag="MB216_002834"
     CDS             complement(309..662)
                     /locus_tag="MB216_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4527584.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
BASE COUNT          229 a          124 c          108 g          251 t
ORIGIN      
        1 ttaaataaga tgaaatattc tttaatatag atttgaatta aagtggaaag gaggagattg
       61 ttattataaa ctacaagtgg atattgtgtc ctatttgtgg aaataaaaca agactacgaa
      121 tacgagtgga tactatactt aaaaatttcc ctttatactg ccccaaatgt aagaacgaaa
      181 ctttaattaa tgttcaaaaa atgaatataa taacaatcaa agagccagac gccaagacgc
      241 agagccgata atttgagaaa tgaaactctc atcttatcgg ctctttttgt ttatctgaat
      301 tttactgact agccttcaat atttcttgct tcattgatac cttttgctag tgcttccatt
      361 aaggatagtt ctttgtctgt aaagctatcc atgtattttt ctacctgtaa ccttcgagtg
      421 cttttaatca aattatctgt aggtaagaaa aattcatcaa tagatacatg gagtaatgat
      481 acaagatcat acagcacttg tgtacttggg tgttgtcctt tgttttctat atttgttaaa
      541 taacgtgggt caatttcaat cattgttccc acttgttcac gagtgagacc ccgtttcatt
      601 cgagcctctt taatcgctaa accaacggct ctaaaatcgt atttatcttc ttttttacgc
      661 atactaaatc acctctatac attttactgt tcctatataa ccataaacag gt
//
LOCUS       DAGCYL010000331          705 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.347, whole genome shotgun sequence.
ACCESSION   DAGCYL010000331 DAGCYL010000000
VERSION     DAGCYL010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.347"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..79
                     /locus_tag="MB216_002835"
     CDS             <1..79
                     /locus_tag="MB216_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4527585.1"
                     /translation="YEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            80..>705
                     /gene="aac(6')-Ie"
                     /locus_tag="MB216_002836"
     CDS             80..>705
                     /gene="aac(6')-Ie"
                     /locus_tag="MB216_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HBT4527586.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIG"
BASE COUNT          299 a           51 c          129 g          226 t
ORIGIN      
        1 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
       61 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      121 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      181 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      241 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      301 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      361 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      421 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      481 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      541 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      601 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
      661 gcattacttt gataatttca aagtagatag tattgaaata atcgg
//
LOCUS       DAGCYL010000332          649 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.348, whole genome shotgun sequence.
ACCESSION   DAGCYL010000332 DAGCYL010000000
VERSION     DAGCYL010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.348"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            420..>649
                     /locus_tag="MB216_002837"
     rRNA            420..>649
                     /locus_tag="MB216_002837"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          236 a          107 c          136 g          170 t
ORIGIN      
        1 gattccttta aggtgtcact aaaaattata aaaaataggg ttgacgcact tttgatgtca
       61 tgttataata acgaagtcgt ttggatgagg tagctaataa agcttgattt tatcgaaaaa
      121 aacttcgaaa aagttgttga caaataacaa taaagatgat atgataatca agtcgctgct
      181 aatgagtgac aaagacgttt agaaaaaata aacaaagaag ttgttgacat tctcttctga
      241 atctgataag attaaagagt tgttgaaaca aaaagattag acctttgaaa actgaacaaa
      301 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa acatttttaa
      361 caagcaagca atatgctagc aaacaaattg agcttaacaa tcgcaagatt gttcgaactt
      421 tttatgagag tttgatcctg gctcaggacg aacactggcg gcgtgcctaa tacatgcaag
      481 tcgaacgctt ctttttccac cggagcttgc tccaccggaa aaagaggagt ggcgaacggg
      541 tgagtaacac gtgggtaacc tgcccatcag aaggggataa cacttggaaa caggtgctaa
      601 taccgtataa caatcaaaac cgcatggttt tgatttgaaa ggcgctttc
//
LOCUS       DAGCYL010000333          634 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.349, whole genome shotgun sequence.
ACCESSION   DAGCYL010000333 DAGCYL010000000
VERSION     DAGCYL010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.349"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            18..560
                     /locus_tag="MB216_002838"
     CDS             18..560
                     /locus_tag="MB216_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBT4527587.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
BASE COUNT          199 a          101 c          131 g          203 t
ORIGIN      
        1 tgaaaggagt tttacaaatg attgatccaa aaatgttggg gatgatcttc ctcattaatt
       61 ttgcttatat cactctgaac acgttacgtt tcatgttgac aatgaaaggc tatcgactga
      121 ttgctccact tgtcagtatg gtcgagatca cgatttatat cgtcggctta tcccttgttt
      181 tagatcgttt agataatcca ctgaatttat ttgtctatgc gttaggttac gcagtaggta
      241 tcagtgtcgg aattttgatt gaagacaaat tggcattagg atacatcatg gtcacaacga
      301 ttttgccagc aaatagcgca gaagataaaa gtttgccggg tatactgaga gaacacggtt
      361 atggtgtgac acagacttct gcaatggggc tggaaggtga gcggctagtt ctcgaaatac
      421 tttctcctag aaaaagcgaa cgtgaactgt atcgcctgat caaagaaatc gaagagcgtg
      481 cttttatcat ttcttatgag ccaaaatata tttcaggtgg tttctggact aaaaaagtga
      541 gaaaacgaca aggaaaataa ctcacaaact taaaaactgt ctaatatcta aattaaattt
      601 taaatgttag acagttttta tttattattt tgat
//
LOCUS       DAGCYL010000334          627 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.350, whole genome shotgun sequence.
ACCESSION   DAGCYL010000334 DAGCYL010000000
VERSION     DAGCYL010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.350"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            330..>627
                     /locus_tag="MB216_002839"
     CDS             330..>627
                     /locus_tag="MB216_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4527588.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFP"
BASE COUNT          230 a           69 c          104 g          224 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaa
//
LOCUS       DAGCYL010000335          613 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.351, whole genome shotgun sequence.
ACCESSION   DAGCYL010000335 DAGCYL010000000
VERSION     DAGCYL010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.351"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
BASE COUNT          212 a           92 c          102 g          207 t
ORIGIN      
        1 cgaacgagat gatcttgaga aactagatca tcaatcgtaa gcatcgcaaa ttgatttcta
       61 ccattcgtat tgtttttaga catcatagct atcactcctt gcattcgttt tcataaatct
      121 attataaaag aaaagactgt agacatcatc cataaaatgg actttgtcta cagtctggat
      181 aacgtgattt tttgctgtaa tgacaaggtt ttagctgttt cagataccat gtatccaaaa
      241 atagtttatt gagaaaatag gagagtattt gatactaact agtaggaaaa aatgagataa
      301 aaagtaaatg gaatcatttc aggtaatctc aaataaaaaa atatacagtc ctgtggcact
      361 ttacctatgg gactgtatat attttttact ctcagcgggc ttgttttgac tccgtgtctg
      421 actccgtcgt attgatttta aatgatattc gatgaccatt taacaggtca tttttgaaat
      481 ttttgtctct atatgactca gtttgataat tattgataga tcaatttggg tctatatcga
      541 acaagggaat aatccaataa aaaatgattt agaatatcaa aataacaaaa aagaggacta
      601 cctgtattag tcc
//
LOCUS       DAGCYL010000336          579 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.352, whole genome shotgun sequence.
ACCESSION   DAGCYL010000336 DAGCYL010000000
VERSION     DAGCYL010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.352"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
BASE COUNT          198 a          101 c           74 g          206 t
ORIGIN      
        1 gtagtcatta agtcttcata agatgtacaa tatgacatta aaaaaataaa agcttacgac
       61 catccgttaa atctgacact aaataatgga ctgctttctt ttgtattagc aggttacgtt
      121 gactatgaat attcaccaga ttatttggag aaacctcttc aacacgagaa caatgatatt
      181 aattttagat ttgtctacga gtcaaaaagt aaaacatggt tagttgatca attaagcacg
      241 ataaacacaa gcttccatga attacctaaa cctgaaaatg aagttgagat ggtcattaaa
      301 atcttctaaa aaagaagcgt tgaaggggaa atattccttt caacgctctt ttttatatgc
      361 ttagaaacat aaataaattg tatactttct ttttagcccc tattactttt cacgaaaaat
      421 atcttttttt cgacgctagc gtttctctac ttctaatact gtacacagtt tttgtattat
      481 tgaaaaatta ctcattattt agcattaatc cacatcgttt tatcaatcga ccttcatact
      541 aaactacttt aaagtcatct tccttctcgt ttttcatta
//
LOCUS       DAGCYL010000337          575 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.353, whole genome shotgun sequence.
ACCESSION   DAGCYL010000337 DAGCYL010000000
VERSION     DAGCYL010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.353"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            22..492
                     /locus_tag="MB216_002840"
     CDS             22..492
                     /locus_tag="MB216_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBT4527589.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          204 a          109 c           91 g          171 t
ORIGIN      
        1 aaatagaaag gagaaaactt tatgtctatt tataattttg aaactacttt agaagacggc
       61 actgtctatt cattgaaaaa atatgcaggc aaaccgataa tcattgttaa cactgcaaca
      121 aaatgcggtc tagcacctca atttaaacaa ttagaagaac tttaccaagc ttatcagaca
      181 caaggtctgg ttatcttagg ctttccttcc aatcaattta aacaagagct agattcagcg
      241 actgaagcag cagaagcttg taaaaccact tatggcgtta ccttccctat gcatcaattg
      301 attgacgtaa atggagaaaa tacagatcct ctttttactt atcttaaaaa tgaagctcct
      361 ggtactgtca ctaaagcaat aaaatggaat tttactaaat ttttgattga tagaaatggc
      421 aaagtaatcg aacgatttgc accacaaact tctccattga aaatgaccgc tgcaattgaa
      481 tccgttcttt aacttaataa aaataaaaaa agatgaacta tttctagttc atctttttgc
      541 ctttgcgtgg cgacgtccta ctctcacaag gggca
//
LOCUS       DAGCYL010000338          562 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.354, whole genome shotgun sequence.
ACCESSION   DAGCYL010000338 DAGCYL010000000
VERSION     DAGCYL010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.354"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            299..>562
                     /locus_tag="MB216_002841"
     CDS             299..>562
                     /locus_tag="MB216_002841"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBT4527590.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLS"
BASE COUNT          193 a          101 c           82 g          186 t
ORIGIN      
        1 attattcttt tcataaatat tgtaaataga agtattaaaa atatatacaa aaagtattat
       61 ttcgagaaag ataaatattt tcaaaaacct tttcataatc ctcacccttt cttacactga
      121 taaatcatgc gtctaatatt caaattatac tcgatatgct ttagctaatc agccaactct
      181 gtttgtctac tatcattata tcaatagaca atatctgctc ccgtctttat gatcgtgcca
      241 aagcaatcaa aagaaaattg cgaatcactt agatcgaaag tttactcaaa caaaagcatt
      301 gtcagcggtt gtcacggatt taacttatgt tcgggtcggg acaaattggg cttatatttg
      361 tttgatgatt gatttattca atcgtgaaat tattggccat tcttgcggaa ataaaaaaga
      421 tgcgcagctt gtaaaaaggg ccattcaaag cattccttac tcacttcaag aaatcgaact
      481 cttccatacg gatcgtggaa aagaatttga taaccaaacc attcagaatc tcatgaatgg
      541 tttggttatc aaattccttt cc
//
LOCUS       DAGCYL010000339          530 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.355, whole genome shotgun sequence.
ACCESSION   DAGCYL010000339 DAGCYL010000000
VERSION     DAGCYL010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.355"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>530)
                     /locus_tag="MB216_002842"
     CDS             complement(<1..>530)
                     /locus_tag="MB216_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4527591.1"
                     /translation="IDAAIQFDFIYPIVESTYSTYGRPSIDPVVLIKLVFIQYLFGIR
                     SMRQTIKDVETNVAYRWFLGYSFEDPIPHFSTFGKNYVRRFRETTLFEDIFSHILEQA
                     VKAGFVTEDNLYIDSTHIKANANKHKFTKEMTYGQAKAYQDELEDEINKERIAVGKRP
                     FTWDTESELSLQKISHS"
BASE COUNT          172 a           91 c           89 g          178 t
ORIGIN      
        1 gaatgactta ttttttgaag actcaactca ctttctgtgt cccatgtaaa aggacgtttt
       61 cctacagcaa tacgttcttt atttatttca tcttctaatt catcttgata agcttttgct
      121 tgaccgtagg tcatctcttt cgtgaactta tgtttattgg catttgcttt gatatgagta
      181 gaatcaatat aaaggttgtc ttccgtaacg aagccagctt ttacagcttg ttctagaata
      241 tgagagaaaa tatcttcaaa taaagttgtt tctctaaatc ttcgaacata gtttttacca
      301 aaagtagaga agtgtgggat aggatcctcg aatgaataac ctaaaaacca acggtaagca
      361 acatttgttt caacgtcttt aattgtctgt ctcatcgaac ggataccaaa taaatattgg
      421 ataaatacta atttaattaa gaccactgga tcaatactgg gacgaccata ggttgaatac
      481 gtagattcta caatgggata aatgaaatca aactgaatcg cagcatcaat
//
LOCUS       DAGCYL010000340          530 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.356, whole genome shotgun sequence.
ACCESSION   DAGCYL010000340 DAGCYL010000000
VERSION     DAGCYL010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.356"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            96..494
                     /locus_tag="MB216_002843"
     CDS             96..494
                     /locus_tag="MB216_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4527592.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT          196 a           59 c          103 g          172 t
ORIGIN      
        1 tttttatttt ggtttctata tatgaatcta aatttttgac agcgtttcct aaataagaga
       61 tgatagacga acaaaacaga aaggagacga aacaaatgaa tagtatggtt tttgttaatt
      121 ttcctgttgc tgatgtcaat cgttccgttg ctttttatga aaagctgggt tttaaaaaaa
      181 atgaagaatt ttctactgaa gaagctggtt caatggtatg ggatgacact ttttggataa
      241 tgcttctctc tcatgatttt tataaaaaat ttataaaaga taaagaaata gctgataata
      301 aaaaaacaag tggtgctttg atttcgttta gtgttgaaaa tgcggatgaa gtaaaaaaat
      361 ttgctcaaat tgcaaaagaa aatggtggta ctttccacga agtagatatg ggaatgcctg
      421 aagaagaaat gttttcatta gaagtccaag atctagacgg aaatacatta gaacccgtat
      481 ggatgaagat gtaagcagat aactaatctg acgtttttta taacaaaaaa
//
LOCUS       DAGCYL010000341          529 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.357, whole genome shotgun sequence.
ACCESSION   DAGCYL010000341 DAGCYL010000000
VERSION     DAGCYL010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.357"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>529
                     /locus_tag="MB216_002844"
     CDS             <1..>529
                     /locus_tag="MB216_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4527593.1"
                     /translation="DVETNVAYRWFLGYSFEDPIPHFSTFGKNYVRRFRETTLFEDIF
                     SHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFTKEMTYGQAKAYQDELEDEINKER
                     IAVGKRPFTWDTESELSLQKISHSDPESGYYVKGEREKQFAYSAHTSCEDNGFILSTL
                     ITPGNVHDSQVAIDLV"
BASE COUNT          186 a           90 c           95 g          158 t
ORIGIN      
        1 aagacgttga aacaaatgtt gcttatcgtt ggtttttagg ttattcattc gaggatccta
       61 tcccgcattt ctctactttt ggtaaaaact atgttcgaag attcagagaa acaactttat
      121 ttgaagacat tttctctcat attctagaac aagctgtaaa agctggcttc gttacggaag
      181 acaaccttta tattgattct actcatatta aggcaaatgc caataaacat aagttcacaa
      241 aagaaatgac ctacggtcaa gctaaagctt atcaagatga attagaagat gaaataaata
      301 aagaacgtat tgctgtagga aaacgtcctt ttacatggga cacagaaagt gagttgagtc
      361 ttcaaaaaat aagtcattca gatccagaaa gtggttacta tgttaaaggt gaacgagaaa
      421 aacaatttgc ttattccgcg catacttcct gtgaagataa tggttttatc ttatctacat
      481 taatcacacc aggaaatgta catgacagcc aagtcgcaat cgatctggt
//
LOCUS       DAGCYL010000342          513 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.358, whole genome shotgun sequence.
ACCESSION   DAGCYL010000342 DAGCYL010000000
VERSION     DAGCYL010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.358"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..132)
                     /locus_tag="MB216_002845"
     CDS             complement(<1..132)
                     /locus_tag="MB216_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBT4527594.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          185 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag aca
//
LOCUS       DAGCYL010000343          509 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.359, whole genome shotgun sequence.
ACCESSION   DAGCYL010000343 DAGCYL010000000
VERSION     DAGCYL010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.359"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
BASE COUNT          228 a           70 c           82 g          129 t
ORIGIN      
        1 gaattcctta ggcagctttt gctgtgtaga aaggaataaa taataacgaa agttcaaaag
       61 ctaatgccga ctattctaga cattaaagaa gacgaaaacg aacgataaag aaagaaaagt
      121 tatgaataat cgtttattga aaaaaacttg aaaaaataca aaaaattcgt tgaccaagct
      181 aaatttagtt ggtatattat tacttgtcca aaagacagaa gaaaatattt attcaaaaac
      241 ataacaaaaa agttattgac atgttactaa cgacatgata agataactga gtcgttaaaa
      301 aagaaccact tgcgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
      361 aacaaacgat aagttatgat aaatgagttg ccgaaagatt tcgaaaaacg aaatcgaaaa
      421 aaacttttaa aaaagagttg acaatcgctt gtctctctga tatgatataa aagttgctac
      481 ggcagccaaa aatgaatcgg gaaacactc
//
LOCUS       DAGCYL010000344          503 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.360, whole genome shotgun sequence.
ACCESSION   DAGCYL010000344 DAGCYL010000000
VERSION     DAGCYL010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.360"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            15..>503
                     /locus_tag="MB216_002846"
     CDS             15..>503
                     /locus_tag="MB216_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBT4527595.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT          183 a           91 c           84 g          145 t
ORIGIN      
        1 aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta gtcactaaag
       61 gcgatttaaa tgaattattc cgttcgcact tagaaaaagc gataaacaca ctcctacgga
      121 ctgaattaac ggctttttta gattacgaaa aatatgatcg cactggtttt aattcaggta
      181 attcgagaaa cggttcttac tttcgatcaa tcaaaaccga atatggtgaa ttaacattgg
      241 aaatacctag agatcgtaat ggtgagttta aacaacaaac tttaccagcc tacaaaagaa
      301 caaacgatac attggaaacc actattatcc atttattcga aaaaggtgtt acgatgtctg
      361 aaattgctga tttgatcgaa aaaatgtacg gtcatcacta tactccacaa accatgtcca
      421 acatgactaa agttctgact gaagaagtaa atgcctttaa atccagagcc ttaaatgata
      481 agtatgtcgc tatttttatg gac
//
LOCUS       DAGCYL010000345          496 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.361, whole genome shotgun sequence.
ACCESSION   DAGCYL010000345 DAGCYL010000000
VERSION     DAGCYL010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.361"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(73..270)
                     /locus_tag="MB216_002847"
     CDS             complement(73..270)
                     /locus_tag="MB216_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4527596.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          161 a           90 c           72 g          173 t
ORIGIN      
        1 attacggtga ggaatttttt ctaccgacat aaacaaaaaa accacctcat atgaggcggt
       61 taacgttcaa cattatttta cgatatttac tgcttgagga ccacgttgtc cttcttcaat
      121 atcataagta actgcttggc cttcatctaa agacttaaag ccttctcctt ggattgctga
      181 gaaatgagcg aatacgtcat ttccatcttc accagtgatg aaaccaaaac ctttatctga
      241 gttaaaccat tttactgtac cgttattcat atatatttcc tcctgatacg tatataatac
      301 gtgttttatt gcaattaata ttacttggta aaaggagttt ttatagtgtg aatatatcgc
      361 tcagattacg tttcaaatta gattacttat ttactataac acggatgtaa tgctaacaca
      421 aggataatca ctcgatatac ggtttaaata ttattatttg tcaaattaag cgagacaaaa
      481 ctttccattt acgtaa
//
LOCUS       DAGCYL010000346          485 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.362, whole genome shotgun sequence.
ACCESSION   DAGCYL010000346 DAGCYL010000000
VERSION     DAGCYL010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.362"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>485
                     /locus_tag="MB216_002848"
     CDS             <1..>485
                     /locus_tag="MB216_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBT4527597.1"
                     /translation="GYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     N"
BASE COUNT          172 a           81 c          103 g          129 t
ORIGIN      
        1 ggttatcttt ctgaaatggg cgtaagtaaa gaaaaattag catatgattt agctcatgga
       61 tttaccgatg aaaatccgac aacttcagat gataaaccag tcattgatcc aactagagca
      121 ggtgcagcaa atcctacgct gacagatgga acaaattacg cccacattga tcagtttggg
      181 gaaatcgaaa acgcaaactt gcatgtggct ggatggcaca ttgctaacta taaatacgag
      241 tatatcttca ttatggacta caatactgga aaagaactag ctagagtaag agctgatggg
      301 atttatagac cagacgtaaa tcaagcttat aatactttag gaaatgttgg ctatcatgta
      361 tctttcaata tgcgtaattt tcctaataag aaagtctatg taatgatgcg ggcaacgaat
      421 gatccagaag ggaacactaa aggcggagca caagattttc atgataaacg ctggtacttg
      481 aatat
//
LOCUS       DAGCYL010000347          475 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.363, whole genome shotgun sequence.
ACCESSION   DAGCYL010000347 DAGCYL010000000
VERSION     DAGCYL010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.363"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            127..>475
                     /locus_tag="MB216_002849"
     CDS             127..>475
                     /locus_tag="MB216_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBT4527598.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          190 a           56 c           97 g          132 t
ORIGIN      
        1 tcaagtgatg gaaaatacga ttgctattta agcacaaata gcggagctga aagtaatctt
       61 cttgatgaat taaaaagaac tgagattcaa attatagata aaaaagaacg tcctaaaaat
      121 ggttttgtgc tatcagaaaa aactgattta gaaaatacag aagcatttaa caaagaatcg
      181 gttaaagatt tacgtaaact accaacaaag gacataaatg gcaaggattt tacaagtaaa
      241 gattttgaga agtatgatct tactatggta aatgtatttg caacttggtg tacagcttgt
      301 gtaaaggaaa ttcctgattt ggttgaggtt cagaatgaaa tgaaaagcaa gggtgtaaat
      361 atcgttggag ttgttacaga tcctgttgat gataacggtg aaaacaagga agcaattgaa
      421 aagtcaaagt taatacatga aaagacaaag gcttcatatc ctttcttaat gccag
//
LOCUS       DAGCYL010000348          458 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.364, whole genome shotgun sequence.
ACCESSION   DAGCYL010000348 DAGCYL010000000
VERSION     DAGCYL010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.364"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            353..>458
                     /locus_tag="MB216_002850"
     rRNA            353..>458
                     /locus_tag="MB216_002850"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          170 a           86 c           88 g          114 t
ORIGIN      
        1 cgcttgcgaa gcttgattct atcgaaaaaa cttcaaaaaa gttcttgaca aataacaaac
       61 gacaagttat gataaatgag ttgctgtaag atttcgaaaa acgaaatcaa aaaaactttt
      121 aaaaaagagt tgacaattac ttgtctctct gatatgatat aaaagttgct gcggcagcca
      181 aaaacgaatc gggaaacact cctgatgaag tagacctttg aaaaatgaac aaagtaagac
      241 aaaccaaatg tgtagggcgt cttgattcaa ttcaagacaa caaacatttt taacaagcaa
      301 gcaatatgct agcaaacaaa ttgagcttaa caatcgcaag gttgttcgaa ctttttatga
      361 gagtttgatc ctggctcagg acgaacgctg gcggcgtgcc taatacatgc aagtcgaacg
      421 cttctttttc caccggagct tgctccaccg gaaaaaga
//
LOCUS       DAGCYL010000349          438 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.365, whole genome shotgun sequence.
ACCESSION   DAGCYL010000349 DAGCYL010000000
VERSION     DAGCYL010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.365"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(41..114)
                     /locus_tag="MB216_002851"
     tRNA            complement(41..114)
                     /locus_tag="MB216_002851"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78..80),aa:Ile,seq:cat)
BASE COUNT          154 a           72 c           83 g          129 t
ORIGIN      
        1 aaaaaattct aacacagaac gattcaattg gctacattaa tgtaccctgt aggactcgaa
       61 cctacgaccg gacggttatg agccgtctgc tctgaccaac tgagctaagg gtagtggttg
      121 ttgccacata aagccataaa caatcaacca gtagaatgcg tggcaacaaa cctgttatcg
      181 catatcttgg agtgtgacta tttatgagtg atagtgaaga tatgcgtctc catatatgta
      241 tggttgagac agataaacat gtatgaaaaa cgttgttaaa tcaacgtttt tttattttgg
      301 tcaaggagag aaaagtagag aaatacaaaa ttttttgcat tgattttgca ttcaaaaaaa
      361 cattcaaaca tttagaaatc cccaatacgt attcaggaaa atctatttta aggtttacaa
      421 aaagaacttt tgttcgta
//
LOCUS       DAGCYL010000350          436 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.366, whole genome shotgun sequence.
ACCESSION   DAGCYL010000350 DAGCYL010000000
VERSION     DAGCYL010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.366"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
BASE COUNT          127 a           76 c           58 g          175 t
ORIGIN      
        1 gaaattgtgc attatttctt cacatttatt aggttatagt ttaagaaagt ttaaacattg
       61 cgcaatttaa ttattttagc tgtttctttt cataaaaaaa catgttgtga ctctgagaca
      121 aatcatagtc catagtgatg tctctagagt aactacgcct aatattacga ggcatcatat
      181 taatctatca gcactagtac ctaaactagc atccctttcg aatatttttt cttatataat
      241 aaagtttgct ttcactcctc taatctcacc aaatcgactg gcttgcgttg tgtgatacta
      301 ggaactgatt ggtttagaac gacttttcta ttgtaatcat atttttcgcc atcctcgttg
      361 tcttctaatc cttcaatgaa tgatccctaa tgtgtgtatt aaataattta atttttttgt
      421 tcctatttat ttaaca
//
LOCUS       DAGCYL010000351          429 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.367, whole genome shotgun sequence.
ACCESSION   DAGCYL010000351 DAGCYL010000000
VERSION     DAGCYL010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.367"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            85..>429
                     /locus_tag="MB216_002852"
     CDS             85..>429
                     /locus_tag="MB216_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527599.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          191 a           58 c           63 g          117 t
ORIGIN      
        1 gtcaaaagat taaatatata aaaagccttc gggctttttc ttttaacaat taataggaac
       61 gtatattcga aaggaataga aataatgtgg ttagaaaaaa taaatgacaa aaaatttaag
      121 tacaccgaac gttatacgga tcccttaact gaaaaaaaga gaaaagtgtc cgttacttta
      181 tctagcaatt cacgccaagc ttggaatcaa gctaacttac ttttgaatga aaaaatcgca
      241 gaaaaaataa aacgaaatga agaattacca ctaacttttg gagaattaaa aactaaatgg
      301 gatgaaaaat acaagccaac tgtaaaagaa agttcctatc gaacaactca ggtttatcta
      361 tcattaattt caaaatatat aaaagatgat gtacttgtaa aaaacgttaa tagtaatctt
      421 atacaagat
//
LOCUS       DAGCYL010000352          403 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.368, whole genome shotgun sequence.
ACCESSION   DAGCYL010000352 DAGCYL010000000
VERSION     DAGCYL010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.368"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>403)
                     /locus_tag="MB216_002853"
     CDS             complement(<1..>403)
                     /locus_tag="MB216_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBT4527600.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNK"
BASE COUNT          128 a           77 c           69 g          129 t
ORIGIN      
        1 ttttattgaa catatatctt actttatcta tccgactatt tagacgacgg gtctggcaaa
       61 caggttcgcc agtggtaacc tgatatcctt ttagctctgc taaacaaaca ctaagcccat
      121 ttgtaaaaaa agttaaatca ttgcgataat cttgaataca tcgagcagga atttctccaa
      181 taataatgac ctcattattt ttcagttgag tatttacgat atttgcacaa tatttgggag
      241 catcgttata tgcccgtgaa agatattcct gtggtgcata aactttaaaa ctaagatatg
      301 gctctaacaa ttctgttcca gcttttctaa aggcttgctc cagtacaata ggagtaagca
      361 tccgaaaatc tgctggagta ctaacagggc tatagtataa acc
//
LOCUS       DAGCYL010000353          399 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.369, whole genome shotgun sequence.
ACCESSION   DAGCYL010000353 DAGCYL010000000
VERSION     DAGCYL010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.369"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..352)
                     /locus_tag="MB216_002854"
     CDS             complement(<1..352)
                     /locus_tag="MB216_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527601.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKCFESAVVKFPQFKLNP
                     AASIQFQGHVFDVLA"
BASE COUNT          138 a           72 c           40 g          149 t
ORIGIN      
        1 tagctaaaac atcaaataca tgtccttgaa attgtatcga ggcagctgga tttagtttaa
       61 attgaggaaa ctttaccaca gctgattcaa aacacttgtc acattttttt atttttttac
      121 ctttgtaaaa atattcgatt gaatattcta tttggcacga ctcacttggc agtatatgta
      181 tgtcaatatt tttcatctgt ttcataggta tttcaaaatc taaactatta aaaacatata
      241 attccctgta attatttaca tctaactcta ctaaatttat ctttggaaca atagatcctc
      301 cacccattcc accataataa aaaaaatctt gttctctatc aaaatagcac atattaagct
      361 cagtttgttt atcatcctca ataattttat aaatcaaac
//
LOCUS       DAGCYL010000354          390 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.370, whole genome shotgun sequence.
ACCESSION   DAGCYL010000354 DAGCYL010000000
VERSION     DAGCYL010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.370"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>390
                     /locus_tag="MB216_002855"
     CDS             <1..>390
                     /locus_tag="MB216_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HBT4527602.1"
                     /translation="SRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKP
                     VRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLET
                     LSEEANLPNILGDKGYISHKIHEKLAL"
BASE COUNT          138 a           74 c           63 g          115 t
ORIGIN      
        1 gagtcgattc actcgcttaa gttcaaactt agcttacact atcaagatta ttcgatactt
       61 tttcatcaag aagctcacca aaggtgaact agtcggaatt atagatagtt ttcctagtcc
      121 attatgtaaa ccagttagaa atagacaagc aaaactattg aatcaaatag ccaaagttgg
      181 ctataactca acaaaaaaat cttattttta tggtcttaaa attcatatga tcgttactaa
      241 gactggcttt ccgattactt actcaatcac gaatccaggt gtccatgacg tgaaagtgtt
      301 agagacttta tcggaagaag cgaatcttcc caatatccta ggggacaaag gatatattag
      361 ccataaaatc catgaaaagc ttgcccttaa
//
LOCUS       DAGCYL010000355          388 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.371, whole genome shotgun sequence.
ACCESSION   DAGCYL010000355 DAGCYL010000000
VERSION     DAGCYL010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.371"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            106..388
                     /locus_tag="MB216_002856"
                     /pseudo
     CDS             106..388
                     /locus_tag="MB216_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
BASE COUNT          125 a           66 c           62 g          135 t
ORIGIN      
        1 ttttacactc tcatcaaatc attagaatcg tgataagaca ttgtattaaa tctaatcctt
       61 tatccatttt ttatcaatct tacttcgcac catctccaat acttattatt agcctctctt
      121 ggagcatgta aagttattgt agctcggtct ttcgctaaag tgcagggcat aaatttaaac
      181 gatatccgtg tagagataga tggagtatta gatcctgatg gttttatagg aaaaaataaa
      241 gcagctaaaa taggctattc tgcaatcgta tctaaatttt atattgaagc tgataatact
      301 gatgaagaga tttcagattt tatcgcattt attgatcgta cttgtccggt agctgatacc
      361 attaaaaatg cacctacgac taaatcag
//
LOCUS       DAGCYL010000356          370 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.372, whole genome shotgun sequence.
ACCESSION   DAGCYL010000356 DAGCYL010000000
VERSION     DAGCYL010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.372"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(152..>370)
                     /locus_tag="MB216_002857"
     CDS             complement(152..>370)
                     /locus_tag="MB216_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4527603.1"
                     /translation="KNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSH
                     FSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          135 a           69 c           41 g          125 t
ORIGIN      
        1 ttaacacact attattacca ttacttagac gctcagattc tacgaaacaa ctcaaatcag
       61 aaatttccca aaaaattttt tgttaaaatc caatttcctc aactcctcca aactacgtaa
      121 atgatatagt ctcataataa aataaaacct ctcagctaat gttgttgaaa ttatccacta
      181 aacctaattg atattgctta ggagatacac ctgcgtactt tttgaatact ctcgaaaagt
      241 gtgaagaatc tgaaaatcct aaacctaaag aaacttcact gatagtatgg tttgatttca
      301 acattatttt agccgtagaa atttttcgct tagagatata attataaatc gtaatatgca
      361 tttcattctt
//
LOCUS       DAGCYL010000357          358 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.373, whole genome shotgun sequence.
ACCESSION   DAGCYL010000357 DAGCYL010000000
VERSION     DAGCYL010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.373"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            180..>358
                     /locus_tag="MB216_002858"
     CDS             180..>358
                     /locus_tag="MB216_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527604.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTG"
BASE COUNT          122 a           58 c           68 g          110 t
ORIGIN      
        1 gatatagtgc gcggctactt ctgccatgat tgcaaaaaca aaatcaaaaa gaattttcaa
       61 acctataacc tcctcccctt gccagattag gccggggtag cagacagcaa gactattata
      121 gcataattga aatatccaaa tgattggatt tttgctataa ttttagcgag gtgattgtga
      181 tgaacaaata tgttggtttg cttgataaaa tccgagtaat aaaaacgcaa ccgttattgg
      241 ttcgctttac tttacaaacg attcatgaat cgattaattg tgtagttgca gatattgaaa
      301 ttatcgataa attgctaatt atggatgatg gaaaatacaa tattgccgtc acaggtca
//
LOCUS       DAGCYL010000358          333 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.374, whole genome shotgun sequence.
ACCESSION   DAGCYL010000358 DAGCYL010000000
VERSION     DAGCYL010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.374"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>333)
                     /locus_tag="MB216_002859"
     CDS             complement(<1..>333)
                     /locus_tag="MB216_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4527605.1"
                     /translation="ELAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLL
                     RQDPVFKAVLDRKELASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELII
                     DLDSTHSDT"
BASE COUNT          101 a           56 c           72 g          104 t
ORIGIN      
        1 tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc gtatcattac ggatgaggcg
       61 tgctttatcg ataagttctt ggttcaaagc ttgaagttca tggatattct cctcagatag
      121 tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt tctttcctat ctaaaacagc
      181 tttaaacaca ggatcttgtc tcaggagatt agctgcagaa tcagctgagt aaccagcaat
      241 taattgcata atgaactgct caagtatgga taagttgtca tgagtaaaat acgctcgttc
      301 gtcttcaatg tgaatgtgtt gcttagccag ttc
//
LOCUS       DAGCYL010000359          332 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.375, whole genome shotgun sequence.
ACCESSION   DAGCYL010000359 DAGCYL010000000
VERSION     DAGCYL010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.375"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>332
                     /locus_tag="MB216_002860"
     CDS             <1..>332
                     /locus_tag="MB216_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527606.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DAGCYL010000360          322 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.376, whole genome shotgun sequence.
ACCESSION   DAGCYL010000360 DAGCYL010000000
VERSION     DAGCYL010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.376"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(44..>322)
                     /locus_tag="MB216_002861"
     CDS             complement(44..>322)
                     /locus_tag="MB216_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4527607.1"
                     /translation="GITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLE
                     LFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           87 a           81 c           60 g           94 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt cc
//
LOCUS       DAGCYL010000361          322 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.377, whole genome shotgun sequence.
ACCESSION   DAGCYL010000361 DAGCYL010000000
VERSION     DAGCYL010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.377"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            39..>322
                     /locus_tag="MB216_002862"
     CDS             39..>322
                     /locus_tag="MB216_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBT4527608.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQF"
BASE COUNT          131 a           40 c           62 g           89 t
ORIGIN      
        1 gaaaattaca caaaaatact ttatgctgag gtttcaaaat gacaaatatc ttcgaaaaaa
       61 tcgtaaatga tttaaattta gaacaaaaag atgttgtgga aagtgaagat aacctttatg
      121 taaatgcatg tcctgggagt gggaaaacaa gggtacttac aaggaaaatt gcttaccaag
      181 caattaaaca cgatggatca ctaaaaagaa taattgctat cacatacact aatagagctg
      241 ctgaagaaat taaggaacgc ctaagtttac ttagtataga tgatgatatt aatatttggg
      301 tggggacaat acatcagttt tg
//
LOCUS       DAGCYL010000362          315 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.378, whole genome shotgun sequence.
ACCESSION   DAGCYL010000362 DAGCYL010000000
VERSION     DAGCYL010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.378"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
BASE COUNT          114 a           57 c           76 g           68 t
ORIGIN      
        1 taaaatgact gacggatatt gaatagtaat aaataacaat aattcagacg gatacagtga
       61 gtgaagtagc ttgctacgaa cgaacaggca gaatgaaacg gtagtttcat tagagcaagt
      121 cgcttgcgac gtagcgttac cactgatgta actggcaagt tacatagaga gagtttgctt
      181 gcaaacgaac gttacccact tgaatagaaa acaaaaataa catattagtg aattttgaat
      241 aggagccaca actcaaccaa ccgcttgcgg tgggtgagtc ccacggagga aaagacgaac
      301 gaacggtcgc gtagc
//
LOCUS       DAGCYL010000363          285 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.379, whole genome shotgun sequence.
ACCESSION   DAGCYL010000363 DAGCYL010000000
VERSION     DAGCYL010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.379"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>285)
                     /locus_tag="MB216_002863"
     CDS             complement(<1..>285)
                     /locus_tag="MB216_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527609.1"
                     /translation="KVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALILDLIDKLSD
                     LANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYEYE"
BASE COUNT           91 a           48 c           33 g          113 t
ORIGIN      
        1 ctcatattca tagctatcaa ttagctcagt tgaactataa gctttttcta gtagttccat
       61 ttcccatttt ggagagtttg gatcactttt tcttagtcta gctatatctt ctaaaatttt
      121 cttagaagta ttatcctcgt aaatatttgc taaatcagat aatttatcaa ttaaatctaa
      181 aataagcgca ctgtaattga cagctattgc agaacgataa tttccctgat aaaaactact
      241 tctaacttct tctaataatg gaataattct aggattttta acttt
//
LOCUS       DAGCYL010000364          268 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.380, whole genome shotgun sequence.
ACCESSION   DAGCYL010000364 DAGCYL010000000
VERSION     DAGCYL010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.380"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..132
                     /locus_tag="MB216_002864"
     CDS             <1..132
                     /locus_tag="MB216_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4527610.1"
                     /translation="RRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            189..>268
                     /locus_tag="MB216_002865"
     CDS             189..>268
                     /locus_tag="MB216_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4527611.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSS"
BASE COUNT           93 a           37 c           48 g           90 t
ORIGIN      
        1 cgcagaagag aaaagattat tcgcatcttc cccaatcaaa catcagccaa tcgcttaatt
       61 ggagccgttc ttatggacct acatgatgaa tggatttatt cttcaagaaa atacatcaat
      121 tttgataagt agaaatggta aaaacattgt atagcatttt acacaggagt ctggacttga
      181 ctgagtttat ggaagaagtt ttaattgatg ataatatggt ttttgatatt gataatttaa
      241 aaggatttct taatgatacc agttcatt
//
LOCUS       DAGCYL010000365          265 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.381, whole genome shotgun sequence.
ACCESSION   DAGCYL010000365 DAGCYL010000000
VERSION     DAGCYL010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.381"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            135..>265
                     /locus_tag="MB216_002866"
     CDS             135..>265
                     /locus_tag="MB216_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBT4527612.1"
                     /translation="MEEVCNMSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKH"
BASE COUNT           92 a           46 c           44 g           83 t
ORIGIN      
        1 actaaaaaaa catttttgaa aataaacatc agcagtccta tcaatgcatg tgagcttata
       61 gaagttattc gcagcgcagc ataattgcat tagcttcttt tcgtgttttt tgcctctttt
      121 ccctttaaat cttaatggag gaggtgtgca atatgtcaga taacgacgaa cgcgtaaatg
      181 cccagttgat tcgattcttt gaaaaggtca ttttgaacac cgctaatact tattataaaa
      241 aacaagaaga aattcaaaaa catga
//
LOCUS       DAGCYL010000366          261 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.382, whole genome shotgun sequence.
ACCESSION   DAGCYL010000366 DAGCYL010000000
VERSION     DAGCYL010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.382"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
BASE COUNT           97 a           36 c           45 g           83 t
ORIGIN      
        1 ttagtaaatt caaatatatg gtatgcttct tagtgaattc aaatataaaa gagtttaaag
       61 cgtaatacac ttatggggaa gtggtttggg gtgcgcttta aactcttctt tattattatc
      121 tcacagcatg cttaaaaggt ctatggatta agttatgtaa aaaagtgcct ctttaaacgt
      181 cctaaaatcc aaacaaggat agcataatgt acactatcgt tgatccagaa aacaggtaaa
      241 taaattttgc aaaagcaata a
//
LOCUS       DAGCYL010000367          257 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.384, whole genome shotgun sequence.
ACCESSION   DAGCYL010000367 DAGCYL010000000
VERSION     DAGCYL010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.384"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>257
                     /locus_tag="MB216_002867"
     CDS             <1..>257
                     /locus_tag="MB216_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HBT4527613.1"
                     /translation="KAGFVTEDNLYLDSTHIKANANKHKFTKEMTHDEAKAYQDELED
                     EINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKG"
BASE COUNT          103 a           42 c           58 g           54 t
ORIGIN      
        1 aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat caaagctaac
       61 gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc ctatcaggat
      121 gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc ttttactttg
      181 gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga tccggaaagc
      241 ggctactacg tcaaagg
//
LOCUS       DAGCYL010000368          248 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.385, whole genome shotgun sequence.
ACCESSION   DAGCYL010000368 DAGCYL010000000
VERSION     DAGCYL010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.385"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            21..218
                     /locus_tag="MB216_002868"
     CDS             21..218
                     /locus_tag="MB216_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBT4527614.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT           89 a           28 c           57 g           74 t
ORIGIN      
        1 aatttagggg tgattagatt atgaaacatt taaaaatttt gtctattaaa gagacacaac
       61 ttatctatgg gggtaccact catagtggaa aatattatgg aaatggagtg tattgcacta
      121 aaaataaatg tacggtcgat tgggccaagg caactacttg tattgcagga atgtctatag
      181 gtggtttttt aggtggagca attccaggga agtgctaaaa tgaaaaaaaa tgctaagcaa
      241 attgttca
//
LOCUS       DAGCYL010000369          247 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.386, whole genome shotgun sequence.
ACCESSION   DAGCYL010000369 DAGCYL010000000
VERSION     DAGCYL010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.386"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>247
                     /locus_tag="MB216_002869"
     CDS             <1..>247
                     /locus_tag="MB216_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4527615.1"
                     /translation="YGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYN
                     HSLPNTDILVRGDSGFATPGVYDSCESKKSHYVIRLK"
BASE COUNT           74 a           49 c           51 g           73 t
ORIGIN      
        1 cctatggtta ccatccattg gtagcttttg acggattgac tggtgacttc ttaaaagctg
       61 aactacgttc aggaaatcaa tatacatcaa aaggcgtaaa ggagtttctc acacctttat
      121 tagagcacta taatcactct ctaccaaaca ctgacatctt ggttcgtgga gacagcgggt
      181 tcgctacacc tggtgtgtat gattcgtgtg aatcaaaaaa gagtcattat gttattcgac
      241 tgaagaa
//
LOCUS       DAGCYL010000370          242 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.denovo.387, whole genome shotgun sequence.
ACCESSION   DAGCYL010000370 DAGCYL010000000
VERSION     DAGCYL010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.denovo.387"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            80..>242
                     /locus_tag="MB216_002870"
     CDS             80..>242
                     /locus_tag="MB216_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4527616.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVD"
BASE COUNT           83 a           39 c           47 g           73 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcacgacaa aataaacaac
       61 tgagaggacg atatgctata tgaatgattc tatcaaaaaa atgctgcgat taatagagaa
      121 agatttgatg attacagagg tctcttacga gacctttcag aagaaaaaga cgttgatcgt
      181 cgatgctgtt ttctcacctg ctccccatac ctgtagaaat tgtggttcta ctgtggtaga
      241 tg
//
LOCUS       DAGCYL010000371        20862 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.guided.029, whole genome shotgun sequence.
ACCESSION   DAGCYL010000371 DAGCYL010000000
VERSION     DAGCYL010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.guided.029"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            267..1304
                     /locus_tag="MB216_002871"
     CDS             267..1304
                     /locus_tag="MB216_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBT4527617.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="MB216_002872"
     CDS             complement(1393..1716)
                     /locus_tag="MB216_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBT4527618.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="MB216_002873"
     CDS             complement(1703..2410)
                     /locus_tag="MB216_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBT4527619.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="MB216_002874"
     CDS             2496..2837
                     /locus_tag="MB216_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBT4527620.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="MB216_002875"
     CDS             complement(3008..3856)
                     /locus_tag="MB216_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527621.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="MB216_002876"
     CDS             4454..5317
                     /locus_tag="MB216_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBT4527622.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="MB216_002877"
     CDS             5417..5899
                     /locus_tag="MB216_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBT4527623.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="MB216_002878"
     CDS             complement(6162..6629)
                     /locus_tag="MB216_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBT4527624.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="MB216_002879"
     CDS             6752..7603
                     /locus_tag="MB216_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4527625.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="MB216_002880"
     CDS             complement(7854..9812)
                     /locus_tag="MB216_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4527626.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="MB216_002881"
     CDS             complement(9809..10681)
                     /locus_tag="MB216_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4527627.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="MB216_002882"
     CDS             complement(11023..11895)
                     /locus_tag="MB216_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4527628.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="MB216_002883"
     CDS             12023..12592
                     /locus_tag="MB216_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBT4527629.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="MB216_002884"
     CDS             12659..13396
                     /locus_tag="MB216_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4527630.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="MB216_002885"
     CDS             13627..14895
                     /locus_tag="MB216_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBT4527631.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="MB216_002886"
     CDS             15382..15846
                     /locus_tag="MB216_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4527632.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="MB216_002887"
     CDS             15830..16459
                     /locus_tag="MB216_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBT4527633.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr(C)"
                     /locus_tag="MB216_002888"
     CDS             16813..18291
                     /gene="msr(C)"
                     /locus_tag="MB216_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBT4527634.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="MB216_002889"
     CDS             18338..18784
                     /locus_tag="MB216_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBT4527635.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="MB216_002890"
     CDS             complement(18983..19498)
                     /locus_tag="MB216_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4527636.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20816
                     /locus_tag="MB216_002891"
                     /pseudo
     CDS             19615..20816
                     /locus_tag="MB216_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         6631 a         3703 c         4315 g         6213 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt atggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtgta ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaca ttgatgacag gattgtttat catcgttgta ttcgctggtc
    19921 ttggcggatt atctgggaat atcggaatgc tgattgggtt acgtgccggc tggggtgtcg
    19981 ggaatgcttt atttgtctct actgctttag caacgatcgt cagtatttta gttggtaaca
    20041 cagaaaaagc aatcatgatg tatgaagcag cacttggctg cggaatggct gttggccctc
    20101 tagtcggtgg ggtcttgggc agtggttcat ggcgttatcc attttttggg gtagcatttt
    20161 tgatgttttg cggttttata gccctagcgg tacttttacc tgaaactgag aaaccaaaga
    20221 gaaaagtagg cctttgggaa ggtgtcaaag cgttaagcaa tcataaactt cgttcagttg
    20281 gactaatcgc attgttatat aatttcggat tctttacttt acttgcgtat gcgccatttt
    20341 tattgattgg ctattctgaa ttagaagtcg gttttgtatt ttttggctgg ggtgtgcttc
    20401 tggctattgc ttcgattttc gttgcaccgg ctcttgagcg gaagtttacg acttatcgga
    20461 caattttagc agcatttgtc ttattcatta tttgtttgct gttattagga gctggaactt
    20521 ctatgccagt ccttgtaccg atcagcattg tgttagcagg atttttccaa ggaattatca
    20581 atacactgtt gacgacgatt gctatggaaa taccaggtct gcaacgaaac gttgcttcgt
    20641 ccagctatag ttttgtccgc tttttcggtg gagctttagc accatttatt gcaggtgtga
    20701 ttggtgaaca tattggtgaa agctatgcgt tttattttgc aggtgccatt gttcttatca
    20761 gtgtatgttg tatcatttat caccgtaatt attttgctac aaatgaagaa gaataagaaa
    20821 tcagaggttg tgaaaaagct gtcctgcatc aagtcataag aa
//
LOCUS       DAGCYL010000372         9215 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.guided.111, whole genome shotgun sequence.
ACCESSION   DAGCYL010000372 DAGCYL010000000
VERSION     DAGCYL010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.guided.111"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(134..577)
                     /locus_tag="MB216_002892"
     CDS             complement(134..577)
                     /locus_tag="MB216_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBT4527637.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="MB216_002893"
     CDS             complement(795..2135)
                     /locus_tag="MB216_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4527638.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="MB216_002894"
     CDS             2404..3300
                     /locus_tag="MB216_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBT4527639.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="MB216_002895"
     CDS             complement(3450..4391)
                     /locus_tag="MB216_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBT4527640.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="MB216_002896"
     CDS             4517..5638
                     /locus_tag="MB216_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBT4527641.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYSLGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="MB216_002897"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="MB216_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBT4527642.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="MB216_002898"
     CDS             7426..7992
                     /locus_tag="MB216_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBT4527643.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..>9215
                     /locus_tag="MB216_002899"
     CDS             8203..>9215
                     /locus_tag="MB216_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBT4527644.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LIN"
BASE COUNT         3074 a         1698 c         1839 g         2604 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttattcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aacca
//
LOCUS       DAGCYL010000373         5732 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.guided.166, whole genome shotgun sequence.
ACCESSION   DAGCYL010000373 DAGCYL010000000
VERSION     DAGCYL010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.guided.166"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(243..614)
                     /locus_tag="MB216_002900"
     CDS             complement(243..614)
                     /locus_tag="MB216_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4527645.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            759..944
                     /locus_tag="MB216_002901"
     CDS             759..944
                     /locus_tag="MB216_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBT4527646.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            1102..1269
                     /locus_tag="MB216_002902"
     CDS             1102..1269
                     /locus_tag="MB216_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527647.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            1887..2546
                     /gene="vanR"
                     /locus_tag="MB216_002903"
     CDS             1887..2546
                     /gene="vanR"
                     /locus_tag="MB216_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HBT4527648.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDLEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            2549..3892
                     /gene="vanS"
                     /locus_tag="MB216_002904"
     CDS             2549..3892
                     /gene="vanS"
                     /locus_tag="MB216_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBT4527649.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            4063..4869
                     /gene="vanY"
                     /locus_tag="MB216_002905"
     CDS             4063..4869
                     /gene="vanY"
                     /locus_tag="MB216_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBT4527650.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            4882..5718
                     /gene="vanW"
                     /locus_tag="MB216_002906"
                     /pseudo
     CDS             4882..5718
                     /gene="vanW"
                     /locus_tag="MB216_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT         1682 a         1197 c         1418 g         1435 t
ORIGIN      
        1 ccattcaggg gtttggggat ttccccaaca agcaaaacgg acgcggcccg gcagggacgg
       61 ggcaggccgt ttttccggga gtggctacac tccctatgct tgctacgtta ctgtttctcc
      121 actaacaaga cgtttccaaa gccccaaact acccgcaaaa tagccctctg gaaagaaaaa
      181 agtgcaaaaa aatagaagct acgctgtgca gctcccgcta aaagtaaaac agaggatgcc
      241 agttacacat cctccgtttc attcttcttc ggcttttcaa ttagtatggc gctgtcaatc
      301 gcgccctcga tgatcggcag gtattcttcg gggcagagtt tcagcttgtg gctgaccctt
      361 tgccgcagtt cgctttcctc ccgcatgatc tccgggttaa agtagcgttc cacaggaagc
      421 ccgcaggctt taataagctg tatgacaacc ggcagactgg gaatggcccc gtcgttttcg
      481 atgttggcaa gatagcgcca gtcaatattt attttctctg ccattgcttt gcgcgaaacg
      541 cgctttgctt gccgggccgc tttcacatcc gcgccgaaag tttcaaagcc gggacaatct
      601 tcaatattcg ccatatcgca cctcccactt acattgtata tttcatactc tgcccgtgga
      661 atgttggtat atgcaccttt cttaaagttt atatttcaca ttttgtttct tttattcttc
      721 gagcaaaaga attataatgt accctgtgga ggtgtactat ggactacatg acgttgaaag
      781 aggcaggcga gaaatggggt gtgcccccac gatggataaa ctactattgt gctgcgggac
      841 gcatccccgg cgctgtaaag atggcgacga tctggctcat tcccaaaact gcggaaaagc
      901 cgattgacag gcgtacaaag aaaggaaagg cggttactga atagtataat gaaaccggcg
      961 cattttggat atggttttga agagagagaa agcatggtat tttgtcacgg aaaaactata
     1021 agatgataga tggcaggctg ctccagaccg ataaaaagta ttctcatctg aaacagaaag
     1081 aaaagattta cacatggatg tatgaagaag cgaaacttta tcatgacaga aatggaaaat
     1141 gcccgaaaca aagacgagga agctgctgct gtatcagccg tatatggtag aattgaaaag
     1201 gccggcatct ggattcttta tggtgaagtc tccagacatt ggataactgc tggtctaatg
     1261 cgcctttgag tatggggtga ttgattttaa taccggggag gtacggcttt ttgaggaaaa
     1321 tattctggga tttacatttg gcgattatta catttcctgt aactgcaagg acacggatga
     1381 tgaagatatg aggaatatag acttgctgat tttctactgt cttgaacggt attaggactg
     1441 aatctgctgc gcggagtgcg gcaagagcga ccggagggag tacacacagg ccctgcctgt
     1501 gtgaaaagcc ataccgcaag gtgcattcgc aaaaaagagg ccgcgcattt tgctgaggct
     1561 tggggtgtcc ccaacaagca aaacgagaac gccgcaggcg ggcaagtggt ggatcagaat
     1621 atccgtaaag gatattctga tccattgctt gccagtatta tcgggatgtt ttgacaaccg
     1681 ctgtaatcga aacatcccaa acattaagaa ggtgttaatt attattatct tgatttaatt
     1741 cgtttaaacc aataatatag atacatacac catgaaaaga agcgagtatc ccaatgacgg
     1801 atgattaaaa tccgaaaaga aatcgctttg aaactacagg gaaactacag actgttatgt
     1861 tatcttctta aaatggaggg atttttatgt cgatacgaat tctacttgtc gaggatgatg
     1921 atcatatctg taatacagta agggcgtttc tggctgaggc aggatatcag gtggatgcct
     1981 gcacagatgg aaatgaggca tacaccaagt tttacgaaaa cacttatcaa ctggttattc
     2041 ttgatattat gctgcccggt atgaacgggc atgaactttt gcgtgaattt cgtgcgaaaa
     2101 atgatactcc cattctgatg atgacagccc tgtcggatga cgaaaaccaa atccgggcgt
     2161 ttgatctaga ggcagacgac tatgtaacaa agccattcaa gatgcagatt ttactaaagc
     2221 gggtggaagc cctgctgcgg cgcagcggtg cgctggcaaa ggaaatccgt gtcggcaggc
     2281 tgacacttct gccggaggat ttcacggtac tttgtgacgg tacagagctg cccctgacac
     2341 gaaaagaatt tgaaatcctt ttgctgctgg tgcagaacaa aggcagaacc ttaacgcatg
     2401 aaatcatttt gtcacgtata tggggatatg actttgagag tgatggcagc acagtccaca
     2461 ctcatatcaa aaatttgcgg gccaagctgc cggaaaatat catcaaaacc attcgcggtg
     2521 taggataccg attggaggaa tcataataat ggaaagaaaa gggattttca ttaaggtttt
     2581 ttcctatacg atcattgtcc tgctactgct tgtcggtgta acagcaacac tgtttgcaca
     2641 gcaatttgtg tcttatttca gagtgatgga attacagcaa acagtaaaat cctatcagcc
     2701 attggtggaa ctgattcaga atagcgatag gcttgatatt caagaggtgg cagggctgtt
     2761 tcactacaat aaccaatcct ttgagtttta tattgaagat aaagagggaa gcgtactcta
     2821 tgccacacca aatgccaata catcaaatag ttttagaccc gactttcttt atgtggtaca
     2881 tagagatgat aatatttcga ttgttgctca aagcaaggca ggtgtgggat tgctttatca
     2941 agggctgaca attcggggaa ttgttatgat tgcgataatg gttgtattca gccttttatg
     3001 cgcgtatatc tttgcgcggc aaatgacaac gccgatcaaa gccttagcgg acagtgcgaa
     3061 taaaatggca aacctgaaag atgtaccgcc gccgctggag cgaaaggatg agcttggcgc
     3121 actggctcat gatatgcatt ccatgtatgt caggctgaaa gaaaccatcg caaggctgga
     3181 ggatgaaatc gcaagggaac atgagttgga ggaaacacag cgatatttct ttgcggcagc
     3241 ttctcatgag ctaaaaacgc ccatcgcggc tacaagcgtt ctgttggagg gaatgcttga
     3301 aaatatcggt gactacaaag atcattctaa gtatctgcgc gaatgcatca aaatgatgga
     3361 taggcagggc aaaatcattt ccgaaatact ggagcttgtc agcctgaatg atgggagaat
     3421 cgtacccata gctgaaccgt tggacatagg gcgcacggtt gccgagttgc tgcccgattt
     3481 tcaaaccttg gcagaggcaa acaaccagcg gttcgtcaca gatattccag ccgggcaaat
     3541 tgtcctgtcc gatccgaggc tgctccaaaa ggcactatcc aatgtcatat tgaatgcagt
     3601 tcagaacacg ccgcagggag gcgaggtacg gatatggagt gagcctggtg ctgaaaaatg
     3661 ccgccttttt gttttgaaca tgggcgttca cattgatgat actgcgcttc caaggctgtt
     3721 caccccattc tatcgcattg atcaggcgcg aagcagaaaa agtgggcgaa acggtttagg
     3781 acttgccatc gtacaaaaaa cgctggatgc catgagcctc cagtatgcgc tggaaaacac
     3841 ctcggatggc gttttgttct ggctggattt accgctcaca tcaacattgt aaatatttaa
     3901 aacttaaatg attttgaccg acaggtataa ccctgccggt ctttttttgc tgctacagga
     3961 aaactacaga ctggctacag ggaaagtaca gatgcgcttg ccataataac aatcgtacca
     4021 gccacaaatc gtagttttat tgcaaaggag gcattcgatc aaatggaaaa aagcaactat
     4081 cattccaatg cggatcatca caaacggcat atgaaacaat ccgtggaaaa gcgggctttt
     4141 ctatgtgcgt tcattatttc gttcacagtc tgcacgctgt ttttggggcg gagactggct
     4201 tccgtattgg aggcaacaca gataccgccc atccctgcaa ctcatacagg cagcagcact
     4261 gacgtagtgg agaatttgga ggaaaacgct cttgccaccg ccaaagaaca gggagatgaa
     4321 caggaatgga gcctgatttt agtgaacagg cagaacccca tccccgcaca gtacgatgtg
     4381 gagcttgagc aactatcaaa tggtgagcgg atagatattc ggatttctcc ctatcttcaa
     4441 gatttgtttg atgccgcaag aactgatgga gtttacccga ttgtcgcatc cggataccga
     4501 acaacagaaa aacagcaaga aattatggat gaaaaaattg ccgaatataa ggcgaaaggc
     4561 tacacctctg cacaggctaa agcggaagca gaaacttggg tggccgtgcc gggaacgagc
     4621 gagcatcagc ttggtcttgc tgtggatatc aatgcggacg gaattcattc aacaggcaac
     4681 gaggtttata gatggctgga tgaaaacagc tatcgttttg gttttattcg tcgctaccca
     4741 ccagacaaga cagagataac cggtgtgagc aacgagccgt ggcattaccg atatgttggc
     4801 atcgaagctg ccacagagat gtacaaccaa ggggtttgcc ttgaggaata tttaaaacca
     4861 gaaaaatgag aaaaggatgt tatggacaga aaaagattga cacagcgttt cccgttcctg
     4921 cttccaatga gacgggcgca gagaaaaatg tgcttttatg cgggaatgag atttgacggc
     4981 tgtcgctatg cgcagacgat aggggaaaaa tcgctttccc atttgctctt tgaaacggat
     5041 tgcgcattat acaaccacaa taccggattt gacatgatat accaggaaaa caaggtgttc
     5101 aacttaaagc tggcagcaaa gaccttaaac ggcttattga taagaccggg ggaaaccttt
     5161 tctttctggt ggctggtacg ccatgcggac aaagataccc cctataaaga cggccttacg
     5221 gtgaccaatg gtaaactcac caccatgtcg ggcggcggta tgtgccagat gagcaattta
     5281 ctattttgga tgttcctgca tacgccattg acaattatcc agcgcagagg tcacgaagta
     5341 aaggagtttc cagagccaaa cagtgacgag attaaagggg tggatgcaac catttcagag
     5401 ggctggattg atttaaaagt gcgaaacgat accgactgca cctaccaaat atgagtgacc
     5461 ttagatgatg agaaaatcat cggtcaggtg tccgccgaca aagagcccca agcattatac
     5521 aaaattacaa atggcagtat ccagtatgtc cgtgaaagtg gcgggattta tgaatatgcc
     5581 caggttaaac ggatgcaagt tgccttaggt accggggaaa taatagattg caagctgctt
     5641 tatacaaaca aatgcaaaat ctgttatccc ctcccggaaa gtgtggatat tcagtctaat
     5701 ggtgataagt caagatgaaa tttaagattt tc
//
LOCUS       DAGCYL010000374         3604 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.guided.204, whole genome shotgun sequence.
ACCESSION   DAGCYL010000374 DAGCYL010000000
VERSION     DAGCYL010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.guided.204"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..164)
                     /locus_tag="MB216_002907"
     CDS             complement(<1..164)
                     /locus_tag="MB216_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBT4527651.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELC"
     gene            394..891
                     /gene="dfrG"
                     /locus_tag="MB216_002908"
     CDS             394..891
                     /gene="dfrG"
                     /locus_tag="MB216_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBT4527652.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(963..2915)
                     /locus_tag="MB216_002909"
     CDS             complement(963..2915)
                     /locus_tag="MB216_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBT4527653.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2922..3158)
                     /locus_tag="MB216_002910"
     CDS             complement(2922..3158)
                     /locus_tag="MB216_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527654.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3393..>3604)
                     /locus_tag="MB216_002911"
     CDS             complement(3393..>3604)
                     /locus_tag="MB216_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBT4527655.1"
                     /translation="ELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSST
                     HRGPSELCGNVFKIEYTKKRQRLAY"
BASE COUNT         1115 a          622 c          583 g         1284 t
ORIGIN      
        1 ttacataatt caccatttat tgaagtatac atttctgtaa cttttatttt ttctttttct
       61 gatactctaa ccgaatctcg taaatgtagt actccactat aaaggcgtat atatgcaagt
      121 aagtagttca gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt
      181 cattttaact tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga
      241 taattgaatg aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt
      301 gcttattcaa ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta
      361 gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg
      421 gataagaata gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg
      481 gaatatgtta aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa
      541 tcaatcggaa gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt
      601 acctttaatg gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac
      661 gaagaagaaa tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt
      721 gagaaaatgt acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa
      781 gtgaattatg aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat
      841 ccgtataact actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgcgt
      901 taattgaaga agaacagtaa agataaagca caagagcttg ggattactcc aagctctttt
      961 tgtcatttga aataaattat atctttccaa tcaaattgtt tatctgtaat ccctaatctt
     1021 tgagcaggtg tcaaaacctt tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa
     1081 gttcttagaa ttgtaatcgc aaattgagca tacttcggat tgaagttagc gtaaatatag
     1141 ctcttcttat caccacgagc tgttgttaat ggtctttcaa gagatgatat ataacgtctt
     1201 atctgttgaa taaagctatt ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt
     1261 tcgtttggtt ccagagcaga taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc
     1321 ttagtggcta atggatgttt gatagggtta tcggcatatt tgcgatatct ttctccatct
     1381 ttactaatag cctcttcgtg gaattgatga cttgttagta attcagttag ataatgagat
     1441 gccaaagtcc gtagaaactt tgttttgaat cctctgatat ctccccaatc gagcagttct
     1501 gcttttgctt gtccgaattc ttttaagcat tgttttctgc ttttcgtctt gttaacttgg
     1561 caaataaaat gatgtgcatc gctcaacctt aattctttcg tgaatactct ataaaaggcg
     1621 tttctaatag agaagtcatc atcactaatc attcgccatt cgtcggcatt aaccatttgt
     1681 ttaattaacc aatagtgagc agttgtagta taaggagcat caacatttaa accatcgaca
     1741 aatctactcc tattagtaat tttccctaat tcgtgaagat aagcattcct attttcgcta
     1801 tcgttagcgg atggttcttg tggataataa gaccaatcta acctatcatt tttacgacta
     1861 aaagtattga gatggtcttc tttgaatttt cttgtatctt catttaattc atccattgaa
     1921 atattccaat cataggcaac atcagaacgg aacacatatc ttgataagac ctcagcagat
     1981 acaactatat acgtttgcat attaaggtct tcaaaccccg tatatttctt tgaaccttgt
     2041 cctttcttac ggacgttatt taggtagtag tgcatcttgt ccgtattaag ccacatctcg
     2101 ttaaatttct ttgtttgaag gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc
     2161 ctatacagcc actccagctt atgatagtag gttccaactc caattcctaa aatatcacaa
     2221 gtacgactga ctgaaaccct tccaacaacc atcttagcga acattggaag aatggtgttc
     2281 ttttgttggt gataagtagt ggtttcttct ctttttggta gaacattagt gaacttctta
     2341 cacgccttac attgatagcg ttgagatttg cccctacttt tccccctttt gtaaaattgc
     2401 ttcggctcat tgaatggggt tgattcttct tccgagcagc cttctttatg aaattgataa
     2461 tctggctcaa catcttggat gctattgatt tctataaggc gtttaatttc ttcaacaaca
     2521 gaccaattag ataaaggggt tactgtacag ttttgcgata cacctctgcc aataggattg
     2581 tcattgcagt acataccttt atgaccttta tctttggaac taccagtcat tttatatcga
     2641 cttggtttcc cttttacatc tttgaatttg tgctgttctt tcccaaaatt acaacaaaac
     2701 ggattgatgc agtaattata ttgaatggga tattcagaac cgttccattt aaaagacgtt
     2761 ggcaagaaca acattttgtt ataccttgtt gcaaatcgtt ttggagttaa taaatcatat
     2821 tgtttagccc tatcttttaa ttcttcgttt gaaatcggaa ttttaacatc tacaatttca
     2881 tcttccttcg tggctaatct tttaagcttg gtcattgtct atcaacccac tctatctttg
     2941 atacccattt tttcaacaat ccgcttatta cttcgcttgt tggcaatcca ttcaataaac
     3001 ttcttatcgt ctaaaatgtt caataagaat tcatccactg cttcgctttc agtacgctct
     3061 gcatattcag cgtattgctt aactatcacc cttacttgct ctgatatttc ccaatccaca
     3121 gacttagcgt tcttattttt tggctcaaga aattgcatag gaaaatgcac ccctttttgc
     3181 ttagtaaatt gtaaccattt taacacacca atttgcattc ctacaataat tatttcacgt
     3241 tcgcattttt atatacattt taacttacat tttgtggtgt aaattaacaa caaacgttga
     3301 tttaacaata aaaaacgagt atcaaattca gttgcaattt gatactcatt taggtttaca
     3361 ttttcacttt ttgtactcca aaactgaact acttaatatg caagacgttg tctttttttt
     3421 gtatattcaa ttttgaaaac atttccgcaa agttcagacg gacctcgatg tgttgatgaa
     3481 taaaatttat tagtaataac ttctataagg ttatcaatcc ctatattact ttttgcactt
     3541 ccatgataaa gagggaacag agaacaattc tgaaatctta tgctttcctc ttgttcgagt
     3601 tcca
//
LOCUS       DAGCYL010000375         3373 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.guided.211, whole genome shotgun sequence.
ACCESSION   DAGCYL010000375 DAGCYL010000000
VERSION     DAGCYL010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.guided.211"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="MB216_002912"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="MB216_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBT4527656.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..>3373
                     /gene="tet(L)"
                     /locus_tag="MB216_002913"
     CDS             2039..>3373
                     /gene="tet(L)"
                     /locus_tag="MB216_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBT4527657.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLV"
BASE COUNT         1071 a          515 c          692 g         1095 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gtt
//
LOCUS       DAGCYL010000376         3373 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.guided.212, whole genome shotgun sequence.
ACCESSION   DAGCYL010000376 DAGCYL010000000
VERSION     DAGCYL010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.guided.212"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="MB216_002914"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="MB216_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBT4527658.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..>3373
                     /gene="tet(L)"
                     /locus_tag="MB216_002915"
     CDS             2039..>3373
                     /gene="tet(L)"
                     /locus_tag="MB216_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBT4527659.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLV"
BASE COUNT         1070 a          515 c          693 g         1095 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gtt
//
LOCUS       DAGCYL010000377         3127 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.guided.217, whole genome shotgun sequence.
ACCESSION   DAGCYL010000377 DAGCYL010000000
VERSION     DAGCYL010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.guided.217"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..293)
                     /locus_tag="MB216_002916"
     CDS             complement(<1..293)
                     /locus_tag="MB216_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBT4527660.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGG"
     gene            655..1695
                     /locus_tag="MB216_002917"
     CDS             655..1695
                     /locus_tag="MB216_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HBT4527661.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQ
                     LKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSR
                     IDKVRYMFNKIT"
     gene            1793..>3127
                     /gene="tet(L)"
                     /locus_tag="MB216_002918"
     CDS             1793..>3127
                     /gene="tet(L)"
                     /locus_tag="MB216_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBT4527662.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLV"
BASE COUNT          958 a          501 c          572 g         1096 t
ORIGIN      
        1 cctccgaaaa taaaaatttc ttcttcgttt ttacataact caaaaacatc ttctattgaa
       61 tgaacaattt cacaaccatt aaaggtaaac cccttatctc tcgtcagaat aatatttctt
      121 ctgtcaggta aggctcttcc gattgattca aggttcttcc tacctaatat tatcggatgt
      181 ccctttgtag tatttttaac atattcccag tccttgggaa tcctccaagg aatgtcattc
      241 tctttgccaa tcactctatt cttatccatc gcagcaatca aagaaacttt catttataac
      301 acagcctttt taactaattt accatctaat ttcttatttc tatcaaaaaa ccttttctca
      361 ttatcctgcc cgttagttga ataagcaatt ttttctataa gttatattat cataatacca
      421 caaaatcaca aattttcatt caattatcat tttcaattgc attttataaa tcattttgta
      481 tgtaatttgg tatgtaagtt aaaatgaaaa aagtagtcca ttttgaagta cattttgtca
      541 ttttgtactc caaaactgaa ctacttactt gcatatatac gcctttatag tggagtacta
      601 catttacgag attcggttag agtatcagaa aaagaaaaaa taaaagttac agaaatgtat
      661 acttcaataa atggtgaatt atgtaagatt gatagagctt attctggaga aattgttatt
      721 ttgcaaaatg agtttttgaa gttaaatagt gttcttggag atacaaaact attgccacag
      781 agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa ctgttgaacc gagtaaacct
      841 gaacagagag aaatgttgct tgatgccctt ttggaaatct cagatagtga tccgcttcta
      901 cgatattacg tggattctac gacacatgaa attatacttt ctttcttagg gaaagtacaa
      961 atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga actaaaagag
     1021 cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat tcacatcgaa
     1081 gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct tccgttggga
     1141 agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc atttcaaaat
     1201 gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg gaatgtgacg
     1261 gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac cccagcagat
     1321 tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg aacagaattg
     1381 ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc acgagcatac
     1441 aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa taatgaggtc
     1501 attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga tttaactttc
     1561 tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt tactaccggt
     1621 gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg atatatgttc
     1681 aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg ttaaataaga
     1741 tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa ttatgaagtg
     1801 taatgaatgt aacagggtta aatacaacca aattttaatt tggctttgcg ttttatcttt
     1861 ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta cctgatattg caaatgattt
     1921 taataaacca cctgcgagta caaactgggt gaacacagcc tttatgttaa ccttttccat
     1981 tggaacagct gtatatggaa agctatctga tcaattaggc atcaaaaggt tactcctatt
     2041 tggaattata ataaattgtt tcgggtcggt aattgggttt gttggccatt ctttcttttc
     2101 cttacttatt atggctcgtt ttattcaagg ggctggtgca gctgcatttc cagcactcgt
     2161 aatggttgta gttgcgcgct atattccaaa ggaaaatagg ggtaaagcat ttggtcttat
     2221 tggatcgata gtagccatgg gagaaggagt cggtccagcg attggtggaa tgatagccca
     2281 ttatattcat tggtcctatc ttctactcat tcctatgata acaattatca ctgttccgtt
     2341 tcttatgaaa ttattaaaga aagaagtaag gataaaaggt cattttgata tcaaaggaat
     2401 tatactaatg tctgtaggca ttgtattttt tatgttgttt acaacatcat atagcatttc
     2461 ttttcttatc gttagcgtgc tgtcattcct gatatttgta aaacatatca ggaaagtaac
     2521 agatcctttt gttgatcccg gattagggaa aaatatacct tttatgattg gagttctttg
     2581 tgggggaatt atatttggaa cagtagcagg gtttgtctct atggttcctt atatgatgaa
     2641 agatgttcac cagctaagta ctgccgaaat cggaagtgta attattttcc ctggaacaat
     2701 gagtgtcatt attttcggct acattggtgg gatacttgtt gatagaagag gtcctttata
     2761 cgtgttaaac atcggagtta catttctttc tgttagcttt ttaactgctt cctttctttt
     2821 agaaacaaca tcatggttca tgacaattat aatcgtattt gttttaggtg ggctttcgtt
     2881 caccaaaaca gttatatcaa caattgtttc aagtagcttg aaacagcagg aagctggtgc
     2941 tggaatgagt ttgcttaact ttaccagctt tttatcagag ggaacaggta ttgcaattgt
     3001 aggtggttta ttatccatac ccttacttga tcaaaggttg ttacctatgg aagttgatca
     3061 gtcaacttat ctgtatagta atttgttatt acttttttca ggaatcattg tcattagttg
     3121 gctggtt
//
LOCUS       DAGCYL010000378         3026 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.guided.220, whole genome shotgun sequence.
ACCESSION   DAGCYL010000378 DAGCYL010000000
VERSION     DAGCYL010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.guided.220"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            47..1018
                     /locus_tag="MB216_002919"
     CDS             47..1018
                     /locus_tag="MB216_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HBT4527663.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            1011..2039
                     /gene="vanB"
                     /locus_tag="MB216_002920"
     CDS             1011..2039
                     /gene="vanB"
                     /locus_tag="MB216_002920"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HBT4527664.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2045..2653
                     /locus_tag="MB216_002921"
     CDS             2045..2653
                     /locus_tag="MB216_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HBT4527665.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(2719..2892)
                     /locus_tag="MB216_002922"
     CDS             complement(2719..2892)
                     /locus_tag="MB216_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527666.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT          814 a          635 c          828 g          749 t
ORIGIN      
        1 gaataaaaac tattagacaa atgatatttt acgaggagga gaaccaatga gaaaaagtgt
       61 gggcattact gtttttggat gcgaacagga tgaggcaaat gctttccgcg ctttatcgcc
      121 ggattttcat attatcccta cgctgattag cgatgcgata tcggcagaca acgcaaaatt
      181 ggccgctggc aatcaatgcg ttagcgtagg ccataagtcc gaggtttccg aggcgacaat
      241 tcttgcgctg agaaaggtcg gggtaaaata catttctacc cgcagcatcg gctgcgatca
      301 cattgatacg actgccgccg agagaatggg aatctcggtt ggcacggttg cgtattcgcc
      361 ggacagcgtt gcggattatg ctttgatgct gatgctgatg gccatacggg gtgcaaaacc
      421 caccatgcac gccgtggcgc aacaagattt cagattggat cgtatccggg ggaaagaact
      481 gggggatatg actgtgggag ttattggaac cggccatatc gggcaagcgg tcgtcaaaag
      541 gctgcgggga tttggatgcc atgtgctggc ctatgataac agccgaaaaa tggatgcaga
      601 ttatgtccag cttgatgagc ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct
      661 ttgtgcggat acccgccatc tgatcggtca gaagcaaatt ggagagatga agcaaggcgc
      721 atttttaatc aacaccgcgc gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt
      781 ggaaagcgga aagctgggtg gtgcggcact ggatgtgttg gagggcgagg atcagtttgt
      841 ttataccgat tgctcgcaga aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat
      901 gccgaatgtg atcatcacac cccatacggc gtactacact gagcgtgtgc tgcaggatac
      961 tacagaaaaa acaatcagga attgtctcaa ttttgaaagg agtttacagc atgaatagaa
     1021 taaaagtcgc aatcatcttc ggcggttgct cggaggaaca tgatgtgtcg gtaaaatccg
     1081 caatagaaat tgctgcgaac attgatacgg aaaaattcga tccgcactac atcggaatta
     1141 caaaaaacgg tgtatggaag ctatgcaaga agccatgtac ggaatgggaa gccgacagtc
     1201 tccccgccat actctccccg gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg
     1261 aatacgaaac acggcgtatt gatgtggctt tcccggtttt gcatggcaaa tgcggggagg
     1321 atggtgcgat acaggggctg tttgtattgt ctggtatccc ctatgtgggc tgtgatattc
     1381 aaagctccgc agcttgcatg gacaaatcac tggcctacat tcttacaaaa aatgcgggca
     1441 tcgccgttcc cgaatttcaa attattgata aaggtgacaa gccggaggcg ggtgcgctta
     1501 cctaccctgt ctttgtgaag ccggcacggt caggttcgtc ctttggcgta accaaagtaa
     1561 acggtacgga agaacttaac gctgcgatag aagcggcagg acaatatgat ggaaaaatct
     1621 taattgagca agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg
     1681 atttgattgt cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc cgcatccatc
     1741 aggaaaacga gccggaaaaa ggctcagaaa atgcgatgat tacagttcac gcagacattc
     1801 cggtcgagga acgaaatcgg gtgcaggaaa cggcaaagaa agtatatcgg gtgcttggat
     1861 gcagagggct tgcccgtgtt gatctttttt tgcaggagga tggcggcatc gttctaaatg
     1921 aggtcaatac catgcccggt tttacatcgt acagccgcta cccacgtatg gtggccgccg
     1981 caggaatcac gcttcctgca ctgattgaca gcctgattac attggcgtta aagaggtgat
     2041 aagtatggaa aatggttttt tgtttttaga tgaaatgttg catggcgtcc gttgggatgc
     2101 caagtacgcc acatgggata acttcacagg aaaaccggtg gatgggtatg aggtgaatcg
     2161 catcatcggc acaaaggccg tggcgtttgc tctgcgcgag gcacaaatcc atgcggctgc
     2221 gcttggctat ggcttgcttt tatgggatgg atatcggcca agaactgcgg tggactgctt
     2281 cctgcgttgg gcagcgcaac cggaggacaa tctcacaaaa gaaaaatttt accccaatat
     2341 agagcgagcc gagttgatta caaagggtta tgtggcctca caatccagcc atagccgtgg
     2401 aagcgcaatt gatcttacgc tctaccacct ggatacaggg gaacttgttt caatgggaag
     2461 taacttcgat tttatggacg aacggtcgca tcatacagca aaagggatag gggatgcaga
     2521 ggcacaaaat cgaagatgct tgcgtaaaat catggaaagc agcggatttc agtcttatcg
     2581 ctttgaatgg tggcactata agttgattga tgagccatac cccgatacct attttaattt
     2641 tgccgtttca taatgaaagt attggttttg tatcagattt tggatttgat aaaatctatg
     2701 ctggtcatgg acaatctttt actggaaagt ggttaaagca tcttttgtaa tacattgcaa
     2761 attatagtat tcaatctgcc cagcggcaga aaagaaaaaa accgctgttg ggcagacctt
     2821 tcccacgccc attttgtatt ttgctggcgg cggttttgag aaatcaaggc cgccgttctt
     2881 ttctggcagc aaaaggtgtg gcggcccata ggaggatgcc gctatgctgt tttgctattg
     2941 ccatgctcta atccacttcc accactcaaa aaagccttgc ccctccatga actgaacccc
     3001 aaaagttgaa ctatttaatg gactgt
//
LOCUS       DAGCYL010000379         2015 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.guided.251, whole genome shotgun sequence.
ACCESSION   DAGCYL010000379 DAGCYL010000000
VERSION     DAGCYL010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.guided.251"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="MB216_002923"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="MB216_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBT4527667.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
BASE COUNT          693 a          310 c          413 g          599 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaatt
//
LOCUS       DAGCYL010000380         2015 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.guided.252, whole genome shotgun sequence.
ACCESSION   DAGCYL010000380 DAGCYL010000000
VERSION     DAGCYL010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.guided.252"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="MB216_002924"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="MB216_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBT4527668.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
BASE COUNT          692 a          310 c          414 g          599 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaatt
//
LOCUS       DAGCYL010000381         1998 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.guided.253, whole genome shotgun sequence.
ACCESSION   DAGCYL010000381 DAGCYL010000000
VERSION     DAGCYL010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.guided.253"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            47..439
                     /locus_tag="MB216_002925"
     CDS             47..439
                     /locus_tag="MB216_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4527669.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            440..1879
                     /locus_tag="MB216_002926"
                     /note="aph(2'')-Ia"
     CDS             440..1879
                     /locus_tag="MB216_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HBT4527670.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          831 a          153 c          343 g          671 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
       61 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      121 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      181 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      241 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      301 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      361 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      421 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      481 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      541 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      601 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      661 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      721 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      781 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      841 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      901 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      961 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1021 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1081 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1141 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1201 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1261 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1321 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1381 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     1441 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
     1501 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
     1561 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     1621 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     1681 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     1741 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
     1801 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
     1861 aaggacttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
     1921 taaatagttt aaaaaaatga aaaataataa aggaagtgag tcaagtccag actcctgtgt
     1981 aaaatgctat acaatgtt
//
LOCUS       DAGCYL010000382         1804 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.guided.266, whole genome shotgun sequence.
ACCESSION   DAGCYL010000382 DAGCYL010000000
VERSION     DAGCYL010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.guided.266"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..331
                     /locus_tag="MB216_002927"
     CDS             <1..331
                     /locus_tag="MB216_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527671.1"
                     /translation="AGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNE
                     VILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVR
                     YMFNKIT"
     gene            429..1802
                     /gene="tet(L)"
                     /locus_tag="MB216_002928"
     CDS             429..1802
                     /gene="tet(L)"
                     /locus_tag="MB216_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBT4527672.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT          529 a          268 c          357 g          650 t
ORIGIN      
        1 agctggaaca gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata
       61 tctttcacga gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt
      121 gaaaaataat gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg
      181 tagtgattta actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta
      241 ccatgttact accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa
      301 agtacgatat atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt
      361 ttcttgttaa ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg
      421 aattaattat gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc
      481 tttgcgtttt atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg
      541 atattgcaaa tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta
      601 tgttaacctt ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca
      661 aaaggttact cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg
      721 gccattcttt cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg
      781 catttccagc actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta
      841 aagcatttgg tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg
      901 gtggaatgat agcccattat attcattggt cctatcttct actcattcct atgataacaa
      961 ttatcactgt tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt
     1021 ttgatatcaa aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa
     1081 catcatatag catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac
     1141 atatcaggaa agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta
     1201 tgattggagt tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg
     1261 ttccttatat gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta
     1321 ttttccctgg aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata
     1381 gaagaggtcc tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa
     1441 ctgcttcctt tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt
     1501 taggtgggct ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac
     1561 agcaggaagc tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa
     1621 caggtattgc aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac
     1681 ctatggaagt tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa
     1741 tcattgtcat tagttggctg gttaccttga atgtatataa acattctcaa agggatttct
     1801 aaat
//
LOCUS       DAGCYL010000383         1769 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.guided.271, whole genome shotgun sequence.
ACCESSION   DAGCYL010000383 DAGCYL010000000
VERSION     DAGCYL010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.guided.271"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..293)
                     /locus_tag="MB216_002929"
     CDS             complement(<1..293)
                     /locus_tag="MB216_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBT4527673.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGG"
     gene            655..1695
                     /locus_tag="MB216_002930"
     CDS             655..1695
                     /locus_tag="MB216_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBT4527674.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
BASE COUNT          580 a          296 c          293 g          600 t
ORIGIN      
        1 cctccgaaaa taaaaatttc ttcttcgttt ttacataact caaaaacatc ttctattgaa
       61 tgaacaattt cacaaccatt aaaggtaaac cccttatctc tcgtcagaat aatatttctt
      121 ctgtcaggta aggctcttcc gattgattca aggttcttcc tacctaatat tatcggatgt
      181 ccctttgtag tatttttaac atattcccag tccttgggaa tcctccaagg aatgtcattc
      241 tctttgccaa tcactctatt cttatccatc gcagcaatca aagaaacttt catttataac
      301 acagcctttt taactaattt accatctaat ttcttatttc tatcaaaaaa ccttttctca
      361 ttatcctgcc cgttagttga ataagcaatt ttttctataa gttatattat cataatacca
      421 caaaatcaca aattttcatt caattatcat tttcaattgc attttataaa tcattttgta
      481 tgtaatttgg tatgtaagtt aaaatgaaaa aagtagtcca ttttgaagta cattttgtca
      541 ttttgtactc caaaactgaa ctacttactt gcatatatac gcctttatag tggagtacta
      601 catttacgag attcggttag agtatcagaa aaagaaaaaa taaaagttac agaaatgtat
      661 acttcaataa atggtgaatt atgtaagatt gatagagctt attctggaga aattgttatt
      721 ttgcaaaatg agtttttgaa gttaaatagt gttcttggag atacaaaact attgccacag
      781 agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa ctgttgaacc gagtaaacct
      841 gaacagagag aaatgttgct tgatgccctt ttggaaatct cagatagtga tccgcttcta
      901 cgatattacg tggattctac gacacatgaa attatacttt ctttcttagg gaaagtacaa
      961 atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga actaaaagag
     1021 cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat tcacatcgaa
     1081 gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct tccgttggga
     1141 agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc atttcaaaat
     1201 gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg gaatgtgacg
     1261 gattgtaaaa tctgttttaa gtacggttta tactatagcc ctgttagtac tccagcagat
     1321 tttcggatgc ttactcctat tgtactggag caagccttta gaaaagctgg aacagaattg
     1381 ttagagccat atcttagttt taaagtttat gcaccacagg aatatctttc acgggcatat
     1441 aacgatgctc ccaaatattg tgcaaatatc gtaaatactc aactgaaaaa taatgaggtc
     1501 attattattg gagaaattcc tgctcgatgt attcaagatt atcgcaatga tttaactttt
     1561 tttacaaatg ggcttagtgt ttgtttagca gagctaaaag gatatcaggt taccactggc
     1621 gaacctgttt gccagacccg tcgtctaaat agtcggatag ataaagtaag atatatgttc
     1681 aataaaataa cttagtgcgt tttatgttgt tatataaata tggtttctta ttaaataaga
     1741 tgaaatattc tttaatatag atttgaatt
//
LOCUS       DAGCYL010000384         1166 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.guided.306, whole genome shotgun sequence.
ACCESSION   DAGCYL010000384 DAGCYL010000000
VERSION     DAGCYL010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.guided.306"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..167
                     /locus_tag="MB216_002931"
     CDS             <1..167
                     /locus_tag="MB216_002931"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4527675.1"
                     /translation="LFDSRFKTLQQNPKWPLEDRNLADTALWLNAEFMKNAAEIGYAR
                     FLYGNSMKQK"
     gene            236..1147
                     /gene="aph(2'')"
                     /locus_tag="MB216_002932"
     CDS             236..1147
                     /gene="aph(2'')"
                     /locus_tag="MB216_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021401024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ie/IVa family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HBT4527676.1"
                     /translation="MTTYTFDQVEEAIEQLYPDFTINTIEISGEGNDCIAYEINGNFI
                     FKFPKHSRASINLLNEVTVLKTIHNELSLPIPEVVFTGMPSEMCQMSFAGFTKIKGVP
                     LTPLLLKNLPKQSQDQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKL
                     LSRELKGHQMKKVDDFYRDILDNEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDF
                     GDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYMMKEKYWSFEKI
                     IYGKEYGYMDWYEEGLNEIRSSELLTT"
BASE COUNT          437 a          177 c          215 g          337 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc aaaatggcca ttagaagata
       61 ggaacttagc agatactgca ctttggctaa atgcagaatt tatgaaaaat gcagcagaga
      121 taggttatgc aagattttta tatggaaata gcatgaaaca aaaatgaatt aactataatt
      181 gctaaattct tatttacagt caatcagatt tttgattaaa gggagtagag aagacatgac
      241 aacttatact ttcgaccagg tagaagaggc aatagagcag ttatatcctg attttactat
      301 caatacaata gagatttcag gagaaggcaa tgactgtatt gcatatgaaa taaacgggaa
      361 ttttattttt aaatttccaa agcattcaag agcttcgatt aatctcttga atgaagtaac
      421 cgtactcaaa acaatccaca atgaattatc actacccatt cccgaggtgg tttttacagg
      481 aatgccatca gaaatgtgcc aaatgtcttt cgcaggtttt acaaaaatta aaggagtacc
      541 tttgacacct cttctactca aaaatctgcc gaagcaatct caagatcagg cagctaagga
      601 cctggcccga tttttaagtg aacttcacag cataaatatc tctggattca aaagtaatct
      661 ggtattagat tttcgagaga agataaatga agataataaa aaaatcaaaa agttactatc
      721 cagggaatta aagggtcacc agatgaagaa agtggatgat ttttacaggg atattctaga
      781 caacgaaatc tacttcaaat actatccttg tcttattcat aacgatttca gcagcgatca
      841 tattttattt gataccgaaa aaaataccat ttgtggaata atcgattttg gagatgcagc
      901 tatttctgat cccgacaatg attttataag tttgatggaa gatgatgaag agtacggcat
      961 ggaattcgta tcaaaaatat tgaaccatta caaacataag gatataccga cagttttgga
     1021 aaaatatatg atgaaagaaa aatactggtc gttcgaaaag attatctatg gaaaggaata
     1081 tggttatatg gattggtatg aagagggatt aaatgaaatc agaagcagtg aactactcac
     1141 cacttagcta aacctgacgg ttctta
//
LOCUS       DAGCYL010000385          950 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.guided.326, whole genome shotgun sequence.
ACCESSION   DAGCYL010000385 DAGCYL010000000
VERSION     DAGCYL010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.guided.326"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            337..885
                     /gene="aac(6')"
                     /locus_tag="MB216_002933"
     CDS             337..885
                     /gene="aac(6')"
                     /locus_tag="MB216_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBT4527677.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          338 a          137 c          190 g          285 t
ORIGIN      
        1 taccgattcc ctttgctttt ttgtaattat ctaaaaattc taaaaattaa gattattata
       61 tgaaaacagg ttttatttgt caattcataa ttggtttttt ttaaagcaaa agataatcta
      121 tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga tctttcctat
      181 tttgaataaa gtgtttctgt ataatttttt tattttataa gtaagataaa taatgaaaga
      241 atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt ttttcaaaag
      301 gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga atttgaccgt
      361 aataatccag tattgaaaga tcagctttct gatttactga gactgacttg gccggaagaa
      421 tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg aatcgcggta
      481 gcagcggtag accaagatga gttagtagga tttattggtg caatccctca atacggtatc
      541 acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa ccaaataggt
      601 actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat cacgatttat
      661 ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct gtatgagcat
      721 acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga attctatgaa
      781 aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga caaaccggat
      841 atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa agaggttgtg
      901 acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt
//
LOCUS       DAGCYL010000386          870 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911144
            SAMN25126072-rid13883083.guided.332, whole genome shotgun sequence.
ACCESSION   DAGCYL010000386 DAGCYL010000000
VERSION     DAGCYL010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126072
            Sequence Read Archive: SRR17671403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242190.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242190.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:38:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,934
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,811
            CDSs (with protein)               :: 2,811
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 21 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126072-rid13883083.guided.332"
                     /strain="M911144"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            32..766
                     /locus_tag="MB216_002934"
     CDS             32..766
                     /locus_tag="MB216_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HBT4527678.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          354 a          117 c          118 g          281 t
ORIGIN      
        1 gttaatataa acctcatagg agtgggttat aatgaacaaa aaaaatataa aagatagtca
       61 aaactttatt acttcaaagc atcatataaa tgaaattttg agaaatgtac atttaaatac
      121 aaatgataat attattgaga ttggttcagg gaaaggtcat ttcacgtttg aattagctaa
      181 aaggtgtaat tatgtaaccg ccattgaaat agatcctaaa ttatgtagga taactaaaaa
      241 caaacttatt gaatatgaga acttccaggt tatcaataaa gatattttac aatttaagtt
      301 tcctaaaaat aagtcatata agatatatgg aaatataccc tacaatataa gtacagatat
      361 aattcgaaaa attgtttttg aaagcacagc tacagaaagt tatttaatag tggaatatgg
      421 atttgctaaa aggttgctaa atacaaatcg ttcactagca ctatttttaa tgacagaagt
      481 tgatatatcc atattaagta aaatccctag agaatacttt catccaaaac ctagagttaa
      541 tagctcgtta attgtattaa aaagacaccc ttcaaaaata tcactcaaag atagaaaaca
      601 atatgaaaat tttgttatga aatgggttaa caaagaatac ataaaactat tttccaaaaa
      661 ccaattttat caagccttaa aatatgcaag aattgacgat ttaaacaata ttagctttga
      721 acaattcttg tctcttttca atagctataa attatttaat agataagtta aggggtgcat
      781 aaactgcatc ccttaactta tcttattttg cttaaacaat cttggcccgt aaactgcata
      841 actcatatgt aaatcgctcc tttttaggtg
//
